CmoCh01G018990 (gene) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh01G018990
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
Descriptionabscisic acid 8'-hydroxylase 4-like
LocationCmo_Chr01: 13671091 .. 13673473 (+)
RNA-Seq ExpressionCmoCh01G018990
SyntenyCmoCh01G018990
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CTGCTCTCTGTATAAAACCCCCCTGCTTCTCAGAGAATCACTGTAATTACAACCAAGAATAAAATTGTGTTTAACCAATTTCAGAGAGAAAGTGTCGGCGGAAACAACTGCAGGAAGGGGGAGTAAATGGGTGTAATTTTTGCATCTCTTTTGCTATTTTTTCTCATTGTATTGTCGTATATTTATTTAAACAGAAACAAGAGGGCAGGCATTAGAGATGGAGCTAAGCTCCCTCCTGGTTCTTTAGGGTGGCCTTATATTGGAGAAACCCTTCAGCTCTATTCTCAAGACCCTGATTTGTTCTTCGCCGCCAAACAGAAAAGGTTGCGCCTTTTTGTTTAGTTTTGAGTGTCTTGTTTGCTAAGGTGGGTTGGTGCTGACTTGTACGTGGTTTGTTTGTTTTTTTAGGTATGGGGAGATTTTTAAGACGCATATACTTGGATGTCCTTGCGTGATGTTAGCTAGCCCCGAGGCGGCGAGGTTCGTGCTGGTGACACAGGCTCATTTGTTTAAGCCTACTTACCCCAAAAGCAAAGAGCGTTTGATCGGCCCATCTGCCTTGTTTTATCACCAAGGTGACTATCATTGCCGCCTTAGGAAGCTGGTTCAGAGCTCTCTATCTTTGGACGTCATCCGGAGCCTGGTCCCCGATATCGACTCCGTAACCACCTCCGCTCTCCACTCCTGGACCGGTGCCCACCTTATGAACACTTTTCATGAGATGAAAAAGGTTGGTTTGGTTTGGTTTCTTTTTAATCCTGGGCCGTATAGTTATTGGGTCGGTCACTTTTTTAAGTCTTGAAATTTGTGTTGTTTAGATATCCTTTGAAGTTGGGATATTGGCCATTTTTGGGCATTTAGAAGCTGCTTACAAAGAGGACCTGAAAAAAAACTACAGCATTTTGGACGCAGGCTACAATTCTTTCCCCACCAACATTCCTGGAACTCCATACAAAAAGGCACTCTCGGTAAGTTAAAACCTAAGGAGAAGATGGAAAATTATTATGTTATATTATATATATATTTATGTTAAATTAGTTGTCCCCTTGGACAGGCTAGAAAGCGGCTAAGTAAAATCTTAGGTGATATAATCCACGAAAGGAGGGAGAAGAAGGTGGGGAAGAAGGATCTTTTGGGGTGCTTTCTGAACTCCATAGATGAAGAAGGTGAAGCTATAAGCGATGCCCAAATGGAAGATAACATAATTGGGGTGCTTTTTGCGGCTCAAGACACCACAGCCAGTGTCATGACTTGGGTCGTCAAGTACCTTTATGACCAACCCAAGCTTCTTCAAACGCTAAAAGTGAGTAGTTACGTACCCTTAAAAAAAAAGGTAATTTTTTTTATTCAAAATCATGATTATTTGTTTAAAAAATCATTTTTTTTCGTTTAGAAAGAGCAACAGGAAATTTTAGAGTTCAATTACGAGAAAAATCAACCGTTGAACTGGGTTCAGACAAGAAACATGCCCATGAGTTATAAGGTAAATAAATTTATGGTAACCCATCTAGGGGTGATTAAGATGGACGATTTAGGAATAAATAATCTTTAATTCAGGTAGTATTGGAGAGCTTAAGGATGGCGAGCATTATATCATTCACATTCAGGGAGGCTGTAGCTGACGTGGAATACAAAGGTAAGGGAGTAGACCCAAAGAGAGAGTTGTTGATGGAAGTTTTTTTTTTTAATATATATATATATTTTTTAAAATAATAAAATGATTTGAGTTTGTGAAATGACGTGGAATAGGGTATTTGATTCCAAAAGGTTGGAAAGTGATGCCTTTGTTTCGAAACATCCATCACAGTCCCGAATATTTTGCCGATCCTCACAAATTCGACCCTTCTAGATTTGAGGTAAATTAAAATTTGAATGTTAATATTTTTGTGTATAATGGATATTTGAATAATAATTAGGGAAAATATGGGCGTTTCAAATTTCAGGTTGCACCGAAACCCAATACGTTCATGCCATTTGGCAGTGGAGTTCATACCTGCCCAGGGAACGAGCTTGCCAAGCTGGAGATCTTGATCCTGATTCATCATCTAGTCACCAAATTCAGGTACTGAAATTTGAAAACTGTTTTGGACTTTTTGAGTAATTATTTAAATTATTTATCTTTTGCTGAGTTGGCAGGTGGGAATTAGTTGGTTCGCAAAACGGGGTTCAGCATAGTCCATTTCCCGTTCCCTCTCAAGGCCTTCCAGCACGATTTTGGAACATAGCCCACCACAACACCGTACATACCAAAATGGACCCCAACTAAGTGGACCAATCTTCCACCCAATAGCAAAACCCAAGCCCGAGTCCAAATACAAGTCCAAGCCCAAGCCCAAGCCCATCTTCTTTTTTTATTTTCAGTTTTTCTTATAATTAGGCCAGT

mRNA sequence

CTGCTCTCTGTATAAAACCCCCCTGCTTCTCAGAGAATCACTGTAATTACAACCAAGAATAAAATTGTGTTTAACCAATTTCAGAGAGAAAGTGTCGGCGGAAACAACTGCAGGAAGGGGGAGTAAATGGGTGTAATTTTTGCATCTCTTTTGCTATTTTTTCTCATTGTATTGTCGTATATTTATTTAAACAGAAACAAGAGGGCAGGCATTAGAGATGGAGCTAAGCTCCCTCCTGGTTCTTTAGGGTGGCCTTATATTGGAGAAACCCTTCAGCTCTATTCTCAAGACCCTGATTTGTTCTTCGCCGCCAAACAGAAAAGGTATGGGGAGATTTTTAAGACGCATATACTTGGATGTCCTTGCGTGATGTTAGCTAGCCCCGAGGCGGCGAGGTTCGTGCTGGTGACACAGGCTCATTTGTTTAAGCCTACTTACCCCAAAAGCAAAGAGCGTTTGATCGGCCCATCTGCCTTGTTTTATCACCAAGGTGACTATCATTGCCGCCTTAGGAAGCTGGTTCAGAGCTCTCTATCTTTGGACGTCATCCGGAGCCTGGTCCCCGATATCGACTCCGTAACCACCTCCGCTCTCCACTCCTGGACCGGTGCCCACCTTATGAACACTTTTCATGAGATGAAAAAGATATCCTTTGAAGTTGGGATATTGGCCATTTTTGGGCATTTAGAAGCTGCTTACAAAGAGGACCTGAAAAAAAACTACAGCATTTTGGACGCAGGCTACAATTCTTTCCCCACCAACATTCCTGGAACTCCATACAAAAAGGCACTCTCGGCTAGAAAGCGGCTAAGTAAAATCTTAGGTGATATAATCCACGAAAGGAGGGAGAAGAAGGTGGGGAAGAAGGATCTTTTGGGGTGCTTTCTGAACTCCATAGATGAAGAAGGTGAAGCTATAAGCGATGCCCAAATGGAAGATAACATAATTGGGGTGCTTTTTGCGGCTCAAGACACCACAGCCAGTGTCATGACTTGGGTCGTCAAGTACCTTTATGACCAACCCAAGCTTCTTCAAACGCTAAAAAAAGAGCAACAGGAAATTTTAGAGTTCAATTACGAGAAAAATCAACCGTTGAACTGGGTTCAGACAAGAAACATGCCCATGAGTTATAAGGTAGTATTGGAGAGCTTAAGGATGGCGAGCATTATATCATTCACATTCAGGGAGGCTGTAGCTGACGTGGAATACAAAGGGTATTTGATTCCAAAAGGTTGGAAAGTGATGCCTTTGTTTCGAAACATCCATCACAGTCCCGAATATTTTGCCGATCCTCACAAATTCGACCCTTCTAGATTTGAGGGAAAATATGGGCGTTTCAAATTTCAGGTTGCACCGAAACCCAATACGTTCATGCCATTTGGCAGTGGAGTTCATACCTGCCCAGGGAACGAGCTTGCCAAGCTGGAGATCTTGATCCTGATTCATCATCTAGTCACCAAATTCAGGTGGGAATTAGTTGGTTCGCAAAACGGGGTTCAGCATAGTCCATTTCCCGTTCCCTCTCAAGGCCTTCCAGCACGATTTTGGAACATAGCCCACCACAACACCGTACATACCAAAATGGACCCCAACTAAGTGGACCAATCTTCCACCCAATAGCAAAACCCAAGCCCGAGTCCAAATACAAGTCCAAGCCCAAGCCCAAGCCCATCTTCTTTTTTTATTTTCAGTTTTTCTTATAATTAGGCCAGT

Coding sequence (CDS)

ATGGGTGTAATTTTTGCATCTCTTTTGCTATTTTTTCTCATTGTATTGTCGTATATTTATTTAAACAGAAACAAGAGGGCAGGCATTAGAGATGGAGCTAAGCTCCCTCCTGGTTCTTTAGGGTGGCCTTATATTGGAGAAACCCTTCAGCTCTATTCTCAAGACCCTGATTTGTTCTTCGCCGCCAAACAGAAAAGGTATGGGGAGATTTTTAAGACGCATATACTTGGATGTCCTTGCGTGATGTTAGCTAGCCCCGAGGCGGCGAGGTTCGTGCTGGTGACACAGGCTCATTTGTTTAAGCCTACTTACCCCAAAAGCAAAGAGCGTTTGATCGGCCCATCTGCCTTGTTTTATCACCAAGGTGACTATCATTGCCGCCTTAGGAAGCTGGTTCAGAGCTCTCTATCTTTGGACGTCATCCGGAGCCTGGTCCCCGATATCGACTCCGTAACCACCTCCGCTCTCCACTCCTGGACCGGTGCCCACCTTATGAACACTTTTCATGAGATGAAAAAGATATCCTTTGAAGTTGGGATATTGGCCATTTTTGGGCATTTAGAAGCTGCTTACAAAGAGGACCTGAAAAAAAACTACAGCATTTTGGACGCAGGCTACAATTCTTTCCCCACCAACATTCCTGGAACTCCATACAAAAAGGCACTCTCGGCTAGAAAGCGGCTAAGTAAAATCTTAGGTGATATAATCCACGAAAGGAGGGAGAAGAAGGTGGGGAAGAAGGATCTTTTGGGGTGCTTTCTGAACTCCATAGATGAAGAAGGTGAAGCTATAAGCGATGCCCAAATGGAAGATAACATAATTGGGGTGCTTTTTGCGGCTCAAGACACCACAGCCAGTGTCATGACTTGGGTCGTCAAGTACCTTTATGACCAACCCAAGCTTCTTCAAACGCTAAAAAAAGAGCAACAGGAAATTTTAGAGTTCAATTACGAGAAAAATCAACCGTTGAACTGGGTTCAGACAAGAAACATGCCCATGAGTTATAAGGTAGTATTGGAGAGCTTAAGGATGGCGAGCATTATATCATTCACATTCAGGGAGGCTGTAGCTGACGTGGAATACAAAGGGTATTTGATTCCAAAAGGTTGGAAAGTGATGCCTTTGTTTCGAAACATCCATCACAGTCCCGAATATTTTGCCGATCCTCACAAATTCGACCCTTCTAGATTTGAGGGAAAATATGGGCGTTTCAAATTTCAGGTTGCACCGAAACCCAATACGTTCATGCCATTTGGCAGTGGAGTTCATACCTGCCCAGGGAACGAGCTTGCCAAGCTGGAGATCTTGATCCTGATTCATCATCTAGTCACCAAATTCAGGTGGGAATTAGTTGGTTCGCAAAACGGGGTTCAGCATAGTCCATTTCCCGTTCCCTCTCAAGGCCTTCCAGCACGATTTTGGAACATAGCCCACCACAACACCGTACATACCAAAATGGACCCCAACTAA

Protein sequence

MGVIFASLLLFFLIVLSYIYLNRNKRAGIRDGAKLPPGSLGWPYIGETLQLYSQDPDLFFAAKQKRYGEIFKTHILGCPCVMLASPEAARFVLVTQAHLFKPTYPKSKERLIGPSALFYHQGDYHCRLRKLVQSSLSLDVIRSLVPDIDSVTTSALHSWTGAHLMNTFHEMKKISFEVGILAIFGHLEAAYKEDLKKNYSILDAGYNSFPTNIPGTPYKKALSARKRLSKILGDIIHERREKKVGKKDLLGCFLNSIDEEGEAISDAQMEDNIIGVLFAAQDTTASVMTWVVKYLYDQPKLLQTLKKEQQEILEFNYEKNQPLNWVQTRNMPMSYKVVLESLRMASIISFTFREAVADVEYKGYLIPKGWKVMPLFRNIHHSPEYFADPHKFDPSRFEGKYGRFKFQVAPKPNTFMPFGSGVHTCPGNELAKLEILILIHHLVTKFRWELVGSQNGVQHSPFPVPSQGLPARFWNIAHHNTVHTKMDPN
Homology
BLAST of CmoCh01G018990 vs. ExPASy Swiss-Prot
Match: A9QNE7 (Abscisic acid 8'-hydroxylase CYP707A1 OS=Solanum lycopersicum OX=4081 GN=CYP707A1 PE=2 SV=1)

HSP 1 Score: 660.2 bits (1702), Expect = 1.8e-188
Identity = 320/474 (67.51%), Postives = 383/474 (80.80%), Query Frame = 0

Query: 4   IFASLLLFFLIVLSYIYL--NRNKRAGIRDGAKLPPGSLGWPYIGETLQLYSQDPDLFFA 63
           IF  + +F L  LSY +     N  +  ++  KLPPGS+GWPYIGETLQLYSQDP+ FF 
Sbjct: 7   IFLYISMFVLGYLSYYFCFGKNNNSSSKKNAYKLPPGSMGWPYIGETLQLYSQDPNAFFI 66

Query: 64  AKQKRYGEIFKTHILGCPCVMLASPEAARFVLVTQAHLFKPTYPKSKERLIGPSALFYHQ 123
            +Q+R+GEIFKT ILGCPCVMLASPEAARFVLV QA+LFKPTYPKSKE LIG SA+F+HQ
Sbjct: 67  NRQRRFGEIFKTKILGCPCVMLASPEAARFVLVNQANLFKPTYPKSKENLIGQSAIFFHQ 126

Query: 124 GDYHCRLRKLVQSSLSLDVIRSLVPDIDSVTTSALHSWTGAHLMNTFHEMKKISFEVGIL 183
           GDYH  LRKLVQ+ L+ + IR+ +P I+ ++ SAL+SW G H++NT+HEMKK SFEVGIL
Sbjct: 127 GDYHNHLRKLVQAPLNPESIRNQIPYIEELSISALNSWVGGHVVNTYHEMKKFSFEVGIL 186

Query: 184 AIFGHLEAAYKEDLKKNYSILDAGYNSFPTNIPGTPYKKALSARKRLSKILGDIIHERRE 243
           AIFGHL+   KE+LKKNYSI+D GYNSFP N+PGT Y+KAL ARK+L KIL +II E +E
Sbjct: 187 AIFGHLDGHVKEELKKNYSIVDKGYNSFPINLPGTLYRKALQARKKLGKILSEIIREMKE 246

Query: 244 KKVGKKDLLGCFLNSIDEEG-EAISDAQMEDNIIGVLFAAQDTTASVMTWVVKYLYDQPK 303
           KK  +K LL CFLN+ +E+G   +++ Q+ DNIIGVLFAAQDTTASV+TW++KYL+D PK
Sbjct: 247 KKTLEKGLLSCFLNAKEEKGFLVLNEDQIADNIIGVLFAAQDTTASVLTWIIKYLHDNPK 306

Query: 304 LLQTLKKEQQEILEFNYEKNQPLNWVQTRNMPMSYKVVLESLRMASIISFTFREAVADVE 363
           LL+ +K EQ+ I + N ++N  L W QTR MP++ +VVLE+LRMASIISF FREAVADVE
Sbjct: 307 LLECVKAEQKVIWQSNEQENHGLTWTQTRKMPITSRVVLETLRMASIISFAFREAVADVE 366

Query: 364 YKGYLIPKGWKVMPLFRNIHHSPEYFADPHKFDPSRFEGKYGRFKFQVAPKPNTFMPFGS 423
           YKGYLIPKGWKVMPLFRNIHH+PE+F DP KFDPSRFE          APKPNTFMPFGS
Sbjct: 367 YKGYLIPKGWKVMPLFRNIHHNPEFFPDPQKFDPSRFEN---------APKPNTFMPFGS 426

Query: 424 GVHTCPGNELAKLEILILIHHLVTKFRWELVGSQNGVQHSPFPVPSQGLPARFW 475
           GVH CPGNELAKLEILI+ HHLVTKFRWE+VGS +G+Q+ PFPVP  GL ARFW
Sbjct: 427 GVHACPGNELAKLEILIMTHHLVTKFRWEVVGSGSGIQYGPFPVPLGGLAARFW 471

BLAST of CmoCh01G018990 vs. ExPASy Swiss-Prot
Match: Q9LJK2 (Abscisic acid 8'-hydroxylase 4 OS=Arabidopsis thaliana OX=3702 GN=CYP707A4 PE=2 SV=2)

HSP 1 Score: 633.6 bits (1633), Expect = 1.8e-180
Identity = 299/465 (64.30%), Postives = 371/465 (79.78%), Query Frame = 0

Query: 9   LLFFLIVLSYIYLNRNKRAGIRDGAKLPPGSLGWPYIGETLQLYSQDPDLFFAAKQKRYG 68
           +L   ++L  + +++ K+       KLPPGS+GWPY+GETLQLYSQ+P++FF +KQKRYG
Sbjct: 11  ILILCLLLVRVIVSKKKK---NSRGKLPPGSMGWPYLGETLQLYSQNPNVFFTSKQKRYG 70

Query: 69  EIFKTHILGCPCVMLASPEAARFVLVTQAHLFKPTYPKSKERLIGPSALFYHQGDYHCRL 128
           EIFKT ILG PCVMLASPEAARFVLVT AH+FKPTYP+SKE+LIGPSALF+HQGDYH  +
Sbjct: 71  EIFKTRILGYPCVMLASPEAARFVLVTHAHMFKPTYPRSKEKLIGPSALFFHQGDYHSHI 130

Query: 129 RKLVQSSLSLDVIRSLVPDIDSVTTSALHSWTGAHLMNTFHEMKKISFEVGILAIFGHLE 188
           RKLVQSS   + IR L+PDI+ +  S+L SW    +++T+ EMKK +F+VGILAIFGHLE
Sbjct: 131 RKLVQSSFYPETIRKLIPDIEHIALSSLQSWANMPIVSTYQEMKKFAFDVGILAIFGHLE 190

Query: 189 AAYKEDLKKNYSILDAGYNSFPTNIPGTPYKKALSARKRLSKILGDIIHERREKKVGKKD 248
           ++YKE LK NY+I+D GYNSFP ++PGT Y KAL ARK+L  I+ +II ERREK+  + D
Sbjct: 191 SSYKEILKHNYNIVDKGYNSFPMSLPGTSYHKALMARKQLKTIVSEIICERREKRALQTD 250

Query: 249 LLGCFLNSIDEEGEAISDAQMEDNIIGVLFAAQDTTASVMTWVVKYLYDQPKLLQTLKKE 308
            LG  LN  +E+G  ++  Q+ DNIIGVLFAAQDTTAS +TW++KYL+D  KLL+ +K E
Sbjct: 251 FLGHLLNFKNEKGRVLTQEQIADNIIGVLFAAQDTTASCLTWILKYLHDDQKLLEAVKAE 310

Query: 309 QQEILEFNYEKNQPLNWVQTRNMPMSYKVVLESLRMASIISFTFREAVADVEYKGYLIPK 368
           Q+ I E N  + +PL W QTRNMP+++KV++ESLRMASIISFTFREAV DVEYKGYLIPK
Sbjct: 311 QKAIYEENSREKKPLTWRQTRNMPLTHKVIVESLRMASIISFTFREAVVDVEYKGYLIPK 370

Query: 369 GWKVMPLFRNIHHSPEYFADPHKFDPSRFEGKYGRFKFQVAPKPNTFMPFGSGVHTCPGN 428
           GWKVMPLFRNIHH+P+YF++P  FDPSRFE         V PKPNTFMPFGSGVH CPGN
Sbjct: 371 GWKVMPLFRNIHHNPKYFSNPEVFDPSRFE---------VNPKPNTFMPFGSGVHACPGN 430

Query: 429 ELAKLEILILIHHLVTKFRWELVGSQNGVQHSPFPVPSQGLPARF 474
           ELAKL+ILI +HHLV+ FRWE+ G + G+Q+SPFP+P  GLPA F
Sbjct: 431 ELAKLQILIFLHHLVSNFRWEVKGGEKGIQYSPFPIPQNGLPATF 463

BLAST of CmoCh01G018990 vs. ExPASy Swiss-Prot
Match: Q0J185 (Abscisic acid 8'-hydroxylase 3 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP707A7 PE=2 SV=1)

HSP 1 Score: 625.9 bits (1613), Expect = 3.7e-178
Identity = 303/477 (63.52%), Postives = 376/477 (78.83%), Query Frame = 0

Query: 8   LLLFFLIVLSYI----YLNRNKRA----GIRDGAKLPPGSLGWPYIGETLQLYSQDPDLF 67
           +++ F I L+++    Y +R +R     G     +LPPGS+GWPYIGETLQLYSQDP++F
Sbjct: 9   IVISFFISLAFMCYVHYTSRQRRKLHGYGHEKAVRLPPGSMGWPYIGETLQLYSQDPNVF 68

Query: 68  FAAKQKRYGEIFKTHILGCPCVMLASPEAARFVLVTQAHLFKPTYPKSKERLIGPSALFY 127
           FA+KQKRYGEIFKTHILGCPCVMLASPEAARFVLVTQAHLFKPTYP+SKER+IGPSALF+
Sbjct: 69  FASKQKRYGEIFKTHILGCPCVMLASPEAARFVLVTQAHLFKPTYPRSKERMIGPSALFF 128

Query: 128 HQGDYHCRLRKLVQSSLSLDVIRSLVPDIDSVTTSALHSWTGAHLMNTFHEMKKISFEVG 187
           HQGDYH RLRKLVQ  L  D +R+LVPD+++   S L SW G ++ +TFH MK++SF+VG
Sbjct: 129 HQGDYHLRLRKLVQGPLGPDALRALVPDVEAAVRSTLASWDG-NVSSTFHAMKRLSFDVG 188

Query: 188 ILAIF-GHLEAAYKEDLKKNYSILDAGYNSFPTNIPGTPYKKALSARKRLSKILGDIIHE 247
           I+ IF G L+   K +L++NY+I++ GYNSFP + PGT Y KA+ AR+RL  +L DI+ E
Sbjct: 189 IVTIFGGRLDERRKAELRQNYAIVEKGYNSFPNSFPGTLYYKAIQARRRLHGVLSDIMRE 248

Query: 248 RREKKVGKKDLLGCFLNS-IDEEGEAISDAQMEDNIIGVLFAAQDTTASVMTWVVKYLYD 307
           RR +     DLLGC + S   ++G  ++D Q+ DNIIGVLFAAQDTTASV+TW+VKYL+D
Sbjct: 249 RRARGEPGSDLLGCLMQSRAGDDGALLTDEQVADNIIGVLFAAQDTTASVLTWIVKYLHD 308

Query: 308 QPKLLQTLKKEQQEILEFNYEKNQPLNWVQTRNMPMSYKVVLESLRMASIISFTFREAVA 367
            PKLL+ ++ EQ  I   N     PL W QTR+M +++KV+LESLRMASIISFTFREAVA
Sbjct: 309 HPKLLEAVRAEQAAIRAANDGGRLPLTWAQTRSMALTHKVILESLRMASIISFTFREAVA 368

Query: 368 DVEYKGYLIPKGWKVMPLFRNIHHSPEYFADPHKFDPSRFEGKYGRFKFQVAPKPNTFMP 427
           DVEYKG+LIPKGWKVMPLFRNIHH+P+YF DP KFDPSR         F+V+P+PNTFMP
Sbjct: 369 DVEYKGFLIPKGWKVMPLFRNIHHNPDYFQDPQKFDPSR---------FKVSPRPNTFMP 428

Query: 428 FGSGVHTCPGNELAKLEILILIHHLVTKFRWELVGSQNGVQHSPFPVPSQGLPARFW 475
           FG+GVH CPGNELAKLE+L+LIHHLVT +RWE+VGS + V++SPFPVP  GL A+ W
Sbjct: 429 FGNGVHACPGNELAKLEMLVLIHHLVTGYRWEIVGSSDEVEYSPFPVPKHGLLAKLW 475

BLAST of CmoCh01G018990 vs. ExPASy Swiss-Prot
Match: A2Z212 (Abscisic acid 8'-hydroxylase 3 OS=Oryza sativa subsp. indica OX=39946 GN=CYP707A7 PE=3 SV=1)

HSP 1 Score: 623.2 bits (1606), Expect = 2.4e-177
Identity = 302/477 (63.31%), Postives = 376/477 (78.83%), Query Frame = 0

Query: 8   LLLFFLIVLSYI----YLNRNKRA----GIRDGAKLPPGSLGWPYIGETLQLYSQDPDLF 67
           +++ F I L+++    Y +R +R     G     +LPPGS+GWPYIGETLQLYSQDP++F
Sbjct: 9   IVISFFISLAFMCYVHYTSRQRRKLHGYGHEKAVRLPPGSMGWPYIGETLQLYSQDPNVF 68

Query: 68  FAAKQKRYGEIFKTHILGCPCVMLASPEAARFVLVTQAHLFKPTYPKSKERLIGPSALFY 127
           FA+KQKRYGEIFKTHILGCPCVMLASPEAARFVLVTQAHLFKPTYP+SKER+IGPSALF+
Sbjct: 69  FASKQKRYGEIFKTHILGCPCVMLASPEAARFVLVTQAHLFKPTYPRSKERMIGPSALFF 128

Query: 128 HQGDYHCRLRKLVQSSLSLDVIRSLVPDIDSVTTSALHSWTGAHLMNTFHEMKKISFEVG 187
           +QGDYH RLRKLVQ  L  D +R+LVPD+++   S L SW G ++ +TFH MK++SF+VG
Sbjct: 129 NQGDYHLRLRKLVQGPLGPDALRALVPDVEAAVRSTLASWDG-NVSSTFHAMKRLSFDVG 188

Query: 188 ILAIF-GHLEAAYKEDLKKNYSILDAGYNSFPTNIPGTPYKKALSARKRLSKILGDIIHE 247
           I+ IF G L+   K +L++NY+I++ GYNSFP + PGT Y KA+ AR+RL  +L DI+ E
Sbjct: 189 IVTIFGGRLDERRKAELRQNYAIVEKGYNSFPNSFPGTLYYKAIQARRRLHGVLSDIMRE 248

Query: 248 RREKKVGKKDLLGCFLNS-IDEEGEAISDAQMEDNIIGVLFAAQDTTASVMTWVVKYLYD 307
           RR +     DLLGC + S   ++G  ++D Q+ DNIIGVLFAAQDTTASV+TW+VKYL+D
Sbjct: 249 RRARGEPGSDLLGCLMQSRAGDDGALLTDEQVADNIIGVLFAAQDTTASVLTWIVKYLHD 308

Query: 308 QPKLLQTLKKEQQEILEFNYEKNQPLNWVQTRNMPMSYKVVLESLRMASIISFTFREAVA 367
            PKLL+ ++ EQ  I   N     PL W QTR+M +++KV+LESLRMASIISFTFREAVA
Sbjct: 309 HPKLLEAVRAEQAAIRAANDGGRLPLTWAQTRSMALTHKVILESLRMASIISFTFREAVA 368

Query: 368 DVEYKGYLIPKGWKVMPLFRNIHHSPEYFADPHKFDPSRFEGKYGRFKFQVAPKPNTFMP 427
           DVEYKG+LIPKGWKVMPLFRNIHH+P+YF DP KFDPSR         F+V+P+PNTFMP
Sbjct: 369 DVEYKGFLIPKGWKVMPLFRNIHHNPDYFQDPQKFDPSR---------FKVSPRPNTFMP 428

Query: 428 FGSGVHTCPGNELAKLEILILIHHLVTKFRWELVGSQNGVQHSPFPVPSQGLPARFW 475
           FG+GVH CPGNELAKLE+L+LIHHLVT +RWE+VGS + V++SPFPVP  GL A+ W
Sbjct: 429 FGNGVHACPGNELAKLEMLVLIHHLVTGYRWEIVGSSDEVEYSPFPVPKHGLLAKLW 475

BLAST of CmoCh01G018990 vs. ExPASy Swiss-Prot
Match: Q09J78 (Abscisic acid 8'-hydroxylase 2 OS=Oryza sativa subsp. indica OX=39946 GN=CYP707A6 PE=2 SV=1)

HSP 1 Score: 607.1 bits (1564), Expect = 1.8e-172
Identity = 295/487 (60.57%), Postives = 380/487 (78.03%), Query Frame = 0

Query: 4   IFASLLLF----FLIVLSYIYLNRN-KRAGIRDGAK-----LPPGSLGWPYIGETLQLYS 63
           + A++L F    FL++ + +   R+  + G RD  K     LPPGS+GWPY+GETLQLYS
Sbjct: 10  VTAAVLCFVVPAFLLLCTSVQRRRDVGQGGGRDWQKKKKLRLPPGSMGWPYVGETLQLYS 69

Query: 64  QDPDLFFAAKQKRYGEIFKTHILGCPCVMLASPEAARFVLVTQAHLFKPTYPKSKERLIG 123
           QDP++FFA+KQKRYGEIFKT++LGCPCVMLASPEAARFVLV+QA LFKPTYP SKER+IG
Sbjct: 70  QDPNVFFASKQKRYGEIFKTNLLGCPCVMLASPEAARFVLVSQARLFKPTYPPSKERMIG 129

Query: 124 PSALFYHQGDYHCRLRKLVQSSLSLDVIRSLVPDIDSVTTSALHSWTGAHLMNTFHEMKK 183
           PSALF+HQG+YH RLR+LVQ++L+ D +R+LVPD+D+   + L +W+G H+ +TFH MKK
Sbjct: 130 PSALFFHQGEYHLRLRRLVQAALAPDSLRALVPDVDAAVAATLAAWSGGHVASTFHAMKK 189

Query: 184 ISFEVGILAIF-GHLEAAYKEDLKKNYSILDAGYNSFPTNIPGTPYKKALSARKRLSKIL 243
           +SF+VG++ IF G L   ++E+L+ NYS+++ GYN FP   PGT Y KA+ ARKRL  IL
Sbjct: 190 LSFDVGVVTIFGGRLGRRHREELRTNYSVVERGYNCFPNRFPGTLYHKAIQARKRLRAIL 249

Query: 244 GDIIHERREKKVGKKDLLGCFLNSIDEEGEA-----ISDAQMEDNIIGVLFAAQDTTASV 303
            +I+ ERR +  G  DLLG  + S D +G A     ++D Q+ DN++GVLFAAQDTTASV
Sbjct: 250 SEIVAERRARGGGGDDLLGGLMRSRD-DGTAGAVALLTDDQIADNVVGVLFAAQDTTASV 309

Query: 304 MTWVVKYLYDQPKLLQTLKKEQQEILEFNYEKNQPLNWVQTRNMPMSYKVVLESLRMASI 363
           +TW++KYL+D PKLL+ +K EQ  I   N    +PL W QTR+M ++++V+LESLRMASI
Sbjct: 310 LTWILKYLHDSPKLLEAVKAEQMAIYVANEGGKRPLTWTQTRSMTLTHQVILESLRMASI 369

Query: 364 ISFTFREAVADVEYKGYLIPKGWKVMPLFRNIHHSPEYFADPHKFDPSRFEGKYGRFKFQ 423
           ISFTFREAVADVEYKG+LIPKGWKVMPLFRNIHH+P+YF DP KFDPSR         F+
Sbjct: 370 ISFTFREAVADVEYKGFLIPKGWKVMPLFRNIHHNPDYFQDPQKFDPSR---------FK 429

Query: 424 VAPKPNTFMPFGSGVHTCPGNELAKLEILILIHHLVTKFRWELVGSQNGVQHSPFPVPSQ 475
           VAP+P+TF+PFGSGVH CPGNELAKLE+L+L+H LVT +RWE+VG+ + V++SPFPVP  
Sbjct: 430 VAPRPSTFLPFGSGVHACPGNELAKLEMLVLVHRLVTAYRWEIVGASDEVEYSPFPVPRG 486

BLAST of CmoCh01G018990 vs. ExPASy TrEMBL
Match: A0A6J1FQ85 (abscisic acid 8'-hydroxylase 4-like OS=Cucurbita moschata OX=3662 GN=LOC111445975 PE=3 SV=1)

HSP 1 Score: 977.2 bits (2525), Expect = 2.4e-281
Identity = 480/489 (98.16%), Postives = 480/489 (98.16%), Query Frame = 0

Query: 1   MGVIFASLLLFFLIVLSYIYLNRNKRAGIRDGAKLPPGSLGWPYIGETLQLYSQDPDLFF 60
           MGVIFASLLLFFLIVLSYIYLNRNKRAGIRDGAKLPPGSLGWPYIGETLQLYSQDPDLFF
Sbjct: 1   MGVIFASLLLFFLIVLSYIYLNRNKRAGIRDGAKLPPGSLGWPYIGETLQLYSQDPDLFF 60

Query: 61  AAKQKRYGEIFKTHILGCPCVMLASPEAARFVLVTQAHLFKPTYPKSKERLIGPSALFYH 120
           AAKQKRYGEIFKTHILGCPCVMLASPEAARFVLVTQAHLFKPTYPKSKERLIGPSALFYH
Sbjct: 61  AAKQKRYGEIFKTHILGCPCVMLASPEAARFVLVTQAHLFKPTYPKSKERLIGPSALFYH 120

Query: 121 QGDYHCRLRKLVQSSLSLDVIRSLVPDIDSVTTSALHSWTGAHLMNTFHEMKKISFEVGI 180
           QGDYHCRLRKLVQSSLSLDVIRSLVPDIDSVTTSALHSWTGAHLMNTFHEMKKISFEVGI
Sbjct: 121 QGDYHCRLRKLVQSSLSLDVIRSLVPDIDSVTTSALHSWTGAHLMNTFHEMKKISFEVGI 180

Query: 181 LAIFGHLEAAYKEDLKKNYSILDAGYNSFPTNIPGTPYKKALSARKRLSKILGDIIHERR 240
           LAIFGHLEAAYKEDLKKNYSILDAGYNSFPTNIPGTPYKKALSARKRLSKILGDIIHERR
Sbjct: 181 LAIFGHLEAAYKEDLKKNYSILDAGYNSFPTNIPGTPYKKALSARKRLSKILGDIIHERR 240

Query: 241 EKKVGKKDLLGCFLNSIDEEGEAISDAQMEDNIIGVLFAAQDTTASVMTWVVKYLYDQPK 300
           EKKVGKKDLLGCFLNSIDEEGEAISDAQMEDNIIGVLFAAQDTTASVMTWVVKYLYDQPK
Sbjct: 241 EKKVGKKDLLGCFLNSIDEEGEAISDAQMEDNIIGVLFAAQDTTASVMTWVVKYLYDQPK 300

Query: 301 LLQTLKKEQQEILEFNYEKNQPLNWVQTRNMPMSYKVVLESLRMASIISFTFREAVADVE 360
           LLQTLKKEQQEILEFNYEKNQPLNWVQTRNMPMSYKVVLESLRMASIISFTFREAVADVE
Sbjct: 301 LLQTLKKEQQEILEFNYEKNQPLNWVQTRNMPMSYKVVLESLRMASIISFTFREAVADVE 360

Query: 361 YKGYLIPKGWKVMPLFRNIHHSPEYFADPHKFDPSRFEGKYGRFKFQVAPKPNTFMPFGS 420
           YKGYLIPKGWKVMPLFRNIHHSPEYFADPHKFDPSRFE         VAPKPNTFMPFGS
Sbjct: 361 YKGYLIPKGWKVMPLFRNIHHSPEYFADPHKFDPSRFE---------VAPKPNTFMPFGS 420

Query: 421 GVHTCPGNELAKLEILILIHHLVTKFRWELVGSQNGVQHSPFPVPSQGLPARFWNIAHHN 480
           GVHTCPGNELAKLEILILIHHLVTKFRWELVGSQNGVQHSPFPVPSQGLPARFWNIAHHN
Sbjct: 421 GVHTCPGNELAKLEILILIHHLVTKFRWELVGSQNGVQHSPFPVPSQGLPARFWNIAHHN 480

Query: 481 TVHTKMDPN 490
           TVHTKMDPN
Sbjct: 481 TVHTKMDPN 480

BLAST of CmoCh01G018990 vs. ExPASy TrEMBL
Match: A0A6J1IYR9 (abscisic acid 8'-hydroxylase 4-like OS=Cucurbita maxima OX=3661 GN=LOC111481123 PE=3 SV=1)

HSP 1 Score: 939.1 bits (2426), Expect = 7.4e-270
Identity = 463/489 (94.68%), Postives = 469/489 (95.91%), Query Frame = 0

Query: 1   MGVIFASLLLFFLIVLSYIYLNRNKRAGIRDGAKLPPGSLGWPYIGETLQLYSQDPDLFF 60
           MGVIFASLLLFFLIVLSYIYLNR KRAGIRDGAKLPPGSLGWPYIGETLQLYSQDPDLFF
Sbjct: 1   MGVIFASLLLFFLIVLSYIYLNRTKRAGIRDGAKLPPGSLGWPYIGETLQLYSQDPDLFF 60

Query: 61  AAKQKRYGEIFKTHILGCPCVMLASPEAARFVLVTQAHLFKPTYPKSKERLIGPSALFYH 120
           AAKQKRYGEIFKTHILGCPCVMLASPEAARFVLVTQAHLFKPTYPKSKERLIGPSALFYH
Sbjct: 61  AAKQKRYGEIFKTHILGCPCVMLASPEAARFVLVTQAHLFKPTYPKSKERLIGPSALFYH 120

Query: 121 QGDYHCRLRKLVQSSLSLDVIRSLVPDIDSVTTSALHSWTGAHLMNTFHEMKKISFEVGI 180
           QGDYHCRLRKLVQ+SLSLDVIRSLVPDIDSVTTSALHSWTG  L+NTFHEMKKISFEVGI
Sbjct: 121 QGDYHCRLRKLVQNSLSLDVIRSLVPDIDSVTTSALHSWTGTQLINTFHEMKKISFEVGI 180

Query: 181 LAIFGHLEAAYKEDLKKNYSILDAGYNSFPTNIPGTPYKKALSARKRLSKILGDIIHERR 240
           LAIFGHLEAAYKEDLKKNYSILDAGYNSFPTNIPGTPYKKALSARKRLSKILGDIIHERR
Sbjct: 181 LAIFGHLEAAYKEDLKKNYSILDAGYNSFPTNIPGTPYKKALSARKRLSKILGDIIHERR 240

Query: 241 EKKVGKKDLLGCFLNSIDEEGEAISDAQMEDNIIGVLFAAQDTTASVMTWVVKYLYDQPK 300
           EKKVGKKDLLG FLNSIDEEGEA+SDAQ+EDNIIGVLFAAQDTTASVMTWVVKYLYDQPK
Sbjct: 241 EKKVGKKDLLGWFLNSIDEEGEAMSDAQIEDNIIGVLFAAQDTTASVMTWVVKYLYDQPK 300

Query: 301 LLQTLKKEQQEILEFNYEKNQPLNWVQTRNMPMSYKVVLESLRMASIISFTFREAVADVE 360
           LLQTLKKEQ+EILEFN +KNQ LNWVQTRNMPMSYKVVLESLRMASIISFTFREAVADVE
Sbjct: 301 LLQTLKKEQEEILEFNCQKNQQLNWVQTRNMPMSYKVVLESLRMASIISFTFREAVADVE 360

Query: 361 YKGYLIPKGWKVMPLFRNIHHSPEYFADPHKFDPSRFEGKYGRFKFQVAPKPNTFMPFGS 420
           YKGYLIPKGWKVMPLFRNIH SPEYFADPHKFDPSRFE         VAPKPNTFMPFGS
Sbjct: 361 YKGYLIPKGWKVMPLFRNIHRSPEYFADPHKFDPSRFE---------VAPKPNTFMPFGS 420

Query: 421 GVHTCPGNELAKLEILILIHHLVTKFRWELVGSQNGVQHSPFPVPSQGLPARFWNIAHHN 480
           GVH CPGNELAKLEILILIHHLVTKFRWELVGSQNGVQHSPFPVP QGLPARFWNIAH  
Sbjct: 421 GVHACPGNELAKLEILILIHHLVTKFRWELVGSQNGVQHSPFPVPFQGLPARFWNIAHQK 480

Query: 481 TVHTKMDPN 490
           TVHTKMDPN
Sbjct: 481 TVHTKMDPN 480

BLAST of CmoCh01G018990 vs. ExPASy TrEMBL
Match: A0A1S3BTH6 (abscisic acid 8'-hydroxylase 4-like OS=Cucumis melo OX=3656 GN=LOC103493539 PE=3 SV=1)

HSP 1 Score: 825.9 bits (2132), Expect = 9.1e-236
Identity = 405/479 (84.55%), Postives = 434/479 (90.61%), Query Frame = 0

Query: 1   MGVIFASLLLFFLIVLSYIYLNRNKRAGIRDGAKLPPGSLGWPYIGETLQLYSQDPDLFF 60
           MGVIF S+L+FFL +L YI L R+K+A IRDG KLPPGSLGWPYIGETLQLYSQ PD FF
Sbjct: 1   MGVIFLSILIFFLSLLWYISLKRDKKAAIRDGTKLPPGSLGWPYIGETLQLYSQHPDFFF 60

Query: 61  AAKQKRYGEIFKTHILGCPCVMLASPEAARFVLVTQAHLFKPTYPKSKERLIGPSALFYH 120
           AAKQKRYGEIFKTHILGCPCVMLASPEAARFVLVTQAHLFKPTYPKSKE LIGPSALF+H
Sbjct: 61  AAKQKRYGEIFKTHILGCPCVMLASPEAARFVLVTQAHLFKPTYPKSKELLIGPSALFFH 120

Query: 121 QGDYHCRLRKLVQSSLSLDVIRSLVPDIDSVTTSALHSWTGAHLMNTFHEMKKISFEVGI 180
           QGDYHCRLRKLVQ+SLSLD IR+LVPDIDSV  SAL SW  + ++NTFHEMKKISFEVGI
Sbjct: 121 QGDYHCRLRKLVQNSLSLDSIRTLVPDIDSVAASALDSWATSEVINTFHEMKKISFEVGI 180

Query: 181 LAIFGHLEAAYKEDLKKNYSILDAGYNSFPTNIPGTPYKKALSARKRLSKILGDIIHERR 240
           L IFGHLEAAYK+DLKKNYSILDAGYNSFPTNIPGTPYKKALSARKRLSKILGDIIHER 
Sbjct: 181 LTIFGHLEAAYKDDLKKNYSILDAGYNSFPTNIPGTPYKKALSARKRLSKILGDIIHERG 240

Query: 241 EKKVGKKDLLGCFLNSIDEEGEA-ISDAQMEDNIIGVLFAAQDTTASVMTWVVKYLYDQP 300
           E+++ +KDLLG  LNSID+E E  ISD+Q+ DNIIGVLFAAQDTTASVMTW+VKYL+DQP
Sbjct: 241 ERRLREKDLLGSLLNSIDDECEVKISDSQIADNIIGVLFAAQDTTASVMTWIVKYLHDQP 300

Query: 301 KLLQTLKKEQQEILEFNYEKNQPLNWVQTRNMPMSYKVVLESLRMASIISFTFREAVADV 360
           KLLQ+LK EQ+EIL+ N E NQ LNWVQTRNMP++YK+VLESLRMASIIS+TFREAVADV
Sbjct: 301 KLLQSLKAEQEEILKLNSENNQGLNWVQTRNMPITYKMVLESLRMASIISYTFREAVADV 360

Query: 361 EYKGYLIPKGWKVMPLFRNIHHSPEYFADPHKFDPSRFEGKYGRFKFQVAPKPNTFMPFG 420
           EYKGYLIPKGWKVMPLFRNIHHSPEYF DPH FDPSRFE         VAPKPNTFMPFG
Sbjct: 361 EYKGYLIPKGWKVMPLFRNIHHSPEYFPDPHNFDPSRFE---------VAPKPNTFMPFG 420

Query: 421 SGVHTCPGNELAKLEILILIHHLVTKFRWELVGSQNGVQHSPFPVPSQGLPARFWNIAH 479
           SGVH CPGNELAKLEILI+IHHLVTKFRWELVGSQNGVQHSPFPVP QGLPARFW IAH
Sbjct: 421 SGVHACPGNELAKLEILIMIHHLVTKFRWELVGSQNGVQHSPFPVPFQGLPARFWKIAH 470

BLAST of CmoCh01G018990 vs. ExPASy TrEMBL
Match: A0A0A0L3K1 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G639800 PE=3 SV=1)

HSP 1 Score: 815.5 bits (2105), Expect = 1.2e-232
Identity = 399/479 (83.30%), Postives = 431/479 (89.98%), Query Frame = 0

Query: 1   MGVIFASLLLFFLIVLSYIYLNRNKRAGIRDGAKLPPGSLGWPYIGETLQLYSQDPDLFF 60
           MGV+F S+L+FFL +L YI L R+K+A IRDG KLPPGSLGWPYIGETLQLYSQ PD FF
Sbjct: 1   MGVVFLSILIFFLTLLWYISLKRDKKAAIRDGTKLPPGSLGWPYIGETLQLYSQHPDFFF 60

Query: 61  AAKQKRYGEIFKTHILGCPCVMLASPEAARFVLVTQAHLFKPTYPKSKERLIGPSALFYH 120
           AAKQKRYGEIFKTHILGCPCVMLASPEAARFVLVTQAHLFKPTYPKSKE LIGPSALF+H
Sbjct: 61  AAKQKRYGEIFKTHILGCPCVMLASPEAARFVLVTQAHLFKPTYPKSKELLIGPSALFFH 120

Query: 121 QGDYHCRLRKLVQSSLSLDVIRSLVPDIDSVTTSALHSWTGAHLMNTFHEMKKISFEVGI 180
           QGDYHCRLRKLVQSSLSLD IR+LVPDIDSV  SAL SW  + ++NTFHEMKKISFEVGI
Sbjct: 121 QGDYHCRLRKLVQSSLSLDSIRTLVPDIDSVAASALDSWATSGVINTFHEMKKISFEVGI 180

Query: 181 LAIFGHLEAAYKEDLKKNYSILDAGYNSFPTNIPGTPYKKALSARKRLSKILGDIIHERR 240
           L IFGHLEAAYK+DLKKNYSIL+AGYNSFPTNIPGTPYKKALSARKRL+KILGDIIHER 
Sbjct: 181 LTIFGHLEAAYKDDLKKNYSILEAGYNSFPTNIPGTPYKKALSARKRLNKILGDIIHERG 240

Query: 241 EKKVGKKDLLGCFLNSIDEEGEA-ISDAQMEDNIIGVLFAAQDTTASVMTWVVKYLYDQP 300
           E+++ +KDLLG  LNSID+E E  ISD+Q+ DNIIG LFAAQDTTASVMTW+VKYL+D+P
Sbjct: 241 ERRLREKDLLGSLLNSIDDECEVKISDSQIADNIIGALFAAQDTTASVMTWIVKYLHDRP 300

Query: 301 KLLQTLKKEQQEILEFNYEKNQPLNWVQTRNMPMSYKVVLESLRMASIISFTFREAVADV 360
           KL Q++K EQ+EIL+ N E NQ LNWVQTRNMP++YK+VLESLRMASIISFTFREAVADV
Sbjct: 301 KLRQSIKAEQEEILKLNCENNQGLNWVQTRNMPITYKMVLESLRMASIISFTFREAVADV 360

Query: 361 EYKGYLIPKGWKVMPLFRNIHHSPEYFADPHKFDPSRFEGKYGRFKFQVAPKPNTFMPFG 420
           EYKGYLIPKGWKVMPLFRNIHHSPEYF DPH FDPSRFE         VAPKPNTFMPFG
Sbjct: 361 EYKGYLIPKGWKVMPLFRNIHHSPEYFPDPHNFDPSRFE---------VAPKPNTFMPFG 420

Query: 421 SGVHTCPGNELAKLEILILIHHLVTKFRWELVGSQNGVQHSPFPVPSQGLPARFWNIAH 479
           SGVH CPGNELAKLEILI+IHHLVTKFRWELVGSQN VQHSPFPVP QGLPARFW IAH
Sbjct: 421 SGVHACPGNELAKLEILIMIHHLVTKFRWELVGSQNRVQHSPFPVPFQGLPARFWKIAH 470

BLAST of CmoCh01G018990 vs. ExPASy TrEMBL
Match: A0A5A7V8C1 (Abscisic acid 8'-hydroxylase 4-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold255G00840 PE=3 SV=1)

HSP 1 Score: 810.1 bits (2091), Expect = 5.2e-231
Identity = 395/465 (84.95%), Postives = 422/465 (90.75%), Query Frame = 0

Query: 15  VLSYIYLNRNKRAGIRDGAKLPPGSLGWPYIGETLQLYSQDPDLFFAAKQKRYGEIFKTH 74
           +L YI L R+K+A IRDG KLPPGSLGWPYIGETLQLYSQ PD FFAAKQKRYGEIFKTH
Sbjct: 31  LLWYISLKRDKKAAIRDGTKLPPGSLGWPYIGETLQLYSQHPDFFFAAKQKRYGEIFKTH 90

Query: 75  ILGCPCVMLASPEAARFVLVTQAHLFKPTYPKSKERLIGPSALFYHQGDYHCRLRKLVQS 134
           ILGCPCVMLASPEAARFVLVTQAHLFKPTYPKSKE LIGPSALF+HQGDYHCRLRKLVQ+
Sbjct: 91  ILGCPCVMLASPEAARFVLVTQAHLFKPTYPKSKELLIGPSALFFHQGDYHCRLRKLVQN 150

Query: 135 SLSLDVIRSLVPDIDSVTTSALHSWTGAHLMNTFHEMKKISFEVGILAIFGHLEAAYKED 194
           SLSLD IR+LVPDIDSV  SAL SW  + ++NTFHEMKKISFEVGIL IFGHLEAAYK+D
Sbjct: 151 SLSLDSIRTLVPDIDSVAASALDSWATSEVINTFHEMKKISFEVGILTIFGHLEAAYKDD 210

Query: 195 LKKNYSILDAGYNSFPTNIPGTPYKKALSARKRLSKILGDIIHERREKKVGKKDLLGCFL 254
           LKKNYSILDAGYNSFPTNIPGTPYKKALSARKRLSKILGDIIHER E+++ +KDLLG  L
Sbjct: 211 LKKNYSILDAGYNSFPTNIPGTPYKKALSARKRLSKILGDIIHERGERRLREKDLLGSLL 270

Query: 255 NSIDEEGEA-ISDAQMEDNIIGVLFAAQDTTASVMTWVVKYLYDQPKLLQTLKKEQQEIL 314
           NSID+E E  ISD+Q+ DNIIGVLFAAQDTTASVMTW+VKYL+DQPKLLQ+LK EQ+EIL
Sbjct: 271 NSIDDECEVKISDSQIADNIIGVLFAAQDTTASVMTWIVKYLHDQPKLLQSLKAEQEEIL 330

Query: 315 EFNYEKNQPLNWVQTRNMPMSYKVVLESLRMASIISFTFREAVADVEYKGYLIPKGWKVM 374
           + N E NQ LNWVQTRNMP++YK+VLESLRMASIIS+TFREAVADVEYKGYLIPKGWKVM
Sbjct: 331 KLNSENNQGLNWVQTRNMPITYKMVLESLRMASIISYTFREAVADVEYKGYLIPKGWKVM 390

Query: 375 PLFRNIHHSPEYFADPHKFDPSRFEGKYGRFKFQVAPKPNTFMPFGSGVHTCPGNELAKL 434
           PLFRNIHHSPEYF DPH FDPSRFE         VAPKPNTFMPFGSGVH CPGNELAKL
Sbjct: 391 PLFRNIHHSPEYFPDPHNFDPSRFE---------VAPKPNTFMPFGSGVHACPGNELAKL 450

Query: 435 EILILIHHLVTKFRWELVGSQNGVQHSPFPVPSQGLPARFWNIAH 479
           EILI+IHHLVTKFRWELVGSQNGVQHSPFPVP QGLPARFW IAH
Sbjct: 451 EILIMIHHLVTKFRWELVGSQNGVQHSPFPVPFQGLPARFWKIAH 486

BLAST of CmoCh01G018990 vs. NCBI nr
Match: XP_022940325.1 (abscisic acid 8'-hydroxylase 4-like [Cucurbita moschata])

HSP 1 Score: 977.2 bits (2525), Expect = 5.0e-281
Identity = 480/489 (98.16%), Postives = 480/489 (98.16%), Query Frame = 0

Query: 1   MGVIFASLLLFFLIVLSYIYLNRNKRAGIRDGAKLPPGSLGWPYIGETLQLYSQDPDLFF 60
           MGVIFASLLLFFLIVLSYIYLNRNKRAGIRDGAKLPPGSLGWPYIGETLQLYSQDPDLFF
Sbjct: 1   MGVIFASLLLFFLIVLSYIYLNRNKRAGIRDGAKLPPGSLGWPYIGETLQLYSQDPDLFF 60

Query: 61  AAKQKRYGEIFKTHILGCPCVMLASPEAARFVLVTQAHLFKPTYPKSKERLIGPSALFYH 120
           AAKQKRYGEIFKTHILGCPCVMLASPEAARFVLVTQAHLFKPTYPKSKERLIGPSALFYH
Sbjct: 61  AAKQKRYGEIFKTHILGCPCVMLASPEAARFVLVTQAHLFKPTYPKSKERLIGPSALFYH 120

Query: 121 QGDYHCRLRKLVQSSLSLDVIRSLVPDIDSVTTSALHSWTGAHLMNTFHEMKKISFEVGI 180
           QGDYHCRLRKLVQSSLSLDVIRSLVPDIDSVTTSALHSWTGAHLMNTFHEMKKISFEVGI
Sbjct: 121 QGDYHCRLRKLVQSSLSLDVIRSLVPDIDSVTTSALHSWTGAHLMNTFHEMKKISFEVGI 180

Query: 181 LAIFGHLEAAYKEDLKKNYSILDAGYNSFPTNIPGTPYKKALSARKRLSKILGDIIHERR 240
           LAIFGHLEAAYKEDLKKNYSILDAGYNSFPTNIPGTPYKKALSARKRLSKILGDIIHERR
Sbjct: 181 LAIFGHLEAAYKEDLKKNYSILDAGYNSFPTNIPGTPYKKALSARKRLSKILGDIIHERR 240

Query: 241 EKKVGKKDLLGCFLNSIDEEGEAISDAQMEDNIIGVLFAAQDTTASVMTWVVKYLYDQPK 300
           EKKVGKKDLLGCFLNSIDEEGEAISDAQMEDNIIGVLFAAQDTTASVMTWVVKYLYDQPK
Sbjct: 241 EKKVGKKDLLGCFLNSIDEEGEAISDAQMEDNIIGVLFAAQDTTASVMTWVVKYLYDQPK 300

Query: 301 LLQTLKKEQQEILEFNYEKNQPLNWVQTRNMPMSYKVVLESLRMASIISFTFREAVADVE 360
           LLQTLKKEQQEILEFNYEKNQPLNWVQTRNMPMSYKVVLESLRMASIISFTFREAVADVE
Sbjct: 301 LLQTLKKEQQEILEFNYEKNQPLNWVQTRNMPMSYKVVLESLRMASIISFTFREAVADVE 360

Query: 361 YKGYLIPKGWKVMPLFRNIHHSPEYFADPHKFDPSRFEGKYGRFKFQVAPKPNTFMPFGS 420
           YKGYLIPKGWKVMPLFRNIHHSPEYFADPHKFDPSRFE         VAPKPNTFMPFGS
Sbjct: 361 YKGYLIPKGWKVMPLFRNIHHSPEYFADPHKFDPSRFE---------VAPKPNTFMPFGS 420

Query: 421 GVHTCPGNELAKLEILILIHHLVTKFRWELVGSQNGVQHSPFPVPSQGLPARFWNIAHHN 480
           GVHTCPGNELAKLEILILIHHLVTKFRWELVGSQNGVQHSPFPVPSQGLPARFWNIAHHN
Sbjct: 421 GVHTCPGNELAKLEILILIHHLVTKFRWELVGSQNGVQHSPFPVPSQGLPARFWNIAHHN 480

Query: 481 TVHTKMDPN 490
           TVHTKMDPN
Sbjct: 481 TVHTKMDPN 480

BLAST of CmoCh01G018990 vs. NCBI nr
Match: KAG7037803.1 (Abscisic acid 8'-hydroxylase 4, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 973.0 bits (2514), Expect = 9.5e-280
Identity = 477/489 (97.55%), Postives = 480/489 (98.16%), Query Frame = 0

Query: 1   MGVIFASLLLFFLIVLSYIYLNRNKRAGIRDGAKLPPGSLGWPYIGETLQLYSQDPDLFF 60
           MGVIFASLLLFFLIVLSYIYLNRNKRAGIRDGAKLPPGSLGWPYIGETLQLYSQDPDLFF
Sbjct: 1   MGVIFASLLLFFLIVLSYIYLNRNKRAGIRDGAKLPPGSLGWPYIGETLQLYSQDPDLFF 60

Query: 61  AAKQKRYGEIFKTHILGCPCVMLASPEAARFVLVTQAHLFKPTYPKSKERLIGPSALFYH 120
           AAKQKRYGEIFKTHILGCPCVMLASPEAARFVLVTQAHLFKPTYPKSKERLIGPSALFYH
Sbjct: 61  AAKQKRYGEIFKTHILGCPCVMLASPEAARFVLVTQAHLFKPTYPKSKERLIGPSALFYH 120

Query: 121 QGDYHCRLRKLVQSSLSLDVIRSLVPDIDSVTTSALHSWTGAHLMNTFHEMKKISFEVGI 180
           QGDYHCRLRKLVQ+SLSLDVIRSLVPDIDSVTTSALHSWTGAHL+NTFHEMKKISFEVGI
Sbjct: 121 QGDYHCRLRKLVQNSLSLDVIRSLVPDIDSVTTSALHSWTGAHLINTFHEMKKISFEVGI 180

Query: 181 LAIFGHLEAAYKEDLKKNYSILDAGYNSFPTNIPGTPYKKALSARKRLSKILGDIIHERR 240
           LAIFGHLEAAYKEDLKKNYSILDAGYNSFPTNIPGTPYKKALSARKRLSKILGDIIHERR
Sbjct: 181 LAIFGHLEAAYKEDLKKNYSILDAGYNSFPTNIPGTPYKKALSARKRLSKILGDIIHERR 240

Query: 241 EKKVGKKDLLGCFLNSIDEEGEAISDAQMEDNIIGVLFAAQDTTASVMTWVVKYLYDQPK 300
           EKKVGKKDLLGCFLNSIDEEGEAISDAQ+EDNIIGVLFAAQDTTASVMTWVVKYLYDQPK
Sbjct: 241 EKKVGKKDLLGCFLNSIDEEGEAISDAQIEDNIIGVLFAAQDTTASVMTWVVKYLYDQPK 300

Query: 301 LLQTLKKEQQEILEFNYEKNQPLNWVQTRNMPMSYKVVLESLRMASIISFTFREAVADVE 360
           LLQTLKKEQQEILEFNYEKNQPLNWVQTRNMPMSYKVVLESLRMASIISFTFREAVADVE
Sbjct: 301 LLQTLKKEQQEILEFNYEKNQPLNWVQTRNMPMSYKVVLESLRMASIISFTFREAVADVE 360

Query: 361 YKGYLIPKGWKVMPLFRNIHHSPEYFADPHKFDPSRFEGKYGRFKFQVAPKPNTFMPFGS 420
           YKGYLIPKGWKVMPLFRNIHHSPEYFADPHKFDPSRFE         VAPKPNTFMPFGS
Sbjct: 361 YKGYLIPKGWKVMPLFRNIHHSPEYFADPHKFDPSRFE---------VAPKPNTFMPFGS 420

Query: 421 GVHTCPGNELAKLEILILIHHLVTKFRWELVGSQNGVQHSPFPVPSQGLPARFWNIAHHN 480
           GVHTCPGNELAKLEILILIHHLVTKFRWELVGSQNGVQHSPFPVPSQGLPARFWNIAHHN
Sbjct: 421 GVHTCPGNELAKLEILILIHHLVTKFRWELVGSQNGVQHSPFPVPSQGLPARFWNIAHHN 480

Query: 481 TVHTKMDPN 490
           TVHTKMDPN
Sbjct: 481 TVHTKMDPN 480

BLAST of CmoCh01G018990 vs. NCBI nr
Match: XP_023525021.1 (abscisic acid 8'-hydroxylase 4-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 961.4 bits (2484), Expect = 2.9e-276
Identity = 471/489 (96.32%), Postives = 476/489 (97.34%), Query Frame = 0

Query: 1   MGVIFASLLLFFLIVLSYIYLNRNKRAGIRDGAKLPPGSLGWPYIGETLQLYSQDPDLFF 60
           MGVIFASLLLFFLIVLSYIYLNRNKRAGIRDGAKLPPGSLGWPYIGETLQLYSQDPDLFF
Sbjct: 1   MGVIFASLLLFFLIVLSYIYLNRNKRAGIRDGAKLPPGSLGWPYIGETLQLYSQDPDLFF 60

Query: 61  AAKQKRYGEIFKTHILGCPCVMLASPEAARFVLVTQAHLFKPTYPKSKERLIGPSALFYH 120
           AAKQKRYGEIFKTHILGCPCVMLASPEAARFVLVTQAHLF PTYPKSKERLIGPSALFYH
Sbjct: 61  AAKQKRYGEIFKTHILGCPCVMLASPEAARFVLVTQAHLFNPTYPKSKERLIGPSALFYH 120

Query: 121 QGDYHCRLRKLVQSSLSLDVIRSLVPDIDSVTTSALHSWTGAHLMNTFHEMKKISFEVGI 180
           QGDYHCRLRKLVQ+SLSLDVIRSLVPDIDSVTTSALHSWTGAHL+NTFHEMKKISFEVGI
Sbjct: 121 QGDYHCRLRKLVQNSLSLDVIRSLVPDIDSVTTSALHSWTGAHLINTFHEMKKISFEVGI 180

Query: 181 LAIFGHLEAAYKEDLKKNYSILDAGYNSFPTNIPGTPYKKALSARKRLSKILGDIIHERR 240
           LAIFGHLEAAYKEDLKKNYSILDAGYNSFPTN+PGTPYKKALSARKRLSKILGDIIHERR
Sbjct: 181 LAIFGHLEAAYKEDLKKNYSILDAGYNSFPTNVPGTPYKKALSARKRLSKILGDIIHERR 240

Query: 241 EKKVGKKDLLGCFLNSIDEEGEAISDAQMEDNIIGVLFAAQDTTASVMTWVVKYLYDQPK 300
           EKKVGKKDLLGCFLNSIDEEGEAISDAQ+EDNIIGVLFAAQDTTASVMTWVVKYLYDQPK
Sbjct: 241 EKKVGKKDLLGCFLNSIDEEGEAISDAQIEDNIIGVLFAAQDTTASVMTWVVKYLYDQPK 300

Query: 301 LLQTLKKEQQEILEFNYEKNQPLNWVQTRNMPMSYKVVLESLRMASIISFTFREAVADVE 360
           LLQTLKKEQQEILE NYEKNQPLNWVQTRNMPMSYKVVLESLRMASIISFTFREAVADVE
Sbjct: 301 LLQTLKKEQQEILELNYEKNQPLNWVQTRNMPMSYKVVLESLRMASIISFTFREAVADVE 360

Query: 361 YKGYLIPKGWKVMPLFRNIHHSPEYFADPHKFDPSRFEGKYGRFKFQVAPKPNTFMPFGS 420
           YKGYLIPKGWKVMPLFRNIHHS EYFA+PHKFDPSRFE         VAPKPNTFMPFGS
Sbjct: 361 YKGYLIPKGWKVMPLFRNIHHSSEYFAEPHKFDPSRFE---------VAPKPNTFMPFGS 420

Query: 421 GVHTCPGNELAKLEILILIHHLVTKFRWELVGSQNGVQHSPFPVPSQGLPARFWNIAHHN 480
           GVHTCPGNELAKLEILILIHHLVTKFRWELVGSQNGVQHSPFPVP QGLPARFWNIAHHN
Sbjct: 421 GVHTCPGNELAKLEILILIHHLVTKFRWELVGSQNGVQHSPFPVPFQGLPARFWNIAHHN 480

Query: 481 TVHTKMDPN 490
           TVHTKMDPN
Sbjct: 481 TVHTKMDPN 480

BLAST of CmoCh01G018990 vs. NCBI nr
Match: XP_022982231.1 (abscisic acid 8'-hydroxylase 4-like [Cucurbita maxima])

HSP 1 Score: 939.1 bits (2426), Expect = 1.5e-269
Identity = 463/489 (94.68%), Postives = 469/489 (95.91%), Query Frame = 0

Query: 1   MGVIFASLLLFFLIVLSYIYLNRNKRAGIRDGAKLPPGSLGWPYIGETLQLYSQDPDLFF 60
           MGVIFASLLLFFLIVLSYIYLNR KRAGIRDGAKLPPGSLGWPYIGETLQLYSQDPDLFF
Sbjct: 1   MGVIFASLLLFFLIVLSYIYLNRTKRAGIRDGAKLPPGSLGWPYIGETLQLYSQDPDLFF 60

Query: 61  AAKQKRYGEIFKTHILGCPCVMLASPEAARFVLVTQAHLFKPTYPKSKERLIGPSALFYH 120
           AAKQKRYGEIFKTHILGCPCVMLASPEAARFVLVTQAHLFKPTYPKSKERLIGPSALFYH
Sbjct: 61  AAKQKRYGEIFKTHILGCPCVMLASPEAARFVLVTQAHLFKPTYPKSKERLIGPSALFYH 120

Query: 121 QGDYHCRLRKLVQSSLSLDVIRSLVPDIDSVTTSALHSWTGAHLMNTFHEMKKISFEVGI 180
           QGDYHCRLRKLVQ+SLSLDVIRSLVPDIDSVTTSALHSWTG  L+NTFHEMKKISFEVGI
Sbjct: 121 QGDYHCRLRKLVQNSLSLDVIRSLVPDIDSVTTSALHSWTGTQLINTFHEMKKISFEVGI 180

Query: 181 LAIFGHLEAAYKEDLKKNYSILDAGYNSFPTNIPGTPYKKALSARKRLSKILGDIIHERR 240
           LAIFGHLEAAYKEDLKKNYSILDAGYNSFPTNIPGTPYKKALSARKRLSKILGDIIHERR
Sbjct: 181 LAIFGHLEAAYKEDLKKNYSILDAGYNSFPTNIPGTPYKKALSARKRLSKILGDIIHERR 240

Query: 241 EKKVGKKDLLGCFLNSIDEEGEAISDAQMEDNIIGVLFAAQDTTASVMTWVVKYLYDQPK 300
           EKKVGKKDLLG FLNSIDEEGEA+SDAQ+EDNIIGVLFAAQDTTASVMTWVVKYLYDQPK
Sbjct: 241 EKKVGKKDLLGWFLNSIDEEGEAMSDAQIEDNIIGVLFAAQDTTASVMTWVVKYLYDQPK 300

Query: 301 LLQTLKKEQQEILEFNYEKNQPLNWVQTRNMPMSYKVVLESLRMASIISFTFREAVADVE 360
           LLQTLKKEQ+EILEFN +KNQ LNWVQTRNMPMSYKVVLESLRMASIISFTFREAVADVE
Sbjct: 301 LLQTLKKEQEEILEFNCQKNQQLNWVQTRNMPMSYKVVLESLRMASIISFTFREAVADVE 360

Query: 361 YKGYLIPKGWKVMPLFRNIHHSPEYFADPHKFDPSRFEGKYGRFKFQVAPKPNTFMPFGS 420
           YKGYLIPKGWKVMPLFRNIH SPEYFADPHKFDPSRFE         VAPKPNTFMPFGS
Sbjct: 361 YKGYLIPKGWKVMPLFRNIHRSPEYFADPHKFDPSRFE---------VAPKPNTFMPFGS 420

Query: 421 GVHTCPGNELAKLEILILIHHLVTKFRWELVGSQNGVQHSPFPVPSQGLPARFWNIAHHN 480
           GVH CPGNELAKLEILILIHHLVTKFRWELVGSQNGVQHSPFPVP QGLPARFWNIAH  
Sbjct: 421 GVHACPGNELAKLEILILIHHLVTKFRWELVGSQNGVQHSPFPVPFQGLPARFWNIAHQK 480

Query: 481 TVHTKMDPN 490
           TVHTKMDPN
Sbjct: 481 TVHTKMDPN 480

BLAST of CmoCh01G018990 vs. NCBI nr
Match: KAG6608475.1 (Abscisic acid 8'-hydroxylase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 936.0 bits (2418), Expect = 1.3e-268
Identity = 454/469 (96.80%), Postives = 459/469 (97.87%), Query Frame = 0

Query: 21  LNRNKRAGIRDGAKLPPGSLGWPYIGETLQLYSQDPDLFFAAKQKRYGEIFKTHILGCPC 80
           L++NKRAGIRDGAKLPPGSLGWPYIGETLQLYSQDPDLFFAAKQKRYGEIFKTHILGCPC
Sbjct: 9   LDKNKRAGIRDGAKLPPGSLGWPYIGETLQLYSQDPDLFFAAKQKRYGEIFKTHILGCPC 68

Query: 81  VMLASPEAARFVLVTQAHLFKPTYPKSKERLIGPSALFYHQGDYHCRLRKLVQSSLSLDV 140
           VMLASPEAARFVLVTQAHLFKPTYPKSKERLIGPSALFYHQGDYHCRLRKLVQ+SLSLDV
Sbjct: 69  VMLASPEAARFVLVTQAHLFKPTYPKSKERLIGPSALFYHQGDYHCRLRKLVQNSLSLDV 128

Query: 141 IRSLVPDIDSVTTSALHSWTGAHLMNTFHEMKKISFEVGILAIFGHLEAAYKEDLKKNYS 200
           IRSLVPDIDSVTTSALHSWTGAHL+NTFHEMKKISFEVGILAIFGHLEAAYKEDLKKNYS
Sbjct: 129 IRSLVPDIDSVTTSALHSWTGAHLINTFHEMKKISFEVGILAIFGHLEAAYKEDLKKNYS 188

Query: 201 ILDAGYNSFPTNIPGTPYKKALSARKRLSKILGDIIHERREKKVGKKDLLGCFLNSIDEE 260
           ILDAGYNSFPTNIPGTPYKKALSARKRLSKILGDIIHERREKKVGKKDLLGCFLNSIDEE
Sbjct: 189 ILDAGYNSFPTNIPGTPYKKALSARKRLSKILGDIIHERREKKVGKKDLLGCFLNSIDEE 248

Query: 261 GEAISDAQMEDNIIGVLFAAQDTTASVMTWVVKYLYDQPKLLQTLKKEQQEILEFNYEKN 320
           GEAISDAQ+EDNIIGVLFAAQDTTASVMTWVVKYLYDQPKLLQTLKKEQQEILEFNYEKN
Sbjct: 249 GEAISDAQIEDNIIGVLFAAQDTTASVMTWVVKYLYDQPKLLQTLKKEQQEILEFNYEKN 308

Query: 321 QPLNWVQTRNMPMSYKVVLESLRMASIISFTFREAVADVEYKGYLIPKGWKVMPLFRNIH 380
           QPLNWVQTRNMPMSYKVVLESLRMASIISFTFREAVADVEYKGYLIPKGWKVMPLFRNIH
Sbjct: 309 QPLNWVQTRNMPMSYKVVLESLRMASIISFTFREAVADVEYKGYLIPKGWKVMPLFRNIH 368

Query: 381 HSPEYFADPHKFDPSRFEGKYGRFKFQVAPKPNTFMPFGSGVHTCPGNELAKLEILILIH 440
           HSPEYF DPHKFDPSRFE         VAPKPNTFMPFGSGVHTCPGNELAKLEILILIH
Sbjct: 369 HSPEYFTDPHKFDPSRFE---------VAPKPNTFMPFGSGVHTCPGNELAKLEILILIH 428

Query: 441 HLVTKFRWELVGSQNGVQHSPFPVPSQGLPARFWNIAHHNTVHTKMDPN 490
           HLVTKFRWELVGSQNGVQHSPFPVPSQGLPARFWNIAHHNTVHTKMDPN
Sbjct: 429 HLVTKFRWELVGSQNGVQHSPFPVPSQGLPARFWNIAHHNTVHTKMDPN 468

BLAST of CmoCh01G018990 vs. TAIR 10
Match: AT3G19270.1 (cytochrome P450, family 707, subfamily A, polypeptide 4 )

HSP 1 Score: 633.6 bits (1633), Expect = 1.3e-181
Identity = 299/465 (64.30%), Postives = 371/465 (79.78%), Query Frame = 0

Query: 9   LLFFLIVLSYIYLNRNKRAGIRDGAKLPPGSLGWPYIGETLQLYSQDPDLFFAAKQKRYG 68
           +L   ++L  + +++ K+       KLPPGS+GWPY+GETLQLYSQ+P++FF +KQKRYG
Sbjct: 11  ILILCLLLVRVIVSKKKK---NSRGKLPPGSMGWPYLGETLQLYSQNPNVFFTSKQKRYG 70

Query: 69  EIFKTHILGCPCVMLASPEAARFVLVTQAHLFKPTYPKSKERLIGPSALFYHQGDYHCRL 128
           EIFKT ILG PCVMLASPEAARFVLVT AH+FKPTYP+SKE+LIGPSALF+HQGDYH  +
Sbjct: 71  EIFKTRILGYPCVMLASPEAARFVLVTHAHMFKPTYPRSKEKLIGPSALFFHQGDYHSHI 130

Query: 129 RKLVQSSLSLDVIRSLVPDIDSVTTSALHSWTGAHLMNTFHEMKKISFEVGILAIFGHLE 188
           RKLVQSS   + IR L+PDI+ +  S+L SW    +++T+ EMKK +F+VGILAIFGHLE
Sbjct: 131 RKLVQSSFYPETIRKLIPDIEHIALSSLQSWANMPIVSTYQEMKKFAFDVGILAIFGHLE 190

Query: 189 AAYKEDLKKNYSILDAGYNSFPTNIPGTPYKKALSARKRLSKILGDIIHERREKKVGKKD 248
           ++YKE LK NY+I+D GYNSFP ++PGT Y KAL ARK+L  I+ +II ERREK+  + D
Sbjct: 191 SSYKEILKHNYNIVDKGYNSFPMSLPGTSYHKALMARKQLKTIVSEIICERREKRALQTD 250

Query: 249 LLGCFLNSIDEEGEAISDAQMEDNIIGVLFAAQDTTASVMTWVVKYLYDQPKLLQTLKKE 308
            LG  LN  +E+G  ++  Q+ DNIIGVLFAAQDTTAS +TW++KYL+D  KLL+ +K E
Sbjct: 251 FLGHLLNFKNEKGRVLTQEQIADNIIGVLFAAQDTTASCLTWILKYLHDDQKLLEAVKAE 310

Query: 309 QQEILEFNYEKNQPLNWVQTRNMPMSYKVVLESLRMASIISFTFREAVADVEYKGYLIPK 368
           Q+ I E N  + +PL W QTRNMP+++KV++ESLRMASIISFTFREAV DVEYKGYLIPK
Sbjct: 311 QKAIYEENSREKKPLTWRQTRNMPLTHKVIVESLRMASIISFTFREAVVDVEYKGYLIPK 370

Query: 369 GWKVMPLFRNIHHSPEYFADPHKFDPSRFEGKYGRFKFQVAPKPNTFMPFGSGVHTCPGN 428
           GWKVMPLFRNIHH+P+YF++P  FDPSRFE         V PKPNTFMPFGSGVH CPGN
Sbjct: 371 GWKVMPLFRNIHHNPKYFSNPEVFDPSRFE---------VNPKPNTFMPFGSGVHACPGN 430

Query: 429 ELAKLEILILIHHLVTKFRWELVGSQNGVQHSPFPVPSQGLPARF 474
           ELAKL+ILI +HHLV+ FRWE+ G + G+Q+SPFP+P  GLPA F
Sbjct: 431 ELAKLQILIFLHHLVSNFRWEVKGGEKGIQYSPFPIPQNGLPATF 463

BLAST of CmoCh01G018990 vs. TAIR 10
Match: AT5G45340.1 (cytochrome P450, family 707, subfamily A, polypeptide 3 )

HSP 1 Score: 552.0 bits (1421), Expect = 4.9e-157
Identity = 262/468 (55.98%), Postives = 351/468 (75.00%), Query Frame = 0

Query: 7   SLLLFFLIVLSYIYLNRNKRAGIRDGAK----LPPGSLGWPYIGETLQLYSQDPDLFFAA 66
           S    FL +L +I       AG+R  +     LPPG++G+PY+GET QLYSQDP++FFAA
Sbjct: 11  SAAALFLCLLRFI-------AGVRRSSSTKLPLPPGTMGYPYVGETFQLYSQDPNVFFAA 70

Query: 67  KQKRYGEIFKTHILGCPCVMLASPEAARFVLVTQAHLFKPTYPKSKERLIGPSALFYHQG 126
           KQ+RYG +FKTH+LGCPCVM++SPEAA+FVLVT++HLFKPT+P SKER++G  A+F+HQG
Sbjct: 71  KQRRYGSVFKTHVLGCPCVMISSPEAAKFVLVTKSHLFKPTFPASKERMLGKQAIFFHQG 130

Query: 127 DYHCRLRKLVQSSLSLDVIRSLVPDIDSVTTSALHSWTGAHLMNTFHEMKKISFEVGILA 186
           DYH +LRKLV  +   D IR++VP I+S+   +L+SW G  L NT+ EMK  +F V +++
Sbjct: 131 DYHSKLRKLVLRAFMPDAIRNMVPHIESIAQESLNSWDGTQL-NTYQEMKTYTFNVALIS 190

Query: 187 IFGHLEAAYKEDLKKNYSILDAGYNSFPTNIPGTPYKKALSARKRLSKILGDIIHERREK 246
           I G  E  Y+EDLK+ Y IL+ GYNS P N+PGT + KA+ ARK L++IL +I+ +RR+ 
Sbjct: 191 ILGKDEVYYREDLKRCYYILEKGYNSMPINLPGTLFHKAMKARKELAQILANILSKRRQN 250

Query: 247 KVGKKDLLGCFLNSIDEEGEAISDAQMEDNIIGVLFAAQDTTASVMTWVVKYLYDQPKLL 306
                DLLG F+    E+   ++D Q+ DNIIGV+FAA+DTTASV+TW++KYL D P +L
Sbjct: 251 PSSHTDLLGSFM----EDKAGLTDEQIADNIIGVIFAARDTTASVLTWILKYLADNPTVL 310

Query: 307 QTLKKEQQEILEFNYEKNQPLNWVQTRNMPMSYKVVLESLRMASIISFTFREAVADVEYK 366
           + + +EQ  I + + ++ + L W  T+ MP++Y+V+ E+LR A+I+SFTFREAV DVEY+
Sbjct: 311 EAVTEEQMAIRK-DKKEGESLTWEDTKKMPLTYRVIQETLRAATILSFTFREAVEDVEYE 370

Query: 367 GYLIPKGWKVMPLFRNIHHSPEYFADPHKFDPSRFEGKYGRFKFQVAPKPNTFMPFGSGV 426
           GYLIPKGWKV+PLFRNIHH+ + F+DP KFDPSRFE         VAPKPNTFMPFGSG+
Sbjct: 371 GYLIPKGWKVLPLFRNIHHNADIFSDPGKFDPSRFE---------VAPKPNTFMPFGSGI 430

Query: 427 HTCPGNELAKLEILILIHHLVTKFRWELVGSQNGVQHSPFPVPSQGLP 471
           H+CPGNELAKLEI +LIHHL TK+RW +VG  +G+Q+ PF +P  GLP
Sbjct: 431 HSCPGNELAKLEISVLIHHLTTKYRWSIVGPSDGIQYGPFALPQNGLP 456

BLAST of CmoCh01G018990 vs. TAIR 10
Match: AT4G19230.1 (cytochrome P450, family 707, subfamily A, polypeptide 1 )

HSP 1 Score: 549.7 bits (1415), Expect = 2.4e-156
Identity = 253/436 (58.03%), Postives = 338/436 (77.52%), Query Frame = 0

Query: 35  LPPGSLGWPYIGETLQLYSQDPDLFFAAKQKRYGEIFKTHILGCPCVMLASPEAARFVLV 94
           LPPG++GWPY+GET QLYSQDP++FF +KQKRYG +FKTH+LGCPCVM++SPEAA+FVLV
Sbjct: 36  LPPGTMGWPYVGETFQLYSQDPNVFFQSKQKRYGSVFKTHVLGCPCVMISSPEAAKFVLV 95

Query: 95  TQAHLFKPTYPKSKERLIGPSALFYHQGDYHCRLRKLVQSSLSLDVIRSLVPDIDSVTTS 154
           T++HLFKPT+P SKER++G  A+F+HQGDYH +LRKLV  +   + IR++VPDI+S+   
Sbjct: 96  TKSHLFKPTFPASKERMLGKQAIFFHQGDYHAKLRKLVLRAFMPESIRNMVPDIESIAQD 155

Query: 155 ALHSWTGAHLMNTFHEMKKISFEVGILAIFGHLEAAYKEDLKKNYSILDAGYNSFPTNIP 214
           +L SW G  ++NT+ EMK  +F V +L+IFG  E  Y+EDLK+ Y IL+ GYNS P N+P
Sbjct: 156 SLRSWEGT-MINTYQEMKTYTFNVALLSIFGKDEVLYREDLKRCYYILEKGYNSMPVNLP 215

Query: 215 GTPYKKALSARKRLSKILGDIIHERREKKVGKKDLLGCFLNSIDEEGEAISDAQMEDNII 274
           GT + K++ ARK LS+IL  I+ ERR+      DLLG F+     + E ++D Q+ DNII
Sbjct: 216 GTLFHKSMKARKELSQILARILSERRQNGSSHNDLLGSFMG----DKEELTDEQIADNII 275

Query: 275 GVLFAAQDTTASVMTWVVKYLYDQPKLLQTLKKEQQEILEFNYEKNQPLNWVQTRNMPMS 334
           GV+FAA+DTTASVM+W++KYL + P +L+ + +EQ  I + + E+ + L W  T+ MP++
Sbjct: 276 GVIFAARDTTASVMSWILKYLAENPNVLEAVTEEQMAIRK-DKEEGESLTWGDTKKMPLT 335

Query: 335 YKVVLESLRMASIISFTFREAVADVEYKGYLIPKGWKVMPLFRNIHHSPEYFADPHKFDP 394
            +V+ E+LR+ASI+SFTFREAV DVEY+GYLIPKGWKV+PLFRNIHHS + F++P KFDP
Sbjct: 336 SRVIQETLRVASILSFTFREAVEDVEYEGYLIPKGWKVLPLFRNIHHSADIFSNPGKFDP 395

Query: 395 SRFEGKYGRFKFQVAPKPNTFMPFGSGVHTCPGNELAKLEILILIHHLVTKFRWELVGSQ 454
           SRFE         VAPKPNTFMPFG+G H+CPGNELAKLE+ I+IHHL TK+ W +VG+ 
Sbjct: 396 SRFE---------VAPKPNTFMPFGNGTHSCPGNELAKLEMSIMIHHLTTKYSWSIVGAS 455

Query: 455 NGVQHSPFPVPSQGLP 471
           +G+Q+ PF +P  GLP
Sbjct: 456 DGIQYGPFALPQNGLP 456

BLAST of CmoCh01G018990 vs. TAIR 10
Match: AT5G45340.2 (cytochrome P450, family 707, subfamily A, polypeptide 3 )

HSP 1 Score: 524.6 bits (1350), Expect = 8.3e-149
Identity = 258/460 (56.09%), Postives = 343/460 (74.57%), Query Frame = 0

Query: 7   SLLLFFLIVLSYIYLNRNKRAGIRDGAK----LPPGSLGWPYIGETLQLYSQDPDLFFAA 66
           S    FL +L +I       AG+R  +     LPPG++G+PY+GET QLYSQDP++FFAA
Sbjct: 11  SAAALFLCLLRFI-------AGVRRSSSTKLPLPPGTMGYPYVGETFQLYSQDPNVFFAA 70

Query: 67  KQKRYGEIFKTHILGCPCVMLASPEAARFVLVTQAHLFKPTYPKSKERLIGPSALFYHQG 126
           KQ+RYG +FKTH+LGCPCVM++SPEAA+FVLVT++HLFKPT+P SKER++G  A+F+HQG
Sbjct: 71  KQRRYGSVFKTHVLGCPCVMISSPEAAKFVLVTKSHLFKPTFPASKERMLGKQAIFFHQG 130

Query: 127 DYHCRLRKLVQSSLSLDVIRSLVPDIDSVTTSALHSWTGAHLMNTFHEMKKISFEVGILA 186
           DYH +LRKLV  +   D IR++VP I+S+   +L+SW G  L NT+ EMK  +F V +++
Sbjct: 131 DYHSKLRKLVLRAFMPDAIRNMVPHIESIAQESLNSWDGTQL-NTYQEMKTYTFNVALIS 190

Query: 187 IFGHLEAAYKEDLKKNYSILDAGYNSFPTNIPGTPYKKALSARKRLSKILGDIIHERREK 246
           I G  E  Y+EDLK+ Y IL+ GYNS P N+PGT + KA+ ARK L++IL +I+ +RR+ 
Sbjct: 191 ILGKDEVYYREDLKRCYYILEKGYNSMPINLPGTLFHKAMKARKELAQILANILSKRRQN 250

Query: 247 KVGKKDLLGCFLNSIDEEGEAISDAQMEDNIIGVLFAAQDTTASVMTWVVKYLYDQPKLL 306
                DLLG F+    E+   ++D Q+ DNIIGV+FAA+DTTASV+TW++KYL D P +L
Sbjct: 251 PSSHTDLLGSFM----EDKAGLTDEQIADNIIGVIFAARDTTASVLTWILKYLADNPTVL 310

Query: 307 QTLKKEQQEILEFNYEKNQPLNWVQTRNMPMSYKVVLESLRMASIISFTFREAVADVEYK 366
           + + +EQ  I + + ++ + L W  T+ MP++Y+V+ E+LR A+I+SFTFREAV DVEY+
Sbjct: 311 EAVTEEQMAIRK-DKKEGESLTWEDTKKMPLTYRVIQETLRAATILSFTFREAVEDVEYE 370

Query: 367 GYLIPKGWKVMPLFRNIHHSPEYFADPHKFDPSRFEGKYGRFKFQVAPKPNTFMPFGSGV 426
           GYLIPKGWKV+PLFRNIHH+ + F+DP KFDPSRFE         VAPKPNTFMPFGSG+
Sbjct: 371 GYLIPKGWKVLPLFRNIHHNADIFSDPGKFDPSRFE---------VAPKPNTFMPFGSGI 430

Query: 427 HTCPGNELAKLEILILIHHLVTKFRWELVGSQNGVQHSPF 463
           H+CPGNELAKLEI +LIHHL TK+R  LV  QN   +SPF
Sbjct: 431 HSCPGNELAKLEISVLIHHLTTKYR--LVHLQN--DNSPF 444

BLAST of CmoCh01G018990 vs. TAIR 10
Match: AT4G19230.2 (cytochrome P450, family 707, subfamily A, polypeptide 1 )

HSP 1 Score: 524.2 bits (1349), Expect = 1.1e-148
Identity = 249/432 (57.64%), Postives = 332/432 (76.85%), Query Frame = 0

Query: 35  LPPGSLGWPYIGETLQLYSQDPDLFFAAKQKRYGEIFKTHILGCPCVMLASPEAARFVLV 94
           LPPG++GWPY+GET QLYSQDP++FF +KQKRYG +FKTH+LGCPCVM++SPEAA+FVLV
Sbjct: 36  LPPGTMGWPYVGETFQLYSQDPNVFFQSKQKRYGSVFKTHVLGCPCVMISSPEAAKFVLV 95

Query: 95  TQAHLFKPTYPKSKERLIGPSALFYHQGDYHCRLRKLVQSSLSLDVIRSLVPDIDSVTTS 154
           T++HLFKPT+P SKER++G  A+F+HQGDYH +LRKLV  +   + IR++VPDI+S+   
Sbjct: 96  TKSHLFKPTFPASKERMLGKQAIFFHQGDYHAKLRKLVLRAFMPESIRNMVPDIESIAQD 155

Query: 155 ALHSWTGAHLMNTFHEMKKISFEVGILAIFGHLEAAYKEDLKKNYSILDAGYNSFPTNIP 214
           +L SW G  ++NT+ EMK  +F V +L+IFG  E  Y+EDLK+ Y IL+ GYNS P N+P
Sbjct: 156 SLRSWEGT-MINTYQEMKTYTFNVALLSIFGKDEVLYREDLKRCYYILEKGYNSMPVNLP 215

Query: 215 GTPYKKALSARKRLSKILGDIIHERREKKVGKKDLLGCFLNSIDEEGEAISDAQMEDNII 274
           GT + K++ ARK LS+IL  I+ ERR+      DLLG F+     + E ++D Q+ DNII
Sbjct: 216 GTLFHKSMKARKELSQILARILSERRQNGSSHNDLLGSFMG----DKEELTDEQIADNII 275

Query: 275 GVLFAAQDTTASVMTWVVKYLYDQPKLLQTLKKEQQEILEFNYEKNQPLNWVQTRNMPMS 334
           GV+FAA+DTTASVM+W++KYL + P +L+ + +EQ  I + + E+ + L W  T+ MP++
Sbjct: 276 GVIFAARDTTASVMSWILKYLAENPNVLEAVTEEQMAIRK-DKEEGESLTWGDTKKMPLT 335

Query: 335 YKVVLESLRMASIISFTFREAVADVEYKGYLIPKGWKVMPLFRNIHHSPEYFADPHKFDP 394
            +V+ E+LR+ASI+SFTFREAV DVEY+GYLIPKGWKV+PLFRNIHHS + F++P KFDP
Sbjct: 336 SRVIQETLRVASILSFTFREAVEDVEYEGYLIPKGWKVLPLFRNIHHSADIFSNPGKFDP 395

Query: 395 SRFEGKYGRFKFQVAPKPNTFMPFGSGVHTCPGNELAKLEILILIHHLVTKFRW-ELVGS 454
           SRFE         VAPKPNTFMPFG+G H+CPGNELAKLE+ I+IHHL TK+R+ +L+  
Sbjct: 396 SRFE---------VAPKPNTFMPFGNGTHSCPGNELAKLEMSIMIHHLTTKYRFGQLLER 452

Query: 455 QNG--VQHSPFP 464
             G  + HS FP
Sbjct: 456 ATGFSMGHSRFP 452

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
A9QNE71.8e-18867.51Abscisic acid 8'-hydroxylase CYP707A1 OS=Solanum lycopersicum OX=4081 GN=CYP707A... [more]
Q9LJK21.8e-18064.30Abscisic acid 8'-hydroxylase 4 OS=Arabidopsis thaliana OX=3702 GN=CYP707A4 PE=2 ... [more]
Q0J1853.7e-17863.52Abscisic acid 8'-hydroxylase 3 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP70... [more]
A2Z2122.4e-17763.31Abscisic acid 8'-hydroxylase 3 OS=Oryza sativa subsp. indica OX=39946 GN=CYP707A... [more]
Q09J781.8e-17260.57Abscisic acid 8'-hydroxylase 2 OS=Oryza sativa subsp. indica OX=39946 GN=CYP707A... [more]
Match NameE-valueIdentityDescription
A0A6J1FQ852.4e-28198.16abscisic acid 8'-hydroxylase 4-like OS=Cucurbita moschata OX=3662 GN=LOC11144597... [more]
A0A6J1IYR97.4e-27094.68abscisic acid 8'-hydroxylase 4-like OS=Cucurbita maxima OX=3661 GN=LOC111481123 ... [more]
A0A1S3BTH69.1e-23684.55abscisic acid 8'-hydroxylase 4-like OS=Cucumis melo OX=3656 GN=LOC103493539 PE=3... [more]
A0A0A0L3K11.2e-23283.30Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G639800 PE=3 SV=1[more]
A0A5A7V8C15.2e-23184.95Abscisic acid 8'-hydroxylase 4-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6... [more]
Match NameE-valueIdentityDescription
XP_022940325.15.0e-28198.16abscisic acid 8'-hydroxylase 4-like [Cucurbita moschata][more]
KAG7037803.19.5e-28097.55Abscisic acid 8'-hydroxylase 4, partial [Cucurbita argyrosperma subsp. argyrospe... [more]
XP_023525021.12.9e-27696.32abscisic acid 8'-hydroxylase 4-like [Cucurbita pepo subsp. pepo][more]
XP_022982231.11.5e-26994.68abscisic acid 8'-hydroxylase 4-like [Cucurbita maxima][more]
KAG6608475.11.3e-26896.80Abscisic acid 8'-hydroxylase, partial [Cucurbita argyrosperma subsp. sororia][more]
Match NameE-valueIdentityDescription
AT3G19270.11.3e-18164.30cytochrome P450, family 707, subfamily A, polypeptide 4 [more]
AT5G45340.14.9e-15755.98cytochrome P450, family 707, subfamily A, polypeptide 3 [more]
AT4G19230.12.4e-15658.03cytochrome P450, family 707, subfamily A, polypeptide 1 [more]
AT5G45340.28.3e-14956.09cytochrome P450, family 707, subfamily A, polypeptide 3 [more]
AT4G19230.21.1e-14857.64cytochrome P450, family 707, subfamily A, polypeptide 1 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002401Cytochrome P450, E-class, group IPRINTSPR00463EP450Icoord: 375..399
score: 28.45
coord: 268..285
score: 19.67
coord: 425..448
score: 28.39
coord: 64..83
score: 29.92
coord: 335..353
score: 22.99
coord: 288..314
score: 17.42
coord: 415..425
score: 46.32
IPR001128Cytochrome P450PRINTSPR00385P450coord: 279..296
score: 29.76
coord: 336..347
score: 33.62
coord: 416..425
score: 46.59
coord: 425..436
score: 39.37
IPR001128Cytochrome P450PFAMPF00067p450coord: 36..452
e-value: 1.2E-67
score: 228.7
IPR036396Cytochrome P450 superfamilyGENE3D1.10.630.10Cytochrome P450coord: 27..476
e-value: 1.2E-117
score: 395.4
IPR036396Cytochrome P450 superfamilySUPERFAMILY48264Cytochrome P450coord: 33..473
NoneNo IPR availablePIRSRPIRSR000047-1PIRSR000047-1coord: 10..455
e-value: 2.0E-34
score: 117.2
NoneNo IPR availablePANTHERPTHR24286CYTOCHROME P450 26coord: 8..474
NoneNo IPR availablePANTHERPTHR24286:SF103CYTOCHROME P450 FAMILY ABA 8'-HYDROXYLASEcoord: 8..474
IPR017972Cytochrome P450, conserved sitePROSITEPS00086CYTOCHROME_P450coord: 418..427

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh01G018990.1CmoCh01G018990.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016125 sterol metabolic process
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0010295 (+)-abscisic acid 8'-hydroxylase activity
molecular_function GO:0020037 heme binding
molecular_function GO:0005506 iron ion binding
molecular_function GO:0004497 monooxygenase activity
molecular_function GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen