CmoCh01G018680 (gene) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh01G018680
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
DescriptionIsoaspartyl peptidase/L-asparaginase
LocationCmo_Chr01: 13545180 .. 13546614 (-)
RNA-Seq ExpressionCmoCh01G018680
SyntenyCmoCh01G018680
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AGAGTGAAGAAGAAGAGAGAGAGCGATGGGAGGTTGGGCCATAGCGGTTCACGGCGGGGCTGGCGTGGATCCTAATCTCCCACTGCAACGTCAAGAAGATGCAAAAATGCTTCTCACTCGCTGCCTTGATCTTGGCATTCACGCTCTTCGTTCAAATTGTTCTGCCATTGATGTCGTTGAACTCGTCGTATGCTCTCTCCGATCCCTTTTCGGTTTCTTTTCTTTCTCTAAAACATCATTTCATTCTCTGCGAAATATTCTTAAATTATATTGCGATTCTGAAGGTTAGAGAACTTGAAACCGATCCCCTGTTCAACTCGGGGCGTGGATCTGCCCTGACAGAGGAGGGAACCGTTGAAATGGAAGCCAGCATCATGGACGGACCAAAACGGCGATGCGGGGCGGTGTCTGGACTTACCACTGTCAAAAACCCGATCTCCCTCGCCCGTCTTGTCATGGACAAATCCCCTCATTCCTATCTGGCTTTCTCTGGCGCTGAGAAATTCGCTAGAGAACAGGTTCGATTTCCATCTTTATTCTCCCTTTTTTCCGGTTCTAAAGCGGATTTTCTGACGGAATTGAATCTGTGTTAGGGTGTTGAACTAGAGTGCAATGACTATTTCGTCACCGAAGAAAACGTCGGAATGCTTGAACTAGCCAGAGAAGCCAACAGTATATGGGTACCCCTCCGATTCAAATATTTTAACTGAATATCATTCAAAATAACCGTTCGAAACCGACTTAATATTTTGTGTTTCGATTTGTAACAGTTAGATTATAGGGTCCCAAACGAGAAGTGTAGCACGAGCGTGGAGACGCCGTTGCAAATGAACGGCCTCCCAATCAGCGTGTACGCGCCAGAGACTGTGGGCTGCGTGGTGGTCGACAGTCAAGGAAGGTGCGCCGCCGCCACTTCAACCGGCGGCCTGATGAACAAAAAGATCGGGCGCATCGGAGACTCTCCGCTGATCGGTTCAGGGACGTACGCGTGCGGGGTGTGCGGGGTATCGTGCACTGGAGAAGGGGAGGCGATCATAAGAGGGACATTGGCTAGAGAAGTAGCGGCGGTGATGGAATACAAAGGTCTAGGGCTTCAAGAGGCAGTGAATTACGTGATAGAGGAGAGGCTGGACGAAGGAAAGGCCGGATTGATTGCCGTGTCGAGCAATGGTGAAGTGGTATGTGGGTTCAACACCACCGGAATGTTCAGGGGATGTGCGACGGAGGATGGGTTTATGGAGGTTGGGATTTGGAAATAAATTGGGATATGATTGGTTAGATAATTTAAGAATGGGATGAAGTTTTAACGCTCTGTAGTGTGCTTTGTTTTTTCAGTAACTGTGTGAATCTTCTTCAGAAATTTCTGGATTCAGATTCTGAGGCAGATCCATCTTAGCAAATAAAAATAATGCCCTTAGCGCTGACTACTACGACC

mRNA sequence

AGAGTGAAGAAGAAGAGAGAGAGCGATGGGAGGTTGGGCCATAGCGGTTCACGGCGGGGCTGGCGTGGATCCTAATCTCCCACTGCAACGTCAAGAAGATGCAAAAATGCTTCTCACTCGCTGCCTTGATCTTGGCATTCACGCTCTTCGTTCAAATTGTTCTGCCATTGATGTCGTTGAACTCGTCGTTAGAGAACTTGAAACCGATCCCCTGTTCAACTCGGGGCGTGGATCTGCCCTGACAGAGGAGGGAACCGTTGAAATGGAAGCCAGCATCATGGACGGACCAAAACGGCGATGCGGGGCGGTGTCTGGACTTACCACTGTCAAAAACCCGATCTCCCTCGCCCGTCTTGTCATGGACAAATCCCCTCATTCCTATCTGGCTTTCTCTGGCGCTGAGAAATTCGCTAGAGAACAGGGTGTTGAACTAGAGTGCAATGACTATTTCGTCACCGAAGAAAACGTCGGAATGCTTGAACTAGCCAGAGAAGCCAACAGTATATGGTTAGATTATAGGGTCCCAAACGAGAAGTGTAGCACGAGCGTGGAGACGCCGTTGCAAATGAACGGCCTCCCAATCAGCGTGTACGCGCCAGAGACTGTGGGCTGCGTGGTGGTCGACAGTCAAGGAAGGTGCGCCGCCGCCACTTCAACCGGCGGCCTGATGAACAAAAAGATCGGGCGCATCGGAGACTCTCCGCTGATCGGTTCAGGGACGTACGCGTGCGGGGTGTGCGGGGTATCGTGCACTGGAGAAGGGGAGGCGATCATAAGAGGGACATTGGCTAGAGAAGTAGCGGCGGTGATGGAATACAAAGGTCTAGGGCTTCAAGAGGCAGTGAATTACGTGATAGAGGAGAGGCTGGACGAAGGAAAGGCCGGATTGATTGCCGTGTCGAGCAATGGTGAAGTGGTATGTGGGTTCAACACCACCGGAATGTTCAGGGGATGTGCGACGGAGGATGGGTTTATGGAGGTTGGGATTTGGAAATAAATTGGGATATGATTGGTTAGATAATTTAAGAATGGGATGAAGTTTTAACGCTCTGTAGTGTGCTTTGTTTTTTCAGTAACTGTGTGAATCTTCTTCAGAAATTTCTGGATTCAGATTCTGAGGCAGATCCATCTTAGCAAATAAAAATAATGCCCTTAGCGCTGACTACTACGACC

Coding sequence (CDS)

ATGGGAGGTTGGGCCATAGCGGTTCACGGCGGGGCTGGCGTGGATCCTAATCTCCCACTGCAACGTCAAGAAGATGCAAAAATGCTTCTCACTCGCTGCCTTGATCTTGGCATTCACGCTCTTCGTTCAAATTGTTCTGCCATTGATGTCGTTGAACTCGTCGTTAGAGAACTTGAAACCGATCCCCTGTTCAACTCGGGGCGTGGATCTGCCCTGACAGAGGAGGGAACCGTTGAAATGGAAGCCAGCATCATGGACGGACCAAAACGGCGATGCGGGGCGGTGTCTGGACTTACCACTGTCAAAAACCCGATCTCCCTCGCCCGTCTTGTCATGGACAAATCCCCTCATTCCTATCTGGCTTTCTCTGGCGCTGAGAAATTCGCTAGAGAACAGGGTGTTGAACTAGAGTGCAATGACTATTTCGTCACCGAAGAAAACGTCGGAATGCTTGAACTAGCCAGAGAAGCCAACAGTATATGGTTAGATTATAGGGTCCCAAACGAGAAGTGTAGCACGAGCGTGGAGACGCCGTTGCAAATGAACGGCCTCCCAATCAGCGTGTACGCGCCAGAGACTGTGGGCTGCGTGGTGGTCGACAGTCAAGGAAGGTGCGCCGCCGCCACTTCAACCGGCGGCCTGATGAACAAAAAGATCGGGCGCATCGGAGACTCTCCGCTGATCGGTTCAGGGACGTACGCGTGCGGGGTGTGCGGGGTATCGTGCACTGGAGAAGGGGAGGCGATCATAAGAGGGACATTGGCTAGAGAAGTAGCGGCGGTGATGGAATACAAAGGTCTAGGGCTTCAAGAGGCAGTGAATTACGTGATAGAGGAGAGGCTGGACGAAGGAAAGGCCGGATTGATTGCCGTGTCGAGCAATGGTGAAGTGGTATGTGGGTTCAACACCACCGGAATGTTCAGGGGATGTGCGACGGAGGATGGGTTTATGGAGGTTGGGATTTGGAAATAA

Protein sequence

MGGWAIAVHGGAGVDPNLPLQRQEDAKMLLTRCLDLGIHALRSNCSAIDVVELVVRELETDPLFNSGRGSALTEEGTVEMEASIMDGPKRRCGAVSGLTTVKNPISLARLVMDKSPHSYLAFSGAEKFAREQGVELECNDYFVTEENVGMLELAREANSIWLDYRVPNEKCSTSVETPLQMNGLPISVYAPETVGCVVVDSQGRCAAATSTGGLMNKKIGRIGDSPLIGSGTYACGVCGVSCTGEGEAIIRGTLAREVAAVMEYKGLGLQEAVNYVIEERLDEGKAGLIAVSSNGEVVCGFNTTGMFRGCATEDGFMEVGIWK
Homology
BLAST of CmoCh01G018680 vs. ExPASy Swiss-Prot
Match: Q8GXG1 (Probable isoaspartyl peptidase/L-asparaginase 2 OS=Arabidopsis thaliana OX=3702 GN=At3g16150 PE=2 SV=2)

HSP 1 Score: 535.0 bits (1377), Expect = 5.7e-151
Identity = 263/325 (80.92%), Postives = 294/325 (90.46%), Query Frame = 0

Query: 1   MGGWAIAVHGGAGVDPNLPLQRQEDAKMLLTRCLDLGIHALRSNCSAIDVVELVVRELET 60
           MGGWAIAVHGGAG+DPNLP +RQE+AK LLTRCL+LGI ALRSN SAIDVVELV+RELET
Sbjct: 1   MGGWAIAVHGGAGIDPNLPAERQEEAKQLLTRCLNLGIIALRSNVSAIDVVELVIRELET 60

Query: 61  DPLFNSGRGSALTEEGTVEMEASIMDGPKRRCGAVSGLTTVKNPISLARLVMDKSPHSYL 120
           DPLFNSGRGSALTE+GTVEMEASIMDG KRRCGAVSG+TTVKNPISLARLVMDKSPHSYL
Sbjct: 61  DPLFNSGRGSALTEKGTVEMEASIMDGTKRRCGAVSGITTVKNPISLARLVMDKSPHSYL 120

Query: 121 AFSGAEKFAREQGVELECNDYFVTEENVGMLELAREANSIWLDYRVPNEKC--STSVETP 180
           AFSGAE FAR+QGVE+  N+YFVT++NVGML+LA+EANSI  DYR+P   C  + + ++P
Sbjct: 121 AFSGAEDFARKQGVEIVDNEYFVTDDNVGMLKLAKEANSILFDYRIPPMGCAGAAATDSP 180

Query: 181 LQMNGLPISVYAPETVGCVVVDSQGRCAAATSTGGLMNKKIGRIGDSPLIGSGTYACGVC 240
           +QMNGLPIS+YAPETVGCVVVD +G CAA TSTGGLMNK +GRIGDSPLIG+GTYA   C
Sbjct: 181 IQMNGLPISIYAPETVGCVVVDGKGHCAAGTSTGGLMNKMMGRIGDSPLIGAGTYASEFC 240

Query: 241 GVSCTGEGEAIIRGTLAREVAAVMEYKGLGLQEAVNYVIEERLDEGKAGLIAVSSNGEVV 300
           GVSCTGEGEAIIR TLAR+V+AVMEYKGL LQEAV+YVI+ RLDEG AGLIAVS+ GEVV
Sbjct: 241 GVSCTGEGEAIIRATLARDVSAVMEYKGLNLQEAVDYVIKHRLDEGFAGLIAVSNKGEVV 300

Query: 301 CGFNTTGMFRGCATEDGFMEVGIWK 324
           CGFN+ GMFRGCATEDGFMEV IW+
Sbjct: 301 CGFNSNGMFRGCATEDGFMEVAIWE 325

BLAST of CmoCh01G018680 vs. ExPASy Swiss-Prot
Match: P50287 (Isoaspartyl peptidase/L-asparaginase 1 OS=Arabidopsis thaliana OX=3702 GN=At5g08100 PE=1 SV=2)

HSP 1 Score: 365.2 bits (936), Expect = 7.9e-100
Identity = 190/323 (58.82%), Postives = 232/323 (71.83%), Query Frame = 0

Query: 1   MGGWAIAVHGGAGVDP-NLPLQRQEDAKMLLTRCLDLGIHALRSNCSAIDVVELVVRELE 60
           M GWAIA+HGGAG  P +LP +R+   +  L  CLDLGI AL+S    +DV ELVVRELE
Sbjct: 1   MVGWAIALHGGAGDIPIDLPDERRIPRESALRHCLDLGISALKSGKPPLDVAELVVRELE 60

Query: 61  TDPLFNSGRGSALTEEGTVEMEASIMDGPKRRCGAVSGLTTVKNPISLARLVMDKSPHSY 120
             P FN+G+GS LT +GTVEMEASIMDG  +RCGAVSGLTTV NPISLARLVM+K+PH Y
Sbjct: 61  NHPDFNAGKGSVLTAQGTVEMEASIMDGKTKRCGAVSGLTTVVNPISLARLVMEKTPHIY 120

Query: 121 LAFSGAEKFAREQGVELECNDYFVTEENVGMLELAREANSIWLDYRVPNEKCSTSVETPL 180
           LAF  AE FAR  GVE   + +F+T EN+  L+ A+E N + LDY VP+ K       P 
Sbjct: 121 LAFDAAEAFARAHGVETVDSSHFITPENIARLKQAKEFNRVQLDYTVPSPK------VPD 180

Query: 181 QMNGLPISVYAPETVGCVVVDSQGRCAAATSTGGLMNKKIGRIGDSPLIGSGTYACGVCG 240
                 I      TVGCV VDS G  A+ATSTGG +NK +GRIGD+P+IG+GTYA  +C 
Sbjct: 181 NCGDSQIG-----TVGCVAVDSAGNLASATSTGGYVNKMVGRIGDTPVIGAGTYANHLCA 240

Query: 241 VSCTGEGEAIIRGTLAREVAAVMEYKGLGLQEAVNYVIEERLDEGKAGLIAVSSNGEVVC 300
           +S TG+GE IIRGT+AR+VAA+MEYKGL L EA  YV+++ +  G  GL+AVS+NGEV  
Sbjct: 241 ISATGKGEDIIRGTVARDVAALMEYKGLSLTEAAAYVVDQSVPRGSCGLVAVSANGEVTM 300

Query: 301 GFNTTGMFRGCATEDGFMEVGIW 323
            FNTTGMFR CA+EDG+ E+ IW
Sbjct: 301 PFNTTGMFRACASEDGYSEIAIW 312

BLAST of CmoCh01G018680 vs. ExPASy Swiss-Prot
Match: P30364 (Isoaspartyl peptidase/L-asparaginase OS=Lupinus angustifolius OX=3871 PE=2 SV=1)

HSP 1 Score: 358.2 bits (918), Expect = 9.6e-98
Identity = 190/327 (58.10%), Postives = 234/327 (71.56%), Query Frame = 0

Query: 1   MGGWAIAVHGGAGVDP-NLPLQRQEDAKMLLTRCLDLGIHALRSNCSAIDVVELVVRELE 60
           MGGW+IA+HGGAG  P +LP +R++  +  L  CL +G+ AL+S    +DVVELVVRELE
Sbjct: 1   MGGWSIALHGGAGDIPFSLPPERRQPREEGLRHCLQIGVEALKSQKPPLDVVELVVRELE 60

Query: 61  TDPLFNSGRGSALTEEGTVEMEASIMDGPKRRCGAVSGLTTVKNPISLARLVMDKSPHSY 120
               FN+G GS LT  GTVEMEASIMDG   +CGAVSGL+TV NPISLARLVMDK+PH Y
Sbjct: 61  NIQHFNAGIGSVLTNSGTVEMEASIMDGKTMKCGAVSGLSTVLNPISLARLVMDKTPHIY 120

Query: 121 LAFSGAEKFAREQGVELECNDYFVTEENVGMLELAREANSIWLDYRVPN----EKCSTSV 180
           LAF GA+ FA++QGVE   + +F+T ENV  L+LA EAN + +DY   N     +     
Sbjct: 121 LAFQGAQDFAKQQGVETVDSSHFITAENVERLKLAIEANRVQVDYSQYNYPQPAQDDAEK 180

Query: 181 ETPLQMNGLPISVYAPETVGCVVVDSQGRCAAATSTGGLMNKKIGRIGDSPLIGSGTYAC 240
           E PL      I      TVGCV VDS G  A+ATSTGGL+NK +GRIGD+PLIG+GTYA 
Sbjct: 181 ELPLANGDSQIG-----TVGCVAVDSHGNLASATSTGGLVNKMVGRIGDTPLIGAGTYAN 240

Query: 241 GVCGVSCTGEGEAIIRGTLAREVAAVMEYKGLGLQEAVNYVIEERLDEGKAGLIAVSSNG 300
            +C VS TG+GEAII  T+AR+VAA+ME+KGL L+EA +YV+ ER  +G  GLIAVS+ G
Sbjct: 241 ELCAVSATGKGEAIISATVARDVAALMEFKGLSLKEAADYVVHERTPKGTVGLIAVSAAG 300

Query: 301 EVVCGFNTTGMFRGCATEDGFMEVGIW 323
           E+   FNTTGMFR  ATEDG+ E+ IW
Sbjct: 301 EIAMPFNTTGMFRASATEDGYSEIAIW 322

BLAST of CmoCh01G018680 vs. ExPASy Swiss-Prot
Match: Q9ZSD6 (Isoaspartyl peptidase/L-asparaginase OS=Lupinus luteus OX=3873 PE=1 SV=1)

HSP 1 Score: 358.2 bits (918), Expect = 9.6e-98
Identity = 190/327 (58.10%), Postives = 234/327 (71.56%), Query Frame = 0

Query: 1   MGGWAIAVHGGAGVDP-NLPLQRQEDAKMLLTRCLDLGIHALRSNCSAIDVVELVVRELE 60
           MGGW+IA+HGGAG  P +LP +R++  +  L  CL +G+ AL++    +DVVELVVRELE
Sbjct: 1   MGGWSIALHGGAGDIPFSLPPERRKPREEGLRHCLQIGVEALKAQKPPLDVVELVVRELE 60

Query: 61  TDPLFNSGRGSALTEEGTVEMEASIMDGPKRRCGAVSGLTTVKNPISLARLVMDKSPHSY 120
               FN+G GS LT  GTVEMEASIMDG   +CGAVSGL+TV NPISLARLVMDK+PH Y
Sbjct: 61  NIEHFNAGIGSVLTNSGTVEMEASIMDGNTMKCGAVSGLSTVLNPISLARLVMDKTPHIY 120

Query: 121 LAFSGAEKFAREQGVELECNDYFVTEENVGMLELAREANSIWLDYRVPN----EKCSTSV 180
           LAF GA+ FA++QGVE   + + +T ENV  L+LA EAN + +DY   N     K     
Sbjct: 121 LAFQGAQDFAKQQGVETVDSSHLITAENVERLKLAIEANRVQVDYSQYNYPEPVKDDAEK 180

Query: 181 ETPLQMNGLPISVYAPETVGCVVVDSQGRCAAATSTGGLMNKKIGRIGDSPLIGSGTYAC 240
           E PL      I      TVGCV VDS G  A+ATSTGGL+NK +GRIGD+PLIG+GTYA 
Sbjct: 181 ELPLTNGDSQIG-----TVGCVAVDSHGNLASATSTGGLVNKMVGRIGDTPLIGAGTYAN 240

Query: 241 GVCGVSCTGEGEAIIRGTLAREVAAVMEYKGLGLQEAVNYVIEERLDEGKAGLIAVSSNG 300
            +C VS TG+GE IIR T+AR+VAA+ME+KGL L+EA ++VI ER  +G  GLIAVS+ G
Sbjct: 241 ELCAVSATGKGEEIIRATVARDVAALMEFKGLSLKEAADFVIHERTPKGTVGLIAVSAAG 300

Query: 301 EVVCGFNTTGMFRGCATEDGFMEVGIW 323
           E+   FNTTGMFR CATEDG+ E+ IW
Sbjct: 301 EIAMPFNTTGMFRACATEDGYSEIAIW 322

BLAST of CmoCh01G018680 vs. ExPASy Swiss-Prot
Match: P50288 (Isoaspartyl peptidase/L-asparaginase OS=Lupinus albus OX=3870 PE=3 SV=1)

HSP 1 Score: 353.6 bits (906), Expect = 2.4e-96
Identity = 188/327 (57.49%), Postives = 232/327 (70.95%), Query Frame = 0

Query: 1   MGGWAIAVHGGAGVDP-NLPLQRQEDAKMLLTRCLDLGIHALRSNCSAIDVVELVVRELE 60
           MGGW+IA+HGGAG  P +LP +R++  +  L  CL +GI AL++    +DVVELVVRELE
Sbjct: 1   MGGWSIALHGGAGDIPFSLPPERRKPREEGLRHCLQIGIEALKAQNPPLDVVELVVRELE 60

Query: 61  TDPLFNSGRGSALTEEGTVEMEASIMDGPKRRCGAVSGLTTVKNPISLARLVMDKSPHSY 120
               FN+G GS LT  GTVEMEASIMDG   +CGAVSGL TV NPISLAR VMDK+PH +
Sbjct: 61  NIQHFNAGIGSVLTNSGTVEMEASIMDGNTMKCGAVSGLNTVMNPISLARQVMDKTPHIF 120

Query: 121 LAFSGAEKFAREQGVELECNDYFVTEENVGMLELAREANSIWLDYRVPN----EKCSTSV 180
           LAF GA+   ++QGVE   + +F+TEENV  L+LA EAN + +DY   N     +     
Sbjct: 121 LAFQGAQDLGKQQGVETVDSSHFITEENVERLKLAIEANRVQVDYSQYNYTQPVQDDAEK 180

Query: 181 ETPLQMNGLPISVYAPETVGCVVVDSQGRCAAATSTGGLMNKKIGRIGDSPLIGSGTYAC 240
           E PL      I      TVGCV VDS G  A+ATSTGGL+NK +GRIGD+PLIG+GTYA 
Sbjct: 181 ELPLANGDSQIG-----TVGCVAVDSHGNLASATSTGGLVNKMVGRIGDTPLIGAGTYAN 240

Query: 241 GVCGVSCTGEGEAIIRGTLAREVAAVMEYKGLGLQEAVNYVIEERLDEGKAGLIAVSSNG 300
            +C VS TG+GEAIIR T+AR+VAA+ME+KGL L+EA + V+ ER  +G  GLIAVS+ G
Sbjct: 241 ELCAVSATGKGEAIIRATVARDVAALMEFKGLSLKEAADCVVHERTPKGTVGLIAVSAAG 300

Query: 301 EVVCGFNTTGMFRGCATEDGFMEVGIW 323
           E+   FNTTGMFR CATEDG+ E+ IW
Sbjct: 301 EIAMPFNTTGMFRACATEDGYSEIAIW 322

BLAST of CmoCh01G018680 vs. ExPASy TrEMBL
Match: A0A6J1FND2 (probable isoaspartyl peptidase/L-asparaginase 2 OS=Cucurbita moschata OX=3662 GN=LOC111446743 PE=4 SV=1)

HSP 1 Score: 652.5 bits (1682), Expect = 9.1e-184
Identity = 323/323 (100.00%), Postives = 323/323 (100.00%), Query Frame = 0

Query: 1   MGGWAIAVHGGAGVDPNLPLQRQEDAKMLLTRCLDLGIHALRSNCSAIDVVELVVRELET 60
           MGGWAIAVHGGAGVDPNLPLQRQEDAKMLLTRCLDLGIHALRSNCSAIDVVELVVRELET
Sbjct: 1   MGGWAIAVHGGAGVDPNLPLQRQEDAKMLLTRCLDLGIHALRSNCSAIDVVELVVRELET 60

Query: 61  DPLFNSGRGSALTEEGTVEMEASIMDGPKRRCGAVSGLTTVKNPISLARLVMDKSPHSYL 120
           DPLFNSGRGSALTEEGTVEMEASIMDGPKRRCGAVSGLTTVKNPISLARLVMDKSPHSYL
Sbjct: 61  DPLFNSGRGSALTEEGTVEMEASIMDGPKRRCGAVSGLTTVKNPISLARLVMDKSPHSYL 120

Query: 121 AFSGAEKFAREQGVELECNDYFVTEENVGMLELAREANSIWLDYRVPNEKCSTSVETPLQ 180
           AFSGAEKFAREQGVELECNDYFVTEENVGMLELAREANSIWLDYRVPNEKCSTSVETPLQ
Sbjct: 121 AFSGAEKFAREQGVELECNDYFVTEENVGMLELAREANSIWLDYRVPNEKCSTSVETPLQ 180

Query: 181 MNGLPISVYAPETVGCVVVDSQGRCAAATSTGGLMNKKIGRIGDSPLIGSGTYACGVCGV 240
           MNGLPISVYAPETVGCVVVDSQGRCAAATSTGGLMNKKIGRIGDSPLIGSGTYACGVCGV
Sbjct: 181 MNGLPISVYAPETVGCVVVDSQGRCAAATSTGGLMNKKIGRIGDSPLIGSGTYACGVCGV 240

Query: 241 SCTGEGEAIIRGTLAREVAAVMEYKGLGLQEAVNYVIEERLDEGKAGLIAVSSNGEVVCG 300
           SCTGEGEAIIRGTLAREVAAVMEYKGLGLQEAVNYVIEERLDEGKAGLIAVSSNGEVVCG
Sbjct: 241 SCTGEGEAIIRGTLAREVAAVMEYKGLGLQEAVNYVIEERLDEGKAGLIAVSSNGEVVCG 300

Query: 301 FNTTGMFRGCATEDGFMEVGIWK 324
           FNTTGMFRGCATEDGFMEVGIWK
Sbjct: 301 FNTTGMFRGCATEDGFMEVGIWK 323

BLAST of CmoCh01G018680 vs. ExPASy TrEMBL
Match: A0A6J1IYV7 (probable isoaspartyl peptidase/L-asparaginase 2 OS=Cucurbita maxima OX=3661 GN=LOC111481149 PE=4 SV=1)

HSP 1 Score: 649.4 bits (1674), Expect = 7.7e-183
Identity = 321/323 (99.38%), Postives = 322/323 (99.69%), Query Frame = 0

Query: 1   MGGWAIAVHGGAGVDPNLPLQRQEDAKMLLTRCLDLGIHALRSNCSAIDVVELVVRELET 60
           MGGWAIAVHGGAGVDPNLPLQRQEDAKMLLTRCLDLGIHALRSNCSAIDVVELVVRELET
Sbjct: 1   MGGWAIAVHGGAGVDPNLPLQRQEDAKMLLTRCLDLGIHALRSNCSAIDVVELVVRELET 60

Query: 61  DPLFNSGRGSALTEEGTVEMEASIMDGPKRRCGAVSGLTTVKNPISLARLVMDKSPHSYL 120
           DPLFNSGRGSALTEEGTVEMEASIMDGPKRRCGAVSGLTTVKNPISLARLVMDKSPHSYL
Sbjct: 61  DPLFNSGRGSALTEEGTVEMEASIMDGPKRRCGAVSGLTTVKNPISLARLVMDKSPHSYL 120

Query: 121 AFSGAEKFAREQGVELECNDYFVTEENVGMLELAREANSIWLDYRVPNEKCSTSVETPLQ 180
           AFSGAEKFAREQGVELECNDYFVTEENVGMLELAREANSIWLDYRVPNEKCS SVETPLQ
Sbjct: 121 AFSGAEKFAREQGVELECNDYFVTEENVGMLELAREANSIWLDYRVPNEKCSASVETPLQ 180

Query: 181 MNGLPISVYAPETVGCVVVDSQGRCAAATSTGGLMNKKIGRIGDSPLIGSGTYACGVCGV 240
           MNGLPISVYAPETVGCVVVDS+GRCAAATSTGGLMNKKIGRIGDSPLIGSGTYACGVCGV
Sbjct: 181 MNGLPISVYAPETVGCVVVDSEGRCAAATSTGGLMNKKIGRIGDSPLIGSGTYACGVCGV 240

Query: 241 SCTGEGEAIIRGTLAREVAAVMEYKGLGLQEAVNYVIEERLDEGKAGLIAVSSNGEVVCG 300
           SCTGEGEAIIRGTLAREVAAVMEYKGLGLQEAVNYVIEERLDEGKAGLIAVSSNGEVVCG
Sbjct: 241 SCTGEGEAIIRGTLAREVAAVMEYKGLGLQEAVNYVIEERLDEGKAGLIAVSSNGEVVCG 300

Query: 301 FNTTGMFRGCATEDGFMEVGIWK 324
           FNTTGMFRGCATEDGFMEVGIWK
Sbjct: 301 FNTTGMFRGCATEDGFMEVGIWK 323

BLAST of CmoCh01G018680 vs. ExPASy TrEMBL
Match: A0A6J1CHT1 (probable isoaspartyl peptidase/L-asparaginase 2 OS=Momordica charantia OX=3673 GN=LOC111011731 PE=4 SV=1)

HSP 1 Score: 587.0 bits (1512), Expect = 4.7e-164
Identity = 290/325 (89.23%), Postives = 308/325 (94.77%), Query Frame = 0

Query: 1   MGGWAIAVHGGAGVDPNLPLQRQEDAKMLLTRCLDLGIHALRSNCSAIDVVELVVRELET 60
           M GWAIAVHGGAGVDPNLPL RQE+AK LL RCL+LGI ALRS+  AIDVVELVVRELE+
Sbjct: 1   MAGWAIAVHGGAGVDPNLPLDRQEEAKKLLARCLNLGIDALRSDHPAIDVVELVVRELES 60

Query: 61  DPLFNSGRGSALTEEGTVEMEASIMDGPKRRCGAVSGLTTVKNPISLARLVMDKSPHSYL 120
           DPLFNSGRGSALTE+GTVEMEASIMDGPKRRCGAVSGLTTVKNPISLARLVMDKSPHSYL
Sbjct: 61  DPLFNSGRGSALTEDGTVEMEASIMDGPKRRCGAVSGLTTVKNPISLARLVMDKSPHSYL 120

Query: 121 AFSGAEKFAREQGVELECNDYFVTEENVGMLELAREANSIWLDYRVPN---EKCSTSVET 180
           AFSGAE+FAR+QGVE+ CNDYF+TEENVGML+LA+EANSI  DYR+PN   EKCS SVET
Sbjct: 121 AFSGAEEFARQQGVEMVCNDYFITEENVGMLKLAKEANSILFDYRIPNAGLEKCSASVET 180

Query: 181 PLQMNGLPISVYAPETVGCVVVDSQGRCAAATSTGGLMNKKIGRIGDSPLIGSGTYACGV 240
           PLQMNGLPISVYAPETVGCVVVDSQGRCAAATSTGGLMNK+ GRIGDSPLIG+GTYACG+
Sbjct: 181 PLQMNGLPISVYAPETVGCVVVDSQGRCAAATSTGGLMNKRSGRIGDSPLIGAGTYACGL 240

Query: 241 CGVSCTGEGEAIIRGTLAREVAAVMEYKGLGLQEAVNYVIEERLDEGKAGLIAVSSNGEV 300
           CGVSCTGEGEAIIRGTLAREVAAVMEYKG+GLQEAV+YV+EERLDEGKAGLIAVSSNGEV
Sbjct: 241 CGVSCTGEGEAIIRGTLAREVAAVMEYKGMGLQEAVSYVVEERLDEGKAGLIAVSSNGEV 300

Query: 301 VCGFNTTGMFRGCATEDGFMEVGIW 323
            CGFNTTGMFRGCATE GFMEVGIW
Sbjct: 301 ACGFNTTGMFRGCATEAGFMEVGIW 325

BLAST of CmoCh01G018680 vs. ExPASy TrEMBL
Match: A0A5D3D9K2 (Putative isoaspartyl peptidase/L-asparaginase 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold134G003020 PE=4 SV=1)

HSP 1 Score: 579.7 bits (1493), Expect = 7.5e-162
Identity = 288/322 (89.44%), Postives = 299/322 (92.86%), Query Frame = 0

Query: 1   MGGWAIAVHGGAGVDPNLPLQRQEDAKMLLTRCLDLGIHALRSNCSAIDVVELVVRELET 60
           MGGWAIAVHGGAGVDPNLPL RQ+DAK  LTRCLDLGIHALRSN SAIDVVELVVRELE 
Sbjct: 1   MGGWAIAVHGGAGVDPNLPLHRQDDAKRFLTRCLDLGIHALRSNHSAIDVVELVVRELEN 60

Query: 61  DPLFNSGRGSALTEEGTVEMEASIMDGPKRRCGAVSGLTTVKNPISLARLVMDKSPHSYL 120
           DPLFNSGRGSALTE+GTVEMEASIMDGPKRRCGAVSGL TVKNPISLARLVMDKSPHSYL
Sbjct: 61  DPLFNSGRGSALTEDGTVEMEASIMDGPKRRCGAVSGLVTVKNPISLARLVMDKSPHSYL 120

Query: 121 AFSGAEKFAREQGVELECNDYFVTEENVGMLELAREANSIWLDYRVPNEKCSTSVETPLQ 180
           AFSGAEKFAR+QGVEL  NDYF+TE NV +L+LA+EAN IWLD RVP E C  SVETPLQ
Sbjct: 121 AFSGAEKFARQQGVELVSNDYFITEGNVELLQLAKEANGIWLDRRVPVETCCASVETPLQ 180

Query: 181 MNGLPISVYAPETVGCVVVDSQGRCAAATSTGGLMNKKIGRIGDSPLIGSGTYACGVCGV 240
           MN +PISVYAPETVGCVVVDS+GRCAAATSTGGL+NKK+GRIGDSPLIG GTYAC VCGV
Sbjct: 181 MNSVPISVYAPETVGCVVVDSEGRCAAATSTGGLINKKVGRIGDSPLIGLGTYACDVCGV 240

Query: 241 SCTGEGEAIIRGTLAREVAAVMEYKGLGLQEAVNYVIEERLDEGKAGLIAVSSNGEVVCG 300
           SCTGEGEAIIRGTLAREVAAVMEYKGLGLQEAVNYVIEERLDEG AGLIAVSSNGEV CG
Sbjct: 241 SCTGEGEAIIRGTLAREVAAVMEYKGLGLQEAVNYVIEERLDEGLAGLIAVSSNGEVACG 300

Query: 301 FNTTGMFRGCATEDGFMEVGIW 323
           FNTTGMFRG ATEDGFMEV IW
Sbjct: 301 FNTTGMFRGFATEDGFMEVAIW 322

BLAST of CmoCh01G018680 vs. ExPASy TrEMBL
Match: A0A1S3BV13 (probable isoaspartyl peptidase/L-asparaginase 2 OS=Cucumis melo OX=3656 GN=LOC103493576 PE=4 SV=1)

HSP 1 Score: 579.7 bits (1493), Expect = 7.5e-162
Identity = 288/322 (89.44%), Postives = 299/322 (92.86%), Query Frame = 0

Query: 1   MGGWAIAVHGGAGVDPNLPLQRQEDAKMLLTRCLDLGIHALRSNCSAIDVVELVVRELET 60
           MGGWAIAVHGGAGVDPNLPL RQ+DAK  LTRCLDLGIHALRSN SAIDVVELVVRELE 
Sbjct: 1   MGGWAIAVHGGAGVDPNLPLHRQDDAKRFLTRCLDLGIHALRSNHSAIDVVELVVRELEN 60

Query: 61  DPLFNSGRGSALTEEGTVEMEASIMDGPKRRCGAVSGLTTVKNPISLARLVMDKSPHSYL 120
           DPLFNSGRGSALTE+GTVEMEASIMDGPKRRCGAVSGL TVKNPISLARLVMDKSPHSYL
Sbjct: 61  DPLFNSGRGSALTEDGTVEMEASIMDGPKRRCGAVSGLVTVKNPISLARLVMDKSPHSYL 120

Query: 121 AFSGAEKFAREQGVELECNDYFVTEENVGMLELAREANSIWLDYRVPNEKCSTSVETPLQ 180
           AFSGAEKFAR+QGVEL  NDYF+TE NV +L+LA+EAN IWLD RVP E C  SVETPLQ
Sbjct: 121 AFSGAEKFARQQGVELVSNDYFITEGNVELLQLAKEANGIWLDRRVPVETCCASVETPLQ 180

Query: 181 MNGLPISVYAPETVGCVVVDSQGRCAAATSTGGLMNKKIGRIGDSPLIGSGTYACGVCGV 240
           MN +PISVYAPETVGCVVVDS+GRCAAATSTGGL+NKK+GRIGDSPLIG GTYAC VCGV
Sbjct: 181 MNSVPISVYAPETVGCVVVDSEGRCAAATSTGGLINKKVGRIGDSPLIGLGTYACDVCGV 240

Query: 241 SCTGEGEAIIRGTLAREVAAVMEYKGLGLQEAVNYVIEERLDEGKAGLIAVSSNGEVVCG 300
           SCTGEGEAIIRGTLAREVAAVMEYKGLGLQEAVNYVIEERLDEG AGLIAVSSNGEV CG
Sbjct: 241 SCTGEGEAIIRGTLAREVAAVMEYKGLGLQEAVNYVIEERLDEGLAGLIAVSSNGEVACG 300

Query: 301 FNTTGMFRGCATEDGFMEVGIW 323
           FNTTGMFRG ATEDGFMEV IW
Sbjct: 301 FNTTGMFRGFATEDGFMEVAIW 322

BLAST of CmoCh01G018680 vs. NCBI nr
Match: XP_022941449.1 (probable isoaspartyl peptidase/L-asparaginase 2 [Cucurbita moschata] >KAG6608447.1 putative isoaspartyl peptidase/L-asparaginase 2, partial [Cucurbita argyrosperma subsp. sororia] >KAG7037783.1 putative isoaspartyl peptidase/L-asparaginase 2, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 652.5 bits (1682), Expect = 1.9e-183
Identity = 323/323 (100.00%), Postives = 323/323 (100.00%), Query Frame = 0

Query: 1   MGGWAIAVHGGAGVDPNLPLQRQEDAKMLLTRCLDLGIHALRSNCSAIDVVELVVRELET 60
           MGGWAIAVHGGAGVDPNLPLQRQEDAKMLLTRCLDLGIHALRSNCSAIDVVELVVRELET
Sbjct: 1   MGGWAIAVHGGAGVDPNLPLQRQEDAKMLLTRCLDLGIHALRSNCSAIDVVELVVRELET 60

Query: 61  DPLFNSGRGSALTEEGTVEMEASIMDGPKRRCGAVSGLTTVKNPISLARLVMDKSPHSYL 120
           DPLFNSGRGSALTEEGTVEMEASIMDGPKRRCGAVSGLTTVKNPISLARLVMDKSPHSYL
Sbjct: 61  DPLFNSGRGSALTEEGTVEMEASIMDGPKRRCGAVSGLTTVKNPISLARLVMDKSPHSYL 120

Query: 121 AFSGAEKFAREQGVELECNDYFVTEENVGMLELAREANSIWLDYRVPNEKCSTSVETPLQ 180
           AFSGAEKFAREQGVELECNDYFVTEENVGMLELAREANSIWLDYRVPNEKCSTSVETPLQ
Sbjct: 121 AFSGAEKFAREQGVELECNDYFVTEENVGMLELAREANSIWLDYRVPNEKCSTSVETPLQ 180

Query: 181 MNGLPISVYAPETVGCVVVDSQGRCAAATSTGGLMNKKIGRIGDSPLIGSGTYACGVCGV 240
           MNGLPISVYAPETVGCVVVDSQGRCAAATSTGGLMNKKIGRIGDSPLIGSGTYACGVCGV
Sbjct: 181 MNGLPISVYAPETVGCVVVDSQGRCAAATSTGGLMNKKIGRIGDSPLIGSGTYACGVCGV 240

Query: 241 SCTGEGEAIIRGTLAREVAAVMEYKGLGLQEAVNYVIEERLDEGKAGLIAVSSNGEVVCG 300
           SCTGEGEAIIRGTLAREVAAVMEYKGLGLQEAVNYVIEERLDEGKAGLIAVSSNGEVVCG
Sbjct: 241 SCTGEGEAIIRGTLAREVAAVMEYKGLGLQEAVNYVIEERLDEGKAGLIAVSSNGEVVCG 300

Query: 301 FNTTGMFRGCATEDGFMEVGIWK 324
           FNTTGMFRGCATEDGFMEVGIWK
Sbjct: 301 FNTTGMFRGCATEDGFMEVGIWK 323

BLAST of CmoCh01G018680 vs. NCBI nr
Match: XP_022982271.1 (probable isoaspartyl peptidase/L-asparaginase 2 [Cucurbita maxima])

HSP 1 Score: 649.4 bits (1674), Expect = 1.6e-182
Identity = 321/323 (99.38%), Postives = 322/323 (99.69%), Query Frame = 0

Query: 1   MGGWAIAVHGGAGVDPNLPLQRQEDAKMLLTRCLDLGIHALRSNCSAIDVVELVVRELET 60
           MGGWAIAVHGGAGVDPNLPLQRQEDAKMLLTRCLDLGIHALRSNCSAIDVVELVVRELET
Sbjct: 1   MGGWAIAVHGGAGVDPNLPLQRQEDAKMLLTRCLDLGIHALRSNCSAIDVVELVVRELET 60

Query: 61  DPLFNSGRGSALTEEGTVEMEASIMDGPKRRCGAVSGLTTVKNPISLARLVMDKSPHSYL 120
           DPLFNSGRGSALTEEGTVEMEASIMDGPKRRCGAVSGLTTVKNPISLARLVMDKSPHSYL
Sbjct: 61  DPLFNSGRGSALTEEGTVEMEASIMDGPKRRCGAVSGLTTVKNPISLARLVMDKSPHSYL 120

Query: 121 AFSGAEKFAREQGVELECNDYFVTEENVGMLELAREANSIWLDYRVPNEKCSTSVETPLQ 180
           AFSGAEKFAREQGVELECNDYFVTEENVGMLELAREANSIWLDYRVPNEKCS SVETPLQ
Sbjct: 121 AFSGAEKFAREQGVELECNDYFVTEENVGMLELAREANSIWLDYRVPNEKCSASVETPLQ 180

Query: 181 MNGLPISVYAPETVGCVVVDSQGRCAAATSTGGLMNKKIGRIGDSPLIGSGTYACGVCGV 240
           MNGLPISVYAPETVGCVVVDS+GRCAAATSTGGLMNKKIGRIGDSPLIGSGTYACGVCGV
Sbjct: 181 MNGLPISVYAPETVGCVVVDSEGRCAAATSTGGLMNKKIGRIGDSPLIGSGTYACGVCGV 240

Query: 241 SCTGEGEAIIRGTLAREVAAVMEYKGLGLQEAVNYVIEERLDEGKAGLIAVSSNGEVVCG 300
           SCTGEGEAIIRGTLAREVAAVMEYKGLGLQEAVNYVIEERLDEGKAGLIAVSSNGEVVCG
Sbjct: 241 SCTGEGEAIIRGTLAREVAAVMEYKGLGLQEAVNYVIEERLDEGKAGLIAVSSNGEVVCG 300

Query: 301 FNTTGMFRGCATEDGFMEVGIWK 324
           FNTTGMFRGCATEDGFMEVGIWK
Sbjct: 301 FNTTGMFRGCATEDGFMEVGIWK 323

BLAST of CmoCh01G018680 vs. NCBI nr
Match: XP_023525179.1 (probable isoaspartyl peptidase/L-asparaginase 2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 642.1 bits (1655), Expect = 2.5e-180
Identity = 321/324 (99.07%), Postives = 321/324 (99.07%), Query Frame = 0

Query: 1   MGGWAIAVHGGAGVDPNLPLQRQEDAKMLLTRCLDLGIHALRSNCSAIDVVELVVRELET 60
           MGGWAIAVHGGAGVDPNLPLQRQEDAKMLLTRCLDLGIHALRSN SAIDVVELVVRELET
Sbjct: 1   MGGWAIAVHGGAGVDPNLPLQRQEDAKMLLTRCLDLGIHALRSNSSAIDVVELVVRELET 60

Query: 61  DPLFNSGRGSALTEEGTVEMEASIMDGPKRRCGAVSGLTTVKNPISLARLVMDKSPHSYL 120
           DPLFNSGRGSALTEEGTVEMEASIMDGPKRRCGAVSGLTTVKNPISLARLVMDKSPHSYL
Sbjct: 61  DPLFNSGRGSALTEEGTVEMEASIMDGPKRRCGAVSGLTTVKNPISLARLVMDKSPHSYL 120

Query: 121 AFSGAEKFAREQGVELECNDYFVTEENVGMLELAREANSIWLDYRVP-NEKCSTSVETPL 180
           AFSGAEKFAREQGVELECNDYFVTEENVGMLELAREANSIWLDYRVP NEKCS SVETPL
Sbjct: 121 AFSGAEKFAREQGVELECNDYFVTEENVGMLELAREANSIWLDYRVPTNEKCSASVETPL 180

Query: 181 QMNGLPISVYAPETVGCVVVDSQGRCAAATSTGGLMNKKIGRIGDSPLIGSGTYACGVCG 240
           QMNGLPISVYAPETVGCVVVDSQGRCAAATSTGGLMNKKIGRIGDSPLIGSGTYACGVCG
Sbjct: 181 QMNGLPISVYAPETVGCVVVDSQGRCAAATSTGGLMNKKIGRIGDSPLIGSGTYACGVCG 240

Query: 241 VSCTGEGEAIIRGTLAREVAAVMEYKGLGLQEAVNYVIEERLDEGKAGLIAVSSNGEVVC 300
           VSCTGEGEAIIRGTLAREVAAVMEYKGLGLQEAVNYVIEERLDEGKAGLIAVSSNGEVVC
Sbjct: 241 VSCTGEGEAIIRGTLAREVAAVMEYKGLGLQEAVNYVIEERLDEGKAGLIAVSSNGEVVC 300

Query: 301 GFNTTGMFRGCATEDGFMEVGIWK 324
           GFNTTGMFRGCATEDGFMEVGIWK
Sbjct: 301 GFNTTGMFRGCATEDGFMEVGIWK 324

BLAST of CmoCh01G018680 vs. NCBI nr
Match: XP_038896168.1 (probable isoaspartyl peptidase/L-asparaginase 2 [Benincasa hispida])

HSP 1 Score: 604.0 bits (1556), Expect = 7.7e-169
Identity = 298/322 (92.55%), Postives = 310/322 (96.27%), Query Frame = 0

Query: 1   MGGWAIAVHGGAGVDPNLPLQRQEDAKMLLTRCLDLGIHALRSNCSAIDVVELVVRELET 60
           MGGWAIAVHGGAGVDPNLPL RQ DAK LLTRCLDLGIHALRSN SAIDVVELVVRELE 
Sbjct: 1   MGGWAIAVHGGAGVDPNLPLHRQHDAKKLLTRCLDLGIHALRSNHSAIDVVELVVRELEN 60

Query: 61  DPLFNSGRGSALTEEGTVEMEASIMDGPKRRCGAVSGLTTVKNPISLARLVMDKSPHSYL 120
           DPLFNSGRGSALTEEGTVEMEASIMDGPKRRCGAVSG+TTVKNPISLARLVMDKSPHSYL
Sbjct: 61  DPLFNSGRGSALTEEGTVEMEASIMDGPKRRCGAVSGITTVKNPISLARLVMDKSPHSYL 120

Query: 121 AFSGAEKFAREQGVELECNDYFVTEENVGMLELAREANSIWLDYRVPNEKCSTSVETPLQ 180
           AFSGAEKFAR+QGVEL  NDYF+TE+NVG+LELA+EANSIWLD+RVP+E CS SVETPLQ
Sbjct: 121 AFSGAEKFARQQGVELVGNDYFITEDNVGLLELAKEANSIWLDHRVPSETCSASVETPLQ 180

Query: 181 MNGLPISVYAPETVGCVVVDSQGRCAAATSTGGLMNKKIGRIGDSPLIGSGTYACGVCGV 240
           MNGL ISVYAPETVGCVVVD++GRCAAATSTGGL+NKK+GRIGDSPLIGSGTYAC VCGV
Sbjct: 181 MNGLRISVYAPETVGCVVVDTEGRCAAATSTGGLINKKMGRIGDSPLIGSGTYACDVCGV 240

Query: 241 SCTGEGEAIIRGTLAREVAAVMEYKGLGLQEAVNYVIEERLDEGKAGLIAVSSNGEVVCG 300
           SCTGEGEAIIRGTLAREVAAVMEYKGLGLQEAVNYVIEERLDEGKAGLIAVSSNGEV CG
Sbjct: 241 SCTGEGEAIIRGTLAREVAAVMEYKGLGLQEAVNYVIEERLDEGKAGLIAVSSNGEVACG 300

Query: 301 FNTTGMFRGCATEDGFMEVGIW 323
           FNTTGMFRGCATEDGFMEVGIW
Sbjct: 301 FNTTGMFRGCATEDGFMEVGIW 322

BLAST of CmoCh01G018680 vs. NCBI nr
Match: XP_022141340.1 (probable isoaspartyl peptidase/L-asparaginase 2 [Momordica charantia])

HSP 1 Score: 587.0 bits (1512), Expect = 9.7e-164
Identity = 290/325 (89.23%), Postives = 308/325 (94.77%), Query Frame = 0

Query: 1   MGGWAIAVHGGAGVDPNLPLQRQEDAKMLLTRCLDLGIHALRSNCSAIDVVELVVRELET 60
           M GWAIAVHGGAGVDPNLPL RQE+AK LL RCL+LGI ALRS+  AIDVVELVVRELE+
Sbjct: 1   MAGWAIAVHGGAGVDPNLPLDRQEEAKKLLARCLNLGIDALRSDHPAIDVVELVVRELES 60

Query: 61  DPLFNSGRGSALTEEGTVEMEASIMDGPKRRCGAVSGLTTVKNPISLARLVMDKSPHSYL 120
           DPLFNSGRGSALTE+GTVEMEASIMDGPKRRCGAVSGLTTVKNPISLARLVMDKSPHSYL
Sbjct: 61  DPLFNSGRGSALTEDGTVEMEASIMDGPKRRCGAVSGLTTVKNPISLARLVMDKSPHSYL 120

Query: 121 AFSGAEKFAREQGVELECNDYFVTEENVGMLELAREANSIWLDYRVPN---EKCSTSVET 180
           AFSGAE+FAR+QGVE+ CNDYF+TEENVGML+LA+EANSI  DYR+PN   EKCS SVET
Sbjct: 121 AFSGAEEFARQQGVEMVCNDYFITEENVGMLKLAKEANSILFDYRIPNAGLEKCSASVET 180

Query: 181 PLQMNGLPISVYAPETVGCVVVDSQGRCAAATSTGGLMNKKIGRIGDSPLIGSGTYACGV 240
           PLQMNGLPISVYAPETVGCVVVDSQGRCAAATSTGGLMNK+ GRIGDSPLIG+GTYACG+
Sbjct: 181 PLQMNGLPISVYAPETVGCVVVDSQGRCAAATSTGGLMNKRSGRIGDSPLIGAGTYACGL 240

Query: 241 CGVSCTGEGEAIIRGTLAREVAAVMEYKGLGLQEAVNYVIEERLDEGKAGLIAVSSNGEV 300
           CGVSCTGEGEAIIRGTLAREVAAVMEYKG+GLQEAV+YV+EERLDEGKAGLIAVSSNGEV
Sbjct: 241 CGVSCTGEGEAIIRGTLAREVAAVMEYKGMGLQEAVSYVVEERLDEGKAGLIAVSSNGEV 300

Query: 301 VCGFNTTGMFRGCATEDGFMEVGIW 323
            CGFNTTGMFRGCATE GFMEVGIW
Sbjct: 301 ACGFNTTGMFRGCATEAGFMEVGIW 325

BLAST of CmoCh01G018680 vs. TAIR 10
Match: AT3G16150.1 (N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein )

HSP 1 Score: 535.0 bits (1377), Expect = 4.1e-152
Identity = 263/325 (80.92%), Postives = 294/325 (90.46%), Query Frame = 0

Query: 1   MGGWAIAVHGGAGVDPNLPLQRQEDAKMLLTRCLDLGIHALRSNCSAIDVVELVVRELET 60
           MGGWAIAVHGGAG+DPNLP +RQE+AK LLTRCL+LGI ALRSN SAIDVVELV+RELET
Sbjct: 1   MGGWAIAVHGGAGIDPNLPAERQEEAKQLLTRCLNLGIIALRSNVSAIDVVELVIRELET 60

Query: 61  DPLFNSGRGSALTEEGTVEMEASIMDGPKRRCGAVSGLTTVKNPISLARLVMDKSPHSYL 120
           DPLFNSGRGSALTE+GTVEMEASIMDG KRRCGAVSG+TTVKNPISLARLVMDKSPHSYL
Sbjct: 61  DPLFNSGRGSALTEKGTVEMEASIMDGTKRRCGAVSGITTVKNPISLARLVMDKSPHSYL 120

Query: 121 AFSGAEKFAREQGVELECNDYFVTEENVGMLELAREANSIWLDYRVPNEKC--STSVETP 180
           AFSGAE FAR+QGVE+  N+YFVT++NVGML+LA+EANSI  DYR+P   C  + + ++P
Sbjct: 121 AFSGAEDFARKQGVEIVDNEYFVTDDNVGMLKLAKEANSILFDYRIPPMGCAGAAATDSP 180

Query: 181 LQMNGLPISVYAPETVGCVVVDSQGRCAAATSTGGLMNKKIGRIGDSPLIGSGTYACGVC 240
           +QMNGLPIS+YAPETVGCVVVD +G CAA TSTGGLMNK +GRIGDSPLIG+GTYA   C
Sbjct: 181 IQMNGLPISIYAPETVGCVVVDGKGHCAAGTSTGGLMNKMMGRIGDSPLIGAGTYASEFC 240

Query: 241 GVSCTGEGEAIIRGTLAREVAAVMEYKGLGLQEAVNYVIEERLDEGKAGLIAVSSNGEVV 300
           GVSCTGEGEAIIR TLAR+V+AVMEYKGL LQEAV+YVI+ RLDEG AGLIAVS+ GEVV
Sbjct: 241 GVSCTGEGEAIIRATLARDVSAVMEYKGLNLQEAVDYVIKHRLDEGFAGLIAVSNKGEVV 300

Query: 301 CGFNTTGMFRGCATEDGFMEVGIWK 324
           CGFN+ GMFRGCATEDGFMEV IW+
Sbjct: 301 CGFNSNGMFRGCATEDGFMEVAIWE 325

BLAST of CmoCh01G018680 vs. TAIR 10
Match: AT5G08100.1 (N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein )

HSP 1 Score: 365.2 bits (936), Expect = 5.6e-101
Identity = 190/323 (58.82%), Postives = 232/323 (71.83%), Query Frame = 0

Query: 1   MGGWAIAVHGGAGVDP-NLPLQRQEDAKMLLTRCLDLGIHALRSNCSAIDVVELVVRELE 60
           M GWAIA+HGGAG  P +LP +R+   +  L  CLDLGI AL+S    +DV ELVVRELE
Sbjct: 1   MVGWAIALHGGAGDIPIDLPDERRIPRESALRHCLDLGISALKSGKPPLDVAELVVRELE 60

Query: 61  TDPLFNSGRGSALTEEGTVEMEASIMDGPKRRCGAVSGLTTVKNPISLARLVMDKSPHSY 120
             P FN+G+GS LT +GTVEMEASIMDG  +RCGAVSGLTTV NPISLARLVM+K+PH Y
Sbjct: 61  NHPDFNAGKGSVLTAQGTVEMEASIMDGKTKRCGAVSGLTTVVNPISLARLVMEKTPHIY 120

Query: 121 LAFSGAEKFAREQGVELECNDYFVTEENVGMLELAREANSIWLDYRVPNEKCSTSVETPL 180
           LAF  AE FAR  GVE   + +F+T EN+  L+ A+E N + LDY VP+ K       P 
Sbjct: 121 LAFDAAEAFARAHGVETVDSSHFITPENIARLKQAKEFNRVQLDYTVPSPK------VPD 180

Query: 181 QMNGLPISVYAPETVGCVVVDSQGRCAAATSTGGLMNKKIGRIGDSPLIGSGTYACGVCG 240
                 I      TVGCV VDS G  A+ATSTGG +NK +GRIGD+P+IG+GTYA  +C 
Sbjct: 181 NCGDSQIG-----TVGCVAVDSAGNLASATSTGGYVNKMVGRIGDTPVIGAGTYANHLCA 240

Query: 241 VSCTGEGEAIIRGTLAREVAAVMEYKGLGLQEAVNYVIEERLDEGKAGLIAVSSNGEVVC 300
           +S TG+GE IIRGT+AR+VAA+MEYKGL L EA  YV+++ +  G  GL+AVS+NGEV  
Sbjct: 241 ISATGKGEDIIRGTVARDVAALMEYKGLSLTEAAAYVVDQSVPRGSCGLVAVSANGEVTM 300

Query: 301 GFNTTGMFRGCATEDGFMEVGIW 323
            FNTTGMFR CA+EDG+ E+ IW
Sbjct: 301 PFNTTGMFRACASEDGYSEIAIW 312

BLAST of CmoCh01G018680 vs. TAIR 10
Match: AT5G08100.2 (N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein )

HSP 1 Score: 280.0 bits (715), Expect = 2.4e-75
Identity = 143/243 (58.85%), Postives = 175/243 (72.02%), Query Frame = 0

Query: 80  MEASIMDGPKRRCGAVSGLTTVKNPISLARLVMDKSPHSYLAFSGAEKFAREQGVELECN 139
           MEASIMDG  +RCGAVSGLTTV NPISLARLVM+K+PH YLAF  AE FAR  GVE   +
Sbjct: 1   MEASIMDGKTKRCGAVSGLTTVVNPISLARLVMEKTPHIYLAFDAAEAFARAHGVETVDS 60

Query: 140 DYFVTEENVGMLELAREANSIWLDYRVPNEKCSTSVETPLQMNGLPISVYAPETVGCVVV 199
            +F+T EN+  L+ A+E N + LDY VP+ K       P       I      TVGCV V
Sbjct: 61  SHFITPENIARLKQAKEFNRVQLDYTVPSPK------VPDNCGDSQIG-----TVGCVAV 120

Query: 200 DSQGRCAAATSTGGLMNKKIGRIGDSPLIGSGTYACGVCGVSCTGEGEAIIRGTLAREVA 259
           DS G  A+ATSTGG +NK +GRIGD+P+IG+GTYA  +C +S TG+GE IIRGT+AR+VA
Sbjct: 121 DSAGNLASATSTGGYVNKMVGRIGDTPVIGAGTYANHLCAISATGKGEDIIRGTVARDVA 180

Query: 260 AVMEYKGLGLQEAVNYVIEERLDEGKAGLIAVSSNGEVVCGFNTTGMFRGCATEDGFMEV 319
           A+MEYKGL L EA  YV+++ +  G  GL+AVS+NGEV   FNTTGMFR CA+EDG+ E+
Sbjct: 181 ALMEYKGLSLTEAAAYVVDQSVPRGSCGLVAVSANGEVTMPFNTTGMFRACASEDGYSEI 232

Query: 320 GIW 323
            IW
Sbjct: 241 AIW 232

BLAST of CmoCh01G018680 vs. TAIR 10
Match: AT4G00590.1 (N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein )

HSP 1 Score: 103.2 bits (256), Expect = 4.0e-22
Identity = 91/336 (27.08%), Postives = 142/336 (42.26%), Query Frame = 0

Query: 6   IAVHGGAGVDPNLPLQRQEDAKMLLTRCLDLGIHALRSNCSAIDVVELVVRELETDPLFN 65
           +AVH GAG   +     +    ++   CL       + +   ID V   ++ LE DP  N
Sbjct: 15  VAVHVGAGY--HAAANEKALRSVMRRACLAASTILRQDSGECIDAVSAAIQVLEDDPSTN 74

Query: 66  SGRGSALTEEGTVEMEASIMDGPKRRCGAVSGLTTVKNPISLARL----------VMDKS 125
           +GRGS LTE+G VE +AS+MDG     G V  +  V+N I +A L          ++ + 
Sbjct: 75  AGRGSNLTEDGHVECDASLMDGDSGMFGGVGAVPGVRNAIKIAALLVKEQITGSTLLGRI 134

Query: 126 PHSYLAFSGAEKFAREQ-----GVELECNDYFVTEENVGMLELARE------ANSIWLDY 185
           P   L   GA ++ + +     G   E + + VTE         +       A SI    
Sbjct: 135 PPMLLVGEGARRWGKSKSVLIPGTVTEADQWLVTERARNQWRRFKAMLSEVGAKSILSAE 194

Query: 186 RVP-----NEKCSTSVETPLQMNGLPISVYAPETVGCVVVDSQGRCAAATSTGGLMNKKI 245
             P     NE C  +V +    +   I     +TVG + VD++G  A  +S+GG+  K  
Sbjct: 195 EHPRGTENNETCEENVSSCAAADEDKIM----DTVGVICVDNEGHIACGSSSGGIAMKIS 254

Query: 246 GRIGDSPLIGSGTYACG--------VCGVSCTGEGEAIIRGTLAREVAAVMEYKGLGLQE 299
           GR+G +   GSG +A          + G   +G GE ++RG  ARE    +     G   
Sbjct: 255 GRVGLAATYGSGCWASSKGPFGAPFLVGCCVSGAGEYLMRGFAARECCTSLALSQAGPAS 314

BLAST of CmoCh01G018680 vs. TAIR 10
Match: AT4G00590.2 (N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein )

HSP 1 Score: 92.8 bits (229), Expect = 5.4e-19
Identity = 89/328 (27.13%), Postives = 141/328 (42.99%), Query Frame = 0

Query: 6   IAVHGGAGVDPNLPLQRQEDAKMLLTRCLDLGIHALRSNCSAIDVVELVVRELETDPLFN 65
           +AVH GAG   +     +    ++   CL       + +   ID V   ++ LE DP  N
Sbjct: 15  VAVHVGAGY--HAAANEKALRSVMRRACLAASTILRQDSGECIDAVSAAIQVLEDDPSTN 74

Query: 66  SGRGSALTEEGTVEMEASIMDGPKRRCGAVSGLTTVKNPIS--LARLVMDKSPHSYLAFS 125
           +GRGS LTE+G VE +AS+MDG     G   G+  V + +S  L++L++       L   
Sbjct: 75  AGRGSNLTEDGHVECDASLMDGDS---GMFGGVGAVPDQVSEMLSKLLL----CWLLVGE 134

Query: 126 GAEKFAREQ-----GVELECNDYFVTEENVGMLELARE------ANSIWLDYRVP----- 185
           GA ++ + +     G   E + + VTE         +       A SI      P     
Sbjct: 135 GARRWGKSKSVLIPGTVTEADQWLVTERARNQWRRFKAMLSEVGAKSILSAEEHPRGTEN 194

Query: 186 NEKCSTSVETPLQMNGLPISVYAPETVGCVVVDSQGRCAAATSTGGLMNKKIGRIGDSPL 245
           NE C  +V +    +   I     +TVG + VD++G  A  +S+GG+  K  GR+G +  
Sbjct: 195 NETCEENVSSCAAADEDKIM----DTVGVICVDNEGHIACGSSSGGIAMKISGRVGLAAT 254

Query: 246 IGSGTYACG--------VCGVSCTGEGEAIIRGTLAREVAAVMEYKGLGLQEAVNYVIEE 299
            GSG +A          + G   +G GE ++RG  ARE    +     G   A   V+  
Sbjct: 255 YGSGCWASSKGPFGAPFLVGCCVSGAGEYLMRGFAARECCTSLALSQAGPASAAMKVLRS 314

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8GXG15.7e-15180.92Probable isoaspartyl peptidase/L-asparaginase 2 OS=Arabidopsis thaliana OX=3702 ... [more]
P502877.9e-10058.82Isoaspartyl peptidase/L-asparaginase 1 OS=Arabidopsis thaliana OX=3702 GN=At5g08... [more]
P303649.6e-9858.10Isoaspartyl peptidase/L-asparaginase OS=Lupinus angustifolius OX=3871 PE=2 SV=1[more]
Q9ZSD69.6e-9858.10Isoaspartyl peptidase/L-asparaginase OS=Lupinus luteus OX=3873 PE=1 SV=1[more]
P502882.4e-9657.49Isoaspartyl peptidase/L-asparaginase OS=Lupinus albus OX=3870 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1FND29.1e-184100.00probable isoaspartyl peptidase/L-asparaginase 2 OS=Cucurbita moschata OX=3662 GN... [more]
A0A6J1IYV77.7e-18399.38probable isoaspartyl peptidase/L-asparaginase 2 OS=Cucurbita maxima OX=3661 GN=L... [more]
A0A6J1CHT14.7e-16489.23probable isoaspartyl peptidase/L-asparaginase 2 OS=Momordica charantia OX=3673 G... [more]
A0A5D3D9K27.5e-16289.44Putative isoaspartyl peptidase/L-asparaginase 2 OS=Cucumis melo var. makuwa OX=1... [more]
A0A1S3BV137.5e-16289.44probable isoaspartyl peptidase/L-asparaginase 2 OS=Cucumis melo OX=3656 GN=LOC10... [more]
Match NameE-valueIdentityDescription
XP_022941449.11.9e-183100.00probable isoaspartyl peptidase/L-asparaginase 2 [Cucurbita moschata] >KAG6608447... [more]
XP_022982271.11.6e-18299.38probable isoaspartyl peptidase/L-asparaginase 2 [Cucurbita maxima][more]
XP_023525179.12.5e-18099.07probable isoaspartyl peptidase/L-asparaginase 2 [Cucurbita pepo subsp. pepo][more]
XP_038896168.17.7e-16992.55probable isoaspartyl peptidase/L-asparaginase 2 [Benincasa hispida][more]
XP_022141340.19.7e-16489.23probable isoaspartyl peptidase/L-asparaginase 2 [Momordica charantia][more]
Match NameE-valueIdentityDescription
AT3G16150.14.1e-15280.92N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein [more]
AT5G08100.15.6e-10158.82N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein [more]
AT5G08100.22.4e-7558.85N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein [more]
AT4G00590.14.0e-2227.08N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein [more]
AT4G00590.25.4e-1927.13N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.60.20.30(Glycosyl)asparaginasecoord: 193..323
e-value: 2.9E-43
score: 149.1
NoneNo IPR availablePIRSRPIRSR600246-2PIRSR600246-2coord: 3..319
e-value: 2.2E-90
score: 300.9
NoneNo IPR availablePIRSRPIRSR600246-1PIRSR600246-1coord: 3..319
e-value: 2.2E-90
score: 300.9
NoneNo IPR availablePIRSRPIRSR600246-3PIRSR600246-3coord: 3..319
e-value: 2.2E-90
score: 300.9
NoneNo IPR availablePANTHERPTHR10188:SF36PEPTIDASE T2, ASPARAGINASE 2, NUCLEOPHILE AMINOHYDROLASE-RELATEDcoord: 1..322
NoneNo IPR availableCDDcd04701Asparaginase_2coord: 5..315
e-value: 6.62138E-123
score: 351.378
IPR000246Peptidase T2, asparaginase 2PFAMPF01112Asparaginase_2coord: 4..322
e-value: 7.7E-101
score: 337.2
IPR000246Peptidase T2, asparaginase 2PANTHERPTHR10188L-ASPARAGINASEcoord: 1..322
IPR029055Nucleophile aminohydrolases, N-terminalSUPERFAMILY56235N-terminal nucleophile aminohydrolases (Ntn hydrolases)coord: 4..320

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh01G018680.1CmoCh01G018680.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016540 protein autoprocessing
cellular_component GO:0005737 cytoplasm
molecular_function GO:0004067 asparaginase activity
molecular_function GO:0008798 beta-aspartyl-peptidase activity
molecular_function GO:0016787 hydrolase activity