CmoCh01G015400 (gene) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh01G015400
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
DescriptionDNA ligase
LocationCmo_Chr01: 11889843 .. 11890217 (-)
RNA-Seq ExpressionCmoCh01G015400
SyntenyCmoCh01G015400
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGATGTTGAACGACTACCATCCATTTTCATTTACTCGCAAGCTCAAATCCTCAATCCTCTGTTTCCGTCGCGATCATGGCGATTTCCTCGAAAACGAAGGCGATTCTTCTCCAAGAACCCCTAGATCGCCGCTGAAATCAATGGCCGCAGAGTTCCCGGAGCTTCGAGGAATGTGCAGGAATCTCGTCGCTCGGATCGGCAGGTCTCGCCGCCGGTACCATCACTCCGCCGATTTCCGATACGATCCTTCTAGCTATGCGCTTAATTTCGAGGACGAACAGGTTCGGGATGATGATGAGTTTCCGATTAGGGATTTCGCTTCCAGATTGCCGGCGTCTCCGCCGACTTCCCCGAGACTTTCGCCCGCGAAATGA

mRNA sequence

ATGATGTTGAACGACTACCATCCATTTTCATTTACTCGCAAGCTCAAATCCTCAATCCTCTGTTTCCGTCGCGATCATGGCGATTTCCTCGAAAACGAAGGCGATTCTTCTCCAAGAACCCCTAGATCGCCGCTGAAATCAATGGCCGCAGAGTTCCCGGAGCTTCGAGGAATGTGCAGGAATCTCGTCGCTCGGATCGGCAGGTCTCGCCGCCGGTACCATCACTCCGCCGATTTCCGATACGATCCTTCTAGCTATGCGCTTAATTTCGAGGACGAACAGGTTCGGGATGATGATGAGTTTCCGATTAGGGATTTCGCTTCCAGATTGCCGGCGTCTCCGCCGACTTCCCCGAGACTTTCGCCCGCGAAATGA

Coding sequence (CDS)

ATGATGTTGAACGACTACCATCCATTTTCATTTACTCGCAAGCTCAAATCCTCAATCCTCTGTTTCCGTCGCGATCATGGCGATTTCCTCGAAAACGAAGGCGATTCTTCTCCAAGAACCCCTAGATCGCCGCTGAAATCAATGGCCGCAGAGTTCCCGGAGCTTCGAGGAATGTGCAGGAATCTCGTCGCTCGGATCGGCAGGTCTCGCCGCCGGTACCATCACTCCGCCGATTTCCGATACGATCCTTCTAGCTATGCGCTTAATTTCGAGGACGAACAGGTTCGGGATGATGATGAGTTTCCGATTAGGGATTTCGCTTCCAGATTGCCGGCGTCTCCGCCGACTTCCCCGAGACTTTCGCCCGCGAAATGA

Protein sequence

MMLNDYHPFSFTRKLKSSILCFRRDHGDFLENEGDSSPRTPRSPLKSMAAEFPELRGMCRNLVARIGRSRRRYHHSADFRYDPSSYALNFEDEQVRDDDEFPIRDFASRLPASPPTSPRLSPAK
Homology
BLAST of CmoCh01G015400 vs. ExPASy TrEMBL
Match: A0A6J1FP70 (uncharacterized protein LOC111446022 OS=Cucurbita moschata OX=3662 GN=LOC111446022 PE=4 SV=1)

HSP 1 Score: 255.4 bits (651), Expect = 1.2e-64
Identity = 124/124 (100.00%), Postives = 124/124 (100.00%), Query Frame = 0

Query: 1   MMLNDYHPFSFTRKLKSSILCFRRDHGDFLENEGDSSPRTPRSPLKSMAAEFPELRGMCR 60
           MMLNDYHPFSFTRKLKSSILCFRRDHGDFLENEGDSSPRTPRSPLKSMAAEFPELRGMCR
Sbjct: 1   MMLNDYHPFSFTRKLKSSILCFRRDHGDFLENEGDSSPRTPRSPLKSMAAEFPELRGMCR 60

Query: 61  NLVARIGRSRRRYHHSADFRYDPSSYALNFEDEQVRDDDEFPIRDFASRLPASPPTSPRL 120
           NLVARIGRSRRRYHHSADFRYDPSSYALNFEDEQVRDDDEFPIRDFASRLPASPPTSPRL
Sbjct: 61  NLVARIGRSRRRYHHSADFRYDPSSYALNFEDEQVRDDDEFPIRDFASRLPASPPTSPRL 120

Query: 121 SPAK 125
           SPAK
Sbjct: 121 SPAK 124

BLAST of CmoCh01G015400 vs. ExPASy TrEMBL
Match: A0A6J1J1H3 (uncharacterized protein LOC111480417 OS=Cucurbita maxima OX=3661 GN=LOC111480417 PE=4 SV=1)

HSP 1 Score: 236.9 bits (603), Expect = 4.6e-59
Identity = 115/124 (92.74%), Postives = 118/124 (95.16%), Query Frame = 0

Query: 1   MMLNDYHPFSFTRKLKSSILCFRRDHGDFLENEGDSSPRTPRSPLKSMAAEFPELRGMCR 60
           MMLN+YHPFSFTRKLKSSI CFRRDH DF E+E DSSPRTPRSPLKSMAAEFPE+RGMCR
Sbjct: 1   MMLNEYHPFSFTRKLKSSIRCFRRDHSDFFESEDDSSPRTPRSPLKSMAAEFPEIRGMCR 60

Query: 61  NLVARIGRSRRRYHHSADFRYDPSSYALNFEDEQVRDDDEFPIRDFASRLPASPPTSPRL 120
           NLVARIGR RRRYHHSADFRYDPSSYALNFEDEQVRDDDEFPIRDFASRLPASPPTS RL
Sbjct: 61  NLVARIGRCRRRYHHSADFRYDPSSYALNFEDEQVRDDDEFPIRDFASRLPASPPTSRRL 120

Query: 121 SPAK 125
           SPAK
Sbjct: 121 SPAK 124

BLAST of CmoCh01G015400 vs. ExPASy TrEMBL
Match: A0A6J1CC83 (uncharacterized protein LOC111009386 OS=Momordica charantia OX=3673 GN=LOC111009386 PE=4 SV=1)

HSP 1 Score: 190.3 bits (482), Expect = 4.9e-45
Identity = 96/126 (76.19%), Postives = 107/126 (84.92%), Query Frame = 0

Query: 1   MMLNDYH--PFSFTRKLKSSILCFRRDHGDFLENEGDSSPRTPRSPLKSMAAEFPELRGM 60
           M+ N+Y+  PFSFT KLKSSILCFR DHGD  ++E ++S R+PRSPLKS A+E  ELRGM
Sbjct: 1   MLNNEYNSSPFSFTHKLKSSILCFRADHGDLGDHEDEASLRSPRSPLKSTASEHTELRGM 60

Query: 61  CRNLVARIGRSRRRYHHSADFRYDPSSYALNFEDEQVRDDDEFPIRDFASRLPASPPTSP 120
           CRNLVARIGRSRRRYHHSADFRYDPSSYALNFEDE  R DD+FPIRDFASRLPASP +SP
Sbjct: 61  CRNLVARIGRSRRRYHHSADFRYDPSSYALNFEDEHARYDDDFPIRDFASRLPASPHSSP 120

Query: 121 RLSPAK 125
              PAK
Sbjct: 121 IFQPAK 126

BLAST of CmoCh01G015400 vs. ExPASy TrEMBL
Match: A0A5A7VBS3 (DNA ligase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold134G00970 PE=4 SV=1)

HSP 1 Score: 157.5 bits (397), Expect = 3.5e-35
Identity = 84/116 (72.41%), Postives = 90/116 (77.59%), Query Frame = 0

Query: 2   MLNDYHPFSFTRKLKSSI-LCFRRDHGDFLENEGDSSPRTPRSPLKSMAAEFPELRGMCR 61
           M N Y+PFSFT KLKSSI  CFR DH   L    D +P      LKSM ++ PELRGMCR
Sbjct: 1   MFNHYNPFSFTHKLKSSIPSCFRPDHDAELHENEDDNP-----ALKSMTSDLPELRGMCR 60

Query: 62  NLVARIG-RSRRRYHHSADFRYDPSSYALNFEDEQVRDDDEFPIRDFASRLPASPP 116
           +LVARI   SRRRYHHSADFRYDPSSYALNFEDEQVRDD+EFPIRDF SRLPASPP
Sbjct: 61  SLVARISWPSRRRYHHSADFRYDPSSYALNFEDEQVRDDNEFPIRDFTSRLPASPP 111

BLAST of CmoCh01G015400 vs. ExPASy TrEMBL
Match: A0A0A0L4E2 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G652740 PE=4 SV=1)

HSP 1 Score: 157.5 bits (397), Expect = 3.5e-35
Identity = 83/116 (71.55%), Postives = 91/116 (78.45%), Query Frame = 0

Query: 2   MLNDYHPFSFTRKLKSSILCFRRDHGDFLENEGDSSPRTPRSP-LKSMAAEFPELRGMCR 61
           M N Y+PFSFT KLKS I CFR DH    + E   +P +P SP LKSM ++ PELRGMCR
Sbjct: 1   MFNHYNPFSFTHKLKSPISCFRPDH----DPELHDNPSSPTSPALKSMTSDLPELRGMCR 60

Query: 62  NLVARIG-RSRRRYHHSADFRYDPSSYALNFEDEQVRDDDEFPIRDFASRLPASPP 116
           +LVARI   SRRRYHHS DFRYDPSSYALNFEDEQ+R DDEFPIRDF SRLPASPP
Sbjct: 61  SLVARISWPSRRRYHHSTDFRYDPSSYALNFEDEQLRYDDEFPIRDFTSRLPASPP 112

BLAST of CmoCh01G015400 vs. NCBI nr
Match: XP_022940408.1 (uncharacterized protein LOC111446022 [Cucurbita moschata] >KAG6608139.1 hypothetical protein SDJN03_01481, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 255.4 bits (651), Expect = 2.6e-64
Identity = 124/124 (100.00%), Postives = 124/124 (100.00%), Query Frame = 0

Query: 1   MMLNDYHPFSFTRKLKSSILCFRRDHGDFLENEGDSSPRTPRSPLKSMAAEFPELRGMCR 60
           MMLNDYHPFSFTRKLKSSILCFRRDHGDFLENEGDSSPRTPRSPLKSMAAEFPELRGMCR
Sbjct: 1   MMLNDYHPFSFTRKLKSSILCFRRDHGDFLENEGDSSPRTPRSPLKSMAAEFPELRGMCR 60

Query: 61  NLVARIGRSRRRYHHSADFRYDPSSYALNFEDEQVRDDDEFPIRDFASRLPASPPTSPRL 120
           NLVARIGRSRRRYHHSADFRYDPSSYALNFEDEQVRDDDEFPIRDFASRLPASPPTSPRL
Sbjct: 61  NLVARIGRSRRRYHHSADFRYDPSSYALNFEDEQVRDDDEFPIRDFASRLPASPPTSPRL 120

Query: 121 SPAK 125
           SPAK
Sbjct: 121 SPAK 124

BLAST of CmoCh01G015400 vs. NCBI nr
Match: KAG7031776.1 (hypothetical protein SDJN02_05817, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 253.4 bits (646), Expect = 9.8e-64
Identity = 123/123 (100.00%), Postives = 123/123 (100.00%), Query Frame = 0

Query: 2   MLNDYHPFSFTRKLKSSILCFRRDHGDFLENEGDSSPRTPRSPLKSMAAEFPELRGMCRN 61
           MLNDYHPFSFTRKLKSSILCFRRDHGDFLENEGDSSPRTPRSPLKSMAAEFPELRGMCRN
Sbjct: 1   MLNDYHPFSFTRKLKSSILCFRRDHGDFLENEGDSSPRTPRSPLKSMAAEFPELRGMCRN 60

Query: 62  LVARIGRSRRRYHHSADFRYDPSSYALNFEDEQVRDDDEFPIRDFASRLPASPPTSPRLS 121
           LVARIGRSRRRYHHSADFRYDPSSYALNFEDEQVRDDDEFPIRDFASRLPASPPTSPRLS
Sbjct: 61  LVARIGRSRRRYHHSADFRYDPSSYALNFEDEQVRDDDEFPIRDFASRLPASPPTSPRLS 120

Query: 122 PAK 125
           PAK
Sbjct: 121 PAK 123

BLAST of CmoCh01G015400 vs. NCBI nr
Match: XP_023523424.1 (uncharacterized protein LOC111787637 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 247.3 bits (630), Expect = 7.0e-62
Identity = 121/123 (98.37%), Postives = 121/123 (98.37%), Query Frame = 0

Query: 2   MLNDYHPFSFTRKLKSSILCFRRDHGDFLENEGDSSPRTPRSPLKSMAAEFPELRGMCRN 61
           MLNDYHPFSFTRKLKSSILCFRRDHGDFLENE DSSPRTPRSPLKSMAAEFPELRGMCRN
Sbjct: 1   MLNDYHPFSFTRKLKSSILCFRRDHGDFLENEDDSSPRTPRSPLKSMAAEFPELRGMCRN 60

Query: 62  LVARIGRSRRRYHHSADFRYDPSSYALNFEDEQVRDDDEFPIRDFASRLPASPPTSPRLS 121
           LVARIGRSRRRYHHSADFRYDPSSYALNFEDEQVRDDDEFPIRDFASRLPASPPTS RLS
Sbjct: 61  LVARIGRSRRRYHHSADFRYDPSSYALNFEDEQVRDDDEFPIRDFASRLPASPPTSRRLS 120

Query: 122 PAK 125
           PAK
Sbjct: 121 PAK 123

BLAST of CmoCh01G015400 vs. NCBI nr
Match: XP_022981208.1 (uncharacterized protein LOC111480417 [Cucurbita maxima])

HSP 1 Score: 236.9 bits (603), Expect = 9.5e-59
Identity = 115/124 (92.74%), Postives = 118/124 (95.16%), Query Frame = 0

Query: 1   MMLNDYHPFSFTRKLKSSILCFRRDHGDFLENEGDSSPRTPRSPLKSMAAEFPELRGMCR 60
           MMLN+YHPFSFTRKLKSSI CFRRDH DF E+E DSSPRTPRSPLKSMAAEFPE+RGMCR
Sbjct: 1   MMLNEYHPFSFTRKLKSSIRCFRRDHSDFFESEDDSSPRTPRSPLKSMAAEFPEIRGMCR 60

Query: 61  NLVARIGRSRRRYHHSADFRYDPSSYALNFEDEQVRDDDEFPIRDFASRLPASPPTSPRL 120
           NLVARIGR RRRYHHSADFRYDPSSYALNFEDEQVRDDDEFPIRDFASRLPASPPTS RL
Sbjct: 61  NLVARIGRCRRRYHHSADFRYDPSSYALNFEDEQVRDDDEFPIRDFASRLPASPPTSRRL 120

Query: 121 SPAK 125
           SPAK
Sbjct: 121 SPAK 124

BLAST of CmoCh01G015400 vs. NCBI nr
Match: XP_022138148.1 (uncharacterized protein LOC111009386 [Momordica charantia])

HSP 1 Score: 190.3 bits (482), Expect = 1.0e-44
Identity = 96/126 (76.19%), Postives = 107/126 (84.92%), Query Frame = 0

Query: 1   MMLNDYH--PFSFTRKLKSSILCFRRDHGDFLENEGDSSPRTPRSPLKSMAAEFPELRGM 60
           M+ N+Y+  PFSFT KLKSSILCFR DHGD  ++E ++S R+PRSPLKS A+E  ELRGM
Sbjct: 1   MLNNEYNSSPFSFTHKLKSSILCFRADHGDLGDHEDEASLRSPRSPLKSTASEHTELRGM 60

Query: 61  CRNLVARIGRSRRRYHHSADFRYDPSSYALNFEDEQVRDDDEFPIRDFASRLPASPPTSP 120
           CRNLVARIGRSRRRYHHSADFRYDPSSYALNFEDE  R DD+FPIRDFASRLPASP +SP
Sbjct: 61  CRNLVARIGRSRRRYHHSADFRYDPSSYALNFEDEHARYDDDFPIRDFASRLPASPHSSP 120

Query: 121 RLSPAK 125
              PAK
Sbjct: 121 IFQPAK 126

BLAST of CmoCh01G015400 vs. TAIR 10
Match: AT5G35090.1 (unknown protein; Has 1807 Blast hits to 1807 proteins in 277 species: Archae - 0; Bacteria - 0; Metazoa - 736; Fungi - 347; Plants - 385; Viruses - 0; Other Eukaryotes - 339 (source: NCBI BLink). )

HSP 1 Score: 65.9 bits (159), Expect = 2.7e-11
Identity = 48/137 (35.04%), Postives = 68/137 (49.64%), Query Frame = 0

Query: 10  SFTRKLKSSIL---CFRRDHGDFLENEGDSSPRTPRSPLKS--------MAAEFPEL--- 69
           S  +KLKS      CFR  +    +N  D  P +P +  KS        M  + P L   
Sbjct: 10  SLQKKLKSRFCIAGCFRTTNHHHHDNIPDDLPSSPVTTEKSTQSPHGGGMKTKSPRLTRT 69

Query: 70  ----RGMCRNLVARI--------GRSRRRYHHSADFRYDPSSYALNFEDEQVRDD-DEFP 120
                  C++L+ R+        G  +    H+ADF YDPSSYALNF+     D+ D FP
Sbjct: 70  LSKSHEKCKSLIHRMGGGVGGVGGHGKHIRRHTADFHYDPSSYALNFDKGDEDDNIDRFP 129

BLAST of CmoCh01G015400 vs. TAIR 10
Match: AT5G11070.1 (unknown protein; Has 1807 Blast hits to 1807 proteins in 277 species: Archae - 0; Bacteria - 0; Metazoa - 736; Fungi - 347; Plants - 385; Viruses - 0; Other Eukaryotes - 339 (source: NCBI BLink). )

HSP 1 Score: 62.8 bits (151), Expect = 2.3e-10
Identity = 47/99 (47.47%), Postives = 54/99 (54.55%), Query Frame = 0

Query: 39  RTPRSP---LKSMAAEFPELRGMCRNLVARIGRSRR-----------RYHHS----ADFR 98
           RTPRSP   LKS A E  ELR  CR + +RI  + R            +HHS     DF 
Sbjct: 47  RTPRSPYEWLKSTAQEL-ELRDRCRRVKSRIKVTCRNNNCAYNNCVHHHHHSQSYPGDFS 106

Query: 99  YDPSSYALNFEDEQVRDDDEFPIRDFASRLPASPPTSPR 120
           YDP SYALNFED  VR DD+    +F +RLP SP T  R
Sbjct: 107 YDPLSYALNFED-NVRADDDGSFPNFTARLPQSPVTKTR 143

BLAST of CmoCh01G015400 vs. TAIR 10
Match: AT3G48020.1 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: chloroplast; EXPRESSED IN: 11 plant structures; EXPRESSED DURING: LP.04 four leaves visible, 4 anthesis; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT5G62865.1); Has 82 Blast hits to 82 proteins in 12 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 82; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink). )

HSP 1 Score: 41.6 bits (96), Expect = 5.4e-04
Identity = 30/70 (42.86%), Postives = 37/70 (52.86%), Query Frame = 0

Query: 60  RNLVARIGRSRRR---YHHSADFRYDPSSYALNFEDEQVRDDDEF---PIRDFASRLPAS 119
           +  + R  R  RR   +  S  FRYDP SY L+FEDE   DDDE     +R F+ R  AS
Sbjct: 59  KTFIRRFNRDPRRGQDWDDSDKFRYDPVSYTLSFEDEDKDDDDEAGVGGVRSFSMRY-AS 118

Query: 120 PPTSPRLSPA 124
            P +   SPA
Sbjct: 119 VPVASGKSPA 127

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A6J1FP701.2e-64100.00uncharacterized protein LOC111446022 OS=Cucurbita moschata OX=3662 GN=LOC1114460... [more]
A0A6J1J1H34.6e-5992.74uncharacterized protein LOC111480417 OS=Cucurbita maxima OX=3661 GN=LOC111480417... [more]
A0A6J1CC834.9e-4576.19uncharacterized protein LOC111009386 OS=Momordica charantia OX=3673 GN=LOC111009... [more]
A0A5A7VBS33.5e-3572.41DNA ligase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold134G00970 PE=... [more]
A0A0A0L4E23.5e-3571.55Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G652740 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
XP_022940408.12.6e-64100.00uncharacterized protein LOC111446022 [Cucurbita moschata] >KAG6608139.1 hypothet... [more]
KAG7031776.19.8e-64100.00hypothetical protein SDJN02_05817, partial [Cucurbita argyrosperma subsp. argyro... [more]
XP_023523424.17.0e-6298.37uncharacterized protein LOC111787637 [Cucurbita pepo subsp. pepo][more]
XP_022981208.19.5e-5992.74uncharacterized protein LOC111480417 [Cucurbita maxima][more]
XP_022138148.11.0e-4476.19uncharacterized protein LOC111009386 [Momordica charantia][more]
Match NameE-valueIdentityDescription
AT5G35090.12.7e-1135.04unknown protein; Has 1807 Blast hits to 1807 proteins in 277 species: Archae - 0... [more]
AT5G11070.12.3e-1047.47unknown protein; Has 1807 Blast hits to 1807 proteins in 277 species: Archae - 0... [more]
AT3G48020.15.4e-0442.86unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biologic... [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 101..124
NoneNo IPR availablePANTHERPTHR33168STRESS INDUCED PROTEIN-RELATEDcoord: 14..118
NoneNo IPR availablePANTHERPTHR33168:SF61SUBFAMILY NOT NAMEDcoord: 14..118

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh01G015400.1CmoCh01G015400.1mRNA