CmoCh01G013500 (gene) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh01G013500
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
Descriptioncholine-phosphate cytidylyltransferase 2-like
LocationCmo_Chr01: 10688230 .. 10692286 (-)
RNA-Seq ExpressionCmoCh01G013500
SyntenyCmoCh01G013500
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATAAGAAAATAAAAGAAACAAATAAATAAGGAGCGGAAACATCGCGTTGTCGGAGGAGAAGAAATCGACAACAAGAGGGCTCGCCGAAGCAGAAGAACGGAAGCGAAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAAAGAAGAGGTCGGAAGATAATTCGAATGGCAGAGGTGAAGCCCGCCGATTCCACCACCACTATCAACGCTTCTCCTAACCATTCCAATCCTGATTACCCGACCGACCGCCCAGTTCGAGTTTACGCCGATGGGATCTACGATCTCTTCCATTTCGGTCACGCTCGCTCTCTCGAGCAAGCCAAGAAATCGTCCGTTTCTTCTCCTTTTCCTTAATTTCTCTTCGCTTTCTTGGTTATTCTTCATGTGCGGCATCTGTGACTGTAGTTTCCATGATTTTTTTGGTTTCTGATACTTTTCGATTTAAGTAATTTAGTTCCTGTGTTTCGCCGGGTCGTAGGAATTGTTGTTTTCTCTTATTGTGATTTTTTTTTCTTTTTCTGATTCATTAGCCTGCTTGATTGTTCTTAATTTTCAGGTTTGCTAACTTGTTTTCACTTGTCCGTGAACTTCTTCTGTTGGGAGAATTGATTTGTTGAATTGACGAGCTGTAATTATTGATCCTTCTTGTAATTTCAGATTCCCGAATACTTATTTGTTAGTTGGATGTTGCAATGATGAGACTACCCACAGTCTTAAAGGGAAAACTGTTATGAATGAAGCTGAACGATATGAATCTCTTCGCCATTGCAAGTATGACTTTTCTCTATTCCATGTATTTTTCTTTACTGGGGCTTTTGAATCTTGAAACATTGATATGTATAGATGTGCTTTTGATACCTACAAACGCTAGTTAGAATGCCTGTTTGAATCCTTTAAATTCAAGTGTAGCTATGATGGACGTGGAATCAATCTTAGTCGCACCGATTCTTCAACGTGATTTTATTTATTTGCTCCTTGTTTCCCCCTCCCTTTTCCGCAGGTGGGTGGATGAAGTGATTCCTGATGCACCCTGGGTGATCTCTCAAGAATTTATTGACAAGCACAAGATTGACTTTGTGGCCCACGATTCTCTTCCGTAAGGATATTATATACTTTATCTCAGTGAACCAAGAGGGTCAAGTAACTTTTGGGCAATGGAGTTTATTTCCTTTTCCACATTTATCTGCATTTCTACCCATCTAATTAGTATAGATTGGATGGTAAAGTGTCTCAGGAAGAGCTTCAATTAGTTGGTCCATGGTATCTATCTGTTCACATTGAATTTATTTGTTAATTCTGTTTTAAATGACCTATGTCATCCTTTCTCTGCCTCGATACAGTTATGCTGATGCTACCGGAGCTGGGAAGGATGTTTATGAATTTGTAAGTTCAGAAGATGGGTTTATCCATTCGATCCACTTGGTGCTTAAGAACATACAATTTCATAGATGGGAACTTTTCCTTTGTAGGTTAAAAAAATTGGAAAGTTCAAGGAAACAAAAAGGACAGAAGGAATATCAACTTCAGACATTATCATGAGGATTGTCAAAGATTATAATCAATATGTTCTGCGTAATTTGGACCGTGGTTATTCGAGGAAAGAGCTTGGAGTCAGCTTTGTGAAGGCATGATATTTTATCTACATTGACCCTTTTTATAACGATAATGCTTATCCTGCTAATCAGCAAAGCTTGATTATATTGATTTTGTTTATTAACAAGCAACTCAATCTTGATGTATTCAATTACCAGGAAAAGCGGTTAAGAGTGAATATGAGATTGAAGAAATTACAGGAGAAAGTGAAGGAACATCAAGAAAAAGTGGGAGAAAAGGTACCCATATCTCATATCTTTATAATAGTCCTATGGATGTGGTAGTTGGGGAGTTCAGATTAGATTATCTGCTTTATAATTCCTCTTATTTCAGGCTATGGGATAGTTGTACCTTAGGTGTAACAAACTTTCTATATGCACAAAAGCTGGTCCGAACACTTACTAATATAACAAAAAAAAGAAAAAAAAAAAGAAAGAAAGAGGAAGCTGGGCAGTCCACTTGCAATCTTGCAAATTTGTTACTAGAATTTGCCTAAAGGCGTGTCTTATGTTTTGTGCATTTTTGCTATGACTTGATAGCTAACCATAAGTTTCAGGAAAAAAGCTAGAGATCTTCACATTAGAGAAAGTCAATATTATTCGATGAAATCCATATTTCTACGGTTTTATTTTTAATAAACTTAGCTATGCACTGTGTGCTTTTGGGCCCACCTTTTTCACCTTGCCTGGTATGTGCATCTTAAGATGCCTAAATGTTTTTACGACCCATAATTATTTGGAACGATCAGATACTTCACTGTCCTTGCACATATGCGAATATGATTTCGTGACCAATTGAAGAACTTAGATGAGATGATTCTATGCTACATTTTGTATCTTCCTGAAATGACTTGAATTAAGACACTAAAAGAGCAAAACACGAAGGAACAACTTGTTGTAAATGTATCTATGGGGTTTGTCTGCCTTCAGTTGATTTTCGAACTTACTCCAAAAATAATGTGGTTTGTGCGGCTGCCTTTCACATTTCTCTCTGTTTTATTTCATTTTGTGATATATTTTCTTAGAAAAGGATTCTAATTGTACATTCATGAAGTTGTCTTTTCAAGTAAGAAAAGTTGACTTTTGAGCCAGCTATCTGCCATGCAAGCATTACAATTCTTTTGGGTTCTTTTTTTGAGCATGTGAAACGATGCCAGATACAGCTGTGCTGATATCAAAGCATGGAATCCTAGTATTTCAATGTATTCACAGACATATTCTTGCTTTTCAATTCTTTCTTAAGCTTGGAAATCCCAATTTTTGTTTTTGTACTAATTCAAGCATTGAAGTTTTTATGGGTAATTGATATTTAGCCCGCCTAATATAGCAAGTTGATTACTTCTTGGGTGTATTCTATTCTCCCCATTGAAGTGAAAAAAGTCCTTTTTTCCACTTCAGTGCTAGCGAGGACTTTATCTGGTTTTGTTAGACCTACAATTATATTTTAGGCTTTATTTACCTCGATCTAATTATGGAGGCAGGATATTTAATTATTGTTTTTTGTTGAAAATGATTGTTCTGGATACTGCAGATTCAAACAGTGGCAAAAACTGCCGGCATGCACAGGAATGAATGGGTGGAAAATGCTGATCGCTGGGTGGCTGGATTTCTAGAGATGTTTGAAGAAGGTTGTCATAAGATGGTAAGTTAATGCACGGATTATCCTGTTCTTCATTTATGTGGCAAGTTTTGAAGTAGATAAGAAAAAGAAAGCATACAAAAACGTACATGGTTTTGTGAATCTTGAACTAAAACAAAAGGGTTAAATCTGAAACATCACGTGTGTATTTAGGGCACGGCCATCAGAGACCGAATTCAAGAGCGGTTAATGGGGGCACAGCAGCAAAAATATCTAGAAAATGGCAAGGACAGTGACTCAGACCTCGAAGAAGAATATTATTACGACGACGACGACGAAGAATATTACGAAGACGACTTCTACGAAGAATTCGAGAAAGACGAGAAGAAAAGGTAGACACGTATTTACATCAAGTTTGAATACATGTCACATGCATAATTTGATGCTTGTTCATGGATTCTACAGTAGTAGTATGTTTCTGCTATATGTTTAGGCGCAGACAGTAAACGTAAACCATAACAGCTAATTTGCCAAAGCAAAAAAGGAAAACAAAAGAGGGGCACTTGGCGGTGAGTTCAGGCACCAATGTGGCTTTTGTGACCCTTTTTAAGATATGTGTTTGTGCATATAGGTTGAAATTTTTTCCCTTGAAGCTTGTAAAACAGGTCGTTTCTTTTCGGGTTTTGTTTGTGAATCGTCGTAATTGAAGAGTTTTGAAGCTTTGGGTTTCCTGTTTGTATGTAATGTAAGAGTGATAAATATGGTTTGATATTATTATATGTATCTTTCTCTTTGTTTTTGGTCATTCTGTAAAGGAGAATTGAAAGGGAATTTGTGAAAAGCATAAGATTAGTGGGATACTTGAAAGGAGGTTGTTTGCTTACCTAA

mRNA sequence

ATAAGAAAATAAAAGAAACAAATAAATAAGGAGCGGAAACATCGCGTTGTCGGAGGAGAAGAAATCGACAACAAGAGGGCTCGCCGAAGCAGAAGAACGGAAGCGAAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAAAGAAGAGGTCGGAAGATAATTCGAATGGCAGAGGTGAAGCCCGCCGATTCCACCACCACTATCAACGCTTCTCCTAACCATTCCAATCCTGATTACCCGACCGACCGCCCAGTTCGAGTTTACGCCGATGGGATCTACGATCTCTTCCATTTCGGTCACGCTCGCTCTCTCGAGCAAGCCAAGAAATCATTCCCGAATACTTATTTGTTAGTTGGATGTTGCAATGATGAGACTACCCACAGTCTTAAAGGGAAAACTGTTATGAATGAAGCTGAACGATATGAATCTCTTCGCCATTGCAAGTGGGTGGATGAAGTGATTCCTGATGCACCCTGGGTGATCTCTCAAGAATTTATTGACAAGCACAAGATTGACTTTGTGGCCCACGATTCTCTTCCTTATGCTGATGCTACCGGAGCTGGGAAGGATGTTTATGAATTTGTTAAAAAAATTGGAAAGTTCAAGGAAACAAAAAGGACAGAAGGAATATCAACTTCAGACATTATCATGAGGATTGTCAAAGATTATAATCAATATGTTCTGCGTAATTTGGACCGTGGTTATTCGAGGAAAGAGCTTGGAGTCAGCTTTCAACTCAATCTTGATGTATTCAATTACCAGGAAAAGCGGTTAAGAGTGAATATGAGATTGAAGAAATTACAGGAGAAAGTGAAGGAACATCAAGAAAAAGTGGGAGAAAAGATTCAAACAGTGGCAAAAACTGCCGGCATGCACAGGAATGAATGGGTGGAAAATGCTGATCGCTGGGTGGCTGGATTTCTAGAGATGTTTGAAGAAGGTTGTCATAAGATGGGCACGGCCATCAGAGACCGAATTCAAGAGCGGTTAATGGGGGCACAGCAGCAAAAATATCTAGAAAATGGCAAGGACAGTGACTCAGACCTCGAAGAAGAATATTATTACGACGACGACGACGAAGAATATTACGAAGACGACTTCTACGAAGAATTCGAGAAAGACGAGAAGAAAAGGAGAATTGAAAGGGAATTTGTGAAAAGCATAAGATTAGTGGGATACTTGAAAGGAGGTTGTTTGCTTACCTAA

Coding sequence (CDS)

ATGGCAGAGGTGAAGCCCGCCGATTCCACCACCACTATCAACGCTTCTCCTAACCATTCCAATCCTGATTACCCGACCGACCGCCCAGTTCGAGTTTACGCCGATGGGATCTACGATCTCTTCCATTTCGGTCACGCTCGCTCTCTCGAGCAAGCCAAGAAATCATTCCCGAATACTTATTTGTTAGTTGGATGTTGCAATGATGAGACTACCCACAGTCTTAAAGGGAAAACTGTTATGAATGAAGCTGAACGATATGAATCTCTTCGCCATTGCAAGTGGGTGGATGAAGTGATTCCTGATGCACCCTGGGTGATCTCTCAAGAATTTATTGACAAGCACAAGATTGACTTTGTGGCCCACGATTCTCTTCCTTATGCTGATGCTACCGGAGCTGGGAAGGATGTTTATGAATTTGTTAAAAAAATTGGAAAGTTCAAGGAAACAAAAAGGACAGAAGGAATATCAACTTCAGACATTATCATGAGGATTGTCAAAGATTATAATCAATATGTTCTGCGTAATTTGGACCGTGGTTATTCGAGGAAAGAGCTTGGAGTCAGCTTTCAACTCAATCTTGATGTATTCAATTACCAGGAAAAGCGGTTAAGAGTGAATATGAGATTGAAGAAATTACAGGAGAAAGTGAAGGAACATCAAGAAAAAGTGGGAGAAAAGATTCAAACAGTGGCAAAAACTGCCGGCATGCACAGGAATGAATGGGTGGAAAATGCTGATCGCTGGGTGGCTGGATTTCTAGAGATGTTTGAAGAAGGTTGTCATAAGATGGGCACGGCCATCAGAGACCGAATTCAAGAGCGGTTAATGGGGGCACAGCAGCAAAAATATCTAGAAAATGGCAAGGACAGTGACTCAGACCTCGAAGAAGAATATTATTACGACGACGACGACGAAGAATATTACGAAGACGACTTCTACGAAGAATTCGAGAAAGACGAGAAGAAAAGGAGAATTGAAAGGGAATTTGTGAAAAGCATAAGATTAGTGGGATACTTGAAAGGAGGTTGTTTGCTTACCTAA

Protein sequence

MAEVKPADSTTTINASPNHSNPDYPTDRPVRVYADGIYDLFHFGHARSLEQAKKSFPNTYLLVGCCNDETTHSLKGKTVMNEAERYESLRHCKWVDEVIPDAPWVISQEFIDKHKIDFVAHDSLPYADATGAGKDVYEFVKKIGKFKETKRTEGISTSDIIMRIVKDYNQYVLRNLDRGYSRKELGVSFQLNLDVFNYQEKRLRVNMRLKKLQEKVKEHQEKVGEKIQTVAKTAGMHRNEWVENADRWVAGFLEMFEEGCHKMGTAIRDRIQERLMGAQQQKYLENGKDSDSDLEEEYYYDDDDEEYYEDDFYEEFEKDEKKRRIEREFVKSIRLVGYLKGGCLLT
Homology
BLAST of CmoCh01G013500 vs. ExPASy Swiss-Prot
Match: Q9ZV56 (Choline-phosphate cytidylyltransferase 1 OS=Arabidopsis thaliana OX=3702 GN=CCT1 PE=1 SV=1)

HSP 1 Score: 439.1 bits (1128), Expect = 4.6e-122
Identity = 230/323 (71.21%), Postives = 266/323 (82.35%), Query Frame = 0

Query: 7   ADSTTTINASPNHSNPDYPTDRPVRVYADGIYDLFHFGHARSLEQAKKSFP-NTYLLVGC 66
           A  +T + +SP       PTDRPVRVYADGIYDLFHFGHARSLEQAK +FP NTYLLVGC
Sbjct: 19  ASGSTAVQSSP-------PTDRPVRVYADGIYDLFHFGHARSLEQAKLAFPNNTYLLVGC 78

Query: 67  CNDETTHSLKGKTVMNEAERYESLRHCKWVDEVIPDAPWVISQEFIDKHKIDFVAHDSLP 126
           CNDETTH  KG+TVM   ERYESLRHCKWVDEVIPDAPWV++QEF+DKH+ID+VAHDSLP
Sbjct: 79  CNDETTHKYKGRTVMTAEERYESLRHCKWVDEVIPDAPWVVNQEFLDKHQIDYVAHDSLP 138

Query: 127 YADATGAGKDVYEFVKKIGKFKETKRTEGISTSDIIMRIVKDYNQYVLRNLDRGYSRKEL 186
           YAD++GAGKDVYEFVKK+G+FKET+RTEGISTSDIIMRIVKDYNQYV+RNLDRGYSR++L
Sbjct: 139 YADSSGAGKDVYEFVKKVGRFKETQRTEGISTSDIIMRIVKDYNQYVMRNLDRGYSREDL 198

Query: 187 GVSFQLNLDVFNYQEKRLRVNMRLKKLQEKVKEHQEKVGEKIQTVAKTAGMHRNEWVENA 246
           GVSF         +EKRLRVNMRLKKLQE+VKE QE+VGEKIQTV     M RNEWVENA
Sbjct: 199 GVSF--------VKEKRLRVNMRLKKLQERVKEQQERVGEKIQTVK----MLRNEWVENA 258

Query: 247 DRWVAGFLEMFEEGCHKMGTAIRDRIQERLMGAQQQKYLENGKDSDSD--LEEEYY---- 306
           DRWVAGFLE+FEEGCHKMGTAI D IQERLM  +  + LENG+D D+D    EEY+    
Sbjct: 259 DRWVAGFLEIFEEGCHKMGTAIVDSIQERLMRQKSAERLENGQDDDTDDQFYEEYFDHDM 318

Query: 307 --YDDDDEEYYEDDFYEEFEKDE 321
              DD+DE++Y+++  +E E ++
Sbjct: 319 GSDDDEDEKFYDEEEVKEEETEK 322

BLAST of CmoCh01G013500 vs. ExPASy Swiss-Prot
Match: F4JJE0 (Choline-phosphate cytidylyltransferase 2 OS=Arabidopsis thaliana OX=3702 GN=CCT2 PE=1 SV=1)

HSP 1 Score: 433.3 bits (1113), Expect = 2.5e-120
Identity = 220/289 (76.12%), Postives = 245/289 (84.78%), Query Frame = 0

Query: 26  TDRPVRVYADGIYDLFHFGHARSLEQAKKSFPNTYLLVGCCNDETTHSLKGKTVMNEAER 85
           +DRPVRVYADGI+DLFHFGHAR++EQAKKSFPNTYLLVGCCNDE T+  KGKTVM E+ER
Sbjct: 17  SDRPVRVYADGIFDLFHFGHARAIEQAKKSFPNTYLLVGCCNDEITNKFKGKTVMTESER 76

Query: 86  YESLRHCKWVDEVIPDAPWVISQEFIDKHKIDFVAHDSLPYADATGAGKDVYEFVKKIGK 145
           YESLRHCKWVDEVIPDAPWV++ EF+DKHKID+VAHD+LPYAD +GAG DVYEFVK IGK
Sbjct: 77  YESLRHCKWVDEVIPDAPWVLTTEFLDKHKIDYVAHDALPYADTSGAGNDVYEFVKSIGK 136

Query: 146 FKETKRTEGISTSDIIMRIVKDYNQYVLRNLDRGYSRKELGVSFQLNLDVFNYQEKRLRV 205
           FKETKRTEGISTSDIIMRIVKDYNQYVLRNLDRGYSR+ELGVSF         +EKRLRV
Sbjct: 137 FKETKRTEGISTSDIIMRIVKDYNQYVLRNLDRGYSREELGVSF---------EEKRLRV 196

Query: 206 NMRLKKLQEKVKEHQEKVGEKIQTVAKTAGMHRNEWVENADRWVAGFLEMFEEGCHKMGT 265
           NMRLKKLQEKVKE Q    EKIQTVAKTAGMH +EW+ENADRWVAGFLEMFEEGCHKMGT
Sbjct: 197 NMRLKKLQEKVKEQQ----EKIQTVAKTAGMHHDEWLENADRWVAGFLEMFEEGCHKMGT 256

Query: 266 AIRDRIQERLM---GAQQQKYLENGKDSDSDLEEEYYYDDDDEEYYEDD 312
           AIRD IQ+RLM     + ++ L+NG     D ++E     DD E+ E+D
Sbjct: 257 AIRDGIQQRLMRQESEENRRLLQNGLTISKDNDDEQM--SDDNEFAEED 290

BLAST of CmoCh01G013500 vs. ExPASy Swiss-Prot
Match: Q9Y5K3 (Choline-phosphate cytidylyltransferase B OS=Homo sapiens OX=9606 GN=PCYT1B PE=1 SV=1)

HSP 1 Score: 260.8 bits (665), Expect = 2.2e-68
Identity = 130/232 (56.03%), Postives = 165/232 (71.12%), Query Frame = 0

Query: 25  PTDRPVRVYADGIYDLFHFGHARSLEQAKKSFPNTYLLVGCCNDETTHSLKGKTVMNEAE 84
           P DRPVRVYADGI+DLFH GHAR+L QAK  FPN+YLLVG C+D+ TH  KG TVMNEAE
Sbjct: 72  PADRPVRVYADGIFDLFHSGHARALMQAKTLFPNSYLLVGVCSDDLTHKFKGFTVMNEAE 131

Query: 85  RYESLRHCKWVDEVIPDAPWVISQEFIDKHKIDFVAHDSLPYADATGAGKDVYEFVKKIG 144
           RYE+LRHC++VDEVI DAPW ++ EF++KHKIDFVAHD +PY+ A     DVY+ +K+ G
Sbjct: 132 RYEALRHCRYVDEVIRDAPWTLTPEFLEKHKIDFVAHDDIPYSSA--GSDDVYKHIKEAG 191

Query: 145 KFKETKRTEGISTSDIIMRIVKDYNQYVLRNLDRGYSRKELGVSFQLNLDVFNYQEKRLR 204
            F  T+RTEGISTSDII RIV+DY+ Y  RNL RGY+ KEL VSF          EKR R
Sbjct: 192 MFVPTQRTEGISTSDIITRIVRDYDVYARRNLQRGYTAKELNVSF--------INEKRYR 251

Query: 205 VNMRLKKLQEKVKEHQEKVGEKIQTVAKTAGMHRNEWVENADRWVAGFLEMF 257
              ++ K++EKVK  +E+  E +  V + +     +W E +  ++  FLE+F
Sbjct: 252 FQNQVDKMKEKVKNVEERSKEFVNRVEEKSHDLIQKWEEKSREFIGNFLELF 293

BLAST of CmoCh01G013500 vs. ExPASy Swiss-Prot
Match: Q811Q9 (Choline-phosphate cytidylyltransferase B OS=Mus musculus OX=10090 GN=Pcyt1b PE=1 SV=2)

HSP 1 Score: 259.6 bits (662), Expect = 5.0e-68
Identity = 129/232 (55.60%), Postives = 165/232 (71.12%), Query Frame = 0

Query: 25  PTDRPVRVYADGIYDLFHFGHARSLEQAKKSFPNTYLLVGCCNDETTHSLKGKTVMNEAE 84
           P DRPVRVYADGI+DLFH GHAR+L QAK  FPN+YLLVG C+D+ TH  KG TVMNEAE
Sbjct: 72  PVDRPVRVYADGIFDLFHSGHARALMQAKTLFPNSYLLVGVCSDDLTHKFKGFTVMNEAE 131

Query: 85  RYESLRHCKWVDEVIPDAPWVISQEFIDKHKIDFVAHDSLPYADATGAGKDVYEFVKKIG 144
           RYE+LRHC++VDEVI DAPW ++ EF++KHKIDFVAHD +PY+ A     DVY+ +K+ G
Sbjct: 132 RYEALRHCRYVDEVIRDAPWTLTPEFLEKHKIDFVAHDDIPYSSA--GSDDVYKHIKEAG 191

Query: 145 KFKETKRTEGISTSDIIMRIVKDYNQYVLRNLDRGYSRKELGVSFQLNLDVFNYQEKRLR 204
            F  T+RTEGISTSDII RIV+DY+ Y  RNL RGY+ KEL VSF          EK+ R
Sbjct: 192 MFVPTQRTEGISTSDIITRIVRDYDVYARRNLQRGYTAKELNVSF--------INEKKYR 251

Query: 205 VNMRLKKLQEKVKEHQEKVGEKIQTVAKTAGMHRNEWVENADRWVAGFLEMF 257
              ++ K++EKVK  +E+  E +  V + +     +W E +  ++  FLE+F
Sbjct: 252 FQNQVDKMKEKVKNVEERSKEFVNRVEEKSHDLIQKWEEKSREFIGNFLELF 293

BLAST of CmoCh01G013500 vs. ExPASy Swiss-Prot
Match: Q9QZC4 (Choline-phosphate cytidylyltransferase B OS=Rattus norvegicus OX=10116 GN=Pcyt1b PE=1 SV=1)

HSP 1 Score: 259.6 bits (662), Expect = 5.0e-68
Identity = 129/232 (55.60%), Postives = 165/232 (71.12%), Query Frame = 0

Query: 25  PTDRPVRVYADGIYDLFHFGHARSLEQAKKSFPNTYLLVGCCNDETTHSLKGKTVMNEAE 84
           P DRPVRVYADGI+DLFH GHAR+L QAK  FPN+YLLVG C+D+ TH  KG TVMNEAE
Sbjct: 72  PVDRPVRVYADGIFDLFHSGHARALMQAKTLFPNSYLLVGVCSDDLTHKFKGFTVMNEAE 131

Query: 85  RYESLRHCKWVDEVIPDAPWVISQEFIDKHKIDFVAHDSLPYADATGAGKDVYEFVKKIG 144
           RYE+LRHC++VDEVI DAPW ++ EF++KHKIDFVAHD +PY+ A     DVY+ +K+ G
Sbjct: 132 RYEALRHCRYVDEVIRDAPWTLTPEFLEKHKIDFVAHDDIPYSSA--GSDDVYKHIKEAG 191

Query: 145 KFKETKRTEGISTSDIIMRIVKDYNQYVLRNLDRGYSRKELGVSFQLNLDVFNYQEKRLR 204
            F  T+RTEGISTSDII RIV+DY+ Y  RNL RGY+ KEL VSF          EK+ R
Sbjct: 192 MFVPTQRTEGISTSDIITRIVRDYDVYARRNLQRGYTAKELNVSF--------INEKKYR 251

Query: 205 VNMRLKKLQEKVKEHQEKVGEKIQTVAKTAGMHRNEWVENADRWVAGFLEMF 257
              ++ K++EKVK  +E+  E +  V + +     +W E +  ++  FLE+F
Sbjct: 252 FQNQVDKMKEKVKNVEERSKEFVNRVEEKSHDLIQKWEEKSREFIGNFLELF 293

BLAST of CmoCh01G013500 vs. ExPASy TrEMBL
Match: A0A6J1FJ47 (choline-phosphate cytidylyltransferase 2-like OS=Cucurbita moschata OX=3662 GN=LOC111445839 PE=3 SV=1)

HSP 1 Score: 623.2 bits (1606), Expect = 6.3e-175
Identity = 313/323 (96.90%), Postives = 314/323 (97.21%), Query Frame = 0

Query: 1   MAEVKPADSTTTINASPNHSNPDYPTDRPVRVYADGIYDLFHFGHARSLEQAKKSFPNTY 60
           MAEVKPADSTTTINASPNHSNPDYPTDRPVRVYADGIYDLFHFGHARSLEQAKKSFPNTY
Sbjct: 1   MAEVKPADSTTTINASPNHSNPDYPTDRPVRVYADGIYDLFHFGHARSLEQAKKSFPNTY 60

Query: 61  LLVGCCNDETTHSLKGKTVMNEAERYESLRHCKWVDEVIPDAPWVISQEFIDKHKIDFVA 120
           LLVGCCNDETTHSLKGKTVMNEAERYESLRHCKWVDEVIPDAPWVISQEFIDKHKIDFVA
Sbjct: 61  LLVGCCNDETTHSLKGKTVMNEAERYESLRHCKWVDEVIPDAPWVISQEFIDKHKIDFVA 120

Query: 121 HDSLPYADATGAGKDVYEFVKKIGKFKETKRTEGISTSDIIMRIVKDYNQYVLRNLDRGY 180
           HDSLPYADATGAGKDVYEFVKKIGKFKETKRTEGISTSDIIMRIVKDYNQYVLRNLDRGY
Sbjct: 121 HDSLPYADATGAGKDVYEFVKKIGKFKETKRTEGISTSDIIMRIVKDYNQYVLRNLDRGY 180

Query: 181 SRKELGVSFQLNLDVFNYQEKRLRVNMRLKKLQEKVKEHQEKVGEKIQTVAKTAGMHRNE 240
           SRKELGVSF         +EKRLRVNMRLKKLQEKVKEHQEKVGEKIQTVAKTAGMHRNE
Sbjct: 181 SRKELGVSF--------VKEKRLRVNMRLKKLQEKVKEHQEKVGEKIQTVAKTAGMHRNE 240

Query: 241 WVENADRWVAGFLEMFEEGCHKMGTAIRDRIQERLMGAQQQKYLENGKDSDSDLEEEYYY 300
           WVENADRWVAGFLEMFEEGCHKMGTAIRDRIQERLMGAQQQKYLENGKDSDSDLEEEYYY
Sbjct: 241 WVENADRWVAGFLEMFEEGCHKMGTAIRDRIQERLMGAQQQKYLENGKDSDSDLEEEYYY 300

Query: 301 DDDDEEYYEDDFYEEFEKDEKKR 324
           DDDDEEYYEDDFYEEFEKDEKKR
Sbjct: 301 DDDDEEYYEDDFYEEFEKDEKKR 315

BLAST of CmoCh01G013500 vs. ExPASy TrEMBL
Match: A0A6J1J168 (choline-phosphate cytidylyltransferase 2-like OS=Cucurbita maxima OX=3661 GN=LOC111480356 PE=3 SV=1)

HSP 1 Score: 614.8 bits (1584), Expect = 2.3e-172
Identity = 311/324 (95.99%), Postives = 313/324 (96.60%), Query Frame = 0

Query: 1   MAEVKPADSTTTINASPNHSNPDYPTDRPVRVYADGIYDLFHFGHARSLEQAKKSFPNTY 60
           MAEVKPADSTTTINASPNHSNPDYPTDRPVRVYADGIYDLFHFGHARSLEQAKKSFPNTY
Sbjct: 1   MAEVKPADSTTTINASPNHSNPDYPTDRPVRVYADGIYDLFHFGHARSLEQAKKSFPNTY 60

Query: 61  LLVGCCNDETTHSLKGKTVMNEAERYESLRHCKWVDEVIPDAPWVISQEFIDKHKIDFVA 120
           LLVGCCNDETTH+LKGKTVMNEAERYESLRHCKWVDEVIPDAPWVISQEFIDKHKIDFVA
Sbjct: 61  LLVGCCNDETTHNLKGKTVMNEAERYESLRHCKWVDEVIPDAPWVISQEFIDKHKIDFVA 120

Query: 121 HDSLPYADATGAGKDVYEFVKKIGKFKETKRTEGISTSDIIMRIVKDYNQYVLRNLDRGY 180
           HDSLPYADATGAGKDVYEFVKKIGKFKETKRTEGISTSDIIMRIVKDYNQYVLRNLDRGY
Sbjct: 121 HDSLPYADATGAGKDVYEFVKKIGKFKETKRTEGISTSDIIMRIVKDYNQYVLRNLDRGY 180

Query: 181 SRKELGVSFQLNLDVFNYQEKRLRVNMRLKKLQEKVKEHQEKVGEKIQTVAKTAGMHRNE 240
           SRKELGVSF         +EKRLRVNMRLKKLQEKVKEHQEKVGEKIQTVAKTAGMHRNE
Sbjct: 181 SRKELGVSF--------VKEKRLRVNMRLKKLQEKVKEHQEKVGEKIQTVAKTAGMHRNE 240

Query: 241 WVENADRWVAGFLEMFEEGCHKMGTAIRDRIQERLMGAQQQKYLENGKDSDSDLEEEYYY 300
           WVENADRWVAGFLEMFEEGCHKMGTAIRDRIQERLMGAQQQKYLENGKDSDSDLEEEYYY
Sbjct: 241 WVENADRWVAGFLEMFEEGCHKMGTAIRDRIQERLMGAQQQKYLENGKDSDSDLEEEYYY 300

Query: 301 DDDD-EEYYEDDFYEEFEKDEKKR 324
           DDDD EEYYED FYEEFEKDEKKR
Sbjct: 301 DDDDEEEYYEDGFYEEFEKDEKKR 316

BLAST of CmoCh01G013500 vs. ExPASy TrEMBL
Match: A0A1S3CM33 (choline-phosphate cytidylyltransferase 2-like OS=Cucumis melo OX=3656 GN=LOC103502481 PE=3 SV=1)

HSP 1 Score: 573.9 bits (1478), Expect = 4.4e-160
Identity = 296/336 (88.10%), Postives = 303/336 (90.18%), Query Frame = 0

Query: 1   MAEVKPADSTTTI---------NASPNHSNPDYPTDRPVRVYADGIYDLFHFGHARSLEQ 60
           MAEVKP DSTT I         N S NHSN D PTDRPVRVYADGIYDLFHFGHARSLEQ
Sbjct: 1   MAEVKPGDSTTNITTDTTTTAFNGSQNHSNSDQPTDRPVRVYADGIYDLFHFGHARSLEQ 60

Query: 61  AKKSFPNTYLLVGCCNDETTHSLKGKTVMNEAERYESLRHCKWVDEVIPDAPWVISQEFI 120
           AKKSFPNT+LLVGCCNDETTH  KGKTVM EAERYESLRHCKWVDEVIPDAPWVISQEFI
Sbjct: 61  AKKSFPNTFLLVGCCNDETTHKFKGKTVMTEAERYESLRHCKWVDEVIPDAPWVISQEFI 120

Query: 121 DKHKIDFVAHDSLPYADATGAGKDVYEFVKKIGKFKETKRTEGISTSDIIMRIVKDYNQY 180
           DKHKIDFVAHDSLPYADA+GAGKDVYEFVKKIGKFKETKRTEGISTSDIIMRIVKDYNQY
Sbjct: 121 DKHKIDFVAHDSLPYADASGAGKDVYEFVKKIGKFKETKRTEGISTSDIIMRIVKDYNQY 180

Query: 181 VLRNLDRGYSRKELGVSFQLNLDVFNYQEKRLRVNMRLKKLQEKVKEHQEKVGEKIQTVA 240
           VLRNLDRGYSRKELGVS+         +EKRLRVNMRLKKLQEKVKEHQEKVGEKIQTVA
Sbjct: 181 VLRNLDRGYSRKELGVSY--------VKEKRLRVNMRLKKLQEKVKEHQEKVGEKIQTVA 240

Query: 241 KTAGMHRNEWVENADRWVAGFLEMFEEGCHKMGTAIRDRIQERLMGAQQQKYLENGKDSD 300
           KTAGMHRNEWVENADRWVAGFLEMFEEGCHKMGTAIRDRIQERLMGAQQ KY+ NGKDSD
Sbjct: 241 KTAGMHRNEWVENADRWVAGFLEMFEEGCHKMGTAIRDRIQERLMGAQQLKYISNGKDSD 300

Query: 301 SDLEEEYYYDDDD-EEYY---EDDFYEEFEKDEKKR 324
           SDL+EEYYYDDDD EEYY   +DDFYEEFEKDEKKR
Sbjct: 301 SDLDEEYYYDDDDNEEYYDDDDDDFYEEFEKDEKKR 328

BLAST of CmoCh01G013500 vs. ExPASy TrEMBL
Match: A0A6J1FCK6 (choline-phosphate cytidylyltransferase 2-like OS=Cucurbita moschata OX=3662 GN=LOC111442868 PE=3 SV=1)

HSP 1 Score: 567.0 bits (1460), Expect = 5.4e-158
Identity = 295/340 (86.76%), Postives = 303/340 (89.12%), Query Frame = 0

Query: 1   MAEVKPADS------------TTTINASPNHSNPDYPTDRPVRVYADGIYDLFHFGHARS 60
           MAEV P +S            TTTIN S NHSN D PTDRPVRVYADGIYDLFHFGHARS
Sbjct: 1   MAEVNPGESTTTTTTITTTTTTTTINGSQNHSNSDQPTDRPVRVYADGIYDLFHFGHARS 60

Query: 61  LEQAKKSFPNTYLLVGCCNDETTHSLKGKTVMNEAERYESLRHCKWVDEVIPDAPWVISQ 120
           LEQAKKSFPNTYLLVGCCNDETTH  KGKTVM EAERYESLRHCKWVDEVIPDAPWVISQ
Sbjct: 61  LEQAKKSFPNTYLLVGCCNDETTHKFKGKTVMTEAERYESLRHCKWVDEVIPDAPWVISQ 120

Query: 121 EFIDKHKIDFVAHDSLPYADATGAGKDVYEFVKKIGKFKETKRTEGISTSDIIMRIVKDY 180
           EFIDKH ID+VAHDSLPYADA+GAGKDVYEFVKKIGKFKETKRT+GISTSDIIMRIVKDY
Sbjct: 121 EFIDKHNIDYVAHDSLPYADASGAGKDVYEFVKKIGKFKETKRTDGISTSDIIMRIVKDY 180

Query: 181 NQYVLRNLDRGYSRKELGVSFQLNLDVFNYQEKRLRVNMRLKKLQEKVKEHQEKVGEKIQ 240
           NQYVLRNLDRGYSRKELGVSF         +EKRLRVNMRLKKLQEKVKEHQEKVGEKIQ
Sbjct: 181 NQYVLRNLDRGYSRKELGVSF--------VKEKRLRVNMRLKKLQEKVKEHQEKVGEKIQ 240

Query: 241 TVAKTAGMHRNEWVENADRWVAGFLEMFEEGCHKMGTAIRDRIQERLMGAQQQKYLENGK 300
           TVAKTAGMHRNEWVENADRWVAGFLEMFEEGCHKMGTAIRDRIQERLMGAQQQKYL NGK
Sbjct: 241 TVAKTAGMHRNEWVENADRWVAGFLEMFEEGCHKMGTAIRDRIQERLMGAQQQKYLSNGK 300

Query: 301 DSD-SDLEEEYYYDDDD-EEYY---EDDFYEEFEKDEKKR 324
           DSD SDL+EEYYYDD+D EEYY   +DDFYEEFEKDEKKR
Sbjct: 301 DSDYSDLDEEYYYDDEDNEEYYDDDDDDFYEEFEKDEKKR 332

BLAST of CmoCh01G013500 vs. ExPASy TrEMBL
Match: A0A6J1IPQ3 (choline-phosphate cytidylyltransferase 2-like OS=Cucurbita maxima OX=3661 GN=LOC111477078 PE=3 SV=1)

HSP 1 Score: 566.6 bits (1459), Expect = 7.0e-158
Identity = 294/340 (86.47%), Postives = 303/340 (89.12%), Query Frame = 0

Query: 1   MAEVKPADS------------TTTINASPNHSNPDYPTDRPVRVYADGIYDLFHFGHARS 60
           MAEV P +S            TTT+N S NHSN D PTDRPVRVYADGIYDLFHFGHARS
Sbjct: 1   MAEVNPGESTTTTTTITTTTTTTTVNGSQNHSNSDQPTDRPVRVYADGIYDLFHFGHARS 60

Query: 61  LEQAKKSFPNTYLLVGCCNDETTHSLKGKTVMNEAERYESLRHCKWVDEVIPDAPWVISQ 120
           LEQAKKSFPNTYLLVGCCNDETTH  KGKTVM EAERYESLRHCKWVDEVIPDAPWVISQ
Sbjct: 61  LEQAKKSFPNTYLLVGCCNDETTHKFKGKTVMTEAERYESLRHCKWVDEVIPDAPWVISQ 120

Query: 121 EFIDKHKIDFVAHDSLPYADATGAGKDVYEFVKKIGKFKETKRTEGISTSDIIMRIVKDY 180
           EFIDKH ID+VAHDSLPYADA+GAGKDVYEFVKKIGKFKETKRT+GISTSDIIMRIVKDY
Sbjct: 121 EFIDKHNIDYVAHDSLPYADASGAGKDVYEFVKKIGKFKETKRTDGISTSDIIMRIVKDY 180

Query: 181 NQYVLRNLDRGYSRKELGVSFQLNLDVFNYQEKRLRVNMRLKKLQEKVKEHQEKVGEKIQ 240
           NQYVLRNLDRGYSRKELGVSF         +EKRLRVNMRLKKLQEKVKEHQEKVGEKIQ
Sbjct: 181 NQYVLRNLDRGYSRKELGVSF--------VKEKRLRVNMRLKKLQEKVKEHQEKVGEKIQ 240

Query: 241 TVAKTAGMHRNEWVENADRWVAGFLEMFEEGCHKMGTAIRDRIQERLMGAQQQKYLENGK 300
           TVAKTAGMHRNEWVENADRWVAGFLEMFEEGCHKMGTAIRDRIQERLMGAQQQKYL NGK
Sbjct: 241 TVAKTAGMHRNEWVENADRWVAGFLEMFEEGCHKMGTAIRDRIQERLMGAQQQKYLSNGK 300

Query: 301 DSD-SDLEEEYYYDDDD-EEYY---EDDFYEEFEKDEKKR 324
           DSD SDL+EEYYYDD+D EEYY   +DDFYEEFEKDEKKR
Sbjct: 301 DSDYSDLDEEYYYDDEDNEEYYDDDDDDFYEEFEKDEKKR 332

BLAST of CmoCh01G013500 vs. NCBI nr
Match: XP_022940119.1 (choline-phosphate cytidylyltransferase 2-like [Cucurbita moschata])

HSP 1 Score: 623.2 bits (1606), Expect = 1.3e-174
Identity = 313/323 (96.90%), Postives = 314/323 (97.21%), Query Frame = 0

Query: 1   MAEVKPADSTTTINASPNHSNPDYPTDRPVRVYADGIYDLFHFGHARSLEQAKKSFPNTY 60
           MAEVKPADSTTTINASPNHSNPDYPTDRPVRVYADGIYDLFHFGHARSLEQAKKSFPNTY
Sbjct: 1   MAEVKPADSTTTINASPNHSNPDYPTDRPVRVYADGIYDLFHFGHARSLEQAKKSFPNTY 60

Query: 61  LLVGCCNDETTHSLKGKTVMNEAERYESLRHCKWVDEVIPDAPWVISQEFIDKHKIDFVA 120
           LLVGCCNDETTHSLKGKTVMNEAERYESLRHCKWVDEVIPDAPWVISQEFIDKHKIDFVA
Sbjct: 61  LLVGCCNDETTHSLKGKTVMNEAERYESLRHCKWVDEVIPDAPWVISQEFIDKHKIDFVA 120

Query: 121 HDSLPYADATGAGKDVYEFVKKIGKFKETKRTEGISTSDIIMRIVKDYNQYVLRNLDRGY 180
           HDSLPYADATGAGKDVYEFVKKIGKFKETKRTEGISTSDIIMRIVKDYNQYVLRNLDRGY
Sbjct: 121 HDSLPYADATGAGKDVYEFVKKIGKFKETKRTEGISTSDIIMRIVKDYNQYVLRNLDRGY 180

Query: 181 SRKELGVSFQLNLDVFNYQEKRLRVNMRLKKLQEKVKEHQEKVGEKIQTVAKTAGMHRNE 240
           SRKELGVSF         +EKRLRVNMRLKKLQEKVKEHQEKVGEKIQTVAKTAGMHRNE
Sbjct: 181 SRKELGVSF--------VKEKRLRVNMRLKKLQEKVKEHQEKVGEKIQTVAKTAGMHRNE 240

Query: 241 WVENADRWVAGFLEMFEEGCHKMGTAIRDRIQERLMGAQQQKYLENGKDSDSDLEEEYYY 300
           WVENADRWVAGFLEMFEEGCHKMGTAIRDRIQERLMGAQQQKYLENGKDSDSDLEEEYYY
Sbjct: 241 WVENADRWVAGFLEMFEEGCHKMGTAIRDRIQERLMGAQQQKYLENGKDSDSDLEEEYYY 300

Query: 301 DDDDEEYYEDDFYEEFEKDEKKR 324
           DDDDEEYYEDDFYEEFEKDEKKR
Sbjct: 301 DDDDEEYYEDDFYEEFEKDEKKR 315

BLAST of CmoCh01G013500 vs. NCBI nr
Match: XP_022981108.1 (choline-phosphate cytidylyltransferase 2-like [Cucurbita maxima])

HSP 1 Score: 614.8 bits (1584), Expect = 4.7e-172
Identity = 311/324 (95.99%), Postives = 313/324 (96.60%), Query Frame = 0

Query: 1   MAEVKPADSTTTINASPNHSNPDYPTDRPVRVYADGIYDLFHFGHARSLEQAKKSFPNTY 60
           MAEVKPADSTTTINASPNHSNPDYPTDRPVRVYADGIYDLFHFGHARSLEQAKKSFPNTY
Sbjct: 1   MAEVKPADSTTTINASPNHSNPDYPTDRPVRVYADGIYDLFHFGHARSLEQAKKSFPNTY 60

Query: 61  LLVGCCNDETTHSLKGKTVMNEAERYESLRHCKWVDEVIPDAPWVISQEFIDKHKIDFVA 120
           LLVGCCNDETTH+LKGKTVMNEAERYESLRHCKWVDEVIPDAPWVISQEFIDKHKIDFVA
Sbjct: 61  LLVGCCNDETTHNLKGKTVMNEAERYESLRHCKWVDEVIPDAPWVISQEFIDKHKIDFVA 120

Query: 121 HDSLPYADATGAGKDVYEFVKKIGKFKETKRTEGISTSDIIMRIVKDYNQYVLRNLDRGY 180
           HDSLPYADATGAGKDVYEFVKKIGKFKETKRTEGISTSDIIMRIVKDYNQYVLRNLDRGY
Sbjct: 121 HDSLPYADATGAGKDVYEFVKKIGKFKETKRTEGISTSDIIMRIVKDYNQYVLRNLDRGY 180

Query: 181 SRKELGVSFQLNLDVFNYQEKRLRVNMRLKKLQEKVKEHQEKVGEKIQTVAKTAGMHRNE 240
           SRKELGVSF         +EKRLRVNMRLKKLQEKVKEHQEKVGEKIQTVAKTAGMHRNE
Sbjct: 181 SRKELGVSF--------VKEKRLRVNMRLKKLQEKVKEHQEKVGEKIQTVAKTAGMHRNE 240

Query: 241 WVENADRWVAGFLEMFEEGCHKMGTAIRDRIQERLMGAQQQKYLENGKDSDSDLEEEYYY 300
           WVENADRWVAGFLEMFEEGCHKMGTAIRDRIQERLMGAQQQKYLENGKDSDSDLEEEYYY
Sbjct: 241 WVENADRWVAGFLEMFEEGCHKMGTAIRDRIQERLMGAQQQKYLENGKDSDSDLEEEYYY 300

Query: 301 DDDD-EEYYEDDFYEEFEKDEKKR 324
           DDDD EEYYED FYEEFEKDEKKR
Sbjct: 301 DDDDEEEYYEDGFYEEFEKDEKKR 316

BLAST of CmoCh01G013500 vs. NCBI nr
Match: KAG6607965.1 (Choline-phosphate cytidylyltransferase 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 614.0 bits (1582), Expect = 7.9e-172
Identity = 311/323 (96.28%), Postives = 312/323 (96.59%), Query Frame = 0

Query: 1   MAEVKPADSTTTINASPNHSNPDYPTDRPVRVYADGIYDLFHFGHARSLEQAKKSFPNTY 60
           MAEVK ADSTTTINASPNHSNPDYPTDRPVRVYADGIYDLFHFGHARSLEQAKKSFPNTY
Sbjct: 1   MAEVKSADSTTTINASPNHSNPDYPTDRPVRVYADGIYDLFHFGHARSLEQAKKSFPNTY 60

Query: 61  LLVGCCNDETTHSLKGKTVMNEAERYESLRHCKWVDEVIPDAPWVISQEFIDKHKIDFVA 120
           LLVGCCNDETTHSLKGKTVMNEAERYESLRHCKWVDEVIPDAPWVISQEFIDKHKIDFVA
Sbjct: 61  LLVGCCNDETTHSLKGKTVMNEAERYESLRHCKWVDEVIPDAPWVISQEFIDKHKIDFVA 120

Query: 121 HDSLPYADATGAGKDVYEFVKKIGKFKETKRTEGISTSDIIMRIVKDYNQYVLRNLDRGY 180
           HDSLPYADATGAGKDVYEFVKKIGKFKETKRTEGISTSDIIMRIVKDYNQYVLRNLDRGY
Sbjct: 121 HDSLPYADATGAGKDVYEFVKKIGKFKETKRTEGISTSDIIMRIVKDYNQYVLRNLDRGY 180

Query: 181 SRKELGVSFQLNLDVFNYQEKRLRVNMRLKKLQEKVKEHQEKVGEKIQTVAKTAGMHRNE 240
           SRKELGVSF         +EKRLRVNMRLKKLQEKVKEHQEKVGEKIQTVAKTAGMHRNE
Sbjct: 181 SRKELGVSF--------VKEKRLRVNMRLKKLQEKVKEHQEKVGEKIQTVAKTAGMHRNE 240

Query: 241 WVENADRWVAGFLEMFEEGCHKMGTAIRDRIQERLMGAQQQKYLENGKDSDSDLEEEYYY 300
           WVENADRWVAGFLEMFEEGCHKMGTAIRDRIQERLMGAQQQKYLENGKDSDSDLEEEYYY
Sbjct: 241 WVENADRWVAGFLEMFEEGCHKMGTAIRDRIQERLMGAQQQKYLENGKDSDSDLEEEYYY 300

Query: 301 -DDDDEEYYEDDFYEEFEKDEKK 323
            DDDDEEYYEDDFYEEFEKDEKK
Sbjct: 301 DDDDDEEYYEDDFYEEFEKDEKK 315

BLAST of CmoCh01G013500 vs. NCBI nr
Match: XP_023523876.1 (choline-phosphate cytidylyltransferase 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 612.5 bits (1578), Expect = 2.3e-171
Identity = 310/324 (95.68%), Postives = 313/324 (96.60%), Query Frame = 0

Query: 1   MAEVKPADSTTTINASPNHSNPDYPTDRPVRVYADGIYDLFHFGHARSLEQAKKSFPNTY 60
           MAEVK ADSTTT+NASPN+SNPDYPTDRPVRVYADGIYDLFHFGHARSLEQAKKSFPNTY
Sbjct: 1   MAEVKSADSTTTLNASPNYSNPDYPTDRPVRVYADGIYDLFHFGHARSLEQAKKSFPNTY 60

Query: 61  LLVGCCNDETTHSLKGKTVMNEAERYESLRHCKWVDEVIPDAPWVISQEFIDKHKIDFVA 120
           LLVGCCNDETTHSLKGKTVMNEAERYESLRHCKWVDEVIPDAPWVISQEFIDKHKIDFVA
Sbjct: 61  LLVGCCNDETTHSLKGKTVMNEAERYESLRHCKWVDEVIPDAPWVISQEFIDKHKIDFVA 120

Query: 121 HDSLPYADATGAGKDVYEFVKKIGKFKETKRTEGISTSDIIMRIVKDYNQYVLRNLDRGY 180
           HDSLPYADATGAGKDVYEFVKKIGKFKETKRTEGISTSDIIMRIVKDYNQYVLRNLDRGY
Sbjct: 121 HDSLPYADATGAGKDVYEFVKKIGKFKETKRTEGISTSDIIMRIVKDYNQYVLRNLDRGY 180

Query: 181 SRKELGVSFQLNLDVFNYQEKRLRVNMRLKKLQEKVKEHQEKVGEKIQTVAKTAGMHRNE 240
           SRKELGVSF         +EKRLRVNMRLKKLQEKVKEHQEKVGEKIQTVAKTAGMHRNE
Sbjct: 181 SRKELGVSF--------VKEKRLRVNMRLKKLQEKVKEHQEKVGEKIQTVAKTAGMHRNE 240

Query: 241 WVENADRWVAGFLEMFEEGCHKMGTAIRDRIQERLMGAQQQKYLENGKDSDSDLEEEYYY 300
           WVENADRWVAGFLEMFEEGCHKMGTAIRDRIQERLMGAQQQKYLENGKDSDSDLEEEYYY
Sbjct: 241 WVENADRWVAGFLEMFEEGCHKMGTAIRDRIQERLMGAQQQKYLENGKDSDSDLEEEYYY 300

Query: 301 -DDDDEEYYEDDFYEEFEKDEKKR 324
            DDDDEEYYEDDFYEEFEKDEKKR
Sbjct: 301 DDDDDEEYYEDDFYEEFEKDEKKR 316

BLAST of CmoCh01G013500 vs. NCBI nr
Match: XP_004145998.1 (choline-phosphate cytidylyltransferase 2 isoform X1 [Cucumis sativus] >KGN55480.2 hypothetical protein Csa_012685 [Cucumis sativus])

HSP 1 Score: 575.5 bits (1482), Expect = 3.1e-160
Identity = 295/340 (86.76%), Postives = 303/340 (89.12%), Query Frame = 0

Query: 1   MAEVKPADS--------------TTTINASPNHSNPDYPTDRPVRVYADGIYDLFHFGHA 60
           MAEVKP DS              TT  N S NHSN D+PTDRPVRVYADGIYDLFHFGHA
Sbjct: 1   MAEVKPGDSTTNTTTTTITTTTATTAFNGSQNHSNSDHPTDRPVRVYADGIYDLFHFGHA 60

Query: 61  RSLEQAKKSFPNTYLLVGCCNDETTHSLKGKTVMNEAERYESLRHCKWVDEVIPDAPWVI 120
           RSLEQAKKSFPNT+LLVGCCNDETTH  KGKTVMNEAERYESLRHCKWVDEVIPDAPWVI
Sbjct: 61  RSLEQAKKSFPNTFLLVGCCNDETTHKFKGKTVMNEAERYESLRHCKWVDEVIPDAPWVI 120

Query: 121 SQEFIDKHKIDFVAHDSLPYADATGAGKDVYEFVKKIGKFKETKRTEGISTSDIIMRIVK 180
           SQEFIDKHKIDFVAHDSLPYADA+GAGKDVYEFVKKIGKFKETKRTEGISTSDIIMRIVK
Sbjct: 121 SQEFIDKHKIDFVAHDSLPYADASGAGKDVYEFVKKIGKFKETKRTEGISTSDIIMRIVK 180

Query: 181 DYNQYVLRNLDRGYSRKELGVSFQLNLDVFNYQEKRLRVNMRLKKLQEKVKEHQEKVGEK 240
           DYNQYVLRNLDRGYSRKELGVSF         +EKRLRVNMRLKKLQEKVKEHQEKVGEK
Sbjct: 181 DYNQYVLRNLDRGYSRKELGVSF--------VKEKRLRVNMRLKKLQEKVKEHQEKVGEK 240

Query: 241 IQTVAKTAGMHRNEWVENADRWVAGFLEMFEEGCHKMGTAIRDRIQERLMGAQQQKYLEN 300
           IQTVAKTAGMHRNEWVENADRWVAGFLEMFEEGCHKMGTAIRDRIQERL GAQQ KY+ N
Sbjct: 241 IQTVAKTAGMHRNEWVENADRWVAGFLEMFEEGCHKMGTAIRDRIQERLKGAQQLKYISN 300

Query: 301 GKDSDSDLEEEYYYDDDDEEYY---EDDFYEEFEKDEKKR 324
           GKDSDSDL+EEYYYDDD+EEYY   +DDFYEEFEKDEKKR
Sbjct: 301 GKDSDSDLDEEYYYDDDNEEYYDDDDDDFYEEFEKDEKKR 332

BLAST of CmoCh01G013500 vs. TAIR 10
Match: AT2G32260.1 (phosphorylcholine cytidylyltransferase )

HSP 1 Score: 439.1 bits (1128), Expect = 3.3e-123
Identity = 230/323 (71.21%), Postives = 266/323 (82.35%), Query Frame = 0

Query: 7   ADSTTTINASPNHSNPDYPTDRPVRVYADGIYDLFHFGHARSLEQAKKSFP-NTYLLVGC 66
           A  +T + +SP       PTDRPVRVYADGIYDLFHFGHARSLEQAK +FP NTYLLVGC
Sbjct: 19  ASGSTAVQSSP-------PTDRPVRVYADGIYDLFHFGHARSLEQAKLAFPNNTYLLVGC 78

Query: 67  CNDETTHSLKGKTVMNEAERYESLRHCKWVDEVIPDAPWVISQEFIDKHKIDFVAHDSLP 126
           CNDETTH  KG+TVM   ERYESLRHCKWVDEVIPDAPWV++QEF+DKH+ID+VAHDSLP
Sbjct: 79  CNDETTHKYKGRTVMTAEERYESLRHCKWVDEVIPDAPWVVNQEFLDKHQIDYVAHDSLP 138

Query: 127 YADATGAGKDVYEFVKKIGKFKETKRTEGISTSDIIMRIVKDYNQYVLRNLDRGYSRKEL 186
           YAD++GAGKDVYEFVKK+G+FKET+RTEGISTSDIIMRIVKDYNQYV+RNLDRGYSR++L
Sbjct: 139 YADSSGAGKDVYEFVKKVGRFKETQRTEGISTSDIIMRIVKDYNQYVMRNLDRGYSREDL 198

Query: 187 GVSFQLNLDVFNYQEKRLRVNMRLKKLQEKVKEHQEKVGEKIQTVAKTAGMHRNEWVENA 246
           GVSF         +EKRLRVNMRLKKLQE+VKE QE+VGEKIQTV     M RNEWVENA
Sbjct: 199 GVSF--------VKEKRLRVNMRLKKLQERVKEQQERVGEKIQTVK----MLRNEWVENA 258

Query: 247 DRWVAGFLEMFEEGCHKMGTAIRDRIQERLMGAQQQKYLENGKDSDSD--LEEEYY---- 306
           DRWVAGFLE+FEEGCHKMGTAI D IQERLM  +  + LENG+D D+D    EEY+    
Sbjct: 259 DRWVAGFLEIFEEGCHKMGTAIVDSIQERLMRQKSAERLENGQDDDTDDQFYEEYFDHDM 318

Query: 307 --YDDDDEEYYEDDFYEEFEKDE 321
              DD+DE++Y+++  +E E ++
Sbjct: 319 GSDDDEDEKFYDEEEVKEEETEK 322

BLAST of CmoCh01G013500 vs. TAIR 10
Match: AT4G15130.1 (phosphorylcholine cytidylyltransferase2 )

HSP 1 Score: 433.3 bits (1113), Expect = 1.8e-121
Identity = 220/289 (76.12%), Postives = 245/289 (84.78%), Query Frame = 0

Query: 26  TDRPVRVYADGIYDLFHFGHARSLEQAKKSFPNTYLLVGCCNDETTHSLKGKTVMNEAER 85
           +DRPVRVYADGI+DLFHFGHAR++EQAKKSFPNTYLLVGCCNDE T+  KGKTVM E+ER
Sbjct: 17  SDRPVRVYADGIFDLFHFGHARAIEQAKKSFPNTYLLVGCCNDEITNKFKGKTVMTESER 76

Query: 86  YESLRHCKWVDEVIPDAPWVISQEFIDKHKIDFVAHDSLPYADATGAGKDVYEFVKKIGK 145
           YESLRHCKWVDEVIPDAPWV++ EF+DKHKID+VAHD+LPYAD +GAG DVYEFVK IGK
Sbjct: 77  YESLRHCKWVDEVIPDAPWVLTTEFLDKHKIDYVAHDALPYADTSGAGNDVYEFVKSIGK 136

Query: 146 FKETKRTEGISTSDIIMRIVKDYNQYVLRNLDRGYSRKELGVSFQLNLDVFNYQEKRLRV 205
           FKETKRTEGISTSDIIMRIVKDYNQYVLRNLDRGYSR+ELGVSF         +EKRLRV
Sbjct: 137 FKETKRTEGISTSDIIMRIVKDYNQYVLRNLDRGYSREELGVSF---------EEKRLRV 196

Query: 206 NMRLKKLQEKVKEHQEKVGEKIQTVAKTAGMHRNEWVENADRWVAGFLEMFEEGCHKMGT 265
           NMRLKKLQEKVKE Q    EKIQTVAKTAGMH +EW+ENADRWVAGFLEMFEEGCHKMGT
Sbjct: 197 NMRLKKLQEKVKEQQ----EKIQTVAKTAGMHHDEWLENADRWVAGFLEMFEEGCHKMGT 256

Query: 266 AIRDRIQERLM---GAQQQKYLENGKDSDSDLEEEYYYDDDDEEYYEDD 312
           AIRD IQ+RLM     + ++ L+NG     D ++E     DD E+ E+D
Sbjct: 257 AIRDGIQQRLMRQESEENRRLLQNGLTISKDNDDEQM--SDDNEFAEED 290

BLAST of CmoCh01G013500 vs. TAIR 10
Match: AT2G38670.1 (phosphorylethanolamine cytidylyltransferase 1 )

HSP 1 Score: 114.4 bits (285), Expect = 1.8e-25
Identity = 62/166 (37.35%), Postives = 96/166 (57.83%), Query Frame = 0

Query: 28  RPVRVYADGIYDLFHFGHARSLEQAKKSFPNTYLLVGCCNDETTHSLKGKTVMNEAERYE 87
           +PVRVY DG +D+ H+GH  +L QA+       L+VG  +DE   + KG  V    ER  
Sbjct: 53  KPVRVYMDGCFDMMHYGHCNALRQARAL--GDQLVVGVVSDEEIIANKGPPVTPLHERMT 112

Query: 88  SLRHCKWVDEVIPDAPWVISQEFI----DKHKIDFVAHDSLPYADATGAGKDVYEFVKKI 147
            ++  KWVDEVI DAP+ I+++F+    D+++ID++ H   P       G D Y   KK 
Sbjct: 113 MVKAVKWVDEVISDAPYAITEDFMKKLFDEYQIDYIIHGDDPC--VLPDGTDAYALAKKA 172

Query: 148 GKFKETKRTEGISTSDIIMRIVKDYNQYVLRNLDRGYSRKELGVSF 190
           G++K+ KRTEG+S++DI+ R++    +   R++   +SR  L   F
Sbjct: 173 GRYKQIKRTEGVSSTDIVGRMLLCVRE---RSISDTHSRSSLQRQF 211

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9ZV564.6e-12271.21Choline-phosphate cytidylyltransferase 1 OS=Arabidopsis thaliana OX=3702 GN=CCT1... [more]
F4JJE02.5e-12076.12Choline-phosphate cytidylyltransferase 2 OS=Arabidopsis thaliana OX=3702 GN=CCT2... [more]
Q9Y5K32.2e-6856.03Choline-phosphate cytidylyltransferase B OS=Homo sapiens OX=9606 GN=PCYT1B PE=1 ... [more]
Q811Q95.0e-6855.60Choline-phosphate cytidylyltransferase B OS=Mus musculus OX=10090 GN=Pcyt1b PE=1... [more]
Q9QZC45.0e-6855.60Choline-phosphate cytidylyltransferase B OS=Rattus norvegicus OX=10116 GN=Pcyt1b... [more]
Match NameE-valueIdentityDescription
A0A6J1FJ476.3e-17596.90choline-phosphate cytidylyltransferase 2-like OS=Cucurbita moschata OX=3662 GN=L... [more]
A0A6J1J1682.3e-17295.99choline-phosphate cytidylyltransferase 2-like OS=Cucurbita maxima OX=3661 GN=LOC... [more]
A0A1S3CM334.4e-16088.10choline-phosphate cytidylyltransferase 2-like OS=Cucumis melo OX=3656 GN=LOC1035... [more]
A0A6J1FCK65.4e-15886.76choline-phosphate cytidylyltransferase 2-like OS=Cucurbita moschata OX=3662 GN=L... [more]
A0A6J1IPQ37.0e-15886.47choline-phosphate cytidylyltransferase 2-like OS=Cucurbita maxima OX=3661 GN=LOC... [more]
Match NameE-valueIdentityDescription
XP_022940119.11.3e-17496.90choline-phosphate cytidylyltransferase 2-like [Cucurbita moschata][more]
XP_022981108.14.7e-17295.99choline-phosphate cytidylyltransferase 2-like [Cucurbita maxima][more]
KAG6607965.17.9e-17296.28Choline-phosphate cytidylyltransferase 1, partial [Cucurbita argyrosperma subsp.... [more]
XP_023523876.12.3e-17195.68choline-phosphate cytidylyltransferase 1-like [Cucurbita pepo subsp. pepo][more]
XP_004145998.13.1e-16086.76choline-phosphate cytidylyltransferase 2 isoform X1 [Cucumis sativus] >KGN55480.... [more]
Match NameE-valueIdentityDescription
AT2G32260.13.3e-12371.21phosphorylcholine cytidylyltransferase [more]
AT4G15130.11.8e-12176.12phosphorylcholine cytidylyltransferase2 [more]
AT2G38670.11.8e-2537.35phosphorylethanolamine cytidylyltransferase 1 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 202..229
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 7..23
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..26
NoneNo IPR availablePANTHERPTHR10739:SF52CHOLINE-PHOSPHATE CYTIDYLYLTRANSFERASE 2-LIKE ISOFORM X1coord: 22..310
NoneNo IPR availableSUPERFAMILY52374Nucleotidylyl transferasecoord: 31..163
IPR004821Cytidyltransferase-like domainTIGRFAMTIGR00125TIGR00125coord: 31..98
e-value: 6.2E-20
score: 69.1
IPR004821Cytidyltransferase-like domainPFAMPF01467CTP_transf_likecoord: 33..162
e-value: 4.3E-32
score: 111.3
IPR014729Rossmann-like alpha/beta/alpha sandwich foldGENE3D3.40.50.620HUPscoord: 2..258
e-value: 1.3E-83
score: 281.9
IPR045049Choline-phosphate cytidylyltransferase Pcy1-likePANTHERPTHR10739CYTIDYLYLTRANSFERASEcoord: 22..310
IPR041723CTP:phosphocholine cytidylyltransferase domainCDDcd02174CCTcoord: 28..179
e-value: 2.04034E-92
score: 270.593

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh01G013500.1CmoCh01G013500.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006657 CDP-choline pathway
biological_process GO:0009058 biosynthetic process
molecular_function GO:0004105 choline-phosphate cytidylyltransferase activity
molecular_function GO:0031210 phosphatidylcholine binding
molecular_function GO:0003824 catalytic activity