CmoCh01G010040 (gene) Cucurbita moschata (Rifu) v1

Overview


Name	CmoCh01G010040
Type	gene
Organism	Cucurbita moschata (Cucurbita moschata (Rifu) v1)
Description	rRNA N-glycosidase
Location	Cmo_Chr01: 7159573 .. 7160490 (+)
RNA-Seq Expression	CmoCh01G010040
Synteny	CmoCh01G010040

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAGTGCATTGGATCAACCCAAGACTTGATACAAGCTCCCTTTCCCTTAAACTGTGGTGCTCATGCTGCCATTCGTGCTCCTCGTCCTTCCGCCAATGCTAATCTCAGCTTCTCTCTGTTGGGTGCCACTGCCCAAACCTACAAACAATTCATTAATGATCTTCGTAAGGCACTTACGGTAAACTCCCACAAAGTGTACGACATTCCTGTGCTGCCAGCCACAGCCAAAGGGTTAGCACGCTTCATATTGGTCAATCTCACCAATTACAAAGGTGAATCCATCACTGTTGCAATCGACACAGTGGATGTCTATATTGTCGCATACCAAGCAGGAAACAAGGCTTATTTTCTAAAAGATGTTTCCAAGGAAGCCCATGATGTGCTATTTAAAGGTAGCAAACATGAAACGCTGTCGTATAAAGGGAATTATGATGATCTTGAGAGGCATGCTGGTAAGATTTCAAGAGAGAATATTGAGCTTGGATTCTCTGAATTAAGCAGCTCCATTGGCAACATGTTCCACTATAAGGCTGGCTATTCTGTGCCTAGATCGTTTATTGTGATGATTCAAATGCTTTCAGAAGCTTCGAGGTTTAAGTATATTGAGACGAGAAATTGTGAGAAGGTTGAATCAAAGTTTAAGCCAGATCCTGGATACTTGAGTTTAGAGAACCGTTGGTCTAATTTATCTGAACAGGTACAGAAGGCGCAAAAGCATGGAGGTAAATTTGAGCATCCAGTTCAAGTTCGAAGTGTTAGCAATAAGCCAGTTCTGGTGAGTGATGTTAATTCCCCTGTTGTTAAAGGAATAGCGCTGTTACTTTCCAACAAGCCAGGGGTTAATGGAGTATCCAAATTGATGGAAGAGTTTATCCATTGGGAAGCTGCTCGAGGTCATTTTGGAATTGATTTCTGA

mRNA sequence

ATGGAGTGCATTGGATCAACCCAAGACTTGATACAAGCTCCCTTTCCCTTAAACTGTGGTGCTCATGCTGCCATTCGTGCTCCTCGTCCTTCCGCCAATGCTAATCTCAGCTTCTCTCTGTTGGGTGCCACTGCCCAAACCTACAAACAATTCATTAATGATCTTCGTAAGGCACTTACGGTAAACTCCCACAAAGTGTACGACATTCCTGTGCTGCCAGCCACAGCCAAAGGGTTAGCACGCTTCATATTGGTCAATCTCACCAATTACAAAGGTGAATCCATCACTGTTGCAATCGACACAGTGGATGTCTATATTGTCGCATACCAAGCAGGAAACAAGGCTTATTTTCTAAAAGATGTTTCCAAGGAAGCCCATGATGTGCTATTTAAAGGTAGCAAACATGAAACGCTGTCGTATAAAGGGAATTATGATGATCTTGAGAGGCATGCTGGTAAGATTTCAAGAGAGAATATTGAGCTTGGATTCTCTGAATTAAGCAGCTCCATTGGCAACATGTTCCACTATAAGGCTGGCTATTCTGTGCCTAGATCGTTTATTGTGATGATTCAAATGCTTTCAGAAGCTTCGAGGTTTAAGTATATTGAGACGAGAAATTGTGAGAAGGTTGAATCAAAGTTTAAGCCAGATCCTGGATACTTGAGTTTAGAGAACCGTTGGTCTAATTTATCTGAACAGGTACAGAAGGCGCAAAAGCATGGAGGTAAATTTGAGCATCCAGTTCAAGTTCGAAGTGTTAGCAATAAGCCAGTTCTGGTGAGTGATGTTAATTCCCCTGTTGTTAAAGGAATAGCGCTGTTACTTTCCAACAAGCCAGGGGTTAATGGAGTATCCAAATTGATGGAAGAGTTTATCCATTGGGAAGCTGCTCGAGGTCATTTTGGAATTGATTTCTGA

Coding sequence (CDS)

ATGGAGTGCATTGGATCAACCCAAGACTTGATACAAGCTCCCTTTCCCTTAAACTGTGGTGCTCATGCTGCCATTCGTGCTCCTCGTCCTTCCGCCAATGCTAATCTCAGCTTCTCTCTGTTGGGTGCCACTGCCCAAACCTACAAACAATTCATTAATGATCTTCGTAAGGCACTTACGGTAAACTCCCACAAAGTGTACGACATTCCTGTGCTGCCAGCCACAGCCAAAGGGTTAGCACGCTTCATATTGGTCAATCTCACCAATTACAAAGGTGAATCCATCACTGTTGCAATCGACACAGTGGATGTCTATATTGTCGCATACCAAGCAGGAAACAAGGCTTATTTTCTAAAAGATGTTTCCAAGGAAGCCCATGATGTGCTATTTAAAGGTAGCAAACATGAAACGCTGTCGTATAAAGGGAATTATGATGATCTTGAGAGGCATGCTGGTAAGATTTCAAGAGAGAATATTGAGCTTGGATTCTCTGAATTAAGCAGCTCCATTGGCAACATGTTCCACTATAAGGCTGGCTATTCTGTGCCTAGATCGTTTATTGTGATGATTCAAATGCTTTCAGAAGCTTCGAGGTTTAAGTATATTGAGACGAGAAATTGTGAGAAGGTTGAATCAAAGTTTAAGCCAGATCCTGGATACTTGAGTTTAGAGAACCGTTGGTCTAATTTATCTGAACAGGTACAGAAGGCGCAAAAGCATGGAGGTAAATTTGAGCATCCAGTTCAAGTTCGAAGTGTTAGCAATAAGCCAGTTCTGGTGAGTGATGTTAATTCCCCTGTTGTTAAAGGAATAGCGCTGTTACTTTCCAACAAGCCAGGGGTTAATGGAGTATCCAAATTGATGGAAGAGTTTATCCATTGGGAAGCTGCTCGAGGTCATTTTGGAATTGATTTCTGA

Protein sequence

MECIGSTQDLIQAPFPLNCGAHAAIRAPRPSANANLSFSLLGATAQTYKQFINDLRKALTVNSHKVYDIPVLPATAKGLARFILVNLTNYKGESITVAIDTVDVYIVAYQAGNKAYFLKDVSKEAHDVLFKGSKHETLSYKGNYDDLERHAGKISRENIELGFSELSSSIGNMFHYKAGYSVPRSFIVMIQMLSEASRFKYIETRNCEKVESKFKPDPGYLSLENRWSNLSEQVQKAQKHGGKFEHPVQVRSVSNKPVLVSDVNSPVVKGIALLLSNKPGVNGVSKLMEEFIHWEAARGHFGIDF

Homology

[Close]

BLAST of CmoCh01G010040 vs. ExPASy Swiss-Prot
Match: P98184 (Ribosome-inactivating protein bryodin II OS=Bryonia dioica OX=3652 PE=1 SV=1)

HSP 1 Score: 308.1 bits (788), Expect = 1.1e-82
Identity = 161/259 (62.16%), Postives = 196/259 (75.68%), Query Frame = 0


Query: 17  LNCGAHAAIRAPRPSANANLSFSLLGATAQTYKQFINDLRKALTVNSHKVYDIPVLPATA 76
           L  GAH        + + +++FSL+GAT  TYK FI +LR  LTV + +VYDIPVL   A
Sbjct: 12  LYVGAHV-------TEDVDINFSLIGATGATYKTFIRNLRTKLTVGTPRVYDIPVLRNAA 71

Query: 77  KGLARFILVNLTNYKGESITVAIDTVDVYIVAYQAGNKAYFLKDVSKEAHDVLFKGSKHE 136
            GLARF LV LTNY GES+TVA+D V+VY+VAY+AGN AYFL D S EA++VLF G  H 
Sbjct: 72  AGLARFQLVTLTNYNGESVTVALDVVNVYVVAYRAGNTAYFLADASTEANNVLFAGINHV 131

Query: 137 TLSYKGNYDDLERHAGKISRENIELGFSELSSSIGNMFHYKAGYSVPRSFIVMIQMLSEA 196
            L Y GNYD LE  AG+ISRENIELGFSE+SS+IGNMF +  G SVPR+FIV+IQ +SEA
Sbjct: 132 RLPYGGNYDGLETAAGRISRENIELGFSEISSAIGNMFRHNPGTSVPRAFIVIIQTVSEA 191

Query: 197 SRFKYIETRNCEKVESKFKPDPGYLSLENRWSNLSEQVQKAQKHGGKFEHPVQVRSVSNK 256
           +RFKYIE R  E V +KFKPDP +LSL+N W +LSEQ+Q AQ  GG+F  PV++R+VSN 
Sbjct: 192 ARFKYIEQRVSENVGTKFKPDPAFLSLQNAWGSLSEQIQIAQTRGGEFARPVELRTVSNT 251

Query: 257 PVLVSDVNSPVVKGIALLL 276
           P  V++VNSPVVKGIALLL
Sbjct: 252 PTFVTNVNSPVVKGIALLL 263

[Close]

BLAST of CmoCh01G010040 vs. ExPASy Swiss-Prot
Match: P24817 (Ribosome-inactivating protein beta-momorcharin OS=Momordica charantia OX=3673 GN=MAP30 PE=1 SV=2)

HSP 1 Score: 213.4 bits (542), Expect = 3.6e-54
Identity = 120/250 (48.00%), Postives = 172/250 (68.80%), Query Frame = 0


Query: 30  PSANANLSFSLLGATAQTYKQFINDLRKALTVNSHKVYDIPVLPATAKGLARFILVNLTN 89
           P+A  +++F L  ATA+TY +FI D R  L   SHKVYDIP+L +T     RFIL+NLT+
Sbjct: 19  PTAKGDVNFDLSTATAKTYTKFIEDFRATLPF-SHKVYDIPLLYSTISDSRRFILLNLTS 78

Query: 90  YKGESITVAIDTVDVYIVAYQAGNKAYFLKDVSKEAHDVLFKGSKHETLSYKGNYDDLER 149
           Y  E+I+VAID  +VY+VAY+  + +YF K+   EA+++LFKG++  TL Y GNY++L+ 
Sbjct: 79  YAYETISVAIDVTNVYVVAYRTRDVSYFFKESPPEAYNILFKGTRKITLPYTGNYENLQT 138

Query: 150 HAGKISRENIELGFSELSSSIGNMFHYKAGYSVPRSFIVMIQMLSEASRFKYIETRNCEK 209
            A KI RENI+LG   LSS+I  +F+Y A  S P + +V+IQ  +EA+RFKYIE    + 
Sbjct: 139 AAHKI-RENIDLGLPALSSAITTLFYYNA-QSAPSALLVLIQTTAEAARFKYIERHVAKY 198

Query: 210 VESKFKPDPGYLSLENRWSNLSEQVQKAQKHGGKFEHPVQVRSVSNKPVLVSDVNSPVVK 269
           V + FKP+   +SLEN+WS LS+Q+  AQ  GGKF +PV +   + +   V++V+S VVK
Sbjct: 199 VATNFKPNLAIISLENQWSALSKQIFLAQNQGGKFRNPVDLIKPTGERFQVTNVDSDVVK 258

Query: 270 G-IALLLSNK 279
           G I LLL+++
Sbjct: 259 GNIKLLLNSR 265

[Close]

BLAST of CmoCh01G010040 vs. ExPASy Swiss-Prot
Match: P29339 (Ribosome-inactivating protein momordin II OS=Momordica balsamina OX=3672 PE=3 SV=1)

HSP 1 Score: 211.5 bits (537), Expect = 1.4e-53
Identity = 119/250 (47.60%), Postives = 172/250 (68.80%), Query Frame = 0


Query: 30  PSANANLSFSLLGATAQTYKQFINDLRKALTVNSHKVYDIPVLPATAKGLARFILVNLTN 89
           P+A  +++F L  ATA+TY +FI D R  L   SHKVYDIP+L +T     RFIL++LT+
Sbjct: 19  PTAKGDVNFDLSTATAKTYTKFIEDFRATLPF-SHKVYDIPLLYSTISDSRRFILLDLTS 78

Query: 90  YKGESITVAIDTVDVYIVAYQAGNKAYFLKDVSKEAHDVLFKGSKHETLSYKGNYDDLER 149
           Y  E+I+VAID  +VY+VAY+  + +YF K+   EA+++LFKG++  TL Y GNY++L+ 
Sbjct: 79  YAYETISVAIDVTNVYVVAYRTRDVSYFFKESPPEAYNILFKGTRKITLPYTGNYENLQT 138

Query: 150 HAGKISRENIELGFSELSSSIGNMFHYKAGYSVPRSFIVMIQMLSEASRFKYIETRNCEK 209
            A KI RENI+LG   LSS+I  +F+Y A  S P + +V+IQ  +EA+RFKYIE    + 
Sbjct: 139 AAHKI-RENIDLGLPALSSAITTLFYYNA-QSAPSALLVLIQTTAEAARFKYIERHVAKY 198

Query: 210 VESKFKPDPGYLSLENRWSNLSEQVQKAQKHGGKFEHPVQVRSVSNKPVLVSDVNSPVVK 269
           V + FKP+   +SLEN+WS LS+Q+  AQ  GGKF +PV +   + +   V++V+S VVK
Sbjct: 199 VATNFKPNLAIISLENQWSALSKQIFLAQNQGGKFRNPVDLIKPTGERFQVTNVDSDVVK 258

Query: 270 G-IALLLSNK 279
           G I LLL+++
Sbjct: 259 GNIKLLLNSR 265

[Close]

BLAST of CmoCh01G010040 vs. ExPASy Swiss-Prot
Match: P84531 (Ribosome-inactivating protein cucurmosin OS=Cucurbita moschata OX=3662 PE=1 SV=2)

HSP 1 Score: 199.9 bits (507), Expect = 4.1e-50
Identity = 115/243 (47.33%), Postives = 158/243 (65.02%), Query Frame = 0


Query: 35  NLSFSLLGATAQTYKQFINDLRKALTVNSHKVYDIPVLPATAKGLARFILVNLTNYKGES 94
           N+ F L  AT+ +YK FI +LR+AL  +  KVYDIPVL +T     RFIL++L NY G+S
Sbjct: 1   NVRFDLSSATSSSYKTFIKNLREALPKDG-KVYDIPVLLSTVMDSRRFILIDLVNYDGQS 60

Query: 95  ITVAIDTVDVYIVAYQAGNKAYFLKDVSKEAHDVLFKGSKHETLSYKGNYDDLERHAGKI 154
           IT AID ++VYIVAY  G  +YF + V  +A  +LFKG++  TL Y GNY++L+  A K+
Sbjct: 61  ITAAIDVLNVYIVAYSTGTVSYFFQQVPAQAPKLLFKGTQQRTLPYTGNYENLQTAAKKL 120

Query: 155 SRENIELGFSELSSSIGNMFHYKAGYSVPRSFIVMIQMLSEASRFKYIETRNCEKVESKF 214
            RENIELG   L S+I  +FHY A  +   + +V+IQ  SEA+RF+YIE +    V +KF
Sbjct: 121 -RENIELGLPALDSAITTLFHYNA-EAAASALLVLIQTTSEAARFRYIELQIANNVGTKF 180

Query: 215 KPDPGYLSLENRWSNLSEQVQKAQKHGGKFEHPVQVRSVSNKPVLVSDVNSPVV-KGIAL 274
           KP    +SLEN WS LS+Q+Q A+   G+FE PV +       V +++V S VV + I L
Sbjct: 181 KPSQTIISLENNWSALSKQIQIAKNKNGQFETPVILIDPQGNRVQITNVTSNVVTQNIQL 240

Query: 275 LLS 277
           LL+
Sbjct: 241 LLN 240

[Close]

BLAST of CmoCh01G010040 vs. ExPASy Swiss-Prot
Match: P09989 (Ribosome-inactivating protein alpha-trichosanthin OS=Trichosanthes kirilowii OX=3677 PE=1 SV=2)

HSP 1 Score: 188.7 bits (478), Expect = 9.5e-47
Identity = 111/250 (44.40%), Postives = 165/250 (66.00%), Query Frame = 0


Query: 30  PSANANLSFSLLGATAQTYKQFINDLRKALTVNSHKVYDIPVLPATAKGLARFILVNLTN 89
           P+   ++SF L GAT+ +Y  FI++LRKAL  N  K+YDIP+L ++  G  R+ L++LTN
Sbjct: 19  PAVEGDVSFRLSGATSSSYGVFISNLRKALP-NERKLYDIPLLRSSLPGSQRYALIHLTN 78

Query: 90  YKGESITVAIDTVDVYIVAYQAGNKAYFLKDVS-KEAHDVLFKGSKHE-TLSYKGNYDDL 149
           Y  E+I+VAID  +VYI+ Y+AG+ +YF  + S  EA   +FK +  + TL Y GNY+ L
Sbjct: 79  YADETISVAIDVTNVYIMGYRAGDTSYFFNEASATEAAKYVFKDAMRKVTLPYSGNYERL 138

Query: 150 ERHAGKISRENIELGFSELSSSIGNMFHYKAGYSVPRSFIVMIQMLSEASRFKYIETRNC 209
           +  AGKI RENI LG   L S+I  +F+Y A  S   + +V+IQ  SEA+R+K+IE +  
Sbjct: 139 QTAAGKI-RENIPLGLPALDSAITTLFYYNAN-SAASALMVLIQSTSEAARYKFIEQQIG 198

Query: 210 EKVESKFKPDPGYLSLENRWSNLSEQVQKAQKHGGKFEHPVQVRSVSNKPVLVSDVNSPV 269
           ++V+  F P    +SLEN WS LS+Q+Q A  + G+FE PV + +  N+ V +++V++ V
Sbjct: 199 KRVDKTFLPSLAIISLENSWSALSKQIQIASTNNGQFESPVVLINAQNQRVTITNVDAGV 258

Query: 270 V-KGIALLLS 277
           V   IALLL+
Sbjct: 259 VTSNIALLLN 265

[Close]

BLAST of CmoCh01G010040 vs. ExPASy TrEMBL
Match: A0A6J1EKL0 (rRNA N-glycosidase OS=Cucurbita moschata OX=3662 GN=LOC111433434 PE=3 SV=1)

HSP 1 Score: 609.0 bits (1569), Expect = 1.1e-170
Identity = 305/305 (100.00%), Postives = 305/305 (100.00%), Query Frame = 0


Query: 1   MECIGSTQDLIQAPFPLNCGAHAAIRAPRPSANANLSFSLLGATAQTYKQFINDLRKALT 60
           MECIGSTQDLIQAPFPLNCGAHAAIRAPRPSANANLSFSLLGATAQTYKQFINDLRKALT
Sbjct: 1   MECIGSTQDLIQAPFPLNCGAHAAIRAPRPSANANLSFSLLGATAQTYKQFINDLRKALT 60

Query: 61  VNSHKVYDIPVLPATAKGLARFILVNLTNYKGESITVAIDTVDVYIVAYQAGNKAYFLKD 120
           VNSHKVYDIPVLPATAKGLARFILVNLTNYKGESITVAIDTVDVYIVAYQAGNKAYFLKD
Sbjct: 61  VNSHKVYDIPVLPATAKGLARFILVNLTNYKGESITVAIDTVDVYIVAYQAGNKAYFLKD 120

Query: 121 VSKEAHDVLFKGSKHETLSYKGNYDDLERHAGKISRENIELGFSELSSSIGNMFHYKAGY 180
           VSKEAHDVLFKGSKHETLSYKGNYDDLERHAGKISRENIELGFSELSSSIGNMFHYKAGY
Sbjct: 121 VSKEAHDVLFKGSKHETLSYKGNYDDLERHAGKISRENIELGFSELSSSIGNMFHYKAGY 180

Query: 181 SVPRSFIVMIQMLSEASRFKYIETRNCEKVESKFKPDPGYLSLENRWSNLSEQVQKAQKH 240
           SVPRSFIVMIQMLSEASRFKYIETRNCEKVESKFKPDPGYLSLENRWSNLSEQVQKAQKH
Sbjct: 181 SVPRSFIVMIQMLSEASRFKYIETRNCEKVESKFKPDPGYLSLENRWSNLSEQVQKAQKH 240

Query: 241 GGKFEHPVQVRSVSNKPVLVSDVNSPVVKGIALLLSNKPGVNGVSKLMEEFIHWEAARGH 300
           GGKFEHPVQVRSVSNKPVLVSDVNSPVVKGIALLLSNKPGVNGVSKLMEEFIHWEAARGH
Sbjct: 241 GGKFEHPVQVRSVSNKPVLVSDVNSPVVKGIALLLSNKPGVNGVSKLMEEFIHWEAARGH 300

Query: 301 FGIDF 306
           FGIDF
Sbjct: 301 FGIDF 305

[Close]

BLAST of CmoCh01G010040 vs. ExPASy TrEMBL
Match: A0A6J1IUB2 (rRNA N-glycosidase OS=Cucurbita maxima OX=3661 GN=LOC111480641 PE=3 SV=1)

HSP 1 Score: 589.3 bits (1518), Expect = 8.9e-165
Identity = 294/305 (96.39%), Postives = 299/305 (98.03%), Query Frame = 0


Query: 1   MECIGSTQDLIQAPFPLNCGAHAAIRAPRPSANANLSFSLLGATAQTYKQFINDLRKALT 60
           MECIGST+DLIQAPFPLNCGAH AIRAPRPSANANLSFSLLGATAQTYKQFI+DLRKALT
Sbjct: 1   MECIGSTKDLIQAPFPLNCGAHVAIRAPRPSANANLSFSLLGATAQTYKQFISDLRKALT 60

Query: 61  VNSHKVYDIPVLPATAKGLARFILVNLTNYKGESITVAIDTVDVYIVAYQAGNKAYFLKD 120
           VNSHKVYDIPVLPATAKGLARFILVNLTNYKGESITVAIDTVDVYIVAYQAGNKAYFLKD
Sbjct: 61  VNSHKVYDIPVLPATAKGLARFILVNLTNYKGESITVAIDTVDVYIVAYQAGNKAYFLKD 120

Query: 121 VSKEAHDVLFKGSKHETLSYKGNYDDLERHAGKISRENIELGFSELSSSIGNMFHYKAGY 180
           VSKEAHDVLFKGSKHETLSYKGNYDDLERHAGKISRENI+LGFSELSSSIGNMFHY AGY
Sbjct: 121 VSKEAHDVLFKGSKHETLSYKGNYDDLERHAGKISRENIDLGFSELSSSIGNMFHYNAGY 180

Query: 181 SVPRSFIVMIQMLSEASRFKYIETRNCEKVESKFKPDPGYLSLENRWSNLSEQVQKAQKH 240
           SVP+SFIVMIQMLSEASRFKYIE RNCE VESKFKPDPGYLSLENRWSNLSEQVQKAQKH
Sbjct: 181 SVPKSFIVMIQMLSEASRFKYIEARNCENVESKFKPDPGYLSLENRWSNLSEQVQKAQKH 240

Query: 241 GGKFEHPVQVRSVSNKPVLVSDVNSPVVKGIALLLSNKPGVNGVSKLMEEFIHWEAARGH 300
           GGKFEHPVQVRSVSNKPVLVSDVNSPVVKG+ALLL NKPGVNGVSKLMEEFI WEAARGH
Sbjct: 241 GGKFEHPVQVRSVSNKPVLVSDVNSPVVKGMALLLCNKPGVNGVSKLMEEFIKWEAARGH 300

Query: 301 FGIDF 306
           FGIDF
Sbjct: 301 FGIDF 305

[Close]

BLAST of CmoCh01G010040 vs. ExPASy TrEMBL
Match: A0A6J1E093 (rRNA N-glycosidase OS=Momordica charantia OX=3673 GN=LOC111024726 PE=3 SV=1)

HSP 1 Score: 388.3 bits (996), Expect = 3.0e-104
Identity = 210/313 (67.09%), Postives = 247/313 (78.91%), Query Frame = 0


Query: 1   MECIGSTQDLIQAPFPLN----CGAHA--AIRAPRPSANANLSFSLLGATAQTYKQFIND 60
           MEC+ S++  IQ P P N     G HA  A+ AP    N+N+SFSLLGAT +TY+QFI +
Sbjct: 1   MECVRSSEPFIQ-PLPFNDIGIHGVHANGAVNAP----NSNVSFSLLGATGKTYQQFIQN 60

Query: 61  LRKALTVNSHKVYDIPVLPATAKGLARFILVNLTNYKGESITVAIDTVDVYIVAYQAGNK 120
           LR ALT+NS  VY+IPVL ATA   ARFILV+LTNYK E+ITVAID V+VYIVAYQAGNK
Sbjct: 61  LRNALTINSKIVYNIPVLAATALSSARFILVHLTNYKNETITVAIDVVNVYIVAYQAGNK 120

Query: 121 AYFLKDVSKEAHDVLFKGSKHETLSYKGNYDDLERHAGKISRENIELGFSELSSSIGNMF 180
           AYFLKD SKEA DVLFKG K E L YKGNYD LE  AGKISRE I+LGFSEL S+IGNM+
Sbjct: 121 AYFLKDASKEARDVLFKGIKQEILPYKGNYDGLETAAGKISREKIDLGFSELGSAIGNMY 180

Query: 181 HYKAGYSVPRSFIVMIQMLSEASRFKYIETRNCEKVESKFKPDPGYLSLENRWSNLSEQV 240
           HY AG SVPR+FIVMIQ +SEA+RFKYIET+  + VE+KFKPDPG+LSLENRWS+LSEQV
Sbjct: 181 HYNAGTSVPRAFIVMIQTISEAARFKYIETKVSQNVETKFKPDPGFLSLENRWSDLSEQV 240

Query: 241 QKAQKHGGKFEHPVQVRSVSNKPVLVSDVNSPVVKGIALLLSNKPGVNG-VSKLMEEF-I 300
           Q AQ   G+F  P++VRSV+NKP+LV++V S VV+G+ALLL +KP  NG + +LMEEF  
Sbjct: 241 QIAQNRKGEFARPIEVRSVTNKPILVTNVKSKVVEGMALLLYSKPRGNGDLMELMEEFNK 300

Query: 301 HWEAARGHFGIDF 306
            WE  RG FGIDF
Sbjct: 301 EWENGRGEFGIDF 308

[Close]

BLAST of CmoCh01G010040 vs. ExPASy TrEMBL
Match: A0A6J1DVE7 (rRNA N-glycosidase OS=Momordica charantia OX=3673 GN=LOC111024729 PE=3 SV=1)

HSP 1 Score: 327.0 bits (837), Expect = 8.3e-86
Identity = 170/251 (67.73%), Postives = 205/251 (81.67%), Query Frame = 0


Query: 31  SANANLSFSLLGATAQTYKQFINDLRKALTVNSHKVYDIPVLPATAKGLARFILVNLTNY 90
           +A A++SFS+LG+T++TYKQFI ++R ALTV S  VYDIPVL ATA G ARF+LV+LTNY
Sbjct: 18  AAEADVSFSMLGSTSETYKQFIKNVRSALTVGSQIVYDIPVLKATATGSARFLLVHLTNY 77

Query: 91  KGESITVAIDTVDVYIVAYQAGNKAYFLKDVSKEAHDVLFKGSKHETLSYKGNYDDLERH 150
             ESI VAID V+VY+VAY+AGN AYFL D S EA  VLF+G  H  LSYKGNYD LE  
Sbjct: 78  NSESIIVAIDVVNVYVVAYRAGNNAYFLNDASAEADRVLFQGINHVKLSYKGNYDGLET- 137

Query: 151 AGKISRENIELGFSELSSSIGNMFHYKAGYSVPRSFIVMIQMLSEASRFKYIETRNCEKV 210
           A KISRE I+LGFSE+SSSIGNMFH+ AG SVPR+FIV+IQ +SEA+RFKYIE R  E V
Sbjct: 138 AAKISREKIDLGFSEISSSIGNMFHHNAGTSVPRAFIVIIQSVSEAARFKYIEQRVSENV 197

Query: 211 ESKFKPDPGYLSLENRWSNLSEQVQKAQKHGGKFEHPVQVRSVSNKPVLVSDVNSPVVKG 270
           ++ FKPDP +LSL+NRWS+LSEQ+Q AQK GG+F   V++R+VSNKP+LV+DV+SPVVKG
Sbjct: 198 KTTFKPDPAFLSLQNRWSDLSEQIQIAQKRGGEFARAVELRTVSNKPILVTDVSSPVVKG 257

Query: 271 IALLLSNKPGV 282
           IALLL  K  V
Sbjct: 258 IALLLYYKVSV 267

[Close]

BLAST of CmoCh01G010040 vs. ExPASy TrEMBL
Match: A0A6J1DWK4 (rRNA N-glycosidase OS=Momordica charantia OX=3673 GN=LOC111024728 PE=3 SV=1)

HSP 1 Score: 322.0 bits (824), Expect = 2.7e-84
Identity = 162/248 (65.32%), Postives = 203/248 (81.85%), Query Frame = 0


Query: 31  SANANLSFSLLGATAQTYKQFINDLRKALTVNSHKVYDIPVLPATAKGLARFILVNLTNY 90
           +A A++SFS+LGAT++TY+QFI +LR  LT+ S  VY IPVL +TA G ARFILV+LTNY
Sbjct: 18  AAEADVSFSMLGATSETYRQFIRNLRSTLTIRSPVVYGIPVLQSTAVGSARFILVHLTNY 77

Query: 91  KGESITVAIDTVDVYIVAYQAGNKAYFLKDVSKEAHDVLFKGSKHETLSYKGNYDDLERH 150
             ESITVAID V++Y+VAY+AGN AYFL D S EA++VLFKG KH  L YKGNYD LE  
Sbjct: 78  NTESITVAIDVVNIYVVAYRAGNNAYFLNDASAEANNVLFKGIKHVRLPYKGNYDGLETA 137

Query: 151 AGKISRENIELGFSELSSSIGNMFHYKAGYSVPRSFIVMIQMLSEASRFKYIETRNCEKV 210
           AGKISRE I+LGFSE+SSSIGNMFH+  G S+ R+FIV+IQ + EA+RFKYIE R  E V
Sbjct: 138 AGKISREKIDLGFSEISSSIGNMFHHNVGTSIARAFIVIIQSVLEAARFKYIEQRVSENV 197

Query: 211 ESKFKPDPGYLSLENRWSNLSEQVQKAQKHGGKFEHPVQVRSVSNKPVLVSDVNSPVVKG 270
           ++KFKPDP +LSLENRWS+LSEQ+Q AQK  G+F   +++R+VSNKP++V++V+SP+VKG
Sbjct: 198 KTKFKPDPAFLSLENRWSDLSEQIQIAQKREGEFARAIELRTVSNKPIIVTNVSSPIVKG 257

Query: 271 IALLLSNK 279
           IALLL  K
Sbjct: 258 IALLLYYK 265

The following BLAST results are available for this feature:

BLAST of CmoCh01G010040 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P98184	1.1e-82	62.16	Ribosome-inactivating protein bryodin II OS=Bryonia dioica OX=3652 PE=1 SV=1	[more]
P24817	3.6e-54	48.00	Ribosome-inactivating protein beta-momorcharin OS=Momordica charantia OX=3673 GN...	[more]
P29339	1.4e-53	47.60	Ribosome-inactivating protein momordin II OS=Momordica balsamina OX=3672 PE=3 SV...	[more]
P84531	4.1e-50	47.33	Ribosome-inactivating protein cucurmosin OS=Cucurbita moschata OX=3662 PE=1 SV=2	[more]
P09989	9.5e-47	44.40	Ribosome-inactivating protein alpha-trichosanthin OS=Trichosanthes kirilowii OX=...	[more]

BLAST of CmoCh01G010040 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1EKL0	1.1e-170	100.00	rRNA N-glycosidase OS=Cucurbita moschata OX=3662 GN=LOC111433434 PE=3 SV=1	[more]
A0A6J1IUB2	8.9e-165	96.39	rRNA N-glycosidase OS=Cucurbita maxima OX=3661 GN=LOC111480641 PE=3 SV=1	[more]
A0A6J1E093	3.0e-104	67.09	rRNA N-glycosidase OS=Momordica charantia OX=3673 GN=LOC111024726 PE=3 SV=1	[more]
A0A6J1DVE7	8.3e-86	67.73	rRNA N-glycosidase OS=Momordica charantia OX=3673 GN=LOC111024729 PE=3 SV=1	[more]
A0A6J1DWK4	2.7e-84	65.32	rRNA N-glycosidase OS=Momordica charantia OX=3673 GN=LOC111024728 PE=3 SV=1	[more]

BLAST of CmoCh01G010040 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description

InterPro

Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR017989	Ribosome-inactivating protein type 1/2	PRINTS	PR00396	SHIGARICIN	coord: 218..235 score: 43.57 coord: 94..109 score: 44.9 coord: 46..59 score: 42.29 coord: 156..170 score: 48.77 coord: 186..206 score: 53.88
IPR001574	Ribosome-inactivating protein	PFAM	PF00161	RIP	coord: 44..235 e-value: 2.5E-34 score: 118.6
IPR001574	Ribosome-inactivating protein	PANTHER	PTHR33453	FAMILY NOT NAMED	coord: 36..277
IPR016139	Ribosome-inactivating protein, subdomain 2	GENE3D	4.10.470.10	Ricin (A Subunit), domain 2	coord: 199..281 e-value: 1.3E-23 score: 84.6
IPR016138	Ribosome-inactivating protein, subdomain 1	GENE3D	3.40.420.10	Ricin (A subunit), domain 1	coord: 35..198 e-value: 3.1E-45 score: 155.9
None	No IPR available	PANTHER	PTHR33453:SF14	RRNA N-GLYCOSIDASE	coord: 36..277
IPR036041	Ribosome-inactivating protein superfamily	SUPERFAMILY	56371	Ribosome inactivating proteins (RIP)	coord: 36..276

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmoCh01G010040.1	CmoCh01G010040.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006952	defense response
biological_process	GO:0035821	modulation of process of other organism
biological_process	GO:0017148	negative regulation of translation
molecular_function	GO:0030598	rRNA N-glycosylase activity
molecular_function	GO:0090729	toxin activity