CmoCh01G009790 (gene) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh01G009790
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
Descriptionaspartic proteinase PCS1-like
LocationCmo_Chr01: 6394663 .. 6395925 (+)
RNA-Seq ExpressionCmoCh01G009790
SyntenyCmoCh01G009790
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCCTCTCTCTCTCCTACTGCTCTCCCTATTGGGTCTCTTATTCTCCCCATCAAACTCCCTCTCCCTCTCCTTCCCTCTGACCTCCCATTCTGTCTCCTCCCAAGAAGCCTCCCTTTCCCTCTCCTCCAAAACCAAATCCCATGGGAAGTTCCCCTTCCAGTACTCCAACGCCCTTGTGGTGTCTGTTCCCATTGGATCGCCGCCGCAGCAGATGGACATGGTCGTAGACACCGGCAGCCAACTGTCCTGGATTCAATGCCACGGCAAAGTAAGGAGAAAATCAGTGAAGCCCATGATTAATTGGTTTGACCCTTATCTCTCCTCCTCTTTCTCTTTCCTTCCCTGTAACACCACTCTGTGCAGACCCCGAATTCCCGATTTTACCCTTCCTACTTCTTGTGACCCAACTCGCCACTGCCACTACTCCTACTTCTACGCGGATGGGACCCTGGCCGAGGGTAATCTCGTCACTGAAAAATTCACCTTCTCCAATTCCTTAACTACCCGCTCCCTCCTTCTGGGCTGCGCTACGGCCTCCACCCAAAACAGGGGTATGTTGGGAATGAATACTGGACGCCTCTCCTTCATCTCCCAGGCCAAGATATCCAAGTTTTCCTATTGCGTTCCGGATCGAACCGGGTCGGATCTAACCGGCTTGTTCTACCTTGGAGACAACCCCAACTCCGCTAAATTCAAATACGTCAACATGTTGACTTTCCCCAAAAGTCGACTCTCCCCGAATCTTGACAAGTCGGCCTACACCCTCCCGATGAAGGGGATAAGAATAGGCAACAACAAACTCAACATCTCGCCCGCCGTTTTCAAACCGGACCCATCTGGGGCCGGTCAGACCATGATCGACTCCGGCTCCGATTTGACGTATTTGGTAGATGAGGCCTACAGCAAGGTCAGAGAAGAGATGGTCAGATTAGTGGGGCCCATGATGAAGAAAGGATACGAATACGCCGCCGTCGCCGACATGTGTTTCGACGGCGCAGTGGCGGCGGCGGTGGGTCGGAGGATCGGCGACATGTGGTTCCAGTTTGAGAATGGGGTGGAGATATTGGTCGGGAAAGGGGAAGGGTTATTGACGGAAGTGGAGGAAGGGGTGAAGTGCGTGGGGATCGGACGGTCAGATAGACTTGTGACTGAGAGTAATATAATCGGGAACGTTCATCAGCAGAATATGTGGGTGGAGTACGATCTGAGCAATAAGAGAATTGGGTTTGGTGTTGCCAAGTGTAGTGGATTGAAGGCATGA

mRNA sequence

ATGCCTCTCTCTCTCCTACTGCTCTCCCTATTGGGTCTCTTATTCTCCCCATCAAACTCCCTCTCCCTCTCCTTCCCTCTGACCTCCCATTCTGTCTCCTCCCAAGAAGCCTCCCTTTCCCTCTCCTCCAAAACCAAATCCCATGGGAAGTTCCCCTTCCAGTACTCCAACGCCCTTGTGGTGTCTGTTCCCATTGGATCGCCGCCGCAGCAGATGGACATGGTCGTAGACACCGGCAGCCAACTGTCCTGGATTCAATGCCACGGCAAAGTAAGGAGAAAATCAGTGAAGCCCATGATTAATTGGTTTGACCCTTATCTCTCCTCCTCTTTCTCTTTCCTTCCCTGTAACACCACTCTGTGCAGACCCCGAATTCCCGATTTTACCCTTCCTACTTCTTGTGACCCAACTCGCCACTGCCACTACTCCTACTTCTACGCGGATGGGACCCTGGCCGAGGGTAATCTCGTCACTGAAAAATTCACCTTCTCCAATTCCTTAACTACCCGCTCCCTCCTTCTGGGCTGCGCTACGGCCTCCACCCAAAACAGGGGTATGTTGGGAATGAATACTGGACGCCTCTCCTTCATCTCCCAGGCCAAGATATCCAAGTTTTCCTATTGCGTTCCGGATCGAACCGGGTCGGATCTAACCGGCTTGTTCTACCTTGGAGACAACCCCAACTCCGCTAAATTCAAATACGTCAACATGTTGACTTTCCCCAAAAGTCGACTCTCCCCGAATCTTGACAAGTCGGCCTACACCCTCCCGATGAAGGGGATAAGAATAGGCAACAACAAACTCAACATCTCGCCCGCCGTTTTCAAACCGGACCCATCTGGGGCCGGTCAGACCATGATCGACTCCGGCTCCGATTTGACGTATTTGGTAGATGAGGCCTACAGCAAGGTCAGAGAAGAGATGGTCAGATTAGTGGGGCCCATGATGAAGAAAGGATACGAATACGCCGCCGTCGCCGACATGTGTTTCGACGGCGCAGTGGCGGCGGCGGTGGGTCGGAGGATCGGCGACATGTGGTTCCAGTTTGAGAATGGGGTGGAGATATTGGTCGGGAAAGGGGAAGGGTTATTGACGGAAGTGGAGGAAGGGGTGAAGTGCGTGGGGATCGGACGGTCAGATAGACTTGTGACTGAGAGTAATATAATCGGGAACGTTCATCAGCAGAATATGTGGGTGGAGTACGATCTGAGCAATAAGAGAATTGGGTTTGGTGTTGCCAAGTGTAGTGGATTGAAGGCATGA

Coding sequence (CDS)

ATGCCTCTCTCTCTCCTACTGCTCTCCCTATTGGGTCTCTTATTCTCCCCATCAAACTCCCTCTCCCTCTCCTTCCCTCTGACCTCCCATTCTGTCTCCTCCCAAGAAGCCTCCCTTTCCCTCTCCTCCAAAACCAAATCCCATGGGAAGTTCCCCTTCCAGTACTCCAACGCCCTTGTGGTGTCTGTTCCCATTGGATCGCCGCCGCAGCAGATGGACATGGTCGTAGACACCGGCAGCCAACTGTCCTGGATTCAATGCCACGGCAAAGTAAGGAGAAAATCAGTGAAGCCCATGATTAATTGGTTTGACCCTTATCTCTCCTCCTCTTTCTCTTTCCTTCCCTGTAACACCACTCTGTGCAGACCCCGAATTCCCGATTTTACCCTTCCTACTTCTTGTGACCCAACTCGCCACTGCCACTACTCCTACTTCTACGCGGATGGGACCCTGGCCGAGGGTAATCTCGTCACTGAAAAATTCACCTTCTCCAATTCCTTAACTACCCGCTCCCTCCTTCTGGGCTGCGCTACGGCCTCCACCCAAAACAGGGGTATGTTGGGAATGAATACTGGACGCCTCTCCTTCATCTCCCAGGCCAAGATATCCAAGTTTTCCTATTGCGTTCCGGATCGAACCGGGTCGGATCTAACCGGCTTGTTCTACCTTGGAGACAACCCCAACTCCGCTAAATTCAAATACGTCAACATGTTGACTTTCCCCAAAAGTCGACTCTCCCCGAATCTTGACAAGTCGGCCTACACCCTCCCGATGAAGGGGATAAGAATAGGCAACAACAAACTCAACATCTCGCCCGCCGTTTTCAAACCGGACCCATCTGGGGCCGGTCAGACCATGATCGACTCCGGCTCCGATTTGACGTATTTGGTAGATGAGGCCTACAGCAAGGTCAGAGAAGAGATGGTCAGATTAGTGGGGCCCATGATGAAGAAAGGATACGAATACGCCGCCGTCGCCGACATGTGTTTCGACGGCGCAGTGGCGGCGGCGGTGGGTCGGAGGATCGGCGACATGTGGTTCCAGTTTGAGAATGGGGTGGAGATATTGGTCGGGAAAGGGGAAGGGTTATTGACGGAAGTGGAGGAAGGGGTGAAGTGCGTGGGGATCGGACGGTCAGATAGACTTGTGACTGAGAGTAATATAATCGGGAACGTTCATCAGCAGAATATGTGGGTGGAGTACGATCTGAGCAATAAGAGAATTGGGTTTGGTGTTGCCAAGTGTAGTGGATTGAAGGCATGA

Protein sequence

MPLSLLLLSLLGLLFSPSNSLSLSFPLTSHSVSSQEASLSLSSKTKSHGKFPFQYSNALVVSVPIGSPPQQMDMVVDTGSQLSWIQCHGKVRRKSVKPMINWFDPYLSSSFSFLPCNTTLCRPRIPDFTLPTSCDPTRHCHYSYFYADGTLAEGNLVTEKFTFSNSLTTRSLLLGCATASTQNRGMLGMNTGRLSFISQAKISKFSYCVPDRTGSDLTGLFYLGDNPNSAKFKYVNMLTFPKSRLSPNLDKSAYTLPMKGIRIGNNKLNISPAVFKPDPSGAGQTMIDSGSDLTYLVDEAYSKVREEMVRLVGPMMKKGYEYAAVADMCFDGAVAAAVGRRIGDMWFQFENGVEILVGKGEGLLTEVEEGVKCVGIGRSDRLVTESNIIGNVHQQNMWVEYDLSNKRIGFGVAKCSGLKA
Homology
BLAST of CmoCh01G009790 vs. ExPASy Swiss-Prot
Match: Q9LZL3 (Aspartic proteinase PCS1 OS=Arabidopsis thaliana OX=3702 GN=PCS1 PE=2 SV=1)

HSP 1 Score: 248.8 bits (634), Expect = 1.1e-64
Identity = 157/432 (36.34%), Postives = 228/432 (52.78%), Query Frame = 0

Query: 16  SPSNSLSLSFPLTSHSVSSQEASLSLSSKT-------KSHGKFPFQYSNALVVSVPIGSP 75
           S S+S S SF  +S S SS   +L L  KT       +   K  F ++  L V++ +G+P
Sbjct: 23  SSSSSSSSSFSFSSFSSSSSSQTLVLPLKTRITPTDHRPTDKLHFHHNVTLTVTLTVGTP 82

Query: 76  PQQMDMVVDTGSQLSWIQCHGKVRRKSVKPMINWFDPYLSSSFSFLPCNTTLCRPRIPDF 135
           PQ + MV+DTGS+LSW++C+    R S    +N FDP  SSS+S +PC++  CR R  DF
Sbjct: 83  PQNISMVIDTGSELSWLRCN----RSSNPNPVNNFDPTRSSSYSPIPCSSPTCRTRTRDF 142

Query: 136 TLPTSCDPTRHCHYSYFYADGTLAEGNLVTEKFTFSNSLTTRSLLLGCATA--------S 195
            +P SCD  + CH +  YAD + +EGNL  E F F NS    +L+ GC  +         
Sbjct: 143 LIPASCDSDKLCHATLSYADASSSEGNLAAEIFHFGNSTNDSNLIFGCMGSVSGSDPEED 202

Query: 196 TQNRGMLGMNTGRLSFISQAKISKFSYCVPDRTGSDLTGLFYLGDNPNSAKFKYVNMLTF 255
           T+  G+LGMN G LSFISQ    KFSYC+      D  G   LGD    + F ++  L +
Sbjct: 203 TKTTGLLGMNRGSLSFISQMGFPKFSYCISGT--DDFPGFLLLGD----SNFTWLTPLNY 262

Query: 256 -PKSRLS---PNLDKSAYTLPMKGIRIGNNKLNISPAVFKPDPSGAGQTMIDSGSDLTYL 315
            P  R+S   P  D+ AYT+ + GI++    L I  +V  PD +GAGQTM+DSG+  T+L
Sbjct: 263 TPLIRISTPLPYFDRVAYTVQLTGIKVNGKLLPIPKSVLVPDHTGAGQTMVDSGTQFTFL 322

Query: 316 VDEAYSKVREEMVRLVGPMM----KKGYEYAAVADMCF---DGAVAAAVGRRIGDMWFQF 375
           +   Y+ +R   +     ++       + +    D+C+      + + +  R+  +   F
Sbjct: 323 LGPVYTALRSHFLNRTNGILTVYEDPDFVFQGTMDLCYRISPVRIRSGILHRLPTVSLVF 382

Query: 376 ENGVEILVGKGEGLLTEV------EEGVKCVGIGRSDRLVTESNIIGNVHQQNMWVEYDL 416
           E G EI V  G+ LL  V       + V C   G SD +  E+ +IG+ HQQNMW+E+DL
Sbjct: 383 E-GAEIAV-SGQPLLYRVPHLTVGNDSVYCFTFGNSDLMGMEAYVIGHHHQQNMWIEFDL 442

BLAST of CmoCh01G009790 vs. ExPASy Swiss-Prot
Match: Q766C3 (Aspartic proteinase nepenthesin-1 OS=Nepenthes gracilis OX=150966 GN=nep1 PE=1 SV=1)

HSP 1 Score: 154.8 bits (390), Expect = 2.1e-36
Identity = 110/362 (30.39%), Postives = 171/362 (47.24%), Query Frame = 0

Query: 60  VVSVPIGSPPQQMDMVVDTGSQLSWIQCHGKVRRKSVKPMINWFDPYLSSSFSFLPCNTT 119
           ++++ IG+P Q    ++DTGS L W QC    +  +    I  F+P  SSSFS LPC++ 
Sbjct: 96  LMNLSIGTPAQPFSAIMDTGSDLIWTQCQPCTQCFNQSTPI--FNPQGSSSFSTLPCSSQ 155

Query: 120 LCRPRIPDFTLPTSCDPTRHCHYSYFYADGTLAEGNLVTEKFTFSNSLTTRSLLLGCAT- 179
           LC+       L +       C Y+Y Y DG+  +G++ TE  TF  S++  ++  GC   
Sbjct: 156 LCQ------ALSSPTCSNNFCQYTYGYGDGSETQGSMGTETLTF-GSVSIPNITFGCGEN 215

Query: 180 ----ASTQNRGMLGMNTGRLSFISQAKISKFSYCVPDRTGSDLTGLFYLGDNPNSAKFKY 239
                     G++GM  G LS  SQ  ++KFSYC+    GS       LG   NS     
Sbjct: 216 NQGFGQGNGAGLVGMGRGPLSLPSQLDVTKFSYCMTP-IGSSTPSNLLLGSLANSVTAGS 275

Query: 240 VNMLTFPKSRLSPNLDKSAYTLPMKGIRIGNNKLNISPAVFKPDP-SGAGQTMIDSGSDL 299
            N      S++      + Y + + G+ +G+ +L I P+ F  +  +G G  +IDSG+ L
Sbjct: 276 PNTTLIQSSQI-----PTFYYITLNGLSVGSTRLPIDPSAFALNSNNGTGGIIIDSGTTL 335

Query: 300 TYLVDEAYSKVREEMVRLVGPMMKKGYEYAAVADMCFDGAVAAAVGRRIGDMWFQFENGV 359
           TY V+ AY  VR+E +  +   +  G   ++  D+CF    +     +I      F+ G 
Sbjct: 336 TYFVNNAYQSVRQEFISQINLPVVNG--SSSGFDLCFQ-TPSDPSNLQIPTFVMHFDGG- 395

Query: 360 EILVGKGEGLLTEVEEGVKCVGIGRSDRLVTESNIIGNVHQQNMWVEYDLSNKRIGFGVA 416
             L    E        G+ C+ +G S +     +I GN+ QQNM V YD  N  + F  A
Sbjct: 396 -DLELPSENYFISPSNGLICLAMGSSSQ---GMSIFGNIQQQNMLVVYDTGNSVVSFASA 434

BLAST of CmoCh01G009790 vs. ExPASy Swiss-Prot
Match: Q766C2 (Aspartic proteinase nepenthesin-2 OS=Nepenthes gracilis OX=150966 GN=nep2 PE=1 SV=1)

HSP 1 Score: 151.0 bits (380), Expect = 3.0e-35
Identity = 112/365 (30.68%), Postives = 173/365 (47.40%), Query Frame = 0

Query: 60  VVSVPIGSPPQQMDMVVDTGSQLSWIQCHGKVRRKSVKPMINWFDPYLSSSFSFLPCNTT 119
           +++V IG+P      ++DTGS L W QC    +  S    I  F+P  SSSFS LPC + 
Sbjct: 97  LMNVAIGTPDSSFSAIMDTGSDLIWTQCEPCTQCFSQPTPI--FNPQDSSSFSTLPCESQ 156

Query: 120 LCRPRIPDFTLPTSCDPTRHCHYSYFYADGTLAEGNLVTEKFTFSNSLTTRSLLLGCAT- 179
            C+       LP+       C Y+Y Y DG+  +G + TE FTF  S +  ++  GC   
Sbjct: 157 YCQ------DLPSETCNNNECQYTYGYGDGSTTQGYMATETFTFETS-SVPNIAFGCGED 216

Query: 180 ----ASTQNRGMLGMNTGRLSFISQAKISKFSYCVPDRTGSDLTGLFYLGDNPNSAKFKY 239
                     G++GM  G LS  SQ  + +FSYC+    GS       LG   +    + 
Sbjct: 217 NQGFGQGNGAGLIGMGWGPLSLPSQLGVGQFSYCMTS-YGSSSPSTLALGSAASGVP-EG 276

Query: 240 VNMLTFPKSRLSPNLDKSAYTLPMKGIRIGNNKLNISPAVFKPDPSGAGQTMIDSGSDLT 299
               T   S L+P    + Y + ++GI +G + L I  + F+    G G  +IDSG+ LT
Sbjct: 277 SPSTTLIHSSLNP----TYYYITLQGITVGGDNLGIPSSTFQLQDDGTGGMIIDSGTTLT 336

Query: 300 YLVDEAYSKVREEMVRLVGPMMKKGYEYAAVADMCF----DGAVAAAVGRRIGDMWFQFE 359
           YL  +AY+ V +     +   +    E ++    CF    DG+       ++ ++  QF+
Sbjct: 337 YLPQDAYNAVAQAFTDQIN--LPTVDESSSGLSTCFQQPSDGSTV-----QVPEISMQFD 396

Query: 360 NGVEILVGKGEGLLTEVEEGVKCVGIGRSDRLVTESNIIGNVHQQNMWVEYDLSNKRIGF 416
            GV + +G+ + +L    EGV C+ +G S +L    +I GN+ QQ   V YDL N  + F
Sbjct: 397 GGV-LNLGE-QNILISPAEGVICLAMGSSSQL--GISIFGNIQQQETQVLYDLQNLAVSF 435

BLAST of CmoCh01G009790 vs. ExPASy Swiss-Prot
Match: Q9LS40 (Protein ASPARTIC PROTEASE IN GUARD CELL 1 OS=Arabidopsis thaliana OX=3702 GN=ASPG1 PE=1 SV=1)

HSP 1 Score: 146.0 bits (367), Expect = 9.8e-34
Identity = 105/357 (29.41%), Postives = 171/357 (47.90%), Query Frame = 0

Query: 63  VPIGSPPQQMDMVVDTGSQLSWIQCHGKVRRKSVKPMINWFDPYLSSSFSFLPCNTTLCR 122
           + +G+P ++M +V+DTGS ++WIQC            +  F+P  SS++  L C+   C 
Sbjct: 166 IGVGTPAKEMYLVLDTGSDVNWIQCEPCADCYQQSDPV--FNPTSSSTYKSLTCSAPQCS 225

Query: 123 PRIPDFTLPTSCDPTRHCHYSYFYADGTLAEGNLVTEKFTFSNSLTTRSLLLGCATAS-- 182
                  L TS   +  C Y   Y DG+   G L T+  TF NS    ++ LGC   +  
Sbjct: 226 ------LLETSACRSNKCLYQVSYGDGSFTVGELATDTVTFGNSGKINNVALGCGHDNEG 285

Query: 183 --TQNRGMLGMNTGRLSFISQAKISKFSYCVPDRTGSDLTGLFYLGDNPNSAKFKYVNML 242
             T   G+LG+  G LS  +Q K + FSYC+ DR     + L +     NS +    +  
Sbjct: 286 LFTGAAGLLGLGGGVLSITNQMKATSFSYCLVDRDSGKSSSLDF-----NSVQLGGGD-A 345

Query: 243 TFPKSRLSPNLDKSAYTLPMKGIRIGNNKLNISPAVFKPDPSGAGQTMIDSGSDLTYLVD 302
           T P  R +  +D + Y + + G  +G  K+ +  A+F  D SG+G  ++D G+ +T L  
Sbjct: 346 TAPLLR-NKKID-TFYYVGLSGFSVGGEKVVLPDAIFDVDASGSGGVILDCGTAVTRLQT 405

Query: 303 EAYSKVREEMVRLVGPMMKKGYEYAAVADMCFDGAVAAAVGRRIGDMWFQFENGVEILVG 362
           +AY+ +R+  ++L    +KKG    ++ D C+D +  + V  ++  + F F  G  + + 
Sbjct: 406 QAYNSLRDAFLKLT-VNLKKGSSSISLFDTCYDFSSLSTV--KVPTVAFHFTGGKSLDLP 465

Query: 363 KGEGLLTEVEEGVKCVGIGRSDRLVTESNIIGNVHQQNMWVEYDLSNKRIGFGVAKC 416
               L+   + G  C     +    +  +IIGNV QQ   + YDLS   IG    KC
Sbjct: 466 AKNYLIPVDDSGTFCFAFAPTS---SSLSIIGNVQQQGTRITYDLSKNVIGLSGNKC 500

BLAST of CmoCh01G009790 vs. ExPASy Swiss-Prot
Match: Q9LTW4 (Aspartic proteinase NANA, chloroplast OS=Arabidopsis thaliana OX=3702 GN=NANA PE=1 SV=1)

HSP 1 Score: 135.2 bits (339), Expect = 1.7e-30
Identity = 104/367 (28.34%), Postives = 172/367 (46.87%), Query Frame = 0

Query: 63  VPIGSPPQQMDMVVDTGSQLSWIQCHGKVRRKSVKPMINWFDPYLSSSFSFLPCNTTLCR 122
           + +G+P ++  +VVDTGS+L+W+ C  + R K  + +   F    S SF  + C T  C+
Sbjct: 110 IRVGTPAKKFRVVVDTGSELTWVNCRYRARGKDNRRV---FRADESKSFKTVGCLTQTCK 169

Query: 123 PRIPD-FTLPTSCDPTRHCHYSYFYADGTLAEGNLVTEKFT--FSNSLTTR--SLLLGCA 182
             + + F+L T   P+  C Y Y YADG+ A+G    E  T   +N    R    L+GC+
Sbjct: 170 VDLMNLFSLTTCPTPSTPCSYDYRYADGSAAQGVFAKETITVGLTNGRMARLPGHLIGCS 229

Query: 183 TASTQNR-----GMLGMNTGRLSFISQAKI---SKFSYCVPDRTGSDLTGLFYLGDNPNS 242
           ++ T        G+LG+     SF S A     +KFSYC+ D   +     + +  +  S
Sbjct: 230 SSFTGQSFQGADGVLGLAFSDFSFTSTATSLYGAKFSYCLVDHLSNKNVSNYLIFGSSRS 289

Query: 243 AKFKYVNMLTFPKSRLSPNLDKSAYTLPMKGIRIGNNKLNISPAVFKPDPSGAGQTMIDS 302
            K  +        +R+ P      Y + + GI +G + L+I   V+  D +  G T++DS
Sbjct: 290 TKTAFRRTTPLDLTRIPP-----FYAINVIGISLGYDMLDIPSQVW--DATSGGGTILDS 349

Query: 303 GSDLTYLVDEAYSKVREEMVRLVGPMMKKGYEYAAVADMCFDGAVAAAVGRRIGDMWFQF 362
           G+ LT L D AY +V   + R +  + +   E   + + CF       V  ++  + F  
Sbjct: 350 GTSLTLLADAAYKQVVTGLARYLVELKRVKPEGVPI-EYCFSFTSGFNVS-KLPQLTFHL 409

Query: 363 ENGVEILVGKGEGLLTEVEEGVKCVGIGRSDRLVTESNIIGNVHQQNMWVEYDLSNKRIG 417
           + G      + +  L +   GVKC+G   +    T  N+IGN+ QQN   E+DL    + 
Sbjct: 410 KGGARFEPHR-KSYLVDAAPGVKCLGFVSAGTPAT--NVIGNIMQQNYLWEFDLMASTLS 461

BLAST of CmoCh01G009790 vs. ExPASy TrEMBL
Match: A0A6J1EWZ6 (aspartic proteinase PCS1-like OS=Cucurbita moschata OX=3662 GN=LOC111438863 PE=3 SV=1)

HSP 1 Score: 840.1 bits (2169), Expect = 4.0e-240
Identity = 420/420 (100.00%), Postives = 420/420 (100.00%), Query Frame = 0

Query: 1   MPLSLLLLSLLGLLFSPSNSLSLSFPLTSHSVSSQEASLSLSSKTKSHGKFPFQYSNALV 60
           MPLSLLLLSLLGLLFSPSNSLSLSFPLTSHSVSSQEASLSLSSKTKSHGKFPFQYSNALV
Sbjct: 1   MPLSLLLLSLLGLLFSPSNSLSLSFPLTSHSVSSQEASLSLSSKTKSHGKFPFQYSNALV 60

Query: 61  VSVPIGSPPQQMDMVVDTGSQLSWIQCHGKVRRKSVKPMINWFDPYLSSSFSFLPCNTTL 120
           VSVPIGSPPQQMDMVVDTGSQLSWIQCHGKVRRKSVKPMINWFDPYLSSSFSFLPCNTTL
Sbjct: 61  VSVPIGSPPQQMDMVVDTGSQLSWIQCHGKVRRKSVKPMINWFDPYLSSSFSFLPCNTTL 120

Query: 121 CRPRIPDFTLPTSCDPTRHCHYSYFYADGTLAEGNLVTEKFTFSNSLTTRSLLLGCATAS 180
           CRPRIPDFTLPTSCDPTRHCHYSYFYADGTLAEGNLVTEKFTFSNSLTTRSLLLGCATAS
Sbjct: 121 CRPRIPDFTLPTSCDPTRHCHYSYFYADGTLAEGNLVTEKFTFSNSLTTRSLLLGCATAS 180

Query: 181 TQNRGMLGMNTGRLSFISQAKISKFSYCVPDRTGSDLTGLFYLGDNPNSAKFKYVNMLTF 240
           TQNRGMLGMNTGRLSFISQAKISKFSYCVPDRTGSDLTGLFYLGDNPNSAKFKYVNMLTF
Sbjct: 181 TQNRGMLGMNTGRLSFISQAKISKFSYCVPDRTGSDLTGLFYLGDNPNSAKFKYVNMLTF 240

Query: 241 PKSRLSPNLDKSAYTLPMKGIRIGNNKLNISPAVFKPDPSGAGQTMIDSGSDLTYLVDEA 300
           PKSRLSPNLDKSAYTLPMKGIRIGNNKLNISPAVFKPDPSGAGQTMIDSGSDLTYLVDEA
Sbjct: 241 PKSRLSPNLDKSAYTLPMKGIRIGNNKLNISPAVFKPDPSGAGQTMIDSGSDLTYLVDEA 300

Query: 301 YSKVREEMVRLVGPMMKKGYEYAAVADMCFDGAVAAAVGRRIGDMWFQFENGVEILVGKG 360
           YSKVREEMVRLVGPMMKKGYEYAAVADMCFDGAVAAAVGRRIGDMWFQFENGVEILVGKG
Sbjct: 301 YSKVREEMVRLVGPMMKKGYEYAAVADMCFDGAVAAAVGRRIGDMWFQFENGVEILVGKG 360

Query: 361 EGLLTEVEEGVKCVGIGRSDRLVTESNIIGNVHQQNMWVEYDLSNKRIGFGVAKCSGLKA 420
           EGLLTEVEEGVKCVGIGRSDRLVTESNIIGNVHQQNMWVEYDLSNKRIGFGVAKCSGLKA
Sbjct: 361 EGLLTEVEEGVKCVGIGRSDRLVTESNIIGNVHQQNMWVEYDLSNKRIGFGVAKCSGLKA 420

BLAST of CmoCh01G009790 vs. ExPASy TrEMBL
Match: A0A6J1F0M7 (aspartic proteinase PCS1-like OS=Cucurbita moschata OX=3662 GN=LOC111438404 PE=3 SV=1)

HSP 1 Score: 839.7 bits (2168), Expect = 5.2e-240
Identity = 419/420 (99.76%), Postives = 420/420 (100.00%), Query Frame = 0

Query: 1   MPLSLLLLSLLGLLFSPSNSLSLSFPLTSHSVSSQEASLSLSSKTKSHGKFPFQYSNALV 60
           MPLSLLLLSLLGLLFSPSNSLSLSFPLTSHSVSSQEASLSLSSKTKSHGKFPFQYSNALV
Sbjct: 1   MPLSLLLLSLLGLLFSPSNSLSLSFPLTSHSVSSQEASLSLSSKTKSHGKFPFQYSNALV 60

Query: 61  VSVPIGSPPQQMDMVVDTGSQLSWIQCHGKVRRKSVKPMINWFDPYLSSSFSFLPCNTTL 120
           VSVPIGSPPQQMDMVVDTGSQLSWIQCHGKVRRKSVKPMINWFDPYLSSSFSFLPCNTTL
Sbjct: 61  VSVPIGSPPQQMDMVVDTGSQLSWIQCHGKVRRKSVKPMINWFDPYLSSSFSFLPCNTTL 120

Query: 121 CRPRIPDFTLPTSCDPTRHCHYSYFYADGTLAEGNLVTEKFTFSNSLTTRSLLLGCATAS 180
           CRPRIPDFTLPTSCDPTRHCHYSYFYADGTLAEGNLVTEKFTFSNSLTTRSLLLGCATAS
Sbjct: 121 CRPRIPDFTLPTSCDPTRHCHYSYFYADGTLAEGNLVTEKFTFSNSLTTRSLLLGCATAS 180

Query: 181 TQNRGMLGMNTGRLSFISQAKISKFSYCVPDRTGSDLTGLFYLGDNPNSAKFKYVNMLTF 240
           TQNRGMLGMNTGRLSFISQAKISKFSYCVPDRTGSDLTGLFYLGDNPNSAKFKYVNMLTF
Sbjct: 181 TQNRGMLGMNTGRLSFISQAKISKFSYCVPDRTGSDLTGLFYLGDNPNSAKFKYVNMLTF 240

Query: 241 PKSRLSPNLDKSAYTLPMKGIRIGNNKLNISPAVFKPDPSGAGQTMIDSGSDLTYLVDEA 300
           PKSRLSPNLDKSAYTLPMKGIRIGNNKLNISPA+FKPDPSGAGQTMIDSGSDLTYLVDEA
Sbjct: 241 PKSRLSPNLDKSAYTLPMKGIRIGNNKLNISPAIFKPDPSGAGQTMIDSGSDLTYLVDEA 300

Query: 301 YSKVREEMVRLVGPMMKKGYEYAAVADMCFDGAVAAAVGRRIGDMWFQFENGVEILVGKG 360
           YSKVREEMVRLVGPMMKKGYEYAAVADMCFDGAVAAAVGRRIGDMWFQFENGVEILVGKG
Sbjct: 301 YSKVREEMVRLVGPMMKKGYEYAAVADMCFDGAVAAAVGRRIGDMWFQFENGVEILVGKG 360

Query: 361 EGLLTEVEEGVKCVGIGRSDRLVTESNIIGNVHQQNMWVEYDLSNKRIGFGVAKCSGLKA 420
           EGLLTEVEEGVKCVGIGRSDRLVTESNIIGNVHQQNMWVEYDLSNKRIGFGVAKCSGLKA
Sbjct: 361 EGLLTEVEEGVKCVGIGRSDRLVTESNIIGNVHQQNMWVEYDLSNKRIGFGVAKCSGLKA 420

BLAST of CmoCh01G009790 vs. ExPASy TrEMBL
Match: A0A6J1F181 (aspartic proteinase PCS1-like OS=Cucurbita moschata OX=3662 GN=LOC111438406 PE=3 SV=1)

HSP 1 Score: 838.6 bits (2165), Expect = 1.2e-239
Identity = 419/420 (99.76%), Postives = 420/420 (100.00%), Query Frame = 0

Query: 1   MPLSLLLLSLLGLLFSPSNSLSLSFPLTSHSVSSQEASLSLSSKTKSHGKFPFQYSNALV 60
           MPLSLLLLSLLGLLFSPSNSLSLSFPLTSHSVSS+EASLSLSSKTKSHGKFPFQYSNALV
Sbjct: 1   MPLSLLLLSLLGLLFSPSNSLSLSFPLTSHSVSSREASLSLSSKTKSHGKFPFQYSNALV 60

Query: 61  VSVPIGSPPQQMDMVVDTGSQLSWIQCHGKVRRKSVKPMINWFDPYLSSSFSFLPCNTTL 120
           VSVPIGSPPQQMDMVVDTGSQLSWIQCHGKVRRKSVKPMINWFDPYLSSSFSFLPCNTTL
Sbjct: 61  VSVPIGSPPQQMDMVVDTGSQLSWIQCHGKVRRKSVKPMINWFDPYLSSSFSFLPCNTTL 120

Query: 121 CRPRIPDFTLPTSCDPTRHCHYSYFYADGTLAEGNLVTEKFTFSNSLTTRSLLLGCATAS 180
           CRPRIPDFTLPTSCDPTRHCHYSYFYADGTLAEGNLVTEKFTFSNSLTTRSLLLGCATAS
Sbjct: 121 CRPRIPDFTLPTSCDPTRHCHYSYFYADGTLAEGNLVTEKFTFSNSLTTRSLLLGCATAS 180

Query: 181 TQNRGMLGMNTGRLSFISQAKISKFSYCVPDRTGSDLTGLFYLGDNPNSAKFKYVNMLTF 240
           TQNRGMLGMNTGRLSFISQAKISKFSYCVPDRTGSDLTGLFYLGDNPNSAKFKYVNMLTF
Sbjct: 181 TQNRGMLGMNTGRLSFISQAKISKFSYCVPDRTGSDLTGLFYLGDNPNSAKFKYVNMLTF 240

Query: 241 PKSRLSPNLDKSAYTLPMKGIRIGNNKLNISPAVFKPDPSGAGQTMIDSGSDLTYLVDEA 300
           PKSRLSPNLDKSAYTLPMKGIRIGNNKLNISPAVFKPDPSGAGQTMIDSGSDLTYLVDEA
Sbjct: 241 PKSRLSPNLDKSAYTLPMKGIRIGNNKLNISPAVFKPDPSGAGQTMIDSGSDLTYLVDEA 300

Query: 301 YSKVREEMVRLVGPMMKKGYEYAAVADMCFDGAVAAAVGRRIGDMWFQFENGVEILVGKG 360
           YSKVREEMVRLVGPMMKKGYEYAAVADMCFDGAVAAAVGRRIGDMWFQFENGVEILVGKG
Sbjct: 301 YSKVREEMVRLVGPMMKKGYEYAAVADMCFDGAVAAAVGRRIGDMWFQFENGVEILVGKG 360

Query: 361 EGLLTEVEEGVKCVGIGRSDRLVTESNIIGNVHQQNMWVEYDLSNKRIGFGVAKCSGLKA 420
           EGLLTEVEEGVKCVGIGRSDRLVTESNIIGNVHQQNMWVEYDLSNKRIGFGVAKCSGLKA
Sbjct: 361 EGLLTEVEEGVKCVGIGRSDRLVTESNIIGNVHQQNMWVEYDLSNKRIGFGVAKCSGLKA 420

BLAST of CmoCh01G009790 vs. ExPASy TrEMBL
Match: A0A6J1F1P3 (aspartic proteinase PCS1-like OS=Cucurbita moschata OX=3662 GN=LOC111438856 PE=3 SV=1)

HSP 1 Score: 837.8 bits (2163), Expect = 2.0e-239
Identity = 419/420 (99.76%), Postives = 419/420 (99.76%), Query Frame = 0

Query: 1   MPLSLLLLSLLGLLFSPSNSLSLSFPLTSHSVSSQEASLSLSSKTKSHGKFPFQYSNALV 60
           MPLSLLLLSLLGLLFSPSNSLSLSFPLTSHSVSSQEASLSLS KTKSHGKFPFQYSNALV
Sbjct: 1   MPLSLLLLSLLGLLFSPSNSLSLSFPLTSHSVSSQEASLSLSIKTKSHGKFPFQYSNALV 60

Query: 61  VSVPIGSPPQQMDMVVDTGSQLSWIQCHGKVRRKSVKPMINWFDPYLSSSFSFLPCNTTL 120
           VSVPIGSPPQQMDMVVDTGSQLSWIQCHGKVRRKSVKPMINWFDPYLSSSFSFLPCNTTL
Sbjct: 61  VSVPIGSPPQQMDMVVDTGSQLSWIQCHGKVRRKSVKPMINWFDPYLSSSFSFLPCNTTL 120

Query: 121 CRPRIPDFTLPTSCDPTRHCHYSYFYADGTLAEGNLVTEKFTFSNSLTTRSLLLGCATAS 180
           CRPRIPDFTLPTSCDPTRHCHYSYFYADGTLAEGNLVTEKFTFSNSLTTRSLLLGCATAS
Sbjct: 121 CRPRIPDFTLPTSCDPTRHCHYSYFYADGTLAEGNLVTEKFTFSNSLTTRSLLLGCATAS 180

Query: 181 TQNRGMLGMNTGRLSFISQAKISKFSYCVPDRTGSDLTGLFYLGDNPNSAKFKYVNMLTF 240
           TQNRGMLGMNTGRLSFISQAKISKFSYCVPDRTGSDLTGLFYLGDNPNSAKFKYVNMLTF
Sbjct: 181 TQNRGMLGMNTGRLSFISQAKISKFSYCVPDRTGSDLTGLFYLGDNPNSAKFKYVNMLTF 240

Query: 241 PKSRLSPNLDKSAYTLPMKGIRIGNNKLNISPAVFKPDPSGAGQTMIDSGSDLTYLVDEA 300
           PKSRLSPNLDKSAYTLPMKGIRIGNNKLNISPAVFKPDPSGAGQTMIDSGSDLTYLVDEA
Sbjct: 241 PKSRLSPNLDKSAYTLPMKGIRIGNNKLNISPAVFKPDPSGAGQTMIDSGSDLTYLVDEA 300

Query: 301 YSKVREEMVRLVGPMMKKGYEYAAVADMCFDGAVAAAVGRRIGDMWFQFENGVEILVGKG 360
           YSKVREEMVRLVGPMMKKGYEYAAVADMCFDGAVAAAVGRRIGDMWFQFENGVEILVGKG
Sbjct: 301 YSKVREEMVRLVGPMMKKGYEYAAVADMCFDGAVAAAVGRRIGDMWFQFENGVEILVGKG 360

Query: 361 EGLLTEVEEGVKCVGIGRSDRLVTESNIIGNVHQQNMWVEYDLSNKRIGFGVAKCSGLKA 420
           EGLLTEVEEGVKCVGIGRSDRLVTESNIIGNVHQQNMWVEYDLSNKRIGFGVAKCSGLKA
Sbjct: 361 EGLLTEVEEGVKCVGIGRSDRLVTESNIIGNVHQQNMWVEYDLSNKRIGFGVAKCSGLKA 420

BLAST of CmoCh01G009790 vs. ExPASy TrEMBL
Match: A0A6J1I612 (aspartic proteinase PCS1-like OS=Cucurbita maxima OX=3661 GN=LOC111471392 PE=3 SV=1)

HSP 1 Score: 820.8 bits (2119), Expect = 2.5e-234
Identity = 410/420 (97.62%), Postives = 413/420 (98.33%), Query Frame = 0

Query: 1   MPLSLLLLSLLGLLFSPSNSLSLSFPLTSHSVSSQEASLSLSSKTKSHGKFPFQYSNALV 60
           MPLSLLLLSL G+LFSPSNSLSLSFPLTSHSVSSQEASLSLSSKTKSHGK PFQYSNALV
Sbjct: 1   MPLSLLLLSLFGVLFSPSNSLSLSFPLTSHSVSSQEASLSLSSKTKSHGKLPFQYSNALV 60

Query: 61  VSVPIGSPPQQMDMVVDTGSQLSWIQCHGKVRRKSVKPMINWFDPYLSSSFSFLPCNTTL 120
           VSVPIGSPPQQMDMVVDTGSQLSWIQCHGKVRRKSVKPMINWFDPYLSSSFSFLPCNTTL
Sbjct: 61  VSVPIGSPPQQMDMVVDTGSQLSWIQCHGKVRRKSVKPMINWFDPYLSSSFSFLPCNTTL 120

Query: 121 CRPRIPDFTLPTSCDPTRHCHYSYFYADGTLAEGNLVTEKFTFSNSLTTRSLLLGCATAS 180
           CRPRIPDFTLPTSCDPTRHCHYSYFYADGTLAEGNLVTEKFTFS+SLTTRSLLLGCATAS
Sbjct: 121 CRPRIPDFTLPTSCDPTRHCHYSYFYADGTLAEGNLVTEKFTFSSSLTTRSLLLGCATAS 180

Query: 181 TQNRGMLGMNTGRLSFISQAKISKFSYCVPDRTGSDLTGLFYLGDNPNSAKFKYVNMLTF 240
           TQNRGMLGMNTGRLSFISQAKISKFSYCVPDRTGSDLTGLFYLGDNPNSAKFKYVNMLTF
Sbjct: 181 TQNRGMLGMNTGRLSFISQAKISKFSYCVPDRTGSDLTGLFYLGDNPNSAKFKYVNMLTF 240

Query: 241 PKSRLSPNLDKSAYTLPMKGIRIGNNKLNISPAVFKPDPSGAGQTMIDSGSDLTYLVDEA 300
           PKSR SPNLDK AYTLPMKGIRIGNNKLNISPAVFKPDPSGAGQTMIDSGSDLTYLVDEA
Sbjct: 241 PKSRRSPNLDKLAYTLPMKGIRIGNNKLNISPAVFKPDPSGAGQTMIDSGSDLTYLVDEA 300

Query: 301 YSKVREEMVRLVGPMMKKGYEYAAVADMCFDGAVAAAVGRRIGDMWFQFENGVEILVGKG 360
           YSKVR EMVRLVGPMMKKGYEYAAVADMCFDGAVAA VGRRIGDMWFQFENGVEILVGKG
Sbjct: 301 YSKVRAEMVRLVGPMMKKGYEYAAVADMCFDGAVAAVVGRRIGDMWFQFENGVEILVGKG 360

Query: 361 EGLLTEVEEGVKCVGIGRSDRLVTESNIIGNVHQQNMWVEYDLSNKRIGFGVAKCSGLKA 420
           EGLLTEVE+GVKCVGIGRSDRLVTESNIIGNVHQQNMWVEYDLSNKRIGFGVA CSGLKA
Sbjct: 361 EGLLTEVEKGVKCVGIGRSDRLVTESNIIGNVHQQNMWVEYDLSNKRIGFGVAMCSGLKA 420

BLAST of CmoCh01G009790 vs. NCBI nr
Match: XP_022932084.1 (aspartic proteinase PCS1-like [Cucurbita moschata] >XP_022932086.1 aspartic proteinase PCS1-like [Cucurbita moschata] >XP_022932444.1 aspartic proteinase PCS1-like [Cucurbita moschata] >XP_022933293.1 aspartic proteinase PCS1-like [Cucurbita moschata] >XP_022933314.1 aspartic proteinase PCS1-like [Cucurbita moschata])

HSP 1 Score: 840.1 bits (2169), Expect = 8.3e-240
Identity = 420/420 (100.00%), Postives = 420/420 (100.00%), Query Frame = 0

Query: 1   MPLSLLLLSLLGLLFSPSNSLSLSFPLTSHSVSSQEASLSLSSKTKSHGKFPFQYSNALV 60
           MPLSLLLLSLLGLLFSPSNSLSLSFPLTSHSVSSQEASLSLSSKTKSHGKFPFQYSNALV
Sbjct: 1   MPLSLLLLSLLGLLFSPSNSLSLSFPLTSHSVSSQEASLSLSSKTKSHGKFPFQYSNALV 60

Query: 61  VSVPIGSPPQQMDMVVDTGSQLSWIQCHGKVRRKSVKPMINWFDPYLSSSFSFLPCNTTL 120
           VSVPIGSPPQQMDMVVDTGSQLSWIQCHGKVRRKSVKPMINWFDPYLSSSFSFLPCNTTL
Sbjct: 61  VSVPIGSPPQQMDMVVDTGSQLSWIQCHGKVRRKSVKPMINWFDPYLSSSFSFLPCNTTL 120

Query: 121 CRPRIPDFTLPTSCDPTRHCHYSYFYADGTLAEGNLVTEKFTFSNSLTTRSLLLGCATAS 180
           CRPRIPDFTLPTSCDPTRHCHYSYFYADGTLAEGNLVTEKFTFSNSLTTRSLLLGCATAS
Sbjct: 121 CRPRIPDFTLPTSCDPTRHCHYSYFYADGTLAEGNLVTEKFTFSNSLTTRSLLLGCATAS 180

Query: 181 TQNRGMLGMNTGRLSFISQAKISKFSYCVPDRTGSDLTGLFYLGDNPNSAKFKYVNMLTF 240
           TQNRGMLGMNTGRLSFISQAKISKFSYCVPDRTGSDLTGLFYLGDNPNSAKFKYVNMLTF
Sbjct: 181 TQNRGMLGMNTGRLSFISQAKISKFSYCVPDRTGSDLTGLFYLGDNPNSAKFKYVNMLTF 240

Query: 241 PKSRLSPNLDKSAYTLPMKGIRIGNNKLNISPAVFKPDPSGAGQTMIDSGSDLTYLVDEA 300
           PKSRLSPNLDKSAYTLPMKGIRIGNNKLNISPAVFKPDPSGAGQTMIDSGSDLTYLVDEA
Sbjct: 241 PKSRLSPNLDKSAYTLPMKGIRIGNNKLNISPAVFKPDPSGAGQTMIDSGSDLTYLVDEA 300

Query: 301 YSKVREEMVRLVGPMMKKGYEYAAVADMCFDGAVAAAVGRRIGDMWFQFENGVEILVGKG 360
           YSKVREEMVRLVGPMMKKGYEYAAVADMCFDGAVAAAVGRRIGDMWFQFENGVEILVGKG
Sbjct: 301 YSKVREEMVRLVGPMMKKGYEYAAVADMCFDGAVAAAVGRRIGDMWFQFENGVEILVGKG 360

Query: 361 EGLLTEVEEGVKCVGIGRSDRLVTESNIIGNVHQQNMWVEYDLSNKRIGFGVAKCSGLKA 420
           EGLLTEVEEGVKCVGIGRSDRLVTESNIIGNVHQQNMWVEYDLSNKRIGFGVAKCSGLKA
Sbjct: 361 EGLLTEVEEGVKCVGIGRSDRLVTESNIIGNVHQQNMWVEYDLSNKRIGFGVAKCSGLKA 420

BLAST of CmoCh01G009790 vs. NCBI nr
Match: XP_022932083.1 (aspartic proteinase PCS1-like [Cucurbita moschata])

HSP 1 Score: 839.7 bits (2168), Expect = 1.1e-239
Identity = 419/420 (99.76%), Postives = 420/420 (100.00%), Query Frame = 0

Query: 1   MPLSLLLLSLLGLLFSPSNSLSLSFPLTSHSVSSQEASLSLSSKTKSHGKFPFQYSNALV 60
           MPLSLLLLSLLGLLFSPSNSLSLSFPLTSHSVSSQEASLSLSSKTKSHGKFPFQYSNALV
Sbjct: 1   MPLSLLLLSLLGLLFSPSNSLSLSFPLTSHSVSSQEASLSLSSKTKSHGKFPFQYSNALV 60

Query: 61  VSVPIGSPPQQMDMVVDTGSQLSWIQCHGKVRRKSVKPMINWFDPYLSSSFSFLPCNTTL 120
           VSVPIGSPPQQMDMVVDTGSQLSWIQCHGKVRRKSVKPMINWFDPYLSSSFSFLPCNTTL
Sbjct: 61  VSVPIGSPPQQMDMVVDTGSQLSWIQCHGKVRRKSVKPMINWFDPYLSSSFSFLPCNTTL 120

Query: 121 CRPRIPDFTLPTSCDPTRHCHYSYFYADGTLAEGNLVTEKFTFSNSLTTRSLLLGCATAS 180
           CRPRIPDFTLPTSCDPTRHCHYSYFYADGTLAEGNLVTEKFTFSNSLTTRSLLLGCATAS
Sbjct: 121 CRPRIPDFTLPTSCDPTRHCHYSYFYADGTLAEGNLVTEKFTFSNSLTTRSLLLGCATAS 180

Query: 181 TQNRGMLGMNTGRLSFISQAKISKFSYCVPDRTGSDLTGLFYLGDNPNSAKFKYVNMLTF 240
           TQNRGMLGMNTGRLSFISQAKISKFSYCVPDRTGSDLTGLFYLGDNPNSAKFKYVNMLTF
Sbjct: 181 TQNRGMLGMNTGRLSFISQAKISKFSYCVPDRTGSDLTGLFYLGDNPNSAKFKYVNMLTF 240

Query: 241 PKSRLSPNLDKSAYTLPMKGIRIGNNKLNISPAVFKPDPSGAGQTMIDSGSDLTYLVDEA 300
           PKSRLSPNLDKSAYTLPMKGIRIGNNKLNISPA+FKPDPSGAGQTMIDSGSDLTYLVDEA
Sbjct: 241 PKSRLSPNLDKSAYTLPMKGIRIGNNKLNISPAIFKPDPSGAGQTMIDSGSDLTYLVDEA 300

Query: 301 YSKVREEMVRLVGPMMKKGYEYAAVADMCFDGAVAAAVGRRIGDMWFQFENGVEILVGKG 360
           YSKVREEMVRLVGPMMKKGYEYAAVADMCFDGAVAAAVGRRIGDMWFQFENGVEILVGKG
Sbjct: 301 YSKVREEMVRLVGPMMKKGYEYAAVADMCFDGAVAAAVGRRIGDMWFQFENGVEILVGKG 360

Query: 361 EGLLTEVEEGVKCVGIGRSDRLVTESNIIGNVHQQNMWVEYDLSNKRIGFGVAKCSGLKA 420
           EGLLTEVEEGVKCVGIGRSDRLVTESNIIGNVHQQNMWVEYDLSNKRIGFGVAKCSGLKA
Sbjct: 361 EGLLTEVEEGVKCVGIGRSDRLVTESNIIGNVHQQNMWVEYDLSNKRIGFGVAKCSGLKA 420

BLAST of CmoCh01G009790 vs. NCBI nr
Match: XP_022932085.1 (aspartic proteinase PCS1-like [Cucurbita moschata])

HSP 1 Score: 838.6 bits (2165), Expect = 2.4e-239
Identity = 419/420 (99.76%), Postives = 420/420 (100.00%), Query Frame = 0

Query: 1   MPLSLLLLSLLGLLFSPSNSLSLSFPLTSHSVSSQEASLSLSSKTKSHGKFPFQYSNALV 60
           MPLSLLLLSLLGLLFSPSNSLSLSFPLTSHSVSS+EASLSLSSKTKSHGKFPFQYSNALV
Sbjct: 1   MPLSLLLLSLLGLLFSPSNSLSLSFPLTSHSVSSREASLSLSSKTKSHGKFPFQYSNALV 60

Query: 61  VSVPIGSPPQQMDMVVDTGSQLSWIQCHGKVRRKSVKPMINWFDPYLSSSFSFLPCNTTL 120
           VSVPIGSPPQQMDMVVDTGSQLSWIQCHGKVRRKSVKPMINWFDPYLSSSFSFLPCNTTL
Sbjct: 61  VSVPIGSPPQQMDMVVDTGSQLSWIQCHGKVRRKSVKPMINWFDPYLSSSFSFLPCNTTL 120

Query: 121 CRPRIPDFTLPTSCDPTRHCHYSYFYADGTLAEGNLVTEKFTFSNSLTTRSLLLGCATAS 180
           CRPRIPDFTLPTSCDPTRHCHYSYFYADGTLAEGNLVTEKFTFSNSLTTRSLLLGCATAS
Sbjct: 121 CRPRIPDFTLPTSCDPTRHCHYSYFYADGTLAEGNLVTEKFTFSNSLTTRSLLLGCATAS 180

Query: 181 TQNRGMLGMNTGRLSFISQAKISKFSYCVPDRTGSDLTGLFYLGDNPNSAKFKYVNMLTF 240
           TQNRGMLGMNTGRLSFISQAKISKFSYCVPDRTGSDLTGLFYLGDNPNSAKFKYVNMLTF
Sbjct: 181 TQNRGMLGMNTGRLSFISQAKISKFSYCVPDRTGSDLTGLFYLGDNPNSAKFKYVNMLTF 240

Query: 241 PKSRLSPNLDKSAYTLPMKGIRIGNNKLNISPAVFKPDPSGAGQTMIDSGSDLTYLVDEA 300
           PKSRLSPNLDKSAYTLPMKGIRIGNNKLNISPAVFKPDPSGAGQTMIDSGSDLTYLVDEA
Sbjct: 241 PKSRLSPNLDKSAYTLPMKGIRIGNNKLNISPAVFKPDPSGAGQTMIDSGSDLTYLVDEA 300

Query: 301 YSKVREEMVRLVGPMMKKGYEYAAVADMCFDGAVAAAVGRRIGDMWFQFENGVEILVGKG 360
           YSKVREEMVRLVGPMMKKGYEYAAVADMCFDGAVAAAVGRRIGDMWFQFENGVEILVGKG
Sbjct: 301 YSKVREEMVRLVGPMMKKGYEYAAVADMCFDGAVAAAVGRRIGDMWFQFENGVEILVGKG 360

Query: 361 EGLLTEVEEGVKCVGIGRSDRLVTESNIIGNVHQQNMWVEYDLSNKRIGFGVAKCSGLKA 420
           EGLLTEVEEGVKCVGIGRSDRLVTESNIIGNVHQQNMWVEYDLSNKRIGFGVAKCSGLKA
Sbjct: 361 EGLLTEVEEGVKCVGIGRSDRLVTESNIIGNVHQQNMWVEYDLSNKRIGFGVAKCSGLKA 420

BLAST of CmoCh01G009790 vs. NCBI nr
Match: XP_022932433.1 (aspartic proteinase PCS1-like [Cucurbita moschata])

HSP 1 Score: 837.8 bits (2163), Expect = 4.1e-239
Identity = 419/420 (99.76%), Postives = 419/420 (99.76%), Query Frame = 0

Query: 1   MPLSLLLLSLLGLLFSPSNSLSLSFPLTSHSVSSQEASLSLSSKTKSHGKFPFQYSNALV 60
           MPLSLLLLSLLGLLFSPSNSLSLSFPLTSHSVSSQEASLSLS KTKSHGKFPFQYSNALV
Sbjct: 1   MPLSLLLLSLLGLLFSPSNSLSLSFPLTSHSVSSQEASLSLSIKTKSHGKFPFQYSNALV 60

Query: 61  VSVPIGSPPQQMDMVVDTGSQLSWIQCHGKVRRKSVKPMINWFDPYLSSSFSFLPCNTTL 120
           VSVPIGSPPQQMDMVVDTGSQLSWIQCHGKVRRKSVKPMINWFDPYLSSSFSFLPCNTTL
Sbjct: 61  VSVPIGSPPQQMDMVVDTGSQLSWIQCHGKVRRKSVKPMINWFDPYLSSSFSFLPCNTTL 120

Query: 121 CRPRIPDFTLPTSCDPTRHCHYSYFYADGTLAEGNLVTEKFTFSNSLTTRSLLLGCATAS 180
           CRPRIPDFTLPTSCDPTRHCHYSYFYADGTLAEGNLVTEKFTFSNSLTTRSLLLGCATAS
Sbjct: 121 CRPRIPDFTLPTSCDPTRHCHYSYFYADGTLAEGNLVTEKFTFSNSLTTRSLLLGCATAS 180

Query: 181 TQNRGMLGMNTGRLSFISQAKISKFSYCVPDRTGSDLTGLFYLGDNPNSAKFKYVNMLTF 240
           TQNRGMLGMNTGRLSFISQAKISKFSYCVPDRTGSDLTGLFYLGDNPNSAKFKYVNMLTF
Sbjct: 181 TQNRGMLGMNTGRLSFISQAKISKFSYCVPDRTGSDLTGLFYLGDNPNSAKFKYVNMLTF 240

Query: 241 PKSRLSPNLDKSAYTLPMKGIRIGNNKLNISPAVFKPDPSGAGQTMIDSGSDLTYLVDEA 300
           PKSRLSPNLDKSAYTLPMKGIRIGNNKLNISPAVFKPDPSGAGQTMIDSGSDLTYLVDEA
Sbjct: 241 PKSRLSPNLDKSAYTLPMKGIRIGNNKLNISPAVFKPDPSGAGQTMIDSGSDLTYLVDEA 300

Query: 301 YSKVREEMVRLVGPMMKKGYEYAAVADMCFDGAVAAAVGRRIGDMWFQFENGVEILVGKG 360
           YSKVREEMVRLVGPMMKKGYEYAAVADMCFDGAVAAAVGRRIGDMWFQFENGVEILVGKG
Sbjct: 301 YSKVREEMVRLVGPMMKKGYEYAAVADMCFDGAVAAAVGRRIGDMWFQFENGVEILVGKG 360

Query: 361 EGLLTEVEEGVKCVGIGRSDRLVTESNIIGNVHQQNMWVEYDLSNKRIGFGVAKCSGLKA 420
           EGLLTEVEEGVKCVGIGRSDRLVTESNIIGNVHQQNMWVEYDLSNKRIGFGVAKCSGLKA
Sbjct: 361 EGLLTEVEEGVKCVGIGRSDRLVTESNIIGNVHQQNMWVEYDLSNKRIGFGVAKCSGLKA 420

BLAST of CmoCh01G009790 vs. NCBI nr
Match: KAG6607573.1 (Aspartic proteinase PCS1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 837.4 bits (2162), Expect = 5.4e-239
Identity = 418/420 (99.52%), Postives = 419/420 (99.76%), Query Frame = 0

Query: 1   MPLSLLLLSLLGLLFSPSNSLSLSFPLTSHSVSSQEASLSLSSKTKSHGKFPFQYSNALV 60
           MPLSLLLLSLLGLLFSPSNSLSLSFPLT HSVSSQEASLSLSSKTKSHGKFPFQYSNALV
Sbjct: 1   MPLSLLLLSLLGLLFSPSNSLSLSFPLTYHSVSSQEASLSLSSKTKSHGKFPFQYSNALV 60

Query: 61  VSVPIGSPPQQMDMVVDTGSQLSWIQCHGKVRRKSVKPMINWFDPYLSSSFSFLPCNTTL 120
           VSVPIGSPPQQMDMVVDTGSQLSWIQCHGKVRRKSVKPMINWFDPYLSSSFSFLPCNTTL
Sbjct: 61  VSVPIGSPPQQMDMVVDTGSQLSWIQCHGKVRRKSVKPMINWFDPYLSSSFSFLPCNTTL 120

Query: 121 CRPRIPDFTLPTSCDPTRHCHYSYFYADGTLAEGNLVTEKFTFSNSLTTRSLLLGCATAS 180
           CRPRIPDFTLPTSCDPTRHCHYSYFYADGTLAEGNLVTEKFTFSNSLTTRSLLLGCATAS
Sbjct: 121 CRPRIPDFTLPTSCDPTRHCHYSYFYADGTLAEGNLVTEKFTFSNSLTTRSLLLGCATAS 180

Query: 181 TQNRGMLGMNTGRLSFISQAKISKFSYCVPDRTGSDLTGLFYLGDNPNSAKFKYVNMLTF 240
           TQNRGMLGMNTGRLSFISQAKISKFSYCVPDRTGSDLTGLFYLGDNPNSAKFKYVNMLTF
Sbjct: 181 TQNRGMLGMNTGRLSFISQAKISKFSYCVPDRTGSDLTGLFYLGDNPNSAKFKYVNMLTF 240

Query: 241 PKSRLSPNLDKSAYTLPMKGIRIGNNKLNISPAVFKPDPSGAGQTMIDSGSDLTYLVDEA 300
           PKSRLSPNLDKSAYTLPMKGIRIGNNKLNISPAVFKPDPSGAGQTMIDSGSDLTYLVDEA
Sbjct: 241 PKSRLSPNLDKSAYTLPMKGIRIGNNKLNISPAVFKPDPSGAGQTMIDSGSDLTYLVDEA 300

Query: 301 YSKVREEMVRLVGPMMKKGYEYAAVADMCFDGAVAAAVGRRIGDMWFQFENGVEILVGKG 360
           YSKVREEMVRLVGPMMKKGYEYAAVADMCFDGAVAAAVGRRIGDMWFQFENGVEILVGKG
Sbjct: 301 YSKVREEMVRLVGPMMKKGYEYAAVADMCFDGAVAAAVGRRIGDMWFQFENGVEILVGKG 360

Query: 361 EGLLTEVEEGVKCVGIGRSDRLVTESNIIGNVHQQNMWVEYDLSNKRIGFGVAKCSGLKA 420
           EGLLTEVEEGVKCVGIGRSDRLVTESNIIGNVHQQNMWVEYDLSNKR+GFGVAKCSGLKA
Sbjct: 361 EGLLTEVEEGVKCVGIGRSDRLVTESNIIGNVHQQNMWVEYDLSNKRVGFGVAKCSGLKA 420

BLAST of CmoCh01G009790 vs. TAIR 10
Match: AT5G37540.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 485.3 bits (1248), Expect = 4.8e-137
Identity = 258/433 (59.58%), Postives = 313/433 (72.29%), Query Frame = 0

Query: 2   PLSLLLLSLL-----GLLFSPSNSLSLSFPLTSHSVSSQEASLS-----LSSKTKSHGKF 61
           PL L LL +       +  S S+SLSL FPLTS  ++    S S     LS +  S    
Sbjct: 7   PLFLKLLYIFFFFCYSVSLSWSSSLSLHFPLTSLRLTPTTNSSSFKTSLLSRRNPSPPSS 66

Query: 62  PF------QYSNALVVSVPIGSPPQQMDMVVDTGSQLSWIQCHGKVRRKSVKPMINWFDP 121
           P+      +YS AL++S+PIG+P Q  ++V+DTGSQLSWIQCH K  +K + P    FDP
Sbjct: 67  PYTFRSNIKYSMALILSLPIGTPSQSQELVLDTGSQLSWIQCHPKKIKKPLPPPTTSFDP 126

Query: 122 YLSSSFSFLPCNTTLCRPRIPDFTLPTSCDPTRHCHYSYFYADGTLAEGNLVTEKFTFSN 181
            LSSSFS LPC+  LC+PRIPDFTLPTSCD  R CHYSYFYADGT AEGNLV EKFTFSN
Sbjct: 127 SLSSSFSDLPCSHPLCKPRIPDFTLPTSCDSNRLCHYSYFYADGTFAEGNLVKEKFTFSN 186

Query: 182 SLTTRSLLLGCATASTQNRGMLGMNTGRLSFISQAKISKFSYCVP---DRTGSDLTGLFY 241
           S TT  L+LGCA  ST  +G+LGMN GRLSFISQAKISKFSYC+P   +R G   TG FY
Sbjct: 187 SQTTPPLILGCAKESTDEKGILGMNLGRLSFISQAKISKFSYCIPTRSNRPGLASTGSFY 246

Query: 242 LGDNPNSAKFKYVNMLTFPKSRLSPNLDKSAYTLPMKGIRIGNNKLNISPAVFKPDPSGA 301
           LGDNPNS  FKYV++LTFP+S+  PNLD  AYT+P++GIRIG  +LNI  +VF+PD  G+
Sbjct: 247 LGDNPNSRGFKYVSLLTFPQSQRMPNLDPLAYTVPLQGIRIGQKRLNIPGSVFRPDAGGS 306

Query: 302 GQTMIDSGSDLTYLVDEAYSKVREEMVRLVGPMMKKGYEYAAVADMCFDGAVAAAVGRRI 361
           GQTM+DSGS+ T+LVD AY KV+EE+VRLVG  +KKGY Y + ADMCFDG  +  +GR I
Sbjct: 307 GQTMVDSGSEFTHLVDVAYDKVKEEIVRLVGSRLKKGYVYGSTADMCFDGNHSMEIGRLI 366

Query: 362 GDMWFQFENGVEILVGKGEGLLTEVEEGVKCVGIGRSDRLVTESNIIGNVHQQNMWVEYD 416
           GD+ F+F  GVEILV K + LL  V  G+ CVGIGRS  L   SNIIGNVHQQN+WVE+D
Sbjct: 367 GDLVFEFGRGVEILVEK-QSLLVNVGGGIHCVGIGRSSMLGAASNIIGNVHQQNLWVEFD 426

BLAST of CmoCh01G009790 vs. TAIR 10
Match: AT1G66180.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 470.7 bits (1210), Expect = 1.2e-132
Identity = 253/415 (60.96%), Postives = 298/415 (71.81%), Query Frame = 0

Query: 16  SPSNSLSLSFPLTSHSVS----SQEASLSLSSKTKSHGKFP-------FQYSNALVVSVP 75
           S S SLSL  PLTS  +S    S   + SL S+       P       F+YS AL++S+P
Sbjct: 18  SLSTSLSLHLPLTSLPISTTTNSHRFTTSLLSRKNPSPSSPPYNFRSRFKYSMALIISLP 77

Query: 76  IGSPPQQMDMVVDTGSQLSWIQCHGKVRRKSVKPMINWFDPYLSSSFSFLPCNTTLCRPR 135
           IG+PPQ   MV+DTGSQLSWIQCH K  +   KP  + FDP LSSSFS LPC+  LC+PR
Sbjct: 78  IGTPPQAQQMVLDTGSQLSWIQCHRK--KLPPKPKTS-FDPSLSSSFSTLPCSHPLCKPR 137

Query: 136 IPDFTLPTSCDPTRHCHYSYFYADGTLAEGNLVTEKFTFSNSLTTRSLLLGCATASTQNR 195
           IPDFTLPTSCD  R CHYSYFYADGT AEGNLV EK TFSN+  T  L+LGCAT S+ +R
Sbjct: 138 IPDFTLPTSCDSNRLCHYSYFYADGTFAEGNLVKEKITFSNTEITPPLILGCATESSDDR 197

Query: 196 GMLGMNTGRLSFISQAKISKFSYCVP---DRTGSDLTGLFYLGDNPNSAKFKYVNMLTFP 255
           G+LGMN GRLSF+SQAKISKFSYC+P   +R G   TG FYLGDNPNS  FKYV++LTFP
Sbjct: 198 GILGMNRGRLSFVSQAKISKFSYCIPPKSNRPGFTPTGSFYLGDNPNSHGFKYVSLLTFP 257

Query: 256 KSRLSPNLDKSAYTLPMKGIRIGNNKLNISPAVFKPDPSGAGQTMIDSGSDLTYLVDEAY 315
           +S+  PNLD  AYT+PM GIR G  KLNIS +VF+PD  G+GQTM+DSGS+ T+LVD AY
Sbjct: 258 ESQRMPNLDPLAYTVPMIGIRFGLKKLNISGSVFRPDAGGSGQTMVDSGSEFTHLVDAAY 317

Query: 316 SKVREEMVRLVGPMMKKGYEYAAVADMCFDGAVAAAVGRRIGDMWFQFENGVEILVGKGE 375
            KVR E++  VG  +KKGY Y   ADMCFDG V A + R IGD+ F F  GVEILV K E
Sbjct: 318 DKVRAEIMTRVGRRLKKGYVYGGTADMCFDGNV-AMIPRLIGDLVFVFTRGVEILVPK-E 377

Query: 376 GLLTEVEEGVKCVGIGRSDRLVTESNIIGNVHQQNMWVEYDLSNKRIGFGVAKCS 417
            +L  V  G+ CVGIGRS  L   SNIIGNVHQQN+WVE+D++N+R+GF  A CS
Sbjct: 378 RVLVNVGGGIHCVGIGRSSMLGAASNIIGNVHQQNLWVEFDVTNRRVGFAKADCS 427

BLAST of CmoCh01G009790 vs. TAIR 10
Match: AT5G02190.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 248.8 bits (634), Expect = 7.6e-66
Identity = 157/432 (36.34%), Postives = 228/432 (52.78%), Query Frame = 0

Query: 16  SPSNSLSLSFPLTSHSVSSQEASLSLSSKT-------KSHGKFPFQYSNALVVSVPIGSP 75
           S S+S S SF  +S S SS   +L L  KT       +   K  F ++  L V++ +G+P
Sbjct: 23  SSSSSSSSSFSFSSFSSSSSSQTLVLPLKTRITPTDHRPTDKLHFHHNVTLTVTLTVGTP 82

Query: 76  PQQMDMVVDTGSQLSWIQCHGKVRRKSVKPMINWFDPYLSSSFSFLPCNTTLCRPRIPDF 135
           PQ + MV+DTGS+LSW++C+    R S    +N FDP  SSS+S +PC++  CR R  DF
Sbjct: 83  PQNISMVIDTGSELSWLRCN----RSSNPNPVNNFDPTRSSSYSPIPCSSPTCRTRTRDF 142

Query: 136 TLPTSCDPTRHCHYSYFYADGTLAEGNLVTEKFTFSNSLTTRSLLLGCATA--------S 195
            +P SCD  + CH +  YAD + +EGNL  E F F NS    +L+ GC  +         
Sbjct: 143 LIPASCDSDKLCHATLSYADASSSEGNLAAEIFHFGNSTNDSNLIFGCMGSVSGSDPEED 202

Query: 196 TQNRGMLGMNTGRLSFISQAKISKFSYCVPDRTGSDLTGLFYLGDNPNSAKFKYVNMLTF 255
           T+  G+LGMN G LSFISQ    KFSYC+      D  G   LGD    + F ++  L +
Sbjct: 203 TKTTGLLGMNRGSLSFISQMGFPKFSYCISGT--DDFPGFLLLGD----SNFTWLTPLNY 262

Query: 256 -PKSRLS---PNLDKSAYTLPMKGIRIGNNKLNISPAVFKPDPSGAGQTMIDSGSDLTYL 315
            P  R+S   P  D+ AYT+ + GI++    L I  +V  PD +GAGQTM+DSG+  T+L
Sbjct: 263 TPLIRISTPLPYFDRVAYTVQLTGIKVNGKLLPIPKSVLVPDHTGAGQTMVDSGTQFTFL 322

Query: 316 VDEAYSKVREEMVRLVGPMM----KKGYEYAAVADMCF---DGAVAAAVGRRIGDMWFQF 375
           +   Y+ +R   +     ++       + +    D+C+      + + +  R+  +   F
Sbjct: 323 LGPVYTALRSHFLNRTNGILTVYEDPDFVFQGTMDLCYRISPVRIRSGILHRLPTVSLVF 382

Query: 376 ENGVEILVGKGEGLLTEV------EEGVKCVGIGRSDRLVTESNIIGNVHQQNMWVEYDL 416
           E G EI V  G+ LL  V       + V C   G SD +  E+ +IG+ HQQNMW+E+DL
Sbjct: 383 E-GAEIAV-SGQPLLYRVPHLTVGNDSVYCFTFGNSDLMGMEAYVIGHHHQQNMWIEFDL 442

BLAST of CmoCh01G009790 vs. TAIR 10
Match: AT2G39710.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 239.2 bits (609), Expect = 6.0e-63
Identity = 156/442 (35.29%), Postives = 233/442 (52.71%), Query Frame = 0

Query: 16  SPSNSLSLS------------FPLTSHSVSSQEASLSLSSKTK-----SHGKFPFQYSNA 75
           S S+SLSLS            FPLT    SS   +L  S KT+     S  K  F+++  
Sbjct: 5   SSSSSLSLSKNFLRISVLLLIFPLTFCKTSSTNQTLLFSLKTQKLPQSSSDKLSFRHNVT 64

Query: 76  LVVSVPIGSPPQQMDMVVDTGSQLSWIQCHGKVRRKSVKPMINWFDPYLSSSFSFLPCNT 135
           L V++ +G PPQ + MV+DTGS+LSW+ C       SV      F+P  SS++S +PC++
Sbjct: 65  LTVTLAVGDPPQNISMVLDTGSELSWLHCKKSPNLGSV------FNPVSSSTYSPVPCSS 124

Query: 136 TLCRPRIPDFTLPTSCDPTRH-CHYSYFYADGTLAEGNLVTEKFTFSNSLTTRSLLLGC- 195
            +CR R  D  +P SCDP  H CH +  YAD T  EGNL  E F    S+T    L GC 
Sbjct: 125 PICRTRTRDLPIPASCDPKTHLCHVAISYADATSIEGNLAHETFVI-GSVTRPGTLFGCM 184

Query: 196 -------ATASTQNRGMLGMNTGRLSFISQAKISKFSYCVPDRTGSDLTGLFYLGDNPNS 255
                  +    ++ G++GMN G LSF++Q   SKFSYC+   +GSD +G   LGD    
Sbjct: 185 DSGLSSNSEEDAKSTGLMGMNRGSLSFVNQLGFSKFSYCI---SGSDSSGFLLLGD---- 244

Query: 256 AKFKYVNMLTFP----KSRLSPNLDKSAYTLPMKGIRIGNNKLNISPAVFKPDPSGAGQT 315
           A + ++  + +     +S   P  D+ AYT+ ++GIR+G+  L++  +VF PD +GAGQT
Sbjct: 245 ASYSWLGPIQYTPLVLQSTPLPYFDRVAYTVQLEGIRVGSKILSLPKSVFVPDHTGAGQT 304

Query: 316 MIDSGSDLTYLVDEAYSKVREEMVRLVGPMMK----KGYEYAAVADMCFDGAVAAAVGRR 375
           M+DSG+  T+L+   Y+ ++ E +     +++      + +    D+C+           
Sbjct: 305 MVDSGTQFTFLMGPVYTALKNEFITQTKSVLRLVDDPDFVFQGTMDLCYKVGSTTRPNFS 364

Query: 376 IGDMWFQFENGVEILVGKGEGLL-------TEVEEGVKCVGIGRSDRLVTESNIIGNVHQ 416
              M      G E+ V  G+ LL       +E +E V C   G SD L  E+ +IG+ HQ
Sbjct: 365 GLPMVSLMFRGAEMSV-SGQKLLYRVNGAGSEGKEEVYCFTFGNSDLLGIEAFVIGHHHQ 424

BLAST of CmoCh01G009790 vs. TAIR 10
Match: AT2G03200.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 150.2 bits (378), Expect = 3.7e-36
Identity = 118/390 (30.26%), Postives = 184/390 (47.18%), Query Frame = 0

Query: 44  KTKSHGKFPFQYSNALVVSVPIGSPPQQMDMVVDTGSQLSWIQCHGKVRRKSVKPMINWF 103
           K  +HG      S   ++ + IG+P  +   +VDTGS L W QC  K   +        F
Sbjct: 97  KAPTHGG-----SGEFLMELSIGNPAVKYSAIVDTGSDLIWTQC--KPCTECFDQPTPIF 156

Query: 104 DPYLSSSFSFLPCNTTLCRPRIPDFTLPTS-CDPTRH-CHYSYFYADGTLAEGNLVTEKF 163
           DP  SSS+S + C++ LC        LP S C+  +  C Y Y Y D +   G L TE F
Sbjct: 157 DPEKSSSYSKVGCSSGLCN------ALPRSNCNEDKDACEYLYTYGDYSSTRGLLATETF 216

Query: 164 TFSNSLTTRSLLLGCATAS-----TQNRGMLGMNTGRLSFISQAKISKFSYCVPDRTGSD 223
           TF +  +   +  GC   +     +Q  G++G+  G LS ISQ K +KFSYC+     S+
Sbjct: 217 TFEDENSISGIGFGCGVENEGDGFSQGSGLVGLGRGPLSLISQLKETKFSYCLTSIEDSE 276

Query: 224 LTGLFYLGDNP----NSAKFKYVNMLTFPKSRLSPNLDKSAYTLPMKGIRIGNNKLNISP 283
            +   ++G       N         +T   S L      S Y L ++GI +G  +L++  
Sbjct: 277 ASSSLFIGSLASGIVNKTGASLDGEVTKTMSLLRNPDQPSFYYLELQGITVGAKRLSVEK 336

Query: 284 AVFKPDPSGAGQTMIDSGSDLTYLVDEAYSKVREEMV-RLVGPMMKKGYEYAAVADMCF- 343
           + F+    G G  +IDSG+ +TYL + A+  ++EE   R+  P+   G   +   D+CF 
Sbjct: 337 STFELAEDGTGGMIIDSGTTITYLEETAFKVLKEEFTSRMSLPVDDSG---STGLDLCFK 396

Query: 344 --DGAVAAAVGRRIGDMWFQFENGVEILVGKGEGLLTEVEEGVKCVGIGRSDRLVTESNI 403
             D A   AV + I    F F+ G ++ +     ++ +   GV C+ +G S+ +    +I
Sbjct: 397 LPDAAKNIAVPKMI----FHFK-GADLELPGENYMVADSSTGVLCLAMGSSNGM----SI 456

Query: 404 IGNVHQQNMWVEYDLSNKRIGFGVAKCSGL 419
            GNV QQN  V +DL  + + F   +C  L
Sbjct: 457 FGNVQQQNFNVLHDLEKETVSFVPTECGKL 461

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9LZL31.1e-6436.34Aspartic proteinase PCS1 OS=Arabidopsis thaliana OX=3702 GN=PCS1 PE=2 SV=1[more]
Q766C32.1e-3630.39Aspartic proteinase nepenthesin-1 OS=Nepenthes gracilis OX=150966 GN=nep1 PE=1 S... [more]
Q766C23.0e-3530.68Aspartic proteinase nepenthesin-2 OS=Nepenthes gracilis OX=150966 GN=nep2 PE=1 S... [more]
Q9LS409.8e-3429.41Protein ASPARTIC PROTEASE IN GUARD CELL 1 OS=Arabidopsis thaliana OX=3702 GN=ASP... [more]
Q9LTW41.7e-3028.34Aspartic proteinase NANA, chloroplast OS=Arabidopsis thaliana OX=3702 GN=NANA PE... [more]
Match NameE-valueIdentityDescription
A0A6J1EWZ64.0e-240100.00aspartic proteinase PCS1-like OS=Cucurbita moschata OX=3662 GN=LOC111438863 PE=3... [more]
A0A6J1F0M75.2e-24099.76aspartic proteinase PCS1-like OS=Cucurbita moschata OX=3662 GN=LOC111438404 PE=3... [more]
A0A6J1F1811.2e-23999.76aspartic proteinase PCS1-like OS=Cucurbita moschata OX=3662 GN=LOC111438406 PE=3... [more]
A0A6J1F1P32.0e-23999.76aspartic proteinase PCS1-like OS=Cucurbita moschata OX=3662 GN=LOC111438856 PE=3... [more]
A0A6J1I6122.5e-23497.62aspartic proteinase PCS1-like OS=Cucurbita maxima OX=3661 GN=LOC111471392 PE=3 S... [more]
Match NameE-valueIdentityDescription
XP_022932084.18.3e-240100.00aspartic proteinase PCS1-like [Cucurbita moschata] >XP_022932086.1 aspartic prot... [more]
XP_022932083.11.1e-23999.76aspartic proteinase PCS1-like [Cucurbita moschata][more]
XP_022932085.12.4e-23999.76aspartic proteinase PCS1-like [Cucurbita moschata][more]
XP_022932433.14.1e-23999.76aspartic proteinase PCS1-like [Cucurbita moschata][more]
KAG6607573.15.4e-23999.52Aspartic proteinase PCS1, partial [Cucurbita argyrosperma subsp. sororia][more]
Match NameE-valueIdentityDescription
AT5G37540.14.8e-13759.58Eukaryotic aspartyl protease family protein [more]
AT1G66180.11.2e-13260.96Eukaryotic aspartyl protease family protein [more]
AT5G02190.17.6e-6636.34Eukaryotic aspartyl protease family protein [more]
AT2G39710.16.0e-6335.29Eukaryotic aspartyl protease family protein [more]
AT2G03200.13.7e-3630.26Eukaryotic aspartyl protease family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001461Aspartic peptidase A1 familyPRINTSPR00792PEPSINcoord: 285..296
score: 28.66
coord: 387..402
score: 25.24
coord: 65..85
score: 43.55
IPR001461Aspartic peptidase A1 familyPANTHERPTHR47965ASPARTYL PROTEASE-RELATEDcoord: 26..416
IPR021109Aspartic peptidase domain superfamilyGENE3D2.40.70.10Acid Proteasescoord: 225..419
e-value: 1.2E-38
score: 134.5
IPR021109Aspartic peptidase domain superfamilyGENE3D2.40.70.10Acid Proteasescoord: 33..224
e-value: 1.1E-32
score: 115.5
IPR021109Aspartic peptidase domain superfamilySUPERFAMILY50630Acid proteasescoord: 60..416
IPR032861Xylanase inhibitor, N-terminalPFAMPF14543TAXi_Ncoord: 60..225
e-value: 2.6E-34
score: 119.0
IPR032799Xylanase inhibitor, C-terminalPFAMPF14541TAXi_Ccoord: 253..410
e-value: 1.0E-30
score: 106.7
NoneNo IPR availablePANTHERPTHR47965:SF51EUKARYOTIC ASPARTYL PROTEASE FAMILY PROTEINcoord: 26..416
IPR001969Aspartic peptidase, active sitePROSITEPS00141ASP_PROTEASEcoord: 74..85
IPR033121Peptidase family A1 domainPROSITEPS51767PEPTIDASE_A1coord: 59..411
score: 31.361834
IPR034161Pepsin-like domain, plantCDDcd05476pepsin_A_like_plantcoord: 60..415
e-value: 1.56036E-71
score: 224.064

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh01G009790.1CmoCh01G009790.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
molecular_function GO:0004190 aspartic-type endopeptidase activity