Homology
BLAST of CmoCh01G009010 vs. ExPASy Swiss-Prot
Match:
Q69ZF3 (Non-lysosomal glucosylceramidase OS=Mus musculus OX=10090 GN=Gba2 PE=1 SV=2)
HSP 1 Score: 579.3 bits (1492), Expect = 7.8e-164
Identity = 326/863 (37.78%), Postives = 460/863 (53.30%), Query Frame = 0
Query: 52 HLG-PLGYRLWRHGKEEAAKGRIPLYDFFASPPITCYHGVSLGGIGAGSIGRSYRGEFQR 111
HLG L Y W + K K + P D S P+ +G LGGIG G+I R +RG+F R
Sbjct: 105 HLGMGLRYLKWWYRKTHVEK-KTPFIDMLNSLPLRQIYGCPLGGIGGGTITRGWRGQFCR 164
Query: 112 FQMFYGPSEDVPVLANQFSAFVSRPNGNKFSTVLCSARPQKSKDGKNDGIEAWDWNLSGE 171
+Q+ G + V+A+QF + R + VL P + + +W+W L G
Sbjct: 165 WQLNPGMYQHQTVIADQFIVCLRRDGRTVYQQVLSLELP--------NVLRSWNWGLCGY 224
Query: 172 KSTYHALFPRAWTIYDGEPDPDLKIVCRQISPIIPHNYKESSFPVSVFTFNLSNKGPTSA 231
+ YHAL+PRAWT+Y P ++ + CRQ++PI+PH+Y++SS PV VF +++ N+G +
Sbjct: 225 FAFYHALYPRAWTVYQ-LPGQNVTLTCRQVTPILPHDYQDSSLPVGVFVWDVENEGDETL 284
Query: 232 EVTLLFTWANSVGGNSGFTGHHFNSKIGAEDG---AHGVLLHHKTANGRPTVTYAITAEA 291
+V++ F+ N +GG G +N E G G+LLHH T T A+ A
Sbjct: 285 DVSITFSMRNGLGGEDDAAGSLWNEPFRLEQGGTTVQGLLLHHPTPPN--PYTMAVAARC 344
Query: 292 TDDVHVSVCPRFVISGDSEGISAKDMWQEVKKHGSFDELGSSGPHEGSKPGSSIGAAIAA 351
T D V+ F +G + + +WQ++ + G D +G ++ G I A+
Sbjct: 345 TADTTVTHTTAFDPNG-----TGQQVWQDLLQDGQLD--SPAGQSTPTQKGEGIAGAVCV 404
Query: 352 KVSIPSSSTRTVTFSLAWDCPEVKFDGKT--YHRRYTNFYGTLGEAAKTIARDAILEHGK 411
+ S + FSLAWD P++ F K+ ++RRYT F+G+ G+ A ++ A+ +
Sbjct: 405 SSKLLPRSRCCLEFSLAWDMPKIMFGAKSQVHYRRYTRFFGSDGDVAPALSHYALCHYAD 464
Query: 412 WEGQIEAWQRPIVEDKRLPEWYPVTLFNELYFLNSGGTMWTDGLPPLQNLSTISHQKIFL 471
WE +I AWQ P+++D+ LP WY LFNELYFL GGT+W
Sbjct: 465 WEDRISAWQNPVLDDRTLPAWYKSALFNELYFLADGGTVW-------------------- 524
Query: 472 ERTKSEPNGAPNGDHRLDVAVEILERMSQIFDQAHGGAGPSNAALGTRLLPTLQENVGQL 531
L+V + L P R L + ++ G+
Sbjct: 525 ----------------LEVPADSL---------------PEGLGGSMRQLRSTLQDYGRF 584
Query: 532 LLVEGSEYLMWNTFDVHFYSSFALIMLFPKLELGIQRDFAAAVLMHDPRKAKIMSDGKYY 591
+EG EY M+NT+DVHFY+SFAL+ML+PKLEL +Q D A A L D + + + G
Sbjct: 585 GYLEGQEYRMYNTYDVHFYASFALVMLWPKLELSLQYDMALATLKEDLTRRRYLMSGVVA 644
Query: 592 PRKVIGAVPHDIGFND--PWFEVNAYNLLNVTRWKDLGSKFVLQVYRDVVATGDKNFAKS 651
P K +PHDIG D PW VNAY + + WKDL KFVLQ+YRD TGD+ F +
Sbjct: 645 PVKRRNVIPHDIGDPDDEPWLRVNAYLIHDTADWKDLNLKFVLQIYRDYYLTGDQGFLED 704
Query: 652 VWPSVYVALAFMDQFDKDKDGMIENEGFPDQTYDVWTVKGVSAYCGGLWVAALQAASALA 711
+WP + +FDKD+DG+IEN G+ DQTYD W G SAYCGGLW+AA+ +A
Sbjct: 705 MWPVCLAVMESEMKFDKDQDGLIENGGYADQTYDAWVTTGPSAYCGGLWLAAVAVMVQMA 764
Query: 712 YEVDDEAAAHYFWIKYQKARGVYD-TLWNGSYFNYDNSGGPWSSSIQADQLAGQWYARAC 771
+ F + R Y+ LWNG Y+NYD+S P S SI +DQ AGQW+ RAC
Sbjct: 765 VLCGAQDVQERFASILCRGREAYERLLWNGRYYNYDSSSHPQSRSIMSDQCAGQWFLRAC 824
Query: 772 GL----CPIVDEEKIRSTLEKIFNFNVMKVKGGTRGAVNGMFPDGRVDRSILQPKEIWAG 831
GL + + L+ IF NV GG GAVNGM P G DRS +Q E+W G
Sbjct: 825 GLGEGDTEVFPTLHVVRALQTIFELNVQAFAGGAMGAVNGMHPHGVPDRSSVQSDEVWVG 884
Query: 832 VTYSVAASMIQEGMVETGFQTAMGIHQAAWGQDGLGYSFQTPEAWDIDDKYRSLGYMRPL 891
V Y +AA+MIQEG+ GF+TA G ++ W + LG +FQTPEA+ +RSL YMRPL
Sbjct: 885 VVYGLAATMIQEGLTWEGFRTAEGCYRTVW--ERLGLAFQTPEAYCQQQVFRSLAYMRPL 891
Query: 892 AIWAMQWAISKPTLLKKHKVPRK 902
+IWAMQ A+ + ++HK R+
Sbjct: 945 SIWAMQLALQQ----QQHKKSRR 891
BLAST of CmoCh01G009010 vs. ExPASy Swiss-Prot
Match:
Q9HCG7 (Non-lysosomal glucosylceramidase OS=Homo sapiens OX=9606 GN=GBA2 PE=1 SV=2)
HSP 1 Score: 575.1 bits (1481), Expect = 1.5e-162
Identity = 330/853 (38.69%), Postives = 461/853 (54.04%), Query Frame = 0
Query: 52 HLG-PLGYRLWRHGKEEAAKGRIPLYDFFASPPITCYHGVSLGGIGAGSIGRSYRGEFQR 111
H+G L Y W + K K + P D S P+ +G LGGIG G+I R +RG+F R
Sbjct: 114 HIGMGLRYLQWWYRKTHVEK-KTPFIDMINSVPLRQIYGCPLGGIGGGTITRGWRGQFCR 173
Query: 112 FQMFYGPSEDVPVLANQFSAFVSRPNGNKFSTVLCSARPQKSKDGKNDGIEAWDWNLSGE 171
+Q+ G + V+A+QF+ + R + VL RP + +W+W L G
Sbjct: 174 WQLNPGMYQHRTVIADQFTVCLRREGQTVYQQVLSLERPSV--------LRSWNWGLCGY 233
Query: 172 KSTYHALFPRAWTIYDGEPDPDLKIVCRQISPIIPHNYKESSFPVSVFTFNLSNKGPTSA 231
+ YHAL+PRAWT+Y P ++ + CRQI+PI+PH+Y++SS PV VF +++ N+G +
Sbjct: 234 FAFYHALYPRAWTVYQ-LPGQNVTLTCRQITPILPHDYQDSSLPVGVFVWDVENEGDEAL 293
Query: 232 EVTLLFTWANSVGGNSGFTGHHFNSKIGAE---DGAHGVLLHHKTANGRPTVTYA--ITA 291
+V+++F+ N +GG G +N E + G+LLHH T T+ A +TA
Sbjct: 294 DVSIMFSMRNGLGGGDDAPGGLWNEPFCLERSGETVRGLLLHHPTLPNPYTMAVAARVTA 353
Query: 292 EATDDVHVSVCPRFVISGDSEGISAKDMWQEVKKHGSFDELGSSGPHEGSKPGSSIGAAI 351
AT H++ DS G + +WQ++ + G D +G ++ G I A+
Sbjct: 354 -ATTVTHIT-----AFDPDSTG---QQVWQDLLQDGQLD--SPTGQSTPTQKGVGIAGAV 413
Query: 352 AAKVSIPSSSTRTVTFSLAWDCPEVKF--DGKTYHRRYTNFYGTLGEAAKTIARDAILEH 411
+ + FSLAWD P + F G+ ++RRYT F+G G+AA ++ A+ +
Sbjct: 414 CVSSKLRPRGQCRLEFSLAWDMPRIMFGAKGQVHYRRYTRFFGQDGDAAPALSHYALCRY 473
Query: 412 GKWEGQIEAWQRPIVEDKRLPEWYPVTLFNELYFLNSGGTMWTDGLPPLQNLSTISHQKI 471
+WE +I AWQ P+++D+ LP WY LFNELYFL GGT+W + L
Sbjct: 474 AEWEERISAWQSPVLDDRSLPAWYKSALFNELYFLADGGTVWLEVLE------------- 533
Query: 472 FLERTKSEPNGAPNGDHRLDVAVEILERMSQIFDQAHGGAGPSNAALGTRLLPTLQENVG 531
D E L R + H L PTL++ G
Sbjct: 534 -------------------DSLPEELGR-----NMCH-------------LRPTLRD-YG 593
Query: 532 QLLLVEGSEYLMWNTFDVHFYSSFALIMLFPKLELGIQRDFAAAVLMHDPRKAKIMSDGK 591
+ +EG EY M+NT+DVHFY+SFALIML+PKLEL +Q D A A L D + + + G
Sbjct: 594 RFGYLEGQEYRMYNTYDVHFYASFALIMLWPKLELSLQYDMALATLREDLTRRRYLMSGV 653
Query: 592 YYPRKVIGAVPHDIGFND--PWFEVNAYNLLNVTRWKDLGSKFVLQVYRDVVATGDKNFA 651
P K +PHDIG D PW VNAY + + WKDL KFVLQVYRD TGD+NF
Sbjct: 654 MAPVKRRNVIPHDIGDPDDEPWLRVNAYLIHDTADWKDLNLKFVLQVYRDYYLTGDQNFL 713
Query: 652 KSVWPSVYVALAFMDQFDKDKDGMIENEGFPDQTYDVWTVKGVSAYCGGLWVAALQAASA 711
K +WP + +FDKD DG+IEN G+ DQTYD W G SAYCGGLW+AA+
Sbjct: 714 KDMWPVCLAVMESEMKFDKDHDGLIENGGYADQTYDGWVTTGPSAYCGGLWLAAVAVMVQ 773
Query: 712 LAYEVDDEAAAHYFWIKYQKARGVYD-TLWNGSYFNYDNSGGPWSSSIQADQLAGQWYAR 771
+A + F + + Y+ LWNG Y+NYD+S P S S+ +DQ AGQW+ +
Sbjct: 774 MAALCGAQDIQDKFSSILSRGQEAYERLLWNGRYYNYDSSSRPQSRSVMSDQCAGQWFLK 833
Query: 772 ACGL----CPIVDEEKIRSTLEKIFNFNVMKVKGGTRGAVNGMFPDGRVDRSILQPKEIW 831
ACGL + + + L+ IF NV GG GAVNGM P G D+S +Q E+W
Sbjct: 834 ACGLGEGDTEVFPTQHVVRALQTIFELNVQAFAGGAMGAVNGMQPHGVPDKSSVQSDEVW 892
Query: 832 AGVTYSVAASMIQEGMVETGFQTAMGIHQAAWGQDGLGYSFQTPEAWDIDDKYRSLGYMR 890
GV Y +AA+MIQEG+ GFQTA G ++ W + LG +FQTPEA+ +RSL YMR
Sbjct: 894 VGVVYGLAATMIQEGLTWEGFQTAEGCYRTVW--ERLGLAFQTPEAYCQQRVFRSLAYMR 892
BLAST of CmoCh01G009010 vs. ExPASy Swiss-Prot
Match:
Q5M868 (Non-lysosomal glucosylceramidase OS=Rattus norvegicus OX=10116 GN=Gba2 PE=2 SV=2)
HSP 1 Score: 573.2 bits (1476), Expect = 5.6e-162
Identity = 328/856 (38.32%), Postives = 455/856 (53.15%), Query Frame = 0
Query: 56 LGYRLWRHGKEEAAKGRIPLYDFFASPPITCYHGVSLGGIGAGSIGRSYRGEFQRFQMFY 115
L Y W + K + K + P D F S P+ +G LGGIG G+I R +RG+F R+Q+
Sbjct: 110 LRYLKWWYRKTQVEK-KTPFIDMFNSVPLRQIYGCPLGGIGGGTITRGWRGQFCRWQLNP 169
Query: 116 GPSEDVPVLANQFSAFVSRPNGNKFSTVLCSARPQKSKDGKNDGIEAWDWNLSGEKSTYH 175
G + V+A+QF + R + VL P + +W+W L G + YH
Sbjct: 170 GMYQHQTVIADQFIVCLRRDGRTVYQQVLSLELPSV--------LRSWNWGLCGYFAFYH 229
Query: 176 ALFPRAWTIYDGEPDPDLKIVCRQISPIIPHNYKESSFPVSVFTFNLSNKGPTSAEVTLL 235
AL+PRAWT+Y P ++ + CRQI+PI+PH+Y++SS PV VF +++ N+G + +V+++
Sbjct: 230 ALYPRAWTVYQ-LPGQNVTLTCRQITPILPHDYQDSSLPVGVFVWDVENEGDETLDVSIM 289
Query: 236 FTWANSVGGNSGFTGHHFNSKIGAE-DG--AHGVLLHHKTANGRPTVTYAITAEATDDVH 295
F+ N +GG G +N E DG G+LLHH T T A+ A T D
Sbjct: 290 FSMRNGLGGEDDAAGGLWNEPFRLEQDGTTVQGLLLHHPTPPN--PYTMAVAARHTADTT 349
Query: 296 VSVCPRFVISGDSEGISAKDMWQEVKKHGSFDELGSSGPHEGSKPGSSIGAAIAAKVSIP 355
V+ F DS G + +WQ++ + G D +G ++ G + A+ A +
Sbjct: 350 VTYTTAF--DPDSTG---QQVWQDLLQDGQLD--SPAGQSTPTQRGEGVAGAVCASSKLL 409
Query: 356 SSSTRTVTFSLAWDCPEVKF--DGKTYHRRYTNFYGTLGEAAKTIARDAILEHGKWEGQI 415
+ FSLAWD P + F G+ ++RRYT F+G+ G+ A ++ A+ ++ WE I
Sbjct: 410 PRGRCCLEFSLAWDMPRIMFGAKGQVHYRRYTRFFGSDGDVAPALSHYALCQYAGWENSI 469
Query: 416 EAWQRPIVEDKRLPEWYPVTLFNELYFLNSGGTMWTDGLPPLQNLSTISHQKIFLERTKS 475
AWQ P+++D+ LP WY LFNELYFL GGT+W
Sbjct: 470 SAWQNPVLDDRSLPAWYKSALFNELYFLADGGTVW------------------------- 529
Query: 476 EPNGAPNGDHRLDVAVEILERMSQIFDQAHGGAGPSNAALGTRLLPTLQENVGQLLLVEG 535
LE + GG+ +L P LQ+ G+ +EG
Sbjct: 530 ------------------LEVPEDSLPEELGGS-------MYQLRPILQD-YGRFGYLEG 589
Query: 536 SEYLMWNTFDVHFYSSFALIMLFPKLELGIQRDFAAAVLMHDPRKAKIMSDGKYYPRKVI 595
EY M+NT+DVHFY+SFAL+ML+PKLEL +Q D A A D + + + G P K
Sbjct: 590 QEYRMYNTYDVHFYASFALVMLWPKLELSLQYDMALATFKEDLTRRRYLMSGVVAPVKRR 649
Query: 596 GAVPHDIGFND--PWFEVNAYNLLNVTRWKDLGSKFVLQVYRDVVATGDKNFAKSVWPSV 655
+PHDIG D PW VNAY + + WKDL KFVLQVYRD TGD+ F K +WP
Sbjct: 650 NVIPHDIGDPDDEPWLRVNAYLIHDTADWKDLNLKFVLQVYRDYYLTGDQGFLKDMWPVC 709
Query: 656 YVALAFMDQFDKDKDGMIENEGFPDQTYDVWTVKGVSAYCGGLWVAALQAASALAYEVDD 715
+ +FDKD+DG+IEN G+ DQTYD W G SAYCGGLW+AA+ +A
Sbjct: 710 LAVMESEMKFDKDQDGLIENGGYADQTYDGWVTTGPSAYCGGLWLAAVAVMVQMAVLCGA 769
Query: 716 EAAAHYFWIKYQKARGVYD-TLWNGSYFNYDNSGGPWSSSIQADQLAGQWYARACGL--- 775
+ F + R Y+ LWNG Y+NYD+S P S S+ +DQ AGQW+ RACGL
Sbjct: 770 QDVQDKFSSILCRGREAYERLLWNGRYYNYDSSSQPQSRSVMSDQCAGQWFLRACGLGEG 829
Query: 776 -CPIVDEEKIRSTLEKIFNFNVMKVKGGTRGAVNGMFPDGRVDRSILQPKEIWAGVTYSV 835
+ + L+ IF NV GG GAVNGM P G DRS +Q E+W GV Y +
Sbjct: 830 DTEVFPTLHVVRALKTIFELNVQAFAGGAMGAVNGMQPHGVPDRSSVQSDEVWVGVVYGL 889
Query: 836 AASMIQEGMVETGFQTAMGIHQAAWGQDGLGYSFQTPEAWDIDDKYRSLGYMRPLAIWAM 895
AA+MIQEG+ GF+TA G ++ W + LG +FQTPEA+ +RSL YMRPL+IWAM
Sbjct: 890 AATMIQEGLTWEGFRTAEGCYRTVW--ERLGLAFQTPEAYCQQRVFRSLAYMRPLSIWAM 893
Query: 896 QWAISKPTLLKKHKVP 900
Q A+ + K P
Sbjct: 950 QLALQQQQHKKNSSRP 893
BLAST of CmoCh01G009010 vs. ExPASy Swiss-Prot
Match:
Q7KT91 (Non-lysosomal glucosylceramidase OS=Drosophila melanogaster OX=7227 GN=CG33090 PE=1 SV=1)
HSP 1 Score: 490.0 bits (1260), Expect = 6.2e-137
Identity = 313/932 (33.58%), Postives = 463/932 (49.68%), Query Frame = 0
Query: 55 PLGYRLWRHGKEEAAKGRIPLYDFFASPPITCYHGVSLGGIGAGSIGRSYRGEFQRFQMF 114
PL R + + + +GR D++ +GV +GGIG G+IGR Y GEF RFQM
Sbjct: 83 PLVCRYAAYYWKVSREGRRVYMDYYYMENGKQIYGVPIGGIGGGTIGRGYAGEFCRFQMR 142
Query: 115 YGPSEDVPVLANQFSAFVSRPNG-NKFSTVL--CSARPQKS-KDGKNDG----------- 174
G E VLANQF + P G F ++L CS R + S DG DG
Sbjct: 143 PGIYEYNVVLANQFIVTIKDPKGCTIFQSLLSKCSTRDKTSDPDGDPDGERTKCQLPNCS 202
Query: 175 ------IEAWDWNLSGEKSTYHALFPRAWTIYDGEPDPDLKIVCRQISPIIPHNYKESSF 234
+ AW N+ + +Y L+PR+WT YD +++ CRQ+SP+IPH Y+ESS
Sbjct: 203 SRAKQPLSAWHSNIEDTRCSYTGLYPRSWTEYD-LSHYGVRLTCRQVSPVIPHEYRESSL 262
Query: 235 PVSVFTFNLSNKGPTSAEVTLLFTWANSVGGNSGFTGHHFNSKIGAEDGAHGVLLHHKTA 294
P +VF +++ N +V++ FT+ N G S++ +E A GV + K +
Sbjct: 263 PCAVFVWSVENVCDQERKVSITFTFKNGTGNKKQDAEGGAESQLISEGNAKGVSIRQKIS 322
Query: 295 NGRPTVTYAITAEATDDVHVSVCPRFVISGDSEGISAKDMWQEVKKHGSFDELGSSGPHE 354
+Y + ++ ++ CP+F +G+ E +W ++K+HG E P
Sbjct: 323 --EMPCSYNLACRVLPEISITRCPQFDPAGNGE-----QLWAQLKEHGQLSE----HPTS 382
Query: 355 GSKPGSSIGAAIAAKVSIPSSSTRTVTFSLAWDCPEVKFDGK--TYHRRYTNFYGTLGEA 414
+ IG A+ +V++ ++ + F LAWD P+++F K T+ R YT ++ G++
Sbjct: 383 EALKTKDIGVAVCGQVALKPMASHDLEFVLAWDMPKIQFPRKMQTHTRYYTKYFDDSGDS 442
Query: 415 AKTIARDAILEHGKWEGQIEAWQRPIVEDKRLPEWYPVTLFNELYFLNSGGTMWTDGLPP 474
I A+ ++ WE I+AWQRPI+ D+ LP+WY +FN+LYF++ GGT+W
Sbjct: 443 GPRICEYALRQYSTWERLIDAWQRPILNDETLPDWYKCAIFNQLYFISDGGTIW------ 502
Query: 475 LQNLSTISHQKIFLERTKSEPNGAPNGDHRLDVAVEILERMSQIFDQAHGGAGPSNAALG 534
L+ S++ + + D RL A+G G
Sbjct: 503 LKCDSSLGKELAY-------------DDPRL----------------AYGRFG------- 562
Query: 535 TRLLPTLQENVGQLLLVEGSEYLMWNTFDVHFYSSFALIMLFPKLELGIQRDFAAAVLMH 594
+EG EY M+NT+DVHFY+S AL L+P L++ +Q DF A+
Sbjct: 563 ---------------YLEGHEYRMYNTYDVHFYASPALAHLWPNLQVSLQYDFKDAIAAE 622
Query: 595 DPRKAKIMSDGKYYPRKVIGAVPHDIGFND--PWFEVNAYNLLNVTRWKDLGSKFVLQVY 654
K++ DGK PRKV VPHD+G D P+ +N YN+ +V WKDL +KFVLQVY
Sbjct: 623 LNDTRKMLYDGKVMPRKVKNCVPHDLGDPDEEPFTLINCYNIHDVNDWKDLNTKFVLQVY 682
Query: 655 RDVVATGDKNFAKSVWPSVYVALAFMD--------------------------------- 714
RD + A+S S + ++ F+D
Sbjct: 683 RDYYVLNELAQAQSDNASKFSSIEFIDKESLYELYSQDNKRKNSADEKQQNRKSASMYIN 742
Query: 715 ------------------------------QFDKDKDGMIENEGFPDQTYDVWTVKGVSA 774
++DKD DG+IEN PDQTYD W + G SA
Sbjct: 743 ETNGKVYLMDAIGYLKAMYASCKAIMERTIEYDKDNDGLIENTKMPDQTYDSWVMDGPSA 802
Query: 775 YCGGLWVAALQAASALAYEVDD-EAAAHYFWIKYQKARGVYDTLWNGSYFNYDNSGGPWS 834
YC GLW+AALQA SA+A +D Y I + R + + LWNGSY+ +D S
Sbjct: 803 YCSGLWLAALQAMSAMATILDQPNDCLRYQDILEKGKRSLEEKLWNGSYYRFDLSHS-HR 862
Query: 835 SSIQADQLAGQWYARACGL-CPIVDEEKIRSTLEKIFNFNVMKVKGGTRGAVNGMFPD-- 890
+I ADQL G WY ++CG I +E +R+ L++I++ NVM G GA NG +
Sbjct: 863 DTIMADQLCGHWYLKSCGFDYEIYPKENVRTALKRIYDNNVMGFHEGNIGAANGFIANAS 922
BLAST of CmoCh01G009010 vs. ExPASy TrEMBL
Match:
A0A6J1GAM9 (Non-lysosomal glucosylceramidase OS=Cucurbita moschata OX=3662 GN=LOC111452445 PE=3 SV=1)
HSP 1 Score: 1976.1 bits (5118), Expect = 0.0e+00
Identity = 951/951 (100.00%), Postives = 951/951 (100.00%), Query Frame = 0
Query: 1 MENGCKEDADNGASSTMVVDPAKLPSLTWQRKLDFTGKSPESFSFTLNDKWHLGPLGYRL 60
MENGCKEDADNGASSTMVVDPAKLPSLTWQRKLDFTGKSPESFSFTLNDKWHLGPLGYRL
Sbjct: 1 MENGCKEDADNGASSTMVVDPAKLPSLTWQRKLDFTGKSPESFSFTLNDKWHLGPLGYRL 60
Query: 61 WRHGKEEAAKGRIPLYDFFASPPITCYHGVSLGGIGAGSIGRSYRGEFQRFQMFYGPSED 120
WRHGKEEAAKGRIPLYDFFASPPITCYHGVSLGGIGAGSIGRSYRGEFQRFQMFYGPSED
Sbjct: 61 WRHGKEEAAKGRIPLYDFFASPPITCYHGVSLGGIGAGSIGRSYRGEFQRFQMFYGPSED 120
Query: 121 VPVLANQFSAFVSRPNGNKFSTVLCSARPQKSKDGKNDGIEAWDWNLSGEKSTYHALFPR 180
VPVLANQFSAFVSRPNGNKFSTVLCSARPQKSKDGKNDGIEAWDWNLSGEKSTYHALFPR
Sbjct: 121 VPVLANQFSAFVSRPNGNKFSTVLCSARPQKSKDGKNDGIEAWDWNLSGEKSTYHALFPR 180
Query: 181 AWTIYDGEPDPDLKIVCRQISPIIPHNYKESSFPVSVFTFNLSNKGPTSAEVTLLFTWAN 240
AWTIYDGEPDPDLKIVCRQISPIIPHNYKESSFPVSVFTFNLSNKGPTSAEVTLLFTWAN
Sbjct: 181 AWTIYDGEPDPDLKIVCRQISPIIPHNYKESSFPVSVFTFNLSNKGPTSAEVTLLFTWAN 240
Query: 241 SVGGNSGFTGHHFNSKIGAEDGAHGVLLHHKTANGRPTVTYAITAEATDDVHVSVCPRFV 300
SVGGNSGFTGHHFNSKIGAEDGAHGVLLHHKTANGRPTVTYAITAEATDDVHVSVCPRFV
Sbjct: 241 SVGGNSGFTGHHFNSKIGAEDGAHGVLLHHKTANGRPTVTYAITAEATDDVHVSVCPRFV 300
Query: 301 ISGDSEGISAKDMWQEVKKHGSFDELGSSGPHEGSKPGSSIGAAIAAKVSIPSSSTRTVT 360
ISGDSEGISAKDMWQEVKKHGSFDELGSSGPHEGSKPGSSIGAAIAAKVSIPSSSTRTVT
Sbjct: 301 ISGDSEGISAKDMWQEVKKHGSFDELGSSGPHEGSKPGSSIGAAIAAKVSIPSSSTRTVT 360
Query: 361 FSLAWDCPEVKFDGKTYHRRYTNFYGTLGEAAKTIARDAILEHGKWEGQIEAWQRPIVED 420
FSLAWDCPEVKFDGKTYHRRYTNFYGTLGEAAKTIARDAILEHGKWEGQIEAWQRPIVED
Sbjct: 361 FSLAWDCPEVKFDGKTYHRRYTNFYGTLGEAAKTIARDAILEHGKWEGQIEAWQRPIVED 420
Query: 421 KRLPEWYPVTLFNELYFLNSGGTMWTDGLPPLQNLSTISHQKIFLERTKSEPNGAPNGDH 480
KRLPEWYPVTLFNELYFLNSGGTMWTDGLPPLQNLSTISHQKIFLERTKSEPNGAPNGDH
Sbjct: 421 KRLPEWYPVTLFNELYFLNSGGTMWTDGLPPLQNLSTISHQKIFLERTKSEPNGAPNGDH 480
Query: 481 RLDVAVEILERMSQIFDQAHGGAGPSNAALGTRLLPTLQENVGQLLLVEGSEYLMWNTFD 540
RLDVAVEILERMSQIFDQAHGGAGPSNAALGTRLLPTLQENVGQLLLVEGSEYLMWNTFD
Sbjct: 481 RLDVAVEILERMSQIFDQAHGGAGPSNAALGTRLLPTLQENVGQLLLVEGSEYLMWNTFD 540
Query: 541 VHFYSSFALIMLFPKLELGIQRDFAAAVLMHDPRKAKIMSDGKYYPRKVIGAVPHDIGFN 600
VHFYSSFALIMLFPKLELGIQRDFAAAVLMHDPRKAKIMSDGKYYPRKVIGAVPHDIGFN
Sbjct: 541 VHFYSSFALIMLFPKLELGIQRDFAAAVLMHDPRKAKIMSDGKYYPRKVIGAVPHDIGFN 600
Query: 601 DPWFEVNAYNLLNVTRWKDLGSKFVLQVYRDVVATGDKNFAKSVWPSVYVALAFMDQFDK 660
DPWFEVNAYNLLNVTRWKDLGSKFVLQVYRDVVATGDKNFAKSVWPSVYVALAFMDQFDK
Sbjct: 601 DPWFEVNAYNLLNVTRWKDLGSKFVLQVYRDVVATGDKNFAKSVWPSVYVALAFMDQFDK 660
Query: 661 DKDGMIENEGFPDQTYDVWTVKGVSAYCGGLWVAALQAASALAYEVDDEAAAHYFWIKYQ 720
DKDGMIENEGFPDQTYDVWTVKGVSAYCGGLWVAALQAASALAYEVDDEAAAHYFWIKYQ
Sbjct: 661 DKDGMIENEGFPDQTYDVWTVKGVSAYCGGLWVAALQAASALAYEVDDEAAAHYFWIKYQ 720
Query: 721 KARGVYDTLWNGSYFNYDNSGGPWSSSIQADQLAGQWYARACGLCPIVDEEKIRSTLEKI 780
KARGVYDTLWNGSYFNYDNSGGPWSSSIQADQLAGQWYARACGLCPIVDEEKIRSTLEKI
Sbjct: 721 KARGVYDTLWNGSYFNYDNSGGPWSSSIQADQLAGQWYARACGLCPIVDEEKIRSTLEKI 780
Query: 781 FNFNVMKVKGGTRGAVNGMFPDGRVDRSILQPKEIWAGVTYSVAASMIQEGMVETGFQTA 840
FNFNVMKVKGGTRGAVNGMFPDGRVDRSILQPKEIWAGVTYSVAASMIQEGMVETGFQTA
Sbjct: 781 FNFNVMKVKGGTRGAVNGMFPDGRVDRSILQPKEIWAGVTYSVAASMIQEGMVETGFQTA 840
Query: 841 MGIHQAAWGQDGLGYSFQTPEAWDIDDKYRSLGYMRPLAIWAMQWAISKPTLLKKHKVPR 900
MGIHQAAWGQDGLGYSFQTPEAWDIDDKYRSLGYMRPLAIWAMQWAISKPTLLKKHKVPR
Sbjct: 841 MGIHQAAWGQDGLGYSFQTPEAWDIDDKYRSLGYMRPLAIWAMQWAISKPTLLKKHKVPR 900
Query: 901 KELSESEESCLFSTQHAAFLKVASLLKLPSEEAKPRSVVEVAYDFICKRSA 952
KELSESEESCLFSTQHAAFLKVASLLKLPSEEAKPRSVVEVAYDFICKRSA
Sbjct: 901 KELSESEESCLFSTQHAAFLKVASLLKLPSEEAKPRSVVEVAYDFICKRSA 951
BLAST of CmoCh01G009010 vs. ExPASy TrEMBL
Match:
A0A6J1GBB8 (Non-lysosomal glucosylceramidase OS=Cucurbita moschata OX=3662 GN=LOC111452445 PE=3 SV=1)
HSP 1 Score: 1968.7 bits (5099), Expect = 0.0e+00
Identity = 951/959 (99.17%), Postives = 951/959 (99.17%), Query Frame = 0
Query: 1 MENGCKEDADNGASSTMVVDPAKLPSLTWQRKLDFTGKSPESFSFTLNDKWHLGPLGYRL 60
MENGCKEDADNGASSTMVVDPAKLPSLTWQRKLDFTGKSPESFSFTLNDKWHLGPLGYRL
Sbjct: 1 MENGCKEDADNGASSTMVVDPAKLPSLTWQRKLDFTGKSPESFSFTLNDKWHLGPLGYRL 60
Query: 61 WRHGKEEAAKGRIPLYDFFASPPITCYHGVSLGGIGAGSIGRSYRGEFQRFQMFYGPSED 120
WRHGKEEAAKGRIPLYDFFASPPITCYHGVSLGGIGAGSIGRSYRGEFQRFQMFYGPSED
Sbjct: 61 WRHGKEEAAKGRIPLYDFFASPPITCYHGVSLGGIGAGSIGRSYRGEFQRFQMFYGPSED 120
Query: 121 VPVLANQFSAFVSRPNGNKFSTVLCSARPQKSKDGKNDGIEAWDWNLSGEKSTYHALFPR 180
VPVLANQFSAFVSRPNGNKFSTVLCSARPQKSKDGKNDGIEAWDWNLSGEKSTYHALFPR
Sbjct: 121 VPVLANQFSAFVSRPNGNKFSTVLCSARPQKSKDGKNDGIEAWDWNLSGEKSTYHALFPR 180
Query: 181 AWTIYDGEPDPDLKIVCRQISPIIPHNYKESSFPVSVFTFNLSNKGPTSAEVTLLFTWAN 240
AWTIYDGEPDPDLKIVCRQISPIIPHNYKESSFPVSVFTFNLSNKGPTSAEVTLLFTWAN
Sbjct: 181 AWTIYDGEPDPDLKIVCRQISPIIPHNYKESSFPVSVFTFNLSNKGPTSAEVTLLFTWAN 240
Query: 241 SVGGNSGFTGHHFNSKIG--------AEDGAHGVLLHHKTANGRPTVTYAITAEATDDVH 300
SVGGNSGFTGHHFNSKIG AEDGAHGVLLHHKTANGRPTVTYAITAEATDDVH
Sbjct: 241 SVGGNSGFTGHHFNSKIGVKAEFVSSAEDGAHGVLLHHKTANGRPTVTYAITAEATDDVH 300
Query: 301 VSVCPRFVISGDSEGISAKDMWQEVKKHGSFDELGSSGPHEGSKPGSSIGAAIAAKVSIP 360
VSVCPRFVISGDSEGISAKDMWQEVKKHGSFDELGSSGPHEGSKPGSSIGAAIAAKVSIP
Sbjct: 301 VSVCPRFVISGDSEGISAKDMWQEVKKHGSFDELGSSGPHEGSKPGSSIGAAIAAKVSIP 360
Query: 361 SSSTRTVTFSLAWDCPEVKFDGKTYHRRYTNFYGTLGEAAKTIARDAILEHGKWEGQIEA 420
SSSTRTVTFSLAWDCPEVKFDGKTYHRRYTNFYGTLGEAAKTIARDAILEHGKWEGQIEA
Sbjct: 361 SSSTRTVTFSLAWDCPEVKFDGKTYHRRYTNFYGTLGEAAKTIARDAILEHGKWEGQIEA 420
Query: 421 WQRPIVEDKRLPEWYPVTLFNELYFLNSGGTMWTDGLPPLQNLSTISHQKIFLERTKSEP 480
WQRPIVEDKRLPEWYPVTLFNELYFLNSGGTMWTDGLPPLQNLSTISHQKIFLERTKSEP
Sbjct: 421 WQRPIVEDKRLPEWYPVTLFNELYFLNSGGTMWTDGLPPLQNLSTISHQKIFLERTKSEP 480
Query: 481 NGAPNGDHRLDVAVEILERMSQIFDQAHGGAGPSNAALGTRLLPTLQENVGQLLLVEGSE 540
NGAPNGDHRLDVAVEILERMSQIFDQAHGGAGPSNAALGTRLLPTLQENVGQLLLVEGSE
Sbjct: 481 NGAPNGDHRLDVAVEILERMSQIFDQAHGGAGPSNAALGTRLLPTLQENVGQLLLVEGSE 540
Query: 541 YLMWNTFDVHFYSSFALIMLFPKLELGIQRDFAAAVLMHDPRKAKIMSDGKYYPRKVIGA 600
YLMWNTFDVHFYSSFALIMLFPKLELGIQRDFAAAVLMHDPRKAKIMSDGKYYPRKVIGA
Sbjct: 541 YLMWNTFDVHFYSSFALIMLFPKLELGIQRDFAAAVLMHDPRKAKIMSDGKYYPRKVIGA 600
Query: 601 VPHDIGFNDPWFEVNAYNLLNVTRWKDLGSKFVLQVYRDVVATGDKNFAKSVWPSVYVAL 660
VPHDIGFNDPWFEVNAYNLLNVTRWKDLGSKFVLQVYRDVVATGDKNFAKSVWPSVYVAL
Sbjct: 601 VPHDIGFNDPWFEVNAYNLLNVTRWKDLGSKFVLQVYRDVVATGDKNFAKSVWPSVYVAL 660
Query: 661 AFMDQFDKDKDGMIENEGFPDQTYDVWTVKGVSAYCGGLWVAALQAASALAYEVDDEAAA 720
AFMDQFDKDKDGMIENEGFPDQTYDVWTVKGVSAYCGGLWVAALQAASALAYEVDDEAAA
Sbjct: 661 AFMDQFDKDKDGMIENEGFPDQTYDVWTVKGVSAYCGGLWVAALQAASALAYEVDDEAAA 720
Query: 721 HYFWIKYQKARGVYDTLWNGSYFNYDNSGGPWSSSIQADQLAGQWYARACGLCPIVDEEK 780
HYFWIKYQKARGVYDTLWNGSYFNYDNSGGPWSSSIQADQLAGQWYARACGLCPIVDEEK
Sbjct: 721 HYFWIKYQKARGVYDTLWNGSYFNYDNSGGPWSSSIQADQLAGQWYARACGLCPIVDEEK 780
Query: 781 IRSTLEKIFNFNVMKVKGGTRGAVNGMFPDGRVDRSILQPKEIWAGVTYSVAASMIQEGM 840
IRSTLEKIFNFNVMKVKGGTRGAVNGMFPDGRVDRSILQPKEIWAGVTYSVAASMIQEGM
Sbjct: 781 IRSTLEKIFNFNVMKVKGGTRGAVNGMFPDGRVDRSILQPKEIWAGVTYSVAASMIQEGM 840
Query: 841 VETGFQTAMGIHQAAWGQDGLGYSFQTPEAWDIDDKYRSLGYMRPLAIWAMQWAISKPTL 900
VETGFQTAMGIHQAAWGQDGLGYSFQTPEAWDIDDKYRSLGYMRPLAIWAMQWAISKPTL
Sbjct: 841 VETGFQTAMGIHQAAWGQDGLGYSFQTPEAWDIDDKYRSLGYMRPLAIWAMQWAISKPTL 900
Query: 901 LKKHKVPRKELSESEESCLFSTQHAAFLKVASLLKLPSEEAKPRSVVEVAYDFICKRSA 952
LKKHKVPRKELSESEESCLFSTQHAAFLKVASLLKLPSEEAKPRSVVEVAYDFICKRSA
Sbjct: 901 LKKHKVPRKELSESEESCLFSTQHAAFLKVASLLKLPSEEAKPRSVVEVAYDFICKRSA 959
BLAST of CmoCh01G009010 vs. ExPASy TrEMBL
Match:
A0A6J1GAL9 (Non-lysosomal glucosylceramidase OS=Cucurbita moschata OX=3662 GN=LOC111452445 PE=3 SV=1)
HSP 1 Score: 1962.2 bits (5082), Expect = 0.0e+00
Identity = 950/959 (99.06%), Postives = 950/959 (99.06%), Query Frame = 0
Query: 1 MENGCKEDADNGASSTMVVDPAKLPSLTWQRKLDFTGKSPESFSFTLNDKWHLGPLGYRL 60
MENGCKEDADNGASSTM VDPAKLPSLTWQRKLDFTGKSPESFSFTLNDKWHLGPLGYRL
Sbjct: 1 MENGCKEDADNGASSTM-VDPAKLPSLTWQRKLDFTGKSPESFSFTLNDKWHLGPLGYRL 60
Query: 61 WRHGKEEAAKGRIPLYDFFASPPITCYHGVSLGGIGAGSIGRSYRGEFQRFQMFYGPSED 120
WRHGKEEAAKGRIPLYDFFASPPITCYHGVSLGGIGAGSIGRSYRGEFQRFQMFYGPSED
Sbjct: 61 WRHGKEEAAKGRIPLYDFFASPPITCYHGVSLGGIGAGSIGRSYRGEFQRFQMFYGPSED 120
Query: 121 VPVLANQFSAFVSRPNGNKFSTVLCSARPQKSKDGKNDGIEAWDWNLSGEKSTYHALFPR 180
VPVLANQFSAFVSRPNGNKFSTVLCSARPQKSKDGKNDGIEAWDWNLSGEKSTYHALFPR
Sbjct: 121 VPVLANQFSAFVSRPNGNKFSTVLCSARPQKSKDGKNDGIEAWDWNLSGEKSTYHALFPR 180
Query: 181 AWTIYDGEPDPDLKIVCRQISPIIPHNYKESSFPVSVFTFNLSNKGPTSAEVTLLFTWAN 240
AWTIYDGEPDPDLKIVCRQISPIIPHNYKESSFPVSVFTFNLSNKGPTSAEVTLLFTWAN
Sbjct: 181 AWTIYDGEPDPDLKIVCRQISPIIPHNYKESSFPVSVFTFNLSNKGPTSAEVTLLFTWAN 240
Query: 241 SVGGNSGFTGHHFNSKIG--------AEDGAHGVLLHHKTANGRPTVTYAITAEATDDVH 300
SVGGNSGFTGHHFNSKIG AEDGAHGVLLHHKTANGRPTVTYAITAEATDDVH
Sbjct: 241 SVGGNSGFTGHHFNSKIGVKAEFVSSAEDGAHGVLLHHKTANGRPTVTYAITAEATDDVH 300
Query: 301 VSVCPRFVISGDSEGISAKDMWQEVKKHGSFDELGSSGPHEGSKPGSSIGAAIAAKVSIP 360
VSVCPRFVISGDSEGISAKDMWQEVKKHGSFDELGSSGPHEGSKPGSSIGAAIAAKVSIP
Sbjct: 301 VSVCPRFVISGDSEGISAKDMWQEVKKHGSFDELGSSGPHEGSKPGSSIGAAIAAKVSIP 360
Query: 361 SSSTRTVTFSLAWDCPEVKFDGKTYHRRYTNFYGTLGEAAKTIARDAILEHGKWEGQIEA 420
SSSTRTVTFSLAWDCPEVKFDGKTYHRRYTNFYGTLGEAAKTIARDAILEHGKWEGQIEA
Sbjct: 361 SSSTRTVTFSLAWDCPEVKFDGKTYHRRYTNFYGTLGEAAKTIARDAILEHGKWEGQIEA 420
Query: 421 WQRPIVEDKRLPEWYPVTLFNELYFLNSGGTMWTDGLPPLQNLSTISHQKIFLERTKSEP 480
WQRPIVEDKRLPEWYPVTLFNELYFLNSGGTMWTDGLPPLQNLSTISHQKIFLERTKSEP
Sbjct: 421 WQRPIVEDKRLPEWYPVTLFNELYFLNSGGTMWTDGLPPLQNLSTISHQKIFLERTKSEP 480
Query: 481 NGAPNGDHRLDVAVEILERMSQIFDQAHGGAGPSNAALGTRLLPTLQENVGQLLLVEGSE 540
NGAPNGDHRLDVAVEILERMSQIFDQAHGGAGPSNAALGTRLLPTLQENVGQLLLVEGSE
Sbjct: 481 NGAPNGDHRLDVAVEILERMSQIFDQAHGGAGPSNAALGTRLLPTLQENVGQLLLVEGSE 540
Query: 541 YLMWNTFDVHFYSSFALIMLFPKLELGIQRDFAAAVLMHDPRKAKIMSDGKYYPRKVIGA 600
YLMWNTFDVHFYSSFALIMLFPKLELGIQRDFAAAVLMHDPRKAKIMSDGKYYPRKVIGA
Sbjct: 541 YLMWNTFDVHFYSSFALIMLFPKLELGIQRDFAAAVLMHDPRKAKIMSDGKYYPRKVIGA 600
Query: 601 VPHDIGFNDPWFEVNAYNLLNVTRWKDLGSKFVLQVYRDVVATGDKNFAKSVWPSVYVAL 660
VPHDIGFNDPWFEVNAYNLLNVTRWKDLGSKFVLQVYRDVVATGDKNFAKSVWPSVYVAL
Sbjct: 601 VPHDIGFNDPWFEVNAYNLLNVTRWKDLGSKFVLQVYRDVVATGDKNFAKSVWPSVYVAL 660
Query: 661 AFMDQFDKDKDGMIENEGFPDQTYDVWTVKGVSAYCGGLWVAALQAASALAYEVDDEAAA 720
AFMDQFDKDKDGMIENEGFPDQTYDVWTVKGVSAYCGGLWVAALQAASALAYEVDDEAAA
Sbjct: 661 AFMDQFDKDKDGMIENEGFPDQTYDVWTVKGVSAYCGGLWVAALQAASALAYEVDDEAAA 720
Query: 721 HYFWIKYQKARGVYDTLWNGSYFNYDNSGGPWSSSIQADQLAGQWYARACGLCPIVDEEK 780
HYFWIKYQKARGVYDTLWNGSYFNYDNSGGPWSSSIQADQLAGQWYARACGLCPIVDEEK
Sbjct: 721 HYFWIKYQKARGVYDTLWNGSYFNYDNSGGPWSSSIQADQLAGQWYARACGLCPIVDEEK 780
Query: 781 IRSTLEKIFNFNVMKVKGGTRGAVNGMFPDGRVDRSILQPKEIWAGVTYSVAASMIQEGM 840
IRSTLEKIFNFNVMKVKGGTRGAVNGMFPDGRVDRSILQPKEIWAGVTYSVAASMIQEGM
Sbjct: 781 IRSTLEKIFNFNVMKVKGGTRGAVNGMFPDGRVDRSILQPKEIWAGVTYSVAASMIQEGM 840
Query: 841 VETGFQTAMGIHQAAWGQDGLGYSFQTPEAWDIDDKYRSLGYMRPLAIWAMQWAISKPTL 900
VETGFQTAMGIHQAAWGQDGLGYSFQTPEAWDIDDKYRSLGYMRPLAIWAMQWAISKPTL
Sbjct: 841 VETGFQTAMGIHQAAWGQDGLGYSFQTPEAWDIDDKYRSLGYMRPLAIWAMQWAISKPTL 900
Query: 901 LKKHKVPRKELSESEESCLFSTQHAAFLKVASLLKLPSEEAKPRSVVEVAYDFICKRSA 952
LKKHKVPRKELSESEESCLFSTQHAAFLKVASLLKLPSEEAKPRSVVEVAYDFICKRSA
Sbjct: 901 LKKHKVPRKELSESEESCLFSTQHAAFLKVASLLKLPSEEAKPRSVVEVAYDFICKRSA 958
BLAST of CmoCh01G009010 vs. ExPASy TrEMBL
Match:
A0A6J1I8G5 (Non-lysosomal glucosylceramidase OS=Cucurbita maxima OX=3661 GN=LOC111471931 PE=3 SV=1)
HSP 1 Score: 1949.5 bits (5049), Expect = 0.0e+00
Identity = 936/951 (98.42%), Postives = 943/951 (99.16%), Query Frame = 0
Query: 1 MENGCKEDADNGASSTMVVDPAKLPSLTWQRKLDFTGKSPESFSFTLNDKWHLGPLGYRL 60
M+NGCKE+ADNG SSTMVVDPAKLPSLTWQRKLDFTGKSPESFSFTLNDKWHLGPLGYRL
Sbjct: 1 MKNGCKEEADNGDSSTMVVDPAKLPSLTWQRKLDFTGKSPESFSFTLNDKWHLGPLGYRL 60
Query: 61 WRHGKEEAAKGRIPLYDFFASPPITCYHGVSLGGIGAGSIGRSYRGEFQRFQMFYGPSED 120
WRHGKEEAAKGRIPLYDFFASPPITCYHGVSLGGIGAGSIGRSYRGEFQRFQMFYGPSED
Sbjct: 61 WRHGKEEAAKGRIPLYDFFASPPITCYHGVSLGGIGAGSIGRSYRGEFQRFQMFYGPSED 120
Query: 121 VPVLANQFSAFVSRPNGNKFSTVLCSARPQKSKDGKNDGIEAWDWNLSGEKSTYHALFPR 180
VPVLANQFSAFVSRPNGN+FSTVLCSARPQKSKDGKNDGIEAWDWNLSGEKSTYHALFPR
Sbjct: 121 VPVLANQFSAFVSRPNGNRFSTVLCSARPQKSKDGKNDGIEAWDWNLSGEKSTYHALFPR 180
Query: 181 AWTIYDGEPDPDLKIVCRQISPIIPHNYKESSFPVSVFTFNLSNKGPTSAEVTLLFTWAN 240
AWTIYDGEPDPDLKIVCRQISPIIPHNYKESSFPVSVFTFNLSNKGPTSAEVTLLFTWAN
Sbjct: 181 AWTIYDGEPDPDLKIVCRQISPIIPHNYKESSFPVSVFTFNLSNKGPTSAEVTLLFTWAN 240
Query: 241 SVGGNSGFTGHHFNSKIGAEDGAHGVLLHHKTANGRPTVTYAITAEATDDVHVSVCPRFV 300
SVGGNSGFTGHHFNSKIGAEDGAHGVLLHHKTANGRPTVTYAITAEATDDVHVSVCPRFV
Sbjct: 241 SVGGNSGFTGHHFNSKIGAEDGAHGVLLHHKTANGRPTVTYAITAEATDDVHVSVCPRFV 300
Query: 301 ISGDSEGISAKDMWQEVKKHGSFDELGSSGPHEGSKPGSSIGAAIAAKVSIPSSSTRTVT 360
ISGDSEGISAKDMWQEVK HGSFD+ G+SGPHE SKPGSSIGAAIAAKVSIPSSST TVT
Sbjct: 301 ISGDSEGISAKDMWQEVKNHGSFDKFGNSGPHEESKPGSSIGAAIAAKVSIPSSSTCTVT 360
Query: 361 FSLAWDCPEVKFDGKTYHRRYTNFYGTLGEAAKTIARDAILEHGKWEGQIEAWQRPIVED 420
FSLAWDCPEVKFDGKTYHRRYTNFYGTLGEAAKTI RDAILEHGKWEGQIEAWQRPIVED
Sbjct: 361 FSLAWDCPEVKFDGKTYHRRYTNFYGTLGEAAKTIGRDAILEHGKWEGQIEAWQRPIVED 420
Query: 421 KRLPEWYPVTLFNELYFLNSGGTMWTDGLPPLQNLSTISHQKIFLERTKSEPNGAPNGDH 480
KRLPEWYPVTLFNELYFLNSGGTMWTDGLPPLQNLSTISHQKIFL+RTKSEPNGAPNGDH
Sbjct: 421 KRLPEWYPVTLFNELYFLNSGGTMWTDGLPPLQNLSTISHQKIFLQRTKSEPNGAPNGDH 480
Query: 481 RLDVAVEILERMSQIFDQAHGGAGPSNAALGTRLLPTLQENVGQLLLVEGSEYLMWNTFD 540
RLDVAVEILERMSQIFDQAHGGAGPSNAALGTRLLPTLQENVGQLLLVEGSEYLMWNTFD
Sbjct: 481 RLDVAVEILERMSQIFDQAHGGAGPSNAALGTRLLPTLQENVGQLLLVEGSEYLMWNTFD 540
Query: 541 VHFYSSFALIMLFPKLELGIQRDFAAAVLMHDPRKAKIMSDGKYYPRKVIGAVPHDIGFN 600
VHFYSSFALIMLFPKLELGIQRDFAAAVLMHDPRKAKIMSDGKYYPRKVIGAVPHDIGFN
Sbjct: 541 VHFYSSFALIMLFPKLELGIQRDFAAAVLMHDPRKAKIMSDGKYYPRKVIGAVPHDIGFN 600
Query: 601 DPWFEVNAYNLLNVTRWKDLGSKFVLQVYRDVVATGDKNFAKSVWPSVYVALAFMDQFDK 660
DPWFEVNAYNLLNVTRWKDLGSKFVLQVYRDVVATGDKNFAKSVWPSVYVALAFMDQFDK
Sbjct: 601 DPWFEVNAYNLLNVTRWKDLGSKFVLQVYRDVVATGDKNFAKSVWPSVYVALAFMDQFDK 660
Query: 661 DKDGMIENEGFPDQTYDVWTVKGVSAYCGGLWVAALQAASALAYEVDDEAAAHYFWIKYQ 720
DKDGMIENEGFPDQTYDVWTVKGVSAYCGGLWVAALQAASALAYEVDDEAAAHYFWIKYQ
Sbjct: 661 DKDGMIENEGFPDQTYDVWTVKGVSAYCGGLWVAALQAASALAYEVDDEAAAHYFWIKYQ 720
Query: 721 KARGVYDTLWNGSYFNYDNSGGPWSSSIQADQLAGQWYARACGLCPIVDEEKIRSTLEKI 780
KARGVYDTLWNGSYFNYDNS GPWSSSIQADQLAGQWYARACGLCPIVDEEKIRSTLEKI
Sbjct: 721 KARGVYDTLWNGSYFNYDNSSGPWSSSIQADQLAGQWYARACGLCPIVDEEKIRSTLEKI 780
Query: 781 FNFNVMKVKGGTRGAVNGMFPDGRVDRSILQPKEIWAGVTYSVAASMIQEGMVETGFQTA 840
FNFNVMKVKGGTRGAVNGMFPDGRVDRSILQPKEIWAGVTYSVAASMIQEGMVETGFQTA
Sbjct: 781 FNFNVMKVKGGTRGAVNGMFPDGRVDRSILQPKEIWAGVTYSVAASMIQEGMVETGFQTA 840
Query: 841 MGIHQAAWGQDGLGYSFQTPEAWDIDDKYRSLGYMRPLAIWAMQWAISKPTLLKKHKVPR 900
MGIHQA WGQDGLGYSFQTPEAWDIDDKYRSLGYMRPLAIWAMQWAISKPTLLKKHKVPR
Sbjct: 841 MGIHQAVWGQDGLGYSFQTPEAWDIDDKYRSLGYMRPLAIWAMQWAISKPTLLKKHKVPR 900
Query: 901 KELSESEESCLFSTQHAAFLKVASLLKLPSEEAKPRSVVEVAYDFICKRSA 952
KELSESEESCLF+TQHAAFLKVASLLKLPSEEAKPRSVVEVAYDFICKRSA
Sbjct: 901 KELSESEESCLFATQHAAFLKVASLLKLPSEEAKPRSVVEVAYDFICKRSA 951
BLAST of CmoCh01G009010 vs. ExPASy TrEMBL
Match:
A0A6J1IED1 (Non-lysosomal glucosylceramidase OS=Cucurbita maxima OX=3661 GN=LOC111471931 PE=3 SV=1)
HSP 1 Score: 1942.2 bits (5030), Expect = 0.0e+00
Identity = 936/959 (97.60%), Postives = 943/959 (98.33%), Query Frame = 0
Query: 1 MENGCKEDADNGASSTMVVDPAKLPSLTWQRKLDFTGKSPESFSFTLNDKWHLGPLGYRL 60
M+NGCKE+ADNG SSTMVVDPAKLPSLTWQRKLDFTGKSPESFSFTLNDKWHLGPLGYRL
Sbjct: 1 MKNGCKEEADNGDSSTMVVDPAKLPSLTWQRKLDFTGKSPESFSFTLNDKWHLGPLGYRL 60
Query: 61 WRHGKEEAAKGRIPLYDFFASPPITCYHGVSLGGIGAGSIGRSYRGEFQRFQMFYGPSED 120
WRHGKEEAAKGRIPLYDFFASPPITCYHGVSLGGIGAGSIGRSYRGEFQRFQMFYGPSED
Sbjct: 61 WRHGKEEAAKGRIPLYDFFASPPITCYHGVSLGGIGAGSIGRSYRGEFQRFQMFYGPSED 120
Query: 121 VPVLANQFSAFVSRPNGNKFSTVLCSARPQKSKDGKNDGIEAWDWNLSGEKSTYHALFPR 180
VPVLANQFSAFVSRPNGN+FSTVLCSARPQKSKDGKNDGIEAWDWNLSGEKSTYHALFPR
Sbjct: 121 VPVLANQFSAFVSRPNGNRFSTVLCSARPQKSKDGKNDGIEAWDWNLSGEKSTYHALFPR 180
Query: 181 AWTIYDGEPDPDLKIVCRQISPIIPHNYKESSFPVSVFTFNLSNKGPTSAEVTLLFTWAN 240
AWTIYDGEPDPDLKIVCRQISPIIPHNYKESSFPVSVFTFNLSNKGPTSAEVTLLFTWAN
Sbjct: 181 AWTIYDGEPDPDLKIVCRQISPIIPHNYKESSFPVSVFTFNLSNKGPTSAEVTLLFTWAN 240
Query: 241 SVGGNSGFTGHHFNSKIG--------AEDGAHGVLLHHKTANGRPTVTYAITAEATDDVH 300
SVGGNSGFTGHHFNSKIG AEDGAHGVLLHHKTANGRPTVTYAITAEATDDVH
Sbjct: 241 SVGGNSGFTGHHFNSKIGVKAEFASSAEDGAHGVLLHHKTANGRPTVTYAITAEATDDVH 300
Query: 301 VSVCPRFVISGDSEGISAKDMWQEVKKHGSFDELGSSGPHEGSKPGSSIGAAIAAKVSIP 360
VSVCPRFVISGDSEGISAKDMWQEVK HGSFD+ G+SGPHE SKPGSSIGAAIAAKVSIP
Sbjct: 301 VSVCPRFVISGDSEGISAKDMWQEVKNHGSFDKFGNSGPHEESKPGSSIGAAIAAKVSIP 360
Query: 361 SSSTRTVTFSLAWDCPEVKFDGKTYHRRYTNFYGTLGEAAKTIARDAILEHGKWEGQIEA 420
SSST TVTFSLAWDCPEVKFDGKTYHRRYTNFYGTLGEAAKTI RDAILEHGKWEGQIEA
Sbjct: 361 SSSTCTVTFSLAWDCPEVKFDGKTYHRRYTNFYGTLGEAAKTIGRDAILEHGKWEGQIEA 420
Query: 421 WQRPIVEDKRLPEWYPVTLFNELYFLNSGGTMWTDGLPPLQNLSTISHQKIFLERTKSEP 480
WQRPIVEDKRLPEWYPVTLFNELYFLNSGGTMWTDGLPPLQNLSTISHQKIFL+RTKSEP
Sbjct: 421 WQRPIVEDKRLPEWYPVTLFNELYFLNSGGTMWTDGLPPLQNLSTISHQKIFLQRTKSEP 480
Query: 481 NGAPNGDHRLDVAVEILERMSQIFDQAHGGAGPSNAALGTRLLPTLQENVGQLLLVEGSE 540
NGAPNGDHRLDVAVEILERMSQIFDQAHGGAGPSNAALGTRLLPTLQENVGQLLLVEGSE
Sbjct: 481 NGAPNGDHRLDVAVEILERMSQIFDQAHGGAGPSNAALGTRLLPTLQENVGQLLLVEGSE 540
Query: 541 YLMWNTFDVHFYSSFALIMLFPKLELGIQRDFAAAVLMHDPRKAKIMSDGKYYPRKVIGA 600
YLMWNTFDVHFYSSFALIMLFPKLELGIQRDFAAAVLMHDPRKAKIMSDGKYYPRKVIGA
Sbjct: 541 YLMWNTFDVHFYSSFALIMLFPKLELGIQRDFAAAVLMHDPRKAKIMSDGKYYPRKVIGA 600
Query: 601 VPHDIGFNDPWFEVNAYNLLNVTRWKDLGSKFVLQVYRDVVATGDKNFAKSVWPSVYVAL 660
VPHDIGFNDPWFEVNAYNLLNVTRWKDLGSKFVLQVYRDVVATGDKNFAKSVWPSVYVAL
Sbjct: 601 VPHDIGFNDPWFEVNAYNLLNVTRWKDLGSKFVLQVYRDVVATGDKNFAKSVWPSVYVAL 660
Query: 661 AFMDQFDKDKDGMIENEGFPDQTYDVWTVKGVSAYCGGLWVAALQAASALAYEVDDEAAA 720
AFMDQFDKDKDGMIENEGFPDQTYDVWTVKGVSAYCGGLWVAALQAASALAYEVDDEAAA
Sbjct: 661 AFMDQFDKDKDGMIENEGFPDQTYDVWTVKGVSAYCGGLWVAALQAASALAYEVDDEAAA 720
Query: 721 HYFWIKYQKARGVYDTLWNGSYFNYDNSGGPWSSSIQADQLAGQWYARACGLCPIVDEEK 780
HYFWIKYQKARGVYDTLWNGSYFNYDNS GPWSSSIQADQLAGQWYARACGLCPIVDEEK
Sbjct: 721 HYFWIKYQKARGVYDTLWNGSYFNYDNSSGPWSSSIQADQLAGQWYARACGLCPIVDEEK 780
Query: 781 IRSTLEKIFNFNVMKVKGGTRGAVNGMFPDGRVDRSILQPKEIWAGVTYSVAASMIQEGM 840
IRSTLEKIFNFNVMKVKGGTRGAVNGMFPDGRVDRSILQPKEIWAGVTYSVAASMIQEGM
Sbjct: 781 IRSTLEKIFNFNVMKVKGGTRGAVNGMFPDGRVDRSILQPKEIWAGVTYSVAASMIQEGM 840
Query: 841 VETGFQTAMGIHQAAWGQDGLGYSFQTPEAWDIDDKYRSLGYMRPLAIWAMQWAISKPTL 900
VETGFQTAMGIHQA WGQDGLGYSFQTPEAWDIDDKYRSLGYMRPLAIWAMQWAISKPTL
Sbjct: 841 VETGFQTAMGIHQAVWGQDGLGYSFQTPEAWDIDDKYRSLGYMRPLAIWAMQWAISKPTL 900
Query: 901 LKKHKVPRKELSESEESCLFSTQHAAFLKVASLLKLPSEEAKPRSVVEVAYDFICKRSA 952
LKKHKVPRKELSESEESCLF+TQHAAFLKVASLLKLPSEEAKPRSVVEVAYDFICKRSA
Sbjct: 901 LKKHKVPRKELSESEESCLFATQHAAFLKVASLLKLPSEEAKPRSVVEVAYDFICKRSA 959
BLAST of CmoCh01G009010 vs. NCBI nr
Match:
XP_022948947.1 (non-lysosomal glucosylceramidase-like isoform X3 [Cucurbita moschata])
HSP 1 Score: 1976.1 bits (5118), Expect = 0.0e+00
Identity = 951/951 (100.00%), Postives = 951/951 (100.00%), Query Frame = 0
Query: 1 MENGCKEDADNGASSTMVVDPAKLPSLTWQRKLDFTGKSPESFSFTLNDKWHLGPLGYRL 60
MENGCKEDADNGASSTMVVDPAKLPSLTWQRKLDFTGKSPESFSFTLNDKWHLGPLGYRL
Sbjct: 1 MENGCKEDADNGASSTMVVDPAKLPSLTWQRKLDFTGKSPESFSFTLNDKWHLGPLGYRL 60
Query: 61 WRHGKEEAAKGRIPLYDFFASPPITCYHGVSLGGIGAGSIGRSYRGEFQRFQMFYGPSED 120
WRHGKEEAAKGRIPLYDFFASPPITCYHGVSLGGIGAGSIGRSYRGEFQRFQMFYGPSED
Sbjct: 61 WRHGKEEAAKGRIPLYDFFASPPITCYHGVSLGGIGAGSIGRSYRGEFQRFQMFYGPSED 120
Query: 121 VPVLANQFSAFVSRPNGNKFSTVLCSARPQKSKDGKNDGIEAWDWNLSGEKSTYHALFPR 180
VPVLANQFSAFVSRPNGNKFSTVLCSARPQKSKDGKNDGIEAWDWNLSGEKSTYHALFPR
Sbjct: 121 VPVLANQFSAFVSRPNGNKFSTVLCSARPQKSKDGKNDGIEAWDWNLSGEKSTYHALFPR 180
Query: 181 AWTIYDGEPDPDLKIVCRQISPIIPHNYKESSFPVSVFTFNLSNKGPTSAEVTLLFTWAN 240
AWTIYDGEPDPDLKIVCRQISPIIPHNYKESSFPVSVFTFNLSNKGPTSAEVTLLFTWAN
Sbjct: 181 AWTIYDGEPDPDLKIVCRQISPIIPHNYKESSFPVSVFTFNLSNKGPTSAEVTLLFTWAN 240
Query: 241 SVGGNSGFTGHHFNSKIGAEDGAHGVLLHHKTANGRPTVTYAITAEATDDVHVSVCPRFV 300
SVGGNSGFTGHHFNSKIGAEDGAHGVLLHHKTANGRPTVTYAITAEATDDVHVSVCPRFV
Sbjct: 241 SVGGNSGFTGHHFNSKIGAEDGAHGVLLHHKTANGRPTVTYAITAEATDDVHVSVCPRFV 300
Query: 301 ISGDSEGISAKDMWQEVKKHGSFDELGSSGPHEGSKPGSSIGAAIAAKVSIPSSSTRTVT 360
ISGDSEGISAKDMWQEVKKHGSFDELGSSGPHEGSKPGSSIGAAIAAKVSIPSSSTRTVT
Sbjct: 301 ISGDSEGISAKDMWQEVKKHGSFDELGSSGPHEGSKPGSSIGAAIAAKVSIPSSSTRTVT 360
Query: 361 FSLAWDCPEVKFDGKTYHRRYTNFYGTLGEAAKTIARDAILEHGKWEGQIEAWQRPIVED 420
FSLAWDCPEVKFDGKTYHRRYTNFYGTLGEAAKTIARDAILEHGKWEGQIEAWQRPIVED
Sbjct: 361 FSLAWDCPEVKFDGKTYHRRYTNFYGTLGEAAKTIARDAILEHGKWEGQIEAWQRPIVED 420
Query: 421 KRLPEWYPVTLFNELYFLNSGGTMWTDGLPPLQNLSTISHQKIFLERTKSEPNGAPNGDH 480
KRLPEWYPVTLFNELYFLNSGGTMWTDGLPPLQNLSTISHQKIFLERTKSEPNGAPNGDH
Sbjct: 421 KRLPEWYPVTLFNELYFLNSGGTMWTDGLPPLQNLSTISHQKIFLERTKSEPNGAPNGDH 480
Query: 481 RLDVAVEILERMSQIFDQAHGGAGPSNAALGTRLLPTLQENVGQLLLVEGSEYLMWNTFD 540
RLDVAVEILERMSQIFDQAHGGAGPSNAALGTRLLPTLQENVGQLLLVEGSEYLMWNTFD
Sbjct: 481 RLDVAVEILERMSQIFDQAHGGAGPSNAALGTRLLPTLQENVGQLLLVEGSEYLMWNTFD 540
Query: 541 VHFYSSFALIMLFPKLELGIQRDFAAAVLMHDPRKAKIMSDGKYYPRKVIGAVPHDIGFN 600
VHFYSSFALIMLFPKLELGIQRDFAAAVLMHDPRKAKIMSDGKYYPRKVIGAVPHDIGFN
Sbjct: 541 VHFYSSFALIMLFPKLELGIQRDFAAAVLMHDPRKAKIMSDGKYYPRKVIGAVPHDIGFN 600
Query: 601 DPWFEVNAYNLLNVTRWKDLGSKFVLQVYRDVVATGDKNFAKSVWPSVYVALAFMDQFDK 660
DPWFEVNAYNLLNVTRWKDLGSKFVLQVYRDVVATGDKNFAKSVWPSVYVALAFMDQFDK
Sbjct: 601 DPWFEVNAYNLLNVTRWKDLGSKFVLQVYRDVVATGDKNFAKSVWPSVYVALAFMDQFDK 660
Query: 661 DKDGMIENEGFPDQTYDVWTVKGVSAYCGGLWVAALQAASALAYEVDDEAAAHYFWIKYQ 720
DKDGMIENEGFPDQTYDVWTVKGVSAYCGGLWVAALQAASALAYEVDDEAAAHYFWIKYQ
Sbjct: 661 DKDGMIENEGFPDQTYDVWTVKGVSAYCGGLWVAALQAASALAYEVDDEAAAHYFWIKYQ 720
Query: 721 KARGVYDTLWNGSYFNYDNSGGPWSSSIQADQLAGQWYARACGLCPIVDEEKIRSTLEKI 780
KARGVYDTLWNGSYFNYDNSGGPWSSSIQADQLAGQWYARACGLCPIVDEEKIRSTLEKI
Sbjct: 721 KARGVYDTLWNGSYFNYDNSGGPWSSSIQADQLAGQWYARACGLCPIVDEEKIRSTLEKI 780
Query: 781 FNFNVMKVKGGTRGAVNGMFPDGRVDRSILQPKEIWAGVTYSVAASMIQEGMVETGFQTA 840
FNFNVMKVKGGTRGAVNGMFPDGRVDRSILQPKEIWAGVTYSVAASMIQEGMVETGFQTA
Sbjct: 781 FNFNVMKVKGGTRGAVNGMFPDGRVDRSILQPKEIWAGVTYSVAASMIQEGMVETGFQTA 840
Query: 841 MGIHQAAWGQDGLGYSFQTPEAWDIDDKYRSLGYMRPLAIWAMQWAISKPTLLKKHKVPR 900
MGIHQAAWGQDGLGYSFQTPEAWDIDDKYRSLGYMRPLAIWAMQWAISKPTLLKKHKVPR
Sbjct: 841 MGIHQAAWGQDGLGYSFQTPEAWDIDDKYRSLGYMRPLAIWAMQWAISKPTLLKKHKVPR 900
Query: 901 KELSESEESCLFSTQHAAFLKVASLLKLPSEEAKPRSVVEVAYDFICKRSA 952
KELSESEESCLFSTQHAAFLKVASLLKLPSEEAKPRSVVEVAYDFICKRSA
Sbjct: 901 KELSESEESCLFSTQHAAFLKVASLLKLPSEEAKPRSVVEVAYDFICKRSA 951
BLAST of CmoCh01G009010 vs. NCBI nr
Match:
KAG6607519.1 (Non-lysosomal glucosylceramidase, partial [Cucurbita argyrosperma subsp. sororia])
HSP 1 Score: 1971.8 bits (5107), Expect = 0.0e+00
Identity = 949/951 (99.79%), Postives = 950/951 (99.89%), Query Frame = 0
Query: 1 MENGCKEDADNGASSTMVVDPAKLPSLTWQRKLDFTGKSPESFSFTLNDKWHLGPLGYRL 60
MENGCKEDADNGASSTMVVDPAKLPSLTWQRKLDFTGKSPESFSFTLNDKWHLGPLGYRL
Sbjct: 1 MENGCKEDADNGASSTMVVDPAKLPSLTWQRKLDFTGKSPESFSFTLNDKWHLGPLGYRL 60
Query: 61 WRHGKEEAAKGRIPLYDFFASPPITCYHGVSLGGIGAGSIGRSYRGEFQRFQMFYGPSED 120
WRHGKEEAAKGRIPLYDFFASPPITCYHGVSLGGIGAGSIGRSYRGEFQRFQMFYGPSED
Sbjct: 61 WRHGKEEAAKGRIPLYDFFASPPITCYHGVSLGGIGAGSIGRSYRGEFQRFQMFYGPSED 120
Query: 121 VPVLANQFSAFVSRPNGNKFSTVLCSARPQKSKDGKNDGIEAWDWNLSGEKSTYHALFPR 180
VPVLANQFSAFVSRPNGNKFSTVLCSARPQKSKDGKNDGIEAWDWNLSGEKSTYHALFPR
Sbjct: 121 VPVLANQFSAFVSRPNGNKFSTVLCSARPQKSKDGKNDGIEAWDWNLSGEKSTYHALFPR 180
Query: 181 AWTIYDGEPDPDLKIVCRQISPIIPHNYKESSFPVSVFTFNLSNKGPTSAEVTLLFTWAN 240
AWTIYDGEPDPDLKIVCRQISPIIPHNYKESSFPVSVFTFNLSNKGPTSAEVTLLFTWAN
Sbjct: 181 AWTIYDGEPDPDLKIVCRQISPIIPHNYKESSFPVSVFTFNLSNKGPTSAEVTLLFTWAN 240
Query: 241 SVGGNSGFTGHHFNSKIGAEDGAHGVLLHHKTANGRPTVTYAITAEATDDVHVSVCPRFV 300
SVGGNSGFTGHHFNSKIGAEDGAHGVLLHHKTANGRPTVTYAITAEATDDV+VSVCPRFV
Sbjct: 241 SVGGNSGFTGHHFNSKIGAEDGAHGVLLHHKTANGRPTVTYAITAEATDDVYVSVCPRFV 300
Query: 301 ISGDSEGISAKDMWQEVKKHGSFDELGSSGPHEGSKPGSSIGAAIAAKVSIPSSSTRTVT 360
ISGDSEGISAKDMWQEVK HGSFDELGSSGPHEGSKPGSSIGAAIAAKVSIPSSSTRTVT
Sbjct: 301 ISGDSEGISAKDMWQEVKNHGSFDELGSSGPHEGSKPGSSIGAAIAAKVSIPSSSTRTVT 360
Query: 361 FSLAWDCPEVKFDGKTYHRRYTNFYGTLGEAAKTIARDAILEHGKWEGQIEAWQRPIVED 420
FSLAWDCPEVKFDGKTYHRRYTNFYGTLGEAAKTIARDAILEHGKWEGQIEAWQRPIVED
Sbjct: 361 FSLAWDCPEVKFDGKTYHRRYTNFYGTLGEAAKTIARDAILEHGKWEGQIEAWQRPIVED 420
Query: 421 KRLPEWYPVTLFNELYFLNSGGTMWTDGLPPLQNLSTISHQKIFLERTKSEPNGAPNGDH 480
KRLPEWYPVTLFNELYFLNSGGTMWTDGLPPLQNLSTISHQKIFLERTKSEPNGAPNGDH
Sbjct: 421 KRLPEWYPVTLFNELYFLNSGGTMWTDGLPPLQNLSTISHQKIFLERTKSEPNGAPNGDH 480
Query: 481 RLDVAVEILERMSQIFDQAHGGAGPSNAALGTRLLPTLQENVGQLLLVEGSEYLMWNTFD 540
RLDVAVEILERMSQIFDQAHGGAGPSNAALGTRLLPTLQENVGQLLLVEGSEYLMWNTFD
Sbjct: 481 RLDVAVEILERMSQIFDQAHGGAGPSNAALGTRLLPTLQENVGQLLLVEGSEYLMWNTFD 540
Query: 541 VHFYSSFALIMLFPKLELGIQRDFAAAVLMHDPRKAKIMSDGKYYPRKVIGAVPHDIGFN 600
VHFYSSFALIMLFPKLELGIQRDFAAAVLMHDPRKAKIMSDGKYYPRKVIGAVPHDIGFN
Sbjct: 541 VHFYSSFALIMLFPKLELGIQRDFAAAVLMHDPRKAKIMSDGKYYPRKVIGAVPHDIGFN 600
Query: 601 DPWFEVNAYNLLNVTRWKDLGSKFVLQVYRDVVATGDKNFAKSVWPSVYVALAFMDQFDK 660
DPWFEVNAYNLLNVTRWKDLGSKFVLQVYRDVVATGDKNFAKSVWPSVYVALAFMDQFDK
Sbjct: 601 DPWFEVNAYNLLNVTRWKDLGSKFVLQVYRDVVATGDKNFAKSVWPSVYVALAFMDQFDK 660
Query: 661 DKDGMIENEGFPDQTYDVWTVKGVSAYCGGLWVAALQAASALAYEVDDEAAAHYFWIKYQ 720
DKDGMIENEGFPDQTYDVWTVKGVSAYCGGLWVAALQAASALAYEVDDEAAAHYFWIKYQ
Sbjct: 661 DKDGMIENEGFPDQTYDVWTVKGVSAYCGGLWVAALQAASALAYEVDDEAAAHYFWIKYQ 720
Query: 721 KARGVYDTLWNGSYFNYDNSGGPWSSSIQADQLAGQWYARACGLCPIVDEEKIRSTLEKI 780
KARGVYDTLWNGSYFNYDNSGGPWSSSIQADQLAGQWYARACGLCPIVDEEKIRSTLEKI
Sbjct: 721 KARGVYDTLWNGSYFNYDNSGGPWSSSIQADQLAGQWYARACGLCPIVDEEKIRSTLEKI 780
Query: 781 FNFNVMKVKGGTRGAVNGMFPDGRVDRSILQPKEIWAGVTYSVAASMIQEGMVETGFQTA 840
FNFNVMKVKGGTRGAVNGMFPDGRVDRSILQPKEIWAGVTYSVAASMIQEGMVETGFQTA
Sbjct: 781 FNFNVMKVKGGTRGAVNGMFPDGRVDRSILQPKEIWAGVTYSVAASMIQEGMVETGFQTA 840
Query: 841 MGIHQAAWGQDGLGYSFQTPEAWDIDDKYRSLGYMRPLAIWAMQWAISKPTLLKKHKVPR 900
MGIHQAAWGQDGLGYSFQTPEAWDIDDKYRSLGYMRPLAIWAMQWAISKPTLLKKHKVPR
Sbjct: 841 MGIHQAAWGQDGLGYSFQTPEAWDIDDKYRSLGYMRPLAIWAMQWAISKPTLLKKHKVPR 900
Query: 901 KELSESEESCLFSTQHAAFLKVASLLKLPSEEAKPRSVVEVAYDFICKRSA 952
KELSESEESCLFSTQHAAFLKVASLLKLPSEEAKPRSVVEVAYDFICKRSA
Sbjct: 901 KELSESEESCLFSTQHAAFLKVASLLKLPSEEAKPRSVVEVAYDFICKRSA 951
BLAST of CmoCh01G009010 vs. NCBI nr
Match:
XP_022948945.1 (non-lysosomal glucosylceramidase-like isoform X1 [Cucurbita moschata])
HSP 1 Score: 1968.7 bits (5099), Expect = 0.0e+00
Identity = 951/959 (99.17%), Postives = 951/959 (99.17%), Query Frame = 0
Query: 1 MENGCKEDADNGASSTMVVDPAKLPSLTWQRKLDFTGKSPESFSFTLNDKWHLGPLGYRL 60
MENGCKEDADNGASSTMVVDPAKLPSLTWQRKLDFTGKSPESFSFTLNDKWHLGPLGYRL
Sbjct: 1 MENGCKEDADNGASSTMVVDPAKLPSLTWQRKLDFTGKSPESFSFTLNDKWHLGPLGYRL 60
Query: 61 WRHGKEEAAKGRIPLYDFFASPPITCYHGVSLGGIGAGSIGRSYRGEFQRFQMFYGPSED 120
WRHGKEEAAKGRIPLYDFFASPPITCYHGVSLGGIGAGSIGRSYRGEFQRFQMFYGPSED
Sbjct: 61 WRHGKEEAAKGRIPLYDFFASPPITCYHGVSLGGIGAGSIGRSYRGEFQRFQMFYGPSED 120
Query: 121 VPVLANQFSAFVSRPNGNKFSTVLCSARPQKSKDGKNDGIEAWDWNLSGEKSTYHALFPR 180
VPVLANQFSAFVSRPNGNKFSTVLCSARPQKSKDGKNDGIEAWDWNLSGEKSTYHALFPR
Sbjct: 121 VPVLANQFSAFVSRPNGNKFSTVLCSARPQKSKDGKNDGIEAWDWNLSGEKSTYHALFPR 180
Query: 181 AWTIYDGEPDPDLKIVCRQISPIIPHNYKESSFPVSVFTFNLSNKGPTSAEVTLLFTWAN 240
AWTIYDGEPDPDLKIVCRQISPIIPHNYKESSFPVSVFTFNLSNKGPTSAEVTLLFTWAN
Sbjct: 181 AWTIYDGEPDPDLKIVCRQISPIIPHNYKESSFPVSVFTFNLSNKGPTSAEVTLLFTWAN 240
Query: 241 SVGGNSGFTGHHFNSKIG--------AEDGAHGVLLHHKTANGRPTVTYAITAEATDDVH 300
SVGGNSGFTGHHFNSKIG AEDGAHGVLLHHKTANGRPTVTYAITAEATDDVH
Sbjct: 241 SVGGNSGFTGHHFNSKIGVKAEFVSSAEDGAHGVLLHHKTANGRPTVTYAITAEATDDVH 300
Query: 301 VSVCPRFVISGDSEGISAKDMWQEVKKHGSFDELGSSGPHEGSKPGSSIGAAIAAKVSIP 360
VSVCPRFVISGDSEGISAKDMWQEVKKHGSFDELGSSGPHEGSKPGSSIGAAIAAKVSIP
Sbjct: 301 VSVCPRFVISGDSEGISAKDMWQEVKKHGSFDELGSSGPHEGSKPGSSIGAAIAAKVSIP 360
Query: 361 SSSTRTVTFSLAWDCPEVKFDGKTYHRRYTNFYGTLGEAAKTIARDAILEHGKWEGQIEA 420
SSSTRTVTFSLAWDCPEVKFDGKTYHRRYTNFYGTLGEAAKTIARDAILEHGKWEGQIEA
Sbjct: 361 SSSTRTVTFSLAWDCPEVKFDGKTYHRRYTNFYGTLGEAAKTIARDAILEHGKWEGQIEA 420
Query: 421 WQRPIVEDKRLPEWYPVTLFNELYFLNSGGTMWTDGLPPLQNLSTISHQKIFLERTKSEP 480
WQRPIVEDKRLPEWYPVTLFNELYFLNSGGTMWTDGLPPLQNLSTISHQKIFLERTKSEP
Sbjct: 421 WQRPIVEDKRLPEWYPVTLFNELYFLNSGGTMWTDGLPPLQNLSTISHQKIFLERTKSEP 480
Query: 481 NGAPNGDHRLDVAVEILERMSQIFDQAHGGAGPSNAALGTRLLPTLQENVGQLLLVEGSE 540
NGAPNGDHRLDVAVEILERMSQIFDQAHGGAGPSNAALGTRLLPTLQENVGQLLLVEGSE
Sbjct: 481 NGAPNGDHRLDVAVEILERMSQIFDQAHGGAGPSNAALGTRLLPTLQENVGQLLLVEGSE 540
Query: 541 YLMWNTFDVHFYSSFALIMLFPKLELGIQRDFAAAVLMHDPRKAKIMSDGKYYPRKVIGA 600
YLMWNTFDVHFYSSFALIMLFPKLELGIQRDFAAAVLMHDPRKAKIMSDGKYYPRKVIGA
Sbjct: 541 YLMWNTFDVHFYSSFALIMLFPKLELGIQRDFAAAVLMHDPRKAKIMSDGKYYPRKVIGA 600
Query: 601 VPHDIGFNDPWFEVNAYNLLNVTRWKDLGSKFVLQVYRDVVATGDKNFAKSVWPSVYVAL 660
VPHDIGFNDPWFEVNAYNLLNVTRWKDLGSKFVLQVYRDVVATGDKNFAKSVWPSVYVAL
Sbjct: 601 VPHDIGFNDPWFEVNAYNLLNVTRWKDLGSKFVLQVYRDVVATGDKNFAKSVWPSVYVAL 660
Query: 661 AFMDQFDKDKDGMIENEGFPDQTYDVWTVKGVSAYCGGLWVAALQAASALAYEVDDEAAA 720
AFMDQFDKDKDGMIENEGFPDQTYDVWTVKGVSAYCGGLWVAALQAASALAYEVDDEAAA
Sbjct: 661 AFMDQFDKDKDGMIENEGFPDQTYDVWTVKGVSAYCGGLWVAALQAASALAYEVDDEAAA 720
Query: 721 HYFWIKYQKARGVYDTLWNGSYFNYDNSGGPWSSSIQADQLAGQWYARACGLCPIVDEEK 780
HYFWIKYQKARGVYDTLWNGSYFNYDNSGGPWSSSIQADQLAGQWYARACGLCPIVDEEK
Sbjct: 721 HYFWIKYQKARGVYDTLWNGSYFNYDNSGGPWSSSIQADQLAGQWYARACGLCPIVDEEK 780
Query: 781 IRSTLEKIFNFNVMKVKGGTRGAVNGMFPDGRVDRSILQPKEIWAGVTYSVAASMIQEGM 840
IRSTLEKIFNFNVMKVKGGTRGAVNGMFPDGRVDRSILQPKEIWAGVTYSVAASMIQEGM
Sbjct: 781 IRSTLEKIFNFNVMKVKGGTRGAVNGMFPDGRVDRSILQPKEIWAGVTYSVAASMIQEGM 840
Query: 841 VETGFQTAMGIHQAAWGQDGLGYSFQTPEAWDIDDKYRSLGYMRPLAIWAMQWAISKPTL 900
VETGFQTAMGIHQAAWGQDGLGYSFQTPEAWDIDDKYRSLGYMRPLAIWAMQWAISKPTL
Sbjct: 841 VETGFQTAMGIHQAAWGQDGLGYSFQTPEAWDIDDKYRSLGYMRPLAIWAMQWAISKPTL 900
Query: 901 LKKHKVPRKELSESEESCLFSTQHAAFLKVASLLKLPSEEAKPRSVVEVAYDFICKRSA 952
LKKHKVPRKELSESEESCLFSTQHAAFLKVASLLKLPSEEAKPRSVVEVAYDFICKRSA
Sbjct: 901 LKKHKVPRKELSESEESCLFSTQHAAFLKVASLLKLPSEEAKPRSVVEVAYDFICKRSA 959
BLAST of CmoCh01G009010 vs. NCBI nr
Match:
XP_023524726.1 (non-lysosomal glucosylceramidase-like isoform X4 [Cucurbita pepo subsp. pepo])
HSP 1 Score: 1965.7 bits (5091), Expect = 0.0e+00
Identity = 945/951 (99.37%), Postives = 949/951 (99.79%), Query Frame = 0
Query: 1 MENGCKEDADNGASSTMVVDPAKLPSLTWQRKLDFTGKSPESFSFTLNDKWHLGPLGYRL 60
MENGCKE+ADNGASSTMVVDPAKLPSLTWQRKLDFTGKSPESFSFTLNDKWHLGPLGYRL
Sbjct: 1 MENGCKEEADNGASSTMVVDPAKLPSLTWQRKLDFTGKSPESFSFTLNDKWHLGPLGYRL 60
Query: 61 WRHGKEEAAKGRIPLYDFFASPPITCYHGVSLGGIGAGSIGRSYRGEFQRFQMFYGPSED 120
WRHGKEEAAKGRIPLYDFFASPPITCYHGVSLGGIGAGSIGRSYRGEFQRFQMFYGPSED
Sbjct: 61 WRHGKEEAAKGRIPLYDFFASPPITCYHGVSLGGIGAGSIGRSYRGEFQRFQMFYGPSED 120
Query: 121 VPVLANQFSAFVSRPNGNKFSTVLCSARPQKSKDGKNDGIEAWDWNLSGEKSTYHALFPR 180
VPVLANQFSAFVSRPNGNKFSTVLCSARPQKSKDGKNDGIEAWDWNLSGEKSTYHALFPR
Sbjct: 121 VPVLANQFSAFVSRPNGNKFSTVLCSARPQKSKDGKNDGIEAWDWNLSGEKSTYHALFPR 180
Query: 181 AWTIYDGEPDPDLKIVCRQISPIIPHNYKESSFPVSVFTFNLSNKGPTSAEVTLLFTWAN 240
AWTIYDGEPDPDLKIVCRQISPIIPHNYKESSFPVSVFTFNLSNKGPTSAEVTLLFTWAN
Sbjct: 181 AWTIYDGEPDPDLKIVCRQISPIIPHNYKESSFPVSVFTFNLSNKGPTSAEVTLLFTWAN 240
Query: 241 SVGGNSGFTGHHFNSKIGAEDGAHGVLLHHKTANGRPTVTYAITAEATDDVHVSVCPRFV 300
SVGGNSGFTGHHFNSKIGAEDGAHGVLLHHKTANGRPTVTYAITAEATDDVHVSVCPRFV
Sbjct: 241 SVGGNSGFTGHHFNSKIGAEDGAHGVLLHHKTANGRPTVTYAITAEATDDVHVSVCPRFV 300
Query: 301 ISGDSEGISAKDMWQEVKKHGSFDELGSSGPHEGSKPGSSIGAAIAAKVSIPSSSTRTVT 360
ISGDSEGISAKDMWQEVK HGSFDELGSSGPHEGSKPGSSIGAAIAAKV+IPSSSTRTVT
Sbjct: 301 ISGDSEGISAKDMWQEVKNHGSFDELGSSGPHEGSKPGSSIGAAIAAKVTIPSSSTRTVT 360
Query: 361 FSLAWDCPEVKFDGKTYHRRYTNFYGTLGEAAKTIARDAILEHGKWEGQIEAWQRPIVED 420
FSLAWDCPEVKFDGKTYHRRYTNFYGTLGEAAKTIARDAILEHGKWEGQIEAWQRPIVED
Sbjct: 361 FSLAWDCPEVKFDGKTYHRRYTNFYGTLGEAAKTIARDAILEHGKWEGQIEAWQRPIVED 420
Query: 421 KRLPEWYPVTLFNELYFLNSGGTMWTDGLPPLQNLSTISHQKIFLERTKSEPNGAPNGDH 480
KRLPEWYPVTLFNELYFLNSGGTMWTDGLPPLQNLSTISHQKIFLERTKSE NGAPNGDH
Sbjct: 421 KRLPEWYPVTLFNELYFLNSGGTMWTDGLPPLQNLSTISHQKIFLERTKSESNGAPNGDH 480
Query: 481 RLDVAVEILERMSQIFDQAHGGAGPSNAALGTRLLPTLQENVGQLLLVEGSEYLMWNTFD 540
RLDVAVEILERMSQIFDQAHGGAGPSNAALGTRLLPTLQENVGQLLLVEGSEYLMWNTFD
Sbjct: 481 RLDVAVEILERMSQIFDQAHGGAGPSNAALGTRLLPTLQENVGQLLLVEGSEYLMWNTFD 540
Query: 541 VHFYSSFALIMLFPKLELGIQRDFAAAVLMHDPRKAKIMSDGKYYPRKVIGAVPHDIGFN 600
VHFYSSFALIMLFPKLELGIQRDFAAAVLMHDPRKAKIMSDGKYYPRKVIGAVPHDIGFN
Sbjct: 541 VHFYSSFALIMLFPKLELGIQRDFAAAVLMHDPRKAKIMSDGKYYPRKVIGAVPHDIGFN 600
Query: 601 DPWFEVNAYNLLNVTRWKDLGSKFVLQVYRDVVATGDKNFAKSVWPSVYVALAFMDQFDK 660
DPWFEVNAYNLLNVTRWKDLGSKFVLQVYRDVVATGDKNFAKSVWPSVYVALAFMDQFDK
Sbjct: 601 DPWFEVNAYNLLNVTRWKDLGSKFVLQVYRDVVATGDKNFAKSVWPSVYVALAFMDQFDK 660
Query: 661 DKDGMIENEGFPDQTYDVWTVKGVSAYCGGLWVAALQAASALAYEVDDEAAAHYFWIKYQ 720
DKDGMIENEGFPDQTYDVWTVKGVSAYCGGLWVAALQAASALAYEVDDEAAAHYFWIKYQ
Sbjct: 661 DKDGMIENEGFPDQTYDVWTVKGVSAYCGGLWVAALQAASALAYEVDDEAAAHYFWIKYQ 720
Query: 721 KARGVYDTLWNGSYFNYDNSGGPWSSSIQADQLAGQWYARACGLCPIVDEEKIRSTLEKI 780
KARGVYDTLWNGSYFNYDNSGGPWSSSIQADQLAG+WYARACGLCPIVDEEKIRSTLEKI
Sbjct: 721 KARGVYDTLWNGSYFNYDNSGGPWSSSIQADQLAGKWYARACGLCPIVDEEKIRSTLEKI 780
Query: 781 FNFNVMKVKGGTRGAVNGMFPDGRVDRSILQPKEIWAGVTYSVAASMIQEGMVETGFQTA 840
FNFNVMKVKGGTRGAVNGMFPDGRVDRSILQPKEIWAGVTYSVAASMIQEGMVETGFQTA
Sbjct: 781 FNFNVMKVKGGTRGAVNGMFPDGRVDRSILQPKEIWAGVTYSVAASMIQEGMVETGFQTA 840
Query: 841 MGIHQAAWGQDGLGYSFQTPEAWDIDDKYRSLGYMRPLAIWAMQWAISKPTLLKKHKVPR 900
MGIHQAAWGQDGLGYSFQTPEAWDIDDKYRSLGYMRPLAIWAMQWAISKPTLLKKHKVPR
Sbjct: 841 MGIHQAAWGQDGLGYSFQTPEAWDIDDKYRSLGYMRPLAIWAMQWAISKPTLLKKHKVPR 900
Query: 901 KELSESEESCLFSTQHAAFLKVASLLKLPSEEAKPRSVVEVAYDFICKRSA 952
KELSESEESCLF+TQHAAFLKVASLLKLPSEEAKPRSVVEVAYDFICKRSA
Sbjct: 901 KELSESEESCLFATQHAAFLKVASLLKLPSEEAKPRSVVEVAYDFICKRSA 951
BLAST of CmoCh01G009010 vs. NCBI nr
Match:
XP_022948946.1 (non-lysosomal glucosylceramidase-like isoform X2 [Cucurbita moschata])
HSP 1 Score: 1962.2 bits (5082), Expect = 0.0e+00
Identity = 950/959 (99.06%), Postives = 950/959 (99.06%), Query Frame = 0
Query: 1 MENGCKEDADNGASSTMVVDPAKLPSLTWQRKLDFTGKSPESFSFTLNDKWHLGPLGYRL 60
MENGCKEDADNGASSTM VDPAKLPSLTWQRKLDFTGKSPESFSFTLNDKWHLGPLGYRL
Sbjct: 1 MENGCKEDADNGASSTM-VDPAKLPSLTWQRKLDFTGKSPESFSFTLNDKWHLGPLGYRL 60
Query: 61 WRHGKEEAAKGRIPLYDFFASPPITCYHGVSLGGIGAGSIGRSYRGEFQRFQMFYGPSED 120
WRHGKEEAAKGRIPLYDFFASPPITCYHGVSLGGIGAGSIGRSYRGEFQRFQMFYGPSED
Sbjct: 61 WRHGKEEAAKGRIPLYDFFASPPITCYHGVSLGGIGAGSIGRSYRGEFQRFQMFYGPSED 120
Query: 121 VPVLANQFSAFVSRPNGNKFSTVLCSARPQKSKDGKNDGIEAWDWNLSGEKSTYHALFPR 180
VPVLANQFSAFVSRPNGNKFSTVLCSARPQKSKDGKNDGIEAWDWNLSGEKSTYHALFPR
Sbjct: 121 VPVLANQFSAFVSRPNGNKFSTVLCSARPQKSKDGKNDGIEAWDWNLSGEKSTYHALFPR 180
Query: 181 AWTIYDGEPDPDLKIVCRQISPIIPHNYKESSFPVSVFTFNLSNKGPTSAEVTLLFTWAN 240
AWTIYDGEPDPDLKIVCRQISPIIPHNYKESSFPVSVFTFNLSNKGPTSAEVTLLFTWAN
Sbjct: 181 AWTIYDGEPDPDLKIVCRQISPIIPHNYKESSFPVSVFTFNLSNKGPTSAEVTLLFTWAN 240
Query: 241 SVGGNSGFTGHHFNSKIG--------AEDGAHGVLLHHKTANGRPTVTYAITAEATDDVH 300
SVGGNSGFTGHHFNSKIG AEDGAHGVLLHHKTANGRPTVTYAITAEATDDVH
Sbjct: 241 SVGGNSGFTGHHFNSKIGVKAEFVSSAEDGAHGVLLHHKTANGRPTVTYAITAEATDDVH 300
Query: 301 VSVCPRFVISGDSEGISAKDMWQEVKKHGSFDELGSSGPHEGSKPGSSIGAAIAAKVSIP 360
VSVCPRFVISGDSEGISAKDMWQEVKKHGSFDELGSSGPHEGSKPGSSIGAAIAAKVSIP
Sbjct: 301 VSVCPRFVISGDSEGISAKDMWQEVKKHGSFDELGSSGPHEGSKPGSSIGAAIAAKVSIP 360
Query: 361 SSSTRTVTFSLAWDCPEVKFDGKTYHRRYTNFYGTLGEAAKTIARDAILEHGKWEGQIEA 420
SSSTRTVTFSLAWDCPEVKFDGKTYHRRYTNFYGTLGEAAKTIARDAILEHGKWEGQIEA
Sbjct: 361 SSSTRTVTFSLAWDCPEVKFDGKTYHRRYTNFYGTLGEAAKTIARDAILEHGKWEGQIEA 420
Query: 421 WQRPIVEDKRLPEWYPVTLFNELYFLNSGGTMWTDGLPPLQNLSTISHQKIFLERTKSEP 480
WQRPIVEDKRLPEWYPVTLFNELYFLNSGGTMWTDGLPPLQNLSTISHQKIFLERTKSEP
Sbjct: 421 WQRPIVEDKRLPEWYPVTLFNELYFLNSGGTMWTDGLPPLQNLSTISHQKIFLERTKSEP 480
Query: 481 NGAPNGDHRLDVAVEILERMSQIFDQAHGGAGPSNAALGTRLLPTLQENVGQLLLVEGSE 540
NGAPNGDHRLDVAVEILERMSQIFDQAHGGAGPSNAALGTRLLPTLQENVGQLLLVEGSE
Sbjct: 481 NGAPNGDHRLDVAVEILERMSQIFDQAHGGAGPSNAALGTRLLPTLQENVGQLLLVEGSE 540
Query: 541 YLMWNTFDVHFYSSFALIMLFPKLELGIQRDFAAAVLMHDPRKAKIMSDGKYYPRKVIGA 600
YLMWNTFDVHFYSSFALIMLFPKLELGIQRDFAAAVLMHDPRKAKIMSDGKYYPRKVIGA
Sbjct: 541 YLMWNTFDVHFYSSFALIMLFPKLELGIQRDFAAAVLMHDPRKAKIMSDGKYYPRKVIGA 600
Query: 601 VPHDIGFNDPWFEVNAYNLLNVTRWKDLGSKFVLQVYRDVVATGDKNFAKSVWPSVYVAL 660
VPHDIGFNDPWFEVNAYNLLNVTRWKDLGSKFVLQVYRDVVATGDKNFAKSVWPSVYVAL
Sbjct: 601 VPHDIGFNDPWFEVNAYNLLNVTRWKDLGSKFVLQVYRDVVATGDKNFAKSVWPSVYVAL 660
Query: 661 AFMDQFDKDKDGMIENEGFPDQTYDVWTVKGVSAYCGGLWVAALQAASALAYEVDDEAAA 720
AFMDQFDKDKDGMIENEGFPDQTYDVWTVKGVSAYCGGLWVAALQAASALAYEVDDEAAA
Sbjct: 661 AFMDQFDKDKDGMIENEGFPDQTYDVWTVKGVSAYCGGLWVAALQAASALAYEVDDEAAA 720
Query: 721 HYFWIKYQKARGVYDTLWNGSYFNYDNSGGPWSSSIQADQLAGQWYARACGLCPIVDEEK 780
HYFWIKYQKARGVYDTLWNGSYFNYDNSGGPWSSSIQADQLAGQWYARACGLCPIVDEEK
Sbjct: 721 HYFWIKYQKARGVYDTLWNGSYFNYDNSGGPWSSSIQADQLAGQWYARACGLCPIVDEEK 780
Query: 781 IRSTLEKIFNFNVMKVKGGTRGAVNGMFPDGRVDRSILQPKEIWAGVTYSVAASMIQEGM 840
IRSTLEKIFNFNVMKVKGGTRGAVNGMFPDGRVDRSILQPKEIWAGVTYSVAASMIQEGM
Sbjct: 781 IRSTLEKIFNFNVMKVKGGTRGAVNGMFPDGRVDRSILQPKEIWAGVTYSVAASMIQEGM 840
Query: 841 VETGFQTAMGIHQAAWGQDGLGYSFQTPEAWDIDDKYRSLGYMRPLAIWAMQWAISKPTL 900
VETGFQTAMGIHQAAWGQDGLGYSFQTPEAWDIDDKYRSLGYMRPLAIWAMQWAISKPTL
Sbjct: 841 VETGFQTAMGIHQAAWGQDGLGYSFQTPEAWDIDDKYRSLGYMRPLAIWAMQWAISKPTL 900
Query: 901 LKKHKVPRKELSESEESCLFSTQHAAFLKVASLLKLPSEEAKPRSVVEVAYDFICKRSA 952
LKKHKVPRKELSESEESCLFSTQHAAFLKVASLLKLPSEEAKPRSVVEVAYDFICKRSA
Sbjct: 901 LKKHKVPRKELSESEESCLFSTQHAAFLKVASLLKLPSEEAKPRSVVEVAYDFICKRSA 958
BLAST of CmoCh01G009010 vs. TAIR 10
Match:
AT5G49900.1 (Beta-glucosidase, GBA2 type family protein )
HSP 1 Score: 1211.8 bits (3134), Expect = 0.0e+00
Identity = 585/946 (61.84%), Postives = 721/946 (76.22%), Query Frame = 0
Query: 7 EDADNGASSTMVVDPAKLPSLTWQRKLDFTGKSPESFSFTLNDKWHLGPLGYRLWRHGKE 66
ED SS VDPA SLTWQRK+D K+P F+ ++ + + L P+G RLW +E
Sbjct: 11 EDVKPLNSSDTKVDPAVPASLTWQRKIDSDVKAPREFNLSVKEIFQLAPVGIRLWFLCRE 70
Query: 67 EAAKGRIPLYDFFASPPITCYHGVSLGGIGAGSIGRSYRGEFQRFQMFYGPSEDVPVLAN 126
EAAKGR+ D F+ +T HGV LGGIGAGSIGRS++GEFQR+Q+F ED PVLAN
Sbjct: 71 EAAKGRLAFIDPFSKHSVTSSHGVPLGGIGAGSIGRSFKGEFQRWQLFPPKCEDEPVLAN 130
Query: 127 QFSAFVSRPNGNKFSTVLCSARPQKSKDGKNDGIEAWDWNLSGEKSTYHALFPRAWTIYD 186
QFSAFVSR NG K+S+VLC P+ K GI +WDWNL G+KSTYHAL+PR+WT+Y+
Sbjct: 131 QFSAFVSRANGKKYSSVLCPRNPKLDKQDSESGIGSWDWNLKGDKSTYHALYPRSWTMYE 190
Query: 187 GEPDPDLKIVCRQISPIIPHNYKESSFPVSVFTFNLSNKGPTSAEVTLLFTWANSVGGNS 246
GEPDP+L+IVCRQ+SP IPHNYKESSFPVSVFTF L N G T+A+VTLLFTWANSVGG+S
Sbjct: 191 GEPDPELRIVCRQVSPFIPHNYKESSFPVSVFTFTLHNLGNTTADVTLLFTWANSVGGDS 250
Query: 247 GFTGHHFNSKIGAEDGAHGVLLHHKTANGRPTVTYAITAEATDDVHVSVCPRFVISGDSE 306
F+G H+NSKI DG GVLLHHKTANG P+++YAI+A+ATD V VS CP F++SG +
Sbjct: 251 EFSGGHYNSKITMNDGVQGVLLHHKTANGLPSLSYAISAQATDGVSVSACPFFIVSGKQD 310
Query: 307 GISAKDMWQEVKKHGSFDELGSSGPHEGSKPGSSIGAAIAAKVSIPSSSTRTVTFSLAWD 366
GI+AKDMWQ VK++GSFD L +S S GSSIGAA+AA V++ +R VTFSLAWD
Sbjct: 311 GITAKDMWQAVKENGSFDHLKASEASMQSDHGSSIGAAVAASVTVLPGESRIVTFSLAWD 370
Query: 367 CPEVKF-DGKTYHRRYTNFYGTLGEAAKTIARDAILEHGKWEGQIEAWQRPIVEDKRLPE 426
CPEV+F GK Y RRYT FYG G+AA IA DAIL H +WE IE WQRPI+EDKRLP
Sbjct: 371 CPEVQFPSGKIYSRRYTKFYGNNGDAAAQIAHDAILGHSQWESWIEDWQRPILEDKRLPA 430
Query: 427 WYPVTLFNELYFLNSGGTMWTDGLPPLQNLSTISHQKIFLERTKSEPNGAPNGDHRLDVA 486
WYPVTLFNELY+LNSGGT+WTDG P+ +L+ + +K L++++ + H+ D A
Sbjct: 431 WYPVTLFNELYYLNSGGTLWTDGSSPVHSLAGVREKKFSLDKSQLGLKNDIDVPHQNDTA 490
Query: 487 VEILERMSQIFDQAHGGAGPSNAALGTRLLPTLQENVGQLLLVEGSEYLMWNTFDVHFYS 546
V +LE+M+ ++ H SN+A GT+LL +EN+G L +EG EY MWNT+DVHFY+
Sbjct: 491 VSVLEKMASTLEELHAST-TSNSAFGTKLLEEGEENIGHFLYLEGIEYRMWNTYDVHFYA 550
Query: 547 SFALIMLFPKLELGIQRDFAAAVLMHDPRKAKIMSDGKYYPRKVIGAVPHDIGFNDPWFE 606
SFAL+MLFPKLEL IQRDFAAAV++HDP K K +S+G++ RKV+GAVPHD+G NDPWFE
Sbjct: 551 SFALVMLFPKLELSIQRDFAAAVMLHDPTKVKTLSEGQWVQRKVLGAVPHDLGINDPWFE 610
Query: 607 VNAYNLLNVTRWKDLGSKFVLQVYRDVVATGDKNFAKSVWPSVYVALAFMDQFDKDKDGM 666
VN Y L N RWKDL KFVLQVYRDVVATGDK FA +VWPSVYVA+A+M QFDKD DGM
Sbjct: 611 VNGYTLHNTDRWKDLNPKFVLQVYRDVVATGDKKFASAVWPSVYVAMAYMAQFDKDGDGM 670
Query: 667 IENEGFPDQTYDVWTVKGVSAYCGGLWVAALQAASALAYEVDDEAAAHYFWIKYQKARGV 726
IENEGFPDQTYD W+ GVSAYCGGLWVAALQAASALA V D+ + YFW K+QKA+ V
Sbjct: 671 IENEGFPDQTYDTWSASGVSAYCGGLWVAALQAASALARVVGDKNSQDYFWSKFQKAKVV 730
Query: 727 YD-TLWNGSYFNYDNSGGPWSSSIQADQLAGQWYARACGLCPIVDEEKIRSTLEKIFNFN 786
Y+ LWNGSYFNYDNSG +SS+IQADQLAGQWYARA GL PIVDE+K R+ LEK++N+N
Sbjct: 731 YEKKLWNGSYFNYDNSGSQYSSTIQADQLAGQWYARASGLLPIVDEDKARTALEKVYNYN 790
Query: 787 VMKVKGGTRGAVNGMFPDGRVDRSILQPKEIWAGVTYSVAASMIQEGMVETGFQTAMGIH 846
VMK+K G RGAVNGM P+G+VD + +Q +EIW+GVTY+++A+MIQEG+VE FQTA GI+
Sbjct: 791 VMKIKDGKRGAVNGMHPNGKVDTASMQSREIWSGVTYALSATMIQEGLVEMAFQTASGIY 850
Query: 847 QAAWGQDGLGYSFQTPEAWDIDDKYRSLGYMRPLAIWAMQWAISKPTLLKKHKVPRKELS 906
+AAW + GLGYSFQTPE+W+ D+YRSL YMRPLAIWAMQWA++K + ++ E
Sbjct: 851 EAAWSETGLGYSFQTPESWNTVDEYRSLTYMRPLAIWAMQWALTKTSQKQEQLGLEPEQQ 910
Query: 907 ESEESCLFSTQH-AAFLKVASLLKLPSEEAKPRSVVEVAYDFICKR 950
E E S +H F +V+ LL LP+ EA +S ++ +D+ C+R
Sbjct: 911 EPELEPSSSMKHDIGFSRVSRLLSLPN-EASAKSTLQTLFDYTCRR 954
BLAST of CmoCh01G009010 vs. TAIR 10
Match:
AT1G33700.1 (Beta-glucosidase, GBA2 type family protein )
HSP 1 Score: 1184.5 bits (3063), Expect = 0.0e+00
Identity = 581/942 (61.68%), Postives = 699/942 (74.20%), Query Frame = 0
Query: 17 MVVDPAKLPSLTWQRKLDFTGKSPESFSFTLNDKWHLGPLGYRLWRHGKEEAAKGRIPLY 76
MV+D KLP +W+RKL+ K+P F + D HL PLGYRLWRH K+EAAKGR ++
Sbjct: 11 MVIDD-KLPPFSWERKLNSQAKTPSEFKLSKRDHMHLFPLGYRLWRHTKDEAAKGRASIF 70
Query: 77 DFFASPPITCYHGVSLGGIGAGSIGRSYRGEFQRFQMFYGPSEDVPVLANQFSAFVSRPN 136
D F IT HGV LGGIG+GSIGRSY+GEFQ+F++F E+ P+L NQFSAFVSRP
Sbjct: 71 DIFRKHHITGDHGVPLGGIGSGSIGRSYKGEFQQFKLFPKICEEAPILTNQFSAFVSRPG 130
Query: 137 GNKFSTVLCSARPQKSKD---------GKNDGIEAWDWNLSGEKSTYHALFPRAWTIYDG 196
G K STVLC ++PQ KD N GIE+WDWN++GEKSTYHAL+PR+WT+YDG
Sbjct: 131 GVKHSTVLCPSKPQVVKDNGGYLCQGQAPNMGIESWDWNMTGEKSTYHALYPRSWTVYDG 190
Query: 197 EPDPDLKIVCRQISPIIPHNYKESSFPVSVFTFNLSNKGPTSAEVTLLFTWANSVGGNSG 256
EPDP+L+IV RQ+SP IPHNY+ESS PVSVF F ++N G A VTLLFTW NSVGG SG
Sbjct: 191 EPDPELRIVSRQVSPFIPHNYEESSLPVSVFNFTVTNTGAEPAIVTLLFTWENSVGGASG 250
Query: 257 FTGHHFNSKIGAEDGAHGVLLHHKTANGRPTVTYAITAEATDDVHVSVCPRFVISGDSEG 316
TG HFNS + A+DG H V L HKTANG P V+YAI A+ T+DV VS CP F++SG +
Sbjct: 251 LTGQHFNSTMKAKDGVHAVALQHKTANGHPPVSYAIAAKETEDVRVSSCPCFIVSGTTPN 310
Query: 317 -ISAKDMWQEVKKHGSFDELGSSGPHEGSKPGSSIGAAIAAKVSIPSSSTRTVTFSLAWD 376
I+A DMW E+KK+ SFD+L +S SKPG+SIGAAIAAKV +P RTVTFSL+WD
Sbjct: 311 QITAGDMWDEIKKNASFDKL-TSNACSPSKPGTSIGAAIAAKVKVPPGCDRTVTFSLSWD 370
Query: 377 CPEVKFDGKTYHRRYTNFYGTLGEAAKTIARDAILEHGKWEGQIEAWQRPIVEDKRLPEW 436
CPE +FD KTYHRRYT FYG+LG AA +A DA+L +WE QIE WQ P++ D LPEW
Sbjct: 371 CPEARFDEKTYHRRYTRFYGSLGNAAVAMAHDALLNFPEWETQIEEWQAPVLADTTLPEW 430
Query: 437 YPVTLFNELYFLNSGGTMWTDGLPPLQNLSTISHQKIFLERTKSEPNGAPNGDHRLDVAV 496
Y VTLFNELY+ NSGGTMWTDGLPP Q+L +I +KI L + + N +VA+
Sbjct: 431 YRVTLFNELYYFNSGGTMWTDGLPPKQSLDSIGRRKISLGLSTIDKNDQDQN----NVAL 490
Query: 497 EILERMSQIFDQAHGGAGPSNAALGTRLLPTLQENVGQLLLVEGSEYLMWNTFDVHFYSS 556
+IL R+ + Q H SNAALG ++ EN+GQ L +EG +YLM+NT+DVHFYSS
Sbjct: 491 DILGRIDAVCSQIHAPL-TSNAALGNTMIQNTTENIGQFLYLEGVQYLMYNTYDVHFYSS 550
Query: 557 FALIMLFPKLELGIQRDFAAAVLMHDPRKAKIMSDGKYYPRKVIGAVPHDIGFNDPWFEV 616
FAL+MLFPK+EL IQRDFAAAVLMHD K ++MS G++ RKV+GAVPHDIG NDPWFE+
Sbjct: 551 FALLMLFPKIELSIQRDFAAAVLMHDSSKKQVMSSGEFVTRKVLGAVPHDIGLNDPWFEL 610
Query: 617 NAYNLLNVTRWKDLGSKFVLQVYRDVVATGDKNFAKSVWPSVYVALAFMDQFDKDKDGMI 676
NAYNL N RWKDL SKFVLQVYRDVVATGD NFAK+VWPSVY A+A++DQFDKD DGMI
Sbjct: 611 NAYNLFNTDRWKDLNSKFVLQVYRDVVATGDLNFAKAVWPSVYTAIAYLDQFDKDGDGMI 670
Query: 677 ENEGFPDQTYDVWTVKGVSAYCGGLWVAALQAASALAYEVDDEAAAHYFWIKYQKARGVY 736
ENEGFPDQTYD W+ GVSAYCGGLWVAALQA SALA E+ D AA YF KY+KAR VY
Sbjct: 671 ENEGFPDQTYDAWSCSGVSAYCGGLWVAALQAGSALAREIGDNGAAVYFNAKYEKARSVY 730
Query: 737 DTLWNGSYFNYDNSGGPWSSSIQADQLAGQWYARACGLCPIVDEEKIRSTLEKIFNFNVM 796
+ LWNGSYFNYDNS SSSI ADQ+AGQWYARACGL PI EE I+ LE +++FNVM
Sbjct: 731 EKLWNGSYFNYDNSRSGSSSSILADQMAGQWYARACGLKPIAKEEWIKKALETVYDFNVM 790
Query: 797 KVKGGTRGAVNGMFPDGRVDRSILQPKEIWAGVTYSVAASMIQEGMVETGFQTAMGIHQA 856
+V+ GTRGAVNGM PDGRVD S + +E+WAG TYSVAA MIQEG+ + GF+TA GI++A
Sbjct: 791 RVRDGTRGAVNGMLPDGRVDTSTMVSREVWAGTTYSVAACMIQEGLADKGFRTASGIYEA 850
Query: 857 AWGQDGLGYSFQTPEAWDIDDKYRSLGYMRPLAIWAMQWAISKPTLLKKHKVPRKELSES 916
AW GLG +FQTPEAW +D+YRSL YMRPLAIW +QWA + P ++ +V + E
Sbjct: 851 AWSDRGLGCAFQTPEAWTTNDEYRSLCYMRPLAIWGIQWAHTMPKPNREQEVSLRPQEED 910
Query: 917 EESCLFSTQHAAFLKVASLLKLPSEEAKPRSVVEVAYDFICK 949
S LF QHA F+KVA LK ++ R+ ++ AY+ K
Sbjct: 911 ATSVLFQ-QHAGFIKVAHYLK-NTKGKDHRNRLQTAYETFLK 943
BLAST of CmoCh01G009010 vs. TAIR 10
Match:
AT1G33700.2 (Beta-glucosidase, GBA2 type family protein )
HSP 1 Score: 1184.5 bits (3063), Expect = 0.0e+00
Identity = 581/942 (61.68%), Postives = 699/942 (74.20%), Query Frame = 0
Query: 17 MVVDPAKLPSLTWQRKLDFTGKSPESFSFTLNDKWHLGPLGYRLWRHGKEEAAKGRIPLY 76
MV+D KLP +W+RKL+ K+P F + D HL PLGYRLWRH K+EAAKGR ++
Sbjct: 11 MVIDD-KLPPFSWERKLNSQAKTPSEFKLSKRDHMHLFPLGYRLWRHTKDEAAKGRASIF 70
Query: 77 DFFASPPITCYHGVSLGGIGAGSIGRSYRGEFQRFQMFYGPSEDVPVLANQFSAFVSRPN 136
D F IT HGV LGGIG+GSIGRSY+GEFQ+F++F E+ P+L NQFSAFVSRP
Sbjct: 71 DIFRKHHITGDHGVPLGGIGSGSIGRSYKGEFQQFKLFPKICEEAPILTNQFSAFVSRPG 130
Query: 137 GNKFSTVLCSARPQKSKD---------GKNDGIEAWDWNLSGEKSTYHALFPRAWTIYDG 196
G K STVLC ++PQ KD N GIE+WDWN++GEKSTYHAL+PR+WT+YDG
Sbjct: 131 GVKHSTVLCPSKPQVVKDNGGYLCQGQAPNMGIESWDWNMTGEKSTYHALYPRSWTVYDG 190
Query: 197 EPDPDLKIVCRQISPIIPHNYKESSFPVSVFTFNLSNKGPTSAEVTLLFTWANSVGGNSG 256
EPDP+L+IV RQ+SP IPHNY+ESS PVSVF F ++N G A VTLLFTW NSVGG SG
Sbjct: 191 EPDPELRIVSRQVSPFIPHNYEESSLPVSVFNFTVTNTGAEPAIVTLLFTWENSVGGASG 250
Query: 257 FTGHHFNSKIGAEDGAHGVLLHHKTANGRPTVTYAITAEATDDVHVSVCPRFVISGDSEG 316
TG HFNS + A+DG H V L HKTANG P V+YAI A+ T+DV VS CP F++SG +
Sbjct: 251 LTGQHFNSTMKAKDGVHAVALQHKTANGHPPVSYAIAAKETEDVRVSSCPCFIVSGTTPN 310
Query: 317 -ISAKDMWQEVKKHGSFDELGSSGPHEGSKPGSSIGAAIAAKVSIPSSSTRTVTFSLAWD 376
I+A DMW E+KK+ SFD+L +S SKPG+SIGAAIAAKV +P RTVTFSL+WD
Sbjct: 311 QITAGDMWDEIKKNASFDKL-TSNACSPSKPGTSIGAAIAAKVKVPPGCDRTVTFSLSWD 370
Query: 377 CPEVKFDGKTYHRRYTNFYGTLGEAAKTIARDAILEHGKWEGQIEAWQRPIVEDKRLPEW 436
CPE +FD KTYHRRYT FYG+LG AA +A DA+L +WE QIE WQ P++ D LPEW
Sbjct: 371 CPEARFDEKTYHRRYTRFYGSLGNAAVAMAHDALLNFPEWETQIEEWQAPVLADTTLPEW 430
Query: 437 YPVTLFNELYFLNSGGTMWTDGLPPLQNLSTISHQKIFLERTKSEPNGAPNGDHRLDVAV 496
Y VTLFNELY+ NSGGTMWTDGLPP Q+L +I +KI L + + N +VA+
Sbjct: 431 YRVTLFNELYYFNSGGTMWTDGLPPKQSLDSIGRRKISLGLSTIDKNDQDQN----NVAL 490
Query: 497 EILERMSQIFDQAHGGAGPSNAALGTRLLPTLQENVGQLLLVEGSEYLMWNTFDVHFYSS 556
+IL R+ + Q H SNAALG ++ EN+GQ L +EG +YLM+NT+DVHFYSS
Sbjct: 491 DILGRIDAVCSQIHAPL-TSNAALGNTMIQNTTENIGQFLYLEGVQYLMYNTYDVHFYSS 550
Query: 557 FALIMLFPKLELGIQRDFAAAVLMHDPRKAKIMSDGKYYPRKVIGAVPHDIGFNDPWFEV 616
FAL+MLFPK+EL IQRDFAAAVLMHD K ++MS G++ RKV+GAVPHDIG NDPWFE+
Sbjct: 551 FALLMLFPKIELSIQRDFAAAVLMHDSSKKQVMSSGEFVTRKVLGAVPHDIGLNDPWFEL 610
Query: 617 NAYNLLNVTRWKDLGSKFVLQVYRDVVATGDKNFAKSVWPSVYVALAFMDQFDKDKDGMI 676
NAYNL N RWKDL SKFVLQVYRDVVATGD NFAK+VWPSVY A+A++DQFDKD DGMI
Sbjct: 611 NAYNLFNTDRWKDLNSKFVLQVYRDVVATGDLNFAKAVWPSVYTAIAYLDQFDKDGDGMI 670
Query: 677 ENEGFPDQTYDVWTVKGVSAYCGGLWVAALQAASALAYEVDDEAAAHYFWIKYQKARGVY 736
ENEGFPDQTYD W+ GVSAYCGGLWVAALQA SALA E+ D AA YF KY+KAR VY
Sbjct: 671 ENEGFPDQTYDAWSCSGVSAYCGGLWVAALQAGSALAREIGDNGAAVYFNAKYEKARSVY 730
Query: 737 DTLWNGSYFNYDNSGGPWSSSIQADQLAGQWYARACGLCPIVDEEKIRSTLEKIFNFNVM 796
+ LWNGSYFNYDNS SSSI ADQ+AGQWYARACGL PI EE I+ LE +++FNVM
Sbjct: 731 EKLWNGSYFNYDNSRSGSSSSILADQMAGQWYARACGLKPIAKEEWIKKALETVYDFNVM 790
Query: 797 KVKGGTRGAVNGMFPDGRVDRSILQPKEIWAGVTYSVAASMIQEGMVETGFQTAMGIHQA 856
+V+ GTRGAVNGM PDGRVD S + +E+WAG TYSVAA MIQEG+ + GF+TA GI++A
Sbjct: 791 RVRDGTRGAVNGMLPDGRVDTSTMVSREVWAGTTYSVAACMIQEGLADKGFRTASGIYEA 850
Query: 857 AWGQDGLGYSFQTPEAWDIDDKYRSLGYMRPLAIWAMQWAISKPTLLKKHKVPRKELSES 916
AW GLG +FQTPEAW +D+YRSL YMRPLAIW +QWA + P ++ +V + E
Sbjct: 851 AWSDRGLGCAFQTPEAWTTNDEYRSLCYMRPLAIWGIQWAHTMPKPNREQEVSLRPQEED 910
Query: 917 EESCLFSTQHAAFLKVASLLKLPSEEAKPRSVVEVAYDFICK 949
S LF QHA F+KVA LK ++ R+ ++ AY+ K
Sbjct: 911 ATSVLFQ-QHAGFIKVAHYLK-NTKGKDHRNRLQTAYETFLK 943
BLAST of CmoCh01G009010 vs. TAIR 10
Match:
AT4G10060.1 (Beta-glucosidase, GBA2 type family protein )
HSP 1 Score: 1136.3 bits (2938), Expect = 0.0e+00
Identity = 557/935 (59.57%), Postives = 680/935 (72.73%), Query Frame = 0
Query: 16 TMVVDPAKLPSLTWQRKLDFTGKSPESFSFTLNDKWHLGPLGYRLWRHGKEEAAKGRIPL 75
T +V +LP +TWQRKL+ K+P F ++ D HL PLGYRLWR+ KEEA KGR +
Sbjct: 13 TQMVGNERLPQVTWQRKLNSKVKNPSEFKMSIRDVLHLFPLGYRLWRYTKEEAKKGRFSM 72
Query: 76 YDFFASPPITCYHGVSLGGIGAGSIGRSYRGEFQRFQMFYGPSEDVPVLANQFSAFVSRP 135
YD F + HGV LGGIG GSIGRSY+GEFQ+F++F E+ P+L NQFS FVSRP
Sbjct: 73 YDIFKKRHVRGDHGVPLGGIGGGSIGRSYKGEFQQFKLFPKICEEAPILTNQFSVFVSRP 132
Query: 136 NGNKFSTVLCSARPQKSKDGKND-GIEAWDWNLSGEKSTYHALFPRAWTIYDGEPDPDLK 195
G +STVLC +P+ K D GIE+WDWN+ G+KSTYHAL+PR+WT+Y+ EPDP+L+
Sbjct: 133 GGLSYSTVLCPTKPKSVKGKTEDLGIESWDWNMEGDKSTYHALYPRSWTVYN-EPDPELR 192
Query: 196 IVCRQISPIIPHNYKESSFPVSVFTFNLSNKGPTSAEVTLLFTWANSVGGNSGFTGHHFN 255
IV RQ+SP IPHNYKESS PVSVF F +SN G A VTLLFTW NSVGG SG TG HFN
Sbjct: 193 IVSRQVSPFIPHNYKESSLPVSVFEFTMSNLGKEEATVTLLFTWENSVGGASGLTGEHFN 252
Query: 256 SKIGAEDGAHGVLLHHKTANGRPTVTYAITAEATDDVHVSVCPRFVISGDS-EGISAKDM 315
S I DG H ++LHHKT NG P VTYAI A+ T+DVHVS CP F++SG S + I+AK+M
Sbjct: 253 STIMERDGVHAIVLHHKTGNGHPPVTYAIAAQETEDVHVSECPCFLVSGHSPKEITAKEM 312
Query: 316 WQEVKKHGSFDELGSSGPHEGSKPGSSIGAAIAAKVSIPSSSTRTVTFSLAWDCPEVKFD 375
W E+KK+ SFDEL S P S+PG+SIGAAIAAKV +P RTVTFSL+WDCPEV+F+
Sbjct: 313 WDEIKKNKSFDELNSE-PGSPSRPGTSIGAAIAAKVKVPPGCDRTVTFSLSWDCPEVRFN 372
Query: 376 GKTYHRRYTNFYGTLGEAAKTIARDAILEHGKWEGQIEAWQRPIVEDKRLPEWYPVTLFN 435
KTYHRRYT FYG LG+AA +ARDA+L + WE QIEAWQ PI+ D LP+WY VTLFN
Sbjct: 373 EKTYHRRYTKFYGNLGDAAVKMARDALLNYVDWESQIEAWQSPILSDTTLPDWYRVTLFN 432
Query: 436 ELYFLNSGGTMWTDGLPPLQNLSTISHQKIFLERTKSEPNGAPNGDHRLDVAVEILERMS 495
ELY+ NSGGT+WTDGLPP + +I K+ T +E N D+ +++ ++++
Sbjct: 433 ELYYFNSGGTIWTDGLPPKE---SIERSKV----TNTEQN---------DIVIDLFQKIN 492
Query: 496 QIFDQAHGGAGPSNAALGTRLLPTLQENVGQLLLVEGSEYLMWNTFDVHFYSSFALIMLF 555
+ +Q + ++ +EN+GQ + +EG EYLM+NT+DVHFYSSFAL+ LF
Sbjct: 493 AVCEQIYSPQSSNS-----------EENIGQFIYLEGIEYLMYNTYDVHFYSSFALLSLF 552
Query: 556 PKLELGIQRDFAAAVLMHDPRKAKIMSDGKYYPRKVIGAVPHDIGFNDPWFEVNAYNLLN 615
PKL L IQRDFAA VL+ DP K KIMS G++ RK++G+VPHDIG NDPW E+N YN N
Sbjct: 553 PKLALSIQRDFAATVLIQDPTKKKIMSSGEWVTRKLLGSVPHDIGLNDPWLELNEYNFFN 612
Query: 616 VTRWKDLGSKFVLQVYRDVVATGDKNFAKSVWPSVYVALAFMDQFDKDKDGMIENEGFPD 675
RWKDL +KFVLQVYRDVVAT D++FAK+VWPSVY A+A++DQFDKD+DGMIENEGFPD
Sbjct: 613 TDRWKDLNAKFVLQVYRDVVATNDQSFAKAVWPSVYTAVAYLDQFDKDEDGMIENEGFPD 672
Query: 676 QTYDVWTVKGVSAYCGGLWVAALQAASALAYEVDDEAAAHYFWIKYQKARGVYDTLWNGS 735
QTYD W+V GVSAYCGGLWVAALQAASA A V + A A YF KY+KA+ VY+ LWNGS
Sbjct: 673 QTYDAWSVTGVSAYCGGLWVAALQAASAFASIVGENAVAIYFNAKYEKAKIVYEKLWNGS 732
Query: 736 YFNYDNSGGPWSSSIQADQLAGQWYARACGLCPIVDEEKIRSTLEKIFNFNVMKVKGGTR 795
YFNYD+SG SSSI ADQLAGQWYARACGL PI EE I+ LE I+ FNVMKVKGGTR
Sbjct: 733 YFNYDDSGSGSSSSILADQLAGQWYARACGLKPITKEEWIKKALETIYEFNVMKVKGGTR 792
Query: 796 GAVNGMFPDGRVDRSILQPKEIWAGVTYSVAASMIQEGMVETGFQTAMGIHQAAWGQDGL 855
GAVNGM +G+VD + L KE+WAG TYSVAA MIQEG E GFQTA GI++A W GL
Sbjct: 793 GAVNGMSTEGKVDTNSLVSKEVWAGTTYSVAACMIQEGQREKGFQTASGIYEAIWSDRGL 852
Query: 856 GYSFQTPEAWDIDDKYRSLGYMRPLAIWAMQWAISKPTLLKKHKVPRKELSESEESCLFS 915
SFQTPEAW+++D+YRSL YMRPLAIWA+QWA+++ + K E EES L
Sbjct: 853 SCSFQTPEAWNMNDEYRSLCYMRPLAIWAIQWALTRTQSFGEEKQKLVAGDEEEESNLLL 912
Query: 916 TQHAAFLKVASLLKLPSEEAKPRSVVEVAYDFICK 949
QH F VA +K+ RS ++ Y+ + K
Sbjct: 913 RQHKGFKDVARFVKIVPTSNVHRSRLQHTYETVLK 918
BLAST of CmoCh01G009010 vs. TAIR 10
Match:
AT3G24180.1 (Beta-glucosidase, GBA2 type family protein )
HSP 1 Score: 888.3 bits (2294), Expect = 5.6e-258
Identity = 451/883 (51.08%), Postives = 583/883 (66.02%), Query Frame = 0
Query: 20 DPAKLPSLTWQRKLDFTGKSPESFSFTLNDKWHLGPLGYRLWRHGKEEAAKGRIPLYDFF 79
D A P W+R+L+ + F+ T + + LG RLW + +EEA+ GR D F
Sbjct: 31 DSAAPPPHAWRRRLNCHANILKEFTITFREAIKMVRLGIRLWSYVREEASHGRKAPIDPF 90
Query: 80 ASPPI--TCYHGVSLGGIGAGSIGRSYRGEFQRFQMFYGPSEDVPVLANQFSAFVSRPNG 139
+ GV LGG+G+GSI R +RGEF+++Q+ G + P+++NQFS F+SR G
Sbjct: 91 TKENCKPSASQGVPLGGMGSGSISRGFRGEFKQWQITPGTCDPSPMMSNQFSIFISRDGG 150
Query: 140 N-KFSTVLCSARPQKSKDGKNDGIEAWDWNLSGEKSTYHALFPRAWTIYDGEPDPDLKIV 199
+ K+++VL + ++ G+ +W WNL+G+ STYHALFPRAWTIYDGEPDP+LKI
Sbjct: 151 HKKYASVLSPGQHGSLGKSRDKGLSSWGWNLNGQHSTYHALFPRAWTIYDGEPDPELKIS 210
Query: 200 CRQISPIIPHNYKESSFPVSVFTFNLSNKGPTSAEVTLLFTWANSVGGNSGFTGHHFNSK 259
CRQISP IP+NY++SS P +VF + L N G A+V+LLFTWANS+GG S +G H N
Sbjct: 211 CRQISPFIPNNYRDSSLPAAVFVYTLVNTGKERAKVSLLFTWANSMGGTSHMSGGHVNEP 270
Query: 260 IGAEDGAHGVLLHHKTANGRPTVTYAITAEATDDVHVSVCPRFVISGDSEGISAKDMWQE 319
EDG GVLLHHKT G P VT+AI A T +V+V+V P F +S DS +AKDMW
Sbjct: 271 FIGEDGVSGVLLHHKTGKGNPPVTFAIAASETQNVNVTVLPCFGLSEDS-SFTAKDMWDT 330
Query: 320 VKKHGSFD-ELGSSGPHEGSKPGSSIGAAIAAKVSIPSSSTRTVTFSLAWDCPEVKFD-G 379
+++ G FD E +SGP S G +I AA++A + + TV+F+L+W P+VKF G
Sbjct: 331 MEQDGKFDQENFNSGPSTPSLAGDTICAAVSASAWVEAHGKCTVSFALSWSSPKVKFSKG 390
Query: 380 KTYHRRYTNFYGTLGEAAKTIARDAILEHGKWEGQIEAWQRPIVEDKRLPEWYPVTLFNE 439
TY RRYT FYGT AA + DA+ + +WE IEAWQ PI+ D+RLPEWY TLFNE
Sbjct: 391 STYDRRYTKFYGTSPRAALDLVHDALTNYKRWEEDIEAWQNPILRDERLPEWYKFTLFNE 450
Query: 440 LYFLNSGGTMWTDGLPPLQNLSTISHQKIFLERTKSEPNGAPNGDHRLD------VAVEI 499
LYFL +GGT+W D L HQ+ L + + G D R D V V+
Sbjct: 451 LYFLVAGGTVWIDS-SSLNANGNSQHQQSGLGNSDGKVGGLDINDQRNDLGNGNSVGVKS 510
Query: 500 LERMSQIFDQAHGGAGPSNAALGTRLLPTLQENVGQLLLVEGSEYLMWNTFDVHFYSSFA 559
+ +S I ++ + G ++VG+ L +EG EY+MW T+DVHFY+S+A
Sbjct: 511 NDEVSAIHNRNGLFVDTPHVDDG--------DDVGRFLYLEGVEYVMWCTYDVHFYASYA 570
Query: 560 LIMLFPKLELGIQRDFAAAVLMHDPRKAKIMSDGKYYPRKVIGAVPHDIGFNDPWFEVNA 619
L+MLFPK+EL IQRDFA AVL D RK K +++G RKV GAVPHD+G +DPW E+NA
Sbjct: 571 LLMLFPKIELNIQRDFAKAVLSEDGRKVKFLAEGNVGIRKVRGAVPHDLGMHDPWNEMNA 630
Query: 620 YNLLNVTRWKDLGSKFVLQVYRDVVATGDKNFAKSVWPSVYVALAFMDQFDKDKDGMIEN 679
YN+ + +RWKDL KFVLQVYRD ATGD F VWP+V A+ +M+QFD+D D +IEN
Sbjct: 631 YNIHDTSRWKDLNPKFVLQVYRDFAATGDYQFGIDVWPAVRAAMEYMEQFDRDNDDLIEN 690
Query: 680 EGFPDQTYDVWTVKGVSAYCGGLWVAALQAASALAYEVDDEAAAHYFWIKYQKARGVYDT 739
+GFPDQTYD WTV GVSAYCG LW+AALQAA+A+A ++ D+ A K+ A+ +T
Sbjct: 691 DGFPDQTYDTWTVHGVSAYCGCLWLAALQAAAAMALQIGDKFFAELCKNKFLNAKAALET 750
Query: 740 -LWNGSYFNYDNSGGPWSSSIQADQLAGQWYARACGLCPIVDEEKIRSTLEKIFNFNVMK 799
LWNGSYFNYD+ S SIQ DQLAGQWYA + GL P+ +E KIRST++KIF+FNVMK
Sbjct: 751 KLWNGSYFNYDSGSSSNSKSIQTDQLAGQWYAASSGLPPLFEESKIRSTMQKIFDFNVMK 810
Query: 800 VKGGTRGAVNGMFPDGRVDRSILQPKEIWAGVTYSVAASMIQEGMVETGFQTAMGIHQAA 859
KGG GAVNGM PDG+VD + +Q +EIW GVTY+ AA+MI GM E GF TA GI A
Sbjct: 811 TKGGKMGAVNGMHPDGKVDDTCMQSREIWTGVTYAAAATMILSGMEEQGFTTAEGIFTAG 870
Query: 860 WGQDGLGYSFQTPEAWDIDDKYRSLGYMRPLAIWAMQWAISKP 891
W ++G GY FQTPE W +D YRSL YMRPLAIW MQWA+S P
Sbjct: 871 WSEEGFGYWFQTPEGWTMDGHYRSLIYMRPLAIWGMQWALSLP 903
The following BLAST results are available for this feature:
Match Name | E-value | Identity | Description | |
Q69ZF3 | 7.8e-164 | 37.78 | Non-lysosomal glucosylceramidase OS=Mus musculus OX=10090 GN=Gba2 PE=1 SV=2 | [more] |
Q9HCG7 | 1.5e-162 | 38.69 | Non-lysosomal glucosylceramidase OS=Homo sapiens OX=9606 GN=GBA2 PE=1 SV=2 | [more] |
Q5M868 | 5.6e-162 | 38.32 | Non-lysosomal glucosylceramidase OS=Rattus norvegicus OX=10116 GN=Gba2 PE=2 SV=2 | [more] |
Q7KT91 | 6.2e-137 | 33.58 | Non-lysosomal glucosylceramidase OS=Drosophila melanogaster OX=7227 GN=CG33090 P... | [more] |
Match Name | E-value | Identity | Description | |
A0A6J1GAM9 | 0.0e+00 | 100.00 | Non-lysosomal glucosylceramidase OS=Cucurbita moschata OX=3662 GN=LOC111452445 P... | [more] |
A0A6J1GBB8 | 0.0e+00 | 99.17 | Non-lysosomal glucosylceramidase OS=Cucurbita moschata OX=3662 GN=LOC111452445 P... | [more] |
A0A6J1GAL9 | 0.0e+00 | 99.06 | Non-lysosomal glucosylceramidase OS=Cucurbita moschata OX=3662 GN=LOC111452445 P... | [more] |
A0A6J1I8G5 | 0.0e+00 | 98.42 | Non-lysosomal glucosylceramidase OS=Cucurbita maxima OX=3661 GN=LOC111471931 PE=... | [more] |
A0A6J1IED1 | 0.0e+00 | 97.60 | Non-lysosomal glucosylceramidase OS=Cucurbita maxima OX=3661 GN=LOC111471931 PE=... | [more] |
Match Name | E-value | Identity | Description | |
XP_022948947.1 | 0.0e+00 | 100.00 | non-lysosomal glucosylceramidase-like isoform X3 [Cucurbita moschata] | [more] |
KAG6607519.1 | 0.0e+00 | 99.79 | Non-lysosomal glucosylceramidase, partial [Cucurbita argyrosperma subsp. sororia... | [more] |
XP_022948945.1 | 0.0e+00 | 99.17 | non-lysosomal glucosylceramidase-like isoform X1 [Cucurbita moschata] | [more] |
XP_023524726.1 | 0.0e+00 | 99.37 | non-lysosomal glucosylceramidase-like isoform X4 [Cucurbita pepo subsp. pepo] | [more] |
XP_022948946.1 | 0.0e+00 | 99.06 | non-lysosomal glucosylceramidase-like isoform X2 [Cucurbita moschata] | [more] |