CmoCh01G008870 (gene) Cucurbita moschata (Rifu) v1

Overview


Name	CmoCh01G008870
Type	gene
Organism	Cucurbita moschata (Cucurbita moschata (Rifu) v1)
Description	pectinesterase inhibitor-like
Location	Cmo_Chr01: 4841121 .. 4841651 (-)
RNA-Seq Expression	CmoCh01G008870
Synteny	CmoCh01G008870

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGACATCTTCTAGTTTTCTTGCAATTGTCCTTTTAGTTCTGTTCTTCAATGGGATGGTGCCCATGCATGCAGCTTCCCAAAACGATATCGTTTCCACCATCTGCAAGAAAACTAGAAACCCTTCTTTTTGCTTTAACGTGTTGAAATCTGCTGGCACCACCGACCTAAAAGGGCTGGCCACCTTCACCCTTAACCTCGCCCACGACAAGGCTACTCAAAGTTATGCCCTCGCCCAGTCCCTGGCGTCCAAGGCAGTCGATCCCAAGCTTAAGGAGCGCTATGCCACTTGTGCTGAACTCTACGATGATGCTGTCGACGACATCGAGGATGGGAAGAACTACTTGGGGGAAGGTGACTACAATGGCGTCAACATTAAAGCGTCTGCAGCCATGACAGAGGCCAACGACTGTCTAGATAGCTTCACGCAGCCGCCGAAGGACCCATCGGCGCTGTCTGGCAACGGAAAGACTGTGGAAGATATTTGTAGTATCATCTTGGTTATAGCCAATCTTCTTCTTGGGCATGCCTAA

mRNA sequence

ATGACATCTTCTAGTTTTCTTGCAATTGTCCTTTTAGTTCTGTTCTTCAATGGGATGGTGCCCATGCATGCAGCTTCCCAAAACGATATCGTTTCCACCATCTGCAAGAAAACTAGAAACCCTTCTTTTTGCTTTAACGTGTTGAAATCTGCTGGCACCACCGACCTAAAAGGGCTGGCCACCTTCACCCTTAACCTCGCCCACGACAAGGCTACTCAAAGTTATGCCCTCGCCCAGTCCCTGGCGTCCAAGGCAGTCGATCCCAAGCTTAAGGAGCGCTATGCCACTTGTGCTGAACTCTACGATGATGCTGTCGACGACATCGAGGATGGGAAGAACTACTTGGGGGAAGGTGACTACAATGGCGTCAACATTAAAGCGTCTGCAGCCATGACAGAGGCCAACGACTGTCTAGATAGCTTCACGCAGCCGCCGAAGGACCCATCGGCGCTGTCTGGCAACGGAAAGACTGTGGAAGATATTTGTAGTATCATCTTGGTTATAGCCAATCTTCTTCTTGGGCATGCCTAA

Coding sequence (CDS)

ATGACATCTTCTAGTTTTCTTGCAATTGTCCTTTTAGTTCTGTTCTTCAATGGGATGGTGCCCATGCATGCAGCTTCCCAAAACGATATCGTTTCCACCATCTGCAAGAAAACTAGAAACCCTTCTTTTTGCTTTAACGTGTTGAAATCTGCTGGCACCACCGACCTAAAAGGGCTGGCCACCTTCACCCTTAACCTCGCCCACGACAAGGCTACTCAAAGTTATGCCCTCGCCCAGTCCCTGGCGTCCAAGGCAGTCGATCCCAAGCTTAAGGAGCGCTATGCCACTTGTGCTGAACTCTACGATGATGCTGTCGACGACATCGAGGATGGGAAGAACTACTTGGGGGAAGGTGACTACAATGGCGTCAACATTAAAGCGTCTGCAGCCATGACAGAGGCCAACGACTGTCTAGATAGCTTCACGCAGCCGCCGAAGGACCCATCGGCGCTGTCTGGCAACGGAAAGACTGTGGAAGATATTTGTAGTATCATCTTGGTTATAGCCAATCTTCTTCTTGGGCATGCCTAA

Protein sequence

MTSSSFLAIVLLVLFFNGMVPMHAASQNDIVSTICKKTRNPSFCFNVLKSAGTTDLKGLATFTLNLAHDKATQSYALAQSLASKAVDPKLKERYATCAELYDDAVDDIEDGKNYLGEGDYNGVNIKASAAMTEANDCLDSFTQPPKDPSALSGNGKTVEDICSIILVIANLLLGHA

Homology

[Close]

BLAST of CmoCh01G008870 vs. ExPASy Swiss-Prot
Match: P83326 (Pectinesterase inhibitor OS=Actinidia deliciosa OX=3627 GN=PMEI PE=1 SV=2)

HSP 1 Score: 142.1 bits (357), Expect = 5.9e-33
Identity = 77/177 (43.50%), Postives = 112/177 (63.28%), Query Frame = 0


Query: 2   TSSSFLAIVLLVLFFNGMVPMHAA-SQNDIVSTICKKTRNPSFCFNVLKS---AGTTDLK 61
           +SS F++++L++LF + +    +  ++N ++S IC KTRNPS C   L+S   + + DLK
Sbjct: 7   SSSLFVSLLLVILFISPLSQRPSVKAENHLISEICPKTRNPSLCLQALESDPRSASKDLK 66

Query: 62  GLATFTLNLAHDKATQSYALAQSLASKAVDPKLKERYATCAELYDDAVDDIEDGKNYLGE 121
           GL  F++++A   A Q+  +  SL ++A DPKLK RY TC+E Y DA+D +   K +L  
Sbjct: 67  GLGQFSIDIAQASAKQTSKIIASLTNQATDPKLKGRYETCSENYADAIDSLGQAKQFLTS 126

Query: 122 GDYNGVNIKASAAMTEANDCLDSFTQPPKDPSALSGNGKTVEDICSIILVIANLLLG 175
           GDYN +NI ASAA   A  C DSF  PP  P+ L      +ED+C I+LVI+NLL G
Sbjct: 127 GDYNSLNIYASAAFDGAGTCEDSFEGPPNIPTQLHQADLKLEDLCDIVLVISNLLPG 183

[Close]

BLAST of CmoCh01G008870 vs. ExPASy Swiss-Prot
Match: Q9LNF2 (Pectinesterase inhibitor 1 OS=Arabidopsis thaliana OX=3702 GN=PMEI1 PE=1 SV=1)

HSP 1 Score: 102.1 bits (253), Expect = 6.8e-21
Identity = 59/173 (34.10%), Postives = 101/173 (58.38%), Query Frame = 0


Query: 1   MTSSSFLAIVLLVLFFNGMVPMHAASQNDIVSTICKKTRNPSFCFNVLKSA-GTTDLKGL 60
           + +++FL+ ++ +L    ++    A  +  +STIC KT NPSFC   L +   + +L+ L
Sbjct: 5   LRNNAFLSSLMFLL----LIGSSYAITSSEMSTICDKTLNPSFCLKFLNTKFASPNLQAL 64

Query: 61  ATFTLNLAHDKATQSYALAQSLASKAVDPKLKERYATCAELYDDAVDDIEDGKNYLGEGD 120
           A  TL+    +ATQ+    QS+    VDP+ K  Y +C + Y+ A+ ++E+   +L  GD
Sbjct: 65  AKTTLDSTQARATQTLKKLQSIIDGGVDPRSKLAYRSCVDEYESAIGNLEEAFEHLASGD 124

Query: 121 YNGVNIKASAAMTEANDCLDSFTQPPKDPSALSGNGKTVEDICSIILVIANLL 173
             G+N+K SAA+  A+ CLD   +     S++  N KT++++C I LVI+N+L
Sbjct: 125 GMGMNMKVSAALDGADTCLDDVKRLRSVDSSVVNNSKTIKNLCGIALVISNML 173

[Close]

BLAST of CmoCh01G008870 vs. ExPASy Swiss-Prot
Match: Q9LUV1 (Pectinesterase inhibitor 2 OS=Arabidopsis thaliana OX=3702 GN=PMEI2 PE=1 SV=1)

HSP 1 Score: 95.1 bits (235), Expect = 8.3e-19
Identity = 53/145 (36.55%), Postives = 78/145 (53.79%), Query Frame = 0


Query: 31  VSTICKKTRNPSFCFNVLKSAGTT---DLKGLATFTLNLAHDKATQSYALAQSLASKAVD 90
           +  IC K +N SFC + +KS   T   DL+ LA  T   A   A++ +   QSL   A +
Sbjct: 30  IKAICGKAKNQSFCTSYMKSNPKTSGADLQTLANITFGSAQTSASEGFRKIQSLVKTATN 89

Query: 91  PKLKERYATCAELYDDAVDDIEDGKNYLGEGDYNGVNIKASAAMTEANDCLDSFTQPPKD 150
           P +K+ Y +C + Y  A+  + D K  L  GD  G+NIK SAAM   + C         D
Sbjct: 90  PTMKKAYTSCVQHYKSAISSLNDAKQSLASGDGKGLNIKVSAAMEGPSTCEQDMADFKVD 149

Query: 151 PSALSGNGKTVEDICSIILVIANLL 173
           PSA+  +G   ++IC I+LVI+N++
Sbjct: 150 PSAVKNSG-DFQNICGIVLVISNMM 173

[Close]

BLAST of CmoCh01G008870 vs. ExPASy Swiss-Prot
Match: Q9LUL8 (Putative pectinesterase/pectinesterase inhibitor 26 OS=Arabidopsis thaliana OX=3702 GN=PME26 PE=2 SV=1)

HSP 1 Score: 48.1 bits (113), Expect = 1.2e-04
Identity = 34/131 (25.95%), Postives = 57/131 (43.51%), Query Frame = 0


Query: 28  NDIVSTICKKTRNPSFCFNVLK----SAGTTDLKGLATFTLNLAHDKATQSYALAQSLAS 87
           + ++ T+C  T  P+ C + +     S  TTD K L   +L +  D+      L + LA 
Sbjct: 456 SSVLRTVCNVTNYPASCISSISKLPLSKTTTDPKVLFRLSLQVTFDELNSIVGLPKKLAE 515

Query: 88  KAVDPKLKERYATCAELYDDAVDDIED-----------GKNYLGEGDYNGVNIKASAAMT 144
           +  D  LK   + CA+++D AVD + D           GK  L       +    S+A+T
Sbjct: 516 ETNDEGLKSALSVCADVFDLAVDSVNDTISSLDEVISGGKKNLNSSTIGDLITWLSSAVT 575

[Close]

BLAST of CmoCh01G008870 vs. ExPASy TrEMBL
Match: A0A6J1GBD1 (pectinesterase inhibitor-like OS=Cucurbita moschata OX=3662 GN=LOC111452619 PE=4 SV=1)

HSP 1 Score: 314.7 bits (805), Expect = 2.5e-82
Identity = 158/158 (100.00%), Postives = 158/158 (100.00%), Query Frame = 0


Query: 19  MVPMHAASQNDIVSTICKKTRNPSFCFNVLKSAGTTDLKGLATFTLNLAHDKATQSYALA 78
           MVPMHAASQNDIVSTICKKTRNPSFCFNVLKSAGTTDLKGLATFTLNLAHDKATQSYALA
Sbjct: 1   MVPMHAASQNDIVSTICKKTRNPSFCFNVLKSAGTTDLKGLATFTLNLAHDKATQSYALA 60

Query: 79  QSLASKAVDPKLKERYATCAELYDDAVDDIEDGKNYLGEGDYNGVNIKASAAMTEANDCL 138
           QSLASKAVDPKLKERYATCAELYDDAVDDIEDGKNYLGEGDYNGVNIKASAAMTEANDCL
Sbjct: 61  QSLASKAVDPKLKERYATCAELYDDAVDDIEDGKNYLGEGDYNGVNIKASAAMTEANDCL 120

Query: 139 DSFTQPPKDPSALSGNGKTVEDICSIILVIANLLLGHA 177
           DSFTQPPKDPSALSGNGKTVEDICSIILVIANLLLGHA
Sbjct: 121 DSFTQPPKDPSALSGNGKTVEDICSIILVIANLLLGHA 158

[Close]

BLAST of CmoCh01G008870 vs. ExPASy TrEMBL
Match: A0A6J1F4D5 (pectinesterase inhibitor-like OS=Cucurbita moschata OX=3662 GN=LOC111440166 PE=4 SV=1)

HSP 1 Score: 307.4 bits (786), Expect = 3.9e-80
Identity = 161/179 (89.94%), Postives = 165/179 (92.18%), Query Frame = 0


Query: 1   MTSSSFLA---IVLLVLFFNGMVPMHAASQNDIVSTICKKTRNPSFCFNVLKSAGTTDLK 60
           M SSSFLA   IVLLVLFFNGMVPMHAASQ+DIVSTICKKTRNPSFCFNVLKSAGTTDLK
Sbjct: 1   MASSSFLAVFRIVLLVLFFNGMVPMHAASQDDIVSTICKKTRNPSFCFNVLKSAGTTDLK 60

Query: 61  GLATFTLNLAHDKATQSYALAQSLASKAVDPKLKERYATCAELYDDAVDDIEDGKNYLGE 120
           GLATFTLNLAHDK  Q++ALA SLASKA DPKLK+RYATCAE YDDA DDIEDGKN L E
Sbjct: 61  GLATFTLNLAHDKVAQTHALALSLASKAADPKLKDRYATCAEQYDDAADDIEDGKNDLRE 120

Query: 121 GDYNGVNIKASAAMTEANDCLDSFTQPPKDPSALSGNGKTVEDICSIILVIANLLLGHA 177
           GDYNGVNIKASAAMTEA DCLDSFTQPPKDPSALSGNGKTVEDICSIILVIANLLLG A
Sbjct: 121 GDYNGVNIKASAAMTEAGDCLDSFTQPPKDPSALSGNGKTVEDICSIILVIANLLLGRA 179

[Close]

BLAST of CmoCh01G008870 vs. ExPASy TrEMBL
Match: A0A6J1EZI7 (pectinesterase inhibitor-like OS=Cucurbita moschata OX=3662 GN=LOC111440672 PE=4 SV=1)

HSP 1 Score: 305.1 bits (780), Expect = 1.9e-79
Identity = 160/179 (89.39%), Postives = 162/179 (90.50%), Query Frame = 0


Query: 1   MTSSSFLA---IVLLVLFFNGMVPMHAASQNDIVSTICKKTRNPSFCFNVLKSAGTTDLK 60
           M SSSFLA   IVLLVLFFNGM PMHAASQ+DIVSTICKKTRNPSFCFNVLKSAGTTDLK
Sbjct: 1   MASSSFLAVFRIVLLVLFFNGMAPMHAASQDDIVSTICKKTRNPSFCFNVLKSAGTTDLK 60

Query: 61  GLATFTLNLAHDKATQSYALAQSLASKAVDPKLKERYATCAELYDDAVDDIEDGKNYLGE 120
           GLATFTLNLAHDK  Q+ ALA SLASK  DPKLKERYATCAE YDDA DDIEDGKN LGE
Sbjct: 61  GLATFTLNLAHDKVAQTRALALSLASKETDPKLKERYATCAEQYDDAADDIEDGKNDLGE 120

Query: 121 GDYNGVNIKASAAMTEANDCLDSFTQPPKDPSALSGNGKTVEDICSIILVIANLLLGHA 177
           GDYNGVNIKASAAMTE  DCLDSFTQPPKDPSALSGNGKTVEDICSIILVIANLLLG A
Sbjct: 121 GDYNGVNIKASAAMTEVGDCLDSFTQPPKDPSALSGNGKTVEDICSIILVIANLLLGRA 179

[Close]

BLAST of CmoCh01G008870 vs. ExPASy TrEMBL
Match: A0A6J1F469 (pectinesterase inhibitor-like OS=Cucurbita moschata OX=3662 GN=LOC111440164 PE=4 SV=1)

HSP 1 Score: 303.9 bits (777), Expect = 4.3e-79
Identity = 161/179 (89.94%), Postives = 163/179 (91.06%), Query Frame = 0


Query: 1   MTSSSFLA---IVLLVLFFNGMVPMHAASQNDIVSTICKKTRNPSFCFNVLKSAGTTDLK 60
           M SSSFLA   IVLLVLFFNGMVPMHAASQ+DIVSTICKKTRN SFCFNVLKSAGTTDLK
Sbjct: 1   MASSSFLAVFRIVLLVLFFNGMVPMHAASQDDIVSTICKKTRNSSFCFNVLKSAGTTDLK 60

Query: 61  GLATFTLNLAHDKATQSYALAQSLASKAVDPKLKERYATCAELYDDAVDDIEDGKNYLGE 120
           GLATFTLNLAHDK  Q+ ALA SLASKA DPKLKERYATCAE YDDA DDIEDGKN LGE
Sbjct: 61  GLATFTLNLAHDKVAQTRALALSLASKATDPKLKERYATCAEQYDDAADDIEDGKNDLGE 120

Query: 121 GDYNGVNIKASAAMTEANDCLDSFTQPPKDPSALSGNGKTVEDICSIILVIANLLLGHA 177
           GDYNGVNIKASAAMTEA DCLDSFTQPPKDPSALSGNGKTVEDICSIILVIANLLL  A
Sbjct: 121 GDYNGVNIKASAAMTEAGDCLDSFTQPPKDPSALSGNGKTVEDICSIILVIANLLLRRA 179

[Close]

BLAST of CmoCh01G008870 vs. ExPASy TrEMBL
Match: A0A6J1F473 (pectinesterase inhibitor-like OS=Cucurbita moschata OX=3662 GN=LOC111440170 PE=4 SV=1)

HSP 1 Score: 302.8 bits (774), Expect = 9.6e-79
Identity = 159/179 (88.83%), Postives = 162/179 (90.50%), Query Frame = 0


Query: 1   MTSSSFLA---IVLLVLFFNGMVPMHAASQNDIVSTICKKTRNPSFCFNVLKSAGTTDLK 60
           M SSSFLA   IVLLV+FFNGM PMHAASQ+DIVSTICKKTRNPSFCFNVLKSAGTTDLK
Sbjct: 1   MASSSFLAVFRIVLLVMFFNGMAPMHAASQDDIVSTICKKTRNPSFCFNVLKSAGTTDLK 60

Query: 61  GLATFTLNLAHDKATQSYALAQSLASKAVDPKLKERYATCAELYDDAVDDIEDGKNYLGE 120
           GLATFTLNLAHDK  Q+ ALA SLASKA DPKLKERYATCAE YDDA DDIEDGKN LGE
Sbjct: 61  GLATFTLNLAHDKVAQTRALALSLASKATDPKLKERYATCAEQYDDADDDIEDGKNDLGE 120

Query: 121 GDYNGVNIKASAAMTEANDCLDSFTQPPKDPSALSGNGKTVEDICSIILVIANLLLGHA 177
           GDYNGVNIKASAAMTE  DCLDSFTQPPKDPSALS NGKTVEDICSIILVIANLLLG A
Sbjct: 121 GDYNGVNIKASAAMTEVGDCLDSFTQPPKDPSALSSNGKTVEDICSIILVIANLLLGRA 179

[Close]

BLAST of CmoCh01G008870 vs. NCBI nr
Match: XP_022949197.1 (pectinesterase inhibitor-like [Cucurbita moschata])

HSP 1 Score: 314.7 bits (805), Expect = 5.1e-82
Identity = 158/158 (100.00%), Postives = 158/158 (100.00%), Query Frame = 0


Query: 19  MVPMHAASQNDIVSTICKKTRNPSFCFNVLKSAGTTDLKGLATFTLNLAHDKATQSYALA 78
           MVPMHAASQNDIVSTICKKTRNPSFCFNVLKSAGTTDLKGLATFTLNLAHDKATQSYALA
Sbjct: 1   MVPMHAASQNDIVSTICKKTRNPSFCFNVLKSAGTTDLKGLATFTLNLAHDKATQSYALA 60

Query: 79  QSLASKAVDPKLKERYATCAELYDDAVDDIEDGKNYLGEGDYNGVNIKASAAMTEANDCL 138
           QSLASKAVDPKLKERYATCAELYDDAVDDIEDGKNYLGEGDYNGVNIKASAAMTEANDCL
Sbjct: 61  QSLASKAVDPKLKERYATCAELYDDAVDDIEDGKNYLGEGDYNGVNIKASAAMTEANDCL 120

Query: 139 DSFTQPPKDPSALSGNGKTVEDICSIILVIANLLLGHA 177
           DSFTQPPKDPSALSGNGKTVEDICSIILVIANLLLGHA
Sbjct: 121 DSFTQPPKDPSALSGNGKTVEDICSIILVIANLLLGHA 158

[Close]

BLAST of CmoCh01G008870 vs. NCBI nr
Match: XP_022933240.1 (pectinesterase inhibitor-like [Cucurbita moschata])

HSP 1 Score: 307.4 bits (786), Expect = 8.1e-80
Identity = 161/179 (89.94%), Postives = 165/179 (92.18%), Query Frame = 0


Query: 1   MTSSSFLA---IVLLVLFFNGMVPMHAASQNDIVSTICKKTRNPSFCFNVLKSAGTTDLK 60
           M SSSFLA   IVLLVLFFNGMVPMHAASQ+DIVSTICKKTRNPSFCFNVLKSAGTTDLK
Sbjct: 1   MASSSFLAVFRIVLLVLFFNGMVPMHAASQDDIVSTICKKTRNPSFCFNVLKSAGTTDLK 60

Query: 61  GLATFTLNLAHDKATQSYALAQSLASKAVDPKLKERYATCAELYDDAVDDIEDGKNYLGE 120
           GLATFTLNLAHDK  Q++ALA SLASKA DPKLK+RYATCAE YDDA DDIEDGKN L E
Sbjct: 61  GLATFTLNLAHDKVAQTHALALSLASKAADPKLKDRYATCAEQYDDAADDIEDGKNDLRE 120

Query: 121 GDYNGVNIKASAAMTEANDCLDSFTQPPKDPSALSGNGKTVEDICSIILVIANLLLGHA 177
           GDYNGVNIKASAAMTEA DCLDSFTQPPKDPSALSGNGKTVEDICSIILVIANLLLG A
Sbjct: 121 GDYNGVNIKASAAMTEAGDCLDSFTQPPKDPSALSGNGKTVEDICSIILVIANLLLGRA 179

[Close]

BLAST of CmoCh01G008870 vs. NCBI nr
Match: XP_023521203.1 (pectinesterase inhibitor-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 307.4 bits (786), Expect = 8.1e-80
Identity = 162/179 (90.50%), Postives = 165/179 (92.18%), Query Frame = 0


Query: 1   MTSSSFLA---IVLLVLFFNGMVPMHAASQNDIVSTICKKTRNPSFCFNVLKSAGTTDLK 60
           M SSSFLA   IVLLVLFFNGMVPM AASQ+DIVSTICKKTRNPSFCFNVLKSAGTTDLK
Sbjct: 1   MASSSFLAVFRIVLLVLFFNGMVPMQAASQDDIVSTICKKTRNPSFCFNVLKSAGTTDLK 60

Query: 61  GLATFTLNLAHDKATQSYALAQSLASKAVDPKLKERYATCAELYDDAVDDIEDGKNYLGE 120
           GLATFTLNLAHDK  Q+ ALAQSLASKAVDPKLKERYATCAE YDDA  DIEDGKN LG+
Sbjct: 61  GLATFTLNLAHDKVAQTRALAQSLASKAVDPKLKERYATCAEQYDDAAGDIEDGKNNLGK 120

Query: 121 GDYNGVNIKASAAMTEANDCLDSFTQPPKDPSALSGNGKTVEDICSIILVIANLLLGHA 177
           GDYNGVNIKASAAMTEA DCLDSFTQPPKDPSALSGNGKTVEDICSIILVIANLLLG A
Sbjct: 121 GDYNGVNIKASAAMTEAGDCLDSFTQPPKDPSALSGNGKTVEDICSIILVIANLLLGRA 179

[Close]

BLAST of CmoCh01G008870 vs. NCBI nr
Match: XP_022933241.1 (pectinesterase inhibitor-like [Cucurbita moschata] >XP_022933352.1 pectinesterase inhibitor-like [Cucurbita moschata])

HSP 1 Score: 305.1 bits (780), Expect = 4.0e-79
Identity = 160/179 (89.39%), Postives = 162/179 (90.50%), Query Frame = 0


Query: 1   MTSSSFLA---IVLLVLFFNGMVPMHAASQNDIVSTICKKTRNPSFCFNVLKSAGTTDLK 60
           M SSSFLA   IVLLVLFFNGM PMHAASQ+DIVSTICKKTRNPSFCFNVLKSAGTTDLK
Sbjct: 1   MASSSFLAVFRIVLLVLFFNGMAPMHAASQDDIVSTICKKTRNPSFCFNVLKSAGTTDLK 60

Query: 61  GLATFTLNLAHDKATQSYALAQSLASKAVDPKLKERYATCAELYDDAVDDIEDGKNYLGE 120
           GLATFTLNLAHDK  Q+ ALA SLASK  DPKLKERYATCAE YDDA DDIEDGKN LGE
Sbjct: 61  GLATFTLNLAHDKVAQTRALALSLASKETDPKLKERYATCAEQYDDAADDIEDGKNDLGE 120

Query: 121 GDYNGVNIKASAAMTEANDCLDSFTQPPKDPSALSGNGKTVEDICSIILVIANLLLGHA 177
           GDYNGVNIKASAAMTE  DCLDSFTQPPKDPSALSGNGKTVEDICSIILVIANLLLG A
Sbjct: 121 GDYNGVNIKASAAMTEVGDCLDSFTQPPKDPSALSGNGKTVEDICSIILVIANLLLGRA 179

[Close]

BLAST of CmoCh01G008870 vs. NCBI nr
Match: XP_023522250.1 (pectinesterase inhibitor-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 304.7 bits (779), Expect = 5.2e-79
Identity = 161/179 (89.94%), Postives = 163/179 (91.06%), Query Frame = 0


Query: 1   MTSSSFLA---IVLLVLFFNGMVPMHAASQNDIVSTICKKTRNPSFCFNVLKSAGTTDLK 60
           M SSSFLA   IVLLVLFFNGMVPMHAASQ+DIVSTICKKTRNPSFCFNVLKSAGT DLK
Sbjct: 1   MASSSFLAVFRIVLLVLFFNGMVPMHAASQDDIVSTICKKTRNPSFCFNVLKSAGTRDLK 60

Query: 61  GLATFTLNLAHDKATQSYALAQSLASKAVDPKLKERYATCAELYDDAVDDIEDGKNYLGE 120
           GLATFTLNLAHDK  Q+ ALA SLASKA DPKLKERYATCAE YDDA DDIEDGKN L E
Sbjct: 61  GLATFTLNLAHDKVAQTRALALSLASKAADPKLKERYATCAEQYDDAADDIEDGKNDLRE 120

Query: 121 GDYNGVNIKASAAMTEANDCLDSFTQPPKDPSALSGNGKTVEDICSIILVIANLLLGHA 177
           GDYNGVNIKASAAMTEA DCLDSFTQPPKDPSALSGNGKTVEDICSIILVIANLLLG A
Sbjct: 121 GDYNGVNIKASAAMTEAGDCLDSFTQPPKDPSALSGNGKTVEDICSIILVIANLLLGRA 179

[Close]

BLAST of CmoCh01G008870 vs. TAIR 10
Match: AT1G48020.1 (pectin methylesterase inhibitor 1 )

HSP 1 Score: 102.1 bits (253), Expect = 4.8e-22
Identity = 59/173 (34.10%), Postives = 101/173 (58.38%), Query Frame = 0


Query: 1   MTSSSFLAIVLLVLFFNGMVPMHAASQNDIVSTICKKTRNPSFCFNVLKSA-GTTDLKGL 60
           + +++FL+ ++ +L    ++    A  +  +STIC KT NPSFC   L +   + +L+ L
Sbjct: 5   LRNNAFLSSLMFLL----LIGSSYAITSSEMSTICDKTLNPSFCLKFLNTKFASPNLQAL 64

Query: 61  ATFTLNLAHDKATQSYALAQSLASKAVDPKLKERYATCAELYDDAVDDIEDGKNYLGEGD 120
           A  TL+    +ATQ+    QS+    VDP+ K  Y +C + Y+ A+ ++E+   +L  GD
Sbjct: 65  AKTTLDSTQARATQTLKKLQSIIDGGVDPRSKLAYRSCVDEYESAIGNLEEAFEHLASGD 124

Query: 121 YNGVNIKASAAMTEANDCLDSFTQPPKDPSALSGNGKTVEDICSIILVIANLL 173
             G+N+K SAA+  A+ CLD   +     S++  N KT++++C I LVI+N+L
Sbjct: 125 GMGMNMKVSAALDGADTCLDDVKRLRSVDSSVVNNSKTIKNLCGIALVISNML 173

[Close]

BLAST of CmoCh01G008870 vs. TAIR 10
Match: AT3G17220.1 (pectin methylesterase inhibitor 2 )

HSP 1 Score: 95.1 bits (235), Expect = 5.9e-20
Identity = 53/145 (36.55%), Postives = 78/145 (53.79%), Query Frame = 0


Query: 31  VSTICKKTRNPSFCFNVLKSAGTT---DLKGLATFTLNLAHDKATQSYALAQSLASKAVD 90
           +  IC K +N SFC + +KS   T   DL+ LA  T   A   A++ +   QSL   A +
Sbjct: 30  IKAICGKAKNQSFCTSYMKSNPKTSGADLQTLANITFGSAQTSASEGFRKIQSLVKTATN 89

Query: 91  PKLKERYATCAELYDDAVDDIEDGKNYLGEGDYNGVNIKASAAMTEANDCLDSFTQPPKD 150
           P +K+ Y +C + Y  A+  + D K  L  GD  G+NIK SAAM   + C         D
Sbjct: 90  PTMKKAYTSCVQHYKSAISSLNDAKQSLASGDGKGLNIKVSAAMEGPSTCEQDMADFKVD 149

Query: 151 PSALSGNGKTVEDICSIILVIANLL 173
           PSA+  +G   ++IC I+LVI+N++
Sbjct: 150 PSAVKNSG-DFQNICGIVLVISNMM 173

[Close]

BLAST of CmoCh01G008870 vs. TAIR 10
Match: AT3G36659.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 58.2 bits (139), Expect = 8.0e-09
Identity = 46/142 (32.39%), Postives = 68/142 (47.89%), Query Frame = 0


Query: 31  VSTICKKTRNPSFCFNVLKSAGTTDLKGLATFTLNLAHDKATQSYALAQSLASKAVDPKL 90
           + TIC KT NP  C + +    T  LK   +  L LA   +  +   A ++  K VD   
Sbjct: 130 IKTICGKTDNPPLCESSVSPLLTPQLKPNTSSVLILAIQASITATKAAMAIVEK-VDA-- 189

Query: 91  KERYATCAELYDDAVDDIEDGKNYLGEGDYNGVNIKASAAMTEANDCLDSFTQPPKDPSA 150
               + C ELYDDAV ++ED  N +   D   VN   SAAMT+ + C D F +   +P+ 
Sbjct: 190 ----SDCQELYDDAVVNLEDAVNAVKSSDIATVNTNLSAAMTDYSTCNDGF-EESGEPNP 249

Query: 151 LSGNGKTVEDICSIILVIANLL 173
           L+     +  + S  L I+ L+
Sbjct: 250 LAYVADKLTKMVSNCLAISTLI 263

[Close]

BLAST of CmoCh01G008870 vs. TAIR 10
Match: AT1G55770.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 50.4 bits (119), Expect = 1.7e-06
Identity = 41/174 (23.56%), Postives = 84/174 (48.28%), Query Frame = 0


Query: 6   FLAIVLLVLFFNGMVPMHAASQNDIVSTICKKTRNPSFCFNVLKS-------AGTTDLKG 65
           FL IV L+ F  G       + + ++S +CK + +P+ C + + S       AGT++   
Sbjct: 9   FLCIV-LISFVTG------NANSGMISDLCKHSDDPNLCLSSITSRPESGEFAGTSNQIE 68

Query: 66  LATFTLNLAHDKATQSYALAQSLASKAVDPKLKERYATCAELYDDAVDDIEDGKNYLGEG 125
           +   +   A+  AT SY + Q L+++ ++P +++    C + Y DAV+ ++D  + +   
Sbjct: 69  IIAISAASANASATSSY-IKQKLSNEDLEPAIEDTLEDCQKDYQDAVEQLDDSISAMLAD 128

Query: 126 DYNGVNIKASAAMTEANDCLDSFTQPPKDPSALSGNGKTVEDICSIILVIANLL 173
            +  V++  SAA++    C  +      + + LS   +    +C   L+I  +L
Sbjct: 129 AHTDVDVWLSAAISAIESCGSALGSRAGNDAELSQRNEVFLKLCKNALMINKML 174

[Close]

BLAST of CmoCh01G008870 vs. TAIR 10
Match: AT2G47340.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 48.9 bits (115), Expect = 4.8e-06
Identity = 28/99 (28.28%), Postives = 50/99 (50.51%), Query Frame = 0


Query: 71  ATQSYALAQSLASKAVDPKLKERYATCAELYDDAVDDIEDGKNYLGEGDYNGVNIKASAA 130
           AT  YA  + +  +     +KE  + C E YD  +  +ED K  + EGDY  ++   SAA
Sbjct: 179 ATLDYA-KRVVEDRKTPMPVKEAMSVCVENYDSLISGLEDAKMAMEEGDYGRLDSVLSAA 238

Query: 131 MTEANDCLDSFTQPPKDPSALSGNGKTVEDICSIILVIA 170
           +++ + C D+F   P   S  +     ++ +CS +L ++
Sbjct: 239 ISDVSTCSDNFVDIPDVDSPTASLDDLMKKLCSNVLAMS 276

The following BLAST results are available for this feature:

BLAST of CmoCh01G008870 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P83326	5.9e-33	43.50	Pectinesterase inhibitor OS=Actinidia deliciosa OX=3627 GN=PMEI PE=1 SV=2	[more]
Q9LNF2	6.8e-21	34.10	Pectinesterase inhibitor 1 OS=Arabidopsis thaliana OX=3702 GN=PMEI1 PE=1 SV=1	[more]
Q9LUV1	8.3e-19	36.55	Pectinesterase inhibitor 2 OS=Arabidopsis thaliana OX=3702 GN=PMEI2 PE=1 SV=1	[more]
Q9LUL8	1.2e-04	25.95	Putative pectinesterase/pectinesterase inhibitor 26 OS=Arabidopsis thaliana OX=3...	[more]

BLAST of CmoCh01G008870 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1GBD1	2.5e-82	100.00	pectinesterase inhibitor-like OS=Cucurbita moschata OX=3662 GN=LOC111452619 PE=4...	[more]
A0A6J1F4D5	3.9e-80	89.94	pectinesterase inhibitor-like OS=Cucurbita moschata OX=3662 GN=LOC111440166 PE=4...	[more]
A0A6J1EZI7	1.9e-79	89.39	pectinesterase inhibitor-like OS=Cucurbita moschata OX=3662 GN=LOC111440672 PE=4...	[more]
A0A6J1F469	4.3e-79	89.94	pectinesterase inhibitor-like OS=Cucurbita moschata OX=3662 GN=LOC111440164 PE=4...	[more]
A0A6J1F473	9.6e-79	88.83	pectinesterase inhibitor-like OS=Cucurbita moschata OX=3662 GN=LOC111440170 PE=4...	[more]

BLAST of CmoCh01G008870 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022949197.1	5.1e-82	100.00	pectinesterase inhibitor-like [Cucurbita moschata]	[more]
XP_022933240.1	8.1e-80	89.94	pectinesterase inhibitor-like [Cucurbita moschata]	[more]
XP_023521203.1	8.1e-80	90.50	pectinesterase inhibitor-like [Cucurbita pepo subsp. pepo]	[more]
XP_022933241.1	4.0e-79	89.39	pectinesterase inhibitor-like [Cucurbita moschata] >XP_022933352.1 pectinesteras...	[more]
XP_023522250.1	5.2e-79	89.94	pectinesterase inhibitor-like [Cucurbita pepo subsp. pepo]	[more]

BLAST of CmoCh01G008870 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G48020.1	4.8e-22	34.10	pectin methylesterase inhibitor 1	[more]
AT3G17220.1	5.9e-20	36.55	pectin methylesterase inhibitor 2	[more]
AT3G36659.1	8.0e-09	32.39	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT1G55770.1	1.7e-06	23.56	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT2G47340.1	4.8e-06	28.28	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006501	Pectinesterase inhibitor domain	SMART	SM00856	PMEI_2	coord: 26..168 e-value: 6.0E-35 score: 132.1
IPR006501	Pectinesterase inhibitor domain	PFAM	PF04043	PMEI	coord: 30..168 e-value: 7.8E-26 score: 91.2
IPR006501	Pectinesterase inhibitor domain	TIGRFAM	TIGR01614	TIGR01614	coord: 6..172 e-value: 2.3E-35 score: 119.5
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	GENE3D	1.20.140.40	Invertase/pectin methylesterase inhibitor family protein	coord: 26..174 e-value: 2.0E-40 score: 140.2
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	SUPERFAMILY	101148	Plant invertase/pectin methylesterase inhibitor	coord: 28..173
None	No IPR available	PANTHER	PTHR36710	PECTINESTERASE INHIBITOR-LIKE	coord: 3..172
None	No IPR available	PANTHER	PTHR36710:SF6	PECTINESTERASE INHIBITOR-LIKE	coord: 3..172
IPR034086	Pectinesterase inhibitor, plant	CDD	cd15797	PMEI	coord: 28..172 e-value: 1.34169E-47 score: 150.265

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmoCh01G008870.1	CmoCh01G008870.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0043086	negative regulation of catalytic activity
molecular_function	GO:0046910	pectinesterase inhibitor activity
molecular_function	GO:0004857	enzyme inhibitor activity