CmoCh01G008670 (gene) Cucurbita moschata (Rifu) v1

Overview


Name	CmoCh01G008670
Type	gene
Organism	Cucurbita moschata (Cucurbita moschata (Rifu) v1)
Description	Cystatin/monellin superfamily protein
Location	Cmo_Chr01: 4607410 .. 4608467 (+)
RNA-Seq Expression	CmoCh01G008670
Synteny	CmoCh01G008670

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCACCTCCCTACTCTGCTCGTACGTACAAACCAATTGATCCTTATGAGTCGGATGACGACTTGGCTGATGAACAGATGAGACTATACTATCAAGCCCTACGAGAGTCCGACGTATTATTCTTTTCCATTCTCCTCCTAATTTCCCTTTATTTTTAGTCCACGTTCAAATATTATTGCTTAATATCCATCGTCGTATGGCTACACAGGGTTTTGATGTATCCACTTTTTCCAACACCTCTCTTATGGGTCTCATTGCACCTATATGTGAGGAATGTCTATCTAGACCAAATCTTCGAGACTGTGCCGAGAAAGCTACTAGTGAGTACAACATGGAAAATATATGGACGTTTTACTTCACCTTCTCATTCTATATGTATTTCCTTTTATCAATAACCTTTCTAACCTCTTATTAAATCTTCTTTATGCCTAGGGTACAAACTTTGAGTTTGTGAGAGTTGTGAAGGCTAACCAACAAGCTGTGTCGGGTTTCTTGTATTACATCACTTTCGATGCCAAACAAATAGGAACAGAGCTTCCAACAACAACTTTCGAAGCTAAGGTCTTTGATAAGATTGATAATAGTAAAGAGATATGTTTATGTAGACCAAAACGCTTCGGTTGAGATACATCTTTTTAGTACGTGCATGTTCTTCTATCTAATAGACACTTTTTTCTCTATTTACATCGAGGGAGTATTTCCATATGGTTGGGGGAGTATTTCCACGCAGAACTATGTTTATGATTATATATATAGTTCTTATCATAATTAGTTTGGTTCAATATTTTTCTTTTTCCTTCTTCTTCCATGTGTGTTCATCTTGTTTGATGGGTAAGAAACAATAGCAAAGTTGAAGAACTTTATACCTCTAGACACATAAGAACTTTATACCTCTAGATGCATAGCTCGTCCTCTTTGTATTGCCTTTTGTTTGGATCCGAGTGATGCCTCTACAAATTTTTGCTTATTCCCAGATTATCTCTAGTTTCTCATAATGGATTCACTTTGGGGCGTGTTCATGTCCAACTTAATGACTGCCTTTAAGGCTCTAGCATGA

mRNA sequence

ATGGCACCTCCCTACTCTGCTCGTACGTACAAACCAATTGATCCTTATGAGTCGGATGACGACTTGGCTGATGAACAGATGAGACTATACTATCAAGCCCTACGAGAGTCCGACGGTACAAACTTTGAGTTTGTGAGAGTTGTGAAGGCTAACCAACAAGCTGTGTCGGGTTTCTTGTATTACATCACTTTCGATGCCAAACAAATAGGAACAGAGCTTCCAACAACAACTTTCGAAGCTAAGTTTCTCATAATGGATTCACTTTGGGGCGTGTTCATGTCCAACTTAATGACTGCCTTTAAGGCTCTAGCATGA

Coding sequence (CDS)

ATGGCACCTCCCTACTCTGCTCGTACGTACAAACCAATTGATCCTTATGAGTCGGATGACGACTTGGCTGATGAACAGATGAGACTATACTATCAAGCCCTACGAGAGTCCGACGGTACAAACTTTGAGTTTGTGAGAGTTGTGAAGGCTAACCAACAAGCTGTGTCGGGTTTCTTGTATTACATCACTTTCGATGCCAAACAAATAGGAACAGAGCTTCCAACAACAACTTTCGAAGCTAAGTTTCTCATAATGGATTCACTTTGGGGCGTGTTCATGTCCAACTTAATGACTGCCTTTAAGGCTCTAGCATGA

Protein sequence

MAPPYSARTYKPIDPYESDDDLADEQMRLYYQALRESDGTNFEFVRVVKANQQAVSGFLYYITFDAKQIGTELPTTTFEAKFLIMDSLWGVFMSNLMTAFKALA

Homology

[Close]

BLAST of CmoCh01G008670 vs. ExPASy TrEMBL
Match: A0A6J1G9T9 (uncharacterized protein LOC111452100 OS=Cucurbita moschata OX=3662 GN=LOC111452100 PE=4 SV=1)

HSP 1 Score: 64.7 bits (156), Expect = 2.6e-07
Identity = 30/46 (65.22%), Postives = 34/46 (73.91%), Query Frame = 0


Query: 38  DGTNFEFVRVVKANQQAVSGFLYYITFDAKQIGTELPTTTFEAKFL 84
           +GTN+EFV++ KA  Q   G LYYITFD KQIG E  TTTFEAK L
Sbjct: 85  NGTNYEFVKIAKATHQVAWGLLYYITFDVKQIGIEFLTTTFEAKVL 130

[Close]

BLAST of CmoCh01G008670 vs. ExPASy TrEMBL
Match: A0A6J1IJT3 (uncharacterized protein LOC111475320 OS=Cucurbita maxima OX=3661 GN=LOC111475320 PE=4 SV=1)

HSP 1 Score: 54.3 bits (129), Expect = 3.5e-04
Identity = 29/61 (47.54%), Postives = 39/61 (63.93%), Query Frame = 0


Query: 23  ADEQMRLYYQALRESDGTNFEFVRVVKANQQAVSGFLYYITFDAKQIGT--ELPTTTFEA 82
           A+E ++ Y       +GTNFE V +VKAN +   G +YYITF+ K IGT  E P+ TF+A
Sbjct: 80  ANEAIKHY----NSENGTNFEVVDIVKANHEGCCGSMYYITFNVKPIGTSAEFPSITFQA 136

[Close]

BLAST of CmoCh01G008670 vs. ExPASy TrEMBL
Match: A0A6J1C8H7 (uncharacterized protein LOC111008952 OS=Momordica charantia OX=3673 GN=LOC111008952 PE=4 SV=1)

HSP 1 Score: 53.5 bits (127), Expect = 6.0e-04
Identity = 28/69 (40.58%), Postives = 44/69 (63.77%), Query Frame = 0


Query: 17  ESDDDLADEQMRLYYQALRESDGTNFEFVRVVKANQQAVSGFLYYITFDAKQIGT--ELP 76
           E   + AD+   + Y    + +GT+FEFV++VKA  +AV G +YY+TF+ KQ+G+    P
Sbjct: 81  EELQECADKDKAVDY--FNQQNGTSFEFVKMVKATNKAVVGIMYYLTFEVKQMGSPPNSP 140

Query: 77  TTTFEAKFL 84
           T T +A+ L
Sbjct: 141 TKTLQARVL 147

[Close]

BLAST of CmoCh01G008670 vs. ExPASy TrEMBL
Match: A0A6J1FN74 (uncharacterized protein LOC111447330 OS=Cucurbita moschata OX=3662 GN=LOC111447330 PE=4 SV=1)

HSP 1 Score: 53.5 bits (127), Expect = 6.0e-04
Identity = 29/61 (47.54%), Postives = 38/61 (62.30%), Query Frame = 0


Query: 23  ADEQMRLYYQALRESDGTNFEFVRVVKANQQAVSGFLYYITFDAKQIGT--ELPTTTFEA 82
           A+E ++ Y       +GTNFE V +VKAN     G +YYITF+ K IGT  E P+ TF+A
Sbjct: 80  ANEAIKHY----NNENGTNFEVVDIVKANHSGCCGSMYYITFNVKPIGTPLEFPSITFQA 136

[Close]

BLAST of CmoCh01G008670 vs. ExPASy TrEMBL
Match: A0A6J1FVU0 (uncharacterized protein LOC111447329 OS=Cucurbita moschata OX=3662 GN=LOC111447329 PE=4 SV=1)

HSP 1 Score: 53.1 bits (126), Expect = 7.9e-04
Identity = 31/74 (41.89%), Postives = 43/74 (58.11%), Query Frame = 0


Query: 12  PIDPYESDDDLADEQM--RLYYQALRESDGTNFEFVRVVKANQQAVSGFLYYITFDAKQI 71
           PID +E+   L + ++      +   + +GTNFE V +VKAN     G +YYITF+ K I
Sbjct: 131 PIDMHENSWMLKEVRISANKAIKHYNKENGTNFEVVEIVKANHSGCCGSMYYITFNVKPI 190

Query: 72  GT--ELPTTTFEAK 82
           GT  E P  TF+AK
Sbjct: 191 GTSAEFPPITFQAK 204

[Close]

BLAST of CmoCh01G008670 vs. NCBI nr
Match: XP_023525925.1 (uncharacterized protein LOC111789396 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 82.8 bits (203), Expect = 1.9e-12
Identity = 38/46 (82.61%), Postives = 41/46 (89.13%), Query Frame = 0


Query: 38  DGTNFEFVRVVKANQQAVSGFLYYITFDAKQIGTELPTTTFEAKFL 84
           +GTNFEFV++VKANQQ VSGF YYITFD KQIGTE PTTTFEAK L
Sbjct: 120 NGTNFEFVKIVKANQQVVSGFFYYITFDVKQIGTEFPTTTFEAKVL 165

[Close]

BLAST of CmoCh01G008670 vs. NCBI nr
Match: KAG6607422.1 (hypothetical protein SDJN03_00764, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 72.0 bits (175), Expect = 3.4e-09
Identity = 52/135 (38.52%), Postives = 64/135 (47.41%), Query Frame = 0


Query: 1   MAPPYSARTYKPIDPYES-DDDLADEQMRLYYQALRESD--------------------- 60
           M PP   +T +P +PY+S DDDL  E++  Y +A   SD                     
Sbjct: 1   MTPP--VQTNQPNNPYDSDDDDLTPEELSRYNEAALASDGFDVPTLRDSPEVGLIVPVSE 60

Query: 61  -----------------------GTNFEFVRVVKANQQAVSGFLYYITFDAKQIGTELPT 89
                                  GTN+EFV++VKAN +   G LYYITFD KQIGTE  T
Sbjct: 61  KLLSKPYLRECAEKAISQYNIENGTNYEFVKIVKANHRVAWGLLYYITFDVKQIGTEFLT 120

[Close]

BLAST of CmoCh01G008670 vs. NCBI nr
Match: KAG6607423.1 (hypothetical protein SDJN03_00765, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 68.9 bits (167), Expect = 2.9e-08
Identity = 37/61 (60.66%), Postives = 43/61 (70.49%), Query Frame = 0


Query: 38  DGTNFEFVRVVKANQQAVSGFLYYITFDAKQIGTELPTTTFEAKFL--IMDSLWGVFMSN 97
           +GTN+EFV++VKA  QA  G LYYITFD KQIG E  TTTFEAK L  I DS   +F  +
Sbjct: 85  NGTNYEFVKIVKATHQASWGLLYYITFDVKQIGIEFLTTTFEAKVLHAIDDSAHSIFRLS 144

[Close]

BLAST of CmoCh01G008670 vs. NCBI nr
Match: XP_023525978.1 (uncharacterized protein LOC111789436 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 67.0 bits (162), Expect = 1.1e-07
Identity = 32/46 (69.57%), Postives = 36/46 (78.26%), Query Frame = 0


Query: 38  DGTNFEFVRVVKANQQAVSGFLYYITFDAKQIGTELPTTTFEAKFL 84
           +GTN+EFV++VKA  QA  G LYYITFD KQIG E  TTTFEAK L
Sbjct: 85  NGTNYEFVKIVKATHQASWGLLYYITFDVKQIGIEFLTTTFEAKVL 130

[Close]

BLAST of CmoCh01G008670 vs. NCBI nr
Match: KAG6607430.1 (hypothetical protein SDJN03_00772, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 67.0 bits (162), Expect = 1.1e-07
Identity = 32/46 (69.57%), Postives = 36/46 (78.26%), Query Frame = 0


Query: 38  DGTNFEFVRVVKANQQAVSGFLYYITFDAKQIGTELPTTTFEAKFL 84
           +GTN+EFV++VKA  QA  G LYYITFD KQIG E  TTTFEAK L
Sbjct: 85  NGTNYEFVKIVKATHQASWGLLYYITFDVKQIGIEFLTTTFEAKVL 130

[Close]

BLAST of CmoCh01G008670 vs. TAIR 10
Match: AT1G50690.1 (Cystatin/monellin superfamily protein )

HSP 1 Score: 49.3 bits (116), Expect = 2.2e-06
Identity = 24/64 (37.50%), Postives = 36/64 (56.25%), Query Frame = 0


Query: 17  ESDDDLADEQMRLYYQALRESDGTNFEFVRVVKANQQAVSGFLYYITFDAKQIGTELPTT 76
           E+D DL     ++  Q   +    N E VR VKAN++  +GF++YITF+AK   +     
Sbjct: 88  ETDGDLVHRLSKIALQKYNDDKLENLELVRAVKANRKYGAGFIFYITFEAKDANSHTDPI 147

Query: 77  TFEA 81
           TF+A
Sbjct: 148 TFQA 151

[Close]

BLAST of CmoCh01G008670 vs. TAIR 10
Match: AT2G37435.1 (Cystatin/monellin superfamily protein )

HSP 1 Score: 46.2 bits (108), Expect = 1.9e-05
Identity = 25/80 (31.25%), Postives = 41/80 (51.25%), Query Frame = 0


Query: 10  YKPIDPYESDDDLADEQMRLYYQAL--------RESDGTNFEFVRVVKANQQAVSGFLYY 69
           Y+P+D  +++  L  E  R +   L         E   T +EFVR +KAN    +G +Y+
Sbjct: 75  YRPVDLDDNELALEPETTREFMDRLCRKSLEHFNEIHSTKYEFVRFIKANHHVSAGMMYF 134

Query: 70  ITFDAKQIGTELPTTTFEAK 82
           ITF+ K +  +  +  F+AK
Sbjct: 135 ITFEGKLLSNDDDSKQFQAK 154

[Close]

BLAST of CmoCh01G008670 vs. TAIR 10
Match: AT4G15715.1 (Proteinase inhibitor I25, cystatin, conserved region )

HSP 1 Score: 42.0 bits (97), Expect = 3.5e-04
Identity = 20/39 (51.28%), Postives = 24/39 (61.54%), Query Frame = 0


Query: 36 ESDGTNFEFVRVVKANQQAVSGFLYYITFDAKQIGTELP 75
          E  GT  EFV+ V AN   V GFL+YITF AK + +  P
Sbjct: 52 EETGTRLEFVKYVSANVLGVQGFLFYITFWAKDLSSPNP 90

The following BLAST results are available for this feature:

BLAST of CmoCh01G008670 vs.


Match Name	E-value	Identity	Description

BLAST of CmoCh01G008670 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1G9T9	2.6e-07	65.22	uncharacterized protein LOC111452100 OS=Cucurbita moschata OX=3662 GN=LOC1114521...	[more]
A0A6J1IJT3	3.5e-04	47.54	uncharacterized protein LOC111475320 OS=Cucurbita maxima OX=3661 GN=LOC111475320...	[more]
A0A6J1C8H7	6.0e-04	40.58	uncharacterized protein LOC111008952 OS=Momordica charantia OX=3673 GN=LOC111008...	[more]
A0A6J1FN74	6.0e-04	47.54	uncharacterized protein LOC111447330 OS=Cucurbita moschata OX=3662 GN=LOC1114473...	[more]
A0A6J1FVU0	7.9e-04	41.89	uncharacterized protein LOC111447329 OS=Cucurbita moschata OX=3662 GN=LOC1114473...	[more]

BLAST of CmoCh01G008670 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023525925.1	1.9e-12	82.61	uncharacterized protein LOC111789396 [Cucurbita pepo subsp. pepo]	[more]
KAG6607422.1	3.4e-09	38.52	hypothetical protein SDJN03_00764, partial [Cucurbita argyrosperma subsp. sorori...	[more]
KAG6607423.1	2.9e-08	60.66	hypothetical protein SDJN03_00765, partial [Cucurbita argyrosperma subsp. sorori...	[more]
XP_023525978.1	1.1e-07	69.57	uncharacterized protein LOC111789436 [Cucurbita pepo subsp. pepo]	[more]
KAG6607430.1	1.1e-07	69.57	hypothetical protein SDJN03_00772, partial [Cucurbita argyrosperma subsp. sorori...	[more]

BLAST of CmoCh01G008670 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G50690.1	2.2e-06	37.50	Cystatin/monellin superfamily protein	[more]
AT2G37435.1	1.9e-05	31.25	Cystatin/monellin superfamily protein	[more]
AT4G15715.1	3.5e-04	51.28	Proteinase inhibitor I25, cystatin, conserved region	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006525	Cystatin-related, plant	TIGRFAM	TIGR01638	TIGR01638	coord: 13..78 e-value: 5.3E-9 score: 34.1
IPR000010	Cystatin domain	PFAM	PF00031	Cystatin	coord: 36..68 e-value: 4.9E-6 score: 26.7
IPR000010	Cystatin domain	CDD	cd00042	CY	coord: 36..68 e-value: 3.48729E-7 score: 42.8464
None	No IPR available	GENE3D	3.10.450.10		coord: 9..96 e-value: 8.8E-10 score: 40.7
None	No IPR available	SUPERFAMILY	54403	Cystatin/monellin	coord: 17..68

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmoCh01G008670.1	CmoCh01G008670.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0010466	negative regulation of peptidase activity
molecular_function	GO:0004869	cysteine-type endopeptidase inhibitor activity