CmoCh01G006910 (gene) Cucurbita moschata (Rifu) v1

Overview


Name	CmoCh01G006910
Type	gene
Organism	Cucurbita moschata (Cucurbita moschata (Rifu) v1)
Description	3-oxo-Delta(4,5)-steroid 5-beta-reductase-like
Location	Cmo_Chr01: 3534224 .. 3535499 (-)
RNA-Seq Expression	CmoCh01G006910
Synteny	CmoCh01G006910

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCGGCCGAAACAATCAATGCAAAATTAGACGGCAATGGCGAACTTGTCGCCGTCATTTTCGGAGTCACCGGTTTGGTCGGAAAAGAGTTAGCAAAAGCCCTTCTCTCAACCGGCGGCTGGAAAGTTTACGGCGTCGCACGGCGGCCGGATAATGTCAGCCCCATCACCGATACTAATTTTCAATTCATTTCCTGCGATTTGCTCGACCGTTCTTCCGCTCACCGAAACCTCTCGCCGGTTCGCCACGTCACACATGTTTTCTGGGTCACTTGGGCTGCCCAGCTACGGTTCGATACCTCAGATTGCTCGGACCAGAACCGGACCATGTTGGCTAACGTCCTCGACGTCATTCTCCCGGCGGCGCCGGCTCTCCGCCACGTCTCTCTACAGACCGGGATTAAGCACTACGTTTCGTTGACGGGAGCCGCCGTCGGAGGCGGCGGCGGTCGGATGGCGGTTTATTGTGAGGATAGTCCGAGGGCGGAATCTGGGAACAATTTTTATTATGCGTTGGAGGATCTCCTCCGTGAGCGGCTGTCTGGAGGCGGGCGGAGGGTGGCGTGGAGTGTTCTTCGACCGGGTTTGATTTTGGGCTGTTCGAACCGGACTTTTTTTAATTTTATGGGGAGTTTGTGTGTTTATGGAGCTATTTGTAAGAAGCTGAAGAAGCCGTTTGTGTTCGGCGGCTCGGCGGCGTGCTGGGAAGGGTTTTGTATCAATGGCTCCGACGCTCGGCTTGTGGCGGAGCAGCACGTTTGGGCCGCCACCAATGAAATAAACAGTACGCCACTCTTTCACTATTAATTTATTTTTAATAATTAATTTCACTTTTCCATAAAAATATTTATATAAAATATTTTATGTGTAAATTCCAACGACAGCCACCGCAGACGGGGAAGCATTCAACGTGTGCAACGGGTGGAGCTTCACGTGGAAGGAGATATGGGGAGCGGTGGCGGAGAAGTTTGGAGTGGCGGCGGCGGCGGAGGACGAGGAGGGGATGTTTTCTCCGGAGTTCCATTACACGGCAGCGATGACGGAGAAAGGGAGGTTGTGGGCGGAGATAGTGGAGGAGGAAGGGCTGATTCCGACGGAAATGGAGGAGCTGGCCAATTGGGGGTTCTTAGACAGCTTGTTTAGGCTGCCGGGAAAAATGGTGGCTAGTCGGAACAAATCCGACCGGCTTGGATTCAGTGTAAAGTACAAGATGTTGGACTCCATCTTGTATTGGATCGATGTCATGAGATACGATAAATTAATTCCTTTTCCTTAA

mRNA sequence

ATGGCGGCCGAAACAATCAATGCAAAATTAGACGGCAATGGCGAACTTGTCGCCGTCATTTTCGGAGTCACCGGTTTGGTCGGAAAAGAGTTAGCAAAAGCCCTTCTCTCAACCGGCGGCTGGAAAGTTTACGGCGTCGCACGGCGGCCGGATAATGTCAGCCCCATCACCGATACTAATTTTCAATTCATTTCCTGCGATTTGCTCGACCGTTCTTCCGCTCACCGAAACCTCTCGCCGGTTCGCCACGTCACACATGTTTTCTGGGTCACTTGGGCTGCCCAGCTACGGTTCGATACCTCAGATTGCTCGGACCAGAACCGGACCATGTTGGCTAACGTCCTCGACGTCATTCTCCCGGCGGCGCCGGCTCTCCGCCACGTCTCTCTACAGACCGGGATTAAGCACTACGTTTCGTTGACGGGAGCCGCCGTCGGAGGCGGCGGCGGTCGGATGGCGGTTTATTGTGAGGATAGTCCGAGGGCGGAATCTGGGAACAATTTTTATTATGCGTTGGAGGATCTCCTCCGTGAGCGGCTGTCTGGAGGCGGGCGGAGGGTGGCGTGGAGTGTTCTTCGACCGGGTTTGATTTTGGGCTGTTCGAACCGGACTTTTTTTAATTTTATGGGGAGTTTGTGTGTTTATGGAGCTATTTGTAAGAAGCTGAAGAAGCCGTTTGTGTTCGGCGGCTCGGCGGCGTGCTGGGAAGGGTTTTGTATCAATGGCTCCGACGCTCGGCTTGTGGCGGAGCAGCACGTTTGGGCCGCCACCAATGAAATAAACACCACCGCAGACGGGGAAGCATTCAACGTGTGCAACGGGTGGAGCTTCACGTGGAAGGAGATATGGGGAGCGGTGGCGGAGAAGTTTGGAGTGGCGGCGGCGGCGGAGGACGAGGAGGGGATGTTTTCTCCGGAGTTCCATTACACGGCAGCGATGACGGAGAAAGGGAGGTTGTGGGCGGAGATAGTGGAGGAGGAAGGGCTGATTCCGACGGAAATGGAGGAGCTGGCCAATTGGGGGTTCTTAGACAGCTTGTTTAGGCTGCCGGGAAAAATGGTGGCTAGTCGGAACAAATCCGACCGGCTTGGATTCAGTGTAAAGTACAAGATGTTGGACTCCATCTTGTATTGGATCGATGTCATGAGATACGATAAATTAATTCCTTTTCCTTAA

Coding sequence (CDS)

ATGGCGGCCGAAACAATCAATGCAAAATTAGACGGCAATGGCGAACTTGTCGCCGTCATTTTCGGAGTCACCGGTTTGGTCGGAAAAGAGTTAGCAAAAGCCCTTCTCTCAACCGGCGGCTGGAAAGTTTACGGCGTCGCACGGCGGCCGGATAATGTCAGCCCCATCACCGATACTAATTTTCAATTCATTTCCTGCGATTTGCTCGACCGTTCTTCCGCTCACCGAAACCTCTCGCCGGTTCGCCACGTCACACATGTTTTCTGGGTCACTTGGGCTGCCCAGCTACGGTTCGATACCTCAGATTGCTCGGACCAGAACCGGACCATGTTGGCTAACGTCCTCGACGTCATTCTCCCGGCGGCGCCGGCTCTCCGCCACGTCTCTCTACAGACCGGGATTAAGCACTACGTTTCGTTGACGGGAGCCGCCGTCGGAGGCGGCGGCGGTCGGATGGCGGTTTATTGTGAGGATAGTCCGAGGGCGGAATCTGGGAACAATTTTTATTATGCGTTGGAGGATCTCCTCCGTGAGCGGCTGTCTGGAGGCGGGCGGAGGGTGGCGTGGAGTGTTCTTCGACCGGGTTTGATTTTGGGCTGTTCGAACCGGACTTTTTTTAATTTTATGGGGAGTTTGTGTGTTTATGGAGCTATTTGTAAGAAGCTGAAGAAGCCGTTTGTGTTCGGCGGCTCGGCGGCGTGCTGGGAAGGGTTTTGTATCAATGGCTCCGACGCTCGGCTTGTGGCGGAGCAGCACGTTTGGGCCGCCACCAATGAAATAAACACCACCGCAGACGGGGAAGCATTCAACGTGTGCAACGGGTGGAGCTTCACGTGGAAGGAGATATGGGGAGCGGTGGCGGAGAAGTTTGGAGTGGCGGCGGCGGCGGAGGACGAGGAGGGGATGTTTTCTCCGGAGTTCCATTACACGGCAGCGATGACGGAGAAAGGGAGGTTGTGGGCGGAGATAGTGGAGGAGGAAGGGCTGATTCCGACGGAAATGGAGGAGCTGGCCAATTGGGGGTTCTTAGACAGCTTGTTTAGGCTGCCGGGAAAAATGGTGGCTAGTCGGAACAAATCCGACCGGCTTGGATTCAGTGTAAAGTACAAGATGTTGGACTCCATCTTGTATTGGATCGATGTCATGAGATACGATAAATTAATTCCTTTTCCTTAA

Protein sequence

MAAETINAKLDGNGELVAVIFGVTGLVGKELAKALLSTGGWKVYGVARRPDNVSPITDTNFQFISCDLLDRSSAHRNLSPVRHVTHVFWVTWAAQLRFDTSDCSDQNRTMLANVLDVILPAAPALRHVSLQTGIKHYVSLTGAAVGGGGGRMAVYCEDSPRAESGNNFYYALEDLLRERLSGGGRRVAWSVLRPGLILGCSNRTFFNFMGSLCVYGAICKKLKKPFVFGGSAACWEGFCINGSDARLVAEQHVWAATNEINTTADGEAFNVCNGWSFTWKEIWGAVAEKFGVAAAAEDEEGMFSPEFHYTAAMTEKGRLWAEIVEEEGLIPTEMEELANWGFLDSLFRLPGKMVASRNKSDRLGFSVKYKMLDSILYWIDVMRYDKLIPFP

Homology

[Close]

BLAST of CmoCh01G006910 vs. ExPASy Swiss-Prot
Match: A0A1C9CX66 ((S)-8-oxocitronellyl enol synthase CYC2 OS=Camptotheca acuminata OX=16922 GN=CYC2 PE=1 SV=1)

HSP 1 Score: 255.4 bits (651), Expect = 1.1e-66
Identity = 159/378 (42.06%), Postives = 209/378 (55.29%), Query Frame = 0


Query: 17  VAVIFGVTGLVGKELAKAL----LSTGGWKVYGVARRPDNVSPITDTNFQFISCDLLDRS 76
           VA+I GVTG+VG  LA+ L       G WKVYGVARRP   +   D   ++I CD+ D S
Sbjct: 28  VALIIGVTGIVGNSLAEILPISDTPGGPWKVYGVARRP-RPAWNADHPVEYIQCDISDAS 87

Query: 77  SAHRNLSPVRHVTHVFWVTWAAQLRFDTSDCSDQNRTMLANVLDVILPAAPALRHVSLQT 136
             H  LSP+  VTH+FWVTWA   R   S+C + N TML NVL+ ++P A  L H+ LQT
Sbjct: 88  DTHTKLSPLTDVTHIFWVTWAN--RPTESECCELNGTMLRNVLNALIPKAANLHHICLQT 147

Query: 137 GIKHYVSLTGAAVGGGGGRMAVYCEDSPRAESGNNFYYALEDLLRERLSGGGRRVAWSVL 196
           G KHY+     A G        + ED PR  +  NFYY LED+L E  S     + WSV 
Sbjct: 148 GHKHYIG-PFEAFGKIKPHEPPFTEDMPRL-NAPNFYYTLEDMLVE-ASEKKAGLNWSVH 207

Query: 197 RPGLILGCSNRTFFNFMGSLCVYGAICKKLKKPFVFGGSAACWEGFCINGSDARLVAEQH 256
           RP +I G S  +  N +G+LCVY AICK    P  F G+ A W  + +  SDA L+AE  
Sbjct: 208 RPAVIFGFSPFSMMNIIGTLCVYAAICKHENTPLKFPGTKAAWNCYSV-ASDADLIAEHQ 267

Query: 257 VWAATNEINTTADGEAFNVCNGWSFTWKEIWGAVAEKFGVAAAAEDEEGMFSPEFHYTAA 316
           +WAA   ++  A  EAFN  NG  F WK +W  +AE+FGV  A  DE             
Sbjct: 268 IWAA---VDPYAKNEAFNCSNGDLFKWKHLWKVLAEQFGVEYAEFDES---EKPTSLVER 327

Query: 317 MTEKGRLWAEIVEEEGLIPTEMEELANWGFLDSLFRLPGK-MVASRNKSDRLGFSVKYKM 376
           M +KG +W EIV E GL  T++E +A W F D +  L G+ ++ S NKS   G+      
Sbjct: 328 MKDKGPVWEEIVRENGLHTTKLEGVATWWFADVI--LGGECLLDSMNKSKEHGYLGFRNT 387

Query: 377 LDSILYWIDVMRYDKLIP 390
            +S++  ID M+  K++P
Sbjct: 388 KNSLISVIDKMKAHKIVP 390

[Close]

BLAST of CmoCh01G006910 vs. ExPASy Swiss-Prot
Match: Q9STX2 (3-oxo-Delta(4,5)-steroid 5-beta-reductase OS=Arabidopsis thaliana OX=3702 GN=VEP1 PE=1 SV=1)

HSP 1 Score: 250.4 bits (638), Expect = 3.4e-65
Identity = 156/380 (41.05%), Postives = 203/380 (53.42%), Query Frame = 0


Query: 15  ELVAVIFGVTGLVGKELAKAL----LSTGGWKVYGVARRPDNVSPITDTNFQFISCDLLD 74
           E VA+I GVTG+VG  LA+ L       G WKVYGVARRP   +   D    +I CD+ D
Sbjct: 26  ESVALIIGVTGIVGNSLAEILPLSDTPGGPWKVYGVARRP-RPTWNADHPIDYIQCDVSD 85

Query: 75  RSSAHRNLSPVRHVTHVFWVTWAAQLRFDTSDCSDQNRTMLANVLDVILPAAPALRHVSL 134
                  LSP+  VTHVF+VTW    R   S+  + N +ML NVL  I+P AP LRHV L
Sbjct: 86  AEDTRSKLSPLTDVTHVFYVTWTN--RESESENCEANGSMLRNVLQAIIPYAPNLRHVCL 145

Query: 135 QTGIKHYVSLTGAAVGGGGGRM-AVYCEDSPRAESGNNFYYALEDLLRERLSGGGRRVAW 194
           QTG KHY+   G      G R    + ED PR +   NFYY  ED+L E +      V W
Sbjct: 146 QTGTKHYL---GPFTNVDGPRHDPPFTEDMPRLQI-QNFYYTQEDILFEEIK-KIETVTW 205

Query: 195 SVLRPGLILGCSNRTFFNFMGSLCVYGAICKKLKKPFVFGGSAACWEGFCINGSDARLVA 254
           S+ RP +I G S  +  N +G+LCVY AICK    P +F GS   WEGF +  SDA L+A
Sbjct: 206 SIHRPNMIFGFSPYSLMNIVGTLCVYAAICKHEGSPLLFPGSKKAWEGF-MTASDADLIA 265

Query: 255 EQHVWAATNEINTTADGEAFNVCNGWSFTWKEIWGAVAEKFGVAAAAEDEEGMFSPEFHY 314
           EQ +WAA   ++  A  EAFN  N   F WK +W  +AE+FG+     +E          
Sbjct: 266 EQQIWAA---VDPYAKNEAFNCNNADIFKWKHLWKILAEQFGIEEYGFEE----GKNLGL 325

Query: 315 TAAMTEKGRLWAEIVEEEGLIPTEMEELANWGFLDSLFRLPGKMVASRNKSDRLGFSVKY 374
              M  K R+W E+V+E  L   ++EE+  W F D +  + G M+ S NKS   GF    
Sbjct: 326 VEMMKGKERVWEEMVKENQLQEKKLEEVGVWWFADVILGVEG-MIDSMNKSKEYGFLGFR 385

Query: 375 KMLDSILYWIDVMRYDKLIP 390
              +S + WID  +  K++P
Sbjct: 386 NSNNSFISWIDKYKAFKIVP 388

[Close]

BLAST of CmoCh01G006910 vs. ExPASy Swiss-Prot
Match: Q6PQJ9 (3-oxo-Delta(4,5)-steroid 5-beta-reductase OS=Digitalis lanata OX=49450 PE=1 SV=1)

HSP 1 Score: 240.7 bits (613), Expect = 2.7e-62
Identity = 151/377 (40.05%), Postives = 203/377 (53.85%), Query Frame = 0


Query: 17  VAVIFGVTGLVGKELAKAL----LSTGGWKVYGVARRPDNVSPITDTNFQFISCDLLDRS 76
           VA+I GVTG++G  LA+ L       G WKVYGVARR    +   D    ++ CD+ D  
Sbjct: 28  VALIVGVTGIIGNSLAEILPLADTPGGPWKVYGVARR-TRPAWHEDNPINYVQCDISDPD 87

Query: 77  SAHRNLSPVRHVTHVFWVTWAAQLRFDTSDCSDQNRTMLANVLDVILPAAPALRHVSLQT 136
            +   LSP+  VTHVF+VTWA   R    +  + N  M  NVLD ++P  P L+H+SLQT
Sbjct: 88  DSQAKLSPLTDVTHVFYVTWAN--RSTEQENCEANSKMFRNVLDAVIPNCPNLKHISLQT 147

Query: 137 GIKHYVSLTGAAVGGGGGRMAVYCEDSPRAESGNNFYYALEDLLRERLSGGGRRVAWSVL 196
           G KHY+     + G        Y ED PR +   NFYY LED++ E +      + WSV 
Sbjct: 148 GRKHYMG-PFESYGKIESHDPPYTEDLPRLKY-MNFYYDLEDIMLEEVE-KKEGLTWSVH 207

Query: 197 RPGLILGCSNRTFFNFMGSLCVYGAICKKLKKPFVFGGSAACWEGFCINGSDARLVAEQH 256
           RPG I G S  +  N +G+LCVY AICK   K   F G  A W+G+  + SDA L+AE H
Sbjct: 208 RPGNIFGFSPYSMMNLVGTLCVYAAICKHEGKVLRFTGCKAAWDGYS-DCSDADLIAEHH 267

Query: 257 VWAATNEINTTADGEAFNVCNGWSFTWKEIWGAVAEKFGVAAAAEDEEGMFSPEFHYTAA 316
           +WAA   ++  A  EAFNV NG  F WK  W  +AE+FGV    E EEG+   +      
Sbjct: 268 IWAA---VDPYAKNEAFNVSNGDVFKWKHFWKVLAEQFGV-GCGEYEEGV---DLKLQDL 327

Query: 317 MTEKGRLWAEIVEEEGLIPTEMEELANWGFLDSLFRLPGKMVASRNKSDRLGFSVKYKML 376
           M  K  +W EIV E GL PT+++++  W F D +       + S NKS   GF       
Sbjct: 328 MKGKEPVWEEIVRENGLTPTKLKDVGIWWFGDVILG-NECFLDSMNKSKEHGFLGFRNSK 387

Query: 377 DSILYWIDVMRYDKLIP 390
           ++ + WID  +  K++P
Sbjct: 388 NAFISWIDKAKAYKIVP 389

[Close]

BLAST of CmoCh01G006910 vs. ExPASy Swiss-Prot
Match: A0A221J5X3 ((S)-8-oxocitronellyl enol synthase ISY1 OS=Nepeta racemosa OX=54731 GN=ISY1 PE=1 SV=1)

HSP 1 Score: 239.2 bits (609), Expect = 7.9e-62
Identity = 156/382 (40.84%), Postives = 204/382 (53.40%), Query Frame = 0


Query: 13  NGELVAVIFGVTGLVGKELAKAL----LSTGGWKVYGVARRPDNVSPITDTNFQFISCDL 72
           N E VA+I GVTG+VG  LA+ L       G WKVYGVARRP   S   D    +ISCD+
Sbjct: 24  NYESVALIVGVTGIVGNSLAEILPLSDTPCGPWKVYGVARRP-RPSWNEDHPITYISCDV 83

Query: 73  LDRSSAHRNLSPVRHVTHVFWVTWAAQLRFDTSDCSDQNRTMLANVLDVILPAAPALRHV 132
           LD       LSP+  VTH+F+ TW    R    +  + N  ML NVL+ ++P  P L+H+
Sbjct: 84  LDSVDVEAKLSPLTDVTHIFYATWTK--RSTEKENCEANGKMLKNVLNAMIPNCPNLKHI 143

Query: 133 SLQTGIKHYVSLTGAAVGGGGGRM-AVYCEDSPRAESGNNFYYALEDLLRERLSGGGRRV 192
            LQTG KHY+   GA       R      ED PR +S  NFYY  ED+L E +      +
Sbjct: 144 CLQTGRKHYL---GAFEDWKIKRHDPPLTEDLPRLDS-QNFYYTQEDILFEEVQ-KKESL 203

Query: 193 AWSVLRPGLILGCSNRTFFNFMGSLCVYGAICKKLKKPFVFGGSAACWEGFCINGSDARL 252
            WSV RPG I G S  +  N +G+LCVY AICK       F G    W+G+  + SDA L
Sbjct: 204 TWSVHRPGTIFGFSPYSMMNLVGTLCVYAAICKHEGAVLRFPGCKGAWDGYS-DCSDADL 263

Query: 253 VAEQHVWAATNEINTTADGEAFNVCNGWSFTWKEIWGAVAEKFGVAAAAEDEEGMFSPEF 312
           +AE  +WAA   ++  A  EAFNV NG  F WK  W  +AE+FGV    E EEG    E 
Sbjct: 264 IAEHQIWAA---VDPYAKNEAFNVSNGDVFKWKHFWKVLAEQFGV-ECGEYEEG---KEV 323

Query: 313 HYTAAMTEKGRLWAEIVEEEGLIPTEMEELANWGFLDSLFRLPGKMVASRNKSDRLGFSV 372
                M +KG +W +IV E GL  T++E++ NW F D +       + + NKS   GF  
Sbjct: 324 KLQDLMKDKGPVWDKIVRENGLSTTKLEDVGNWWFSDIVLG-NECWLDTMNKSKEHGFLG 383

Query: 373 KYKMLDSILYWIDVMRYDKLIP 390
                +S + WID ++  K++P
Sbjct: 384 FRNSKNSFISWIDKVKAFKIVP 388

[Close]

BLAST of CmoCh01G006910 vs. ExPASy Swiss-Prot
Match: A0A221J5X1 ((S)-8-oxocitronellyl enol synthase ISY2 OS=Nepeta cataria OX=39347 GN=ISY2 PE=1 SV=1)

HSP 1 Score: 238.8 bits (608), Expect = 1.0e-61
Identity = 148/381 (38.85%), Postives = 206/381 (54.07%), Query Frame = 0


Query: 15  ELVAVIFGVTGLVGKELAKAL----LSTGGWKVYGVARRPDNVSPITDTNFQFISCDLLD 74
           + VA+I GVTGLVG  LA+ L       G WKVYGVARRP   S   D    +ISCD+ +
Sbjct: 27  QCVALIVGVTGLVGNSLAEMLPLSDTPGGPWKVYGVARRP-RPSWNEDHPINYISCDVSN 86

Query: 75  RSSAHRNLSPVRHVTHVFWVTWAAQLRFDTSDCSDQNRTMLANVLDVILPAAPALRHVSL 134
            +     L P+  VTH+F+ TW +  R    +  + N  ML NVLD ++P  P L+H+ L
Sbjct: 87  TAEVEAKLPPLSDVTHIFYATWTS--RSSEEENCEANGKMLKNVLDTMIPNCPNLKHICL 146

Query: 135 QTGIKHYV-SLTGAAVGGGGGRMAVYCEDSPRAESGNNFYYALEDLLRERLSGGGRRVAW 194
           QTG  HYV S+    +   G       ED PR  + NNFYY  ED+L E +      + W
Sbjct: 147 QTGRFHYVASVVDWKI--NGSHDTPLTEDLPRLNT-NNFYYTQEDILLEEVK-RKEGLTW 206

Query: 195 SVLRPGLILGCSNRTFFNFMGSLCVYGAICKKLKKPFVFGGSAACWEGFCINGSDARLVA 254
           SV RPG I G S  +  N +G+LCVY AICK+      F G    W+G+  + +DA L+A
Sbjct: 207 SVHRPGTIFGFSPYSMMNLVGTLCVYAAICKQEGAVLRFPGCKGAWDGYS-DCADADLIA 266

Query: 255 EQHVWAATNEINTTADGEAFNVCNGWSFTWKEIWGAVAEKFGVAAAA-EDEEGMFSPEFH 314
           E ++WAA   ++  A  ++FNV NG  F WK +W  +AE+FGV     E EEG    +  
Sbjct: 267 EHYIWAA---LDPHAKNQSFNVSNGDVFKWKHLWKVLAEQFGVECGGYEYEEGQ---QVR 326

Query: 315 YTAAMTEKGRLWAEIVEEEGLIPTEMEELANWGFLDSLFRLPGKMVASRNKSDRLGFSVK 374
               M +KG +W +IV E GL  T++E++  W F D++     ++  S NKS   GF   
Sbjct: 327 LQDVMKDKGPVWDKIVRENGLSNTKLEDVGKWWFSDTILWNECRL-DSMNKSKEHGFLGF 386

Query: 375 YKMLDSILYWIDVMRYDKLIP 390
               +  LYWI  ++  K++P
Sbjct: 387 RNSKNCFLYWIHKLKAYKIVP 392

[Close]

BLAST of CmoCh01G006910 vs. ExPASy TrEMBL
Match: A0A6J1GA93 (3-oxo-Delta(4,5)-steroid 5-beta-reductase-like OS=Cucurbita moschata OX=3662 GN=LOC111452342 PE=3 SV=1)

HSP 1 Score: 808.1 bits (2086), Expect = 1.6e-230
Identity = 391/391 (100.00%), Postives = 391/391 (100.00%), Query Frame = 0


Query: 1   MAAETINAKLDGNGELVAVIFGVTGLVGKELAKALLSTGGWKVYGVARRPDNVSPITDTN 60
           MAAETINAKLDGNGELVAVIFGVTGLVGKELAKALLSTGGWKVYGVARRPDNVSPITDTN
Sbjct: 1   MAAETINAKLDGNGELVAVIFGVTGLVGKELAKALLSTGGWKVYGVARRPDNVSPITDTN 60

Query: 61  FQFISCDLLDRSSAHRNLSPVRHVTHVFWVTWAAQLRFDTSDCSDQNRTMLANVLDVILP 120
           FQFISCDLLDRSSAHRNLSPVRHVTHVFWVTWAAQLRFDTSDCSDQNRTMLANVLDVILP
Sbjct: 61  FQFISCDLLDRSSAHRNLSPVRHVTHVFWVTWAAQLRFDTSDCSDQNRTMLANVLDVILP 120

Query: 121 AAPALRHVSLQTGIKHYVSLTGAAVGGGGGRMAVYCEDSPRAESGNNFYYALEDLLRERL 180
           AAPALRHVSLQTGIKHYVSLTGAAVGGGGGRMAVYCEDSPRAESGNNFYYALEDLLRERL
Sbjct: 121 AAPALRHVSLQTGIKHYVSLTGAAVGGGGGRMAVYCEDSPRAESGNNFYYALEDLLRERL 180

Query: 181 SGGGRRVAWSVLRPGLILGCSNRTFFNFMGSLCVYGAICKKLKKPFVFGGSAACWEGFCI 240
           SGGGRRVAWSVLRPGLILGCSNRTFFNFMGSLCVYGAICKKLKKPFVFGGSAACWEGFCI
Sbjct: 181 SGGGRRVAWSVLRPGLILGCSNRTFFNFMGSLCVYGAICKKLKKPFVFGGSAACWEGFCI 240

Query: 241 NGSDARLVAEQHVWAATNEINTTADGEAFNVCNGWSFTWKEIWGAVAEKFGVAAAAEDEE 300
           NGSDARLVAEQHVWAATNEINTTADGEAFNVCNGWSFTWKEIWGAVAEKFGVAAAAEDEE
Sbjct: 241 NGSDARLVAEQHVWAATNEINTTADGEAFNVCNGWSFTWKEIWGAVAEKFGVAAAAEDEE 300

Query: 301 GMFSPEFHYTAAMTEKGRLWAEIVEEEGLIPTEMEELANWGFLDSLFRLPGKMVASRNKS 360
           GMFSPEFHYTAAMTEKGRLWAEIVEEEGLIPTEMEELANWGFLDSLFRLPGKMVASRNKS
Sbjct: 301 GMFSPEFHYTAAMTEKGRLWAEIVEEEGLIPTEMEELANWGFLDSLFRLPGKMVASRNKS 360

Query: 361 DRLGFSVKYKMLDSILYWIDVMRYDKLIPFP 392
           DRLGFSVKYKMLDSILYWIDVMRYDKLIPFP
Sbjct: 361 DRLGFSVKYKMLDSILYWIDVMRYDKLIPFP 391

[Close]

BLAST of CmoCh01G006910 vs. ExPASy TrEMBL
Match: A0A6J1K9J8 (3-oxo-Delta(4,5)-steroid 5-beta-reductase-like OS=Cucurbita maxima OX=3661 GN=LOC111492905 PE=3 SV=1)

HSP 1 Score: 782.7 bits (2020), Expect = 7.1e-223
Identity = 381/393 (96.95%), Postives = 386/393 (98.22%), Query Frame = 0


Query: 1   MAAETINAKLDGNGELVAVIFGVTGLVGKELAKALLSTGGWKVYGVARRPDNVSPITDTN 60
           MAAETINAKLDGNGELVAVIFGVTGLVGKELAK LLSTGGWKVYGVARRPDNVSPITDTN
Sbjct: 1   MAAETINAKLDGNGELVAVIFGVTGLVGKELAKTLLSTGGWKVYGVARRPDNVSPITDTN 60

Query: 61  FQFISCDLLDRSSAHRNLSPVRHVTHVFWVTWAAQLRFDTSDCSDQNRTMLANVLDVILP 120
           FQFISCDLLDRSSAHRNLSPVRHVTH+FWVTW AQLRFDTSDCSDQNRTMLANVLDVILP
Sbjct: 61  FQFISCDLLDRSSAHRNLSPVRHVTHLFWVTWTAQLRFDTSDCSDQNRTMLANVLDVILP 120

Query: 121 AAPALRHVSLQTGIKHYVSLTGAAV-GGGGGRMAVYCEDSPRAESGNNFYYALEDLLRER 180
           AAPALRHVSLQTGIKHYVSLTGAAV GGGGG MAVYCEDSPRAESGNNFYYALEDLLRER
Sbjct: 121 AAPALRHVSLQTGIKHYVSLTGAAVDGGGGGGMAVYCEDSPRAESGNNFYYALEDLLRER 180

Query: 181 LSGGGRRVAWSVLRPGLILGCSNRTFFNFMGSLCVYGAICKKLKKPFVFGGSAACWEGFC 240
           LSGGGRRVAWSVLRPGLILGCSNRTFFNFMGSLCVYGAICKKLKKPFVFGGSAACWEGFC
Sbjct: 181 LSGGGRRVAWSVLRPGLILGCSNRTFFNFMGSLCVYGAICKKLKKPFVFGGSAACWEGFC 240

Query: 241 INGSDARLVAEQHVWAATNEINTTADGEAFNVCNGWSFTWKEIWGAVAEKFGV-AAAAED 300
           INGSDARLVAEQHVWAATNEINTTADGEAFNVCNGWSFTWKEIWGAVAEKFG+ AAAAED
Sbjct: 241 INGSDARLVAEQHVWAATNEINTTADGEAFNVCNGWSFTWKEIWGAVAEKFGLAAAAAED 300

Query: 301 EEGMFSPEFHYTAAMTEKGRLWAEIVEEEGLIPTEMEELANWGFLDSLFRLPGKMVASRN 360
           E+GMFSPEFHYTAAM EKG++WAEIVEEEGLIPTEMEELANWGFLDSLFRLPGKMVASRN
Sbjct: 301 EKGMFSPEFHYTAAMAEKGKVWAEIVEEEGLIPTEMEELANWGFLDSLFRLPGKMVASRN 360

Query: 361 KSDRLGFSVKYKMLDSILYWIDVMRYDKLIPFP 392
           KSDRLGFSVKYKMLDSILYWIDVMRYD LIPFP
Sbjct: 361 KSDRLGFSVKYKMLDSILYWIDVMRYDTLIPFP 393

[Close]

BLAST of CmoCh01G006910 vs. ExPASy TrEMBL
Match: A0A6J1I0N6 (3-oxo-Delta(4,5)-steroid 5-beta-reductase-like OS=Cucurbita maxima OX=3661 GN=LOC111469437 PE=3 SV=1)

HSP 1 Score: 652.5 bits (1682), Expect = 1.1e-183
Identity = 320/393 (81.42%), Postives = 338/393 (86.01%), Query Frame = 0


Query: 1   MAAETINAKLDGNGELVAVIFGVTGLVGKELAKALLSTGGWKVYGVARRPDNVSPITDTN 60
           MA E++N  LD NGELVAVIFGVTGLVGKELAK LLST GWKVYGVARRPDN+SPI+D N
Sbjct: 1   MADESMNTNLDANGELVAVIFGVTGLVGKELAKMLLSTAGWKVYGVARRPDNISPISDPN 60

Query: 61  FQFISCDLLDRSSAHRNLSPVRHVTHVFWVTWAAQLRFDTSDCSDQNRTMLANVLDVILP 120
           F FISCDLLDR SAH NLSPVRHVTH+FW+TWAAQLRFD+ DCSDQN+ MLAN LD ILP
Sbjct: 61  FHFISCDLLDRRSAHLNLSPVRHVTHMFWITWAAQLRFDSQDCSDQNKAMLANALDAILP 120

Query: 121 AAPALRHVSLQTGIKHYVSLTGAAVGGGGGRMAVYCEDSPRAESGNNFYYALEDLLRERL 180
           +A ALRHVSLQTG+KHY SLT +   GGG    VY EDSPRAESGNNFYY LEDLLRERL
Sbjct: 121 SASALRHVSLQTGVKHYASLTSSTSSGGG--EVVYSEDSPRAESGNNFYYVLEDLLRERL 180

Query: 181 SGGGRRVAWSVLRPGLILGCSNRTFFNFMGSLCVYGAICKKLKKPFVFGGSAACWEGFCI 240
           SG  R VAWS+LRPGLILGCSNRTFFNFMGSLCVYGAICKKLK PFVFGG+AACWE   I
Sbjct: 181 SGYRRVVAWSILRPGLILGCSNRTFFNFMGSLCVYGAICKKLKMPFVFGGTAACWEEVYI 240

Query: 241 NGSDARLVAEQHVWAATN--EINTTADGEAFNVCNGWSFTWKEIWGAVAEKFGVAAAAED 300
           +GSDARL AEQHVWAAT   EIN   D EAFNVCNGWSFTWKEIWGAVAEK GV   AED
Sbjct: 241 DGSDARLTAEQHVWAATQAVEINAITDREAFNVCNGWSFTWKEIWGAVAEKLGVTVGAED 300

Query: 301 EEGMFSPEFHYTAAMTEKGRLWAEIVEEEGLIPTEMEELANWGFLDSLFRLPGKMVASRN 360
           E  MFSPEFHYT AM EKG +WAEIVEEEGL+ TEM ELANWGFLDSLFRLP KMVASR 
Sbjct: 301 ER-MFSPEFHYTEAMAEKGMVWAEIVEEEGLVATEMGELANWGFLDSLFRLPEKMVASRA 360

Query: 361 KSDRLGFSVKYKMLDSILYWIDVMRYDKLIPFP 392
           KSDRLGF+VKYKMLDSILYWIDVMR+DKLIP P
Sbjct: 361 KSDRLGFTVKYKMLDSILYWIDVMRHDKLIPSP 390

[Close]

BLAST of CmoCh01G006910 vs. ExPASy TrEMBL
Match: A0A0A0LVI2 (Epimerase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G530150 PE=3 SV=1)

HSP 1 Score: 650.2 bits (1676), Expect = 5.5e-183
Identity = 320/395 (81.01%), Postives = 339/395 (85.82%), Query Frame = 0


Query: 1   MAAETINAKLDGNGELVAVIFGVTGLVGKELAKALLSTGGWKVYGVARRPDNVSPITDTN 60
           MA E IN  LD NGELVAVIFGVTGLVGK+LAK LLST GWKVYGVARRPDNVSPI+   
Sbjct: 1   MAVEIINTNLDANGELVAVIFGVTGLVGKQLAKTLLSTAGWKVYGVARRPDNVSPISHPK 60

Query: 61  FQFISCDLLDRSSAHRNLSPVRHVTHVFWVTWAAQLRFDTSDCSDQNRTMLANVLDVILP 120
           F FISCDLLDR S  +NLSPVRHVTH+FW+TWAAQLR D+ DCSDQNR MLAN LD ILP
Sbjct: 61  FHFISCDLLDRRSVQQNLSPVRHVTHLFWITWAAQLRLDSPDCSDQNRAMLANALDAILP 120

Query: 121 AAPALRHVSLQTGIKHYVSLT--GAAVGGGGGRMAVYCEDSPRAESGNNFYYALEDLLRE 180
           +APALRHVSLQTGIKHY SLT   A  GGGG    VYCEDSPRAESGNNFYY LEDLLRE
Sbjct: 121 SAPALRHVSLQTGIKHYASLTRLAAGCGGGGEGEVVYCEDSPRAESGNNFYYVLEDLLRE 180

Query: 181 RLSGGGRRVAWSVLRPGLILGCSNRTFFNFMGSLCVYGAICKKLKKPFVFGGSAACWEGF 240
           RLS G R VAWSVLRPGLILGCSNRTFFNFMGSLCVYGAICKKLK PFVFGG+AACWE  
Sbjct: 181 RLSCGRRMVAWSVLRPGLILGCSNRTFFNFMGSLCVYGAICKKLKMPFVFGGTAACWEEV 240

Query: 241 CINGSDARLVAEQHVWAATN--EINTTADGEAFNVCNGWSFTWKEIWGAVAEKFGVA--A 300
            I+GSDARL AEQH+W AT   EIN TADGEAFNVCNGWSF WKEIWGAVAEK GVA   
Sbjct: 241 YIDGSDARLTAEQHIWVATKAVEINATADGEAFNVCNGWSFRWKEIWGAVAEKLGVATTG 300

Query: 301 AAEDEEGMFSPEFHYTAAMTEKGRLWAEIVEEEGLIPTEMEELANWGFLDSLFRLPGKMV 360
           A E++E MFS EFHYTAAM +KG++WAEIVEEEGL+ TEMEELANWGFLD+LFRLP KMV
Sbjct: 301 AEEEKERMFSKEFHYTAAMGDKGKVWAEIVEEEGLVTTEMEELANWGFLDTLFRLPEKMV 360

Query: 361 ASRNKSDRLGFSVKYKMLDSILYWIDVMRYDKLIP 390
           ASR KSDRLGF+VKYKMLDS+LYWIDVMR DKLIP
Sbjct: 361 ASRAKSDRLGFNVKYKMLDSMLYWIDVMRNDKLIP 395

[Close]

BLAST of CmoCh01G006910 vs. ExPASy TrEMBL
Match: A0A6J1HM54 (3-oxo-Delta(4,5)-steroid 5-beta-reductase-like OS=Cucurbita moschata OX=3662 GN=LOC111464842 PE=3 SV=1)

HSP 1 Score: 650.2 bits (1676), Expect = 5.5e-183
Identity = 319/393 (81.17%), Postives = 341/393 (86.77%), Query Frame = 0


Query: 1   MAAETINAKLDGNGELVAVIFGVTGLVGKELAKALLSTGGWKVYGVARRPDNVSPITDTN 60
           MA E++N  LD NGELVAVIFGVTGLVGKELAK LLST GWKVYGVARRPDN+SPI+D N
Sbjct: 1   MADESMNTNLDANGELVAVIFGVTGLVGKELAKMLLSTAGWKVYGVARRPDNISPISDPN 60

Query: 61  FQFISCDLLDRSSAHRNLSPVRHVTHVFWVTWAAQLRFDTSDCSDQNRTMLANVLDVILP 120
           F FISCDLLDR SAH NLSP+ HVTH+FW+TWAAQLRFD+ DCSDQN+ MLAN LD ILP
Sbjct: 61  FHFISCDLLDRRSAHLNLSPLGHVTHMFWITWAAQLRFDSQDCSDQNKAMLANALDAILP 120

Query: 121 AAPALRHVSLQTGIKHYVSLTGAAVGGGGGRMAVYCEDSPRAESGNNFYYALEDLLRERL 180
           +APALRHVSLQTG+KHY SLT ++ G GGG + VY E+SPRAESGNNFYY LEDLLRERL
Sbjct: 121 SAPALRHVSLQTGVKHYASLT-SSTGSGGGEV-VYSENSPRAESGNNFYYVLEDLLRERL 180

Query: 181 SGGGRRVAWSVLRPGLILGCSNRTFFNFMGSLCVYGAICKKLKKPFVFGGSAACWEGFCI 240
           SG  R VAWS+LRPGLILGCSNRTFFNFMGSLCVYGAICKKLK PFVFGG+AACWE   I
Sbjct: 181 SGRRRVVAWSILRPGLILGCSNRTFFNFMGSLCVYGAICKKLKMPFVFGGTAACWEEVYI 240

Query: 241 NGSDARLVAEQHVWAATN--EINTTADGEAFNVCNGWSFTWKEIWGAVAEKFGVAAAAED 300
           + SDARL AEQHVWAAT   EIN   DGEAFNVCNGWSFTWKEIWGAVAEK GV   AED
Sbjct: 241 DSSDARLTAEQHVWAATKAVEINALTDGEAFNVCNGWSFTWKEIWGAVAEKLGVTVGAED 300

Query: 301 EEGMFSPEFHYTAAMTEKGRLWAEIVEEEGLIPTEMEELANWGFLDSLFRLPGKMVASRN 360
           E  MFSPEF YTAAM EKG +WAEIVEEEGL+ TEM ELANWGFLDSLFRLP KMVASR 
Sbjct: 301 ER-MFSPEFRYTAAMAEKGTVWAEIVEEEGLVATEMGELANWGFLDSLFRLPEKMVASRA 360

Query: 361 KSDRLGFSVKYKMLDSILYWIDVMRYDKLIPFP 392
           KSDRLGF+VKYKMLDSILYWIDVMR+DKLIP P
Sbjct: 361 KSDRLGFTVKYKMLDSILYWIDVMRHDKLIPSP 390

[Close]

BLAST of CmoCh01G006910 vs. NCBI nr
Match: XP_022948773.1 (3-oxo-Delta(4,5)-steroid 5-beta-reductase-like [Cucurbita moschata])

HSP 1 Score: 808.1 bits (2086), Expect = 3.2e-230
Identity = 391/391 (100.00%), Postives = 391/391 (100.00%), Query Frame = 0


Query: 1   MAAETINAKLDGNGELVAVIFGVTGLVGKELAKALLSTGGWKVYGVARRPDNVSPITDTN 60
           MAAETINAKLDGNGELVAVIFGVTGLVGKELAKALLSTGGWKVYGVARRPDNVSPITDTN
Sbjct: 1   MAAETINAKLDGNGELVAVIFGVTGLVGKELAKALLSTGGWKVYGVARRPDNVSPITDTN 60

Query: 61  FQFISCDLLDRSSAHRNLSPVRHVTHVFWVTWAAQLRFDTSDCSDQNRTMLANVLDVILP 120
           FQFISCDLLDRSSAHRNLSPVRHVTHVFWVTWAAQLRFDTSDCSDQNRTMLANVLDVILP
Sbjct: 61  FQFISCDLLDRSSAHRNLSPVRHVTHVFWVTWAAQLRFDTSDCSDQNRTMLANVLDVILP 120

Query: 121 AAPALRHVSLQTGIKHYVSLTGAAVGGGGGRMAVYCEDSPRAESGNNFYYALEDLLRERL 180
           AAPALRHVSLQTGIKHYVSLTGAAVGGGGGRMAVYCEDSPRAESGNNFYYALEDLLRERL
Sbjct: 121 AAPALRHVSLQTGIKHYVSLTGAAVGGGGGRMAVYCEDSPRAESGNNFYYALEDLLRERL 180

Query: 181 SGGGRRVAWSVLRPGLILGCSNRTFFNFMGSLCVYGAICKKLKKPFVFGGSAACWEGFCI 240
           SGGGRRVAWSVLRPGLILGCSNRTFFNFMGSLCVYGAICKKLKKPFVFGGSAACWEGFCI
Sbjct: 181 SGGGRRVAWSVLRPGLILGCSNRTFFNFMGSLCVYGAICKKLKKPFVFGGSAACWEGFCI 240

Query: 241 NGSDARLVAEQHVWAATNEINTTADGEAFNVCNGWSFTWKEIWGAVAEKFGVAAAAEDEE 300
           NGSDARLVAEQHVWAATNEINTTADGEAFNVCNGWSFTWKEIWGAVAEKFGVAAAAEDEE
Sbjct: 241 NGSDARLVAEQHVWAATNEINTTADGEAFNVCNGWSFTWKEIWGAVAEKFGVAAAAEDEE 300

Query: 301 GMFSPEFHYTAAMTEKGRLWAEIVEEEGLIPTEMEELANWGFLDSLFRLPGKMVASRNKS 360
           GMFSPEFHYTAAMTEKGRLWAEIVEEEGLIPTEMEELANWGFLDSLFRLPGKMVASRNKS
Sbjct: 301 GMFSPEFHYTAAMTEKGRLWAEIVEEEGLIPTEMEELANWGFLDSLFRLPGKMVASRNKS 360

Query: 361 DRLGFSVKYKMLDSILYWIDVMRYDKLIPFP 392
           DRLGFSVKYKMLDSILYWIDVMRYDKLIPFP
Sbjct: 361 DRLGFSVKYKMLDSILYWIDVMRYDKLIPFP 391

[Close]

BLAST of CmoCh01G006910 vs. NCBI nr
Match: KAG6607304.1 ((S)-8-oxocitronellyl enol synthase CYC2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 801.6 bits (2069), Expect = 3.0e-228
Identity = 387/391 (98.98%), Postives = 390/391 (99.74%), Query Frame = 0


Query: 1   MAAETINAKLDGNGELVAVIFGVTGLVGKELAKALLSTGGWKVYGVARRPDNVSPITDTN 60
           M AETINAKLDGNGELVAVIFGVTGLVGKELAKALLSTGGWKVYGVARRPDNVSPITDTN
Sbjct: 1   MTAETINAKLDGNGELVAVIFGVTGLVGKELAKALLSTGGWKVYGVARRPDNVSPITDTN 60

Query: 61  FQFISCDLLDRSSAHRNLSPVRHVTHVFWVTWAAQLRFDTSDCSDQNRTMLANVLDVILP 120
           FQFISCDLLDRSSA+RNLSPVRHVTH+FWVTWAAQLRFDTSDCSDQNRTMLANVLDVILP
Sbjct: 61  FQFISCDLLDRSSANRNLSPVRHVTHLFWVTWAAQLRFDTSDCSDQNRTMLANVLDVILP 120

Query: 121 AAPALRHVSLQTGIKHYVSLTGAAVGGGGGRMAVYCEDSPRAESGNNFYYALEDLLRERL 180
           AAPALRHVSLQTGIKHYVSLTGAAVGGGGGRMAVYCEDSPRAESGNNFYYALEDLLRERL
Sbjct: 121 AAPALRHVSLQTGIKHYVSLTGAAVGGGGGRMAVYCEDSPRAESGNNFYYALEDLLRERL 180

Query: 181 SGGGRRVAWSVLRPGLILGCSNRTFFNFMGSLCVYGAICKKLKKPFVFGGSAACWEGFCI 240
           SGGGRRVAWSVLRPGLILGCSNRTFFNFMGSLCVYGAICKKLKKPFVFGGSAACWEGFCI
Sbjct: 181 SGGGRRVAWSVLRPGLILGCSNRTFFNFMGSLCVYGAICKKLKKPFVFGGSAACWEGFCI 240

Query: 241 NGSDARLVAEQHVWAATNEINTTADGEAFNVCNGWSFTWKEIWGAVAEKFGVAAAAEDEE 300
           NGSDARLVAEQHVWAATNEINTTADGEAFNVCNGWSFTWKEIWGAVAEKFGVAAAAEDEE
Sbjct: 241 NGSDARLVAEQHVWAATNEINTTADGEAFNVCNGWSFTWKEIWGAVAEKFGVAAAAEDEE 300

Query: 301 GMFSPEFHYTAAMTEKGRLWAEIVEEEGLIPTEMEELANWGFLDSLFRLPGKMVASRNKS 360
           GMFSPEFHYTAAMTEKGR+WAEIVEEEGLIPTEMEELANWGFLDSLFRLPGKMVASRNKS
Sbjct: 301 GMFSPEFHYTAAMTEKGRVWAEIVEEEGLIPTEMEELANWGFLDSLFRLPGKMVASRNKS 360

Query: 361 DRLGFSVKYKMLDSILYWIDVMRYDKLIPFP 392
           DRLGFSVKYKMLDSILYWIDVMRYDKLIPFP
Sbjct: 361 DRLGFSVKYKMLDSILYWIDVMRYDKLIPFP 391

[Close]

BLAST of CmoCh01G006910 vs. NCBI nr
Match: KAG7036983.1 (3-oxo-Delta(4,5)-steroid 5-beta-reductase, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 800.8 bits (2067), Expect = 5.2e-228
Identity = 387/391 (98.98%), Postives = 390/391 (99.74%), Query Frame = 0


Query: 1   MAAETINAKLDGNGELVAVIFGVTGLVGKELAKALLSTGGWKVYGVARRPDNVSPITDTN 60
           MAAETINAKLDGNGELVAVIFGVTGLVGKELAKALLSTGGWKVYGVARRPDNVSPITDTN
Sbjct: 1   MAAETINAKLDGNGELVAVIFGVTGLVGKELAKALLSTGGWKVYGVARRPDNVSPITDTN 60

Query: 61  FQFISCDLLDRSSAHRNLSPVRHVTHVFWVTWAAQLRFDTSDCSDQNRTMLANVLDVILP 120
           FQFISCDLLDRSSA+RNLSPVRHVTH+FWVTWAAQLRFDTSDCSDQNRTMLANVLDVILP
Sbjct: 61  FQFISCDLLDRSSANRNLSPVRHVTHLFWVTWAAQLRFDTSDCSDQNRTMLANVLDVILP 120

Query: 121 AAPALRHVSLQTGIKHYVSLTGAAVGGGGGRMAVYCEDSPRAESGNNFYYALEDLLRERL 180
           AAPALRHVSLQTGIKHYVSLTGAAVGGGGGRMAVYCEDSPRAESGNNFYYALEDLLRERL
Sbjct: 121 AAPALRHVSLQTGIKHYVSLTGAAVGGGGGRMAVYCEDSPRAESGNNFYYALEDLLRERL 180

Query: 181 SGGGRRVAWSVLRPGLILGCSNRTFFNFMGSLCVYGAICKKLKKPFVFGGSAACWEGFCI 240
           SGGGRRVAWSVLRPGLILGCSNRTFFNFMGSLCVYGAICKKLKKPFVFGGSAACWEGFCI
Sbjct: 181 SGGGRRVAWSVLRPGLILGCSNRTFFNFMGSLCVYGAICKKLKKPFVFGGSAACWEGFCI 240

Query: 241 NGSDARLVAEQHVWAATNEINTTADGEAFNVCNGWSFTWKEIWGAVAEKFGVAAAAEDEE 300
           NGSDARLVAEQHVWAATNEINTTADGEAFNVCNGWSFTWKEIWGAVAEKFGVAAAAEDEE
Sbjct: 241 NGSDARLVAEQHVWAATNEINTTADGEAFNVCNGWSFTWKEIWGAVAEKFGVAAAAEDEE 300

Query: 301 GMFSPEFHYTAAMTEKGRLWAEIVEEEGLIPTEMEELANWGFLDSLFRLPGKMVASRNKS 360
           GMFSPEFHY AAMTEKGR+WAEIVEEEGLIPTEMEELANWGFLDSLFRLPGKMVASRNKS
Sbjct: 301 GMFSPEFHYMAAMTEKGRVWAEIVEEEGLIPTEMEELANWGFLDSLFRLPGKMVASRNKS 360

Query: 361 DRLGFSVKYKMLDSILYWIDVMRYDKLIPFP 392
           DRLGFSVKYKMLDSILYWIDVMRYDKLIPFP
Sbjct: 361 DRLGFSVKYKMLDSILYWIDVMRYDKLIPFP 391

[Close]

BLAST of CmoCh01G006910 vs. NCBI nr
Match: XP_022998181.1 (3-oxo-Delta(4,5)-steroid 5-beta-reductase-like [Cucurbita maxima])

HSP 1 Score: 782.7 bits (2020), Expect = 1.5e-222
Identity = 381/393 (96.95%), Postives = 386/393 (98.22%), Query Frame = 0


Query: 1   MAAETINAKLDGNGELVAVIFGVTGLVGKELAKALLSTGGWKVYGVARRPDNVSPITDTN 60
           MAAETINAKLDGNGELVAVIFGVTGLVGKELAK LLSTGGWKVYGVARRPDNVSPITDTN
Sbjct: 1   MAAETINAKLDGNGELVAVIFGVTGLVGKELAKTLLSTGGWKVYGVARRPDNVSPITDTN 60

Query: 61  FQFISCDLLDRSSAHRNLSPVRHVTHVFWVTWAAQLRFDTSDCSDQNRTMLANVLDVILP 120
           FQFISCDLLDRSSAHRNLSPVRHVTH+FWVTW AQLRFDTSDCSDQNRTMLANVLDVILP
Sbjct: 61  FQFISCDLLDRSSAHRNLSPVRHVTHLFWVTWTAQLRFDTSDCSDQNRTMLANVLDVILP 120

Query: 121 AAPALRHVSLQTGIKHYVSLTGAAV-GGGGGRMAVYCEDSPRAESGNNFYYALEDLLRER 180
           AAPALRHVSLQTGIKHYVSLTGAAV GGGGG MAVYCEDSPRAESGNNFYYALEDLLRER
Sbjct: 121 AAPALRHVSLQTGIKHYVSLTGAAVDGGGGGGMAVYCEDSPRAESGNNFYYALEDLLRER 180

Query: 181 LSGGGRRVAWSVLRPGLILGCSNRTFFNFMGSLCVYGAICKKLKKPFVFGGSAACWEGFC 240
           LSGGGRRVAWSVLRPGLILGCSNRTFFNFMGSLCVYGAICKKLKKPFVFGGSAACWEGFC
Sbjct: 181 LSGGGRRVAWSVLRPGLILGCSNRTFFNFMGSLCVYGAICKKLKKPFVFGGSAACWEGFC 240

Query: 241 INGSDARLVAEQHVWAATNEINTTADGEAFNVCNGWSFTWKEIWGAVAEKFGV-AAAAED 300
           INGSDARLVAEQHVWAATNEINTTADGEAFNVCNGWSFTWKEIWGAVAEKFG+ AAAAED
Sbjct: 241 INGSDARLVAEQHVWAATNEINTTADGEAFNVCNGWSFTWKEIWGAVAEKFGLAAAAAED 300

Query: 301 EEGMFSPEFHYTAAMTEKGRLWAEIVEEEGLIPTEMEELANWGFLDSLFRLPGKMVASRN 360
           E+GMFSPEFHYTAAM EKG++WAEIVEEEGLIPTEMEELANWGFLDSLFRLPGKMVASRN
Sbjct: 301 EKGMFSPEFHYTAAMAEKGKVWAEIVEEEGLIPTEMEELANWGFLDSLFRLPGKMVASRN 360

Query: 361 KSDRLGFSVKYKMLDSILYWIDVMRYDKLIPFP 392
           KSDRLGFSVKYKMLDSILYWIDVMRYD LIPFP
Sbjct: 361 KSDRLGFSVKYKMLDSILYWIDVMRYDTLIPFP 393

[Close]

BLAST of CmoCh01G006910 vs. NCBI nr
Match: XP_023525980.1 (3-oxo-Delta(4,5)-steroid 5-beta-reductase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 770.8 bits (1989), Expect = 5.7e-219
Identity = 375/391 (95.91%), Postives = 381/391 (97.44%), Query Frame = 0


Query: 1   MAAETINAKLDGNGELVAVIFGVTGLVGKELAKALLSTGGWKVYGVARRPDNVSPITDTN 60
           MAAETINAKLDGNGE VAVIFGVTGLVGKELAK LLSTGGWKVYGVARRPDNVSPITD+N
Sbjct: 1   MAAETINAKLDGNGEPVAVIFGVTGLVGKELAKTLLSTGGWKVYGVARRPDNVSPITDSN 60

Query: 61  FQFISCDLLDRSSAHRNLSPVRHVTHVFWVTWAAQLRFDTSDCSDQNRTMLANVLDVILP 120
           FQFISCDLLDRSSAHRNLSPVRHVTH+FWVTWAAQLRFDTSDCSDQNRTMLANVLDVILP
Sbjct: 61  FQFISCDLLDRSSAHRNLSPVRHVTHLFWVTWAAQLRFDTSDCSDQNRTMLANVLDVILP 120

Query: 121 AAPALRHVSLQTGIKHYVSLTGAAVGGGGGRMAVYCEDSPRAESGNNFYYALEDLLRERL 180
           AA ALRHVSLQTGIKHYVSLT    GGGGGRMAVYCEDSPRAESGNNFYYALEDLLRERL
Sbjct: 121 AASALRHVSLQTGIKHYVSLT----GGGGGRMAVYCEDSPRAESGNNFYYALEDLLRERL 180

Query: 181 SGGGRRVAWSVLRPGLILGCSNRTFFNFMGSLCVYGAICKKLKKPFVFGGSAACWEGFCI 240
           SGGGRRVAWSVLRPGLILGCSNRTFFNFMGSLCVYGAICKKLKKPFVFGGSAACWEGFCI
Sbjct: 181 SGGGRRVAWSVLRPGLILGCSNRTFFNFMGSLCVYGAICKKLKKPFVFGGSAACWEGFCI 240

Query: 241 NGSDARLVAEQHVWAATNEINTTADGEAFNVCNGWSFTWKEIWGAVAEKFGVAAAAEDEE 300
           NGSDARLVAEQHVWAATNEINTTADGEAFNVCNGWSFTWKEIWGAVAEKFGV  AAEDEE
Sbjct: 241 NGSDARLVAEQHVWAATNEINTTADGEAFNVCNGWSFTWKEIWGAVAEKFGV--AAEDEE 300

Query: 301 GMFSPEFHYTAAMTEKGRLWAEIVEEEGLIPTEMEELANWGFLDSLFRLPGKMVASRNKS 360
           GMFSPEFHYTAAM EKG++WAEIVEEEGLIPT+MEELANWGFLDSLFRLPGKMVASRNKS
Sbjct: 301 GMFSPEFHYTAAMAEKGKVWAEIVEEEGLIPTDMEELANWGFLDSLFRLPGKMVASRNKS 360

Query: 361 DRLGFSVKYKMLDSILYWIDVMRYDKLIPFP 392
           DRLGFSVKYKMLDSILYWIDVMR+DKLIPFP
Sbjct: 361 DRLGFSVKYKMLDSILYWIDVMRHDKLIPFP 385

[Close]

BLAST of CmoCh01G006910 vs. TAIR 10
Match: AT5G58750.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 401.7 bits (1031), Expect = 6.5e-112
Identity = 206/378 (54.50%), Postives = 261/378 (69.05%), Query Frame = 0


Query: 15  ELVAVIFGVTGLVGKELAKALL-STGGWKVYGVARRPDNVSPITDTNFQFISCDLLDRSS 74
           E VA+IFGVTGLVG+E+ K LL S  GW++YGVAR P+ ++ +T   + FISCDLL+ S 
Sbjct: 16  ENVALIFGVTGLVGREIVKTLLMSKPGWRIYGVARNPE-INSMTKM-YNFISCDLLNASE 75

Query: 75  AHRNLSPVRH-VTHVFWVTWAAQLRFDTSDCSDQNRTMLANVLDVILPAAPALRHVSLQT 134
             + LSP++  V+HVFWVTW+ +   DT +C  QN+TML N LD ILP A  L+H SLQT
Sbjct: 76  TKQRLSPLQDIVSHVFWVTWSGEFPLDTDECCVQNKTMLMNALDAILPNAKRLKHFSLQT 135

Query: 135 GIKHYVSLTGAAVG-GGGGRMAVYCEDSPRAESGNNFYYALEDLLRERLSGGGRRVAWSV 194
           G+KHYVSL    +  G G  +  Y E+ PR  SG NFYY LEDLL+E+++     V WSV
Sbjct: 136 GMKHYVSLVEETMARGEGSSLYYYSEECPRKSSGKNFYYVLEDLLKEKITRSS--VVWSV 195

Query: 195 LRPGLILGCSNRTFFNFMGSLCVYGAICKKLKKPFVFGGSAACWEGFCINGSDARLVAEQ 254
            RPGL++G S+RT +NFMGSLCVYGA+CK L  PFVFGG+  CWE   I+GSD+ LVAEQ
Sbjct: 196 QRPGLLMGSSSRTLYNFMGSLCVYGAMCKYLNLPFVFGGTRECWEESYIDGSDSNLVAEQ 255

Query: 255 HVWAATNEINTTADGEAFNVCNGWSFTWKEIWGAVAEKFGVAAAAEDEEGMFSPEFHYTA 314
           H++AAT+       GEAFN  NG  FTWKEIW  + +K GV     +E  MF   F +  
Sbjct: 256 HIFAATSG-KVREKGEAFNAINGVGFTWKEIWPEIGKKLGVQV---NETTMFDEGFWFGR 315

Query: 315 AMTEKGRLWAEIVEEEGLIPTEMEELANWGFLDSLFRLPGKMVASRNKSDRLGFSVKYKM 374
            M E+  +W EIV +E L+ TE+E+LANW FLD+LFR P K++  R K DR GF  KY+ 
Sbjct: 316 EMVERKHVWDEIVVKEKLVRTEIEDLANWYFLDALFRCPFKLLGKREKVDRFGFKRKYRT 375

Query: 375 LDSILYWIDVMRYDKLIP 390
           LDS+LYWIDVMR +KLIP
Sbjct: 376 LDSVLYWIDVMRDEKLIP 385

[Close]

BLAST of CmoCh01G006910 vs. TAIR 10
Match: AT4G24220.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 250.4 bits (638), Expect = 2.4e-66
Identity = 156/380 (41.05%), Postives = 203/380 (53.42%), Query Frame = 0


Query: 15  ELVAVIFGVTGLVGKELAKAL----LSTGGWKVYGVARRPDNVSPITDTNFQFISCDLLD 74
           E VA+I GVTG+VG  LA+ L       G WKVYGVARRP   +   D    +I CD+ D
Sbjct: 26  ESVALIIGVTGIVGNSLAEILPLSDTPGGPWKVYGVARRP-RPTWNADHPIDYIQCDVSD 85

Query: 75  RSSAHRNLSPVRHVTHVFWVTWAAQLRFDTSDCSDQNRTMLANVLDVILPAAPALRHVSL 134
                  LSP+  VTHVF+VTW    R   S+  + N +ML NVL  I+P AP LRHV L
Sbjct: 86  AEDTRSKLSPLTDVTHVFYVTWTN--RESESENCEANGSMLRNVLQAIIPYAPNLRHVCL 145

Query: 135 QTGIKHYVSLTGAAVGGGGGRM-AVYCEDSPRAESGNNFYYALEDLLRERLSGGGRRVAW 194
           QTG KHY+   G      G R    + ED PR +   NFYY  ED+L E +      V W
Sbjct: 146 QTGTKHYL---GPFTNVDGPRHDPPFTEDMPRLQI-QNFYYTQEDILFEEIK-KIETVTW 205

Query: 195 SVLRPGLILGCSNRTFFNFMGSLCVYGAICKKLKKPFVFGGSAACWEGFCINGSDARLVA 254
           S+ RP +I G S  +  N +G+LCVY AICK    P +F GS   WEGF +  SDA L+A
Sbjct: 206 SIHRPNMIFGFSPYSLMNIVGTLCVYAAICKHEGSPLLFPGSKKAWEGF-MTASDADLIA 265

Query: 255 EQHVWAATNEINTTADGEAFNVCNGWSFTWKEIWGAVAEKFGVAAAAEDEEGMFSPEFHY 314
           EQ +WAA   ++  A  EAFN  N   F WK +W  +AE+FG+     +E          
Sbjct: 266 EQQIWAA---VDPYAKNEAFNCNNADIFKWKHLWKILAEQFGIEEYGFEE----GKNLGL 325

Query: 315 TAAMTEKGRLWAEIVEEEGLIPTEMEELANWGFLDSLFRLPGKMVASRNKSDRLGFSVKY 374
              M  K R+W E+V+E  L   ++EE+  W F D +  + G M+ S NKS   GF    
Sbjct: 326 VEMMKGKERVWEEMVKENQLQEKKLEEVGVWWFADVILGVEG-MIDSMNKSKEYGFLGFR 385

Query: 375 KMLDSILYWIDVMRYDKLIP 390
              +S + WID  +  K++P
Sbjct: 386 NSNNSFISWIDKYKAFKIVP 388

[Close]

BLAST of CmoCh01G006910 vs. TAIR 10
Match: AT4G24220.2 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 250.4 bits (638), Expect = 2.4e-66
Identity = 156/380 (41.05%), Postives = 203/380 (53.42%), Query Frame = 0


Query: 15  ELVAVIFGVTGLVGKELAKAL----LSTGGWKVYGVARRPDNVSPITDTNFQFISCDLLD 74
           E VA+I GVTG+VG  LA+ L       G WKVYGVARRP   +   D    +I CD+ D
Sbjct: 25  ESVALIIGVTGIVGNSLAEILPLSDTPGGPWKVYGVARRP-RPTWNADHPIDYIQCDVSD 84

Query: 75  RSSAHRNLSPVRHVTHVFWVTWAAQLRFDTSDCSDQNRTMLANVLDVILPAAPALRHVSL 134
                  LSP+  VTHVF+VTW    R   S+  + N +ML NVL  I+P AP LRHV L
Sbjct: 85  AEDTRSKLSPLTDVTHVFYVTWTN--RESESENCEANGSMLRNVLQAIIPYAPNLRHVCL 144

Query: 135 QTGIKHYVSLTGAAVGGGGGRM-AVYCEDSPRAESGNNFYYALEDLLRERLSGGGRRVAW 194
           QTG KHY+   G      G R    + ED PR +   NFYY  ED+L E +      V W
Sbjct: 145 QTGTKHYL---GPFTNVDGPRHDPPFTEDMPRLQI-QNFYYTQEDILFEEIK-KIETVTW 204

Query: 195 SVLRPGLILGCSNRTFFNFMGSLCVYGAICKKLKKPFVFGGSAACWEGFCINGSDARLVA 254
           S+ RP +I G S  +  N +G+LCVY AICK    P +F GS   WEGF +  SDA L+A
Sbjct: 205 SIHRPNMIFGFSPYSLMNIVGTLCVYAAICKHEGSPLLFPGSKKAWEGF-MTASDADLIA 264

Query: 255 EQHVWAATNEINTTADGEAFNVCNGWSFTWKEIWGAVAEKFGVAAAAEDEEGMFSPEFHY 314
           EQ +WAA   ++  A  EAFN  N   F WK +W  +AE+FG+     +E          
Sbjct: 265 EQQIWAA---VDPYAKNEAFNCNNADIFKWKHLWKILAEQFGIEEYGFEE----GKNLGL 324

Query: 315 TAAMTEKGRLWAEIVEEEGLIPTEMEELANWGFLDSLFRLPGKMVASRNKSDRLGFSVKY 374
              M  K R+W E+V+E  L   ++EE+  W F D +  + G M+ S NKS   GF    
Sbjct: 325 VEMMKGKERVWEEMVKENQLQEKKLEEVGVWWFADVILGVEG-MIDSMNKSKEYGFLGFR 384

Query: 375 KMLDSILYWIDVMRYDKLIP 390
              +S + WID  +  K++P
Sbjct: 385 NSNNSFISWIDKYKAFKIVP 387

The following BLAST results are available for this feature:

BLAST of CmoCh01G006910 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
A0A1C9CX66	1.1e-66	42.06	(S)-8-oxocitronellyl enol synthase CYC2 OS=Camptotheca acuminata OX=16922 GN=CYC...	[more]
Q9STX2	3.4e-65	41.05	3-oxo-Delta(4,5)-steroid 5-beta-reductase OS=Arabidopsis thaliana OX=3702 GN=VEP...	[more]
Q6PQJ9	2.7e-62	40.05	3-oxo-Delta(4,5)-steroid 5-beta-reductase OS=Digitalis lanata OX=49450 PE=1 SV=1	[more]
A0A221J5X3	7.9e-62	40.84	(S)-8-oxocitronellyl enol synthase ISY1 OS=Nepeta racemosa OX=54731 GN=ISY1 PE=1...	[more]
A0A221J5X1	1.0e-61	38.85	(S)-8-oxocitronellyl enol synthase ISY2 OS=Nepeta cataria OX=39347 GN=ISY2 PE=1 ...	[more]

BLAST of CmoCh01G006910 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1GA93	1.6e-230	100.00	3-oxo-Delta(4,5)-steroid 5-beta-reductase-like OS=Cucurbita moschata OX=3662 GN=...	[more]
A0A6J1K9J8	7.1e-223	96.95	3-oxo-Delta(4,5)-steroid 5-beta-reductase-like OS=Cucurbita maxima OX=3661 GN=LO...	[more]
A0A6J1I0N6	1.1e-183	81.42	3-oxo-Delta(4,5)-steroid 5-beta-reductase-like OS=Cucurbita maxima OX=3661 GN=LO...	[more]
A0A0A0LVI2	5.5e-183	81.01	Epimerase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G530150 P...	[more]
A0A6J1HM54	5.5e-183	81.17	3-oxo-Delta(4,5)-steroid 5-beta-reductase-like OS=Cucurbita moschata OX=3662 GN=...	[more]

BLAST of CmoCh01G006910 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022948773.1	3.2e-230	100.00	3-oxo-Delta(4,5)-steroid 5-beta-reductase-like [Cucurbita moschata]	[more]
KAG6607304.1	3.0e-228	98.98	(S)-8-oxocitronellyl enol synthase CYC2, partial [Cucurbita argyrosperma subsp. ...	[more]
KAG7036983.1	5.2e-228	98.98	3-oxo-Delta(4,5)-steroid 5-beta-reductase, partial [Cucurbita argyrosperma subsp...	[more]
XP_022998181.1	1.5e-222	96.95	3-oxo-Delta(4,5)-steroid 5-beta-reductase-like [Cucurbita maxima]	[more]
XP_023525980.1	5.7e-219	95.91	3-oxo-Delta(4,5)-steroid 5-beta-reductase-like [Cucurbita pepo subsp. pepo]	[more]

BLAST of CmoCh01G006910 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G58750.1	6.5e-112	54.50	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT4G24220.1	2.4e-66	41.05	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT4G24220.2	2.4e-66	41.05	NAD(P)-binding Rossmann-fold superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001509	NAD-dependent epimerase/dehydratase	PFAM	PF01370	Epimerase	coord: 19..119 e-value: 2.9E-8 score: 33.5
None	No IPR available	GENE3D	3.40.50.720		coord: 16..389 e-value: 9.3E-102 score: 342.6
None	No IPR available	PANTHER	PTHR32487:SF12	3-OXO-DELTA(4,5)-STEROID 5-BETA-REDUCTASE ISOFORM X1	coord: 1..389
None	No IPR available	PANTHER	PTHR32487	3-OXO-DELTA(4,5)-STEROID 5-BETA-REDUCTASE	coord: 1..389
None	No IPR available	CDD	cd08948	5beta-POR_like_SDR_a	coord: 17..328 e-value: 2.51837E-98 score: 293.383
IPR036291	NAD(P)-binding domain superfamily	SUPERFAMILY	51735	NAD(P)-binding Rossmann-fold domains	coord: 17..294

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmoCh01G006910.1	CmoCh01G006910.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0003824	catalytic activity