CmoCh01G005120 (gene) Cucurbita moschata (Rifu) v1

Overview


Name	CmoCh01G005120
Type	gene
Organism	Cucurbita moschata (Cucurbita moschata (Rifu) v1)
Description	E3 ubiquitin-protein ligase RNF181-like
Location	Cmo_Chr01: 2463141 .. 2464896 (+)
RNA-Seq Expression	CmoCh01G005120
Synteny	CmoCh01G005120

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAAAAACCTTTCTTCATCTACTTGCTCTTGTTGTCGTCGTGGTCGTCATTTGTATGTGCCAACATTTTCCCTCAGCTTTTTGTCATCTTCCAAAGCTAGGTACAGTCACTAACAAACTTATATTATTTTCGTCGTCTGTTTCGGGACCAGTCAATTCTATGTTCCAATTTGATCACACTGCTTGATTCTGCTTCTTGGAAGCTAAACATAAAACTCTTGCCTTGTATTTTGTACATTCTTTTTTATATAGAGAAGTTATCTACAGGGAGATTTTATATTGTATACAATTTGCTGGGCATGTCAAATAAAAAATAGTAGATAGCATGAGAAAGATCATAAACTTACCTGTTATAAACTAAAGTGGCACATCTTTATTTGAGAGCTGAAGCCAGAATGTTTGTTTATTTAGCATCTAAATGGGGTTGATCTTGAGTTGAGAGCAAGTATTCAAACAGTTCTGCCCGCGTTTGTGCTACAACAACGTAGAATTGTTCGAGTCAAACCAATTTTAAACTCTATGCCAATTTTAAACTCTATGCAAGATCAAATCCCTAATACCGGAACAATAAGTTGCAACAAGATGATGAACAATGTTAAGATGCAATAGGACTACTCTAAAACGACTCAAACTCTTCAAATACCTTCAATTCAACAAGTTTTGATCTATGATATAGTTGTTTGGTCTAGTGTGCATATAAAAGAAGTCGACATTAGAGTACATAGGACGCGGAGATACCATGTTATCCCATTTGGATCGTCACAATGATCTAATCACTATTCCATTAGATTAAGTTAAAAGTACGAAATTACTTCGAATCATTTCTAAATTTAGAGATTAAATTGTATTTTTTATAAGTTCATAAACCAAATCACGATTTATCACGATCATTTTTGAATGGGTAAGGATCGAGACGAAGTGTAATGTCTTTTTTAACCCACATCAAAATCTCATCCTATTATTAATATCATTTCATGGCAGTGATATAGAATGAAAAGGACAGGCACCGGCTTTGCCTTGCATTGGGGGCACTGATGGATGAAGAGGCAATTTATGTATTCATTATCAAACAAACTCCAAACGCCAATAATTCATTACCCTTCCCTTCTTCAATCTCCAAACCCAGAAGGAACCTCCGATTCCCACATCCATGGCCTCCGATTCCAACTCCCCTGAAATCTCCTCTGTTTTCGACACCCTCATCGGAAACAGAGACCTCTCTCTCTTCATGCCCGTCCTCTTCGGCTTCTCCGCCGCCGACCCACTTCAACAATCCCAAAACCCCGCCGACCCAGATCGCCAAAACGCCAATCGGAGCGACAGGATATTTCTGGTCAACCCTTTTACTCAGAGCATGGTCGTGATCGAGGGGCGTTCGAGCTTGGAAGCACTGATGCGTGATCTCGGCGGGAAGGACGGCCAGCCGCCGGCGTCCAGAGCATCCATAGAAGCGCTCCCCACCGTTCAAATCATGGCAGAGGAGTCGGGTTGTGAGTGTGTGATCTGCTTGGATGAGTGGAAGATTGGGGCGGTGGCTAAAGAACTGCCCTGCGGACACAAATTTCATCCCGACTGTATAGAGAAATGGCTTGGGATTCATGGGAATTGCCCTCTTTGTAGGTGCAAGCTGCCGGATGATGTTGATCGGAAGAACAACGAGGGGCGGCGGGCGAGGGAGATTTGGGTCGGTTTTTCTTTCAATAATGATCGGAGAAGTGAGGGTTCTAATGGAGTTTCTTCAGATCATGGAGCTTAG

mRNA sequence

ATGAAAAACCTTTCTTCATCTACTTGCTCTTGTTGTCGTCGTGGTCGTCATTTGTATGTGCCAACATTTTCCCTCAGCTTTTTGTCATCTTCCAAAGCTAGGCACCGGCTTTGCCTTGCATTGGGGGCACTGATGGATGAAGAGGCAATTTATAAGGAACCTCCGATTCCCACATCCATGGCCTCCGATTCCAACTCCCCTGAAATCTCCTCTGTTTTCGACACCCTCATCGGAAACAGAGACCTCTCTCTCTTCATGCCCGTCCTCTTCGGCTTCTCCGCCGCCGACCCACTTCAACAATCCCAAAACCCCGCCGACCCAGATCGCCAAAACGCCAATCGGAGCGACAGGATATTTCTGGTCAACCCTTTTACTCAGAGCATGGTCGTGATCGAGGGGCGTTCGAGCTTGGAAGCACTGATGCGTGATCTCGGCGGGAAGGACGGCCAGCCGCCGGCGTCCAGAGCATCCATAGAAGCGCTCCCCACCGTTCAAATCATGGCAGAGGAGTCGGGTTGTGAGTGTGTGATCTGCTTGGATGAGTGGAAGATTGGGGCGGTGGCTAAAGAACTGCCCTGCGGACACAAATTTCATCCCGACTGTATAGAGAAATGGCTTGGGATTCATGGGAATTGCCCTCTTTGTAGGTGCAAGCTGCCGGATGATGTTGATCGGAAGAACAACGAGGGGCGGCGGGCGAGGGAGATTTGGGTCGGTTTTTCTTTCAATAATGATCGGAGAAGTGAGGGTTCTAATGGAGTTTCTTCAGATCATGGAGCTTAG

Coding sequence (CDS)

ATGAAAAACCTTTCTTCATCTACTTGCTCTTGTTGTCGTCGTGGTCGTCATTTGTATGTGCCAACATTTTCCCTCAGCTTTTTGTCATCTTCCAAAGCTAGGCACCGGCTTTGCCTTGCATTGGGGGCACTGATGGATGAAGAGGCAATTTATAAGGAACCTCCGATTCCCACATCCATGGCCTCCGATTCCAACTCCCCTGAAATCTCCTCTGTTTTCGACACCCTCATCGGAAACAGAGACCTCTCTCTCTTCATGCCCGTCCTCTTCGGCTTCTCCGCCGCCGACCCACTTCAACAATCCCAAAACCCCGCCGACCCAGATCGCCAAAACGCCAATCGGAGCGACAGGATATTTCTGGTCAACCCTTTTACTCAGAGCATGGTCGTGATCGAGGGGCGTTCGAGCTTGGAAGCACTGATGCGTGATCTCGGCGGGAAGGACGGCCAGCCGCCGGCGTCCAGAGCATCCATAGAAGCGCTCCCCACCGTTCAAATCATGGCAGAGGAGTCGGGTTGTGAGTGTGTGATCTGCTTGGATGAGTGGAAGATTGGGGCGGTGGCTAAAGAACTGCCCTGCGGACACAAATTTCATCCCGACTGTATAGAGAAATGGCTTGGGATTCATGGGAATTGCCCTCTTTGTAGGTGCAAGCTGCCGGATGATGTTGATCGGAAGAACAACGAGGGGCGGCGGGCGAGGGAGATTTGGGTCGGTTTTTCTTTCAATAATGATCGGAGAAGTGAGGGTTCTAATGGAGTTTCTTCAGATCATGGAGCTTAG

Protein sequence

MKNLSSSTCSCCRRGRHLYVPTFSLSFLSSSKARHRLCLALGALMDEEAIYKEPPIPTSMASDSNSPEISSVFDTLIGNRDLSLFMPVLFGFSAADPLQQSQNPADPDRQNANRSDRIFLVNPFTQSMVVIEGRSSLEALMRDLGGKDGQPPASRASIEALPTVQIMAEESGCECVICLDEWKIGAVAKELPCGHKFHPDCIEKWLGIHGNCPLCRCKLPDDVDRKNNEGRRAREIWVGFSFNNDRRSEGSNGVSSDHGA

Homology

[Close]

BLAST of CmoCh01G005120 vs. ExPASy Swiss-Prot
Match: Q9LQX2 (E3 ubiquitin-protein ligase MPSR1 OS=Arabidopsis thaliana OX=3702 GN=MPSR1 PE=1 SV=1)

HSP 1 Score: 180.3 bits (456), Expect = 2.9e-44
Identity = 100/206 (48.54%), Postives = 131/206 (63.59%), Query Frame = 0


Query: 68  EISSVFDTLIGNRDLSLFMPVLFGFSAADPLQQSQNPADPDRQNANRSDRIFLVNPFTQS 127
           E SSV    + NRDL LF+P L GFS     Q+S N  D D   A+  +RI LVNPFTQ 
Sbjct: 12  ETSSVSGRFLRNRDLYLFLPFLLGFSD----QESSNGDDDD--VASSRERIILVNPFTQG 71

Query: 128 MVVIEGRSSLEALMRDL--GGKDGQPPASRASIEALPTVQIMAEESGC--ECVICLDEWK 187
           M+V+EG S +  L+R L    ++G+PPAS+ASI+A+P V+I     GC  ECVICL+EWK
Sbjct: 72  MIVLEGSSGMNPLLRSLLESREEGRPPASKASIDAMPIVEI----DGCEGECVICLEEWK 131

Query: 188 IGAVAKELPCGHKFHPDCIEKWLGIHGNCPLCRCKLP---DDVDRKNNEGRRAREIWVGF 247
                KE+PC H+FH  CIEKWLG HG+CP+CR ++P   D++ +K N+G    EIWV F
Sbjct: 132 SEETVKEMPCKHRFHGGCIEKWLGFHGSCPVCRYEMPVDGDEIGKKRNDG---NEIWVRF 191

Query: 248 SFNNDRR--------SEGSNGVSSDH 259
           SFN+ RR           S+GV S++
Sbjct: 192 SFNDGRRIRDFSAQDGGNSDGVESEN 204

[Close]

BLAST of CmoCh01G005120 vs. ExPASy Swiss-Prot
Match: P0CH30 (E3 ubiquitin-protein ligase RING1 OS=Gossypium hirsutum OX=3635 GN=RING1 PE=1 SV=1)

HSP 1 Score: 95.5 bits (236), Expect = 9.4e-19
Identity = 68/203 (33.50%), Postives = 101/203 (49.75%), Query Frame = 0


Query: 58  TSMASDSNSPEISSVFDTLIGNRDLSLFMPVLFGFSAADPLQQSQNPADPDRQNANRSDR 117
           +S + D N+P +S    T  G  D   F P  F  +  + L+ S    +   QN N SD+
Sbjct: 106 SSASIDPNNPSLSG--PTRSGRGDPFAFDPFTFIQNHLNDLRSSGAQIEFVIQN-NPSDQ 165

Query: 118 IFLVNPFTQSMVVIEGRSSLEALMRDLGGKD----GQPPASRASIEALPTVQIMAEESGC 177
            F +        +  G   LE L++ L   D    G PPAS+++IEALP V I       
Sbjct: 166 GFRLPANIGDYFIGPG---LEQLIQQLAENDPNRYGTPPASKSAIEALPLVNITKSNLNS 225

Query: 178 E---CVICLDEWKIGAVAKELPCGHKFHPDCIEKWLGIHGNCPLCRCKLPDDVDRKNNEG 237
           E   C +C+D+++ G  AK++PC H +H DC+  WL +H +CP+CR +LP D        
Sbjct: 226 EFNQCAVCMDDFEEGTEAKQMPCKHLYHKDCLLPWLELHNSCPVCRHELPTDDPDYERRV 285

Query: 238 RRAREIWVGFSFNND-RRSEGSN 253
           R A+    G   +N  +RS+G N
Sbjct: 286 RGAQGTSGGNDGDNSGQRSDGDN 302

[Close]

BLAST of CmoCh01G005120 vs. ExPASy Swiss-Prot
Match: Q6AVN2 (E3 ubiquitin-protein ligase SIRP1 OS=Oryza sativa subsp. japonica OX=39947 GN=SIRP1 PE=1 SV=1)

HSP 1 Score: 95.1 bits (235), Expect = 1.2e-18
Identity = 40/93 (43.01%), Postives = 60/93 (64.52%), Query Frame = 0


Query: 137 LEALMRDLGGKDGQPPASRASIEALPTVQIMAEESGCECVICLDEWKIGAVAKELPCGHK 196
           L+ L      ++G PPA + ++EALPTV+I   E    C +CLD+ ++G+ AK++PC HK
Sbjct: 164 LQHLAESDPSRNGTPPAKKEAVEALPTVKI---EEVVSCSVCLDDLEVGSQAKQMPCEHK 223

Query: 197 FHPDCIEKWLGIHGNCPLCRCKLPDDVDRKNNE 230
           FH  CI  WL +H +CP+CR +LP +  +  NE
Sbjct: 224 FHSSCILPWLELHSSCPVCRFELPSEETKDLNE 253

[Close]

BLAST of CmoCh01G005120 vs. ExPASy Swiss-Prot
Match: Q8LPN7 (E3 ubiquitin-protein ligase RING1-like OS=Arabidopsis thaliana OX=3702 GN=At3g19950 PE=1 SV=1)

HSP 1 Score: 92.8 bits (229), Expect = 6.1e-18
Identity = 49/132 (37.12%), Postives = 74/132 (56.06%), Query Frame = 0


Query: 137 LEALMRDLGGKD----GQPPASRASIEALPTVQI---MAEESGCECVICLDEWKIGAVAK 196
           LE L++ L   D    G PPAS+++I+ALPTV++   M +    +C +C+DE++ G+  K
Sbjct: 171 LEQLIQQLAENDPNRYGTPPASKSAIDALPTVKVTKDMLKSEMNQCAVCMDEFEDGSDVK 230

Query: 197 ELPCGHKFHPDCIEKWLGIHGNCPLCRCKLP-DDVDRKNNEGRRAREIWVGFSFNNDRRS 256
           ++PC H FH DC+  WL +H +CP+CR +LP DD D +N                   RS
Sbjct: 231 QMPCKHVFHQDCLLPWLELHNSCPVCRFELPTDDPDYEN-------------------RS 283

Query: 257 EGSNGVSSDHGA 261
           +GS G     G+
Sbjct: 291 QGSQGSGDGQGS 283

[Close]

BLAST of CmoCh01G005120 vs. ExPASy Swiss-Prot
Match: Q9SNB6 (E3 ubiquitin-protein ligase RDUF1 OS=Arabidopsis thaliana OX=3702 GN=RDUF1 PE=1 SV=1)

HSP 1 Score: 91.3 bits (225), Expect = 1.8e-17
Identity = 42/106 (39.62%), Postives = 65/106 (61.32%), Query Frame = 0


Query: 130 VIEGRSSLEALMRDLGGKDGQPPASRASIEALPTVQIMAEESGCE--CVICLDEWKIGAV 189
           ++E  S +EA    + G+ G PPAS+++IE+LP V+I    +  E  C +C + ++ G  
Sbjct: 169 LLEQLSQIEASGNGI-GRSGNPPASKSAIESLPRVEISDCHTKAEANCAVCTEVFEAGIE 228

Query: 190 AKELPCGHKFHPDCIEKWLGIHGNCPLCRCKLPDDVDRKNNEGRRA 234
            +E+PC H FH DCI  WL I  +CP+CR +LP D  +++NE   A
Sbjct: 229 GREMPCKHIFHGDCIVPWLSIRNSCPVCRFELPSDPIQRSNEEEHA 273

[Close]

BLAST of CmoCh01G005120 vs. ExPASy TrEMBL
Match: A0A6J1K8F1 (E3 ubiquitin-protein ligase RNF181-like OS=Cucurbita maxima OX=3661 GN=LOC111492631 PE=4 SV=1)

HSP 1 Score: 423.3 bits (1087), Expect = 7.2e-115
Identity = 201/209 (96.17%), Postives = 205/209 (98.09%), Query Frame = 0


Query: 52  KEPPIPTSMASDSNSPEISSVFDTLIGNRDLSLFMPVLFGFSAADPLQQSQNPADPDRQN 111
           ++PPIPTSMASDSNSPEISSVFDT IGNRDLSLFMP LFGFS ADPLQQSQNPADPDRQN
Sbjct: 30  EQPPIPTSMASDSNSPEISSVFDTFIGNRDLSLFMPFLFGFSTADPLQQSQNPADPDRQN 89

Query: 112 ANRSDRIFLVNPFTQSMVVIEGRSSLEALMRDLGGKDGQPPASRASIEALPTVQIMAEES 171
           ANRSDRIFLVNPFTQSMVVIEGRSSLEALMRDLGGKDGQPPASRASIEALPTVQIMAEE 
Sbjct: 90  ANRSDRIFLVNPFTQSMVVIEGRSSLEALMRDLGGKDGQPPASRASIEALPTVQIMAEEL 149

Query: 172 GCECVICLDEWKIGAVAKELPCGHKFHPDCIEKWLGIHGNCPLCRCKLPDDVDRKNNEGR 231
           GCECVICLDEW+IGAVAKELPCGHKFHPDCIEKWLG+HGNCPLCRCKLPDDVDRKNNEGR
Sbjct: 150 GCECVICLDEWEIGAVAKELPCGHKFHPDCIEKWLGVHGNCPLCRCKLPDDVDRKNNEGR 209

Query: 232 RAREIWVGFSFNNDRRSEGSNGVSSDHGA 261
           RAREIWVGFSFNNDRRSEGSNGVSSDHGA
Sbjct: 210 RAREIWVGFSFNNDRRSEGSNGVSSDHGA 238

[Close]

BLAST of CmoCh01G005120 vs. ExPASy TrEMBL
Match: A0A6J1GCB1 (E3 ubiquitin-protein ligase RNF181-like OS=Cucurbita moschata OX=3662 GN=LOC111452865 PE=4 SV=1)

HSP 1 Score: 418.7 bits (1075), Expect = 1.8e-113
Identity = 201/201 (100.00%), Postives = 201/201 (100.00%), Query Frame = 0


Query: 60  MASDSNSPEISSVFDTLIGNRDLSLFMPVLFGFSAADPLQQSQNPADPDRQNANRSDRIF 119
           MASDSNSPEISSVFDTLIGNRDLSLFMPVLFGFSAADPLQQSQNPADPDRQNANRSDRIF
Sbjct: 1   MASDSNSPEISSVFDTLIGNRDLSLFMPVLFGFSAADPLQQSQNPADPDRQNANRSDRIF 60

Query: 120 LVNPFTQSMVVIEGRSSLEALMRDLGGKDGQPPASRASIEALPTVQIMAEESGCECVICL 179
           LVNPFTQSMVVIEGRSSLEALMRDLGGKDGQPPASRASIEALPTVQIMAEESGCECVICL
Sbjct: 61  LVNPFTQSMVVIEGRSSLEALMRDLGGKDGQPPASRASIEALPTVQIMAEESGCECVICL 120

Query: 180 DEWKIGAVAKELPCGHKFHPDCIEKWLGIHGNCPLCRCKLPDDVDRKNNEGRRAREIWVG 239
           DEWKIGAVAKELPCGHKFHPDCIEKWLGIHGNCPLCRCKLPDDVDRKNNEGRRAREIWVG
Sbjct: 121 DEWKIGAVAKELPCGHKFHPDCIEKWLGIHGNCPLCRCKLPDDVDRKNNEGRRAREIWVG 180

Query: 240 FSFNNDRRSEGSNGVSSDHGA 261
           FSFNNDRRSEGSNGVSSDHGA
Sbjct: 181 FSFNNDRRSEGSNGVSSDHGA 201

[Close]

BLAST of CmoCh01G005120 vs. ExPASy TrEMBL
Match: A0A6P4B064 (E3 ubiquitin-protein ligase MPSR1-like OS=Ziziphus jujuba OX=326968 GN=LOC107429014 PE=4 SV=1)

HSP 1 Score: 246.5 bits (628), Expect = 1.2e-61
Identity = 121/206 (58.74%), Postives = 159/206 (77.18%), Query Frame = 0


Query: 60  MASDSNSPEISSVFDTLIGNRDLSLFMPVLFGFSAADPLQQSQNPADPDRQNANRS---D 119
           MAS++++ E+S++F+ L+ NRDLSLF+P +FGF+ + P     NP DPD +N NR+   D
Sbjct: 1   MASEADASELSTMFERLVRNRDLSLFLPFIFGFANSLP---RANPQDPDEENENRATSRD 60

Query: 120 RIFLVNPFTQSMVVIEGRSSLEALMRDLGGKDGQPPASRASIEALPTVQIMAEESGCECV 179
           R+ L+NPFTQ MVVI+G SSL++++RDLGGKDGQPPAS+ASIEA+P V+   EE G ECV
Sbjct: 61  RVILINPFTQGMVVIDGFSSLDSVLRDLGGKDGQPPASKASIEAMPGVE--TEEEGLECV 120

Query: 180 ICLDEWKIGAVAKELPCGHKFHPDCIEKWLGIHGNCPLCRCKLPDDVD----RKNNEGRR 239
           ICL+E+++G VAKE+PC HKFH DCIEKWLGIHG+CP+CR  +P D +    ++  EGR 
Sbjct: 121 ICLEEFEVGGVAKEMPCKHKFHGDCIEKWLGIHGSCPVCRHNMPVDEEEMGKKREEEGRE 180

Query: 240 AR--EIWVGFSFNNDRRSEGSNGVSS 257
            R  E+WV FSFN+ RRSE SN VSS
Sbjct: 181 RRRGEVWVSFSFNSGRRSEDSNQVSS 201

[Close]

BLAST of CmoCh01G005120 vs. ExPASy TrEMBL
Match: A0A6J1DR69 (E3 ubiquitin-protein ligase RNF181 OS=Momordica charantia OX=3673 GN=LOC111023495 PE=4 SV=1)

HSP 1 Score: 237.7 bits (605), Expect = 5.6e-59
Identity = 129/211 (61.14%), Postives = 152/211 (72.04%), Query Frame = 0


Query: 61  ASDSNSPEISSVFDTLIGNRDLSLFMPVLFGFS---AADPLQQSQNPADPDRQNANRSDR 120
           +S+  SPE SS+F           F P LFGF+     DP Q+S+NPA+        S+R
Sbjct: 3   SSEPESPETSSLF----------AFTPFLFGFTNNRRDDPNQESRNPAN------RESER 62

Query: 121 IFLVNPFTQSMVVIEGRSSLEALMRDLGGKDGQPPASRASIEALPTVQIMAEESGCECVI 180
            FLVNPFTQSMVVIEG S+LE+L+RDLG KDG PPAS+ASIEALP VQ++ E    ECVI
Sbjct: 63  FFLVNPFTQSMVVIEGSSALESLLRDLGSKDGPPPASKASIEALPAVQLLEEG---ECVI 122

Query: 181 CLDEWKIGAVAKELPCGHKFHPDCIEKWLGIHGNCPLCRCKLP-DDVDRKNNE----GRR 240
           CLDEW+IGA AKELPC HKFHP+CIEKWLGIHG+CP+CR K+P DD D K N+    GRR
Sbjct: 123 CLDEWEIGATAKELPCKHKFHPNCIEKWLGIHGSCPICRHKMPVDDGDGKKNDVVGRGRR 182

Query: 241 AREIWVGFSFNNDRRSEGSNGV---SSDHGA 261
            REIWVGFSFNND RS+ SN +   +SDH A
Sbjct: 183 LREIWVGFSFNNDIRSDDSNPLPSSASDHEA 194

[Close]

BLAST of CmoCh01G005120 vs. ExPASy TrEMBL
Match: A0A6P5Y3C4 (E3 ubiquitin-protein ligase RING1-like OS=Durio zibethinus OX=66656 GN=LOC111288343 PE=4 SV=1)

HSP 1 Score: 228.0 bits (580), Expect = 4.5e-56
Identity = 113/208 (54.33%), Postives = 152/208 (73.08%), Query Frame = 0


Query: 60  MASDSNSPEISSVFDTLIGNRDLSLFMPVLFGFSAADPLQQS---QNPADPDRQNANRS- 119
           MAS++ +PEISS+F+  +  RDL LF+P + GFS  +    +   +NP +PD++ +  + 
Sbjct: 1   MASETEAPEISSLFERFLRQRDLHLFLPFILGFSNNNNSNNNISRENPEEPDQETSQETT 60

Query: 120 ---DRIFLVNPFTQSMVVIEGRSSLEALMRDLGGKDGQPPASRASIEALPTVQIMAEESG 179
              ++I L+NPFTQ MVVIEG S+LEAL+  L  KDGQPPAS+ASIEA+P+V+I   E G
Sbjct: 61  SLREKIILINPFTQGMVVIEGASNLEALLLGLANKDGQPPASKASIEAMPSVEIGESEDG 120

Query: 180 CECVICLDEWKIGAVAKELPCGHKFHPDCIEKWLGIHGNCPLCRCKLP--DDVDRKNNEG 239
            ECV+CL+EWK G VAKE+PC HKFH +CI KWLG+HG+CP+CR K+P  +++ +K +E 
Sbjct: 121 -ECVVCLEEWKPGEVAKEMPCKHKFHDECIRKWLGLHGSCPVCRYKMPVEEEIGKKRDEE 180

Query: 240 RR--AREIWVGFSFNNDRRSEGSNGVSS 257
           RR  AREIWV FSFN+ RRS  SN V S
Sbjct: 181 RRRSAREIWVSFSFNSGRRSGDSNQVPS 207

[Close]

BLAST of CmoCh01G005120 vs. NCBI nr
Match: XP_022997776.1 (E3 ubiquitin-protein ligase RNF181-like [Cucurbita maxima])

HSP 1 Score: 423.3 bits (1087), Expect = 1.5e-114
Identity = 201/209 (96.17%), Postives = 205/209 (98.09%), Query Frame = 0


Query: 52  KEPPIPTSMASDSNSPEISSVFDTLIGNRDLSLFMPVLFGFSAADPLQQSQNPADPDRQN 111
           ++PPIPTSMASDSNSPEISSVFDT IGNRDLSLFMP LFGFS ADPLQQSQNPADPDRQN
Sbjct: 30  EQPPIPTSMASDSNSPEISSVFDTFIGNRDLSLFMPFLFGFSTADPLQQSQNPADPDRQN 89

Query: 112 ANRSDRIFLVNPFTQSMVVIEGRSSLEALMRDLGGKDGQPPASRASIEALPTVQIMAEES 171
           ANRSDRIFLVNPFTQSMVVIEGRSSLEALMRDLGGKDGQPPASRASIEALPTVQIMAEE 
Sbjct: 90  ANRSDRIFLVNPFTQSMVVIEGRSSLEALMRDLGGKDGQPPASRASIEALPTVQIMAEEL 149

Query: 172 GCECVICLDEWKIGAVAKELPCGHKFHPDCIEKWLGIHGNCPLCRCKLPDDVDRKNNEGR 231
           GCECVICLDEW+IGAVAKELPCGHKFHPDCIEKWLG+HGNCPLCRCKLPDDVDRKNNEGR
Sbjct: 150 GCECVICLDEWEIGAVAKELPCGHKFHPDCIEKWLGVHGNCPLCRCKLPDDVDRKNNEGR 209

Query: 232 RAREIWVGFSFNNDRRSEGSNGVSSDHGA 261
           RAREIWVGFSFNNDRRSEGSNGVSSDHGA
Sbjct: 210 RAREIWVGFSFNNDRRSEGSNGVSSDHGA 238

[Close]

BLAST of CmoCh01G005120 vs. NCBI nr
Match: XP_023523370.1 (E3 ubiquitin-protein ligase RNF181-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 419.9 bits (1078), Expect = 1.7e-113
Identity = 201/209 (96.17%), Postives = 205/209 (98.09%), Query Frame = 0


Query: 52  KEPPIPTSMASDSNSPEISSVFDTLIGNRDLSLFMPVLFGFSAADPLQQSQNPADPDRQN 111
           ++PPI TSMASDSNSPEISSVFDTLIGNRDLSLFMP LFGFSAADPLQQSQNPADPDRQN
Sbjct: 30  EQPPITTSMASDSNSPEISSVFDTLIGNRDLSLFMPFLFGFSAADPLQQSQNPADPDRQN 89

Query: 112 ANRSDRIFLVNPFTQSMVVIEGRSSLEALMRDLGGKDGQPPASRASIEALPTVQIMAEES 171
           ANRSDRIFLVNPFTQSMVVIEGRSSLEALMRDLGGKDGQPPASRASIEALPTVQIMAEE 
Sbjct: 90  ANRSDRIFLVNPFTQSMVVIEGRSSLEALMRDLGGKDGQPPASRASIEALPTVQIMAEEL 149

Query: 172 GCECVICLDEWKIGAVAKELPCGHKFHPDCIEKWLGIHGNCPLCRCKLPDDVDRKNNEGR 231
           GCECVICLDEW+IGAVAKELPCGHKFHPDCIEKWLG+HGNCPLCRCKLPDDVDRKNNEGR
Sbjct: 150 GCECVICLDEWEIGAVAKELPCGHKFHPDCIEKWLGVHGNCPLCRCKLPDDVDRKNNEGR 209

Query: 232 RAREIWVGFSFNNDRRSEGSNGVSSDHGA 261
           RAREIWVGFSFNNDRRSEGSNGVSSD GA
Sbjct: 210 RAREIWVGFSFNNDRRSEGSNGVSSDDGA 238

[Close]

BLAST of CmoCh01G005120 vs. NCBI nr
Match: XP_022949551.1 (E3 ubiquitin-protein ligase RNF181-like [Cucurbita moschata])

HSP 1 Score: 418.7 bits (1075), Expect = 3.7e-113
Identity = 201/201 (100.00%), Postives = 201/201 (100.00%), Query Frame = 0


Query: 60  MASDSNSPEISSVFDTLIGNRDLSLFMPVLFGFSAADPLQQSQNPADPDRQNANRSDRIF 119
           MASDSNSPEISSVFDTLIGNRDLSLFMPVLFGFSAADPLQQSQNPADPDRQNANRSDRIF
Sbjct: 1   MASDSNSPEISSVFDTLIGNRDLSLFMPVLFGFSAADPLQQSQNPADPDRQNANRSDRIF 60

Query: 120 LVNPFTQSMVVIEGRSSLEALMRDLGGKDGQPPASRASIEALPTVQIMAEESGCECVICL 179
           LVNPFTQSMVVIEGRSSLEALMRDLGGKDGQPPASRASIEALPTVQIMAEESGCECVICL
Sbjct: 61  LVNPFTQSMVVIEGRSSLEALMRDLGGKDGQPPASRASIEALPTVQIMAEESGCECVICL 120

Query: 180 DEWKIGAVAKELPCGHKFHPDCIEKWLGIHGNCPLCRCKLPDDVDRKNNEGRRAREIWVG 239
           DEWKIGAVAKELPCGHKFHPDCIEKWLGIHGNCPLCRCKLPDDVDRKNNEGRRAREIWVG
Sbjct: 121 DEWKIGAVAKELPCGHKFHPDCIEKWLGIHGNCPLCRCKLPDDVDRKNNEGRRAREIWVG 180

Query: 240 FSFNNDRRSEGSNGVSSDHGA 261
           FSFNNDRRSEGSNGVSSDHGA
Sbjct: 181 FSFNNDRRSEGSNGVSSDHGA 201

[Close]

BLAST of CmoCh01G005120 vs. NCBI nr
Match: KAG6607122.1 (E3 ubiquitin-protein ligase MPSR1, partial [Cucurbita argyrosperma subsp. sororia] >KAG7036814.1 E3 ubiquitin-protein ligase RING1-like protein, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 411.0 bits (1055), Expect = 7.7e-111
Identity = 197/201 (98.01%), Postives = 198/201 (98.51%), Query Frame = 0


Query: 60  MASDSNSPEISSVFDTLIGNRDLSLFMPVLFGFSAADPLQQSQNPADPDRQNANRSDRIF 119
           MASDSNSPE SSVFDTLIGNRDLSLFMP LFGFSAADPLQQSQNPADPDRQNANRSDRIF
Sbjct: 1   MASDSNSPETSSVFDTLIGNRDLSLFMPFLFGFSAADPLQQSQNPADPDRQNANRSDRIF 60

Query: 120 LVNPFTQSMVVIEGRSSLEALMRDLGGKDGQPPASRASIEALPTVQIMAEESGCECVICL 179
           LVNPFTQSMVVIEGRSSLEALMRDLGGKDGQPPASRASIEALPTVQIMAEESGCECVICL
Sbjct: 61  LVNPFTQSMVVIEGRSSLEALMRDLGGKDGQPPASRASIEALPTVQIMAEESGCECVICL 120

Query: 180 DEWKIGAVAKELPCGHKFHPDCIEKWLGIHGNCPLCRCKLPDDVDRKNNEGRRAREIWVG 239
           DEW+IGAVAKELPCGHKFHPDCIEKWLGIHGNCPLCRCKLPDDVDRKNNEGRRAREIWVG
Sbjct: 121 DEWEIGAVAKELPCGHKFHPDCIEKWLGIHGNCPLCRCKLPDDVDRKNNEGRRAREIWVG 180

Query: 240 FSFNNDRRSEGSNGVSSDHGA 261
           FSF NDRRSEGSNGVSSDHGA
Sbjct: 181 FSFTNDRRSEGSNGVSSDHGA 201

[Close]

BLAST of CmoCh01G005120 vs. NCBI nr
Match: XP_015895111.1 (E3 ubiquitin-protein ligase MPSR1-like [Ziziphus jujuba])

HSP 1 Score: 246.5 bits (628), Expect = 2.5e-61
Identity = 121/206 (58.74%), Postives = 159/206 (77.18%), Query Frame = 0


Query: 60  MASDSNSPEISSVFDTLIGNRDLSLFMPVLFGFSAADPLQQSQNPADPDRQNANRS---D 119
           MAS++++ E+S++F+ L+ NRDLSLF+P +FGF+ + P     NP DPD +N NR+   D
Sbjct: 1   MASEADASELSTMFERLVRNRDLSLFLPFIFGFANSLP---RANPQDPDEENENRATSRD 60

Query: 120 RIFLVNPFTQSMVVIEGRSSLEALMRDLGGKDGQPPASRASIEALPTVQIMAEESGCECV 179
           R+ L+NPFTQ MVVI+G SSL++++RDLGGKDGQPPAS+ASIEA+P V+   EE G ECV
Sbjct: 61  RVILINPFTQGMVVIDGFSSLDSVLRDLGGKDGQPPASKASIEAMPGVE--TEEEGLECV 120

Query: 180 ICLDEWKIGAVAKELPCGHKFHPDCIEKWLGIHGNCPLCRCKLPDDVD----RKNNEGRR 239
           ICL+E+++G VAKE+PC HKFH DCIEKWLGIHG+CP+CR  +P D +    ++  EGR 
Sbjct: 121 ICLEEFEVGGVAKEMPCKHKFHGDCIEKWLGIHGSCPVCRHNMPVDEEEMGKKREEEGRE 180

Query: 240 AR--EIWVGFSFNNDRRSEGSNGVSS 257
            R  E+WV FSFN+ RRSE SN VSS
Sbjct: 181 RRRGEVWVSFSFNSGRRSEDSNQVSS 201

[Close]

BLAST of CmoCh01G005120 vs. TAIR 10
Match: AT1G26800.1 (RING/U-box superfamily protein )

HSP 1 Score: 180.3 bits (456), Expect = 2.1e-45
Identity = 100/206 (48.54%), Postives = 131/206 (63.59%), Query Frame = 0


Query: 68  EISSVFDTLIGNRDLSLFMPVLFGFSAADPLQQSQNPADPDRQNANRSDRIFLVNPFTQS 127
           E SSV    + NRDL LF+P L GFS     Q+S N  D D   A+  +RI LVNPFTQ 
Sbjct: 12  ETSSVSGRFLRNRDLYLFLPFLLGFSD----QESSNGDDDD--VASSRERIILVNPFTQG 71

Query: 128 MVVIEGRSSLEALMRDL--GGKDGQPPASRASIEALPTVQIMAEESGC--ECVICLDEWK 187
           M+V+EG S +  L+R L    ++G+PPAS+ASI+A+P V+I     GC  ECVICL+EWK
Sbjct: 72  MIVLEGSSGMNPLLRSLLESREEGRPPASKASIDAMPIVEI----DGCEGECVICLEEWK 131

Query: 188 IGAVAKELPCGHKFHPDCIEKWLGIHGNCPLCRCKLP---DDVDRKNNEGRRAREIWVGF 247
                KE+PC H+FH  CIEKWLG HG+CP+CR ++P   D++ +K N+G    EIWV F
Sbjct: 132 SEETVKEMPCKHRFHGGCIEKWLGFHGSCPVCRYEMPVDGDEIGKKRNDG---NEIWVRF 191

Query: 248 SFNNDRR--------SEGSNGVSSDH 259
           SFN+ RR           S+GV S++
Sbjct: 192 SFNDGRRIRDFSAQDGGNSDGVESEN 204

[Close]

BLAST of CmoCh01G005120 vs. TAIR 10
Match: AT1G14200.1 (RING/U-box superfamily protein )

HSP 1 Score: 130.2 bits (326), Expect = 2.4e-30
Identity = 77/195 (39.49%), Postives = 109/195 (55.90%), Query Frame = 0


Query: 60  MASDSNSPEISSVFDTLIGNR--DLSLFMPVLFGFSAADPLQQSQNPADPDRQNANRSDR 119
           M+S+++  E SS+F+ +I  R   LS F+PV+   +A +     Q   D   Q   R D 
Sbjct: 1   MSSENDFVEFSSMFERIIQGRGDGLSRFLPVIVALAARED-DDDQGSTD---QTTRRGD- 60

Query: 120 IFLVNPFTQSMVVIEGRSSLEALMRDLGGKDGQPPASRASIEALPTVQI--MAEESGCEC 179
                P +   V+I  RS L+    D GGK G+ PA ++ +E +P V I    E+ G  C
Sbjct: 61  -----PLSPRFVMIGSRSGLDDFFSD-GGKQGRSPALKSEVENMPRVVIGEDKEKYGGSC 120

Query: 180 VICLDEWKIGAVAKELPCGHKFHPDCIEKWLGIHGNCPLCRCKLP-DDVDRKNNEGRRAR 239
            ICLDEW  G VA E+PC HKFH  C+E+WLG H  CP+CR ++P ++V+ +   G    
Sbjct: 121 AICLDEWSKGDVAAEMPCKHKFHSKCVEEWLGRHATCPMCRYEMPVEEVEEEKKIG---- 179

Query: 240 EIWVGFSFN-NDRRS 249
            IW+GFS N  DRR+
Sbjct: 181 -IWIGFSINAGDRRN 179

[Close]

BLAST of CmoCh01G005120 vs. TAIR 10
Match: AT5G56340.1 (RING/U-box superfamily protein )

HSP 1 Score: 107.1 bits (266), Expect = 2.2e-23
Identity = 55/151 (36.42%), Postives = 83/151 (54.97%), Query Frame = 0


Query: 105 ADPDRQNANRSDRIFLVNPFTQSMVVIEGRS----------------SLEALMRDLGGKD 164
           +D +  N+   DR+ ++NP+ QS+VV   ++                 L+ L++ L   D
Sbjct: 172 SDNNWDNSRERDRVIMINPYNQSLVVPSDQNQNHPSLTSLGDYFIGPGLDLLLQHLAEND 231

Query: 165 ----GQPPASRASIEALPTVQIMAEESGCECVICLDEWKIGAVAKELPCGHKFHPDCIEK 224
               G PPA + ++EALPTV+IM      +C +CLD+++ G  AKE+PC HKFH  CI  
Sbjct: 232 PNRQGTPPARKEAVEALPTVKIM---EPLQCSVCLDDFEKGTEAKEMPCKHKFHVRCIVP 291

Query: 225 WLGIHGNCPLCRCKLP---DDVDRKNNEGRR 233
           WL +H +CP+CR +LP   DD D    +  R
Sbjct: 292 WLELHSSCPVCRFELPSSADDDDETKTDSER 319

[Close]

BLAST of CmoCh01G005120 vs. TAIR 10
Match: AT3G13430.1 (RING/U-box superfamily protein )

HSP 1 Score: 97.1 bits (240), Expect = 2.3e-20
Identity = 63/207 (30.43%), Postives = 100/207 (48.31%), Query Frame = 0


Query: 77  IGNRDLSLFMPVLFGFSAADPLQQSQNPADPDRQNANRSDRIFLVNPFTQSMVVIEGR-- 136
           I  R  +  + +L G  A   ++   N  +PD      S+ + L+N F Q + V +    
Sbjct: 112 IRTRHSTAIVNLLQGIRAGLLIESENNEDNPD-----NSELVVLINSFNQRIRVHQDSVD 171

Query: 137 ---------------SSLEALMR-----DLGGKDGQPPASRASIEALPTVQIMAEESGCE 196
                             E L++     DL  + G PPA++ ++EAL  V+I  E+S  +
Sbjct: 172 TTSVPSGSLGDYFIGPGFETLLQRLAENDLNNRYGTPPATKEAVEALAMVKI--EDSLLQ 231

Query: 197 CVICLDEWKIGAVAKELPCGHKFHPDCIEKWLGIHGNCPLCRCKLP-DDVDRKNNEGRRA 256
           C +CLD+++IG  AKE+PC HKFH DC+  WL +H +CP+CR  LP  D D    +   +
Sbjct: 232 CSVCLDDFEIGMEAKEMPCKHKFHSDCLLPWLELHSSCPVCRYLLPTGDDDEPKTDAETS 291

Query: 257 REIWVGFSFNNDRRSEGSNGVSSDHGA 261
           R        N+D   + SN   + +G+
Sbjct: 292 R--------NDDNNEDISNASMASNGS 303

[Close]

BLAST of CmoCh01G005120 vs. TAIR 10
Match: AT3G13430.2 (RING/U-box superfamily protein )

HSP 1 Score: 97.1 bits (240), Expect = 2.3e-20
Identity = 63/207 (30.43%), Postives = 100/207 (48.31%), Query Frame = 0


Query: 77  IGNRDLSLFMPVLFGFSAADPLQQSQNPADPDRQNANRSDRIFLVNPFTQSMVVIEGR-- 136
           I  R  +  + +L G  A   ++   N  +PD      S+ + L+N F Q + V +    
Sbjct: 112 IRTRHSTAIVNLLQGIRAGLLIESENNEDNPD-----NSELVVLINSFNQRIRVHQDSVD 171

Query: 137 ---------------SSLEALMR-----DLGGKDGQPPASRASIEALPTVQIMAEESGCE 196
                             E L++     DL  + G PPA++ ++EAL  V+I  E+S  +
Sbjct: 172 TTSVPSGSLGDYFIGPGFETLLQRLAENDLNNRYGTPPATKEAVEALAMVKI--EDSLLQ 231

Query: 197 CVICLDEWKIGAVAKELPCGHKFHPDCIEKWLGIHGNCPLCRCKLP-DDVDRKNNEGRRA 256
           C +CLD+++IG  AKE+PC HKFH DC+  WL +H +CP+CR  LP  D D    +   +
Sbjct: 232 CSVCLDDFEIGMEAKEMPCKHKFHSDCLLPWLELHSSCPVCRYLLPTGDDDEPKTDAETS 291

Query: 257 REIWVGFSFNNDRRSEGSNGVSSDHGA 261
           R        N+D   + SN   + +G+
Sbjct: 292 R--------NDDNNEDISNASMASNGS 303

The following BLAST results are available for this feature:

BLAST of CmoCh01G005120 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LQX2	2.9e-44	48.54	E3 ubiquitin-protein ligase MPSR1 OS=Arabidopsis thaliana OX=3702 GN=MPSR1 PE=1 ...	[more]
P0CH30	9.4e-19	33.50	E3 ubiquitin-protein ligase RING1 OS=Gossypium hirsutum OX=3635 GN=RING1 PE=1 SV...	[more]
Q6AVN2	1.2e-18	43.01	E3 ubiquitin-protein ligase SIRP1 OS=Oryza sativa subsp. japonica OX=39947 GN=SI...	[more]
Q8LPN7	6.1e-18	37.12	E3 ubiquitin-protein ligase RING1-like OS=Arabidopsis thaliana OX=3702 GN=At3g19...	[more]
Q9SNB6	1.8e-17	39.62	E3 ubiquitin-protein ligase RDUF1 OS=Arabidopsis thaliana OX=3702 GN=RDUF1 PE=1 ...	[more]

BLAST of CmoCh01G005120 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1K8F1	7.2e-115	96.17	E3 ubiquitin-protein ligase RNF181-like OS=Cucurbita maxima OX=3661 GN=LOC111492...	[more]
A0A6J1GCB1	1.8e-113	100.00	E3 ubiquitin-protein ligase RNF181-like OS=Cucurbita moschata OX=3662 GN=LOC1114...	[more]
A0A6P4B064	1.2e-61	58.74	E3 ubiquitin-protein ligase MPSR1-like OS=Ziziphus jujuba OX=326968 GN=LOC107429...	[more]
A0A6J1DR69	5.6e-59	61.14	E3 ubiquitin-protein ligase RNF181 OS=Momordica charantia OX=3673 GN=LOC11102349...	[more]
A0A6P5Y3C4	4.5e-56	54.33	E3 ubiquitin-protein ligase RING1-like OS=Durio zibethinus OX=66656 GN=LOC111288...	[more]

BLAST of CmoCh01G005120 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022997776.1	1.5e-114	96.17	E3 ubiquitin-protein ligase RNF181-like [Cucurbita maxima]	[more]
XP_023523370.1	1.7e-113	96.17	E3 ubiquitin-protein ligase RNF181-like [Cucurbita pepo subsp. pepo]	[more]
XP_022949551.1	3.7e-113	100.00	E3 ubiquitin-protein ligase RNF181-like [Cucurbita moschata]	[more]
KAG6607122.1	7.7e-111	98.01	E3 ubiquitin-protein ligase MPSR1, partial [Cucurbita argyrosperma subsp. sorori...	[more]
XP_015895111.1	2.5e-61	58.74	E3 ubiquitin-protein ligase MPSR1-like [Ziziphus jujuba]	[more]

BLAST of CmoCh01G005120 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G26800.1	2.1e-45	48.54	RING/U-box superfamily protein	[more]
AT1G14200.1	2.4e-30	39.49	RING/U-box superfamily protein	[more]
AT5G56340.1	2.2e-23	36.42	RING/U-box superfamily protein	[more]
AT3G13430.1	2.3e-20	30.43	RING/U-box superfamily protein	[more]
AT3G13430.2	2.3e-20	30.43	RING/U-box superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001841	Zinc finger, RING-type	SMART	SM00184	ring_2	coord: 175..215 e-value: 4.9E-8 score: 42.7
IPR001841	Zinc finger, RING-type	PFAM	PF13639	zf-RING_2	coord: 174..216 e-value: 9.1E-13 score: 48.3
IPR001841	Zinc finger, RING-type	PROSITE	PS50089	ZF_RING_2	coord: 175..216 score: 12.54829
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 142..222 e-value: 3.3E-21 score: 76.7
None	No IPR available	PANTHER	PTHR15710	E3 UBIQUITIN-PROTEIN LIGASE PRAJA	coord: 70..230
None	No IPR available	PANTHER	PTHR15710:SF96	SUBFAMILY NOT NAMED	coord: 70..230
None	No IPR available	CDD	cd16454	RING-H2_PA-TM-RING	coord: 174..216 e-value: 8.96104E-17 score: 70.0233
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 169..222

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmoCh01G005120.1	CmoCh01G005120.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0071218	cellular response to misfolded protein
biological_process	GO:0016567	protein ubiquitination
cellular_component	GO:0005737	cytoplasm
molecular_function	GO:0061630	ubiquitin protein ligase activity