CmoCh01G004720 (gene) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh01G004720
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
Descriptionalpha/beta-Hydrolases superfamily protein
LocationCmo_Chr01: 2255755 .. 2256804 (-)
RNA-Seq ExpressionCmoCh01G004720
SyntenyCmoCh01G004720
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGATATTCAAAAGAATGGCTGCCATGTACATCGATCCAAGGCTCAAACTCCAGATGGCCAAACCCCACCTTCAAAATGGAGGCACGTCAGAAGAGATTGAAGGGCTGATTCGAGTTTATGCAGATGGGCATGTTGAAAGGCCCCCAATCATCCCAAATGTAGCCTGCAGCACCACAGCATCAGACAAAGGCGTAACTGCAAAAGATGTTCTCATAGACATTTTCACAAACACTTGGGCGCGTATTTACATTCCTAACTCTCAACGAAACCTCCCTTTGCTTGTTTACTTCCATGGCGGTGGCTTTTGCGTTGGCTCTGCCGCCTGGAGCTGTTACCATGAATTCCTCCTCAATCTTGCTTCCAAAGCAAAATGTGTAATCCTCTCAATCAACTATCGTCTTTCCCCTGAAAATCGCCTCCCCGCCGCTTATGACGACGGATTCAACGCACTCATGTGGTTGAAACAGCAAGCAATAACCAACCCCATCGAACACAAATGGTGGTTAGGCAATTGCAATCCATCAAAATTGTTCCTCGCCGGCGATAGCGCTGGAGCAAACATATCATACAACACCGCTACAAGGATAGGGCACAACGAATCCACACTCCTAAAACCCCTAACTCTAAAGGGTATAATCCTAATCCAACCTTTCTTCGGCGGAGAATCGCGAACCAAATCAGAAAAGCTCGCCGCTACGCCCTCCTCCACCTCCGCGCTAACTCTCTCCGCCGCCGACACATACTGGCGGCTATCCCTTCCGCCGACGGCCAATCGCGACCACCCATGGAGCAACCCACTGGTGAATGGCGGCGCAAAGCTGAAAGAAATGAAACTTCCACCATTAATGGTATGCATATCGGAGTTGGACATACTGTTGGACAGAAACTTAGAATTCTGTGCAGCCATGGCAGGCGCCGGGAAGAAAGTGGAGAGAGTGATTCTGAAGGGAGTTGGACATGGCTTCCAAATTCTGAACAAAGATCAGATCTCCACTCAGCGAACACAAGAACTGATGGGTTCCATTCGAGCTTTCATTCATCAACAATAG

mRNA sequence

ATGATATTCAAAAGAATGGCTGCCATGTACATCGATCCAAGGCTCAAACTCCAGATGGCCAAACCCCACCTTCAAAATGGAGGCACGTCAGAAGAGATTGAAGGGCTGATTCGAGTTTATGCAGATGGGCATGTTGAAAGGCCCCCAATCATCCCAAATGTAGCCTGCAGCACCACAGCATCAGACAAAGGCGTAACTGCAAAAGATGTTCTCATAGACATTTTCACAAACACTTGGGCGCGTATTTACATTCCTAACTCTCAACGAAACCTCCCTTTGCTTGTTTACTTCCATGGCGGTGGCTTTTGCGTTGGCTCTGCCGCCTGGAGCTGTTACCATGAATTCCTCCTCAATCTTGCTTCCAAAGCAAAATGTGTAATCCTCTCAATCAACTATCGTCTTTCCCCTGAAAATCGCCTCCCCGCCGCTTATGACGACGGATTCAACGCACTCATGTGGTTGAAACAGCAAGCAATAACCAACCCCATCGAACACAAATGGTGGTTAGGCAATTGCAATCCATCAAAATTGTTCCTCGCCGGCGATAGCGCTGGAGCAAACATATCATACAACACCGCTACAAGGATAGGGCACAACGAATCCACACTCCTAAAACCCCTAACTCTAAAGGGTATAATCCTAATCCAACCTTTCTTCGGCGGAGAATCGCGAACCAAATCAGAAAAGCTCGCCGCTACGCCCTCCTCCACCTCCGCGCTAACTCTCTCCGCCGCCGACACATACTGGCGGCTATCCCTTCCGCCGACGGCCAATCGCGACCACCCATGGAGCAACCCACTGGTGAATGGCGGCGCAAAGCTGAAAGAAATGAAACTTCCACCATTAATGGTATGCATATCGGAGTTGGACATACTGTTGGACAGAAACTTAGAATTCTGTGCAGCCATGGCAGGCGCCGGGAAGAAAGTGGAGAGAGTGATTCTGAAGGGAGTTGGACATGGCTTCCAAATTCTGAACAAAGATCAGATCTCCACTCAGCGAACACAAGAACTGATGGGTTCCATTCGAGCTTTCATTCATCAACAATAG

Coding sequence (CDS)

ATGATATTCAAAAGAATGGCTGCCATGTACATCGATCCAAGGCTCAAACTCCAGATGGCCAAACCCCACCTTCAAAATGGAGGCACGTCAGAAGAGATTGAAGGGCTGATTCGAGTTTATGCAGATGGGCATGTTGAAAGGCCCCCAATCATCCCAAATGTAGCCTGCAGCACCACAGCATCAGACAAAGGCGTAACTGCAAAAGATGTTCTCATAGACATTTTCACAAACACTTGGGCGCGTATTTACATTCCTAACTCTCAACGAAACCTCCCTTTGCTTGTTTACTTCCATGGCGGTGGCTTTTGCGTTGGCTCTGCCGCCTGGAGCTGTTACCATGAATTCCTCCTCAATCTTGCTTCCAAAGCAAAATGTGTAATCCTCTCAATCAACTATCGTCTTTCCCCTGAAAATCGCCTCCCCGCCGCTTATGACGACGGATTCAACGCACTCATGTGGTTGAAACAGCAAGCAATAACCAACCCCATCGAACACAAATGGTGGTTAGGCAATTGCAATCCATCAAAATTGTTCCTCGCCGGCGATAGCGCTGGAGCAAACATATCATACAACACCGCTACAAGGATAGGGCACAACGAATCCACACTCCTAAAACCCCTAACTCTAAAGGGTATAATCCTAATCCAACCTTTCTTCGGCGGAGAATCGCGAACCAAATCAGAAAAGCTCGCCGCTACGCCCTCCTCCACCTCCGCGCTAACTCTCTCCGCCGCCGACACATACTGGCGGCTATCCCTTCCGCCGACGGCCAATCGCGACCACCCATGGAGCAACCCACTGGTGAATGGCGGCGCAAAGCTGAAAGAAATGAAACTTCCACCATTAATGGTATGCATATCGGAGTTGGACATACTGTTGGACAGAAACTTAGAATTCTGTGCAGCCATGGCAGGCGCCGGGAAGAAAGTGGAGAGAGTGATTCTGAAGGGAGTTGGACATGGCTTCCAAATTCTGAACAAAGATCAGATCTCCACTCAGCGAACACAAGAACTGATGGGTTCCATTCGAGCTTTCATTCATCAACAATAG

Protein sequence

MIFKRMAAMYIDPRLKLQMAKPHLQNGGTSEEIEGLIRVYADGHVERPPIIPNVACSTTASDKGVTAKDVLIDIFTNTWARIYIPNSQRNLPLLVYFHGGGFCVGSAAWSCYHEFLLNLASKAKCVILSINYRLSPENRLPAAYDDGFNALMWLKQQAITNPIEHKWWLGNCNPSKLFLAGDSAGANISYNTATRIGHNESTLLKPLTLKGIILIQPFFGGESRTKSEKLAATPSSTSALTLSAADTYWRLSLPPTANRDHPWSNPLVNGGAKLKEMKLPPLMVCISELDILLDRNLEFCAAMAGAGKKVERVILKGVGHGFQILNKDQISTQRTQELMGSIRAFIHQQ
Homology
BLAST of CmoCh01G004720 vs. ExPASy Swiss-Prot
Match: Q9LFR7 (Probable carboxylesterase 17 OS=Arabidopsis thaliana OX=3702 GN=CXE17 PE=1 SV=1)

HSP 1 Score: 344.0 bits (881), Expect = 2.0e-93
Identity = 180/330 (54.55%), Postives = 223/330 (67.58%), Query Frame = 0

Query: 23  HLQNGGTSEEIEGLIRVYADGHVERPPIIPNVACSTTASDKGVTAKDVLIDIFTNTWARI 82
           H ++G   EEIEGLI+V+ DG VERPPI+P V+ +   S K  TA D  I +  +TW R+
Sbjct: 22  HHRHGPVVEEIEGLIKVFNDGCVERPPIVPIVSPTIHPSSK-ATAFD--IKLSNDTWTRV 81

Query: 83  YIPNSQR-----NLPLLVYFHGGGFCVGSAAWSCYHEFLLNLASKAKCVILSINYRLSPE 142
           YIP++        LPLLVYFHGGGFCVGSAAWSCYH+FL +LA KA+CVI+S+NYRL+PE
Sbjct: 82  YIPDAAAASPSVTLPLLVYFHGGGFCVGSAAWSCYHDFLTSLAVKARCVIVSVNYRLAPE 141

Query: 143 NRLPAAYDDGFNALMWLKQQAITNPIEHKWWLGNCNPSKLFLAGDSAGANISYNTATRIG 202
           +RLPAAYDDG N + WL +Q I+    +  WL  CN S +FLAGDSAGANI+Y  A RI 
Sbjct: 142 HRLPAAYDDGVNVVSWLVKQQISTGGGYPSWLSKCNLSNVFLAGDSAGANIAYQVAVRI- 201

Query: 203 HNESTLLKPLTLKGIILIQPFFGGESRTKSEKLAATPSSTSALTLSAADTYWRLSLPPTA 262
                    L LKGIILI PFFGGESRT SEK     + +SALTLSA+D YWRL+LP  A
Sbjct: 202 MASGKYANTLHLKGIILIHPFFGGESRTSSEK-QQHHTKSSALTLSASDAYWRLALPRGA 261

Query: 263 NRDHPWSNPLVNGGAKLKEMKLPPLMVCISELDILLDRNLEFCAAMAGAGKKVERVILKG 322
           +RDHPW NPL++        KLP  MV ++E DIL +RNLE C  M   GK+VE ++  G
Sbjct: 262 SRDHPWCNPLMSSAG----AKLPTTMVFMAEFDILKERNLEMCKVMRSHGKRVEGIVHGG 321

Query: 323 VGHGFQILNKDQISTQRTQELMGSIRAFIH 348
           VGH F IL+   +S  R  ++M  +  FIH
Sbjct: 322 VGHAFHILDNSSVSRDRIHDMMCRLHNFIH 342

BLAST of CmoCh01G004720 vs. ExPASy Swiss-Prot
Match: Q9SX25 (Probable carboxylesterase 6 OS=Arabidopsis thaliana OX=3702 GN=CXE6 PE=2 SV=1)

HSP 1 Score: 305.8 bits (782), Expect = 6.1e-82
Identity = 153/327 (46.79%), Postives = 215/327 (65.75%), Query Frame = 0

Query: 26  NGGTSEEIEGLIRVYADGHVERPPIIPNVACSTTASDKGVTAKDVLIDIFTNTWARIYIP 85
           +G   +E+EGLI+VY DGHVER  ++P V  S    + GVT  DV+ID  TN WAR+Y+P
Sbjct: 20  HGPVVDEVEGLIKVYKDGHVERSQLLPCVDPSLPL-ELGVTCSDVVIDKLTNVWARLYVP 79

Query: 86  -----NSQRNLPLLVYFHGGGFCVGSAAWSCYHEFLLNLASKAKCVILSINYRLSPENRL 145
                +S   LPL+VYFHGGGFCVGSA+W CYHEFL  L+++++C+++S+NYRL+PEN L
Sbjct: 80  MTTTKSSVSKLPLIVYFHGGGFCVGSASWLCYHEFLARLSARSRCLVMSVNYRLAPENPL 139

Query: 146 PAAYDDGFNALMWLKQQAITNPIEHKWWLGNCNPSKLFLAGDSAGANISYNTATRIGHNE 205
           PAAY+DG NA++WL +    N      W   C+  ++FLAGDSAG NI+   A R+   E
Sbjct: 140 PAAYEDGVNAILWLNKARNDN-----LWAKQCDFGRIFLAGDSAGGNIAQQVAARLASPE 199

Query: 206 STLLKPLTLKGIILIQPFFGGESRTKSEKLAATPSSTSALTLSAADTYWRLSLPPTANRD 265
              LK   ++G ILIQPF+ GE RT+SE+       T+ LTL+++D +WR+SLP  ANR+
Sbjct: 200 DLALK---IEGTILIQPFYSGEERTESERRVGN-DKTAVLTLASSDAWWRMSLPRGANRE 259

Query: 266 HPWSNPLVNGGAKLKEMKLPPLMVCISELDILLDRNLEFCAAMAGAGKKVERVILKGVGH 325
           HP+  P+      +K   +   +VC++E+D+L+D N+E C    G    ++RV+ KGVGH
Sbjct: 260 HPYCKPV---KMIIKSSTVTRTLVCVAEMDLLMDSNMEMC---DGNEDVIKRVLHKGVGH 319

Query: 326 GFQILNKDQISTQRTQELMGSIRAFIH 348
            F IL K Q++   T E++  I AFIH
Sbjct: 320 AFHILGKSQLAHTTTLEMLCQIDAFIH 330

BLAST of CmoCh01G004720 vs. ExPASy Swiss-Prot
Match: Q9FG13 (Probable carboxylesterase 15 OS=Arabidopsis thaliana OX=3702 GN=CXE15 PE=2 SV=1)

HSP 1 Score: 191.0 bits (484), Expect = 2.2e-47
Identity = 109/299 (36.45%), Postives = 171/299 (57.19%), Query Frame = 0

Query: 30  SEEIEGLIRVYADGHVERPPIIPNVACSTT-ASDKGVTAKDVLIDIFTNTWARIYIPNSQ 89
           +E+  GL+++ ++G V R   I  +       +++ V  KD +     N   R+Y P S 
Sbjct: 11  AEDCMGLLQLLSNGTVLRSESIDLITQQIPFKNNQTVLFKDSIYHKPNNLHLRLYKPISA 70

Query: 90  RN---LPLLVYFHGGGFCVGSAAWSCYHEFLLNLASKAKCVILSINYRLSPENRLPAAYD 149
            N   LP++V+FHGGGFC GS +W  +H F L LAS    +++S +YRL+PE+RLPAA++
Sbjct: 71  SNRTALPVVVFFHGGGFCFGSRSWPHFHNFCLTLASSLNALVVSPDYRLAPEHRLPAAFE 130

Query: 150 DGFNALMWLKQQAITNPIEHKWWLG-NCNPSKLFLAGDSAGANISYNTATRIGHNESTLL 209
           D    L WL  QA+++ + H +  G + +  ++F+ GDS+G NI++  A R G + S  L
Sbjct: 131 DAEAVLTWLWDQAVSDGVNHWFEDGTDVDFDRVFVVGDSSGGNIAHQLAVRFG-SGSIEL 190

Query: 210 KPLTLKGIILIQPFFGGESRTKSEKLAATPSSTSALTLSAADTYWRLSLPPTANRDHPWS 269
            P+ ++G +L+ PFFGGE RT SE       S + L+L   D +WRLSLP  A RDH  +
Sbjct: 191 TPVRVRGYVLMGPFFGGEERTNSE----NGPSEALLSLDLLDKFWRLSLPNGATRDHHMA 250

Query: 270 NPLVNGGAKLKEMKLPPLMVCISELDILLDRNLEFCAAMAG-AGKKVERVILKGVGHGF 323
           NP       L+ + L P++V +   ++L DR  E+   +    GK+V+ +  +   HGF
Sbjct: 251 NPFGPTSPTLESISLEPMLVIVGGSELLRDRAKEYAYKLKKMGGKRVDYIEFENKEHGF 304

BLAST of CmoCh01G004720 vs. ExPASy Swiss-Prot
Match: Q0ZPV7 (Carboxylesterase 1 OS=Actinidia eriantha OX=165200 GN=CXE1 PE=1 SV=1)

HSP 1 Score: 174.5 bits (441), Expect = 2.1e-42
Identity = 110/306 (35.95%), Postives = 155/306 (50.65%), Query Frame = 0

Query: 19  MAKPHLQNGGTSEEIEGLIR-----VYADGHVERPPIIPNVACS-TTASDKGVTAKDVLI 78
           M+  HL+  G+S+    L++     +  D  + RP  IP+ A S    S   V  KD+ +
Sbjct: 1   MSNDHLETTGSSDPNTNLLKYLPIVLNPDRTITRPIQIPSTAASPDPTSSSPVLTKDLAL 60

Query: 79  DIFTNTWARIYIPN----SQRNLPLLVYFHGGGFCVGSAAWSCYHEFLLNLASKAKCVIL 138
           +   NT+ R+++P     +   LPL+VYFHGGGF + SAA + +H+F   +A  A  VI 
Sbjct: 61  NPLHNTFVRLFLPRHALYNSAKLPLVVYFHGGGFILFSAASTIFHDFCCEMAVHAGVVIA 120

Query: 139 SINYRLSPENRLPAAYDDGFNALMWLKQQAITNPIEHKWWLGNCNPSKLFLAGDSAGANI 198
           S++YRL+PE+RLPAAYDD   AL W+K          +W     + S  F+ G+SAG NI
Sbjct: 121 SVDYRLAPEHRLPAAYDDAMEALQWIKDS------RDEWLTNFADFSNCFIMGESAGGNI 180

Query: 199 SYNTATRIGHNESTLLKPLTLKGIILIQPFFGGESRTKSEKLAATPSSTSALTLSAADTY 258
           +Y+   R       LL PL +KG++L +P FGG  RT SE   A  S      L   D  
Sbjct: 181 AYHAGLRAAAVADELL-PLKIKGLVLDEPGFGGSKRTGSELRLANDSRLPTFVL---DLI 240

Query: 259 WRLSLPPTANRDHPWSNPLVNGGAKLKEMKLPPL----MVCISELDILLDRNLEFCAAMA 311
           W LSLP  A+RDH + NP           K+  L    MV     D ++DR +E    + 
Sbjct: 241 WELSLPMGADRDHEYCNPTAESEPLYSFDKIRSLGWRVMVVGCHGDPMIDRQMELAERLE 296

BLAST of CmoCh01G004720 vs. ExPASy Swiss-Prot
Match: Q9LVB8 (Probable carboxylesterase 120 OS=Arabidopsis thaliana OX=3702 GN=CXE20 PE=2 SV=1)

HSP 1 Score: 167.9 bits (424), Expect = 2.0e-40
Identity = 107/321 (33.33%), Postives = 162/321 (50.47%), Query Frame = 0

Query: 42  DGHVERPPIIPNVACSTTASDKG----VTAKDVLIDIFTNTWARIYIPNSQRN------- 101
           DG + R   + N  C+    D        +KD+ ++   +TW R+Y+P+S  N       
Sbjct: 21  DGSITRD--LSNFPCTAATPDPSPLNPAVSKDLPVNQLKSTWLRLYLPSSAVNEGNVSSQ 80

Query: 102 -LPLLVYFHGGGFCVGSAAWSCYHEFLLNLASKAKCVILSINYRLSPENRLPAAYDDGFN 161
            LP++VY+HGGGF + S     +H+F   +A     +++S +YRL+PE+RLPAAYDDG  
Sbjct: 81  KLPIVVYYHGGGFILCSVDMQLFHDFCSEVARDLNAIVVSPSYRLAPEHRLPAAYDDGVE 140

Query: 162 ALMWLKQQAITNPIEHKWWLGNCNPSKLFLAGDSAGANISYNTATRIGHNESTLLKPLTL 221
           AL W+K        + +W   + + S +FL G SAG N++YN   R   + S  L PL +
Sbjct: 141 ALDWIKTS------DDEWIKSHADFSNVFLMGTSAGGNLAYNVGLRSVDSVSD-LSPLQI 200

Query: 222 KGIILIQPFFGGESRTKSEKLAATPSSTSALTLSAADTYWRLSLPPTANRDHPWSNPLVN 281
           +G+IL  PFFGGE R++SE           +     D  W LSLP   +RDH +SNP V 
Sbjct: 201 RGLILHHPFFGGEERSESEIRLMNDQVCPPI---VTDVMWDLSLPVGVDRDHEYSNPTVG 260

Query: 282 GGA----KLKEMKLPPLMVCISELDILLDRNLEFCAAMAGAGKKVERVILKGVGHGFQIL 341
            G+    K+  ++   +M+   E D ++D   +    M   G +V      G  HG +I 
Sbjct: 261 DGSEKLEKIGRLRWKVMMIG-GEDDPMIDLQKDVAKLMKKKGVEVVEHYTGGHVHGAEIR 320

Query: 342 NKDQISTQRTQELMGSIRAFI 347
           +  +  T     L  SI+ FI
Sbjct: 321 DPSKRKT-----LFLSIKNFI 323

BLAST of CmoCh01G004720 vs. ExPASy TrEMBL
Match: A0A6J1G8X8 (probable carboxylesterase 17 OS=Cucurbita moschata OX=3662 GN=LOC111452001 PE=4 SV=1)

HSP 1 Score: 716.8 bits (1849), Expect = 4.3e-203
Identity = 349/349 (100.00%), Postives = 349/349 (100.00%), Query Frame = 0

Query: 1   MIFKRMAAMYIDPRLKLQMAKPHLQNGGTSEEIEGLIRVYADGHVERPPIIPNVACSTTA 60
           MIFKRMAAMYIDPRLKLQMAKPHLQNGGTSEEIEGLIRVYADGHVERPPIIPNVACSTTA
Sbjct: 1   MIFKRMAAMYIDPRLKLQMAKPHLQNGGTSEEIEGLIRVYADGHVERPPIIPNVACSTTA 60

Query: 61  SDKGVTAKDVLIDIFTNTWARIYIPNSQRNLPLLVYFHGGGFCVGSAAWSCYHEFLLNLA 120
           SDKGVTAKDVLIDIFTNTWARIYIPNSQRNLPLLVYFHGGGFCVGSAAWSCYHEFLLNLA
Sbjct: 61  SDKGVTAKDVLIDIFTNTWARIYIPNSQRNLPLLVYFHGGGFCVGSAAWSCYHEFLLNLA 120

Query: 121 SKAKCVILSINYRLSPENRLPAAYDDGFNALMWLKQQAITNPIEHKWWLGNCNPSKLFLA 180
           SKAKCVILSINYRLSPENRLPAAYDDGFNALMWLKQQAITNPIEHKWWLGNCNPSKLFLA
Sbjct: 121 SKAKCVILSINYRLSPENRLPAAYDDGFNALMWLKQQAITNPIEHKWWLGNCNPSKLFLA 180

Query: 181 GDSAGANISYNTATRIGHNESTLLKPLTLKGIILIQPFFGGESRTKSEKLAATPSSTSAL 240
           GDSAGANISYNTATRIGHNESTLLKPLTLKGIILIQPFFGGESRTKSEKLAATPSSTSAL
Sbjct: 181 GDSAGANISYNTATRIGHNESTLLKPLTLKGIILIQPFFGGESRTKSEKLAATPSSTSAL 240

Query: 241 TLSAADTYWRLSLPPTANRDHPWSNPLVNGGAKLKEMKLPPLMVCISELDILLDRNLEFC 300
           TLSAADTYWRLSLPPTANRDHPWSNPLVNGGAKLKEMKLPPLMVCISELDILLDRNLEFC
Sbjct: 241 TLSAADTYWRLSLPPTANRDHPWSNPLVNGGAKLKEMKLPPLMVCISELDILLDRNLEFC 300

Query: 301 AAMAGAGKKVERVILKGVGHGFQILNKDQISTQRTQELMGSIRAFIHQQ 350
           AAMAGAGKKVERVILKGVGHGFQILNKDQISTQRTQELMGSIRAFIHQQ
Sbjct: 301 AAMAGAGKKVERVILKGVGHGFQILNKDQISTQRTQELMGSIRAFIHQQ 349

BLAST of CmoCh01G004720 vs. ExPASy TrEMBL
Match: A0A6J1KCZ3 (probable carboxylesterase 17 OS=Cucurbita maxima OX=3661 GN=LOC111493192 PE=4 SV=1)

HSP 1 Score: 692.6 bits (1786), Expect = 8.6e-196
Identity = 335/344 (97.38%), Postives = 341/344 (99.13%), Query Frame = 0

Query: 6   MAAMYIDPRLKLQMAKPHLQNGGTSEEIEGLIRVYADGHVERPPIIPNVACSTTASDKGV 65
           MAAMYIDPRLKLQMAKPHLQNG TSEEIEGLIRVYADGHVERPPIIPNVACSTTAS+KGV
Sbjct: 1   MAAMYIDPRLKLQMAKPHLQNGATSEEIEGLIRVYADGHVERPPIIPNVACSTTASNKGV 60

Query: 66  TAKDVLIDIFTNTWARIYIPNSQRNLPLLVYFHGGGFCVGSAAWSCYHEFLLNLASKAKC 125
           TAKDVLIDIFTNTWARIYIPNSQRNLPLLVYFHGGGFCVGSAAWSCYHEFLLNLASKAKC
Sbjct: 61  TAKDVLIDIFTNTWARIYIPNSQRNLPLLVYFHGGGFCVGSAAWSCYHEFLLNLASKAKC 120

Query: 126 VILSINYRLSPENRLPAAYDDGFNALMWLKQQAITNPIEHKWWLGNCNPSKLFLAGDSAG 185
           VILSINYRLSPENRLPAAY+DGFNA+MWLKQQAITNPIEHKWWLGNCNPSKLFLAGDSAG
Sbjct: 121 VILSINYRLSPENRLPAAYEDGFNAVMWLKQQAITNPIEHKWWLGNCNPSKLFLAGDSAG 180

Query: 186 ANISYNTATRIGHNESTLLKPLTLKGIILIQPFFGGESRTKSEKLAATPSSTSALTLSAA 245
           ANI+YNTATR+GHNESTLLKP TLKGIILIQPFFGGESRTKSEKLAATPSSTSALTLSA+
Sbjct: 181 ANIAYNTATRLGHNESTLLKPPTLKGIILIQPFFGGESRTKSEKLAATPSSTSALTLSAS 240

Query: 246 DTYWRLSLPPTANRDHPWSNPLVNGGAKLKEMKLPPLMVCISELDILLDRNLEFCAAMAG 305
           DTYWRLSLPPTANRDHPWSNPLVNGGAKLKEMKLPPLMVCISELDILLDRNLEFCAAMA 
Sbjct: 241 DTYWRLSLPPTANRDHPWSNPLVNGGAKLKEMKLPPLMVCISELDILLDRNLEFCAAMAA 300

Query: 306 AGKKVERVILKGVGHGFQILNKDQISTQRTQELMGSIRAFIHQQ 350
           AGKKVERVILKGVGHGFQILNKDQISTQRTQELMGSIRAFIHQQ
Sbjct: 301 AGKKVERVILKGVGHGFQILNKDQISTQRTQELMGSIRAFIHQQ 344

BLAST of CmoCh01G004720 vs. ExPASy TrEMBL
Match: A0A6J1DL90 (probable carboxylesterase 17 OS=Momordica charantia OX=3673 GN=LOC111022166 PE=4 SV=1)

HSP 1 Score: 607.4 bits (1565), Expect = 3.6e-170
Identity = 298/348 (85.63%), Postives = 314/348 (90.23%), Query Frame = 0

Query: 1   MIFKRMAAMYIDPRLKLQMAKPHLQNGGTSEEIEGLIRVYADGHVERPPIIPNVACSTTA 60
           M FKRMAA+ IDPRLKLQM K HLQ   T+EEIEGLIRVYADGHVERPPIIPNV CSTTA
Sbjct: 1   MKFKRMAAISIDPRLKLQMPKSHLQCASTAEEIEGLIRVYADGHVERPPIIPNVPCSTTA 60

Query: 61  SDKGVTAKDVLIDIFTNTWARIYIPNSQRNLPLLVYFHGGGFCVGSAAWSCYHEFLLNLA 120
           S+KGV+AKDVLID FTNTWARIYI NSQR LPLLVYFHGGGFCVGSAAWSCYHEFLL+LA
Sbjct: 61  SNKGVSAKDVLIDNFTNTWARIYIANSQRKLPLLVYFHGGGFCVGSAAWSCYHEFLLDLA 120

Query: 121 SKAKCVILSINYRLSPENRLPAAYDDGFNALMWLKQQAITNPIEHKWWLGNCNPSKLFLA 180
           SKAKC ILSINYRLSPENRLPAAY+DGFNALMWLKQQAI+ P EHKWWLGNCNPS LFLA
Sbjct: 121 SKAKCAILSINYRLSPENRLPAAYEDGFNALMWLKQQAISEPNEHKWWLGNCNPSNLFLA 180

Query: 181 GDSAGANISYNTATRIGHNESTLLKPLTLKGIILIQPFFGGESRTKSEKLAATPSSTSAL 240
           GDSAGANI+YN ATR G NE   LKPLTLKG+ILIQPFFGGESRTKSEKLAA P STSAL
Sbjct: 181 GDSAGANIAYNIATRPGLNEPATLKPLTLKGVILIQPFFGGESRTKSEKLAAQP-STSAL 240

Query: 241 TLSAADTYWRLSLPPTANRDHPWSNPLVNGGAKLKEMKLPPLMVCISELDILLDRNLEFC 300
           TL+ ADTYWRLSLPP A+RDHPWSNPLVNGG KL++ KLPP MVCISELDIL+DRNLEFC
Sbjct: 241 TLATADTYWRLSLPPGASRDHPWSNPLVNGGTKLRDTKLPPSMVCISELDILVDRNLEFC 300

Query: 301 AAMAGAGKKVERVILKGVGHGFQILNKDQISTQRTQELMGSIRAFIHQ 349
           AAMA AGKKVERVI KGVGHGFQILNKDQIS  RTQE+M SI+ FIHQ
Sbjct: 301 AAMAAAGKKVERVIFKGVGHGFQILNKDQISAIRTQEMMVSIQNFIHQ 347

BLAST of CmoCh01G004720 vs. ExPASy TrEMBL
Match: A0A2N9EQ59 (Abhydrolase_3 domain-containing protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS4850 PE=4 SV=1)

HSP 1 Score: 476.1 bits (1224), Expect = 1.3e-130
Identity = 228/346 (65.90%), Postives = 274/346 (79.19%), Query Frame = 0

Query: 6   MAAMYIDPRLKLQMAKPHLQNGGTSEEIEGLIRVYADGHVERPPIIPNVACSTTASDKGV 65
           MAA+ +DPR KLQ+ K H Q+GG  EEIEGLI+V  DGHVERPPI+P V C T A ++GV
Sbjct: 1   MAAISLDPRFKLQVGKNHHQHGGVVEEIEGLIKVCMDGHVERPPIVPIVPC-TMALERGV 60

Query: 66  TAKDVLIDIFTNTWARIYIPNSQRNLPLLVYFHGGGFCVGSAAWSCYHEFLLNLASKAKC 125
           TA DV+ID  T+ WARIY+P+    LPLLVYFHGGGFCVGSAAWSCY+EFL NLASKA C
Sbjct: 61  TANDVVIDKCTSLWARIYVPSCSGKLPLLVYFHGGGFCVGSAAWSCYNEFLSNLASKANC 120

Query: 126 VILSINYRLSPENRLPAAYDDGFNALMWLKQQAITNPIEHKWWLGNCNPSKLFLAGDSAG 185
           VI+S+NYRL+PENRLPAAY+DGFN LMW+KQQA+    EHKWWL +CN + +FL GDS+G
Sbjct: 121 VIISVNYRLAPENRLPAAYEDGFNTLMWVKQQALNGSNEHKWWLRHCNLTSMFLGGDSSG 180

Query: 186 ANISYNTATRIGH---NESTLLKPLTLKGIILIQPFFGGESRTKSEKLAATPSSTSALTL 245
           ANI+YN ATR+G    +EST+++PL LKG ILIQPFFGGE+RT SEK    P++ S LTL
Sbjct: 181 ANIAYNVATRLGSSGTSESTIIRPLCLKGTILIQPFFGGEARTWSEKYTNQPAN-SGLTL 240

Query: 246 SAADTYWRLSLPPTANRDHPWSNPLVNGGAKLKEMKLPPLMVCISELDILLDRNLEFCAA 305
             +DTYWRLSLP   NRD+PW NPL NG AKL+E +LP +MVC+SE DIL DRNLEFC A
Sbjct: 241 LVSDTYWRLSLPIGTNRDNPWCNPLANGMAKLREFRLPSIMVCVSETDILKDRNLEFCTA 300

Query: 306 MAGAGKKVERVILKGVGHGFQILNKDQISTQRTQELMGSIRAFIHQ 349
           +  AGK+VE VI KGVGH FQIL+  Q+S  RT E+M  I+AFI++
Sbjct: 301 LTNAGKRVETVIYKGVGHAFQILHNSQLSQPRTHEMMAHIKAFINE 344

BLAST of CmoCh01G004720 vs. ExPASy TrEMBL
Match: F6HSF1 (Abhydrolase_3 domain-containing protein OS=Vitis vinifera OX=29760 GN=VIT_14s0006g03180 PE=4 SV=1)

HSP 1 Score: 466.8 bits (1200), Expect = 7.7e-128
Identity = 225/344 (65.41%), Postives = 269/344 (78.20%), Query Frame = 0

Query: 6   MAAMYIDPRLKLQMAKPHLQNGGTSEEIEGLIRVYADGHVERPPIIPNVACSTTASDKGV 65
           MAA+ +DPRL LQM K   QNG   E++EGLIRVY DGHVERP I+PNV C T A + GV
Sbjct: 1   MAAISLDPRLSLQMGKNLYQNGVVVEKVEGLIRVYNDGHVERPAIVPNVPC-TVALELGV 60

Query: 66  TAKDVLIDIFTNTWARIYIPNSQR-NLPLLVYFHGGGFCVGSAAWSCYHEFLLNLASKAK 125
           T KDV+I+ ++N WAR Y+P+     LPLLVYFHGGGFCVGSAAW+CYH FL +LASKA 
Sbjct: 61  TVKDVVIEKYSNLWARFYVPSCPAGKLPLLVYFHGGGFCVGSAAWNCYHGFLADLASKAG 120

Query: 126 CVILSINYRLSPENRLPAAYDDGFNALMWLKQQAITNPIEHKWWLGNCNPSKLFLAGDSA 185
           C+I+S+NYRL+PENRLPAAY+DGFNA+MW+K QA+    E KWWL  CN S LFL GDSA
Sbjct: 121 CLIMSVNYRLAPENRLPAAYEDGFNAVMWVKNQALNGAGEQKWWLSRCNLSSLFLTGDSA 180

Query: 186 GANISYNTATRIGHNESTLLKPLTLKGIILIQPFFGGESRTKSEKLAATPSSTSALTLSA 245
           GANI+YN ATR+G +++T LKPL+LKG ILIQPFFGGE+RT SE  +  P + SALTLSA
Sbjct: 181 GANIAYNVATRLGSSDTTFLKPLSLKGTILIQPFFGGEARTGSENHSTQPPN-SALTLSA 240

Query: 246 ADTYWRLSLPPTANRDHPWSNPLVNGGAKLKEMKLPPLMVCISELDILLDRNLEFCAAMA 305
           +DTYWRLSLP  ANRDHP  NPL NG  KL+ ++LPP MVCIS+ DIL DRNL+FC AMA
Sbjct: 241 SDTYWRLSLPLGANRDHPCCNPLANGSTKLRTLQLPPTMVCISDTDILKDRNLQFCTAMA 300

Query: 306 GAGKKVERVILKGVGHGFQILNKDQISTQRTQELMGSIRAFIHQ 349
            AGK++E VI KGVGH FQ+L    +S  RT+E++  IRAFI Q
Sbjct: 301 NAGKRLETVIYKGVGHAFQVLQNSDLSQPRTKEMISHIRAFITQ 342

BLAST of CmoCh01G004720 vs. NCBI nr
Match: XP_022948283.1 (probable carboxylesterase 17 [Cucurbita moschata])

HSP 1 Score: 716.8 bits (1849), Expect = 8.8e-203
Identity = 349/349 (100.00%), Postives = 349/349 (100.00%), Query Frame = 0

Query: 1   MIFKRMAAMYIDPRLKLQMAKPHLQNGGTSEEIEGLIRVYADGHVERPPIIPNVACSTTA 60
           MIFKRMAAMYIDPRLKLQMAKPHLQNGGTSEEIEGLIRVYADGHVERPPIIPNVACSTTA
Sbjct: 1   MIFKRMAAMYIDPRLKLQMAKPHLQNGGTSEEIEGLIRVYADGHVERPPIIPNVACSTTA 60

Query: 61  SDKGVTAKDVLIDIFTNTWARIYIPNSQRNLPLLVYFHGGGFCVGSAAWSCYHEFLLNLA 120
           SDKGVTAKDVLIDIFTNTWARIYIPNSQRNLPLLVYFHGGGFCVGSAAWSCYHEFLLNLA
Sbjct: 61  SDKGVTAKDVLIDIFTNTWARIYIPNSQRNLPLLVYFHGGGFCVGSAAWSCYHEFLLNLA 120

Query: 121 SKAKCVILSINYRLSPENRLPAAYDDGFNALMWLKQQAITNPIEHKWWLGNCNPSKLFLA 180
           SKAKCVILSINYRLSPENRLPAAYDDGFNALMWLKQQAITNPIEHKWWLGNCNPSKLFLA
Sbjct: 121 SKAKCVILSINYRLSPENRLPAAYDDGFNALMWLKQQAITNPIEHKWWLGNCNPSKLFLA 180

Query: 181 GDSAGANISYNTATRIGHNESTLLKPLTLKGIILIQPFFGGESRTKSEKLAATPSSTSAL 240
           GDSAGANISYNTATRIGHNESTLLKPLTLKGIILIQPFFGGESRTKSEKLAATPSSTSAL
Sbjct: 181 GDSAGANISYNTATRIGHNESTLLKPLTLKGIILIQPFFGGESRTKSEKLAATPSSTSAL 240

Query: 241 TLSAADTYWRLSLPPTANRDHPWSNPLVNGGAKLKEMKLPPLMVCISELDILLDRNLEFC 300
           TLSAADTYWRLSLPPTANRDHPWSNPLVNGGAKLKEMKLPPLMVCISELDILLDRNLEFC
Sbjct: 241 TLSAADTYWRLSLPPTANRDHPWSNPLVNGGAKLKEMKLPPLMVCISELDILLDRNLEFC 300

Query: 301 AAMAGAGKKVERVILKGVGHGFQILNKDQISTQRTQELMGSIRAFIHQQ 350
           AAMAGAGKKVERVILKGVGHGFQILNKDQISTQRTQELMGSIRAFIHQQ
Sbjct: 301 AAMAGAGKKVERVILKGVGHGFQILNKDQISTQRTQELMGSIRAFIHQQ 349

BLAST of CmoCh01G004720 vs. NCBI nr
Match: XP_023523365.1 (probable carboxylesterase 17 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 706.4 bits (1822), Expect = 1.2e-199
Identity = 342/349 (97.99%), Postives = 346/349 (99.14%), Query Frame = 0

Query: 1   MIFKRMAAMYIDPRLKLQMAKPHLQNGGTSEEIEGLIRVYADGHVERPPIIPNVACSTTA 60
           MIFKRMAAMY+DPRLKLQMAKPHLQNGGTSEEIEGLIRVYADGHVERPPIIPNVACSTTA
Sbjct: 1   MIFKRMAAMYVDPRLKLQMAKPHLQNGGTSEEIEGLIRVYADGHVERPPIIPNVACSTTA 60

Query: 61  SDKGVTAKDVLIDIFTNTWARIYIPNSQRNLPLLVYFHGGGFCVGSAAWSCYHEFLLNLA 120
           SDKGVTAKDVLIDIFTNTWARIYIPNSQRNLPLLVYFHGGGFCVGSAAWSCYHEFLLNLA
Sbjct: 61  SDKGVTAKDVLIDIFTNTWARIYIPNSQRNLPLLVYFHGGGFCVGSAAWSCYHEFLLNLA 120

Query: 121 SKAKCVILSINYRLSPENRLPAAYDDGFNALMWLKQQAITNPIEHKWWLGNCNPSKLFLA 180
           SKAKCVILSINYRLSPENRLPAAYDDGFNALMWLKQQAITNPIEHKWW GNCNPSKLFLA
Sbjct: 121 SKAKCVILSINYRLSPENRLPAAYDDGFNALMWLKQQAITNPIEHKWWSGNCNPSKLFLA 180

Query: 181 GDSAGANISYNTATRIGHNESTLLKPLTLKGIILIQPFFGGESRTKSEKLAATPSSTSAL 240
           GDSAGANI YNTATR+GHNESTLLKPLTLKGIILIQPFFGGESRTKSEKLAATPSSTSAL
Sbjct: 181 GDSAGANIVYNTATRLGHNESTLLKPLTLKGIILIQPFFGGESRTKSEKLAATPSSTSAL 240

Query: 241 TLSAADTYWRLSLPPTANRDHPWSNPLVNGGAKLKEMKLPPLMVCISELDILLDRNLEFC 300
           TLSA+DTYWRLSLPPTANRDHPWSNPLVNGGAKLKE KLPPLMVCISELDILLDRNLEFC
Sbjct: 241 TLSASDTYWRLSLPPTANRDHPWSNPLVNGGAKLKETKLPPLMVCISELDILLDRNLEFC 300

Query: 301 AAMAGAGKKVERVILKGVGHGFQILNKDQISTQRTQELMGSIRAFIHQQ 350
           AAMAGAGKKV+RVILKGVGHGFQILNKDQISTQRTQELMGSIRAFIHQQ
Sbjct: 301 AAMAGAGKKVDRVILKGVGHGFQILNKDQISTQRTQELMGSIRAFIHQQ 349

BLAST of CmoCh01G004720 vs. NCBI nr
Match: KAG6607091.1 (putative carboxylesterase 17, partial [Cucurbita argyrosperma subsp. sororia] >KAG7036780.1 putative carboxylesterase 17, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 695.3 bits (1793), Expect = 2.7e-196
Identity = 338/344 (98.26%), Postives = 341/344 (99.13%), Query Frame = 0

Query: 6   MAAMYIDPRLKLQMAKPHLQNGGTSEEIEGLIRVYADGHVERPPIIPNVACSTTASDKGV 65
           MAAMYIDPRLKLQMAKPHLQNGGTSEEIEGLIRVYADGHVERPPIIPNVACSTTASDKGV
Sbjct: 1   MAAMYIDPRLKLQMAKPHLQNGGTSEEIEGLIRVYADGHVERPPIIPNVACSTTASDKGV 60

Query: 66  TAKDVLIDIFTNTWARIYIPNSQRNLPLLVYFHGGGFCVGSAAWSCYHEFLLNLASKAKC 125
           TAKDVLIDIFTNTWARIYIPNSQRNLPLLVYFHGGGFCVGSAAWSCYHEFLLNLASKAKC
Sbjct: 61  TAKDVLIDIFTNTWARIYIPNSQRNLPLLVYFHGGGFCVGSAAWSCYHEFLLNLASKAKC 120

Query: 126 VILSINYRLSPENRLPAAYDDGFNALMWLKQQAITNPIEHKWWLGNCNPSKLFLAGDSAG 185
           VILSINYRLSPENRLPAAYDDGFNALMWLKQQAITN IEHKWWLGNCNPSKLFLAGDSAG
Sbjct: 121 VILSINYRLSPENRLPAAYDDGFNALMWLKQQAITNAIEHKWWLGNCNPSKLFLAGDSAG 180

Query: 186 ANISYNTATRIGHNESTLLKPLTLKGIILIQPFFGGESRTKSEKLAATPSSTSALTLSAA 245
           ANI+YNTATR+GHNESTLLKPLTLKGIILIQPFFGGESRTKSEKLAATPSSTSALTLSA+
Sbjct: 181 ANIAYNTATRLGHNESTLLKPLTLKGIILIQPFFGGESRTKSEKLAATPSSTSALTLSAS 240

Query: 246 DTYWRLSLPPTANRDHPWSNPLVNGGAKLKEMKLPPLMVCISELDILLDRNLEFCAAMAG 305
           DTYWRLSLPPTANRDHPWSNPLVNG AKLKEMKLPPLMV ISELDILLDRNLEFCAAMAG
Sbjct: 241 DTYWRLSLPPTANRDHPWSNPLVNGSAKLKEMKLPPLMVSISELDILLDRNLEFCAAMAG 300

Query: 306 AGKKVERVILKGVGHGFQILNKDQISTQRTQELMGSIRAFIHQQ 350
           AGKKVERVILKGVGHGFQILNKDQISTQRTQELMGSIRAFIHQQ
Sbjct: 301 AGKKVERVILKGVGHGFQILNKDQISTQRTQELMGSIRAFIHQQ 344

BLAST of CmoCh01G004720 vs. NCBI nr
Match: XP_022998605.1 (probable carboxylesterase 17 [Cucurbita maxima])

HSP 1 Score: 692.6 bits (1786), Expect = 1.8e-195
Identity = 335/344 (97.38%), Postives = 341/344 (99.13%), Query Frame = 0

Query: 6   MAAMYIDPRLKLQMAKPHLQNGGTSEEIEGLIRVYADGHVERPPIIPNVACSTTASDKGV 65
           MAAMYIDPRLKLQMAKPHLQNG TSEEIEGLIRVYADGHVERPPIIPNVACSTTAS+KGV
Sbjct: 1   MAAMYIDPRLKLQMAKPHLQNGATSEEIEGLIRVYADGHVERPPIIPNVACSTTASNKGV 60

Query: 66  TAKDVLIDIFTNTWARIYIPNSQRNLPLLVYFHGGGFCVGSAAWSCYHEFLLNLASKAKC 125
           TAKDVLIDIFTNTWARIYIPNSQRNLPLLVYFHGGGFCVGSAAWSCYHEFLLNLASKAKC
Sbjct: 61  TAKDVLIDIFTNTWARIYIPNSQRNLPLLVYFHGGGFCVGSAAWSCYHEFLLNLASKAKC 120

Query: 126 VILSINYRLSPENRLPAAYDDGFNALMWLKQQAITNPIEHKWWLGNCNPSKLFLAGDSAG 185
           VILSINYRLSPENRLPAAY+DGFNA+MWLKQQAITNPIEHKWWLGNCNPSKLFLAGDSAG
Sbjct: 121 VILSINYRLSPENRLPAAYEDGFNAVMWLKQQAITNPIEHKWWLGNCNPSKLFLAGDSAG 180

Query: 186 ANISYNTATRIGHNESTLLKPLTLKGIILIQPFFGGESRTKSEKLAATPSSTSALTLSAA 245
           ANI+YNTATR+GHNESTLLKP TLKGIILIQPFFGGESRTKSEKLAATPSSTSALTLSA+
Sbjct: 181 ANIAYNTATRLGHNESTLLKPPTLKGIILIQPFFGGESRTKSEKLAATPSSTSALTLSAS 240

Query: 246 DTYWRLSLPPTANRDHPWSNPLVNGGAKLKEMKLPPLMVCISELDILLDRNLEFCAAMAG 305
           DTYWRLSLPPTANRDHPWSNPLVNGGAKLKEMKLPPLMVCISELDILLDRNLEFCAAMA 
Sbjct: 241 DTYWRLSLPPTANRDHPWSNPLVNGGAKLKEMKLPPLMVCISELDILLDRNLEFCAAMAA 300

Query: 306 AGKKVERVILKGVGHGFQILNKDQISTQRTQELMGSIRAFIHQQ 350
           AGKKVERVILKGVGHGFQILNKDQISTQRTQELMGSIRAFIHQQ
Sbjct: 301 AGKKVERVILKGVGHGFQILNKDQISTQRTQELMGSIRAFIHQQ 344

BLAST of CmoCh01G004720 vs. NCBI nr
Match: XP_022155020.1 (probable carboxylesterase 17 [Momordica charantia])

HSP 1 Score: 607.4 bits (1565), Expect = 7.5e-170
Identity = 298/348 (85.63%), Postives = 314/348 (90.23%), Query Frame = 0

Query: 1   MIFKRMAAMYIDPRLKLQMAKPHLQNGGTSEEIEGLIRVYADGHVERPPIIPNVACSTTA 60
           M FKRMAA+ IDPRLKLQM K HLQ   T+EEIEGLIRVYADGHVERPPIIPNV CSTTA
Sbjct: 1   MKFKRMAAISIDPRLKLQMPKSHLQCASTAEEIEGLIRVYADGHVERPPIIPNVPCSTTA 60

Query: 61  SDKGVTAKDVLIDIFTNTWARIYIPNSQRNLPLLVYFHGGGFCVGSAAWSCYHEFLLNLA 120
           S+KGV+AKDVLID FTNTWARIYI NSQR LPLLVYFHGGGFCVGSAAWSCYHEFLL+LA
Sbjct: 61  SNKGVSAKDVLIDNFTNTWARIYIANSQRKLPLLVYFHGGGFCVGSAAWSCYHEFLLDLA 120

Query: 121 SKAKCVILSINYRLSPENRLPAAYDDGFNALMWLKQQAITNPIEHKWWLGNCNPSKLFLA 180
           SKAKC ILSINYRLSPENRLPAAY+DGFNALMWLKQQAI+ P EHKWWLGNCNPS LFLA
Sbjct: 121 SKAKCAILSINYRLSPENRLPAAYEDGFNALMWLKQQAISEPNEHKWWLGNCNPSNLFLA 180

Query: 181 GDSAGANISYNTATRIGHNESTLLKPLTLKGIILIQPFFGGESRTKSEKLAATPSSTSAL 240
           GDSAGANI+YN ATR G NE   LKPLTLKG+ILIQPFFGGESRTKSEKLAA P STSAL
Sbjct: 181 GDSAGANIAYNIATRPGLNEPATLKPLTLKGVILIQPFFGGESRTKSEKLAAQP-STSAL 240

Query: 241 TLSAADTYWRLSLPPTANRDHPWSNPLVNGGAKLKEMKLPPLMVCISELDILLDRNLEFC 300
           TL+ ADTYWRLSLPP A+RDHPWSNPLVNGG KL++ KLPP MVCISELDIL+DRNLEFC
Sbjct: 241 TLATADTYWRLSLPPGASRDHPWSNPLVNGGTKLRDTKLPPSMVCISELDILVDRNLEFC 300

Query: 301 AAMAGAGKKVERVILKGVGHGFQILNKDQISTQRTQELMGSIRAFIHQ 349
           AAMA AGKKVERVI KGVGHGFQILNKDQIS  RTQE+M SI+ FIHQ
Sbjct: 301 AAMAAAGKKVERVIFKGVGHGFQILNKDQISAIRTQEMMVSIQNFIHQ 347

BLAST of CmoCh01G004720 vs. TAIR 10
Match: AT5G16080.1 (carboxyesterase 17 )

HSP 1 Score: 344.0 bits (881), Expect = 1.4e-94
Identity = 180/330 (54.55%), Postives = 223/330 (67.58%), Query Frame = 0

Query: 23  HLQNGGTSEEIEGLIRVYADGHVERPPIIPNVACSTTASDKGVTAKDVLIDIFTNTWARI 82
           H ++G   EEIEGLI+V+ DG VERPPI+P V+ +   S K  TA D  I +  +TW R+
Sbjct: 22  HHRHGPVVEEIEGLIKVFNDGCVERPPIVPIVSPTIHPSSK-ATAFD--IKLSNDTWTRV 81

Query: 83  YIPNSQR-----NLPLLVYFHGGGFCVGSAAWSCYHEFLLNLASKAKCVILSINYRLSPE 142
           YIP++        LPLLVYFHGGGFCVGSAAWSCYH+FL +LA KA+CVI+S+NYRL+PE
Sbjct: 82  YIPDAAAASPSVTLPLLVYFHGGGFCVGSAAWSCYHDFLTSLAVKARCVIVSVNYRLAPE 141

Query: 143 NRLPAAYDDGFNALMWLKQQAITNPIEHKWWLGNCNPSKLFLAGDSAGANISYNTATRIG 202
           +RLPAAYDDG N + WL +Q I+    +  WL  CN S +FLAGDSAGANI+Y  A RI 
Sbjct: 142 HRLPAAYDDGVNVVSWLVKQQISTGGGYPSWLSKCNLSNVFLAGDSAGANIAYQVAVRI- 201

Query: 203 HNESTLLKPLTLKGIILIQPFFGGESRTKSEKLAATPSSTSALTLSAADTYWRLSLPPTA 262
                    L LKGIILI PFFGGESRT SEK     + +SALTLSA+D YWRL+LP  A
Sbjct: 202 MASGKYANTLHLKGIILIHPFFGGESRTSSEK-QQHHTKSSALTLSASDAYWRLALPRGA 261

Query: 263 NRDHPWSNPLVNGGAKLKEMKLPPLMVCISELDILLDRNLEFCAAMAGAGKKVERVILKG 322
           +RDHPW NPL++        KLP  MV ++E DIL +RNLE C  M   GK+VE ++  G
Sbjct: 262 SRDHPWCNPLMSSAG----AKLPTTMVFMAEFDILKERNLEMCKVMRSHGKRVEGIVHGG 321

Query: 323 VGHGFQILNKDQISTQRTQELMGSIRAFIH 348
           VGH F IL+   +S  R  ++M  +  FIH
Sbjct: 322 VGHAFHILDNSSVSRDRIHDMMCRLHNFIH 342

BLAST of CmoCh01G004720 vs. TAIR 10
Match: AT1G68620.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 305.8 bits (782), Expect = 4.3e-83
Identity = 153/327 (46.79%), Postives = 215/327 (65.75%), Query Frame = 0

Query: 26  NGGTSEEIEGLIRVYADGHVERPPIIPNVACSTTASDKGVTAKDVLIDIFTNTWARIYIP 85
           +G   +E+EGLI+VY DGHVER  ++P V  S    + GVT  DV+ID  TN WAR+Y+P
Sbjct: 20  HGPVVDEVEGLIKVYKDGHVERSQLLPCVDPSLPL-ELGVTCSDVVIDKLTNVWARLYVP 79

Query: 86  -----NSQRNLPLLVYFHGGGFCVGSAAWSCYHEFLLNLASKAKCVILSINYRLSPENRL 145
                +S   LPL+VYFHGGGFCVGSA+W CYHEFL  L+++++C+++S+NYRL+PEN L
Sbjct: 80  MTTTKSSVSKLPLIVYFHGGGFCVGSASWLCYHEFLARLSARSRCLVMSVNYRLAPENPL 139

Query: 146 PAAYDDGFNALMWLKQQAITNPIEHKWWLGNCNPSKLFLAGDSAGANISYNTATRIGHNE 205
           PAAY+DG NA++WL +    N      W   C+  ++FLAGDSAG NI+   A R+   E
Sbjct: 140 PAAYEDGVNAILWLNKARNDN-----LWAKQCDFGRIFLAGDSAGGNIAQQVAARLASPE 199

Query: 206 STLLKPLTLKGIILIQPFFGGESRTKSEKLAATPSSTSALTLSAADTYWRLSLPPTANRD 265
              LK   ++G ILIQPF+ GE RT+SE+       T+ LTL+++D +WR+SLP  ANR+
Sbjct: 200 DLALK---IEGTILIQPFYSGEERTESERRVGN-DKTAVLTLASSDAWWRMSLPRGANRE 259

Query: 266 HPWSNPLVNGGAKLKEMKLPPLMVCISELDILLDRNLEFCAAMAGAGKKVERVILKGVGH 325
           HP+  P+      +K   +   +VC++E+D+L+D N+E C    G    ++RV+ KGVGH
Sbjct: 260 HPYCKPV---KMIIKSSTVTRTLVCVAEMDLLMDSNMEMC---DGNEDVIKRVLHKGVGH 319

Query: 326 GFQILNKDQISTQRTQELMGSIRAFIH 348
            F IL K Q++   T E++  I AFIH
Sbjct: 320 AFHILGKSQLAHTTTLEMLCQIDAFIH 330

BLAST of CmoCh01G004720 vs. TAIR 10
Match: AT5G06570.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 191.0 bits (484), Expect = 1.6e-48
Identity = 109/299 (36.45%), Postives = 171/299 (57.19%), Query Frame = 0

Query: 30  SEEIEGLIRVYADGHVERPPIIPNVACSTT-ASDKGVTAKDVLIDIFTNTWARIYIPNSQ 89
           +E+  GL+++ ++G V R   I  +       +++ V  KD +     N   R+Y P S 
Sbjct: 11  AEDCMGLLQLLSNGTVLRSESIDLITQQIPFKNNQTVLFKDSIYHKPNNLHLRLYKPISA 70

Query: 90  RN---LPLLVYFHGGGFCVGSAAWSCYHEFLLNLASKAKCVILSINYRLSPENRLPAAYD 149
            N   LP++V+FHGGGFC GS +W  +H F L LAS    +++S +YRL+PE+RLPAA++
Sbjct: 71  SNRTALPVVVFFHGGGFCFGSRSWPHFHNFCLTLASSLNALVVSPDYRLAPEHRLPAAFE 130

Query: 150 DGFNALMWLKQQAITNPIEHKWWLG-NCNPSKLFLAGDSAGANISYNTATRIGHNESTLL 209
           D    L WL  QA+++ + H +  G + +  ++F+ GDS+G NI++  A R G + S  L
Sbjct: 131 DAEAVLTWLWDQAVSDGVNHWFEDGTDVDFDRVFVVGDSSGGNIAHQLAVRFG-SGSIEL 190

Query: 210 KPLTLKGIILIQPFFGGESRTKSEKLAATPSSTSALTLSAADTYWRLSLPPTANRDHPWS 269
            P+ ++G +L+ PFFGGE RT SE       S + L+L   D +WRLSLP  A RDH  +
Sbjct: 191 TPVRVRGYVLMGPFFGGEERTNSE----NGPSEALLSLDLLDKFWRLSLPNGATRDHHMA 250

Query: 270 NPLVNGGAKLKEMKLPPLMVCISELDILLDRNLEFCAAMAG-AGKKVERVILKGVGHGF 323
           NP       L+ + L P++V +   ++L DR  E+   +    GK+V+ +  +   HGF
Sbjct: 251 NPFGPTSPTLESISLEPMLVIVGGSELLRDRAKEYAYKLKKMGGKRVDYIEFENKEHGF 304

BLAST of CmoCh01G004720 vs. TAIR 10
Match: AT5G06570.2 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 191.0 bits (484), Expect = 1.6e-48
Identity = 109/299 (36.45%), Postives = 171/299 (57.19%), Query Frame = 0

Query: 30  SEEIEGLIRVYADGHVERPPIIPNVACSTT-ASDKGVTAKDVLIDIFTNTWARIYIPNSQ 89
           +E+  GL+++ ++G V R   I  +       +++ V  KD +     N   R+Y P S 
Sbjct: 11  AEDCMGLLQLLSNGTVLRSESIDLITQQIPFKNNQTVLFKDSIYHKPNNLHLRLYKPISA 70

Query: 90  RN---LPLLVYFHGGGFCVGSAAWSCYHEFLLNLASKAKCVILSINYRLSPENRLPAAYD 149
            N   LP++V+FHGGGFC GS +W  +H F L LAS    +++S +YRL+PE+RLPAA++
Sbjct: 71  SNRTALPVVVFFHGGGFCFGSRSWPHFHNFCLTLASSLNALVVSPDYRLAPEHRLPAAFE 130

Query: 150 DGFNALMWLKQQAITNPIEHKWWLG-NCNPSKLFLAGDSAGANISYNTATRIGHNESTLL 209
           D    L WL  QA+++ + H +  G + +  ++F+ GDS+G NI++  A R G + S  L
Sbjct: 131 DAEAVLTWLWDQAVSDGVNHWFEDGTDVDFDRVFVVGDSSGGNIAHQLAVRFG-SGSIEL 190

Query: 210 KPLTLKGIILIQPFFGGESRTKSEKLAATPSSTSALTLSAADTYWRLSLPPTANRDHPWS 269
            P+ ++G +L+ PFFGGE RT SE       S + L+L   D +WRLSLP  A RDH  +
Sbjct: 191 TPVRVRGYVLMGPFFGGEERTNSE----NGPSEALLSLDLLDKFWRLSLPNGATRDHHMA 250

Query: 270 NPLVNGGAKLKEMKLPPLMVCISELDILLDRNLEFCAAMAG-AGKKVERVILKGVGHGF 323
           NP       L+ + L P++V +   ++L DR  E+   +    GK+V+ +  +   HGF
Sbjct: 251 NPFGPTSPTLESISLEPMLVIVGGSELLRDRAKEYAYKLKKMGGKRVDYIEFENKEHGF 304

BLAST of CmoCh01G004720 vs. TAIR 10
Match: AT5G62180.1 (carboxyesterase 20 )

HSP 1 Score: 167.9 bits (424), Expect = 1.4e-41
Identity = 107/321 (33.33%), Postives = 162/321 (50.47%), Query Frame = 0

Query: 42  DGHVERPPIIPNVACSTTASDKG----VTAKDVLIDIFTNTWARIYIPNSQRN------- 101
           DG + R   + N  C+    D        +KD+ ++   +TW R+Y+P+S  N       
Sbjct: 21  DGSITRD--LSNFPCTAATPDPSPLNPAVSKDLPVNQLKSTWLRLYLPSSAVNEGNVSSQ 80

Query: 102 -LPLLVYFHGGGFCVGSAAWSCYHEFLLNLASKAKCVILSINYRLSPENRLPAAYDDGFN 161
            LP++VY+HGGGF + S     +H+F   +A     +++S +YRL+PE+RLPAAYDDG  
Sbjct: 81  KLPIVVYYHGGGFILCSVDMQLFHDFCSEVARDLNAIVVSPSYRLAPEHRLPAAYDDGVE 140

Query: 162 ALMWLKQQAITNPIEHKWWLGNCNPSKLFLAGDSAGANISYNTATRIGHNESTLLKPLTL 221
           AL W+K        + +W   + + S +FL G SAG N++YN   R   + S  L PL +
Sbjct: 141 ALDWIKTS------DDEWIKSHADFSNVFLMGTSAGGNLAYNVGLRSVDSVSD-LSPLQI 200

Query: 222 KGIILIQPFFGGESRTKSEKLAATPSSTSALTLSAADTYWRLSLPPTANRDHPWSNPLVN 281
           +G+IL  PFFGGE R++SE           +     D  W LSLP   +RDH +SNP V 
Sbjct: 201 RGLILHHPFFGGEERSESEIRLMNDQVCPPI---VTDVMWDLSLPVGVDRDHEYSNPTVG 260

Query: 282 GGA----KLKEMKLPPLMVCISELDILLDRNLEFCAAMAGAGKKVERVILKGVGHGFQIL 341
            G+    K+  ++   +M+   E D ++D   +    M   G +V      G  HG +I 
Sbjct: 261 DGSEKLEKIGRLRWKVMMIG-GEDDPMIDLQKDVAKLMKKKGVEVVEHYTGGHVHGAEIR 320

Query: 342 NKDQISTQRTQELMGSIRAFI 347
           +  +  T     L  SI+ FI
Sbjct: 321 DPSKRKT-----LFLSIKNFI 323

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9LFR72.0e-9354.55Probable carboxylesterase 17 OS=Arabidopsis thaliana OX=3702 GN=CXE17 PE=1 SV=1[more]
Q9SX256.1e-8246.79Probable carboxylesterase 6 OS=Arabidopsis thaliana OX=3702 GN=CXE6 PE=2 SV=1[more]
Q9FG132.2e-4736.45Probable carboxylesterase 15 OS=Arabidopsis thaliana OX=3702 GN=CXE15 PE=2 SV=1[more]
Q0ZPV72.1e-4235.95Carboxylesterase 1 OS=Actinidia eriantha OX=165200 GN=CXE1 PE=1 SV=1[more]
Q9LVB82.0e-4033.33Probable carboxylesterase 120 OS=Arabidopsis thaliana OX=3702 GN=CXE20 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1G8X84.3e-203100.00probable carboxylesterase 17 OS=Cucurbita moschata OX=3662 GN=LOC111452001 PE=4 ... [more]
A0A6J1KCZ38.6e-19697.38probable carboxylesterase 17 OS=Cucurbita maxima OX=3661 GN=LOC111493192 PE=4 SV... [more]
A0A6J1DL903.6e-17085.63probable carboxylesterase 17 OS=Momordica charantia OX=3673 GN=LOC111022166 PE=4... [more]
A0A2N9EQ591.3e-13065.90Abhydrolase_3 domain-containing protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS... [more]
F6HSF17.7e-12865.41Abhydrolase_3 domain-containing protein OS=Vitis vinifera OX=29760 GN=VIT_14s000... [more]
Match NameE-valueIdentityDescription
XP_022948283.18.8e-203100.00probable carboxylesterase 17 [Cucurbita moschata][more]
XP_023523365.11.2e-19997.99probable carboxylesterase 17 [Cucurbita pepo subsp. pepo][more]
KAG6607091.12.7e-19698.26putative carboxylesterase 17, partial [Cucurbita argyrosperma subsp. sororia] >K... [more]
XP_022998605.11.8e-19597.38probable carboxylesterase 17 [Cucurbita maxima][more]
XP_022155020.17.5e-17085.63probable carboxylesterase 17 [Momordica charantia][more]
Match NameE-valueIdentityDescription
AT5G16080.11.4e-9454.55carboxyesterase 17 [more]
AT1G68620.14.3e-8346.79alpha/beta-Hydrolases superfamily protein [more]
AT5G06570.11.6e-4836.45alpha/beta-Hydrolases superfamily protein [more]
AT5G06570.21.6e-4836.45alpha/beta-Hydrolases superfamily protein [more]
AT5G62180.11.4e-4133.33carboxyesterase 20 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR029058Alpha/Beta hydrolase foldGENE3D3.40.50.1820alpha/beta hydrolasecoord: 39..348
e-value: 2.9E-77
score: 262.2
IPR029058Alpha/Beta hydrolase foldSUPERFAMILY53474alpha/beta-Hydrolasescoord: 35..348
IPR013094Alpha/beta hydrolase fold-3PFAMPF07859Abhydrolase_3coord: 94..323
e-value: 9.6E-57
score: 192.2
NoneNo IPR availablePANTHERPTHR23024ARYLACETAMIDE DEACETYLASEcoord: 20..347
NoneNo IPR availablePANTHERPTHR23024:SF409CARBOXYLESTERASE 6-RELATEDcoord: 20..347

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh01G004720.1CmoCh01G004720.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0016787 hydrolase activity