CmoCh01G004000 (gene) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh01G004000
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
DescriptionMannose-6-phosphate isomerase
LocationCmo_Chr01: 1959326 .. 1964770 (+)
RNA-Seq ExpressionCmoCh01G004000
SyntenyCmoCh01G004000
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAGTCTGACCCTGTTTTGACGCAGAAACAGAGCAATGTCCGCCGATTAAAGTGCTCCGTTCAAAATTACGATTGGGGAATCAGAGGACACGATTCCCTCGTCGCCAGGCTCTTCGCCTTAAACTCCGGATCGTCTGTCGCTTCCCATAAACCCTACGCCGAGTTCTGGATGGGGACTCATGATTCCGGACCGTCGTTTGTAATCGCCGCCGCCGCCGATAATGGTGGTGCAATCGACTCCGATTCCATTTCCTTGAAGTCCTGGGTTTTGGAAAACCCTAATGTCCTCGGCGATAAGGTTGTTGAGAAATGGGGGTCTGATATCCCCTTTCTGTTCAAGGTACGGCGTTGATTGATCAAACGGGTGATTGTATGTTGTTCTTCCTCATTTGGGTTTTCGTTTCTTTAGGTTCTTTCTGTGGCGAAACCATTGTCGATTCAGGCTCATCCGGATAAGGAATTGGCGAGATTACTTCATAAGGTGGAGCCTAAAGTTTATAGAGATGCGAATCACAAGCCTGAAATGGCATTGGCCATTACAGAGTTCGAGGCTCTGTGTGGATTCATCAGTGTGGAGGTAAATTATTTGGTGACAACGTCAAATCCTCCATGGATGATTAAATTTTGATTTGTTTTGTTTTGTTTTGTTTTGTTTGCAGGAGCTGAAGGATGTTCTTCGTTCTGTTCCTGAGATTTTAGTGATGATTCGTGATGCAGACAGAAACAGAGTGTTGTTGTTAGATGATCCTGCAAATAAAGAAGCGGACCTTAAGGCTGCTCTGAGGTTAGCTTTCACCCAGTTAATGTCGGCTTCCAAAATTTTGATATCCAGAGCCATATCCGACTTGAAAAGGCGACTGCATTCTGAAAGTGAGGTAGCTATGCTATGTTTTATTTGGTTTCTTCTTCACGTGATCTGTTCTTTTTGGCTATAGAATTTAGGAACCACTGTTTATCTCTGGCCATAACTCTTCATTCTCTGTTACTGTGGCGTTGAAGAACTTGTAGTTGTGTTTGCATATGCTCTGTAGTATTGTTTTCTTTGAAAAAAAGATAGTAGAGATGTAGAGAACCACGGCTATTAGATATTGTCCCCTTTGGAGATAGTAGATATTGACTTCTTTGAGCTTTCACTTCTAGTTTCTCTGGGTTTCTTCAACCTAGGTGGGATCTCACAATCCACCCTCCTTGGGGGGCTAGCGTCCTCGCTGGCACACCGCCTGGTGTTTGACTCTAATATCATTTATAGCAGCTCCTCACAATCCACTCTCCTTGGGGGCCAGCGTCCTTGCTGGCACATGTTTGGCTCTAACATCATTTATAACAGCATAAGTTCACCAATAATAGATATTATTCTCTTTGGGTACATTGCCAAGTGTTTAGCTTTGATACCATTTATAACACCTCAAGTCCACCACTAAACAGATATTATTCTCTTTGGGCTTTTCCTTCGAGACTTTCTCTCAAGGTTTTAAAACGTGTCTATTAGGGAGAGGTTTCCACCTTGTAAGGAATGCTTCGTTCTCCTCTCTAACCAACGTAGGATCTCACACTTCAACAGGGCAGGATATTGCCCTGTTGTTCAAGTTTGAAGTACAATTGACAACGGCCAGACTTTTGGAGTATAACATTCATGATTTGTTCATTTAATCTTTCTTCAGGTGAGGCAACTAACTGCTAAGGAACTTTTGGTTGTACGTCTGGAGAAGCAATATCCTGATGATGTTGGCATCATAGCAGCCTTTTTCTTAAATCATGTGAAGCTTAATCCTGGAGAAGCTCTGTACCTTGGGGCAAATGAGCCTCATGCATATATAAGCGGCGAGTGTATCGAGTGCATGGCAACATCTGATAACGTCATCCGGGCTGGCCTTACTCCCAAGTTTCGCGATGTCCATACTCTCTGTGCCATGTTTACTTACAAACAGGTGAACTTCTCCACTGAACCATTACAACCATTTCCCTAACCAAACTTCATATCAACTTCAAGCAACAATACAGCGTTCTTGGATGTTCATCCATCGACAACCGAAAAAGTTACCCTTATTTTCTTGGATCTGCAGGGCTTCCCTGAAATCCTCCAAGGAGTTCATGTGAATCAATACATAACAAAGTATGTTCCACCATTTGACGAATTCGAGGTCGATCGATGTGTTCTTCCCAAGGGTGCATCTGTCTCATTTCCCGCGAGCCAAGGTCCTTCGATATTTCTGGTTGCTGGAGGAGAGGGGAGGATGCTGCCTCAAGAATCACTTAGAAGTGATGTAATCAGTGAAGGAGACGTCCTCTTTGTACCTGCTAAAACTGGGATTAACATAACAAGTAATGTTGAGTTGCTCATTTTCAGAGCAGGAGTAAACAACAAGTTCTTTTAATACATACATACACAAATGGTAAATAAAAGATTCACCTTCTTCAGGAAAAGACTCATCCAGATCCTCCAACTTCATCTACCTCCAAAGCATACTCATTTTACAAATTCGCATTTACTTCACCAAATTATACAGAACTATGAAATAATTAAAATGATTCTCTTAAAAAGTGAAGCCTATCAGAAGGCAGCAGCAATAATGGATGCAAGATCTGATTCAAGAAAGAGACCTTTTCTCAGCTAGTAATGTCATTTCCATCATTTTCATTCCAAGGAGAGCACTAATCATCCAACCCTTTGCCAGTTTCCAAGAAATTCACTATATCCTGTCTTAGAAGCATTTGAAAAATTATCAAAATGGTAGGAAAATTCTGAATAGTAAGGATTACCTGTGCTACTTTACTTGCCTTTTGCTTCATTCCATCAGTAATCTGTTCCTTCTGGAAAGTATGAATTTTCGAAGAAAGGAGGATTTGTGTACTTATCTTCATTTTTTTCCTCTTTATGGCTTTATTACTTCTTTACACGCAAATTTTGATCATTCCCTCTCTCTTTTCACGAACAGTAGTAAATCAAACCAATCGTAGATGCTTGCATTTGCCAATCAATAGTAGATGACATGTACATCCTTATCATGGACCCTGCTTATCAGAGACTTTCAACATCAGAAAATGAGGCATATCACAACTGCTAGCTATCTAGCAATAATCTTCATTTACTTAATTTTGAAGGAAAACGAATTACATCCACAAACAAATGGTAAGTCGATTTAAATTCGATTTAAATGATTCATATAAGTAGTTAAGCCTGCAAGCAAACTTTCAGAAGCTCAATCATGATAGAGTTTAGACAAGACCTGCATCACTTCAAGTCCTCCAAAGCATCTGAAGAAAGAGGTTTGAATATGGAAGTCATAAACCAGTTCCCAAATATGACAGAGAGCATGTTAGAGGACTTCAATGAAACAGACTTCTCAGAAGAAAACTTCTTGGGCTACCATTTCCAGTTACCAGAAAACACAACACGGAGCTCAATTTCTGATTTTCCTCGTGTTGCCCTGTTCAAATGTCAGAATGAGTTTCCGGTGATAACTCGAAGCAGTCCACTGGTAGATAGCTCTCAAGAAACCATGAATAGAAAAGCAAAACAAGTATTGGTAAGCTTCTCAGGAACCCCTTGTTCAGCTTTTGGTAATAACTGTAGCGTTGCTAGGAATTTGAAGGCAAGTGTCAATTCTTCATCAAATTCTGTCTTTGAGTATTTGATGTGTGTTCTTATTGGTTTCTTTGTTTACTCAGAACTTAGGAGGAAGGAAAAGAAGGAAATGTGAGAAAGAACAAGAAAAGCCTGCAGAAGTAGTTCATATTAGAGCAAAAAGAGGTGAAGCTACCCATAGTCACAGCTTAGCAGAAAGGGTATATTACTGAATTATACAATCCATCTTCCTAATACAAGTCGTTTTAAGGATTGATATTTCAGGTGAAATGAAAAATCCAATACTGAAACCATTTTTCATATTTCAGGTGAGAAGGGAGAAAATCAACAACAAATTAAAATGCTTACAAAACCTTGTTCCAGGTTGCCACAAGGTAATTTCTTTTCCTAGTCCTGTTTACCAAAACATTGAAGTCTCAAGTTCAAATCAAACTTCCATGGTATTCTGCAGACAATGGGTATGACAATGGTGTTGGATGAGACAATCACTTATGTTCATTCACTACAGAATCAGGTTGAGGTGAGGAATATGGTGCACTTTTCATAAGTATTTGTAATGTTTTCATCAAATAGCACTGATCTATTCACTTTGTTCCTGAATTCAGTTTCTCTCAATGGAGCTTGCAACTGCCTGTTCCACTTTAGGCATAAATTCTGGCATGGGAGCTACCAGAAAAGCTTCGGTAATCCAACAACTTCCTGAAAGAGTATTAACTTTTGACATTTTACTTTCTAATATTAAAAATAAAACATAAATTTACTGCAAGTATGGAATTGATATTGATGCATCCTTTATTAATTAGTGTTGAAATATTGACGTTTAAGCTTAAAGCATAATATAGCAGGGGACAAGATCACATGAGGAACAGGAGGAGGAAGAGAAATAAATAAGCAAATGATAAAGAGAGCACAGTTGGTTCCACTAGAGTAAGCCCTTTTGACTGTGGGGCATTTGTTATCTGTTCTCATGAATCTGCTTCCTATATTCACTTTCTTTCTAGGCAAACAAGATTGGTTTCTCATAAGTCCACAAAATGCTGTAAGCAAAACTACCAGACAGTGATGTATCAATTCTACATCTGCTGTAAATTGCAACCAAATACCTAATATTGTCTAGAACAGTTTCTTTGTGGGATTTTGTTACCTTGTCTGTAAATCATGTCTACATGTTCAAAAAAACTGATGAAATAACGGAGATCCTTGCAAGACTGTGATTCTTTCAACATTGCACAGTTATCTCTTGTTCTAAGCCAAATAGCTTAGGCTCTTTGAAGTTCAAATGAAAGGCAACTATATTAGCAAATGCTCCGATCGCACAATAACACACACTCGATCTAGATGAATACAAATAACATAAGAGAGAAAAGCATCTTAGACATTGCAGATTTCATCAGGTCTCTCCTATAGTATGTCCAAATCATAGGATCTGGACCAATTCCCGATACTCTCTAAAATCCAAAATCCCTCAAAGCGTAATACATTATGACACCTCCCAATGGCTCATCTCCATCACGTAAGGAGGATAAGAAGTAGTGTTTAAAGGGCAACCACCAGCTATGACGAACAGAGAAACAAATTATGGAATACCCCACATATAAATTGAACAAGAACAAAAGAAAACAGACAGAAACTATAAGGGGGCTAGCCATGGCCCTACGTACAAACGATAAGCAAAAGATCCCAAGGCCATTACCGATTAAAGAGAATAAAACTGTGGGGAATAGTCATTGGGGCATTTCAGCGAATACCTTGCATACATGGAAGTGGGGTTTCGTGGGCATTGCCATTGCTACATGTGACGAAGCAAATCAAACTCAAATCAAATTCAGATATTCTCATCCTCAGGTAGAATATTAA

mRNA sequence

ATGGAGTCTGACCCTGTTTTGACGCAGAAACAGAGCAATGTCCGCCGATTAAAGTGCTCCGTTCAAAATTACGATTGGGGAATCAGAGGACACGATTCCCTCGTCGCCAGGCTCTTCGCCTTAAACTCCGGATCGTCTGTCGCTTCCCATAAACCCTACGCCGAGTTCTGGATGGGGACTCATGATTCCGGACCGTCGTTTGTAATCGCCGCCGCCGCCGATAATGGTGGTGCAATCGACTCCGATTCCATTTCCTTGAAGTCCTGGGTTTTGGAAAACCCTAATGTCCTCGGCGATAAGGTTGTTGAGAAATGGGGGTCTGATATCCCCTTTCTGTTCAAGGTTCTTTCTGTGGCGAAACCATTGTCGATTCAGGCTCATCCGGATAAGGAATTGGCGAGATTACTTCATAAGGTGGAGCCTAAAGTTTATAGAGATGCGAATCACAAGCCTGAAATGGCATTGGCCATTACAGAGTTCGAGGCTCTGTGTGGATTCATCAGTGTGGAGGAGCTGAAGGATGTTCTTCGTTCTGTTCCTGAGATTTTAGTGATGATTCGTGATGCAGACAGAAACAGAGTGTTGTTGTTAGATGATCCTGCAAATAAAGAAGCGGACCTTAAGGCTGCTCTGAGGTTAGCTTTCACCCAGTTAATGTCGGCTTCCAAAATTTTGATATCCAGAGCCATATCCGACTTGAAAAGGCGACTGCATTCTGAAAGTGAGGTGAGGCAACTAACTGCTAAGGAACTTTTGGTTGTACGTCTGGAGAAGCAATATCCTGATGATGTTGGCATCATAGCAGCCTTTTTCTTAAATCATGTGAAGCTTAATCCTGGAGAAGCTCTGTACCTTGGGGCAAATGAGCCTCATGCATATATAAGCGGCGAGTGTATCGAGTGCATGGCAACATCTGATAACGTCATCCGGGCTGGCCTTACTCCCAAGTTTCGCGATGTCCATACTCTCTGTGCCATGTTTACTTACAAACAGGGCTTCCCTGAAATCCTCCAAGGAGTTCATGTGAATCAATACATAACAAAGTATGTTCCACCATTTGACGAATTCGAGGTCGATCGATGTGTTCTTCCCAAGGGTGCATCTGTCTCATTTCCCGCGAGCCAAGGTCCTTCGATATTTCTGGTTGCTGGAGGAGAGGGGAGGATGCTGCCTCAAGAATCACTTAGAAGTGATGTAATCAGTGAAGGAGACGTCCTCTTTGTACCTGCTAAAACTGGGATTAACATAACAAGTAATGTTGAGTTGCTCATTTTCAGAGCAGGAGAAAACGAATTACATCCACAAACAAATGCCTGCAAGCAAACTTTCAGAAGCTCAATCATGATAGAGTTTAGACAAGACCTGCATCACTTCAAGTCCTCCAAAGCATCTGAAGAAAGAGGTTTGAATATGGAAGTCATAAACCAGTTCCCAAATATGACAGAGAGCATGTTAGAGGACTTCAATGAAACAGACTTCTCAGAAGAAAACTTCTTGGGCTACCATTTCCAGTTACCAGAAAACACAACACGGAGCTCAATTTCTGATTTTCCTCGTGTTGCCCTGTTCAAATGTCAGAATGAGTTTCCGGTGATAACTCGAAGCAGTCCACTGGTAGATAGCTCTCAAGAAACCATGAATAGAAAAGCAAAACAAGTATTGAACTTAGGAGGAAGGAAAAGAAGGAAATGTGAGAAAGAACAAGAAAAGCCTGCAGAAGTAGTTCATATTAGAGCAAAAAGAGGTGAAGCTACCCATAGTCACAGCTTAGCAGAAAGGGTGAGAAGGGAGAAAATCAACAACAAATTAAAATGCTTACAAAACCTTGTTCCAGGTTGCCACAAGACAATGGGTATGACAATGGTGTTGGATGAGACAATCACTTATGTTCATTCACTACAGAATCAGGTTGAGTTTCTCTCAATGGAGCTTGCAACTGCCTGTTCCACTTTAGGCATAAATTCTGGCATGGGAGCTACCAGAAAAGCTTCGACAGAAACTATAAGGGGGCTAGCCATGGCCCTACGTACAAACGATAAGCAAAAGATCCCAAGGCCATTACCGATTAAAGAGAATAAAACTGTGGGGAATAGTCATTGGGGCATTTCAGCGAATACCTTGCATACATGGAAGTGGGGTTTCGTGGGCATTGCCATTGCTACATGTGACGAAGCAAATCAAACTCAAATCAAATTCAGATATTCTCATCCTCAGGTAGAATATTAA

Coding sequence (CDS)

ATGGAGTCTGACCCTGTTTTGACGCAGAAACAGAGCAATGTCCGCCGATTAAAGTGCTCCGTTCAAAATTACGATTGGGGAATCAGAGGACACGATTCCCTCGTCGCCAGGCTCTTCGCCTTAAACTCCGGATCGTCTGTCGCTTCCCATAAACCCTACGCCGAGTTCTGGATGGGGACTCATGATTCCGGACCGTCGTTTGTAATCGCCGCCGCCGCCGATAATGGTGGTGCAATCGACTCCGATTCCATTTCCTTGAAGTCCTGGGTTTTGGAAAACCCTAATGTCCTCGGCGATAAGGTTGTTGAGAAATGGGGGTCTGATATCCCCTTTCTGTTCAAGGTTCTTTCTGTGGCGAAACCATTGTCGATTCAGGCTCATCCGGATAAGGAATTGGCGAGATTACTTCATAAGGTGGAGCCTAAAGTTTATAGAGATGCGAATCACAAGCCTGAAATGGCATTGGCCATTACAGAGTTCGAGGCTCTGTGTGGATTCATCAGTGTGGAGGAGCTGAAGGATGTTCTTCGTTCTGTTCCTGAGATTTTAGTGATGATTCGTGATGCAGACAGAAACAGAGTGTTGTTGTTAGATGATCCTGCAAATAAAGAAGCGGACCTTAAGGCTGCTCTGAGGTTAGCTTTCACCCAGTTAATGTCGGCTTCCAAAATTTTGATATCCAGAGCCATATCCGACTTGAAAAGGCGACTGCATTCTGAAAGTGAGGTGAGGCAACTAACTGCTAAGGAACTTTTGGTTGTACGTCTGGAGAAGCAATATCCTGATGATGTTGGCATCATAGCAGCCTTTTTCTTAAATCATGTGAAGCTTAATCCTGGAGAAGCTCTGTACCTTGGGGCAAATGAGCCTCATGCATATATAAGCGGCGAGTGTATCGAGTGCATGGCAACATCTGATAACGTCATCCGGGCTGGCCTTACTCCCAAGTTTCGCGATGTCCATACTCTCTGTGCCATGTTTACTTACAAACAGGGCTTCCCTGAAATCCTCCAAGGAGTTCATGTGAATCAATACATAACAAAGTATGTTCCACCATTTGACGAATTCGAGGTCGATCGATGTGTTCTTCCCAAGGGTGCATCTGTCTCATTTCCCGCGAGCCAAGGTCCTTCGATATTTCTGGTTGCTGGAGGAGAGGGGAGGATGCTGCCTCAAGAATCACTTAGAAGTGATGTAATCAGTGAAGGAGACGTCCTCTTTGTACCTGCTAAAACTGGGATTAACATAACAAGTAATGTTGAGTTGCTCATTTTCAGAGCAGGAGAAAACGAATTACATCCACAAACAAATGCCTGCAAGCAAACTTTCAGAAGCTCAATCATGATAGAGTTTAGACAAGACCTGCATCACTTCAAGTCCTCCAAAGCATCTGAAGAAAGAGGTTTGAATATGGAAGTCATAAACCAGTTCCCAAATATGACAGAGAGCATGTTAGAGGACTTCAATGAAACAGACTTCTCAGAAGAAAACTTCTTGGGCTACCATTTCCAGTTACCAGAAAACACAACACGGAGCTCAATTTCTGATTTTCCTCGTGTTGCCCTGTTCAAATGTCAGAATGAGTTTCCGGTGATAACTCGAAGCAGTCCACTGGTAGATAGCTCTCAAGAAACCATGAATAGAAAAGCAAAACAAGTATTGAACTTAGGAGGAAGGAAAAGAAGGAAATGTGAGAAAGAACAAGAAAAGCCTGCAGAAGTAGTTCATATTAGAGCAAAAAGAGGTGAAGCTACCCATAGTCACAGCTTAGCAGAAAGGGTGAGAAGGGAGAAAATCAACAACAAATTAAAATGCTTACAAAACCTTGTTCCAGGTTGCCACAAGACAATGGGTATGACAATGGTGTTGGATGAGACAATCACTTATGTTCATTCACTACAGAATCAGGTTGAGTTTCTCTCAATGGAGCTTGCAACTGCCTGTTCCACTTTAGGCATAAATTCTGGCATGGGAGCTACCAGAAAAGCTTCGACAGAAACTATAAGGGGGCTAGCCATGGCCCTACGTACAAACGATAAGCAAAAGATCCCAAGGCCATTACCGATTAAAGAGAATAAAACTGTGGGGAATAGTCATTGGGGCATTTCAGCGAATACCTTGCATACATGGAAGTGGGGTTTCGTGGGCATTGCCATTGCTACATGTGACGAAGCAAATCAAACTCAAATCAAATTCAGATATTCTCATCCTCAGGTAGAATATTAA

Protein sequence

MESDPVLTQKQSNVRRLKCSVQNYDWGIRGHDSLVARLFALNSGSSVASHKPYAEFWMGTHDSGPSFVIAAAADNGGAIDSDSISLKSWVLENPNVLGDKVVEKWGSDIPFLFKVLSVAKPLSIQAHPDKELARLLHKVEPKVYRDANHKPEMALAITEFEALCGFISVEELKDVLRSVPEILVMIRDADRNRVLLLDDPANKEADLKAALRLAFTQLMSASKILISRAISDLKRRLHSESEVRQLTAKELLVVRLEKQYPDDVGIIAAFFLNHVKLNPGEALYLGANEPHAYISGECIECMATSDNVIRAGLTPKFRDVHTLCAMFTYKQGFPEILQGVHVNQYITKYVPPFDEFEVDRCVLPKGASVSFPASQGPSIFLVAGGEGRMLPQESLRSDVISEGDVLFVPAKTGINITSNVELLIFRAGENELHPQTNACKQTFRSSIMIEFRQDLHHFKSSKASEERGLNMEVINQFPNMTESMLEDFNETDFSEENFLGYHFQLPENTTRSSISDFPRVALFKCQNEFPVITRSSPLVDSSQETMNRKAKQVLNLGGRKRRKCEKEQEKPAEVVHIRAKRGEATHSHSLAERVRREKINNKLKCLQNLVPGCHKTMGMTMVLDETITYVHSLQNQVEFLSMELATACSTLGINSGMGATRKASTETIRGLAMALRTNDKQKIPRPLPIKENKTVGNSHWGISANTLHTWKWGFVGIAIATCDEANQTQIKFRYSHPQVEY
Homology
BLAST of CmoCh01G004000 vs. ExPASy Swiss-Prot
Match: Q9M884 (Mannose-6-phosphate isomerase 1 OS=Arabidopsis thaliana OX=3702 GN=PMI1 PE=1 SV=1)

HSP 1 Score: 495.4 bits (1274), Expect = 1.2e-138
Identity = 242/417 (58.03%), Postives = 315/417 (75.54%), Query Frame = 0

Query: 14  VRRLKCSVQNYDWGIRGHDSLVARLFALNSGSSVASHKPYAEFWMGTHDSGPSFVIAAAA 73
           +RRL+CSV++YDWG  G DSLV R++A NS   +   +PYAE WMGTH+SGPS++  A  
Sbjct: 20  LRRLRCSVKDYDWGKIGSDSLVYRVYAANSDYEIDPTRPYAELWMGTHESGPSYLEDADG 79

Query: 74  DNGGAIDSDSISLKSWVLENPNVLGDKVVEKWGSDIPFLFKVLSVAKPLSIQAHPDKELA 133
            NG       ++L+SW+ ENP  LG++V+EKWG D+PFLFKVLSVA+PLSIQAHPDK+LA
Sbjct: 80  SNG-------VTLRSWITENPKSLGNRVLEKWGCDLPFLFKVLSVARPLSIQAHPDKKLA 139

Query: 134 RLLHKVEPKVYRDANHKPEMALAITEFEALCGFISVEELKDVLRSVPEILVMIRDADRNR 193
           + +HK  P +Y+D NHKPEMALA T+FEALCGFI ++ELK V+R++PEI  ++   + N+
Sbjct: 140 KKMHKAHPNLYKDDNHKPEMALAYTQFEALCGFIPLQELKSVIRAIPEIEELVGSEEANQ 199

Query: 194 VLLLDDPANKEADLKAALRLAFTQLMSASKILISRAISDLKRRLHSESEVRQLTAKELLV 253
           V  + +  + E  +K+ +R  FT LMSA      + +S LKRRLH ES+ RQLT KE LV
Sbjct: 200 VFCITE--HDEEKVKSVVRTIFTLLMSADADTTKKIVSKLKRRLHMESQERQLTDKERLV 259

Query: 254 VRLEKQYPDDVGIIAAFFLNHVKLNPGEALYLGANEPHAYISGECIECMATSDNVIRAGL 313
           ++LEKQYP+D+G+I+AFF N+VKLNPGEALYLGANEPHAY+ GEC+E MATSDNV+RAGL
Sbjct: 260 LKLEKQYPNDIGVISAFFFNYVKLNPGEALYLGANEPHAYLFGECLEVMATSDNVVRAGL 319

Query: 314 TPKFRDVHTLCAMFTYKQGFPEILQGVHVNQYITKYVPPFDEFEVDRCVLPKGASVSFPA 373
           T K  D+ TLC+M +YK G+PEIL+G  +  YIT+Y+PPF+EFEVD C LP GAS  FP+
Sbjct: 320 TSKPLDIQTLCSMLSYKLGYPEILKGTRIRPYITRYLPPFEEFEVDLCDLPSGASTVFPS 379

Query: 374 SQGPSIFLVAGGEGRMLPQESLRSDVISEGDVLFVPAKTGINITSNVELLIFRAGEN 431
             GPS+ LV  GEGRM  + S  +D IS GDVLFVPA T I++ S+ +L ++RAG N
Sbjct: 380 VPGPSLLLVLQGEGRMSTEAS--ADGISMGDVLFVPADTEIHLRSSSDLKLYRAGIN 425

BLAST of CmoCh01G004000 vs. ExPASy Swiss-Prot
Match: Q9FZH5 (Mannose-6-phosphate isomerase 2 OS=Arabidopsis thaliana OX=3702 GN=PMI2 PE=1 SV=1)

HSP 1 Score: 471.1 bits (1211), Expect = 2.3e-131
Identity = 233/424 (54.95%), Postives = 313/424 (73.82%), Query Frame = 0

Query: 14  VRRLKCSVQNYDWGIRGHDSLVARLFALNSGSSVASHKPYAEFWMGTHDSGPSFVIAAAA 73
           ++RL+C V+NY+WG  G +SLVARL   N+G  V S  PYAEFWMGTH+SGPS V     
Sbjct: 22  IQRLRCFVKNYEWGKLGPESLVARLQEANTGQRVDSEIPYAEFWMGTHESGPSHV--EFG 81

Query: 74  DNGGAIDSDSISLKSWVLENPNVLGDKVVEKWGSDIPFLFKVLSVAKPLSIQAHPDKELA 133
              G  D   ++LKSWVL+NPN+LG KVV+KWG D+PFLFKVLSV K LSIQAHP+K LA
Sbjct: 82  SGHGVSDKCMVTLKSWVLDNPNLLGSKVVDKWGCDLPFLFKVLSVTKALSIQAHPNKALA 141

Query: 134 RLLHKVEPKVYRDANHKPEMALAITEFEALCGFISVEELKDVLRSVPEILVMIRDADRNR 193
             LH+ +P +YRD NHKPE+ALA+T F+ALCGF++++ELK+V+ +VPEI  ++     ++
Sbjct: 142 EKLHREDPLLYRDNNHKPEIALAVTPFQALCGFVTLKELKEVITNVPEITELVGSKAADQ 201

Query: 194 VLLLDDPANKEADLKAALRLAFTQLMSASKILISRAISDLKRRLHSESEVRQLTAKELLV 253
           +  + +  +++  +K+ +RL FTQLMSAS     + +S +K RL  E++ R+L+ KE LV
Sbjct: 202 IFNVHE-HDEDERIKSVVRLIFTQLMSASNNETKQVVSRMKNRLLLETKHRELSEKEKLV 261

Query: 254 VRLEKQYPDDVGIIAAFFLNHVKLNPGEALYLGANEPHAYISGECIECMATSDNVIRAGL 313
           + LEKQY  D+G+I+AFF N+VKLNPGEALYL ANEPHAYISG+C+ECMA SDNV+RAGL
Sbjct: 262 LELEKQYTGDIGVISAFFFNYVKLNPGEALYLDANEPHAYISGDCVECMAASDNVVRAGL 321

Query: 314 TPKFRDVHTLCAMFTYKQGFPEILQGVHVNQYITKYVPPFDEFEVDRCVLPKGASVSFPA 373
           TPK RDV TLC+M TYK G+PEIL+G  +  Y+T+Y+PPFDEFEVD C LP+G S  FPA
Sbjct: 322 TPKHRDVQTLCSMLTYKLGYPEILKGFPLTPYVTRYLPPFDEFEVDHCDLPRGKSTVFPA 381

Query: 374 SQGPSIFLVAGGEGRMLPQESLRSDVISEGDVLFVPAKTGINITSNVELL-IFRAGENEL 433
             GPS++LV  G+G++    S    +++ GDVLFVPA   I++T   +++ ++RAG +  
Sbjct: 382 VPGPSVYLVIEGKGQLRTGSS--KVLVNRGDVLFVPADIEIHVTGESDVMKLYRAGVSSR 440

Query: 434 HPQT 437
             QT
Sbjct: 442 FFQT 440

BLAST of CmoCh01G004000 vs. ExPASy Swiss-Prot
Match: Q8HXX2 (Mannose-6-phosphate isomerase OS=Macaca fascicularis OX=9541 GN=MPI PE=2 SV=3)

HSP 1 Score: 283.1 bits (723), Expect = 9.0e-75
Identity = 167/423 (39.48%), Postives = 240/423 (56.74%), Query Frame = 0

Query: 17  LKCSVQNYDWGIRGHDSLVARLFALNSG-SSVASHKPYAEFWMGTHDSGPSFVIAAAADN 76
           L C+VQ Y WG  G++S VARL A +   + +A  KPYAE WMGTH  G + +     DN
Sbjct: 9   LSCAVQQYAWGKMGYNSEVARLLASSDPLAQIAEDKPYAELWMGTHPRGDAKIF----DN 68

Query: 77  GGAIDSDSISLKSWVLENPNVLGDKVVEKWGSDIPFLFKVLSVAKPLSIQAHPDKELARL 136
           G        +L  W+ EN + LG KV + +  ++PFLFKVLSV  PLSIQAHP+KELA  
Sbjct: 69  G----ISQKTLGQWIAENQDSLGSKVKDTFNGNLPFLFKVLSVETPLSIQAHPNKELAEK 128

Query: 137 LHKVEPKVYRDANHKPEMALAITEFEALCGFISVEELKDVLRSVPEILVMIRDADRNRVL 196
           LH   P+ Y DANHKPEMA+A+T F+ LCGF  VEE+   L+ VPE   +I   D     
Sbjct: 129 LHLQAPQHYPDANHKPEMAIALTPFQGLCGFRPVEEIVTFLKKVPEFQFLI--GDEAATH 188

Query: 197 LLDDPANKEADLKAALRLAFTQLMSASKILISRAISDLKRRLHSE----SEVRQLTAKEL 256
           L    ++    + +AL+  F+ LM + K +++  ++ L +R+  +    + +  +  +  
Sbjct: 189 LKQTMSHDSQAVASALQSCFSHLMKSEKKVVAEQLNLLVKRISQQVAAGNNMEDIFGE-- 248

Query: 257 LVVRLEKQYPDDVGIIAAFFLNHVKLNPGEALYLGANEPHAYISGECIECMATSDNVIRA 316
           L+++L +QYP D+G  A +FLN + L PGEA++L AN PHAY+ G+C+ECMA SDN +RA
Sbjct: 249 LLLQLHQQYPGDIGCFAIYFLNLLNLKPGEAMFLEANVPHAYLKGDCVECMACSDNTVRA 308

Query: 317 GLTPKFRDVHTLCAMFTYKQGFPE----ILQGVHVNQYITKYVPPFDEFEVDRCVLPKGA 376
           GLTPKF DV TLC M +Y     +    +      + Y++ Y PP  +F + +  +P   
Sbjct: 309 GLTPKFIDVPTLCEMLSYTPSSSKDRLFLPTRSQEDPYLSIYDPPVPDFAIMKMEVPGSV 368

Query: 377 S-VSFPASQGPSIFLVAGGEGRMLPQESLRSDVISEGDVLFVPAKTGIN--ITSNVELLI 428
           +     A    SI LV  G        +     +  G VLF+ A   ++  +T   +LLI
Sbjct: 369 TEYKVLALDSASILLVVQGTVIASTPTTQTPIPLQRGGVLFIGANESVSLKLTEPKDLLI 419

BLAST of CmoCh01G004000 vs. ExPASy Swiss-Prot
Match: Q54PA0 (Probable mannose-6-phosphate isomerase OS=Dictyostelium discoideum OX=44689 GN=mpi PE=3 SV=1)

HSP 1 Score: 277.7 bits (709), Expect = 3.8e-73
Identity = 169/420 (40.24%), Postives = 245/420 (58.33%), Query Frame = 0

Query: 12  SNVRRLKCSVQNYDWGIRGHDSLVARL---FALNSGSSVASHKPYAEFWMGTHDSGPSFV 71
           +N+  LKC  QNY+WG  G +S VA+L   +A      +    PYAE WMG H S PS V
Sbjct: 19  NNIVLLKCVSQNYEWGKYGSNSTVAKLLKGYAKECSDIIKETIPYAELWMGDHVSAPSKV 78

Query: 72  --------IAAAADNGGAIDSDSISLKSWVLENPNVLGDKVVEKWGSDIPFLFKVLSVAK 131
                   +    D      ++  S  S    + ++ G+ V +++G+D PFLFKVLS+  
Sbjct: 79  EYKNKELKLREYIDTVQKEINEKSSTSS--SSSSSIRGEIVEKRFGNDFPFLFKVLSIRT 138

Query: 132 PLSIQAHPDKELARLLHKVEPKVYRDANHKPEMALAITEFEALCGFISVEELKDVLRSVP 191
            LSIQAHPD +LA++L K  P +Y+D  HKPE+A+A T FEALC F  + E++  + ++P
Sbjct: 139 ALSIQAHPDSQLAQVLFKKYPNIYKDPYHKPEIAIATTPFEALCSFRPLSEIQSFIDTIP 198

Query: 192 EILVMIRDADRNRVLLLDDPANKEADLKAALRLAFTQLMSASKILISRAISDLKRRLHSE 251
           E     +++  N ++ LD       D K  L+   T L+ A  +LIS  + +L  RL+ +
Sbjct: 199 E----FKNSLPNNLIKLD-------DCKEYLKSIVTSLLKADGLLISNNLKELNNRLNEK 258

Query: 252 SEVRQLTAKELLVVRLEKQYPDDVGIIAAFFLNHVKLNPGEALYLGANEPHAYISGECIE 311
            E  +    + LV++L  QYP DVG+  A+ LN++ L PGEAL+LGA EPHAYISG+C+E
Sbjct: 259 RE-EERDDLDRLVLKLYSQYPGDVGVFFAYILNYIVLKPGEALFLGAGEPHAYISGDCVE 318

Query: 312 CMATSDNVIRAGLTPKFRDVHTLCAMFTYKQGFPEIL-----QGVHVNQYITKYVPPFDE 371
           CMA SDNV+RAGLTPK +DV TL  M TY+ G P+++       + +  Y   Y PP DE
Sbjct: 319 CMAPSDNVVRAGLTPKLKDVDTLGDMLTYRTGRPDLVVPQQRPDLSLTNY-RCYQPPVDE 378

Query: 372 FEVDRCVLPKGA-SVSFPASQGPSIFLVAGGEGRMLPQESLRSDV--ISEGDVLFVPAKT 413
           F+V+   L     +++  +S+GPSI LV  G    +  +S +S +  +  G +LFVPA T
Sbjct: 379 FQVEYYHLDDCCKNINVYSSKGPSIVLVYSG-NLSIENKSNQSTLNNLHTGSILFVPANT 422

BLAST of CmoCh01G004000 vs. ExPASy Swiss-Prot
Match: P34949 (Mannose-6-phosphate isomerase OS=Homo sapiens OX=9606 GN=MPI PE=1 SV=2)

HSP 1 Score: 276.9 bits (707), Expect = 6.5e-73
Identity = 164/421 (38.95%), Postives = 235/421 (55.82%), Query Frame = 0

Query: 17  LKCSVQNYDWGIRGHDSLVARLFALNSG-SSVASHKPYAEFWMGTHDSGPSFVIAAAADN 76
           L C+VQ Y WG  G +S VARL A +   + +A  KPYAE WMGTH  G + ++    DN
Sbjct: 9   LSCAVQQYAWGKMGSNSEVARLLASSDPLAQIAEDKPYAELWMGTHPRGDAKIL----DN 68

Query: 77  GGAIDSDSISLKSWVLENPNVLGDKVVEKWGSDIPFLFKVLSVAKPLSIQAHPDKELARL 136
                    +L  W+ EN + LG KV + +  ++PFLFKVLSV  PLSIQAHP+KELA  
Sbjct: 69  ----RISQKTLSQWIAENQDSLGSKVKDTFNGNLPFLFKVLSVETPLSIQAHPNKELAEK 128

Query: 137 LHKVEPKVYRDANHKPEMALAITEFEALCGFISVEELKDVLRSVPEILVMIRDADRNRVL 196
           LH   P+ Y DANHKPEMA+A+T F+ LCGF  VEE+   L+ VPE   +I   D     
Sbjct: 129 LHLQAPQHYPDANHKPEMAIALTPFQGLCGFRPVEEIVTFLKKVPEFQFLI--GDEAATH 188

Query: 197 LLDDPANKEADLKAALRLAFTQLMSASKILISRAISDLKRRLHSESEVRQLTAKEL--LV 256
           L    ++    + ++L+  F+ LM + K ++   ++ L +R+  ++            L+
Sbjct: 189 LKQTMSHDSQAVASSLQSCFSHLMKSEKKVVVEQLNLLVKRISQQAAAGNNMEDIFGELL 248

Query: 257 VRLEKQYPDDVGIIAAFFLNHVKLNPGEALYLGANEPHAYISGECIECMATSDNVIRAGL 316
           ++L +QYP D+G  A +FLN + L PGEA++L AN PHAY+ G+C+ECMA SDN +RAGL
Sbjct: 249 LQLHQQYPGDIGCFAIYFLNLLTLKPGEAMFLEANVPHAYLKGDCVECMACSDNTVRAGL 308

Query: 317 TPKFRDVHTLCAMFTYKQGFPE----ILQGVHVNQYITKYVPPFDEFEVDRCVLPKGAS- 376
           TPKF DV TLC M +Y     +    +      + Y++ Y PP  +F + +  +P   + 
Sbjct: 309 TPKFIDVPTLCEMLSYTPSSSKDRLFLPTRSQEDPYLSIYDPPVPDFTIMKTEVPGSVTE 368

Query: 377 VSFPASQGPSIFLVAGGEGRMLPQESLRSDVISEGDVLFVPAKTGIN--ITSNVELLIFR 428
               A    SI L+  G        +     +  G VLF+ A   ++  +T   +LLIFR
Sbjct: 369 YKVLALDSASILLMVQGTVIASTPTTQTPIPLQRGGVLFIGANESVSLKLTEPKDLLIFR 419

BLAST of CmoCh01G004000 vs. ExPASy TrEMBL
Match: A0A6J1G9H7 (Mannose-6-phosphate isomerase OS=Cucurbita moschata OX=3662 GN=LOC111452034 PE=3 SV=1)

HSP 1 Score: 850.1 bits (2195), Expect = 6.8e-243
Identity = 429/430 (99.77%), Postives = 429/430 (99.77%), Query Frame = 0

Query: 1   MESDPVLTQKQSNVRRLKCSVQNYDWGIRGHDSLVARLFALNSGSSVASHKPYAEFWMGT 60
           MESDPVLTQKQSNVRRLKCSVQNYDWGIRGHDSLVARLFALNSGSSVASHKPYAEFWMGT
Sbjct: 1   MESDPVLTQKQSNVRRLKCSVQNYDWGIRGHDSLVARLFALNSGSSVASHKPYAEFWMGT 60

Query: 61  HDSGPSFVIAAAADNGGAIDSDSISLKSWVLENPNVLGDKVVEKWGSDIPFLFKVLSVAK 120
           HDSGPSFVIAAAADNGGAIDSDSISLKSWVLENPNVLGDKVVEKWGSDIPFLFKVLSVAK
Sbjct: 61  HDSGPSFVIAAAADNGGAIDSDSISLKSWVLENPNVLGDKVVEKWGSDIPFLFKVLSVAK 120

Query: 121 PLSIQAHPDKELARLLHKVEPKVYRDANHKPEMALAITEFEALCGFISVEELKDVLRSVP 180
           PLSIQAHPDKELARLLHKVEPKVYRDANHKPEMALAITEFEALCGFISVEELKDVLRSVP
Sbjct: 121 PLSIQAHPDKELARLLHKVEPKVYRDANHKPEMALAITEFEALCGFISVEELKDVLRSVP 180

Query: 181 EILVMIRDADRNRVLLLDDPANKEADLKAALRLAFTQLMSASKILISRAISDLKRRLHSE 240
           EILVMIRDADRNRVLLLDDPANKEADLKAALRLAFTQLMSASKILISRAISDLKRRLHSE
Sbjct: 181 EILVMIRDADRNRVLLLDDPANKEADLKAALRLAFTQLMSASKILISRAISDLKRRLHSE 240

Query: 241 SEVRQLTAKELLVVRLEKQYPDDVGIIAAFFLNHVKLNPGEALYLGANEPHAYISGECIE 300
           SEVRQLTAKELLVVRLEKQYPDDVGIIAAFFLNHVKLNPGEALYLGANEPHAYISGECIE
Sbjct: 241 SEVRQLTAKELLVVRLEKQYPDDVGIIAAFFLNHVKLNPGEALYLGANEPHAYISGECIE 300

Query: 301 CMATSDNVIRAGLTPKFRDVHTLCAMFTYKQGFPEILQGVHVNQYITKYVPPFDEFEVDR 360
           CMATSDNVIRAGLTPKFRDVHTLCAMFTYKQGFPEILQGVHVNQYITKYVPPFDEFEVDR
Sbjct: 301 CMATSDNVIRAGLTPKFRDVHTLCAMFTYKQGFPEILQGVHVNQYITKYVPPFDEFEVDR 360

Query: 361 CVLPKGASVSFPASQGPSIFLVAGGEGRMLPQESLRSDVISEGDVLFVPAKTGINITSNV 420
           CVLPKGASVSFPASQGPSIFLVAGGEGRMLPQESLRSDVISEGDVLFVPAKTGINITSNV
Sbjct: 361 CVLPKGASVSFPASQGPSIFLVAGGEGRMLPQESLRSDVISEGDVLFVPAKTGINITSNV 420

Query: 421 ELLIFRAGEN 431
           ELLIFRAG N
Sbjct: 421 ELLIFRAGVN 430

BLAST of CmoCh01G004000 vs. ExPASy TrEMBL
Match: A0A6J1K5R1 (Mannose-6-phosphate isomerase OS=Cucurbita maxima OX=3661 GN=LOC111492565 PE=3 SV=1)

HSP 1 Score: 816.2 bits (2107), Expect = 1.1e-232
Identity = 411/430 (95.58%), Postives = 420/430 (97.67%), Query Frame = 0

Query: 1   MESDPVLTQKQSNVRRLKCSVQNYDWGIRGHDSLVARLFALNSGSSVASHKPYAEFWMGT 60
           MESDPVLT KQSNVRRLKCSVQNYDWGIRGHDSLVARLFALNSGSSVASHKPYAEFWMGT
Sbjct: 1   MESDPVLTPKQSNVRRLKCSVQNYDWGIRGHDSLVARLFALNSGSSVASHKPYAEFWMGT 60

Query: 61  HDSGPSFVIAAAADNGGAIDSDSISLKSWVLENPNVLGDKVVEKWGSDIPFLFKVLSVAK 120
           HDSGPSFVI AAADNGGAIDSDS SLKSWV ENPNVLGDKV EKWGSDIPFLFKVLSVAK
Sbjct: 61  HDSGPSFVI-AAADNGGAIDSDSTSLKSWVSENPNVLGDKVAEKWGSDIPFLFKVLSVAK 120

Query: 121 PLSIQAHPDKELARLLHKVEPKVYRDANHKPEMALAITEFEALCGFISVEELKDVLRSVP 180
           PLSIQAHPDKELA+LLHKVEPKVYRDANHKPEMALAITEFEALCGFISVEELKDVLRSVP
Sbjct: 121 PLSIQAHPDKELAKLLHKVEPKVYRDANHKPEMALAITEFEALCGFISVEELKDVLRSVP 180

Query: 181 EILVMIRDADRNRVLLLDDPANKEADLKAALRLAFTQLMSASKILISRAISDLKRRLHSE 240
           EIL+MIRDADRNR+LLLDD ANKE DLKAALRLAFTQLMSASKILISRAISDLKRRLHSE
Sbjct: 181 EILLMIRDADRNRLLLLDDHANKEEDLKAALRLAFTQLMSASKILISRAISDLKRRLHSE 240

Query: 241 SEVRQLTAKELLVVRLEKQYPDDVGIIAAFFLNHVKLNPGEALYLGANEPHAYISGECIE 300
           SEVRQLTAKELLV+RLEKQYPDDVGIIAAFFLNHVKLNPGEALYLGANEPHAYISGECIE
Sbjct: 241 SEVRQLTAKELLVLRLEKQYPDDVGIIAAFFLNHVKLNPGEALYLGANEPHAYISGECIE 300

Query: 301 CMATSDNVIRAGLTPKFRDVHTLCAMFTYKQGFPEILQGVHVNQYITKYVPPFDEFEVDR 360
           CMATSDNV+RAGLTPKFRDVHTLCA+FTYKQGFPEILQGVHVNQYITKYVPPF+EFEVDR
Sbjct: 301 CMATSDNVVRAGLTPKFRDVHTLCAIFTYKQGFPEILQGVHVNQYITKYVPPFEEFEVDR 360

Query: 361 CVLPKGASVSFPASQGPSIFLVAGGEGRMLPQESLRSDVISEGDVLFVPAKTGINITSNV 420
           CVLPKGASV FP SQGPSIFLVAGGEGRMLPQ+SLR+DVISEGDVLFVPAKTGINITSNV
Sbjct: 361 CVLPKGASVLFPVSQGPSIFLVAGGEGRMLPQDSLRNDVISEGDVLFVPAKTGINITSNV 420

Query: 421 ELLIFRAGEN 431
           ELLIFRAG N
Sbjct: 421 ELLIFRAGVN 429

BLAST of CmoCh01G004000 vs. ExPASy TrEMBL
Match: A0A6J1KEL2 (Mannose-6-phosphate isomerase OS=Cucurbita maxima OX=3661 GN=LOC111492565 PE=3 SV=1)

HSP 1 Score: 781.2 bits (2016), Expect = 3.9e-222
Identity = 392/411 (95.38%), Postives = 402/411 (97.81%), Query Frame = 0

Query: 1   MESDPVLTQKQSNVRRLKCSVQNYDWGIRGHDSLVARLFALNSGSSVASHKPYAEFWMGT 60
           MESDPVLT KQSNVRRLKCSVQNYDWGIRGHDSLVARLFALNSGSSVASHKPYAEFWMGT
Sbjct: 1   MESDPVLTPKQSNVRRLKCSVQNYDWGIRGHDSLVARLFALNSGSSVASHKPYAEFWMGT 60

Query: 61  HDSGPSFVIAAAADNGGAIDSDSISLKSWVLENPNVLGDKVVEKWGSDIPFLFKVLSVAK 120
           HDSGPSFVI AAADNGGAIDSDS SLKSWV ENPNVLGDKV EKWGSDIPFLFKVLSVAK
Sbjct: 61  HDSGPSFVI-AAADNGGAIDSDSTSLKSWVSENPNVLGDKVAEKWGSDIPFLFKVLSVAK 120

Query: 121 PLSIQAHPDKELARLLHKVEPKVYRDANHKPEMALAITEFEALCGFISVEELKDVLRSVP 180
           PLSIQAHPDKELA+LLHKVEPKVYRDANHKPEMALAITEFEALCGFISVEELKDVLRSVP
Sbjct: 121 PLSIQAHPDKELAKLLHKVEPKVYRDANHKPEMALAITEFEALCGFISVEELKDVLRSVP 180

Query: 181 EILVMIRDADRNRVLLLDDPANKEADLKAALRLAFTQLMSASKILISRAISDLKRRLHSE 240
           EIL+MIRDADRNR+LLLDD ANKE DLKAALRLAFTQLMSASKILISRAISDLKRRLHSE
Sbjct: 181 EILLMIRDADRNRLLLLDDHANKEEDLKAALRLAFTQLMSASKILISRAISDLKRRLHSE 240

Query: 241 SEVRQLTAKELLVVRLEKQYPDDVGIIAAFFLNHVKLNPGEALYLGANEPHAYISGECIE 300
           SEVRQLTAKELLV+RLEKQYPDDVGIIAAFFLNHVKLNPGEALYLGANEPHAYISGECIE
Sbjct: 241 SEVRQLTAKELLVLRLEKQYPDDVGIIAAFFLNHVKLNPGEALYLGANEPHAYISGECIE 300

Query: 301 CMATSDNVIRAGLTPKFRDVHTLCAMFTYKQGFPEILQGVHVNQYITKYVPPFDEFEVDR 360
           CMATSDNV+RAGLTPKFRDVHTLCA+FTYKQGFPEILQGVHVNQYITKYVPPF+EFEVDR
Sbjct: 301 CMATSDNVVRAGLTPKFRDVHTLCAIFTYKQGFPEILQGVHVNQYITKYVPPFEEFEVDR 360

Query: 361 CVLPKGASVSFPASQGPSIFLVAGGEGRMLPQESLRSDVISEGDVLFVPAK 412
           CVLPKGASV FP SQGPSIFLVAGGEGRMLPQ+SLR+DVISEGDVLFVPA+
Sbjct: 361 CVLPKGASVLFPVSQGPSIFLVAGGEGRMLPQDSLRNDVISEGDVLFVPAE 410

BLAST of CmoCh01G004000 vs. ExPASy TrEMBL
Match: A0A6J1CQ99 (Mannose-6-phosphate isomerase OS=Momordica charantia OX=3673 GN=LOC111013172 PE=3 SV=1)

HSP 1 Score: 696.4 bits (1796), Expect = 1.3e-196
Identity = 357/430 (83.02%), Postives = 382/430 (88.84%), Query Frame = 0

Query: 1   MESDPVLTQKQSNVRRLKCSVQNYDWGIRGHDSLVARLFALNSGSSVASHKPYAEFWMGT 60
           MES+ VL  KQSNV RLKCSVQNYDWGIRG DS VARLFALNSGS V S KPYAEFWMGT
Sbjct: 1   MESEAVLRTKQSNVFRLKCSVQNYDWGIRGPDSAVARLFALNSGSIVDSDKPYAEFWMGT 60

Query: 61  HDSGPSFVIAAAADNGGAIDSDSISLKSWVLENPNVLGDKVVEKWGSDIPFLFKVLSVAK 120
           HDSGPSF+I  AA+NGGA++SDS SLKSWV ENPN LG+KVVEKWGSD+PFLFKVLSVAK
Sbjct: 61  HDSGPSFLI-GAAENGGAVESDSTSLKSWVSENPNSLGEKVVEKWGSDLPFLFKVLSVAK 120

Query: 121 PLSIQAHPDKELARLLHKVEPKVYRDANHKPEMALAITEFEALCGFISVEELKDVLRSVP 180
            LSIQAHPDKELA++LHK++PK YRDANHKPEMALAITEFEALCGFIS+EELKDVLRSVP
Sbjct: 121 ALSIQAHPDKELAKILHKLQPKAYRDANHKPEMALAITEFEALCGFISLEELKDVLRSVP 180

Query: 181 EILVMIRDADRNRVLLLDDPANKEADLKAALRLAFTQLMSASKILISRAISDLKRRLHSE 240
           E + MI   D NRVLLLDD  ++E DLK+ LR AFTQLMSA+K +IS  ISDLKRRLHSE
Sbjct: 181 ETVEMIGHEDTNRVLLLDD-EDEEEDLKSVLRSAFTQLMSATKSMISGTISDLKRRLHSE 240

Query: 241 SEVRQLTAKELLVVRLEKQYPDDVGIIAAFFLNHVKLNPGEALYLGANEPHAYISGECIE 300
           SEVRQLTAKELLV+RLEKQYPDDVG+IAAFFLN+VKLNPGEALYLGANEPHAYISGECIE
Sbjct: 241 SEVRQLTAKELLVLRLEKQYPDDVGVIAAFFLNYVKLNPGEALYLGANEPHAYISGECIE 300

Query: 301 CMATSDNVIRAGLTPKFRDVHTLCAMFTYKQGFPEILQGVHVNQYITKYVPPFDEFEVDR 360
           CMATSDNV+RAGLTPKFRDV TLCAM TYK GFPEILQGV VN YITKY+PPFDEFEVD 
Sbjct: 301 CMATSDNVVRAGLTPKFRDVQTLCAMLTYKLGFPEILQGVPVNPYITKYIPPFDEFEVDC 360

Query: 361 CVLPKGASVSFPASQGPSIFLVAGGEGRMLPQESLRSDVISEGDVLFVPAKTGINITSNV 420
           CVLP+GASV FPAS GPSI LVA GEG MLP ESL SDVISEGDVLFV AKT I+ITS V
Sbjct: 361 CVLPEGASVLFPASPGPSICLVARGEGMMLPLESLESDVISEGDVLFVAAKTEISITSEV 420

Query: 421 ELLIFRAGEN 431
           ELLIFR G N
Sbjct: 421 ELLIFRTGVN 428

BLAST of CmoCh01G004000 vs. ExPASy TrEMBL
Match: A0A6J1J7Z8 (Mannose-6-phosphate isomerase OS=Cucurbita maxima OX=3661 GN=LOC111482189 PE=3 SV=1)

HSP 1 Score: 694.9 bits (1792), Expect = 3.7e-196
Identity = 354/430 (82.33%), Postives = 386/430 (89.77%), Query Frame = 0

Query: 1   MESDPVLTQKQSNVRRLKCSVQNYDWGIRGHDSLVARLFALNSGSSVASHKPYAEFWMGT 60
           MESD V   KQSN+ RLKCSV+NYDWGIRG+DS VARLFALNSGS +   KPYAEFWMGT
Sbjct: 1   MESDAVSRMKQSNIFRLKCSVRNYDWGIRGYDSRVARLFALNSGSIIDPDKPYAEFWMGT 60

Query: 61  HDSGPSFVIAAAADNGGAIDSDSISLKSWVLENPNVLGDKVVEKWGSDIPFLFKVLSVAK 120
           HDSGPSF+I AA +NGG++DSDS SLKSWVLENPNVLG KVVEKWG+DIPFLFKVLSVAK
Sbjct: 61  HDSGPSFLI-AATENGGSVDSDSTSLKSWVLENPNVLGYKVVEKWGADIPFLFKVLSVAK 120

Query: 121 PLSIQAHPDKELARLLHKVEPKVYRDANHKPEMALAITEFEALCGFISVEELKDVLRSVP 180
           PLSIQAHPDKELA+ LH ++PKVYRDANHKPEMALAITEFEALCGFIS+EELKDVLR+VP
Sbjct: 121 PLSIQAHPDKELAKELHMLQPKVYRDANHKPEMALAITEFEALCGFISLEELKDVLRTVP 180

Query: 181 EILVMIRDADRNRVLLLDDPANKEADLKAALRLAFTQLMSASKILISRAISDLKRRLHSE 240
           EI+ MI DAD NRVLLL+D  + E +LK+ALR AFTQLMSASK LIS+AIS+LKRRL+SE
Sbjct: 181 EIVEMIGDADTNRVLLLND-EDDEDNLKSALRSAFTQLMSASKSLISKAISELKRRLNSE 240

Query: 241 SEVRQLTAKELLVVRLEKQYPDDVGIIAAFFLNHVKLNPGEALYLGANEPHAYISGECIE 300
           SEVR LTAKE LV++LEKQYPDDVG+IAAFFLN+VKL PGEAL+LGANEPHAYISGECIE
Sbjct: 241 SEVRPLTAKEALVLQLEKQYPDDVGVIAAFFLNYVKLTPGEALHLGANEPHAYISGECIE 300

Query: 301 CMATSDNVIRAGLTPKFRDVHTLCAMFTYKQGFPEILQGVHVNQYITKYVPPFDEFEVDR 360
           CMATSDNVIRAGLTPKFRDV TLCAM TYKQGFPEILQGV +N YITKYVPPFDEFEVDR
Sbjct: 301 CMATSDNVIRAGLTPKFRDVQTLCAMLTYKQGFPEILQGVPMNPYITKYVPPFDEFEVDR 360

Query: 361 CVLPKGASVSFPASQGPSIFLVAGGEGRMLPQESLRSDVISEGDVLFVPAKTGINITSNV 420
           CVLP GASV FPAS GPSIFLVAGGEG ML +ES RSDVISEGDVLF+P +  +NITS V
Sbjct: 361 CVLPGGASVVFPASPGPSIFLVAGGEGTMLHEESHRSDVISEGDVLFIPDEAEVNITSEV 420

Query: 421 ELLIFRAGEN 431
           ELLIFR G N
Sbjct: 421 ELLIFRTGVN 428

BLAST of CmoCh01G004000 vs. NCBI nr
Match: XP_022948320.1 (mannose-6-phosphate isomerase 1-like [Cucurbita moschata])

HSP 1 Score: 850.1 bits (2195), Expect = 1.4e-242
Identity = 429/430 (99.77%), Postives = 429/430 (99.77%), Query Frame = 0

Query: 1   MESDPVLTQKQSNVRRLKCSVQNYDWGIRGHDSLVARLFALNSGSSVASHKPYAEFWMGT 60
           MESDPVLTQKQSNVRRLKCSVQNYDWGIRGHDSLVARLFALNSGSSVASHKPYAEFWMGT
Sbjct: 1   MESDPVLTQKQSNVRRLKCSVQNYDWGIRGHDSLVARLFALNSGSSVASHKPYAEFWMGT 60

Query: 61  HDSGPSFVIAAAADNGGAIDSDSISLKSWVLENPNVLGDKVVEKWGSDIPFLFKVLSVAK 120
           HDSGPSFVIAAAADNGGAIDSDSISLKSWVLENPNVLGDKVVEKWGSDIPFLFKVLSVAK
Sbjct: 61  HDSGPSFVIAAAADNGGAIDSDSISLKSWVLENPNVLGDKVVEKWGSDIPFLFKVLSVAK 120

Query: 121 PLSIQAHPDKELARLLHKVEPKVYRDANHKPEMALAITEFEALCGFISVEELKDVLRSVP 180
           PLSIQAHPDKELARLLHKVEPKVYRDANHKPEMALAITEFEALCGFISVEELKDVLRSVP
Sbjct: 121 PLSIQAHPDKELARLLHKVEPKVYRDANHKPEMALAITEFEALCGFISVEELKDVLRSVP 180

Query: 181 EILVMIRDADRNRVLLLDDPANKEADLKAALRLAFTQLMSASKILISRAISDLKRRLHSE 240
           EILVMIRDADRNRVLLLDDPANKEADLKAALRLAFTQLMSASKILISRAISDLKRRLHSE
Sbjct: 181 EILVMIRDADRNRVLLLDDPANKEADLKAALRLAFTQLMSASKILISRAISDLKRRLHSE 240

Query: 241 SEVRQLTAKELLVVRLEKQYPDDVGIIAAFFLNHVKLNPGEALYLGANEPHAYISGECIE 300
           SEVRQLTAKELLVVRLEKQYPDDVGIIAAFFLNHVKLNPGEALYLGANEPHAYISGECIE
Sbjct: 241 SEVRQLTAKELLVVRLEKQYPDDVGIIAAFFLNHVKLNPGEALYLGANEPHAYISGECIE 300

Query: 301 CMATSDNVIRAGLTPKFRDVHTLCAMFTYKQGFPEILQGVHVNQYITKYVPPFDEFEVDR 360
           CMATSDNVIRAGLTPKFRDVHTLCAMFTYKQGFPEILQGVHVNQYITKYVPPFDEFEVDR
Sbjct: 301 CMATSDNVIRAGLTPKFRDVHTLCAMFTYKQGFPEILQGVHVNQYITKYVPPFDEFEVDR 360

Query: 361 CVLPKGASVSFPASQGPSIFLVAGGEGRMLPQESLRSDVISEGDVLFVPAKTGINITSNV 420
           CVLPKGASVSFPASQGPSIFLVAGGEGRMLPQESLRSDVISEGDVLFVPAKTGINITSNV
Sbjct: 361 CVLPKGASVSFPASQGPSIFLVAGGEGRMLPQESLRSDVISEGDVLFVPAKTGINITSNV 420

Query: 421 ELLIFRAGEN 431
           ELLIFRAG N
Sbjct: 421 ELLIFRAGVN 430

BLAST of CmoCh01G004000 vs. NCBI nr
Match: KAG6607030.1 (Mannose-6-phosphate isomerase 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 832.4 bits (2149), Expect = 3.0e-237
Identity = 421/430 (97.91%), Postives = 425/430 (98.84%), Query Frame = 0

Query: 1   MESDPVLTQKQSNVRRLKCSVQNYDWGIRGHDSLVARLFALNSGSSVASHKPYAEFWMGT 60
           MESDPVLTQKQSNVRRLKCSVQNYDWGIRGHDSLVARLFALNSGSSVASHKPYAEFWMGT
Sbjct: 1   MESDPVLTQKQSNVRRLKCSVQNYDWGIRGHDSLVARLFALNSGSSVASHKPYAEFWMGT 60

Query: 61  HDSGPSFVIAAAADNGGAIDSDSISLKSWVLENPNVLGDKVVEKWGSDIPFLFKVLSVAK 120
           HDSGPSFVI AAADNGGAIDSDS+SLKSWVLENPNVLGDKVVEKWGSDIPFLFKVLSVAK
Sbjct: 61  HDSGPSFVI-AAADNGGAIDSDSMSLKSWVLENPNVLGDKVVEKWGSDIPFLFKVLSVAK 120

Query: 121 PLSIQAHPDKELARLLHKVEPKVYRDANHKPEMALAITEFEALCGFISVEELKDVLRSVP 180
           PLSIQAHPDKELA+LLHKVEPKVYRDANHKPEMALAITEFEALCGFISVEEL DVLRSVP
Sbjct: 121 PLSIQAHPDKELAKLLHKVEPKVYRDANHKPEMALAITEFEALCGFISVEELMDVLRSVP 180

Query: 181 EILVMIRDADRNRVLLLDDPANKEADLKAALRLAFTQLMSASKILISRAISDLKRRLHSE 240
           EILVMIRDADRNRVLLLDD ANKE DLKAALRLAFTQLMSASKILISRAISDLKRRLHSE
Sbjct: 181 EILVMIRDADRNRVLLLDDHANKEEDLKAALRLAFTQLMSASKILISRAISDLKRRLHSE 240

Query: 241 SEVRQLTAKELLVVRLEKQYPDDVGIIAAFFLNHVKLNPGEALYLGANEPHAYISGECIE 300
           SEVRQLTAKELLV+RLEKQYPDDVGIIAAFFLNHVKLNPGEALYLGANEPHAYISGECIE
Sbjct: 241 SEVRQLTAKELLVLRLEKQYPDDVGIIAAFFLNHVKLNPGEALYLGANEPHAYISGECIE 300

Query: 301 CMATSDNVIRAGLTPKFRDVHTLCAMFTYKQGFPEILQGVHVNQYITKYVPPFDEFEVDR 360
           CMATSDNV+RAGLTPKFRDVHTLCAMFTYKQGFPEILQGVHVNQYITKYVPPFDEFEVDR
Sbjct: 301 CMATSDNVVRAGLTPKFRDVHTLCAMFTYKQGFPEILQGVHVNQYITKYVPPFDEFEVDR 360

Query: 361 CVLPKGASVSFPASQGPSIFLVAGGEGRMLPQESLRSDVISEGDVLFVPAKTGINITSNV 420
           CVLPKGASVSFPASQGPSIFLVAGGEGRMLPQESLRSDVISEGDVLFVPAKTGINITSNV
Sbjct: 361 CVLPKGASVSFPASQGPSIFLVAGGEGRMLPQESLRSDVISEGDVLFVPAKTGINITSNV 420

Query: 421 ELLIFRAGEN 431
           ELLIFRAG N
Sbjct: 421 ELLIFRAGVN 429

BLAST of CmoCh01G004000 vs. NCBI nr
Match: XP_023523214.1 (mannose-6-phosphate isomerase 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 819.3 bits (2115), Expect = 2.7e-233
Identity = 415/430 (96.51%), Postives = 421/430 (97.91%), Query Frame = 0

Query: 1   MESDPVLTQKQSNVRRLKCSVQNYDWGIRGHDSLVARLFALNSGSSVASHKPYAEFWMGT 60
           MESDPVLTQKQSNVRRLKCSVQNYDWGIRGHDSLVARLFALNSGSSVASHKPYAEFWMGT
Sbjct: 1   MESDPVLTQKQSNVRRLKCSVQNYDWGIRGHDSLVARLFALNSGSSVASHKPYAEFWMGT 60

Query: 61  HDSGPSFVIAAAADNGGAIDSDSISLKSWVLENPNVLGDKVVEKWGSDIPFLFKVLSVAK 120
           HDSGPSF+I AAADNGGAIDS S SLKSWV ENPNVLGDKVVEKWGSDIPFLFKVLSVAK
Sbjct: 61  HDSGPSFLI-AAADNGGAIDSVSTSLKSWVSENPNVLGDKVVEKWGSDIPFLFKVLSVAK 120

Query: 121 PLSIQAHPDKELARLLHKVEPKVYRDANHKPEMALAITEFEALCGFISVEELKDVLRSVP 180
           PLSIQAHPDKELA+LLHKVEPKVYRD NHKPEMALAITEFEALCGFIS EELKDVLRSVP
Sbjct: 121 PLSIQAHPDKELAKLLHKVEPKVYRDPNHKPEMALAITEFEALCGFISGEELKDVLRSVP 180

Query: 181 EILVMIRDADRNRVLLLDDPANKEADLKAALRLAFTQLMSASKILISRAISDLKRRLHSE 240
           EILVMIRDADRNRVLLLDD A+KE DLKAALRLAFTQLMSASKILISRAISDLKRRLHSE
Sbjct: 181 EILVMIRDADRNRVLLLDDHASKEEDLKAALRLAFTQLMSASKILISRAISDLKRRLHSE 240

Query: 241 SEVRQLTAKELLVVRLEKQYPDDVGIIAAFFLNHVKLNPGEALYLGANEPHAYISGECIE 300
           SEVRQLTAKELLV+RLEKQYPDDVGII+AFFLNHVKLNPGEALYLGANEPHAYISGECIE
Sbjct: 241 SEVRQLTAKELLVLRLEKQYPDDVGIISAFFLNHVKLNPGEALYLGANEPHAYISGECIE 300

Query: 301 CMATSDNVIRAGLTPKFRDVHTLCAMFTYKQGFPEILQGVHVNQYITKYVPPFDEFEVDR 360
           CMATSDNV+RAGLTPKFRDVHTLCAMFTYKQGFPEILQGVHVNQYITKYVPPFDEFEVDR
Sbjct: 301 CMATSDNVVRAGLTPKFRDVHTLCAMFTYKQGFPEILQGVHVNQYITKYVPPFDEFEVDR 360

Query: 361 CVLPKGASVSFPASQGPSIFLVAGGEGRMLPQESLRSDVISEGDVLFVPAKTGINITSNV 420
           CVLPKGASVSFPASQGPSIFLVAGGEGRMLPQESLRSDVISEGDVLFVPAKTGINITSNV
Sbjct: 361 CVLPKGASVSFPASQGPSIFLVAGGEGRMLPQESLRSDVISEGDVLFVPAKTGINITSNV 420

Query: 421 ELLIFRAGEN 431
           ELLIFRAG N
Sbjct: 421 ELLIFRAGVN 429

BLAST of CmoCh01G004000 vs. NCBI nr
Match: XP_022997667.1 (mannose-6-phosphate isomerase 1-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 816.2 bits (2107), Expect = 2.3e-232
Identity = 411/430 (95.58%), Postives = 420/430 (97.67%), Query Frame = 0

Query: 1   MESDPVLTQKQSNVRRLKCSVQNYDWGIRGHDSLVARLFALNSGSSVASHKPYAEFWMGT 60
           MESDPVLT KQSNVRRLKCSVQNYDWGIRGHDSLVARLFALNSGSSVASHKPYAEFWMGT
Sbjct: 1   MESDPVLTPKQSNVRRLKCSVQNYDWGIRGHDSLVARLFALNSGSSVASHKPYAEFWMGT 60

Query: 61  HDSGPSFVIAAAADNGGAIDSDSISLKSWVLENPNVLGDKVVEKWGSDIPFLFKVLSVAK 120
           HDSGPSFVI AAADNGGAIDSDS SLKSWV ENPNVLGDKV EKWGSDIPFLFKVLSVAK
Sbjct: 61  HDSGPSFVI-AAADNGGAIDSDSTSLKSWVSENPNVLGDKVAEKWGSDIPFLFKVLSVAK 120

Query: 121 PLSIQAHPDKELARLLHKVEPKVYRDANHKPEMALAITEFEALCGFISVEELKDVLRSVP 180
           PLSIQAHPDKELA+LLHKVEPKVYRDANHKPEMALAITEFEALCGFISVEELKDVLRSVP
Sbjct: 121 PLSIQAHPDKELAKLLHKVEPKVYRDANHKPEMALAITEFEALCGFISVEELKDVLRSVP 180

Query: 181 EILVMIRDADRNRVLLLDDPANKEADLKAALRLAFTQLMSASKILISRAISDLKRRLHSE 240
           EIL+MIRDADRNR+LLLDD ANKE DLKAALRLAFTQLMSASKILISRAISDLKRRLHSE
Sbjct: 181 EILLMIRDADRNRLLLLDDHANKEEDLKAALRLAFTQLMSASKILISRAISDLKRRLHSE 240

Query: 241 SEVRQLTAKELLVVRLEKQYPDDVGIIAAFFLNHVKLNPGEALYLGANEPHAYISGECIE 300
           SEVRQLTAKELLV+RLEKQYPDDVGIIAAFFLNHVKLNPGEALYLGANEPHAYISGECIE
Sbjct: 241 SEVRQLTAKELLVLRLEKQYPDDVGIIAAFFLNHVKLNPGEALYLGANEPHAYISGECIE 300

Query: 301 CMATSDNVIRAGLTPKFRDVHTLCAMFTYKQGFPEILQGVHVNQYITKYVPPFDEFEVDR 360
           CMATSDNV+RAGLTPKFRDVHTLCA+FTYKQGFPEILQGVHVNQYITKYVPPF+EFEVDR
Sbjct: 301 CMATSDNVVRAGLTPKFRDVHTLCAIFTYKQGFPEILQGVHVNQYITKYVPPFEEFEVDR 360

Query: 361 CVLPKGASVSFPASQGPSIFLVAGGEGRMLPQESLRSDVISEGDVLFVPAKTGINITSNV 420
           CVLPKGASV FP SQGPSIFLVAGGEGRMLPQ+SLR+DVISEGDVLFVPAKTGINITSNV
Sbjct: 361 CVLPKGASVLFPVSQGPSIFLVAGGEGRMLPQDSLRNDVISEGDVLFVPAKTGINITSNV 420

Query: 421 ELLIFRAGEN 431
           ELLIFRAG N
Sbjct: 421 ELLIFRAGVN 429

BLAST of CmoCh01G004000 vs. NCBI nr
Match: KAG7036726.1 (Mannose-6-phosphate isomerase 1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 798.1 bits (2060), Expect = 6.4e-227
Identity = 403/412 (97.82%), Postives = 407/412 (98.79%), Query Frame = 0

Query: 1   MESDPVLTQKQSNVRRLKCSVQNYDWGIRGHDSLVARLFALNSGSSVASHKPYAEFWMGT 60
           MESDPVLTQKQSNVRRLKCSVQNYDWGIRGHDSLVARLFALNSGSSVASHKPYAEFWMGT
Sbjct: 1   MESDPVLTQKQSNVRRLKCSVQNYDWGIRGHDSLVARLFALNSGSSVASHKPYAEFWMGT 60

Query: 61  HDSGPSFVIAAAADNGGAIDSDSISLKSWVLENPNVLGDKVVEKWGSDIPFLFKVLSVAK 120
           HDSGPSFVI AAADNGGAIDSDSISLKSWVLENPNVLGDKVVEKWGSDIPFLFKVLSVAK
Sbjct: 61  HDSGPSFVI-AAADNGGAIDSDSISLKSWVLENPNVLGDKVVEKWGSDIPFLFKVLSVAK 120

Query: 121 PLSIQAHPDKELARLLHKVEPKVYRDANHKPEMALAITEFEALCGFISVEELKDVLRSVP 180
           PLSIQAHPDKELA+LLHKVEPKVYRDANHKPEMALAITEFEALCGFISVEEL DVLRSVP
Sbjct: 121 PLSIQAHPDKELAKLLHKVEPKVYRDANHKPEMALAITEFEALCGFISVEELMDVLRSVP 180

Query: 181 EILVMIRDADRNRVLLLDDPANKEADLKAALRLAFTQLMSASKILISRAISDLKRRLHSE 240
           EILVMIRDADRNRVLLLDD A KE DLKAALRLAFTQLMSASKILISRAISDLKRRLHSE
Sbjct: 181 EILVMIRDADRNRVLLLDDHATKEEDLKAALRLAFTQLMSASKILISRAISDLKRRLHSE 240

Query: 241 SEVRQLTAKELLVVRLEKQYPDDVGIIAAFFLNHVKLNPGEALYLGANEPHAYISGECIE 300
           SEVRQLTAKELLV+RLEKQYPDDVGIIAAFFLNHVKLNPGEALYLGANEPHAYISGECIE
Sbjct: 241 SEVRQLTAKELLVLRLEKQYPDDVGIIAAFFLNHVKLNPGEALYLGANEPHAYISGECIE 300

Query: 301 CMATSDNVIRAGLTPKFRDVHTLCAMFTYKQGFPEILQGVHVNQYITKYVPPFDEFEVDR 360
           CMATSDNV+RAGLTPKFRDVHTLCAMFTYKQGFPEILQGVHVNQYITKYVPPFDEFEVDR
Sbjct: 301 CMATSDNVVRAGLTPKFRDVHTLCAMFTYKQGFPEILQGVHVNQYITKYVPPFDEFEVDR 360

Query: 361 CVLPKGASVSFPASQGPSIFLVAGGEGRMLPQESLRSDVISEGDVLFVPAKT 413
           CVLPKGASVSFPASQGPSIFLVAGGEGRMLPQESLRSDVISEGDVLFVPAK+
Sbjct: 361 CVLPKGASVSFPASQGPSIFLVAGGEGRMLPQESLRSDVISEGDVLFVPAKS 411

BLAST of CmoCh01G004000 vs. TAIR 10
Match: AT3G02570.1 (Mannose-6-phosphate isomerase, type I )

HSP 1 Score: 495.4 bits (1274), Expect = 8.2e-140
Identity = 242/417 (58.03%), Postives = 315/417 (75.54%), Query Frame = 0

Query: 14  VRRLKCSVQNYDWGIRGHDSLVARLFALNSGSSVASHKPYAEFWMGTHDSGPSFVIAAAA 73
           +RRL+CSV++YDWG  G DSLV R++A NS   +   +PYAE WMGTH+SGPS++  A  
Sbjct: 20  LRRLRCSVKDYDWGKIGSDSLVYRVYAANSDYEIDPTRPYAELWMGTHESGPSYLEDADG 79

Query: 74  DNGGAIDSDSISLKSWVLENPNVLGDKVVEKWGSDIPFLFKVLSVAKPLSIQAHPDKELA 133
            NG       ++L+SW+ ENP  LG++V+EKWG D+PFLFKVLSVA+PLSIQAHPDK+LA
Sbjct: 80  SNG-------VTLRSWITENPKSLGNRVLEKWGCDLPFLFKVLSVARPLSIQAHPDKKLA 139

Query: 134 RLLHKVEPKVYRDANHKPEMALAITEFEALCGFISVEELKDVLRSVPEILVMIRDADRNR 193
           + +HK  P +Y+D NHKPEMALA T+FEALCGFI ++ELK V+R++PEI  ++   + N+
Sbjct: 140 KKMHKAHPNLYKDDNHKPEMALAYTQFEALCGFIPLQELKSVIRAIPEIEELVGSEEANQ 199

Query: 194 VLLLDDPANKEADLKAALRLAFTQLMSASKILISRAISDLKRRLHSESEVRQLTAKELLV 253
           V  + +  + E  +K+ +R  FT LMSA      + +S LKRRLH ES+ RQLT KE LV
Sbjct: 200 VFCITE--HDEEKVKSVVRTIFTLLMSADADTTKKIVSKLKRRLHMESQERQLTDKERLV 259

Query: 254 VRLEKQYPDDVGIIAAFFLNHVKLNPGEALYLGANEPHAYISGECIECMATSDNVIRAGL 313
           ++LEKQYP+D+G+I+AFF N+VKLNPGEALYLGANEPHAY+ GEC+E MATSDNV+RAGL
Sbjct: 260 LKLEKQYPNDIGVISAFFFNYVKLNPGEALYLGANEPHAYLFGECLEVMATSDNVVRAGL 319

Query: 314 TPKFRDVHTLCAMFTYKQGFPEILQGVHVNQYITKYVPPFDEFEVDRCVLPKGASVSFPA 373
           T K  D+ TLC+M +YK G+PEIL+G  +  YIT+Y+PPF+EFEVD C LP GAS  FP+
Sbjct: 320 TSKPLDIQTLCSMLSYKLGYPEILKGTRIRPYITRYLPPFEEFEVDLCDLPSGASTVFPS 379

Query: 374 SQGPSIFLVAGGEGRMLPQESLRSDVISEGDVLFVPAKTGINITSNVELLIFRAGEN 431
             GPS+ LV  GEGRM  + S  +D IS GDVLFVPA T I++ S+ +L ++RAG N
Sbjct: 380 VPGPSLLLVLQGEGRMSTEAS--ADGISMGDVLFVPADTEIHLRSSSDLKLYRAGIN 425

BLAST of CmoCh01G004000 vs. TAIR 10
Match: AT1G67070.1 (Mannose-6-phosphate isomerase, type I )

HSP 1 Score: 471.1 bits (1211), Expect = 1.7e-132
Identity = 233/424 (54.95%), Postives = 313/424 (73.82%), Query Frame = 0

Query: 14  VRRLKCSVQNYDWGIRGHDSLVARLFALNSGSSVASHKPYAEFWMGTHDSGPSFVIAAAA 73
           ++RL+C V+NY+WG  G +SLVARL   N+G  V S  PYAEFWMGTH+SGPS V     
Sbjct: 22  IQRLRCFVKNYEWGKLGPESLVARLQEANTGQRVDSEIPYAEFWMGTHESGPSHV--EFG 81

Query: 74  DNGGAIDSDSISLKSWVLENPNVLGDKVVEKWGSDIPFLFKVLSVAKPLSIQAHPDKELA 133
              G  D   ++LKSWVL+NPN+LG KVV+KWG D+PFLFKVLSV K LSIQAHP+K LA
Sbjct: 82  SGHGVSDKCMVTLKSWVLDNPNLLGSKVVDKWGCDLPFLFKVLSVTKALSIQAHPNKALA 141

Query: 134 RLLHKVEPKVYRDANHKPEMALAITEFEALCGFISVEELKDVLRSVPEILVMIRDADRNR 193
             LH+ +P +YRD NHKPE+ALA+T F+ALCGF++++ELK+V+ +VPEI  ++     ++
Sbjct: 142 EKLHREDPLLYRDNNHKPEIALAVTPFQALCGFVTLKELKEVITNVPEITELVGSKAADQ 201

Query: 194 VLLLDDPANKEADLKAALRLAFTQLMSASKILISRAISDLKRRLHSESEVRQLTAKELLV 253
           +  + +  +++  +K+ +RL FTQLMSAS     + +S +K RL  E++ R+L+ KE LV
Sbjct: 202 IFNVHE-HDEDERIKSVVRLIFTQLMSASNNETKQVVSRMKNRLLLETKHRELSEKEKLV 261

Query: 254 VRLEKQYPDDVGIIAAFFLNHVKLNPGEALYLGANEPHAYISGECIECMATSDNVIRAGL 313
           + LEKQY  D+G+I+AFF N+VKLNPGEALYL ANEPHAYISG+C+ECMA SDNV+RAGL
Sbjct: 262 LELEKQYTGDIGVISAFFFNYVKLNPGEALYLDANEPHAYISGDCVECMAASDNVVRAGL 321

Query: 314 TPKFRDVHTLCAMFTYKQGFPEILQGVHVNQYITKYVPPFDEFEVDRCVLPKGASVSFPA 373
           TPK RDV TLC+M TYK G+PEIL+G  +  Y+T+Y+PPFDEFEVD C LP+G S  FPA
Sbjct: 322 TPKHRDVQTLCSMLTYKLGYPEILKGFPLTPYVTRYLPPFDEFEVDHCDLPRGKSTVFPA 381

Query: 374 SQGPSIFLVAGGEGRMLPQESLRSDVISEGDVLFVPAKTGINITSNVELL-IFRAGENEL 433
             GPS++LV  G+G++    S    +++ GDVLFVPA   I++T   +++ ++RAG +  
Sbjct: 382 VPGPSVYLVIEGKGQLRTGSS--KVLVNRGDVLFVPADIEIHVTGESDVMKLYRAGVSSR 440

Query: 434 HPQT 437
             QT
Sbjct: 442 FFQT 440

BLAST of CmoCh01G004000 vs. TAIR 10
Match: AT1G73830.1 (BR enhanced expression 3 )

HSP 1 Score: 126.7 bits (317), Expect = 7.7e-29
Identity = 87/211 (41.23%), Postives = 119/211 (56.40%), Query Frame = 0

Query: 448 MIEFRQDLHHFKSSKASEERGL-NMEVINQFPN--MTESMLEDFNETDFSEENFLGYHFQ 507
           + E    LHHF++        L ++   NQFP+    +S+  +F++  F   N       
Sbjct: 20  LAELSNTLHHFQTFPPPFSSSLDSLFFHNQFPDHFPGKSLENNFHQGIFFPSNIQNNEES 79

Query: 508 LPENTTRSSISDFPRVALFKCQNEFPVITRSSPLVDSSQETMNRKAKQVLNLGGRKRRKC 567
             +  T+   S    V+    +N     T S+    S+Q ++N       N   R +R  
Sbjct: 80  SSQFDTKKRKSLMEAVS--TSENSVSDQTLST---SSAQVSINGNISTKNNSSRRGKRSK 139

Query: 568 EKEQEKPAEVVHIRAKRGEATHSHSLAERVRREKINNKLKCLQNLVPGCHKTMGMTMVLD 627
            +E+EK  EVVH+RA+RG+AT SHS+AERVRR KIN +LKCLQ++VPGC+KTMGM  +LD
Sbjct: 140 NREEEKEREVVHVRARRGQATDSHSIAERVRRGKINERLKCLQDIVPGCYKTMGMATMLD 199

Query: 628 ETITYVHSLQNQVEFLSMELATACSTLGINS 656
           E I YV SLQNQVEFLSM+L  A S    NS
Sbjct: 200 EIINYVQSLQNQVEFLSMKLTAASSYYDFNS 225

BLAST of CmoCh01G004000 vs. TAIR 10
Match: AT1G73830.2 (BR enhanced expression 3 )

HSP 1 Score: 126.3 bits (316), Expect = 1.0e-28
Identity = 89/229 (38.86%), Postives = 123/229 (53.71%), Query Frame = 0

Query: 448 MIEFRQDLHHFKSSKASEERGL-NMEVINQFPN--MTESMLEDFNETDFSEENFLGYHFQ 507
           + E    LHHF++        L ++   NQFP+    +S+  +F++  F   N       
Sbjct: 20  LAELSNTLHHFQTFPPPFSSSLDSLFFHNQFPDHFPGKSLENNFHQGIFFPSNIQNNEES 79

Query: 508 LPENTTRSSISDFPRVALFKCQNEFPVITRSSPLVDSSQETMNRKAKQVLNLGGRKRRKC 567
             +  T+   S    V+    +N     T S+    S+Q ++N       N   R +R  
Sbjct: 80  SSQFDTKKRKSLMEAVS--TSENSVSDQTLST---SSAQVSINGNISTKNNSSRRGKRSK 139

Query: 568 EKEQEKPAEVVHIRAKRGEATHSHSLAERVRREKINNKLKCLQNLVPGCHKTMGMTMVLD 627
            +E+EK  EVVH+RA+RG+AT SHS+AERVRR KIN +LKCLQ++VPGC+KTMGM  +LD
Sbjct: 140 NREEEKEREVVHVRARRGQATDSHSIAERVRRGKINERLKCLQDIVPGCYKTMGMATMLD 199

Query: 628 ETITYVHSLQNQVEFLSMELATACSTLGINSGMGATRKASTETIRGLAM 674
           E I YV SLQNQVEFLSM+L  A S    NS   A      +    + M
Sbjct: 200 EIINYVQSLQNQVEFLSMKLTAASSYYDFNSETDAVESMQAKAREAVEM 243

BLAST of CmoCh01G004000 vs. TAIR 10
Match: AT1G25330.1 (basic helix-loop-helix (bHLH) DNA-binding superfamily protein )

HSP 1 Score: 123.2 bits (308), Expect = 8.5e-28
Identity = 65/107 (60.75%), Postives = 82/107 (76.64%), Query Frame = 0

Query: 557 GGRKRRKCEKEQE--------KPAEVVHIRAKRGEATHSHSLAERVRREKINNKLKCLQN 616
           G ++RRK  +E+E        KP +VVH+RAKRG+AT SHSLAERVRREKIN +LKCLQ+
Sbjct: 76  GSKRRRKRSEEEEAMNGDETQKPKDVVHVRAKRGQATDSHSLAERVRREKINERLKCLQD 135

Query: 617 LVPGCHKTMGMTMVLDETITYVHSLQNQVEFLSMELATACSTLGINS 656
           LVPGC+K MGM ++LD  I YV SLQNQ+EFLSM+L+ A +   +NS
Sbjct: 136 LVPGCYKAMGMAVMLDVIIDYVRSLQNQIEFLSMKLSAASACYDLNS 182

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9M8841.2e-13858.03Mannose-6-phosphate isomerase 1 OS=Arabidopsis thaliana OX=3702 GN=PMI1 PE=1 SV=... [more]
Q9FZH52.3e-13154.95Mannose-6-phosphate isomerase 2 OS=Arabidopsis thaliana OX=3702 GN=PMI2 PE=1 SV=... [more]
Q8HXX29.0e-7539.48Mannose-6-phosphate isomerase OS=Macaca fascicularis OX=9541 GN=MPI PE=2 SV=3[more]
Q54PA03.8e-7340.24Probable mannose-6-phosphate isomerase OS=Dictyostelium discoideum OX=44689 GN=m... [more]
P349496.5e-7338.95Mannose-6-phosphate isomerase OS=Homo sapiens OX=9606 GN=MPI PE=1 SV=2[more]
Match NameE-valueIdentityDescription
A0A6J1G9H76.8e-24399.77Mannose-6-phosphate isomerase OS=Cucurbita moschata OX=3662 GN=LOC111452034 PE=3... [more]
A0A6J1K5R11.1e-23295.58Mannose-6-phosphate isomerase OS=Cucurbita maxima OX=3661 GN=LOC111492565 PE=3 S... [more]
A0A6J1KEL23.9e-22295.38Mannose-6-phosphate isomerase OS=Cucurbita maxima OX=3661 GN=LOC111492565 PE=3 S... [more]
A0A6J1CQ991.3e-19683.02Mannose-6-phosphate isomerase OS=Momordica charantia OX=3673 GN=LOC111013172 PE=... [more]
A0A6J1J7Z83.7e-19682.33Mannose-6-phosphate isomerase OS=Cucurbita maxima OX=3661 GN=LOC111482189 PE=3 S... [more]
Match NameE-valueIdentityDescription
XP_022948320.11.4e-24299.77mannose-6-phosphate isomerase 1-like [Cucurbita moschata][more]
KAG6607030.13.0e-23797.91Mannose-6-phosphate isomerase 1, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_023523214.12.7e-23396.51mannose-6-phosphate isomerase 1-like [Cucurbita pepo subsp. pepo][more]
XP_022997667.12.3e-23295.58mannose-6-phosphate isomerase 1-like isoform X1 [Cucurbita maxima][more]
KAG7036726.16.4e-22797.82Mannose-6-phosphate isomerase 1, partial [Cucurbita argyrosperma subsp. argyrosp... [more]
Match NameE-valueIdentityDescription
AT3G02570.18.2e-14058.03Mannose-6-phosphate isomerase, type I [more]
AT1G67070.11.7e-13254.95Mannose-6-phosphate isomerase, type I [more]
AT1G73830.17.7e-2941.23BR enhanced expression 3 [more]
AT1G73830.21.0e-2838.86BR enhanced expression 3 [more]
AT1G25330.18.5e-2860.75basic helix-loop-helix (bHLH) DNA-binding superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR016305Mannose-6-phosphate isomerasePRINTSPR00714MAN6PISMRASEcoord: 109..130
score: 73.64
coord: 256..271
score: 40.62
coord: 272..291
score: 60.0
coord: 291..310
score: 69.5
coord: 144..167
score: 64.17
coord: 310..329
score: 58.0
coord: 17..35
score: 47.37
IPR016305Mannose-6-phosphate isomerasePANTHERPTHR10309MANNOSE-6-PHOSPHATE ISOMERASEcoord: 8..431
IPR011598Myc-type, basic helix-loop-helix (bHLH) domainSMARTSM00353finuluscoord: 589..639
e-value: 5.7E-8
score: 42.5
IPR011598Myc-type, basic helix-loop-helix (bHLH) domainPFAMPF00010HLHcoord: 587..634
e-value: 3.0E-6
score: 27.1
IPR011598Myc-type, basic helix-loop-helix (bHLH) domainPROSITEPS50888BHLHcoord: 583..633
score: 14.138298
IPR014710RmlC-like jelly roll foldGENE3D2.60.120.10Jelly Rollscoord: 18..311
e-value: 3.0E-132
score: 443.8
IPR014710RmlC-like jelly roll foldGENE3D2.60.120.10Jelly Rollscoord: 16..427
e-value: 3.0E-132
score: 443.8
NoneNo IPR availableGENE3D1.10.441.10Phosphomannose Isomerase, domain 2coord: 168..335
e-value: 3.0E-132
score: 443.8
NoneNo IPR availablePIRSRPIRSR001480-1PIRSR001480-1coord: 22..427
e-value: 4.8E-120
score: 399.3
NoneNo IPR availablePIRSRPIRSR001480-2PIRSR001480-2coord: 22..427
e-value: 4.8E-120
score: 399.3
NoneNo IPR availablePANTHERPTHR10309:SF10MANNOSE-6-PHOSPHATE ISOMERASEcoord: 8..431
NoneNo IPR availableCDDcd07011cupin_PMI_type_I_Ncoord: 17..329
e-value: 1.89811E-119
score: 356.474
IPR001250Mannose-6-phosphate isomerase, type ITIGRFAMTIGR00218TIGR00218coord: 12..424
e-value: 3.8E-76
score: 254.5
IPR001250Mannose-6-phosphate isomerase, type IPFAMPF01238PMI_typeIcoord: 16..389
e-value: 5.4E-103
score: 345.1
IPR036638Helix-loop-helix DNA-binding domain superfamilyGENE3D4.10.280.10coord: 579..642
e-value: 1.3E-13
score: 52.4
IPR036638Helix-loop-helix DNA-binding domain superfamilySUPERFAMILY47459HLH, helix-loop-helix DNA-binding domaincoord: 581..645
IPR018050Phosphomannose isomerase, type I, conserved sitePROSITEPS00965PMI_I_1coord: 144..152
IPR018050Phosphomannose isomerase, type I, conserved sitePROSITEPS00966PMI_I_2coord: 291..316
IPR011051RmlC-like cupin domain superfamilySUPERFAMILY51182RmlC-like cupinscoord: 14..419

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh01G004000.1CmoCh01G004000.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005975 carbohydrate metabolic process
biological_process GO:0000032 cell wall mannoprotein biosynthetic process
biological_process GO:0009298 GDP-mannose biosynthetic process
biological_process GO:0006486 protein glycosylation
cellular_component GO:0005829 cytosol
molecular_function GO:0004476 mannose-6-phosphate isomerase activity
molecular_function GO:0046983 protein dimerization activity
molecular_function GO:0008270 zinc ion binding