CmoCh01G003640 (gene) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh01G003640
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
DescriptionRING-type E3 ubiquitin transferase
LocationCmo_Chr01: 1769131 .. 1771542 (+)
RNA-Seq ExpressionCmoCh01G003640
SyntenyCmoCh01G003640
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AAAACTTTTTTTTTTTTTCACGACGGAAAGACTTAGATGATCTGCGAAGTCTTCGTCCTCATGGTCTGACGAAGAGCAGAGACTAACTTGAAGAAGAACAAATGGCGGATTCTCGATCGTCGGACGGCGAAAAGGAGAGGCGGCGTTCTGTCAACTGTAGCAGAGGGAAGAACGAGGCGATGAAAGTCATATTCACTGATCCTCAAATTCTCGATTGCTATATTTGTTGCGAGCCCTTGTCCGTTCCTGTTTACCAGGTCCGATTAAGCTTTTTGATCGCGTGTATTTTGCTTGCAATTATCGTTATTATTTATCTGTTTGTCTCTGATGCTTTGTTCTTCTCGTCCTTTTTTTTTTTTTTTTTTTGTTGCAATTTTGACTACTGTAGTTCACTTAGTGTTTTTGCTGGAATTGACGATTTTGGAATCCTCCTTTCTATGAGGTTTTGATTTTTTTTTGTGTAAATCCACAGAAGTTATGGATTATGATAACAATTGAAGAAATATAGCGAAGTTTTAAATGGTTATTCATTGCGAACCTATCCAATGGTACGATGTTGTCTACTTTAAACATAAGCTCTCGTACTTTGTTTTTGGTTTTCCAAAAGGCCTCGTACCAACAGAAATAGTGTTACTTACCTGTAAAATCATGATTTTCCTCTTAATTAGCTAATCTAGACTACTCCTTTCAATAATCCTCGACAATCCTCCCATCGAACAAAGGACACCATAGAGTCTCCTCTGAGGCCTATAGAGCCATCGAACAACCTCTCTTTAATTGAGGCTGGACTCCTTTCTTTGGAGCCTCTAAACAAAATACACCTTTTGTTCGACACTTGAGTCACTTTTGACTACACTTTCGAAACTCACTTCTTTGTTCGACACTTGAGGATTCTATTGACATAGCTAAGTTAAGGGCATAACTCTTATATAATGTTAGGAATCACGAACCTCCACAATGATATGATTTTGTCCACTCTGAGCATAAGCTCTCTTGACTTTGCTTTTGGTTTCTCCAAAAAGCCTCGTGGAAAATCATGAAAATTTACTTATAACCTCATGATCTTACTTTTAATTAGCATAACGTGAGACTTTCAATCATCTTGAAAACAGTTATCAAAACATGTTTTGATTCAAATAATCTTTGAACATGGAGATTATTCTGGATATTACGGGGACAGAGTTGTTAAATGGATGGGATTGAAATGAAATCTCGACCTGGATTGCTTATCTTGTTACATTGTCTCGAGAAAATGTCAGAATCAAGTCGTGCTTGATATTCTTTTCACCTTTTATGCCTGACTTGGGGTTCAATCGTGTGGATTGTGGGATTTTCTTCATTGTTTACAAACATGAATTTGAAAGGGATGAACCTAGAAGAAAATAAGCTGATATTGGTGTATATAGTATATCTCATTTTGTTTTCTATGAGTCTTTTATTTCATAACATATACTCTGGTGTTGAGATGTTGGTTGTTGGATGAATTAGTGTGAGAACGGGCATATTTCTTGCTCTAGCTGCTGCTCCAAAGTTCACAATAAATGCCCAAGCTGCAGCTTGGCCATCGGCTACATACGTTGCAGAGCGATCGAGAAGGTTCTTGAATCAATCAAGATACCATGCCAAAACTCAACATATGGATGCAAAATCACAATGAATTTGAACCAGATAAACGAGCACGAGACATCGTGCCCATATGAGCCCTGTTCGTGCCCTATATCTGATTGCTCTTATGTTGATTCGGCAGAGCATTTGGGGTCACACTTCAGAAAAAGGCACCCAGACTCAGCCAAAAGCTTCTTATACAATTCAAGGTTCACAATATGCTTGAACAGTGGTGATAGATATCGTATCTTTCAAGCAGAGAACGATGATGCTGTGTTTGTCCTGTCTTACTCGTTTGAAATCTTTGGCAATGCGGTTACTTTGAGCCGGATTGGATCTCCGTTGTGTGAGAAGAAGTTTTGTTATGATATCAAAGCCAAATCACAGGGGAGTGTTCTTTCTTTGCAATCAGTGGCGAATGAGATCCGACGTTGGATTGAAGATCCTCCTTTCAAGGGATCGCTTCTGATTCCAAATGAGTTCTTCGGATCTTCTTCAGCCCAGACGATCTTGGAGATTCGCATTTGGCCTTCACGCTGAAGATAAACACTGGGGATCAACACAGGTGCACATACATTTTTTTTGAAACAAAGCTGAATTATTGTTTAAACTGAGTTAATCTTTGCATCTGTGTTTTCTTTGTTTTTTTGGGATGGACTTTGGTGCATACTTTTGTGCATATATATCTATATGGGTTTGTTGAACTTTTAATTTTGTATGGAATAGAACTAAGCTTGTGAATGTTTGTGAATTAAAAAAAAAAAAAAATGTTGAGTGAGTCTATGGCCGATATCGCTCAGTTGGTAG

mRNA sequence

AAAACTTTTTTTTTTTTTCACGACGGAAAGACTTAGATGATCTGCGAAGTCTTCGTCCTCATGGTCTGACGAAGAGCAGAGACTAACTTGAAGAAGAACAAATGGCGGATTCTCGATCGTCGGACGGCGAAAAGGAGAGGCGGCGTTCTGTCAACTGTAGCAGAGGGAAGAACGAGGCGATGAAAGTCATATTCACTGATCCTCAAATTCTCGATTGCTATATTTGTTGCGAGCCCTTGTCCGTTCCTGTTTACCAGTGTGAGAACGGGCATATTTCTTGCTCTAGCTGCTGCTCCAAAGTTCACAATAAATGCCCAAGCTGCAGCTTGGCCATCGGCTACATACGTTGCAGAGCGATCGAGAAGGTTCTTGAATCAATCAAGATACCATGCCAAAACTCAACATATGGATGCAAAATCACAATGAATTTGAACCAGATAAACGAGCACGAGACATCGTGCCCATATGAGCCCTGTTCGTGCCCTATATCTGATTGCTCTTATGTTGATTCGGCAGAGCATTTGGGGTCACACTTCAGAAAAAGGCACCCAGACTCAGCCAAAAGCTTCTTATACAATTCAAGGTTCACAATATGCTTGAACAGTGGTGATAGATATCGTATCTTTCAAGCAGAGAACGATGATGCTGTGTTTGTCCTGTCTTACTCGTTTGAAATCTTTGGCAATGCGGTTACTTTGAGCCGGATTGGATCTCCGTTGTGTGAGAAGAAGTTTTGTTATGATATCAAAGCCAAATCACAGGGGAGTGTTCTTTCTTTGCAATCAGTGGCGAATGAGATCCGACGTTGGATTGAAGATCCTCCTTTCAAGGGATCGCTTCTGATTCCAAATGAGTTCTTCGGATCTTCTTCAGCCCAGACGATCTTGGAGATTCGCATTTGGCCTTCACGCTGAAGATAAACACTGGGGATCAACACAGGTGCACATACATTTTTTTTGAAACAAAGCTGAATTATTGTTTAAACTGAGTTAATCTTTGCATCTGTGTTTTCTTTGTTTTTTTGGGATGGACTTTGGTGCATACTTTTGTGCATATATATCTATATGGGTTTGTTGAACTTTTAATTTTGTATGGAATAGAACTAAGCTTGTGAATGTTTGTGAATTAAAAAAAAAAAAAAATGTTGAGTGAGTCTATGGCCGATATCGCTCAGTTGGTAG

Coding sequence (CDS)

ATGGCGGATTCTCGATCGTCGGACGGCGAAAAGGAGAGGCGGCGTTCTGTCAACTGTAGCAGAGGGAAGAACGAGGCGATGAAAGTCATATTCACTGATCCTCAAATTCTCGATTGCTATATTTGTTGCGAGCCCTTGTCCGTTCCTGTTTACCAGTGTGAGAACGGGCATATTTCTTGCTCTAGCTGCTGCTCCAAAGTTCACAATAAATGCCCAAGCTGCAGCTTGGCCATCGGCTACATACGTTGCAGAGCGATCGAGAAGGTTCTTGAATCAATCAAGATACCATGCCAAAACTCAACATATGGATGCAAAATCACAATGAATTTGAACCAGATAAACGAGCACGAGACATCGTGCCCATATGAGCCCTGTTCGTGCCCTATATCTGATTGCTCTTATGTTGATTCGGCAGAGCATTTGGGGTCACACTTCAGAAAAAGGCACCCAGACTCAGCCAAAAGCTTCTTATACAATTCAAGGTTCACAATATGCTTGAACAGTGGTGATAGATATCGTATCTTTCAAGCAGAGAACGATGATGCTGTGTTTGTCCTGTCTTACTCGTTTGAAATCTTTGGCAATGCGGTTACTTTGAGCCGGATTGGATCTCCGTTGTGTGAGAAGAAGTTTTGTTATGATATCAAAGCCAAATCACAGGGGAGTGTTCTTTCTTTGCAATCAGTGGCGAATGAGATCCGACGTTGGATTGAAGATCCTCCTTTCAAGGGATCGCTTCTGATTCCAAATGAGTTCTTCGGATCTTCTTCAGCCCAGACGATCTTGGAGATTCGCATTTGGCCTTCACGCTGA

Protein sequence

MADSRSSDGEKERRRSVNCSRGKNEAMKVIFTDPQILDCYICCEPLSVPVYQCENGHISCSSCCSKVHNKCPSCSLAIGYIRCRAIEKVLESIKIPCQNSTYGCKITMNLNQINEHETSCPYEPCSCPISDCSYVDSAEHLGSHFRKRHPDSAKSFLYNSRFTICLNSGDRYRIFQAENDDAVFVLSYSFEIFGNAVTLSRIGSPLCEKKFCYDIKAKSQGSVLSLQSVANEIRRWIEDPPFKGSLLIPNEFFGSSSAQTILEIRIWPSR
Homology
BLAST of CmoCh01G003640 vs. ExPASy Swiss-Prot
Match: Q84K34 (E3 ubiquitin-protein ligase SINA-like 10 OS=Arabidopsis thaliana OX=3702 GN=At5g37930 PE=2 SV=1)

HSP 1 Score: 196.8 bits (499), Expect = 3.1e-49
Identity = 109/280 (38.93%), Postives = 152/280 (54.29%), Query Frame = 0

Query: 4   SRSSDGE---KERR-----RSVNCSRGKNEAMKVIFTDPQILDCYICCEPLSVPVYQCEN 63
           S  SD E   +ERR      S + S  K+  + V   DP +LDC ICCEPL +P++QC+N
Sbjct: 70  SDDSDREVVIEERRFGKFVNSQSSSSSKDSPLSVTLLDPDVLDCPICCEPLKIPIFQCDN 129

Query: 64  GHISCSSCCSKVHNKCPSCSLAIGYIRCRAIEKVLESIKIPCQNSTYGCKITMNLNQINE 123
           GH++C+ CC+KV N+CPSC+L IGY+RCRA+EKV+E+ ++ C N+ YGCK + +      
Sbjct: 130 GHLACTLCCTKVRNRCPSCTLPIGYVRCRAMEKVIEASRVSCLNAKYGCKESTSYGNRFS 189

Query: 124 HETSCPYEPCSCPISDCSYVDSAEHLGSHFRKRHPDSAKSFLYNSRFTICLNSGDRYRIF 183
           HE  C + PCSCPI DC Y    + L +H R  H D   SF++N+R TI L+   +  I 
Sbjct: 190 HEQVCVFTPCSCPILDCHYTGYYKDLNNHVRAEHKDDLISFVWNTRLTISLDLNKKTTIL 249

Query: 184 QAENDDAVFVLSYSFEIFGNAVTLS-------RIGSPLCE-KKFCYDIKAKSQGSVLSLQ 243
           Q END  V V+     +    V++S        +G   C   K   D   K    V ++Q
Sbjct: 250 QEENDGHVIVVQVFRALHAVYVSVSCIAPLTPGVGRLSCRLAKITVDSLLKQGFMVKNIQ 309

Query: 244 SVANEIRRWIEDPPFKGSLLIPNEFFGSSSAQTILEIRIW 268
            V NE        P  G +LIP+  F   S    L ++IW
Sbjct: 310 KVTNE-------HPEDGFMLIPSYLF---SGNDNLNLQIW 339

BLAST of CmoCh01G003640 vs. ExPASy Swiss-Prot
Match: Q9FKD7 (E3 ubiquitin-protein ligase SINA-like 7 OS=Arabidopsis thaliana OX=3702 GN=At5g37890 PE=2 SV=1)

HSP 1 Score: 154.1 bits (388), Expect = 2.3e-36
Identity = 87/269 (32.34%), Postives = 144/269 (53.53%), Query Frame = 0

Query: 4   SRSSDGEKERRRSVNCSRGKNEAMKVIFTDPQILDCYICCEPLSVPVYQCENGHISCSSC 63
           S S   +K +  S + ++   +    +  D +ILDC IC E  ++P++QC+NGH++CSSC
Sbjct: 16  SNSILSQKRQLSSSDAAKRDAKKRSTMLMDLEILDCPICYEAFTIPIFQCDNGHLACSSC 75

Query: 64  CSKVHNKCPSCSLAIGYIRCRAIEKVLESIKIPCQNSTYGCKITMNLNQINEHETSCPYE 123
           C K++NKCP+C+  +G+ RCRA+E VLESI IPC N+  GCK  ++  +   HE  C + 
Sbjct: 76  CPKLNNKCPACTSPVGHNRCRAMESVLESILIPCPNAKLGCKKNVSYGKELTHEKECMFS 135

Query: 124 PCSCPISDCSYVDSAEHLGSHFRKRHPD--SAKSFLYNSRFTICLNSGDRYRIFQAENDD 183
            C+CP  DC+Y  S + L +H+R  H +     +F+ +   ++ +N   +  I      +
Sbjct: 136 HCACPALDCNYTSSYKDLYTHYRITHMEINQINTFICDIPLSVRMNISKKILIRTEHLTN 195

Query: 184 AVFVLSYSFEIFGNAVTLSRIGSPLCE-KKFCYDIKAKSQGSVLSLQSVANEIRRWIE-- 243
            +F +    E +G  VT+S I     E  ++ Y +     G  +  QS   E++R ++  
Sbjct: 196 HLFAVQCFREPYGVYVTVSCIAPSSPELSQYSYALSYTVDGHTVIYQS--PEVKRVLKLS 255

Query: 244 -DPPFKGSLLIPNEFFGSSSAQTILEIRI 267
              P +  +LIPN          +LE+RI
Sbjct: 256 FQTPQENFMLIPNSLLRGD----VLEMRI 278

BLAST of CmoCh01G003640 vs. ExPASy Swiss-Prot
Match: Q9FM14 (E3 ubiquitin-protein ligase SINA-like 11 OS=Arabidopsis thaliana OX=3702 GN=At5g62800 PE=2 SV=2)

HSP 1 Score: 150.6 bits (379), Expect = 2.5e-35
Identity = 91/270 (33.70%), Postives = 137/270 (50.74%), Query Frame = 0

Query: 1   MADSRS-SDGEKERRRSVN---CSRGKNEAMKVIFTDPQILDCYICCEPLSVPVYQCENG 60
           M DS S    +  +R+S +     R +NE       D  +LDC +C EPL++P +QC++G
Sbjct: 1   MEDSNSHPQNQTSKRKSSHPQKKQRMENETRSAKLLDLDVLDCPVCFEPLTIPTFQCDDG 60

Query: 61  HISCSSCCSKVHNKC--PSCSLAIGYIRCRAIEKVLESIKIPCQNSTYGCKITMNLNQIN 120
           HI C+ C +KV NKC  P C L IG  RC A+E+VLES  +PCQN+ +GC  +++  +++
Sbjct: 61  HIVCNFCFAKVSNKCPGPGCDLPIGNKRCFAMERVLESAFVPCQNTEFGCTKSVSYEKVS 120

Query: 121 EHETSCPYEPCSCPISDCSYVDSAEHLGSHFRKRHPDSAKSFLYNSRFT----------I 180
            HE  C Y  CSCP  +C+Y  S   +  HF +RH       LYNS             +
Sbjct: 121 SHEKECNYSQCSCPNLECNYTGSYNIIYGHFMRRH-------LYNSTIVSSKWGYSTVDV 180

Query: 181 CLNSGDRYRIFQAENDDAVFVLSYSFEIFGNAVTLSRIGSPLCE-KKFCYDIKAKSQGSV 240
            +N  ++  +        +FV+    E  G  VT+ RI  P  E KKF Y +     G  
Sbjct: 181 LINIKEKVSVLWESRQKLLFVVQCFKERHGVYVTVRRIAPPASEFKKFSYRLSYSIDGHN 240

Query: 241 LSLQSVANEIRRWIE---DPPFKGSLLIPN 251
           ++ +S   E++R +E     P    + +PN
Sbjct: 241 VTYES--PEVKRLLEVNSQIPDDSFMFVPN 261

BLAST of CmoCh01G003640 vs. ExPASy Swiss-Prot
Match: Q9FKD9 (Putative E3 ubiquitin-protein ligase SINA-like 6 OS=Arabidopsis thaliana OX=3702 GN=At5g37870 PE=3 SV=1)

HSP 1 Score: 150.6 bits (379), Expect = 2.5e-35
Identity = 84/255 (32.94%), Postives = 136/255 (53.33%), Query Frame = 0

Query: 4   SRSSDGEKERRRSVNCSRGKNEAMK---VIFTDPQILDCYICCEPLSVPVYQCENGHISC 63
           S  +  +K    S++   G+    K    + TD  ILDC IC + L +PV+QC NGH++C
Sbjct: 7   SERNGSQKRHCSSISSDDGRKRVDKTRSAMLTDLDILDCPICYQALKIPVFQCGNGHLAC 66

Query: 64  SSCCSKVHNKCPSCSLAIGYIRCRAIEKVLESIKIPCQNSTYGCKITMNLNQINEHETSC 123
           SSCC K+ NKCP+C+L +G+IRCRA+E+VLES+ +PC+ +  GC  T+   + + HE  C
Sbjct: 67  SSCCPKLRNKCPACALPVGHIRCRAMERVLESVLVPCRYADLGCTKTIYYGRESTHEKIC 126

Query: 124 PYEPCSCPISDCSYVDSAEHLGSHFRKRHPDSAKSFLYN--SRFTICLNSGDRYRIFQAE 183
            + PCSCP+  C+Y  S + L  H+   H   + ++ +N  S     +   D+  I +  
Sbjct: 127 NFSPCSCPVQGCNYTGSYKDLYEHYDLTHSTGSTAYSFNGVSYIAAMMFISDKILIERVY 186

Query: 184 NDDAVFVLSYSFEIFGNAVTLSRIGSPLCE-KKFCYDIKAKS-QGSVLSLQS-VANEIRR 243
               +FV+    E  G  V++S I     E  +F Y +   + +G  ++ QS    ++ +
Sbjct: 187 EKKLLFVVQCFEEPCGVYVSVSCIAPSAPEVGEFSYGLLYTTWEGVTMTYQSPKVKKVLK 246

Query: 244 WIEDPPFKGSLLIPN 251
                P    +LIP+
Sbjct: 247 VSSQRPKDSFMLIPH 261

BLAST of CmoCh01G003640 vs. ExPASy Swiss-Prot
Match: Q9FKD5 (Putative E3 ubiquitin-protein ligase SINA-like 9 OS=Arabidopsis thaliana OX=3702 GN=At5g37910 PE=3 SV=1)

HSP 1 Score: 148.7 bits (374), Expect = 9.7e-35
Identity = 81/236 (34.32%), Postives = 125/236 (52.97%), Query Frame = 0

Query: 23  KNEAMKVIFTDPQILDCYICCEPLSVPVYQCENGHISCSSCCSKVHNKCPSCSLAIGYIR 82
           K +   ++  D  ILDC ICCE L+ P++QC+NGH++C SCC K+ NKCP+C+L +G+ R
Sbjct: 22  KRQRSAIVLLDLDILDCPICCEALTSPIFQCDNGHLACGSCCPKLSNKCPACTLPVGHSR 81

Query: 83  CRAIEKVLESIKIPCQNSTYGCKITMNLNQINEHETSCPYEPCSCPISDCSYVDSAEHLG 142
            RA+E VLESI IPC N  +GC  +    + + HE  C +  CSCP S C Y  S + L 
Sbjct: 82  SRAMESVLESILIPCPNVRFGCTKSFFYGKESAHEKECIFSQCSCPSSVCDYTGSYKDLY 141

Query: 143 SHFRKRHPD----SAKSFLYNSRFTICLNSGDRYRIFQAENDDAVFVLSYSFEIFGNAVT 202
           +H++  H      + K F   + FT  +   D+  I +      +  +    E  G  VT
Sbjct: 142 AHYKLTHSTNIFWNIKRFRCANFFTTSMLISDKILIKRVHEKKLLLAVQCFREPCGVYVT 201

Query: 203 LSRIGSPLCE-KKFCYDIKAKSQGSVLSLQSVANEIRRWIE---DPPFKGSLLIPN 251
           +S I     E  +F Y +     G  ++ +S   E++R  +   + P +  +LIP+
Sbjct: 202 VSFIAPSAPEVGEFSYQLSYNVDGHTVTYES--PEVKRVCKVSIETPQENFMLIPH 255

BLAST of CmoCh01G003640 vs. ExPASy TrEMBL
Match: A0A6J1GBP1 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111452508 PE=3 SV=1)

HSP 1 Score: 566.2 bits (1458), Expect = 7.2e-158
Identity = 270/270 (100.00%), Postives = 270/270 (100.00%), Query Frame = 0

Query: 1   MADSRSSDGEKERRRSVNCSRGKNEAMKVIFTDPQILDCYICCEPLSVPVYQCENGHISC 60
           MADSRSSDGEKERRRSVNCSRGKNEAMKVIFTDPQILDCYICCEPLSVPVYQCENGHISC
Sbjct: 1   MADSRSSDGEKERRRSVNCSRGKNEAMKVIFTDPQILDCYICCEPLSVPVYQCENGHISC 60

Query: 61  SSCCSKVHNKCPSCSLAIGYIRCRAIEKVLESIKIPCQNSTYGCKITMNLNQINEHETSC 120
           SSCCSKVHNKCPSCSLAIGYIRCRAIEKVLESIKIPCQNSTYGCKITMNLNQINEHETSC
Sbjct: 61  SSCCSKVHNKCPSCSLAIGYIRCRAIEKVLESIKIPCQNSTYGCKITMNLNQINEHETSC 120

Query: 121 PYEPCSCPISDCSYVDSAEHLGSHFRKRHPDSAKSFLYNSRFTICLNSGDRYRIFQAEND 180
           PYEPCSCPISDCSYVDSAEHLGSHFRKRHPDSAKSFLYNSRFTICLNSGDRYRIFQAEND
Sbjct: 121 PYEPCSCPISDCSYVDSAEHLGSHFRKRHPDSAKSFLYNSRFTICLNSGDRYRIFQAEND 180

Query: 181 DAVFVLSYSFEIFGNAVTLSRIGSPLCEKKFCYDIKAKSQGSVLSLQSVANEIRRWIEDP 240
           DAVFVLSYSFEIFGNAVTLSRIGSPLCEKKFCYDIKAKSQGSVLSLQSVANEIRRWIEDP
Sbjct: 181 DAVFVLSYSFEIFGNAVTLSRIGSPLCEKKFCYDIKAKSQGSVLSLQSVANEIRRWIEDP 240

Query: 241 PFKGSLLIPNEFFGSSSAQTILEIRIWPSR 271
           PFKGSLLIPNEFFGSSSAQTILEIRIWPSR
Sbjct: 241 PFKGSLLIPNEFFGSSSAQTILEIRIWPSR 270

BLAST of CmoCh01G003640 vs. ExPASy TrEMBL
Match: A0A6J1K8G5 (RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111492642 PE=3 SV=1)

HSP 1 Score: 550.1 bits (1416), Expect = 5.3e-153
Identity = 263/270 (97.41%), Postives = 265/270 (98.15%), Query Frame = 0

Query: 1   MADSRSSDGEKERRRSVNCSRGKNEAMKVIFTDPQILDCYICCEPLSVPVYQCENGHISC 60
           MADSRSSDGEKERRRSVNCSRGKNEAMKVIFTDPQILDCYICCEPLSVPVYQCENGHISC
Sbjct: 1   MADSRSSDGEKERRRSVNCSRGKNEAMKVIFTDPQILDCYICCEPLSVPVYQCENGHISC 60

Query: 61  SSCCSKVHNKCPSCSLAIGYIRCRAIEKVLESIKIPCQNSTYGCKITMNLNQINEHETSC 120
           SSCCSKVHNKCPSCSLAIGYIRCRAIEKVLESIKIPCQNS YGCKITMNLNQINEHETSC
Sbjct: 61  SSCCSKVHNKCPSCSLAIGYIRCRAIEKVLESIKIPCQNSKYGCKITMNLNQINEHETSC 120

Query: 121 PYEPCSCPISDCSYVDSAEHLGSHFRKRHPDSAKSFLYNSRFTICLNSGDRYRIFQAEND 180
           PYEPCSCPISDCSYVDSAEHLGSHFRKRH DSAKSFLYNSRFTICLNSGDRYRIFQAEND
Sbjct: 121 PYEPCSCPISDCSYVDSAEHLGSHFRKRHQDSAKSFLYNSRFTICLNSGDRYRIFQAEND 180

Query: 181 DAVFVLSYSFEIFGNAVTLSRIGSPLCEKKFCYDIKAKSQGSVLSLQSVANEIRRWIEDP 240
           DA+FVLSYSFEIFGNAVTLSRIGSPL EKKFCYDIKAKSQGSVLSLQSVA EI+RW EDP
Sbjct: 181 DALFVLSYSFEIFGNAVTLSRIGSPLSEKKFCYDIKAKSQGSVLSLQSVAKEIQRWTEDP 240

Query: 241 PFKGSLLIPNEFFGSSSAQTILEIRIWPSR 271
           PFKGSLLIPNEFFGSSSAQTILEIRIWPSR
Sbjct: 241 PFKGSLLIPNEFFGSSSAQTILEIRIWPSR 270

BLAST of CmoCh01G003640 vs. ExPASy TrEMBL
Match: A0A6J1KAW2 (RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111492641 PE=3 SV=1)

HSP 1 Score: 480.7 bits (1236), Expect = 4.0e-132
Identity = 230/270 (85.19%), Postives = 240/270 (88.89%), Query Frame = 0

Query: 1   MADSRSSDGEKERRRSVNCSRGKNEAMKVIFTDPQILDCYICCEPLSVPVYQCENGHISC 60
           MA SRSSDGEKERR SVN SR KNE MKVI TDPQ+LDCYICC PLSVPVYQCENGHISC
Sbjct: 1   MAGSRSSDGEKERRSSVNSSRWKNETMKVILTDPQVLDCYICCVPLSVPVYQCENGHISC 60

Query: 61  SSCCSKVHNKCPSCSLAIGYIRCRAIEKVLESIKIPCQNSTYGCKITMNLNQINEHETSC 120
           SSCCSKVHNKCPSCSL IGY+RCRAIEKVLESIK+PCQNS YGCK T NLNQINEHET C
Sbjct: 61  SSCCSKVHNKCPSCSLPIGYMRCRAIEKVLESIKMPCQNSRYGCKTTTNLNQINEHETLC 120

Query: 121 PYEPCSCPISDCSYVDSAEHLGSHFRKRHPDSAKSFLYNSRFTICLNSGDRYRIFQAEND 180
           PY+PCSCP+SDCSYVDSAEHLGSHFRKRH DSAKS  YNSRFTICLNS D+Y IFQAEND
Sbjct: 121 PYQPCSCPLSDCSYVDSAEHLGSHFRKRHQDSAKSISYNSRFTICLNSDDKYCIFQAEND 180

Query: 181 DAVFVLSYSFEIFGNAVTLSRIGSPLCEKKFCYDIKAKSQGSVLSLQSVANEIRRWIEDP 240
             +FVLSYSFE FG+AVTL RIG    EKKFCYDIKAKS G+VLSL SVA EI+RW EDP
Sbjct: 181 GVLFVLSYSFETFGDAVTLKRIGPRSSEKKFCYDIKAKSHGNVLSLLSVAKEIQRWTEDP 240

Query: 241 PFKGSLLIPNEFFGSSSAQTILEIRIWPSR 271
           P KGSLLIPNEFFGSSSAQTILEIRIWPSR
Sbjct: 241 PSKGSLLIPNEFFGSSSAQTILEIRIWPSR 270

BLAST of CmoCh01G003640 vs. ExPASy TrEMBL
Match: A0A6J1KCJ8 (RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111492641 PE=3 SV=1)

HSP 1 Score: 464.9 bits (1195), Expect = 2.3e-127
Identity = 230/300 (76.67%), Postives = 240/300 (80.00%), Query Frame = 0

Query: 1   MADSRSSDGEKERRRSVNCSRGKNEAMKVIFTDPQILDCYICCEPLSVPVYQ-------- 60
           MA SRSSDGEKERR SVN SR KNE MKVI TDPQ+LDCYICC PLSVPVYQ        
Sbjct: 1   MAGSRSSDGEKERRSSVNSSRWKNETMKVILTDPQVLDCYICCVPLSVPVYQTVDFQWKL 60

Query: 61  ----------------------CENGHISCSSCCSKVHNKCPSCSLAIGYIRCRAIEKVL 120
                                 CENGHISCSSCCSKVHNKCPSCSL IGY+RCRAIEKVL
Sbjct: 61  WIMIKIKEICRSFEQLLAKIVECENGHISCSSCCSKVHNKCPSCSLPIGYMRCRAIEKVL 120

Query: 121 ESIKIPCQNSTYGCKITMNLNQINEHETSCPYEPCSCPISDCSYVDSAEHLGSHFRKRHP 180
           ESIK+PCQNS YGCK T NLNQINEHET CPY+PCSCP+SDCSYVDSAEHLGSHFRKRH 
Sbjct: 121 ESIKMPCQNSRYGCKTTTNLNQINEHETLCPYQPCSCPLSDCSYVDSAEHLGSHFRKRHQ 180

Query: 181 DSAKSFLYNSRFTICLNSGDRYRIFQAENDDAVFVLSYSFEIFGNAVTLSRIGSPLCEKK 240
           DSAKS  YNSRFTICLNS D+Y IFQAEND  +FVLSYSFE FG+AVTL RIG    EKK
Sbjct: 181 DSAKSISYNSRFTICLNSDDKYCIFQAENDGVLFVLSYSFETFGDAVTLKRIGPRSSEKK 240

Query: 241 FCYDIKAKSQGSVLSLQSVANEIRRWIEDPPFKGSLLIPNEFFGSSSAQTILEIRIWPSR 271
           FCYDIKAKS G+VLSL SVA EI+RW EDPP KGSLLIPNEFFGSSSAQTILEIRIWPSR
Sbjct: 241 FCYDIKAKSHGNVLSLLSVAKEIQRWTEDPPSKGSLLIPNEFFGSSSAQTILEIRIWPSR 300

BLAST of CmoCh01G003640 vs. ExPASy TrEMBL
Match: A0A6J1CNI1 (RING-type E3 ubiquitin transferase OS=Momordica charantia OX=3673 GN=LOC111013226 PE=3 SV=1)

HSP 1 Score: 412.1 bits (1058), Expect = 1.7e-111
Identity = 197/269 (73.23%), Postives = 227/269 (84.39%), Query Frame = 0

Query: 1   MADSRSSDGEKERRRSVNCSRGKNEAMKVIFTDPQILDCYICCEPLSVPVYQCENGHISC 60
           MADSR+SDGE+ +R  V+  RGK EAM VIF+DPQ+LDCYICCEPLS PV+QCENGHI+C
Sbjct: 1   MADSRASDGERGKRSPVS-GRGKTEAMNVIFSDPQLLDCYICCEPLSAPVFQCENGHIAC 60

Query: 61  SSCCSKVHNKCPSCSLAIGYIRCRAIEKVLESIKIPCQNSTYGCKITMNLNQINEHETSC 120
           SSCC+KV NKCPSCSL+IGYIRCRAIEKVLESIKIPC NS YGCK+TM LN+ N+H + C
Sbjct: 61  SSCCTKVQNKCPSCSLSIGYIRCRAIEKVLESIKIPCHNSIYGCKMTMTLNERNDHGSLC 120

Query: 121 PYEPCSCPISDCSYVDSAEHLGSHFRKRHPDSAKSFLYNSRFTICLNSGDRYRIFQAEND 180
            YEPCSCP+SDC +V S+E L SHF K+H +SAKSF YNSRFT+CLNSGD YRI QAE D
Sbjct: 121 TYEPCSCPVSDCLFVGSSEKLCSHFSKKHKNSAKSFSYNSRFTLCLNSGDSYRILQAEKD 180

Query: 181 DAVFVLSYSFEIFGNAVTLSRIGSPLCEKKFCYDIKAKSQGSVLSLQSVANEIRRWIEDP 240
            A+F LS++FEIFGNAVT+SRIG    EKKFCY+IKAK+QGS+L LQS+A EI+  +E P
Sbjct: 181 GALFFLSFTFEIFGNAVTVSRIGPSSSEKKFCYEIKAKTQGSILCLQSIAKEIQGRVEVP 240

Query: 241 PFKGSLLIPNEFFGSSSAQTILEIRIWPS 270
           P   SLLIPNEFFG SSAQTILE RIWPS
Sbjct: 241 PSNRSLLIPNEFFG-SSAQTILEFRIWPS 267

BLAST of CmoCh01G003640 vs. NCBI nr
Match: XP_022949045.1 (uncharacterized protein LOC111452508 [Cucurbita moschata])

HSP 1 Score: 566.2 bits (1458), Expect = 1.5e-157
Identity = 270/270 (100.00%), Postives = 270/270 (100.00%), Query Frame = 0

Query: 1   MADSRSSDGEKERRRSVNCSRGKNEAMKVIFTDPQILDCYICCEPLSVPVYQCENGHISC 60
           MADSRSSDGEKERRRSVNCSRGKNEAMKVIFTDPQILDCYICCEPLSVPVYQCENGHISC
Sbjct: 1   MADSRSSDGEKERRRSVNCSRGKNEAMKVIFTDPQILDCYICCEPLSVPVYQCENGHISC 60

Query: 61  SSCCSKVHNKCPSCSLAIGYIRCRAIEKVLESIKIPCQNSTYGCKITMNLNQINEHETSC 120
           SSCCSKVHNKCPSCSLAIGYIRCRAIEKVLESIKIPCQNSTYGCKITMNLNQINEHETSC
Sbjct: 61  SSCCSKVHNKCPSCSLAIGYIRCRAIEKVLESIKIPCQNSTYGCKITMNLNQINEHETSC 120

Query: 121 PYEPCSCPISDCSYVDSAEHLGSHFRKRHPDSAKSFLYNSRFTICLNSGDRYRIFQAEND 180
           PYEPCSCPISDCSYVDSAEHLGSHFRKRHPDSAKSFLYNSRFTICLNSGDRYRIFQAEND
Sbjct: 121 PYEPCSCPISDCSYVDSAEHLGSHFRKRHPDSAKSFLYNSRFTICLNSGDRYRIFQAEND 180

Query: 181 DAVFVLSYSFEIFGNAVTLSRIGSPLCEKKFCYDIKAKSQGSVLSLQSVANEIRRWIEDP 240
           DAVFVLSYSFEIFGNAVTLSRIGSPLCEKKFCYDIKAKSQGSVLSLQSVANEIRRWIEDP
Sbjct: 181 DAVFVLSYSFEIFGNAVTLSRIGSPLCEKKFCYDIKAKSQGSVLSLQSVANEIRRWIEDP 240

Query: 241 PFKGSLLIPNEFFGSSSAQTILEIRIWPSR 271
           PFKGSLLIPNEFFGSSSAQTILEIRIWPSR
Sbjct: 241 PFKGSLLIPNEFFGSSSAQTILEIRIWPSR 270

BLAST of CmoCh01G003640 vs. NCBI nr
Match: KAG6606996.1 (E3 ubiquitin-protein ligase SINA-like 10, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 562.0 bits (1447), Expect = 2.8e-156
Identity = 268/270 (99.26%), Postives = 269/270 (99.63%), Query Frame = 0

Query: 1   MADSRSSDGEKERRRSVNCSRGKNEAMKVIFTDPQILDCYICCEPLSVPVYQCENGHISC 60
           MADSRSSDGEKERRRSVNCSRGKNEAMKVIFTDPQILDCYICCEPLSVPVYQCENGHISC
Sbjct: 1   MADSRSSDGEKERRRSVNCSRGKNEAMKVIFTDPQILDCYICCEPLSVPVYQCENGHISC 60

Query: 61  SSCCSKVHNKCPSCSLAIGYIRCRAIEKVLESIKIPCQNSTYGCKITMNLNQINEHETSC 120
           SSCCSKVHNKCPSCSLAIGYIRCRAIEKVLESIKIPCQNSTYGCKITMNLNQINEHETSC
Sbjct: 61  SSCCSKVHNKCPSCSLAIGYIRCRAIEKVLESIKIPCQNSTYGCKITMNLNQINEHETSC 120

Query: 121 PYEPCSCPISDCSYVDSAEHLGSHFRKRHPDSAKSFLYNSRFTICLNSGDRYRIFQAEND 180
           PYEPCSCPISDCSYVDSAEHLGSHFRKRH DSAKSFLYNSRFTICLNSGDRYRIFQAEND
Sbjct: 121 PYEPCSCPISDCSYVDSAEHLGSHFRKRHQDSAKSFLYNSRFTICLNSGDRYRIFQAEND 180

Query: 181 DAVFVLSYSFEIFGNAVTLSRIGSPLCEKKFCYDIKAKSQGSVLSLQSVANEIRRWIEDP 240
           DA+FVLSYSFEIFGNAVTLSRIGSPLCEKKFCYDIKAKSQGSVLSLQSVANEIRRWIEDP
Sbjct: 181 DALFVLSYSFEIFGNAVTLSRIGSPLCEKKFCYDIKAKSQGSVLSLQSVANEIRRWIEDP 240

Query: 241 PFKGSLLIPNEFFGSSSAQTILEIRIWPSR 271
           PFKGSLLIPNEFFGSSSAQTILEIRIWPSR
Sbjct: 241 PFKGSLLIPNEFFGSSSAQTILEIRIWPSR 270

BLAST of CmoCh01G003640 vs. NCBI nr
Match: XP_022997791.1 (E3 ubiquitin-protein ligase SINA-like 10 [Cucurbita maxima])

HSP 1 Score: 550.1 bits (1416), Expect = 1.1e-152
Identity = 263/270 (97.41%), Postives = 265/270 (98.15%), Query Frame = 0

Query: 1   MADSRSSDGEKERRRSVNCSRGKNEAMKVIFTDPQILDCYICCEPLSVPVYQCENGHISC 60
           MADSRSSDGEKERRRSVNCSRGKNEAMKVIFTDPQILDCYICCEPLSVPVYQCENGHISC
Sbjct: 1   MADSRSSDGEKERRRSVNCSRGKNEAMKVIFTDPQILDCYICCEPLSVPVYQCENGHISC 60

Query: 61  SSCCSKVHNKCPSCSLAIGYIRCRAIEKVLESIKIPCQNSTYGCKITMNLNQINEHETSC 120
           SSCCSKVHNKCPSCSLAIGYIRCRAIEKVLESIKIPCQNS YGCKITMNLNQINEHETSC
Sbjct: 61  SSCCSKVHNKCPSCSLAIGYIRCRAIEKVLESIKIPCQNSKYGCKITMNLNQINEHETSC 120

Query: 121 PYEPCSCPISDCSYVDSAEHLGSHFRKRHPDSAKSFLYNSRFTICLNSGDRYRIFQAEND 180
           PYEPCSCPISDCSYVDSAEHLGSHFRKRH DSAKSFLYNSRFTICLNSGDRYRIFQAEND
Sbjct: 121 PYEPCSCPISDCSYVDSAEHLGSHFRKRHQDSAKSFLYNSRFTICLNSGDRYRIFQAEND 180

Query: 181 DAVFVLSYSFEIFGNAVTLSRIGSPLCEKKFCYDIKAKSQGSVLSLQSVANEIRRWIEDP 240
           DA+FVLSYSFEIFGNAVTLSRIGSPL EKKFCYDIKAKSQGSVLSLQSVA EI+RW EDP
Sbjct: 181 DALFVLSYSFEIFGNAVTLSRIGSPLSEKKFCYDIKAKSQGSVLSLQSVAKEIQRWTEDP 240

Query: 241 PFKGSLLIPNEFFGSSSAQTILEIRIWPSR 271
           PFKGSLLIPNEFFGSSSAQTILEIRIWPSR
Sbjct: 241 PFKGSLLIPNEFFGSSSAQTILEIRIWPSR 270

BLAST of CmoCh01G003640 vs. NCBI nr
Match: XP_022997790.1 (E3 ubiquitin-protein ligase SINA-like 10 isoform X2 [Cucurbita maxima])

HSP 1 Score: 480.7 bits (1236), Expect = 8.2e-132
Identity = 230/270 (85.19%), Postives = 240/270 (88.89%), Query Frame = 0

Query: 1   MADSRSSDGEKERRRSVNCSRGKNEAMKVIFTDPQILDCYICCEPLSVPVYQCENGHISC 60
           MA SRSSDGEKERR SVN SR KNE MKVI TDPQ+LDCYICC PLSVPVYQCENGHISC
Sbjct: 1   MAGSRSSDGEKERRSSVNSSRWKNETMKVILTDPQVLDCYICCVPLSVPVYQCENGHISC 60

Query: 61  SSCCSKVHNKCPSCSLAIGYIRCRAIEKVLESIKIPCQNSTYGCKITMNLNQINEHETSC 120
           SSCCSKVHNKCPSCSL IGY+RCRAIEKVLESIK+PCQNS YGCK T NLNQINEHET C
Sbjct: 61  SSCCSKVHNKCPSCSLPIGYMRCRAIEKVLESIKMPCQNSRYGCKTTTNLNQINEHETLC 120

Query: 121 PYEPCSCPISDCSYVDSAEHLGSHFRKRHPDSAKSFLYNSRFTICLNSGDRYRIFQAEND 180
           PY+PCSCP+SDCSYVDSAEHLGSHFRKRH DSAKS  YNSRFTICLNS D+Y IFQAEND
Sbjct: 121 PYQPCSCPLSDCSYVDSAEHLGSHFRKRHQDSAKSISYNSRFTICLNSDDKYCIFQAEND 180

Query: 181 DAVFVLSYSFEIFGNAVTLSRIGSPLCEKKFCYDIKAKSQGSVLSLQSVANEIRRWIEDP 240
             +FVLSYSFE FG+AVTL RIG    EKKFCYDIKAKS G+VLSL SVA EI+RW EDP
Sbjct: 181 GVLFVLSYSFETFGDAVTLKRIGPRSSEKKFCYDIKAKSHGNVLSLLSVAKEIQRWTEDP 240

Query: 241 PFKGSLLIPNEFFGSSSAQTILEIRIWPSR 271
           P KGSLLIPNEFFGSSSAQTILEIRIWPSR
Sbjct: 241 PSKGSLLIPNEFFGSSSAQTILEIRIWPSR 270

BLAST of CmoCh01G003640 vs. NCBI nr
Match: KAG6606997.1 (E3 ubiquitin-protein ligase SINA-like 10, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 479.6 bits (1233), Expect = 1.8e-131
Identity = 230/270 (85.19%), Postives = 241/270 (89.26%), Query Frame = 0

Query: 1   MADSRSSDGEKERRRSVNCSRGKNEAMKVIFTDPQILDCYICCEPLSVPVYQCENGHISC 60
           MA SRSSDGEKERR S N SR KNE MKVI TDPQ+LDCYICCEPLSVPVYQCENGHISC
Sbjct: 1   MAGSRSSDGEKERRSSANSSRRKNETMKVILTDPQVLDCYICCEPLSVPVYQCENGHISC 60

Query: 61  SSCCSKVHNKCPSCSLAIGYIRCRAIEKVLESIKIPCQNSTYGCKITMNLNQINEHETSC 120
           SSCCSKV NKCPSCSLAIGY+RCRAIEKVLESIK+PCQNS YGCK T NLNQINEHET C
Sbjct: 61  SSCCSKVQNKCPSCSLAIGYMRCRAIEKVLESIKMPCQNSRYGCKTTTNLNQINEHETLC 120

Query: 121 PYEPCSCPISDCSYVDSAEHLGSHFRKRHPDSAKSFLYNSRFTICLNSGDRYRIFQAEND 180
           PY+PCSCP+SDCSYVDSAE+L SHFRKRH DSAKS  YNSRFTICLNS D+Y IFQAEND
Sbjct: 121 PYQPCSCPLSDCSYVDSAEYLSSHFRKRHQDSAKSISYNSRFTICLNSDDKYCIFQAEND 180

Query: 181 DAVFVLSYSFEIFGNAVTLSRIGSPLCEKKFCYDIKAKSQGSVLSLQSVANEIRRWIEDP 240
             +FVLSYSFE FG+AVTL+RIG    EKKFCYDIKAKSQGSVLSL SVA EI+RW EDP
Sbjct: 181 GVLFVLSYSFETFGDAVTLNRIGPRSSEKKFCYDIKAKSQGSVLSLLSVAKEIQRWTEDP 240

Query: 241 PFKGSLLIPNEFFGSSSAQTILEIRIWPSR 271
           P KGSLLIPNEFFGSSSAQTILEIRIWPSR
Sbjct: 241 PSKGSLLIPNEFFGSSSAQTILEIRIWPSR 270

BLAST of CmoCh01G003640 vs. TAIR 10
Match: AT5G37930.1 (Protein with RING/U-box and TRAF-like domains )

HSP 1 Score: 196.8 bits (499), Expect = 2.2e-50
Identity = 109/280 (38.93%), Postives = 152/280 (54.29%), Query Frame = 0

Query: 4   SRSSDGE---KERR-----RSVNCSRGKNEAMKVIFTDPQILDCYICCEPLSVPVYQCEN 63
           S  SD E   +ERR      S + S  K+  + V   DP +LDC ICCEPL +P++QC+N
Sbjct: 70  SDDSDREVVIEERRFGKFVNSQSSSSSKDSPLSVTLLDPDVLDCPICCEPLKIPIFQCDN 129

Query: 64  GHISCSSCCSKVHNKCPSCSLAIGYIRCRAIEKVLESIKIPCQNSTYGCKITMNLNQINE 123
           GH++C+ CC+KV N+CPSC+L IGY+RCRA+EKV+E+ ++ C N+ YGCK + +      
Sbjct: 130 GHLACTLCCTKVRNRCPSCTLPIGYVRCRAMEKVIEASRVSCLNAKYGCKESTSYGNRFS 189

Query: 124 HETSCPYEPCSCPISDCSYVDSAEHLGSHFRKRHPDSAKSFLYNSRFTICLNSGDRYRIF 183
           HE  C + PCSCPI DC Y    + L +H R  H D   SF++N+R TI L+   +  I 
Sbjct: 190 HEQVCVFTPCSCPILDCHYTGYYKDLNNHVRAEHKDDLISFVWNTRLTISLDLNKKTTIL 249

Query: 184 QAENDDAVFVLSYSFEIFGNAVTLS-------RIGSPLCE-KKFCYDIKAKSQGSVLSLQ 243
           Q END  V V+     +    V++S        +G   C   K   D   K    V ++Q
Sbjct: 250 QEENDGHVIVVQVFRALHAVYVSVSCIAPLTPGVGRLSCRLAKITVDSLLKQGFMVKNIQ 309

Query: 244 SVANEIRRWIEDPPFKGSLLIPNEFFGSSSAQTILEIRIW 268
            V NE        P  G +LIP+  F   S    L ++IW
Sbjct: 310 KVTNE-------HPEDGFMLIPSYLF---SGNDNLNLQIW 339

BLAST of CmoCh01G003640 vs. TAIR 10
Match: AT5G37890.1 (Protein with RING/U-box and TRAF-like domains )

HSP 1 Score: 154.1 bits (388), Expect = 1.6e-37
Identity = 87/269 (32.34%), Postives = 144/269 (53.53%), Query Frame = 0

Query: 4   SRSSDGEKERRRSVNCSRGKNEAMKVIFTDPQILDCYICCEPLSVPVYQCENGHISCSSC 63
           S S   +K +  S + ++   +    +  D +ILDC IC E  ++P++QC+NGH++CSSC
Sbjct: 16  SNSILSQKRQLSSSDAAKRDAKKRSTMLMDLEILDCPICYEAFTIPIFQCDNGHLACSSC 75

Query: 64  CSKVHNKCPSCSLAIGYIRCRAIEKVLESIKIPCQNSTYGCKITMNLNQINEHETSCPYE 123
           C K++NKCP+C+  +G+ RCRA+E VLESI IPC N+  GCK  ++  +   HE  C + 
Sbjct: 76  CPKLNNKCPACTSPVGHNRCRAMESVLESILIPCPNAKLGCKKNVSYGKELTHEKECMFS 135

Query: 124 PCSCPISDCSYVDSAEHLGSHFRKRHPD--SAKSFLYNSRFTICLNSGDRYRIFQAENDD 183
            C+CP  DC+Y  S + L +H+R  H +     +F+ +   ++ +N   +  I      +
Sbjct: 136 HCACPALDCNYTSSYKDLYTHYRITHMEINQINTFICDIPLSVRMNISKKILIRTEHLTN 195

Query: 184 AVFVLSYSFEIFGNAVTLSRIGSPLCE-KKFCYDIKAKSQGSVLSLQSVANEIRRWIE-- 243
            +F +    E +G  VT+S I     E  ++ Y +     G  +  QS   E++R ++  
Sbjct: 196 HLFAVQCFREPYGVYVTVSCIAPSSPELSQYSYALSYTVDGHTVIYQS--PEVKRVLKLS 255

Query: 244 -DPPFKGSLLIPNEFFGSSSAQTILEIRI 267
              P +  +LIPN          +LE+RI
Sbjct: 256 FQTPQENFMLIPNSLLRGD----VLEMRI 278

BLAST of CmoCh01G003640 vs. TAIR 10
Match: AT5G62800.1 (Protein with RING/U-box and TRAF-like domains )

HSP 1 Score: 150.6 bits (379), Expect = 1.8e-36
Identity = 91/270 (33.70%), Postives = 137/270 (50.74%), Query Frame = 0

Query: 1   MADSRS-SDGEKERRRSVN---CSRGKNEAMKVIFTDPQILDCYICCEPLSVPVYQCENG 60
           M DS S    +  +R+S +     R +NE       D  +LDC +C EPL++P +QC++G
Sbjct: 1   MEDSNSHPQNQTSKRKSSHPQKKQRMENETRSAKLLDLDVLDCPVCFEPLTIPTFQCDDG 60

Query: 61  HISCSSCCSKVHNKC--PSCSLAIGYIRCRAIEKVLESIKIPCQNSTYGCKITMNLNQIN 120
           HI C+ C +KV NKC  P C L IG  RC A+E+VLES  +PCQN+ +GC  +++  +++
Sbjct: 61  HIVCNFCFAKVSNKCPGPGCDLPIGNKRCFAMERVLESAFVPCQNTEFGCTKSVSYEKVS 120

Query: 121 EHETSCPYEPCSCPISDCSYVDSAEHLGSHFRKRHPDSAKSFLYNSRFT----------I 180
            HE  C Y  CSCP  +C+Y  S   +  HF +RH       LYNS             +
Sbjct: 121 SHEKECNYSQCSCPNLECNYTGSYNIIYGHFMRRH-------LYNSTIVSSKWGYSTVDV 180

Query: 181 CLNSGDRYRIFQAENDDAVFVLSYSFEIFGNAVTLSRIGSPLCE-KKFCYDIKAKSQGSV 240
            +N  ++  +        +FV+    E  G  VT+ RI  P  E KKF Y +     G  
Sbjct: 181 LINIKEKVSVLWESRQKLLFVVQCFKERHGVYVTVRRIAPPASEFKKFSYRLSYSIDGHN 240

Query: 241 LSLQSVANEIRRWIE---DPPFKGSLLIPN 251
           ++ +S   E++R +E     P    + +PN
Sbjct: 241 VTYES--PEVKRLLEVNSQIPDDSFMFVPN 261

BLAST of CmoCh01G003640 vs. TAIR 10
Match: AT5G37870.1 (Protein with RING/U-box and TRAF-like domains )

HSP 1 Score: 150.6 bits (379), Expect = 1.8e-36
Identity = 84/255 (32.94%), Postives = 136/255 (53.33%), Query Frame = 0

Query: 4   SRSSDGEKERRRSVNCSRGKNEAMK---VIFTDPQILDCYICCEPLSVPVYQCENGHISC 63
           S  +  +K    S++   G+    K    + TD  ILDC IC + L +PV+QC NGH++C
Sbjct: 7   SERNGSQKRHCSSISSDDGRKRVDKTRSAMLTDLDILDCPICYQALKIPVFQCGNGHLAC 66

Query: 64  SSCCSKVHNKCPSCSLAIGYIRCRAIEKVLESIKIPCQNSTYGCKITMNLNQINEHETSC 123
           SSCC K+ NKCP+C+L +G+IRCRA+E+VLES+ +PC+ +  GC  T+   + + HE  C
Sbjct: 67  SSCCPKLRNKCPACALPVGHIRCRAMERVLESVLVPCRYADLGCTKTIYYGRESTHEKIC 126

Query: 124 PYEPCSCPISDCSYVDSAEHLGSHFRKRHPDSAKSFLYN--SRFTICLNSGDRYRIFQAE 183
            + PCSCP+  C+Y  S + L  H+   H   + ++ +N  S     +   D+  I +  
Sbjct: 127 NFSPCSCPVQGCNYTGSYKDLYEHYDLTHSTGSTAYSFNGVSYIAAMMFISDKILIERVY 186

Query: 184 NDDAVFVLSYSFEIFGNAVTLSRIGSPLCE-KKFCYDIKAKS-QGSVLSLQS-VANEIRR 243
               +FV+    E  G  V++S I     E  +F Y +   + +G  ++ QS    ++ +
Sbjct: 187 EKKLLFVVQCFEEPCGVYVSVSCIAPSAPEVGEFSYGLLYTTWEGVTMTYQSPKVKKVLK 246

Query: 244 WIEDPPFKGSLLIPN 251
                P    +LIP+
Sbjct: 247 VSSQRPKDSFMLIPH 261

BLAST of CmoCh01G003640 vs. TAIR 10
Match: AT5G37910.1 (Protein with RING/U-box and TRAF-like domains )

HSP 1 Score: 148.7 bits (374), Expect = 6.9e-36
Identity = 81/236 (34.32%), Postives = 125/236 (52.97%), Query Frame = 0

Query: 23  KNEAMKVIFTDPQILDCYICCEPLSVPVYQCENGHISCSSCCSKVHNKCPSCSLAIGYIR 82
           K +   ++  D  ILDC ICCE L+ P++QC+NGH++C SCC K+ NKCP+C+L +G+ R
Sbjct: 22  KRQRSAIVLLDLDILDCPICCEALTSPIFQCDNGHLACGSCCPKLSNKCPACTLPVGHSR 81

Query: 83  CRAIEKVLESIKIPCQNSTYGCKITMNLNQINEHETSCPYEPCSCPISDCSYVDSAEHLG 142
            RA+E VLESI IPC N  +GC  +    + + HE  C +  CSCP S C Y  S + L 
Sbjct: 82  SRAMESVLESILIPCPNVRFGCTKSFFYGKESAHEKECIFSQCSCPSSVCDYTGSYKDLY 141

Query: 143 SHFRKRHPD----SAKSFLYNSRFTICLNSGDRYRIFQAENDDAVFVLSYSFEIFGNAVT 202
           +H++  H      + K F   + FT  +   D+  I +      +  +    E  G  VT
Sbjct: 142 AHYKLTHSTNIFWNIKRFRCANFFTTSMLISDKILIKRVHEKKLLLAVQCFREPCGVYVT 201

Query: 203 LSRIGSPLCE-KKFCYDIKAKSQGSVLSLQSVANEIRRWIE---DPPFKGSLLIPN 251
           +S I     E  +F Y +     G  ++ +S   E++R  +   + P +  +LIP+
Sbjct: 202 VSFIAPSAPEVGEFSYQLSYNVDGHTVTYES--PEVKRVCKVSIETPQENFMLIPH 255

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q84K343.1e-4938.93E3 ubiquitin-protein ligase SINA-like 10 OS=Arabidopsis thaliana OX=3702 GN=At5g... [more]
Q9FKD72.3e-3632.34E3 ubiquitin-protein ligase SINA-like 7 OS=Arabidopsis thaliana OX=3702 GN=At5g3... [more]
Q9FM142.5e-3533.70E3 ubiquitin-protein ligase SINA-like 11 OS=Arabidopsis thaliana OX=3702 GN=At5g... [more]
Q9FKD92.5e-3532.94Putative E3 ubiquitin-protein ligase SINA-like 6 OS=Arabidopsis thaliana OX=3702... [more]
Q9FKD59.7e-3534.32Putative E3 ubiquitin-protein ligase SINA-like 9 OS=Arabidopsis thaliana OX=3702... [more]
Match NameE-valueIdentityDescription
A0A6J1GBP17.2e-158100.00RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111452508... [more]
A0A6J1K8G55.3e-15397.41RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111492642 P... [more]
A0A6J1KAW24.0e-13285.19RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111492641 P... [more]
A0A6J1KCJ82.3e-12776.67RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111492641 P... [more]
A0A6J1CNI11.7e-11173.23RING-type E3 ubiquitin transferase OS=Momordica charantia OX=3673 GN=LOC11101322... [more]
Match NameE-valueIdentityDescription
XP_022949045.11.5e-157100.00uncharacterized protein LOC111452508 [Cucurbita moschata][more]
KAG6606996.12.8e-15699.26E3 ubiquitin-protein ligase SINA-like 10, partial [Cucurbita argyrosperma subsp.... [more]
XP_022997791.11.1e-15297.41E3 ubiquitin-protein ligase SINA-like 10 [Cucurbita maxima][more]
XP_022997790.18.2e-13285.19E3 ubiquitin-protein ligase SINA-like 10 isoform X2 [Cucurbita maxima][more]
KAG6606997.11.8e-13185.19E3 ubiquitin-protein ligase SINA-like 10, partial [Cucurbita argyrosperma subsp.... [more]
Match NameE-valueIdentityDescription
AT5G37930.12.2e-5038.93Protein with RING/U-box and TRAF-like domains [more]
AT5G37890.11.6e-3732.34Protein with RING/U-box and TRAF-like domains [more]
AT5G62800.11.8e-3633.70Protein with RING/U-box and TRAF-like domains [more]
AT5G37870.11.8e-3632.94Protein with RING/U-box and TRAF-like domains [more]
AT5G37910.16.9e-3634.32Protein with RING/U-box and TRAF-like domains [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR018121Seven-in-absentia protein, TRAF-like domainPFAMPF03145Sinacoord: 81..238
e-value: 9.2E-18
score: 64.9
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 87..145
e-value: 1.6E-16
score: 62.3
NoneNo IPR availablePANTHERPTHR46632:SF16E3 UBIQUITIN-PROTEIN LIGASE SINA-LIKE 4coord: 16..268
NoneNo IPR availableSUPERFAMILY49599TRAF domain-likecoord: 92..254
IPR044286E3 ubiquitin-protein ligase SINA-like, plantPANTHERPTHR46632E3 UBIQUITIN-PROTEIN LIGASE SINA-LIKE 4coord: 16..268
IPR013010Zinc finger, SIAH-typePROSITEPS51081ZF_SIAHcoord: 92..150
score: 13.579697

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh01G003640.1CmoCh01G003640.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0007275 multicellular organism development
biological_process GO:0016567 protein ubiquitination
biological_process GO:0006511 ubiquitin-dependent protein catabolic process
cellular_component GO:0005737 cytoplasm
molecular_function GO:0016740 transferase activity
molecular_function GO:0008270 zinc ion binding