CmoCh01G003500 (gene) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh01G003500
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
Descriptionaspartic proteinase PCS1-like
LocationCmo_Chr01: 1692465 .. 1693523 (+)
RNA-Seq ExpressionCmoCh01G003500
SyntenyCmoCh01G003500
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGAACGCCGCCGCAGCTGATGGACATGGTCCTAGACACCGGCAGCCAACTCTCTTGGATTCAATGCCACGGCACAGTTAACGGAAAATCAGTTAAGCCCAGATTCAAGTGGTTTAACCCTTCTCTCTCCTCCACTTTCTCTTTCCTCCCCTGTAACAGCTCTCTCTGCAAACCCCGAATTCCCGATTTTACCCTTCCCACTTCCTGTGACCCACGTCGCCACTGCCACTACTCCTACTTCTACGCCGATGGGACCTTGGCCGAGGGTAATCTCGTCACTGAAAAATTCTCCTTCACCGCCTCTCCCATCGCTATCGGCTGCGTTAAGCCCTCCGCCGAAAACAGGGGTATTTTGGGAATGAACACCGGACACCTCTCCTTCATCTCTCAGGCCAAAATATCCAAGTTTTCCTATTGTGTCCCGGGTCGAAGCAGGTCGGATCTAACCGGGTTGTTCTACCTTGGAGACAACCCGAATTCGGGTAAATTCAAATATGTCAACATGTTGACTTTTCCCGAAAGTCAAAACTCCCCGAATCTTGACAAACTGGCCTACACCCTCCCAATGAAGGGCATAAGAATCGGCGCAGTCCAACTCAAAATCTCTCCGGCCGTTTTTAAACCGGACCCAACTGGGTCTGGTCAGACAATGATTGACTCCGGCTCGGAGTTGACTTACTTGGTAGATGAAGCTTACAACAATGTTAGAGCAGAGATCGTGAGATTAGTGGGGCCCATGATGAAGAAAGGATATGAATACGCCTCCGTCGCCGATATGTGTTTCGACGGTGCAATGGCGGCGGCGGCAGGGCGGAGGATTGGTGAGATGTGGTTTCAGTTTGAGAATGGAGTGGAGATATTGGTCGGGAAAGGGGAAGGGTTATTGACGGTAGTGGAAAAAGGGGTGAAGTGTGTGGGGATCGGACGGTCAGGTAGGCTCGGGACTGAGAGTAATATGATCGGAAATGTTCATCAGCAGAATATGTGGGTGGAGTACGATTTGGCCAATAAGAGAGTTGGGTTTGGTGGAGCTGTGTGTAGTGGATTGAAGGCATGA

mRNA sequence

ATGGGAACGCCGCCGCAGCTGATGGACATGGTCCTAGACACCGGCAGCCAACTCTCTTGGATTCAATGCCACGGCACAGTTAACGGAAAATCAGTTAAGCCCAGATTCAAGTGGTTTAACCCTTCTCTCTCCTCCACTTTCTCTTTCCTCCCCTGTAACAGCTCTCTCTGCAAACCCCGAATTCCCGATTTTACCCTTCCCACTTCCTGTGACCCACGTCGCCACTGCCACTACTCCTACTTCTACGCCGATGGGACCTTGGCCGAGGGTAATCTCGTCACTGAAAAATTCTCCTTCACCGCCTCTCCCATCGCTATCGGCTGCGTTAAGCCCTCCGCCGAAAACAGGGGTATTTTGGGAATGAACACCGGACACCTCTCCTTCATCTCTCAGGCCAAAATATCCAAGTTTTCCTATTGTGTCCCGGGTCGAAGCAGGTCGGATCTAACCGGGTTGTTCTACCTTGGAGACAACCCGAATTCGGGTAAATTCAAATATGTCAACATGTTGACTTTTCCCGAAAGTCAAAACTCCCCGAATCTTGACAAACTGGCCTACACCCTCCCAATGAAGGGCATAAGAATCGGCGCAGTCCAACTCAAAATCTCTCCGGCCGTTTTTAAACCGGACCCAACTGGGTCTGGTCAGACAATGATTGACTCCGGCTCGGAGTTGACTTACTTGGTAGATGAAGCTTACAACAATGTTAGAGCAGAGATCGTGAGATTAGTGGGGCCCATGATGAAGAAAGGATATGAATACGCCTCCGTCGCCGATATGTGTTTCGACGGTGCAATGGCGGCGGCGGCAGGGCGGAGGATTGGTGAGATGTGGTTTCAGTTTGAGAATGGAGTGGAGATATTGGTCGGGAAAGGGGAAGGGTTATTGACGGTAGTGGAAAAAGGGGTGAAGTGTGTGGGGATCGGACGGTCAGGTAGGCTCGGGACTGAGAGTAATATGATCGGAAATGTTCATCAGCAGAATATGTGGGTGGAGTACGATTTGGCCAATAAGAGAGTTGGGTTTGGTGGAGCTGTGTGTAGTGGATTGAAGGCATGA

Coding sequence (CDS)

ATGGGAACGCCGCCGCAGCTGATGGACATGGTCCTAGACACCGGCAGCCAACTCTCTTGGATTCAATGCCACGGCACAGTTAACGGAAAATCAGTTAAGCCCAGATTCAAGTGGTTTAACCCTTCTCTCTCCTCCACTTTCTCTTTCCTCCCCTGTAACAGCTCTCTCTGCAAACCCCGAATTCCCGATTTTACCCTTCCCACTTCCTGTGACCCACGTCGCCACTGCCACTACTCCTACTTCTACGCCGATGGGACCTTGGCCGAGGGTAATCTCGTCACTGAAAAATTCTCCTTCACCGCCTCTCCCATCGCTATCGGCTGCGTTAAGCCCTCCGCCGAAAACAGGGGTATTTTGGGAATGAACACCGGACACCTCTCCTTCATCTCTCAGGCCAAAATATCCAAGTTTTCCTATTGTGTCCCGGGTCGAAGCAGGTCGGATCTAACCGGGTTGTTCTACCTTGGAGACAACCCGAATTCGGGTAAATTCAAATATGTCAACATGTTGACTTTTCCCGAAAGTCAAAACTCCCCGAATCTTGACAAACTGGCCTACACCCTCCCAATGAAGGGCATAAGAATCGGCGCAGTCCAACTCAAAATCTCTCCGGCCGTTTTTAAACCGGACCCAACTGGGTCTGGTCAGACAATGATTGACTCCGGCTCGGAGTTGACTTACTTGGTAGATGAAGCTTACAACAATGTTAGAGCAGAGATCGTGAGATTAGTGGGGCCCATGATGAAGAAAGGATATGAATACGCCTCCGTCGCCGATATGTGTTTCGACGGTGCAATGGCGGCGGCGGCAGGGCGGAGGATTGGTGAGATGTGGTTTCAGTTTGAGAATGGAGTGGAGATATTGGTCGGGAAAGGGGAAGGGTTATTGACGGTAGTGGAAAAAGGGGTGAAGTGTGTGGGGATCGGACGGTCAGGTAGGCTCGGGACTGAGAGTAATATGATCGGAAATGTTCATCAGCAGAATATGTGGGTGGAGTACGATTTGGCCAATAAGAGAGTTGGGTTTGGTGGAGCTGTGTGTAGTGGATTGAAGGCATGA

Protein sequence

MGTPPQLMDMVLDTGSQLSWIQCHGTVNGKSVKPRFKWFNPSLSSTFSFLPCNSSLCKPRIPDFTLPTSCDPRRHCHYSYFYADGTLAEGNLVTEKFSFTASPIAIGCVKPSAENRGILGMNTGHLSFISQAKISKFSYCVPGRSRSDLTGLFYLGDNPNSGKFKYVNMLTFPESQNSPNLDKLAYTLPMKGIRIGAVQLKISPAVFKPDPTGSGQTMIDSGSELTYLVDEAYNNVRAEIVRLVGPMMKKGYEYASVADMCFDGAMAAAAGRRIGEMWFQFENGVEILVGKGEGLLTVVEKGVKCVGIGRSGRLGTESNMIGNVHQQNMWVEYDLANKRVGFGGAVCSGLKA
Homology
BLAST of CmoCh01G003500 vs. ExPASy Swiss-Prot
Match: Q9LZL3 (Aspartic proteinase PCS1 OS=Arabidopsis thaliana OX=3702 GN=PCS1 PE=2 SV=1)

HSP 1 Score: 221.9 bits (564), Expect = 1.2e-56
Identity = 133/376 (35.37%), Postives = 197/376 (52.39%), Query Frame = 0

Query: 1   MGTPPQLMDMVLDTGSQLSWIQCHGTVNGKSVKPRFKWFNPSLSSTFSFLPCNSSLCKPR 60
           +GTPPQ + MV+DTGS+LSW++C+ + N   V      F+P+ SS++S +PC+S  C+ R
Sbjct: 79  VGTPPQNISMVIDTGSELSWLRCNRSSNPNPVNN----FDPTRSSSYSPIPCSSPTCRTR 138

Query: 61  IPDFTLPTSCDPRRHCHYSYFYADGTLAEGNLVTEKFSF----TASPIAIGCV------- 120
             DF +P SCD  + CH +  YAD + +EGNL  E F F      S +  GC+       
Sbjct: 139 TRDFLIPASCDSDKLCHATLSYADASSSEGNLAAEIFHFGNSTNDSNLIFGCMGSVSGSD 198

Query: 121 -KPSAENRGILGMNTGHLSFISQAKISKFSYCVPGRSRSDLTGLFYLGDNPNSGKFKYVN 180
            +   +  G+LGMN G LSFISQ    KFSYC+ G    D  G   LGD+     F ++ 
Sbjct: 199 PEEDTKTTGLLGMNRGSLSFISQMGFPKFSYCISG--TDDFPGFLLLGDS----NFTWLT 258

Query: 181 MLTFPE----SQNSPNLDKLAYTLPMKGIRIGAVQLKISPAVFKPDPTGSGQTMIDSGSE 240
            L +      S   P  D++AYT+ + GI++    L I  +V  PD TG+GQTM+DSG++
Sbjct: 259 PLNYTPLIRISTPLPYFDRVAYTVQLTGIKVNGKLLPIPKSVLVPDHTGAGQTMVDSGTQ 318

Query: 241 LTYLVDEAYNNVRAEIVRLVGPMM----KKGYEYASVADMCF---DGAMAAAAGRRIGEM 300
            T+L+   Y  +R+  +     ++       + +    D+C+      + +    R+  +
Sbjct: 319 FTFLLGPVYTALRSHFLNRTNGILTVYEDPDFVFQGTMDLCYRISPVRIRSGILHRLPTV 378

Query: 301 WFQFENGVEILVGKGEGL------LTVVEKGVKCVGIGRSGRLGTESNMIGNVHQQNMWV 348
              FE G EI V  G+ L      LTV    V C   G S  +G E+ +IG+ HQQNMW+
Sbjct: 379 SLVFE-GAEIAV-SGQPLLYRVPHLTVGNDSVYCFTFGNSDLMGMEAYVIGHHHQQNMWI 438

BLAST of CmoCh01G003500 vs. ExPASy Swiss-Prot
Match: Q766C3 (Aspartic proteinase nepenthesin-1 OS=Nepenthes gracilis OX=150966 GN=nep1 PE=1 SV=1)

HSP 1 Score: 151.8 bits (382), Expect = 1.5e-35
Identity = 112/356 (31.46%), Postives = 165/356 (46.35%), Query Frame = 0

Query: 1   MGTPPQLMDMVLDTGSQLSWIQCHGTVNGKSVKPRFKWFNPSLSSTFSFLPCNSSLCKPR 60
           +GTP Q    ++DTGS L W QC      +        FNP  SS+FS LPC+S LC+  
Sbjct: 101 IGTPAQPFSAIMDTGSDLIWTQCQPCT--QCFNQSTPIFNPQGSSSFSTLPCSSQLCQ-- 160

Query: 61  IPDFTLPTSCDPRRHCHYSYFYADGTLAEGNLVTEKFSF---TASPIAIGCVKPS----- 120
                L +       C Y+Y Y DG+  +G++ TE  +F   +   I  GC + +     
Sbjct: 161 ----ALSSPTCSNNFCQYTYGYGDGSETQGSMGTETLTFGSVSIPNITFGCGENNQGFGQ 220

Query: 121 AENRGILGMNTGHLSFISQAKISKFSYCVPGRSRSDLTGLFYLGDNPNSGKFKYVNMLTF 180
               G++GM  G LS  SQ  ++KFSYC+     S  + L  LG   NS      N  T 
Sbjct: 221 GNGAGLVGMGRGPLSLPSQLDVTKFSYCMTPIGSSTPSNLL-LGSLANSVTAGSPN-TTL 280

Query: 181 PESQNSPNLDKLAYTLPMKGIRIGAVQLKISPAVFKPDP-TGSGQTMIDSGSELTYLVDE 240
            +S   P      Y + + G+ +G+ +L I P+ F  +   G+G  +IDSG+ LTY V+ 
Sbjct: 281 IQSSQIPTF----YYITLNGLSVGSTRLPIDPSAFALNSNNGTGGIIIDSGTTLTYFVNN 340

Query: 241 AYNNVRAEIVRLVGPMMKKGYEYASVADMCFDGAMAAAAGRRIGEMWFQFENGVEILVGK 300
           AY +VR E +  +   +  G   +S  D+CF    +  +  +I      F+ G   L   
Sbjct: 341 AYQSVRQEFISQINLPVVNG--SSSGFDLCFQ-TPSDPSNLQIPTFVMHFDGG--DLELP 400

Query: 301 GEGLLTVVEKGVKCVGIGRSGRLGTESNMIGNVHQQNMWVEYDLANKRVGFGGAVC 348
            E        G+ C+ +G S +     ++ GN+ QQNM V YD  N  V F  A C
Sbjct: 401 SENYFISPSNGLICLAMGSSSQ---GMSIFGNIQQQNMLVVYDTGNSVVSFASAQC 434

BLAST of CmoCh01G003500 vs. ExPASy Swiss-Prot
Match: Q766C2 (Aspartic proteinase nepenthesin-2 OS=Nepenthes gracilis OX=150966 GN=nep2 PE=1 SV=1)

HSP 1 Score: 151.0 bits (380), Expect = 2.5e-35
Identity = 117/359 (32.59%), Postives = 168/359 (46.80%), Query Frame = 0

Query: 1   MGTPPQLMDMVLDTGSQLSWIQCHGTVNGKSVKPRFKWFNPSLSSTFSFLPCNSSLCKPR 60
           +GTP      ++DTGS L W QC       S       FNP  SS+FS LPC S  C+  
Sbjct: 102 IGTPDSSFSAIMDTGSDLIWTQCEPCTQCFSQPTPI--FNPQDSSSFSTLPCESQYCQ-- 161

Query: 61  IPDFTLPTSCDPRRHCHYSYFYADGTLAEGNLVTEKFSFTASP---IAIGCVKPS----- 120
                LP+       C Y+Y Y DG+  +G + TE F+F  S    IA GC + +     
Sbjct: 162 ----DLPSETCNNNECQYTYGYGDGSTTQGYMATETFTFETSSVPNIAFGCGEDNQGFGQ 221

Query: 121 AENRGILGMNTGHLSFISQAKISKFSYCVPGRSRSDLTGLFYLGDNPNSGKFKYVNMLTF 180
               G++GM  G LS  SQ  + +FSYC+     S  + L  LG +  SG  +     T 
Sbjct: 222 GNGAGLIGMGWGPLSLPSQLGVGQFSYCMTSYGSSSPSTL-ALG-SAASGVPEGSPSTTL 281

Query: 181 PESQNSPNLDKLAYTLPMKGIRIGAVQLKISPAVFKPDPTGSGQTMIDSGSELTYLVDEA 240
             S  +P      Y + ++GI +G   L I  + F+    G+G  +IDSG+ LTYL  +A
Sbjct: 282 IHSSLNPTY----YYITLQGITVGGDNLGIPSSTFQLQDDGTGGMIIDSGTTLTYLPQDA 341

Query: 241 YNNVRAEIVRLVGPMMKKGYEYASVADMCF----DGAMAAAAGRRIGEMWFQFENGVEIL 300
           YN V       +   +    E +S    CF    DG+       ++ E+  QF+ GV + 
Sbjct: 342 YNAVAQAFTDQIN--LPTVDESSSGLSTCFQQPSDGSTV-----QVPEISMQFDGGV-LN 401

Query: 301 VGKGEGLLTVVEKGVKCVGIGRSGRLGTESNMIGNVHQQNMWVEYDLANKRVGFGGAVC 348
           +G+   L++  E GV C+ +G S +LG   ++ GN+ QQ   V YDL N  V F    C
Sbjct: 402 LGEQNILISPAE-GVICLAMGSSSQLGI--SIFGNIQQQETQVLYDLQNLAVSFVPTQC 435

BLAST of CmoCh01G003500 vs. ExPASy Swiss-Prot
Match: Q9LS40 (Protein ASPARTIC PROTEASE IN GUARD CELL 1 OS=Arabidopsis thaliana OX=3702 GN=ASPG1 PE=1 SV=1)

HSP 1 Score: 142.5 bits (358), Expect = 9.0e-33
Identity = 102/355 (28.73%), Postives = 165/355 (46.48%), Query Frame = 0

Query: 1   MGTPPQLMDMVLDTGSQLSWIQCHGTVNGKSVKPRFKWFNPSLSSTFSFLPCNSSLCKPR 60
           +GTP + M +VLDTGS ++WIQC    +    +     FNP+ SST+  L C++  C   
Sbjct: 168 VGTPAKEMYLVLDTGSDVNWIQCEPCAD--CYQQSDPVFNPTSSSTYKSLTCSAPQCS-- 227

Query: 61  IPDFTLPTSCDPRRHCHYSYFYADGTLAEGNLVTEKFSFTAS----PIAIGCVKPS---- 120
                L TS      C Y   Y DG+   G L T+  +F  S     +A+GC   +    
Sbjct: 228 ----LLETSACRSNKCLYQVSYGDGSFTVGELATDTVTFGNSGKINNVALGCGHDNEGLF 287

Query: 121 AENRGILGMNTGHLSFISQAKISKFSYCVPGRSRSDLTGLFYLGDNPNSGKFKYVNMLTF 180
               G+LG+  G LS  +Q K + FSYC+  R     + L +       G        T 
Sbjct: 288 TGAAGLLGLGGGVLSITNQMKATSFSYCLVDRDSGKSSSLDFNSVQLGGGD------ATA 347

Query: 181 PESQNSPNLDKLAYTLPMKGIRIGAVQLKISPAVFKPDPTGSGQTMIDSGSELTYLVDEA 240
           P  +N   +D   Y + + G  +G  ++ +  A+F  D +GSG  ++D G+ +T L  +A
Sbjct: 348 PLLRNK-KIDTF-YYVGLSGFSVGGEKVVLPDAIFDVDASGSGGVILDCGTAVTRLQTQA 407

Query: 241 YNNVRAEIVRLVGPMMKKGYEYASVADMCFDGAMAAAAGRRIGEMWFQFENGVEILVGKG 300
           YN++R   ++L    +KKG    S+ D C+D   ++ +  ++  + F F  G  + +   
Sbjct: 408 YNSLRDAFLKLT-VNLKKGSSSISLFDTCYD--FSSLSTVKVPTVAFHFTGGKSLDLPAK 467

Query: 301 EGLLTVVEKGVKCVGIGRSGRLGTESNMIGNVHQQNMWVEYDLANKRVGFGGAVC 348
             L+ V + G  C     +    +  ++IGNV QQ   + YDL+   +G  G  C
Sbjct: 468 NYLIPVDDSGTFCFAFAPT---SSSLSIIGNVQQQGTRITYDLSKNVIGLSGNKC 500

BLAST of CmoCh01G003500 vs. ExPASy Swiss-Prot
Match: Q9LNJ3 (Aspartyl protease family protein 2 OS=Arabidopsis thaliana OX=3702 GN=APF2 PE=2 SV=1)

HSP 1 Score: 134.4 bits (337), Expect = 2.5e-30
Identity = 109/360 (30.28%), Postives = 174/360 (48.33%), Query Frame = 0

Query: 1   MGTPPQLMDMVLDTGSQLSWIQCHGTVNGKSVKPRFKWFNPSLSSTFSFLPCNSSLCKPR 60
           +GTP + + MVLDTGS + W+QC       S       F+P  S T++ +PC+S  C+ R
Sbjct: 148 VGTPARYVYMVLDTGSDIVWLQCAPCRRCYSQSDPI--FDPRKSKTYATIPCSSPHCR-R 207

Query: 61  IPDFTLPTSCDPRRH-CHYSYFYADGTLAEGNLVTEKFSF---TASPIAIGCVKPS---- 120
           +        C+ RR  C Y   Y DG+   G+  TE  +F       +A+GC   +    
Sbjct: 208 LDS----AGCNTRRKTCLYQVSYGDGSFTVGDFSTETLTFRRNRVKGVALGCGHDNEGLF 267

Query: 121 AENRGILGMNTGHLSFISQAK---ISKFSYCVPGRSRSDLTGLFYLGDNPNSGKFKYVNM 180
               G+LG+  G LSF  Q       KFSYC+  RS S        G+   S   ++  +
Sbjct: 268 VGAAGLLGLGKGKLSFPGQTGHRFNQKFSYCLVDRSASSKPSSVVFGNAAVSRIARFTPL 327

Query: 181 LTFPESQNSPNLDKLAYTLPMKGIRIGAVQLK-ISPAVFKPDPTGSGQTMIDSGSELTYL 240
           L      ++P LD   Y + + GI +G  ++  ++ ++FK D  G+G  +IDSG+ +T L
Sbjct: 328 L------SNPKLDTF-YYVGLLGISVGGTRVPGVTASLFKLDQIGNGGVIIDSGTSVTRL 387

Query: 241 VDEAYNNVRAEIVRLVGPMMKKGYEYASVADMCFDGAMAAAAGRRIGEMWFQFENGVEIL 300
           +  AY  +R +  R+    +K+  ++ S+ D CFD  ++     ++  +   F  G ++ 
Sbjct: 388 IRPAYIAMR-DAFRVGAKTLKRAPDF-SLFDTCFD--LSNMNEVKVPTVVLHF-RGADVS 447

Query: 301 VGKGEGLLTVVEKGVKCVGIGRSGRLGTESNMIGNVHQQNMWVEYDLANKRVGFGGAVCS 349
           +     L+ V   G  C     +G +G  S +IGN+ QQ   V YDLA+ RVGF    C+
Sbjct: 448 LPATNYLIPVDTNGKFCFAF--AGTMGGLS-IIGNIQQQGFRVVYDLASSRVGFAPGGCA 485

BLAST of CmoCh01G003500 vs. ExPASy TrEMBL
Match: A0A6J1GAZ2 (aspartic proteinase PCS1-like OS=Cucurbita moschata OX=3662 GN=LOC111452506 PE=3 SV=1)

HSP 1 Score: 730.3 bits (1884), Expect = 3.7e-207
Identity = 352/352 (100.00%), Postives = 352/352 (100.00%), Query Frame = 0

Query: 1   MGTPPQLMDMVLDTGSQLSWIQCHGTVNGKSVKPRFKWFNPSLSSTFSFLPCNSSLCKPR 60
           MGTPPQLMDMVLDTGSQLSWIQCHGTVNGKSVKPRFKWFNPSLSSTFSFLPCNSSLCKPR
Sbjct: 1   MGTPPQLMDMVLDTGSQLSWIQCHGTVNGKSVKPRFKWFNPSLSSTFSFLPCNSSLCKPR 60

Query: 61  IPDFTLPTSCDPRRHCHYSYFYADGTLAEGNLVTEKFSFTASPIAIGCVKPSAENRGILG 120
           IPDFTLPTSCDPRRHCHYSYFYADGTLAEGNLVTEKFSFTASPIAIGCVKPSAENRGILG
Sbjct: 61  IPDFTLPTSCDPRRHCHYSYFYADGTLAEGNLVTEKFSFTASPIAIGCVKPSAENRGILG 120

Query: 121 MNTGHLSFISQAKISKFSYCVPGRSRSDLTGLFYLGDNPNSGKFKYVNMLTFPESQNSPN 180
           MNTGHLSFISQAKISKFSYCVPGRSRSDLTGLFYLGDNPNSGKFKYVNMLTFPESQNSPN
Sbjct: 121 MNTGHLSFISQAKISKFSYCVPGRSRSDLTGLFYLGDNPNSGKFKYVNMLTFPESQNSPN 180

Query: 181 LDKLAYTLPMKGIRIGAVQLKISPAVFKPDPTGSGQTMIDSGSELTYLVDEAYNNVRAEI 240
           LDKLAYTLPMKGIRIGAVQLKISPAVFKPDPTGSGQTMIDSGSELTYLVDEAYNNVRAEI
Sbjct: 181 LDKLAYTLPMKGIRIGAVQLKISPAVFKPDPTGSGQTMIDSGSELTYLVDEAYNNVRAEI 240

Query: 241 VRLVGPMMKKGYEYASVADMCFDGAMAAAAGRRIGEMWFQFENGVEILVGKGEGLLTVVE 300
           VRLVGPMMKKGYEYASVADMCFDGAMAAAAGRRIGEMWFQFENGVEILVGKGEGLLTVVE
Sbjct: 241 VRLVGPMMKKGYEYASVADMCFDGAMAAAAGRRIGEMWFQFENGVEILVGKGEGLLTVVE 300

Query: 301 KGVKCVGIGRSGRLGTESNMIGNVHQQNMWVEYDLANKRVGFGGAVCSGLKA 353
           KGVKCVGIGRSGRLGTESNMIGNVHQQNMWVEYDLANKRVGFGGAVCSGLKA
Sbjct: 301 KGVKCVGIGRSGRLGTESNMIGNVHQQNMWVEYDLANKRVGFGGAVCSGLKA 352

BLAST of CmoCh01G003500 vs. ExPASy TrEMBL
Match: A0A6J1K7E8 (aspartic proteinase PCS1-like OS=Cucurbita maxima OX=3661 GN=LOC111492941 PE=3 SV=1)

HSP 1 Score: 710.7 bits (1833), Expect = 3.1e-201
Identity = 341/352 (96.88%), Postives = 345/352 (98.01%), Query Frame = 0

Query: 1   MGTPPQLMDMVLDTGSQLSWIQCHGTVNGKSVKPRFKWFNPSLSSTFSFLPCNSSLCKPR 60
           MGTPPQLMDMVLDTGSQLSWIQCHG VNGKSV+PR KWFNPSLSSTFSFLPCNSSLCKPR
Sbjct: 71  MGTPPQLMDMVLDTGSQLSWIQCHGKVNGKSVQPRIKWFNPSLSSTFSFLPCNSSLCKPR 130

Query: 61  IPDFTLPTSCDPRRHCHYSYFYADGTLAEGNLVTEKFSFTASPIAIGCVKPSAENRGILG 120
           IPDFTLPTSCDPRRHCHYSYFYADGTLAEGNLVTEKFSFTASP+AIGCVKPSAENRGILG
Sbjct: 131 IPDFTLPTSCDPRRHCHYSYFYADGTLAEGNLVTEKFSFTASPVAIGCVKPSAENRGILG 190

Query: 121 MNTGHLSFISQAKISKFSYCVPGRSRSDLTGLFYLGDNPNSGKFKYVNMLTFPESQNSPN 180
           MNTGHLSFISQAKISKFSYCVPGRSRSDLTGLFYLGDNPNSGKFKYVNMLTFPESQNSPN
Sbjct: 191 MNTGHLSFISQAKISKFSYCVPGRSRSDLTGLFYLGDNPNSGKFKYVNMLTFPESQNSPN 250

Query: 181 LDKLAYTLPMKGIRIGAVQLKISPAVFKPDPTGSGQTMIDSGSELTYLVDEAYNNVRAEI 240
           LDKLAYTLPMKGIRIG V LKISPAVFKPDPTGSGQTMIDSGSELTYLVDEAYN VRAEI
Sbjct: 251 LDKLAYTLPMKGIRIGRVHLKISPAVFKPDPTGSGQTMIDSGSELTYLVDEAYNKVRAEI 310

Query: 241 VRLVGPMMKKGYEYASVADMCFDGAMAAAAGRRIGEMWFQFENGVEILVGKGEGLLTVVE 300
           VRLVGPMMKKGYEYASV+DMCFD AMAAAAGRRIG+MWFQFENGVEILVGKGEGLLT VE
Sbjct: 311 VRLVGPMMKKGYEYASVSDMCFDAAMAAAAGRRIGDMWFQFENGVEILVGKGEGLLTEVE 370

Query: 301 KGVKCVGIGRSGRLGTESNMIGNVHQQNMWVEYDLANKRVGFGGAVCSGLKA 353
           KGVKCVGIGRSGRLGTESNMIGNVHQQNMWVEYDLANKRVGFGGAVCSGLKA
Sbjct: 371 KGVKCVGIGRSGRLGTESNMIGNVHQQNMWVEYDLANKRVGFGGAVCSGLKA 422

BLAST of CmoCh01G003500 vs. ExPASy TrEMBL
Match: A0A6J1IBE7 (aspartic proteinase PCS1-like OS=Cucurbita maxima OX=3661 GN=LOC111471393 PE=3 SV=1)

HSP 1 Score: 613.2 bits (1580), Expect = 6.7e-172
Identity = 292/356 (82.02%), Postives = 320/356 (89.89%), Query Frame = 0

Query: 1   MGTPPQLMDMVLDTGSQLSWIQCHGTVNGKSVKPRFKWFNPSLSSTFSFLPCNSSLCKPR 60
           +G+PPQ MDMV+DTGSQLSWIQCHG V  KSVKP   WF+P LSS+FSFLPCN++LC+PR
Sbjct: 65  IGSPPQQMDMVVDTGSQLSWIQCHGKVRRKSVKPMINWFDPYLSSSFSFLPCNTTLCRPR 124

Query: 61  IPDFTLPTSCDPRRHCHYSYFYADGTLAEGNLVTEKF----SFTASPIAIGCVKPSAENR 120
           IPDFTLPTSCDP RHCHYSYFYADGTLAEGNLVTEKF    SFT   + +GC   S +NR
Sbjct: 125 IPDFTLPTSCDPTRHCHYSYFYADGTLAEGNLVTEKFTFSSSFTTRSLLLGCATASTQNR 184

Query: 121 GILGMNTGHLSFISQAKISKFSYCVPGRSRSDLTGLFYLGDNPNSGKFKYVNMLTFPESQ 180
           G+LGMNTG LSFISQAKISKFSYCVP R+ SDLTGLFYLGDNPNS KFKYVNMLTFP+S+
Sbjct: 185 GMLGMNTGRLSFISQAKISKFSYCVPDRTGSDLTGLFYLGDNPNSAKFKYVNMLTFPKSR 244

Query: 181 NSPNLDKLAYTLPMKGIRIGAVQLKISPAVFKPDPTGSGQTMIDSGSELTYLVDEAYNNV 240
            SPNLDKLAYTLPMKGIRIG  +L ISPAVFKPDP+G+GQTMIDSGS+LTYLVDEAY+ V
Sbjct: 245 RSPNLDKLAYTLPMKGIRIGNNKLNISPAVFKPDPSGAGQTMIDSGSDLTYLVDEAYSKV 304

Query: 241 RAEIVRLVGPMMKKGYEYASVADMCFDGAMAAAAGRRIGEMWFQFENGVEILVGKGEGLL 300
           RAE+VRLVGPMMKKGYEYA+VADMCFDGA+AA  GRRIG+MWFQFENGVEILVGKGEGLL
Sbjct: 305 RAEMVRLVGPMMKKGYEYAAVADMCFDGAVAAVVGRRIGDMWFQFENGVEILVGKGEGLL 364

Query: 301 TVVEKGVKCVGIGRSGRLGTESNMIGNVHQQNMWVEYDLANKRVGFGGAVCSGLKA 353
           T VEKGVKCVGIGRS RL TESN+IGNVHQQNMWVEYDL+NKR+GFG A+CSGLKA
Sbjct: 365 TEVEKGVKCVGIGRSDRLVTESNIIGNVHQQNMWVEYDLSNKRIGFGVAMCSGLKA 420

BLAST of CmoCh01G003500 vs. ExPASy TrEMBL
Match: A0A6J1I612 (aspartic proteinase PCS1-like OS=Cucurbita maxima OX=3661 GN=LOC111471392 PE=3 SV=1)

HSP 1 Score: 612.8 bits (1579), Expect = 8.7e-172
Identity = 291/356 (81.74%), Postives = 322/356 (90.45%), Query Frame = 0

Query: 1   MGTPPQLMDMVLDTGSQLSWIQCHGTVNGKSVKPRFKWFNPSLSSTFSFLPCNSSLCKPR 60
           +G+PPQ MDMV+DTGSQLSWIQCHG V  KSVKP   WF+P LSS+FSFLPCN++LC+PR
Sbjct: 65  IGSPPQQMDMVVDTGSQLSWIQCHGKVRRKSVKPMINWFDPYLSSSFSFLPCNTTLCRPR 124

Query: 61  IPDFTLPTSCDPRRHCHYSYFYADGTLAEGNLVTEKFSFTAS----PIAIGCVKPSAENR 120
           IPDFTLPTSCDP RHCHYSYFYADGTLAEGNLVTEKF+F++S     + +GC   S +NR
Sbjct: 125 IPDFTLPTSCDPTRHCHYSYFYADGTLAEGNLVTEKFTFSSSLTTRSLLLGCATASTQNR 184

Query: 121 GILGMNTGHLSFISQAKISKFSYCVPGRSRSDLTGLFYLGDNPNSGKFKYVNMLTFPESQ 180
           G+LGMNTG LSFISQAKISKFSYCVP R+ SDLTGLFYLGDNPNS KFKYVNMLTFP+S+
Sbjct: 185 GMLGMNTGRLSFISQAKISKFSYCVPDRTGSDLTGLFYLGDNPNSAKFKYVNMLTFPKSR 244

Query: 181 NSPNLDKLAYTLPMKGIRIGAVQLKISPAVFKPDPTGSGQTMIDSGSELTYLVDEAYNNV 240
            SPNLDKLAYTLPMKGIRIG  +L ISPAVFKPDP+G+GQTMIDSGS+LTYLVDEAY+ V
Sbjct: 245 RSPNLDKLAYTLPMKGIRIGNNKLNISPAVFKPDPSGAGQTMIDSGSDLTYLVDEAYSKV 304

Query: 241 RAEIVRLVGPMMKKGYEYASVADMCFDGAMAAAAGRRIGEMWFQFENGVEILVGKGEGLL 300
           RAE+VRLVGPMMKKGYEYA+VADMCFDGA+AA  GRRIG+MWFQFENGVEILVGKGEGLL
Sbjct: 305 RAEMVRLVGPMMKKGYEYAAVADMCFDGAVAAVVGRRIGDMWFQFENGVEILVGKGEGLL 364

Query: 301 TVVEKGVKCVGIGRSGRLGTESNMIGNVHQQNMWVEYDLANKRVGFGGAVCSGLKA 353
           T VEKGVKCVGIGRS RL TESN+IGNVHQQNMWVEYDL+NKR+GFG A+CSGLKA
Sbjct: 365 TEVEKGVKCVGIGRSDRLVTESNIIGNVHQQNMWVEYDLSNKRIGFGVAMCSGLKA 420

BLAST of CmoCh01G003500 vs. ExPASy TrEMBL
Match: A0A6J1ICY2 (aspartic proteinase PCS1-like OS=Cucurbita maxima OX=3661 GN=LOC111471416 PE=3 SV=1)

HSP 1 Score: 611.7 bits (1576), Expect = 1.9e-171
Identity = 291/356 (81.74%), Postives = 321/356 (90.17%), Query Frame = 0

Query: 1   MGTPPQLMDMVLDTGSQLSWIQCHGTVNGKSVKPRFKWFNPSLSSTFSFLPCNSSLCKPR 60
           +G+PPQ MDMV+DTGSQLSWIQCHG V  KSVKP   WF+P LSS+FSFLPCN++LC+PR
Sbjct: 61  IGSPPQQMDMVVDTGSQLSWIQCHGKVRRKSVKPMINWFDPYLSSSFSFLPCNTTLCRPR 120

Query: 61  IPDFTLPTSCDPRRHCHYSYFYADGTLAEGNLVTEKFSFTAS----PIAIGCVKPSAENR 120
           IPDFTLPTSCDP RHCHYSYFYADGTLAEGNLVTEKF+F++S     + +GC   S +NR
Sbjct: 121 IPDFTLPTSCDPTRHCHYSYFYADGTLAEGNLVTEKFTFSSSLTTRSLLLGCATASTQNR 180

Query: 121 GILGMNTGHLSFISQAKISKFSYCVPGRSRSDLTGLFYLGDNPNSGKFKYVNMLTFPESQ 180
           G+LGMNTG LSFISQAKISKFSYCVP R+ SDLTGLFYLGDNPNS KFKYVNMLTFP+S+
Sbjct: 181 GMLGMNTGRLSFISQAKISKFSYCVPDRTGSDLTGLFYLGDNPNSAKFKYVNMLTFPKSR 240

Query: 181 NSPNLDKLAYTLPMKGIRIGAVQLKISPAVFKPDPTGSGQTMIDSGSELTYLVDEAYNNV 240
            SPNLDKLAYTLPMKGIRIG  +L ISPAVFKPDP+G+GQTMIDSGS+LTYLVDEAY+ V
Sbjct: 241 RSPNLDKLAYTLPMKGIRIGNNKLNISPAVFKPDPSGAGQTMIDSGSDLTYLVDEAYSKV 300

Query: 241 RAEIVRLVGPMMKKGYEYASVADMCFDGAMAAAAGRRIGEMWFQFENGVEILVGKGEGLL 300
           RAE+VRLVGPMMKKGYEYA+VADMCFDGA+AA  GRRIG+MWFQFENGVEILVGKGEGLL
Sbjct: 301 RAEMVRLVGPMMKKGYEYAAVADMCFDGAVAAVVGRRIGDMWFQFENGVEILVGKGEGLL 360

Query: 301 TVVEKGVKCVGIGRSGRLGTESNMIGNVHQQNMWVEYDLANKRVGFGGAVCSGLKA 353
           T VEKGVKCVGIGRS RL TESN+IGNVHQQNMWVEYDL+NKR+GFG A CSGLKA
Sbjct: 361 TEVEKGVKCVGIGRSDRLVTESNIIGNVHQQNMWVEYDLSNKRIGFGVAKCSGLKA 416

BLAST of CmoCh01G003500 vs. NCBI nr
Match: XP_022949043.1 (aspartic proteinase PCS1-like [Cucurbita moschata])

HSP 1 Score: 730.3 bits (1884), Expect = 7.7e-207
Identity = 352/352 (100.00%), Postives = 352/352 (100.00%), Query Frame = 0

Query: 1   MGTPPQLMDMVLDTGSQLSWIQCHGTVNGKSVKPRFKWFNPSLSSTFSFLPCNSSLCKPR 60
           MGTPPQLMDMVLDTGSQLSWIQCHGTVNGKSVKPRFKWFNPSLSSTFSFLPCNSSLCKPR
Sbjct: 1   MGTPPQLMDMVLDTGSQLSWIQCHGTVNGKSVKPRFKWFNPSLSSTFSFLPCNSSLCKPR 60

Query: 61  IPDFTLPTSCDPRRHCHYSYFYADGTLAEGNLVTEKFSFTASPIAIGCVKPSAENRGILG 120
           IPDFTLPTSCDPRRHCHYSYFYADGTLAEGNLVTEKFSFTASPIAIGCVKPSAENRGILG
Sbjct: 61  IPDFTLPTSCDPRRHCHYSYFYADGTLAEGNLVTEKFSFTASPIAIGCVKPSAENRGILG 120

Query: 121 MNTGHLSFISQAKISKFSYCVPGRSRSDLTGLFYLGDNPNSGKFKYVNMLTFPESQNSPN 180
           MNTGHLSFISQAKISKFSYCVPGRSRSDLTGLFYLGDNPNSGKFKYVNMLTFPESQNSPN
Sbjct: 121 MNTGHLSFISQAKISKFSYCVPGRSRSDLTGLFYLGDNPNSGKFKYVNMLTFPESQNSPN 180

Query: 181 LDKLAYTLPMKGIRIGAVQLKISPAVFKPDPTGSGQTMIDSGSELTYLVDEAYNNVRAEI 240
           LDKLAYTLPMKGIRIGAVQLKISPAVFKPDPTGSGQTMIDSGSELTYLVDEAYNNVRAEI
Sbjct: 181 LDKLAYTLPMKGIRIGAVQLKISPAVFKPDPTGSGQTMIDSGSELTYLVDEAYNNVRAEI 240

Query: 241 VRLVGPMMKKGYEYASVADMCFDGAMAAAAGRRIGEMWFQFENGVEILVGKGEGLLTVVE 300
           VRLVGPMMKKGYEYASVADMCFDGAMAAAAGRRIGEMWFQFENGVEILVGKGEGLLTVVE
Sbjct: 241 VRLVGPMMKKGYEYASVADMCFDGAMAAAAGRRIGEMWFQFENGVEILVGKGEGLLTVVE 300

Query: 301 KGVKCVGIGRSGRLGTESNMIGNVHQQNMWVEYDLANKRVGFGGAVCSGLKA 353
           KGVKCVGIGRSGRLGTESNMIGNVHQQNMWVEYDLANKRVGFGGAVCSGLKA
Sbjct: 301 KGVKCVGIGRSGRLGTESNMIGNVHQQNMWVEYDLANKRVGFGGAVCSGLKA 352

BLAST of CmoCh01G003500 vs. NCBI nr
Match: KAG6606984.1 (Aspartic proteinase PCS1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 716.1 bits (1847), Expect = 1.5e-202
Identity = 345/352 (98.01%), Postives = 348/352 (98.86%), Query Frame = 0

Query: 1   MGTPPQLMDMVLDTGSQLSWIQCHGTVNGKSVKPRFKWFNPSLSSTFSFLPCNSSLCKPR 60
           MGTPPQLMDMVLDTGSQLSWIQCHG V+GKSVKP FKWFNPSLSSTFSFLPCNSSLCKPR
Sbjct: 71  MGTPPQLMDMVLDTGSQLSWIQCHGKVDGKSVKPGFKWFNPSLSSTFSFLPCNSSLCKPR 130

Query: 61  IPDFTLPTSCDPRRHCHYSYFYADGTLAEGNLVTEKFSFTASPIAIGCVKPSAENRGILG 120
           IPDFTLPTSCDPRRHCHYSYFYADGTLAEGNLVTEKFSFTASPIAIGCVKPSAENRGILG
Sbjct: 131 IPDFTLPTSCDPRRHCHYSYFYADGTLAEGNLVTEKFSFTASPIAIGCVKPSAENRGILG 190

Query: 121 MNTGHLSFISQAKISKFSYCVPGRSRSDLTGLFYLGDNPNSGKFKYVNMLTFPESQNSPN 180
           MNTGHLSFISQAKISKFSYCVPGRSRSDLTGLFYLGDNPNSGKFKYVNMLTFPESQNSP+
Sbjct: 191 MNTGHLSFISQAKISKFSYCVPGRSRSDLTGLFYLGDNPNSGKFKYVNMLTFPESQNSPS 250

Query: 181 LDKLAYTLPMKGIRIGAVQLKISPAVFKPDPTGSGQTMIDSGSELTYLVDEAYNNVRAEI 240
           LDKLAYTLPMKGIRIG VQLKISPAVFKPDPTG+GQTMIDSGSELTYLVDEAYN VRAEI
Sbjct: 251 LDKLAYTLPMKGIRIGRVQLKISPAVFKPDPTGAGQTMIDSGSELTYLVDEAYNKVRAEI 310

Query: 241 VRLVGPMMKKGYEYASVADMCFDGAMAAAAGRRIGEMWFQFENGVEILVGKGEGLLTVVE 300
           VRLVGPMMKKGYEYASVADMCFDGAMAAAAGRRIGEMWFQFENGVEILVGKGEGLLTVVE
Sbjct: 311 VRLVGPMMKKGYEYASVADMCFDGAMAAAAGRRIGEMWFQFENGVEILVGKGEGLLTVVE 370

Query: 301 KGVKCVGIGRSGRLGTESNMIGNVHQQNMWVEYDLANKRVGFGGAVCSGLKA 353
           KGVKCVGIGRSGRLGTESNMIGNVHQQNMWVEYDLANKRVGFGGAVCSGLKA
Sbjct: 371 KGVKCVGIGRSGRLGTESNMIGNVHQQNMWVEYDLANKRVGFGGAVCSGLKA 422

BLAST of CmoCh01G003500 vs. NCBI nr
Match: XP_022998247.1 (aspartic proteinase PCS1-like [Cucurbita maxima])

HSP 1 Score: 710.7 bits (1833), Expect = 6.3e-201
Identity = 341/352 (96.88%), Postives = 345/352 (98.01%), Query Frame = 0

Query: 1   MGTPPQLMDMVLDTGSQLSWIQCHGTVNGKSVKPRFKWFNPSLSSTFSFLPCNSSLCKPR 60
           MGTPPQLMDMVLDTGSQLSWIQCHG VNGKSV+PR KWFNPSLSSTFSFLPCNSSLCKPR
Sbjct: 71  MGTPPQLMDMVLDTGSQLSWIQCHGKVNGKSVQPRIKWFNPSLSSTFSFLPCNSSLCKPR 130

Query: 61  IPDFTLPTSCDPRRHCHYSYFYADGTLAEGNLVTEKFSFTASPIAIGCVKPSAENRGILG 120
           IPDFTLPTSCDPRRHCHYSYFYADGTLAEGNLVTEKFSFTASP+AIGCVKPSAENRGILG
Sbjct: 131 IPDFTLPTSCDPRRHCHYSYFYADGTLAEGNLVTEKFSFTASPVAIGCVKPSAENRGILG 190

Query: 121 MNTGHLSFISQAKISKFSYCVPGRSRSDLTGLFYLGDNPNSGKFKYVNMLTFPESQNSPN 180
           MNTGHLSFISQAKISKFSYCVPGRSRSDLTGLFYLGDNPNSGKFKYVNMLTFPESQNSPN
Sbjct: 191 MNTGHLSFISQAKISKFSYCVPGRSRSDLTGLFYLGDNPNSGKFKYVNMLTFPESQNSPN 250

Query: 181 LDKLAYTLPMKGIRIGAVQLKISPAVFKPDPTGSGQTMIDSGSELTYLVDEAYNNVRAEI 240
           LDKLAYTLPMKGIRIG V LKISPAVFKPDPTGSGQTMIDSGSELTYLVDEAYN VRAEI
Sbjct: 251 LDKLAYTLPMKGIRIGRVHLKISPAVFKPDPTGSGQTMIDSGSELTYLVDEAYNKVRAEI 310

Query: 241 VRLVGPMMKKGYEYASVADMCFDGAMAAAAGRRIGEMWFQFENGVEILVGKGEGLLTVVE 300
           VRLVGPMMKKGYEYASV+DMCFD AMAAAAGRRIG+MWFQFENGVEILVGKGEGLLT VE
Sbjct: 311 VRLVGPMMKKGYEYASVSDMCFDAAMAAAAGRRIGDMWFQFENGVEILVGKGEGLLTEVE 370

Query: 301 KGVKCVGIGRSGRLGTESNMIGNVHQQNMWVEYDLANKRVGFGGAVCSGLKA 353
           KGVKCVGIGRSGRLGTESNMIGNVHQQNMWVEYDLANKRVGFGGAVCSGLKA
Sbjct: 371 KGVKCVGIGRSGRLGTESNMIGNVHQQNMWVEYDLANKRVGFGGAVCSGLKA 422

BLAST of CmoCh01G003500 vs. NCBI nr
Match: XP_023525773.1 (aspartic proteinase PCS1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 710.3 bits (1832), Expect = 8.3e-201
Identity = 341/352 (96.88%), Postives = 345/352 (98.01%), Query Frame = 0

Query: 1   MGTPPQLMDMVLDTGSQLSWIQCHGTVNGKSVKPRFKWFNPSLSSTFSFLPCNSSLCKPR 60
           MGTPPQLMDMVLDTGSQLSWIQCHG VNGKSVKP  KWFNPSLSSTFSFLPCNSSLCKPR
Sbjct: 71  MGTPPQLMDMVLDTGSQLSWIQCHGKVNGKSVKPGIKWFNPSLSSTFSFLPCNSSLCKPR 130

Query: 61  IPDFTLPTSCDPRRHCHYSYFYADGTLAEGNLVTEKFSFTASPIAIGCVKPSAENRGILG 120
           IPDFTLPTSCDPRRHCHYSYFYADGTLAEGNLVTEKFSFTASPIAIGCVKPSAENRGILG
Sbjct: 131 IPDFTLPTSCDPRRHCHYSYFYADGTLAEGNLVTEKFSFTASPIAIGCVKPSAENRGILG 190

Query: 121 MNTGHLSFISQAKISKFSYCVPGRSRSDLTGLFYLGDNPNSGKFKYVNMLTFPESQNSPN 180
           MNTGHLSFISQAKISKFSYC+PGRSRSDLTGLFYLGDNPNSGKFKYVNMLTFPESQNSPN
Sbjct: 191 MNTGHLSFISQAKISKFSYCIPGRSRSDLTGLFYLGDNPNSGKFKYVNMLTFPESQNSPN 250

Query: 181 LDKLAYTLPMKGIRIGAVQLKISPAVFKPDPTGSGQTMIDSGSELTYLVDEAYNNVRAEI 240
           LDKLAYTLPMKGIRIG VQLKISPAVFKPDPTGSGQTMIDSGSELTYLVDEAYN V+AEI
Sbjct: 251 LDKLAYTLPMKGIRIGRVQLKISPAVFKPDPTGSGQTMIDSGSELTYLVDEAYNKVKAEI 310

Query: 241 VRLVGPMMKKGYEYASVADMCFDGAMAAAAGRRIGEMWFQFENGVEILVGKGEGLLTVVE 300
           VRLVGPMMKKGYEYASVADMCFD AMAAAAG RIG+MWFQFENGVEILVGKGEGLLTVVE
Sbjct: 311 VRLVGPMMKKGYEYASVADMCFDSAMAAAAGSRIGDMWFQFENGVEILVGKGEGLLTVVE 370

Query: 301 KGVKCVGIGRSGRLGTESNMIGNVHQQNMWVEYDLANKRVGFGGAVCSGLKA 353
           KGVKCVGIGRSGRLGTESNMIGNVHQQNMWVEYDLANKRVGFGGA+CSGLKA
Sbjct: 371 KGVKCVGIGRSGRLGTESNMIGNVHQQNMWVEYDLANKRVGFGGALCSGLKA 422

BLAST of CmoCh01G003500 vs. NCBI nr
Match: XP_022972883.1 (aspartic proteinase PCS1-like [Cucurbita maxima])

HSP 1 Score: 613.2 bits (1580), Expect = 1.4e-171
Identity = 292/356 (82.02%), Postives = 320/356 (89.89%), Query Frame = 0

Query: 1   MGTPPQLMDMVLDTGSQLSWIQCHGTVNGKSVKPRFKWFNPSLSSTFSFLPCNSSLCKPR 60
           +G+PPQ MDMV+DTGSQLSWIQCHG V  KSVKP   WF+P LSS+FSFLPCN++LC+PR
Sbjct: 65  IGSPPQQMDMVVDTGSQLSWIQCHGKVRRKSVKPMINWFDPYLSSSFSFLPCNTTLCRPR 124

Query: 61  IPDFTLPTSCDPRRHCHYSYFYADGTLAEGNLVTEKF----SFTASPIAIGCVKPSAENR 120
           IPDFTLPTSCDP RHCHYSYFYADGTLAEGNLVTEKF    SFT   + +GC   S +NR
Sbjct: 125 IPDFTLPTSCDPTRHCHYSYFYADGTLAEGNLVTEKFTFSSSFTTRSLLLGCATASTQNR 184

Query: 121 GILGMNTGHLSFISQAKISKFSYCVPGRSRSDLTGLFYLGDNPNSGKFKYVNMLTFPESQ 180
           G+LGMNTG LSFISQAKISKFSYCVP R+ SDLTGLFYLGDNPNS KFKYVNMLTFP+S+
Sbjct: 185 GMLGMNTGRLSFISQAKISKFSYCVPDRTGSDLTGLFYLGDNPNSAKFKYVNMLTFPKSR 244

Query: 181 NSPNLDKLAYTLPMKGIRIGAVQLKISPAVFKPDPTGSGQTMIDSGSELTYLVDEAYNNV 240
            SPNLDKLAYTLPMKGIRIG  +L ISPAVFKPDP+G+GQTMIDSGS+LTYLVDEAY+ V
Sbjct: 245 RSPNLDKLAYTLPMKGIRIGNNKLNISPAVFKPDPSGAGQTMIDSGSDLTYLVDEAYSKV 304

Query: 241 RAEIVRLVGPMMKKGYEYASVADMCFDGAMAAAAGRRIGEMWFQFENGVEILVGKGEGLL 300
           RAE+VRLVGPMMKKGYEYA+VADMCFDGA+AA  GRRIG+MWFQFENGVEILVGKGEGLL
Sbjct: 305 RAEMVRLVGPMMKKGYEYAAVADMCFDGAVAAVVGRRIGDMWFQFENGVEILVGKGEGLL 364

Query: 301 TVVEKGVKCVGIGRSGRLGTESNMIGNVHQQNMWVEYDLANKRVGFGGAVCSGLKA 353
           T VEKGVKCVGIGRS RL TESN+IGNVHQQNMWVEYDL+NKR+GFG A+CSGLKA
Sbjct: 365 TEVEKGVKCVGIGRSDRLVTESNIIGNVHQQNMWVEYDLSNKRIGFGVAMCSGLKA 420

BLAST of CmoCh01G003500 vs. TAIR 10
Match: AT5G37540.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 453.8 bits (1166), Expect = 1.3e-127
Identity = 226/354 (63.84%), Postives = 264/354 (74.58%), Query Frame = 0

Query: 1   MGTPPQLMDMVLDTGSQLSWIQCHGTVNGKSVKPRFKWFNPSLSSTFSFLPCNSSLCKPR 60
           +GTP Q  ++VLDTGSQLSWIQCH     K + P    F+PSLSS+FS LPC+  LCKPR
Sbjct: 86  IGTPSQSQELVLDTGSQLSWIQCHPKKIKKPLPPPTTSFDPSLSSSFSDLPCSHPLCKPR 145

Query: 61  IPDFTLPTSCDPRRHCHYSYFYADGTLAEGNLVTEKFSF----TASPIAIGCVKPSAENR 120
           IPDFTLPTSCD  R CHYSYFYADGT AEGNLV EKF+F    T  P+ +GC K S + +
Sbjct: 146 IPDFTLPTSCDSNRLCHYSYFYADGTFAEGNLVKEKFTFSNSQTTPPLILGCAKESTDEK 205

Query: 121 GILGMNTGHLSFISQAKISKFSYCVPGRSRSD---LTGLFYLGDNPNSGKFKYVNMLTFP 180
           GILGMN G LSFISQAKISKFSYC+P RS       TG FYLGDNPNS  FKYV++LTFP
Sbjct: 206 GILGMNLGRLSFISQAKISKFSYCIPTRSNRPGLASTGSFYLGDNPNSRGFKYVSLLTFP 265

Query: 181 ESQNSPNLDKLAYTLPMKGIRIGAVQLKISPAVFKPDPTGSGQTMIDSGSELTYLVDEAY 240
           +SQ  PNLD LAYT+P++GIRIG  +L I  +VF+PD  GSGQTM+DSGSE T+LVD AY
Sbjct: 266 QSQRMPNLDPLAYTVPLQGIRIGQKRLNIPGSVFRPDAGGSGQTMVDSGSEFTHLVDVAY 325

Query: 241 NNVRAEIVRLVGPMMKKGYEYASVADMCFDGAMAAAAGRRIGEMWFQFENGVEILVGKGE 300
           + V+ EIVRLVG  +KKGY Y S ADMCFDG  +   GR IG++ F+F  GVEILV K +
Sbjct: 326 DKVKEEIVRLVGSRLKKGYVYGSTADMCFDGNHSMEIGRLIGDLVFEFGRGVEILVEK-Q 385

Query: 301 GLLTVVEKGVKCVGIGRSGRLGTESNMIGNVHQQNMWVEYDLANKRVGFGGAVC 348
            LL  V  G+ CVGIGRS  LG  SN+IGNVHQQN+WVE+D+ N+RVGF  A C
Sbjct: 386 SLLVNVGGGIHCVGIGRSSMLGAASNIIGNVHQQNLWVEFDVTNRRVGFSKAEC 438

BLAST of CmoCh01G003500 vs. TAIR 10
Match: AT1G66180.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 440.3 bits (1131), Expect = 1.5e-123
Identity = 226/356 (63.48%), Postives = 263/356 (73.88%), Query Frame = 0

Query: 1   MGTPPQLMDMVLDTGSQLSWIQCHGTVNGKSVKPRFKW-FNPSLSSTFSFLPCNSSLCKP 60
           +GTPPQ   MVLDTGSQLSWIQCH     K + P+ K  F+PSLSS+FS LPC+  LCKP
Sbjct: 78  IGTPPQAQQMVLDTGSQLSWIQCH----RKKLPPKPKTSFDPSLSSSFSTLPCSHPLCKP 137

Query: 61  RIPDFTLPTSCDPRRHCHYSYFYADGTLAEGNLVTEKFSFT----ASPIAIGCVKPSAEN 120
           RIPDFTLPTSCD  R CHYSYFYADGT AEGNLV EK +F+      P+ +GC   S+++
Sbjct: 138 RIPDFTLPTSCDSNRLCHYSYFYADGTFAEGNLVKEKITFSNTEITPPLILGCATESSDD 197

Query: 121 RGILGMNTGHLSFISQAKISKFSYCVPGRSRS---DLTGLFYLGDNPNSGKFKYVNMLTF 180
           RGILGMN G LSF+SQAKISKFSYC+P +S       TG FYLGDNPNS  FKYV++LTF
Sbjct: 198 RGILGMNRGRLSFVSQAKISKFSYCIPPKSNRPGFTPTGSFYLGDNPNSHGFKYVSLLTF 257

Query: 181 PESQNSPNLDKLAYTLPMKGIRIGAVQLKISPAVFKPDPTGSGQTMIDSGSELTYLVDEA 240
           PESQ  PNLD LAYT+PM GIR G  +L IS +VF+PD  GSGQTM+DSGSE T+LVD A
Sbjct: 258 PESQRMPNLDPLAYTVPMIGIRFGLKKLNISGSVFRPDAGGSGQTMVDSGSEFTHLVDAA 317

Query: 241 YNNVRAEIVRLVGPMMKKGYEYASVADMCFDGAMAAAAGRRIGEMWFQFENGVEILVGKG 300
           Y+ VRAEI+  VG  +KKGY Y   ADMCFDG +A    R IG++ F F  GVEILV K 
Sbjct: 318 YDKVRAEIMTRVGRRLKKGYVYGGTADMCFDGNVAMIP-RLIGDLVFVFTRGVEILVPK- 377

Query: 301 EGLLTVVEKGVKCVGIGRSGRLGTESNMIGNVHQQNMWVEYDLANKRVGFGGAVCS 349
           E +L  V  G+ CVGIGRS  LG  SN+IGNVHQQN+WVE+D+ N+RVGF  A CS
Sbjct: 378 ERVLVNVGGGIHCVGIGRSSMLGAASNIIGNVHQQNLWVEFDVTNRRVGFAKADCS 427

BLAST of CmoCh01G003500 vs. TAIR 10
Match: AT2G39710.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 234.6 bits (597), Expect = 1.2e-61
Identity = 141/371 (38.01%), Postives = 203/371 (54.72%), Query Frame = 0

Query: 1   MGTPPQLMDMVLDTGSQLSWIQCHGTVNGKSVKPRFKWFNPSLSSTFSFLPCNSSLCKPR 60
           +G PPQ + MVLDTGS+LSW+ C  + N  SV      FNP  SST+S +PC+S +C+ R
Sbjct: 71  VGDPPQNISMVLDTGSELSWLHCKKSPNLGSV------FNPVSSSTYSPVPCSSPICRTR 130

Query: 61  IPDFTLPTSCDPRRH-CHYSYFYADGTLAEGNLVTEKF---SFTASPIAIGCV------- 120
             D  +P SCDP+ H CH +  YAD T  EGNL  E F   S T      GC+       
Sbjct: 131 TRDLPIPASCDPKTHLCHVAISYADATSIEGNLAHETFVIGSVTRPGTLFGCMDSGLSSN 190

Query: 121 -KPSAENRGILGMNTGHLSFISQAKISKFSYCVPGRSRSDLTGLFYLGDNPNS--GKFKY 180
            +  A++ G++GMN G LSF++Q   SKFSYC+ G   SD +G   LGD   S  G  +Y
Sbjct: 191 SEEDAKSTGLMGMNRGSLSFVNQLGFSKFSYCISG---SDSSGFLLLGDASYSWLGPIQY 250

Query: 181 VNMLTFPESQNSPNLDKLAYTLPMKGIRIGAVQLKISPAVFKPDPTGSGQTMIDSGSELT 240
             ++   +S   P  D++AYT+ ++GIR+G+  L +  +VF PD TG+GQTM+DSG++ T
Sbjct: 251 TPLVL--QSTPLPYFDRVAYTVQLEGIRVGSKILSLPKSVFVPDHTGAGQTMVDSGTQFT 310

Query: 241 YLVDEAYNNVRAEIVRLVGPMMK----KGYEYASVADMCFDGAMAAAAGRRIGEMWFQFE 300
           +L+   Y  ++ E +     +++      + +    D+C+              M     
Sbjct: 311 FLMGPVYTALKNEFITQTKSVLRLVDDPDFVFQGTMDLCYKVGSTTRPNFSGLPMVSLMF 370

Query: 301 NGVEILVGKGEGLLTVV-------EKGVKCVGIGRSGRLGTESNMIGNVHQQNMWVEYDL 347
            G E+ V  G+ LL  V       ++ V C   G S  LG E+ +IG+ HQQN+W+E+DL
Sbjct: 371 RGAEMSV-SGQKLLYRVNGAGSEGKEEVYCFTFGNSDLLGIEAFVIGHHHQQNVWMEFDL 429

BLAST of CmoCh01G003500 vs. TAIR 10
Match: AT5G02190.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 221.9 bits (564), Expect = 8.3e-58
Identity = 133/376 (35.37%), Postives = 197/376 (52.39%), Query Frame = 0

Query: 1   MGTPPQLMDMVLDTGSQLSWIQCHGTVNGKSVKPRFKWFNPSLSSTFSFLPCNSSLCKPR 60
           +GTPPQ + MV+DTGS+LSW++C+ + N   V      F+P+ SS++S +PC+S  C+ R
Sbjct: 79  VGTPPQNISMVIDTGSELSWLRCNRSSNPNPVNN----FDPTRSSSYSPIPCSSPTCRTR 138

Query: 61  IPDFTLPTSCDPRRHCHYSYFYADGTLAEGNLVTEKFSF----TASPIAIGCV------- 120
             DF +P SCD  + CH +  YAD + +EGNL  E F F      S +  GC+       
Sbjct: 139 TRDFLIPASCDSDKLCHATLSYADASSSEGNLAAEIFHFGNSTNDSNLIFGCMGSVSGSD 198

Query: 121 -KPSAENRGILGMNTGHLSFISQAKISKFSYCVPGRSRSDLTGLFYLGDNPNSGKFKYVN 180
            +   +  G+LGMN G LSFISQ    KFSYC+ G    D  G   LGD+     F ++ 
Sbjct: 199 PEEDTKTTGLLGMNRGSLSFISQMGFPKFSYCISG--TDDFPGFLLLGDS----NFTWLT 258

Query: 181 MLTFPE----SQNSPNLDKLAYTLPMKGIRIGAVQLKISPAVFKPDPTGSGQTMIDSGSE 240
            L +      S   P  D++AYT+ + GI++    L I  +V  PD TG+GQTM+DSG++
Sbjct: 259 PLNYTPLIRISTPLPYFDRVAYTVQLTGIKVNGKLLPIPKSVLVPDHTGAGQTMVDSGTQ 318

Query: 241 LTYLVDEAYNNVRAEIVRLVGPMM----KKGYEYASVADMCF---DGAMAAAAGRRIGEM 300
            T+L+   Y  +R+  +     ++       + +    D+C+      + +    R+  +
Sbjct: 319 FTFLLGPVYTALRSHFLNRTNGILTVYEDPDFVFQGTMDLCYRISPVRIRSGILHRLPTV 378

Query: 301 WFQFENGVEILVGKGEGL------LTVVEKGVKCVGIGRSGRLGTESNMIGNVHQQNMWV 348
              FE G EI V  G+ L      LTV    V C   G S  +G E+ +IG+ HQQNMW+
Sbjct: 379 SLVFE-GAEIAV-SGQPLLYRVPHLTVGNDSVYCFTFGNSDLMGMEAYVIGHHHQQNMWI 438

BLAST of CmoCh01G003500 vs. TAIR 10
Match: AT3G18490.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 142.5 bits (358), Expect = 6.4e-34
Identity = 102/355 (28.73%), Postives = 165/355 (46.48%), Query Frame = 0

Query: 1   MGTPPQLMDMVLDTGSQLSWIQCHGTVNGKSVKPRFKWFNPSLSSTFSFLPCNSSLCKPR 60
           +GTP + M +VLDTGS ++WIQC    +    +     FNP+ SST+  L C++  C   
Sbjct: 168 VGTPAKEMYLVLDTGSDVNWIQCEPCAD--CYQQSDPVFNPTSSSTYKSLTCSAPQCS-- 227

Query: 61  IPDFTLPTSCDPRRHCHYSYFYADGTLAEGNLVTEKFSFTAS----PIAIGCVKPS---- 120
                L TS      C Y   Y DG+   G L T+  +F  S     +A+GC   +    
Sbjct: 228 ----LLETSACRSNKCLYQVSYGDGSFTVGELATDTVTFGNSGKINNVALGCGHDNEGLF 287

Query: 121 AENRGILGMNTGHLSFISQAKISKFSYCVPGRSRSDLTGLFYLGDNPNSGKFKYVNMLTF 180
               G+LG+  G LS  +Q K + FSYC+  R     + L +       G        T 
Sbjct: 288 TGAAGLLGLGGGVLSITNQMKATSFSYCLVDRDSGKSSSLDFNSVQLGGGD------ATA 347

Query: 181 PESQNSPNLDKLAYTLPMKGIRIGAVQLKISPAVFKPDPTGSGQTMIDSGSELTYLVDEA 240
           P  +N   +D   Y + + G  +G  ++ +  A+F  D +GSG  ++D G+ +T L  +A
Sbjct: 348 PLLRNK-KIDTF-YYVGLSGFSVGGEKVVLPDAIFDVDASGSGGVILDCGTAVTRLQTQA 407

Query: 241 YNNVRAEIVRLVGPMMKKGYEYASVADMCFDGAMAAAAGRRIGEMWFQFENGVEILVGKG 300
           YN++R   ++L    +KKG    S+ D C+D   ++ +  ++  + F F  G  + +   
Sbjct: 408 YNSLRDAFLKLT-VNLKKGSSSISLFDTCYD--FSSLSTVKVPTVAFHFTGGKSLDLPAK 467

Query: 301 EGLLTVVEKGVKCVGIGRSGRLGTESNMIGNVHQQNMWVEYDLANKRVGFGGAVC 348
             L+ V + G  C     +    +  ++IGNV QQ   + YDL+   +G  G  C
Sbjct: 468 NYLIPVDDSGTFCFAFAPT---SSSLSIIGNVQQQGTRITYDLSKNVIGLSGNKC 500

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9LZL31.2e-5635.37Aspartic proteinase PCS1 OS=Arabidopsis thaliana OX=3702 GN=PCS1 PE=2 SV=1[more]
Q766C31.5e-3531.46Aspartic proteinase nepenthesin-1 OS=Nepenthes gracilis OX=150966 GN=nep1 PE=1 S... [more]
Q766C22.5e-3532.59Aspartic proteinase nepenthesin-2 OS=Nepenthes gracilis OX=150966 GN=nep2 PE=1 S... [more]
Q9LS409.0e-3328.73Protein ASPARTIC PROTEASE IN GUARD CELL 1 OS=Arabidopsis thaliana OX=3702 GN=ASP... [more]
Q9LNJ32.5e-3030.28Aspartyl protease family protein 2 OS=Arabidopsis thaliana OX=3702 GN=APF2 PE=2 ... [more]
Match NameE-valueIdentityDescription
A0A6J1GAZ23.7e-207100.00aspartic proteinase PCS1-like OS=Cucurbita moschata OX=3662 GN=LOC111452506 PE=3... [more]
A0A6J1K7E83.1e-20196.88aspartic proteinase PCS1-like OS=Cucurbita maxima OX=3661 GN=LOC111492941 PE=3 S... [more]
A0A6J1IBE76.7e-17282.02aspartic proteinase PCS1-like OS=Cucurbita maxima OX=3661 GN=LOC111471393 PE=3 S... [more]
A0A6J1I6128.7e-17281.74aspartic proteinase PCS1-like OS=Cucurbita maxima OX=3661 GN=LOC111471392 PE=3 S... [more]
A0A6J1ICY21.9e-17181.74aspartic proteinase PCS1-like OS=Cucurbita maxima OX=3661 GN=LOC111471416 PE=3 S... [more]
Match NameE-valueIdentityDescription
XP_022949043.17.7e-207100.00aspartic proteinase PCS1-like [Cucurbita moschata][more]
KAG6606984.11.5e-20298.01Aspartic proteinase PCS1, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022998247.16.3e-20196.88aspartic proteinase PCS1-like [Cucurbita maxima][more]
XP_023525773.18.3e-20196.88aspartic proteinase PCS1-like [Cucurbita pepo subsp. pepo][more]
XP_022972883.11.4e-17182.02aspartic proteinase PCS1-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
AT5G37540.11.3e-12763.84Eukaryotic aspartyl protease family protein [more]
AT1G66180.11.5e-12363.48Eukaryotic aspartyl protease family protein [more]
AT2G39710.11.2e-6138.01Eukaryotic aspartyl protease family protein [more]
AT5G02190.18.3e-5835.37Eukaryotic aspartyl protease family protein [more]
AT3G18490.16.4e-3428.73Eukaryotic aspartyl protease family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001461Aspartic peptidase A1 familyPRINTSPR00792PEPSINcoord: 1..21
score: 43.46
coord: 319..334
score: 20.1
coord: 217..228
score: 28.66
IPR001461Aspartic peptidase A1 familyPANTHERPTHR47965ASPARTYL PROTEASE-RELATEDcoord: 2..348
IPR032861Xylanase inhibitor, N-terminalPFAMPF14543TAXi_Ncoord: 1..157
e-value: 1.1E-30
score: 107.2
IPR021109Aspartic peptidase domain superfamilyGENE3D2.40.70.10Acid Proteasescoord: 1..156
e-value: 5.8E-29
score: 103.4
IPR021109Aspartic peptidase domain superfamilyGENE3D2.40.70.10Acid Proteasescoord: 157..351
e-value: 4.1E-37
score: 129.5
IPR021109Aspartic peptidase domain superfamilySUPERFAMILY50630Acid proteasescoord: 2..347
IPR032799Xylanase inhibitor, C-terminalPFAMPF14541TAXi_Ccoord: 185..342
e-value: 8.6E-32
score: 110.1
NoneNo IPR availablePANTHERPTHR47965:SF51EUKARYOTIC ASPARTYL PROTEASE FAMILY PROTEINcoord: 2..348
IPR033121Peptidase family A1 domainPROSITEPS51767PEPTIDASE_A1coord: 1..343
score: 25.082338
IPR034161Pepsin-like domain, plantCDDcd05476pepsin_A_like_plantcoord: 2..347
e-value: 2.43361E-63
score: 200.567

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh01G003500.1CmoCh01G003500.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
molecular_function GO:0004190 aspartic-type endopeptidase activity