CmoCh01G001560 (gene) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh01G001560
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
DescriptionDelta 9 desaturase
LocationCmo_Chr01: 665595 .. 668861 (-)
RNA-Seq ExpressionCmoCh01G001560
SyntenyCmoCh01G001560
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGTGAAGTCCAAACCTGCTCTTCTAAACAGGAAATGGACCATTTTAGACAGACGTTCGGCTGGTGGTGTTTTGTTCTTGCATTTCCTCTGTCTCTTGGCTCCATTTCACTGCAACTGGCCAGCCTTTTGGGTTGCCTTTGCACTCTATTTCATTACAGGGCTTTTCGGAGTAACGCTATCGTACCATAGAAATCTTGCACACAAAAGTTTCAAGCTTCCCAAATGGCTCGAGTACTTGTTTGCATATCTTGGAGTTCAGGCCTTCCAGGTTTGTCGTGATTATCGTGTCATGCATTAAAAACATTAAAAGAGTTAACAAACAAGCTTGTAGTGATTTGAAGTTTGCTAAATTTTACTACAGGGTGATCCAATTGATTGGGTTAGTACACATAGATATCATCATCAATTCGTTGATACAGACAGAGATCCACATAGTCCTTTAGATGGGTTTTGGTTTAGTCATTTAGCTTGGATGTTCGACGCGTATACTTTGACAAAAAAAGTTTACCCAAAAAACGTTCAAGATTTCGAGGAAAGAAGTACCAAACGAAAGCGTTTTAGAAATGTTTTAGTGGGATATGTGAAGTATGGAAGACCAAACAATGTGGAGGATTTACAAAAGCAAGGCTTTTATAGGTTTCTTCGAAAGACATACATTCTCCATCCCACAGCTCCTGCAATTGTCCTCTATGCAACTGGAGGCCTTCCCTTTGTTGTTTGGGGAATGGTAACTATTGTTTTTTTCTATTTTTTTTTTATGTTCACATGATTTCTTCTTCTTTACTATTATTATTTGTAAGAATATAATAACAGAGTGGTTGGAGATGGGAACGAAACATTTCTTATGAGAGTGTGGAAACTTCCCTCTAACAGACGTGTTTTAAAACTGTGAGACTGATGGCGATACGTAACGGTCCAAAGCGGACAATATCGGCTAGCGGTGGGCTTGAACTATTACAAATGATATCAAACCCAGACACCGAGCGGTGTGCCAACAAGGACATTGGGTCTTCAATGGGGGTGGATTGGGAGATCCCACATTTGTTGAAGAGGGGAATGAAATATTCCTTGTAAGGGTGTGGAAACCTCTCCCTAACACACACGTTTTTAAATCGTGAGGCTGACGATAATACGTAATGGGCCAAAGCGGACAATATCTGGTAGCAGTAGGTTTGAGATATTATAAATGGTATCAAAGCCAGACACTGGAAAGTGTATCAACAAGGACGCGTAGCCCCAACGGGATGGATTGTGAGATCCCACATTAGTTGGAGAGGAGAATGAAACATTTTTTAGAAAGACGTGGAAACCTCTCCATCATGAGATTGACGACGTGTTATATAGTAGCGGTGGAGTTGAATTGTTATATTTGTTTTGTTGAAAGGAGATGGTAAGAAAATTGATGAGTGTTAATGGTGAATGGGATGTGGCAGGGAGTGAGGCTTGTTTGGGTGCTGCACATTACTTTCTTCGTAAATTCTGCCTGCCACATATGGGGCTACCGACTGTGGGACACAAATGATTTGTCGAAGAACAATTGGTACACTCACTTTCTCTGTTCTTCCGTAGTTACTGCTTCAAATTCAATTGGTTTATAGATATGTGTAAAAGATTGTAAATGTTGGCAGGGTGATAGGGTTGCTTGCCTTTGGGGAGGGTTGGCACAACAACCACCATGCTTTTGAATATTCAGCTCGACATGGCTATGAATGGTGGGAGTTTGACTTGACATGGTGCGTTATTTTGTTCCTTCAAGCCCTTGGATTGGCAACTGATGTGAAGTTACCCTCCGCACGCCATCAGCAAAAATTAGCCCTAAACCCTAAAACAAGCTGATGATTTCTAGACACTTGTTTCATGCTAGGCATTATTCCTCCAATGATTGTAGTATCAAGTATTTTTTGTGAGGCCTGCACGATGGGTTATTAACTCAACGGCAGAGCGCGCCTCTAATAATTTCATTGATGATTTCTTAGAGACTTGTTTGGTGCATTGATTGGATTAAACATAGTTTGCAAGTTTGATAATATTGTTAAAGTTATGTTATACCAAATTTCCTTAAATTCGAGTGAGTTTGAACGATGTGTAACATCCAAATAAAATAGGAATATATGAATGAACTGATACTATATATTCAAAGGAGGGCAATCCTAAGGATAAGATGACTAAGAAAAACTTAAAAGAATTAAAGGTTAATATCTATACCAATAATGTGCACGTTCCTTTTCGGTGTCGCAATGATAAGAACTTCATGATTAAGCATGCTTTACTTTGAATAATTCTATATTGGATGATCTCTTGATAATTTTCCTTAGATACACATGAGTGAGGATAAAACACGATGGAAGAACCTGCGTTGGTCTATTGGGATAGTCTATACTCTTAATAAGCGATGAATAGGTAACATGTGCACAGGTGTTGAGATCATCAGGTGCCCAAATCCTGATATGAATCCTAGGCATAAGGTCGTTGCATTATGTCCATAGACTTATCATATGTCACACCAATTATTCAAAAATTTAGAAGATGAATGGAAACTTACTCTAAACTATAATTTAACCTAATCTTATTATATAATACAAATTAATTGGAGATAATTGATTTGAAATTATGGTACCCAAATTAAATGTCAAAAGTAGGGCAACTATTAGGTGCAATAATGTGTTCTTGGGAAAGCGAAGCTTTTGTGGCCTTAAAGAGCCATTGCTATATCACATCATGTGGGTACCATGCTTTCAATCTTTGCATGCGACGCCCTCTGTTTGGGGGTAGGACTCATTTCAATTTAATTACGTATCATCACTAGAATTCTGTTCAACTAATTTGAACTGCGATCGTGATGGATTTAAGTCAATTCTATTAAACGTCACGAGTGAAGACTATGCATATAAACCAACACGTGCATCACCTCTGACATGGTCAAATTATCCAATCCTTACTTCTAAGAGTGAAAATTACCCCACAAATTAACTATAAAGTTTCTATTATTGAAACATCGAAAATGAGGGTGCACCTTGTTAGTATGTGTAAACTAGTCATTCTATTCTCAAAATCGTTTTTGAGAAGCAAAAACGGGTGGAGAGAAGCAGGCCAGAACTCGAACCTGAGAACGGTGGGCATGGATGCTTGGAACCAACAATTGGTAAACAGCGGAGGGTTGAGGCGTCGGAAACAAGCGGCGGAGGAAGATCACAAAGCGGCGGAGGAAGATCACAAAACTCAGAAAATGATTAAGAGGAGGCAGGAATGGTTGATGGTTAGATAA

mRNA sequence

ATGGTGAAGTCCAAACCTGCTCTTCTAAACAGGAAATGGACCATTTTAGACAGACGTTCGGCTGGTGGTGTTTTGTTCTTGCATTTCCTCTGTCTCTTGGCTCCATTTCACTGCAACTGGCCAGCCTTTTGGGTTGCCTTTGCACTCTATTTCATTACAGGGCTTTTCGGAGTAACGCTATCGTACCATAGAAATCTTGCACACAAAAGTTTCAAGCTTCCCAAATGGCTCGAGTACTTGTTTGCATATCTTGGAGTTCAGGCCTTCCAGGGTGATCCAATTGATTGGGTTAGTACACATAGATATCATCATCAATTCGTTGATACAGACAGAGATCCACATAGTCCTTTAGATGGGTTTTGGTTTAGTCATTTAGCTTGGATGTTCGACGCGTATACTTTGACAAAAAAAGTTTACCCAAAAAACGTTCAAGATTTCGAGGAAAGAAGTACCAAACGAAAGCGTTTTAGAAATGTTTTAGTGGGATATGTGAAGTATGGAAGACCAAACAATGTGGAGGATTTACAAAAGCAAGGCTTTTATAGGTTTCTTCGAAAGACATACATTCTCCATCCCACAGCTCCTGCAATTGTCCTCTATGCAACTGGAGGCCTTCCCTTTGTTGTTTGGGGAATGGGAGTGAGGCTTGTTTGGGTGCTGCACATTACTTTCTTCGTAAATTCTGCCTGCCACATATGGGGCTACCGACTGTGGGACACAAATGATTTGTCGAAGAACAATTGGGTGATAGGGTTGCTTGCCTTTGGGGAGGGTTGGCACAACAACCACCATGCTTTTGAATATTCAGCTCGACATGGCTATGAATGGTGGGAGTTTGACTTGACATGCGGAGGGTTGAGGCGTCGGAAACAAGCGGCGGAGGAAGATCACAAAGCGGCGGAGGAAGATCACAAAACTCAGAAAATGATTAAGAGGAGGCAGGAATGGTTGATGGTTAGATAA

Coding sequence (CDS)

ATGGTGAAGTCCAAACCTGCTCTTCTAAACAGGAAATGGACCATTTTAGACAGACGTTCGGCTGGTGGTGTTTTGTTCTTGCATTTCCTCTGTCTCTTGGCTCCATTTCACTGCAACTGGCCAGCCTTTTGGGTTGCCTTTGCACTCTATTTCATTACAGGGCTTTTCGGAGTAACGCTATCGTACCATAGAAATCTTGCACACAAAAGTTTCAAGCTTCCCAAATGGCTCGAGTACTTGTTTGCATATCTTGGAGTTCAGGCCTTCCAGGGTGATCCAATTGATTGGGTTAGTACACATAGATATCATCATCAATTCGTTGATACAGACAGAGATCCACATAGTCCTTTAGATGGGTTTTGGTTTAGTCATTTAGCTTGGATGTTCGACGCGTATACTTTGACAAAAAAAGTTTACCCAAAAAACGTTCAAGATTTCGAGGAAAGAAGTACCAAACGAAAGCGTTTTAGAAATGTTTTAGTGGGATATGTGAAGTATGGAAGACCAAACAATGTGGAGGATTTACAAAAGCAAGGCTTTTATAGGTTTCTTCGAAAGACATACATTCTCCATCCCACAGCTCCTGCAATTGTCCTCTATGCAACTGGAGGCCTTCCCTTTGTTGTTTGGGGAATGGGAGTGAGGCTTGTTTGGGTGCTGCACATTACTTTCTTCGTAAATTCTGCCTGCCACATATGGGGCTACCGACTGTGGGACACAAATGATTTGTCGAAGAACAATTGGGTGATAGGGTTGCTTGCCTTTGGGGAGGGTTGGCACAACAACCACCATGCTTTTGAATATTCAGCTCGACATGGCTATGAATGGTGGGAGTTTGACTTGACATGCGGAGGGTTGAGGCGTCGGAAACAAGCGGCGGAGGAAGATCACAAAGCGGCGGAGGAAGATCACAAAACTCAGAAAATGATTAAGAGGAGGCAGGAATGGTTGATGGTTAGATAA

Protein sequence

MVKSKPALLNRKWTILDRRSAGGVLFLHFLCLLAPFHCNWPAFWVAFALYFITGLFGVTLSYHRNLAHKSFKLPKWLEYLFAYLGVQAFQGDPIDWVSTHRYHHQFVDTDRDPHSPLDGFWFSHLAWMFDAYTLTKKVYPKNVQDFEERSTKRKRFRNVLVGYVKYGRPNNVEDLQKQGFYRFLRKTYILHPTAPAIVLYATGGLPFVVWGMGVRLVWVLHITFFVNSACHIWGYRLWDTNDLSKNNWVIGLLAFGEGWHNNHHAFEYSARHGYEWWEFDLTCGGLRRRKQAAEEDHKAAEEDHKTQKMIKRRQEWLMVR
Homology
BLAST of CmoCh01G001560 vs. ExPASy Swiss-Prot
Match: Q949X0 (Palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ADS3 PE=1 SV=2)

HSP 1 Score: 384.4 bits (986), Expect = 1.2e-105
Identity = 174/280 (62.14%), Postives = 201/280 (71.79%), Query Frame = 0

Query: 3   KSKPALLNRKWTILDRRSAGGVLFLHFLCLLAPFHCNWPAFWVAFALYFITGLFGVTLSY 62
           K K     R+W  +D  +   VL +H L LLAPF  NW A  VAF LY +TGL G+TLS+
Sbjct: 88  KEKVVWWEREWKAMDFGAVAVVLSMHLLSLLAPFQFNWRAVSVAFGLYIVTGLLGITLSF 147

Query: 63  HRNLAHKSFKLPKWLEYLFAYLGVQAFQGDPIDWVSTHRYHHQFVDTDRDPHSPLDGFWF 122
           HRNL+HK+FKLPKWLEYLFAY G QA QG+PIDWVSTHRYHHQF D+DRDPHSPLDGFWF
Sbjct: 148 HRNLSHKAFKLPKWLEYLFAYCGAQALQGNPIDWVSTHRYHHQFCDSDRDPHSPLDGFWF 207

Query: 123 SHLAWMFDAYTLTKKVYPKNVQDFEERSTKRKRFRNVLVGYVKYGRPNNVEDLQKQGFYR 182
           SH+ WMFD  T+T++                             G PNNV DL+KQ FYR
Sbjct: 208 SHMNWMFDTNTITQRC----------------------------GEPNNVGDLEKQPFYR 267

Query: 183 FLRKTYILHPTAPAIVLYATGGLPFVVWGMGVRLVWVLHITFFVNSACHIWGYRLWDTND 242
           FLR TYILHP A A+ LYA GG PF+VWGMGVR+VWV HIT+ VNSACH+WG + W+T D
Sbjct: 268 FLRTTYILHPLALAVALYAMGGFPFIVWGMGVRIVWVYHITWLVNSACHVWGKQAWNTGD 327

Query: 243 LSKNNWVIGLLAFGEGWHNNHHAFEYSARHGYEWWEFDLT 283
           LSKNNW +  LAFGEGWHNNHHAFE+SARHG EWW+ D+T
Sbjct: 328 LSKNNWWVAALAFGEGWHNNHHAFEFSARHGLEWWQLDMT 339

BLAST of CmoCh01G001560 vs. ExPASy Swiss-Prot
Match: O65797 (Delta-9 acyl-lipid desaturase 1 OS=Arabidopsis thaliana OX=3702 GN=ADS1 PE=2 SV=1)

HSP 1 Score: 321.2 bits (822), Expect = 1.3e-86
Identity = 148/282 (52.48%), Postives = 184/282 (65.25%), Query Frame = 0

Query: 1   MVKSKPALLNRKWTILDRRSAGGVLFLHFLCLLAPFHCNWPAFWVAFALYFITGLFGVTL 60
           M + K A+  RKW  LD   A   LF+HFLCLLAPF+  WPA  VA  +Y + GL G+T+
Sbjct: 22  MGRKKRAMWERKWKRLDIVKAFASLFVHFLCLLAPFNFTWPALRVALIVYTVGGL-GITV 81

Query: 61  SYHRNLAHKSFKLPKWLEYLFAYLGVQAFQGDPIDWVSTHRYHHQFVDTDRDPHSPLDGF 120
           SYHRNLAH+SFK+PKWLEY FAY G+ A QGDPIDWVSTHRYHHQF D+DRDPHSP +GF
Sbjct: 82  SYHRNLAHRSFKVPKWLEYFFAYCGLLAIQGDPIDWVSTHRYHHQFTDSDRDPHSPNEGF 141

Query: 121 WFSHLAWMFDAYTLTKKVYPKNVQDFEERSTKRKRFRNVLVGYVKYGRPNNVEDLQKQGF 180
           WFSHL W+FD   L +K                             GR  NVEDL++Q +
Sbjct: 142 WFSHLLWLFDTGYLVEKC----------------------------GRRTNVEDLKRQWY 201

Query: 181 YRFLRKTYILHPTAPAIVLYATGGLPFVVWGMGVRLVWVLHITFFVNSACHIWGYRLWDT 240
           Y+FL++T + H      +LY  GGL F+ WGMG+ +    H+T  +NS CH+WG R W T
Sbjct: 202 YKFLQRTVLYHILTFGFLLYYFGGLSFLTWGMGIGVAMEHHVTCLINSLCHVWGSRTWKT 261

Query: 241 NDLSKNNWVIGLLAFGEGWHNNHHAFEYSARHGYEWWEFDLT 283
           ND S+N W + + +FGE WHNNHHAFE SAR G EWW+ D++
Sbjct: 262 NDTSRNVWWLSVFSFGESWHNNHHAFESSARQGLEWWQIDIS 274

BLAST of CmoCh01G001560 vs. ExPASy Swiss-Prot
Match: Q9LVZ3 (Probable lipid desaturase ADS3.2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ADS3.2 PE=2 SV=3)

HSP 1 Score: 315.1 bits (806), Expect = 9.3e-85
Identity = 145/282 (51.42%), Postives = 184/282 (65.25%), Query Frame = 0

Query: 1   MVKSKPALLNRKWTILDRRSAGGVLFLHFLCLLAPFHCNWPAFWVAFALYFITGLFGVTL 60
           +V++K +   R WT  D       +  H L LLAPF+ +W AFWV   L FI G+  +TL
Sbjct: 79  VVRTKRSFWERSWTSWDVSKLVIFVGTHLLSLLAPFYFSWEAFWVFPWLVFINGIC-ITL 138

Query: 61  SYHRNLAHKSFKLPKWLEYLFAYLGVQAFQGDPIDWVSTHRYHHQFVDTDRDPHSPLDGF 120
           SYHRNL+H+SF LPKWLEYLFAY GV AFQGDPI+WVS HRYHH+  +T RDPHSP  GF
Sbjct: 139 SYHRNLSHRSFDLPKWLEYLFAYGGVLAFQGDPIEWVSNHRYHHKHCETQRDPHSPTQGF 198

Query: 121 WFSHLAWMFDAYTLTKKVYPKNVQDFEERSTKRKRFRNVLVGYVKYGRPNNVEDLQKQGF 180
           WFSH+AW+FD  ++ +                              G   NV+DL +Q F
Sbjct: 199 WFSHMAWIFDTSSILENC----------------------------GGEENVDDLVRQPF 258

Query: 181 YRFLRKTYILHPTAPAIVLYATGGLPFVVWGMGVRLVWVLHITFFVNSACHIWGYRLWDT 240
           YRFL++T +LH  A + + Y  GG+P +VWG+G+ +   LH+TF VNS CHIWG R W+T
Sbjct: 259 YRFLQRTVLLHMMAYSFLFYFCGGMPLLVWGIGITIAVRLHLTFLVNSVCHIWGTRAWNT 318

Query: 241 NDLSKNNWVIGLLAFGEGWHNNHHAFEYSARHGYEWWEFDLT 283
           +D SKNNW + +L  GEGWHNNHHAFE+SARHG EWW+ D+T
Sbjct: 319 SDFSKNNWWVAILTLGEGWHNNHHAFEFSARHGLEWWQLDIT 331

BLAST of CmoCh01G001560 vs. ExPASy Swiss-Prot
Match: P0DOW3 (Acyl-CoA 5-desaturase AL21 OS=Anemone leveillei OX=212809 GN=AL21 PE=1 SV=1)

HSP 1 Score: 313.5 bits (802), Expect = 2.7e-84
Identity = 151/310 (48.71%), Postives = 188/310 (60.65%), Query Frame = 0

Query: 3   KSKPALLNRKWTILDRRSAGGVLFLHFLCLLAPFHCNWPAFWVAFALYFITGLFGVTLSY 62
           K+K     R W   D      V  LH LCL APF  +W AFW++  LY + G+FG TLSY
Sbjct: 27  KTKKITWVRVWNSTDIFHIILVGGLHVLCLSAPFTFSWSAFWLSLTLYAVCGVFGTTLSY 86

Query: 63  HRNLAHKSFKLPKWLEYLFAYLGVQAFQGDPIDWVSTHRYHHQFVDTDRDPHSPLDGFWF 122
           HRNL H+SFKLPK+LEY FAY+G+ A QGDP+ WVSTHRYHH+F DT  DPHSP++GFWF
Sbjct: 87  HRNLTHRSFKLPKYLEYFFAYVGLHALQGDPVWWVSTHRYHHKFTDTYLDPHSPIEGFWF 146

Query: 123 SHLAWMFDAYTLTKKVYPKNVQDFEERSTKRKRFRNVLVGYVKYGRPNNVEDLQKQGFYR 182
           SH+ W+FD+  + ++                             GR  N  DL KQ +YR
Sbjct: 147 SHIFWLFDSKYILEEC----------------------------GRYENAGDLLKQSYYR 206

Query: 183 FLRKTYILHPTAPAIVLYATGGLPFVVWGMGVRLVWVLHITFFVNSACHIWGYRLWDTND 242
           FL +T++ H    A +LY  GG PF+VWGM VR VW  H ++ VNS CH +G++ WDT D
Sbjct: 207 FLERTFVFHVYLQAALLYMFGGFPFIVWGMAVRTVWGFHASWLVNSVCHRYGHQAWDTGD 266

Query: 243 LSKNNWVIGLLAFGEGWHNNHHAFEYSARHGYEWWEFDLTCGGLRRRKQ-AAEEDHKAAE 302
           LS NNW I +L  GEGWHNNHHAFEYSARHG EWW+ D T   ++  +      D K   
Sbjct: 267 LSTNNWFIAMLTSGEGWHNNHHAFEYSARHGIEWWQIDTTWYVIKLLEYLGLATDVKVPS 308

Query: 303 EDHKTQKMIK 312
           E HK +   K
Sbjct: 327 EVHKRKMSFK 308

BLAST of CmoCh01G001560 vs. ExPASy Swiss-Prot
Match: P0DOW2 (Acyl-CoA C20 Delta5-desaturase OS=Anemone leveillei OX=212809 GN=AL10 PE=1 SV=1)

HSP 1 Score: 311.2 bits (796), Expect = 1.3e-83
Identity = 150/310 (48.39%), Postives = 187/310 (60.32%), Query Frame = 0

Query: 3   KSKPALLNRKWTILDRRSAGGVLFLHFLCLLAPFHCNWPAFWVAFALYFITGLFGVTLSY 62
           K+K     RKW   D      +  LH LCL AP   +W +FWV FALY I G+FG TLS+
Sbjct: 30  KTKKITWVRKWNSTDVFHILFIGGLHVLCLFAPSTFSWKSFWVCFALYAICGVFGTTLSF 89

Query: 63  HRNLAHKSFKLPKWLEYLFAYLGVQAFQGDPIDWVSTHRYHHQFVDTDRDPHSPLDGFWF 122
           HRNL H+SFKLPK+LEY FAY+G+ A QGDP+ WVSTHRYHH++ DT  DPHSP++GFWF
Sbjct: 90  HRNLTHRSFKLPKYLEYFFAYVGLHALQGDPVWWVSTHRYHHKYTDTYLDPHSPIEGFWF 149

Query: 123 SHLAWMFDAYTLTKKVYPKNVQDFEERSTKRKRFRNVLVGYVKYGRPNNVEDLQKQGFYR 182
            H+ W+FD+  + +K                             GR  N  DL KQ +YR
Sbjct: 150 CHIFWLFDSKYIIEKC----------------------------GRYENAGDLMKQSYYR 209

Query: 183 FLRKTYILHPTAPAIVLYATGGLPFVVWGMGVRLVWVLHITFFVNSACHIWGYRLWDTND 242
           FL +T++ H    A +LY  GG PF+VWGM VR +   H+++ VNS CH WG R W+T D
Sbjct: 210 FLERTFVYHVYLQAALLYLFGGFPFIVWGMAVRTILGFHLSWLVNSVCHRWGNRPWNTGD 269

Query: 243 LSKNNWVIGLLAFGEGWHNNHHAFEYSARHGYEWWEFDLTCGGLRRRKQ-AAEEDHKAAE 302
           LS NNW I +L  GEGWHNNHHAFEYSARHG EWW+ D T   ++  +      D K   
Sbjct: 270 LSTNNWFIAMLTSGEGWHNNHHAFEYSARHGIEWWQIDTTWYIIKLLEYLGLATDIKVPS 311

Query: 303 EDHKTQKMIK 312
           E HK +   K
Sbjct: 330 EIHKRKMSFK 311

BLAST of CmoCh01G001560 vs. ExPASy TrEMBL
Match: A0A6J1GCE1 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111452892 PE=3 SV=1)

HSP 1 Score: 616.3 bits (1588), Expect = 7.2e-173
Identity = 282/282 (100.00%), Postives = 282/282 (100.00%), Query Frame = 0

Query: 1   MVKSKPALLNRKWTILDRRSAGGVLFLHFLCLLAPFHCNWPAFWVAFALYFITGLFGVTL 60
           MVKSKPALLNRKWTILDRRSAGGVLFLHFLCLLAPFHCNWPAFWVAFALYFITGLFGVTL
Sbjct: 11  MVKSKPALLNRKWTILDRRSAGGVLFLHFLCLLAPFHCNWPAFWVAFALYFITGLFGVTL 70

Query: 61  SYHRNLAHKSFKLPKWLEYLFAYLGVQAFQGDPIDWVSTHRYHHQFVDTDRDPHSPLDGF 120
           SYHRNLAHKSFKLPKWLEYLFAYLGVQAFQGDPIDWVSTHRYHHQFVDTDRDPHSPLDGF
Sbjct: 71  SYHRNLAHKSFKLPKWLEYLFAYLGVQAFQGDPIDWVSTHRYHHQFVDTDRDPHSPLDGF 130

Query: 121 WFSHLAWMFDAYTLTKKVYPKNVQDFEERSTKRKRFRNVLVGYVKYGRPNNVEDLQKQGF 180
           WFSHLAWMFDAYTLTKKVYPKNVQDFEERSTKRKRFRNVLVGYVKYGRPNNVEDLQKQGF
Sbjct: 131 WFSHLAWMFDAYTLTKKVYPKNVQDFEERSTKRKRFRNVLVGYVKYGRPNNVEDLQKQGF 190

Query: 181 YRFLRKTYILHPTAPAIVLYATGGLPFVVWGMGVRLVWVLHITFFVNSACHIWGYRLWDT 240
           YRFLRKTYILHPTAPAIVLYATGGLPFVVWGMGVRLVWVLHITFFVNSACHIWGYRLWDT
Sbjct: 191 YRFLRKTYILHPTAPAIVLYATGGLPFVVWGMGVRLVWVLHITFFVNSACHIWGYRLWDT 250

Query: 241 NDLSKNNWVIGLLAFGEGWHNNHHAFEYSARHGYEWWEFDLT 283
           NDLSKNNWVIGLLAFGEGWHNNHHAFEYSARHGYEWWEFDLT
Sbjct: 251 NDLSKNNWVIGLLAFGEGWHNNHHAFEYSARHGYEWWEFDLT 292

BLAST of CmoCh01G001560 vs. ExPASy TrEMBL
Match: A0A6J1DIZ7 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS=Momordica charantia OX=3673 GN=LOC111020970 PE=3 SV=1)

HSP 1 Score: 545.0 bits (1403), Expect = 2.0e-151
Identity = 252/306 (82.35%), Postives = 268/306 (87.58%), Query Frame = 0

Query: 1   MVKSKPALLNRKWTILDRRSAGGVLFLHFLCLLAPFHCNWPAFWVAFALYFITGLFGVTL 60
           +VK +  LL RKWTILDRRSAG +LFLH L LLAPFHCNW AFWVAFALYFITGLFG+TL
Sbjct: 20  VVKPRRPLLKRKWTILDRRSAGAILFLHLLSLLAPFHCNWAAFWVAFALYFITGLFGITL 79

Query: 61  SYHRNLAHKSFKLPKWLEYLFAYLGVQAFQGDPIDWVSTHRYHHQFVDTDRDPHSPLDGF 120
           SYHRNLAHKSFKLPKWLEY FAYLGV AFQGDPIDWVSTHRYHH+FVDT+RDPHSPL+GF
Sbjct: 80  SYHRNLAHKSFKLPKWLEYAFAYLGVHAFQGDPIDWVSTHRYHHRFVDTERDPHSPLEGF 139

Query: 121 WFSHLAWMFDAYTLTKKVYPKNVQDFEERSTKRKRFRNVLVGYVKYGRPNNVEDLQKQGF 180
           WFSH+ WMFDAY LT+KV PKNVQDF+ER  KRKRF N+ VG+VKYGRPNNVEDLQKQ F
Sbjct: 140 WFSHIIWMFDAYALTEKVCPKNVQDFKERDAKRKRFSNIFVGFVKYGRPNNVEDLQKQAF 199

Query: 181 YRFLRKTYILHPTAPAIVLYATGGLPFVVWGMGVRLVWVLHITFFVNSACHIWGYRLWDT 240
           YRFLRKTYILHPTA AIVLYATGGLPF+VWGMGVRLVWVLHITFFVNSACHIWGYR WDT
Sbjct: 200 YRFLRKTYILHPTALAIVLYATGGLPFLVWGMGVRLVWVLHITFFVNSACHIWGYRRWDT 259

Query: 241 NDLSKNNWVIGLLAFGEGWHNNHHAFEYSARHGYEWWEFDLT-CGGLRRRKQAAEEDHKA 300
           NDLSKNNW IGLLAFGEGWHNNHHAFEYSARHG +WWEFDLT C  L  +      D K 
Sbjct: 260 NDLSKNNWWIGLLAFGEGWHNNHHAFEYSARHGLQWWEFDLTWCVVLVLQALGLATDVKL 319

Query: 301 AEEDHK 306
             + HK
Sbjct: 320 PSQIHK 325

BLAST of CmoCh01G001560 vs. ExPASy TrEMBL
Match: A0A6J1DIX0 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS=Momordica charantia OX=3673 GN=LOC111020949 PE=3 SV=1)

HSP 1 Score: 542.0 bits (1395), Expect = 1.7e-150
Identity = 240/282 (85.11%), Postives = 258/282 (91.49%), Query Frame = 0

Query: 1   MVKSKPALLNRKWTILDRRSAGGVLFLHFLCLLAPFHCNWPAFWVAFALYFITGLFGVTL 60
           ++K +   L RKWTILDRRSAGG+LFLH LCLLAPFHCNW AFWVAFALYFITGLFG+TL
Sbjct: 24  VLKPRRGFLKRKWTILDRRSAGGILFLHLLCLLAPFHCNWAAFWVAFALYFITGLFGITL 83

Query: 61  SYHRNLAHKSFKLPKWLEYLFAYLGVQAFQGDPIDWVSTHRYHHQFVDTDRDPHSPLDGF 120
           SYHRNLAHKSFKLPKWLEYLFAYLG+ AFQGDPIDWVSTHRYHHQFVDT+RDPHSPL+GF
Sbjct: 84  SYHRNLAHKSFKLPKWLEYLFAYLGLHAFQGDPIDWVSTHRYHHQFVDTERDPHSPLEGF 143

Query: 121 WFSHLAWMFDAYTLTKKVYPKNVQDFEERSTKRKRFRNVLVGYVKYGRPNNVEDLQKQGF 180
           WFSH+ WMFDAYTLTKKV PKNV+DFEE+  KRKRF N+ V  VKYGRPNNV+DLQKQ F
Sbjct: 144 WFSHITWMFDAYTLTKKVCPKNVEDFEEKDAKRKRFSNIFVAQVKYGRPNNVDDLQKQAF 203

Query: 181 YRFLRKTYILHPTAPAIVLYATGGLPFVVWGMGVRLVWVLHITFFVNSACHIWGYRLWDT 240
           YRFLR+TYILHPTA A+VLYA GGLPFVVWGMGVRLVW LHITFF+NSACHIWGYR W+T
Sbjct: 204 YRFLRRTYILHPTALALVLYAMGGLPFVVWGMGVRLVWFLHITFFINSACHIWGYRRWNT 263

Query: 241 NDLSKNNWVIGLLAFGEGWHNNHHAFEYSARHGYEWWEFDLT 283
            DLSKNNWV GLLAFGEGWHNNHHAFEYSARHG +WWEFDLT
Sbjct: 264 TDLSKNNWVFGLLAFGEGWHNNHHAFEYSARHGLQWWEFDLT 305

BLAST of CmoCh01G001560 vs. ExPASy TrEMBL
Match: A0A0A0KWV4 (Delta 9 desaturase OS=Cucumis sativus OX=3659 GN=Csa_4G006140 PE=3 SV=1)

HSP 1 Score: 504.2 bits (1297), Expect = 4.0e-139
Identity = 223/282 (79.08%), Postives = 244/282 (86.52%), Query Frame = 0

Query: 1   MVKSKPALLNRKWTILDRRSAGGVLFLHFLCLLAPFHCNWPAFWVAFALYFITGLFGVTL 60
           MV  K A  NRKWT LDRR+A G+LFLH L LLAPFH NW AFWVAFALY ITG  G+TL
Sbjct: 20  MVNPKRAFFNRKWTTLDRRTAAGILFLHLLSLLAPFHFNWAAFWVAFALYLITGALGITL 79

Query: 61  SYHRNLAHKSFKLPKWLEYLFAYLGVQAFQGDPIDWVSTHRYHHQFVDTDRDPHSPLDGF 120
           SYHRNLAH+SFKLPKWLEY FAYLGVQ FQGDPIDWVSTHRYHHQFVDTDRDPHSP+ GF
Sbjct: 80  SYHRNLAHRSFKLPKWLEYFFAYLGVQGFQGDPIDWVSTHRYHHQFVDTDRDPHSPMQGF 139

Query: 121 WFSHLAWMFDAYTLTKKVYPKNVQDFEERSTKRKRFRNVLVGYVKYGRPNNVEDLQKQGF 180
           WFSH+ WMFD+Y LT+K+YPKN++DF+ER  KR   RN  VG++KYGRP NVEDLQKQGF
Sbjct: 140 WFSHMTWMFDSYGLTEKIYPKNLEDFKEREAKRTLSRNWFVGFLKYGRPKNVEDLQKQGF 199

Query: 181 YRFLRKTYILHPTAPAIVLYATGGLPFVVWGMGVRLVWVLHITFFVNSACHIWGYRLWDT 240
           YRFLRKTY+LHP A AIVLY  GGLPFVVWGMGVRL+ VLH   FVNSACHIWGYR W+T
Sbjct: 200 YRFLRKTYLLHPIALAIVLYKFGGLPFVVWGMGVRLICVLHAALFVNSACHIWGYRKWNT 259

Query: 241 NDLSKNNWVIGLLAFGEGWHNNHHAFEYSARHGYEWWEFDLT 283
           NDLSKNNW+IGL++FGEGWHNNHHAFEYSARHG +WWEFD T
Sbjct: 260 NDLSKNNWIIGLISFGEGWHNNHHAFEYSARHGLQWWEFDFT 301

BLAST of CmoCh01G001560 vs. ExPASy TrEMBL
Match: A0A6J1GVF3 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111457787 PE=3 SV=1)

HSP 1 Score: 498.0 bits (1281), Expect = 2.8e-137
Identity = 221/282 (78.37%), Postives = 244/282 (86.52%), Query Frame = 0

Query: 1   MVKSKPALLNRKWTILDRRSAGGVLFLHFLCLLAPFHCNWPAFWVAFALYFITGLFGVTL 60
           +V  K   LNRKWT LDRR+A G+LFLH L L APFH NWPAFWVAF LYF TG+ G+TL
Sbjct: 20  VVNPKRPFLNRKWTTLDRRNAAGILFLHVLGLFAPFHYNWPAFWVAFTLYFTTGVLGITL 79

Query: 61  SYHRNLAHKSFKLPKWLEYLFAYLGVQAFQGDPIDWVSTHRYHHQFVDTDRDPHSPLDGF 120
           SYHRNLAH+SFKLPKWLEY FAYLGVQAFQGDPIDWVSTHRYHHQFVDT+RDPHSPL+GF
Sbjct: 80  SYHRNLAHRSFKLPKWLEYSFAYLGVQAFQGDPIDWVSTHRYHHQFVDTERDPHSPLEGF 139

Query: 121 WFSHLAWMFDAYTLTKKVYPKNVQDFEERSTKRKRFRNVLVGYVKYGRPNNVEDLQKQGF 180
           WFSH+ WMFD+Y LT K+YPK ++DFEER  KR   RN  VG++KYGRP NVEDLQK+GF
Sbjct: 140 WFSHVTWMFDSYGLTNKIYPKYLEDFEERDAKRTLSRNWFVGFLKYGRPKNVEDLQKEGF 199

Query: 181 YRFLRKTYILHPTAPAIVLYATGGLPFVVWGMGVRLVWVLHITFFVNSACHIWGYRLWDT 240
           YRFLRKTY+LHP A A+VLY  GGLPFVVWGMGVRL+ VLH  FFVNSACHIWGYR W+T
Sbjct: 200 YRFLRKTYLLHPIALAMVLYGFGGLPFVVWGMGVRLICVLHAAFFVNSACHIWGYRKWNT 259

Query: 241 NDLSKNNWVIGLLAFGEGWHNNHHAFEYSARHGYEWWEFDLT 283
           NDLSKNNWVIGL++ GEGWHNNHHAFEYSARHG +WWEFD T
Sbjct: 260 NDLSKNNWVIGLVSCGEGWHNNHHAFEYSARHGLQWWEFDFT 301

BLAST of CmoCh01G001560 vs. NCBI nr
Match: XP_022949581.1 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like [Cucurbita moschata])

HSP 1 Score: 616.3 bits (1588), Expect = 1.5e-172
Identity = 282/282 (100.00%), Postives = 282/282 (100.00%), Query Frame = 0

Query: 1   MVKSKPALLNRKWTILDRRSAGGVLFLHFLCLLAPFHCNWPAFWVAFALYFITGLFGVTL 60
           MVKSKPALLNRKWTILDRRSAGGVLFLHFLCLLAPFHCNWPAFWVAFALYFITGLFGVTL
Sbjct: 11  MVKSKPALLNRKWTILDRRSAGGVLFLHFLCLLAPFHCNWPAFWVAFALYFITGLFGVTL 70

Query: 61  SYHRNLAHKSFKLPKWLEYLFAYLGVQAFQGDPIDWVSTHRYHHQFVDTDRDPHSPLDGF 120
           SYHRNLAHKSFKLPKWLEYLFAYLGVQAFQGDPIDWVSTHRYHHQFVDTDRDPHSPLDGF
Sbjct: 71  SYHRNLAHKSFKLPKWLEYLFAYLGVQAFQGDPIDWVSTHRYHHQFVDTDRDPHSPLDGF 130

Query: 121 WFSHLAWMFDAYTLTKKVYPKNVQDFEERSTKRKRFRNVLVGYVKYGRPNNVEDLQKQGF 180
           WFSHLAWMFDAYTLTKKVYPKNVQDFEERSTKRKRFRNVLVGYVKYGRPNNVEDLQKQGF
Sbjct: 131 WFSHLAWMFDAYTLTKKVYPKNVQDFEERSTKRKRFRNVLVGYVKYGRPNNVEDLQKQGF 190

Query: 181 YRFLRKTYILHPTAPAIVLYATGGLPFVVWGMGVRLVWVLHITFFVNSACHIWGYRLWDT 240
           YRFLRKTYILHPTAPAIVLYATGGLPFVVWGMGVRLVWVLHITFFVNSACHIWGYRLWDT
Sbjct: 191 YRFLRKTYILHPTAPAIVLYATGGLPFVVWGMGVRLVWVLHITFFVNSACHIWGYRLWDT 250

Query: 241 NDLSKNNWVIGLLAFGEGWHNNHHAFEYSARHGYEWWEFDLT 283
           NDLSKNNWVIGLLAFGEGWHNNHHAFEYSARHGYEWWEFDLT
Sbjct: 251 NDLSKNNWVIGLLAFGEGWHNNHHAFEYSARHGYEWWEFDLT 292

BLAST of CmoCh01G001560 vs. NCBI nr
Match: KAG6606803.1 (Palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 607.1 bits (1564), Expect = 9.0e-170
Identity = 279/282 (98.94%), Postives = 279/282 (98.94%), Query Frame = 0

Query: 1   MVKSKPALLNRKWTILDRRSAGGVLFLHFLCLLAPFHCNWPAFWVAFALYFITGLFGVTL 60
           MVKSKPALLNRKWTILDRRSAGGVLFLHFLCLLAPFHCNWPAFWVAFALYFITGLFGVTL
Sbjct: 11  MVKSKPALLNRKWTILDRRSAGGVLFLHFLCLLAPFHCNWPAFWVAFALYFITGLFGVTL 70

Query: 61  SYHRNLAHKSFKLPKWLEYLFAYLGVQAFQGDPIDWVSTHRYHHQFVDTDRDPHSPLDGF 120
           SYHRNLAHKSFKLPKWLEYLFAYLGVQAFQGDPIDWVSTHRYHHQFVDTDRDPHSPLDGF
Sbjct: 71  SYHRNLAHKSFKLPKWLEYLFAYLGVQAFQGDPIDWVSTHRYHHQFVDTDRDPHSPLDGF 130

Query: 121 WFSHLAWMFDAYTLTKKVYPKNVQDFEERSTKRKRFRNVLVGYVKYGRPNNVEDLQKQGF 180
           WFSHLAWMFDAY LTKKV PKNVQDFEERSTKRKRFRNVLVGYVKYGRPNNVEDLQKQGF
Sbjct: 131 WFSHLAWMFDAYALTKKVCPKNVQDFEERSTKRKRFRNVLVGYVKYGRPNNVEDLQKQGF 190

Query: 181 YRFLRKTYILHPTAPAIVLYATGGLPFVVWGMGVRLVWVLHITFFVNSACHIWGYRLWDT 240
           YRFLRKTYILHPTA AIVLYATGGLPFVVWGMGVRLVWVLHITFFVNSACHIWGYRLWDT
Sbjct: 191 YRFLRKTYILHPTALAIVLYATGGLPFVVWGMGVRLVWVLHITFFVNSACHIWGYRLWDT 250

Query: 241 NDLSKNNWVIGLLAFGEGWHNNHHAFEYSARHGYEWWEFDLT 283
           NDLSKNNWVIGLLAFGEGWHNNHHAFEYSARHGYEWWEFDLT
Sbjct: 251 NDLSKNNWVIGLLAFGEGWHNNHHAFEYSARHGYEWWEFDLT 292

BLAST of CmoCh01G001560 vs. NCBI nr
Match: XP_023525371.1 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 602.4 bits (1552), Expect = 2.2e-168
Identity = 277/282 (98.23%), Postives = 278/282 (98.58%), Query Frame = 0

Query: 1   MVKSKPALLNRKWTILDRRSAGGVLFLHFLCLLAPFHCNWPAFWVAFALYFITGLFGVTL 60
           MVKSKP+LLNRKWTILDRRSAGGVLFLHFL LLAPFHCNWPAFWVAFALYFITGLFGVTL
Sbjct: 11  MVKSKPSLLNRKWTILDRRSAGGVLFLHFLSLLAPFHCNWPAFWVAFALYFITGLFGVTL 70

Query: 61  SYHRNLAHKSFKLPKWLEYLFAYLGVQAFQGDPIDWVSTHRYHHQFVDTDRDPHSPLDGF 120
           SYHRNLAHKSFKLPKW EYLFAYLGVQAFQGDPIDWVSTHRYHHQFVDTDRDPHSPLDGF
Sbjct: 71  SYHRNLAHKSFKLPKWFEYLFAYLGVQAFQGDPIDWVSTHRYHHQFVDTDRDPHSPLDGF 130

Query: 121 WFSHLAWMFDAYTLTKKVYPKNVQDFEERSTKRKRFRNVLVGYVKYGRPNNVEDLQKQGF 180
           WFSHLAWMFDAYTLTKKV PKNVQDFEERSTKRKRFRNVLVGYVKYGRPNNVEDLQKQGF
Sbjct: 131 WFSHLAWMFDAYTLTKKVCPKNVQDFEERSTKRKRFRNVLVGYVKYGRPNNVEDLQKQGF 190

Query: 181 YRFLRKTYILHPTAPAIVLYATGGLPFVVWGMGVRLVWVLHITFFVNSACHIWGYRLWDT 240
           YRFLRKTYILHPTA AIVLYATGGLPFVVWGMGVRLVWVLHITFFVNSACHIWGYRLWDT
Sbjct: 191 YRFLRKTYILHPTALAIVLYATGGLPFVVWGMGVRLVWVLHITFFVNSACHIWGYRLWDT 250

Query: 241 NDLSKNNWVIGLLAFGEGWHNNHHAFEYSARHGYEWWEFDLT 283
           NDLSKNNWVIGLLAFGEGWHNNHHAFEYSARHGYEWWEFDLT
Sbjct: 251 NDLSKNNWVIGLLAFGEGWHNNHHAFEYSARHGYEWWEFDLT 292

BLAST of CmoCh01G001560 vs. NCBI nr
Match: XP_022153467.1 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like [Momordica charantia])

HSP 1 Score: 545.0 bits (1403), Expect = 4.2e-151
Identity = 252/306 (82.35%), Postives = 268/306 (87.58%), Query Frame = 0

Query: 1   MVKSKPALLNRKWTILDRRSAGGVLFLHFLCLLAPFHCNWPAFWVAFALYFITGLFGVTL 60
           +VK +  LL RKWTILDRRSAG +LFLH L LLAPFHCNW AFWVAFALYFITGLFG+TL
Sbjct: 20  VVKPRRPLLKRKWTILDRRSAGAILFLHLLSLLAPFHCNWAAFWVAFALYFITGLFGITL 79

Query: 61  SYHRNLAHKSFKLPKWLEYLFAYLGVQAFQGDPIDWVSTHRYHHQFVDTDRDPHSPLDGF 120
           SYHRNLAHKSFKLPKWLEY FAYLGV AFQGDPIDWVSTHRYHH+FVDT+RDPHSPL+GF
Sbjct: 80  SYHRNLAHKSFKLPKWLEYAFAYLGVHAFQGDPIDWVSTHRYHHRFVDTERDPHSPLEGF 139

Query: 121 WFSHLAWMFDAYTLTKKVYPKNVQDFEERSTKRKRFRNVLVGYVKYGRPNNVEDLQKQGF 180
           WFSH+ WMFDAY LT+KV PKNVQDF+ER  KRKRF N+ VG+VKYGRPNNVEDLQKQ F
Sbjct: 140 WFSHIIWMFDAYALTEKVCPKNVQDFKERDAKRKRFSNIFVGFVKYGRPNNVEDLQKQAF 199

Query: 181 YRFLRKTYILHPTAPAIVLYATGGLPFVVWGMGVRLVWVLHITFFVNSACHIWGYRLWDT 240
           YRFLRKTYILHPTA AIVLYATGGLPF+VWGMGVRLVWVLHITFFVNSACHIWGYR WDT
Sbjct: 200 YRFLRKTYILHPTALAIVLYATGGLPFLVWGMGVRLVWVLHITFFVNSACHIWGYRRWDT 259

Query: 241 NDLSKNNWVIGLLAFGEGWHNNHHAFEYSARHGYEWWEFDLT-CGGLRRRKQAAEEDHKA 300
           NDLSKNNW IGLLAFGEGWHNNHHAFEYSARHG +WWEFDLT C  L  +      D K 
Sbjct: 260 NDLSKNNWWIGLLAFGEGWHNNHHAFEYSARHGLQWWEFDLTWCVVLVLQALGLATDVKL 319

Query: 301 AEEDHK 306
             + HK
Sbjct: 320 PSQIHK 325

BLAST of CmoCh01G001560 vs. NCBI nr
Match: XP_022153442.1 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like [Momordica charantia])

HSP 1 Score: 542.0 bits (1395), Expect = 3.5e-150
Identity = 240/282 (85.11%), Postives = 258/282 (91.49%), Query Frame = 0

Query: 1   MVKSKPALLNRKWTILDRRSAGGVLFLHFLCLLAPFHCNWPAFWVAFALYFITGLFGVTL 60
           ++K +   L RKWTILDRRSAGG+LFLH LCLLAPFHCNW AFWVAFALYFITGLFG+TL
Sbjct: 24  VLKPRRGFLKRKWTILDRRSAGGILFLHLLCLLAPFHCNWAAFWVAFALYFITGLFGITL 83

Query: 61  SYHRNLAHKSFKLPKWLEYLFAYLGVQAFQGDPIDWVSTHRYHHQFVDTDRDPHSPLDGF 120
           SYHRNLAHKSFKLPKWLEYLFAYLG+ AFQGDPIDWVSTHRYHHQFVDT+RDPHSPL+GF
Sbjct: 84  SYHRNLAHKSFKLPKWLEYLFAYLGLHAFQGDPIDWVSTHRYHHQFVDTERDPHSPLEGF 143

Query: 121 WFSHLAWMFDAYTLTKKVYPKNVQDFEERSTKRKRFRNVLVGYVKYGRPNNVEDLQKQGF 180
           WFSH+ WMFDAYTLTKKV PKNV+DFEE+  KRKRF N+ V  VKYGRPNNV+DLQKQ F
Sbjct: 144 WFSHITWMFDAYTLTKKVCPKNVEDFEEKDAKRKRFSNIFVAQVKYGRPNNVDDLQKQAF 203

Query: 181 YRFLRKTYILHPTAPAIVLYATGGLPFVVWGMGVRLVWVLHITFFVNSACHIWGYRLWDT 240
           YRFLR+TYILHPTA A+VLYA GGLPFVVWGMGVRLVW LHITFF+NSACHIWGYR W+T
Sbjct: 204 YRFLRRTYILHPTALALVLYAMGGLPFVVWGMGVRLVWFLHITFFINSACHIWGYRRWNT 263

Query: 241 NDLSKNNWVIGLLAFGEGWHNNHHAFEYSARHGYEWWEFDLT 283
            DLSKNNWV GLLAFGEGWHNNHHAFEYSARHG +WWEFDLT
Sbjct: 264 TDLSKNNWVFGLLAFGEGWHNNHHAFEYSARHGLQWWEFDLT 305

BLAST of CmoCh01G001560 vs. TAIR 10
Match: AT3G15850.1 (fatty acid desaturase 5 )

HSP 1 Score: 384.4 bits (986), Expect = 8.8e-107
Identity = 174/280 (62.14%), Postives = 201/280 (71.79%), Query Frame = 0

Query: 3   KSKPALLNRKWTILDRRSAGGVLFLHFLCLLAPFHCNWPAFWVAFALYFITGLFGVTLSY 62
           K K     R+W  +D  +   VL +H L LLAPF  NW A  VAF LY +TGL G+TLS+
Sbjct: 88  KEKVVWWEREWKAMDFGAVAVVLSMHLLSLLAPFQFNWRAVSVAFGLYIVTGLLGITLSF 147

Query: 63  HRNLAHKSFKLPKWLEYLFAYLGVQAFQGDPIDWVSTHRYHHQFVDTDRDPHSPLDGFWF 122
           HRNL+HK+FKLPKWLEYLFAY G QA QG+PIDWVSTHRYHHQF D+DRDPHSPLDGFWF
Sbjct: 148 HRNLSHKAFKLPKWLEYLFAYCGAQALQGNPIDWVSTHRYHHQFCDSDRDPHSPLDGFWF 207

Query: 123 SHLAWMFDAYTLTKKVYPKNVQDFEERSTKRKRFRNVLVGYVKYGRPNNVEDLQKQGFYR 182
           SH+ WMFD  T+T++                             G PNNV DL+KQ FYR
Sbjct: 208 SHMNWMFDTNTITQRC----------------------------GEPNNVGDLEKQPFYR 267

Query: 183 FLRKTYILHPTAPAIVLYATGGLPFVVWGMGVRLVWVLHITFFVNSACHIWGYRLWDTND 242
           FLR TYILHP A A+ LYA GG PF+VWGMGVR+VWV HIT+ VNSACH+WG + W+T D
Sbjct: 268 FLRTTYILHPLALAVALYAMGGFPFIVWGMGVRIVWVYHITWLVNSACHVWGKQAWNTGD 327

Query: 243 LSKNNWVIGLLAFGEGWHNNHHAFEYSARHGYEWWEFDLT 283
           LSKNNW +  LAFGEGWHNNHHAFE+SARHG EWW+ D+T
Sbjct: 328 LSKNNWWVAALAFGEGWHNNHHAFEFSARHGLEWWQLDMT 339

BLAST of CmoCh01G001560 vs. TAIR 10
Match: AT1G06080.1 (delta 9 desaturase 1 )

HSP 1 Score: 321.2 bits (822), Expect = 9.2e-88
Identity = 148/282 (52.48%), Postives = 184/282 (65.25%), Query Frame = 0

Query: 1   MVKSKPALLNRKWTILDRRSAGGVLFLHFLCLLAPFHCNWPAFWVAFALYFITGLFGVTL 60
           M + K A+  RKW  LD   A   LF+HFLCLLAPF+  WPA  VA  +Y + GL G+T+
Sbjct: 22  MGRKKRAMWERKWKRLDIVKAFASLFVHFLCLLAPFNFTWPALRVALIVYTVGGL-GITV 81

Query: 61  SYHRNLAHKSFKLPKWLEYLFAYLGVQAFQGDPIDWVSTHRYHHQFVDTDRDPHSPLDGF 120
           SYHRNLAH+SFK+PKWLEY FAY G+ A QGDPIDWVSTHRYHHQF D+DRDPHSP +GF
Sbjct: 82  SYHRNLAHRSFKVPKWLEYFFAYCGLLAIQGDPIDWVSTHRYHHQFTDSDRDPHSPNEGF 141

Query: 121 WFSHLAWMFDAYTLTKKVYPKNVQDFEERSTKRKRFRNVLVGYVKYGRPNNVEDLQKQGF 180
           WFSHL W+FD   L +K                             GR  NVEDL++Q +
Sbjct: 142 WFSHLLWLFDTGYLVEKC----------------------------GRRTNVEDLKRQWY 201

Query: 181 YRFLRKTYILHPTAPAIVLYATGGLPFVVWGMGVRLVWVLHITFFVNSACHIWGYRLWDT 240
           Y+FL++T + H      +LY  GGL F+ WGMG+ +    H+T  +NS CH+WG R W T
Sbjct: 202 YKFLQRTVLYHILTFGFLLYYFGGLSFLTWGMGIGVAMEHHVTCLINSLCHVWGSRTWKT 261

Query: 241 NDLSKNNWVIGLLAFGEGWHNNHHAFEYSARHGYEWWEFDLT 283
           ND S+N W + + +FGE WHNNHHAFE SAR G EWW+ D++
Sbjct: 262 NDTSRNVWWLSVFSFGESWHNNHHAFESSARQGLEWWQIDIS 274

BLAST of CmoCh01G001560 vs. TAIR 10
Match: AT3G15870.1 (Fatty acid desaturase family protein )

HSP 1 Score: 315.1 bits (806), Expect = 6.6e-86
Identity = 145/282 (51.42%), Postives = 184/282 (65.25%), Query Frame = 0

Query: 1   MVKSKPALLNRKWTILDRRSAGGVLFLHFLCLLAPFHCNWPAFWVAFALYFITGLFGVTL 60
           +V++K +   R WT  D       +  H L LLAPF+ +W AFWV   L FI G+  +TL
Sbjct: 79  VVRTKRSFWERSWTSWDVSKLVIFVGTHLLSLLAPFYFSWEAFWVFPWLVFINGIC-ITL 138

Query: 61  SYHRNLAHKSFKLPKWLEYLFAYLGVQAFQGDPIDWVSTHRYHHQFVDTDRDPHSPLDGF 120
           SYHRNL+H+SF LPKWLEYLFAY GV AFQGDPI+WVS HRYHH+  +T RDPHSP  GF
Sbjct: 139 SYHRNLSHRSFDLPKWLEYLFAYGGVLAFQGDPIEWVSNHRYHHKHCETQRDPHSPTQGF 198

Query: 121 WFSHLAWMFDAYTLTKKVYPKNVQDFEERSTKRKRFRNVLVGYVKYGRPNNVEDLQKQGF 180
           WFSH+AW+FD  ++ +                              G   NV+DL +Q F
Sbjct: 199 WFSHMAWIFDTSSILENC----------------------------GGEENVDDLVRQPF 258

Query: 181 YRFLRKTYILHPTAPAIVLYATGGLPFVVWGMGVRLVWVLHITFFVNSACHIWGYRLWDT 240
           YRFL++T +LH  A + + Y  GG+P +VWG+G+ +   LH+TF VNS CHIWG R W+T
Sbjct: 259 YRFLQRTVLLHMMAYSFLFYFCGGMPLLVWGIGITIAVRLHLTFLVNSVCHIWGTRAWNT 318

Query: 241 NDLSKNNWVIGLLAFGEGWHNNHHAFEYSARHGYEWWEFDLT 283
           +D SKNNW + +L  GEGWHNNHHAFE+SARHG EWW+ D+T
Sbjct: 319 SDFSKNNWWVAILTLGEGWHNNHHAFEFSARHGLEWWQLDIT 331

BLAST of CmoCh01G001560 vs. TAIR 10
Match: AT2G31360.1 (16:0delta9 desaturase 2 )

HSP 1 Score: 305.4 bits (781), Expect = 5.2e-83
Identity = 141/273 (51.65%), Postives = 172/273 (63.00%), Query Frame = 0

Query: 10  NRKWTILDRRSAGGVLFLHFLCLLAPFHCNWPAFWVAFALYFITGLFGVTLSYHRNLAHK 69
           +R+W  LD         +H L LLAPF+  W A WV F  Y I GL G+T+SYHRNLAH+
Sbjct: 33  DRRWRRLDYVKFSASFTVHSLALLAPFYFTWSALWVTFLFYTIGGL-GITVSYHRNLAHR 92

Query: 70  SFKLPKWLEYLFAYLGVQAFQGDPIDWVSTHRYHHQFVDTDRDPHSPLDGFWFSHLAWMF 129
           SFK+PKWLEYL AY  + A QGDPIDWVSTHRYHHQF D++RDPHSP +GFWFSHL W++
Sbjct: 93  SFKVPKWLEYLLAYCALLAIQGDPIDWVSTHRYHHQFTDSERDPHSPKEGFWFSHLLWIY 152

Query: 130 DAYTLTKKVYPKNVQDFEERSTKRKRFRNVLVGYVKYGRPNNVEDLQKQGFYRFLRKTYI 189
           D+  L  K                             GR  NVEDL++Q FYRFL+KT +
Sbjct: 153 DSAYLVSKC----------------------------GRRANVEDLKRQWFYRFLQKTVL 212

Query: 190 LHPTAPAIVLYATGGLPFVVWGMGVRLVWVLHITFFVNSACHIWGYRLWDTNDLSKNNWV 249
            H       L+  GG+ FV WGMGV     +H+T  +NS CHIWG R W TND S+N W 
Sbjct: 213 FHILGLGFFLFYLGGMSFVTWGMGVGAALEVHVTCLINSLCHIWGTRTWKTNDTSRNVWW 272

Query: 250 IGLLAFGEGWHNNHHAFEYSARHGYEWWEFDLT 283
           + + +FGE WHNNHHAFE SAR G EWW+ D++
Sbjct: 273 LSVFSFGESWHNNHHAFESSARQGLEWWQIDIS 276

BLAST of CmoCh01G001560 vs. TAIR 10
Match: AT1G06100.1 (Fatty acid desaturase family protein )

HSP 1 Score: 292.0 bits (746), Expect = 6.0e-79
Identity = 143/280 (51.07%), Postives = 175/280 (62.50%), Query Frame = 0

Query: 3   KSKPALLNRKWTILDRRSAGGVLFLHFLCLLAPFHCNWPAFWVAFALYFITGLFGVTLSY 62
           K K A + RKWT  D   A  V  +H LCLLAPF+  W AF     L  +T L  +T SY
Sbjct: 18  KEKRAYVLRKWTQFDVGRASTVGTVHLLCLLAPFNYKWEAFRFGIILAILTNLC-ITFSY 77

Query: 63  HRNLAHKSFKLPKWLEYLFAYLGVQAFQGDPIDWVSTHRYHHQFVDTDRDPHSPLDGFWF 122
           HRNL H+SFKLPKWLEY FAY  + A QGDP+DWVS HR+HHQF D+DRDPHSP++GFWF
Sbjct: 78  HRNLTHRSFKLPKWLEYPFAYSALLALQGDPLDWVSIHRFHHQFTDSDRDPHSPIEGFWF 137

Query: 123 SHLAWMFDAYTLTKKVYPKNVQDFEERSTKRKRFRNVLVGYVKYGRPNNVEDLQKQGFYR 182
           SH+ W+FD   + +K                             GR NNV DL++Q FYR
Sbjct: 138 SHVLWIFDTDYIREKC----------------------------GRRNNVMDLKQQWFYR 197

Query: 183 FLRKTYILHPTAPAIVLYATGGLPFVVWGMGVRLVWVLHITFFVNSACHIWGYRLWDTND 242
           FL+KT +LH  A   ++Y  GGLP++ W +G   V   H T+ VNSACHI G + W TND
Sbjct: 198 FLKKTLVLHILAFWTLIYLWGGLPYLTWTVGFGGVIGYHGTWLVNSACHICGSQAWQTND 257

Query: 243 LSKNNWVIGLLAFGEGWHNNHHAFEYSARHGYEWWEFDLT 283
            S+N W + LL  GE WHNNHHAFE SARHG EW++ D+T
Sbjct: 258 TSRNVWWLALLTMGESWHNNHHAFETSARHGLEWYQLDIT 268

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q949X01.2e-10562.14Palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic OS=Arab... [more]
O657971.3e-8652.48Delta-9 acyl-lipid desaturase 1 OS=Arabidopsis thaliana OX=3702 GN=ADS1 PE=2 SV=... [more]
Q9LVZ39.3e-8551.42Probable lipid desaturase ADS3.2, chloroplastic OS=Arabidopsis thaliana OX=3702 ... [more]
P0DOW32.7e-8448.71Acyl-CoA 5-desaturase AL21 OS=Anemone leveillei OX=212809 GN=AL21 PE=1 SV=1[more]
P0DOW21.3e-8348.39Acyl-CoA C20 Delta5-desaturase OS=Anemone leveillei OX=212809 GN=AL10 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1GCE17.2e-173100.00palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS... [more]
A0A6J1DIZ72.0e-15182.35palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS... [more]
A0A6J1DIX01.7e-15085.11palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS... [more]
A0A0A0KWV44.0e-13979.08Delta 9 desaturase OS=Cucumis sativus OX=3659 GN=Csa_4G006140 PE=3 SV=1[more]
A0A6J1GVF32.8e-13778.37palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS... [more]
Match NameE-valueIdentityDescription
XP_022949581.11.5e-172100.00palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like [C... [more]
KAG6606803.19.0e-17098.94Palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic, partia... [more]
XP_023525371.12.2e-16898.23palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like [C... [more]
XP_022153467.14.2e-15182.35palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like [M... [more]
XP_022153442.13.5e-15085.11palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like [M... [more]
Match NameE-valueIdentityDescription
AT3G15850.18.8e-10762.14fatty acid desaturase 5 [more]
AT1G06080.19.2e-8852.48delta 9 desaturase 1 [more]
AT3G15870.16.6e-8651.42Fatty acid desaturase family protein [more]
AT2G31360.15.2e-8351.6516:0delta9 desaturase 2 [more]
AT1G06100.16.0e-7951.07Fatty acid desaturase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 283..303
NoneNo IPR availablePANTHERPTHR11351:SF94BNAC05G37460D PROTEINcoord: 165..286
NoneNo IPR availablePANTHERPTHR11351:SF94BNAC05G37460D PROTEINcoord: 6..149
IPR015876Acyl-CoA desaturasePRINTSPR00075FACDDSATRASEcoord: 100..129
score: 50.88
coord: 63..83
score: 30.83
coord: 40..62
score: 18.99
IPR015876Acyl-CoA desaturasePANTHERPTHR11351ACYL-COA DESATURASEcoord: 6..149
coord: 165..286
IPR015876Acyl-CoA desaturaseCDDcd03505Delta9-FADS-likecoord: 40..282
e-value: 1.70655E-68
score: 209.72
IPR005804Fatty acid desaturase domainPFAMPF00487FA_desaturasecoord: 43..279
e-value: 5.3E-19
score: 69.1

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh01G001560.1CmoCh01G001560.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006633 fatty acid biosynthetic process
biological_process GO:0006629 lipid metabolic process
cellular_component GO:0005789 endoplasmic reticulum membrane
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0009979 16:0 monogalactosyldiacylglycerol desaturase activity
molecular_function GO:0016717 oxidoreductase activity, acting on paired donors, with oxidation of a pair of donors resulting in the reduction of molecular oxygen to two molecules of water