CmoCh01G001220 (gene) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh01G001220
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
Descriptionnitrate regulatory gene2 protein-like
LocationCmo_Chr01: 524493 .. 527342 (-)
RNA-Seq ExpressionCmoCh01G001220
SyntenyCmoCh01G001220
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGTTGTTCTCAGTCGAAGATTGATAATGAAGAAGCGGTAGCCCGTTGTAAAGAACGGAAGATTCATATGAAGGAAGCGGTGACGACTCGAAACGCTTTCGCCGCCGCGCACTCTGCCTATTCTATGTCGCTGAAAAATATTGGAGCTGTTTTAAGTGATTATGCCCATGGTGAGGTTGAAAATCCCCAATTTGTTTATGTATCTACTCAACCTAATTCTGCCATTACTTCCGCTCCCGCCCCTAGTGAACCTTTTCCGCCGCCGCCGCCTTTGCCTCCTTCTAATTTTCCTAATTCTCTTGAAAGGGCGGCCACCATGCCTGAGATGAATGTGCACAAATCTGATCTCAAGCCAGAATCGCCGATTATTGAGGAAGAGGACAAAAATGAAAATGAAGGCTCTGTTGGTGGGTTAAGGAGGAGGAGAAGCAACAAAAGTAAAAGGGATGAAGGGAGTAGCCGAAATAGAAATTCGGAGCTAAATGACAATTTGGCCAATGCATCGCCGCAAGTGCCGCCGCCGCCACAGTCTGAGAACCGGCACATTCCACCGCCCCCACAACAAAATTCAACTTACGATTATTTCTTCTCTATGGATTTACCCGTCTCGACTTTCTCTGAAGTTGATGAGGTATACATTAACAAAGAGGAGATTGATATAAAGCCCAAGGTAGTGGACAGTGACGATATTGATGAGCAGAGAAAAAGTGTAAAGGCCGAGACAGCGGAGCCATTGCTCGAGGAGCCGGTGGAGCCGCCTCCCTCCCTGCCTGCAGAACCTGCAACTGCCGTGGCGAAGAGCTCGAAGAAGGCGAACCAAGCAGGATCTATGGGGTCCACAGAGGGAAAGAGGATGGTTAAGCCAGATTTGAATTTGTTGCAGATATTTATAGATATTGATGATCATTTTCTCAAGTCTTGTGAGAGTGCTCGTGAAGTGTCCAAGATGCTTGAGGCAACACGATTACACTATCATTCAAACTTTGCTGATAATCGAGGTATCAGTCAAGCTTGTTGTTATATCTTTTCTTGAAAAGAATATGATGTACTTTTCTTTAGGTCACATCGACCACTCTGCCAGGGTGATGCGCGTTATTACATGGAATCGATCATTTAGGGGATTGCCTAATATGGATGATGGAAAAGATGATTCCTATGCAGAAGAACAAGAAACTCATGCAACCGTGTTAGATAAACTACTAGCTTGGGAAAAGAAGCTGTATGATGAAGTGAAGGTATATGGAATACTCTTCCTCCTATCTGAATATTTAAGCATCTCTTTTAGCTCCTCATTTTCACAAACAATCTTCTGACATGATATAATTTCATCCTTTTAATTCATGAAAAACGATGGAAGTTCATACCATTAGCAAAGATGTGATGTCTTTTTACCATTTTTAGTTGTATCTGTTCTAAAAATGCTGCTTTGTGATTCACAAGCGCTCTTGACACCATCTTTATGATCCTTGGAGCCTTGAGGTCGCCATTTATGCATGTTCCTAATTGCAACCTTGTTCTCTCTAAAGTAATAGACTAGGCAGGCACTATATGTGTTGCTTTTGATGACCCTTGATCTTACTGGAATAAATTTGTGTTTCTATGATGGGACTAAAAAACTTGTGATATTACGATTGACAAGGCAGGTGAAGTTATGAAATTTGAATACCAAAAGAAGGTTGCTACATTGAATAGACTGAAGAAACGAGATTCTAATGTAGAAGCATTGGAGAAAGCAAAAGCAGCAGTAAGTCATCTGCACACTAGATATATTGTTGACATGCAATCCTTGGATTCAACTGTCTCAGAGATTAGTCGTCTACGAGACGAACAGTTATACCCAAAACTTGTTCAGCTTGTTAATGGGTGAGTTGCGTGAGGATGCACATACATATAGTTGTTCATTATATTTTATTGTCGCTTATTGTCCCTCGTTAATATAAGTAAAATGATTGTACATGATTTTGTTGGTTAACATATTTCTACTTTAGATGCACGGCTTCGTGGTATGGTAGAGTATTGTTGACATTCGTATCAAACCATTTTTATGATTCTAGTGTTGTTGTTCCACAGGATGGCGACGGTGTGGGATACGATGCGAGCTCATCATGAAATGCAATTGAAGATCGTAAGTGCATTGAGATGGATGGATCTTTCTCAATCCCCAAAAGAAACTAGTACCCATCATTACGAGCGCACGGTTCAGCTGTGTGGTGTTGTGAGAGATTGGCATTCACAGTTTGAGAAGCTTGTGCGGTGTCAAAAAGATTACATTAAAGCCTTAAACAGTTGGTTGAAACAAAATGTAGTTCCTATAGAGAGTAGTTTGAAAGAGAAGGTTTCTTCTCCACCCAGAGCTCAAAGTCCTCCAATTCATAAACTCCTCCTCGCTTGGGACGATCAACTCGAGCGACTCCCAGATGAACATCTCAAAACTGCCATATTCACCTTTGGTGGTGTGATTAGTACCATTATGCTACAGCAGGATGACGAGAGGAAATTGATGTTAAAGTGGGAGGAGACCGAGAAAGAACTCGAGCGCAAGGAGCGACATTTTAACGACTGGCATTACAAATACCAGCAACGAAGAACGCCTGATGAGTTGGACCCTGAAAAGTCTGAAGATAACACTCAGGATTCCGTAGTTACGGAGAAGTTAATCGTGGTAGAGTCATTGAAAAGGAGATTAGAAGAGGAAAAGGAAACTCATGCGAAGCAATGCCTTCATGTGAGGGAGAAATCGTTGTTAAACCTTAAGAATCAGCTGCCAGAACTCTTCAGGGCTTTGTCAGAATTCTCTTTTTCTAGTTCGGAGATGTACAAGAACTTGAGATCGGTTTGTCAAGTCTAG

mRNA sequence

ATGGGTTGTTCTCAGTCGAAGATTGATAATGAAGAAGCGGTAGCCCGTTGTAAAGAACGGAAGATTCATATGAAGGAAGCGGTGACGACTCGAAACGCTTTCGCCGCCGCGCACTCTGCCTATTCTATGTCGCTGAAAAATATTGGAGCTGTTTTAAGTGATTATGCCCATGGTGAGGTTGAAAATCCCCAATTTGTTTATGTATCTACTCAACCTAATTCTGCCATTACTTCCGCTCCCGCCCCTAGTGAACCTTTTCCGCCGCCGCCGCCTTTGCCTCCTTCTAATTTTCCTAATTCTCTTGAAAGGGCGGCCACCATGCCTGAGATGAATGTGCACAAATCTGATCTCAAGCCAGAATCGCCGATTATTGAGGAAGAGGACAAAAATGAAAATGAAGGCTCTGTTGGTGGGTTAAGGAGGAGGAGAAGCAACAAAAGTAAAAGGGATGAAGGGAGTAGCCGAAATAGAAATTCGGAGCTAAATGACAATTTGGCCAATGCATCGCCGCAAGTGCCGCCGCCGCCACAGTCTGAGAACCGGCACATTCCACCGCCCCCACAACAAAATTCAACTTACGATTATTTCTTCTCTATGGATTTACCCGTCTCGACTTTCTCTGAAGTTGATGAGGTATACATTAACAAAGAGGAGATTGATATAAAGCCCAAGGTAGTGGACAGTGACGATATTGATGAGCAGAGAAAAAGTGTAAAGGCCGAGACAGCGGAGCCATTGCTCGAGGAGCCGGTGGAGCCGCCTCCCTCCCTGCCTGCAGAACCTGCAACTGCCGTGGCGAAGAGCTCGAAGAAGGCGAACCAAGCAGGATCTATGGGGTCCACAGAGGGAAAGAGGATGGTTAAGCCAGATTTGAATTTGTTGCAGATATTTATAGATATTGATGATCATTTTCTCAAGTCTTGTGAGAGTGCTCGTGAAGTGTCCAAGATGCTTGAGGCAACACGATTACACTATCATTCAAACTTTGCTGATAATCGAGGTCACATCGACCACTCTGCCAGGGTGATGCGCGTTATTACATGGAATCGATCATTTAGGGGATTGCCTAATATGGATGATGGAAAAGATGATTCCTATGCAGAAGAACAAGAAACTCATGCAACCGTGTTAGATAAACTACTAGCTTGGGAAAAGAAGCTGTATGATGAAGTGAAGGCAGGTGAAGTTATGAAATTTGAATACCAAAAGAAGGTTGCTACATTGAATAGACTGAAGAAACGAGATTCTAATGTAGAAGCATTGGAGAAAGCAAAAGCAGCAGTAAGTCATCTGCACACTAGATATATTGTTGACATGCAATCCTTGGATTCAACTGTCTCAGAGATTAGTCGTCTACGAGACGAACAGTTATACCCAAAACTTGTTCAGCTTGTTAATGGGATGGCGACGGTGTGGGATACGATGCGAGCTCATCATGAAATGCAATTGAAGATCGTAAGTGCATTGAGATGGATGGATCTTTCTCAATCCCCAAAAGAAACTAGTACCCATCATTACGAGCGCACGGTTCAGCTGTGTGGTGTTGTGAGAGATTGGCATTCACAGTTTGAGAAGCTTGTGCGGTGTCAAAAAGATTACATTAAAGCCTTAAACAGTTGGTTGAAACAAAATGTAGTTCCTATAGAGAGTAGTTTGAAAGAGAAGGTTTCTTCTCCACCCAGAGCTCAAAGTCCTCCAATTCATAAACTCCTCCTCGCTTGGGACGATCAACTCGAGCGACTCCCAGATGAACATCTCAAAACTGCCATATTCACCTTTGGTGGTGTGATTAGTACCATTATGCTACAGCAGGATGACGAGAGGAAATTGATGTTAAAGTGGGAGGAGACCGAGAAAGAACTCGAGCGCAAGGAGCGACATTTTAACGACTGGCATTACAAATACCAGCAACGAAGAACGCCTGATGAGTTGGACCCTGAAAAGTCTGAAGATAACACTCAGGATTCCGTAGTTACGGAGAAGTTAATCGTGGTAGAGTCATTGAAAAGGAGATTAGAAGAGGAAAAGGAAACTCATGCGAAGCAATGCCTTCATGTGAGGGAGAAATCGTTGTTAAACCTTAAGAATCAGCTGCCAGAACTCTTCAGGGCTTTGTCAGAATTCTCTTTTTCTAGTTCGGAGATGTACAAGAACTTGAGATCGGTTTGTCAAGTCTAG

Coding sequence (CDS)

ATGGGTTGTTCTCAGTCGAAGATTGATAATGAAGAAGCGGTAGCCCGTTGTAAAGAACGGAAGATTCATATGAAGGAAGCGGTGACGACTCGAAACGCTTTCGCCGCCGCGCACTCTGCCTATTCTATGTCGCTGAAAAATATTGGAGCTGTTTTAAGTGATTATGCCCATGGTGAGGTTGAAAATCCCCAATTTGTTTATGTATCTACTCAACCTAATTCTGCCATTACTTCCGCTCCCGCCCCTAGTGAACCTTTTCCGCCGCCGCCGCCTTTGCCTCCTTCTAATTTTCCTAATTCTCTTGAAAGGGCGGCCACCATGCCTGAGATGAATGTGCACAAATCTGATCTCAAGCCAGAATCGCCGATTATTGAGGAAGAGGACAAAAATGAAAATGAAGGCTCTGTTGGTGGGTTAAGGAGGAGGAGAAGCAACAAAAGTAAAAGGGATGAAGGGAGTAGCCGAAATAGAAATTCGGAGCTAAATGACAATTTGGCCAATGCATCGCCGCAAGTGCCGCCGCCGCCACAGTCTGAGAACCGGCACATTCCACCGCCCCCACAACAAAATTCAACTTACGATTATTTCTTCTCTATGGATTTACCCGTCTCGACTTTCTCTGAAGTTGATGAGGTATACATTAACAAAGAGGAGATTGATATAAAGCCCAAGGTAGTGGACAGTGACGATATTGATGAGCAGAGAAAAAGTGTAAAGGCCGAGACAGCGGAGCCATTGCTCGAGGAGCCGGTGGAGCCGCCTCCCTCCCTGCCTGCAGAACCTGCAACTGCCGTGGCGAAGAGCTCGAAGAAGGCGAACCAAGCAGGATCTATGGGGTCCACAGAGGGAAAGAGGATGGTTAAGCCAGATTTGAATTTGTTGCAGATATTTATAGATATTGATGATCATTTTCTCAAGTCTTGTGAGAGTGCTCGTGAAGTGTCCAAGATGCTTGAGGCAACACGATTACACTATCATTCAAACTTTGCTGATAATCGAGGTCACATCGACCACTCTGCCAGGGTGATGCGCGTTATTACATGGAATCGATCATTTAGGGGATTGCCTAATATGGATGATGGAAAAGATGATTCCTATGCAGAAGAACAAGAAACTCATGCAACCGTGTTAGATAAACTACTAGCTTGGGAAAAGAAGCTGTATGATGAAGTGAAGGCAGGTGAAGTTATGAAATTTGAATACCAAAAGAAGGTTGCTACATTGAATAGACTGAAGAAACGAGATTCTAATGTAGAAGCATTGGAGAAAGCAAAAGCAGCAGTAAGTCATCTGCACACTAGATATATTGTTGACATGCAATCCTTGGATTCAACTGTCTCAGAGATTAGTCGTCTACGAGACGAACAGTTATACCCAAAACTTGTTCAGCTTGTTAATGGGATGGCGACGGTGTGGGATACGATGCGAGCTCATCATGAAATGCAATTGAAGATCGTAAGTGCATTGAGATGGATGGATCTTTCTCAATCCCCAAAAGAAACTAGTACCCATCATTACGAGCGCACGGTTCAGCTGTGTGGTGTTGTGAGAGATTGGCATTCACAGTTTGAGAAGCTTGTGCGGTGTCAAAAAGATTACATTAAAGCCTTAAACAGTTGGTTGAAACAAAATGTAGTTCCTATAGAGAGTAGTTTGAAAGAGAAGGTTTCTTCTCCACCCAGAGCTCAAAGTCCTCCAATTCATAAACTCCTCCTCGCTTGGGACGATCAACTCGAGCGACTCCCAGATGAACATCTCAAAACTGCCATATTCACCTTTGGTGGTGTGATTAGTACCATTATGCTACAGCAGGATGACGAGAGGAAATTGATGTTAAAGTGGGAGGAGACCGAGAAAGAACTCGAGCGCAAGGAGCGACATTTTAACGACTGGCATTACAAATACCAGCAACGAAGAACGCCTGATGAGTTGGACCCTGAAAAGTCTGAAGATAACACTCAGGATTCCGTAGTTACGGAGAAGTTAATCGTGGTAGAGTCATTGAAAAGGAGATTAGAAGAGGAAAAGGAAACTCATGCGAAGCAATGCCTTCATGTGAGGGAGAAATCGTTGTTAAACCTTAAGAATCAGCTGCCAGAACTCTTCAGGGCTTTGTCAGAATTCTCTTTTTCTAGTTCGGAGATGTACAAGAACTTGAGATCGGTTTGTCAAGTCTAG

Protein sequence

MGCSQSKIDNEEAVARCKERKIHMKEAVTTRNAFAAAHSAYSMSLKNIGAVLSDYAHGEVENPQFVYVSTQPNSAITSAPAPSEPFPPPPPLPPSNFPNSLERAATMPEMNVHKSDLKPESPIIEEEDKNENEGSVGGLRRRRSNKSKRDEGSSRNRNSELNDNLANASPQVPPPPQSENRHIPPPPQQNSTYDYFFSMDLPVSTFSEVDEVYINKEEIDIKPKVVDSDDIDEQRKSVKAETAEPLLEEPVEPPPSLPAEPATAVAKSSKKANQAGSMGSTEGKRMVKPDLNLLQIFIDIDDHFLKSCESAREVSKMLEATRLHYHSNFADNRGHIDHSARVMRVITWNRSFRGLPNMDDGKDDSYAEEQETHATVLDKLLAWEKKLYDEVKAGEVMKFEYQKKVATLNRLKKRDSNVEALEKAKAAVSHLHTRYIVDMQSLDSTVSEISRLRDEQLYPKLVQLVNGMATVWDTMRAHHEMQLKIVSALRWMDLSQSPKETSTHHYERTVQLCGVVRDWHSQFEKLVRCQKDYIKALNSWLKQNVVPIESSLKEKVSSPPRAQSPPIHKLLLAWDDQLERLPDEHLKTAIFTFGGVISTIMLQQDDERKLMLKWEETEKELERKERHFNDWHYKYQQRRTPDELDPEKSEDNTQDSVVTEKLIVVESLKRRLEEEKETHAKQCLHVREKSLLNLKNQLPELFRALSEFSFSSSEMYKNLRSVCQV
Homology
BLAST of CmoCh01G001220 vs. ExPASy Swiss-Prot
Match: Q9AQW1 (Protein ROLLING AND ERECT LEAF 2 OS=Oryza sativa subsp. japonica OX=39947 GN=REL2 PE=2 SV=1)

HSP 1 Score: 225.7 bits (574), Expect = 1.7e-57
Identity = 225/775 (29.03%), Postives = 346/775 (44.65%), Query Frame = 0

Query: 1   MGCSQSKIDNEEAVARCKERKIHMKEAVTTRNAFAAAHSAYSMSLKNIGAVLSDYAHGEV 60
           MGC+ SK++ E+ V RCKER+ HMKEAV +R   A+AH+ Y  SL+   A LS +A G  
Sbjct: 1   MGCTASKVEQEDTVRRCKERRRHMKEAVASRQQLASAHADYLRSLRLTAAALSRFAQG-- 60

Query: 61  ENPQF-VYVSTQPNSAITSAPA-PSEPFPPPP--------PLPPSNFPNSLERAATMPEM 120
            +P   V   T P    T+APA    P PPPP        P P    P   +     P  
Sbjct: 61  -HPSLAVSHHTAPVLLTTAAPALAPTPTPPPPSSTASSSLPPPTPLLPKHQQAPPPPPPT 120

Query: 121 NVHKSDLKPESPIIEEEDKNENEGSVGGLRRRRSNKSKRDEGSSRNRNSELNDNLANASP 180
             H    +P  P+     +       GG RR +      D   +    S          P
Sbjct: 121 QSH----QPPPPVAVRAPR-------GGPRRLKVPHILSDSSVASPARSSFR------KP 180

Query: 181 QVPPPPQSE----NRHIPPPPQQNSTYDYFFSMDLPVSTFSEVDEV-----YINKEEIDI 240
            V  P  S         PP P  +  +D   +     +   E++E      Y++   +  
Sbjct: 181 VVGTPSSSSAWDWENFYPPSPPDSEFFDRRKADLEEANRLRELEEEEKARGYLHPHHLKE 240

Query: 241 KPKVVDSDD-----------IDEQRKSVKAETAEPLLEEPV-----------------EP 300
           + +V D DD            D+        T+E   EE                     
Sbjct: 241 EDEVDDDDDEREEEMHCGGWEDDDDHYASTTTSETRSEEGEMGNRSECGFAARSEYGGTA 300

Query: 301 PPSLPAEPATAVAKSSKKANQAGSMGST----EGKRMVKPDLNLLQIFIDIDDHFLKSCE 360
           P    A P     +   + ++AG   ST       RMV     L +I   I+++F+K+ E
Sbjct: 301 PSEYAAAPLPLPLRRRDERSEAGDSSSTVTAAAEMRMVIRHRTLAEIVAAIEEYFVKAAE 360

Query: 361 SAREVSKMLEATRLHYHSNFADNRGHIDHSARVMRVI--TWNRS--FRGLPNMDDGKDDS 420
           +   VS++LEA+R     NF   +  + HS  ++  +  TW           +D    + 
Sbjct: 361 AGNGVSELLEASRAQLDRNFRQLKKTVYHSNSLLSSLSSTWTSKPPLAVRYKLDTNALEM 420

Query: 421 YAEEQETHATVLDKLLAWEKKLYDEVKAGEVMKFEYQKKVATLNRLKKRDSNVEALEKAK 480
            + E ++H + L++LLAWEKKLY EVKA E +K E++KK++TL  L+ R  +   L+K K
Sbjct: 421 ESMEGKSHGSTLERLLAWEKKLYQEVKARESVKIEHEKKLSTLQSLEYRGRDSTKLDKTK 480

Query: 481 AAVSHLHTRYIVDMQSLDSTVSEISRLRDEQLYPKLVQLVNGMATVWDTMRAHHEMQLKI 540
           A+++ L +  IV  Q+  +T S I R+RD +L P+LV+L   + ++W +M   HE+Q +I
Sbjct: 481 ASINKLQSLIIVTSQAATTTSSAIVRVRDNELAPQLVELCFALLSMWRSMNHFHEIQNEI 540

Query: 541 VSALRWMDLSQSPKETSTHHYERTVQLCGVVRDWHSQFEKLVRCQKDYIKALNSWLKQNV 600
           V  +R +  +   + TS  H   T  L   V  WHS F +L++ Q+DYI+AL  WLK  +
Sbjct: 541 VQQVRGLVDNSMAESTSDLHRLATRDLEAAVSAWHSNFNRLIKYQRDYIRALYGWLKLTL 600

Query: 601 VPIESSLKEKVSSPPRAQSPPIHKLLLA----WDDQLERLPDEHLKTAIFTFGGVISTIM 660
             ++S++      P  A +  I + L      W   L+RLPD     AI +F  V+  I 
Sbjct: 601 FQVDSNI------PQEAYTSLISRELTTFCDEWKQALDRLPDASASEAIKSFVNVVHVIY 660

Query: 661 LQQDDERKLMLKWEETEKELERKERHFNDWHYKYQQRRTPDELD-PEKSEDNTQD----- 710
            +Q +E K+  + E   KELE+K         KY Q  +   L  P    D  +      
Sbjct: 661 TKQAEEMKIKKRTETYSKELEKKTNSLRAIEKKYYQSYSMVGLGLPGSGRDGIESHSFDA 720

BLAST of CmoCh01G001220 vs. ExPASy Swiss-Prot
Match: A0A178VBJ0 (Protein ALTERED PHOSPHATE STARVATION RESPONSE 1 OS=Arabidopsis thaliana OX=3702 GN=APSR1 PE=2 SV=1)

HSP 1 Score: 216.9 bits (551), Expect = 7.8e-55
Identity = 193/732 (26.37%), Postives = 314/732 (42.90%), Query Frame = 0

Query: 1   MGCSQSKIDNEEAVARCKERKIHMKEAVTTRNAFAAAHSAYSMSLKNIGAVLSDYAHGEV 60
           MGC QS+ID++E V+RCK RK ++K  V  R   + +H+ Y  SL+ +G+ L  ++  E 
Sbjct: 1   MGCCQSRIDSKEIVSRCKARKRYLKHLVKARQTLSVSHALYLRSLRAVGSSLVHFSSKET 60

Query: 61  ENPQFVYVSTQPNSAITSAPAPSEPFPPPPPLPPSNFPNSLERAATMPEMNVHKSDLKPE 120
                  +    N    S P P  P PPPPPL P +   +     T              
Sbjct: 61  P------LHLHHNPPSPSPPPPPPPRPPPPPLSPGSETTTWTTTTT-------------- 120

Query: 121 SPIIEEEDKNENEGSVGGLRRRRSNKSKRDEGSSRNRNSELNDNLANASPQVPPPPQSEN 180
                                                        ++  P  PPPP    
Sbjct: 121 ---------------------------------------------SSVLPPPPPPP---- 180

Query: 181 RHIPPPPQQNSTYDYFFSMDLPVSTFSEVDEVYINKEEIDIKPKVVDSDDIDEQRKSVKA 240
              PPPP  +ST+D++     P  + SE +     +EE     +       D       A
Sbjct: 181 ---PPPPPPSSTWDFWDPFIPPPPSSSEEEW----EEETTTATRTATGTGSD------AA 240

Query: 241 ETAEPLLEEPVEPPPSLPAEPATAVAKSSKKANQAGSMGSTEGKRMVKPDLNLLQIFIDI 300
            T  P    P         + ++ V+  SK      + GS     + +   +L++I  ++
Sbjct: 241 VTTAPTTATP---------QASSVVSGFSKDTMTTTTTGSELAVVVSRNGKDLMEIIKEV 300

Query: 301 DDHFLKSCESAREVSKMLE----ATRLHYHSNFADNRGHIDHSARVMRVITWNRSFRGLP 360
           D++FLK+ +S   +S +LE     T    HS         ++   +     W R F    
Sbjct: 301 DEYFLKAADSGAPLSSLLEISTSITDFSGHSKSGKMYSSSNYECNLNPTSFWTRGFA--- 360

Query: 361 NMDDGKDDSYAEE---------QETHATVLDKLLAWEKKLYDEVKAGEVMKFEYQKKVAT 420
                K   Y              +H++ +D+L AWEKKLY EVK  E +K +++KKV  
Sbjct: 361 ---PSKLSEYRNAGGVIGGNCIVGSHSSTVDRLYAWEKKLYQEVKYAESIKMDHEKKVEQ 420

Query: 421 LNRLKKRDSNVEALEKAKAAVSHLHTRYIVDMQSLDSTVSEISRLRDEQLYPKLVQLVNG 480
           + RL+ + +     EKAK  V  L ++  V  Q++ S  +EI +LR+ +LYP+LV+LV G
Sbjct: 421 VRRLEMKRAEYVKTEKAKKDVEKLESQLSVSSQAIQSASNEIIKLRETELYPQLVELVKG 480

Query: 481 MATVWDTMRAHHEMQLKIVSALRWMDLSQSPKETSTHHYERTVQLCGVVRDWHSQFEKLV 540
           +  +W +M   H++Q  IV  L++++   S + TS  H + T+QL   V+ WH  F  LV
Sbjct: 481 LMCMWRSMYESHQVQTHIVQQLKYLNTIPSTEPTSELHRQSTLQLELEVQQWHHSFCNLV 540

Query: 541 RCQKDYIKALNSWLKQNVVPIESSLKEKVSSPPRAQSPPIHKLLLAWDDQLERLPDEHLK 600
           + Q+DYI++L  WL+ ++     +   + S   +     I+     W   ++R+PD+   
Sbjct: 541 KAQRDYIQSLTGWLRLSLFQFSKNPLVRSSYESK-----IYSFCEEWHLAIDRIPDKVAS 600

Query: 601 TAIFTFGGVISTIMLQQDDERKLMLKWEETEKELERKERHFNDWHYKYQQRRTPDELDPE 660
             I +F   +  I+ QQ DE K   + E   K+ E+K         KY     P      
Sbjct: 601 EGIKSFLTAVHGIVAQQADEHKQKKRTESMLKDFEKKSASLRALESKYSPYSVP------ 621

Query: 661 KSEDNTQDSVVTEKLIVVESLKRRLEEEKETHAKQCLHVREKSLLNLKNQLPELFRALSE 720
              ++ + + V EK + VE LK + EEEK  H K     R  +L NL+   P +F+A+  
Sbjct: 661 ---ESRKKNPVIEKRVKVEMLKGKAEEEKSKHEKSVSVTRAMTLNNLQMGFPHVFQAMVG 621

BLAST of CmoCh01G001220 vs. ExPASy Swiss-Prot
Match: Q93YU8 (Nitrate regulatory gene2 protein OS=Arabidopsis thaliana OX=3702 GN=NRG2 PE=1 SV=1)

HSP 1 Score: 204.5 bits (519), Expect = 4.0e-51
Identity = 224/810 (27.65%), Postives = 363/810 (44.81%), Query Frame = 0

Query: 1   MGCSQSKIDNEEAVARCKERKIHMKEAVTTRNAFAAAHSAYSMSLKNIGAVLSDYAHGEV 60
           MGC+ SK+DNE+AV RCK+R+  MKEAV  R+  AAAH+ Y  SL+  G+ LS +A GE 
Sbjct: 1   MGCAASKLDNEDAVRRCKDRRRLMKEAVYARHHLAAAHADYCRSLRITGSALSSFASGEP 60

Query: 61  ENPQFVYVSTQPNSAITSAPAP--SEPFPP---PPPLPPSNFPNSLERAATMPEMNVHKS 120
                + VS Q  +     P P  SE  P    PP   PS  P+S+   +T P +   K 
Sbjct: 61  -----LSVSDQTPAVFLHTPPPPLSEQSPAKFVPPRFSPSPAPSSVYPPSTSPSVASSK- 120

Query: 121 DLKPESPIIEEEDKNENEGSVGGLRRRRSNKSKRDEGSSRNRNSELNDNL-----ANASP 180
                 P +     N         R            S R+  S    NL      N++ 
Sbjct: 121 -----QPSVMSTSSNRRRKQQPKPRLPHILSESSPSSSPRSERSNFMPNLYPSAYQNSTY 180

Query: 181 QVPPPPQS-----ENRHIPPPPQQ--------------------------NSTYDYFFSM 240
              P   S     EN + P PP                             S YD+F + 
Sbjct: 181 SATPSHASSVWNWENFYPPSPPDSEFFNRKAQEKKHNSDNRFNDEDTETVRSEYDFFDTR 240

Query: 241 DLPVSTFSEV-----DEVYINKEEIDI-------------KPKVVDSDDIDEQRKSV--- 300
                 F  +     +E    +EE+                    + ++ D+ R+S+   
Sbjct: 241 KQKQKQFESMRNQVEEETETEREEVQCSEWEDHDHYSTTSSSDAAEEEEEDDDRESISEV 300

Query: 301 --KAETAEPLLEEPV----EPPPSLPAEPATAVAKSSKKANQAG-SMGSTEG------KR 360
             ++E    +    +    + P  +P     A      KA+ A  S GS  G       +
Sbjct: 301 GTRSEFGSTVRSNSMRRHHQQPSPMPQVYGGAEQSKYDKADDATISSGSYRGGGDIADMK 360

Query: 361 MVKPDLNLLQIFIDIDDHFLKSCESAREVSKMLEATRLHYHSNFADNRGHIDHSARVMRV 420
           MV    +L +I   I ++F K+  S  +VS+MLE  R     +F+  +  + HS+ ++  
Sbjct: 361 MVVRHRDLKEIIDAIKENFDKAAASGEQVSQMLELGRAELDRSFSQLKKTVIHSSSLLSN 420

Query: 421 I--TWNRSFRGLPNMDDGKDDSYAEEQ----ETHATVLDKLLAWEKKLYDEVKAGEVMKF 480
           +  TW       P     + D+ A +Q    ++  + LD+LLAWEKKLY+E+KA E  K 
Sbjct: 421 LSSTWTSK---PPLAVKYRIDTTALDQPNSSKSLCSTLDRLLAWEKKLYEEIKAREGFKI 480

Query: 481 EYQKKVATLNRLKKRDSNVEALEKAKAAVSHLHTRYIVDMQSLDSTVSEISRLRDEQLYP 540
           E++KK++ L   + +  +   L+K KA+++ L +  IV  Q++ +T + I RLRD  L P
Sbjct: 481 EHEKKLSQLQSQEYKGEDEAKLDKTKASITRLQSLIIVTSQAVTTTSTAIIRLRDTDLVP 540

Query: 541 KLVQLVNGMATVWDTMRAHHEMQLKIVSALRWM-DLSQSPKETSTHHYERTVQLCGVVRD 600
           +LV+L +G   +W +M  +HE Q  IV  +R + + S   + TS  H + T  L   V  
Sbjct: 541 QLVELCHGFMYMWKSMHQYHETQNSIVEQVRGLINRSGKGESTSELHRQATRDLESAVSS 600

Query: 601 WHSQFEKLVRCQKDYIKALNSWLKQNVVPIESSLKEKVSSPPRAQSPPIHKLLLAWDDQL 660
           WHS F  L++ Q+D+I ++++W K  ++P+     ++ ++    +    +     W   L
Sbjct: 601 WHSSFSSLIKFQRDFIHSVHAWFKLTLLPV----CQEDAANHHKEPLDAYAFCDEWKLAL 660

Query: 661 ERLPDEHLKTAIFTFGGVISTIMLQQDDERKLMLKWEETEKELERKERHFNDWHYKYQQR 720
           +R+PD     AI +F  V+  I  +Q DE K+  + E   KELE+K     +   KY Q 
Sbjct: 661 DRIPDTVASEAIKSFINVVHVISAKQADEHKIKKRTESASKELEKKASSVRNLERKYYQS 720

Query: 721 RTPDELD-PEKSEDNTQ----DSVVTEKLIVVESLKRRLEEEKETHAKQCLHVREKSLLN 724
            +   +  PE   DN         +++K   +   +RR+EEE   ++K     R  +L N
Sbjct: 721 YSMVGVGLPESGPDNQHMLDARDPLSDKKSELAVCQRRVEEEMVKYSKAIEVTRAMTLNN 780

BLAST of CmoCh01G001220 vs. ExPASy TrEMBL
Match: A0A6J1G9I1 (nitrate regulatory gene2 protein-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111452183 PE=4 SV=1)

HSP 1 Score: 1404.4 bits (3634), Expect = 0.0e+00
Identity = 725/725 (100.00%), Postives = 725/725 (100.00%), Query Frame = 0

Query: 1   MGCSQSKIDNEEAVARCKERKIHMKEAVTTRNAFAAAHSAYSMSLKNIGAVLSDYAHGEV 60
           MGCSQSKIDNEEAVARCKERKIHMKEAVTTRNAFAAAHSAYSMSLKNIGAVLSDYAHGEV
Sbjct: 1   MGCSQSKIDNEEAVARCKERKIHMKEAVTTRNAFAAAHSAYSMSLKNIGAVLSDYAHGEV 60

Query: 61  ENPQFVYVSTQPNSAITSAPAPSEPFPPPPPLPPSNFPNSLERAATMPEMNVHKSDLKPE 120
           ENPQFVYVSTQPNSAITSAPAPSEPFPPPPPLPPSNFPNSLERAATMPEMNVHKSDLKPE
Sbjct: 61  ENPQFVYVSTQPNSAITSAPAPSEPFPPPPPLPPSNFPNSLERAATMPEMNVHKSDLKPE 120

Query: 121 SPIIEEEDKNENEGSVGGLRRRRSNKSKRDEGSSRNRNSELNDNLANASPQVPPPPQSEN 180
           SPIIEEEDKNENEGSVGGLRRRRSNKSKRDEGSSRNRNSELNDNLANASPQVPPPPQSEN
Sbjct: 121 SPIIEEEDKNENEGSVGGLRRRRSNKSKRDEGSSRNRNSELNDNLANASPQVPPPPQSEN 180

Query: 181 RHIPPPPQQNSTYDYFFSMDLPVSTFSEVDEVYINKEEIDIKPKVVDSDDIDEQRKSVKA 240
           RHIPPPPQQNSTYDYFFSMDLPVSTFSEVDEVYINKEEIDIKPKVVDSDDIDEQRKSVKA
Sbjct: 181 RHIPPPPQQNSTYDYFFSMDLPVSTFSEVDEVYINKEEIDIKPKVVDSDDIDEQRKSVKA 240

Query: 241 ETAEPLLEEPVEPPPSLPAEPATAVAKSSKKANQAGSMGSTEGKRMVKPDLNLLQIFIDI 300
           ETAEPLLEEPVEPPPSLPAEPATAVAKSSKKANQAGSMGSTEGKRMVKPDLNLLQIFIDI
Sbjct: 241 ETAEPLLEEPVEPPPSLPAEPATAVAKSSKKANQAGSMGSTEGKRMVKPDLNLLQIFIDI 300

Query: 301 DDHFLKSCESAREVSKMLEATRLHYHSNFADNRGHIDHSARVMRVITWNRSFRGLPNMDD 360
           DDHFLKSCESAREVSKMLEATRLHYHSNFADNRGHIDHSARVMRVITWNRSFRGLPNMDD
Sbjct: 301 DDHFLKSCESAREVSKMLEATRLHYHSNFADNRGHIDHSARVMRVITWNRSFRGLPNMDD 360

Query: 361 GKDDSYAEEQETHATVLDKLLAWEKKLYDEVKAGEVMKFEYQKKVATLNRLKKRDSNVEA 420
           GKDDSYAEEQETHATVLDKLLAWEKKLYDEVKAGEVMKFEYQKKVATLNRLKKRDSNVEA
Sbjct: 361 GKDDSYAEEQETHATVLDKLLAWEKKLYDEVKAGEVMKFEYQKKVATLNRLKKRDSNVEA 420

Query: 421 LEKAKAAVSHLHTRYIVDMQSLDSTVSEISRLRDEQLYPKLVQLVNGMATVWDTMRAHHE 480
           LEKAKAAVSHLHTRYIVDMQSLDSTVSEISRLRDEQLYPKLVQLVNGMATVWDTMRAHHE
Sbjct: 421 LEKAKAAVSHLHTRYIVDMQSLDSTVSEISRLRDEQLYPKLVQLVNGMATVWDTMRAHHE 480

Query: 481 MQLKIVSALRWMDLSQSPKETSTHHYERTVQLCGVVRDWHSQFEKLVRCQKDYIKALNSW 540
           MQLKIVSALRWMDLSQSPKETSTHHYERTVQLCGVVRDWHSQFEKLVRCQKDYIKALNSW
Sbjct: 481 MQLKIVSALRWMDLSQSPKETSTHHYERTVQLCGVVRDWHSQFEKLVRCQKDYIKALNSW 540

Query: 541 LKQNVVPIESSLKEKVSSPPRAQSPPIHKLLLAWDDQLERLPDEHLKTAIFTFGGVISTI 600
           LKQNVVPIESSLKEKVSSPPRAQSPPIHKLLLAWDDQLERLPDEHLKTAIFTFGGVISTI
Sbjct: 541 LKQNVVPIESSLKEKVSSPPRAQSPPIHKLLLAWDDQLERLPDEHLKTAIFTFGGVISTI 600

Query: 601 MLQQDDERKLMLKWEETEKELERKERHFNDWHYKYQQRRTPDELDPEKSEDNTQDSVVTE 660
           MLQQDDERKLMLKWEETEKELERKERHFNDWHYKYQQRRTPDELDPEKSEDNTQDSVVTE
Sbjct: 601 MLQQDDERKLMLKWEETEKELERKERHFNDWHYKYQQRRTPDELDPEKSEDNTQDSVVTE 660

Query: 661 KLIVVESLKRRLEEEKETHAKQCLHVREKSLLNLKNQLPELFRALSEFSFSSSEMYKNLR 720
           KLIVVESLKRRLEEEKETHAKQCLHVREKSLLNLKNQLPELFRALSEFSFSSSEMYKNLR
Sbjct: 661 KLIVVESLKRRLEEEKETHAKQCLHVREKSLLNLKNQLPELFRALSEFSFSSSEMYKNLR 720

Query: 721 SVCQV 726
           SVCQV
Sbjct: 721 SVCQV 725

BLAST of CmoCh01G001220 vs. ExPASy TrEMBL
Match: A0A6J1K8S0 (nitrate regulatory gene2 protein-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111493306 PE=4 SV=1)

HSP 1 Score: 1346.3 bits (3483), Expect = 0.0e+00
Identity = 694/725 (95.72%), Postives = 709/725 (97.79%), Query Frame = 0

Query: 1   MGCSQSKIDNEEAVARCKERKIHMKEAVTTRNAFAAAHSAYSMSLKNIGAVLSDYAHGEV 60
           MGCSQSKIDNEEA+ARCKERKIHMKEA T RNAFAAAHSAYSMSLKNIGA LSDYAHGEV
Sbjct: 1   MGCSQSKIDNEEAIARCKERKIHMKEAATARNAFAAAHSAYSMSLKNIGAALSDYAHGEV 60

Query: 61  ENPQFVYVSTQPNSAITSAPAPSEPFPPPPPLPPSNFPNSLERAATMPEMNVHKSDLKPE 120
           EN QFV VSTQPNSAITSAPAP EPFPPPPPLPPSNFPNSLERAATMPEMNVHKSDLKP 
Sbjct: 61  ENSQFVDVSTQPNSAITSAPAPIEPFPPPPPLPPSNFPNSLERAATMPEMNVHKSDLKPG 120

Query: 121 SPIIEEEDKNENEGSVGGLRRRRSNKSKRDEGSSRNRNSELNDNLANASPQVPPPPQSEN 180
           SPIIEEEDKNENEGSVGGLRRRRSNKSKRDEGSSRNRNSELNDNLA+ASPQVPPPP SEN
Sbjct: 121 SPIIEEEDKNENEGSVGGLRRRRSNKSKRDEGSSRNRNSELNDNLAHASPQVPPPPPSEN 180

Query: 181 RHIPPPPQQNSTYDYFFSMDLPVSTFSEVDEVYINKEEIDIKPKVVDSDDIDEQRKSVKA 240
           RHIPPPPQQNSTYDYFFSMDLPVSTFSEVDEVYIN+EEIDIKPKVVDSDDIDEQR+SVKA
Sbjct: 181 RHIPPPPQQNSTYDYFFSMDLPVSTFSEVDEVYINREEIDIKPKVVDSDDIDEQRRSVKA 240

Query: 241 ETAEPLLEEPVEPPPSLPAEPATAVAKSSKKANQAGSMGSTEGKRMVKPDLNLLQIFIDI 300
           ET EPLLEEPVEPPP LPAEPAT+V+KSSKK NQAGSMGSTEGKRMVKP+LNLLQIFIDI
Sbjct: 241 ETVEPLLEEPVEPPPCLPAEPATSVSKSSKKTNQAGSMGSTEGKRMVKPNLNLLQIFIDI 300

Query: 301 DDHFLKSCESAREVSKMLEATRLHYHSNFADNRGHIDHSARVMRVITWNRSFRGLPNMDD 360
           DDHFLKSCESAREVSKMLEATRLHYHSNFADNRGHIDHSARVMRVI WNRSFRGLPNMDD
Sbjct: 301 DDHFLKSCESAREVSKMLEATRLHYHSNFADNRGHIDHSARVMRVIAWNRSFRGLPNMDD 360

Query: 361 GKDDSYAEEQETHATVLDKLLAWEKKLYDEVKAGEVMKFEYQKKVATLNRLKKRDSNVEA 420
           GKDD Y EEQETHATVLDKLLAWEKKL DEVKAGE+MKFEYQKKVATLNRLKKRDSNVEA
Sbjct: 361 GKDDFYPEEQETHATVLDKLLAWEKKLSDEVKAGELMKFEYQKKVATLNRLKKRDSNVEA 420

Query: 421 LEKAKAAVSHLHTRYIVDMQSLDSTVSEISRLRDEQLYPKLVQLVNGMATVWDTMRAHHE 480
           LEKAKAAVSHLHTRYIVDMQSLDSTVSEISRLRDEQLYPKLVQLVNGMAT+WDTMRAHHE
Sbjct: 421 LEKAKAAVSHLHTRYIVDMQSLDSTVSEISRLRDEQLYPKLVQLVNGMATMWDTMRAHHE 480

Query: 481 MQLKIVSALRWMDLSQSPKETSTHHYERTVQLCGVVRDWHSQFEKLVRCQKDYIKALNSW 540
           MQLKIVSALR MDLSQSPKETSTHHYERTVQLCGVVRDWHSQFEKLVRCQKDYIKALNSW
Sbjct: 481 MQLKIVSALRSMDLSQSPKETSTHHYERTVQLCGVVRDWHSQFEKLVRCQKDYIKALNSW 540

Query: 541 LKQNVVPIESSLKEKVSSPPRAQSPPIHKLLLAWDDQLERLPDEHLKTAIFTFGGVISTI 600
           LKQN+VPIESSLKEKVSSPPRAQSPPIHKLLLAWDDQL+RLPDEHLKTAIFTFGGVI+TI
Sbjct: 541 LKQNLVPIESSLKEKVSSPPRAQSPPIHKLLLAWDDQLDRLPDEHLKTAIFTFGGVINTI 600

Query: 601 MLQQDDERKLMLKWEETEKELERKERHFNDWHYKYQQRRTPDELDPEKSEDNTQDSVVTE 660
           MLQQDDERKLMLKWEET+KELERKERHFNDWHYKYQQRRTPDELDPEKSEDN++DSVVTE
Sbjct: 601 MLQQDDERKLMLKWEETKKELERKERHFNDWHYKYQQRRTPDELDPEKSEDNSEDSVVTE 660

Query: 661 KLIVVESLKRRLEEEKETHAKQCLHVREKSLLNLKNQLPELFRALSEFSFSSSEMYKNLR 720
           KLIVVESLKRRLEEEKETHAKQCLHVREKSLLNLKNQLPELFRALSEFSFSSSEMYKNLR
Sbjct: 661 KLIVVESLKRRLEEEKETHAKQCLHVREKSLLNLKNQLPELFRALSEFSFSSSEMYKNLR 720

Query: 721 SVCQV 726
           SVCQV
Sbjct: 721 SVCQV 725

BLAST of CmoCh01G001220 vs. ExPASy TrEMBL
Match: A0A6J1G9J8 (nitrate regulatory gene2 protein-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111452183 PE=4 SV=1)

HSP 1 Score: 1202.2 bits (3109), Expect = 0.0e+00
Identity = 619/619 (100.00%), Postives = 619/619 (100.00%), Query Frame = 0

Query: 107 MPEMNVHKSDLKPESPIIEEEDKNENEGSVGGLRRRRSNKSKRDEGSSRNRNSELNDNLA 166
           MPEMNVHKSDLKPESPIIEEEDKNENEGSVGGLRRRRSNKSKRDEGSSRNRNSELNDNLA
Sbjct: 1   MPEMNVHKSDLKPESPIIEEEDKNENEGSVGGLRRRRSNKSKRDEGSSRNRNSELNDNLA 60

Query: 167 NASPQVPPPPQSENRHIPPPPQQNSTYDYFFSMDLPVSTFSEVDEVYINKEEIDIKPKVV 226
           NASPQVPPPPQSENRHIPPPPQQNSTYDYFFSMDLPVSTFSEVDEVYINKEEIDIKPKVV
Sbjct: 61  NASPQVPPPPQSENRHIPPPPQQNSTYDYFFSMDLPVSTFSEVDEVYINKEEIDIKPKVV 120

Query: 227 DSDDIDEQRKSVKAETAEPLLEEPVEPPPSLPAEPATAVAKSSKKANQAGSMGSTEGKRM 286
           DSDDIDEQRKSVKAETAEPLLEEPVEPPPSLPAEPATAVAKSSKKANQAGSMGSTEGKRM
Sbjct: 121 DSDDIDEQRKSVKAETAEPLLEEPVEPPPSLPAEPATAVAKSSKKANQAGSMGSTEGKRM 180

Query: 287 VKPDLNLLQIFIDIDDHFLKSCESAREVSKMLEATRLHYHSNFADNRGHIDHSARVMRVI 346
           VKPDLNLLQIFIDIDDHFLKSCESAREVSKMLEATRLHYHSNFADNRGHIDHSARVMRVI
Sbjct: 181 VKPDLNLLQIFIDIDDHFLKSCESAREVSKMLEATRLHYHSNFADNRGHIDHSARVMRVI 240

Query: 347 TWNRSFRGLPNMDDGKDDSYAEEQETHATVLDKLLAWEKKLYDEVKAGEVMKFEYQKKVA 406
           TWNRSFRGLPNMDDGKDDSYAEEQETHATVLDKLLAWEKKLYDEVKAGEVMKFEYQKKVA
Sbjct: 241 TWNRSFRGLPNMDDGKDDSYAEEQETHATVLDKLLAWEKKLYDEVKAGEVMKFEYQKKVA 300

Query: 407 TLNRLKKRDSNVEALEKAKAAVSHLHTRYIVDMQSLDSTVSEISRLRDEQLYPKLVQLVN 466
           TLNRLKKRDSNVEALEKAKAAVSHLHTRYIVDMQSLDSTVSEISRLRDEQLYPKLVQLVN
Sbjct: 301 TLNRLKKRDSNVEALEKAKAAVSHLHTRYIVDMQSLDSTVSEISRLRDEQLYPKLVQLVN 360

Query: 467 GMATVWDTMRAHHEMQLKIVSALRWMDLSQSPKETSTHHYERTVQLCGVVRDWHSQFEKL 526
           GMATVWDTMRAHHEMQLKIVSALRWMDLSQSPKETSTHHYERTVQLCGVVRDWHSQFEKL
Sbjct: 361 GMATVWDTMRAHHEMQLKIVSALRWMDLSQSPKETSTHHYERTVQLCGVVRDWHSQFEKL 420

Query: 527 VRCQKDYIKALNSWLKQNVVPIESSLKEKVSSPPRAQSPPIHKLLLAWDDQLERLPDEHL 586
           VRCQKDYIKALNSWLKQNVVPIESSLKEKVSSPPRAQSPPIHKLLLAWDDQLERLPDEHL
Sbjct: 421 VRCQKDYIKALNSWLKQNVVPIESSLKEKVSSPPRAQSPPIHKLLLAWDDQLERLPDEHL 480

Query: 587 KTAIFTFGGVISTIMLQQDDERKLMLKWEETEKELERKERHFNDWHYKYQQRRTPDELDP 646
           KTAIFTFGGVISTIMLQQDDERKLMLKWEETEKELERKERHFNDWHYKYQQRRTPDELDP
Sbjct: 481 KTAIFTFGGVISTIMLQQDDERKLMLKWEETEKELERKERHFNDWHYKYQQRRTPDELDP 540

Query: 647 EKSEDNTQDSVVTEKLIVVESLKRRLEEEKETHAKQCLHVREKSLLNLKNQLPELFRALS 706
           EKSEDNTQDSVVTEKLIVVESLKRRLEEEKETHAKQCLHVREKSLLNLKNQLPELFRALS
Sbjct: 541 EKSEDNTQDSVVTEKLIVVESLKRRLEEEKETHAKQCLHVREKSLLNLKNQLPELFRALS 600

Query: 707 EFSFSSSEMYKNLRSVCQV 726
           EFSFSSSEMYKNLRSVCQV
Sbjct: 601 EFSFSSSEMYKNLRSVCQV 619

BLAST of CmoCh01G001220 vs. ExPASy TrEMBL
Match: A0A6J1KHK4 (nitrate regulatory gene2 protein-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111493306 PE=4 SV=1)

HSP 1 Score: 1158.7 bits (2996), Expect = 0.0e+00
Identity = 595/619 (96.12%), Postives = 609/619 (98.38%), Query Frame = 0

Query: 107 MPEMNVHKSDLKPESPIIEEEDKNENEGSVGGLRRRRSNKSKRDEGSSRNRNSELNDNLA 166
           MPEMNVHKSDLKP SPIIEEEDKNENEGSVGGLRRRRSNKSKRDEGSSRNRNSELNDNLA
Sbjct: 1   MPEMNVHKSDLKPGSPIIEEEDKNENEGSVGGLRRRRSNKSKRDEGSSRNRNSELNDNLA 60

Query: 167 NASPQVPPPPQSENRHIPPPPQQNSTYDYFFSMDLPVSTFSEVDEVYINKEEIDIKPKVV 226
           +ASPQVPPPP SENRHIPPPPQQNSTYDYFFSMDLPVSTFSEVDEVYIN+EEIDIKPKVV
Sbjct: 61  HASPQVPPPPPSENRHIPPPPQQNSTYDYFFSMDLPVSTFSEVDEVYINREEIDIKPKVV 120

Query: 227 DSDDIDEQRKSVKAETAEPLLEEPVEPPPSLPAEPATAVAKSSKKANQAGSMGSTEGKRM 286
           DSDDIDEQR+SVKAET EPLLEEPVEPPP LPAEPAT+V+KSSKK NQAGSMGSTEGKRM
Sbjct: 121 DSDDIDEQRRSVKAETVEPLLEEPVEPPPCLPAEPATSVSKSSKKTNQAGSMGSTEGKRM 180

Query: 287 VKPDLNLLQIFIDIDDHFLKSCESAREVSKMLEATRLHYHSNFADNRGHIDHSARVMRVI 346
           VKP+LNLLQIFIDIDDHFLKSCESAREVSKMLEATRLHYHSNFADNRGHIDHSARVMRVI
Sbjct: 181 VKPNLNLLQIFIDIDDHFLKSCESAREVSKMLEATRLHYHSNFADNRGHIDHSARVMRVI 240

Query: 347 TWNRSFRGLPNMDDGKDDSYAEEQETHATVLDKLLAWEKKLYDEVKAGEVMKFEYQKKVA 406
            WNRSFRGLPNMDDGKDD Y EEQETHATVLDKLLAWEKKL DEVKAGE+MKFEYQKKVA
Sbjct: 241 AWNRSFRGLPNMDDGKDDFYPEEQETHATVLDKLLAWEKKLSDEVKAGELMKFEYQKKVA 300

Query: 407 TLNRLKKRDSNVEALEKAKAAVSHLHTRYIVDMQSLDSTVSEISRLRDEQLYPKLVQLVN 466
           TLNRLKKRDSNVEALEKAKAAVSHLHTRYIVDMQSLDSTVSEISRLRDEQLYPKLVQLVN
Sbjct: 301 TLNRLKKRDSNVEALEKAKAAVSHLHTRYIVDMQSLDSTVSEISRLRDEQLYPKLVQLVN 360

Query: 467 GMATVWDTMRAHHEMQLKIVSALRWMDLSQSPKETSTHHYERTVQLCGVVRDWHSQFEKL 526
           GMAT+WDTMRAHHEMQLKIVSALR MDLSQSPKETSTHHYERTVQLCGVVRDWHSQFEKL
Sbjct: 361 GMATMWDTMRAHHEMQLKIVSALRSMDLSQSPKETSTHHYERTVQLCGVVRDWHSQFEKL 420

Query: 527 VRCQKDYIKALNSWLKQNVVPIESSLKEKVSSPPRAQSPPIHKLLLAWDDQLERLPDEHL 586
           VRCQKDYIKALNSWLKQN+VPIESSLKEKVSSPPRAQSPPIHKLLLAWDDQL+RLPDEHL
Sbjct: 421 VRCQKDYIKALNSWLKQNLVPIESSLKEKVSSPPRAQSPPIHKLLLAWDDQLDRLPDEHL 480

Query: 587 KTAIFTFGGVISTIMLQQDDERKLMLKWEETEKELERKERHFNDWHYKYQQRRTPDELDP 646
           KTAIFTFGGVI+TIMLQQDDERKLMLKWEET+KELERKERHFNDWHYKYQQRRTPDELDP
Sbjct: 481 KTAIFTFGGVINTIMLQQDDERKLMLKWEETKKELERKERHFNDWHYKYQQRRTPDELDP 540

Query: 647 EKSEDNTQDSVVTEKLIVVESLKRRLEEEKETHAKQCLHVREKSLLNLKNQLPELFRALS 706
           EKSEDN++DSVVTEKLIVVESLKRRLEEEKETHAKQCLHVREKSLLNLKNQLPELFRALS
Sbjct: 541 EKSEDNSEDSVVTEKLIVVESLKRRLEEEKETHAKQCLHVREKSLLNLKNQLPELFRALS 600

Query: 707 EFSFSSSEMYKNLRSVCQV 726
           EFSFSSSEMYKNLRSVCQV
Sbjct: 601 EFSFSSSEMYKNLRSVCQV 619

BLAST of CmoCh01G001220 vs. ExPASy TrEMBL
Match: A0A6J1ISF0 (nitrate regulatory gene2 protein-like OS=Cucurbita maxima OX=3661 GN=LOC111480123 PE=4 SV=1)

HSP 1 Score: 1134.4 bits (2933), Expect = 0.0e+00
Identity = 603/735 (82.04%), Postives = 662/735 (90.07%), Query Frame = 0

Query: 1   MGCSQSKIDNEEAVARCKERKIHMKEAVTTRNAFAAAHSAYSMSLKNIGAVLSDYAHGEV 60
           MGCSQSKI+NEEA+ARCKERKIHMKEAVT RNAFAAAHSAYSMSLKN GA LSDYAHGEV
Sbjct: 1   MGCSQSKIENEEAIARCKERKIHMKEAVTFRNAFAAAHSAYSMSLKNTGAALSDYAHGEV 60

Query: 61  ENPQFVYVSTQPNSAITSAPAPS-EPF-PPPPPLPPSNFPNSLERAATMPEMNVHKSDLK 120
           +N QFV VSTQPNSA+TSA A S EPF PPPPPLPPSNF + L+RAATMPE+N++K DLK
Sbjct: 61  QNTQFVPVSTQPNSALTSATAASFEPFPPPPPPLPPSNFHSPLQRAATMPEINMYKPDLK 120

Query: 121 PESPIIEEEDKNENEGSVGGLRRRRSNKSKRDEGSSRNRNS-ELNDNLANASPQVPPPPQ 180
           P SPIIEEE++NENEGSVG LRRRRSNKSK DEGSSRNRNS ELN++LA ASP VPPPP 
Sbjct: 121 PGSPIIEEEEENENEGSVGALRRRRSNKSKGDEGSSRNRNSAELNEDLAGASPPVPPPP- 180

Query: 181 SENRHIPPPPQQNSTYDYFFSMD-LPVSTFSEVDEVYINKEEIDIK-----PKVVDSDDI 240
           SENRHIPPPPQQ+STYDYFFS+D +PVST SEV+EV INK EI+ K      K VD+ DI
Sbjct: 181 SENRHIPPPPQQDSTYDYFFSVDNIPVSTLSEVEEVQINKAEIERKSFDKMSKGVDNHDI 240

Query: 241 DEQRKSVKAETAEPLLEEPVEPPPSLPAEPATAV-AKSSKKANQAGSMGSTEGKRMVKPD 300
           +E+  S KAET E +LEEPV PPP+ P+   ++V AKS KK  Q GSMG+ +GKRMVKP+
Sbjct: 241 EERGISGKAETVESVLEEPVAPPPAPPSVAESSVAAKSLKKMKQGGSMGAMDGKRMVKPN 300

Query: 301 LNLLQIFIDIDDHFLKSCESAREVSKMLEATRLHYHSNFADNRGHIDHSARVMRVITWNR 360
           +NLL IF +IDD+FL++ ESA EVSKMLEATRLHYHSNFADNRGHIDHSARVMRVITWNR
Sbjct: 301 VNLLLIFTNIDDNFLQASESAHEVSKMLEATRLHYHSNFADNRGHIDHSARVMRVITWNR 360

Query: 361 SFRGLPNMDDGKDDSYAEEQETHATVLDKLLAWEKKLYDEVKAGEVMKFEYQKKVATLNR 420
           SFRGL NMDDGKDD YAEEQETHATVLDKLLAWEKKLYDEVKAGE+MKFEYQKKVATLNR
Sbjct: 361 SFRGLANMDDGKDDFYAEEQETHATVLDKLLAWEKKLYDEVKAGELMKFEYQKKVATLNR 420

Query: 421 LKKRDSNVEALEKAKAAVSHLHTRYIVDMQSLDSTVSEISRLRDEQLYPKLVQLVNGMAT 480
           LKKRDSN EALEKAKAAVSHLHTRYIVDMQSLDSTVSEISRLRDEQLYPKLVQLVNGM+ 
Sbjct: 421 LKKRDSNAEALEKAKAAVSHLHTRYIVDMQSLDSTVSEISRLRDEQLYPKLVQLVNGMSL 480

Query: 481 VWDTMRAHHEMQLKIVSALRWMDLSQSPKETSTHHYERTVQLCGVVRDWHSQFEKLVRCQ 540
           +WDTMRAHHE QLKIVSALR MDLSQSPKETSTHHYERTVQLCGVVR+WHSQFEKLVRCQ
Sbjct: 481 MWDTMRAHHETQLKIVSALRSMDLSQSPKETSTHHYERTVQLCGVVREWHSQFEKLVRCQ 540

Query: 541 KDYIKALNSWLKQNVVPIESSLKEKVSSPPRAQSPPIHKLLLAWDDQLERLPDEHLKTAI 600
           KDYI+ALNSWLK N++PIESSL+EKVSSPPR QSPPI KLL+AW DQLERLPDEHL+TAI
Sbjct: 541 KDYIRALNSWLKLNLIPIESSLREKVSSPPRVQSPPIQKLLIAWHDQLERLPDEHLRTAI 600

Query: 601 FTFGGVISTIMLQQDDERKLMLKWEETEKELERKERHFNDWHYKYQQRRTPDELDPEKSE 660
           FTFG VI+TIMLQQD+ERKL  KWEET KELERK+RHFN+WH KYQQRR PDELDPE+SE
Sbjct: 601 FTFGAVINTIMLQQDEERKLKTKWEETGKELERKQRHFNEWHNKYQQRRMPDELDPERSE 660

Query: 661 DNTQDSVVTEKLIVVESLKRRLEEEKETHAKQCLHVREKSLLNLKNQLPELFRALSEFSF 720
           +NTQD+ VT+KL+ VE LK+RLEEE ETHAKQCLHVREKSL++LKNQLP+LFRALSEFS 
Sbjct: 661 ENTQDAAVTDKLVAVELLKKRLEEEIETHAKQCLHVREKSLVSLKNQLPDLFRALSEFSL 720

Query: 721 SSSEMYKNLRSVCQV 726
           +SSEMYKNLRS+CQV
Sbjct: 721 ASSEMYKNLRSICQV 734

BLAST of CmoCh01G001220 vs. NCBI nr
Match: XP_022948532.1 (nitrate regulatory gene2 protein-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 1404.4 bits (3634), Expect = 0.0e+00
Identity = 725/725 (100.00%), Postives = 725/725 (100.00%), Query Frame = 0

Query: 1   MGCSQSKIDNEEAVARCKERKIHMKEAVTTRNAFAAAHSAYSMSLKNIGAVLSDYAHGEV 60
           MGCSQSKIDNEEAVARCKERKIHMKEAVTTRNAFAAAHSAYSMSLKNIGAVLSDYAHGEV
Sbjct: 1   MGCSQSKIDNEEAVARCKERKIHMKEAVTTRNAFAAAHSAYSMSLKNIGAVLSDYAHGEV 60

Query: 61  ENPQFVYVSTQPNSAITSAPAPSEPFPPPPPLPPSNFPNSLERAATMPEMNVHKSDLKPE 120
           ENPQFVYVSTQPNSAITSAPAPSEPFPPPPPLPPSNFPNSLERAATMPEMNVHKSDLKPE
Sbjct: 61  ENPQFVYVSTQPNSAITSAPAPSEPFPPPPPLPPSNFPNSLERAATMPEMNVHKSDLKPE 120

Query: 121 SPIIEEEDKNENEGSVGGLRRRRSNKSKRDEGSSRNRNSELNDNLANASPQVPPPPQSEN 180
           SPIIEEEDKNENEGSVGGLRRRRSNKSKRDEGSSRNRNSELNDNLANASPQVPPPPQSEN
Sbjct: 121 SPIIEEEDKNENEGSVGGLRRRRSNKSKRDEGSSRNRNSELNDNLANASPQVPPPPQSEN 180

Query: 181 RHIPPPPQQNSTYDYFFSMDLPVSTFSEVDEVYINKEEIDIKPKVVDSDDIDEQRKSVKA 240
           RHIPPPPQQNSTYDYFFSMDLPVSTFSEVDEVYINKEEIDIKPKVVDSDDIDEQRKSVKA
Sbjct: 181 RHIPPPPQQNSTYDYFFSMDLPVSTFSEVDEVYINKEEIDIKPKVVDSDDIDEQRKSVKA 240

Query: 241 ETAEPLLEEPVEPPPSLPAEPATAVAKSSKKANQAGSMGSTEGKRMVKPDLNLLQIFIDI 300
           ETAEPLLEEPVEPPPSLPAEPATAVAKSSKKANQAGSMGSTEGKRMVKPDLNLLQIFIDI
Sbjct: 241 ETAEPLLEEPVEPPPSLPAEPATAVAKSSKKANQAGSMGSTEGKRMVKPDLNLLQIFIDI 300

Query: 301 DDHFLKSCESAREVSKMLEATRLHYHSNFADNRGHIDHSARVMRVITWNRSFRGLPNMDD 360
           DDHFLKSCESAREVSKMLEATRLHYHSNFADNRGHIDHSARVMRVITWNRSFRGLPNMDD
Sbjct: 301 DDHFLKSCESAREVSKMLEATRLHYHSNFADNRGHIDHSARVMRVITWNRSFRGLPNMDD 360

Query: 361 GKDDSYAEEQETHATVLDKLLAWEKKLYDEVKAGEVMKFEYQKKVATLNRLKKRDSNVEA 420
           GKDDSYAEEQETHATVLDKLLAWEKKLYDEVKAGEVMKFEYQKKVATLNRLKKRDSNVEA
Sbjct: 361 GKDDSYAEEQETHATVLDKLLAWEKKLYDEVKAGEVMKFEYQKKVATLNRLKKRDSNVEA 420

Query: 421 LEKAKAAVSHLHTRYIVDMQSLDSTVSEISRLRDEQLYPKLVQLVNGMATVWDTMRAHHE 480
           LEKAKAAVSHLHTRYIVDMQSLDSTVSEISRLRDEQLYPKLVQLVNGMATVWDTMRAHHE
Sbjct: 421 LEKAKAAVSHLHTRYIVDMQSLDSTVSEISRLRDEQLYPKLVQLVNGMATVWDTMRAHHE 480

Query: 481 MQLKIVSALRWMDLSQSPKETSTHHYERTVQLCGVVRDWHSQFEKLVRCQKDYIKALNSW 540
           MQLKIVSALRWMDLSQSPKETSTHHYERTVQLCGVVRDWHSQFEKLVRCQKDYIKALNSW
Sbjct: 481 MQLKIVSALRWMDLSQSPKETSTHHYERTVQLCGVVRDWHSQFEKLVRCQKDYIKALNSW 540

Query: 541 LKQNVVPIESSLKEKVSSPPRAQSPPIHKLLLAWDDQLERLPDEHLKTAIFTFGGVISTI 600
           LKQNVVPIESSLKEKVSSPPRAQSPPIHKLLLAWDDQLERLPDEHLKTAIFTFGGVISTI
Sbjct: 541 LKQNVVPIESSLKEKVSSPPRAQSPPIHKLLLAWDDQLERLPDEHLKTAIFTFGGVISTI 600

Query: 601 MLQQDDERKLMLKWEETEKELERKERHFNDWHYKYQQRRTPDELDPEKSEDNTQDSVVTE 660
           MLQQDDERKLMLKWEETEKELERKERHFNDWHYKYQQRRTPDELDPEKSEDNTQDSVVTE
Sbjct: 601 MLQQDDERKLMLKWEETEKELERKERHFNDWHYKYQQRRTPDELDPEKSEDNTQDSVVTE 660

Query: 661 KLIVVESLKRRLEEEKETHAKQCLHVREKSLLNLKNQLPELFRALSEFSFSSSEMYKNLR 720
           KLIVVESLKRRLEEEKETHAKQCLHVREKSLLNLKNQLPELFRALSEFSFSSSEMYKNLR
Sbjct: 661 KLIVVESLKRRLEEEKETHAKQCLHVREKSLLNLKNQLPELFRALSEFSFSSSEMYKNLR 720

Query: 721 SVCQV 726
           SVCQV
Sbjct: 721 SVCQV 725

BLAST of CmoCh01G001220 vs. NCBI nr
Match: KAG7036484.1 (hypothetical protein SDJN02_00101, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1379.0 bits (3568), Expect = 0.0e+00
Identity = 711/725 (98.07%), Postives = 719/725 (99.17%), Query Frame = 0

Query: 1   MGCSQSKIDNEEAVARCKERKIHMKEAVTTRNAFAAAHSAYSMSLKNIGAVLSDYAHGEV 60
           MGCSQSKIDNEEA+ARCKERKIHMKEAVTTRNAFAAAHSAYSMSLKNIGA LSDYAHGEV
Sbjct: 1   MGCSQSKIDNEEAIARCKERKIHMKEAVTTRNAFAAAHSAYSMSLKNIGAALSDYAHGEV 60

Query: 61  ENPQFVYVSTQPNSAITSAPAPSEPFPPPPPLPPSNFPNSLERAATMPEMNVHKSDLKPE 120
           ENPQFV VSTQPNSAIT+APAPSEPFPPPPPLPPSNFPNSLERAATMPEMN HKSDLKP 
Sbjct: 61  ENPQFVDVSTQPNSAITTAPAPSEPFPPPPPLPPSNFPNSLERAATMPEMNGHKSDLKPG 120

Query: 121 SPIIEEEDKNENEGSVGGLRRRRSNKSKRDEGSSRNRNSELNDNLANASPQVPPPPQSEN 180
           SPIIE+ED+NENEGS G LRRRRSNKSKRDEGSSRNRNSELNDNLANASPQVPPPPQSEN
Sbjct: 121 SPIIEKEDENENEGSAGALRRRRSNKSKRDEGSSRNRNSELNDNLANASPQVPPPPQSEN 180

Query: 181 RHIPPPPQQNSTYDYFFSMDLPVSTFSEVDEVYINKEEIDIKPKVVDSDDIDEQRKSVKA 240
           RHIPPPPQQNSTYDYFFSMDLPVSTFSEVDEVYINKEEIDIKPKVVDSDDIDEQRKSVKA
Sbjct: 181 RHIPPPPQQNSTYDYFFSMDLPVSTFSEVDEVYINKEEIDIKPKVVDSDDIDEQRKSVKA 240

Query: 241 ETAEPLLEEPVEPPPSLPAEPATAVAKSSKKANQAGSMGSTEGKRMVKPDLNLLQIFIDI 300
           ETAEPLLEEPVEPPPSLPAEPATAVAKSSKKANQAGSMGSTEGKRMVKP+LNLLQIFIDI
Sbjct: 241 ETAEPLLEEPVEPPPSLPAEPATAVAKSSKKANQAGSMGSTEGKRMVKPNLNLLQIFIDI 300

Query: 301 DDHFLKSCESAREVSKMLEATRLHYHSNFADNRGHIDHSARVMRVITWNRSFRGLPNMDD 360
           DDHFLKSCESAREVSKMLEATRLHYHSNFADNRGHIDHSARVMRVITWNRSFRGLPNMDD
Sbjct: 301 DDHFLKSCESAREVSKMLEATRLHYHSNFADNRGHIDHSARVMRVITWNRSFRGLPNMDD 360

Query: 361 GKDDSYAEEQETHATVLDKLLAWEKKLYDEVKAGEVMKFEYQKKVATLNRLKKRDSNVEA 420
           GKDDSYAEEQETHATVLDKLLAWEKKLYDEVKAGEVMKFEYQKKVATLNRLKKRDSNVEA
Sbjct: 361 GKDDSYAEEQETHATVLDKLLAWEKKLYDEVKAGEVMKFEYQKKVATLNRLKKRDSNVEA 420

Query: 421 LEKAKAAVSHLHTRYIVDMQSLDSTVSEISRLRDEQLYPKLVQLVNGMATVWDTMRAHHE 480
           LEKAKAAVSHLHTRYIVDMQSLDSTVSEISRLRDEQLYPKLVQLVNGMAT+WDTMRAHHE
Sbjct: 421 LEKAKAAVSHLHTRYIVDMQSLDSTVSEISRLRDEQLYPKLVQLVNGMATMWDTMRAHHE 480

Query: 481 MQLKIVSALRWMDLSQSPKETSTHHYERTVQLCGVVRDWHSQFEKLVRCQKDYIKALNSW 540
           MQLKIVSALRWMDLSQSPKETSTHHYERTVQLCGVVRDWHSQFEKLVRCQKDYIKALNSW
Sbjct: 481 MQLKIVSALRWMDLSQSPKETSTHHYERTVQLCGVVRDWHSQFEKLVRCQKDYIKALNSW 540

Query: 541 LKQNVVPIESSLKEKVSSPPRAQSPPIHKLLLAWDDQLERLPDEHLKTAIFTFGGVISTI 600
           LKQNVVPIESSLKEKVSSPPRAQSPPIHKLLLAWDDQLERLPDEHLKTAIFTFGGVI+TI
Sbjct: 541 LKQNVVPIESSLKEKVSSPPRAQSPPIHKLLLAWDDQLERLPDEHLKTAIFTFGGVINTI 600

Query: 601 MLQQDDERKLMLKWEETEKELERKERHFNDWHYKYQQRRTPDELDPEKSEDNTQDSVVTE 660
           MLQQDDERKLMLKWEETEKELERKERHFNDWHYKYQQRRTPDELDPEKSEDNTQDSVVTE
Sbjct: 601 MLQQDDERKLMLKWEETEKELERKERHFNDWHYKYQQRRTPDELDPEKSEDNTQDSVVTE 660

Query: 661 KLIVVESLKRRLEEEKETHAKQCLHVREKSLLNLKNQLPELFRALSEFSFSSSEMYKNLR 720
           KLIVVESLKRRLEEEKETHAKQCLHVREKSLL+LKNQLPELFRALSEFSFSSSEMYKNLR
Sbjct: 661 KLIVVESLKRRLEEEKETHAKQCLHVREKSLLSLKNQLPELFRALSEFSFSSSEMYKNLR 720

Query: 721 SVCQV 726
           SVCQV
Sbjct: 721 SVCQV 725

BLAST of CmoCh01G001220 vs. NCBI nr
Match: KAG6606771.1 (Protein ROLLING AND ERECT LEAF 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1363.2 bits (3527), Expect = 0.0e+00
Identity = 705/721 (97.78%), Postives = 713/721 (98.89%), Query Frame = 0

Query: 1   MGCSQSKIDNEEAVARCKERKIHMKEAVTTRNAFAAAHSAYSMSLKNIGAVLSDYAHGEV 60
           MGCSQSKIDNEEA+ARCKERKIHMKEAVTTRNAFAAAHSAYSMSLKNIGA LSDYAHGEV
Sbjct: 1   MGCSQSKIDNEEAIARCKERKIHMKEAVTTRNAFAAAHSAYSMSLKNIGAALSDYAHGEV 60

Query: 61  ENPQFVYVSTQPNSAITSAPAPSEPFPPPPPLPPSNFPNSLERAATMPEMNVHKSDLKPE 120
           ENPQFV VSTQPNSAITSAPAPSEPFPPPPPLPP NFPNSLERAATMPEMN HKSDLKP 
Sbjct: 61  ENPQFVDVSTQPNSAITSAPAPSEPFPPPPPLPPPNFPNSLERAATMPEMNGHKSDLKPG 120

Query: 121 SPIIEEEDKNENEGSVGGLRRRRSNKSKRDEGSSRNRNSELNDNLANASPQVPPPPQSEN 180
           SPIIE+ED+NENEGS G LRRRRSNKSKRDEGSSRNRNSELNDNLANASPQVPPPPQSEN
Sbjct: 121 SPIIEKEDENENEGSAGALRRRRSNKSKRDEGSSRNRNSELNDNLANASPQVPPPPQSEN 180

Query: 181 RHIPPPPQQNSTYDYFFSMDLPVSTFSEVDEVYINKEEIDIKPKVVDSDDIDEQRKSVKA 240
           RHIPPPPQQNSTYDYFFSMDLPVSTFSEVDEVYINKEEIDIKPKVVDSDDIDEQRKSVKA
Sbjct: 181 RHIPPPPQQNSTYDYFFSMDLPVSTFSEVDEVYINKEEIDIKPKVVDSDDIDEQRKSVKA 240

Query: 241 ETAEPLLEEPVEPPPSLPAEPATAVAKSSKKANQAGSMGSTEGKRMVKPDLNLLQIFIDI 300
           ETAEPLLEEPVEPPP+LPAEPATAVAKSSKKANQAGSMGSTEGKRMVKP+LNLLQIFIDI
Sbjct: 241 ETAEPLLEEPVEPPPTLPAEPATAVAKSSKKANQAGSMGSTEGKRMVKPNLNLLQIFIDI 300

Query: 301 DDHFLKSCESAREVSKMLEATRLHYHSNFADNRGHIDHSARVMRVITWNRSFRGLPNMDD 360
           DDHFLKSCESAREVSKMLEATRLHYHSNFADNRGHIDHSARVMRVITWNRSFRGLPNMDD
Sbjct: 301 DDHFLKSCESAREVSKMLEATRLHYHSNFADNRGHIDHSARVMRVITWNRSFRGLPNMDD 360

Query: 361 GKDDSYAEEQETHATVLDKLLAWEKKLYDEVKAGEVMKFEYQKKVATLNRLKKRDSNVEA 420
           GKDDSYAEEQETHATVLDKLLAWEKKLYDEVKAGEVMKFEYQKKVATLNRLKKRDSNVEA
Sbjct: 361 GKDDSYAEEQETHATVLDKLLAWEKKLYDEVKAGEVMKFEYQKKVATLNRLKKRDSNVEA 420

Query: 421 LEKAKAAVSHLHTRYIVDMQSLDSTVSEISRLRDEQLYPKLVQLVNGMATVWDTMRAHHE 480
           LEKAKAAVSHLHTRYIVDMQSLDSTVSEISRLRDEQLYPKLVQLVNGMAT+WDTMRAHHE
Sbjct: 421 LEKAKAAVSHLHTRYIVDMQSLDSTVSEISRLRDEQLYPKLVQLVNGMATMWDTMRAHHE 480

Query: 481 MQLKIVSALRWMDLSQSPKETSTHHYERTVQLCGVVRDWHSQFEKLVRCQKDYIKALNSW 540
           MQLKIVSALR MDLSQSPKETSTHHYERTVQLCGVVRDWHSQFEKLVRCQKDYIKALNSW
Sbjct: 481 MQLKIVSALRSMDLSQSPKETSTHHYERTVQLCGVVRDWHSQFEKLVRCQKDYIKALNSW 540

Query: 541 LKQNVVPIESSLKEKVSSPPRAQSPPIHKLLLAWDDQLERLPDEHLKTAIFTFGGVISTI 600
           LKQNVVPIESSLKEKVSSPPRAQSPPIHKLLLAWDDQLERLPDEHLKTAIFTFGGVI+TI
Sbjct: 541 LKQNVVPIESSLKEKVSSPPRAQSPPIHKLLLAWDDQLERLPDEHLKTAIFTFGGVINTI 600

Query: 601 MLQQDDERKLMLKWEETEKELERKERHFNDWHYKYQQRRTPDELDPEKSEDNTQDSVVTE 660
           MLQQDDERKLMLKWEETEKELERKERHFNDWHYKYQQRRTPDELDPEKSEDNTQDSVVTE
Sbjct: 601 MLQQDDERKLMLKWEETEKELERKERHFNDWHYKYQQRRTPDELDPEKSEDNTQDSVVTE 660

Query: 661 KLIVVESLKRRLEEEKETHAKQCLHVREKSLLNLKNQLPELFRALSEFSFSSSEMYKNLR 720
           KLIVVESLKRRLEEEKETHAKQCLHVREKSLL+LKNQLPELFRALSEFSFSSSEMYKNLR
Sbjct: 661 KLIVVESLKRRLEEEKETHAKQCLHVREKSLLSLKNQLPELFRALSEFSFSSSEMYKNLR 720

Query: 721 S 722
           S
Sbjct: 721 S 721

BLAST of CmoCh01G001220 vs. NCBI nr
Match: XP_023524816.1 (nitrate regulatory gene2 protein-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1362.8 bits (3526), Expect = 0.0e+00
Identity = 706/725 (97.38%), Postives = 713/725 (98.34%), Query Frame = 0

Query: 1   MGCSQSKIDNEEAVARCKERKIHMKEAVTTRNAFAAAHSAYSMSLKNIGAVLSDYAHGEV 60
           MGCSQSKIDNEEA+ARCKERKIHMKEAVTTRNAFAAAHSAYSMSLKNIGA LSDYAHGEV
Sbjct: 1   MGCSQSKIDNEEAIARCKERKIHMKEAVTTRNAFAAAHSAYSMSLKNIGAALSDYAHGEV 60

Query: 61  ENPQFVYVSTQPNSAITSAPAPSEPFPPPPPLPPSNFPNSLERAATMPEMNVHKSDLKPE 120
           ENPQFV VSTQPNSAITSAPAP EPFPPPPPLPPSNFPNSLERAATMPEMNVHKSDLKP 
Sbjct: 61  ENPQFVDVSTQPNSAITSAPAPIEPFPPPPPLPPSNFPNSLERAATMPEMNVHKSDLKPG 120

Query: 121 SPIIEEEDKNENEGSVGGLRRRRSNKSKRDEGSSRNRNSELNDNLANASPQVPPPPQSEN 180
           SPIIEEEDKNENEGSVGGLRRRRSNKSKRDEGSSRNRNSELND LANASPQVPPPP SEN
Sbjct: 121 SPIIEEEDKNENEGSVGGLRRRRSNKSKRDEGSSRNRNSELNDILANASPQVPPPPPSEN 180

Query: 181 RHIPPPPQQNSTYDYFFSMDLPVSTFSEVDEVYINKEEIDIKPKVVDSDDIDEQRKSVKA 240
           RHIPPPPQQNSTYDYFFSMDLPVSTFSEVDEVYINKEEIDIKPKVVD DDIDE+R+SVKA
Sbjct: 181 RHIPPPPQQNSTYDYFFSMDLPVSTFSEVDEVYINKEEIDIKPKVVDIDDIDERRRSVKA 240

Query: 241 ETAEPLLEEPVEPPPSLPAEPATAVAKSSKKANQAGSMGSTEGKRMVKPDLNLLQIFIDI 300
           ETAEPLLEEPVEPPPSLPAEPATAVAKSSKK NQAGSMGS EGKRMVKP+LNLLQIFIDI
Sbjct: 241 ETAEPLLEEPVEPPPSLPAEPATAVAKSSKKTNQAGSMGSIEGKRMVKPNLNLLQIFIDI 300

Query: 301 DDHFLKSCESAREVSKMLEATRLHYHSNFADNRGHIDHSARVMRVITWNRSFRGLPNMDD 360
           DDHFLKSCESAREVSKMLEATRLHYHSNFADNRGHIDHSARVMRVITWNRSFRGLPNMDD
Sbjct: 301 DDHFLKSCESAREVSKMLEATRLHYHSNFADNRGHIDHSARVMRVITWNRSFRGLPNMDD 360

Query: 361 GKDDSYAEEQETHATVLDKLLAWEKKLYDEVKAGEVMKFEYQKKVATLNRLKKRDSNVEA 420
           GKDDSYAEEQETHATVLDKLLAWEKKLYDEVKAGEVMKFEYQKKVATLNRLKKRDSNVEA
Sbjct: 361 GKDDSYAEEQETHATVLDKLLAWEKKLYDEVKAGEVMKFEYQKKVATLNRLKKRDSNVEA 420

Query: 421 LEKAKAAVSHLHTRYIVDMQSLDSTVSEISRLRDEQLYPKLVQLVNGMATVWDTMRAHHE 480
           LEKAKAAVSHLHTRYIVDMQSLDSTVSEISRLRDEQLYPKLVQLVNGMAT+WDTMRAHHE
Sbjct: 421 LEKAKAAVSHLHTRYIVDMQSLDSTVSEISRLRDEQLYPKLVQLVNGMATMWDTMRAHHE 480

Query: 481 MQLKIVSALRWMDLSQSPKETSTHHYERTVQLCGVVRDWHSQFEKLVRCQKDYIKALNSW 540
           MQLKIVSALR MDLSQSPKETSTHHYERTVQLCGVVRDWHSQFEKLVRCQKDYIKALNSW
Sbjct: 481 MQLKIVSALRSMDLSQSPKETSTHHYERTVQLCGVVRDWHSQFEKLVRCQKDYIKALNSW 540

Query: 541 LKQNVVPIESSLKEKVSSPPRAQSPPIHKLLLAWDDQLERLPDEHLKTAIFTFGGVISTI 600
           LKQNVVPIESSLKEKVSSPPRAQSPPIHKLLLAWDDQLERLPDEHLKTAIFTFGGVI+TI
Sbjct: 541 LKQNVVPIESSLKEKVSSPPRAQSPPIHKLLLAWDDQLERLPDEHLKTAIFTFGGVINTI 600

Query: 601 MLQQDDERKLMLKWEETEKELERKERHFNDWHYKYQQRRTPDELDPEKSEDNTQDSVVTE 660
           MLQQDDERKLMLKWEETEKELERKERHFNDWHYKYQQRRTPDELDPEKSEDNTQDSVVTE
Sbjct: 601 MLQQDDERKLMLKWEETEKELERKERHFNDWHYKYQQRRTPDELDPEKSEDNTQDSVVTE 660

Query: 661 KLIVVESLKRRLEEEKETHAKQCLHVREKSLLNLKNQLPELFRALSEFSFSSSEMYKNLR 720
           KLI VES KRRLEEEKETHAKQCLHVREKSLL+LKNQLPELFRALSEFSFSSSEMYKNLR
Sbjct: 661 KLIEVESWKRRLEEEKETHAKQCLHVREKSLLSLKNQLPELFRALSEFSFSSSEMYKNLR 720

Query: 721 SVCQV 726
           SVCQV
Sbjct: 721 SVCQV 725

BLAST of CmoCh01G001220 vs. NCBI nr
Match: XP_022998727.1 (nitrate regulatory gene2 protein-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 1346.3 bits (3483), Expect = 0.0e+00
Identity = 694/725 (95.72%), Postives = 709/725 (97.79%), Query Frame = 0

Query: 1   MGCSQSKIDNEEAVARCKERKIHMKEAVTTRNAFAAAHSAYSMSLKNIGAVLSDYAHGEV 60
           MGCSQSKIDNEEA+ARCKERKIHMKEA T RNAFAAAHSAYSMSLKNIGA LSDYAHGEV
Sbjct: 1   MGCSQSKIDNEEAIARCKERKIHMKEAATARNAFAAAHSAYSMSLKNIGAALSDYAHGEV 60

Query: 61  ENPQFVYVSTQPNSAITSAPAPSEPFPPPPPLPPSNFPNSLERAATMPEMNVHKSDLKPE 120
           EN QFV VSTQPNSAITSAPAP EPFPPPPPLPPSNFPNSLERAATMPEMNVHKSDLKP 
Sbjct: 61  ENSQFVDVSTQPNSAITSAPAPIEPFPPPPPLPPSNFPNSLERAATMPEMNVHKSDLKPG 120

Query: 121 SPIIEEEDKNENEGSVGGLRRRRSNKSKRDEGSSRNRNSELNDNLANASPQVPPPPQSEN 180
           SPIIEEEDKNENEGSVGGLRRRRSNKSKRDEGSSRNRNSELNDNLA+ASPQVPPPP SEN
Sbjct: 121 SPIIEEEDKNENEGSVGGLRRRRSNKSKRDEGSSRNRNSELNDNLAHASPQVPPPPPSEN 180

Query: 181 RHIPPPPQQNSTYDYFFSMDLPVSTFSEVDEVYINKEEIDIKPKVVDSDDIDEQRKSVKA 240
           RHIPPPPQQNSTYDYFFSMDLPVSTFSEVDEVYIN+EEIDIKPKVVDSDDIDEQR+SVKA
Sbjct: 181 RHIPPPPQQNSTYDYFFSMDLPVSTFSEVDEVYINREEIDIKPKVVDSDDIDEQRRSVKA 240

Query: 241 ETAEPLLEEPVEPPPSLPAEPATAVAKSSKKANQAGSMGSTEGKRMVKPDLNLLQIFIDI 300
           ET EPLLEEPVEPPP LPAEPAT+V+KSSKK NQAGSMGSTEGKRMVKP+LNLLQIFIDI
Sbjct: 241 ETVEPLLEEPVEPPPCLPAEPATSVSKSSKKTNQAGSMGSTEGKRMVKPNLNLLQIFIDI 300

Query: 301 DDHFLKSCESAREVSKMLEATRLHYHSNFADNRGHIDHSARVMRVITWNRSFRGLPNMDD 360
           DDHFLKSCESAREVSKMLEATRLHYHSNFADNRGHIDHSARVMRVI WNRSFRGLPNMDD
Sbjct: 301 DDHFLKSCESAREVSKMLEATRLHYHSNFADNRGHIDHSARVMRVIAWNRSFRGLPNMDD 360

Query: 361 GKDDSYAEEQETHATVLDKLLAWEKKLYDEVKAGEVMKFEYQKKVATLNRLKKRDSNVEA 420
           GKDD Y EEQETHATVLDKLLAWEKKL DEVKAGE+MKFEYQKKVATLNRLKKRDSNVEA
Sbjct: 361 GKDDFYPEEQETHATVLDKLLAWEKKLSDEVKAGELMKFEYQKKVATLNRLKKRDSNVEA 420

Query: 421 LEKAKAAVSHLHTRYIVDMQSLDSTVSEISRLRDEQLYPKLVQLVNGMATVWDTMRAHHE 480
           LEKAKAAVSHLHTRYIVDMQSLDSTVSEISRLRDEQLYPKLVQLVNGMAT+WDTMRAHHE
Sbjct: 421 LEKAKAAVSHLHTRYIVDMQSLDSTVSEISRLRDEQLYPKLVQLVNGMATMWDTMRAHHE 480

Query: 481 MQLKIVSALRWMDLSQSPKETSTHHYERTVQLCGVVRDWHSQFEKLVRCQKDYIKALNSW 540
           MQLKIVSALR MDLSQSPKETSTHHYERTVQLCGVVRDWHSQFEKLVRCQKDYIKALNSW
Sbjct: 481 MQLKIVSALRSMDLSQSPKETSTHHYERTVQLCGVVRDWHSQFEKLVRCQKDYIKALNSW 540

Query: 541 LKQNVVPIESSLKEKVSSPPRAQSPPIHKLLLAWDDQLERLPDEHLKTAIFTFGGVISTI 600
           LKQN+VPIESSLKEKVSSPPRAQSPPIHKLLLAWDDQL+RLPDEHLKTAIFTFGGVI+TI
Sbjct: 541 LKQNLVPIESSLKEKVSSPPRAQSPPIHKLLLAWDDQLDRLPDEHLKTAIFTFGGVINTI 600

Query: 601 MLQQDDERKLMLKWEETEKELERKERHFNDWHYKYQQRRTPDELDPEKSEDNTQDSVVTE 660
           MLQQDDERKLMLKWEET+KELERKERHFNDWHYKYQQRRTPDELDPEKSEDN++DSVVTE
Sbjct: 601 MLQQDDERKLMLKWEETKKELERKERHFNDWHYKYQQRRTPDELDPEKSEDNSEDSVVTE 660

Query: 661 KLIVVESLKRRLEEEKETHAKQCLHVREKSLLNLKNQLPELFRALSEFSFSSSEMYKNLR 720
           KLIVVESLKRRLEEEKETHAKQCLHVREKSLLNLKNQLPELFRALSEFSFSSSEMYKNLR
Sbjct: 661 KLIVVESLKRRLEEEKETHAKQCLHVREKSLLNLKNQLPELFRALSEFSFSSSEMYKNLR 720

Query: 721 SVCQV 726
           SVCQV
Sbjct: 721 SVCQV 725

BLAST of CmoCh01G001220 vs. TAIR 10
Match: AT1G52320.2 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: N-terminal protein myristoylation; LOCATED IN: plasma membrane; EXPRESSED IN: 23 plant structures; EXPRESSED DURING: 14 growth stages; CONTAINS InterPro DOMAIN/s: Protein of unknown function DUF630 (InterPro:IPR006868), Protein of unknown function DUF632 (InterPro:IPR006867); BEST Arabidopsis thaliana protein match is: Protein of unknown function (DUF630 and DUF632) (TAIR:AT5G25590.1); Has 8725 Blast hits to 7476 proteins in 620 species: Archae - 10; Bacteria - 622; Metazoa - 3286; Fungi - 1319; Plants - 1442; Viruses - 221; Other Eukaryotes - 1825 (source: NCBI BLink). )

HSP 1 Score: 653.7 bits (1685), Expect = 1.8e-187
Identity = 396/778 (50.90%), Postives = 505/778 (64.91%), Query Frame = 0

Query: 1   MGCSQSKIDNEEAVARCKERKIHMKEAVTTRNAFAAAHSAYSMSLKNIGAVLSDYAHGEV 60
           MGC+QSKI+NEEAV RCKERK  MK+AVT RNAFAAAHSAY+M+LKN GA LSDY+HGE 
Sbjct: 1   MGCAQSKIENEEAVTRCKERKQLMKDAVTARNAFAAAHSAYAMALKNTGAALSDYSHGEF 60

Query: 61  -------ENPQFVYVSTQPNSAITSAPAPS------------------EPFP---PPPPL 120
                   +      ST       S P PS                  +P P   PPPP 
Sbjct: 61  LVSNHSSSSAAAAIASTSSLPTAISPPLPSSTAPVSNSTASSSSAAVPQPIPDTLPPPPP 120

Query: 121 PPSNFPNSLERAATMPEMNVHKSDLKPESPI--IEE--------EDKNENEGSVGGLRRR 180
           PP   P  L+RAATMPEMN         S +  IEE        +D ++++ S    R R
Sbjct: 121 PP---PLPLQRAATMPEMNGRSGGGHAGSGLNGIEEDGALDNDDDDDDDDDDSEMENRDR 180

Query: 181 RSNKSKRDEGSSRNRNSELNDNLANASPQVPPPPQSENRHIPPP---------PQQNSTY 240
              KS+   GS+R   + + D+        PPPP + +R IPPP          QQ   Y
Sbjct: 181 LIRKSRSRGGSTRGNRTTIEDHHLQEEKAPPPPPLANSRPIPPPRQHQHQHQQQQQQPFY 240

Query: 241 DYFFS--MDLPVSTFSEVDEVYINKEEIDIKPK----VVDSDDIDEQRKSVKAETAE--- 300
           DYFF    ++P +T  +       +    + P+    VV  DD DE+ +  + E  E   
Sbjct: 241 DYFFPNVENMPGTTLEDTPPQPQPQPTRPVPPQPHSPVVTEDDEDEEEEEEEEEEEEETV 300

Query: 301 ----PLLEEPVEPPPSLPAE-PATAVAKSSKKANQAGSMGSTEGKRMVKPDLNLLQIFID 360
               PL+EE  +    +  E       +  KK+   G  G   G RM     +L  +FI+
Sbjct: 301 IERKPLVEERPKRVEEVTIELEKVTNLRGMKKSKGIGIPGERRGMRMPVTATHLANVFIE 360

Query: 361 IDDHFLKSCESAREVSKMLEATRLHYHSNFADNRGHIDHSARVMRVITWNRSFRGLPNMD 420
           +DD+FLK+ ESA +VSKMLEATRLHYHSNFADNRGHIDHSARVMRVITWNRSFRG+PN D
Sbjct: 361 LDDNFLKASESAHDVSKMLEATRLHYHSNFADNRGHIDHSARVMRVITWNRSFRGIPNAD 420

Query: 421 DGKDDSYAEEQETHATVLDKLLAWEKKLYDEVKAGEVMKFEYQKKVATLNRLKKRDSNVE 480
           DGKDD   EE ETHATVLDKLLAWEKKLYDEVKAGE+MK EYQKKVA LNR+KKR  + +
Sbjct: 421 DGKDDVDLEENETHATVLDKLLAWEKKLYDEVKAGELMKIEYQKKVAHLNRVKKRGGHSD 480

Query: 481 ALEKAKAAVSHLHTRYIVDMQSLDSTVSEISRLRDEQLYPKLVQLVNGMATVWDTMRAHH 540
           +LE+AKAAVSHLHTRYIVDMQS+DSTVSEI+RLRDEQLY KLV LV  M  +W+ M+ HH
Sbjct: 481 SLERAKAAVSHLHTRYIVDMQSMDSTVSEINRLRDEQLYLKLVHLVEAMGKMWEMMQIHH 540

Query: 541 EMQLKIVSALRWMDLSQSPKETSTHHYERTVQLCGVVRDWHSQFEKLVRCQKDYIKALNS 600
           + Q +I   LR +D+SQ+ KET+ HH+ERT+QL  VV++WH+QF +++  QK+YIKAL  
Sbjct: 541 QRQAEISKVLRSLDVSQAVKETNDHHHERTIQLLAVVQEWHTQFCRMIDHQKEYIKALGG 600

Query: 601 WLKQNVVPIESSLKEKVSSPPRAQSPPIHKLLLAWDDQLERLPDEHLKTAIFTFGGVIST 660
           WLK N++PIES+LKEKVSSPPR  +P I KLL AW D+L+++PDE  K+AI  F  V+ST
Sbjct: 601 WLKLNLIPIESTLKEKVSSPPRVPNPAIQKLLHAWYDRLDKIPDEMAKSAIINFAAVVST 660

Query: 661 IMLQQDDERKLMLKWEETEKELERKERHFNDWHYKYQQRRTPDELDPEKSEDNTQDSVVT 718
           IM QQ+DE  L  K EET KEL RK R F DW++KY Q+R P+ ++P++++++  D V  
Sbjct: 661 IMQQQEDEISLRNKCEETRKELGRKIRQFEDWYHKYIQKRGPEGMNPDEADNDHNDEVAV 720

BLAST of CmoCh01G001220 vs. TAIR 10
Match: AT5G25590.1 (Protein of unknown function (DUF630 and DUF632) )

HSP 1 Score: 590.5 bits (1521), Expect = 1.8e-168
Identity = 366/779 (46.98%), Postives = 485/779 (62.26%), Query Frame = 0

Query: 1   MGCSQSKIDNEEAVARCKERKIHMKEAVTTRNAFAAAHSAYSMSLKNIGAVLSDYAHGEV 60
           MGC+QS++DNEEAVARCKER+  +KEAV+   AFAA H AY+++LKN GA LSDY HGE 
Sbjct: 1   MGCAQSRVDNEEAVARCKERRNVIKEAVSASKAFAAGHFAYAIALKNTGAALSDYGHGES 60

Query: 61  ENPQFVYV-------------STQPNSAITSAPAPSEPFPPPPPLPPSNFPNSLERAATM 120
           +      V             +  P S     P P E  PPPPP  P   P+ ++RA ++
Sbjct: 61  DQKALDDVLLDQQHYEKQSRNNVDPASPQPPPPPPIENLPPPPPPLPKFSPSPIKRAISL 120

Query: 121 PEMNV--HKSDLKPESPIIEEEDKNENEGSVGGLRRRRSNKSKR-------DEGSSRNRN 180
           P M V   K        I EEE+  E E  V G  R  + + +         + + R R 
Sbjct: 121 PSMAVRGRKVQTLDGMAIEEEEEDEEEEEEVKGSGRDTAQEEEEPRTPENVGKSNGRKRL 180

Query: 181 SELNDNLANASP------------QVPPPPQSENRHIPPPPQQNSTYDYFFSMDLPVSTF 240
            +    + +ASP            +  P P  ++R +    +  S++  F   D      
Sbjct: 181 EKTTPEIVSASPANSMAWDYFFMVENMPGPNLDDREVRNGYENQSSHFQFNEEDDEEEEE 240

Query: 241 SEVDEVYINK-------EEIDIK--PKVVDSDDIDEQRKSVKAETAEPLL---------- 300
            E   +Y  K       EE++ K   KV + ++ DE+    + E  E  +          
Sbjct: 241 EERSGIYRKKSGSGKVVEEMEPKTPEKVEEEEEEDEEEDEEEEEEEEEEVVVEVKKKKKG 300

Query: 301 EEPVEPPPSLPAEPATAVAKSSKKANQAGSMGSTEGKRMVKPDLNLLQIFIDIDDHFLKS 360
           +  +E   + P E   AVAK+S  A+ +               +NL++I  +IDD FLK+
Sbjct: 301 KAKIEHSSTAPPEFRRAVAKTSAAASSS---------------VNLMKILDEIDDRFLKA 360

Query: 361 CESAREVSKMLEATRLHYHSNFADNRGHIDHSARVMRVITWNRSFRGLPNMDDGKDDSYA 420
            E A+EVSKMLEATRLHYHSNFADNRG++DHSARVMRVITWN+S RG+ N + GKDD  +
Sbjct: 361 SECAQEVSKMLEATRLHYHSNFADNRGYVDHSARVMRVITWNKSLRGISNGEGGKDDQES 420

Query: 421 EEQETHATVLDKLLAWEKKLYDEVKAGEVMKFEYQKKVATLNRLKKRDSNVEALEKAKAA 480
           +E ETHATVLDKLLAWEKKLYDEVK GE+MK EYQKKV+ LNR KKR ++ E +EK KAA
Sbjct: 421 DEHETHATVLDKLLAWEKKLYDEVKQGELMKIEYQKKVSLLNRHKKRGASAETVEKTKAA 480

Query: 481 VSHLHTRYIVDMQSLDSTVSEISRLRDEQLYPKLVQLVNGMATVWDTMRAHHEMQLKIVS 540
           VSHLHTRYIVDMQS+DSTVSE++RLRD+QLYP+LV LV GMA +W  M  HH+ QL IV 
Sbjct: 481 VSHLHTRYIVDMQSMDSTVSEVNRLRDDQLYPRLVALVEGMAKMWTNMCIHHDTQLGIVG 540

Query: 541 ALRWMDLSQSPKETSTHHYERTVQLCGVVRDWHSQFEKLVRCQKDYIKALNSWLKQNVVP 600
            L+ +++S S KET+  H+ +T Q C V+ +WH QF+ LV  QK YI +LN+WLK N++P
Sbjct: 541 ELKALEISTSLKETTKQHHHQTRQFCTVLEEWHVQFDTLVTHQKQYINSLNNWLKLNLIP 600

Query: 601 IESSLKEKVSSPPRAQSPPIHKLLLAWDDQLERLPDEHLKTAIFTFGGVISTIMLQQDDE 660
           IESSLKEKVSSPPR Q PPI  LL +W D+LE+LPDE  K+AI +F  VI TI+L Q++E
Sbjct: 601 IESSLKEKVSSPPRPQRPPIQALLHSWHDRLEKLPDEVAKSAISSFAAVIKTILLHQEEE 660

Query: 661 RKLMLKWEETEKELERKERHFNDWHYKYQQRRTPDELDPEKSEDNTQDS--VVTEKLIVV 720
            KL  K EET +E  RK++ F DW+ K+ Q+R P E + E  +D T  S   VTE+ I V
Sbjct: 661 MKLKEKCEETRREFIRKKQGFEDWYQKHLQKRGPTE-EAEGGDDATTSSRDHVTERRIAV 720

Query: 721 ESLKRRLEEEKETHAKQCLHVREKSLLNLKNQLPELFRALSEFSFSSSEMYKNLRSVCQ 725
           E+LK+RLEEE+E H + C+ VREKSL +LK +LPE+FRALS+++ + ++ Y+ LR + Q
Sbjct: 721 ETLKKRLEEEEEAHQRHCVQVREKSLNSLKIRLPEIFRALSDYAHACADSYEKLRIISQ 763

BLAST of CmoCh01G001220 vs. TAIR 10
Match: AT1G52320.1 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: N-terminal protein myristoylation; LOCATED IN: plasma membrane; EXPRESSED IN: 23 plant structures; EXPRESSED DURING: 14 growth stages; CONTAINS InterPro DOMAIN/s: Protein of unknown function DUF632 (InterPro:IPR006867); BEST Arabidopsis thaliana protein match is: Protein of unknown function (DUF630 and DUF632) (TAIR:AT5G25590.1); Has 517 Blast hits to 513 proteins in 62 species: Archae - 6; Bacteria - 6; Metazoa - 50; Fungi - 2; Plants - 427; Viruses - 0; Other Eukaryotes - 26 (source: NCBI BLink). )

HSP 1 Score: 548.5 bits (1412), Expect = 8.0e-156
Identity = 278/448 (62.05%), Postives = 351/448 (78.35%), Query Frame = 0

Query: 270 KKANQAGSMGSTEGKRMVKPDLNLLQIFIDIDDHFLKSCESAREVSKMLEATRLHYHSNF 329
           KK+   G  G   G RM     +L  +FI++DD+FLK+ ESA +VSKMLEATRLHYHSNF
Sbjct: 2   KKSKGIGIPGERRGMRMPVTATHLANVFIELDDNFLKASESAHDVSKMLEATRLHYHSNF 61

Query: 330 ADNRGHIDHSARVMRVITWNRSFRGLPNMDDGKDDSYAEEQETHATVLDKLLAWEKKLYD 389
           ADNRGHIDHSARVMRVITWNRSFRG+PN DDGKDD   EE ETHATVLDKLLAWEKKLYD
Sbjct: 62  ADNRGHIDHSARVMRVITWNRSFRGIPNADDGKDDVDLEENETHATVLDKLLAWEKKLYD 121

Query: 390 EVKAGEVMKFEYQKKVATLNRLKKRDSNVEALEKAKAAVSHLHTRYIVDMQSLDSTVSEI 449
           EVKAGE+MK EYQKKVA LNR+KKR  + ++LE+AKAAVSHLHTRYIVDMQS+DSTVSEI
Sbjct: 122 EVKAGELMKIEYQKKVAHLNRVKKRGGHSDSLERAKAAVSHLHTRYIVDMQSMDSTVSEI 181

Query: 450 SRLRDEQLYPKLVQLVNGMATVWDTMRAHHEMQLKIVSALRWMDLSQSPKETSTHHYERT 509
           +RLRDEQLY KLV LV  M  +W+ M+ HH+ Q +I   LR +D+SQ+ KET+ HH+ERT
Sbjct: 182 NRLRDEQLYLKLVHLVEAMGKMWEMMQIHHQRQAEISKVLRSLDVSQAVKETNDHHHERT 241

Query: 510 VQLCGVVRDWHSQFEKLVRCQKDYIKALNSWLKQNVVPIESSLKEKVSSPPRAQSPPIHK 569
           +QL  VV++WH+QF +++  QK+YIKAL  WLK N++PIES+LKEKVSSPPR  +P I K
Sbjct: 242 IQLLAVVQEWHTQFCRMIDHQKEYIKALGGWLKLNLIPIESTLKEKVSSPPRVPNPAIQK 301

Query: 570 LLLAWDDQLERLPDEHLKTAIFTFGGVISTIMLQQDDERKLMLKWEETEKELERKERHFN 629
           LL AW D+L+++PDE  K+AI  F  V+STIM QQ+DE  L  K EET KEL RK R F 
Sbjct: 302 LLHAWYDRLDKIPDEMAKSAIINFAAVVSTIMQQQEDEISLRNKCEETRKELGRKIRQFE 361

Query: 630 DWHYKYQQRRTPDELDPEKSEDNTQDSVVTEKLIVVESLKRRLEEEKETHAKQCLHVREK 689
           DW++KY Q+R P+ ++P++++++  D V   +   VE +K+RLEEE+E + +Q   VREK
Sbjct: 362 DWYHKYIQKRGPEGMNPDEADNDHNDEVAVRQ-FNVEQIKKRLEEEEEAYHRQSHQVREK 421

Query: 690 SLLNLKNQLPELFRALSEFSFSSSEMYK 718
           SL +L+ +LPELF+A+SE ++S S+MY+
Sbjct: 422 SLASLRTRLPELFQAMSEVAYSCSDMYR 448

BLAST of CmoCh01G001220 vs. TAIR 10
Match: AT1G52320.3 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: N-terminal protein myristoylation; LOCATED IN: plasma membrane; EXPRESSED IN: 23 plant structures; EXPRESSED DURING: 14 growth stages; CONTAINS InterPro DOMAIN/s: Protein of unknown function DUF632 (InterPro:IPR006867); BEST Arabidopsis thaliana protein match is: Protein of unknown function (DUF630 and DUF632) (TAIR:AT5G25590.1); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink). )

HSP 1 Score: 548.5 bits (1412), Expect = 8.0e-156
Identity = 278/448 (62.05%), Postives = 351/448 (78.35%), Query Frame = 0

Query: 270 KKANQAGSMGSTEGKRMVKPDLNLLQIFIDIDDHFLKSCESAREVSKMLEATRLHYHSNF 329
           KK+   G  G   G RM     +L  +FI++DD+FLK+ ESA +VSKMLEATRLHYHSNF
Sbjct: 2   KKSKGIGIPGERRGMRMPVTATHLANVFIELDDNFLKASESAHDVSKMLEATRLHYHSNF 61

Query: 330 ADNRGHIDHSARVMRVITWNRSFRGLPNMDDGKDDSYAEEQETHATVLDKLLAWEKKLYD 389
           ADNRGHIDHSARVMRVITWNRSFRG+PN DDGKDD   EE ETHATVLDKLLAWEKKLYD
Sbjct: 62  ADNRGHIDHSARVMRVITWNRSFRGIPNADDGKDDVDLEENETHATVLDKLLAWEKKLYD 121

Query: 390 EVKAGEVMKFEYQKKVATLNRLKKRDSNVEALEKAKAAVSHLHTRYIVDMQSLDSTVSEI 449
           EVKAGE+MK EYQKKVA LNR+KKR  + ++LE+AKAAVSHLHTRYIVDMQS+DSTVSEI
Sbjct: 122 EVKAGELMKIEYQKKVAHLNRVKKRGGHSDSLERAKAAVSHLHTRYIVDMQSMDSTVSEI 181

Query: 450 SRLRDEQLYPKLVQLVNGMATVWDTMRAHHEMQLKIVSALRWMDLSQSPKETSTHHYERT 509
           +RLRDEQLY KLV LV  M  +W+ M+ HH+ Q +I   LR +D+SQ+ KET+ HH+ERT
Sbjct: 182 NRLRDEQLYLKLVHLVEAMGKMWEMMQIHHQRQAEISKVLRSLDVSQAVKETNDHHHERT 241

Query: 510 VQLCGVVRDWHSQFEKLVRCQKDYIKALNSWLKQNVVPIESSLKEKVSSPPRAQSPPIHK 569
           +QL  VV++WH+QF +++  QK+YIKAL  WLK N++PIES+LKEKVSSPPR  +P I K
Sbjct: 242 IQLLAVVQEWHTQFCRMIDHQKEYIKALGGWLKLNLIPIESTLKEKVSSPPRVPNPAIQK 301

Query: 570 LLLAWDDQLERLPDEHLKTAIFTFGGVISTIMLQQDDERKLMLKWEETEKELERKERHFN 629
           LL AW D+L+++PDE  K+AI  F  V+STIM QQ+DE  L  K EET KEL RK R F 
Sbjct: 302 LLHAWYDRLDKIPDEMAKSAIINFAAVVSTIMQQQEDEISLRNKCEETRKELGRKIRQFE 361

Query: 630 DWHYKYQQRRTPDELDPEKSEDNTQDSVVTEKLIVVESLKRRLEEEKETHAKQCLHVREK 689
           DW++KY Q+R P+ ++P++++++  D V   +   VE +K+RLEEE+E + +Q   VREK
Sbjct: 362 DWYHKYIQKRGPEGMNPDEADNDHNDEVAVRQ-FNVEQIKKRLEEEEEAYHRQSHQVREK 421

Query: 690 SLLNLKNQLPELFRALSEFSFSSSEMYK 718
           SL +L+ +LPELF+A+SE ++S S+MY+
Sbjct: 422 SLASLRTRLPELFQAMSEVAYSCSDMYR 448

BLAST of CmoCh01G001220 vs. TAIR 10
Match: AT1G52320.4 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: N-terminal protein myristoylation; LOCATED IN: plasma membrane; EXPRESSED IN: 23 plant structures; EXPRESSED DURING: 14 growth stages; CONTAINS InterPro DOMAIN/s: Protein of unknown function DUF632 (InterPro:IPR006867); BEST Arabidopsis thaliana protein match is: Protein of unknown function (DUF630 and DUF632) (TAIR:AT5G25590.1); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink). )

HSP 1 Score: 548.5 bits (1412), Expect = 8.0e-156
Identity = 278/448 (62.05%), Postives = 351/448 (78.35%), Query Frame = 0

Query: 270 KKANQAGSMGSTEGKRMVKPDLNLLQIFIDIDDHFLKSCESAREVSKMLEATRLHYHSNF 329
           KK+   G  G   G RM     +L  +FI++DD+FLK+ ESA +VSKMLEATRLHYHSNF
Sbjct: 2   KKSKGIGIPGERRGMRMPVTATHLANVFIELDDNFLKASESAHDVSKMLEATRLHYHSNF 61

Query: 330 ADNRGHIDHSARVMRVITWNRSFRGLPNMDDGKDDSYAEEQETHATVLDKLLAWEKKLYD 389
           ADNRGHIDHSARVMRVITWNRSFRG+PN DDGKDD   EE ETHATVLDKLLAWEKKLYD
Sbjct: 62  ADNRGHIDHSARVMRVITWNRSFRGIPNADDGKDDVDLEENETHATVLDKLLAWEKKLYD 121

Query: 390 EVKAGEVMKFEYQKKVATLNRLKKRDSNVEALEKAKAAVSHLHTRYIVDMQSLDSTVSEI 449
           EVKAGE+MK EYQKKVA LNR+KKR  + ++LE+AKAAVSHLHTRYIVDMQS+DSTVSEI
Sbjct: 122 EVKAGELMKIEYQKKVAHLNRVKKRGGHSDSLERAKAAVSHLHTRYIVDMQSMDSTVSEI 181

Query: 450 SRLRDEQLYPKLVQLVNGMATVWDTMRAHHEMQLKIVSALRWMDLSQSPKETSTHHYERT 509
           +RLRDEQLY KLV LV  M  +W+ M+ HH+ Q +I   LR +D+SQ+ KET+ HH+ERT
Sbjct: 182 NRLRDEQLYLKLVHLVEAMGKMWEMMQIHHQRQAEISKVLRSLDVSQAVKETNDHHHERT 241

Query: 510 VQLCGVVRDWHSQFEKLVRCQKDYIKALNSWLKQNVVPIESSLKEKVSSPPRAQSPPIHK 569
           +QL  VV++WH+QF +++  QK+YIKAL  WLK N++PIES+LKEKVSSPPR  +P I K
Sbjct: 242 IQLLAVVQEWHTQFCRMIDHQKEYIKALGGWLKLNLIPIESTLKEKVSSPPRVPNPAIQK 301

Query: 570 LLLAWDDQLERLPDEHLKTAIFTFGGVISTIMLQQDDERKLMLKWEETEKELERKERHFN 629
           LL AW D+L+++PDE  K+AI  F  V+STIM QQ+DE  L  K EET KEL RK R F 
Sbjct: 302 LLHAWYDRLDKIPDEMAKSAIINFAAVVSTIMQQQEDEISLRNKCEETRKELGRKIRQFE 361

Query: 630 DWHYKYQQRRTPDELDPEKSEDNTQDSVVTEKLIVVESLKRRLEEEKETHAKQCLHVREK 689
           DW++KY Q+R P+ ++P++++++  D V   +   VE +K+RLEEE+E + +Q   VREK
Sbjct: 362 DWYHKYIQKRGPEGMNPDEADNDHNDEVAVRQ-FNVEQIKKRLEEEEEAYHRQSHQVREK 421

Query: 690 SLLNLKNQLPELFRALSEFSFSSSEMYK 718
           SL +L+ +LPELF+A+SE ++S S+MY+
Sbjct: 422 SLASLRTRLPELFQAMSEVAYSCSDMYR 448

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9AQW11.7e-5729.03Protein ROLLING AND ERECT LEAF 2 OS=Oryza sativa subsp. japonica OX=39947 GN=REL... [more]
A0A178VBJ07.8e-5526.37Protein ALTERED PHOSPHATE STARVATION RESPONSE 1 OS=Arabidopsis thaliana OX=3702 ... [more]
Q93YU84.0e-5127.65Nitrate regulatory gene2 protein OS=Arabidopsis thaliana OX=3702 GN=NRG2 PE=1 SV... [more]
Match NameE-valueIdentityDescription
A0A6J1G9I10.0e+00100.00nitrate regulatory gene2 protein-like isoform X1 OS=Cucurbita moschata OX=3662 G... [more]
A0A6J1K8S00.0e+0095.72nitrate regulatory gene2 protein-like isoform X1 OS=Cucurbita maxima OX=3661 GN=... [more]
A0A6J1G9J80.0e+00100.00nitrate regulatory gene2 protein-like isoform X2 OS=Cucurbita moschata OX=3662 G... [more]
A0A6J1KHK40.0e+0096.12nitrate regulatory gene2 protein-like isoform X2 OS=Cucurbita maxima OX=3661 GN=... [more]
A0A6J1ISF00.0e+0082.04nitrate regulatory gene2 protein-like OS=Cucurbita maxima OX=3661 GN=LOC11148012... [more]
Match NameE-valueIdentityDescription
XP_022948532.10.0e+00100.00nitrate regulatory gene2 protein-like isoform X1 [Cucurbita moschata][more]
KAG7036484.10.0e+0098.07hypothetical protein SDJN02_00101, partial [Cucurbita argyrosperma subsp. argyro... [more]
KAG6606771.10.0e+0097.78Protein ROLLING AND ERECT LEAF 2, partial [Cucurbita argyrosperma subsp. sororia... [more]
XP_023524816.10.0e+0097.38nitrate regulatory gene2 protein-like isoform X1 [Cucurbita pepo subsp. pepo][more]
XP_022998727.10.0e+0095.72nitrate regulatory gene2 protein-like isoform X1 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
AT1G52320.21.8e-18750.90unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: N-termin... [more]
AT5G25590.11.8e-16846.98Protein of unknown function (DUF630 and DUF632) [more]
AT1G52320.18.0e-15662.05unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: N-termin... [more]
AT1G52320.38.0e-15662.05unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: N-termin... [more]
AT1G52320.48.0e-15662.05unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: N-termin... [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 669..689
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 225..246
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 169..183
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 225..284
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 81..97
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 113..157
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 75..194
NoneNo IPR availablePANTHERPTHR21450:SF33PROTEIN, PUTATIVE, 48652-45869-RELATEDcoord: 1..724
NoneNo IPR availablePANTHERPTHR21450UNCHARACTERIZEDcoord: 1..724
IPR006868Domain of unknown function DUF630PFAMPF04783DUF630coord: 1..59
e-value: 8.2E-24
score: 83.6
IPR006867Domain of unknown function DUF632PFAMPF04782DUF632coord: 293..597
e-value: 2.2E-98
score: 329.6

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh01G001220.1CmoCh01G001220.1mRNA