CmoCh01G000700 (gene) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh01G000700
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
DescriptionPhospholipase D
LocationCmo_Chr01: 288813 .. 291750 (-)
RNA-Seq ExpressionCmoCh01G000700
SyntenyCmoCh01G000700
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TCTTTCTTTCCTTCACTCTCCTACAAAATTGTTTCAAGTTATGACAGTTCAGGGAAAGCATAAGTTCTTCCATGGCACGCTCGAAGTTACCGTCTTCCATGCAACTACATACACACCGTCATCACCACTGGATGTAAGTCTTAGATCATAAGAATGAGGTTTAAATTATAAGTTTAGTCTCTGAACTTTCGTGGTTGTGTTTATAGGTCTATAAACTTTCAACTTTTTGTCTAATACGTCGAATAATACAATTTCTATATCGTCACTAGAATAATATAACAGTTTTTTCTCTCTTGTAGTGTATCTTTTCTGGAGGAAAGCAAAGCTATGTGACCATCAAGATAAACAACATAGAGGTTGCTCGAACAAACCATGAATACGACCGTGTTTGGAACCAAACATTCCGAGTTCTATGTGCGCATCCATTGACATCTACCATCACCATTACAATGAGAACAACTCGTTCTGTATTGGGAAGATTCCAGATCCAAGCCCAACAGATTTTGAAAGAATCAAGTTTCATCAATGGATTCTTTCCACTTCTTATGGAAAATGGGAAGCCGAGTCCAGAGCTCAGACTTAGATTCATGCTATGGTTTCAACCGGCAGTATATGAACTAAGTTGGAAAAAGGTGCTGGAAAATGGAGAGTACAAGGGGCTGAGAAATGCAACGTTTCCTCTAAGGTCCAACTGTCATGTGACTCTATATCAAGATGCTCACCATGTCTCTACGTTTCAACCTCCGTTTCACGGTTCGAGCGCGCCAAGAAGGCTATGGGAAGATGTGTATAAGGCCATAGATAATGCAAAGCATTTGGTTTACATTGCAGGCTGGTCCTTCAATCCAAAGATGGTGCTGGTAAATACAAACAATCACCTATCTTTGTTAAAGTACAAACTTTAGTCCATGAATTTTTAACCGCTAATACTCTTTTGGTGATCAGGTAAGAGACCCTCAAACTCATATACCCTATGCCTTGGGAGTAAAGCTTGGTGAGTTGTTGAAGCAGAAGGCAGAGGAAGGAGTAGCAGTGAGACTAATGATATGGGACGACGAGACATCCCTTCCCATCATCAAGAATGCTGGGATAATGAAAACACATGATGAAGATGCTCGGGCATATTTCTCGAACTCGAAAGTTGTATGTAGGTTGTGCCCCAAATTACATCCTATGTCTCCACCAATTTTCTCTCATCATCAGAAGACCATAACAGTAGACGCTCAAACTCACATCAACGCAAGGAACAGGGAGATTATGAGTTTCATTGGGGGTCTAGATCTTTGTGATGGTCGCTATGACACAGAACAACATTCATTGTTCCATACTTTGAACACAGAATCCCACTGCTGTGATTTCTACCAAACGAGTATATCAGGAGCCAAGCTTCAAAAAGGAGGGCCAAGAGAGCCATGGCATGATGTTCATGCTCGCGTAACAGGTGAAGCTGCTTGGGATATACTAACAAACTTTGAGCAACGATGGACTAAGCAATCAGATGCTTCGTTGCTAGTCCCAACAAGCATCTTACTAAAACTGATGCCCCCACTTGAATCAAACACAAACCCACAAAAGGATTGGAACGTACAAGTTTTTCGATCTATCAACCACTTGTCTGCCAGCCAAGTTTTCAGAAACTTGACGGTTGAACGTACCATCCACGAGGCATATGTCGAAGCTATCAGAAGAGCCGAGAGATTTATTTACATCGAAAACCAATATTTCATAGGAGGGTGTCACTTGTGGGACAAAGATCAACACTGCGGATGCACGAATTTAATACCGATCGAGATTGCGCTCAAGGTGGCTAATAAGATCAAGGCAAGGGAGAAGTTTGCAGTTTATGTAGTGATTCCAATGTGGCCAGAAGGGCCACCAGAGAGTGAATCAGTAGAGGATATGCTACATTGGACCAGGCAGACAATGACAATGATGTATAGATTGATTGGAGAAGCAATCCAAGAAACTGGGGAGAAGGCCCATCCAAGAGACTACTTGAATTTCTTTTGCCTTGCAAACAGAGAAGAAGAGAGAAAGTGGGACTTTGTTCCCCCTCACAGTCCACAACATGCAACAGAATACTGGAGTGCCCAACACCATAGAAGGTTTATGGTCTATGTCCATTCGAAGGTCATGATAGGTAAGTTTTCAAGTATTCCGAACATCGTTTCAAAGGGGCATTTTAACAAACATGTAGAGGGCAGTTTTAATAACATAATAACGCAAAAGAAACAAAGAATTGATTCAAGAGTTTTGGATCTCATTTCAACAAACACAAATGACAAATCTATTATCCATAAAAGTGATGATGGGTTCATTACATATTCAAATTACAACGATTTAAACCAAAAGTGTAGAAGAAGTAGAAACTAACCATAAATATGTGGGTGTTTGCAGTGGACGATTTGTACATTCTGATCGGATCCGCAAACGTGAACCAAAGATCCATGGACGGAGAACGAGATACAGAGATAGCGATCGGATGCTACCAACTAGAAAACGACGGAAAAGAATTGCCAAATGGAAGAGACATTTCAACATTCCGCTTGTCGCTTTGGTACGAACATACTCAACGATTCGAAGAGGTGTTCTTAAACCCCGAAAACTTGCAATGCGTAGAAAGGGTTCGTTCAATAGCAGACGAATCATGGAAAATTTACAGTGGAGAAGAAGTTGCAGACATGAAAGGTGTTCATCTCGTAACGTACCCTGTGAAGGTAAAACAGGACGGATCGATTGAGGATTTGGAAGAAAATGGAGGCCATTTTCCTGATACTAAATGCCCCATTAAAGGAAGAAGATCAATGTTCTTGCCGCCCATTTTCACGACCTAATTTTAATGGAGACTTGTATGGCGATGACCAAGCTCTGAGTTTGCATGTACTTGCTCGAAGATCCATGAACGAGAAGACCCAACTGGGGAATTTGTGGTTTGGAG

mRNA sequence

TCTTTCTTTCCTTCACTCTCCTACAAAATTGTTTCAAGTTATGACAGTTCAGGGAAAGCATAAGTTCTTCCATGGCACGCTCGAAGTTACCGTCTTCCATGCAACTACATACACACCGTCATCACCACTGGATTGTATCTTTTCTGGAGGAAAGCAAAGCTATGTGACCATCAAGATAAACAACATAGAGGTTGCTCGAACAAACCATGAATACGACCGTGTTTGGAACCAAACATTCCGAGTTCTATGTGCGCATCCATTGACATCTACCATCACCATTACAATGAGAACAACTCGTTCTGTATTGGGAAGATTCCAGATCCAAGCCCAACAGATTTTGAAAGAATCAAGTTTCATCAATGGATTCTTTCCACTTCTTATGGAAAATGGGAAGCCGAGTCCAGAGCTCAGACTTAGATTCATGCTATGGTTTCAACCGGCAGTATATGAACTAAGTTGGAAAAAGGTGCTGGAAAATGGAGAGTACAAGGGGCTGAGAAATGCAACGTTTCCTCTAAGGTCCAACTGTCATGTGACTCTATATCAAGATGCTCACCATGTCTCTACGTTTCAACCTCCGTTTCACGGTTCGAGCGCGCCAAGAAGGCTATGGGAAGATGTGTATAAGGCCATAGATAATGCAAAGCATTTGGTTTACATTGCAGGCTGGTCCTTCAATCCAAAGATGGTGCTGGTAAGAGACCCTCAAACTCATATACCCTATGCCTTGGGAGTAAAGCTTGGTGAGTTGTTGAAGCAGAAGGCAGAGGAAGGAGTAGCAGTGAGACTAATGATATGGGACGACGAGACATCCCTTCCCATCATCAAGAATGCTGGGATAATGAAAACACATGATGAAGATGCTCGGGCATATTTCTCGAACTCGAAAGTTGTATGTAGGTTGTGCCCCAAATTACATCCTATGTCTCCACCAATTTTCTCTCATCATCAGAAGACCATAACAGTAGACGCTCAAACTCACATCAACGCAAGGAACAGGGAGATTATGAGTTTCATTGGGGGTCTAGATCTTTGTGATGGTCGCTATGACACAGAACAACATTCATTGTTCCATACTTTGAACACAGAATCCCACTGCTGTGATTTCTACCAAACGAGTATATCAGGAGCCAAGCTTCAAAAAGGAGGGCCAAGAGAGCCATGGCATGATGTTCATGCTCGCGTAACAGGTGAAGCTGCTTGGGATATACTAACAAACTTTGAGCAACGATGGACTAAGCAATCAGATGCTTCGTTGCTAGTCCCAACAAGCATCTTACTAAAACTGATGCCCCCACTTGAATCAAACACAAACCCACAAAAGGATTGGAACGTACAAGTTTTTCGATCTATCAACCACTTGTCTGCCAGCCAAGTTTTCAGAAACTTGACGGTTGAACGTACCATCCACGAGGCATATGTCGAAGCTATCAGAAGAGCCGAGAGATTTATTTACATCGAAAACCAATATTTCATAGGAGGGTGTCACTTGTGGGACAAAGATCAACACTGCGGATGCACGAATTTAATACCGATCGAGATTGCGCTCAAGGTGGCTAATAAGATCAAGGCAAGGGAGAAGTTTGCAGTTTATGTAGTGATTCCAATGTGGCCAGAAGGGCCACCAGAGAGTGAATCAGTAGAGGATATGCTACATTGGACCAGGCAGACAATGACAATGATGTATAGATTGATTGGAGAAGCAATCCAAGAAACTGGGGAGAAGGCCCATCCAAGAGACTACTTGAATTTCTTTTGCCTTGCAAACAGAGAAGAAGAGAGAAAGTGGGACTTTGTTCCCCCTCACAGTCCACAACATGCAACAGAATACTGGAGTGCCCAACACCATAGAAGGTTTATGGTCTATGTCCATTCGAAGGTCATGATAGTGGACGATTTGTACATTCTGATCGGATCCGCAAACGTGAACCAAAGATCCATGGACGGAGAACGAGATACAGAGATAGCGATCGGATGCTACCAACTAGAAAACGACGGAAAAGAATTGCCAAATGGAAGAGACATTTCAACATTCCGCTTGTCGCTTTGGTACGAACATACTCAACGATTCGAAGAGGTGTTCTTAAACCCCGAAAACTTGCAATGCGTAGAAAGGGTTCGTTCAATAGCAGACGAATCATGGAAAATTTACAGTGGAGAAGAAGTTGCAGACATGAAAGGTGTTCATCTCGTAACGTACCCTGTGAAGGTAAAACAGGACGGATCGATTGAGGATTTGGAAGAAAATGGAGGCCATTTTCCTGATACTAAATGCCCCATTAAAGGAAGAAGATCAATGTTCTTGCCGCCCATTTTCACGACCTAATTTTAATGGAGACTTGTATGGCGATGACCAAGCTCTGAGTTTGCATGTACTTGCTCGAAGATCCATGAACGAGAAGACCCAACTGGGGAATTTGTGGTTTGGAG

Coding sequence (CDS)

ATGACAGTTCAGGGAAAGCATAAGTTCTTCCATGGCACGCTCGAAGTTACCGTCTTCCATGCAACTACATACACACCGTCATCACCACTGGATTGTATCTTTTCTGGAGGAAAGCAAAGCTATGTGACCATCAAGATAAACAACATAGAGGTTGCTCGAACAAACCATGAATACGACCGTGTTTGGAACCAAACATTCCGAGTTCTATGTGCGCATCCATTGACATCTACCATCACCATTACAATGAGAACAACTCGTTCTGTATTGGGAAGATTCCAGATCCAAGCCCAACAGATTTTGAAAGAATCAAGTTTCATCAATGGATTCTTTCCACTTCTTATGGAAAATGGGAAGCCGAGTCCAGAGCTCAGACTTAGATTCATGCTATGGTTTCAACCGGCAGTATATGAACTAAGTTGGAAAAAGGTGCTGGAAAATGGAGAGTACAAGGGGCTGAGAAATGCAACGTTTCCTCTAAGGTCCAACTGTCATGTGACTCTATATCAAGATGCTCACCATGTCTCTACGTTTCAACCTCCGTTTCACGGTTCGAGCGCGCCAAGAAGGCTATGGGAAGATGTGTATAAGGCCATAGATAATGCAAAGCATTTGGTTTACATTGCAGGCTGGTCCTTCAATCCAAAGATGGTGCTGGTAAGAGACCCTCAAACTCATATACCCTATGCCTTGGGAGTAAAGCTTGGTGAGTTGTTGAAGCAGAAGGCAGAGGAAGGAGTAGCAGTGAGACTAATGATATGGGACGACGAGACATCCCTTCCCATCATCAAGAATGCTGGGATAATGAAAACACATGATGAAGATGCTCGGGCATATTTCTCGAACTCGAAAGTTGTATGTAGGTTGTGCCCCAAATTACATCCTATGTCTCCACCAATTTTCTCTCATCATCAGAAGACCATAACAGTAGACGCTCAAACTCACATCAACGCAAGGAACAGGGAGATTATGAGTTTCATTGGGGGTCTAGATCTTTGTGATGGTCGCTATGACACAGAACAACATTCATTGTTCCATACTTTGAACACAGAATCCCACTGCTGTGATTTCTACCAAACGAGTATATCAGGAGCCAAGCTTCAAAAAGGAGGGCCAAGAGAGCCATGGCATGATGTTCATGCTCGCGTAACAGGTGAAGCTGCTTGGGATATACTAACAAACTTTGAGCAACGATGGACTAAGCAATCAGATGCTTCGTTGCTAGTCCCAACAAGCATCTTACTAAAACTGATGCCCCCACTTGAATCAAACACAAACCCACAAAAGGATTGGAACGTACAAGTTTTTCGATCTATCAACCACTTGTCTGCCAGCCAAGTTTTCAGAAACTTGACGGTTGAACGTACCATCCACGAGGCATATGTCGAAGCTATCAGAAGAGCCGAGAGATTTATTTACATCGAAAACCAATATTTCATAGGAGGGTGTCACTTGTGGGACAAAGATCAACACTGCGGATGCACGAATTTAATACCGATCGAGATTGCGCTCAAGGTGGCTAATAAGATCAAGGCAAGGGAGAAGTTTGCAGTTTATGTAGTGATTCCAATGTGGCCAGAAGGGCCACCAGAGAGTGAATCAGTAGAGGATATGCTACATTGGACCAGGCAGACAATGACAATGATGTATAGATTGATTGGAGAAGCAATCCAAGAAACTGGGGAGAAGGCCCATCCAAGAGACTACTTGAATTTCTTTTGCCTTGCAAACAGAGAAGAAGAGAGAAAGTGGGACTTTGTTCCCCCTCACAGTCCACAACATGCAACAGAATACTGGAGTGCCCAACACCATAGAAGGTTTATGGTCTATGTCCATTCGAAGGTCATGATAGTGGACGATTTGTACATTCTGATCGGATCCGCAAACGTGAACCAAAGATCCATGGACGGAGAACGAGATACAGAGATAGCGATCGGATGCTACCAACTAGAAAACGACGGAAAAGAATTGCCAAATGGAAGAGACATTTCAACATTCCGCTTGTCGCTTTGGTACGAACATACTCAACGATTCGAAGAGGTGTTCTTAAACCCCGAAAACTTGCAATGCGTAGAAAGGGTTCGTTCAATAGCAGACGAATCATGGAAAATTTACAGTGGAGAAGAAGTTGCAGACATGAAAGGTGTTCATCTCGTAACGTACCCTGTGAAGGTAAAACAGGACGGATCGATTGAGGATTTGGAAGAAAATGGAGGCCATTTTCCTGATACTAAATGCCCCATTAAAGGAAGAAGATCAATGTTCTTGCCGCCCATTTTCACGACCTAA

Protein sequence

MTVQGKHKFFHGTLEVTVFHATTYTPSSPLDCIFSGGKQSYVTIKINNIEVARTNHEYDRVWNQTFRVLCAHPLTSTITITMRTTRSVLGRFQIQAQQILKESSFINGFFPLLMENGKPSPELRLRFMLWFQPAVYELSWKKVLENGEYKGLRNATFPLRSNCHVTLYQDAHHVSTFQPPFHGSSAPRRLWEDVYKAIDNAKHLVYIAGWSFNPKMVLVRDPQTHIPYALGVKLGELLKQKAEEGVAVRLMIWDDETSLPIIKNAGIMKTHDEDARAYFSNSKVVCRLCPKLHPMSPPIFSHHQKTITVDAQTHINARNREIMSFIGGLDLCDGRYDTEQHSLFHTLNTESHCCDFYQTSISGAKLQKGGPREPWHDVHARVTGEAAWDILTNFEQRWTKQSDASLLVPTSILLKLMPPLESNTNPQKDWNVQVFRSINHLSASQVFRNLTVERTIHEAYVEAIRRAERFIYIENQYFIGGCHLWDKDQHCGCTNLIPIEIALKVANKIKAREKFAVYVVIPMWPEGPPESESVEDMLHWTRQTMTMMYRLIGEAIQETGEKAHPRDYLNFFCLANREEERKWDFVPPHSPQHATEYWSAQHHRRFMVYVHSKVMIVDDLYILIGSANVNQRSMDGERDTEIAIGCYQLENDGKELPNGRDISTFRLSLWYEHTQRFEEVFLNPENLQCVERVRSIADESWKIYSGEEVADMKGVHLVTYPVKVKQDGSIEDLEENGGHFPDTKCPIKGRRSMFLPPIFTT
Homology
BLAST of CmoCh01G000700 vs. ExPASy Swiss-Prot
Match: Q9C888 (Phospholipase D alpha 4 OS=Arabidopsis thaliana OX=3702 GN=PLDALPHA4 PE=2 SV=1)

HSP 1 Score: 977.2 bits (2525), Expect = 1.0e-283
Identity = 468/779 (60.08%), Postives = 599/779 (76.89%), Query Frame = 0

Query: 1   MTVQGKHKFFHGTLEVTVFHATTYTPSSPLDCIFSGGKQSYVTIKINNIEVARTNHEYDR 60
           M ++ + K+FHGTLE+T+F AT ++P  P +CI +  K +YVTIKIN  +VA+T+ EYDR
Sbjct: 1   MELEEQKKYFHGTLEITIFDATPFSPPFPFNCICTKPKAAYVTIKINKKKVAKTSSEYDR 60

Query: 61  VWNQTFRVLCAHPLT-STITITMRTTRSVLGRFQIQAQQIL-KESSFINGFFPLLMENGK 120
           +WNQTF++LCAHP+T +TITIT++T  SVLGRF+I A+QIL   S+ INGFFPL+ +NG 
Sbjct: 61  IWNQTFQILCAHPVTDTTITITLKTRCSVLGRFRISAEQILTSNSAVINGFFPLIADNGS 120

Query: 121 PSPELRLRFMLWFQPAVYELSWKKVLENGEYKGLRNATFPLRSNCHVTLYQDAHHVSTFQ 180
               L+L+ ++WF+PA  E  W + LE   ++G+RNA+FP RSNC V LYQDAHH +TF 
Sbjct: 121 TKRNLKLKCLMWFRPAYLEPGWCRALEEASFQGIRNASFPQRSNCRVVLYQDAHHKATFD 180

Query: 181 P-----PFHGSSAPRRLWEDVYKAIDNAKHLVYIAGWSFNPKMVLVRDPQTHIPYALGVK 240
           P     PF+     R LWEDVYKAI++A+HLVYIAGW+ NP +VLVRD +T IP+A+GV 
Sbjct: 181 PRVDDVPFNA----RNLWEDVYKAIESARHLVYIAGWALNPNLVLVRDNETEIPHAVGVT 240

Query: 241 LGELLKQKAEEGVAVRLMIWDDETSLPIIKNAGIMKTHDEDARAYFSNSKVVCRLCPKLH 300
           +GELLK+K+EEGVAVR+M+W+DETSLP+IKN G+M+T+ E A AYF N+ VVCRLCP+LH
Sbjct: 241 VGELLKRKSEEGVAVRVMLWNDETSLPMIKNKGVMRTNVERALAYFRNTNVVCRLCPRLH 300

Query: 301 PMSPPIFSHHQKTITVDAQ-THINARNREIMSFIGGLDLCDGRYDTEQHSLFHTLNTESH 360
              P  F+HHQKTIT+D + T+ + + REIMSF+GG DLCDGRYDTE+HSLF TL TE+ 
Sbjct: 301 KKLPTAFAHHQKTITLDTRVTNSSTKEREIMSFLGGFDLCDGRYDTEEHSLFRTLGTEA- 360

Query: 361 CCDFYQTSISGAKLQKGGPREPWHDVHARVTGEAAWDILTNFEQRWTKQSDASLLVPTS- 420
             DFYQTS++GAKL +GGPREPWHD H  V G AAWD+L NFEQRWTKQ + S+LV TS 
Sbjct: 361 --DFYQTSVAGAKLSRGGPREPWHDCHVSVVGGAAWDVLKNFEQRWTKQCNPSVLVNTSG 420

Query: 421 --ILLKLMPPLESNTNPQKDWNVQVFRSINHLSASQVFRNLTVERTIHEAYVEAIRRAER 480
              L+ L  P E N    + WNVQV RSI+H+SA+++ R L VE+++H+ YV AIR+AER
Sbjct: 421 IRNLVNLTGPTEEN---NRKWNVQVLRSIDHISATEMPRGLPVEKSVHDGYVAAIRKAER 480

Query: 481 FIYIENQYFIGGCHLWD--KDQHC-GCTNLIPIEIALKVANKIKAREKFAVYVVIPMWPE 540
           FIYIENQYF+G C  W+   D+ C GCTNLIP+EIALK+A KI+ARE+FAVY+VIPMWPE
Sbjct: 481 FIYIENQYFMGSCDHWESKNDKICSGCTNLIPVEIALKIAAKIRARERFAVYIVIPMWPE 540

Query: 541 GPPESESVEDMLHWTRQTMTMMYRLIGEAIQETGEKAHPRDYLNFFCLANREEERKWDFV 600
           GPPESE+VE++LHWTR+TM+MMY++IGEAI E G+K+HPRDYLNFFCLANREE+R  +F 
Sbjct: 541 GPPESETVEEILHWTRETMSMMYQIIGEAIWEVGDKSHPRDYLNFFCLANREEKRDGEFE 600

Query: 601 PPHSPQHATEYWSAQHHRRFMVYVHSKVMIVDDLYILIGSANVNQRSMDGERDTEIAIGC 660
              SP   T YW+AQ +RRFMVYVHSK+MIVDD YILIGSAN+NQRSMDG RDTEIAIGC
Sbjct: 601 AVSSPHQKTHYWNAQRNRRFMVYVHSKLMIVDDTYILIGSANINQRSMDGCRDTEIAIGC 660

Query: 661 YQLENDGKELPNGRDISTFRLSLWYEHT----QRFEEVFLNPENLQCVERVRSIADESWK 720
           YQ   +     N  +I  +RLSLWYEHT       +     PE+L+CV  +R+I ++ W+
Sbjct: 661 YQTNTN-----NTNEIQAYRLSLWYEHTGGKITADDLSSSEPESLECVRGLRTIGEQMWE 720

Query: 721 IYSGEEVADMKGVHLVTYPVKVKQDGSIEDLEENGGHFPDTKCPIKGRRSMFLPPIFTT 762
           IYSG++V DM G+HLV YP+ V  DG++E++ +  G FPDTK  +KG+RS   PP+ TT
Sbjct: 721 IYSGDKVVDMLGIHLVAYPISVTGDGAVEEVGD--GCFPDTKTLVKGKRSKMFPPVLTT 762

BLAST of CmoCh01G000700 vs. ExPASy Swiss-Prot
Match: Q43270 (Phospholipase D alpha 1 OS=Zea mays OX=4577 GN=PLD1 PE=2 SV=1)

HSP 1 Score: 625.5 bits (1612), Expect = 7.6e-178
Identity = 333/810 (41.11%), Postives = 475/810 (58.64%), Query Frame = 0

Query: 11  HGTLEVTVFHATTY------TPSSPLDC------------IFSGGKQSYVTIKINNIEVA 70
           HGTL  T+F A +       T  +P               +  G  + Y T+ +    V 
Sbjct: 7   HGTLHATIFEAESLSNPHRATGGAPKFIRKLVEGIEDTVGVGKGATKIYATVDLEKARVG 66

Query: 71  RT----NHEYDRVWNQTFRVLCAH---PLTSTITITMRTTRSVLGRFQIQAQQILKESSF 130
           RT    N   +  W ++F + CAH    +  T+ I      S++GR  +  Q +L     
Sbjct: 67  RTRMISNEPVNPRWYESFHIYCAHMAADVIFTVKIDNSIGASLIGRAYLAVQDLLGGEE- 126

Query: 131 INGFFPLLMENGKPSPELRLRFMLWFQPAVYELSWKKVLENGEYKGLRNATFPLRSNCHV 190
           I+ +  +  EN +P  + ++   L +     + +W + + + +Y G+    F  R  C V
Sbjct: 127 IDKWLEISDENREPVGDSKIHVKLQYFDVGKDRNWARGVRSTKYPGVPYTFFSQRQGCKV 186

Query: 191 TLYQDAHHVSTFQPPFHGSSA----PRRLWEDVYKAIDNAKHLVYIAGWSFNPKMVLVRD 250
           TLYQDAH    F P    +      P R WED++ AI  A+HL+YI GWS   ++ LVRD
Sbjct: 187 TLYQDAHVPDNFVPRIQLADGKNYEPHRCWEDIFDAISKAQHLIYITGWSVYTEITLVRD 246

Query: 251 PQTHIPYALGVKLGELLKQKAEEGVAVRLMIWDDETSLPIIKNAGIMKTHDEDARAYFSN 310
                P    V LGELLK+KA EGV V +++WDD TS+ ++K  G+M THDE+   YF  
Sbjct: 247 TNRPKPGG-DVTLGELLKRKASEGVRVLMLVWDDRTSVGLLKKDGLMATHDEETANYFHG 306

Query: 311 SKVVCRLCPKLHPMS---------PPIFSHHQKTITVDAQ-THINARNREIMSFIGGLDL 370
           + V C LCP+    S           +F+HHQK + VD +  +  ++ R I+SFIGG+DL
Sbjct: 307 TDVNCVLCPRNPDDSGSFVQDLQISTMFTHHQKIVVVDHEMPNQGSQQRRIVSFIGGIDL 366

Query: 371 CDGRYDTEQHSLFHTLNTESHCCDFYQTSISGAKLQKGGPREPWHDVHARVTGEAAWDIL 430
           CDGRYDT+ HSLF TL+T  H  DF+Q +  G  ++KGGPREPWHD+H+R+ G  AWD+L
Sbjct: 367 CDGRYDTQYHSLFRTLDTVHH-DDFHQPNFEGGSIKKGGPREPWHDIHSRLEGPIAWDVL 426

Query: 431 TNFEQRWTKQSDASLLVPTSILLKLMPPLESNTNPQ--KDWNVQVFRSINHLSASQV--- 490
            NFEQRW KQ    LLV    L  ++ P      P+  + WNVQ+FRSI+  +A      
Sbjct: 427 YNFEQRWRKQGGKDLLVRLRDLPDIIIPPSPVMFPEDRETWNVQLFRSIDGGAAFGFPET 486

Query: 491 -----------FRNLTVERTIHEAYVEAIRRAERFIYIENQYFIGGCHLWD----KDQHC 550
                       ++  ++R+I +AYV AIRRA+ FIYIENQYF+G  + W     K +  
Sbjct: 487 PEEAARAGLVSGKDQIIDRSIQDAYVNAIRRAKNFIYIENQYFLGSSYGWKPEGIKPEEI 546

Query: 551 GCTNLIPIEIALKVANKIKAREKFAVYVVIPMWPEGPPESESVEDMLHWTRQTMTMMYRL 610
           G  +LIP E++LK+ +KI+A E+F VYVV+PMWPEG PES SV+ +L W R+TM MMY  
Sbjct: 547 GALHLIPKELSLKIVSKIEAGERFTVYVVVPMWPEGVPESASVQAILDWQRRTMEMMYTD 606

Query: 611 IGEAIQETGEKAHPRDYLNFFCLANREEERKWDFVPPHSPQHATEYWSAQHHRRFMVYVH 670
           I +A++  G +A+P+DYL FFCL NRE +++ ++ P   P+  T+Y  AQ  RRFM+YVH
Sbjct: 607 IAQALEANGIEANPKDYLTFFCLGNREVKQEGEYEPEEHPEPDTDYIRAQEARRFMIYVH 666

Query: 671 SKVMIVDDLYILIGSANVNQRSMDGERDTEIAIGCYQLENDGKELPNGRDISTFRLSLWY 730
           +K+MIVDD YI+IGSAN+NQRSMDG RD+EIA+G YQ  +     P    I  FR+SLWY
Sbjct: 667 TKMMIVDDEYIIIGSANINQRSMDGARDSEIAMGAYQPYHLATRQPARGQIHGFRMSLWY 726

Query: 731 EHTQRFEEVFLNPENLQCVERVRSIADESWKIYSGEEVADMKGVHLVTYPVKVKQDGSIE 762
           EH    E+VF  PE+++CV++V  +A++ W +YS +++      HL++YP+ V  DGS+ 
Sbjct: 727 EHLGMLEDVFQRPESVECVQKVNEVAEKYWDLYSSDDLEQDLPGHLLSYPIGVTADGSVT 786

BLAST of CmoCh01G000700 vs. ExPASy Swiss-Prot
Match: Q43007 (Phospholipase D alpha 1 OS=Oryza sativa subsp. japonica OX=39947 GN=PLD1 PE=1 SV=2)

HSP 1 Score: 621.7 bits (1602), Expect = 1.1e-176
Identity = 334/811 (41.18%), Postives = 477/811 (58.82%), Query Frame = 0

Query: 11  HGTLEVTVFHATTY------TPSSPLDC------------IFSGGKQSYVTIKINNIEVA 70
           HGTL  T+F A +       + S+P               +  G  + Y TI +    V 
Sbjct: 7   HGTLHATIFEAASLSNPHRASGSAPKFIRKFVEGIEDTVGVGKGATKVYSTIDLEKARVG 66

Query: 71  R----TNHEYDRVWNQTFRVLCAHPLTSTITITMRTTRSV----LGRFQIQAQQILKESS 130
           R    TN   +  W ++F + CAH + S +  T++    +    +GR  +  Q++L    
Sbjct: 67  RTRMITNEPINPRWYESFHIYCAH-MASNVIFTVKIDNPIGATNIGRAYLPVQELLNGEE 126

Query: 131 FINGFFPLLMENGKPSPELRLRFMLWFQPAVYELSWKKVLENGEYKGLRNATFPLRSNCH 190
            I+ +  +   N +P  E ++   L +     + +W + + + +Y G+    F  R  C 
Sbjct: 127 -IDRWLDICDNNREPVGESKIHVKLQYFDVSKDRNWARGVRSTKYPGVPYTFFSQRQGCK 186

Query: 191 VTLYQDAHHVSTFQPPFHGSSA----PRRLWEDVYKAIDNAKHLVYIAGWSFNPKMVLVR 250
           VTLYQDAH    F P    +      P R WED++ AI NA+HL+YI GWS   ++ LVR
Sbjct: 187 VTLYQDAHVPDNFIPKIPLADGKNYEPHRCWEDIFDAISNAQHLIYITGWSVYTEITLVR 246

Query: 251 DPQTHIPYALGVKLGELLKQKAEEGVAVRLMIWDDETSLPIIKNAGIMKTHDEDARAYFS 310
           D     P    V LGELLK+KA EGV V +++WDD TS+ ++K  G+M THDE+   YF 
Sbjct: 247 DSNRPKPGG-DVTLGELLKKKASEGVRVLMLVWDDRTSVGLLKRDGLMATHDEETENYFH 306

Query: 311 NSKVVCRLCPKLHPMSPPI---------FSHHQKTITVDAQ-THINARNREIMSFIGGLD 370
            S V C LCP+    S  I         F+HHQK + VD +  +  ++ R I+SF+GGLD
Sbjct: 307 GSDVNCVLCPRNPDDSGSIVQDLSISTMFTHHQKIVVVDHELPNQGSQQRRIVSFVGGLD 366

Query: 371 LCDGRYDTEQHSLFHTLNTESHCCDFYQTSISGAKLQKGGPREPWHDVHARVTGEAAWDI 430
           LCDGRYDT+ HSLF TL++ +H  DF+Q + + A ++KGGPREPWHD+H+R+ G  AWD+
Sbjct: 367 LCDGRYDTQYHSLFRTLDS-THHDDFHQPNFATASIKKGGPREPWHDIHSRLEGPIAWDV 426

Query: 431 LTNFEQRWTKQSDASLLVPTSILLKLMPPLESNTNPQ--KDWNVQVFRSINHLSASQV-- 490
           L NFEQRW KQ    LL+    L   + P      P+  + WNVQ+FRSI+  +A     
Sbjct: 427 LYNFEQRWRKQGGKDLLLQLRDLSDTIIPPSPVMFPEDRETWNVQLFRSIDGGAAFGFPD 486

Query: 491 ------------FRNLTVERTIHEAYVEAIRRAERFIYIENQYFIGGCHLWD----KDQH 550
                        ++  ++R+I +AY+ AIRRA+ FIYIENQYF+G  + W     K + 
Sbjct: 487 TPEEAAKAGLVSGKDQIIDRSIQDAYIHAIRRAKNFIYIENQYFLGSSYAWKPEGIKPED 546

Query: 551 CGCTNLIPIEIALKVANKIKAREKFAVYVVIPMWPEGPPESESVEDMLHWTRQTMTMMYR 610
            G  +LIP E+ALKV +KI+A E+F VYVV+PMWPEG PES SV+ +L W R+TM MMY 
Sbjct: 547 IGALHLIPKELALKVVSKIEAGERFTVYVVVPMWPEGVPESGSVQAILDWQRRTMEMMYT 606

Query: 611 LIGEAIQETGEKAHPRDYLNFFCLANREEERKWDFVPPHSPQHATEYWSAQHHRRFMVYV 670
            I EA+Q  G +A+P+DYL FFCL NRE ++  ++ P   P+  T+Y  AQ  RRFM+YV
Sbjct: 607 DITEALQAKGIEANPKDYLTFFCLGNREVKQAGEYQPEEQPEADTDYSRAQEARRFMIYV 666

Query: 671 HSKVMIVDDLYILIGSANVNQRSMDGERDTEIAIGCYQLENDGKELPNGRDISTFRLSLW 730
           H+K+MIVDD YI+IGSAN+NQRSMDG RD+EIA+G YQ  +     P    I  FR++LW
Sbjct: 667 HTKMMIVDDEYIIIGSANINQRSMDGARDSEIAMGGYQPYHLATRQPARGQIHGFRMALW 726

Query: 731 YEHTQRFEEVFLNPENLQCVERVRSIADESWKIYSGEEVADMKGVHLVTYPVKVKQDGSI 762
           YEH    ++VF  PE+L+CV++V  IA++ W +YS +++      HL++YP+ V  DG +
Sbjct: 727 YEHLGMLDDVFQRPESLECVQKVNRIAEKYWDMYSSDDLQQDLPGHLLSYPIGVASDGVV 786

BLAST of CmoCh01G000700 vs. ExPASy Swiss-Prot
Match: P93400 (Phospholipase D alpha 1 OS=Nicotiana tabacum OX=4097 GN=PLD1 PE=2 SV=2)

HSP 1 Score: 607.4 bits (1565), Expect = 2.1e-172
Identity = 326/808 (40.35%), Postives = 472/808 (58.42%), Query Frame = 0

Query: 11  HGTLEVTVFHATTYTP----------SSPLDCIFSGGKQS---YVTIKINNIEVART--- 70
           HGTL VT++                    ++     GK +   Y T+ +    V RT   
Sbjct: 7   HGTLHVTIYEVDNLQKEGGGHFFSKIKEHVEETIGFGKGTPAIYATVDLEKARVGRTRKI 66

Query: 71  -NHEYDRVWNQTFRVLCAHPLTSTITITMRTTR----SVLGRFQIQAQQILKESSFINGF 130
            N   +  W ++F + CAH + S +  T++       +++GR  +  +++L E   I+ +
Sbjct: 67  KNEPNNPRWYESFHIYCAH-MASNVIFTVKDDNPIGATLIGRAYVPVEELL-EGEEIDKW 126

Query: 131 FPLLMENGKPSPE-LRLRFMLWFQPAVYELSWKKVLENGEYKGLRNATFPLRSNCHVTLY 190
             +L     P  E  ++   L F     + +W++ + + +Y G+    F  R+ C V+LY
Sbjct: 127 VEILDREMNPIAEGSKIHVKLQFFDVSRDPNWERGIRSSKYPGVPYTFFAQRTGCRVSLY 186

Query: 191 QDAHHVSTFQP--PFHGSS--APRRLWEDVYKAIDNAKHLVYIAGWSFNPKMVLVRDPQT 250
           QDAH    F P  P  G     P R WED++ AI NAKHL+YI GWS   ++ LVRD + 
Sbjct: 187 QDAHVPDNFIPKIPLSGGKYYEPHRCWEDIFDAIINAKHLIYITGWSVYTEITLVRDSRR 246

Query: 251 HIPYALGVKLGELLKQKAEEGVAVRLMIWDDETSLPIIKNAGIMKTHDEDARAYFSNSKV 310
             P    + LGELLK+KA EGV V +++WDD TS+ ++K  G+M THD++   +F  ++V
Sbjct: 247 QKPGG-DITLGELLKKKASEGVKVLMLVWDDRTSVGLLKKDGLMATHDQETEQFFQGTEV 306

Query: 311 VCRLCPKLHPMSPPI---------FSHHQKTITVDAQTHI-NARNREIMSFIGGLDLCDG 370
            C LCP+       I         F+HHQK + VD++     +  R I+SF+GG+DLCDG
Sbjct: 307 NCVLCPRNPDDGGSIVQSLQIGTMFTHHQKIVVVDSELPSGESEKRRILSFVGGIDLCDG 366

Query: 371 RYDTEQHSLFHTLNTESHCCDFYQTSISGAKLQKGGPREPWHDVHARVTGEAAWDILTNF 430
           RYDT  HSLF TL+T +H  DF+Q +     + KGGPREPWHD+H+R+ G  AWD+L NF
Sbjct: 367 RYDTPFHSLFRTLDT-AHHDDFHQPNFPDGAITKGGPREPWHDIHSRLEGPIAWDVLFNF 426

Query: 431 EQRWTKQSDASLLVPTSIL--LKLMPPLESNTNPQKDWNVQVFRSINHLSASQV------ 490
           EQRW KQ    +LV    L  + + P    + +  + WNVQ+FRSI+  +A         
Sbjct: 427 EQRWRKQGGKDVLVNFRELDDIIIPPSPVMHLDDSETWNVQLFRSIDEGAAFGFPETPED 486

Query: 491 --------FRNLTVERTIHEAYVEAIRRAERFIYIENQYFIGGCHLWDKD----QHCGCT 550
                     +  ++R+I +AY+ AIRRA+ FIYIENQYF+G  + W  D    +  G  
Sbjct: 487 AAKAGLVSGXDNIIDRSIQDAYIHAIRRAKNFIYIENQYFLGSSYDWQSDDIKVEDIGAL 546

Query: 551 NLIPIEIALKVANKIKAREKFAVYVVIPMWPEGPPESESVEDMLHWTRQTMTMMYRLIGE 610
           ++IP E+ALK+ +KI+A E+F VYVV+PMWPEG PES SV+ +L W R+TM MMY+ I +
Sbjct: 547 HVIPKELALKIVSKIEAGERFTVYVVVPMWPEGIPESASVQAILDWQRRTMEMMYKHIVQ 606

Query: 611 AIQETGEKAHPRDYLNFFCLANREEERKWDFVPPHSPQHATEYWSAQHHRRFMVYVHSKV 670
           A+   G +  PR+YL FFC+ NRE ++   + P  +P+  ++Y  AQ  RRFM+YVHSK+
Sbjct: 607 ALNAKGIEEDPRNYLTFFCIGNREVKKSGAYEPSETPEPDSDYIRAQEARRFMIYVHSKM 666

Query: 671 MIVDDLYILIGSANVNQRSMDGERDTEIAIGCYQLENDGKELPNGRDISTFRLSLWYEHT 730
           MIVDD YI++GSAN+NQRSMDG RD+EIA+G YQ  +     P    I  FR++LWYEH 
Sbjct: 667 MIVDDEYIIVGSANINQRSMDGARDSEIAMGAYQPHHLATREPARGQIHGFRMALWYEHL 726

Query: 731 QRFEEVFLNPENLQCVERVRSIADESWKIYSGEEV-ADMKGVHLVTYPVKVKQDGSIEDL 762
              +E FL+PE+ +CV +V  +AD+ W +YS E +  D+ G HL+ YP+ V  +G + +L
Sbjct: 727 GMLDETFLHPESEECVSKVNRMADKYWDLYSSESLERDLPG-HLLRYPIGVASEGDVTEL 786

BLAST of CmoCh01G000700 vs. ExPASy Swiss-Prot
Match: Q70EW5 (Phospholipase D alpha 1 OS=Cynara cardunculus OX=4265 GN=PLD1 PE=1 SV=2)

HSP 1 Score: 606.7 bits (1563), Expect = 3.7e-172
Identity = 324/808 (40.10%), Postives = 471/808 (58.29%), Query Frame = 0

Query: 11  HGTLEVTVFHATTYTPSSPLDC-------------IFSGGKQSYVTIKINNIEVART--- 70
           HGTL VTV+           +                 G  + Y TI +    V RT   
Sbjct: 7   HGTLHVTVYEVDKLREGGGPNVFGKLMANIQETVGFGEGTPKIYATIDLEKSRVGRTRMI 66

Query: 71  -NHEYDRVWNQTFRVLCAHPLTSTITITMRTTR----SVLGRFQIQAQQILKESSFINGF 130
            N   +  W ++F + CAH   S I  T++       ++LGR  +  +++L +   ++ +
Sbjct: 67  ENEPQNPRWYESFHIYCAHH-ASNIIFTVKDDNPIGATLLGRAYMPVRELL-DGDEVDKW 126

Query: 131 FPLLMENGKPSPE-LRLRFMLWFQPAVYELSWKKVLENGEYKGLRNATFPLRSNCHVTLY 190
             ++ E+  P+P   ++   L +     + +W + ++ G+Y G+    F  R  C V+LY
Sbjct: 127 IEIMDEDNNPTPAGSKIHVKLQYFDVTQDRNWDRGIKTGKYPGVPYTFFAQRQGCRVSLY 186

Query: 191 QDAHHVSTFQPPFHGSSA----PRRLWEDVYKAIDNAKHLVYIAGWSFNPKMVLVRDPQT 250
           QDAH    F P    +      P R WED++ AI +AKH +YI GWS   ++ L+RDP  
Sbjct: 187 QDAHVPDNFIPKISLAGGKYYEPHRCWEDIFDAISDAKHFIYITGWSVYTQIPLIRDPNR 246

Query: 251 HIPYALGVKLGELLKQKAEEGVAVRLMIWDDETSLPIIKNAGIMKTHDEDARAYFSNSKV 310
             P    V LG+LLK+KA+EGV V +++WDD TS+ + K  G+M THDE+   +F ++ V
Sbjct: 247 QKPGG-DVLLGQLLKKKADEGVRVAMLVWDDRTSVNVFKEDGLMATHDEETENFFKDTDV 306

Query: 311 VCRLCPK--------LHPMS-PPIFSHHQKTITVDAQ-THINARNREIMSFIGGLDLCDG 370
            C LCP+        +  +    +F+HHQK + VD +     ++ R +MSF+GG+DLCDG
Sbjct: 307 HCILCPRDPDDGGSIIQDLKVSTMFTHHQKIVVVDHELPRGGSQKRRVMSFVGGIDLCDG 366

Query: 371 RYDTEQHSLFHTLNTESHCCDFYQTSISGAKLQKGGPREPWHDVHARVTGEAAWDILTNF 430
           RYD+  H LF TL++ +H  DF+Q + +GA + KGGPREPWHD+H+RV G  AWD+L NF
Sbjct: 367 RYDSAFHPLFSTLDS-AHHDDFHQPNYAGASIAKGGPREPWHDIHSRVEGPIAWDVLFNF 426

Query: 431 EQRWTKQSDASLLVPTSILLKLMPPLESNT--NPQKDWNVQVFRSIN------------H 490
           EQRW KQ   ++LV    L  ++ P    T  N Q+ WNVQ+FRSI+             
Sbjct: 427 EQRWRKQGGKNVLVDLKQLDDILIPPSPVTFPNDQETWNVQLFRSIDGGAAFGFPDTPEE 486

Query: 491 LSASQVF--RNLTVERTIHEAYVEAIRRAERFIYIENQYFIGGCHLWDKD----QHCGCT 550
            S S +   ++  ++R+I +AY+ AIRRA+ FIYIENQYF+G    W  D       G  
Sbjct: 487 ASKSGLVSGKDNIIDRSIQDAYINAIRRAKHFIYIENQYFLGSSFAWKSDDIDVDEVGAL 546

Query: 551 NLIPIEIALKVANKIKAREKFAVYVVIPMWPEGPPESESVEDMLHWTRQTMTMMYRLIGE 610
           +LIP E++LK+  KI+  EKF VY+V+PMWPEG PE+ SV+ +L W R+TM MMY+ I +
Sbjct: 547 HLIPKELSLKIVTKIQEGEKFIVYIVVPMWPEGIPENGSVQAILDWQRRTMEMMYKDIVD 606

Query: 611 AIQETGEKAHPRDYLNFFCLANREEERKWDFVPPHSPQHATEYWSAQHHRRFMVYVHSKV 670
           A+Q+ G    PR+YL FFCL NRE ++  ++ P  +P+  + Y  AQ +RRFM+YVHSK+
Sbjct: 607 ALQDKGLDDDPREYLTFFCLGNREAKKSGEYEPTEAPEPDSGYLHAQENRRFMIYVHSKM 666

Query: 671 MIVDDLYILIGSANVNQRSMDGERDTEIAIGCYQLENDGKELPNGRDISTFRLSLWYEHT 730
           MIVDD YI++GSAN+NQRSMDG RD+EIA+G YQ  +   + P    +  FR++LWYEH 
Sbjct: 667 MIVDDEYIIVGSANINQRSMDGARDSEIAMGAYQPYHLATQTPARGHVHGFRMALWYEHL 726

Query: 731 QRFEEVFLNPENLQCVERVRSIADESWKIYSGEEV-ADMKGVHLVTYPVKVKQDGSIEDL 762
              ++ F  PEN  CV +   +AD+ W +Y+ E++  D+ G HL+ YPV V + G I +L
Sbjct: 727 GMLDDSFERPENKDCVNKANEMADKCWDLYASEDLDRDLPG-HLLRYPVGVTRKGDITEL 786

BLAST of CmoCh01G000700 vs. ExPASy TrEMBL
Match: A0A6J1G9V5 (Phospholipase D OS=Cucurbita moschata OX=3662 GN=LOC111452112 PE=3 SV=1)

HSP 1 Score: 1603.6 bits (4151), Expect = 0.0e+00
Identity = 761/761 (100.00%), Postives = 761/761 (100.00%), Query Frame = 0

Query: 1   MTVQGKHKFFHGTLEVTVFHATTYTPSSPLDCIFSGGKQSYVTIKINNIEVARTNHEYDR 60
           MTVQGKHKFFHGTLEVTVFHATTYTPSSPLDCIFSGGKQSYVTIKINNIEVARTNHEYDR
Sbjct: 62  MTVQGKHKFFHGTLEVTVFHATTYTPSSPLDCIFSGGKQSYVTIKINNIEVARTNHEYDR 121

Query: 61  VWNQTFRVLCAHPLTSTITITMRTTRSVLGRFQIQAQQILKESSFINGFFPLLMENGKPS 120
           VWNQTFRVLCAHPLTSTITITMRTTRSVLGRFQIQAQQILKESSFINGFFPLLMENGKPS
Sbjct: 122 VWNQTFRVLCAHPLTSTITITMRTTRSVLGRFQIQAQQILKESSFINGFFPLLMENGKPS 181

Query: 121 PELRLRFMLWFQPAVYELSWKKVLENGEYKGLRNATFPLRSNCHVTLYQDAHHVSTFQPP 180
           PELRLRFMLWFQPAVYELSWKKVLENGEYKGLRNATFPLRSNCHVTLYQDAHHVSTFQPP
Sbjct: 182 PELRLRFMLWFQPAVYELSWKKVLENGEYKGLRNATFPLRSNCHVTLYQDAHHVSTFQPP 241

Query: 181 FHGSSAPRRLWEDVYKAIDNAKHLVYIAGWSFNPKMVLVRDPQTHIPYALGVKLGELLKQ 240
           FHGSSAPRRLWEDVYKAIDNAKHLVYIAGWSFNPKMVLVRDPQTHIPYALGVKLGELLKQ
Sbjct: 242 FHGSSAPRRLWEDVYKAIDNAKHLVYIAGWSFNPKMVLVRDPQTHIPYALGVKLGELLKQ 301

Query: 241 KAEEGVAVRLMIWDDETSLPIIKNAGIMKTHDEDARAYFSNSKVVCRLCPKLHPMSPPIF 300
           KAEEGVAVRLMIWDDETSLPIIKNAGIMKTHDEDARAYFSNSKVVCRLCPKLHPMSPPIF
Sbjct: 302 KAEEGVAVRLMIWDDETSLPIIKNAGIMKTHDEDARAYFSNSKVVCRLCPKLHPMSPPIF 361

Query: 301 SHHQKTITVDAQTHINARNREIMSFIGGLDLCDGRYDTEQHSLFHTLNTESHCCDFYQTS 360
           SHHQKTITVDAQTHINARNREIMSFIGGLDLCDGRYDTEQHSLFHTLNTESHCCDFYQTS
Sbjct: 362 SHHQKTITVDAQTHINARNREIMSFIGGLDLCDGRYDTEQHSLFHTLNTESHCCDFYQTS 421

Query: 361 ISGAKLQKGGPREPWHDVHARVTGEAAWDILTNFEQRWTKQSDASLLVPTSILLKLMPPL 420
           ISGAKLQKGGPREPWHDVHARVTGEAAWDILTNFEQRWTKQSDASLLVPTSILLKLMPPL
Sbjct: 422 ISGAKLQKGGPREPWHDVHARVTGEAAWDILTNFEQRWTKQSDASLLVPTSILLKLMPPL 481

Query: 421 ESNTNPQKDWNVQVFRSINHLSASQVFRNLTVERTIHEAYVEAIRRAERFIYIENQYFIG 480
           ESNTNPQKDWNVQVFRSINHLSASQVFRNLTVERTIHEAYVEAIRRAERFIYIENQYFIG
Sbjct: 482 ESNTNPQKDWNVQVFRSINHLSASQVFRNLTVERTIHEAYVEAIRRAERFIYIENQYFIG 541

Query: 481 GCHLWDKDQHCGCTNLIPIEIALKVANKIKAREKFAVYVVIPMWPEGPPESESVEDMLHW 540
           GCHLWDKDQHCGCTNLIPIEIALKVANKIKAREKFAVYVVIPMWPEGPPESESVEDMLHW
Sbjct: 542 GCHLWDKDQHCGCTNLIPIEIALKVANKIKAREKFAVYVVIPMWPEGPPESESVEDMLHW 601

Query: 541 TRQTMTMMYRLIGEAIQETGEKAHPRDYLNFFCLANREEERKWDFVPPHSPQHATEYWSA 600
           TRQTMTMMYRLIGEAIQETGEKAHPRDYLNFFCLANREEERKWDFVPPHSPQHATEYWSA
Sbjct: 602 TRQTMTMMYRLIGEAIQETGEKAHPRDYLNFFCLANREEERKWDFVPPHSPQHATEYWSA 661

Query: 601 QHHRRFMVYVHSKVMIVDDLYILIGSANVNQRSMDGERDTEIAIGCYQLENDGKELPNGR 660
           QHHRRFMVYVHSKVMIVDDLYILIGSANVNQRSMDGERDTEIAIGCYQLENDGKELPNGR
Sbjct: 662 QHHRRFMVYVHSKVMIVDDLYILIGSANVNQRSMDGERDTEIAIGCYQLENDGKELPNGR 721

Query: 661 DISTFRLSLWYEHTQRFEEVFLNPENLQCVERVRSIADESWKIYSGEEVADMKGVHLVTY 720
           DISTFRLSLWYEHTQRFEEVFLNPENLQCVERVRSIADESWKIYSGEEVADMKGVHLVTY
Sbjct: 722 DISTFRLSLWYEHTQRFEEVFLNPENLQCVERVRSIADESWKIYSGEEVADMKGVHLVTY 781

Query: 721 PVKVKQDGSIEDLEENGGHFPDTKCPIKGRRSMFLPPIFTT 762
           PVKVKQDGSIEDLEENGGHFPDTKCPIKGRRSMFLPPIFTT
Sbjct: 782 PVKVKQDGSIEDLEENGGHFPDTKCPIKGRRSMFLPPIFTT 822

BLAST of CmoCh01G000700 vs. ExPASy TrEMBL
Match: A0A6J1KAT1 (Phospholipase D OS=Cucurbita maxima OX=3661 GN=LOC111492613 PE=3 SV=1)

HSP 1 Score: 1586.6 bits (4107), Expect = 0.0e+00
Identity = 752/761 (98.82%), Postives = 757/761 (99.47%), Query Frame = 0

Query: 1   MTVQGKHKFFHGTLEVTVFHATTYTPSSPLDCIFSGGKQSYVTIKINNIEVARTNHEYDR 60
           MTVQG+H FFHGTLEVTVFHATTYTPSSPLDCIFSGGKQSYVTIKINNIEVARTNHEYDR
Sbjct: 1   MTVQGEHTFFHGTLEVTVFHATTYTPSSPLDCIFSGGKQSYVTIKINNIEVARTNHEYDR 60

Query: 61  VWNQTFRVLCAHPLTSTITITMRTTRSVLGRFQIQAQQILKESSFINGFFPLLMENGKPS 120
           VWNQTFRVLCAHPLTSTITITMRTTRSVLGRFQIQAQQILKESSFINGFFPLLMENGKPS
Sbjct: 61  VWNQTFRVLCAHPLTSTITITMRTTRSVLGRFQIQAQQILKESSFINGFFPLLMENGKPS 120

Query: 121 PELRLRFMLWFQPAVYELSWKKVLENGEYKGLRNATFPLRSNCHVTLYQDAHHVSTFQPP 180
           PELRLRFMLWFQPAVYELSWKKVLENGEYKGLRN TFPLRSNCHVTLYQDAHHVSTFQPP
Sbjct: 121 PELRLRFMLWFQPAVYELSWKKVLENGEYKGLRNVTFPLRSNCHVTLYQDAHHVSTFQPP 180

Query: 181 FHGSSAPRRLWEDVYKAIDNAKHLVYIAGWSFNPKMVLVRDPQTHIPYALGVKLGELLKQ 240
           FHGSSAPRRLWEDVYKAIDNAKHLVYIAGWSFNPKMVLVRDPQT IPYALGVKLGELLKQ
Sbjct: 181 FHGSSAPRRLWEDVYKAIDNAKHLVYIAGWSFNPKMVLVRDPQTDIPYALGVKLGELLKQ 240

Query: 241 KAEEGVAVRLMIWDDETSLPIIKNAGIMKTHDEDARAYFSNSKVVCRLCPKLHPMSPPIF 300
           KAEEGVAVRLMIWDDETSLP+IKNAGIMKTHDE+ARAYFSNSKVVCRLCPKLHPMSPPIF
Sbjct: 241 KAEEGVAVRLMIWDDETSLPVIKNAGIMKTHDENARAYFSNSKVVCRLCPKLHPMSPPIF 300

Query: 301 SHHQKTITVDAQTHINARNREIMSFIGGLDLCDGRYDTEQHSLFHTLNTESHCCDFYQTS 360
           SHHQKTITVDAQTHINARNREIMSFIGGLDLCDGRYDTEQHSLFHTLNTESHCCDFYQTS
Sbjct: 301 SHHQKTITVDAQTHINARNREIMSFIGGLDLCDGRYDTEQHSLFHTLNTESHCCDFYQTS 360

Query: 361 ISGAKLQKGGPREPWHDVHARVTGEAAWDILTNFEQRWTKQSDASLLVPTSILLKLMPPL 420
           ISGAKLQKGGPREPWHDVHARVTGEAAWDILTNFEQRWTKQSDASLLVPTSILLKLMPPL
Sbjct: 361 ISGAKLQKGGPREPWHDVHARVTGEAAWDILTNFEQRWTKQSDASLLVPTSILLKLMPPL 420

Query: 421 ESNTNPQKDWNVQVFRSINHLSASQVFRNLTVERTIHEAYVEAIRRAERFIYIENQYFIG 480
           ESNTNPQKDWNVQVFRSI+HLSASQVFRNLTVERTIHEAYVEAIRRAERFIYIENQYFIG
Sbjct: 421 ESNTNPQKDWNVQVFRSIDHLSASQVFRNLTVERTIHEAYVEAIRRAERFIYIENQYFIG 480

Query: 481 GCHLWDKDQHCGCTNLIPIEIALKVANKIKAREKFAVYVVIPMWPEGPPESESVEDMLHW 540
           GCHLWDKDQHCGCTNLIPIEIALKVANKIKAREKFAVYVVIPMWPEGPPESESVEDMLHW
Sbjct: 481 GCHLWDKDQHCGCTNLIPIEIALKVANKIKAREKFAVYVVIPMWPEGPPESESVEDMLHW 540

Query: 541 TRQTMTMMYRLIGEAIQETGEKAHPRDYLNFFCLANREEERKWDFVPPHSPQHATEYWSA 600
           TRQTMTMMYRLIGEAIQETGEKAHPRDYLNFFCLANREEERKWDFVPPHSPQHATEYWSA
Sbjct: 541 TRQTMTMMYRLIGEAIQETGEKAHPRDYLNFFCLANREEERKWDFVPPHSPQHATEYWSA 600

Query: 601 QHHRRFMVYVHSKVMIVDDLYILIGSANVNQRSMDGERDTEIAIGCYQLENDGKELPNGR 660
           QH RRFMVYVHSKVMIVDDLY+LIGSANVNQRSMDGERDTEIAIGCYQLENDGKELPNGR
Sbjct: 601 QHRRRFMVYVHSKVMIVDDLYLLIGSANVNQRSMDGERDTEIAIGCYQLENDGKELPNGR 660

Query: 661 DISTFRLSLWYEHTQRFEEVFLNPENLQCVERVRSIADESWKIYSGEEVADMKGVHLVTY 720
           DISTFRLSLWYEHTQRFEEVFLNPENLQCVERVRSIADESWKIYSGEEVADMKGVHLVTY
Sbjct: 661 DISTFRLSLWYEHTQRFEEVFLNPENLQCVERVRSIADESWKIYSGEEVADMKGVHLVTY 720

Query: 721 PVKVKQDGSIEDLEENGGHFPDTKCPIKGRRSMFLPPIFTT 762
           PVKVKQDGSIEDLEENGGHFPDTKCPIKGRRSMFLPPIFTT
Sbjct: 721 PVKVKQDGSIEDLEENGGHFPDTKCPIKGRRSMFLPPIFTT 761

BLAST of CmoCh01G000700 vs. ExPASy TrEMBL
Match: A0A5D3BW81 (Phospholipase D OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold863G001380 PE=3 SV=1)

HSP 1 Score: 1472.6 bits (3811), Expect = 0.0e+00
Identity = 686/761 (90.14%), Postives = 731/761 (96.06%), Query Frame = 0

Query: 1   MTVQGKHKFFHGTLEVTVFHATTYTPSSPLDCIFSGGKQSYVTIKINNIEVARTNHEYDR 60
           M ++ K KFFHG LEVTVFHAT YTP SPLDC+F+GGK+SYVTIKI+N EVA+T+HE+DR
Sbjct: 1   MAMEVKQKFFHGMLEVTVFHATAYTPPSPLDCLFAGGKRSYVTIKIDNKEVAQTSHEHDR 60

Query: 61  VWNQTFRVLCAHPLTSTITITMRTTRSVLGRFQIQAQQILKESSFINGFFPLLMENGKPS 120
           VWNQTFRVLCA+PLTSTITIT++T RSVLG+F IQAQQILKE+SFINGFFPLLMENGKPS
Sbjct: 61  VWNQTFRVLCAYPLTSTITITLKTARSVLGKFYIQAQQILKEASFINGFFPLLMENGKPS 120

Query: 121 PELRLRFMLWFQPAVYELSWKKVLENGEYKGLRNATFPLRSNCHVTLYQDAHHVSTFQPP 180
           PEL+LRFMLWF+PAVYELSWKK+L NGEYKGLRNATFPLRSNCHVTLYQDAHH+ TFQPP
Sbjct: 121 PELKLRFMLWFKPAVYELSWKKMLANGEYKGLRNATFPLRSNCHVTLYQDAHHLPTFQPP 180

Query: 181 FHGSSAPRRLWEDVYKAIDNAKHLVYIAGWSFNPKMVLVRDPQTHIPYALGVKLGELLKQ 240
           FHGSS PRRLWEDVYKAIDNAKHLVYIAGWSFNPKMVLVRD QT IPYALGVKLGELLKQ
Sbjct: 181 FHGSSTPRRLWEDVYKAIDNAKHLVYIAGWSFNPKMVLVRDSQTDIPYALGVKLGELLKQ 240

Query: 241 KAEEGVAVRLMIWDDETSLPIIKNAGIMKTHDEDARAYFSNSKVVCRLCPKLHPMSPPIF 300
           KAEEGVAVR++IWDDETSLPIIKNAGIM THDEDARAYF +SKV+CRLCPKLHPMSPPIF
Sbjct: 241 KAEEGVAVRILIWDDETSLPIIKNAGIMNTHDEDARAYFLHSKVICRLCPKLHPMSPPIF 300

Query: 301 SHHQKTITVDAQTHINARNREIMSFIGGLDLCDGRYDTEQHSLFHTLNTESHCCDFYQTS 360
           SHHQK I VDAQTHINA+NREIMSFIGGLDLCDGRYDTEQHSLFHTLNTESHC DFYQTS
Sbjct: 301 SHHQKIIIVDAQTHINAQNREIMSFIGGLDLCDGRYDTEQHSLFHTLNTESHCRDFYQTS 360

Query: 361 ISGAKLQKGGPREPWHDVHARVTGEAAWDILTNFEQRWTKQSDASLLVPTSILLKLMPPL 420
           ISGAKLQKGGPREPWHDVHA VTGEAAWDILTNFEQRWTKQSDASLLVPTSILLKLMP L
Sbjct: 361 ISGAKLQKGGPREPWHDVHACVTGEAAWDILTNFEQRWTKQSDASLLVPTSILLKLMPQL 420

Query: 421 ESNTNPQKDWNVQVFRSINHLSASQVFRNLTVERTIHEAYVEAIRRAERFIYIENQYFIG 480
           ESNTNPQKDWNVQVFRSI+HLSASQ+FRN+T+ERTIHEAYVEAIRRAERFIYIENQYFIG
Sbjct: 421 ESNTNPQKDWNVQVFRSIDHLSASQMFRNMTIERTIHEAYVEAIRRAERFIYIENQYFIG 480

Query: 481 GCHLWDKDQHCGCTNLIPIEIALKVANKIKAREKFAVYVVIPMWPEGPPESESVEDMLHW 540
           GCH WD+D+HCGCTNLIPIEIALKVANKIKARE+FAVY+VIPMWPEG PESESVEDMLHW
Sbjct: 481 GCHFWDRDRHCGCTNLIPIEIALKVANKIKARERFAVYIVIPMWPEGSPESESVEDMLHW 540

Query: 541 TRQTMTMMYRLIGEAIQETGEKAHPRDYLNFFCLANREEERKWDFVPPHSPQHATEYWSA 600
           TRQTMTMMYRLIGEAIQETGEKAHPRDYLNFFCLANREEERKWDF+PPHSPQHAT+YW+A
Sbjct: 541 TRQTMTMMYRLIGEAIQETGEKAHPRDYLNFFCLANREEERKWDFIPPHSPQHATQYWNA 600

Query: 601 QHHRRFMVYVHSKVMIVDDLYILIGSANVNQRSMDGERDTEIAIGCYQLENDGKELPNGR 660
           Q HRRFM+YVHSKVMIVDDLYILIGSANVNQRSMDGERDTEIAIGCYQ+ENDG+ELPNGR
Sbjct: 601 QQHRRFMIYVHSKVMIVDDLYILIGSANVNQRSMDGERDTEIAIGCYQIENDGEELPNGR 660

Query: 661 DISTFRLSLWYEHTQRFEEVFLNPENLQCVERVRSIADESWKIYSGEEVADMKGVHLVTY 720
           DIS FRLSLWYEHT+ FEEVFLNPE+L+CV+RVRSI D+SWKIYSGEEV DMKGVHLVTY
Sbjct: 661 DISRFRLSLWYEHTRGFEEVFLNPESLKCVQRVRSIGDKSWKIYSGEEVEDMKGVHLVTY 720

Query: 721 PVKVKQDGSIEDLEENGGHFPDTKCPIKGRRSMFLPPIFTT 762
           PVKVK+DG++EDLEENGGHFPDTKC IKGRRSM LPPIFTT
Sbjct: 721 PVKVKEDGTMEDLEENGGHFPDTKCAIKGRRSMMLPPIFTT 761

BLAST of CmoCh01G000700 vs. ExPASy TrEMBL
Match: A0A1S3BH90 (Phospholipase D OS=Cucumis melo OX=3656 GN=LOC103490014 PE=3 SV=1)

HSP 1 Score: 1472.6 bits (3811), Expect = 0.0e+00
Identity = 686/761 (90.14%), Postives = 731/761 (96.06%), Query Frame = 0

Query: 1   MTVQGKHKFFHGTLEVTVFHATTYTPSSPLDCIFSGGKQSYVTIKINNIEVARTNHEYDR 60
           M ++ K KFFHG LEVTVFHAT YTP SPLDC+F+GGK+SYVTIKI+N EVA+T+HE+DR
Sbjct: 1   MAMEVKQKFFHGMLEVTVFHATAYTPPSPLDCLFAGGKRSYVTIKIDNKEVAQTSHEHDR 60

Query: 61  VWNQTFRVLCAHPLTSTITITMRTTRSVLGRFQIQAQQILKESSFINGFFPLLMENGKPS 120
           VWNQTFRVLCA+PLTSTITIT++T RSVLG+F IQAQQILKE+SFINGFFPLLMENGKPS
Sbjct: 61  VWNQTFRVLCAYPLTSTITITLKTARSVLGKFYIQAQQILKEASFINGFFPLLMENGKPS 120

Query: 121 PELRLRFMLWFQPAVYELSWKKVLENGEYKGLRNATFPLRSNCHVTLYQDAHHVSTFQPP 180
           PEL+LRFMLWF+PAVYELSWKK+L NGEYKGLRNATFPLRSNCHVTLYQDAHH+ TFQPP
Sbjct: 121 PELKLRFMLWFKPAVYELSWKKMLANGEYKGLRNATFPLRSNCHVTLYQDAHHLPTFQPP 180

Query: 181 FHGSSAPRRLWEDVYKAIDNAKHLVYIAGWSFNPKMVLVRDPQTHIPYALGVKLGELLKQ 240
           FHGSS PRRLWEDVYKAIDNAKHLVYIAGWSFNPKMVLVRD QT IPYALGVKLGELLKQ
Sbjct: 181 FHGSSTPRRLWEDVYKAIDNAKHLVYIAGWSFNPKMVLVRDSQTDIPYALGVKLGELLKQ 240

Query: 241 KAEEGVAVRLMIWDDETSLPIIKNAGIMKTHDEDARAYFSNSKVVCRLCPKLHPMSPPIF 300
           KAEEGVAVR++IWDDETSLPIIKNAGIM THDEDARAYF +SKV+CRLCPKLHPMSPPIF
Sbjct: 241 KAEEGVAVRILIWDDETSLPIIKNAGIMNTHDEDARAYFLHSKVICRLCPKLHPMSPPIF 300

Query: 301 SHHQKTITVDAQTHINARNREIMSFIGGLDLCDGRYDTEQHSLFHTLNTESHCCDFYQTS 360
           SHHQK I VDAQTHINA+NREIMSFIGGLDLCDGRYDTEQHSLFHTLNTESHC DFYQTS
Sbjct: 301 SHHQKIIIVDAQTHINAQNREIMSFIGGLDLCDGRYDTEQHSLFHTLNTESHCRDFYQTS 360

Query: 361 ISGAKLQKGGPREPWHDVHARVTGEAAWDILTNFEQRWTKQSDASLLVPTSILLKLMPPL 420
           ISGAKLQKGGPREPWHDVHA VTGEAAWDILTNFEQRWTKQSDASLLVPTSILLKLMP L
Sbjct: 361 ISGAKLQKGGPREPWHDVHACVTGEAAWDILTNFEQRWTKQSDASLLVPTSILLKLMPQL 420

Query: 421 ESNTNPQKDWNVQVFRSINHLSASQVFRNLTVERTIHEAYVEAIRRAERFIYIENQYFIG 480
           ESNTNPQKDWNVQVFRSI+HLSASQ+FRN+T+ERTIHEAYVEAIRRAERFIYIENQYFIG
Sbjct: 421 ESNTNPQKDWNVQVFRSIDHLSASQMFRNMTIERTIHEAYVEAIRRAERFIYIENQYFIG 480

Query: 481 GCHLWDKDQHCGCTNLIPIEIALKVANKIKAREKFAVYVVIPMWPEGPPESESVEDMLHW 540
           GCH WD+D+HCGCTNLIPIEIALKVANKIKARE+FAVY+VIPMWPEG PESESVEDMLHW
Sbjct: 481 GCHFWDRDRHCGCTNLIPIEIALKVANKIKARERFAVYIVIPMWPEGSPESESVEDMLHW 540

Query: 541 TRQTMTMMYRLIGEAIQETGEKAHPRDYLNFFCLANREEERKWDFVPPHSPQHATEYWSA 600
           TRQTMTMMYRLIGEAIQETGEKAHPRDYLNFFCLANREEERKWDF+PPHSPQHAT+YW+A
Sbjct: 541 TRQTMTMMYRLIGEAIQETGEKAHPRDYLNFFCLANREEERKWDFIPPHSPQHATQYWNA 600

Query: 601 QHHRRFMVYVHSKVMIVDDLYILIGSANVNQRSMDGERDTEIAIGCYQLENDGKELPNGR 660
           Q HRRFM+YVHSKVMIVDDLYILIGSANVNQRSMDGERDTEIAIGCYQ+ENDG+ELPNGR
Sbjct: 601 QQHRRFMIYVHSKVMIVDDLYILIGSANVNQRSMDGERDTEIAIGCYQIENDGEELPNGR 660

Query: 661 DISTFRLSLWYEHTQRFEEVFLNPENLQCVERVRSIADESWKIYSGEEVADMKGVHLVTY 720
           DIS FRLSLWYEHT+ FEEVFLNPE+L+CV+RVRSI D+SWKIYSGEEV DMKGVHLVTY
Sbjct: 661 DISRFRLSLWYEHTRGFEEVFLNPESLKCVQRVRSIGDKSWKIYSGEEVEDMKGVHLVTY 720

Query: 721 PVKVKQDGSIEDLEENGGHFPDTKCPIKGRRSMFLPPIFTT 762
           PVKVK+DG++EDLEENGGHFPDTKC IKGRRSM LPPIFTT
Sbjct: 721 PVKVKEDGTMEDLEENGGHFPDTKCAIKGRRSMMLPPIFTT 761

BLAST of CmoCh01G000700 vs. ExPASy TrEMBL
Match: A0A6J1D3G4 (Phospholipase D OS=Momordica charantia OX=3673 GN=LOC111016958 PE=3 SV=1)

HSP 1 Score: 1448.3 bits (3748), Expect = 0.0e+00
Identity = 673/761 (88.44%), Postives = 722/761 (94.88%), Query Frame = 0

Query: 1   MTVQGKHKFFHGTLEVTVFHATTYTPSSPLDCIFSGGKQSYVTIKINNIEVARTNHEYDR 60
           M ++GK  FFHGTLEVTVFHAT YTP SPLDC+FSGG+++YVT+KI+N EVA+T+HEYDR
Sbjct: 1   MEMEGKQMFFHGTLEVTVFHATAYTPPSPLDCLFSGGRRTYVTVKIDNKEVAQTSHEYDR 60

Query: 61  VWNQTFRVLCAHPLTSTITITMRTTRSVLGRFQIQAQQILKESSFINGFFPLLMENGKPS 120
           VWNQTF++LCA+P TST+TITM+T+RSVLG+F IQAQQILKE+SF+NGFFPL MENGKPS
Sbjct: 61  VWNQTFQILCAYPSTSTVTITMKTSRSVLGKFHIQAQQILKEASFMNGFFPLTMENGKPS 120

Query: 121 PELRLRFMLWFQPAVYELSWKKVLENGEYKGLRNATFPLRSNCHVTLYQDAHHVSTFQPP 180
           PELRLRFMLWF+PAVYELSWKK+L NGEYKGLRNATFPLRSNCHVTLYQDAHH+ TFQPP
Sbjct: 121 PELRLRFMLWFKPAVYELSWKKMLGNGEYKGLRNATFPLRSNCHVTLYQDAHHLPTFQPP 180

Query: 181 FHGSSAPRRLWEDVYKAIDNAKHLVYIAGWSFNPKMVLVRDPQTHIPYALGVKLGELLKQ 240
           FH SS+PRRLWEDVYKAIDNAKHL+YIAGWSFNPKM+LVRD QT IPYALGVKLGELLKQ
Sbjct: 181 FHSSSSPRRLWEDVYKAIDNAKHLIYIAGWSFNPKMLLVRDSQTDIPYALGVKLGELLKQ 240

Query: 241 KAEEGVAVRLMIWDDETSLPIIKNAGIMKTHDEDARAYFSNSKVVCRLCPKLHPMSPPIF 300
           KAEEGVAVR+MIWDD TSLPIIKNAGIMKT DEDA AYFS+SKV+CRLCPKLHPM+PPIF
Sbjct: 241 KAEEGVAVRIMIWDDATSLPIIKNAGIMKTRDEDAWAYFSHSKVICRLCPKLHPMAPPIF 300

Query: 301 SHHQKTITVDAQTHINARNREIMSFIGGLDLCDGRYDTEQHSLFHTLNTESHCCDFYQTS 360
           SHHQKTITVDAQ HINARNREIMSFIGGLDLCDGRYDTEQHSLFHTLN ESHC DFYQTS
Sbjct: 301 SHHQKTITVDAQAHINARNREIMSFIGGLDLCDGRYDTEQHSLFHTLNMESHCGDFYQTS 360

Query: 361 ISGAKLQKGGPREPWHDVHARVTGEAAWDILTNFEQRWTKQSDASLLVPTSILLKLMPPL 420
           ISGAKLQKGGPREPWHDVHA VTGEAAWDILTNFEQRWTKQSDASLLVPTSILL LMP L
Sbjct: 361 ISGAKLQKGGPREPWHDVHACVTGEAAWDILTNFEQRWTKQSDASLLVPTSILLNLMPQL 420

Query: 421 ESNTNPQKDWNVQVFRSINHLSASQVFRNLTVERTIHEAYVEAIRRAERFIYIENQYFIG 480
           E+NTNPQ DWNVQVFRSI+HLSASQVFRNLTVERTIHEAYVEAIRRAERFIYIENQYFIG
Sbjct: 421 ETNTNPQNDWNVQVFRSIDHLSASQVFRNLTVERTIHEAYVEAIRRAERFIYIENQYFIG 480

Query: 481 GCHLWDKDQHCGCTNLIPIEIALKVANKIKAREKFAVYVVIPMWPEGPPESESVEDMLHW 540
           GCHLWDKDQHCGCTNLIPIEIALKVANKIKARE+FAVY+VIPMWPEGPPESESV +MLHW
Sbjct: 481 GCHLWDKDQHCGCTNLIPIEIALKVANKIKARERFAVYIVIPMWPEGPPESESVAEMLHW 540

Query: 541 TRQTMTMMYRLIGEAIQETGEKAHPRDYLNFFCLANREEERKWDFVPPHSPQHATEYWSA 600
           TRQTM MMY+LIGEAIQETGEKAHPRDYLNFFCLANREEE +WDFVPPHSP+ AT+YW+A
Sbjct: 541 TRQTMKMMYKLIGEAIQETGEKAHPRDYLNFFCLANREEEGEWDFVPPHSPERATQYWNA 600

Query: 601 QHHRRFMVYVHSKVMIVDDLYILIGSANVNQRSMDGERDTEIAIGCYQLENDGKELPNGR 660
           Q HRRFMVYVHSKVMIVDDLYILIGSANVNQRSMDG RDTEIAIGC+Q E D +E+PNGR
Sbjct: 601 QQHRRFMVYVHSKVMIVDDLYILIGSANVNQRSMDGGRDTEIAIGCFQQEKDAEEMPNGR 660

Query: 661 DISTFRLSLWYEHTQRFEEVFLNPENLQCVERVRSIADESWKIYSGEEVADMKGVHLVTY 720
           DISTFRLSLWYEHT+RFEE+FLNPE L CV+RVRSIADESW+IYSGEE ADM+GVHLV Y
Sbjct: 661 DISTFRLSLWYEHTRRFEELFLNPETLDCVQRVRSIADESWRIYSGEEAADMEGVHLVAY 720

Query: 721 PVKVKQDGSIEDLEENGGHFPDTKCPIKGRRSMFLPPIFTT 762
           PVKV Q+GS+EDLEENGGHFPDTKCPIKGRRSM LPPIFTT
Sbjct: 721 PVKVNQEGSVEDLEENGGHFPDTKCPIKGRRSMLLPPIFTT 761

BLAST of CmoCh01G000700 vs. NCBI nr
Match: XP_022948424.1 (phospholipase D alpha 4 [Cucurbita moschata])

HSP 1 Score: 1603.6 bits (4151), Expect = 0.0e+00
Identity = 761/761 (100.00%), Postives = 761/761 (100.00%), Query Frame = 0

Query: 1   MTVQGKHKFFHGTLEVTVFHATTYTPSSPLDCIFSGGKQSYVTIKINNIEVARTNHEYDR 60
           MTVQGKHKFFHGTLEVTVFHATTYTPSSPLDCIFSGGKQSYVTIKINNIEVARTNHEYDR
Sbjct: 62  MTVQGKHKFFHGTLEVTVFHATTYTPSSPLDCIFSGGKQSYVTIKINNIEVARTNHEYDR 121

Query: 61  VWNQTFRVLCAHPLTSTITITMRTTRSVLGRFQIQAQQILKESSFINGFFPLLMENGKPS 120
           VWNQTFRVLCAHPLTSTITITMRTTRSVLGRFQIQAQQILKESSFINGFFPLLMENGKPS
Sbjct: 122 VWNQTFRVLCAHPLTSTITITMRTTRSVLGRFQIQAQQILKESSFINGFFPLLMENGKPS 181

Query: 121 PELRLRFMLWFQPAVYELSWKKVLENGEYKGLRNATFPLRSNCHVTLYQDAHHVSTFQPP 180
           PELRLRFMLWFQPAVYELSWKKVLENGEYKGLRNATFPLRSNCHVTLYQDAHHVSTFQPP
Sbjct: 182 PELRLRFMLWFQPAVYELSWKKVLENGEYKGLRNATFPLRSNCHVTLYQDAHHVSTFQPP 241

Query: 181 FHGSSAPRRLWEDVYKAIDNAKHLVYIAGWSFNPKMVLVRDPQTHIPYALGVKLGELLKQ 240
           FHGSSAPRRLWEDVYKAIDNAKHLVYIAGWSFNPKMVLVRDPQTHIPYALGVKLGELLKQ
Sbjct: 242 FHGSSAPRRLWEDVYKAIDNAKHLVYIAGWSFNPKMVLVRDPQTHIPYALGVKLGELLKQ 301

Query: 241 KAEEGVAVRLMIWDDETSLPIIKNAGIMKTHDEDARAYFSNSKVVCRLCPKLHPMSPPIF 300
           KAEEGVAVRLMIWDDETSLPIIKNAGIMKTHDEDARAYFSNSKVVCRLCPKLHPMSPPIF
Sbjct: 302 KAEEGVAVRLMIWDDETSLPIIKNAGIMKTHDEDARAYFSNSKVVCRLCPKLHPMSPPIF 361

Query: 301 SHHQKTITVDAQTHINARNREIMSFIGGLDLCDGRYDTEQHSLFHTLNTESHCCDFYQTS 360
           SHHQKTITVDAQTHINARNREIMSFIGGLDLCDGRYDTEQHSLFHTLNTESHCCDFYQTS
Sbjct: 362 SHHQKTITVDAQTHINARNREIMSFIGGLDLCDGRYDTEQHSLFHTLNTESHCCDFYQTS 421

Query: 361 ISGAKLQKGGPREPWHDVHARVTGEAAWDILTNFEQRWTKQSDASLLVPTSILLKLMPPL 420
           ISGAKLQKGGPREPWHDVHARVTGEAAWDILTNFEQRWTKQSDASLLVPTSILLKLMPPL
Sbjct: 422 ISGAKLQKGGPREPWHDVHARVTGEAAWDILTNFEQRWTKQSDASLLVPTSILLKLMPPL 481

Query: 421 ESNTNPQKDWNVQVFRSINHLSASQVFRNLTVERTIHEAYVEAIRRAERFIYIENQYFIG 480
           ESNTNPQKDWNVQVFRSINHLSASQVFRNLTVERTIHEAYVEAIRRAERFIYIENQYFIG
Sbjct: 482 ESNTNPQKDWNVQVFRSINHLSASQVFRNLTVERTIHEAYVEAIRRAERFIYIENQYFIG 541

Query: 481 GCHLWDKDQHCGCTNLIPIEIALKVANKIKAREKFAVYVVIPMWPEGPPESESVEDMLHW 540
           GCHLWDKDQHCGCTNLIPIEIALKVANKIKAREKFAVYVVIPMWPEGPPESESVEDMLHW
Sbjct: 542 GCHLWDKDQHCGCTNLIPIEIALKVANKIKAREKFAVYVVIPMWPEGPPESESVEDMLHW 601

Query: 541 TRQTMTMMYRLIGEAIQETGEKAHPRDYLNFFCLANREEERKWDFVPPHSPQHATEYWSA 600
           TRQTMTMMYRLIGEAIQETGEKAHPRDYLNFFCLANREEERKWDFVPPHSPQHATEYWSA
Sbjct: 602 TRQTMTMMYRLIGEAIQETGEKAHPRDYLNFFCLANREEERKWDFVPPHSPQHATEYWSA 661

Query: 601 QHHRRFMVYVHSKVMIVDDLYILIGSANVNQRSMDGERDTEIAIGCYQLENDGKELPNGR 660
           QHHRRFMVYVHSKVMIVDDLYILIGSANVNQRSMDGERDTEIAIGCYQLENDGKELPNGR
Sbjct: 662 QHHRRFMVYVHSKVMIVDDLYILIGSANVNQRSMDGERDTEIAIGCYQLENDGKELPNGR 721

Query: 661 DISTFRLSLWYEHTQRFEEVFLNPENLQCVERVRSIADESWKIYSGEEVADMKGVHLVTY 720
           DISTFRLSLWYEHTQRFEEVFLNPENLQCVERVRSIADESWKIYSGEEVADMKGVHLVTY
Sbjct: 722 DISTFRLSLWYEHTQRFEEVFLNPENLQCVERVRSIADESWKIYSGEEVADMKGVHLVTY 781

Query: 721 PVKVKQDGSIEDLEENGGHFPDTKCPIKGRRSMFLPPIFTT 762
           PVKVKQDGSIEDLEENGGHFPDTKCPIKGRRSMFLPPIFTT
Sbjct: 782 PVKVKQDGSIEDLEENGGHFPDTKCPIKGRRSMFLPPIFTT 822

BLAST of CmoCh01G000700 vs. NCBI nr
Match: KAG7036437.1 (Phospholipase D alpha 4 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1601.6 bits (4146), Expect = 0.0e+00
Identity = 760/761 (99.87%), Postives = 761/761 (100.00%), Query Frame = 0

Query: 1   MTVQGKHKFFHGTLEVTVFHATTYTPSSPLDCIFSGGKQSYVTIKINNIEVARTNHEYDR 60
           MTVQGKHKFFHGTLEVTVFHATTYTPSSPLDCIFSGGKQSYVTIKINNIEVARTNHEYDR
Sbjct: 1   MTVQGKHKFFHGTLEVTVFHATTYTPSSPLDCIFSGGKQSYVTIKINNIEVARTNHEYDR 60

Query: 61  VWNQTFRVLCAHPLTSTITITMRTTRSVLGRFQIQAQQILKESSFINGFFPLLMENGKPS 120
           VWNQTFRVLCAHPLTSTITITMRTTRSVLGRFQIQAQQILKESSFINGFFPLLMENGKPS
Sbjct: 61  VWNQTFRVLCAHPLTSTITITMRTTRSVLGRFQIQAQQILKESSFINGFFPLLMENGKPS 120

Query: 121 PELRLRFMLWFQPAVYELSWKKVLENGEYKGLRNATFPLRSNCHVTLYQDAHHVSTFQPP 180
           PELRLRFMLWFQPAVYELSWKKVLENGEYKGLRNATFPLRSNCHVTLYQDAHHVSTFQPP
Sbjct: 121 PELRLRFMLWFQPAVYELSWKKVLENGEYKGLRNATFPLRSNCHVTLYQDAHHVSTFQPP 180

Query: 181 FHGSSAPRRLWEDVYKAIDNAKHLVYIAGWSFNPKMVLVRDPQTHIPYALGVKLGELLKQ 240
           FHGSSAPRRLWEDVYKAIDNAKHLVYIAGWSFNPKMVLVRDPQTHIPYALGVKLGELLKQ
Sbjct: 181 FHGSSAPRRLWEDVYKAIDNAKHLVYIAGWSFNPKMVLVRDPQTHIPYALGVKLGELLKQ 240

Query: 241 KAEEGVAVRLMIWDDETSLPIIKNAGIMKTHDEDARAYFSNSKVVCRLCPKLHPMSPPIF 300
           KAEEGVAVRLMIWDDETSLPIIKNAGIMKTHDEDARAYFSNSKVVCRLCPKLHPMSPPIF
Sbjct: 241 KAEEGVAVRLMIWDDETSLPIIKNAGIMKTHDEDARAYFSNSKVVCRLCPKLHPMSPPIF 300

Query: 301 SHHQKTITVDAQTHINARNREIMSFIGGLDLCDGRYDTEQHSLFHTLNTESHCCDFYQTS 360
           SHHQKTITVDAQTHINARNREIMSFIGGLDLCDGRYDTEQHSLFHTLNTESHCCDFYQTS
Sbjct: 301 SHHQKTITVDAQTHINARNREIMSFIGGLDLCDGRYDTEQHSLFHTLNTESHCCDFYQTS 360

Query: 361 ISGAKLQKGGPREPWHDVHARVTGEAAWDILTNFEQRWTKQSDASLLVPTSILLKLMPPL 420
           ISGAKLQKGGPREPWHDVHARVTGEAAWDILTNFEQRWTKQSDASLLVPTSILLKLMPPL
Sbjct: 361 ISGAKLQKGGPREPWHDVHARVTGEAAWDILTNFEQRWTKQSDASLLVPTSILLKLMPPL 420

Query: 421 ESNTNPQKDWNVQVFRSINHLSASQVFRNLTVERTIHEAYVEAIRRAERFIYIENQYFIG 480
           ESNTNPQKDWNVQVFRSI+HLSASQVFRNLTVERTIHEAYVEAIRRAERFIYIENQYFIG
Sbjct: 421 ESNTNPQKDWNVQVFRSIDHLSASQVFRNLTVERTIHEAYVEAIRRAERFIYIENQYFIG 480

Query: 481 GCHLWDKDQHCGCTNLIPIEIALKVANKIKAREKFAVYVVIPMWPEGPPESESVEDMLHW 540
           GCHLWDKDQHCGCTNLIPIEIALKVANKIKAREKFAVYVVIPMWPEGPPESESVEDMLHW
Sbjct: 481 GCHLWDKDQHCGCTNLIPIEIALKVANKIKAREKFAVYVVIPMWPEGPPESESVEDMLHW 540

Query: 541 TRQTMTMMYRLIGEAIQETGEKAHPRDYLNFFCLANREEERKWDFVPPHSPQHATEYWSA 600
           TRQTMTMMYRLIGEAIQETGEKAHPRDYLNFFCLANREEERKWDFVPPHSPQHATEYWSA
Sbjct: 541 TRQTMTMMYRLIGEAIQETGEKAHPRDYLNFFCLANREEERKWDFVPPHSPQHATEYWSA 600

Query: 601 QHHRRFMVYVHSKVMIVDDLYILIGSANVNQRSMDGERDTEIAIGCYQLENDGKELPNGR 660
           QHHRRFMVYVHSKVMIVDDLYILIGSANVNQRSMDGERDTEIAIGCYQLENDGKELPNGR
Sbjct: 601 QHHRRFMVYVHSKVMIVDDLYILIGSANVNQRSMDGERDTEIAIGCYQLENDGKELPNGR 660

Query: 661 DISTFRLSLWYEHTQRFEEVFLNPENLQCVERVRSIADESWKIYSGEEVADMKGVHLVTY 720
           DISTFRLSLWYEHTQRFEEVFLNPENLQCVERVRSIADESWKIYSGEEVADMKGVHLVTY
Sbjct: 661 DISTFRLSLWYEHTQRFEEVFLNPENLQCVERVRSIADESWKIYSGEEVADMKGVHLVTY 720

Query: 721 PVKVKQDGSIEDLEENGGHFPDTKCPIKGRRSMFLPPIFTT 762
           PVKVKQDGSIEDLEENGGHFPDTKCPIKGRRSMFLPPIFTT
Sbjct: 721 PVKVKQDGSIEDLEENGGHFPDTKCPIKGRRSMFLPPIFTT 761

BLAST of CmoCh01G000700 vs. NCBI nr
Match: KAG6606722.1 (Phospholipase D alpha 4, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1600.1 bits (4142), Expect = 0.0e+00
Identity = 758/761 (99.61%), Postives = 761/761 (100.00%), Query Frame = 0

Query: 1   MTVQGKHKFFHGTLEVTVFHATTYTPSSPLDCIFSGGKQSYVTIKINNIEVARTNHEYDR 60
           MT+QGKHKFFHGTLEVTVFHATTYTPSSPLDCIFSGGKQSYVTIKINNIEVARTNHEYDR
Sbjct: 61  MTLQGKHKFFHGTLEVTVFHATTYTPSSPLDCIFSGGKQSYVTIKINNIEVARTNHEYDR 120

Query: 61  VWNQTFRVLCAHPLTSTITITMRTTRSVLGRFQIQAQQILKESSFINGFFPLLMENGKPS 120
           VWNQTFRVLCAHPLTSTITITMRTTRSVLGRFQIQAQQILKESSFINGFFPLLMENGKPS
Sbjct: 121 VWNQTFRVLCAHPLTSTITITMRTTRSVLGRFQIQAQQILKESSFINGFFPLLMENGKPS 180

Query: 121 PELRLRFMLWFQPAVYELSWKKVLENGEYKGLRNATFPLRSNCHVTLYQDAHHVSTFQPP 180
           PELRLRFMLWFQPAVYELSWKKVLENGEYKGLRNATFPLRSNCHVTLYQDAHHVSTFQPP
Sbjct: 181 PELRLRFMLWFQPAVYELSWKKVLENGEYKGLRNATFPLRSNCHVTLYQDAHHVSTFQPP 240

Query: 181 FHGSSAPRRLWEDVYKAIDNAKHLVYIAGWSFNPKMVLVRDPQTHIPYALGVKLGELLKQ 240
           FHGSSAPRRLWEDVYKAIDNAKHLVYIAGWSFNPKMVLVRDPQTHIPYALGVKLGELLKQ
Sbjct: 241 FHGSSAPRRLWEDVYKAIDNAKHLVYIAGWSFNPKMVLVRDPQTHIPYALGVKLGELLKQ 300

Query: 241 KAEEGVAVRLMIWDDETSLPIIKNAGIMKTHDEDARAYFSNSKVVCRLCPKLHPMSPPIF 300
           KAEEGVAVRLMIWDDETSLPIIKNAGIMKTHDEDARAYFSNSKVVCRLCPKLHPMSPPIF
Sbjct: 301 KAEEGVAVRLMIWDDETSLPIIKNAGIMKTHDEDARAYFSNSKVVCRLCPKLHPMSPPIF 360

Query: 301 SHHQKTITVDAQTHINARNREIMSFIGGLDLCDGRYDTEQHSLFHTLNTESHCCDFYQTS 360
           SHHQKTITVDAQTHINARNREIMSFIGGLDLCDGRYDTEQHSLFHTLNTESHCCDFYQTS
Sbjct: 361 SHHQKTITVDAQTHINARNREIMSFIGGLDLCDGRYDTEQHSLFHTLNTESHCCDFYQTS 420

Query: 361 ISGAKLQKGGPREPWHDVHARVTGEAAWDILTNFEQRWTKQSDASLLVPTSILLKLMPPL 420
           ISGAKLQKGGPREPWHDVHARVTGEAAWDILTNFEQRWTKQSDASLLVPTSILLKLMPPL
Sbjct: 421 ISGAKLQKGGPREPWHDVHARVTGEAAWDILTNFEQRWTKQSDASLLVPTSILLKLMPPL 480

Query: 421 ESNTNPQKDWNVQVFRSINHLSASQVFRNLTVERTIHEAYVEAIRRAERFIYIENQYFIG 480
           ESNTNPQKDWNVQVFRSI+HLSASQVFRNLTVERTIHEAYVEAIRRAERFIYIENQYFIG
Sbjct: 481 ESNTNPQKDWNVQVFRSIDHLSASQVFRNLTVERTIHEAYVEAIRRAERFIYIENQYFIG 540

Query: 481 GCHLWDKDQHCGCTNLIPIEIALKVANKIKAREKFAVYVVIPMWPEGPPESESVEDMLHW 540
           GCHLWDKDQHCGCTNLIPIEIALKVANKIKAREKFAVYVVIPMWPEGPPESESVEDMLHW
Sbjct: 541 GCHLWDKDQHCGCTNLIPIEIALKVANKIKAREKFAVYVVIPMWPEGPPESESVEDMLHW 600

Query: 541 TRQTMTMMYRLIGEAIQETGEKAHPRDYLNFFCLANREEERKWDFVPPHSPQHATEYWSA 600
           TRQTMTMMYRLIGEAIQETGEKAHPRDYLNFFCLANREEERKWDFVPPHSPQHATEYWSA
Sbjct: 601 TRQTMTMMYRLIGEAIQETGEKAHPRDYLNFFCLANREEERKWDFVPPHSPQHATEYWSA 660

Query: 601 QHHRRFMVYVHSKVMIVDDLYILIGSANVNQRSMDGERDTEIAIGCYQLENDGKELPNGR 660
           QHHRRFMVYVHSKVMIVDDLYILIGSANVNQRSMDGERDTEIAIGCYQLENDGKELPNGR
Sbjct: 661 QHHRRFMVYVHSKVMIVDDLYILIGSANVNQRSMDGERDTEIAIGCYQLENDGKELPNGR 720

Query: 661 DISTFRLSLWYEHTQRFEEVFLNPENLQCVERVRSIADESWKIYSGEEVADMKGVHLVTY 720
           DISTFRLSLWYEHTQRFEEVFLNPENLQCVER+RSIADESWKIYSGEEVADMKGVHLVTY
Sbjct: 721 DISTFRLSLWYEHTQRFEEVFLNPENLQCVERIRSIADESWKIYSGEEVADMKGVHLVTY 780

Query: 721 PVKVKQDGSIEDLEENGGHFPDTKCPIKGRRSMFLPPIFTT 762
           PVKVKQDGSIEDLEENGGHFPDTKCPIKGRRSMFLPPIFTT
Sbjct: 781 PVKVKQDGSIEDLEENGGHFPDTKCPIKGRRSMFLPPIFTT 821

BLAST of CmoCh01G000700 vs. NCBI nr
Match: XP_023525777.1 (phospholipase D alpha 4 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1587.8 bits (4110), Expect = 0.0e+00
Identity = 753/761 (98.95%), Postives = 757/761 (99.47%), Query Frame = 0

Query: 1   MTVQGKHKFFHGTLEVTVFHATTYTPSSPLDCIFSGGKQSYVTIKINNIEVARTNHEYDR 60
           MTV GKHKFFHGTLEVTVFHATTYTPSSPLDCIFSGGKQSYVTIKINNIEVARTNHEYDR
Sbjct: 61  MTVHGKHKFFHGTLEVTVFHATTYTPSSPLDCIFSGGKQSYVTIKINNIEVARTNHEYDR 120

Query: 61  VWNQTFRVLCAHPLTSTITITMRTTRSVLGRFQIQAQQILKESSFINGFFPLLMENGKPS 120
           VWNQTFRVLCAHPLTSTITITMRT+RSVLGRFQIQAQQILKESSFINGFFPLLMENGKPS
Sbjct: 121 VWNQTFRVLCAHPLTSTITITMRTSRSVLGRFQIQAQQILKESSFINGFFPLLMENGKPS 180

Query: 121 PELRLRFMLWFQPAVYELSWKKVLENGEYKGLRNATFPLRSNCHVTLYQDAHHVSTFQPP 180
           PELRLRFMLWFQPAVYELSWKKVLENGEYKGLRNATFPLRSNCHVTLYQDAHHVSTFQPP
Sbjct: 181 PELRLRFMLWFQPAVYELSWKKVLENGEYKGLRNATFPLRSNCHVTLYQDAHHVSTFQPP 240

Query: 181 FHGSSAPRRLWEDVYKAIDNAKHLVYIAGWSFNPKMVLVRDPQTHIPYALGVKLGELLKQ 240
           FHGSSAPRRLWEDVYKAIDNAKHLVYIAGWSFNPKMVLVRDPQT IPYALGVKLGELLKQ
Sbjct: 241 FHGSSAPRRLWEDVYKAIDNAKHLVYIAGWSFNPKMVLVRDPQTDIPYALGVKLGELLKQ 300

Query: 241 KAEEGVAVRLMIWDDETSLPIIKNAGIMKTHDEDARAYFSNSKVVCRLCPKLHPMSPPIF 300
           KAEEGVAVRLMIWDDETSLP+IKNAGIMKTHDEDARAYFSNSKVVCRLCPKLHPMSPPIF
Sbjct: 301 KAEEGVAVRLMIWDDETSLPVIKNAGIMKTHDEDARAYFSNSKVVCRLCPKLHPMSPPIF 360

Query: 301 SHHQKTITVDAQTHINARNREIMSFIGGLDLCDGRYDTEQHSLFHTLNTESHCCDFYQTS 360
           SHHQKTITVDAQTHINARNREIMSFIGGLDLCDGRYDTEQHSLFHTLNTESHCCDFYQTS
Sbjct: 361 SHHQKTITVDAQTHINARNREIMSFIGGLDLCDGRYDTEQHSLFHTLNTESHCCDFYQTS 420

Query: 361 ISGAKLQKGGPREPWHDVHARVTGEAAWDILTNFEQRWTKQSDASLLVPTSILLKLMPPL 420
           ISGAKLQKGGPREPWHDVHARVTGEAAWDILTNFEQRWTKQSDASLLVPTSILLKLMPPL
Sbjct: 421 ISGAKLQKGGPREPWHDVHARVTGEAAWDILTNFEQRWTKQSDASLLVPTSILLKLMPPL 480

Query: 421 ESNTNPQKDWNVQVFRSINHLSASQVFRNLTVERTIHEAYVEAIRRAERFIYIENQYFIG 480
           ESNTNPQKDWNVQVFRSI+HLSASQVFRNLTVERTIHEAYVEAIRRAERFIYIENQYFIG
Sbjct: 481 ESNTNPQKDWNVQVFRSIDHLSASQVFRNLTVERTIHEAYVEAIRRAERFIYIENQYFIG 540

Query: 481 GCHLWDKDQHCGCTNLIPIEIALKVANKIKAREKFAVYVVIPMWPEGPPESESVEDMLHW 540
           GCHLWDKDQHCGCTNLIPIEIALKVA KI+AREKFAVYVVIPMWPEGPPESESVEDMLHW
Sbjct: 541 GCHLWDKDQHCGCTNLIPIEIALKVATKIRAREKFAVYVVIPMWPEGPPESESVEDMLHW 600

Query: 541 TRQTMTMMYRLIGEAIQETGEKAHPRDYLNFFCLANREEERKWDFVPPHSPQHATEYWSA 600
           TRQTMTMMYRLIGEAIQETGEKAHPRDYLNFFCLANREEERKWDFVPPHSPQHATEYWSA
Sbjct: 601 TRQTMTMMYRLIGEAIQETGEKAHPRDYLNFFCLANREEERKWDFVPPHSPQHATEYWSA 660

Query: 601 QHHRRFMVYVHSKVMIVDDLYILIGSANVNQRSMDGERDTEIAIGCYQLENDGKELPNGR 660
           Q HRRFMVYVHSKVMIVDDLYILIGSANVNQRSMDGERDTEIAIGCYQLENDGKELPNGR
Sbjct: 661 QQHRRFMVYVHSKVMIVDDLYILIGSANVNQRSMDGERDTEIAIGCYQLENDGKELPNGR 720

Query: 661 DISTFRLSLWYEHTQRFEEVFLNPENLQCVERVRSIADESWKIYSGEEVADMKGVHLVTY 720
           DISTFRLSLWYEHTQRFEEVFLNPENLQCVERVRSIADESWKIYSGEEVADMKGVHLVTY
Sbjct: 721 DISTFRLSLWYEHTQRFEEVFLNPENLQCVERVRSIADESWKIYSGEEVADMKGVHLVTY 780

Query: 721 PVKVKQDGSIEDLEENGGHFPDTKCPIKGRRSMFLPPIFTT 762
           PVKVKQDGSIEDLEENGGHFPDTKCPIKGRRSMFLPPIFTT
Sbjct: 781 PVKVKQDGSIEDLEENGGHFPDTKCPIKGRRSMFLPPIFTT 821

BLAST of CmoCh01G000700 vs. NCBI nr
Match: XP_022997750.1 (phospholipase D alpha 4 [Cucurbita maxima])

HSP 1 Score: 1586.6 bits (4107), Expect = 0.0e+00
Identity = 752/761 (98.82%), Postives = 757/761 (99.47%), Query Frame = 0

Query: 1   MTVQGKHKFFHGTLEVTVFHATTYTPSSPLDCIFSGGKQSYVTIKINNIEVARTNHEYDR 60
           MTVQG+H FFHGTLEVTVFHATTYTPSSPLDCIFSGGKQSYVTIKINNIEVARTNHEYDR
Sbjct: 1   MTVQGEHTFFHGTLEVTVFHATTYTPSSPLDCIFSGGKQSYVTIKINNIEVARTNHEYDR 60

Query: 61  VWNQTFRVLCAHPLTSTITITMRTTRSVLGRFQIQAQQILKESSFINGFFPLLMENGKPS 120
           VWNQTFRVLCAHPLTSTITITMRTTRSVLGRFQIQAQQILKESSFINGFFPLLMENGKPS
Sbjct: 61  VWNQTFRVLCAHPLTSTITITMRTTRSVLGRFQIQAQQILKESSFINGFFPLLMENGKPS 120

Query: 121 PELRLRFMLWFQPAVYELSWKKVLENGEYKGLRNATFPLRSNCHVTLYQDAHHVSTFQPP 180
           PELRLRFMLWFQPAVYELSWKKVLENGEYKGLRN TFPLRSNCHVTLYQDAHHVSTFQPP
Sbjct: 121 PELRLRFMLWFQPAVYELSWKKVLENGEYKGLRNVTFPLRSNCHVTLYQDAHHVSTFQPP 180

Query: 181 FHGSSAPRRLWEDVYKAIDNAKHLVYIAGWSFNPKMVLVRDPQTHIPYALGVKLGELLKQ 240
           FHGSSAPRRLWEDVYKAIDNAKHLVYIAGWSFNPKMVLVRDPQT IPYALGVKLGELLKQ
Sbjct: 181 FHGSSAPRRLWEDVYKAIDNAKHLVYIAGWSFNPKMVLVRDPQTDIPYALGVKLGELLKQ 240

Query: 241 KAEEGVAVRLMIWDDETSLPIIKNAGIMKTHDEDARAYFSNSKVVCRLCPKLHPMSPPIF 300
           KAEEGVAVRLMIWDDETSLP+IKNAGIMKTHDE+ARAYFSNSKVVCRLCPKLHPMSPPIF
Sbjct: 241 KAEEGVAVRLMIWDDETSLPVIKNAGIMKTHDENARAYFSNSKVVCRLCPKLHPMSPPIF 300

Query: 301 SHHQKTITVDAQTHINARNREIMSFIGGLDLCDGRYDTEQHSLFHTLNTESHCCDFYQTS 360
           SHHQKTITVDAQTHINARNREIMSFIGGLDLCDGRYDTEQHSLFHTLNTESHCCDFYQTS
Sbjct: 301 SHHQKTITVDAQTHINARNREIMSFIGGLDLCDGRYDTEQHSLFHTLNTESHCCDFYQTS 360

Query: 361 ISGAKLQKGGPREPWHDVHARVTGEAAWDILTNFEQRWTKQSDASLLVPTSILLKLMPPL 420
           ISGAKLQKGGPREPWHDVHARVTGEAAWDILTNFEQRWTKQSDASLLVPTSILLKLMPPL
Sbjct: 361 ISGAKLQKGGPREPWHDVHARVTGEAAWDILTNFEQRWTKQSDASLLVPTSILLKLMPPL 420

Query: 421 ESNTNPQKDWNVQVFRSINHLSASQVFRNLTVERTIHEAYVEAIRRAERFIYIENQYFIG 480
           ESNTNPQKDWNVQVFRSI+HLSASQVFRNLTVERTIHEAYVEAIRRAERFIYIENQYFIG
Sbjct: 421 ESNTNPQKDWNVQVFRSIDHLSASQVFRNLTVERTIHEAYVEAIRRAERFIYIENQYFIG 480

Query: 481 GCHLWDKDQHCGCTNLIPIEIALKVANKIKAREKFAVYVVIPMWPEGPPESESVEDMLHW 540
           GCHLWDKDQHCGCTNLIPIEIALKVANKIKAREKFAVYVVIPMWPEGPPESESVEDMLHW
Sbjct: 481 GCHLWDKDQHCGCTNLIPIEIALKVANKIKAREKFAVYVVIPMWPEGPPESESVEDMLHW 540

Query: 541 TRQTMTMMYRLIGEAIQETGEKAHPRDYLNFFCLANREEERKWDFVPPHSPQHATEYWSA 600
           TRQTMTMMYRLIGEAIQETGEKAHPRDYLNFFCLANREEERKWDFVPPHSPQHATEYWSA
Sbjct: 541 TRQTMTMMYRLIGEAIQETGEKAHPRDYLNFFCLANREEERKWDFVPPHSPQHATEYWSA 600

Query: 601 QHHRRFMVYVHSKVMIVDDLYILIGSANVNQRSMDGERDTEIAIGCYQLENDGKELPNGR 660
           QH RRFMVYVHSKVMIVDDLY+LIGSANVNQRSMDGERDTEIAIGCYQLENDGKELPNGR
Sbjct: 601 QHRRRFMVYVHSKVMIVDDLYLLIGSANVNQRSMDGERDTEIAIGCYQLENDGKELPNGR 660

Query: 661 DISTFRLSLWYEHTQRFEEVFLNPENLQCVERVRSIADESWKIYSGEEVADMKGVHLVTY 720
           DISTFRLSLWYEHTQRFEEVFLNPENLQCVERVRSIADESWKIYSGEEVADMKGVHLVTY
Sbjct: 661 DISTFRLSLWYEHTQRFEEVFLNPENLQCVERVRSIADESWKIYSGEEVADMKGVHLVTY 720

Query: 721 PVKVKQDGSIEDLEENGGHFPDTKCPIKGRRSMFLPPIFTT 762
           PVKVKQDGSIEDLEENGGHFPDTKCPIKGRRSMFLPPIFTT
Sbjct: 721 PVKVKQDGSIEDLEENGGHFPDTKCPIKGRRSMFLPPIFTT 761

BLAST of CmoCh01G000700 vs. TAIR 10
Match: AT1G55180.1 (phospholipase D alpha 4 )

HSP 1 Score: 977.2 bits (2525), Expect = 7.3e-285
Identity = 468/779 (60.08%), Postives = 599/779 (76.89%), Query Frame = 0

Query: 1   MTVQGKHKFFHGTLEVTVFHATTYTPSSPLDCIFSGGKQSYVTIKINNIEVARTNHEYDR 60
           M ++ + K+FHGTLE+T+F AT ++P  P +CI +  K +YVTIKIN  +VA+T+ EYDR
Sbjct: 1   MELEEQKKYFHGTLEITIFDATPFSPPFPFNCICTKPKAAYVTIKINKKKVAKTSSEYDR 60

Query: 61  VWNQTFRVLCAHPLT-STITITMRTTRSVLGRFQIQAQQIL-KESSFINGFFPLLMENGK 120
           +WNQTF++LCAHP+T +TITIT++T  SVLGRF+I A+QIL   S+ INGFFPL+ +NG 
Sbjct: 61  IWNQTFQILCAHPVTDTTITITLKTRCSVLGRFRISAEQILTSNSAVINGFFPLIADNGS 120

Query: 121 PSPELRLRFMLWFQPAVYELSWKKVLENGEYKGLRNATFPLRSNCHVTLYQDAHHVSTFQ 180
               L+L+ ++WF+PA  E  W + LE   ++G+RNA+FP RSNC V LYQDAHH +TF 
Sbjct: 121 TKRNLKLKCLMWFRPAYLEPGWCRALEEASFQGIRNASFPQRSNCRVVLYQDAHHKATFD 180

Query: 181 P-----PFHGSSAPRRLWEDVYKAIDNAKHLVYIAGWSFNPKMVLVRDPQTHIPYALGVK 240
           P     PF+     R LWEDVYKAI++A+HLVYIAGW+ NP +VLVRD +T IP+A+GV 
Sbjct: 181 PRVDDVPFNA----RNLWEDVYKAIESARHLVYIAGWALNPNLVLVRDNETEIPHAVGVT 240

Query: 241 LGELLKQKAEEGVAVRLMIWDDETSLPIIKNAGIMKTHDEDARAYFSNSKVVCRLCPKLH 300
           +GELLK+K+EEGVAVR+M+W+DETSLP+IKN G+M+T+ E A AYF N+ VVCRLCP+LH
Sbjct: 241 VGELLKRKSEEGVAVRVMLWNDETSLPMIKNKGVMRTNVERALAYFRNTNVVCRLCPRLH 300

Query: 301 PMSPPIFSHHQKTITVDAQ-THINARNREIMSFIGGLDLCDGRYDTEQHSLFHTLNTESH 360
              P  F+HHQKTIT+D + T+ + + REIMSF+GG DLCDGRYDTE+HSLF TL TE+ 
Sbjct: 301 KKLPTAFAHHQKTITLDTRVTNSSTKEREIMSFLGGFDLCDGRYDTEEHSLFRTLGTEA- 360

Query: 361 CCDFYQTSISGAKLQKGGPREPWHDVHARVTGEAAWDILTNFEQRWTKQSDASLLVPTS- 420
             DFYQTS++GAKL +GGPREPWHD H  V G AAWD+L NFEQRWTKQ + S+LV TS 
Sbjct: 361 --DFYQTSVAGAKLSRGGPREPWHDCHVSVVGGAAWDVLKNFEQRWTKQCNPSVLVNTSG 420

Query: 421 --ILLKLMPPLESNTNPQKDWNVQVFRSINHLSASQVFRNLTVERTIHEAYVEAIRRAER 480
              L+ L  P E N    + WNVQV RSI+H+SA+++ R L VE+++H+ YV AIR+AER
Sbjct: 421 IRNLVNLTGPTEEN---NRKWNVQVLRSIDHISATEMPRGLPVEKSVHDGYVAAIRKAER 480

Query: 481 FIYIENQYFIGGCHLWD--KDQHC-GCTNLIPIEIALKVANKIKAREKFAVYVVIPMWPE 540
           FIYIENQYF+G C  W+   D+ C GCTNLIP+EIALK+A KI+ARE+FAVY+VIPMWPE
Sbjct: 481 FIYIENQYFMGSCDHWESKNDKICSGCTNLIPVEIALKIAAKIRARERFAVYIVIPMWPE 540

Query: 541 GPPESESVEDMLHWTRQTMTMMYRLIGEAIQETGEKAHPRDYLNFFCLANREEERKWDFV 600
           GPPESE+VE++LHWTR+TM+MMY++IGEAI E G+K+HPRDYLNFFCLANREE+R  +F 
Sbjct: 541 GPPESETVEEILHWTRETMSMMYQIIGEAIWEVGDKSHPRDYLNFFCLANREEKRDGEFE 600

Query: 601 PPHSPQHATEYWSAQHHRRFMVYVHSKVMIVDDLYILIGSANVNQRSMDGERDTEIAIGC 660
              SP   T YW+AQ +RRFMVYVHSK+MIVDD YILIGSAN+NQRSMDG RDTEIAIGC
Sbjct: 601 AVSSPHQKTHYWNAQRNRRFMVYVHSKLMIVDDTYILIGSANINQRSMDGCRDTEIAIGC 660

Query: 661 YQLENDGKELPNGRDISTFRLSLWYEHT----QRFEEVFLNPENLQCVERVRSIADESWK 720
           YQ   +     N  +I  +RLSLWYEHT       +     PE+L+CV  +R+I ++ W+
Sbjct: 661 YQTNTN-----NTNEIQAYRLSLWYEHTGGKITADDLSSSEPESLECVRGLRTIGEQMWE 720

Query: 721 IYSGEEVADMKGVHLVTYPVKVKQDGSIEDLEENGGHFPDTKCPIKGRRSMFLPPIFTT 762
           IYSG++V DM G+HLV YP+ V  DG++E++ +  G FPDTK  +KG+RS   PP+ TT
Sbjct: 721 IYSGDKVVDMLGIHLVAYPISVTGDGAVEEVGD--GCFPDTKTLVKGKRSKMFPPVLTT 762

BLAST of CmoCh01G000700 vs. TAIR 10
Match: AT5G25370.1 (phospholipase D alpha 3 )

HSP 1 Score: 604.7 bits (1558), Expect = 9.9e-173
Identity = 341/821 (41.53%), Postives = 476/821 (57.98%), Query Frame = 0

Query: 11  HGTLEVTVFHATTYTPSSPLDCIFSGGKQS------------------------YVTIKI 70
           HGTLEV ++        S  +    G K+                         Y TI +
Sbjct: 8   HGTLEVKIYRIDKLHQRSRFNLCGKGNKEPTGKKTQSQIKRLTDSCTSLFGGHLYATIDL 67

Query: 71  NNIEVARTNHEYDRVWNQTFRVLCAHPLTSTITITMR----TTRSVLGRFQIQAQQILKE 130
           +   VART       W Q+F V  AH + S I  T++     + S++GR  +   +++  
Sbjct: 68  DRSRVARTMMRRHPKWLQSFHVYTAHSI-SKIIFTVKEDEPVSASLIGRAYLPVTEVITG 127

Query: 131 SSFINGFFPLLMENGKP-SPELRLRFMLWFQPAVYELSWKKVLENGEYKGLRNATFPLRS 190
              I+ +  +L EN +P     +L   + F     +++W K +    + G+ NA F  R 
Sbjct: 128 QP-IDRWLDILDENRRPIQGGSKLHVRVKFTHVTQDVNWNKGIILPSFNGVPNAYFNQRE 187

Query: 191 NCHVTLYQDAHHVSTF-QPPFHGSSA---PRRLWEDVYKAIDNAKHLVYIAGWSFNPKMV 250
            C VTLYQDAH ++ +      G        R WE+++ AI  AKHL+YIAGWS N  + 
Sbjct: 188 GCKVTLYQDAHVLNEYPDVTLTGGQVIYKHHRCWEEIFDAIWEAKHLIYIAGWSVNTDVT 247

Query: 251 LVRDPQTHIPYALGVKLGELLKQKAEEGVAVRLMIWDDETSLPIIKNAGIMKTHDEDARA 310
           LVRDP+   P    +KLGELLK+KAEE V V +++WDD TS  + K  G+M THD++   
Sbjct: 248 LVRDPKRTRPGG-DLKLGELLKKKAEENVTVLMLVWDDRTSHEVFKRDGLMMTHDQETYD 307

Query: 311 YFSNSKVVCRLCPKLHPMSPPI---------FSHHQKTITVDAQTHINARNREIMSFIGG 370
           YF N+KV C LCP+       I         F+HHQKTI VD++   +   R I+SF+GG
Sbjct: 308 YFKNTKVRCVLCPRNPDNGDSIVQGFEVATMFTHHQKTIVVDSEVDGSLTKRRIVSFLGG 367

Query: 371 LDLCDGRYDTEQHSLFHTLNTESHCCDFYQTSISGAKLQKGGPREPWHDVHARVTGEAAW 430
           +DLCDGRYDT +H LF TLN+  H  DF+Q +  GA ++KGGPREPWHD+H ++ G AAW
Sbjct: 368 IDLCDGRYDTVEHPLFGTLNS-VHANDFHQPNFDGASIKKGGPREPWHDIHCKLDGPAAW 427

Query: 431 DILTNFEQRWTKQ-SDASLLVPTSILLKL-MPPLE-SNTNPQKDWNVQVFRSINHLSASQ 490
           D+L NFEQRW KQ S    L+  + L ++ +PPL     + ++ W VQVFRSI+   A +
Sbjct: 428 DVLYNFEQRWMKQGSGRRYLISMAQLAEITVPPLPIVQPDNEEGWTVQVFRSIDD-GAVE 487

Query: 491 VF---------------RNLTVERTIHEAYVEAIRRAERFIYIENQYFIGGCHLWDKD-- 550
            F               ++  +ER+I +AYV AIRRA+ FIYIENQYF+G    W+    
Sbjct: 488 GFPEDPREAASIGLISGKDNVIERSIQDAYVNAIRRAKNFIYIENQYFLGSSFGWNSRDI 547

Query: 551 --QHCGCTNLIPIEIALKVANKIKAREKFAVYVVIPMWPEGPPESESVEDMLHWTRQTMT 610
                    LIP EI+LK+ +KI+A E+F+VY+VIP+WPEG P S SV+ +L W R+TM 
Sbjct: 548 NLNEINALQLIPKEISLKIVSKIEAGERFSVYIVIPLWPEGKPGSASVQAILDWQRRTME 607

Query: 611 MMYRLIGEAIQETGEKAHPRDYLNFFCLANREEERKWDFVPPHSPQHATEYWSAQHHRRF 670
           MMY  I  A+++ G  A+PRDYL FFCL NRE+ +  +++PP  P+  ++Y  AQ  RRF
Sbjct: 608 MMYTDIIIALRKKGLDANPRDYLTFFCLGNREKGKVGEYLPPEKPEANSDYARAQESRRF 667

Query: 671 MVYVHSKVMIVDDLYILIGSANVNQRSMDGERDTEIAIGCYQ---LENDGKELPNGRDIS 730
           M+YVHSK+MIVDD YI+IGSAN+NQRSMDG RDTEIA+G YQ   L +     P G+ I 
Sbjct: 668 MIYVHSKMMIVDDEYIIIGSANINQRSMDGGRDTEIAMGAYQPSHLLSTNNMRPVGQ-IF 727

Query: 731 TFRLSLWYEHTQRFEEVFLNPENLQCVERVRSIADESWKIYSGEEVA---DMKGVHLVTY 762
           +FR+SLW EH +     F  PE+ +C+  V + ADE W +YS +E     D+ G HL++Y
Sbjct: 728 SFRISLWLEHLRVTTNAFQCPESEECIRMVNATADELWGLYSAQEYPRNDDLPG-HLLSY 787

BLAST of CmoCh01G000700 vs. TAIR 10
Match: AT3G15730.1 (phospholipase D alpha 1 )

HSP 1 Score: 602.4 bits (1552), Expect = 4.9e-172
Identity = 322/771 (41.76%), Postives = 455/771 (59.01%), Query Frame = 0

Query: 36  GGKQSYVTIKINNIEVART----NHEYDRVWNQTFRVLCAHPLTSTITITMRTTR----S 95
           G  Q Y TI +    V RT    N   +  W ++F + CAH L S I  T++       +
Sbjct: 46  GETQLYATIDLQKARVGRTRKIKNEPKNPKWYESFHIYCAH-LASDIIFTVKDDNPIGAT 105

Query: 96  VLGRFQIQAQQILKESSFINGFFPLLMENGKP-SPELRLRFMLWFQPAVYELSWKKVLEN 155
           ++GR  I   Q++     ++ +  +L  +  P     ++   L +     + +W   +++
Sbjct: 106 LIGRAYIPVDQVINGEE-VDQWVEILDNDRNPIQGGSKIHVKLQYFHVEEDRNWNMGIKS 165

Query: 156 GEYKGLRNATFPLRSNCHVTLYQDAHHVSTFQP--PFHGSS--APRRLWEDVYKAIDNAK 215
            ++ G+    F  R  C V+LYQDAH    F P  P  G     P+R WED++ AI NAK
Sbjct: 166 AKFPGVPYTFFSQRQGCKVSLYQDAHIPDNFVPRIPLAGGKNYEPQRCWEDIFDAISNAK 225

Query: 216 HLVYIAGWSFNPKMVLVRDPQTHIPYALGVKLGELLKQKAEEGVAVRLMIWDDETSLPII 275
           HL+YI GWS   ++ LVRD +   P    V +GELLK+KA EGV V L++WDD TS+ ++
Sbjct: 226 HLIYITGWSVYAEIALVRDSRRPKPGG-DVTIGELLKKKASEGVRVLLLVWDDRTSVDVL 285

Query: 276 KNAGIMKTHDEDARAYFSNSKVVCRLCPKLHP----------MSPPIFSHHQKTITVDAQ 335
           K  G+M THDE+   +F  S V C LCP+ +P              +F+HHQK + VD++
Sbjct: 286 KKDGLMATHDEETENFFRGSDVHCILCPR-NPDDGGSIVQSLQISTMFTHHQKIVVVDSE 345

Query: 336 --THINARNREIMSFIGGLDLCDGRYDTEQHSLFHTLNTESHCCDFYQTSISGAKLQKGG 395
             +   +  R I+SF+GG+DLCDGRYDT  HSLF TL+T  H  DF+Q + +GA + KGG
Sbjct: 346 MPSRGGSEMRRIVSFVGGIDLCDGRYDTPFHSLFRTLDTVHH-DDFHQPNFTGAAITKGG 405

Query: 396 PREPWHDVHARVTGEAAWDILTNFEQRWTKQSDASLLVPTSIL--LKLMPPLESNTNPQK 455
           PREPWHD+H+R+ G  AWD++ NFEQRW+KQ    +LV    L  + + P          
Sbjct: 406 PREPWHDIHSRLEGPIAWDVMYNFEQRWSKQGGKDILVKLRDLSDIIITPSPVMFQEDHD 465

Query: 456 DWNVQVFRSINHLSASQV--------------FRNLTVERTIHEAYVEAIRRAERFIYIE 515
            WNVQ+FRSI+  +A+                 ++  ++R+I +AY+ AIRRA+ FIY+E
Sbjct: 466 VWNVQLFRSIDGGAAAGFPESPEAAAEAGLVSGKDNIIDRSIQDAYIHAIRRAKDFIYVE 525

Query: 516 NQYFIGGCHLWDKD----QHCGCTNLIPIEIALKVANKIKAREKFAVYVVIPMWPEGPPE 575
           NQYF+G    W  D    +     +LIP E++LK+ +KI+  EKF VYVV+PMWPEG PE
Sbjct: 526 NQYFLGSSFAWAADGITPEDINALHLIPKELSLKIVSKIEKGEKFRVYVVVPMWPEGLPE 585

Query: 576 SESVEDMLHWTRQTMTMMYRLIGEAIQETGEKAHPRDYLNFFCLANREEERKWDFVPPHS 635
           S SV+ +L W R+TM MMY+ + +A++  G +  PR+YL FFCL NRE ++  ++ P   
Sbjct: 586 SGSVQAILDWQRRTMEMMYKDVIQALRAQGLEEDPRNYLTFFCLGNREVKKDGEYEPAEK 645

Query: 636 PQHATEYWSAQHHRRFMVYVHSKVMIVDDLYILIGSANVNQRSMDGERDTEIAIGCYQLE 695
           P   T+Y  AQ  RRFM+YVH+K+MIVDD YI+IGSAN+NQRSMDG RD+EIA+G YQ  
Sbjct: 646 PDPDTDYMRAQEARRFMIYVHTKMMIVDDEYIIIGSANINQRSMDGARDSEIAMGGYQPH 705

Query: 696 NDGKELPNGRDISTFRLSLWYEHTQRFEEVFLNPENLQCVERVRSIADESWKIYSGEEVA 755
           +     P    I  FR+SLWYEH    +E FL+P +L+C+E+V  I+D+ W  YS E + 
Sbjct: 706 HLSHRQPARGQIHGFRMSLWYEHLGMLDETFLDPSSLECIEKVNRISDKYWDFYSSESLE 765

Query: 756 DMKGVHLVTYPVKVKQDGSIEDLEENGGHFPDTKCPIKGRRSMFLPPIFTT 762
                HL+ YP+ V  +G I +L      FPDTK  I G +S +LPPI TT
Sbjct: 766 HDLPGHLLRYPIGVASEGDITEL-PGFEFFPDTKARILGTKSDYLPPILTT 810

BLAST of CmoCh01G000700 vs. TAIR 10
Match: AT1G52570.1 (phospholipase D alpha 2 )

HSP 1 Score: 596.7 bits (1537), Expect = 2.7e-170
Identity = 322/770 (41.82%), Postives = 456/770 (59.22%), Query Frame = 0

Query: 36  GGKQSYVTIKINNIEVAR----TNHEYDRVWNQTFRVLCAHPLTSTITITMRTTR----S 95
           G  Q Y TI +    V R    T    +  W ++F + C H +   +  T++       +
Sbjct: 46  GETQLYATIDLEKARVGRTRKITKEPKNPKWFESFHIYCGH-MAKHVIFTVKDANPIGAT 105

Query: 96  VLGRFQIQAQQILKESSFINGFFPLLMENGKPSPELRLRFMLWFQPAVYELSWKKVLENG 155
           ++GR  I  + IL           L  E    +   ++   L +     + +W + +++ 
Sbjct: 106 LIGRGYIPVEDILHGEEVDRWVDILDNEKNPIAGGSKIHVKLQYFGVEKDKNWNRGIKSA 165

Query: 156 EYKGLRNATFPLRSNCHVTLYQDAHHVSTFQP--PFHGSS--APRRLWEDVYKAIDNAKH 215
           ++ G+    F  R  C V+LYQDAH    F P  P  G     P R WED++ AI NAKH
Sbjct: 166 KFPGVPYTFFSQRRGCKVSLYQDAHIPGNFVPKIPLAGGKNYEPHRCWEDIFDAITNAKH 225

Query: 216 LVYIAGWSFNPKMVLVRDPQTHIPYALG-VKLGELLKQKAEEGVAVRLMIWDDETSLPII 275
           L+YI GWS   ++ LVRD +   P   G V +GELLK+KA EGV V L++WDD TS+ ++
Sbjct: 226 LIYITGWSVYTEISLVRDSRR--PKQGGDVTVGELLKKKASEGVKVILLVWDDRTSVDLL 285

Query: 276 KNAGIMKTHDEDARAYFSNSKVVCRLCPK---------LHPMSPPIFSHHQKTITVDAQT 335
           K  G+M THDE+   +F  + V C LCP+          +     +F+HHQK + VD++ 
Sbjct: 286 KKDGLMATHDEETENFFRGTDVNCILCPRNPDDGGSIVQNLQISTMFTHHQKIVVVDSEM 345

Query: 336 HI-NARNREIMSFIGGLDLCDGRYDTEQHSLFHTLNTESHCCDFYQTSISGAKLQKGGPR 395
               +R+R I+SF+GGLDLCDGRYDT  HSLF TL+T +H  DF+Q + +GA + KGGPR
Sbjct: 346 PSGGSRSRRIVSFVGGLDLCDGRYDTPFHSLFRTLDT-AHHDDFHQPNFTGAAITKGGPR 405

Query: 396 EPWHDVHARVTGEAAWDILTNFEQRWTKQSDASLLVPTSILLK-LMPPLESNTNPQKD-W 455
           EPWHD+H R+ G  AWD+L NFEQRW++Q    +LV    L   ++PP     +   D W
Sbjct: 406 EPWHDIHCRLEGPIAWDVLYNFEQRWSRQGGKDILVKMRELGDIIIPPSPVLFSEDHDVW 465

Query: 456 NVQVFRSINHLSASQV--------------FRNLTVERTIHEAYVEAIRRAERFIYIENQ 515
           NVQ+FRSI+  +A+                 ++  ++R+I +AY+ AIRRA+ FIYIENQ
Sbjct: 466 NVQLFRSIDGGAAAGFPDSPEAAAEAGLVSGKDNIIDRSIQDAYIHAIRRAKDFIYIENQ 525

Query: 516 YFIGGCHLWDKD----QHCGCTNLIPIEIALKVANKIKAREKFAVYVVIPMWPEGPPESE 575
           YF+G    W  D    +     +LIP E++LK+ +KIKA EKF VYVV+PMWPEG PES 
Sbjct: 526 YFLGSSFAWSADGIKPEEINALHLIPKELSLKIVSKIKAGEKFKVYVVVPMWPEGIPESG 585

Query: 576 SVEDMLHWTRQTMTMMYRLIGEAIQETG-EKAHPRDYLNFFCLANREEERKWDFVPPHSP 635
           SV+ +L W ++TM MMY+ + +A++E G E   PRDYL FFCL NRE ++  ++ P   P
Sbjct: 586 SVQAILDWQKRTMEMMYKDVIKALRENGLEGEDPRDYLTFFCLGNREVKKDGEYEPSEKP 645

Query: 636 QHATEYWSAQHHRRFMVYVHSKVMIVDDLYILIGSANVNQRSMDGERDTEIAIGCYQLEN 695
           +  T+Y  AQ  RRFM+YVH+K+MIVDD YI+IGSAN+NQRSMDG RD+EIA+G YQ  +
Sbjct: 646 EPDTDYIRAQEARRFMIYVHTKMMIVDDEYIIIGSANINQRSMDGARDSEIAMGGYQPYH 705

Query: 696 DGKELPNGRDISTFRLSLWYEHTQRFEEVFLNPENLQCVERVRSIADESWKIYSGEEVAD 755
                P    I  FR+SLWYEH    +E FL+P + +C+++V  +AD+ W +YS E +  
Sbjct: 706 LSTRQPARGQIHGFRMSLWYEHLGMLDETFLDPSSQECIQKVNRVADKYWDLYSSESLEH 765

Query: 756 MKGVHLVTYPVKVKQDGSIEDLEENGGHFPDTKCPIKGRRSMFLPPIFTT 762
               HL+ YP+ +  +G+I +L      FPDTK  I G +S ++PPI TT
Sbjct: 766 DLPGHLLRYPIGIASEGNITEL-PGCEFFPDTKARILGVKSDYMPPILTT 810

BLAST of CmoCh01G000700 vs. TAIR 10
Match: AT2G42010.1 (phospholipase D beta 1 )

HSP 1 Score: 524.6 bits (1350), Expect = 1.3e-148
Identity = 314/769 (40.83%), Postives = 433/769 (56.31%), Query Frame = 0

Query: 41   YVTIKINNIEVART---NHEYDRVWNQTFRVLCAHPLTSTITITMRTT---RSVLGRFQI 100
            YV++ +    + RT   ++  + VW Q F V  AH       +   +      ++G   I
Sbjct: 319  YVSVSVAGAVIGRTYVMSNSENPVWMQHFYVPVAHHAAEVHFVVKDSDVVGSQLIGLVTI 378

Query: 101  QAQQILKESSFINGFFPLLMENGKP-SPELRLRFMLWFQP----AVYELSWKKVLENGEY 160
              +QI   +  I G +P+L  NGKP  P   L   + + P    +VY      V    +Y
Sbjct: 379  PVEQIYSGAK-IEGTYPILNSNGKPCKPGANLSLSIQYTPMDKLSVYH---HGVGAGPDY 438

Query: 161  KGLRNATFPLRSNCHVTLYQDAHHVSTFQPPF---HGSSAPR-RLWEDVYKAIDNAKHLV 220
            +G+    FPLR    V LYQDAH      P     +G S    + W D++ AI  A+ L+
Sbjct: 439  QGVPGTYFPLRKGGTVRLYQDAHVPEGMLPGIRLDNGMSYEHGKCWHDMFDAIRQARRLI 498

Query: 221  YIAGWSFNPKMVLVRDPQTHIPYALGVKLGELLKQKAEEGVAVRLMIWDDETSLPII--K 280
            YI GWS   K+ L+RD    +  A    LGELL+ K++EGV V L+IWDD TS  I+  K
Sbjct: 499  YITGWSVWHKVKLIRD---KLGPASECTLGELLRSKSQEGVRVLLLIWDDPTSRSILGYK 558

Query: 281  NAGIMKTHDEDARAYFSNSKVVCRLCP----KLHPMSP-----PIFSHHQKTITVDAQTH 340
              G+M THDE+ R +F +S V   LCP    K H          I++HHQK + VDA   
Sbjct: 559  TDGVMATHDEETRRFFKHSSVQVLLCPRNAGKRHSWVKQREVGTIYTHHQKNVIVDADA- 618

Query: 341  INARNREIMSFIGGLDLCDGRYDTEQHSLFHTLNTESHCCDFYQTSISGAKLQKGGPREP 400
                 R+I++F+GGLDLCDGRYDT QH LF TL T  H  DF+  + +G     G PREP
Sbjct: 619  -GGNRRKIIAFVGGLDLCDGRYDTPQHPLFRTLQT-IHKDDFHNPTFTGN--LSGCPREP 678

Query: 401  WHDVHARVTGEAAWDILTNFEQRW-------------TKQSDASLLVP--TSILLKLMPP 460
            WHD+H+++ G AA+D+LTNFE+RW             T   DA L +     IL     P
Sbjct: 679  WHDLHSKIDGPAAYDVLTNFEERWLKAAKPSGIKKFKTSYDDALLRIDRIPDILGVSDTP 738

Query: 461  LESNTNPQKDWNVQVFRSINHLSAS--------------QVFRNLTVERTIHEAYVEAIR 520
              S  +P+  W+VQ+FRSI+  S                   +N+ ++ +IH AYV+AIR
Sbjct: 739  TVSENDPEA-WHVQIFRSIDSNSVKGFPKDPKDATCKNLVCGKNVLIDMSIHTAYVKAIR 798

Query: 521  RAERFIYIENQYFIGGCHLWDKDQHCGCTNLIPIEIALKVANKIKAREKFAVYVVIPMWP 580
             A+ FIYIENQYFIG  + W+  +  G  NLIP+EIALK+A KI+A E+FA Y+VIPMWP
Sbjct: 799  AAQHFIYIENQYFIGSSYNWNAHKDIGANNLIPMEIALKIAEKIRANERFAAYIVIPMWP 858

Query: 581  EGPPESESVEDMLHWTRQTMTMMYRLIGEAIQETGEKA--HPRDYLNFFCLANREEERKW 640
            EG P   + + +L+W  +T+ MMY  I +A+ ETG +    P+DYLNFFCL NRE     
Sbjct: 859  EGVPTGAATQRILYWQHKTIQMMYETIYKALVETGLEGAFSPQDYLNFFCLGNREMVDGI 918

Query: 641  DFVPPHSPQHA-TEYWSAQHHRRFMVYVHSKVMIVDDLYILIGSANVNQRSMDGERDTEI 700
            D     SP +A T    ++  RRFMVYVHSK M+VDD Y++IGSAN+NQRSM+G RDTEI
Sbjct: 919  DNSGTGSPSNANTPQALSRKSRRFMVYVHSKGMVVDDEYVVIGSANINQRSMEGTRDTEI 978

Query: 701  AIGCYQLENDGKELPNG--RDISTFRLSLWYEHTQRFEEVFLNPENLQCVERVRSIADES 750
            A+G YQ ++      +G    I  +R+SLW EH    ++ F  PE+++CV +VR++ + +
Sbjct: 979  AMGAYQPQHTWARKHSGPRGQIYGYRMSLWAEHMATLDDCFTQPESIECVRKVRTMGERN 1038

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9C8881.0e-28360.08Phospholipase D alpha 4 OS=Arabidopsis thaliana OX=3702 GN=PLDALPHA4 PE=2 SV=1[more]
Q432707.6e-17841.11Phospholipase D alpha 1 OS=Zea mays OX=4577 GN=PLD1 PE=2 SV=1[more]
Q430071.1e-17641.18Phospholipase D alpha 1 OS=Oryza sativa subsp. japonica OX=39947 GN=PLD1 PE=1 SV... [more]
P934002.1e-17240.35Phospholipase D alpha 1 OS=Nicotiana tabacum OX=4097 GN=PLD1 PE=2 SV=2[more]
Q70EW53.7e-17240.10Phospholipase D alpha 1 OS=Cynara cardunculus OX=4265 GN=PLD1 PE=1 SV=2[more]
Match NameE-valueIdentityDescription
A0A6J1G9V50.0e+00100.00Phospholipase D OS=Cucurbita moschata OX=3662 GN=LOC111452112 PE=3 SV=1[more]
A0A6J1KAT10.0e+0098.82Phospholipase D OS=Cucurbita maxima OX=3661 GN=LOC111492613 PE=3 SV=1[more]
A0A5D3BW810.0e+0090.14Phospholipase D OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold863G0013... [more]
A0A1S3BH900.0e+0090.14Phospholipase D OS=Cucumis melo OX=3656 GN=LOC103490014 PE=3 SV=1[more]
A0A6J1D3G40.0e+0088.44Phospholipase D OS=Momordica charantia OX=3673 GN=LOC111016958 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
XP_022948424.10.0e+00100.00phospholipase D alpha 4 [Cucurbita moschata][more]
KAG7036437.10.0e+0099.87Phospholipase D alpha 4 [Cucurbita argyrosperma subsp. argyrosperma][more]
KAG6606722.10.0e+0099.61Phospholipase D alpha 4, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_023525777.10.0e+0098.95phospholipase D alpha 4 [Cucurbita pepo subsp. pepo][more]
XP_022997750.10.0e+0098.82phospholipase D alpha 4 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
AT1G55180.17.3e-28560.08phospholipase D alpha 4 [more]
AT5G25370.19.9e-17341.53phospholipase D alpha 3 [more]
AT3G15730.14.9e-17241.76phospholipase D alpha 1 [more]
AT1G52570.12.7e-17041.82phospholipase D alpha 2 [more]
AT2G42010.11.3e-14840.83phospholipase D beta 1 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001736Phospholipase D/TransphosphatidylaseSMARTSM00155pld_4coord: 300..335
e-value: 59.0
score: 6.9
coord: 606..633
e-value: 6.9E-6
score: 35.6
IPR001736Phospholipase D/TransphosphatidylasePFAMPF00614PLDccoord: 609..633
e-value: 5.4E-5
score: 23.2
coord: 300..335
e-value: 1.1E-5
score: 25.3
IPR001736Phospholipase D/TransphosphatidylasePROSITEPS50035PLDcoord: 606..633
score: 14.438799
IPR024632Phospholipase D, C-terminalPFAMPF12357PLD_Ccoord: 678..750
e-value: 1.9E-24
score: 85.3
NoneNo IPR availableGENE3D3.30.870.10Endonuclease Chain Acoord: 170..431
e-value: 2.5E-25
score: 90.8
NoneNo IPR availableGENE3D3.30.870.10Endonuclease Chain Acoord: 445..652
e-value: 9.1E-12
score: 46.8
NoneNo IPR availablePANTHERPTHR18896:SF137PHOSPHOLIPASE D ALPHA 4coord: 7..761
NoneNo IPR availableSUPERFAMILY56024Phospholipase D/nucleasecoord: 150..409
NoneNo IPR availableSUPERFAMILY56024Phospholipase D/nucleasecoord: 425..688
IPR035892C2 domain superfamilyGENE3D2.60.40.150C2 domaincoord: 2..131
e-value: 1.3E-5
score: 26.9
IPR035892C2 domain superfamilySUPERFAMILY49562C2 domain (Calcium/lipid-binding domain, CaLB)coord: 8..129
IPR011402Phospholipase D, plantPIRSFPIRSF036470PLD_plantcoord: 2..761
e-value: 1.0E-271
score: 901.4
IPR000008C2 domainPFAMPF00168C2coord: 12..112
e-value: 6.9E-6
score: 26.3
IPR015679Phospholipase D familyPANTHERPTHR18896PHOSPHOLIPASE Dcoord: 7..761

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh01G000700.1CmoCh01G000700.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0046470 phosphatidylcholine metabolic process
biological_process GO:0009395 phospholipid catabolic process
cellular_component GO:0005886 plasma membrane
cellular_component GO:0016020 membrane
molecular_function GO:0005509 calcium ion binding
molecular_function GO:0070290 N-acylphosphatidylethanolamine-specific phospholipase D activity
molecular_function GO:0004630 phospholipase D activity
molecular_function GO:0003824 catalytic activity