Cmc12g0336351 (gene) Melon (Charmono) v1.1

Overview


Name	Cmc12g0336351
Type	gene
Organism	Cucumis melo L. var. cantalupensis cv. Charmono (Melon (Charmono) v1.1)
Description	kunitz trypsin inhibitor 2
Location	CMiso1.1chr12: 24925151 .. 24926162 (+)
RNA-Seq Expression	Cmc12g0336351
Synteny	Cmc12g0336351

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TTTTGCAATGTGAATATCAAACTCATGCAAATGTACCGCCACGTCATGGCGGCGGGTTGGAAAACGATGCATCTAAGATAAGGAGAGAAAATAGTGAGCAATTTCGACTCTCGCTCGCTCTCCTATAAATACAAAAACTTTCCCAATCAAATTAAGCATCCAAACAAAACAAAGTCCACATTCAAAAGAAAAATGACGAAGAATTTCGGAATATTCTATTTTATTTTCATTCTCCTTGCCTCAACCGAGCTCCGCTTCTCCACAGCCGACGCTTCACCGGAGGCCGTCCTCGACATCGACGGCAAGAAGCTCCGAGCCGGCGTCAACTACTACATCCTCCCTGTTTTCCGCGGCAGAGGCGGCGGCCTAACTCTAGGCAACCTCCAATCGGAGATATGTCCAGTCAACGTCGTTCAAGAACAATTCGAATTAATGAACGGATTTCCAACAACATTCCATCCTGTAAACCCTAAAAAAGGAGTGGTTCGAGTTTCAACCGATTTGAATGTGCAATTCGATGCGAGTACGATCTGCGTGACATCGACGGTGTGGAAATTGGACAAATTCGACGAATCGACGGGACAATGGTTCGTGACGATCGGCGGAAGCAGGGGAAATCCGGGAGTGGAAACGGTGGATAATTGGTTCAAAATTGAGAAGCACGGTAAGGATTACAAATTGGTGTTCTGTCCGACTGTTTGTAATTTCTGTAAAGTTATGTGTAGAGATATTGGAATCTTCTTCAAGAATGGAAAAAGGGCTTTGGCTTTGAGCGATACGCCATTCCCTGTTATGTTCAAGAAAGTTTAATTGAAGAAAATTCAAAAATTAAAGTGAATGAGTTTATGAAGTAATTTGTGTTTAATTCATGTATAAGAGTTTTATCATAATTAAATAAAAGATATGGTTATTGTTTGTTTATTATTATTAGAAGAATAAAATACTTTATTTGATTATTTTCGTGTAAGCTGTTTTCACCTCAGTTTCTCAAAATCAAAGTACGTCTTATTTT

mRNA sequence

TTTTGCAATGTGAATATCAAACTCATGCAAATGTACCGCCACGTCATGGCGGCGGGTTGGAAAACGATGCATCTAAGATAAGGAGAGAAAATAGTGAGCAATTTCGACTCTCGCTCGCTCTCCTATAAATACAAAAACTTTCCCAATCAAATTAAGCATCCAAACAAAACAAAGTCCACATTCAAAAGAAAAATGACGAAGAATTTCGGAATATTCTATTTTATTTTCATTCTCCTTGCCTCAACCGAGCTCCGCTTCTCCACAGCCGACGCTTCACCGGAGGCCGTCCTCGACATCGACGGCAAGAAGCTCCGAGCCGGCGTCAACTACTACATCCTCCCTGTTTTCCGCGGCAGAGGCGGCGGCCTAACTCTAGGCAACCTCCAATCGGAGATATGTCCAGTCAACGTCGTTCAAGAACAATTCGAATTAATGAACGGATTTCCAACAACATTCCATCCTGTAAACCCTAAAAAAGGAGTGGTTCGAGTTTCAACCGATTTGAATGTGCAATTCGATGCGAGTACGATCTGCGTGACATCGACGGTGTGGAAATTGGACAAATTCGACGAATCGACGGGACAATGGTTCGTGACGATCGGCGGAAGCAGGGGAAATCCGGGAGTGGAAACGGTGGATAATTGGTTCAAAATTGAGAAGCACGGTAAGGATTACAAATTGGTGTTCTGTCCGACTGTTTGTAATTTCTGTAAAGTTATGTGTAGAGATATTGGAATCTTCTTCAAGAATGGAAAAAGGGCTTTGGCTTTGAGCGATACGCCATTCCCTGTTATGTTCAAGAAAGTTTAATTGAAGAAAATTCAAAAATTAAAGTGAATGAGTTTATGAAGTAATTTGTGTTTAATTCATGTATAAGAGTTTTATCATAATTAAATAAAAGATATGGTTATTGTTTGTTTATTATTATTAGAAGAATAAAATACTTTATTTGATTATTTTCGTGTAAGCTGTTTTCACCTCAGTTTCTCAAAATCAAAGTACGTCTTATTTT

Coding sequence (CDS)

ATGACGAAGAATTTCGGAATATTCTATTTTATTTTCATTCTCCTTGCCTCAACCGAGCTCCGCTTCTCCACAGCCGACGCTTCACCGGAGGCCGTCCTCGACATCGACGGCAAGAAGCTCCGAGCCGGCGTCAACTACTACATCCTCCCTGTTTTCCGCGGCAGAGGCGGCGGCCTAACTCTAGGCAACCTCCAATCGGAGATATGTCCAGTCAACGTCGTTCAAGAACAATTCGAATTAATGAACGGATTTCCAACAACATTCCATCCTGTAAACCCTAAAAAAGGAGTGGTTCGAGTTTCAACCGATTTGAATGTGCAATTCGATGCGAGTACGATCTGCGTGACATCGACGGTGTGGAAATTGGACAAATTCGACGAATCGACGGGACAATGGTTCGTGACGATCGGCGGAAGCAGGGGAAATCCGGGAGTGGAAACGGTGGATAATTGGTTCAAAATTGAGAAGCACGGTAAGGATTACAAATTGGTGTTCTGTCCGACTGTTTGTAATTTCTGTAAAGTTATGTGTAGAGATATTGGAATCTTCTTCAAGAATGGAAAAAGGGCTTTGGCTTTGAGCGATACGCCATTCCCTGTTATGTTCAAGAAAGTTTAA

Protein sequence

MTKNFGIFYFIFILLASTELRFSTADASPEAVLDIDGKKLRAGVNYYILPVFRGRGGGLTLGNLQSEICPVNVVQEQFELMNGFPTTFHPVNPKKGVVRVSTDLNVQFDASTICVTSTVWKLDKFDESTGQWFVTIGGSRGNPGVETVDNWFKIEKHGKDYKLVFCPTVCNFCKVMCRDIGIFFKNGKRALALSDTPFPVMFKKV

Homology

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BLAST of Cmc12g0336351 vs. NCBI nr
Match: XP_008437058.1 (PREDICTED: miraculin-like [Cucumis melo] >TYJ99073.1 miraculin-like [Cucumis melo var. makuwa])

HSP 1 Score: 430.3 bits (1105), Expect = 9.6e-117
Identity = 205/205 (100.00%), Postives = 205/205 (100.00%), Query Frame = 0


Query: 1   MTKNFGIFYFIFILLASTELRFSTADASPEAVLDIDGKKLRAGVNYYILPVFRGRGGGLT 60
           MTKNFGIFYFIFILLASTELRFSTADASPEAVLDIDGKKLRAGVNYYILPVFRGRGGGLT
Sbjct: 1   MTKNFGIFYFIFILLASTELRFSTADASPEAVLDIDGKKLRAGVNYYILPVFRGRGGGLT 60

Query: 61  LGNLQSEICPVNVVQEQFELMNGFPTTFHPVNPKKGVVRVSTDLNVQFDASTICVTSTVW 120
           LGNLQSEICPVNVVQEQFELMNGFPTTFHPVNPKKGVVRVSTDLNVQFDASTICVTSTVW
Sbjct: 61  LGNLQSEICPVNVVQEQFELMNGFPTTFHPVNPKKGVVRVSTDLNVQFDASTICVTSTVW 120

Query: 121 KLDKFDESTGQWFVTIGGSRGNPGVETVDNWFKIEKHGKDYKLVFCPTVCNFCKVMCRDI 180
           KLDKFDESTGQWFVTIGGSRGNPGVETVDNWFKIEKHGKDYKLVFCPTVCNFCKVMCRDI
Sbjct: 121 KLDKFDESTGQWFVTIGGSRGNPGVETVDNWFKIEKHGKDYKLVFCPTVCNFCKVMCRDI 180

Query: 181 GIFFKNGKRALALSDTPFPVMFKKV 206
           GIFFKNGKRALALSDTPFPVMFKKV
Sbjct: 181 GIFFKNGKRALALSDTPFPVMFKKV 205

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BLAST of Cmc12g0336351 vs. NCBI nr
Match: XP_004137534.1 (kunitz trypsin inhibitor 5 [Cucumis sativus])

HSP 1 Score: 402.5 bits (1033), Expect = 2.2e-108
Identity = 194/205 (94.63%), Postives = 200/205 (97.56%), Query Frame = 0


Query: 3   KNFGI-FYFIFILLASTEL-RFSTADASPEAVLDIDGKKLRAGVNYYILPVFRGRGGGLT 62
           KNFGI FYF+FILLAST+L RFSTADASPEAVLDIDGKKLRAGVNYYILPVFRGRGGGLT
Sbjct: 2   KNFGILFYFLFILLASTQLIRFSTADASPEAVLDIDGKKLRAGVNYYILPVFRGRGGGLT 61

Query: 63  LGNLQSEICPVNVVQEQFELMNGFPTTFHPVNPKKGVVRVSTDLNVQFDASTICVTSTVW 122
           LGNLQSE CP+NVVQEQ E+MNGFPTTFHPVNPKKGVVRVSTDLNVQF+ASTICVTSTVW
Sbjct: 62  LGNLQSEKCPLNVVQEQLEVMNGFPTTFHPVNPKKGVVRVSTDLNVQFEASTICVTSTVW 121

Query: 123 KLDKFDESTGQWFVTIGGSRGNPGVETVDNWFKIEKHGKDYKLVFCPTVCNFCKVMCRDI 182
           KLDKFDESTGQW VTIGGSRGNPGVETVDNWFKIEKHGKDYKLVFCPTVCNFCKVMCRDI
Sbjct: 122 KLDKFDESTGQWLVTIGGSRGNPGVETVDNWFKIEKHGKDYKLVFCPTVCNFCKVMCRDI 181

Query: 183 GIFFKNGKRALALSDTPFPVMFKKV 206
           GIFFKNG+RALALSDTPFPVMFKKV
Sbjct: 182 GIFFKNGERALALSDTPFPVMFKKV 206

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BLAST of Cmc12g0336351 vs. NCBI nr
Match: XP_038895203.1 (kunitz trypsin inhibitor 5-like [Benincasa hispida])

HSP 1 Score: 386.3 bits (991), Expect = 1.6e-103
Identity = 187/204 (91.67%), Postives = 192/204 (94.12%), Query Frame = 0


Query: 3   KNFGIFY-FIFILLASTELRFSTADASPEAVLDIDGKKLRAGVNYYILPVFRGRGGGLTL 62
           KNFGI   F+FILLASTE+RFS ADASPEAV DIDGKKLRAGVNYYILPV RGRGGGLTL
Sbjct: 2   KNFGILLSFLFILLASTEVRFSRADASPEAVRDIDGKKLRAGVNYYILPVIRGRGGGLTL 61

Query: 63  GNLQSEICPVNVVQEQFELMNGFPTTFHPVNPKKGVVRVSTDLNVQFDASTICVTSTVWK 122
            NLQSE CPVNVVQEQFELMNGFPTTF PVNPKKGVVRVSTDLNVQF+ASTIC TSTVWK
Sbjct: 62  SNLQSENCPVNVVQEQFELMNGFPTTFLPVNPKKGVVRVSTDLNVQFEASTICATSTVWK 121

Query: 123 LDKFDESTGQWFVTIGGSRGNPGVETVDNWFKIEKHGKDYKLVFCPTVCNFCKVMCRDIG 182
           LDK DESTGQWFVTIGGSRGNPGVETVDNWFKI KHGKDYKLVFCP+VC+FCKVMCRDIG
Sbjct: 122 LDKVDESTGQWFVTIGGSRGNPGVETVDNWFKIVKHGKDYKLVFCPSVCDFCKVMCRDIG 181

Query: 183 IFFKNGKRALALSDTPFPVMFKKV 206
           IFFKNGKRALALSDTPFPVMFKKV
Sbjct: 182 IFFKNGKRALALSDTPFPVMFKKV 205

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BLAST of Cmc12g0336351 vs. NCBI nr
Match: KAE8652565.1 (hypothetical protein Csa_014019 [Cucumis sativus])

HSP 1 Score: 381.3 bits (978), Expect = 5.1e-102
Identity = 178/186 (95.70%), Postives = 183/186 (98.39%), Query Frame = 0


Query: 20  LRFSTADASPEAVLDIDGKKLRAGVNYYILPVFRGRGGGLTLGNLQSEICPVNVVQEQFE 79
           +RFSTADASPEAVLDIDGKKLRAGVNYYILPVFRGRGGGLTLGNLQSE CP+NVVQEQ E
Sbjct: 63  IRFSTADASPEAVLDIDGKKLRAGVNYYILPVFRGRGGGLTLGNLQSEKCPLNVVQEQLE 122

Query: 80  LMNGFPTTFHPVNPKKGVVRVSTDLNVQFDASTICVTSTVWKLDKFDESTGQWFVTIGGS 139
           +MNGFPTTFHPVNPKKGVVRVSTDLNVQF+ASTICVTSTVWKLDKFDESTGQW VTIGGS
Sbjct: 123 VMNGFPTTFHPVNPKKGVVRVSTDLNVQFEASTICVTSTVWKLDKFDESTGQWLVTIGGS 182

Query: 140 RGNPGVETVDNWFKIEKHGKDYKLVFCPTVCNFCKVMCRDIGIFFKNGKRALALSDTPFP 199
           RGNPGVETVDNWFKIEKHGKDYKLVFCPTVCNFCKVMCRDIGIFFKNG+RALALSDTPFP
Sbjct: 183 RGNPGVETVDNWFKIEKHGKDYKLVFCPTVCNFCKVMCRDIGIFFKNGERALALSDTPFP 242

Query: 200 VMFKKV 206
           VMFKKV
Sbjct: 243 VMFKKV 248

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BLAST of Cmc12g0336351 vs. NCBI nr
Match: XP_022923703.1 (kunitz trypsin inhibitor 2 [Cucurbita moschata] >XP_023001808.1 kunitz trypsin inhibitor 2 [Cucurbita maxima] >KAG6584289.1 Kunitz trypsin inhibitor 5, partial [Cucurbita argyrosperma subsp. sororia] >KAG7019882.1 Kunitz trypsin inhibitor 2, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 364.4 bits (934), Expect = 6.5e-97
Identity = 172/203 (84.73%), Postives = 185/203 (91.13%), Query Frame = 0


Query: 3   KNFGIFYFIFILLASTELRFSTADASPEAVLDIDGKKLRAGVNYYILPVFRGRGGGLTLG 62
           KNF +  F+FILLAS E+R S ADASP+AV DIDGKKLRAGVNYYILPVFRGRGGGL LG
Sbjct: 2   KNFALLSFLFILLAS-EVRVSRADASPDAVRDIDGKKLRAGVNYYILPVFRGRGGGLALG 61

Query: 63  NLQSEICPVNVVQEQFELMNGFPTTFHPVNPKKGVVRVSTDLNVQFDASTICVTSTVWKL 122
           NLQS+ CP+NVVQEQFELMNG P  F PVNPKKGVVRVSTDLNVQF+ASTIC TSTVWKL
Sbjct: 62  NLQSDKCPLNVVQEQFELMNGLPAAFLPVNPKKGVVRVSTDLNVQFEASTICATSTVWKL 121

Query: 123 DKFDESTGQWFVTIGGSRGNPGVETVDNWFKIEKHGKDYKLVFCPTVCNFCKVMCRDIGI 182
           DKFDEST QWF+TIGG+RGNPGV+TVDNWFKIEKHG DYK  FCPTVC+FCKVMCRD+GI
Sbjct: 122 DKFDESTKQWFITIGGTRGNPGVKTVDNWFKIEKHGNDYKFKFCPTVCDFCKVMCRDVGI 181

Query: 183 FFKNGKRALALSDTPFPVMFKKV 206
           FFKNGKRALALSDTPFPVMFK+V
Sbjct: 182 FFKNGKRALALSDTPFPVMFKEV 203

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BLAST of Cmc12g0336351 vs. ExPASy Swiss-Prot
Match: Q9LMU2 (Kunitz trypsin inhibitor 5 OS=Arabidopsis thaliana OX=3702 GN=KTI5 PE=2 SV=1)

HSP 1 Score: 232.3 bits (591), Expect = 5.1e-60
Identity = 110/196 (56.12%), Postives = 142/196 (72.45%), Query Frame = 0


Query: 10  FIFILLA-STELRFSTADASPEAVLDIDGKKLRAGVNYYILPVFRGRGGGLTLGNLQSEI 69
           +IF+LLA     R  T +A+ E V DI+GK L  GVNYYILPV RGRGGGLT+ NL++E 
Sbjct: 6   YIFLLLAVFISHRGVTTEAAVEPVKDINGKSLLTGVNYYILPVIRGRGGGLTMSNLKTET 65

Query: 70  CPVNVVQEQFELMNGFPTTFHPVNPKKGVVRVSTDLNVQFDASTICVTSTVWKLDKFDES 129
           CP +V+Q+QFE+  G P  F P + K   + VSTD+N++F  ++I      W+L  FDE+
Sbjct: 66  CPTSVIQDQFEVSQGLPVKFSPYD-KSRTIPVSTDVNIKFSPTSI------WELANFDET 125

Query: 130 TGQWFVTIGGSRGNPGVETVDNWFKIEKHGKDYKLVFCPTVCNFCKVMCRDIGIFFKNGK 189
           T QWF++  G  GNPG +TVDNWFKI+K  KDYK+ FCPTVCNFCKV+CRD+G+F ++GK
Sbjct: 126 TKQWFISTCGVEGNPGQKTVDNWFKIDKFEKDYKIRFCPTVCNFCKVICRDVGVFVQDGK 185

Query: 190 RALALSDTPFPVMFKK 205
           R LALSD P  VMFK+
Sbjct: 186 RRLALSDVPLKVMFKR 194

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BLAST of Cmc12g0336351 vs. ExPASy Swiss-Prot
Match: P13087 (Miraculin OS=Synsepalum dulcificum OX=3743 PE=1 SV=3)

HSP 1 Score: 217.6 bits (553), Expect = 1.3e-55
Identity = 113/206 (54.85%), Postives = 139/206 (67.48%), Query Frame = 0


Query: 8   FYFIFILL-ASTELRFSTADASPEAVLDIDGKKLRAGVNYYILPVFRGRGGGLTLGNLQS 67
           F+F+  LL A+     S AD++P  VLDIDG+KLR G NYYI+PV R  GGGLT+     
Sbjct: 11  FFFVSALLAAAANPLLSAADSAPNPVLDIDGEKLRTGTNYYIVPVLRDHGGGLTVSATTP 70

Query: 68  E---ICPVNVVQEQFELMNGFPTTFHPVNPKKGVVRVSTDLNVQFDASTIC--VTSTVWK 127
               +CP  VVQ + E+ +  P  F P NPK+ VVRVSTDLN+ F A   C   +STVW+
Sbjct: 71  NGTFVCPPRVVQTRKEVDHDRPLAFFPENPKEDVVRVSTDLNINFSAFMPCRWTSSTVWR 130

Query: 128 LDKFDESTGQWFVTIGGSRGNPGVETVDNWFKIEKH--GKDYKLVFCPTVCNFCKVMCRD 187
           LDK+DESTGQ+FVTIGG +GNPG ET+ +WFKIE+      YKLVFCPTVC  CKV C D
Sbjct: 131 LDKYDESTGQYFVTIGGVKGNPGPETISSWFKIEEFCGSGFYKLVFCPTVCGSCKVKCGD 190

Query: 188 IGIFF-KNGKRALALSDTPFPVMFKK 205
           +GI+  + G+R LALSD PF   F K
Sbjct: 191 VGIYIDQKGRRRLALSDKPFAFEFNK 216

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BLAST of Cmc12g0336351 vs. ExPASy Swiss-Prot
Match: P32765 (21 kDa seed protein OS=Theobroma cacao OX=3641 GN=ASP PE=2 SV=1)

HSP 1 Score: 170.2 bits (430), Expect = 2.4e-41
Identity = 89/188 (47.34%), Postives = 115/188 (61.17%), Query Frame = 0


Query: 22  FSTADASPEAVLDIDGKKLRAGVNYYILPVFRGR-GGGLTLGNLQSEICPVNVVQEQFEL 81
           F  A+A+   VLD DG +L+ GV YY+L    G  GGGL LG    + CP  VVQ + +L
Sbjct: 21  FGVANAANSPVLDTDGDELQTGVQYYVLSSISGAGGGGLALGRATGQSCPEIVVQRRSDL 80

Query: 82  MNGFPTTFHPVNPKKGVVRVSTDLNVQF--DASTICVTSTVWKLDKFDESTGQWFVTIGG 141
            NG P  F   + K  VVRVSTD+N++F      +C TSTVW+LD +D S G+W+VT  G
Sbjct: 81  DNGTPVIFSNADSKDDVVRVSTDVNIEFVPIRDRLCSTSTVWRLDNYDNSAGKWWVTTDG 140

Query: 142 SRGNPGVETVDNWFKIEKHG-KDYKLVFCPTVCNFCKVMCRDIGIFF-KNGKRALALSDT 201
            +G PG  T+ +WFKIEK G   YK  FCP+VC+ C  +C DIG     +G+  LALSD 
Sbjct: 141 VKGEPGPNTLCSWFKIEKAGVLGYKFRFCPSVCDSCTTLCSDIGRHSDDDGQIRLALSDN 200

Query: 202 PFPVMFKK 205
            +  MFKK
Sbjct: 201 EWAWMFKK 208

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BLAST of Cmc12g0336351 vs. ExPASy Swiss-Prot
Match: Q8RXD5 (Kunitz trypsin inhibitor 4 OS=Arabidopsis thaliana OX=3702 GN=KTI4 PE=2 SV=1)

HSP 1 Score: 151.4 bits (381), Expect = 1.1e-35
Identity = 86/196 (43.88%), Postives = 115/196 (58.67%), Query Frame = 0


Query: 12  FILLASTELRFSTADASPEAVLDIDGKKLRAGVNYYILPVFRGRGGGLTLGNLQSEICPV 71
           +++LA T +  S A     AV+DIDG  +    +YY+LPV RGRGGGLTL     + CP 
Sbjct: 13  YLVLALTAVLASNAYG---AVVDIDGNAM-FHESYYVLPVIRGRGGGLTLAGRGGQPCPY 72

Query: 72  NVVQEQFELMNGFPTTFHPVNPKKGVVRVSTDLNVQFD-ASTICVTSTVWKLDKFDESTG 131
           ++VQE  E+  G P  F     K   V  S +LN++ D  +TIC+ ST W++ +FD    
Sbjct: 73  DIVQESSEVDEGIPVKFSNWRLKVAFVPESQNLNIETDVGATICIQSTYWRVGEFDHERK 132

Query: 132 QWFVTIGGSRGNPGVETVDNWFKIEKHGKD-YKLVFCPTVCNFCKVMCRDIGIFFKN-GK 191
           Q+FV  G      G +++ ++FKIEK G+D YK VFCP  C+     C D+GIF    G 
Sbjct: 133 QYFVVAGPKPEGFGQDSLKSFFKIEKSGEDAYKFVFCPRTCDSGNPKCSDVGIFIDELGV 192

Query: 192 RALALSDTPFPVMFKK 205
           R LALSD PF VMFKK
Sbjct: 193 RRLALSDKPFLVMFKK 204

[Close]

BLAST of Cmc12g0336351 vs. ExPASy Swiss-Prot
Match: P29421 (Alpha-amylase/subtilisin inhibitor OS=Oryza sativa subsp. japonica OX=39947 GN=RASI PE=1 SV=2)

HSP 1 Score: 115.2 bits (287), Expect = 9.0e-25
Identity = 74/196 (37.76%), Postives = 103/196 (52.55%), Query Frame = 0


Query: 13  ILLASTELRFSTADASPEAVLDIDGKKLRAGVNYYILPVFRGRGGGLTLGNLQSEICPVN 72
           ILL+   + FS   A+P  V D +G +L A  +YY+LP   G GGGLT+   +   CP+ 
Sbjct: 9   ILLSLLAISFS-CSAAPPPVYDTEGHELSADGSYYVLPASPGHGGGLTMAP-RVLPCPLL 68

Query: 73  VVQEQFELMNGFPTTFHP----VNPKKGVVRVSTDLNVQFDASTICVTSTVWKLDKFDES 132
           V QE  E   GFP  F P      P+   +RVSTD+ ++F+A+TICV ST W +   +  
Sbjct: 69  VAQETDERRKGFPVRFTPWGGAAAPEDRTIRVSTDVRIRFNAATICVQSTEWHVGD-EPL 128

Query: 133 TGQWFVTIGGSRGNPGVETVDNWFKIEKHGKDYKLVFCPTVCNFCKVMCRDIGIFFKNGK 192
           TG   V  G   G P     +N F++EK+G  YKLV        C+  C+D+G+     +
Sbjct: 129 TGARRVVTGPLIG-PSPSGRENAFRVEKYGGGYKLV-------SCRDSCQDLGVSRDGAR 188

Query: 193 RALALSDTPFPVMFKK 205
             L  S  P  V+FKK
Sbjct: 189 AWLGASQPPHVVVFKK 193

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BLAST of Cmc12g0336351 vs. ExPASy TrEMBL
Match: A0A5D3BIG0 (Miraculin-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G002770 PE=3 SV=1)

HSP 1 Score: 430.3 bits (1105), Expect = 4.7e-117
Identity = 205/205 (100.00%), Postives = 205/205 (100.00%), Query Frame = 0


Query: 1   MTKNFGIFYFIFILLASTELRFSTADASPEAVLDIDGKKLRAGVNYYILPVFRGRGGGLT 60
           MTKNFGIFYFIFILLASTELRFSTADASPEAVLDIDGKKLRAGVNYYILPVFRGRGGGLT
Sbjct: 1   MTKNFGIFYFIFILLASTELRFSTADASPEAVLDIDGKKLRAGVNYYILPVFRGRGGGLT 60

Query: 61  LGNLQSEICPVNVVQEQFELMNGFPTTFHPVNPKKGVVRVSTDLNVQFDASTICVTSTVW 120
           LGNLQSEICPVNVVQEQFELMNGFPTTFHPVNPKKGVVRVSTDLNVQFDASTICVTSTVW
Sbjct: 61  LGNLQSEICPVNVVQEQFELMNGFPTTFHPVNPKKGVVRVSTDLNVQFDASTICVTSTVW 120

Query: 121 KLDKFDESTGQWFVTIGGSRGNPGVETVDNWFKIEKHGKDYKLVFCPTVCNFCKVMCRDI 180
           KLDKFDESTGQWFVTIGGSRGNPGVETVDNWFKIEKHGKDYKLVFCPTVCNFCKVMCRDI
Sbjct: 121 KLDKFDESTGQWFVTIGGSRGNPGVETVDNWFKIEKHGKDYKLVFCPTVCNFCKVMCRDI 180

Query: 181 GIFFKNGKRALALSDTPFPVMFKKV 206
           GIFFKNGKRALALSDTPFPVMFKKV
Sbjct: 181 GIFFKNGKRALALSDTPFPVMFKKV 205

[Close]

BLAST of Cmc12g0336351 vs. ExPASy TrEMBL
Match: A0A1S3ASR3 (miraculin-like OS=Cucumis melo OX=3656 GN=LOC103482596 PE=3 SV=1)

HSP 1 Score: 430.3 bits (1105), Expect = 4.7e-117
Identity = 205/205 (100.00%), Postives = 205/205 (100.00%), Query Frame = 0


Query: 1   MTKNFGIFYFIFILLASTELRFSTADASPEAVLDIDGKKLRAGVNYYILPVFRGRGGGLT 60
           MTKNFGIFYFIFILLASTELRFSTADASPEAVLDIDGKKLRAGVNYYILPVFRGRGGGLT
Sbjct: 1   MTKNFGIFYFIFILLASTELRFSTADASPEAVLDIDGKKLRAGVNYYILPVFRGRGGGLT 60

Query: 61  LGNLQSEICPVNVVQEQFELMNGFPTTFHPVNPKKGVVRVSTDLNVQFDASTICVTSTVW 120
           LGNLQSEICPVNVVQEQFELMNGFPTTFHPVNPKKGVVRVSTDLNVQFDASTICVTSTVW
Sbjct: 61  LGNLQSEICPVNVVQEQFELMNGFPTTFHPVNPKKGVVRVSTDLNVQFDASTICVTSTVW 120

Query: 121 KLDKFDESTGQWFVTIGGSRGNPGVETVDNWFKIEKHGKDYKLVFCPTVCNFCKVMCRDI 180
           KLDKFDESTGQWFVTIGGSRGNPGVETVDNWFKIEKHGKDYKLVFCPTVCNFCKVMCRDI
Sbjct: 121 KLDKFDESTGQWFVTIGGSRGNPGVETVDNWFKIEKHGKDYKLVFCPTVCNFCKVMCRDI 180

Query: 181 GIFFKNGKRALALSDTPFPVMFKKV 206
           GIFFKNGKRALALSDTPFPVMFKKV
Sbjct: 181 GIFFKNGKRALALSDTPFPVMFKKV 205

[Close]

BLAST of Cmc12g0336351 vs. ExPASy TrEMBL
Match: A0A0A0LTW2 (Tumor-related protein OS=Cucumis sativus OX=3659 GN=Csa_1G043200 PE=3 SV=1)

HSP 1 Score: 402.5 bits (1033), Expect = 1.0e-108
Identity = 194/205 (94.63%), Postives = 200/205 (97.56%), Query Frame = 0


Query: 3   KNFGI-FYFIFILLASTEL-RFSTADASPEAVLDIDGKKLRAGVNYYILPVFRGRGGGLT 62
           KNFGI FYF+FILLAST+L RFSTADASPEAVLDIDGKKLRAGVNYYILPVFRGRGGGLT
Sbjct: 2   KNFGILFYFLFILLASTQLIRFSTADASPEAVLDIDGKKLRAGVNYYILPVFRGRGGGLT 61

Query: 63  LGNLQSEICPVNVVQEQFELMNGFPTTFHPVNPKKGVVRVSTDLNVQFDASTICVTSTVW 122
           LGNLQSE CP+NVVQEQ E+MNGFPTTFHPVNPKKGVVRVSTDLNVQF+ASTICVTSTVW
Sbjct: 62  LGNLQSEKCPLNVVQEQLEVMNGFPTTFHPVNPKKGVVRVSTDLNVQFEASTICVTSTVW 121

Query: 123 KLDKFDESTGQWFVTIGGSRGNPGVETVDNWFKIEKHGKDYKLVFCPTVCNFCKVMCRDI 182
           KLDKFDESTGQW VTIGGSRGNPGVETVDNWFKIEKHGKDYKLVFCPTVCNFCKVMCRDI
Sbjct: 122 KLDKFDESTGQWLVTIGGSRGNPGVETVDNWFKIEKHGKDYKLVFCPTVCNFCKVMCRDI 181

Query: 183 GIFFKNGKRALALSDTPFPVMFKKV 206
           GIFFKNG+RALALSDTPFPVMFKKV
Sbjct: 182 GIFFKNGERALALSDTPFPVMFKKV 206

[Close]

BLAST of Cmc12g0336351 vs. ExPASy TrEMBL
Match: A0A6J1KRM6 (kunitz trypsin inhibitor 2 OS=Cucurbita maxima OX=3661 GN=LOC111495838 PE=3 SV=1)

HSP 1 Score: 364.4 bits (934), Expect = 3.1e-97
Identity = 172/203 (84.73%), Postives = 185/203 (91.13%), Query Frame = 0


Query: 3   KNFGIFYFIFILLASTELRFSTADASPEAVLDIDGKKLRAGVNYYILPVFRGRGGGLTLG 62
           KNF +  F+FILLAS E+R S ADASP+AV DIDGKKLRAGVNYYILPVFRGRGGGL LG
Sbjct: 2   KNFALLSFLFILLAS-EVRVSRADASPDAVRDIDGKKLRAGVNYYILPVFRGRGGGLALG 61

Query: 63  NLQSEICPVNVVQEQFELMNGFPTTFHPVNPKKGVVRVSTDLNVQFDASTICVTSTVWKL 122
           NLQS+ CP+NVVQEQFELMNG P  F PVNPKKGVVRVSTDLNVQF+ASTIC TSTVWKL
Sbjct: 62  NLQSDKCPLNVVQEQFELMNGLPAAFLPVNPKKGVVRVSTDLNVQFEASTICATSTVWKL 121

Query: 123 DKFDESTGQWFVTIGGSRGNPGVETVDNWFKIEKHGKDYKLVFCPTVCNFCKVMCRDIGI 182
           DKFDEST QWF+TIGG+RGNPGV+TVDNWFKIEKHG DYK  FCPTVC+FCKVMCRD+GI
Sbjct: 122 DKFDESTKQWFITIGGTRGNPGVKTVDNWFKIEKHGNDYKFKFCPTVCDFCKVMCRDVGI 181

Query: 183 FFKNGKRALALSDTPFPVMFKKV 206
           FFKNGKRALALSDTPFPVMFK+V
Sbjct: 182 FFKNGKRALALSDTPFPVMFKEV 203

[Close]

BLAST of Cmc12g0336351 vs. ExPASy TrEMBL
Match: A0A6J1EAD6 (kunitz trypsin inhibitor 2 OS=Cucurbita moschata OX=3662 GN=LOC111431333 PE=3 SV=1)

HSP 1 Score: 364.4 bits (934), Expect = 3.1e-97
Identity = 172/203 (84.73%), Postives = 185/203 (91.13%), Query Frame = 0


Query: 3   KNFGIFYFIFILLASTELRFSTADASPEAVLDIDGKKLRAGVNYYILPVFRGRGGGLTLG 62
           KNF +  F+FILLAS E+R S ADASP+AV DIDGKKLRAGVNYYILPVFRGRGGGL LG
Sbjct: 2   KNFALLSFLFILLAS-EVRVSRADASPDAVRDIDGKKLRAGVNYYILPVFRGRGGGLALG 61

Query: 63  NLQSEICPVNVVQEQFELMNGFPTTFHPVNPKKGVVRVSTDLNVQFDASTICVTSTVWKL 122
           NLQS+ CP+NVVQEQFELMNG P  F PVNPKKGVVRVSTDLNVQF+ASTIC TSTVWKL
Sbjct: 62  NLQSDKCPLNVVQEQFELMNGLPAAFLPVNPKKGVVRVSTDLNVQFEASTICATSTVWKL 121

Query: 123 DKFDESTGQWFVTIGGSRGNPGVETVDNWFKIEKHGKDYKLVFCPTVCNFCKVMCRDIGI 182
           DKFDEST QWF+TIGG+RGNPGV+TVDNWFKIEKHG DYK  FCPTVC+FCKVMCRD+GI
Sbjct: 122 DKFDESTKQWFITIGGTRGNPGVKTVDNWFKIEKHGNDYKFKFCPTVCDFCKVMCRDVGI 181

Query: 183 FFKNGKRALALSDTPFPVMFKKV 206
           FFKNGKRALALSDTPFPVMFK+V
Sbjct: 182 FFKNGKRALALSDTPFPVMFKEV 203

[Close]

BLAST of Cmc12g0336351 vs. TAIR 10
Match: AT1G17860.1 (Kunitz family trypsin and protease inhibitor protein )

HSP 1 Score: 232.3 bits (591), Expect = 3.6e-61
Identity = 110/196 (56.12%), Postives = 142/196 (72.45%), Query Frame = 0


Query: 10  FIFILLA-STELRFSTADASPEAVLDIDGKKLRAGVNYYILPVFRGRGGGLTLGNLQSEI 69
           +IF+LLA     R  T +A+ E V DI+GK L  GVNYYILPV RGRGGGLT+ NL++E 
Sbjct: 6   YIFLLLAVFISHRGVTTEAAVEPVKDINGKSLLTGVNYYILPVIRGRGGGLTMSNLKTET 65

Query: 70  CPVNVVQEQFELMNGFPTTFHPVNPKKGVVRVSTDLNVQFDASTICVTSTVWKLDKFDES 129
           CP +V+Q+QFE+  G P  F P + K   + VSTD+N++F  ++I      W+L  FDE+
Sbjct: 66  CPTSVIQDQFEVSQGLPVKFSPYD-KSRTIPVSTDVNIKFSPTSI------WELANFDET 125

Query: 130 TGQWFVTIGGSRGNPGVETVDNWFKIEKHGKDYKLVFCPTVCNFCKVMCRDIGIFFKNGK 189
           T QWF++  G  GNPG +TVDNWFKI+K  KDYK+ FCPTVCNFCKV+CRD+G+F ++GK
Sbjct: 126 TKQWFISTCGVEGNPGQKTVDNWFKIDKFEKDYKIRFCPTVCNFCKVICRDVGVFVQDGK 185

Query: 190 RALALSDTPFPVMFKK 205
           R LALSD P  VMFK+
Sbjct: 186 RRLALSDVPLKVMFKR 194

[Close]

BLAST of Cmc12g0336351 vs. TAIR 10
Match: AT1G73260.1 (kunitz trypsin inhibitor 1 )

HSP 1 Score: 151.4 bits (381), Expect = 8.1e-37
Identity = 86/196 (43.88%), Postives = 115/196 (58.67%), Query Frame = 0


Query: 12  FILLASTELRFSTADASPEAVLDIDGKKLRAGVNYYILPVFRGRGGGLTLGNLQSEICPV 71
           +++LA T +  S A     AV+DIDG  +    +YY+LPV RGRGGGLTL     + CP 
Sbjct: 13  YLVLALTAVLASNAYG---AVVDIDGNAM-FHESYYVLPVIRGRGGGLTLAGRGGQPCPY 72

Query: 72  NVVQEQFELMNGFPTTFHPVNPKKGVVRVSTDLNVQFD-ASTICVTSTVWKLDKFDESTG 131
           ++VQE  E+  G P  F     K   V  S +LN++ D  +TIC+ ST W++ +FD    
Sbjct: 73  DIVQESSEVDEGIPVKFSNWRLKVAFVPESQNLNIETDVGATICIQSTYWRVGEFDHERK 132

Query: 132 QWFVTIGGSRGNPGVETVDNWFKIEKHGKD-YKLVFCPTVCNFCKVMCRDIGIFFKN-GK 191
           Q+FV  G      G +++ ++FKIEK G+D YK VFCP  C+     C D+GIF    G 
Sbjct: 133 QYFVVAGPKPEGFGQDSLKSFFKIEKSGEDAYKFVFCPRTCDSGNPKCSDVGIFIDELGV 192

Query: 192 RALALSDTPFPVMFKK 205
           R LALSD PF VMFKK
Sbjct: 193 RRLALSDKPFLVMFKK 204

[Close]

BLAST of Cmc12g0336351 vs. TAIR 10
Match: AT1G73325.1 (Kunitz family trypsin and protease inhibitor protein )

HSP 1 Score: 108.2 bits (269), Expect = 7.8e-24
Identity = 69/190 (36.32%), Postives = 107/190 (56.32%), Query Frame = 0


Query: 23  STADASP-EAVLDIDGKKLRAGVNYYILPVFRGRGGGLTLGNLQ---SEIC-PVNVVQEQ 82
           S ADA+P + VLDI G  +++ V YYI+P   G GGGL   N      ++C  +++VQ  
Sbjct: 22  SAADATPSQVVLDIAGHPVQSNVQYYIIPAKIGTGGGLIPSNRNLSTQDLCLNLDIVQSS 81

Query: 83  FELMNGFPTTFHPVNPKKGVVRVSTDLNVQFDAST-ICVTSTVWKLDKFDESTGQWFVTI 142
              ++G P TF P+N K   V++S  LN++FD++  +C  S VW++D       + FV+I
Sbjct: 82  SPFVSGLPVTFSPLNTKVKHVQLSASLNLEFDSTVWLCPDSKVWRID-HSVQLRKSFVSI 141

Query: 143 GGSRGNPGVETVDNWFKIEKHGKDYKLVFCPTVCNFCKVMCRDIGI-FFKNGKRALALS- 202
           GG +G       ++WF+I++ G  YKL++CP       V C ++ +    +G R L LS 
Sbjct: 142 GGQKGKG-----NSWFQIQEDGDAYKLMYCPI---SSIVACINVSLEIDDHGVRRLVLST 201

Query: 203 DTPFPVMFKK 205
           D  F V F+K
Sbjct: 202 DQSFVVKFQK 202

The following BLAST results are available for this feature:

BLAST of Cmc12g0336351 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_008437058.1	9.6e-117	100.00	PREDICTED: miraculin-like [Cucumis melo] >TYJ99073.1 miraculin-like [Cucumis mel...	[more]
XP_004137534.1	2.2e-108	94.63	kunitz trypsin inhibitor 5 [Cucumis sativus]	[more]
XP_038895203.1	1.6e-103	91.67	kunitz trypsin inhibitor 5-like [Benincasa hispida]	[more]
KAE8652565.1	5.1e-102	95.70	hypothetical protein Csa_014019 [Cucumis sativus]	[more]
XP_022923703.1	6.5e-97	84.73	kunitz trypsin inhibitor 2 [Cucurbita moschata] >XP_023001808.1 kunitz trypsin i...	[more]

BLAST of Cmc12g0336351 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LMU2	5.1e-60	56.12	Kunitz trypsin inhibitor 5 OS=Arabidopsis thaliana OX=3702 GN=KTI5 PE=2 SV=1	[more]
P13087	1.3e-55	54.85	Miraculin OS=Synsepalum dulcificum OX=3743 PE=1 SV=3	[more]
P32765	2.4e-41	47.34	21 kDa seed protein OS=Theobroma cacao OX=3641 GN=ASP PE=2 SV=1	[more]
Q8RXD5	1.1e-35	43.88	Kunitz trypsin inhibitor 4 OS=Arabidopsis thaliana OX=3702 GN=KTI4 PE=2 SV=1	[more]
P29421	9.0e-25	37.76	Alpha-amylase/subtilisin inhibitor OS=Oryza sativa subsp. japonica OX=39947 GN=R...	[more]

BLAST of Cmc12g0336351 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5D3BIG0	4.7e-117	100.00	Miraculin-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G00277...	[more]
A0A1S3ASR3	4.7e-117	100.00	miraculin-like OS=Cucumis melo OX=3656 GN=LOC103482596 PE=3 SV=1	[more]
A0A0A0LTW2	1.0e-108	94.63	Tumor-related protein OS=Cucumis sativus OX=3659 GN=Csa_1G043200 PE=3 SV=1	[more]
A0A6J1KRM6	3.1e-97	84.73	kunitz trypsin inhibitor 2 OS=Cucurbita maxima OX=3661 GN=LOC111495838 PE=3 SV=1	[more]
A0A6J1EAD6	3.1e-97	84.73	kunitz trypsin inhibitor 2 OS=Cucurbita moschata OX=3662 GN=LOC111431333 PE=3 SV...	[more]

BLAST of Cmc12g0336351 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G17860.1	3.6e-61	56.12	Kunitz family trypsin and protease inhibitor protein	[more]
AT1G73260.1	8.1e-37	43.88	kunitz trypsin inhibitor 1	[more]
AT1G73325.1	7.8e-24	36.32	Kunitz family trypsin and protease inhibitor protein	[more]

InterPro

Analysis Name: InterPro Annotations of Melon (Charmono) v1.1
Date Performed: 2022-10-13


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002160	Proteinase inhibitor I3, Kunitz legume	PRINTS	PR00291	KUNITZINHBTR	coord: 151..170 score: 25.29 coord: 174..203 score: 25.03 coord: 31..60 score: 46.34 coord: 69..89 score: 31.19
IPR002160	Proteinase inhibitor I3, Kunitz legume	SMART	SM00452	kul_2	coord: 31..205 e-value: 1.2E-72 score: 257.4
IPR002160	Proteinase inhibitor I3, Kunitz legume	PFAM	PF00197	Kunitz_legume	coord: 32..204 e-value: 1.2E-59 score: 201.1
IPR002160	Proteinase inhibitor I3, Kunitz legume	PANTHER	PTHR33107	KUNITZ TRYPSIN INHIBITOR 2	coord: 11..205
IPR002160	Proteinase inhibitor I3, Kunitz legume	PROSITE	PS00283	SOYBEAN_KUNITZ	coord: 32..48
IPR002160	Proteinase inhibitor I3, Kunitz legume	CDD	cd00178	STI	coord: 31..204 e-value: 1.06932E-56 score: 175.223
None	No IPR available	GENE3D	2.80.10.50		coord: 26..205 e-value: 2.9E-76 score: 257.1
None	No IPR available	PANTHER	PTHR33107:SF5	KUNITZ TRYPSIN INHIBITOR 2	coord: 11..205
IPR011065	Kunitz inhibitor STI-like superfamily	SUPERFAMILY	50386	STI-like	coord: 28..205

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cmc12g0336351.1	Cmc12g0336351.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0010951	negative regulation of endopeptidase activity
molecular_function	GO:0004866	endopeptidase inhibitor activity