Cmc12g0336201 (gene) Melon (Charmono) v1.1

Overview


Name	Cmc12g0336201
Type	gene
Organism	Cucumis melo L. var. cantalupensis cv. Charmono (Melon (Charmono) v1.1)
Description	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein
Location	CMiso1.1chr12: 24825075 .. 24826278 (-)
RNA-Seq Expression	Cmc12g0336201
Synteny	Cmc12g0336201

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCAACAAATATGAGTGATGAACTCCCAGTTGTGAAGTTTTGTTCGGAGAATTTGAAGAGTGGAACGAAGGAATGGGTTTTCACAATGAAGAAAGTTCGAGAAGCTCTTGAAGATTATGGTTGTTTTGTAGCATTATACGATAGCGTTTCGGCAGAAGTAAGTTCGAATATGTTTGATTCTTTAAAAGAATTGTTTGATGATGTTCCATTGGAAAGGAAGCTTCAAAATGTTTCTGAGAAGCCATACCATGGCTACTTTGGTCAAAATCCTCTAATGCCAATCCATGAAAGTATGGGTATCGAACACCCCATTCTCCCCACCAATATCAATTCCTTCACTAATCTCATGTGGCCTTCTCGTGGAAACGACTCTTTCCGGTAATTATTCTTAATTTTGAAACGAAACAAAAACTCCATACTCATGATCTAATAATATGAGCATTATCCTAAACATTTTTGTTATTGTTGCAGTGAAAGTGTGACGACGTATGCAAAATTGGTATCGGAATTGGACGAAAGGGTGAAAAGGATGGTGTTTGAAAGCTATGGGGTTGGGAATGCATTAAAAAGTCATATGGAATCAACAAAATATCTTATGAGAATGATAAAATATAGAGTCCCAAAGGAAAAAGAGATGAATTTGGGAGCTTTTCCTCATACTGATAAGAGCTTTCTCACAATTCTTCATCAAAATGAAGTCAATGGTTTGCAAATCAAAACAAGAGATAACAAATGGATTCAATATCATCCTTTTTCTTCTTCTTCAACCTCATCTTTTATTGTCATGGCTGGAGATGCTTTCTTTGTAAGTTCGTCTCATTTTATTAATTAAATTCTTCATCATCAAATTAAGATTAACATTAAATTAATTAAGCCAAACTAATCATTAATCAACCATTAATTGTTGGCAGGCATGGAGCAATGGGAGGATTTACTCACCACCACATAGAGTGATAATGAGTGGAAATAGAGAGAGATATTCAGTTGGATTGTTTTCATTCAACAATGGAATTGTTCAAATTCCTAAAGAATTGGTTGATGATAAACATCCACAATTGTTTAAACCCTTTGATCATCAAGACTATCTTCGTTTCTATAGCACCGAGAGAGGCCAAAATTCTCCTTGTGCTATCAAAGCTTATTGTGGAGTTGGAAATAGTCTCGTTGATATGGATACTCGAAGTCCTCTTCTCTTGCATTGA

mRNA sequence

ATGGCAACAAATATGAGTGATGAACTCCCAGTTGTGAAGTTTTGTTCGGAGAATTTGAAGAGTGGAACGAAGGAATGGGTTTTCACAATGAAGAAAGTTCGAGAAGCTCTTGAAGATTATGGTTGTTTTGTAGCATTATACGATAGCGTTTCGGCAGAAGTAAGTTCGAATATGTTTGATTCTTTAAAAGAATTGTTTGATGATGTTCCATTGGAAAGGAAGCTTCAAAATGTTTCTGAGAAGCCATACCATGGCTACTTTGGTCAAAATCCTCTAATGCCAATCCATGAAAGTATGGGTATCGAACACCCCATTCTCCCCACCAATATCAATTCCTTCACTAATCTCATGTGGCCTTCTCGTGGAAACGACTCTTTCCGTGAAAGTGTGACGACGTATGCAAAATTGGTATCGGAATTGGACGAAAGGGTGAAAAGGATGGTGTTTGAAAGCTATGGGGTTGGGAATGCATTAAAAAGTCATATGGAATCAACAAAATATCTTATGAGAATGATAAAATATAGAGTCCCAAAGGAAAAAGAGATGAATTTGGGAGCTTTTCCTCATACTGATAAGAGCTTTCTCACAATTCTTCATCAAAATGAAGTCAATGGTTTGCAAATCAAAACAAGAGATAACAAATGGATTCAATATCATCCTTTTTCTTCTTCTTCAACCTCATCTTTTATTGTCATGGCTGGAGATGCTTTCTTTGCATGGAGCAATGGGAGGATTTACTCACCACCACATAGAGTGATAATGAGTGGAAATAGAGAGAGATATTCAGTTGGATTGTTTTCATTCAACAATGGAATTGTTCAAATTCCTAAAGAATTGGTTGATGATAAACATCCACAATTGTTTAAACCCTTTGATCATCAAGACTATCTTCGTTTCTATAGCACCGAGAGAGGCCAAAATTCTCCTTGTGCTATCAAAGCTTATTGTGGAGTTGGAAATAGTCTCGTTGATATGGATACTCGAAGTCCTCTTCTCTTGCATTGA

Coding sequence (CDS)

ATGGCAACAAATATGAGTGATGAACTCCCAGTTGTGAAGTTTTGTTCGGAGAATTTGAAGAGTGGAACGAAGGAATGGGTTTTCACAATGAAGAAAGTTCGAGAAGCTCTTGAAGATTATGGTTGTTTTGTAGCATTATACGATAGCGTTTCGGCAGAAGTAAGTTCGAATATGTTTGATTCTTTAAAAGAATTGTTTGATGATGTTCCATTGGAAAGGAAGCTTCAAAATGTTTCTGAGAAGCCATACCATGGCTACTTTGGTCAAAATCCTCTAATGCCAATCCATGAAAGTATGGGTATCGAACACCCCATTCTCCCCACCAATATCAATTCCTTCACTAATCTCATGTGGCCTTCTCGTGGAAACGACTCTTTCCGTGAAAGTGTGACGACGTATGCAAAATTGGTATCGGAATTGGACGAAAGGGTGAAAAGGATGGTGTTTGAAAGCTATGGGGTTGGGAATGCATTAAAAAGTCATATGGAATCAACAAAATATCTTATGAGAATGATAAAATATAGAGTCCCAAAGGAAAAAGAGATGAATTTGGGAGCTTTTCCTCATACTGATAAGAGCTTTCTCACAATTCTTCATCAAAATGAAGTCAATGGTTTGCAAATCAAAACAAGAGATAACAAATGGATTCAATATCATCCTTTTTCTTCTTCTTCAACCTCATCTTTTATTGTCATGGCTGGAGATGCTTTCTTTGCATGGAGCAATGGGAGGATTTACTCACCACCACATAGAGTGATAATGAGTGGAAATAGAGAGAGATATTCAGTTGGATTGTTTTCATTCAACAATGGAATTGTTCAAATTCCTAAAGAATTGGTTGATGATAAACATCCACAATTGTTTAAACCCTTTGATCATCAAGACTATCTTCGTTTCTATAGCACCGAGAGAGGCCAAAATTCTCCTTGTGCTATCAAAGCTTATTGTGGAGTTGGAAATAGTCTCGTTGATATGGATACTCGAAGTCCTCTTCTCTTGCATTGA

Protein sequence

MATNMSDELPVVKFCSENLKSGTKEWVFTMKKVREALEDYGCFVALYDSVSAEVSSNMFDSLKELFDDVPLERKLQNVSEKPYHGYFGQNPLMPIHESMGIEHPILPTNINSFTNLMWPSRGNDSFRESVTTYAKLVSELDERVKRMVFESYGVGNALKSHMESTKYLMRMIKYRVPKEKEMNLGAFPHTDKSFLTILHQNEVNGLQIKTRDNKWIQYHPFSSSSTSSFIVMAGDAFFAWSNGRIYSPPHRVIMSGNRERYSVGLFSFNNGIVQIPKELVDDKHPQLFKPFDHQDYLRFYSTERGQNSPCAIKAYCGVGNSLVDMDTRSPLLLH

Homology

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BLAST of Cmc12g0336201 vs. NCBI nr
Match: TYJ99087.1 (putative 2-oxoglutarate-dependent dioxygenase AOP1 [Cucumis melo var. makuwa])

HSP 1 Score: 688.0 bits (1774), Expect = 4.2e-194
Identity = 330/334 (98.80%), Postives = 333/334 (99.70%), Query Frame = 0


Query: 1   MATNMSDELPVVKFCSENLKSGTKEWVFTMKKVREALEDYGCFVALYDSVSAEVSSNMFD 60
           MATNMSDELPVVKFCSENLKSGTKEWVFTMKKVREALEDYGCFVALYDSVSAEVSSNMFD
Sbjct: 1   MATNMSDELPVVKFCSENLKSGTKEWVFTMKKVREALEDYGCFVALYDSVSAEVSSNMFD 60

Query: 61  SLKELFDDVPLERKLQNVSEKPYHGYFGQNPLMPIHESMGIEHPILPTNINSFTNLMWPS 120
           SLKELFDDVPLERKLQNVSEKPYHGYFGQNPLMPIHESMGIEHPILPTNINSFTNLMWPS
Sbjct: 61  SLKELFDDVPLERKLQNVSEKPYHGYFGQNPLMPIHESMGIEHPILPTNINSFTNLMWPS 120

Query: 121 RGNDSFRESVTTYAKLVSELDERVKRMVFESYGVGNALKSHMESTKYLMRMIKYRVPKEK 180
           RGNDSFRESVTTYAKLVSELDERVKRMVFESYGVGNALKSHMESTKYLMRMIKYRVPKEK
Sbjct: 121 RGNDSFRESVTTYAKLVSELDERVKRMVFESYGVGNALKSHMESTKYLMRMIKYRVPKEK 180

Query: 181 EMNLGAFPHTDKSFLTILHQNEVNGLQIKTRDNKWIQYHPFSSSSTSSFIVMAGDAFFAW 240
           EMNLGAFPHTDKSFLTILHQNEVNGLQIKTRDNKWIQYHPFSSSSTSSFIVMAGDAFFAW
Sbjct: 181 EMNLGAFPHTDKSFLTILHQNEVNGLQIKTRDNKWIQYHPFSSSSTSSFIVMAGDAFFAW 240

Query: 241 SNGRIYSPPHRVIMSGNRERYSVGLFSFNNGIVQIPKELVDDKHPQLFKPFDHQDYLRFY 300
           SNGRIYSPPHRVIMSGNRERYSVGLFSFNNGIVQIP+ELVDDKHPQLF PFDHQD+LRFY
Sbjct: 241 SNGRIYSPPHRVIMSGNRERYSVGLFSFNNGIVQIPEELVDDKHPQLFNPFDHQDFLRFY 300

Query: 301 STERGQNSPCAIKAYCGVGNSLVDMDTRSPLLLH 335
           STERGQNSPCAIKAYCGVGNSL+DMDTRSPLLLH
Sbjct: 301 STERGQNSPCAIKAYCGVGNSLIDMDTRSPLLLH 334

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BLAST of Cmc12g0336201 vs. NCBI nr
Match: XP_031738134.1 (probable 2-oxoglutarate-dependent dioxygenase AOP1 [Cucumis sativus] >KAE8652571.1 hypothetical protein Csa_014276 [Cucumis sativus])

HSP 1 Score: 660.2 bits (1702), Expect = 9.3e-186
Identity = 318/335 (94.93%), Postives = 327/335 (97.61%), Query Frame = 0


Query: 1   MATNMSDE-LPVVKFCSENLKSGTKEWVFTMKKVREALEDYGCFVALYDSVSAEVSSNMF 60
           MATNMSDE LPVVKFCS+NLKSGT+EWV T KKVREALE+YGCFVALYD+VSAEVSSNMF
Sbjct: 4   MATNMSDEQLPVVKFCSQNLKSGTEEWVSTTKKVREALEEYGCFVALYDTVSAEVSSNMF 63

Query: 61  DSLKELFDDVPLERKLQNVSEKPYHGYFGQNPLMPIHESMGIEHPILPTNINSFTNLMWP 120
           DSLKELFD+VPLERKLQNVSEKPYHGYFGQNPLMPIHESMGIEHPILPTNINSFTNLMWP
Sbjct: 64  DSLKELFDEVPLERKLQNVSEKPYHGYFGQNPLMPIHESMGIEHPILPTNINSFTNLMWP 123

Query: 121 SRGNDSFRESVTTYAKLVSELDERVKRMVFESYGVGNALKSHMESTKYLMRMIKYRVPKE 180
           SRGN SFRE+VT YA LVSELD+RVK MVFESYGVGNAL+SHMESTKYLMRMIKYRVPKE
Sbjct: 124 SRGNHSFRENVTAYANLVSELDKRVKSMVFESYGVGNALRSHMESTKYLMRMIKYRVPKE 183

Query: 181 KEMNLGAFPHTDKSFLTILHQNEVNGLQIKTRDNKWIQYHPFSSSSTSSFIVMAGDAFFA 240
           KEMNLGAFPHTDKSFLTILHQNEVNGLQIKTRDNKWIQYHPFSSSSTSSFIVMAGDAFFA
Sbjct: 184 KEMNLGAFPHTDKSFLTILHQNEVNGLQIKTRDNKWIQYHPFSSSSTSSFIVMAGDAFFA 243

Query: 241 WSNGRIYSPPHRVIMSGNRERYSVGLFSFNNGIVQIPKELVDDKHPQLFKPFDHQDYLRF 300
           WSNGRIYSPPHRVIMSGN+ERYSVGLFSFNNGIVQIPKELVDDKHPQLFKPFDHQDYLRF
Sbjct: 244 WSNGRIYSPPHRVIMSGNKERYSVGLFSFNNGIVQIPKELVDDKHPQLFKPFDHQDYLRF 303

Query: 301 YSTERGQNSPCAIKAYCGVGNSLVDMDTRSPLLLH 335
           YSTERGQNSPCAIKAYCGVGNSLVD DTRSPLLLH
Sbjct: 304 YSTERGQNSPCAIKAYCGVGNSLVDTDTRSPLLLH 338

[Close]

BLAST of Cmc12g0336201 vs. NCBI nr
Match: XP_004151475.3 (probable 2-oxoglutarate-dependent dioxygenase AOP1 [Cucumis sativus] >KAE8652570.1 hypothetical protein Csa_013560 [Cucumis sativus])

HSP 1 Score: 659.8 bits (1701), Expect = 1.2e-185
Identity = 317/335 (94.63%), Postives = 327/335 (97.61%), Query Frame = 0


Query: 1   MATNMSDE-LPVVKFCSENLKSGTKEWVFTMKKVREALEDYGCFVALYDSVSAEVSSNMF 60
           MATNMSDE LPVVKFCS+NLKSGT+EWV T KKVREALE+YGCFVALYD+VSAEVSSNMF
Sbjct: 4   MATNMSDEQLPVVKFCSQNLKSGTEEWVSTTKKVREALEEYGCFVALYDTVSAEVSSNMF 63

Query: 61  DSLKELFDDVPLERKLQNVSEKPYHGYFGQNPLMPIHESMGIEHPILPTNINSFTNLMWP 120
           DSLKELFD+VPLERKLQNVSEKPYHGYFGQNPLMPIHESMGIEHPILPTNINSFTNLMWP
Sbjct: 64  DSLKELFDEVPLERKLQNVSEKPYHGYFGQNPLMPIHESMGIEHPILPTNINSFTNLMWP 123

Query: 121 SRGNDSFRESVTTYAKLVSELDERVKRMVFESYGVGNALKSHMESTKYLMRMIKYRVPKE 180
           SRGN SFRE+VT YA LVSELD+RVK MVFESYGVGNAL+SHMESTKYLMRMIKYRVPKE
Sbjct: 124 SRGNHSFRENVTAYANLVSELDKRVKSMVFESYGVGNALRSHMESTKYLMRMIKYRVPKE 183

Query: 181 KEMNLGAFPHTDKSFLTILHQNEVNGLQIKTRDNKWIQYHPFSSSSTSSFIVMAGDAFFA 240
           KEMNLGAFPHTDKSFLTILHQNEVNGLQIKTRDNKWIQYHPFSSSSTSSFIVMAGDAFFA
Sbjct: 184 KEMNLGAFPHTDKSFLTILHQNEVNGLQIKTRDNKWIQYHPFSSSSTSSFIVMAGDAFFA 243

Query: 241 WSNGRIYSPPHRVIMSGNRERYSVGLFSFNNGIVQIPKELVDDKHPQLFKPFDHQDYLRF 300
           WSNGRIYSPPHRVIMSGN+ERYSVGLFSFNNGIVQIPKELVDDKHPQLFKPFDHQDYLRF
Sbjct: 244 WSNGRIYSPPHRVIMSGNKERYSVGLFSFNNGIVQIPKELVDDKHPQLFKPFDHQDYLRF 303

Query: 301 YSTERGQNSPCAIKAYCGVGNSLVDMDTRSPLLLH 335
           YSTERGQNSPCAIKAYCGVGNSL+D DTRSPLLLH
Sbjct: 304 YSTERGQNSPCAIKAYCGVGNSLIDTDTRSPLLLH 338

[Close]

BLAST of Cmc12g0336201 vs. NCBI nr
Match: XP_038895877.1 (probable 2-oxoglutarate-dependent dioxygenase AOP1 [Benincasa hispida])

HSP 1 Score: 607.1 bits (1564), Expect = 9.4e-170
Identity = 289/330 (87.58%), Postives = 308/330 (93.33%), Query Frame = 0


Query: 5   MSDELPVVKFCSENLKSGTKEWVFTMKKVREALEDYGCFVALYDSVSAEVSSNMFDSLKE 64
           M  E+P+VKFCSENLKSG KEWV T +KVREALE+YGCFVALYD+VS +VSSNMF SLK+
Sbjct: 1   MGGEIPIVKFCSENLKSGRKEWVLTREKVREALEEYGCFVALYDTVSMDVSSNMFHSLKQ 60

Query: 65  LFDDVPLERKLQNVSEKPYHGYFGQNPLMPIHESMGIEHPILPTNINSFTNLMWPSRGND 124
           LF DVPLERK+QNVSEKPYHGYFGQNPLMPIHESMGIEHPILPTNI+SFTNLMWPS GN 
Sbjct: 61  LF-DVPLERKIQNVSEKPYHGYFGQNPLMPIHESMGIEHPILPTNIHSFTNLMWPSHGNR 120

Query: 125 SFRESVTTYAKLVSELDERVKRMVFESYGVGNALKSHMESTKYLMRMIKYRVPKEKEMNL 184
            F E+VT YAKLVSELDERVK MVFESYGVG  LK+HMESTKYLMRMIKYRVPKEKEMNL
Sbjct: 121 PFCENVTLYAKLVSELDERVKSMVFESYGVGKVLKTHMESTKYLMRMIKYRVPKEKEMNL 180

Query: 185 GAFPHTDKSFLTILHQNEVNGLQIKTRDNKWIQYHPFSSSSTSSFIVMAGDAFFAWSNGR 244
           GAFPHTDKSFLTILHQNEVNGLQIKTRDNKW+QYHP SSSSTSSFIVMAGDAFFAWSNGR
Sbjct: 181 GAFPHTDKSFLTILHQNEVNGLQIKTRDNKWLQYHPPSSSSTSSFIVMAGDAFFAWSNGR 240

Query: 245 IYSPPHRVIMSGNRERYSVGLFSFNNGIVQIPKELVDDKHPQLFKPFDHQDYLRFYSTER 304
           IYSPPHRV++S N+ERYSVGLFSFN+GI+QIPKELVDDKHPQ F PFDHQDYLRFY+TER
Sbjct: 241 IYSPPHRVLVSANKERYSVGLFSFNDGIIQIPKELVDDKHPQQFNPFDHQDYLRFYTTER 300

Query: 305 GQNSPCAIKAYCGVGNSLVDMDTRSPLLLH 335
           GQNSPCAIKAYCGVGNSLVD D+RS LLLH
Sbjct: 301 GQNSPCAIKAYCGVGNSLVDTDSRSTLLLH 329

[Close]

BLAST of Cmc12g0336201 vs. NCBI nr
Match: XP_038893580.1 (probable 2-oxoglutarate-dependent dioxygenase AOP1 [Benincasa hispida])

HSP 1 Score: 604.4 bits (1557), Expect = 6.1e-169
Identity = 287/327 (87.77%), Postives = 307/327 (93.88%), Query Frame = 0


Query: 8   ELPVVKFCSENLKSGTKEWVFTMKKVREALEDYGCFVALYDSVSAEVSSNMFDSLKELFD 67
           ++P+VKFCSENLKSG KEWV T +KVREALE+YGCFVALYD+VS +VSSNMF SLK+LF 
Sbjct: 5   QIPIVKFCSENLKSGRKEWVLTREKVREALEEYGCFVALYDTVSMDVSSNMFHSLKQLF- 64

Query: 68  DVPLERKLQNVSEKPYHGYFGQNPLMPIHESMGIEHPILPTNINSFTNLMWPSRGNDSFR 127
           DVPLERK+QNVSEKPYHGYFGQNPLMPIHESMGIEHPILPTNI+SFTNLMWPS GN  F 
Sbjct: 65  DVPLERKIQNVSEKPYHGYFGQNPLMPIHESMGIEHPILPTNIHSFTNLMWPSHGNRPFC 124

Query: 128 ESVTTYAKLVSELDERVKRMVFESYGVGNALKSHMESTKYLMRMIKYRVPKEKEMNLGAF 187
           E+VT YAKLVSELDERVK MVFESYGVG  LK+HMESTKYLMRMIKYRVPKEKEMNLGAF
Sbjct: 125 ENVTLYAKLVSELDERVKSMVFESYGVGKVLKTHMESTKYLMRMIKYRVPKEKEMNLGAF 184

Query: 188 PHTDKSFLTILHQNEVNGLQIKTRDNKWIQYHPFSSSSTSSFIVMAGDAFFAWSNGRIYS 247
           PHTDKSFLTILHQNEVNGLQIKTRDNKW+QYHP SSSSTSSFIVMAGDAFFAWSNGRIYS
Sbjct: 185 PHTDKSFLTILHQNEVNGLQIKTRDNKWLQYHPPSSSSTSSFIVMAGDAFFAWSNGRIYS 244

Query: 248 PPHRVIMSGNRERYSVGLFSFNNGIVQIPKELVDDKHPQLFKPFDHQDYLRFYSTERGQN 307
           PPHRV++S N+ERYSVGLFSFN+GI+QIPKELVDDKHPQ F PFDHQDYLRFY+TERGQN
Sbjct: 245 PPHRVLVSANKERYSVGLFSFNDGIIQIPKELVDDKHPQQFNPFDHQDYLRFYTTERGQN 304

Query: 308 SPCAIKAYCGVGNSLVDMDTRSPLLLH 335
           SPCAIKAYCGVGNSLVD D+RS LLLH
Sbjct: 305 SPCAIKAYCGVGNSLVDTDSRSTLLLH 330

[Close]

BLAST of Cmc12g0336201 vs. ExPASy Swiss-Prot
Match: Q945B6 (Probable 2-oxoglutarate-dependent dioxygenase AOP1.2 OS=Arabidopsis thaliana OX=3702 GN=AOP1.2 PE=2 SV=1)

HSP 1 Score: 282.3 bits (721), Expect = 6.9e-75
Identity = 136/319 (42.63%), Postives = 211/319 (66.14%), Query Frame = 0


Query: 4   NMSDELPVVKFCSENLKSGTKEWVFTMKKVREALEDYGCFVALYDSVSAEVSSNMFDSLK 63
           ++S +LPV+ F  +NLK G+ +W      V +ALEDYGCF A +D +S E++ ++F++++
Sbjct: 9   SVSFQLPVIDFSDQNLKPGSSKWDEVKADVLKALEDYGCFEAFFDKLSVELNRSVFEAME 68

Query: 64  ELFDDVPLERKLQNVSEKPYHGYFGQNPLMPIHESMGIEHPILPTNINSFTNLMWPSRGN 123
           +LF ++P+  K +NVS KP+HGY   N    ++ES+GI+   +   +N FT  +WP  GN
Sbjct: 69  DLF-ELPIPTKQRNVSSKPFHGYLCHN----LYESLGIDDANVLEKVNDFTQQLWPDHGN 128

Query: 124 DSFRESVTTYAKLVSELDERVKRMVFESYGVGNALKSHMESTKYLMRMIKYRVP-----K 183
            S  E++  +++ + ELD  V+RM+ ES+G+ N +  H+ ST YL R++KY  P      
Sbjct: 129 KSISETIHLFSEQLVELDLMVRRMIMESFGIENYIDEHLNSTYYLTRLMKYTSPPDDDDD 188

Query: 184 EKEMNLGAFPHTDKSFLTILHQNEVNGLQIKTRDNKWIQYHPFSSSSTSSFIVMAGDAFF 243
           ++E  LG   HTDK+ +TILHQ +V+GL++KT+D+KWI+  P    S  S +VM GD+  
Sbjct: 189 DEETKLGLRSHTDKNIITILHQYQVDGLEVKTKDDKWIKVKP----SQDSVLVMVGDSLC 248

Query: 244 AWSNGRIYSPPHRVIMSGNRERYSVGLFSF--NNGIVQIPKELVDDKHPQLFKPFDHQDY 303
           A  NGR++SP HRVIM+G + RYS GLFS      I+  P+ELVD +HP++FKPF++ D+
Sbjct: 249 ALLNGRLHSPYHRVIMTGKKTRYSTGLFSIPKTGVIIDSPEELVDKEHPRIFKPFEYTDF 308

Query: 304 LRFYSTERGQNSPCAIKAY 316
           L F+ TE G+ +  A+ A+
Sbjct: 309 LHFFQTEAGRIAQSALHAF 318

[Close]

BLAST of Cmc12g0336201 vs. ExPASy Swiss-Prot
Match: Q9ZTA3 (Probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Arabidopsis thaliana OX=3702 GN=AOP1 PE=2 SV=1)

HSP 1 Score: 280.4 bits (716), Expect = 2.6e-74
Identity = 136/320 (42.50%), Postives = 210/320 (65.62%), Query Frame = 0


Query: 4   NMSDELPVVKFCSENLKSGTKEWVFTMKKVREALEDYGCFVALYDSVSAEVSSNMFDSLK 63
           ++S +LPV+ F  +NLK G+ +W      V +ALEDYGCF A +D +S E++ ++F++++
Sbjct: 9   SVSFQLPVIDFSDQNLKPGSSKWDEVTADVLKALEDYGCFEASFDKLSVELNRSVFEAME 68

Query: 64  ELFDDVPLERKLQNVSEKPYHGYFGQNPLMPIHESMGIEHPILPTNINSFTNLMWPSRGN 123
           +LF ++P+  K +NVS KP+HGY   N    ++ES+GI    +   +N FT  +WP  GN
Sbjct: 69  DLF-ELPIPTKQRNVSSKPFHGYLCHN----LYESLGINDANVLEKVNDFTQQLWPDHGN 128

Query: 124 DSFRESVTTYAKLVSELDERVKRMVFESYGVGNALKSHMESTKYLMRMIKYRVP------ 183
            S  E++  +++ + ELD  V+RM+ ES+G+ N +  H+ ST YL R++KY  P      
Sbjct: 129 KSISETIHLFSEQLVELDLMVRRMIMESFGIENYIDEHLNSTYYLTRLMKYTSPPDDDDD 188

Query: 184 KEKEMNLGAFPHTDKSFLTILHQNEVNGLQIKTRDNKWIQYHPFSSSSTSSFIVMAGDAF 243
            ++E  LG   HTDK+ +TILHQ +V+GL++KT+D+KWI+  P    S  S +VM GD+ 
Sbjct: 189 DDEETKLGLRSHTDKNIITILHQYQVDGLEVKTKDDKWIKVKP----SQDSVLVMVGDSL 248

Query: 244 FAWSNGRIYSPPHRVIMSGNRERYSVGLFSF--NNGIVQIPKELVDDKHPQLFKPFDHQD 303
            A  NGR++SP HRVIM+G + RYS GLFS      I+  P+ELVD +HP++FKPF++ D
Sbjct: 249 CALLNGRLHSPYHRVIMTGKKTRYSTGLFSIPKTGVIIDSPEELVDKEHPRIFKPFEYTD 308

Query: 304 YLRFYSTERGQNSPCAIKAY 316
           +L F+ TE G+ +  A+ A+
Sbjct: 309 FLHFFQTEAGRIAQSALHAF 319

[Close]

BLAST of Cmc12g0336201 vs. ExPASy Swiss-Prot
Match: Q944Z5 (Probable 2-oxoglutarate-dependent dioxygenase AOP1 (Fragment) OS=Arabidopsis thaliana OX=3702 GN=AOP1 PE=2 SV=1)

HSP 1 Score: 271.6 bits (693), Expect = 1.2e-71
Identity = 132/309 (42.72%), Postives = 203/309 (65.70%), Query Frame = 0


Query: 4   NMSDELPVVKFCSENLKSGTKEWVFTMKKVREALEDYGCFVALYDSVSAEVSSNMFDSLK 63
           ++S +LPV+ F  +NLK G+ +W      V +ALEDYGCF A +D +S E++ ++F++++
Sbjct: 2   SVSFQLPVIDFSDQNLKPGSSKWDEVTADVLKALEDYGCFEASFDKLSVELNRSVFEAME 61

Query: 64  ELFDDVPLERKLQNVSEKPYHGYFGQNPLMPIHESMGIEHPILPTNINSFTNLMWPSRGN 123
           +LF ++P+  K +NVS K +HGY   N    ++ES+GI    +   +N FT  +WP  GN
Sbjct: 62  DLF-ELPIPTKQRNVSSKLFHGYLCHN----LYESLGINDANVLEKVNDFTQQLWPDHGN 121

Query: 124 DSFRESVTTYAKLVSELDERVKRMVFESYGVGNALKSHMESTKYLMRMIKYRVP----KE 183
            S  E++  +++ + ELD  V+RM+ ES+G+   +  H+ ST YL R++KY  P     +
Sbjct: 122 KSISETIHLFSEQLVELDLMVRRMIMESFGIEKYIDEHLNSTYYLTRLMKYTSPPDDDDD 181

Query: 184 KEMNLGAFPHTDKSFLTILHQNEVNGLQIKTRDNKWIQYHPFSSSSTSSFIVMAGDAFFA 243
           +E  LG   HTDK+ +TILHQ +V+GL++KT+D+KWI+  P    S  S +VM GD+  A
Sbjct: 182 EETKLGLRSHTDKNIITILHQYQVDGLEVKTKDDKWIKVKP----SQDSVLVMVGDSLCA 241

Query: 244 WSNGRIYSPPHRVIMSGNRERYSVGLFSF--NNGIVQIPKELVDDKHPQLFKPFDHQDYL 303
             NGR++SP HRVIM+G + RYS GLFS      I+  P+ELVD +HP++FKPF++ D+L
Sbjct: 242 LLNGRLHSPYHRVIMTGKKTRYSTGLFSIPKTGVIIDSPEELVDKEHPRIFKPFEYTDFL 301

Query: 304 RFYSTERGQ 307
            F+ TE G+
Sbjct: 302 HFFQTEAGR 301

[Close]

BLAST of Cmc12g0336201 vs. ExPASy Swiss-Prot
Match: A0A0N9HQ36 (Deoxypodophyllotoxin synthase OS=Sinopodophyllum hexandrum OX=93608 GN=2-ODD PE=1 SV=1)

HSP 1 Score: 235.0 bits (598), Expect = 1.3e-60
Identity = 123/307 (40.07%), Postives = 181/307 (58.96%), Query Frame = 0


Query: 9   LPVVKFCSENLKSGTKEWVFTMKKVREALEDYGCFVALYDSVSAEVSSNMFDSLKELFDD 68
           LPV+    +NLK GT  W     +VREALE+YGCF A+ D+VS E+   + +   EL  +
Sbjct: 9   LPVIDLSMKNLKPGTTSWNSVRTQVREALEEYGCFEAVIDAVSPELQKAVCNKGHELL-N 68

Query: 69  VPLERKLQNVSEKPYHGYFGQNPLMPIHESMGIEHPILPTNINSFTNLMWPSRGNDSFRE 128
           +PLE K+ N ++  Y G+     +  ++E MG+        +  FTNLMWP  GN  F E
Sbjct: 69  LPLETKMLNGNKPEYDGF---TSIPNLNEGMGVGRITDLEKVERFTNLMWP-EGNKDFCE 128

Query: 129 SVTTYAKLVSELDERVKRMVFESYGVGNALKSHMESTKYLMRMIKYRVPKEKEMNLGAFP 188
           +V +Y K ++E+D  +K MVFES+G+     S  EST YL+  ++Y+ P +   +     
Sbjct: 129 TVYSYGKRMAEVDHILKMMVFESFGMEKHFDSFCESTNYLLHFMRYQQPGKDGRSPALSL 188

Query: 189 HTDKSFLTILHQNEVNGLQIKTRDNKWIQYHPFSSSSTSSFIVMAGDAFFAWSNGRIYSP 248
           H DKS LTI++QN+V GL+ +T+D +WI        +  + IV+ GD F AWSNGR++SP
Sbjct: 189 HKDKSILTIVNQNDVKGLEFETKDGEWI------LPTADNHIVLLGDCFMAWSNGRLHSP 248

Query: 249 PHRVIMSGNRERYSVGLFSFNNGIVQIPKELVDDKHPQLFKPFDHQDYLRFYSTERGQNS 308
            HRV +  N+ R S   FSF   I++ P ELVD++HP LF PF+  + L +  T+ G  +
Sbjct: 249 LHRVTLVANQARLSTSSFSFPKDIIETPAELVDEEHPLLFNPFEITELLAYCFTKEGAKA 304

Query: 309 PCAIKAY 316
            C +K Y
Sbjct: 309 VCDLKQY 304

[Close]

BLAST of Cmc12g0336201 vs. ExPASy Swiss-Prot
Match: Q945B5 (2-oxoglutarate-dependent dioxygenase AOP2 OS=Arabidopsis thaliana OX=3702 GN=AOP2 PE=2 SV=1)

HSP 1 Score: 216.5 bits (550), Expect = 4.7e-55
Identity = 130/434 (29.95%), Postives = 203/434 (46.77%), Query Frame = 0


Query: 8   ELPVVKFCSENLKSGTKEWVFTMKKVREALEDYGCFVALYDSVSAEVSSNMFDSLKELFD 67
           +LP++    + L+ G+ +W      VR+ALED+GCF A YD VS E+  ++  +++ELF 
Sbjct: 7   QLPLINLADKTLEPGSSKWAEVRSDVRKALEDFGCFEASYDKVSLELQESIMKTMEELF- 66

Query: 68  DVPLERKLQNVSEKPYHGYFGQNPLMPIHESMGIEHPILPTNINSFTNLMWP-SRGNDSF 127
            +P+E K +NV  KPY GY   N L    ES+GI +  +  N+N FT  +WP   GN++ 
Sbjct: 67  ALPVETKQRNVCPKPYVGYLNHNNL---SESLGISNANILENVNEFTQQLWPDGDGNENI 126

Query: 128 RESVTTYAKLVSELDERVKRMVFESYGVGNALKSHMESTKYLMRMIKYRVPKE------- 187
            +++  +A+ + E+D  V+RMV ES+G+   +  H++ST+Y MR++KY  P E       
Sbjct: 127 SKTIQLFAEKLVEIDVMVRRMVMESFGIEKYIDDHLKSTEYRMRLMKYIAPPEGDANTTV 186

Query: 188 ------------------------------------------------------------ 247
                                                                       
Sbjct: 187 DDYADLLAKLNIDGVEPNVGVKVNADISDDVNANPSVNAGVGANVNADTGVNDNLNVDAE 246

Query: 248 -------------------------------------------------KEMNLGAFPHT 307
                                                            +E  LG   HT
Sbjct: 247 ANGDANIAVGGGVNANTDLGVGVNVNSNVAVNAKTGATSGDDVEANDDNEEKKLGLPCHT 306

Query: 308 DKSFLTILHQNEVNGLQIKTRDNKWIQYHPFSSSSTSSFIVMAGDAFFAWSNGRIYSPPH 322
           DK+  T+L Q+E+ GL++KT+D KWI+  P    S ++FIV+AGD+  A  NGRI +P H
Sbjct: 307 DKNLFTVLFQHEIEGLEVKTKDEKWIRVKP----SPNTFIVIAGDSLCALMNGRIRAPYH 366

[Close]

BLAST of Cmc12g0336201 vs. ExPASy TrEMBL
Match: A0A5D3BIZ7 (Putative 2-oxoglutarate-dependent dioxygenase AOP1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G002910 PE=3 SV=1)

HSP 1 Score: 688.0 bits (1774), Expect = 2.0e-194
Identity = 330/334 (98.80%), Postives = 333/334 (99.70%), Query Frame = 0


Query: 1   MATNMSDELPVVKFCSENLKSGTKEWVFTMKKVREALEDYGCFVALYDSVSAEVSSNMFD 60
           MATNMSDELPVVKFCSENLKSGTKEWVFTMKKVREALEDYGCFVALYDSVSAEVSSNMFD
Sbjct: 1   MATNMSDELPVVKFCSENLKSGTKEWVFTMKKVREALEDYGCFVALYDSVSAEVSSNMFD 60

Query: 61  SLKELFDDVPLERKLQNVSEKPYHGYFGQNPLMPIHESMGIEHPILPTNINSFTNLMWPS 120
           SLKELFDDVPLERKLQNVSEKPYHGYFGQNPLMPIHESMGIEHPILPTNINSFTNLMWPS
Sbjct: 61  SLKELFDDVPLERKLQNVSEKPYHGYFGQNPLMPIHESMGIEHPILPTNINSFTNLMWPS 120

Query: 121 RGNDSFRESVTTYAKLVSELDERVKRMVFESYGVGNALKSHMESTKYLMRMIKYRVPKEK 180
           RGNDSFRESVTTYAKLVSELDERVKRMVFESYGVGNALKSHMESTKYLMRMIKYRVPKEK
Sbjct: 121 RGNDSFRESVTTYAKLVSELDERVKRMVFESYGVGNALKSHMESTKYLMRMIKYRVPKEK 180

Query: 181 EMNLGAFPHTDKSFLTILHQNEVNGLQIKTRDNKWIQYHPFSSSSTSSFIVMAGDAFFAW 240
           EMNLGAFPHTDKSFLTILHQNEVNGLQIKTRDNKWIQYHPFSSSSTSSFIVMAGDAFFAW
Sbjct: 181 EMNLGAFPHTDKSFLTILHQNEVNGLQIKTRDNKWIQYHPFSSSSTSSFIVMAGDAFFAW 240

Query: 241 SNGRIYSPPHRVIMSGNRERYSVGLFSFNNGIVQIPKELVDDKHPQLFKPFDHQDYLRFY 300
           SNGRIYSPPHRVIMSGNRERYSVGLFSFNNGIVQIP+ELVDDKHPQLF PFDHQD+LRFY
Sbjct: 241 SNGRIYSPPHRVIMSGNRERYSVGLFSFNNGIVQIPEELVDDKHPQLFNPFDHQDFLRFY 300

Query: 301 STERGQNSPCAIKAYCGVGNSLVDMDTRSPLLLH 335
           STERGQNSPCAIKAYCGVGNSL+DMDTRSPLLLH
Sbjct: 301 STERGQNSPCAIKAYCGVGNSLIDMDTRSPLLLH 334

[Close]

BLAST of Cmc12g0336201 vs. ExPASy TrEMBL
Match: A0A6J1C712 (probable 2-oxoglutarate-dependent dioxygenase AOP1 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111008991 PE=3 SV=1)

HSP 1 Score: 538.1 bits (1385), Expect = 2.6e-149
Identity = 262/328 (79.88%), Postives = 291/328 (88.72%), Query Frame = 0


Query: 8   ELPVVKFCSENLKSGTKEWVFTMKKVREALEDYGCFVALYDSVSAEVSSNMFDSLKELFD 67
           +LP+VKF  ENL+S    WV T +KVREALE+YGCFVALYDSVS E+SSNMFDSLKELF 
Sbjct: 5   QLPLVKFSEENLRS----WVSTREKVREALEEYGCFVALYDSVSMELSSNMFDSLKELF- 64

Query: 68  DVPLERKLQNVSEKPYHGYFGQNPLMPIHESMGIEHPILPTNINSFTNLMWPSRGNDSFR 127
           +VPLERKL+NVSEKPYHGYFGQNPLMPIHESMGIE P L +   SFTNLMWPS GN SF 
Sbjct: 65  EVPLERKLKNVSEKPYHGYFGQNPLMPIHESMGIEDPNLLSKTQSFTNLMWPSNGNKSFC 124

Query: 128 ESVTTYAKLVSELDERVKRMVFESYGVGNALKSHMESTKYLMRMIKYRVPKEKEMNLGAF 187
           E+V +YA+ ++ELDE VK+MVFESYGV   L+SHM+ST+YL+RMIKYRVPK+ EMN+GAF
Sbjct: 125 ENVNSYARAIAELDETVKKMVFESYGVRKHLESHMKSTRYLLRMIKYRVPKQNEMNMGAF 184

Query: 188 PHTDKSFLTILHQNEVNGLQIKTRDNKWIQYHP-FSSSSTSSFIVMAGDAFFAWSNGRIY 247
           PHTDKSFLTILHQN+VNGLQIKT+ NKW+QYHP  SSSSTSSFIVMAGDAFFAWSNGRIY
Sbjct: 185 PHTDKSFLTILHQNQVNGLQIKTKHNKWLQYHPSSSSSSTSSFIVMAGDAFFAWSNGRIY 244

Query: 248 SPPHRVIMSGNRERYSVGLFSFNNGIVQIPKELVDDKHPQLFKPFDHQDYLRFYSTERGQ 307
           SPPH+VIMSG RERYSVGLFSFN+GIVQIPKELVD+ HPQ FKPFDHQDYLRFYSTERGQ
Sbjct: 245 SPPHKVIMSGKRERYSVGLFSFNDGIVQIPKELVDENHPQQFKPFDHQDYLRFYSTERGQ 304

Query: 308 NSPCAIKAYCGVGNSLVDMDTRSPLLLH 335
           NSPCAIKAYCG+GN LV  DTR  +LLH
Sbjct: 305 NSPCAIKAYCGIGNGLV--DTRGCILLH 325

[Close]

BLAST of Cmc12g0336201 vs. ExPASy TrEMBL
Match: A0A6J1EAT7 (probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Cucurbita moschata OX=3662 GN=LOC111431473 PE=3 SV=1)

HSP 1 Score: 503.8 bits (1296), Expect = 5.4e-139
Identity = 243/329 (73.86%), Postives = 282/329 (85.71%), Query Frame = 0


Query: 5   MSDELPVVKFCSENLKSGTKEWVFTMKKVREALEDYGCFVALYDSVSAEVSS-NMFDSLK 64
           M+ E+PVV+F S NL+SG ++W  T +KVREALE+YGCFVALYDSVS E SS  M +++K
Sbjct: 1   MAGEIPVVRFSSHNLQSGGRKWGSTREKVREALEEYGCFVALYDSVSMEASSLKMMEAMK 60

Query: 65  ELFDDVPLERKLQNVSEKPYHGYFGQNPLMPIHESMGIEHPILPTNINSFTNLMWPSRGN 124
           E+F +VPLERK+QNVSEKP+HGYFGQNPLMPIHESMGIE P+LP+NI SF+NLMWPSR N
Sbjct: 61  EVF-EVPLERKVQNVSEKPFHGYFGQNPLMPIHESMGIEDPLLPSNIQSFSNLMWPSRPN 120

Query: 125 DSFRESVTTYAKLVSELDERVKRMVFESYGVGNALKSHMESTKYLMRMIKYRVPKEKEMN 184
             F E++ +YA+LVS+L++ VK+MVFESYG+ +   SH +STKYL+RMIKYRVP   EMN
Sbjct: 121 PLFCENLKSYAELVSQLEQTVKKMVFESYGLKSLFLSHTQSTKYLLRMIKYRVPHRNEMN 180

Query: 185 LGAFPHTDKSFLTILHQNEVNGLQIKTRDNKWIQYHPFSS-SSTSSFIVMAGDAFFAWSN 244
           LGAFPHTDKSFLTILHQN VNGL IKTRDN+W++Y P S+ SS  SFIVMAGDAFFAWSN
Sbjct: 181 LGAFPHTDKSFLTILHQNSVNGLHIKTRDNRWVEYQPPSAESSAKSFIVMAGDAFFAWSN 240

Query: 245 GRIYSPPHRVIMSGNRERYSVGLFSFNNGIVQIPKELVDDKHPQLFKPFDHQDYLRFYST 304
           GRIYS PHRV MSGNRERYSVGLFSFN+GIVQIPKELVDDKHPQ FK FDHQDYLRFYST
Sbjct: 241 GRIYSAPHRVTMSGNRERYSVGLFSFNDGIVQIPKELVDDKHPQQFKAFDHQDYLRFYST 300

Query: 305 ERGQNSPCAIKAYCGVGNSLVDMDTRSPL 332
           E+GQ+SP AIKAYCGV N L+D  T  P+
Sbjct: 301 EKGQSSPSAIKAYCGVANGLLDSRTCIPM 328

[Close]

BLAST of Cmc12g0336201 vs. ExPASy TrEMBL
Match: A0A6J1E7C8 (probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Cucurbita moschata OX=3662 GN=LOC111431472 PE=3 SV=1)

HSP 1 Score: 499.2 bits (1284), Expect = 1.3e-137
Identity = 241/329 (73.25%), Postives = 281/329 (85.41%), Query Frame = 0


Query: 5   MSDELPVVKFCSENLKSGTKEWVFTMKKVREALEDYGCFVALYDSVSAEVSS-NMFDSLK 64
           M+ ELPVV+F S NL+SG ++W  T +KVREALE+YGCFVAL DSVS E SS  M ++++
Sbjct: 1   MAGELPVVRFSSHNLQSGGRKWGSTREKVREALEEYGCFVALCDSVSMEASSLKMMEAME 60

Query: 65  ELFDDVPLERKLQNVSEKPYHGYFGQNPLMPIHESMGIEHPILPTNINSFTNLMWPSRGN 124
           E+F +VPLERK+QNVSEKP+HGYFGQNPLMPIHESMGIE P+LP+NI SF+NLMWPSR N
Sbjct: 61  EVF-EVPLERKVQNVSEKPFHGYFGQNPLMPIHESMGIEDPLLPSNIQSFSNLMWPSRPN 120

Query: 125 DSFRESVTTYAKLVSELDERVKRMVFESYGVGNALKSHMESTKYLMRMIKYRVPKEKEMN 184
             F E++ +YA+LVS+L++ VK+MVFESYG+ +   SH +STKYL+RMIKYRVP   EMN
Sbjct: 121 PLFCENLKSYAELVSQLEQTVKKMVFESYGLKSLFLSHTQSTKYLLRMIKYRVPHRNEMN 180

Query: 185 LGAFPHTDKSFLTILHQNEVNGLQIKTRDNKWIQYHPFSS-SSTSSFIVMAGDAFFAWSN 244
           LGAFPHTDKSFLTILHQN VNGL IKTRDN+W++Y P S+ SS  SFI+MAGDAFFAWSN
Sbjct: 181 LGAFPHTDKSFLTILHQNSVNGLHIKTRDNRWVEYQPPSAESSAKSFIIMAGDAFFAWSN 240

Query: 245 GRIYSPPHRVIMSGNRERYSVGLFSFNNGIVQIPKELVDDKHPQLFKPFDHQDYLRFYST 304
           GRIYS PHRV MSGNRERYSVGLFSFN+GIVQIPKELVDDKHPQ FK FDHQDYLRFYST
Sbjct: 241 GRIYSAPHRVTMSGNRERYSVGLFSFNDGIVQIPKELVDDKHPQQFKAFDHQDYLRFYST 300

Query: 305 ERGQNSPCAIKAYCGVGNSLVDMDTRSPL 332
           E+GQ+SP AIKAYCGV N L+D  T  P+
Sbjct: 301 EKGQSSPSAIKAYCGVANGLLDSRTCIPM 328

[Close]

BLAST of Cmc12g0336201 vs. ExPASy TrEMBL
Match: A0A6J1KFF7 (probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Cucurbita maxima OX=3661 GN=LOC111495326 PE=3 SV=1)

HSP 1 Score: 495.0 bits (1273), Expect = 2.5e-136
Identity = 239/329 (72.64%), Postives = 280/329 (85.11%), Query Frame = 0


Query: 5   MSDELPVVKFCSENLKSGTKEWVFTMKKVREALEDYGCFVALYDSVSAEVSS-NMFDSLK 64
           M+ E+PVV+F S N++SG ++W  T +KVREALE+YGCFVAL DSVS E SS  M +++K
Sbjct: 1   MAGEIPVVRFSSHNVQSGGRKWGSTREKVREALEEYGCFVALCDSVSMEASSVKMMEAMK 60

Query: 65  ELFDDVPLERKLQNVSEKPYHGYFGQNPLMPIHESMGIEHPILPTNINSFTNLMWPSRGN 124
           E+F +VPLERK+QNVS+KP+HGYFGQNPLMPIHESMGIE P+LP+NI SF+NLMWPSR N
Sbjct: 61  EVF-EVPLERKVQNVSQKPFHGYFGQNPLMPIHESMGIEDPLLPSNIQSFSNLMWPSRPN 120

Query: 125 DSFRESVTTYAKLVSELDERVKRMVFESYGVGNALKSHMESTKYLMRMIKYRVPKEKEMN 184
             F  ++ +YA+LVS+L++ VK+MVFESYG+ +   SH +STKYL+RMIKYRVP   EMN
Sbjct: 121 PLFCGNLKSYAELVSQLEQTVKKMVFESYGLKSLFLSHTQSTKYLLRMIKYRVPHRNEMN 180

Query: 185 LGAFPHTDKSFLTILHQNEVNGLQIKTRDNKWIQYHPFSS-SSTSSFIVMAGDAFFAWSN 244
           LGAFPHTDKSFLTILHQN VNGL IKTRDN+W++Y P S+ SS  SFIVMAGDAFFAWSN
Sbjct: 181 LGAFPHTDKSFLTILHQNSVNGLHIKTRDNRWVEYQPPSAQSSARSFIVMAGDAFFAWSN 240

Query: 245 GRIYSPPHRVIMSGNRERYSVGLFSFNNGIVQIPKELVDDKHPQLFKPFDHQDYLRFYST 304
           GRIYS PHRV MSGNRERYSVGLFSFN+GIVQIPKELVDDKHPQ FK FDHQDYLRFYST
Sbjct: 241 GRIYSAPHRVTMSGNRERYSVGLFSFNDGIVQIPKELVDDKHPQQFKAFDHQDYLRFYST 300

Query: 305 ERGQNSPCAIKAYCGVGNSLVDMDTRSPL 332
           E+GQ+SP AIKAYCGV N L+D  T  P+
Sbjct: 301 EKGQSSPSAIKAYCGVANGLLDSRTCIPM 328

[Close]

BLAST of Cmc12g0336201 vs. TAIR 10
Match: AT1G52790.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 317.8 bits (813), Expect = 1.1e-86
Identity = 152/314 (48.41%), Postives = 216/314 (68.79%), Query Frame = 0


Query: 5   MSDELPVVKFCSENLKSGTKEWVFTMKKVREALEDYGCFVA-LYDSVSAEVSSNMFDSLK 64
           MS ++P + F  E+LK GTK W  T + +R+ALE+YGCF+  L D    ++   +F SL 
Sbjct: 1   MSPKIPTLDFSREDLKPGTKYWESTRENIRQALEEYGCFIIDLKDKTPLDLLDRVFGSLV 60

Query: 65  ELFDDVPLERKLQNVSEKPYHGYFGQNPLMPIHESMGIEHPILPTNINSFTNLMWPSRGN 124
           +LF D+P + K++N  +KP +GY GQ P +P+HES+GI++        SFT LMWP +GN
Sbjct: 61  DLF-DLPTQTKMKNKYDKPLNGYVGQIPALPLHESLGIDNATSLEATRSFTGLMWP-QGN 120

Query: 125 DSFRESVTTYAKLVSELDERVKRMVFESYGVGNALKSHMESTKYLMRMIKYRVPKEKEMN 184
           + F E +  YA+  +ELD+ V RMVF+SY V      ++EST YL+R++K R P  +   
Sbjct: 121 EHFSECLYKYAEFAAELDQMVTRMVFQSYNVEKYYDPYIESTTYLLRVLKNRAPNNENPT 180

Query: 185 LGAFPHTDKSFLTILHQNEVNGLQIKTRDNKWIQYHPFSSSSTSSFIVMAGDAFFAWSNG 244
           LG   HTDKSF TILHQ++VNGL+++TR+ + I     + SS S F+V+AGDA  AWSN 
Sbjct: 181 LGFVTHTDKSFTTILHQDQVNGLEMETREGERIN---INLSSPSLFMVVAGDALMAWSND 240

Query: 245 RIYSPPHRVIMSGNRERYSVGLFSFNNGIVQIPKELVDDKHPQLFKPFDHQDYLRFYSTE 304
           R++SP H+V++SG  +RYS+G+F+FNNG +Q+P+EL+D +HP ++KPFDH   LRFY T+
Sbjct: 241 RVWSPRHQVLVSGETDRYSLGMFAFNNGTLQVPEELIDHQHPLMYKPFDHLGLLRFYRTD 300

Query: 305 RGQNSPCAIKAYCG 318
            G  S C IKAYCG
Sbjct: 301 TGYKSECPIKAYCG 309

[Close]

BLAST of Cmc12g0336201 vs. TAIR 10
Match: AT1G52820.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 315.5 bits (807), Expect = 5.3e-86
Identity = 147/312 (47.12%), Postives = 219/312 (70.19%), Query Frame = 0


Query: 9   LPVVKFCSENLKSGTKEWVFTMKKVREALEDYGCFVALYDSVSAEVSSNMFDSLKELFDD 68
           LPV+ F ++NLK G  EW  T   V++AL+DYG F A +D +  E+  ++F +L+ELF D
Sbjct: 12  LPVIDFSNKNLKPGEPEWDLTRADVQKALQDYGYFEASFDRIPFELRKSVFGALEELF-D 71

Query: 69  VPLERKLQNVSEKPYHGYFGQNPLMPIHESMGIEHPILPTNINSFTNLMWPSRGNDSFRE 128
           +PL+ KL+NVS+KP+HGY GQ P++P++ESMGI+   +   +++FT  +WP +GN SF  
Sbjct: 72  LPLQTKLRNVSKKPFHGYVGQYPMVPLYESMGIDDSDIAEKVDAFTEKLWP-QGNISFST 131

Query: 129 SVTTYAKLVSELDERVKRMVFESYGVGNALKSHMESTKYLMRMIKYRVPKEKEMNLGAFP 188
           ++ +++K +SELD  ++RM+ ES+G+   +  H+ ST YL+R++KY+ P  +E  +G   
Sbjct: 132 TIQSFSKKLSELDITIRRMIMESFGLDKYIDEHLHSTNYLLRVMKYKGPDTEETKVGLNA 191

Query: 189 HTDKSFLTILHQNEVNGLQIKTRDNKWIQYHPFSSSSTSSFIVMAGDAFFAWSNGRIYSP 248
           HTDK+ +TIL+QN V GL+++T+D  WI+  P    +  SF VM GD+ +A  NGR++SP
Sbjct: 192 HTDKNIVTILYQNHVEGLEVQTKDKNWIKVKP----TQDSFTVMIGDSLYALLNGRLHSP 251

Query: 249 PHRVIMSGNRERYSVGLFSFNNG--IVQIPKELVDDKHPQLFKPFDHQDYLRFYSTERGQ 308
            HRV+M+G   RYS+GLFS      IV  P ELVD++HP+LFKPFDH ++L+FY TE GQ
Sbjct: 252 YHRVMMTGTETRYSLGLFSIPKAGHIVSSPDELVDEEHPRLFKPFDHVEFLQFYYTEAGQ 311

Query: 309 NSPCAIKAYCGV 319
            S  A+K YCG+
Sbjct: 312 RSQSALKTYCGI 317

[Close]

BLAST of Cmc12g0336201 vs. TAIR 10
Match: AT1G52800.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 302.4 bits (773), Expect = 4.6e-82
Identity = 145/313 (46.33%), Postives = 214/313 (68.37%), Query Frame = 0


Query: 8   ELPVVKFCS-ENLKSGTKEWVFTMKKVREALEDYGCFVALYDSVSAEVSSNMFDSLKELF 67
           ++P++   S + LK  T  W    ++  EALE+YGCF+A+YD V+ ++  ++F + +ELF
Sbjct: 9   KVPILDLTSQQELKPNTSTWRSVSREACEALEEYGCFLAVYDGVTQQLDDSIFAAAEELF 68

Query: 68  DDVPLERKLQNVSEKPYHGYFGQNPLMPIHESMGIEHPILPTNINSFTNLMWPSRGNDSF 127
            D+P E K +NV+EKPYHGY GQ P++P+HE +G+++         FT+LMWP +GND F
Sbjct: 69  -DLPTETKKKNVNEKPYHGYVGQMPVIPLHEGLGVDYVTNKEIAQRFTHLMWP-QGNDRF 128

Query: 128 RESVTTYAKLVSELDERVKRMVFESYGVGNALKSHMESTKYLMRMIKYRVPKEKEMNLGA 187
             +V T++  V+ELD  V RM+FE+YGV    +SH+ S  YL++ +KY  P E  +++ A
Sbjct: 129 CNTVHTFSNAVAELDRLVVRMIFENYGVEKHYESHVGSKTYLLKFLKYLAPPE-SISMPA 188

Query: 188 FP-HTDKSFLTILHQNEVNGLQIKTRDNKWIQYHPFSSSSTSSFIVMAGDAFFAWSNGRI 247
           FP HTDK+FL+ILHQN+VNGL++K++D +WI           S++VMAGD    WSN RI
Sbjct: 189 FPQHTDKTFLSILHQNDVNGLEVKSKDGEWISL----QLPPKSYVVMAGDISMGWSNDRI 248

Query: 248 YSPPHRVIMSGNRERYSVGLFSFNNGIVQIPKELVDDKHPQLFKPFDHQDYLRFYSTERG 307
            S  HRV M G++ RY++GLFSF   +V IP+ELVDDKHP ++KPFD+   + FY+T+ G
Sbjct: 249 RSCEHRVTMEGDKTRYTLGLFSFLTDLVSIPEELVDDKHPLMYKPFDNIALINFYTTKEG 308

Query: 308 QNSPCAIKAYCGV 319
           + +   +KAYCG+
Sbjct: 309 REANSTLKAYCGI 314

[Close]

BLAST of Cmc12g0336201 vs. TAIR 10
Match: AT4G03070.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 280.4 bits (716), Expect = 1.9e-75
Identity = 136/320 (42.50%), Postives = 210/320 (65.62%), Query Frame = 0


Query: 4   NMSDELPVVKFCSENLKSGTKEWVFTMKKVREALEDYGCFVALYDSVSAEVSSNMFDSLK 63
           ++S +LPV+ F  +NLK G+ +W      V +ALEDYGCF A +D +S E++ ++F++++
Sbjct: 9   SVSFQLPVIDFSDQNLKPGSSKWDEVTADVLKALEDYGCFEASFDKLSVELNRSVFEAME 68

Query: 64  ELFDDVPLERKLQNVSEKPYHGYFGQNPLMPIHESMGIEHPILPTNINSFTNLMWPSRGN 123
           +LF ++P+  K +NVS KP+HGY   N    ++ES+GI    +   +N FT  +WP  GN
Sbjct: 69  DLF-ELPIPTKQRNVSSKPFHGYLCHN----LYESLGINDANVLEKVNDFTQQLWPDHGN 128

Query: 124 DSFRESVTTYAKLVSELDERVKRMVFESYGVGNALKSHMESTKYLMRMIKYRVP------ 183
            S  E++  +++ + ELD  V+RM+ ES+G+ N +  H+ ST YL R++KY  P      
Sbjct: 129 KSISETIHLFSEQLVELDLMVRRMIMESFGIENYIDEHLNSTYYLTRLMKYTSPPDDDDD 188

Query: 184 KEKEMNLGAFPHTDKSFLTILHQNEVNGLQIKTRDNKWIQYHPFSSSSTSSFIVMAGDAF 243
            ++E  LG   HTDK+ +TILHQ +V+GL++KT+D+KWI+  P    S  S +VM GD+ 
Sbjct: 189 DDEETKLGLRSHTDKNIITILHQYQVDGLEVKTKDDKWIKVKP----SQDSVLVMVGDSL 248

Query: 244 FAWSNGRIYSPPHRVIMSGNRERYSVGLFSF--NNGIVQIPKELVDDKHPQLFKPFDHQD 303
            A  NGR++SP HRVIM+G + RYS GLFS      I+  P+ELVD +HP++FKPF++ D
Sbjct: 249 CALLNGRLHSPYHRVIMTGKKTRYSTGLFSIPKTGVIIDSPEELVDKEHPRIFKPFEYTD 308

Query: 304 YLRFYSTERGQNSPCAIKAY 316
           +L F+ TE G+ +  A+ A+
Sbjct: 309 FLHFFQTEAGRIAQSALHAF 319

[Close]

BLAST of Cmc12g0336201 vs. TAIR 10
Match: AT1G80320.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 252.7 bits (644), Expect = 4.2e-67
Identity = 130/316 (41.14%), Postives = 194/316 (61.39%), Query Frame = 0


Query: 9   LPVVKFCSENLKSGTKEWVFTMKKVREALEDYGCFVALYDSVSAEVSSNMFDSLKELFDD 68
           LP++ F S+ L  GT  W+ T   VR A+E  G FVA +  VS+++  N+   +KE++  
Sbjct: 9   LPILDFSSDKLVRGTSHWITTRDDVRRAMEGQGWFVAEFSGVSSDLRDNLLAGMKEMY-Y 68

Query: 69  VPLERKLQNVSEKPYHGYFGQ-NPLMPIHESMGIEHPILPTNINSFTNLMWPSRGNDSFR 128
           +P + K++N + K  HGY         IHES+GI++         F+ L+WP +GND F 
Sbjct: 69  LPDQIKIKNENHKASHGYMSMVVDDYRIHESLGIDYATELQACKDFSKLLWP-QGNDPFC 128

Query: 129 ESVTTYAKLVSELDERVKRMVFESYGVGNALK--SHMESTKYLMRMIKYRVPKEKEMNLG 188
           ++   YA  ++ELD+ V RM++ESYG+       SH EST+YL+RM+ YR  +  E N G
Sbjct: 129 QTTHMYAMTMAELDQTVMRMLYESYGMDEKKHSVSHSESTRYLLRMLSYRRQQNGEANTG 188

Query: 189 AFPHTDKSFLTILHQNEVNGLQIKTRDNKWIQYHPFSSSSTSSFIVMAGDAFFAWSNGRI 248
              HTDKSF++ILHQN V GLQ+KT   +W+ ++P    S + F+V++G    AWSN RI
Sbjct: 189 FVSHTDKSFMSILHQNHVGGLQLKTMTGQWVGFNP----SPTRFVVLSGMGLTAWSNDRI 248

Query: 249 YSPPHRVIMSGNRERYSVGLFSFNNGIVQIPKELVDDKHPQLFKPFDHQDYLRFYST--- 308
            +  H+V+MS +  RYS+G FSF+ G ++ P+ELVDD+HP  + PF+H   LRFY +   
Sbjct: 249 KACYHKVVMSADEIRYSLGFFSFHKGTIRTPEELVDDQHPLRYNPFEHDGLLRFYESYIN 308

Query: 309 ERGQNSPCAIKAYCGV 319
              ++S   ++ YCGV
Sbjct: 309 SLKKSSEDLLQIYCGV 318

The following BLAST results are available for this feature:

BLAST of Cmc12g0336201 vs. NCBI nr


Match Name	E-value	Identity	Description
TYJ99087.1	4.2e-194	98.80	putative 2-oxoglutarate-dependent dioxygenase AOP1 [Cucumis melo var. makuwa]	[more]
XP_031738134.1	9.3e-186	94.93	probable 2-oxoglutarate-dependent dioxygenase AOP1 [Cucumis sativus] >KAE8652571...	[more]
XP_004151475.3	1.2e-185	94.63	probable 2-oxoglutarate-dependent dioxygenase AOP1 [Cucumis sativus] >KAE8652570...	[more]
XP_038895877.1	9.4e-170	87.58	probable 2-oxoglutarate-dependent dioxygenase AOP1 [Benincasa hispida]	[more]
XP_038893580.1	6.1e-169	87.77	probable 2-oxoglutarate-dependent dioxygenase AOP1 [Benincasa hispida]	[more]

BLAST of Cmc12g0336201 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q945B6	6.9e-75	42.63	Probable 2-oxoglutarate-dependent dioxygenase AOP1.2 OS=Arabidopsis thaliana OX=...	[more]
Q9ZTA3	2.6e-74	42.50	Probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Arabidopsis thaliana OX=37...	[more]
Q944Z5	1.2e-71	42.72	Probable 2-oxoglutarate-dependent dioxygenase AOP1 (Fragment) OS=Arabidopsis tha...	[more]
A0A0N9HQ36	1.3e-60	40.07	Deoxypodophyllotoxin synthase OS=Sinopodophyllum hexandrum OX=93608 GN=2-ODD PE=...	[more]
Q945B5	4.7e-55	29.95	2-oxoglutarate-dependent dioxygenase AOP2 OS=Arabidopsis thaliana OX=3702 GN=AOP...	[more]

BLAST of Cmc12g0336201 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5D3BIZ7	2.0e-194	98.80	Putative 2-oxoglutarate-dependent dioxygenase AOP1 OS=Cucumis melo var. makuwa O...	[more]
A0A6J1C712	2.6e-149	79.88	probable 2-oxoglutarate-dependent dioxygenase AOP1 isoform X1 OS=Momordica chara...	[more]
A0A6J1EAT7	5.4e-139	73.86	probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Cucurbita moschata OX=3662...	[more]
A0A6J1E7C8	1.3e-137	73.25	probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Cucurbita moschata OX=3662...	[more]
A0A6J1KFF7	2.5e-136	72.64	probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Cucurbita maxima OX=3661 G...	[more]

BLAST of Cmc12g0336201 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G52790.1	1.1e-86	48.41	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT1G52820.1	5.3e-86	47.12	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT1G52800.1	4.6e-82	46.33	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT4G03070.1	1.9e-75	42.50	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT1G80320.1	4.2e-67	41.14	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Melon (Charmono) v1.1
Date Performed: 2022-10-13


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 334..334
None	No IPR available	PANTHER	PTHR47990:SF17	2-OXOGLUTARATE-DEPENDENT DIOXYGENASE AOP3-LIKE	coord: 6..317
None	No IPR available	PANTHER	PTHR47990	2-OXOGLUTARATE (2OG) AND FE(II)-DEPENDENT OXYGENASE SUPERFAMILY PROTEIN-RELATED	coord: 6..317
None	No IPR available	SUPERFAMILY	51197	Clavaminate synthase-like	coord: 7..305
IPR026992	Non-haem dioxygenase N-terminal domain	PFAM	PF14226	DIOX_N	coord: 25..88 e-value: 1.7E-7 score: 32.0
IPR027443	Isopenicillin N synthase-like superfamily	GENE3D	2.60.120.330		coord: 1..299 e-value: 4.1E-56 score: 192.9
IPR044861	Isopenicillin N synthase-like, Fe(2+) 2OG dioxygenase domain	PFAM	PF03171	2OG-FeII_Oxy	coord: 174..268 e-value: 5.3E-18 score: 65.4
IPR005123	Oxoglutarate/iron-dependent dioxygenase	PROSITE	PS51471	FE2OG_OXY	coord: 165..271 score: 10.976438

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cmc12g0336201.1	Cmc12g0336201.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0051213	dioxygenase activity
molecular_function	GO:0046872	metal ion binding