Cmc12g0335991 (gene) Melon (Charmono) v1.1

Overview
NameCmc12g0335991
Typegene
OrganismCucumis melo L. var. cantalupensis cv. Charmono (Melon (Charmono) v1.1)
DescriptionPectate lyase
LocationCMiso1.1chr12: 24719032 .. 24723788 (+)
RNA-Seq ExpressionCmc12g0335991
SyntenyCmc12g0335991
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TTTAGAAGATTATAAAAGAATGTACTACTTTTGAAATTAAAATCTTTAGACTATCCTTAATCATTTTCCCAAGTTCTTCCCTTTCCCACTCAGACCCTGACCCTTCATTTTCTAATTTCTTTCTTCTTCTCTACCTGCTTCTTCAATGGAGATGCCAATATTCACCAGAAGCTTTGCAATAGATGAAACAGAGGGTGTTGACTGTACATATTATTTGTCAGTGCGATCGTCTAGTTACTTTTTCCCCTTGTGGAAGAAGCTTCTCTTCTCAAGTCTCAACCCCAATGCATGCCTATTCTGCACTTTCTGAATGGGTGGACTTTGTGGTGAGAATCTGATCCTCTCATCTCATAAAAAAGTAATTTTCTTTTCTCACTGATGCTTCTTCTTCTGTCTTTTGGCATTTTTTTTTCTTTTTCCTTTATTTTTTTTGTCCATTACATCATTTCAATGCTTCTTATTATATGATAATGGGTGTGAAAAAGGATGTGTTTTTCTTCAACTTTCTCCATTCCATGACCAATATTACCCTACCCCCCCGCCCAAAAAGAAAAAAAAAAAAATGTTTAACTTTAGGGCTCTAATCCATATCTCTGGTTTTTATGTCTATCCCATTGAAAAAGAAAGTTGGATTCCATATTGTTTGGCCTAACTCTGAAATGAGTTGCGATTAACTACCTCAAAACTTTCACTCAGGGCTTGACCTCGAAACTTCCACTCGCTTATGCTTGAAATAAAGAAAAATACAAGTGGAAATTAATATTAGTCGAGGAGGATACAACAATGCAAAGCTTGAACACATAGCATTATCGTGTTAGGAAGGGTTCCATCCCAGATAATCACAGCCGTAACACACTATTCATTAAAATTGAGATTTTTTTCCATTCAACAGTTGTTTTTTTAATCAACGGTGGACCAACTAGTGATTAAAAATGAAAACCATGATAGGCTTCCTACGGTTTGGCGATAGGTGAGATGCCTTAACACGATGGTGCATTCCAAGTAGTGGAATCTTCCAAAACGTAAGCAAAATGTTAGTTTTCATTATTTTTCAATGATTGGTCATTTTGCATTAACATCATTCGATCGGGTCATCTATCCAAAAATTCCGTACATTACCACGACCCCCTATTTGCATTAATTGATTGTTTGTTAGAGCAATATGTCCAAAGTTTGAACTCTTAATTTATGTAATGTCATTTTTCTTTCTAATTTTCAAGCCCTTAAACGAAGAGGAAGATTGTTGAAAGGTAGGTATAATTAAGGTTTAGGAGTTCTGACGGGTGATTTAACATAAATCTGTACATATCTTCAATAATTATCTCATCCATTTCTTTCTTTTGTTGTGCATTACCCTAAGGAGAAATTTCTGGTGGTGTTTGTTTCTTTTGACTATGATTTATGTTATGTTCTTCAATAAGATAAGACTGATTCACCCATCATTTTGTGACTGCTTTTATTGGATACACAGTCTTATTTCAATAAGATTTTCACTTGTTTCACGCAAAGAATTTTCTTTCTTTATTAACTTAATCAAGATTCTTTTTTATTAGGTTATGAAAAGTAAATCCATTTGTTTCCCTTATCCCTAATCAGTTGAAGTAAGCTTAGTTTTAAATTCGTACTCGATCACAAGTTAGAGGTTTGATTCTTCCAAATTTTCATTTGTAATTACATTTTGCTTGGATGGCATTGCCATATTTTGGTTTGGTTTATACTTTACCACCCTTTTATCATAAATTTTATGAAAATTTTTCTTTTTAGAATAAAAAAAAAAAAAATTCTCCCTTTTGAAGGGAAAAGGTAGATTTATGCCATTATCTGGAAAATAACCCTCCAAATTAATATTGATTTTATTAATTTTTATGGTGTTTGAGACAAGCTGAGAAGGCTATATCTCTGCTTTATTTTATTCCTTTGCTTTATTTTATTTGTATCCAATAGCTTTTAAACAAAATTCTGTCCAATAGCAAAATTATGCTCTTTCTTGGCTCCAAAAAGGAATAATGTAGAGGACAATTGATTTTTCTGGTGATGATATCTTCAAAATCTGAAATTTTCTCTTAATCCAAATCTTGGGTTAATCTTTGTTTAGAATGGAACATACTTCAATTCTATTTTATTATTAGAAACATTCTTTACTTAATATGTAATTTTCTGTATTCTTGTTAGAACTAAATCTTGATTGCTGTTAGAGCTTCTGCATTCTAAATTTAGACGAAAATGAAGTACAAAACGTAATAATATTTTTGATTGAGGAAATTTCTCGTTATCTCTTTGGGGAGAATGTTAAAATCCTTACATGAGTTTATCTTTTCGTTGTTACATAGATTTAGTTTGAAAGGAATCATAGGATTTTTCGAGGGAGCGAGAAGCTCTCGATTTGGACTTAAGTAGCGAAAGATTTTTGTAATTACTACTTGGTTTTCATTCAGTTAGATCGGAGTCTCTTAGATTTTTTCTAACCGGCGGTTATTTATGTAACTATTAATCATTTGTCAATTTCTTATGAATGAAGAAGTAATTATTTCTTAATTTAGTTATAATTTCAAAGAACACAATTCCTTGAAAATGGATAAAGAAAGCAAATAAGGAGTTGACCATTGAAGGGGATATATGGTTCCCCTTTATTTCCTCTTCATTTCTCATTTACATATTTAATGAAGCTATAAATTTATCATTGTTAGATAATCATTCCAAATTCAAATGGATTATTGATAATTAACAGAGAAATTTACAAAGAAAAAGTTCCTCAAAGAGATTAGAGAAAATGAGAGAGACAAAAACCACCATTGTTGGGATTAAACTTCTAATGATATTTGGTTTTGTAATGATATTTCCTACACTAAAGGCTAATATTGCAGATTTTGATGAAGTCTGGCAGAAAAGAGCTATAGAAGCAAAAAAAGCTTCCCTTGAGGCCTACCAACCCCATCCAGAAGAAGTTACTAATAATTTCAACAAGCAAGTTCATAGGTAATTAACCCCATTTTCTCATTAGCAAATTAATGATTCTATGTTTTGTATGTATGTAATTCTGGCTTTTCTTAATTGTGAGACAGATCTTTAGATGGTGCAAATAACACAAGGAGACATTTGAGGAAGTACACTGGTCCATGTTTGGCCACGAATCCGATTGATCGGTGCTGGAGATGTGATAGAAATTGGGCTAGGAACCGCAAGAAACTAGCTGATTGTGCCCTTGGTTTTGGTCGTGGAACGACCGGAGGCAAAGATGGAAAGATTTACGTGGTCCGAGATTCGAGTGATAACGACTTGGTGAATCCTAAACCAGGAACCTTGCGCCATGCTGTGATTCAGGAGAGACCTCTTTGGATTATATTTGCTCATGACATGGTGATTAGGCTAAGTGAAGAGCTTATAATGACTGATAATAAAACATTAGATGGGCGCGGTGCCAATGTGCACATTGCTAATGGTGGTCAAATTACTCTACAATTTGTCAGGAATATAATCATTCATAATCTTCACATTCATGACATCAAGGCTGGCAATGGAGGCTTTATAAGAGATTCGGTGAGCCACTATGGATACCGAACACAGAGCGATGGTGATGGAATCTCAATGTTCGGATCCTCGAGGGTTTGGATAGATCATGTTTCAATGTCTAATTGTCAAGATGGACTAATAGATGCAGTCATGGCATCTACTGCCATTACCATTTCCAACTGTCACTTCACCCACCATAACGATGTAAGCCCCTCAAACCATAAAAGAAAGACAAAATAGCCTTAGTTTTATTTCTTTTTAAGGGATTTGAACATCTAACTTGTTGGTAGATGATGTTTATCTTCGGTGGGATGGCATTTCTTCTATATCGATGCTGATAACTGACTAAATTCTTTTGTGGATGAAGGTGATTTTATTAGGTGCAAGTGATAGCTACTCGAATGATAAGATAATGCAGGTAACTCTTGCATTTAATCACTTTGGTAAAGGGTTGGTGCAAAGGATGCCGAGATGCCGATGGGGATTTATTCATGTAGTCAATAATGACTACACTCACTGGCTTATGTACGCCATCGGCGGCAGCCACAATCCCACCATTATCAGCCAGGGCAACCGTTTCATTGCTCCGCCAAATCCAAATTCCAAAGAGGTATGAAGAACACAAGATTAGACAAATATCACTTATAATCACACTGCCACATCCATTCCTTAATAATTGGGTTCTTGAGTATTATCCATTGAAACCAACCTTGGGATCCTTTACAAAACACAAGAAACAATATCCTCCTATCTTACTTGTGTCCAAATGTATAGCAATGTATTGAGCAATTGTTATTGTCGGCCAATTTAGGTGACGAAAAGGGTGTATGCATCGGAACGCGAATGGAGATCTTGGAACTGGAGGTCAGAGGGAGATCTGATGATGAATGGAGCTTTTTTCATCCAATCTGGGAACCCCATTAGAAGATACTCCAAGAAGGATGTTATCCATTCGAAACCCGGGACCTTCGTCACCAGGCTTACACGATTTGCCGGCCCTTTGAAATGTAAAATGAATCAACCCTGTTAGTGAAATTCTTTACGTCACCTTCAAACTATGATATGTATATAAAATACTCAAAGTTCAAATGAACCCTGTAATCAGCAGAGGTTTTGAATAGTAAAGATTTAGATAGAAGATAAACGGACATAACTACGGCAGCTTTTGCCTTTGTAAAGTTGTGAATCACTTGTGTGATATTACCTATTGAAATAATATTGGTTATTCATCTTTTGAATGTTGTTTA

mRNA sequence

TTTAGAAGATTATAAAAGAATGTACTACTTTTGAAATTAAAATCTTTAGACTATCCTTAATCATTTTCCCAAGTTCTTCCCTTTCCCACTCAGACCCTGACCCTTCATTTTCTAATTTCTTTCTTCTTCTCTACCTGCTTCTTCAATGGAGATGCCAATATTCACCAGAAGCTTTGCAATAGATGAAACAGAGGGTGTTGACTGTACATATTATTTGTCAGTGCGATCGTCTAGTTACTTTTTCCCCTTGTGGAAGAAGCTTCTCTTCTCAAGTCTCAACCCCAATGCATGCCTATTCTGCACTTTCTGAATGGGTGGACTTTGTGATTTTGATGAAGTCTGGCAGAAAAGAGCTATAGAAGCAAAAAAAGCTTCCCTTGAGGCCTACCAACCCCATCCAGAAGAAGTTACTAATAATTTCAACAAGCAAGTTCATAGATCTTTAGATGGTGCAAATAACACAAGGAGACATTTGAGGAAGTACACTGGTCCATGTTTGGCCACGAATCCGATTGATCGGTGCTGGAGATGTGATAGAAATTGGGCTAGGAACCGCAAGAAACTAGCTGATTGTGCCCTTGGTTTTGGTCGTGGAACGACCGGAGGCAAAGATGGAAAGATTTACGTGGTCCGAGATTCGAGTGATAACGACTTGGTGAATCCTAAACCAGGAACCTTGCGCCATGCTGTGATTCAGGAGAGACCTCTTTGGATTATATTTGCTCATGACATGGTGATTAGGCTAAGTGAAGAGCTTATAATGACTGATAATAAAACATTAGATGGGCGCGGTGCCAATGTGCACATTGCTAATGGTGGTCAAATTACTCTACAATTTGTCAGGAATATAATCATTCATAATCTTCACATTCATGACATCAAGGCTGGCAATGGAGGCTTTATAAGAGATTCGGTGAGCCACTATGGATACCGAACACAGAGCGATGGTGATGGAATCTCAATGTTCGGATCCTCGAGGGTTTGGATAGATCATGTTTCAATGTCTAATTGTCAAGATGGACTAATAGATGCAGTCATGGCATCTACTGCCATTACCATTTCCAACTGTCACTTCACCCACCATAACGATGTGATTTTATTAGGTGCAAGTGATAGCTACTCGAATGATAAGATAATGCAGGTAACTCTTGCATTTAATCACTTTGGTAAAGGGTTGGTGCAAAGGATGCCGAGATGCCGATGGGGATTTATTCATGTAGTCAATAATGACTACACTCACTGGCTTATGTACGCCATCGGCGGCAGCCACAATCCCACCATTATCAGCCAGGGCAACCGTTTCATTGCTCCGCCAAATCCAAATTCCAAAGAGGTGACGAAAAGGGTGTATGCATCGGAACGCGAATGGAGATCTTGGAACTGGAGGTCAGAGGGAGATCTGATGATGAATGGAGCTTTTTTCATCCAATCTGGGAACCCCATTAGAAGATACTCCAAGAAGGATGTTATCCATTCGAAACCCGGGACCTTCGTCACCAGGCTTACACGATTTGCCGGCCCTTTGAAATGTAAAATGAATCAACCCTGTTAGTGAAATTCTTTACGTCACCTTCAAACTATGATATGTATATAAAATACTCAAAGTTCAAATGAACCCTGTAATCAGCAGAGGTTTTGAATAGTAAAGATTTAGATAGAAGATAAACGGACATAACTACGGCAGCTTTTGCCTTTGTAAAGTTGTGAATCACTTGTGTGATATTACCTATTGAAATAATATTGGTTATTCATCTTTTGAATGTTGTTTA

Coding sequence (CDS)

ATGGGTGGACTTTGTGATTTTGATGAAGTCTGGCAGAAAAGAGCTATAGAAGCAAAAAAAGCTTCCCTTGAGGCCTACCAACCCCATCCAGAAGAAGTTACTAATAATTTCAACAAGCAAGTTCATAGATCTTTAGATGGTGCAAATAACACAAGGAGACATTTGAGGAAGTACACTGGTCCATGTTTGGCCACGAATCCGATTGATCGGTGCTGGAGATGTGATAGAAATTGGGCTAGGAACCGCAAGAAACTAGCTGATTGTGCCCTTGGTTTTGGTCGTGGAACGACCGGAGGCAAAGATGGAAAGATTTACGTGGTCCGAGATTCGAGTGATAACGACTTGGTGAATCCTAAACCAGGAACCTTGCGCCATGCTGTGATTCAGGAGAGACCTCTTTGGATTATATTTGCTCATGACATGGTGATTAGGCTAAGTGAAGAGCTTATAATGACTGATAATAAAACATTAGATGGGCGCGGTGCCAATGTGCACATTGCTAATGGTGGTCAAATTACTCTACAATTTGTCAGGAATATAATCATTCATAATCTTCACATTCATGACATCAAGGCTGGCAATGGAGGCTTTATAAGAGATTCGGTGAGCCACTATGGATACCGAACACAGAGCGATGGTGATGGAATCTCAATGTTCGGATCCTCGAGGGTTTGGATAGATCATGTTTCAATGTCTAATTGTCAAGATGGACTAATAGATGCAGTCATGGCATCTACTGCCATTACCATTTCCAACTGTCACTTCACCCACCATAACGATGTGATTTTATTAGGTGCAAGTGATAGCTACTCGAATGATAAGATAATGCAGGTAACTCTTGCATTTAATCACTTTGGTAAAGGGTTGGTGCAAAGGATGCCGAGATGCCGATGGGGATTTATTCATGTAGTCAATAATGACTACACTCACTGGCTTATGTACGCCATCGGCGGCAGCCACAATCCCACCATTATCAGCCAGGGCAACCGTTTCATTGCTCCGCCAAATCCAAATTCCAAAGAGGTGACGAAAAGGGTGTATGCATCGGAACGCGAATGGAGATCTTGGAACTGGAGGTCAGAGGGAGATCTGATGATGAATGGAGCTTTTTTCATCCAATCTGGGAACCCCATTAGAAGATACTCCAAGAAGGATGTTATCCATTCGAAACCCGGGACCTTCGTCACCAGGCTTACACGATTTGCCGGCCCTTTGAAATGTAAAATGAATCAACCCTGTTAG

Protein sequence

MGGLCDFDEVWQKRAIEAKKASLEAYQPHPEEVTNNFNKQVHRSLDGANNTRRHLRKYTGPCLATNPIDRCWRCDRNWARNRKKLADCALGFGRGTTGGKDGKIYVVRDSSDNDLVNPKPGTLRHAVIQERPLWIIFAHDMVIRLSEELIMTDNKTLDGRGANVHIANGGQITLQFVRNIIIHNLHIHDIKAGNGGFIRDSVSHYGYRTQSDGDGISMFGSSRVWIDHVSMSNCQDGLIDAVMASTAITISNCHFTHHNDVILLGASDSYSNDKIMQVTLAFNHFGKGLVQRMPRCRWGFIHVVNNDYTHWLMYAIGGSHNPTIISQGNRFIAPPNPNSKEVTKRVYASEREWRSWNWRSEGDLMMNGAFFIQSGNPIRRYSKKDVIHSKPGTFVTRLTRFAGPLKCKMNQPC
Homology
BLAST of Cmc12g0335991 vs. NCBI nr
Match: TYJ99102.1 (pectate lyase-like isoform X2 [Cucumis melo var. makuwa])

HSP 1 Score: 865.5 bits (2235), Expect = 1.8e-247
Identity = 408/410 (99.51%), Postives = 409/410 (99.76%), Query Frame = 0

Query: 4   LCDFDEVWQKRAIEAKKASLEAYQPHPEEVTNNFNKQVHRSLDGANNTRRHLRKYTGPCL 63
           + DFDEVWQKRAIEAKKASLEAYQPHPEEVTNNFNKQVHRSLDGANNTRRHLRKYTGPCL
Sbjct: 30  IADFDEVWQKRAIEAKKASLEAYQPHPEEVTNNFNKQVHRSLDGANNTRRHLRKYTGPCL 89

Query: 64  ATNPIDRCWRCDRNWARNRKKLADCALGFGRGTTGGKDGKIYVVRDSSDNDLVNPKPGTL 123
           ATNPIDRCWRCDRNWARNRKKLADCALGFGRGTTGGKDGKIYVVRDSSDNDLVNPKPGTL
Sbjct: 90  ATNPIDRCWRCDRNWARNRKKLADCALGFGRGTTGGKDGKIYVVRDSSDNDLVNPKPGTL 149

Query: 124 RHAVIQERPLWIIFAHDMVIRLSEELIMTDNKTLDGRGANVHIANGGQITLQFVRNIIIH 183
           RHAVIQERPLWIIFAHDMVIRLSEELIMTDNKTLDGRGANVHIANGGQITLQFVRNIIIH
Sbjct: 150 RHAVIQERPLWIIFAHDMVIRLSEELIMTDNKTLDGRGANVHIANGGQITLQFVRNIIIH 209

Query: 184 NLHIHDIKAGNGGFIRDSVSHYGYRTQSDGDGISMFGSSRVWIDHVSMSNCQDGLIDAVM 243
           NLHIHDIKAGNGGFIRDSVSHYGYRTQSDGDGISMFGSSRVWIDHVSMSNCQDGLIDAVM
Sbjct: 210 NLHIHDIKAGNGGFIRDSVSHYGYRTQSDGDGISMFGSSRVWIDHVSMSNCQDGLIDAVM 269

Query: 244 ASTAITISNCHFTHHNDVILLGASDSYSNDKIMQVTLAFNHFGKGLVQRMPRCRWGFIHV 303
           ASTAITISNCHFTHHNDVILLGASDSYSNDKIMQVTLAFNHFGKGLVQRMPRCRWGFIHV
Sbjct: 270 ASTAITISNCHFTHHNDVILLGASDSYSNDKIMQVTLAFNHFGKGLVQRMPRCRWGFIHV 329

Query: 304 VNNDYTHWLMYAIGGSHNPTIISQGNRFIAPPNPNSKEVTKRVYASEREWRSWNWRSEGD 363
           VNNDYTHWLMYAIGGSHNPTIISQGNRFIAPPNPNSKEVTKRVYASEREWRSWNWRSEGD
Sbjct: 330 VNNDYTHWLMYAIGGSHNPTIISQGNRFIAPPNPNSKEVTKRVYASEREWRSWNWRSEGD 389

Query: 364 LMMNGAFFIQSGNPIRRYSKKDVIHSKPGTFVTRLTRFAGPLKCKMNQPC 414
           LMMNGAFFIQSGNPIRRYSKKDVIHSKPGTFVTRLTRFAGPLKCKMNQPC
Sbjct: 390 LMMNGAFFIQSGNPIRRYSKKDVIHSKPGTFVTRLTRFAGPLKCKMNQPC 439

BLAST of Cmc12g0335991 vs. NCBI nr
Match: XP_011651190.1 (pectate lyase isoform X2 [Cucumis sativus])

HSP 1 Score: 839.3 bits (2167), Expect = 1.4e-239
Identity = 392/413 (94.92%), Postives = 403/413 (97.58%), Query Frame = 0

Query: 1   MGGLCDFDEVWQKRAIEAKKASLEAYQPHPEEVTNNFNKQVHRSLDGANNTRRHLRKYTG 60
           MGGLCDFDEVWQKRAIEA+KAS EAY+PHPEE TNNFNKQVHRSLDG NNTRRHLRKYTG
Sbjct: 1   MGGLCDFDEVWQKRAIEAQKASFEAYEPHPEEETNNFNKQVHRSLDGGNNTRRHLRKYTG 60

Query: 61  PCLATNPIDRCWRCDRNWARNRKKLADCALGFGRGTTGGKDGKIYVVRDSSDNDLVNPKP 120
           PCLATNPIDRCWRCDRNWARNRKKLADCALGFGR TTGGKDGKIYVVRDSSDNDLVNPKP
Sbjct: 61  PCLATNPIDRCWRCDRNWARNRKKLADCALGFGRRTTGGKDGKIYVVRDSSDNDLVNPKP 120

Query: 121 GTLRHAVIQERPLWIIFAHDMVIRLSEELIMTDNKTLDGRGANVHIANGGQITLQFVRNI 180
           GTLRHAVIQERPLWIIFAHDMVIRLSEELI+TD+KTLDGRGANVHIANGGQITLQFV+NI
Sbjct: 121 GTLRHAVIQERPLWIIFAHDMVIRLSEELIVTDDKTLDGRGANVHIANGGQITLQFVKNI 180

Query: 181 IIHNLHIHDIKAGNGGFIRDSVSHYGYRTQSDGDGISMFGSSRVWIDHVSMSNCQDGLID 240
           IIHNLHIHDIKAGNGG IRDSVSHYG+RT+SDGDGISMFG+SRVWIDHVSMSNCQDGLID
Sbjct: 181 IIHNLHIHDIKAGNGGMIRDSVSHYGFRTRSDGDGISMFGASRVWIDHVSMSNCQDGLID 240

Query: 241 AVMASTAITISNCHFTHHNDVILLGASDSYSNDKIMQVTLAFNHFGKGLVQRMPRCRWGF 300
           AVMASTAITISNCHFTHHNDVILLGAS+ YSND+IMQVTLAFNHFGKGLVQRMPRCRWGF
Sbjct: 241 AVMASTAITISNCHFTHHNDVILLGASNGYSNDQIMQVTLAFNHFGKGLVQRMPRCRWGF 300

Query: 301 IHVVNNDYTHWLMYAIGGSHNPTIISQGNRFIAPPNPNSKEVTKRVYASEREWRSWNWRS 360
           IHVVNNDYTHWLMYAIGGSHNPTIISQGNRFIAPPNPN KEVTKRVYA E EWRSWNWRS
Sbjct: 301 IHVVNNDYTHWLMYAIGGSHNPTIISQGNRFIAPPNPNCKEVTKRVYAPESEWRSWNWRS 360

Query: 361 EGDLMMNGAFFIQSGNPIRRYSKKDVIHSKPGTFVTRLTRFAGPLKCKMNQPC 414
           EGDLMMNGAFFIQSGNPI+RYSKKDVIHSKPGTFVTRLTRFAGPLKCK NQPC
Sbjct: 361 EGDLMMNGAFFIQSGNPIKRYSKKDVIHSKPGTFVTRLTRFAGPLKCKKNQPC 413

BLAST of Cmc12g0335991 vs. NCBI nr
Match: XP_004152537.2 (pectate lyase isoform X1 [Cucumis sativus] >KGN64253.1 hypothetical protein Csa_013613 [Cucumis sativus])

HSP 1 Score: 828.6 bits (2139), Expect = 2.4e-236
Identity = 387/410 (94.39%), Postives = 399/410 (97.32%), Query Frame = 0

Query: 4   LCDFDEVWQKRAIEAKKASLEAYQPHPEEVTNNFNKQVHRSLDGANNTRRHLRKYTGPCL 63
           + DFDEVWQKRAIEA+KAS EAY+PHPEE TNNFNKQVHRSLDG NNTRRHLRKYTGPCL
Sbjct: 30  IADFDEVWQKRAIEAQKASFEAYEPHPEEETNNFNKQVHRSLDGGNNTRRHLRKYTGPCL 89

Query: 64  ATNPIDRCWRCDRNWARNRKKLADCALGFGRGTTGGKDGKIYVVRDSSDNDLVNPKPGTL 123
           ATNPIDRCWRCDRNWARNRKKLADCALGFGR TTGGKDGKIYVVRDSSDNDLVNPKPGTL
Sbjct: 90  ATNPIDRCWRCDRNWARNRKKLADCALGFGRRTTGGKDGKIYVVRDSSDNDLVNPKPGTL 149

Query: 124 RHAVIQERPLWIIFAHDMVIRLSEELIMTDNKTLDGRGANVHIANGGQITLQFVRNIIIH 183
           RHAVIQERPLWIIFAHDMVIRLSEELI+TD+KTLDGRGANVHIANGGQITLQFV+NIIIH
Sbjct: 150 RHAVIQERPLWIIFAHDMVIRLSEELIVTDDKTLDGRGANVHIANGGQITLQFVKNIIIH 209

Query: 184 NLHIHDIKAGNGGFIRDSVSHYGYRTQSDGDGISMFGSSRVWIDHVSMSNCQDGLIDAVM 243
           NLHIHDIKAGNGG IRDSVSHYG+RT+SDGDGISMFG+SRVWIDHVSMSNCQDGLIDAVM
Sbjct: 210 NLHIHDIKAGNGGMIRDSVSHYGFRTRSDGDGISMFGASRVWIDHVSMSNCQDGLIDAVM 269

Query: 244 ASTAITISNCHFTHHNDVILLGASDSYSNDKIMQVTLAFNHFGKGLVQRMPRCRWGFIHV 303
           ASTAITISNCHFTHHNDVILLGAS+ YSND+IMQVTLAFNHFGKGLVQRMPRCRWGFIHV
Sbjct: 270 ASTAITISNCHFTHHNDVILLGASNGYSNDQIMQVTLAFNHFGKGLVQRMPRCRWGFIHV 329

Query: 304 VNNDYTHWLMYAIGGSHNPTIISQGNRFIAPPNPNSKEVTKRVYASEREWRSWNWRSEGD 363
           VNNDYTHWLMYAIGGSHNPTIISQGNRFIAPPNPN KEVTKRVYA E EWRSWNWRSEGD
Sbjct: 330 VNNDYTHWLMYAIGGSHNPTIISQGNRFIAPPNPNCKEVTKRVYAPESEWRSWNWRSEGD 389

Query: 364 LMMNGAFFIQSGNPIRRYSKKDVIHSKPGTFVTRLTRFAGPLKCKMNQPC 414
           LMMNGAFFIQSGNPI+RYSKKDVIHSKPGTFVTRLTRFAGPLKCK NQPC
Sbjct: 390 LMMNGAFFIQSGNPIKRYSKKDVIHSKPGTFVTRLTRFAGPLKCKKNQPC 439

BLAST of Cmc12g0335991 vs. NCBI nr
Match: XP_031735989.1 (pectate lyase isoform X3 [Cucumis sativus])

HSP 1 Score: 824.7 bits (2129), Expect = 3.5e-235
Identity = 388/413 (93.95%), Postives = 399/413 (96.61%), Query Frame = 0

Query: 1   MGGLCDFDEVWQKRAIEAKKASLEAYQPHPEEVTNNFNKQVHRSLDGANNTRRHLRKYTG 60
           MGGLC    VWQKRAIEA+KAS EAY+PHPEE TNNFNKQVHRSLDG NNTRRHLRKYTG
Sbjct: 1   MGGLC----VWQKRAIEAQKASFEAYEPHPEEETNNFNKQVHRSLDGGNNTRRHLRKYTG 60

Query: 61  PCLATNPIDRCWRCDRNWARNRKKLADCALGFGRGTTGGKDGKIYVVRDSSDNDLVNPKP 120
           PCLATNPIDRCWRCDRNWARNRKKLADCALGFGR TTGGKDGKIYVVRDSSDNDLVNPKP
Sbjct: 61  PCLATNPIDRCWRCDRNWARNRKKLADCALGFGRRTTGGKDGKIYVVRDSSDNDLVNPKP 120

Query: 121 GTLRHAVIQERPLWIIFAHDMVIRLSEELIMTDNKTLDGRGANVHIANGGQITLQFVRNI 180
           GTLRHAVIQERPLWIIFAHDMVIRLSEELI+TD+KTLDGRGANVHIANGGQITLQFV+NI
Sbjct: 121 GTLRHAVIQERPLWIIFAHDMVIRLSEELIVTDDKTLDGRGANVHIANGGQITLQFVKNI 180

Query: 181 IIHNLHIHDIKAGNGGFIRDSVSHYGYRTQSDGDGISMFGSSRVWIDHVSMSNCQDGLID 240
           IIHNLHIHDIKAGNGG IRDSVSHYG+RT+SDGDGISMFG+SRVWIDHVSMSNCQDGLID
Sbjct: 181 IIHNLHIHDIKAGNGGMIRDSVSHYGFRTRSDGDGISMFGASRVWIDHVSMSNCQDGLID 240

Query: 241 AVMASTAITISNCHFTHHNDVILLGASDSYSNDKIMQVTLAFNHFGKGLVQRMPRCRWGF 300
           AVMASTAITISNCHFTHHNDVILLGAS+ YSND+IMQVTLAFNHFGKGLVQRMPRCRWGF
Sbjct: 241 AVMASTAITISNCHFTHHNDVILLGASNGYSNDQIMQVTLAFNHFGKGLVQRMPRCRWGF 300

Query: 301 IHVVNNDYTHWLMYAIGGSHNPTIISQGNRFIAPPNPNSKEVTKRVYASEREWRSWNWRS 360
           IHVVNNDYTHWLMYAIGGSHNPTIISQGNRFIAPPNPN KEVTKRVYA E EWRSWNWRS
Sbjct: 301 IHVVNNDYTHWLMYAIGGSHNPTIISQGNRFIAPPNPNCKEVTKRVYAPESEWRSWNWRS 360

Query: 361 EGDLMMNGAFFIQSGNPIRRYSKKDVIHSKPGTFVTRLTRFAGPLKCKMNQPC 414
           EGDLMMNGAFFIQSGNPI+RYSKKDVIHSKPGTFVTRLTRFAGPLKCK NQPC
Sbjct: 361 EGDLMMNGAFFIQSGNPIKRYSKKDVIHSKPGTFVTRLTRFAGPLKCKKNQPC 409

BLAST of Cmc12g0335991 vs. NCBI nr
Match: XP_038894610.1 (pectate lyase-like isoform X2 [Benincasa hispida])

HSP 1 Score: 814.7 bits (2103), Expect = 3.7e-232
Identity = 378/410 (92.20%), Postives = 397/410 (96.83%), Query Frame = 0

Query: 4   LCDFDEVWQKRAIEAKKASLEAYQPHPEEVTNNFNKQVHRSLDGANNTRRHLRKYTGPCL 63
           + DFDEVWQKRA EAKKAS EAYQPHPEEVT+NFNKQVH+SLDGANNTRRHLRKY GPCL
Sbjct: 30  IADFDEVWQKRAEEAKKASFEAYQPHPEEVTSNFNKQVHKSLDGANNTRRHLRKYNGPCL 89

Query: 64  ATNPIDRCWRCDRNWARNRKKLADCALGFGRGTTGGKDGKIYVVRDSSDNDLVNPKPGTL 123
           ATNPIDRCWRCD++WA+NRKKLADCALGFGRGTTGGK+G+IYVVRDSSDNDL+NPKPGTL
Sbjct: 90  ATNPIDRCWRCDKHWAKNRKKLADCALGFGRGTTGGKNGRIYVVRDSSDNDLLNPKPGTL 149

Query: 124 RHAVIQERPLWIIFAHDMVIRLSEELIMTDNKTLDGRGANVHIANGGQITLQFVRNIIIH 183
           R+ VIQ+RPLWIIFAHDMVIRLSEELIMTDNKTLDGRGANVHIANGGQ+TLQFV+NIIIH
Sbjct: 150 RYGVIQDRPLWIIFAHDMVIRLSEELIMTDNKTLDGRGANVHIANGGQLTLQFVKNIIIH 209

Query: 184 NLHIHDIKAGNGGFIRDSVSHYGYRTQSDGDGISMFGSSRVWIDHVSMSNCQDGLIDAVM 243
           NLHIHDIKAGNGG IRDSV+HYG+RT SDGDGISMFG+S+VWIDHVSMSNCQDGLIDAVM
Sbjct: 210 NLHIHDIKAGNGGIIRDSVNHYGFRTPSDGDGISMFGASKVWIDHVSMSNCQDGLIDAVM 269

Query: 244 ASTAITISNCHFTHHNDVILLGASDSYSNDKIMQVTLAFNHFGKGLVQRMPRCRWGFIHV 303
           AST ITISNCHFT HNDVILLGASDSYSND+IMQVTLAFNHFGKGLVQRMPRCRWGFIHV
Sbjct: 270 ASTGITISNCHFTRHNDVILLGASDSYSNDQIMQVTLAFNHFGKGLVQRMPRCRWGFIHV 329

Query: 304 VNNDYTHWLMYAIGGSHNPTIISQGNRFIAPPNPNSKEVTKRVYASEREWRSWNWRSEGD 363
           VNNDYTHWLMYAIGGSHNPTIISQGNRFIAPPNPNSKEVTKR YASE EWRSWNWRSEGD
Sbjct: 330 VNNDYTHWLMYAIGGSHNPTIISQGNRFIAPPNPNSKEVTKRAYASESEWRSWNWRSEGD 389

Query: 364 LMMNGAFFIQSGNPIRRYSKKDVIHSKPGTFVTRLTRFAGPLKCKMNQPC 414
           LM+NGAFFIQSGNPIRRYSKKDVIHSKPGTFVTRLTRFAGPLKC  NQPC
Sbjct: 390 LMLNGAFFIQSGNPIRRYSKKDVIHSKPGTFVTRLTRFAGPLKCIKNQPC 439

BLAST of Cmc12g0335991 vs. ExPASy Swiss-Prot
Match: P40973 (Pectate lyase OS=Lilium longiflorum OX=4690 PE=2 SV=1)

HSP 1 Score: 585.5 bits (1508), Expect = 4.7e-166
Identity = 271/412 (65.78%), Postives = 328/412 (79.61%), Query Frame = 0

Query: 4   LCDFDEVWQKRAIEAKKASLEAYQPHPEEVTNNFNKQVHRSLDGANNTRRHLR-KYTGPC 63
           + +FDE WQK++  A+  + +AY PHPEEVTN+FNK VH S +G N+TRR+LR    G C
Sbjct: 24  IAEFDEYWQKKSKVAQAKAKKAYTPHPEEVTNHFNKAVHSSFEG-NSTRRNLRTNKLGQC 83

Query: 64  LATNPIDRCWRCDRNWARNRKKLADCALGFGRGTTGGKDGKIYVVRDSSDNDLVNPKPGT 123
           LATNPIDRCWRC +NW+ NRK L  C  GFGR TTGG  G+IYVV D SD+ L +PK GT
Sbjct: 84  LATNPIDRCWRCKKNWSANRKDLVKCVKGFGRKTTGGAAGEIYVVTDPSDDSLTDPKFGT 143

Query: 124 LRHAVIQERPLWIIFAHDMVIRLSEELIMTDNKTLDGRGANVHIANGGQITLQFVRNIII 183
           LR  VIQ+RPLWIIF   MVIRL +ELI+ ++KT+DGRGANV IA G Q+T+QFV N+II
Sbjct: 144 LRWGVIQDRPLWIIFGKSMVIRLKQELIINNDKTIDGRGANVQIAGGAQLTVQFVHNVII 203

Query: 184 HNLHIHDIKAGNGGFIRDSVSHYGYRTQSDGDGISMFGSSRVWIDHVSMSNCQDGLIDAV 243
           H +HIHDIK G GG IRDS  H G RT+SDGDGIS+ GSS +WIDHVS++ C DGLID +
Sbjct: 204 HGIHIHDIKPGEGGLIRDSEKHSGIRTRSDGDGISIIGSSNIWIDHVSLARCSDGLIDVI 263

Query: 244 MASTAITISNCHFTHHNDVILLGASDSYSNDKIMQVTLAFNHFGKGLVQRMPRCRWGFIH 303
           + STAITISNCH T H+DV+LLGASD+Y+ D+IMQVT+AFNHFG+GLVQRMPRCR+GF+H
Sbjct: 264 LGSTAITISNCHLTEHDDVMLLGASDTYTQDEIMQVTVAFNHFGRGLVQRMPRCRYGFVH 323

Query: 304 VVNNDYTHWLMYAIGGSHNPTIISQGNRFIAPPNPNSKEVTKRVYASEREWRSWNWRSEG 363
           VVNNDYTHW+MYA+GGS +PTIISQGNR+IAP    +KEVTKR YA   EW  W W+S+G
Sbjct: 324 VVNNDYTHWIMYAVGGSQHPTIISQGNRYIAPHIEAAKEVTKRDYAEPAEWSKWTWKSQG 383

Query: 364 DLMMNGAFFIQSGNPI-RRYSKKDVIHSKPGTFVTRLTRFAGPLKCKMNQPC 414
           DL ++GAFF++SG P   +YSKKD+I +KPGTFV RLTRF+G L CK N  C
Sbjct: 384 DLFVSGAFFVESGGPFENKYSKKDLIKAKPGTFVQRLTRFSGALNCKENMEC 434

BLAST of Cmc12g0335991 vs. ExPASy Swiss-Prot
Match: P15722 (Probable pectate lyase P59 OS=Solanum lycopersicum OX=4081 GN=LAT59 PE=2 SV=1)

HSP 1 Score: 549.3 bits (1414), Expect = 3.8e-155
Identity = 265/426 (62.21%), Postives = 318/426 (74.65%), Query Frame = 0

Query: 1   MGGLCDFDEVWQKRAIEAKKASLEAYQPHPEEVTNNFNKQVH------RSLDGA--NNTR 60
           M  +  +DEVW++RA EAK+ +   Y+PHPE VT  FN+++       + + G   N+TR
Sbjct: 25  MAHIGHYDEVWRRRAEEAKEYARNIYEPHPENVTLAFNQKLRDTMKELKKVKGTHNNSTR 84

Query: 61  RHL--RKYTGPCLATNPIDRCWRCDRNWARNRKKLADCALGFGRGTTGGKDGKIYVVRDS 120
           R L  +KYTGPC+ TNPID+CWRCD NWA NRKKLADCA+GFG    GGKDG+ YVV D+
Sbjct: 85  RGLGTKKYTGPCMVTNPIDKCWRCDPNWADNRKKLADCAMGFGSKAIGGKDGEFYVVTDN 144

Query: 121 SDNDLVNPKPGTLRHAVIQERPLWIIFAHDMVIRLSEELIMTDNKTLDGRGANVHIANGG 180
           SD D  +PKPGTLRHAVIQ+ PLWIIF   M IRL +E+IM  +KT+D RG NVHI  G 
Sbjct: 145 SD-DYNDPKPGTLRHAVIQKEPLWIIFKRGMNIRLHQEMIMQSDKTIDARGVNVHITKGA 204

Query: 181 QITLQFVRNIIIHNLHIHDIKAGNGGFIRDSVSHYGYRTQSDGDGISMFGSSRVWIDHVS 240
            ITLQ+++N+IIH LHIHDI  GNGG +RD+V H G RT+SDGDGIS+FG+S +WIDHVS
Sbjct: 205 GITLQYIKNVIIHGLHIHDIVEGNGGMVRDAVDHIGIRTKSDGDGISIFGASYIWIDHVS 264

Query: 241 MSNCQDGLIDAVMASTAITISNCHFTHHNDVILLGASDSYSNDKIMQVTLAFNHFGKGLV 300
           M  C DGLIDAV  ST ITISN HFT HN+V+L GASDS S D++MQ+TLAFNHFGK L+
Sbjct: 265 MQRCYDGLIDAVEGSTGITISNGHFTDHNEVMLFGASDSSSIDQVMQITLAFNHFGKRLI 324

Query: 301 QRMPRCRWGFIHVVNNDYTHWLMYAIGGSHNPTIISQGNRFIAPPNPNSKEVTKRVYASE 360
           QRMPRCRWG+IHVVNNDYTHW MYAIGGS +PTII QGNRFIAPP+   K+VTKR Y  E
Sbjct: 325 QRMPRCRWGYIHVVNNDYTHWNMYAIGGSMHPTIIHQGNRFIAPPDIFKKQVTKREYNPE 384

Query: 361 REWRSWNWRSEGDLMMNGAFFIQSGNPIRRYSKKDV---IHSKPGTFVTRLTRFAGPLKC 414
             W  W WRSEG+L MNGA+F +SG+P      KD+   I + P   VT +TRFAG L C
Sbjct: 385 SVWMQWTWRSEGNLFMNGAYFTESGDPEWSSKHKDLYDGISAAPAEDVTWMTRFAGVLGC 444

BLAST of Cmc12g0335991 vs. ExPASy Swiss-Prot
Match: Q9M9S2 (Probable pectate lyase 3 OS=Arabidopsis thaliana OX=3702 GN=AT59 PE=2 SV=1)

HSP 1 Score: 547.4 bits (1409), Expect = 1.4e-154
Identity = 258/431 (59.86%), Postives = 317/431 (73.55%), Query Frame = 0

Query: 2   GGLCDFDEVWQKRAIEAKKASLEAYQPHPEEVTNNFNKQVHRSLDGA---NNTRRHL--- 61
           G +  FD  W +R  +A K ++ +Y PHP  VTN+FN  V+ ++D +   N+TRR L   
Sbjct: 29  GNVAVFDSYWTQRQSDALKQTIGSYDPHPLNVTNHFNYHVNIAVDASESRNDTRRELTQV 88

Query: 62  ------RKYTGPCLATNPIDRCWRCDRNWARNRKKLADCALGFGRGTTGGKDGKIYVVRD 121
                  K +G CLA NPID CWRCDRNWA NRKKLADC LGFGR TTGGKDG IYVV+D
Sbjct: 89  RSGRKTHKSSGKCLAYNPIDNCWRCDRNWANNRKKLADCVLGFGRRTTGGKDGPIYVVKD 148

Query: 122 SSDNDLVNPKPGTLRHAVIQERPLWIIFAHDMVIRLSEELIMTDNKTLDGRGANVHIANG 181
           +SDNDL+NPKPGTLRHAV ++ PLWIIFA  M+I+L +EL++T +KT+DGRGA V+I  G
Sbjct: 149 ASDNDLINPKPGTLRHAVTRDGPLWIIFARSMIIKLQQELMITSDKTIDGRGARVYIMEG 208

Query: 182 GQITLQFVRNIIIHNLHIHDIKAGNGGFIRDSVSHYGYRTQSDGDGISMFGSSRVWIDHV 241
             +TLQFV N+IIHN+++  I  GNGG IRDS +H G RT+SDGDGIS+FG++ +WIDHV
Sbjct: 209 AGLTLQFVNNVIIHNIYVKHIVPGNGGLIRDSEAHIGLRTKSDGDGISLFGATNIWIDHV 268

Query: 242 SMSNCQDGLIDAVMASTAITISNCHFTHHNDVILLGASDSYSNDKIMQVTLAFNHFGKGL 301
           SM+ C DG+IDA+  STA+TISN HFT H +V+L GA D +  DK MQ+T+AFNHFGK L
Sbjct: 269 SMTRCADGMIDAIDGSTAVTISNSHFTDHQEVMLFGARDEHVIDKKMQITVAFNHFGKRL 328

Query: 302 VQRMPRCRWGFIHVVNNDYTHWLMYAIGGSHNPTIISQGNRFIAPPNPNSKEVTKRVYAS 361
            QRMPRCR+G IHVVNNDYTHW MYAIGG+ NPTIISQGNRFIAPPN  +K++TKR Y  
Sbjct: 329 EQRMPRCRYGTIHVVNNDYTHWEMYAIGGNMNPTIISQGNRFIAPPNEEAKQITKREYTP 388

Query: 362 EREWRSWNWRSEGDLMMNGAFFIQSG-------NPIRRYSKKDVIHSKPGTFVTRLTRFA 414
             EW+SWNW+SEGD  +NGA+F+QSG        P      K  I  KPGT V +LT  A
Sbjct: 389 YGEWKSWNWQSEGDYFLNGAYFVQSGKANAWSSKPKTPLPNKFTIRPKPGTMVRKLTMDA 448

BLAST of Cmc12g0335991 vs. ExPASy Swiss-Prot
Match: Q9LFP5 (Probable pectate lyase 19 OS=Arabidopsis thaliana OX=3702 GN=At5g15110 PE=2 SV=1)

HSP 1 Score: 533.9 bits (1374), Expect = 1.6e-150
Identity = 252/449 (56.12%), Postives = 327/449 (72.83%), Query Frame = 0

Query: 2   GGLCDFDEVWQKRAIEAKKASLEAYQPHPEEVTNNFNKQVH-RSLD-------------- 61
           G + + DE W +RA EA++ +L+AY   P E+ ++F+++ +  S D              
Sbjct: 24  GNISELDEYWSQRADEAREFTLQAYHSDPYEIVDHFHERHYDNSTDVTTPEEDGDAKPEE 83

Query: 62  -----------GANNTRRHLR--------KYTGPCLATNPIDRCWRCDRNWARNRKKLAD 121
                        N+TRR LR        K  GPC A+NPID+CWRC  +WA+ RKKL  
Sbjct: 84  EEKEFIEMLGSSTNSTRRSLRGKGKGKWSKLKGPCTASNPIDKCWRCRSDWAKRRKKLTR 143

Query: 122 CALGFGRGTTGGKDGKIYVVRDSSDNDLVNPKPGTLRHAVIQERPLWIIFAHDMVIRLSE 181
           C  GFG  TTGGK G+IYVV  + D D+VNPKPGTLRHAVIQ+ PLWIIF +DM IRL++
Sbjct: 144 CVRGFGHRTTGGKRGRIYVVTSNLDEDMVNPKPGTLRHAVIQKEPLWIIFKNDMSIRLNQ 203

Query: 182 ELIMTDNKTLDGRGANVHIANGGQITLQFVRNIIIHNLHIHDIKAGNGGFIRDSVSHYGY 241
           EL++  +KT+D RGANVH+A+G  IT+QFV+N+IIH LHIH I   +GG IRDSV H+G 
Sbjct: 204 ELLINSHKTIDARGANVHVAHGAGITMQFVKNVIIHGLHIHHISESSGGMIRDSVDHFGM 263

Query: 242 RTQSDGDGISMFGSSRVWIDHVSMSNCQDGLIDAVMASTAITISNCHFTHHNDVILLGAS 301
           RT++DGDG+S++GSS +W+DH+SMS CQDGLIDA++ ST ITISN HFTHHNDV+LLGA 
Sbjct: 264 RTRADGDGLSIYGSSNIWLDHISMSKCQDGLIDAIVGSTGITISNSHFTHHNDVMLLGAQ 323

Query: 302 DSYSNDKIMQVTLAFNHFGKGLVQRMPRCRWGFIHVVNNDYTHWLMYAIGGSHNPTIISQ 361
           ++   DK MQVT+A+NHFGKGLVQRMPR RWGF+HVVNNDYTHW +YAIGGS  PTI+S 
Sbjct: 324 NTNEADKHMQVTVAYNHFGKGLVQRMPRIRWGFVHVVNNDYTHWELYAIGGSQGPTILSH 383

Query: 362 GNRFIAPPN-PNSKEVTKRVYASEREWRSWNWRSEGDLMMNGAFFIQSGNPIRR--YSKK 414
           GNRFIAPP+ P+ +EVTKR YASE EW+ WNWRS+ D+ MNGA+F QSGNP  +  ++++
Sbjct: 384 GNRFIAPPHKPHYREVTKRDYASEDEWKHWNWRSDKDVFMNGAYFRQSGNPQYKCAHTRQ 443

BLAST of Cmc12g0335991 vs. ExPASy Swiss-Prot
Match: Q9SRH4 (Probable pectate lyase 7 OS=Arabidopsis thaliana OX=3702 GN=At3g01270 PE=2 SV=1)

HSP 1 Score: 531.6 bits (1368), Expect = 8.1e-150
Identity = 252/445 (56.63%), Postives = 322/445 (72.36%), Query Frame = 0

Query: 8   DEVWQKRAIEAKKASLEAYQPHPEEVTNNFNKQ------------------------VHR 67
           DE W  +A EA+K +L AY P P E+ ++F+++                        +  
Sbjct: 31  DEYWVNKANEARKHTLMAYHPDPYEIVDHFHERHYDNSTDVEGTEEEKAVASEEEDVIEM 90

Query: 68  SLDGANNTRRHL------------RKYTGPCLATNPIDRCWRCDRNWARNRKKLADCALG 127
                N+TRR L             K TGPC A+NPID+CWRC  +WAR RKKL  C  G
Sbjct: 91  ISSPTNSTRRSLTGRGKGKGKGKWSKLTGPCTASNPIDKCWRCQPDWARRRKKLVHCVRG 150

Query: 128 FGRGTTGGKDGKIYVVRDSSDNDLVNPKPGTLRHAVIQERPLWIIFAHDMVIRLSEELIM 187
           FG  TTGGK G+IYVV    D+D+VNP+PGTLRHAVIQ+ PLWI+F HDM IRLS+EL++
Sbjct: 151 FGYRTTGGKRGRIYVVTSPRDDDMVNPRPGTLRHAVIQKEPLWIVFKHDMSIRLSQELMI 210

Query: 188 TDNKTLDGRGANVHIANGGQITLQFVRNIIIHNLHIHDIKAGNGGFIRDSVSHYGYRTQS 247
           T +KT+D RGANVHIA G  IT+Q+V NIIIH LH+H I   +GG IRDS++H+G+R ++
Sbjct: 211 TSDKTIDARGANVHIAYGAGITMQYVHNIIIHGLHVHHIVKSSGGLIRDSINHFGHRGEA 270

Query: 248 DGDGISMFGSSRVWIDHVSMSNCQDGLIDAVMASTAITISNCHFTHHNDVILLGASDSYS 307
           DGDGIS+FG++ +W+DH+SMS CQDGLIDA+M STAITISN HFTHHNDV+LLGA ++  
Sbjct: 271 DGDGISIFGATNIWLDHISMSKCQDGLIDAIMGSTAITISNSHFTHHNDVMLLGAQNNNM 330

Query: 308 NDKIMQVTLAFNHFGKGLVQRMPRCRWGFIHVVNNDYTHWLMYAIGGSHNPTIISQGNRF 367
           +DK MQVT+A+NHFGKGLVQRMPR RWGF+HVVNNDYTHW +YAIGGS  PTI+S GNRF
Sbjct: 331 DDKKMQVTVAYNHFGKGLVQRMPRVRWGFVHVVNNDYTHWELYAIGGSQGPTILSHGNRF 390

Query: 368 IAPPN-PNSKEVTKRVYASEREWRSWNWRSEGDLMMNGAFFIQSGNPIRR--YSKKDVIH 414
           IAPP+  + +EVTKR YASE EW++WNWRSE D+ MN A+F QSGNP  +  +S++ +I 
Sbjct: 391 IAPPHKQHYREVTKRDYASESEWKNWNWRSEKDVFMNNAYFRQSGNPHFKCSHSRQQMIK 450

BLAST of Cmc12g0335991 vs. ExPASy TrEMBL
Match: A0A5D3BH70 (Pectate lyase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G003070 PE=3 SV=1)

HSP 1 Score: 865.5 bits (2235), Expect = 8.8e-248
Identity = 408/410 (99.51%), Postives = 409/410 (99.76%), Query Frame = 0

Query: 4   LCDFDEVWQKRAIEAKKASLEAYQPHPEEVTNNFNKQVHRSLDGANNTRRHLRKYTGPCL 63
           + DFDEVWQKRAIEAKKASLEAYQPHPEEVTNNFNKQVHRSLDGANNTRRHLRKYTGPCL
Sbjct: 30  IADFDEVWQKRAIEAKKASLEAYQPHPEEVTNNFNKQVHRSLDGANNTRRHLRKYTGPCL 89

Query: 64  ATNPIDRCWRCDRNWARNRKKLADCALGFGRGTTGGKDGKIYVVRDSSDNDLVNPKPGTL 123
           ATNPIDRCWRCDRNWARNRKKLADCALGFGRGTTGGKDGKIYVVRDSSDNDLVNPKPGTL
Sbjct: 90  ATNPIDRCWRCDRNWARNRKKLADCALGFGRGTTGGKDGKIYVVRDSSDNDLVNPKPGTL 149

Query: 124 RHAVIQERPLWIIFAHDMVIRLSEELIMTDNKTLDGRGANVHIANGGQITLQFVRNIIIH 183
           RHAVIQERPLWIIFAHDMVIRLSEELIMTDNKTLDGRGANVHIANGGQITLQFVRNIIIH
Sbjct: 150 RHAVIQERPLWIIFAHDMVIRLSEELIMTDNKTLDGRGANVHIANGGQITLQFVRNIIIH 209

Query: 184 NLHIHDIKAGNGGFIRDSVSHYGYRTQSDGDGISMFGSSRVWIDHVSMSNCQDGLIDAVM 243
           NLHIHDIKAGNGGFIRDSVSHYGYRTQSDGDGISMFGSSRVWIDHVSMSNCQDGLIDAVM
Sbjct: 210 NLHIHDIKAGNGGFIRDSVSHYGYRTQSDGDGISMFGSSRVWIDHVSMSNCQDGLIDAVM 269

Query: 244 ASTAITISNCHFTHHNDVILLGASDSYSNDKIMQVTLAFNHFGKGLVQRMPRCRWGFIHV 303
           ASTAITISNCHFTHHNDVILLGASDSYSNDKIMQVTLAFNHFGKGLVQRMPRCRWGFIHV
Sbjct: 270 ASTAITISNCHFTHHNDVILLGASDSYSNDKIMQVTLAFNHFGKGLVQRMPRCRWGFIHV 329

Query: 304 VNNDYTHWLMYAIGGSHNPTIISQGNRFIAPPNPNSKEVTKRVYASEREWRSWNWRSEGD 363
           VNNDYTHWLMYAIGGSHNPTIISQGNRFIAPPNPNSKEVTKRVYASEREWRSWNWRSEGD
Sbjct: 330 VNNDYTHWLMYAIGGSHNPTIISQGNRFIAPPNPNSKEVTKRVYASEREWRSWNWRSEGD 389

Query: 364 LMMNGAFFIQSGNPIRRYSKKDVIHSKPGTFVTRLTRFAGPLKCKMNQPC 414
           LMMNGAFFIQSGNPIRRYSKKDVIHSKPGTFVTRLTRFAGPLKCKMNQPC
Sbjct: 390 LMMNGAFFIQSGNPIRRYSKKDVIHSKPGTFVTRLTRFAGPLKCKMNQPC 439

BLAST of Cmc12g0335991 vs. ExPASy TrEMBL
Match: A0A0A0LR32 (Pectate lyase OS=Cucumis sativus OX=3659 GN=Csa_1G045510 PE=3 SV=1)

HSP 1 Score: 828.6 bits (2139), Expect = 1.2e-236
Identity = 387/410 (94.39%), Postives = 399/410 (97.32%), Query Frame = 0

Query: 4   LCDFDEVWQKRAIEAKKASLEAYQPHPEEVTNNFNKQVHRSLDGANNTRRHLRKYTGPCL 63
           + DFDEVWQKRAIEA+KAS EAY+PHPEE TNNFNKQVHRSLDG NNTRRHLRKYTGPCL
Sbjct: 30  IADFDEVWQKRAIEAQKASFEAYEPHPEEETNNFNKQVHRSLDGGNNTRRHLRKYTGPCL 89

Query: 64  ATNPIDRCWRCDRNWARNRKKLADCALGFGRGTTGGKDGKIYVVRDSSDNDLVNPKPGTL 123
           ATNPIDRCWRCDRNWARNRKKLADCALGFGR TTGGKDGKIYVVRDSSDNDLVNPKPGTL
Sbjct: 90  ATNPIDRCWRCDRNWARNRKKLADCALGFGRRTTGGKDGKIYVVRDSSDNDLVNPKPGTL 149

Query: 124 RHAVIQERPLWIIFAHDMVIRLSEELIMTDNKTLDGRGANVHIANGGQITLQFVRNIIIH 183
           RHAVIQERPLWIIFAHDMVIRLSEELI+TD+KTLDGRGANVHIANGGQITLQFV+NIIIH
Sbjct: 150 RHAVIQERPLWIIFAHDMVIRLSEELIVTDDKTLDGRGANVHIANGGQITLQFVKNIIIH 209

Query: 184 NLHIHDIKAGNGGFIRDSVSHYGYRTQSDGDGISMFGSSRVWIDHVSMSNCQDGLIDAVM 243
           NLHIHDIKAGNGG IRDSVSHYG+RT+SDGDGISMFG+SRVWIDHVSMSNCQDGLIDAVM
Sbjct: 210 NLHIHDIKAGNGGMIRDSVSHYGFRTRSDGDGISMFGASRVWIDHVSMSNCQDGLIDAVM 269

Query: 244 ASTAITISNCHFTHHNDVILLGASDSYSNDKIMQVTLAFNHFGKGLVQRMPRCRWGFIHV 303
           ASTAITISNCHFTHHNDVILLGAS+ YSND+IMQVTLAFNHFGKGLVQRMPRCRWGFIHV
Sbjct: 270 ASTAITISNCHFTHHNDVILLGASNGYSNDQIMQVTLAFNHFGKGLVQRMPRCRWGFIHV 329

Query: 304 VNNDYTHWLMYAIGGSHNPTIISQGNRFIAPPNPNSKEVTKRVYASEREWRSWNWRSEGD 363
           VNNDYTHWLMYAIGGSHNPTIISQGNRFIAPPNPN KEVTKRVYA E EWRSWNWRSEGD
Sbjct: 330 VNNDYTHWLMYAIGGSHNPTIISQGNRFIAPPNPNCKEVTKRVYAPESEWRSWNWRSEGD 389

Query: 364 LMMNGAFFIQSGNPIRRYSKKDVIHSKPGTFVTRLTRFAGPLKCKMNQPC 414
           LMMNGAFFIQSGNPI+RYSKKDVIHSKPGTFVTRLTRFAGPLKCK NQPC
Sbjct: 390 LMMNGAFFIQSGNPIKRYSKKDVIHSKPGTFVTRLTRFAGPLKCKKNQPC 439

BLAST of Cmc12g0335991 vs. ExPASy TrEMBL
Match: A0A1S3BF07 (Pectate lyase OS=Cucumis melo OX=3656 GN=LOC103488907 PE=3 SV=1)

HSP 1 Score: 807.4 bits (2084), Expect = 2.8e-230
Identity = 380/382 (99.48%), Postives = 381/382 (99.74%), Query Frame = 0

Query: 32  EVTNNFNKQVHRSLDGANNTRRHLRKYTGPCLATNPIDRCWRCDRNWARNRKKLADCALG 91
           + TNNFNKQVHRSLDGANNTRRHLRKYTGPCLATNPIDRCWRCDRNWARNRKKLADCALG
Sbjct: 19  DFTNNFNKQVHRSLDGANNTRRHLRKYTGPCLATNPIDRCWRCDRNWARNRKKLADCALG 78

Query: 92  FGRGTTGGKDGKIYVVRDSSDNDLVNPKPGTLRHAVIQERPLWIIFAHDMVIRLSEELIM 151
           FGRGTTGGKDGKIYVVRDSSDNDLVNPKPGTLRHAVIQERPLWIIFAHDMVIRLSEELIM
Sbjct: 79  FGRGTTGGKDGKIYVVRDSSDNDLVNPKPGTLRHAVIQERPLWIIFAHDMVIRLSEELIM 138

Query: 152 TDNKTLDGRGANVHIANGGQITLQFVRNIIIHNLHIHDIKAGNGGFIRDSVSHYGYRTQS 211
           TDNKTLDGRGANVHIANGGQITLQFVRNIIIHNLHIHDIKAGNGGFIRDSVSHYGYRTQS
Sbjct: 139 TDNKTLDGRGANVHIANGGQITLQFVRNIIIHNLHIHDIKAGNGGFIRDSVSHYGYRTQS 198

Query: 212 DGDGISMFGSSRVWIDHVSMSNCQDGLIDAVMASTAITISNCHFTHHNDVILLGASDSYS 271
           DGDGISMFGSSRVWIDHVSMSNCQDGLIDAVMASTAITISNCHFTHHNDVILLGASDSYS
Sbjct: 199 DGDGISMFGSSRVWIDHVSMSNCQDGLIDAVMASTAITISNCHFTHHNDVILLGASDSYS 258

Query: 272 NDKIMQVTLAFNHFGKGLVQRMPRCRWGFIHVVNNDYTHWLMYAIGGSHNPTIISQGNRF 331
           NDKIMQVTLAFNHFGKGLVQRMPRCRWGFIHVVNNDYTHWLMYAIGGSHNPTIISQGNRF
Sbjct: 259 NDKIMQVTLAFNHFGKGLVQRMPRCRWGFIHVVNNDYTHWLMYAIGGSHNPTIISQGNRF 318

Query: 332 IAPPNPNSKEVTKRVYASEREWRSWNWRSEGDLMMNGAFFIQSGNPIRRYSKKDVIHSKP 391
           IAPPNPNSKEVTKRVYASEREWRSWNWRSEGDLMMNGAFFIQSGNPIRRYSKKDVIHSKP
Sbjct: 319 IAPPNPNSKEVTKRVYASEREWRSWNWRSEGDLMMNGAFFIQSGNPIRRYSKKDVIHSKP 378

Query: 392 GTFVTRLTRFAGPLKCKMNQPC 414
           GTFVTRLTRFAGPLKCKMNQPC
Sbjct: 379 GTFVTRLTRFAGPLKCKMNQPC 400

BLAST of Cmc12g0335991 vs. ExPASy TrEMBL
Match: A0A6J1K214 (Pectate lyase OS=Cucurbita maxima OX=3661 GN=LOC111489953 PE=3 SV=1)

HSP 1 Score: 778.1 bits (2008), Expect = 1.8e-221
Identity = 356/410 (86.83%), Postives = 390/410 (95.12%), Query Frame = 0

Query: 4   LCDFDEVWQKRAIEAKKASLEAYQPHPEEVTNNFNKQVHRSLDGANNTRRHLRKYTGPCL 63
           + DFDEVWQ+RA EAKKA+LEAYQPHPEEVT NFN+ V+  LD ANNTRRHLRKY GPCL
Sbjct: 31  IADFDEVWQQRAQEAKKAALEAYQPHPEEVTENFNEHVNGDLDDANNTRRHLRKYNGPCL 90

Query: 64  ATNPIDRCWRCDRNWARNRKKLADCALGFGRGTTGGKDGKIYVVRDSSDNDLVNPKPGTL 123
           ATNPIDRCWRCD+NWA+NRKKLADCALGFGRGTTGGK+G+ YVV+DSSDND+++PKPGTL
Sbjct: 91  ATNPIDRCWRCDKNWAKNRKKLADCALGFGRGTTGGKNGRFYVVQDSSDNDMLHPKPGTL 150

Query: 124 RHAVIQERPLWIIFAHDMVIRLSEELIMTDNKTLDGRGANVHIANGGQITLQFVRNIIIH 183
           R+AVIQ+RPLWI FAHDMVIRL+EELIMTDNKTLDGRGANVHIANGGQITLQFV+NIIIH
Sbjct: 151 RYAVIQDRPLWITFAHDMVIRLAEELIMTDNKTLDGRGANVHIANGGQITLQFVKNIIIH 210

Query: 184 NLHIHDIKAGNGGFIRDSVSHYGYRTQSDGDGISMFGSSRVWIDHVSMSNCQDGLIDAVM 243
           NLHIHDIKAGNGG IRDSV+HYG RT+SDGDGIS+FG+S VWIDHVSMSNCQDGLIDAVM
Sbjct: 211 NLHIHDIKAGNGGLIRDSVNHYGIRTRSDGDGISIFGASNVWIDHVSMSNCQDGLIDAVM 270

Query: 244 ASTAITISNCHFTHHNDVILLGASDSYSNDKIMQVTLAFNHFGKGLVQRMPRCRWGFIHV 303
           ASTAITISNCHF HHNDVILLGASDS+S+D+IMQVTLAFNHFGKGLVQRMPRCRWGF+HV
Sbjct: 271 ASTAITISNCHFAHHNDVILLGASDSHSDDQIMQVTLAFNHFGKGLVQRMPRCRWGFVHV 330

Query: 304 VNNDYTHWLMYAIGGSHNPTIISQGNRFIAPPNPNSKEVTKRVYASEREWRSWNWRSEGD 363
           VNNDYTHWLMYAIGGS NPTIISQGNRFIAPP+PN KEVTKRVYA E +W++WNWRSEGD
Sbjct: 331 VNNDYTHWLMYAIGGSRNPTIISQGNRFIAPPDPNCKEVTKRVYAPESDWKTWNWRSEGD 390

Query: 364 LMMNGAFFIQSGNPIRRYSKKDVIHSKPGTFVTRLTRFAGPLKCKMNQPC 414
           L+MNGAFF++SGNPIR+YSKKDVIH+KPGTFVTRLTRFAGPLKC  NQPC
Sbjct: 391 LLMNGAFFVESGNPIRKYSKKDVIHAKPGTFVTRLTRFAGPLKCTRNQPC 440

BLAST of Cmc12g0335991 vs. ExPASy TrEMBL
Match: A0A6J1GTX4 (Pectate lyase OS=Cucurbita moschata OX=3662 GN=LOC111457080 PE=3 SV=1)

HSP 1 Score: 775.4 bits (2001), Expect = 1.2e-220
Identity = 355/411 (86.37%), Postives = 390/411 (94.89%), Query Frame = 0

Query: 3   GLCDFDEVWQKRAIEAKKASLEAYQPHPEEVTNNFNKQVHRSLDGANNTRRHLRKYTGPC 62
           G+ DFDEVWQ+RA EAKKA+LEAYQP PEEVT +FN+ V+  LD ANNTRRHLRKY GPC
Sbjct: 31  GIADFDEVWQQRAQEAKKAALEAYQPRPEEVTESFNEHVNGDLDDANNTRRHLRKYNGPC 90

Query: 63  LATNPIDRCWRCDRNWARNRKKLADCALGFGRGTTGGKDGKIYVVRDSSDNDLVNPKPGT 122
           LATNPIDRCWRCD+NW++NRKKLADCALGFGRGTTGGK+G+ Y+V+DSSDND+++PKPGT
Sbjct: 91  LATNPIDRCWRCDKNWSKNRKKLADCALGFGRGTTGGKNGRFYIVQDSSDNDMLHPKPGT 150

Query: 123 LRHAVIQERPLWIIFAHDMVIRLSEELIMTDNKTLDGRGANVHIANGGQITLQFVRNIII 182
           LR+AVIQ+RPLWI FAHDMVIRL+EELIMTDNKTLDGRGANVHIANGGQITLQFV+NIII
Sbjct: 151 LRYAVIQDRPLWITFAHDMVIRLAEELIMTDNKTLDGRGANVHIANGGQITLQFVKNIII 210

Query: 183 HNLHIHDIKAGNGGFIRDSVSHYGYRTQSDGDGISMFGSSRVWIDHVSMSNCQDGLIDAV 242
           HNLHIHDIKAGNGG IRDSV+HYG RT+SDGDGISMFG+S VWIDHVSMSNCQDGLIDAV
Sbjct: 211 HNLHIHDIKAGNGGLIRDSVNHYGIRTRSDGDGISMFGASNVWIDHVSMSNCQDGLIDAV 270

Query: 243 MASTAITISNCHFTHHNDVILLGASDSYSNDKIMQVTLAFNHFGKGLVQRMPRCRWGFIH 302
           MASTAITISNCHF HHNDVILLGASDS+S+D+IMQVTLAFNHFGKGLVQRMPRCRWGF+H
Sbjct: 271 MASTAITISNCHFAHHNDVILLGASDSHSDDQIMQVTLAFNHFGKGLVQRMPRCRWGFVH 330

Query: 303 VVNNDYTHWLMYAIGGSHNPTIISQGNRFIAPPNPNSKEVTKRVYASEREWRSWNWRSEG 362
           VVNNDYTHWLMYAIGGS NPTIISQGNRFIAPP+PN KEVTKRVYA E EW++WNWRSEG
Sbjct: 331 VVNNDYTHWLMYAIGGSRNPTIISQGNRFIAPPDPNCKEVTKRVYAPESEWKTWNWRSEG 390

Query: 363 DLMMNGAFFIQSGNPIRRYSKKDVIHSKPGTFVTRLTRFAGPLKCKMNQPC 414
           DL+MNGAFF+QSGNPIR+YSKKDVIH+KPGTFVTRLTRFAGPLKC  N+PC
Sbjct: 391 DLLMNGAFFVQSGNPIRKYSKKDVIHAKPGTFVTRLTRFAGPLKCIRNKPC 441

BLAST of Cmc12g0335991 vs. TAIR 10
Match: AT1G14420.1 (Pectate lyase family protein )

HSP 1 Score: 547.4 bits (1409), Expect = 1.0e-155
Identity = 258/431 (59.86%), Postives = 317/431 (73.55%), Query Frame = 0

Query: 2   GGLCDFDEVWQKRAIEAKKASLEAYQPHPEEVTNNFNKQVHRSLDGA---NNTRRHL--- 61
           G +  FD  W +R  +A K ++ +Y PHP  VTN+FN  V+ ++D +   N+TRR L   
Sbjct: 29  GNVAVFDSYWTQRQSDALKQTIGSYDPHPLNVTNHFNYHVNIAVDASESRNDTRRELTQV 88

Query: 62  ------RKYTGPCLATNPIDRCWRCDRNWARNRKKLADCALGFGRGTTGGKDGKIYVVRD 121
                  K +G CLA NPID CWRCDRNWA NRKKLADC LGFGR TTGGKDG IYVV+D
Sbjct: 89  RSGRKTHKSSGKCLAYNPIDNCWRCDRNWANNRKKLADCVLGFGRRTTGGKDGPIYVVKD 148

Query: 122 SSDNDLVNPKPGTLRHAVIQERPLWIIFAHDMVIRLSEELIMTDNKTLDGRGANVHIANG 181
           +SDNDL+NPKPGTLRHAV ++ PLWIIFA  M+I+L +EL++T +KT+DGRGA V+I  G
Sbjct: 149 ASDNDLINPKPGTLRHAVTRDGPLWIIFARSMIIKLQQELMITSDKTIDGRGARVYIMEG 208

Query: 182 GQITLQFVRNIIIHNLHIHDIKAGNGGFIRDSVSHYGYRTQSDGDGISMFGSSRVWIDHV 241
             +TLQFV N+IIHN+++  I  GNGG IRDS +H G RT+SDGDGIS+FG++ +WIDHV
Sbjct: 209 AGLTLQFVNNVIIHNIYVKHIVPGNGGLIRDSEAHIGLRTKSDGDGISLFGATNIWIDHV 268

Query: 242 SMSNCQDGLIDAVMASTAITISNCHFTHHNDVILLGASDSYSNDKIMQVTLAFNHFGKGL 301
           SM+ C DG+IDA+  STA+TISN HFT H +V+L GA D +  DK MQ+T+AFNHFGK L
Sbjct: 269 SMTRCADGMIDAIDGSTAVTISNSHFTDHQEVMLFGARDEHVIDKKMQITVAFNHFGKRL 328

Query: 302 VQRMPRCRWGFIHVVNNDYTHWLMYAIGGSHNPTIISQGNRFIAPPNPNSKEVTKRVYAS 361
            QRMPRCR+G IHVVNNDYTHW MYAIGG+ NPTIISQGNRFIAPPN  +K++TKR Y  
Sbjct: 329 EQRMPRCRYGTIHVVNNDYTHWEMYAIGGNMNPTIISQGNRFIAPPNEEAKQITKREYTP 388

Query: 362 EREWRSWNWRSEGDLMMNGAFFIQSG-------NPIRRYSKKDVIHSKPGTFVTRLTRFA 414
             EW+SWNW+SEGD  +NGA+F+QSG        P      K  I  KPGT V +LT  A
Sbjct: 389 YGEWKSWNWQSEGDYFLNGAYFVQSGKANAWSSKPKTPLPNKFTIRPKPGTMVRKLTMDA 448

BLAST of Cmc12g0335991 vs. TAIR 10
Match: AT5G15110.1 (Pectate lyase family protein )

HSP 1 Score: 533.5 bits (1373), Expect = 1.5e-151
Identity = 251/449 (55.90%), Postives = 327/449 (72.83%), Query Frame = 0

Query: 2   GGLCDFDEVWQKRAIEAKKASLEAYQPHPEEVTNNFNKQVH-RSLD-------------- 61
           G + + DE W +RA EA++ +L+AY   P E+ ++F+++ +  S D              
Sbjct: 24  GNISELDEYWSQRADEAREFTLQAYHSDPYEIVDHFHERHYDNSTDVTTPEEDGDAKPEE 83

Query: 62  -----------GANNTRRHLR--------KYTGPCLATNPIDRCWRCDRNWARNRKKLAD 121
                        N+TRR LR        K  GPC A+NPID+CWRC  +WA+ RKKL  
Sbjct: 84  EEKEFIEMLGSSTNSTRRSLRGKGKGKWSKLKGPCTASNPIDKCWRCRSDWAKRRKKLTR 143

Query: 122 CALGFGRGTTGGKDGKIYVVRDSSDNDLVNPKPGTLRHAVIQERPLWIIFAHDMVIRLSE 181
           C  GFG  TTGGK G+IYVV  + D D+VNPKPGTLRHAVIQ+ PLWIIF +DM IRL++
Sbjct: 144 CVRGFGHRTTGGKRGRIYVVTSNLDEDMVNPKPGTLRHAVIQKEPLWIIFKNDMSIRLNQ 203

Query: 182 ELIMTDNKTLDGRGANVHIANGGQITLQFVRNIIIHNLHIHDIKAGNGGFIRDSVSHYGY 241
           EL++  +KT+D RGANVH+A+G  IT+QFV+N++IH LHIH I   +GG IRDSV H+G 
Sbjct: 204 ELLINSHKTIDARGANVHVAHGAGITMQFVKNVVIHGLHIHHISESSGGMIRDSVDHFGM 263

Query: 242 RTQSDGDGISMFGSSRVWIDHVSMSNCQDGLIDAVMASTAITISNCHFTHHNDVILLGAS 301
           RT++DGDG+S++GSS +W+DH+SMS CQDGLIDA++ ST ITISN HFTHHNDV+LLGA 
Sbjct: 264 RTRADGDGLSIYGSSNIWLDHISMSKCQDGLIDAIVGSTGITISNSHFTHHNDVMLLGAQ 323

Query: 302 DSYSNDKIMQVTLAFNHFGKGLVQRMPRCRWGFIHVVNNDYTHWLMYAIGGSHNPTIISQ 361
           ++   DK MQVT+A+NHFGKGLVQRMPR RWGF+HVVNNDYTHW +YAIGGS  PTI+S 
Sbjct: 324 NTNEADKHMQVTVAYNHFGKGLVQRMPRIRWGFVHVVNNDYTHWELYAIGGSQGPTILSH 383

Query: 362 GNRFIAPPN-PNSKEVTKRVYASEREWRSWNWRSEGDLMMNGAFFIQSGNPIRR--YSKK 414
           GNRFIAPP+ P+ +EVTKR YASE EW+ WNWRS+ D+ MNGA+F QSGNP  +  ++++
Sbjct: 384 GNRFIAPPHKPHYREVTKRDYASEDEWKHWNWRSDKDVFMNGAYFRQSGNPQYKCAHTRQ 443

BLAST of Cmc12g0335991 vs. TAIR 10
Match: AT3G01270.1 (Pectate lyase family protein )

HSP 1 Score: 531.6 bits (1368), Expect = 5.8e-151
Identity = 252/445 (56.63%), Postives = 322/445 (72.36%), Query Frame = 0

Query: 8   DEVWQKRAIEAKKASLEAYQPHPEEVTNNFNKQ------------------------VHR 67
           DE W  +A EA+K +L AY P P E+ ++F+++                        +  
Sbjct: 31  DEYWVNKANEARKHTLMAYHPDPYEIVDHFHERHYDNSTDVEGTEEEKAVASEEEDVIEM 90

Query: 68  SLDGANNTRRHL------------RKYTGPCLATNPIDRCWRCDRNWARNRKKLADCALG 127
                N+TRR L             K TGPC A+NPID+CWRC  +WAR RKKL  C  G
Sbjct: 91  ISSPTNSTRRSLTGRGKGKGKGKWSKLTGPCTASNPIDKCWRCQPDWARRRKKLVHCVRG 150

Query: 128 FGRGTTGGKDGKIYVVRDSSDNDLVNPKPGTLRHAVIQERPLWIIFAHDMVIRLSEELIM 187
           FG  TTGGK G+IYVV    D+D+VNP+PGTLRHAVIQ+ PLWI+F HDM IRLS+EL++
Sbjct: 151 FGYRTTGGKRGRIYVVTSPRDDDMVNPRPGTLRHAVIQKEPLWIVFKHDMSIRLSQELMI 210

Query: 188 TDNKTLDGRGANVHIANGGQITLQFVRNIIIHNLHIHDIKAGNGGFIRDSVSHYGYRTQS 247
           T +KT+D RGANVHIA G  IT+Q+V NIIIH LH+H I   +GG IRDS++H+G+R ++
Sbjct: 211 TSDKTIDARGANVHIAYGAGITMQYVHNIIIHGLHVHHIVKSSGGLIRDSINHFGHRGEA 270

Query: 248 DGDGISMFGSSRVWIDHVSMSNCQDGLIDAVMASTAITISNCHFTHHNDVILLGASDSYS 307
           DGDGIS+FG++ +W+DH+SMS CQDGLIDA+M STAITISN HFTHHNDV+LLGA ++  
Sbjct: 271 DGDGISIFGATNIWLDHISMSKCQDGLIDAIMGSTAITISNSHFTHHNDVMLLGAQNNNM 330

Query: 308 NDKIMQVTLAFNHFGKGLVQRMPRCRWGFIHVVNNDYTHWLMYAIGGSHNPTIISQGNRF 367
           +DK MQVT+A+NHFGKGLVQRMPR RWGF+HVVNNDYTHW +YAIGGS  PTI+S GNRF
Sbjct: 331 DDKKMQVTVAYNHFGKGLVQRMPRVRWGFVHVVNNDYTHWELYAIGGSQGPTILSHGNRF 390

Query: 368 IAPPN-PNSKEVTKRVYASEREWRSWNWRSEGDLMMNGAFFIQSGNPIRR--YSKKDVIH 414
           IAPP+  + +EVTKR YASE EW++WNWRSE D+ MN A+F QSGNP  +  +S++ +I 
Sbjct: 391 IAPPHKQHYREVTKRDYASESEWKNWNWRSEKDVFMNNAYFRQSGNPHFKCSHSRQQMIK 450

BLAST of Cmc12g0335991 vs. TAIR 10
Match: AT2G02720.1 (Pectate lyase family protein )

HSP 1 Score: 513.1 bits (1320), Expect = 2.1e-145
Identity = 249/432 (57.64%), Postives = 313/432 (72.45%), Query Frame = 0

Query: 4   LCDFDEVWQKRAIEAKKASLEAYQPHPEEVTNNFNKQVHRSLDG---ANNTRRHLR---- 63
           + ++DE W +R   A + +LE+Y P+PE VT++FN     +++     N TRR LR    
Sbjct: 27  IAEYDEYWTQRQTNALRETLESYDPNPENVTDHFNYHAALAMETTGIVNETRRDLRQVGR 86

Query: 64  -----KYTGPCLATNPIDRCWRCDRNWARNRKKLADCALGFGRGTTGGKDGKIYVVRDSS 123
                +  G   + N ID+CWR D+NW +NRKKLADC LGFGR TTGGK+G IYVV D S
Sbjct: 87  GKKTTRRGGRFESLNAIDKCWRGDKNWDKNRKKLADCVLGFGRKTTGGKNGPIYVVTDPS 146

Query: 124 DNDLVNPKPGTLRHAVIQERPLWIIFAHDMVIRLSEELIMTDNKTLDGRGANVHIANGGQ 183
           DNDL+ PKPGT+RHAV ++RPLWIIFA  M+I+L +ELI+T++KT+DGRGA ++I  G  
Sbjct: 147 DNDLLKPKPGTIRHAVTRDRPLWIIFARSMIIKLQQELIITNDKTIDGRGAKIYITGGAG 206

Query: 184 ITLQFVRNIIIHNLHIHDIKAGNGGFIRDSVSHYGYRTQSDGDGISMFGSSRVWIDHVSM 243
           +TLQFVRN+IIHN+HI  IK G GG I DS  H+G RT SDGDGI++FG++ VWIDHVSM
Sbjct: 207 LTLQFVRNVIIHNIHIKQIKRGAGGLIIDSEQHFGLRTVSDGDGINIFGATNVWIDHVSM 266

Query: 244 SNCQDGLIDAVMASTAITISNCHFTHHNDVILLGASDSYSNDKIMQVTLAFNHFGKGLVQ 303
           ++C DG+IDA+M STAITISN HFT H++V+L G ++    DK MQ+T+AFNHFGK L Q
Sbjct: 267 TDCSDGMIDAIMGSTAITISNSHFTDHDEVMLFGGTNKDVIDKKMQITVAFNHFGKRLKQ 326

Query: 304 RMPRCRWGFIHVVNNDYTHWLMYAIGGSHNPTIISQGNRFIAPPNPNSKEVTKRVYASER 363
           RMPR R+G +HVVNNDYTHW MYAIGG+ NPTIISQGNRFIAPP  +SK+VTKR Y    
Sbjct: 327 RMPRVRFGLVHVVNNDYTHWEMYAIGGNMNPTIISQGNRFIAPPIEDSKQVTKREYTPYP 386

Query: 364 EWRSWNWRSEGDLMMNGAFFIQSG----------NPIRRYSKKDVIHSKPGTFVTRLTRF 414
           EW+SWNW+SE D  +NGA+F+QSG          NPI R   K  I  +PGT V RLT+ 
Sbjct: 387 EWKSWNWQSEKDYFLNGAYFVQSGKANAWSATPKNPIPR---KFAIRPQPGTKVRRLTKD 446

BLAST of Cmc12g0335991 vs. TAIR 10
Match: AT5G48900.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 497.7 bits (1280), Expect = 9.2e-141
Identity = 239/407 (58.72%), Postives = 294/407 (72.24%), Query Frame = 0

Query: 8   DEVWQKRAIEAKKASLEAYQPHPEEVTNNFNKQVHRSLDGANNTRRHLRKYTGPCLATNP 67
           +E W + A+E           +PEEV    +  +  S       RR L  ++  C   NP
Sbjct: 29  NETWHEHAVE-----------NPEEVAAMVDMSIRNS-----TARRRLGYFS--CSTGNP 88

Query: 68  IDRCWRCDRNWARNRKKLADCALGFGRGTTGGKDGKIYVVRDSSDNDLVNPKPGTLRHAV 127
           ID CWRCDR W   RK LA+CA+GFGR   GG+DG+ YVV D +D++ VNPKPGTLRHAV
Sbjct: 89  IDDCWRCDRRWQSRRKHLANCAIGFGRNAIGGRDGRYYVVSDPNDDNPVNPKPGTLRHAV 148

Query: 128 IQERPLWIIFAHDMVIRLSEELIMTDNKTLDGRGANVHIANGGQITLQFVRNIIIHNLHI 187
           IQE PLWI+F  DMVI L EELIM   KT+DGRG NVHIANG  IT+QFV NIIIH +HI
Sbjct: 149 IQEEPLWIVFKRDMVITLKEELIMNSFKTIDGRGVNVHIANGACITIQFVTNIIIHGIHI 208

Query: 188 HDIKAGNGGFIRDSVSHYGYRTQSDGDGISMFGSSRVWIDHVSMSNCQDGLIDAVMASTA 247
           HD +      +R S SHYG+RT +DGDGIS+FGSS +WIDH S+SNC DGLIDAVMASTA
Sbjct: 209 HDCRPTGNAMVRSSPSHYGWRTMADGDGISIFGSSHIWIDHNSLSNCADGLIDAVMASTA 268

Query: 248 ITISNCHFTHHNDVILLGASDSYSNDKIMQVTLAFNHFGKGLVQRMPRCRWGFIHVVNND 307
           ITISN +FTHHN+V+LLG SD+Y+ DK+MQVT+A+NHFG+GL+QRMPRCR G+ HVVNND
Sbjct: 269 ITISNNYFTHHNEVMLLGHSDTYTRDKVMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNND 328

Query: 308 YTHWLMYAIGGSHNPTIISQGNRFIAPPNPNSKEVTKRVYASEREWRSWNWRSEGDLMMN 367
           YTHW MYAIGGS +PTI SQGNR++AP N  +KEVTKR YA + +WR WNWRSEGDL +N
Sbjct: 329 YTHWEMYAIGGSASPTINSQGNRYLAPRNRFAKEVTKRDYAGQWQWRHWNWRSEGDLFLN 388

Query: 368 GAFFIQSGNPI-RRYSKKDVIHSKPGTFVTRLTRFAGPLKCKMNQPC 414
           GAFF +SG+ +   Y++   + +K  + V  +T  AG L C+  + C
Sbjct: 389 GAFFTRSGSGLGASYARASSLAAKSSSLVGVITYNAGALNCRGGRRC 417

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
TYJ99102.11.8e-24799.51pectate lyase-like isoform X2 [Cucumis melo var. makuwa][more]
XP_011651190.11.4e-23994.92pectate lyase isoform X2 [Cucumis sativus][more]
XP_004152537.22.4e-23694.39pectate lyase isoform X1 [Cucumis sativus] >KGN64253.1 hypothetical protein Csa_... [more]
XP_031735989.13.5e-23593.95pectate lyase isoform X3 [Cucumis sativus][more]
XP_038894610.13.7e-23292.20pectate lyase-like isoform X2 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
P409734.7e-16665.78Pectate lyase OS=Lilium longiflorum OX=4690 PE=2 SV=1[more]
P157223.8e-15562.21Probable pectate lyase P59 OS=Solanum lycopersicum OX=4081 GN=LAT59 PE=2 SV=1[more]
Q9M9S21.4e-15459.86Probable pectate lyase 3 OS=Arabidopsis thaliana OX=3702 GN=AT59 PE=2 SV=1[more]
Q9LFP51.6e-15056.12Probable pectate lyase 19 OS=Arabidopsis thaliana OX=3702 GN=At5g15110 PE=2 SV=1[more]
Q9SRH48.1e-15056.63Probable pectate lyase 7 OS=Arabidopsis thaliana OX=3702 GN=At3g01270 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A5D3BH708.8e-24899.51Pectate lyase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G003070... [more]
A0A0A0LR321.2e-23694.39Pectate lyase OS=Cucumis sativus OX=3659 GN=Csa_1G045510 PE=3 SV=1[more]
A0A1S3BF072.8e-23099.48Pectate lyase OS=Cucumis melo OX=3656 GN=LOC103488907 PE=3 SV=1[more]
A0A6J1K2141.8e-22186.83Pectate lyase OS=Cucurbita maxima OX=3661 GN=LOC111489953 PE=3 SV=1[more]
A0A6J1GTX41.2e-22086.37Pectate lyase OS=Cucurbita moschata OX=3662 GN=LOC111457080 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT1G14420.11.0e-15559.86Pectate lyase family protein [more]
AT5G15110.11.5e-15155.90Pectate lyase family protein [more]
AT3G01270.15.8e-15156.63Pectate lyase family protein [more]
AT2G02720.12.1e-14557.64Pectate lyase family protein [more]
AT5G48900.19.2e-14158.72Pectin lyase-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Melon (Charmono) v1.1
Date Performed: 2022-10-13
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR018082AmbAllergenPRINTSPR00807AMBALLERGENcoord: 88..105
score: 64.81
coord: 112..137
score: 69.05
coord: 292..311
score: 76.19
coord: 314..333
score: 80.48
coord: 148..164
score: 62.18
coord: 212..233
score: 61.9
coord: 356..380
score: 51.05
IPR002022Pectate lyaseSMARTSM00656amb_allcoord: 140..337
e-value: 3.5E-100
score: 348.8
IPR002022Pectate lyasePFAMPF00544Pectate_lyase_4coord: 151..331
e-value: 5.1E-21
score: 75.2
IPR012334Pectin lyase foldGENE3D2.160.20.10coord: 65..406
e-value: 1.6E-115
score: 387.7
IPR007524Pectate lyase, N-terminalPFAMPF04431Pec_lyase_Ncoord: 6..55
e-value: 1.6E-21
score: 76.3
NoneNo IPR availablePANTHERPTHR31683:SF146PECTATE LYASEcoord: 4..413
IPR045032Pectin lyase familyPANTHERPTHR31683PECTATE LYASE 18-RELATEDcoord: 4..413
IPR011050Pectin lyase fold/virulence factorSUPERFAMILY51126Pectin lyase-likecoord: 54..407

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cmc12g0335991.1Cmc12g0335991.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0045490 pectin catabolic process
cellular_component GO:0016020 membrane
molecular_function GO:0046872 metal ion binding
molecular_function GO:0030570 pectate lyase activity