Cmc12g0334251 (gene) Melon (Charmono) v1.1

Overview
NameCmc12g0334251
Typegene
OrganismCucumis melo L. var. cantalupensis cv. Charmono (Melon (Charmono) v1.1)
Description4-coumarate-CoA ligase
LocationCMiso1.1chr12: 23788239 .. 23791829 (+)
RNA-Seq ExpressionCmc12g0334251
SyntenyCmc12g0334251
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TCTTCTAATTTAATTAATAAGCACTTACAAAAAGTGAATTAGGAGTCCATTCATCATTCCATAATCAAAAAATATATTTTTTTTTCTTCAAGTAAAACCGTTAGTTAATATCTAAAAATTACATATAAAAAGAAAAGTTTGCGTTAATTAGTACCAAACATTTTTCTAATAAATTATAAAATAATCTGAATCAGTCAGCATCACTTTCTTCGAGTTCACTTCCATGGCAGCTCGGAACTCTAACTTCAACGCCGCCCCCCATTCCATTGATCTTCGAAGCGGTTTCTGTCCTCTGACGAAGATTTTCCACAGCCTTCGACCACCGCTCTCACTTCCACCATTATCTCAGCCTCTCTCCGTCACCCAACACGCCTTTTCTCTCCTTCAATCATCTCCTCTCCCTCCAAATTCCACCCTTCTCATCGATTCCAATTCCGGCCTCCATTTGTCCTACGCCATTTTCCTCCGTCAAATTCGAAACCTTGCCTCAAATCTCAAATCTTTAACCTCCCTATGCAATGGCCATGTCGCGTTTATCCTCGCACCCACTTCTCTCCAAATCCCTGTGTTGTATTTCGCTCTGTTATCTCTCGGCGTCGTTCTCTCTCCGGCTAATCCAACCAGTTCCGTTTCAGAAATTTCTCATCAGATTCAACTCAGTAAACCGGTCATTGCCTTCGCTACATCCTCAACCGCCTCCAAGCTCCCTACGCTTCGATTCGGCACCGTTATCATCGATTCGCCTCATTTCCTCTCTATGTTGACCGAAACCAACGGATCCGATGGTCTCACCGACATCAAAATCGATCAATCCGACTCGGCGGCGATTCTTTACTCATCAGGTACTACAGGACGAGTGAAAGGCGTGCTACTGAGTCATCGGAACCTTATCGCGGTGAACTCAGGGCCAACTGCTTTTCAATCGGAGATCCACGAAGGAGAAATGAAACCGCATCCAGTTGCTCTGTGTTTGTTGCCTTTATTTCATGTTTTCGGATTCGTAATGTTGGTTCGAGCGATTTCACGGGGAGAAACGTTGGTTTTAATGGAGAGATTCGAATTCGAAGGGATGCTAAGAGCAGTAGAGAAGTTCAGGGTTATCTACATTCCAGTTTCGCCGCCGCTGGTGGTGGCGATGGCGAAATCAGATCTGGTGGCGAAATATGATCTGAGTTCTCTTCAAATTTTGGGATGCGGCGGCGCTCCGCTTGGGAAAGAAGTGATCGATAAATTCCATCAGAAATTGCCCAGCGTTGAAATTGCCCAGGTTTGTTACAAATATTTTCTACTTCTTTTCCTCAATATTTAATTTTTAGGGGTATTACTGACTTTTCAAGACTAACTCAATTATTCGGAATCTAAATAATATTATTGTTTTGACCATAGATGCATAAAGATTATTGCAAAACTTTAGAACTTTAAACTTTTGATTTCATTAAACTGACCATCTTTCATGTTCTCTTGTACTAGAAAATCTTTAGTAAAATTTAGGGTTTAAGATGGTACTATACTAATATTTATCGAAGGTTGAGCTCCAGTTTAAGATTTATTTGAATTTGGTATATAGCTTGTTATAAGAGGAAGTTTGATATGACTTTAGGAAGAATTGATTTTTTAAATTCCAAGTTTTAAAGATTCTTTCTAGATTCATTATGAACTATTACAACTCAAGAGCTAACATTTTTCTTCTCACTTTGGGCTAAATTACATTTTTATCTTCTTTGAAAGGGCCCAAATTTAGTCCTAAATCTTAAAGTTTTTCATTGAGTTTGTAATTAATTGTTATTTGATGCTGGTGAAAGCATTATAACCATTTTCATTCAAGCTATATATCTATCTATACATGGATGAGAATTAATTTTTTAGTAAAATTATGGTTAATTCAATATGGCTTGACATTGTGGTGTTTTCTTGAGTAGGGATACGGCTTGACAGAGAGTACAGCAGGTGCAGCGAGAACTATGGAGCCCGAGGAAATTAGCAACACGAAATCAGTAGGTCGCTTATCTGGAAGTATGGAAGCCAAGATAGTGGATCCTGCATCTGGAGAAGCCTTACTTCCTAACCACAAAGGAGAGCTTTGGCTGCGAGGTCCAACGATCATGAAGGGTGAGCAATGTTCTGTTCTACTTCATCATATTCAATGCTTTGAAACACTCAATATATTTGGACTTGGGTTCACTCTTGTTGAAGATTCTTCATTGAAAAGATAGAAAGATCTCAATATTGCTAACTGGTTTTGAAATTAAACTGCATATTTATCTAATAGGATTCACAAAACCACATTGTGTCCATTTGAACTTGTGACTACCGAATGGCTAATGAAGTGGTGAAGGAGGCGTGTTATATTAGTTATATATGCATACTAGATTTGTAACTATCATGATCTTGGATTTTATTCTCGTTCTATTTGTTAACTCGAGTATAATTCAAGTGATTAATGCACTACATTATCAACCAAACAGATCAGGGGTTCGATAAACAGCTCATTGAGTGTTGTTTGATGCAGGTTACGTTGGAGATGACAAGGCAACTACTGAAACCTTGGATCCAGATGGATGGCTAAAAACTGGCGATCTTTGCTATTTTGATTCAGATGGATTCCTCTACATTGTTGATAGATTAAAAGAATTGATCAAGTACAAGGCTTATCAGGTGACATCTTTACTCTGAGCAATTGCAGATTTCTCAAAAATACGATTAGGAACGTCCCCTTTCATTACTATTTCTATTTTAAACACTTAGAAAGTCAATCCAAACAGTCTCTTAGTCCCTCCCTCTTTAGTAATCAGAAATCCTTTTCTGTTTGGAACAAATTCCAGGTCCCACCAGCAGAATTGGAACATTTGCTTCAATCCAACTCCGAAATCATTGACGCTGCCGTGATACCGTATGCATCTTTCTATCTTGCTTAGTAGTAAACAGTTGAAACTTGTGGCGAATGAAATTTTTTAGACCAAATTTTCTAGATGATGTTTTTATTTCTGTTGTTGAGCAGCTATCCTGATGAAGAAGCTGGAGAGATTCCTATGGCTTATGTTGTTAGAAAGCCCGGAAGTAATATCTCTGAGGCTCAAGTTATTGATTTCATTGCAAAACAGGCAATGCTTCATCTCTCAAGAAACTTCCAAATAGTCCGCTATTTCTCTCGAGTCTTTACCTACATTCTAAAACTGGACCATTTTCATCTTGTAGGTTGCGCCATACAAGAAAATTCGACGAGTCTCTTTTATCGACGCAATCCCTAAATCGCCTGCAGGTAAGATTCTGAGGAGAGAGCTTGCCAAACATGCTCTATCTCATGGTTCTAGTAAGCTGTAAGAAATTAATGTTTCCATGAAAATGTACATATAGTTTGAGTAGCTTAGGTTCATTAATCTCTAAAAATTAAATGGTCACCAAATGAATAAGCATGGCTAAACAGTTATTGTTTTAGTTAATTTAAAGAAGTTTGTTTGATTCTATATGAAACGCTCCTATGAAAATGAATGGATGTCGAGAATTTTTTATGTATGATACATTATAAATATCGATTTTGAATAGACATTCTTTTTATTGTCTTCGAGTCCACTTCCATGGAGGATCAAAGTT

mRNA sequence

TCTTCTAATTTAATTAATAAGCACTTACAAAAAGTGAATTAGGAGTCCATTCATCATTCCATAATCAAAAAATATATTTTTTTTTCTTCAAGTAAAACCGTTAGTTAATATCTAAAAATTACATATAAAAAGAAAAGTTTGCGTTAATTAGTACCAAACATTTTTCTAATAAATTATAAAATAATCTGAATCAGTCAGCATCACTTTCTTCGAGTTCACTTCCATGGCAGCTCGGAACTCTAACTTCAACGCCGCCCCCCATTCCATTGATCTTCGAAGCGGTTTCTGTCCTCTGACGAAGATTTTCCACAGCCTTCGACCACCGCTCTCACTTCCACCATTATCTCAGCCTCTCTCCGTCACCCAACACGCCTTTTCTCTCCTTCAATCATCTCCTCTCCCTCCAAATTCCACCCTTCTCATCGATTCCAATTCCGGCCTCCATTTGTCCTACGCCATTTTCCTCCGTCAAATTCGAAACCTTGCCTCAAATCTCAAATCTTTAACCTCCCTATGCAATGGCCATGTCGCGTTTATCCTCGCACCCACTTCTCTCCAAATCCCTGTGTTGTATTTCGCTCTGTTATCTCTCGGCGTCGTTCTCTCTCCGGCTAATCCAACCAGTTCCGTTTCAGAAATTTCTCATCAGATTCAACTCAGTAAACCGGTCATTGCCTTCGCTACATCCTCAACCGCCTCCAAGCTCCCTACGCTTCGATTCGGCACCGTTATCATCGATTCGCCTCATTTCCTCTCTATGTTGACCGAAACCAACGGATCCGATGGTCTCACCGACATCAAAATCGATCAATCCGACTCGGCGGCGATTCTTTACTCATCAGGTACTACAGGACGAGTGAAAGGCGTGCTACTGAGTCATCGGAACCTTATCGCGGTGAACTCAGGGCCAACTGCTTTTCAATCGGAGATCCACGAAGGAGAAATGAAACCGCATCCAGTTGCTCTGTGTTTGTTGCCTTTATTTCATGTTTTCGGATTCGTAATGTTGGTTCGAGCGATTTCACGGGGAGAAACGTTGGTTTTAATGGAGAGATTCGAATTCGAAGGGATGCTAAGAGCAGTAGAGAAGTTCAGGGTTATCTACATTCCAGTTTCGCCGCCGCTGGTGGTGGCGATGGCGAAATCAGATCTGGTGGCGAAATATGATCTGAGTTCTCTTCAAATTTTGGGATGCGGCGGCGCTCCGCTTGGGAAAGAAGTGATCGATAAATTCCATCAGAAATTGCCCAGCGTTGAAATTGCCCAGGGATACGGCTTGACAGAGAGTACAGCAGGTGCAGCGAGAACTATGGAGCCCGAGGAAATTAGCAACACGAAATCAGTAGGTCGCTTATCTGGAAGTATGGAAGCCAAGATAGTGGATCCTGCATCTGGAGAAGCCTTACTTCCTAACCACAAAGGAGAGCTTTGGCTGCGAGGTCCAACGATCATGAAGGGTTACGTTGGAGATGACAAGGCAACTACTGAAACCTTGGATCCAGATGGATGGCTAAAAACTGGCGATCTTTGCTATTTTGATTCAGATGGATTCCTCTACATTGTTGATAGATTAAAAGAATTGATCAAGTACAAGGCTTATCAGGTCCCACCAGCAGAATTGGAACATTTGCTTCAATCCAACTCCGAAATCATTGACGCTGCCGTGATACCCTATCCTGATGAAGAAGCTGGAGAGATTCCTATGGCTTATGTTGTTAGAAAGCCCGGAAGTAATATCTCTGAGGCTCAAGTTATTGATTTCATTGCAAAACAGGTTGCGCCATACAAGAAAATTCGACGAGTCTCTTTTATCGACGCAATCCCTAAATCGCCTGCAGGTAAGATTCTGAGGAGAGAGCTTGCCAAACATGCTCTATCTCATGGTTCTAGTAAGCTGTAAGAAATTAATGTTTCCATGAAAATGTACATATAGTTTGAGTAGCTTAGGTTCATTAATCTCTAAAAATTAAATGGTCACCAAATGAATAAGCATGGCTAAACAGTTATTGTTTTAGTTAATTTAAAGAAGTTTGTTTGATTCTATATGAAACGCTCCTATGAAAATGAATGGATGTCGAGAATTTTTTATGTATGATACATTATAAATATCGATTTTGAATAGACATTCTTTTTATTGTCTTCGAGTCCACTTCCATGGAGGATCAAAGTT

Coding sequence (CDS)

ATGGCAGCTCGGAACTCTAACTTCAACGCCGCCCCCCATTCCATTGATCTTCGAAGCGGTTTCTGTCCTCTGACGAAGATTTTCCACAGCCTTCGACCACCGCTCTCACTTCCACCATTATCTCAGCCTCTCTCCGTCACCCAACACGCCTTTTCTCTCCTTCAATCATCTCCTCTCCCTCCAAATTCCACCCTTCTCATCGATTCCAATTCCGGCCTCCATTTGTCCTACGCCATTTTCCTCCGTCAAATTCGAAACCTTGCCTCAAATCTCAAATCTTTAACCTCCCTATGCAATGGCCATGTCGCGTTTATCCTCGCACCCACTTCTCTCCAAATCCCTGTGTTGTATTTCGCTCTGTTATCTCTCGGCGTCGTTCTCTCTCCGGCTAATCCAACCAGTTCCGTTTCAGAAATTTCTCATCAGATTCAACTCAGTAAACCGGTCATTGCCTTCGCTACATCCTCAACCGCCTCCAAGCTCCCTACGCTTCGATTCGGCACCGTTATCATCGATTCGCCTCATTTCCTCTCTATGTTGACCGAAACCAACGGATCCGATGGTCTCACCGACATCAAAATCGATCAATCCGACTCGGCGGCGATTCTTTACTCATCAGGTACTACAGGACGAGTGAAAGGCGTGCTACTGAGTCATCGGAACCTTATCGCGGTGAACTCAGGGCCAACTGCTTTTCAATCGGAGATCCACGAAGGAGAAATGAAACCGCATCCAGTTGCTCTGTGTTTGTTGCCTTTATTTCATGTTTTCGGATTCGTAATGTTGGTTCGAGCGATTTCACGGGGAGAAACGTTGGTTTTAATGGAGAGATTCGAATTCGAAGGGATGCTAAGAGCAGTAGAGAAGTTCAGGGTTATCTACATTCCAGTTTCGCCGCCGCTGGTGGTGGCGATGGCGAAATCAGATCTGGTGGCGAAATATGATCTGAGTTCTCTTCAAATTTTGGGATGCGGCGGCGCTCCGCTTGGGAAAGAAGTGATCGATAAATTCCATCAGAAATTGCCCAGCGTTGAAATTGCCCAGGGATACGGCTTGACAGAGAGTACAGCAGGTGCAGCGAGAACTATGGAGCCCGAGGAAATTAGCAACACGAAATCAGTAGGTCGCTTATCTGGAAGTATGGAAGCCAAGATAGTGGATCCTGCATCTGGAGAAGCCTTACTTCCTAACCACAAAGGAGAGCTTTGGCTGCGAGGTCCAACGATCATGAAGGGTTACGTTGGAGATGACAAGGCAACTACTGAAACCTTGGATCCAGATGGATGGCTAAAAACTGGCGATCTTTGCTATTTTGATTCAGATGGATTCCTCTACATTGTTGATAGATTAAAAGAATTGATCAAGTACAAGGCTTATCAGGTCCCACCAGCAGAATTGGAACATTTGCTTCAATCCAACTCCGAAATCATTGACGCTGCCGTGATACCCTATCCTGATGAAGAAGCTGGAGAGATTCCTATGGCTTATGTTGTTAGAAAGCCCGGAAGTAATATCTCTGAGGCTCAAGTTATTGATTTCATTGCAAAACAGGTTGCGCCATACAAGAAAATTCGACGAGTCTCTTTTATCGACGCAATCCCTAAATCGCCTGCAGGTAAGATTCTGAGGAGAGAGCTTGCCAAACATGCTCTATCTCATGGTTCTAGTAAGCTGTAA

Protein sequence

MAARNSNFNAAPHSIDLRSGFCPLTKIFHSLRPPLSLPPLSQPLSVTQHAFSLLQSSPLPPNSTLLIDSNSGLHLSYAIFLRQIRNLASNLKSLTSLCNGHVAFILAPTSLQIPVLYFALLSLGVVLSPANPTSSVSEISHQIQLSKPVIAFATSSTASKLPTLRFGTVIIDSPHFLSMLTETNGSDGLTDIKIDQSDSAAILYSSGTTGRVKGVLLSHRNLIAVNSGPTAFQSEIHEGEMKPHPVALCLLPLFHVFGFVMLVRAISRGETLVLMERFEFEGMLRAVEKFRVIYIPVSPPLVVAMAKSDLVAKYDLSSLQILGCGGAPLGKEVIDKFHQKLPSVEIAQGYGLTESTAGAARTMEPEEISNTKSVGRLSGSMEAKIVDPASGEALLPNHKGELWLRGPTIMKGYVGDDKATTETLDPDGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELEHLLQSNSEIIDAAVIPYPDEEAGEIPMAYVVRKPGSNISEAQVIDFIAKQVAPYKKIRRVSFIDAIPKSPAGKILRRELAKHALSHGSSKL
Homology
BLAST of Cmc12g0334251 vs. NCBI nr
Match: XP_016899600.1 (PREDICTED: 4-coumarate--CoA ligase-like 9 [Cucumis melo])

HSP 1 Score: 1077.4 bits (2785), Expect = 0.0e+00
Identity = 555/558 (99.46%), Postives = 555/558 (99.46%), Query Frame = 0

Query: 1   MAARNSNFNAAPHSIDLRSGFCPLTKIFHSLRPPLSLPPLSQPLSVTQHAFSLLQSSPLP 60
           MAARNSNFNAAPHSIDLRSGFCPLTKIFHSLRPPLSLPPLSQPLSVTQHAFSLLQSS  P
Sbjct: 1   MAARNSNFNAAPHSIDLRSGFCPLTKIFHSLRPPLSLPPLSQPLSVTQHAFSLLQSSSPP 60

Query: 61  PNSTLLIDSNSGLHLSYAIFLRQIRNLASNLKSLTSLCNGHVAFILAPTSLQIPVLYFAL 120
           PNSTLLIDSNSGLHLSYAIFLRQIRNLASNLKSLTSLCNGHVAFILAPTSLQIPVLYFAL
Sbjct: 61  PNSTLLIDSNSGLHLSYAIFLRQIRNLASNLKSLTSLCNGHVAFILAPTSLQIPVLYFAL 120

Query: 121 LSLGVVLSPANPTSSVSEISHQIQLSKPVIAFATSSTASKLPTLRFGTVIIDSPHFLSML 180
           LSLGVVLSPANPTSSVSEISHQIQLSKPVIAFATSSTASKLPTLRFGTVIIDSPHFLSML
Sbjct: 121 LSLGVVLSPANPTSSVSEISHQIQLSKPVIAFATSSTASKLPTLRFGTVIIDSPHFLSML 180

Query: 181 TETNGSDGLTDIKIDQSDSAAILYSSGTTGRVKGVLLSHRNLIAVNSGPTAFQSEIHEGE 240
           TETNGSDGLTDIKIDQSDSAAILYSSGTTGRVKGVLLSHRNLIAVNSGPTAFQSEIHEGE
Sbjct: 181 TETNGSDGLTDIKIDQSDSAAILYSSGTTGRVKGVLLSHRNLIAVNSGPTAFQSEIHEGE 240

Query: 241 MKPHPVALCLLPLFHVFGFVMLVRAISRGETLVLMERFEFEGMLRAVEKFRVIYIPVSPP 300
           MKPHPVALCLLPLFHVFGFVMLVRAISRGETLVLMERFEFEGMLRAVEKFRVIYIPVSPP
Sbjct: 241 MKPHPVALCLLPLFHVFGFVMLVRAISRGETLVLMERFEFEGMLRAVEKFRVIYIPVSPP 300

Query: 301 LVVAMAKSDLVAKYDLSSLQILGCGGAPLGKEVIDKFHQKLPSVEIAQGYGLTESTAGAA 360
           LVVAMAKSDLVAKYDLSSLQILGCGGAPLGKEVIDKFHQKLPSVEIAQGYGLTESTAGAA
Sbjct: 301 LVVAMAKSDLVAKYDLSSLQILGCGGAPLGKEVIDKFHQKLPSVEIAQGYGLTESTAGAA 360

Query: 361 RTMEPEEISNTKSVGRLSGSMEAKIVDPASGEALLPNHKGELWLRGPTIMKGYVGDDKAT 420
           RTMEPEEISNTKSVGRLSGSMEAKIVDPASGEALLPNHKGELWLRGPTIMKGYVGDDKAT
Sbjct: 361 RTMEPEEISNTKSVGRLSGSMEAKIVDPASGEALLPNHKGELWLRGPTIMKGYVGDDKAT 420

Query: 421 TETLDPDGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELEHLLQSNSEIIDAA 480
           TETLDPDGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELEHLLQSN EIIDAA
Sbjct: 421 TETLDPDGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELEHLLQSNPEIIDAA 480

Query: 481 VIPYPDEEAGEIPMAYVVRKPGSNISEAQVIDFIAKQVAPYKKIRRVSFIDAIPKSPAGK 540
           VIPYPDEEAGEIPMAYVVRKPGSNISEAQVIDFIAKQVAPYKKIRRVSFIDAIPKSPAGK
Sbjct: 481 VIPYPDEEAGEIPMAYVVRKPGSNISEAQVIDFIAKQVAPYKKIRRVSFIDAIPKSPAGK 540

Query: 541 ILRRELAKHALSHGSSKL 559
           ILRRELAKHALSHGSSKL
Sbjct: 541 ILRRELAKHALSHGSSKL 558

BLAST of Cmc12g0334251 vs. NCBI nr
Match: KAA0055316.1 (4-coumarate-CoA ligase [Cucumis melo var. makuwa])

HSP 1 Score: 1076.6 bits (2783), Expect = 0.0e+00
Identity = 558/569 (98.07%), Postives = 558/569 (98.07%), Query Frame = 0

Query: 1   MAARNSNFNAAPHSIDLRSGFCPLTKIFHSLRPPLSLPPLSQPLSVTQHAFSLLQSSPLP 60
           MAARNSNFNAAPHSIDLRSGFCPLTKIFHSLRPPLSLPPLSQPLSVTQHAFSLLQSSPLP
Sbjct: 1   MAARNSNFNAAPHSIDLRSGFCPLTKIFHSLRPPLSLPPLSQPLSVTQHAFSLLQSSPLP 60

Query: 61  PNSTLLIDSNSGLHLSYAIFLRQIRNLASNLKSLTSLCNGHVAFILAPTSLQIPVLYFAL 120
           PNSTLLIDSNSGLHLSYAIFLRQIRNLASNLKSLTSLCNGHVAFILAPTSLQIPVLYFAL
Sbjct: 61  PNSTLLIDSNSGLHLSYAIFLRQIRNLASNLKSLTSLCNGHVAFILAPTSLQIPVLYFAL 120

Query: 121 LSLGVVLSPANPTSSVSEISHQIQLSKPVIAFATSSTASKLPTLRFGTVIIDSPHFLSML 180
           LSLGVVLSPANPTSSVSEISHQIQLSKPVIAFATSSTASKLPTLRFGTVIIDSPHFLSML
Sbjct: 121 LSLGVVLSPANPTSSVSEISHQIQLSKPVIAFATSSTASKLPTLRFGTVIIDSPHFLSML 180

Query: 181 TETNGSDGLTDIKIDQSDSAAILYSSGTTGRVKGVLLSHRNLIAVNSGPTAFQSEIHEGE 240
           TETNGSDGLTDIKIDQSDSAAILYSSGTTGRVKGVLLSHRNLIAVNSGPTAFQSEIHEGE
Sbjct: 181 TETNGSDGLTDIKIDQSDSAAILYSSGTTGRVKGVLLSHRNLIAVNSGPTAFQSEIHEGE 240

Query: 241 MKPHPVALCLLPLFHVFGFVMLVRAISRGETLVLMERFEFEGMLRAVEKFRVIYIPVSPP 300
           MKPHPVALCLLPLFHVFGFVMLVRAISRGETLVLMERFEFEGMLRAVEKFRVIYIPVSPP
Sbjct: 241 MKPHPVALCLLPLFHVFGFVMLVRAISRGETLVLMERFEFEGMLRAVEKFRVIYIPVSPP 300

Query: 301 LVVAMAKSDLVAKYDLSSLQILGCGGAPLGKEVIDKFHQKLPSVEIAQGYGLTESTAGAA 360
           LVVAMAKSDLVAKYDLSSLQILGCGGAPLGKEVIDKFHQKLPSVEIAQGYGLTESTAGAA
Sbjct: 301 LVVAMAKSDLVAKYDLSSLQILGCGGAPLGKEVIDKFHQKLPSVEIAQGYGLTESTAGAA 360

Query: 361 RTMEPEEISNTKSVGRLSGSMEAKIVDPASGEALLPNHKGELWLRGPTIMKGYVGDDKAT 420
           RTMEPEEISNTKSVGRLSGSMEAKIVDPASGEALLPNHKGELWLRGPTIMKGYVGDDKAT
Sbjct: 361 RTMEPEEISNTKSVGRLSGSMEAKIVDPASGEALLPNHKGELWLRGPTIMKGYVGDDKAT 420

Query: 421 TETLDPDGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELEHLLQSNSEIIDAA 480
           TETLDPDGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELEHLLQSNSEIIDAA
Sbjct: 421 TETLDPDGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELEHLLQSNSEIIDAA 480

Query: 481 VIPYPDEEAGEIPMAYVVRKPGSNISEAQVIDFIAKQ-----------VAPYKKIRRVSF 540
           VIPYPDEEAGEIPMAYVVRKPGSNISEAQVIDFIAKQ           VAPYKKIRRVSF
Sbjct: 481 VIPYPDEEAGEIPMAYVVRKPGSNISEAQVIDFIAKQAMLHLSRNFQIVAPYKKIRRVSF 540

Query: 541 IDAIPKSPAGKILRRELAKHALSHGSSKL 559
           IDAIPKSPAGKILRRELAKHALSHGSSKL
Sbjct: 541 IDAIPKSPAGKILRRELAKHALSHGSSKL 569

BLAST of Cmc12g0334251 vs. NCBI nr
Match: TYJ99241.1 (4-coumarate-CoA ligase [Cucumis melo var. makuwa])

HSP 1 Score: 1068.9 bits (2763), Expect = 1.5e-308
Identity = 555/569 (97.54%), Postives = 555/569 (97.54%), Query Frame = 0

Query: 1   MAARNSNFNAAPHSIDLRSGFCPLTKIFHSLRPPLSLPPLSQPLSVTQHAFSLLQSSPLP 60
           MAARNSNFNAAPHSIDLRSGFCPLTKIFHSLRPPLSLPPLSQPLSVTQHAFSLLQSS  P
Sbjct: 1   MAARNSNFNAAPHSIDLRSGFCPLTKIFHSLRPPLSLPPLSQPLSVTQHAFSLLQSSSPP 60

Query: 61  PNSTLLIDSNSGLHLSYAIFLRQIRNLASNLKSLTSLCNGHVAFILAPTSLQIPVLYFAL 120
           PNSTLLIDSNSGLHLSYAIFLRQIRNLASNLKSLTSLCNGHVAFILAPTSLQIPVLYFAL
Sbjct: 61  PNSTLLIDSNSGLHLSYAIFLRQIRNLASNLKSLTSLCNGHVAFILAPTSLQIPVLYFAL 120

Query: 121 LSLGVVLSPANPTSSVSEISHQIQLSKPVIAFATSSTASKLPTLRFGTVIIDSPHFLSML 180
           LSLGVVLSPANPTSSVSEISHQIQLSKPVIAFATSSTASKLPTLRFGTVIIDSPHFLSML
Sbjct: 121 LSLGVVLSPANPTSSVSEISHQIQLSKPVIAFATSSTASKLPTLRFGTVIIDSPHFLSML 180

Query: 181 TETNGSDGLTDIKIDQSDSAAILYSSGTTGRVKGVLLSHRNLIAVNSGPTAFQSEIHEGE 240
           TETNGSDGLTDIKIDQSDSAAILYSSGTTGRVKGVLLSHRNLIAVNSGPTAFQSEIHEGE
Sbjct: 181 TETNGSDGLTDIKIDQSDSAAILYSSGTTGRVKGVLLSHRNLIAVNSGPTAFQSEIHEGE 240

Query: 241 MKPHPVALCLLPLFHVFGFVMLVRAISRGETLVLMERFEFEGMLRAVEKFRVIYIPVSPP 300
           MKPHPVALCLLPLFHVFGFVMLVRAISRGETLVLMERFEFEGMLRAVEKFRVIYIPVSPP
Sbjct: 241 MKPHPVALCLLPLFHVFGFVMLVRAISRGETLVLMERFEFEGMLRAVEKFRVIYIPVSPP 300

Query: 301 LVVAMAKSDLVAKYDLSSLQILGCGGAPLGKEVIDKFHQKLPSVEIAQGYGLTESTAGAA 360
           LVVAMAKSDLVAKYDLSSLQILGCGGAPLGKEVIDKFHQKLPSVEIAQGYGLTESTAGAA
Sbjct: 301 LVVAMAKSDLVAKYDLSSLQILGCGGAPLGKEVIDKFHQKLPSVEIAQGYGLTESTAGAA 360

Query: 361 RTMEPEEISNTKSVGRLSGSMEAKIVDPASGEALLPNHKGELWLRGPTIMKGYVGDDKAT 420
           RTMEPEEISNTKSVGRLSGSMEAKIVDPASGEALLPNHKGELWLRGPTIMKGYVGDDKAT
Sbjct: 361 RTMEPEEISNTKSVGRLSGSMEAKIVDPASGEALLPNHKGELWLRGPTIMKGYVGDDKAT 420

Query: 421 TETLDPDGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELEHLLQSNSEIIDAA 480
           TETLDPDGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELEHLLQSN EIIDAA
Sbjct: 421 TETLDPDGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELEHLLQSNPEIIDAA 480

Query: 481 VIPYPDEEAGEIPMAYVVRKPGSNISEAQVIDFIAKQ-----------VAPYKKIRRVSF 540
           VIPYPDEEAGEIPMAYVVRKPGSNISEAQVIDFIAKQ           VAPYKKIRRVSF
Sbjct: 481 VIPYPDEEAGEIPMAYVVRKPGSNISEAQVIDFIAKQAMLHLSRNFQIVAPYKKIRRVSF 540

Query: 541 IDAIPKSPAGKILRRELAKHALSHGSSKL 559
           IDAIPKSPAGKILRRELAKHALSHGSSKL
Sbjct: 541 IDAIPKSPAGKILRRELAKHALSHGSSKL 569

BLAST of Cmc12g0334251 vs. NCBI nr
Match: ADN33954.1 (4-coumarate-CoA ligase [Cucumis melo subsp. melo])

HSP 1 Score: 1068.9 bits (2763), Expect = 1.5e-308
Identity = 555/569 (97.54%), Postives = 555/569 (97.54%), Query Frame = 0

Query: 1    MAARNSNFNAAPHSIDLRSGFCPLTKIFHSLRPPLSLPPLSQPLSVTQHAFSLLQSSPLP 60
            MAARNSNFNAAPHSIDLRSGFCPLTKIFHSLRPPLSLPPLSQPLSVTQHAFSLLQSS  P
Sbjct: 487  MAARNSNFNAAPHSIDLRSGFCPLTKIFHSLRPPLSLPPLSQPLSVTQHAFSLLQSSSPP 546

Query: 61   PNSTLLIDSNSGLHLSYAIFLRQIRNLASNLKSLTSLCNGHVAFILAPTSLQIPVLYFAL 120
            PNSTLLIDSNSGLHLSYAIFLRQIRNLASNLKSLTSLCNGHVAFILAPTSLQIPVLYFAL
Sbjct: 547  PNSTLLIDSNSGLHLSYAIFLRQIRNLASNLKSLTSLCNGHVAFILAPTSLQIPVLYFAL 606

Query: 121  LSLGVVLSPANPTSSVSEISHQIQLSKPVIAFATSSTASKLPTLRFGTVIIDSPHFLSML 180
            LSLGVVLSPANPTSSVSEISHQIQLSKPVIAFATSSTASKLPTLRFGTVIIDSPHFLSML
Sbjct: 607  LSLGVVLSPANPTSSVSEISHQIQLSKPVIAFATSSTASKLPTLRFGTVIIDSPHFLSML 666

Query: 181  TETNGSDGLTDIKIDQSDSAAILYSSGTTGRVKGVLLSHRNLIAVNSGPTAFQSEIHEGE 240
            TETNGSDGLTDIKIDQSDSAAILYSSGTTGRVKGVLLSHRNLIAVNSGPTAFQSEIHEGE
Sbjct: 667  TETNGSDGLTDIKIDQSDSAAILYSSGTTGRVKGVLLSHRNLIAVNSGPTAFQSEIHEGE 726

Query: 241  MKPHPVALCLLPLFHVFGFVMLVRAISRGETLVLMERFEFEGMLRAVEKFRVIYIPVSPP 300
            MKPHPVALCLLPLFHVFGFVMLVRAISRGETLVLMERFEFEGMLRAVEKFRVIYIPVSPP
Sbjct: 727  MKPHPVALCLLPLFHVFGFVMLVRAISRGETLVLMERFEFEGMLRAVEKFRVIYIPVSPP 786

Query: 301  LVVAMAKSDLVAKYDLSSLQILGCGGAPLGKEVIDKFHQKLPSVEIAQGYGLTESTAGAA 360
            LVVAMAKSDLVAKYDLSSLQILGCGGAPLGKEVIDKFHQKLPSVEIAQGYGLTESTAGAA
Sbjct: 787  LVVAMAKSDLVAKYDLSSLQILGCGGAPLGKEVIDKFHQKLPSVEIAQGYGLTESTAGAA 846

Query: 361  RTMEPEEISNTKSVGRLSGSMEAKIVDPASGEALLPNHKGELWLRGPTIMKGYVGDDKAT 420
            RTMEPEEISNTKSVGRLSGSMEAKIVDPASGEALLPNHKGELWLRGPTIMKGYVGDDKAT
Sbjct: 847  RTMEPEEISNTKSVGRLSGSMEAKIVDPASGEALLPNHKGELWLRGPTIMKGYVGDDKAT 906

Query: 421  TETLDPDGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELEHLLQSNSEIIDAA 480
            TETLDPDGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELEHLLQSN EIIDAA
Sbjct: 907  TETLDPDGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELEHLLQSNPEIIDAA 966

Query: 481  VIPYPDEEAGEIPMAYVVRKPGSNISEAQVIDFIAKQ-----------VAPYKKIRRVSF 540
            VIPYPDEEAGEIPMAYVVRKPGSNISEAQVIDFIAKQ           VAPYKKIRRVSF
Sbjct: 967  VIPYPDEEAGEIPMAYVVRKPGSNISEAQVIDFIAKQAMLHLSRNFQIVAPYKKIRRVSF 1026

Query: 541  IDAIPKSPAGKILRRELAKHALSHGSSKL 559
            IDAIPKSPAGKILRRELAKHALSHGSSKL
Sbjct: 1027 IDAIPKSPAGKILRRELAKHALSHGSSKL 1055

BLAST of Cmc12g0334251 vs. NCBI nr
Match: XP_011652003.1 (4-coumarate--CoA ligase-like 9 [Cucumis sativus] >KGN64389.1 hypothetical protein Csa_013692 [Cucumis sativus])

HSP 1 Score: 1011.9 bits (2615), Expect = 2.1e-291
Identity = 515/558 (92.29%), Postives = 534/558 (95.70%), Query Frame = 0

Query: 1   MAARNSNFNAAPHSIDLRSGFCPLTKIFHSLRPPLSLPPLSQPLSVTQHAFSLLQSSPLP 60
           MA RNSNF  +PHSIDLRSGFCP TKIFHSLRPPLSLPPLS PLSV QHAFSLLQSSP P
Sbjct: 1   MADRNSNFTTSPHSIDLRSGFCPQTKIFHSLRPPLSLPPLSHPLSVIQHAFSLLQSSPPP 60

Query: 61  PNSTLLIDSNSGLHLSYAIFLRQIRNLASNLKSLTSLCNGHVAFILAPTSLQIPVLYFAL 120
           PNSTLLID NSGLHLSY +FLRQIRNLASNLKSLTSLCNG VAFILAPTSLQIPVLYFAL
Sbjct: 61  PNSTLLIDFNSGLHLSYTVFLRQIRNLASNLKSLTSLCNGQVAFILAPTSLQIPVLYFAL 120

Query: 121 LSLGVVLSPANPTSSVSEISHQIQLSKPVIAFATSSTASKLPTLRFGTVIIDSPHFLSML 180
           LSLGVVLSPANPTSSVSEISHQIQLSKPVIAFATSSTASKLP LRFGTV+IDSPHF SML
Sbjct: 121 LSLGVVLSPANPTSSVSEISHQIQLSKPVIAFATSSTASKLPKLRFGTVLIDSPHFHSML 180

Query: 181 TETNGSDGLTDIKIDQSDSAAILYSSGTTGRVKGVLLSHRNLIAVNSGPTAFQSEIHEGE 240
           TETNGSDG+ +IKIDQSDSAAILYSSGTTGRVKGVLLSHRNLIAVN+GP + Q EI EGE
Sbjct: 181 TETNGSDGIPNIKIDQSDSAAILYSSGTTGRVKGVLLSHRNLIAVNAGPNSLQLEIQEGE 240

Query: 241 MKPHPVALCLLPLFHVFGFVMLVRAISRGETLVLMERFEFEGMLRAVEKFRVIYIPVSPP 300
           M+PHPV LC LPLFH+FGFVMLVRA+SRG+TLVLMERFEFEGMLRAVEK+RV YIPVSPP
Sbjct: 241 MEPHPVVLCPLPLFHIFGFVMLVRAVSRGDTLVLMERFEFEGMLRAVEKYRVKYIPVSPP 300

Query: 301 LVVAMAKSDLVAKYDLSSLQILGCGGAPLGKEVIDKFHQKLPSVEIAQGYGLTESTAGAA 360
           LVVAMAKS+LV KYDLSSLQ+LGCGGAPLGKEVIDKFHQKLP VEI QGYGLTESTAGA+
Sbjct: 301 LVVAMAKSELVGKYDLSSLQMLGCGGAPLGKEVIDKFHQKLPGVEIVQGYGLTESTAGAS 360

Query: 361 RTMEPEEISNTKSVGRLSGSMEAKIVDPASGEALLPNHKGELWLRGPTIMKGYVGDDKAT 420
           RTM+PEEISNTKSVGRLSG+MEAKIVDPASG+ALLPNHKGELWLRGPTIMKGYVGDDKAT
Sbjct: 361 RTMDPEEISNTKSVGRLSGNMEAKIVDPASGDALLPNHKGELWLRGPTIMKGYVGDDKAT 420

Query: 421 TETLDPDGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELEHLLQSNSEIIDAA 480
           TETLDPDGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELEHLLQSN EIIDAA
Sbjct: 421 TETLDPDGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELEHLLQSNPEIIDAA 480

Query: 481 VIPYPDEEAGEIPMAYVVRKPGSNISEAQVIDFIAKQVAPYKKIRRVSFIDAIPKSPAGK 540
           VIPYPDEEAGEIPMAYVVRKPGSN+SEAQVIDFIAKQVAPYKKIRRVSFIDAIPKSPAGK
Sbjct: 481 VIPYPDEEAGEIPMAYVVRKPGSNMSEAQVIDFIAKQVAPYKKIRRVSFIDAIPKSPAGK 540

Query: 541 ILRRELAKHALSHGSSKL 559
           ILRRELAKHALSHGSSKL
Sbjct: 541 ILRRELAKHALSHGSSKL 558

BLAST of Cmc12g0334251 vs. ExPASy Swiss-Prot
Match: Q84P23 (4-coumarate--CoA ligase-like 9 OS=Arabidopsis thaliana OX=3702 GN=4CLL9 PE=1 SV=2)

HSP 1 Score: 644.4 bits (1661), Expect = 1.2e-183
Identity = 340/555 (61.26%), Postives = 429/555 (77.30%), Query Frame = 0

Query: 15  IDLRSGFCPLTKIFHSLRPPLSLPPLSQPLSVTQHAFSLLQSSPLPPNS-------TLLI 74
           ID  SGF   T I+HSLRP LSLPP+ QPLS  + A SLL  S  P  +       T L+
Sbjct: 11  IDRSSGFDQRTGIYHSLRPSLSLPPIDQPLSAAEFALSLLLKSSPPATAGKNIEALTYLV 70

Query: 75  DSNSGLHLSYAIFLRQIRNLASNLKS-LTSLCNGHVAFILAPTSLQIPVLYFALLSLGVV 134
           +S+SG +L+Y   LR++R+LA +L+    SL + +VAFIL+P+SL IPVLY AL+S+GVV
Sbjct: 71  NSSSGDNLTYGELLRRVRSLAVSLRERFPSLASRNVAFILSPSSLDIPVLYLALMSIGVV 130

Query: 135 LSPANPTSSVSEISHQIQLSKPVIAFATSSTASKL--PTLRFGTVIIDSPHFLSMLTETN 194
           +SPANP  S SE+SHQ+++S+PVIAFATS T  KL   +L  GTV++DS  FLS L  ++
Sbjct: 131 VSPANPIGSESEVSHQVEVSEPVIAFATSQTVKKLQSSSLPLGTVLMDSTEFLSWLNRSD 190

Query: 195 GSD-GLTDIKIDQSDSAAILYSSGTTGRVKGVLLSHRNLIAVNSGPTAFQSEIHEGEMKP 254
            S      ++++QSD AAIL+SSGTTGRVKGVLL+HRNLIA  S   + Q  + +  +  
Sbjct: 191 SSSVNPFQVQVNQSDPAAILFSSGTTGRVKGVLLTHRNLIA--STAVSHQRTLQD-PVNY 250

Query: 255 HPVALCLLPLFHVFGFVMLVRAISRGETLVLMERFEFEGMLRAVEKFRVIYIPVSPPLVV 314
             V L  LPLFHVFGF+M++RAIS GETLVL+ RFE E M +AVEK++V  +PVSPPL+V
Sbjct: 251 DRVGLFSLPLFHVFGFMMMIRAISLGETLVLLGRFELEAMFKAVEKYKVTGMPVSPPLIV 310

Query: 315 AMAKSDLVAKYDLSSLQILGCGGAPLGKEVIDKFHQKLPSVEIAQGYGLTESTAGAARTM 374
           A+ KS+L  KYDL SL+ LGCGGAPLGK++ ++F QK P V+I QGYGLTES+  AA T 
Sbjct: 311 ALVKSELTKKYDLRSLRSLGCGGAPLGKDIAERFKQKFPDVDIVQGYGLTESSGPAASTF 370

Query: 375 EPEEISNTKSVGRLSGSMEAKIVDPASGEALLPNHKGELWLRGPTIMKGYVGDDKATTET 434
            PEE+    SVGR+S +MEAKIVDP++GE+L P   GELWLRGP IMKGYVG++KA+ ET
Sbjct: 371 GPEEMVKYGSVGRISENMEAKIVDPSTGESLPPGKTGELWLRGPVIMKGYVGNEKASAET 430

Query: 435 LDPDGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELEHLLQSNSEIIDAAVIP 494
           +D +GWLKTGDLCYFDS+ FLYIVDRLKELIKYKAYQVPP ELE +L SN ++IDAAV+P
Sbjct: 431 VDKEGWLKTGDLCYFDSEDFLYIVDRLKELIKYKAYQVPPVELEQILHSNPDVIDAAVVP 490

Query: 495 YPDEEAGEIPMAYVVRKPGSNISEAQVIDFIAKQVAPYKKIRRVSFIDAIPKSPAGKILR 554
           +PDE+AGEIPMA++VRKPGSN++EAQ+IDF+AKQV PYKK+RRV+FI+AIPK+PAGKILR
Sbjct: 491 FPDEDAGEIPMAFIVRKPGSNLNEAQIIDFVAKQVTPYKKVRRVAFINAIPKNPAGKILR 550

Query: 555 RELAKHALSHGSSKL 559
           REL K A+   +SKL
Sbjct: 551 RELTKIAVDGNASKL 562

BLAST of Cmc12g0334251 vs. ExPASy Swiss-Prot
Match: Q7F1X5 (4-coumarate--CoA ligase-like 5 OS=Oryza sativa subsp. japonica OX=39947 GN=4CLL5 PE=2 SV=1)

HSP 1 Score: 555.4 bits (1430), Expect = 7.1e-157
Identity = 300/555 (54.05%), Postives = 389/555 (70.09%), Query Frame = 0

Query: 12  PHSIDLRSGFCPLTKIFHSLRPPLSLPPLSQPLSVTQHAFSLLQSSPLPPNSTLLIDSNS 71
           P  ID RSGFC  T+IFHS R P  LPP S P++   +AFSLL SS L P    L+D+ +
Sbjct: 12  PAGIDSRSGFCAATRIFHSTRAPGDLPPESLPMTAAAYAFSLLSSSTL-PGRPALVDAAT 71

Query: 72  GLHLSYAIFLRQIRNLASNLKSLTSLCNGHVAFILAPTSLQIPVLYFALLSLGVVLSPAN 131
           G+ +SY  FL  +R+LA  L     L  G VA ++AP+ L++PVL FAL+S+G V+SPAN
Sbjct: 72  GIAISYPSFLAAVRSLAGGLWCSLGLRPGDVALVVAPSRLEVPVLDFALMSIGAVVSPAN 131

Query: 132 PTSSVSEISHQIQLSKPVIAFATSSTASKLPTLRFGTVIIDSPHFLSMLTETNGSDGLTD 191
           P S+  E +HQ+ LS+PV+AFA    A+KLP      V+I S  +   L  ++G      
Sbjct: 132 PVSTPEEYAHQVALSRPVVAFAAPEVAAKLPE-HVRCVVIGSDEY-GRLAASDGRRAAAP 191

Query: 192 --IKIDQSDSAAILYSSGTTGRVKGVLLSHRNLIAVNSGPTAFQSEI-----HEGEMKPH 251
             + + QSD+AA+LYSSGTTGRVK V ++HRNLIA+ S     + ++       GE  P 
Sbjct: 192 AAVAVKQSDTAAVLYSSGTTGRVKAVAITHRNLIALMSLHADNREKVAREAAEAGEEPPP 251

Query: 252 P-VALCLLPLFHVFGFVMLVRAISRGETLVLMERFEFEGMLRAVEKFRVIYIPVSPPLVV 311
           P V L  +PLFHVFGF+M++R++S GET VLMERF+F   LRA+E++RV  +P +PP++V
Sbjct: 252 PAVTLLPIPLFHVFGFMMVLRSVSMGETSVLMERFDFIAALRAIERYRVTLLPAAPPVLV 311

Query: 312 AMAKSDLVAKYDLSSLQILGCGGAPLGKEVIDKFHQKLPSVEIAQGYGLTESTAGAARTM 371
           AM K +   + DLSSL ++G GGAPLG+EV ++F    P+VE+ QGYGLTES+   A T+
Sbjct: 312 AMVKYEEARRRDLSSLLVIGIGGAPLGREVAEQFASVFPNVELVQGYGLTESSGAVAATV 371

Query: 372 EPEEISNTKSVGRLSGSMEAKIVDPASGEALLPNHKGELWLRGPTIMKGYVGDDKATTET 431
            PEE     SVG+L   ++AKIVDP++                     GYVGDD+AT  T
Sbjct: 372 GPEESKAYGSVGKLGSHLQAKIVDPST---------------------GYVGDDEATAAT 431

Query: 432 LDPDGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELEHLLQSNSEIIDAAVIP 491
           +D +GWLKTGDLCYF+ DGFLYIVDRLKELIKYK YQVPPAELEH+LQS+  I DAAVIP
Sbjct: 432 VDSEGWLKTGDLCYFNEDGFLYIVDRLKELIKYKGYQVPPAELEHILQSHPGIADAAVIP 491

Query: 492 YPDEEAGEIPMAYVVRKPGSNISEAQVIDFIAKQVAPYKKIRRVSFIDAIPKSPAGKILR 551
           YPDEEAGE+PMA++VR+PGSNI++ QV+D++AKQVAPYKK+RRV+F+ AIPKSPAGKILR
Sbjct: 492 YPDEEAGELPMAFIVRQPGSNITKEQVMDYVAKQVAPYKKVRRVAFVTAIPKSPAGKILR 542

Query: 552 RELAKHALSHGSSKL 559
           REL + ALS G+SKL
Sbjct: 552 RELVQQALSMGASKL 542

BLAST of Cmc12g0334251 vs. ExPASy Swiss-Prot
Match: Q8RU95 (4-coumarate--CoA ligase-like 6 OS=Oryza sativa subsp. japonica OX=39947 GN=4CLL6 PE=2 SV=2)

HSP 1 Score: 541.2 bits (1393), Expect = 1.4e-152
Identity = 293/557 (52.60%), Postives = 378/557 (67.86%), Query Frame = 0

Query: 18  RSGFCPLTKIFHSLRPPLSLPPLSQPLSVTQHAFSLLQSSP---LPPNSTLLIDSNSGLH 77
           RSGFC  T+ FHSLR    LPP   PL+V  +AFSLL S+P   +      L+D+ +G+ 
Sbjct: 41  RSGFCAATRTFHSLRSVGPLPPEELPLTVAAYAFSLLSSAPPLVVAGRGPALVDAATGIA 100

Query: 78  LSYAIFLRQIRNLASNLKSLTSLCNGHVAFILAPTSLQIPVLYFALLSLGVVLSPANPTS 137
           +SY  F+ ++R LA  L     L  G VA +++P+ L + VLYFAL+S+GVV+SPANP S
Sbjct: 101 VSYPAFVARVRFLAGGLWCSLGLRPGDVALVVSPSCLDVAVLYFALMSIGVVVSPANPAS 160

Query: 138 SVSEISHQIQLSKPVIAFATSSTASKLPTLRFGTVIIDSPHF--LSMLTETNGSDGLTDI 197
           +  E +HQ++LS+P IAF     A++LP      V+I S  F  L+  +   G      +
Sbjct: 161 TADEYAHQVRLSRPAIAFVAPEVAARLPR-HVSRVVIGSEVFDRLASASAAGGWAAPPAV 220

Query: 198 KIDQSDSAAILYSSGTTGRVKGVLLSHRNLIAVNSGPTAFQSEI------HEGEMKPHP- 257
            + Q  +AA+LYSSGTTGRVK V ++HRNLIA  S   A +  +        G+ KP P 
Sbjct: 221 AMKQPSTAALLYSSGTTGRVKAVAITHRNLIAQISAYNAIRETVAREAATDAGKGKPPPP 280

Query: 258 -------VALCLLPLFHVFGFVMLVRAISRGETLVLMERFEFEGMLRAVEKFRVIYIPVS 317
                  V L  LPLFHV GF +L R IS GET V+M RF+     RAVE++RV  +  +
Sbjct: 281 SPSPPAAVTLFPLPLFHVMGFGLLTRTISSGETAVVMRRFDLAAAARAVERYRVTKLSAA 340

Query: 318 PPLVVAMAKSDLVAKYDLSSLQILGCGGAPLGKEVIDKFHQKLPSVEIAQGYGLTESTAG 377
           PP+VVA+ KSD   + DLSSL  +  GGAPLG+EV  +F    PSV+I Q YGLTEST  
Sbjct: 341 PPVVVALTKSDEARRRDLSSLVAIVVGGAPLGREVSQRFATVFPSVQIVQSYGLTESTGP 400

Query: 378 AARTMEPEEISNTKSVGRLSGSMEAKIVDPASGEALLPNHKGELWLRGPTIMKGYVGDDK 437
            A    PEE +   SVGRL+  ++AKIVD A+GE L P  +GELW+RGP +MKGYVGD +
Sbjct: 401 VATMAGPEESAAYGSVGRLAPRVQAKIVDTATGEVLGPGRRGELWIRGPVVMKGYVGDPE 460

Query: 438 ATTETLDPDGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELEHLLQSNSEIID 497
           AT  T+ PDGWLKTGDLCYF+ DG+LY+VDRLKELIKYK YQVPPAELEH+LQS  EI D
Sbjct: 461 ATAATITPDGWLKTGDLCYFNEDGYLYVVDRLKELIKYKGYQVPPAELEHILQSRPEIAD 520

Query: 498 AAVIPYPDEEAGEIPMAYVVRKPGSNISEAQVIDFIAKQVAPYKKIRRVSFIDAIPKSPA 556
           AAV+PYPDEEAG++PMA+VVR+PG+ ++E QV++ +AK VAPYKK+RRV+F++AIPKSPA
Sbjct: 521 AAVVPYPDEEAGQLPMAFVVRQPGAYLTEQQVMNCVAKHVAPYKKVRRVAFVNAIPKSPA 580

BLAST of Cmc12g0334251 vs. ExPASy Swiss-Prot
Match: Q69RG7 (4-coumarate--CoA ligase-like 7 OS=Oryza sativa subsp. japonica OX=39947 GN=4CLL7 PE=2 SV=1)

HSP 1 Score: 490.7 bits (1262), Expect = 2.1e-137
Identity = 271/562 (48.22%), Postives = 373/562 (66.37%), Query Frame = 0

Query: 1   MAARNSNFNAAPHSIDLRSGFCPLTKIFHSLRPPLSLPPLSQPLSVTQHAFSLL------ 60
           MAA  S  NA   S   R+G+C  TK F SLRPP+ LPP   PLS  + AFSLL      
Sbjct: 1   MAAPTSMANADGES--TRTGYCAATKSFRSLRPPVPLPPPDVPLSFPEFAFSLLPRSSSS 60

Query: 61  QSSPLPPNSTLLIDSNSGLHLSYAIFLRQIRNLASNLKSLTSLCNGHVAFILAPTSLQIP 120
            SS L P +  L+D+ +G  +S+  FL ++R LA  L+S   L  G VAF+LAP  L +P
Sbjct: 61  SSSSLLPANPALVDAATGEAVSFQAFLSRVRALAGALRSRVGLRGGDVAFVLAPAGLDVP 120

Query: 121 VLYFALLSLGVVLSPANPTSSVSEISHQIQLSKPVIAFATSSTASKLPTLRFGTVIIDSP 180
           VLYFALLS+G V+SPANP  + +E+S  + LS   +AFA SSTA+KLP      V++DSP
Sbjct: 121 VLYFALLSIGAVVSPANPALTPAEVSRLVSLSGASVAFAVSSTATKLPAGLTTVVLLDSP 180

Query: 181 HFLSMLT---ETNGSDGLTDIKIDQSDSAAILYSSGTTGRVKGVLLSHRNLIAVNSGPTA 240
           HF S+L    +  G + L  + + QS++AAI YSSGTTGRVK   L HR+ IA+ +G  A
Sbjct: 181 HFRSLLMDCGQAQGQEPLPVVVVRQSETAAIQYSSGTTGRVKAAALPHRSFIAMVAGFHA 240

Query: 241 FQSEIHEGEMKPHPVALCLLPLFHVFGFVMLVRAISRGETLVL----MERFEFEGMLRAV 300
            +++  E         L   P+FH  GF+ +++ ++ G T V+    + R    G++ A 
Sbjct: 241 LRAKAREVR------TLLGAPMFHSMGFLFVLQGVALGATTVVVTDAVARAGIRGLVEAA 300

Query: 301 EKFRVIYIPVSPPLVVAMAKSDLVAKYDLSSLQILGCGGAPLGKEVIDKFHQKLPSVEIA 360
           E++ V+ +  SPP+V+ M K     +  L +L+ + CGGAPL    I++F ++ P V++ 
Sbjct: 301 ERWAVMDMTASPPVVLGMTKQ----RCRLPALERITCGGAPLPAPAIERFRRRFPHVDLC 360

Query: 361 QGYGLTESTAGAARTMEPEEISNTKSVGRLSGSMEAKIVDPASGEALLPNHKGELWLRGP 420
            GYG TE+  G +R +  EE ++  S GR++ ++E KIVD  +G+ L    +GELW+RGP
Sbjct: 361 MGYGSTEA-GGISRMISQEECNHIGSAGRVTENVEVKIVDHVTGKPLPAGQQGELWVRGP 420

Query: 421 TIMKGYVGDDKATTETLDPDGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELE 480
            +M GYVGD++A   T + +GWLKTGDLCY D DGFL++VDRLKELIKYKAYQVPPAELE
Sbjct: 421 AVMTGYVGDNEANATTFNSEGWLKTGDLCYIDQDGFLFVVDRLKELIKYKAYQVPPAELE 480

Query: 481 HLLQSNSEIIDAAVIPYPDEEAGEIPMAYVVRKPGSNISEAQVIDFIAKQVAPYKKIRRV 540
            +L S  +I+DAAV+PYP EEAG+IP+A VV++PGS ++EA+V+  +AKQVAPYKKIR+V
Sbjct: 481 LVLHSLPQIVDAAVMPYPHEEAGQIPVALVVKQPGSKLTEAEVMYNVAKQVAPYKKIRKV 540

Query: 541 SFIDAIPKSPAGKILRRELAKH 550
            F+D+IPKSP+GKILRREL  H
Sbjct: 541 LFVDSIPKSPSGKILRRELVNH 549

BLAST of Cmc12g0334251 vs. ExPASy Swiss-Prot
Match: M4IQR7 (Probable CoA ligase CCL5 OS=Humulus lupulus OX=3486 GN=CCL5 PE=2 SV=1)

HSP 1 Score: 460.3 bits (1183), Expect = 3.1e-128
Identity = 265/560 (47.32%), Postives = 363/560 (64.82%), Query Frame = 0

Query: 9   NAAPHSIDLRSGFCPLTKIFHSLRPPLSLPPLSQPLSVTQHAFSLLQSSPLPPNSTLLID 68
           N++   +D RSG+C    IF+S R P+ LP   Q  SV    F    SS         ID
Sbjct: 3   NSSKAIVDERSGYCKSNSIFYSKREPVQLP---QNHSVDVTTFI---SSRAHHGKIAFID 62

Query: 69  SNSGLHLSYAIFLRQIRNLASNLKSLTSLCNGHVAFILAPTSLQIPVLYFALLSLGVVLS 128
           + +G HL++    R + ++A+ L ++  +  G V  +L+P S+  PV+  A++SLG +++
Sbjct: 63  AATGRHLTFPQLWRAVDSVATCLSAM-GIRKGDVILLLSPNSIYFPVVCLAVMSLGAIIT 122

Query: 129 PANPTSSVSEISHQIQLSKPVIAFATSSTASKLPTLRFGTVIIDSPHFLSMLTETNGSDG 188
             NP ++  EI+ QI  SKPV+AF      SK+       VIID     S+    N    
Sbjct: 123 TTNPLNTPREIAKQITDSKPVLAFTIPQLVSKIAGSNLPIVIIDDEVKSSLEKTLNIVSS 182

Query: 189 LTDI------------KIDQSDSAAILYSSGTTGRVKGVLLSHRNLIA-VNSGPTAFQSE 248
           L ++            +++Q D+A +LYSSGTTG  KGV+ SH+NLIA V +  + F +E
Sbjct: 183 LGEMMRKEPSPNRIGYRVNQEDTATLLYSSGTTGASKGVVSSHKNLIAMVQTILSRFGTE 242

Query: 249 IHEGEMKPHPVALCLLPLFHVFGFVMLVRA-ISRGETLVLMERFEFEGMLRAVEKFRVIY 308
             +GE       +C +P+FH++G        +S G T+V++ +FE   ML A+EK+R  Y
Sbjct: 243 --DGE----HTFICTVPMFHIYGLAAFAMGLLSSGSTIVILSKFEIHEMLSAIEKYRATY 302

Query: 309 IPVSPPLVVAMAK--SDLVAKYDLSSLQILGCGGAPLGKEVIDKFHQKLPSVEIAQGYGL 368
           +P+ PP+++A+ K  + + AKYDLSSLQ +  GGAPL KEVI+ F +  P+V I QGYGL
Sbjct: 303 LPLVPPILMALLKNANHIRAKYDLSSLQSVLSGGAPLSKEVIEGFVENYPTVSILQGYGL 362

Query: 369 TESTAGAARTMEPEEISNTKSVGRLSGSMEAKIVDPASGEALLPNHKGELWLRGPTIMKG 428
           TEST   A T   +E     + G LS SMEAKIV+P +GEAL  N  GELWLRGPTIMKG
Sbjct: 363 TESTGIGASTDCLQESRRYGTAGMLSPSMEAKIVNPETGEALSVNRTGELWLRGPTIMKG 422

Query: 429 YVGDDKATTETLDPDGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELEHLLQS 488
           Y  +++AT+ T+D +GWL+TGDLCY D DGF+++VDRLKELIKYK YQV PAELE LL S
Sbjct: 423 YFSNEEATSSTIDSEGWLRTGDLCYIDEDGFIFVVDRLKELIKYKGYQVAPAELEALLLS 482

Query: 489 NSEIIDAAVIPYPDEEAGEIPMAYVVRKPGSNISEAQVIDFIAKQVAPYKKIRRVSFIDA 548
           + EI DAAVIPYPD+EAG+ PMAYVVRK GSN+SE+ V+DFIAK VAPYK+IR+V+F+ +
Sbjct: 483 HPEISDAAVIPYPDKEAGQFPMAYVVRKGGSNLSESTVMDFIAKLVAPYKRIRKVAFVAS 542

Query: 549 IPKSPAGKILRRELAKHALS 553
           IPK+P+GKILR++L K A S
Sbjct: 543 IPKNPSGKILRKDLIKLATS 549

BLAST of Cmc12g0334251 vs. ExPASy TrEMBL
Match: A0A1S4DV63 (4-coumarate--CoA ligase-like 9 OS=Cucumis melo OX=3656 GN=LOC103489008 PE=4 SV=1)

HSP 1 Score: 1077.4 bits (2785), Expect = 0.0e+00
Identity = 555/558 (99.46%), Postives = 555/558 (99.46%), Query Frame = 0

Query: 1   MAARNSNFNAAPHSIDLRSGFCPLTKIFHSLRPPLSLPPLSQPLSVTQHAFSLLQSSPLP 60
           MAARNSNFNAAPHSIDLRSGFCPLTKIFHSLRPPLSLPPLSQPLSVTQHAFSLLQSS  P
Sbjct: 1   MAARNSNFNAAPHSIDLRSGFCPLTKIFHSLRPPLSLPPLSQPLSVTQHAFSLLQSSSPP 60

Query: 61  PNSTLLIDSNSGLHLSYAIFLRQIRNLASNLKSLTSLCNGHVAFILAPTSLQIPVLYFAL 120
           PNSTLLIDSNSGLHLSYAIFLRQIRNLASNLKSLTSLCNGHVAFILAPTSLQIPVLYFAL
Sbjct: 61  PNSTLLIDSNSGLHLSYAIFLRQIRNLASNLKSLTSLCNGHVAFILAPTSLQIPVLYFAL 120

Query: 121 LSLGVVLSPANPTSSVSEISHQIQLSKPVIAFATSSTASKLPTLRFGTVIIDSPHFLSML 180
           LSLGVVLSPANPTSSVSEISHQIQLSKPVIAFATSSTASKLPTLRFGTVIIDSPHFLSML
Sbjct: 121 LSLGVVLSPANPTSSVSEISHQIQLSKPVIAFATSSTASKLPTLRFGTVIIDSPHFLSML 180

Query: 181 TETNGSDGLTDIKIDQSDSAAILYSSGTTGRVKGVLLSHRNLIAVNSGPTAFQSEIHEGE 240
           TETNGSDGLTDIKIDQSDSAAILYSSGTTGRVKGVLLSHRNLIAVNSGPTAFQSEIHEGE
Sbjct: 181 TETNGSDGLTDIKIDQSDSAAILYSSGTTGRVKGVLLSHRNLIAVNSGPTAFQSEIHEGE 240

Query: 241 MKPHPVALCLLPLFHVFGFVMLVRAISRGETLVLMERFEFEGMLRAVEKFRVIYIPVSPP 300
           MKPHPVALCLLPLFHVFGFVMLVRAISRGETLVLMERFEFEGMLRAVEKFRVIYIPVSPP
Sbjct: 241 MKPHPVALCLLPLFHVFGFVMLVRAISRGETLVLMERFEFEGMLRAVEKFRVIYIPVSPP 300

Query: 301 LVVAMAKSDLVAKYDLSSLQILGCGGAPLGKEVIDKFHQKLPSVEIAQGYGLTESTAGAA 360
           LVVAMAKSDLVAKYDLSSLQILGCGGAPLGKEVIDKFHQKLPSVEIAQGYGLTESTAGAA
Sbjct: 301 LVVAMAKSDLVAKYDLSSLQILGCGGAPLGKEVIDKFHQKLPSVEIAQGYGLTESTAGAA 360

Query: 361 RTMEPEEISNTKSVGRLSGSMEAKIVDPASGEALLPNHKGELWLRGPTIMKGYVGDDKAT 420
           RTMEPEEISNTKSVGRLSGSMEAKIVDPASGEALLPNHKGELWLRGPTIMKGYVGDDKAT
Sbjct: 361 RTMEPEEISNTKSVGRLSGSMEAKIVDPASGEALLPNHKGELWLRGPTIMKGYVGDDKAT 420

Query: 421 TETLDPDGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELEHLLQSNSEIIDAA 480
           TETLDPDGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELEHLLQSN EIIDAA
Sbjct: 421 TETLDPDGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELEHLLQSNPEIIDAA 480

Query: 481 VIPYPDEEAGEIPMAYVVRKPGSNISEAQVIDFIAKQVAPYKKIRRVSFIDAIPKSPAGK 540
           VIPYPDEEAGEIPMAYVVRKPGSNISEAQVIDFIAKQVAPYKKIRRVSFIDAIPKSPAGK
Sbjct: 481 VIPYPDEEAGEIPMAYVVRKPGSNISEAQVIDFIAKQVAPYKKIRRVSFIDAIPKSPAGK 540

Query: 541 ILRRELAKHALSHGSSKL 559
           ILRRELAKHALSHGSSKL
Sbjct: 541 ILRRELAKHALSHGSSKL 558

BLAST of Cmc12g0334251 vs. ExPASy TrEMBL
Match: A0A5A7UM03 (4-coumarate-CoA ligase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold80G001590 PE=4 SV=1)

HSP 1 Score: 1076.6 bits (2783), Expect = 0.0e+00
Identity = 558/569 (98.07%), Postives = 558/569 (98.07%), Query Frame = 0

Query: 1   MAARNSNFNAAPHSIDLRSGFCPLTKIFHSLRPPLSLPPLSQPLSVTQHAFSLLQSSPLP 60
           MAARNSNFNAAPHSIDLRSGFCPLTKIFHSLRPPLSLPPLSQPLSVTQHAFSLLQSSPLP
Sbjct: 1   MAARNSNFNAAPHSIDLRSGFCPLTKIFHSLRPPLSLPPLSQPLSVTQHAFSLLQSSPLP 60

Query: 61  PNSTLLIDSNSGLHLSYAIFLRQIRNLASNLKSLTSLCNGHVAFILAPTSLQIPVLYFAL 120
           PNSTLLIDSNSGLHLSYAIFLRQIRNLASNLKSLTSLCNGHVAFILAPTSLQIPVLYFAL
Sbjct: 61  PNSTLLIDSNSGLHLSYAIFLRQIRNLASNLKSLTSLCNGHVAFILAPTSLQIPVLYFAL 120

Query: 121 LSLGVVLSPANPTSSVSEISHQIQLSKPVIAFATSSTASKLPTLRFGTVIIDSPHFLSML 180
           LSLGVVLSPANPTSSVSEISHQIQLSKPVIAFATSSTASKLPTLRFGTVIIDSPHFLSML
Sbjct: 121 LSLGVVLSPANPTSSVSEISHQIQLSKPVIAFATSSTASKLPTLRFGTVIIDSPHFLSML 180

Query: 181 TETNGSDGLTDIKIDQSDSAAILYSSGTTGRVKGVLLSHRNLIAVNSGPTAFQSEIHEGE 240
           TETNGSDGLTDIKIDQSDSAAILYSSGTTGRVKGVLLSHRNLIAVNSGPTAFQSEIHEGE
Sbjct: 181 TETNGSDGLTDIKIDQSDSAAILYSSGTTGRVKGVLLSHRNLIAVNSGPTAFQSEIHEGE 240

Query: 241 MKPHPVALCLLPLFHVFGFVMLVRAISRGETLVLMERFEFEGMLRAVEKFRVIYIPVSPP 300
           MKPHPVALCLLPLFHVFGFVMLVRAISRGETLVLMERFEFEGMLRAVEKFRVIYIPVSPP
Sbjct: 241 MKPHPVALCLLPLFHVFGFVMLVRAISRGETLVLMERFEFEGMLRAVEKFRVIYIPVSPP 300

Query: 301 LVVAMAKSDLVAKYDLSSLQILGCGGAPLGKEVIDKFHQKLPSVEIAQGYGLTESTAGAA 360
           LVVAMAKSDLVAKYDLSSLQILGCGGAPLGKEVIDKFHQKLPSVEIAQGYGLTESTAGAA
Sbjct: 301 LVVAMAKSDLVAKYDLSSLQILGCGGAPLGKEVIDKFHQKLPSVEIAQGYGLTESTAGAA 360

Query: 361 RTMEPEEISNTKSVGRLSGSMEAKIVDPASGEALLPNHKGELWLRGPTIMKGYVGDDKAT 420
           RTMEPEEISNTKSVGRLSGSMEAKIVDPASGEALLPNHKGELWLRGPTIMKGYVGDDKAT
Sbjct: 361 RTMEPEEISNTKSVGRLSGSMEAKIVDPASGEALLPNHKGELWLRGPTIMKGYVGDDKAT 420

Query: 421 TETLDPDGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELEHLLQSNSEIIDAA 480
           TETLDPDGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELEHLLQSNSEIIDAA
Sbjct: 421 TETLDPDGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELEHLLQSNSEIIDAA 480

Query: 481 VIPYPDEEAGEIPMAYVVRKPGSNISEAQVIDFIAKQ-----------VAPYKKIRRVSF 540
           VIPYPDEEAGEIPMAYVVRKPGSNISEAQVIDFIAKQ           VAPYKKIRRVSF
Sbjct: 481 VIPYPDEEAGEIPMAYVVRKPGSNISEAQVIDFIAKQAMLHLSRNFQIVAPYKKIRRVSF 540

Query: 541 IDAIPKSPAGKILRRELAKHALSHGSSKL 559
           IDAIPKSPAGKILRRELAKHALSHGSSKL
Sbjct: 541 IDAIPKSPAGKILRRELAKHALSHGSSKL 569

BLAST of Cmc12g0334251 vs. ExPASy TrEMBL
Match: A0A5D3BKJ5 (4-coumarate-CoA ligase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G004520 PE=4 SV=1)

HSP 1 Score: 1068.9 bits (2763), Expect = 7.1e-309
Identity = 555/569 (97.54%), Postives = 555/569 (97.54%), Query Frame = 0

Query: 1   MAARNSNFNAAPHSIDLRSGFCPLTKIFHSLRPPLSLPPLSQPLSVTQHAFSLLQSSPLP 60
           MAARNSNFNAAPHSIDLRSGFCPLTKIFHSLRPPLSLPPLSQPLSVTQHAFSLLQSS  P
Sbjct: 1   MAARNSNFNAAPHSIDLRSGFCPLTKIFHSLRPPLSLPPLSQPLSVTQHAFSLLQSSSPP 60

Query: 61  PNSTLLIDSNSGLHLSYAIFLRQIRNLASNLKSLTSLCNGHVAFILAPTSLQIPVLYFAL 120
           PNSTLLIDSNSGLHLSYAIFLRQIRNLASNLKSLTSLCNGHVAFILAPTSLQIPVLYFAL
Sbjct: 61  PNSTLLIDSNSGLHLSYAIFLRQIRNLASNLKSLTSLCNGHVAFILAPTSLQIPVLYFAL 120

Query: 121 LSLGVVLSPANPTSSVSEISHQIQLSKPVIAFATSSTASKLPTLRFGTVIIDSPHFLSML 180
           LSLGVVLSPANPTSSVSEISHQIQLSKPVIAFATSSTASKLPTLRFGTVIIDSPHFLSML
Sbjct: 121 LSLGVVLSPANPTSSVSEISHQIQLSKPVIAFATSSTASKLPTLRFGTVIIDSPHFLSML 180

Query: 181 TETNGSDGLTDIKIDQSDSAAILYSSGTTGRVKGVLLSHRNLIAVNSGPTAFQSEIHEGE 240
           TETNGSDGLTDIKIDQSDSAAILYSSGTTGRVKGVLLSHRNLIAVNSGPTAFQSEIHEGE
Sbjct: 181 TETNGSDGLTDIKIDQSDSAAILYSSGTTGRVKGVLLSHRNLIAVNSGPTAFQSEIHEGE 240

Query: 241 MKPHPVALCLLPLFHVFGFVMLVRAISRGETLVLMERFEFEGMLRAVEKFRVIYIPVSPP 300
           MKPHPVALCLLPLFHVFGFVMLVRAISRGETLVLMERFEFEGMLRAVEKFRVIYIPVSPP
Sbjct: 241 MKPHPVALCLLPLFHVFGFVMLVRAISRGETLVLMERFEFEGMLRAVEKFRVIYIPVSPP 300

Query: 301 LVVAMAKSDLVAKYDLSSLQILGCGGAPLGKEVIDKFHQKLPSVEIAQGYGLTESTAGAA 360
           LVVAMAKSDLVAKYDLSSLQILGCGGAPLGKEVIDKFHQKLPSVEIAQGYGLTESTAGAA
Sbjct: 301 LVVAMAKSDLVAKYDLSSLQILGCGGAPLGKEVIDKFHQKLPSVEIAQGYGLTESTAGAA 360

Query: 361 RTMEPEEISNTKSVGRLSGSMEAKIVDPASGEALLPNHKGELWLRGPTIMKGYVGDDKAT 420
           RTMEPEEISNTKSVGRLSGSMEAKIVDPASGEALLPNHKGELWLRGPTIMKGYVGDDKAT
Sbjct: 361 RTMEPEEISNTKSVGRLSGSMEAKIVDPASGEALLPNHKGELWLRGPTIMKGYVGDDKAT 420

Query: 421 TETLDPDGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELEHLLQSNSEIIDAA 480
           TETLDPDGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELEHLLQSN EIIDAA
Sbjct: 421 TETLDPDGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELEHLLQSNPEIIDAA 480

Query: 481 VIPYPDEEAGEIPMAYVVRKPGSNISEAQVIDFIAKQ-----------VAPYKKIRRVSF 540
           VIPYPDEEAGEIPMAYVVRKPGSNISEAQVIDFIAKQ           VAPYKKIRRVSF
Sbjct: 481 VIPYPDEEAGEIPMAYVVRKPGSNISEAQVIDFIAKQAMLHLSRNFQIVAPYKKIRRVSF 540

Query: 541 IDAIPKSPAGKILRRELAKHALSHGSSKL 559
           IDAIPKSPAGKILRRELAKHALSHGSSKL
Sbjct: 541 IDAIPKSPAGKILRRELAKHALSHGSSKL 569

BLAST of Cmc12g0334251 vs. ExPASy TrEMBL
Match: E5GBV5 (4-coumarate-CoA ligase OS=Cucumis melo subsp. melo OX=412675 PE=4 SV=1)

HSP 1 Score: 1068.9 bits (2763), Expect = 7.1e-309
Identity = 555/569 (97.54%), Postives = 555/569 (97.54%), Query Frame = 0

Query: 1    MAARNSNFNAAPHSIDLRSGFCPLTKIFHSLRPPLSLPPLSQPLSVTQHAFSLLQSSPLP 60
            MAARNSNFNAAPHSIDLRSGFCPLTKIFHSLRPPLSLPPLSQPLSVTQHAFSLLQSS  P
Sbjct: 487  MAARNSNFNAAPHSIDLRSGFCPLTKIFHSLRPPLSLPPLSQPLSVTQHAFSLLQSSSPP 546

Query: 61   PNSTLLIDSNSGLHLSYAIFLRQIRNLASNLKSLTSLCNGHVAFILAPTSLQIPVLYFAL 120
            PNSTLLIDSNSGLHLSYAIFLRQIRNLASNLKSLTSLCNGHVAFILAPTSLQIPVLYFAL
Sbjct: 547  PNSTLLIDSNSGLHLSYAIFLRQIRNLASNLKSLTSLCNGHVAFILAPTSLQIPVLYFAL 606

Query: 121  LSLGVVLSPANPTSSVSEISHQIQLSKPVIAFATSSTASKLPTLRFGTVIIDSPHFLSML 180
            LSLGVVLSPANPTSSVSEISHQIQLSKPVIAFATSSTASKLPTLRFGTVIIDSPHFLSML
Sbjct: 607  LSLGVVLSPANPTSSVSEISHQIQLSKPVIAFATSSTASKLPTLRFGTVIIDSPHFLSML 666

Query: 181  TETNGSDGLTDIKIDQSDSAAILYSSGTTGRVKGVLLSHRNLIAVNSGPTAFQSEIHEGE 240
            TETNGSDGLTDIKIDQSDSAAILYSSGTTGRVKGVLLSHRNLIAVNSGPTAFQSEIHEGE
Sbjct: 667  TETNGSDGLTDIKIDQSDSAAILYSSGTTGRVKGVLLSHRNLIAVNSGPTAFQSEIHEGE 726

Query: 241  MKPHPVALCLLPLFHVFGFVMLVRAISRGETLVLMERFEFEGMLRAVEKFRVIYIPVSPP 300
            MKPHPVALCLLPLFHVFGFVMLVRAISRGETLVLMERFEFEGMLRAVEKFRVIYIPVSPP
Sbjct: 727  MKPHPVALCLLPLFHVFGFVMLVRAISRGETLVLMERFEFEGMLRAVEKFRVIYIPVSPP 786

Query: 301  LVVAMAKSDLVAKYDLSSLQILGCGGAPLGKEVIDKFHQKLPSVEIAQGYGLTESTAGAA 360
            LVVAMAKSDLVAKYDLSSLQILGCGGAPLGKEVIDKFHQKLPSVEIAQGYGLTESTAGAA
Sbjct: 787  LVVAMAKSDLVAKYDLSSLQILGCGGAPLGKEVIDKFHQKLPSVEIAQGYGLTESTAGAA 846

Query: 361  RTMEPEEISNTKSVGRLSGSMEAKIVDPASGEALLPNHKGELWLRGPTIMKGYVGDDKAT 420
            RTMEPEEISNTKSVGRLSGSMEAKIVDPASGEALLPNHKGELWLRGPTIMKGYVGDDKAT
Sbjct: 847  RTMEPEEISNTKSVGRLSGSMEAKIVDPASGEALLPNHKGELWLRGPTIMKGYVGDDKAT 906

Query: 421  TETLDPDGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELEHLLQSNSEIIDAA 480
            TETLDPDGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELEHLLQSN EIIDAA
Sbjct: 907  TETLDPDGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELEHLLQSNPEIIDAA 966

Query: 481  VIPYPDEEAGEIPMAYVVRKPGSNISEAQVIDFIAKQ-----------VAPYKKIRRVSF 540
            VIPYPDEEAGEIPMAYVVRKPGSNISEAQVIDFIAKQ           VAPYKKIRRVSF
Sbjct: 967  VIPYPDEEAGEIPMAYVVRKPGSNISEAQVIDFIAKQAMLHLSRNFQIVAPYKKIRRVSF 1026

Query: 541  IDAIPKSPAGKILRRELAKHALSHGSSKL 559
            IDAIPKSPAGKILRRELAKHALSHGSSKL
Sbjct: 1027 IDAIPKSPAGKILRRELAKHALSHGSSKL 1055

BLAST of Cmc12g0334251 vs. ExPASy TrEMBL
Match: A0A0A0LTS5 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G050280 PE=4 SV=1)

HSP 1 Score: 1011.9 bits (2615), Expect = 1.0e-291
Identity = 515/558 (92.29%), Postives = 534/558 (95.70%), Query Frame = 0

Query: 1   MAARNSNFNAAPHSIDLRSGFCPLTKIFHSLRPPLSLPPLSQPLSVTQHAFSLLQSSPLP 60
           MA RNSNF  +PHSIDLRSGFCP TKIFHSLRPPLSLPPLS PLSV QHAFSLLQSSP P
Sbjct: 1   MADRNSNFTTSPHSIDLRSGFCPQTKIFHSLRPPLSLPPLSHPLSVIQHAFSLLQSSPPP 60

Query: 61  PNSTLLIDSNSGLHLSYAIFLRQIRNLASNLKSLTSLCNGHVAFILAPTSLQIPVLYFAL 120
           PNSTLLID NSGLHLSY +FLRQIRNLASNLKSLTSLCNG VAFILAPTSLQIPVLYFAL
Sbjct: 61  PNSTLLIDFNSGLHLSYTVFLRQIRNLASNLKSLTSLCNGQVAFILAPTSLQIPVLYFAL 120

Query: 121 LSLGVVLSPANPTSSVSEISHQIQLSKPVIAFATSSTASKLPTLRFGTVIIDSPHFLSML 180
           LSLGVVLSPANPTSSVSEISHQIQLSKPVIAFATSSTASKLP LRFGTV+IDSPHF SML
Sbjct: 121 LSLGVVLSPANPTSSVSEISHQIQLSKPVIAFATSSTASKLPKLRFGTVLIDSPHFHSML 180

Query: 181 TETNGSDGLTDIKIDQSDSAAILYSSGTTGRVKGVLLSHRNLIAVNSGPTAFQSEIHEGE 240
           TETNGSDG+ +IKIDQSDSAAILYSSGTTGRVKGVLLSHRNLIAVN+GP + Q EI EGE
Sbjct: 181 TETNGSDGIPNIKIDQSDSAAILYSSGTTGRVKGVLLSHRNLIAVNAGPNSLQLEIQEGE 240

Query: 241 MKPHPVALCLLPLFHVFGFVMLVRAISRGETLVLMERFEFEGMLRAVEKFRVIYIPVSPP 300
           M+PHPV LC LPLFH+FGFVMLVRA+SRG+TLVLMERFEFEGMLRAVEK+RV YIPVSPP
Sbjct: 241 MEPHPVVLCPLPLFHIFGFVMLVRAVSRGDTLVLMERFEFEGMLRAVEKYRVKYIPVSPP 300

Query: 301 LVVAMAKSDLVAKYDLSSLQILGCGGAPLGKEVIDKFHQKLPSVEIAQGYGLTESTAGAA 360
           LVVAMAKS+LV KYDLSSLQ+LGCGGAPLGKEVIDKFHQKLP VEI QGYGLTESTAGA+
Sbjct: 301 LVVAMAKSELVGKYDLSSLQMLGCGGAPLGKEVIDKFHQKLPGVEIVQGYGLTESTAGAS 360

Query: 361 RTMEPEEISNTKSVGRLSGSMEAKIVDPASGEALLPNHKGELWLRGPTIMKGYVGDDKAT 420
           RTM+PEEISNTKSVGRLSG+MEAKIVDPASG+ALLPNHKGELWLRGPTIMKGYVGDDKAT
Sbjct: 361 RTMDPEEISNTKSVGRLSGNMEAKIVDPASGDALLPNHKGELWLRGPTIMKGYVGDDKAT 420

Query: 421 TETLDPDGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELEHLLQSNSEIIDAA 480
           TETLDPDGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELEHLLQSN EIIDAA
Sbjct: 421 TETLDPDGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELEHLLQSNPEIIDAA 480

Query: 481 VIPYPDEEAGEIPMAYVVRKPGSNISEAQVIDFIAKQVAPYKKIRRVSFIDAIPKSPAGK 540
           VIPYPDEEAGEIPMAYVVRKPGSN+SEAQVIDFIAKQVAPYKKIRRVSFIDAIPKSPAGK
Sbjct: 481 VIPYPDEEAGEIPMAYVVRKPGSNMSEAQVIDFIAKQVAPYKKIRRVSFIDAIPKSPAGK 540

Query: 541 ILRRELAKHALSHGSSKL 559
           ILRRELAKHALSHGSSKL
Sbjct: 541 ILRRELAKHALSHGSSKL 558

BLAST of Cmc12g0334251 vs. TAIR 10
Match: AT5G63380.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 644.4 bits (1661), Expect = 8.2e-185
Identity = 340/555 (61.26%), Postives = 429/555 (77.30%), Query Frame = 0

Query: 15  IDLRSGFCPLTKIFHSLRPPLSLPPLSQPLSVTQHAFSLLQSSPLPPNS-------TLLI 74
           ID  SGF   T I+HSLRP LSLPP+ QPLS  + A SLL  S  P  +       T L+
Sbjct: 11  IDRSSGFDQRTGIYHSLRPSLSLPPIDQPLSAAEFALSLLLKSSPPATAGKNIEALTYLV 70

Query: 75  DSNSGLHLSYAIFLRQIRNLASNLKS-LTSLCNGHVAFILAPTSLQIPVLYFALLSLGVV 134
           +S+SG +L+Y   LR++R+LA +L+    SL + +VAFIL+P+SL IPVLY AL+S+GVV
Sbjct: 71  NSSSGDNLTYGELLRRVRSLAVSLRERFPSLASRNVAFILSPSSLDIPVLYLALMSIGVV 130

Query: 135 LSPANPTSSVSEISHQIQLSKPVIAFATSSTASKL--PTLRFGTVIIDSPHFLSMLTETN 194
           +SPANP  S SE+SHQ+++S+PVIAFATS T  KL   +L  GTV++DS  FLS L  ++
Sbjct: 131 VSPANPIGSESEVSHQVEVSEPVIAFATSQTVKKLQSSSLPLGTVLMDSTEFLSWLNRSD 190

Query: 195 GSD-GLTDIKIDQSDSAAILYSSGTTGRVKGVLLSHRNLIAVNSGPTAFQSEIHEGEMKP 254
            S      ++++QSD AAIL+SSGTTGRVKGVLL+HRNLIA  S   + Q  + +  +  
Sbjct: 191 SSSVNPFQVQVNQSDPAAILFSSGTTGRVKGVLLTHRNLIA--STAVSHQRTLQD-PVNY 250

Query: 255 HPVALCLLPLFHVFGFVMLVRAISRGETLVLMERFEFEGMLRAVEKFRVIYIPVSPPLVV 314
             V L  LPLFHVFGF+M++RAIS GETLVL+ RFE E M +AVEK++V  +PVSPPL+V
Sbjct: 251 DRVGLFSLPLFHVFGFMMMIRAISLGETLVLLGRFELEAMFKAVEKYKVTGMPVSPPLIV 310

Query: 315 AMAKSDLVAKYDLSSLQILGCGGAPLGKEVIDKFHQKLPSVEIAQGYGLTESTAGAARTM 374
           A+ KS+L  KYDL SL+ LGCGGAPLGK++ ++F QK P V+I QGYGLTES+  AA T 
Sbjct: 311 ALVKSELTKKYDLRSLRSLGCGGAPLGKDIAERFKQKFPDVDIVQGYGLTESSGPAASTF 370

Query: 375 EPEEISNTKSVGRLSGSMEAKIVDPASGEALLPNHKGELWLRGPTIMKGYVGDDKATTET 434
            PEE+    SVGR+S +MEAKIVDP++GE+L P   GELWLRGP IMKGYVG++KA+ ET
Sbjct: 371 GPEEMVKYGSVGRISENMEAKIVDPSTGESLPPGKTGELWLRGPVIMKGYVGNEKASAET 430

Query: 435 LDPDGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELEHLLQSNSEIIDAAVIP 494
           +D +GWLKTGDLCYFDS+ FLYIVDRLKELIKYKAYQVPP ELE +L SN ++IDAAV+P
Sbjct: 431 VDKEGWLKTGDLCYFDSEDFLYIVDRLKELIKYKAYQVPPVELEQILHSNPDVIDAAVVP 490

Query: 495 YPDEEAGEIPMAYVVRKPGSNISEAQVIDFIAKQVAPYKKIRRVSFIDAIPKSPAGKILR 554
           +PDE+AGEIPMA++VRKPGSN++EAQ+IDF+AKQV PYKK+RRV+FI+AIPK+PAGKILR
Sbjct: 491 FPDEDAGEIPMAFIVRKPGSNLNEAQIIDFVAKQVTPYKKVRRVAFINAIPKNPAGKILR 550

Query: 555 RELAKHALSHGSSKL 559
           REL K A+   +SKL
Sbjct: 551 RELTKIAVDGNASKL 562

BLAST of Cmc12g0334251 vs. TAIR 10
Match: AT1G20510.1 (OPC-8:0 CoA ligase1 )

HSP 1 Score: 457.2 bits (1175), Expect = 1.9e-128
Identity = 251/554 (45.31%), Postives = 360/554 (64.98%), Query Frame = 0

Query: 14  SIDLRSGFCPLTKIFHSLRPPLSLPPLSQPLSVTQHAFSLLQSSPLPPNSTLLIDSNSGL 73
           S++ RSGFC     F+S R P+ LPP +  L VT        SS         ID+++G 
Sbjct: 3   SVNSRSGFCNSNSTFYSKRTPIPLPP-NPSLDVTTFI-----SSQAHRGRIAFIDASTGQ 62

Query: 74  HLSYAIFLRQIRNLASNLKSLTSLCNGHVAFILAPTSLQIPVLYFALLSLGVVLSPANPT 133
           +L++    R + ++A  L  +  +  GHV  +L+P S+  PV+  +++SLG +++  NP 
Sbjct: 63  NLTFTELWRAVESVADCLSEI-GIRKGHVVLLLSPNSILFPVVCLSVMSLGAIITTTNPL 122

Query: 134 SSVSEISHQIQLSKPVIAFAT-------SSTASKLPTLRFGTVIIDSPHFLSMLTE---T 193
           ++ +EI+ QI+ S PV+AF T       S+ A KLP +      +DS   +  L E    
Sbjct: 123 NTSNEIAKQIKDSNPVLAFTTSQLLPKISAAAKKLPIVLMDEERVDSVGDVRRLVEMMKK 182

Query: 194 NGSDGLTDIKIDQSDSAAILYSSGTTGRVKGVLLSHRNLIA-VNSGPTAFQSEIHEGEMK 253
             S      ++DQ D+A +LYSSGTTG  KGV+ SHRNLIA V +    F S+  +GE +
Sbjct: 183 EPSGNRVKERVDQDDTATLLYSSGTTGMSKGVISSHRNLIAMVQTIVNRFGSD--DGEQR 242

Query: 254 PHPVALCLLPLFHVFGFVMLVRA-ISRGETLVLMERFEFEGMLRAVEKFRVIYIPVSPPL 313
                +C +P+FH++G        ++ G T++++ +FE   M+ A+ K++   +P+ PP+
Sbjct: 243 ----FICTVPMFHIYGLAAFATGLLAYGSTIIVLSKFEMHEMMSAIGKYQATSLPLVPPI 302

Query: 314 VVAMAK--SDLVAKYDLSSLQILGCGGAPLGKEVIDKFHQKLPSVEIAQGYGLTESTAGA 373
           +VAM      + AKYDLSS+  + CGGAPL KEV + F +K P+V+I QGYGLTEST   
Sbjct: 303 LVAMVNGADQIKAKYDLSSMHTVLCGGAPLSKEVTEGFAEKYPTVKILQGYGLTESTGIG 362

Query: 374 ARTMEPEEISNTKSVGRLSGSMEAKIVDPASGEALLPNHKGELWLRGPTIMKGYVGDDKA 433
           A T   EE     + G+LS SME +IVDP +G+ L P   GELWL+GP+IMKGY  +++A
Sbjct: 363 ASTDTVEESRRYGTAGKLSASMEGRIVDPVTGQILGPKQTGELWLKGPSIMKGYFSNEEA 422

Query: 434 TTETLDPDGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELEHLLQSNSEIIDA 493
           T+ TLD +GWL+TGDLCY D DGF+++VDRLKELIKYK YQV PAELE LL ++ EI DA
Sbjct: 423 TSSTLDSEGWLRTGDLCYIDEDGFIFVVDRLKELIKYKGYQVAPAELEALLLTHPEITDA 482

Query: 494 AVIPYPDEEAGEIPMAYVVRKPGSNISEAQVIDFIAKQVAPYKKIRRVSFIDAIPKSPAG 553
           AVIP+PD+E G+ PMAYVVRK GS++SE  +++F+AKQVAPYK+IR+V+F+ +IPK+P+G
Sbjct: 483 AVIPFPDKEVGQFPMAYVVRKTGSSLSEKTIMEFVAKQVAPYKRIRKVAFVSSIPKNPSG 542

BLAST of Cmc12g0334251 vs. TAIR 10
Match: AT1G20500.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 448.0 bits (1151), Expect = 1.1e-125
Identity = 254/553 (45.93%), Postives = 351/553 (63.47%), Query Frame = 0

Query: 15  IDLRSGFCPLTKIFHSLRPPLSLPPLSQPLSVTQHAFSLLQSSPLPPNSTLLIDSNSGLH 74
           +D RSGFC     F+S R PLSLPP     ++++   + + S P     T  ID+ +G  
Sbjct: 10  VDPRSGFCKSNSTFYSKRQPLSLPP-----NLSRDVTTFISSQP-HRGKTAFIDAATGQC 69

Query: 75  LSYAIFLRQIRNLASNLKSLTSLCNGHVAFILAPTSLQIPVLYFALLSLGVVLSPANPTS 134
           L+++   R +  +A  L     +  G V  IL+P S+ IPV+  +++SLG V + AN  +
Sbjct: 70  LTFSDLWRAVDRVADCLYHEVGIRRGDVVLILSPNSIFIPVVCLSVMSLGAVFTTANTLN 129

Query: 135 SVSEISHQIQLSKPVIAFATSSTASKLPTLRFGTVIIDSPHF------------LSMLTE 194
           +  EIS QI  S P + F T   A KLP +    V+ D   +            LS + +
Sbjct: 130 TSGEISKQIADSNPTLVFTTRQLAPKLP-VAISVVLTDDEVYQELTSAIRVVGILSEMVK 189

Query: 195 TNGSDGLTDIKIDQSDSAAILYSSGTTGRVKGVLLSHRNLIAVNSGPTAFQSEIHEGEMK 254
              S      +++Q D+A +LYSSGTTG  KGV+ SHRNL       TA  +      +K
Sbjct: 190 KEPSGQRVRDRVNQDDTAMMLYSSGTTGPSKGVISSHRNL-------TAHVARFISDNLK 249

Query: 255 PHPVALCLLPLFHVFGFV-MLVRAISRGETLVLMERFEFEGMLRAVEKFRVIYIPVSPPL 314
              + +C +P+FH +G +   +  ++ G T+V++ RF+   M+ AVEK R   + ++PP+
Sbjct: 250 RDDIFICTVPMFHTYGLLTFAMGTVALGSTVVILRRFQLHDMMDAVEKHRATALALAPPV 309

Query: 315 VVAMAK-SDLV-AKYDLSSLQILGCGGAPLGKEVIDKFHQKLPSVEIAQGYGLTESTAGA 374
           +VAM   +DL+ AKYDLSSL+ + CGGAPL KEV + F +K P+V+I QGY LTES  G 
Sbjct: 310 LVAMINDADLIKAKYDLSSLKTVRCGGAPLSKEVTEGFLEKYPTVDILQGYALTESNGGG 369

Query: 375 ARTMEPEEISNTKSVGRLSGSMEAKIVDPASGEALLPNHKGELWLRGPTIMKGYVGDDKA 434
           A T   EE     + G L+  +EA+IVDP +G  +  N  GELWL+GP+I KGY  + +A
Sbjct: 370 AFTNSAEESRRYGTAGTLTSDVEARIVDPNTGRFMGINQTGELWLKGPSISKGYFKNQEA 429

Query: 435 TTETLDPDGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELEHLLQSNSEIIDA 494
           T ET++ +GWLKTGDLCY D DGFL++VDRLKELIKYK YQVPPAELE LL ++ +I+DA
Sbjct: 430 TNETINLEGWLKTGDLCYIDEDGFLFVVDRLKELIKYKGYQVPPAELEALLITHPDILDA 489

Query: 495 AVIPYPDEEAGEIPMAYVVRKPGSNISEAQVIDFIAKQVAPYKKIRRVSFIDAIPKSPAG 553
           AVIP+PD+EAG+ PMAYVVRK  SN+SE QVIDFI+KQVAPYKKIR+VSFI++IPK+ +G
Sbjct: 490 AVIPFPDKEAGQYPMAYVVRKHESNLSEKQVIDFISKQVAPYKKIRKVSFINSIPKTASG 548

BLAST of Cmc12g0334251 vs. TAIR 10
Match: AT1G20480.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 441.4 bits (1134), Expect = 1.1e-123
Identity = 251/555 (45.23%), Postives = 351/555 (63.24%), Query Frame = 0

Query: 14  SIDLRSGFCPLTKIFHSLRPPLSLPPLSQPLSVTQHAFSLLQSSPLPPNSTLLIDSNSGL 73
           ++D +SGFC  T IF+S R P++LPP +Q L VT    S + S P     T+ +D+ +G 
Sbjct: 20  AVDRKSGFCESTSIFYSKREPMALPP-NQFLDVT----SFIASQP-HRGKTVFVDAVTGR 79

Query: 74  HLSYAIFLRQIRNLASNLKSLTSLCNGHVAFILAPTSLQIPVLYFALLSLGVVLSPANPT 133
            LS+      +  +A  L +L  +  G+V  IL+P S+  P++  +++SLG +++ ANP 
Sbjct: 80  RLSFPELWLGVERVAGCLYAL-GVRKGNVVIILSPNSILFPIVSLSVMSLGAIITTANPI 139

Query: 134 SSVSEISHQIQLSKPVIAFATSSTASKL---PTLRFGTVIIDSPHF-------------- 193
           ++  EIS QI  S+PV+AF T    SKL          V++D  H               
Sbjct: 140 NTSDEISKQIGDSRPVLAFTTCKLVSKLAAASNFNLPVVLMDDYHVPSQSYGDRVKLVGR 199

Query: 194 LSMLTETNGSDGLTDIKIDQSDSAAILYSSGTTGRVKGVLLSHRNLIAVNSGPTAFQSEI 253
           L  + ET  S+     +++Q D+AA+LYSSGTTG  KGV+LSHRNLIA+         + 
Sbjct: 200 LETMIETEPSESRVKQRVNQDDTAALLYSSGTTGTSKGVMLSHRNLIAL--------VQA 259

Query: 254 HEGEMKPHPVALCLLPLFHVFGF-VMLVRAISRGETLVLMERFEFEGMLRAVEKFRVIYI 313
           +          +C +P+ H+FGF       I+ G T+V++ +F+   +L AVE  R  Y+
Sbjct: 260 YRARFGLEQRTICTIPMCHIFGFGGFATGLIALGWTIVVLPKFDMAKLLSAVETHRSSYL 319

Query: 314 PVSPPLVVAMAK--SDLVAKYDLSSLQILGCGGAPLGKEVIDKFHQKLPSVEIAQGYGLT 373
            + PP+VVAM    +++ +KYDLSSL  +  GGAPL +EV +KF +  P V+I QGYGLT
Sbjct: 320 SLVPPIVVAMVNGANEINSKYDLSSLHTVVAGGAPLSREVTEKFVENYPKVKILQGYGLT 379

Query: 374 ESTAGAARTMEPEEISNTKSVGRLSGSMEAKIVDPASGEALLPNHKGELWLRGPTIMKGY 433
           ESTA AA     EE     + G L+ ++E KIVDP +G  L  N  GELW+R PT+MKGY
Sbjct: 380 ESTAIAASMFNKEETKRYGASGLLAPNVEGKIVDPDTGRVLGVNQTGELWIRSPTVMKGY 439

Query: 434 VGDDKATTETLDPDGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELEHLLQSN 493
             + +AT  T+D +GWLKTGDLCY D DGF+++VDRLKELIK   YQV PAELE LL ++
Sbjct: 440 FKNKEATASTIDSEGWLKTGDLCYIDGDGFVFVVDRLKELIKCNGYQVAPAELEALLLAH 499

Query: 494 SEIIDAAVIPYPDEEAGEIPMAYVVRKPGSNISEAQVIDFIAKQVAPYKKIRRVSFIDAI 549
            EI DAAVIP PD +AG+ PMAY+VRK GSN+SE++++ F+AKQV+PYKKIR+V+F+ +I
Sbjct: 500 PEIADAAVIPIPDMKAGQYPMAYIVRKVGSNLSESEIMGFVAKQVSPYKKIRKVTFLASI 559

BLAST of Cmc12g0334251 vs. TAIR 10
Match: AT5G38120.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 421.0 bits (1081), Expect = 1.5e-117
Identity = 247/562 (43.95%), Postives = 345/562 (61.39%), Query Frame = 0

Query: 6   SNFNAAPHSIDLRSGFCPLTKIFHSLRPPLSLPPLSQPLSVTQHAFSLLQSSPLPPNSTL 65
           +N   +   ID R+GFC     F+S R PL+LP   + L +T        SS      T 
Sbjct: 2   ANSQRSSSLIDPRNGFCTSNSTFYSKRKPLALPS-KESLDITTFI-----SSQTYRGKTA 61

Query: 66  LIDSNSGLHLSYAIFLRQIRNLASNLKSLTSLCNGHVAFILAPTSLQIPVLYFALLSLGV 125
            ID+ +   +S++     +  +A  L     +  G V  +L+P ++ IP++  +++SLG 
Sbjct: 62  FIDAATDHRISFSDLWMAVDRVADCLLHDVGIRRGDVVLVLSPNTISIPIVCLSVMSLGA 121

Query: 126 VLSPANPTSSVSEISHQIQLSKPVIAFATSSTASKLPTLRFGTVIIDSPHFLSMLTETNG 185
           VL+ ANP ++ SEI  QI  S P +AF T   A K+ +     V+      L +      
Sbjct: 122 VLTTANPLNTASEILRQIADSNPKLAFTTPELAPKIASSGISIVLERVEDTLRVPRGLKV 181

Query: 186 SDGLTDI------------KIDQSDSAAILYSSGTTGRVKGVLLSHRNLIAVNSGPTAFQ 245
              LT++            ++ + D+A +LYSSGTTGR KGV  SH NLIA         
Sbjct: 182 VGNLTEMMKKEPSGQAVRNQVHKDDTAMLLYSSGTTGRSKGVNSSHGNLIA------HVA 241

Query: 246 SEIHEGEMKPHPVALCLLPLFHVFGFVMLVRA-ISRGETLVLMERFEFEGMLRAVEKFRV 305
             I E   +P    +C +PLFH FG +  V A ++ G T+V++ RF+   M+ AVEK+R 
Sbjct: 242 RYIAEPFEQPQQTFICTVPLFHTFGLLNFVLATLALGTTVVILPRFDLGEMMAAVEKYRA 301

Query: 306 IYIPVSPPLVVAMA-KSD-LVAKYDLSSLQILGCGGAPLGKEVIDKFHQKLPSVEIAQGY 365
             + + PP++V M  K+D ++ KYD+S L+ + CGGAPL KEV   F +K P+V++ QGY
Sbjct: 302 TTLILVPPVLVTMINKADQIMKKYDVSFLRTVRCGGAPLSKEVTQGFMKKYPTVDVYQGY 361

Query: 366 GLTESTAGAARTMEPEEISNTKSVGRLSGSMEAKIVDPASGEALLPNHKGELWLRGPTIM 425
            LTES    A     EE     +VG LS  +EA+IVDP +G+ +  N  GELWL+GP+I 
Sbjct: 362 ALTESNGAGASIESVEESRRYGAVGLLSCGVEARIVDPNTGQVMGLNQTGELWLKGPSIA 421

Query: 426 KGYVGDDKATTETLDPDGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELEHLL 485
           KGY  +++   E +  +GWLKTGDLCY D+DGFL+IVDRLKELIKYK YQVPPAELE LL
Sbjct: 422 KGYFRNEE---EIITSEGWLKTGDLCYIDNDGFLFIVDRLKELIKYKGYQVPPAELEALL 481

Query: 486 QSNSEIIDAAVIPYPDEEAGEIPMAYVVRKPGSNISEAQVIDFIAKQVAPYKKIRRVSFI 545
            ++ +I+DAAVIP+PD+EAG+ PMAYV RKP SN+ E +VIDFI+KQVAPYKKIR+V+FI
Sbjct: 482 LNHPDILDAAVIPFPDKEAGQFPMAYVARKPESNLCEKKVIDFISKQVAPYKKIRKVAFI 541

Query: 546 DAIPKSPAGKILRRELAKHALS 553
           D+IPK+P+GK LR++L K A+S
Sbjct: 542 DSIPKTPSGKTLRKDLIKFAIS 548

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_016899600.10.0e+0099.46PREDICTED: 4-coumarate--CoA ligase-like 9 [Cucumis melo][more]
KAA0055316.10.0e+0098.074-coumarate-CoA ligase [Cucumis melo var. makuwa][more]
TYJ99241.11.5e-30897.544-coumarate-CoA ligase [Cucumis melo var. makuwa][more]
ADN33954.11.5e-30897.544-coumarate-CoA ligase [Cucumis melo subsp. melo][more]
XP_011652003.12.1e-29192.294-coumarate--CoA ligase-like 9 [Cucumis sativus] >KGN64389.1 hypothetical protei... [more]
Match NameE-valueIdentityDescription
Q84P231.2e-18361.264-coumarate--CoA ligase-like 9 OS=Arabidopsis thaliana OX=3702 GN=4CLL9 PE=1 SV=... [more]
Q7F1X57.1e-15754.054-coumarate--CoA ligase-like 5 OS=Oryza sativa subsp. japonica OX=39947 GN=4CLL5... [more]
Q8RU951.4e-15252.604-coumarate--CoA ligase-like 6 OS=Oryza sativa subsp. japonica OX=39947 GN=4CLL6... [more]
Q69RG72.1e-13748.224-coumarate--CoA ligase-like 7 OS=Oryza sativa subsp. japonica OX=39947 GN=4CLL7... [more]
M4IQR73.1e-12847.32Probable CoA ligase CCL5 OS=Humulus lupulus OX=3486 GN=CCL5 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A1S4DV630.0e+0099.464-coumarate--CoA ligase-like 9 OS=Cucumis melo OX=3656 GN=LOC103489008 PE=4 SV=1[more]
A0A5A7UM030.0e+0098.074-coumarate-CoA ligase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold8... [more]
A0A5D3BKJ57.1e-30997.544-coumarate-CoA ligase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold2... [more]
E5GBV57.1e-30997.544-coumarate-CoA ligase OS=Cucumis melo subsp. melo OX=412675 PE=4 SV=1[more]
A0A0A0LTS51.0e-29192.29Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G050280 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT5G63380.18.2e-18561.26AMP-dependent synthetase and ligase family protein [more]
AT1G20510.11.9e-12845.31OPC-8:0 CoA ligase1 [more]
AT1G20500.11.1e-12545.93AMP-dependent synthetase and ligase family protein [more]
AT1G20480.11.1e-12345.23AMP-dependent synthetase and ligase family protein [more]
AT5G38120.11.5e-11743.95AMP-dependent synthetase and ligase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Melon (Charmono) v1.1
Date Performed: 2022-10-13
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR042099ANL, N-terminal domainGENE3D3.40.50.12780coord: 34..451
e-value: 8.4E-110
score: 369.4
NoneNo IPR availableGENE3D3.30.300.30coord: 452..552
e-value: 4.2E-29
score: 103.2
NoneNo IPR availablePANTHERPTHR24096:SF2514-COUMARATE--COA LIGASE-LIKE 9coord: 30..547
NoneNo IPR availablePANTHERPTHR24096LONG-CHAIN-FATTY-ACID--COA LIGASEcoord: 30..547
NoneNo IPR availableCDDcd059044CLcoord: 49..546
e-value: 0.0
score: 713.24
NoneNo IPR availableSUPERFAMILY56801Acetyl-CoA synthetase-likecoord: 60..552
IPR025110AMP-binding enzyme, C-terminal domainPFAMPF13193AMP-binding_Ccoord: 465..540
e-value: 9.3E-13
score: 49.0
IPR000873AMP-dependent synthetase/ligasePFAMPF00501AMP-bindingcoord: 61..456
e-value: 5.2E-89
score: 298.8
IPR020845AMP-binding, conserved sitePROSITEPS00455AMP_BINDINGcoord: 202..213

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cmc12g0334251.1Cmc12g0334251.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016020 membrane
molecular_function GO:0016405 CoA-ligase activity