Cmc12g0332491 (gene) Melon (Charmono) v1.1

BLAST of Cmc12g0332491 vs. NCBI nr
Match: XP_008441451.1 (PREDICTED: gibberellin 2-beta-dioxygenase 8 isoform X2 [Cucumis melo])

HSP 1 Score: 705.3 bits (1819), Expect = 2.6e-199
Identity = 344/345 (99.71%), Postives = 345/345 (100.00%), Query Frame = 0

Query: 1   MTHILKQSSANVAMDPPFHEAYKTLLAETAAEAKGTSDVVTVEEWDLPVVDLGRLTGGKV 60
           MTHILKQSSANVAMDPPFHEAYKTLLAETAAEAKGTSDVVTVEEWDLPVVDLGRLTGGKV
Sbjct: 1   MTHILKQSSANVAMDPPFHEAYKTLLAETAAEAKGTSDVVTVEEWDLPVVDLGRLTGGKV 60

Query: 61  EEEEECKNDIIRASKEWGFFQVVNHGISNQLLAKMRAKQIELFKQPFERKSKEDQFSNFS 120
           EEEEECKNDIIRASKEWGFFQVVNHGISNQLLAKMRAKQIELFKQPFERKSKEDQFSNFS
Sbjct: 61  EEEEECKNDIIRASKEWGFFQVVNHGISNQLLAKMRAKQIELFKQPFERKSKEDQFSNFS 120

Query: 121 AGSYRWGTPSATSITQLSWSEAFHVSLSDILGSNYGSDDDLRSTMEEYAGKVSRLAQKLA 180
           AGSYRWGTPSATSITQLSWSEAFHVSLSDILGSNYGSDDDLRSTMEEYAGKVSRLAQKLA
Sbjct: 121 AGSYRWGTPSATSITQLSWSEAFHVSLSDILGSNYGSDDDLRSTMEEYAGKVSRLAQKLA 180

Query: 181 EILGENLGRSSKFFIENCVPSTCYLRMNRYPPCPVQGQIFGLMPHTDSDFLTILHQDQVG 240
           EILGENLGRSSKFF+ENCVPSTCYLRMNRYPPCPVQGQIFGLMPHTDSDFLTILHQDQVG
Sbjct: 181 EILGENLGRSSKFFVENCVPSTCYLRMNRYPPCPVQGQIFGLMPHTDSDFLTILHQDQVG 240

Query: 241 GLELVKDGKWIAVKPNPQALIVNIGDLFQVWSNDEYKSVEHRVVTNSRKERYSIAYFLCP 300
           GLELVKDGKWIAVKPNPQALIVNIGDLFQVWSNDEYKSVEHRVVTNSRKERYSIAYFLCP
Sbjct: 241 GLELVKDGKWIAVKPNPQALIVNIGDLFQVWSNDEYKSVEHRVVTNSRKERYSIAYFLCP 300

Query: 301 WSETVIKSKSEPGVYRRFSFREFRNQVQEDVRKYGYKIGLPRFVL 346
           WSETVIKSKSEPGVYRRFSFREFRNQVQEDVRKYGYKIGLPRFVL
Sbjct: 301 WSETVIKSKSEPGVYRRFSFREFRNQVQEDVRKYGYKIGLPRFVL 345

BLAST of Cmc12g0332491 vs. NCBI nr
Match: XP_008441442.1 (PREDICTED: gibberellin 2-beta-dioxygenase 8 isoform X1 [Cucumis melo])

HSP 1 Score: 694.9 bits (1792), Expect = 3.5e-196
Identity = 339/343 (98.83%), Postives = 342/343 (99.71%), Query Frame = 0

Query: 3   HILKQSSANVAMDPPFHEAYKTLLAETAAEAKGTSDVVTVEEWDLPVVDLGRLTGGKVEE 62
           +I K+SSANVAMDPPFHEAYKTLLAETAAEAKGTSDVVTVEEWDLPVVDLGRLTGGKVEE
Sbjct: 35  NIKKRSSANVAMDPPFHEAYKTLLAETAAEAKGTSDVVTVEEWDLPVVDLGRLTGGKVEE 94

Query: 63  EEECKNDIIRASKEWGFFQVVNHGISNQLLAKMRAKQIELFKQPFERKSKEDQFSNFSAG 122
           EEECKNDIIRASKEWGFFQVVNHGISNQLLAKMRAKQIELFKQPFERKSKEDQFSNFSAG
Sbjct: 95  EEECKNDIIRASKEWGFFQVVNHGISNQLLAKMRAKQIELFKQPFERKSKEDQFSNFSAG 154

Query: 123 SYRWGTPSATSITQLSWSEAFHVSLSDILGSNYGSDDDLRSTMEEYAGKVSRLAQKLAEI 182
           SYRWGTPSATSITQLSWSEAFHVSLSDILGSNYGSDDDLRSTMEEYAGKVSRLAQKLAEI
Sbjct: 155 SYRWGTPSATSITQLSWSEAFHVSLSDILGSNYGSDDDLRSTMEEYAGKVSRLAQKLAEI 214

Query: 183 LGENLGRSSKFFIENCVPSTCYLRMNRYPPCPVQGQIFGLMPHTDSDFLTILHQDQVGGL 242
           LGENLGRSSKFF+ENCVPSTCYLRMNRYPPCPVQGQIFGLMPHTDSDFLTILHQDQVGGL
Sbjct: 215 LGENLGRSSKFFVENCVPSTCYLRMNRYPPCPVQGQIFGLMPHTDSDFLTILHQDQVGGL 274

Query: 243 ELVKDGKWIAVKPNPQALIVNIGDLFQVWSNDEYKSVEHRVVTNSRKERYSIAYFLCPWS 302
           ELVKDGKWIAVKPNPQALIVNIGDLFQVWSNDEYKSVEHRVVTNSRKERYSIAYFLCPWS
Sbjct: 275 ELVKDGKWIAVKPNPQALIVNIGDLFQVWSNDEYKSVEHRVVTNSRKERYSIAYFLCPWS 334

Query: 303 ETVIKSKSEPGVYRRFSFREFRNQVQEDVRKYGYKIGLPRFVL 346
           ETVIKSKSEPGVYRRFSFREFRNQVQEDVRKYGYKIGLPRFVL
Sbjct: 335 ETVIKSKSEPGVYRRFSFREFRNQVQEDVRKYGYKIGLPRFVL 377

BLAST of Cmc12g0332491 vs. NCBI nr
Match: KAA0056882.1 (gibberellin 2-beta-dioxygenase 8 isoform X1 [Cucumis melo var. makuwa] >TYJ99385.1 gibberellin 2-beta-dioxygenase 8 isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 681.4 bits (1757), Expect = 4.0e-192
Identity = 331/332 (99.70%), Postives = 332/332 (100.00%), Query Frame = 0

Query: 14  MDPPFHEAYKTLLAETAAEAKGTSDVVTVEEWDLPVVDLGRLTGGKVEEEEECKNDIIRA 73
           MDPPFHEAYKTLLAETAAEAKGTSDVVTVEEWDLPVVDLGRLTGGKVEEEEECKNDIIRA
Sbjct: 1   MDPPFHEAYKTLLAETAAEAKGTSDVVTVEEWDLPVVDLGRLTGGKVEEEEECKNDIIRA 60

Query: 74  SKEWGFFQVVNHGISNQLLAKMRAKQIELFKQPFERKSKEDQFSNFSAGSYRWGTPSATS 133
           SKEWGFFQVVNHGISNQLLAKMRAKQIELFKQPFERKSKEDQFSNFSAGSYRWGTPSATS
Sbjct: 61  SKEWGFFQVVNHGISNQLLAKMRAKQIELFKQPFERKSKEDQFSNFSAGSYRWGTPSATS 120

Query: 134 ITQLSWSEAFHVSLSDILGSNYGSDDDLRSTMEEYAGKVSRLAQKLAEILGENLGRSSKF 193
           ITQLSWSEAFHVSLSDILGSNYGSDDDLRSTMEEYAGKVSRLAQKLAEILGENLGRSSKF
Sbjct: 121 ITQLSWSEAFHVSLSDILGSNYGSDDDLRSTMEEYAGKVSRLAQKLAEILGENLGRSSKF 180

Query: 194 FIENCVPSTCYLRMNRYPPCPVQGQIFGLMPHTDSDFLTILHQDQVGGLELVKDGKWIAV 253
           F+ENCVPSTCYLRMNRYPPCPVQGQIFGLMPHTDSDFLTILHQDQVGGLELVKDGKWIAV
Sbjct: 181 FVENCVPSTCYLRMNRYPPCPVQGQIFGLMPHTDSDFLTILHQDQVGGLELVKDGKWIAV 240

Query: 254 KPNPQALIVNIGDLFQVWSNDEYKSVEHRVVTNSRKERYSIAYFLCPWSETVIKSKSEPG 313
           KPNPQALIVNIGDLFQVWSNDEYKSVEHRVVTNSRKERYSIAYFLCPWSETVIKSKSEPG
Sbjct: 241 KPNPQALIVNIGDLFQVWSNDEYKSVEHRVVTNSRKERYSIAYFLCPWSETVIKSKSEPG 300

Query: 314 VYRRFSFREFRNQVQEDVRKYGYKIGLPRFVL 346
           VYRRFSFREFRNQVQEDVRKYGYKIGLPRFVL
Sbjct: 301 VYRRFSFREFRNQVQEDVRKYGYKIGLPRFVL 332

BLAST of Cmc12g0332491 vs. NCBI nr
Match: XP_004153456.2 (gibberellin 2-beta-dioxygenase 8 isoform X1 [Cucumis sativus] >XP_031742868.1 gibberellin 2-beta-dioxygenase 8 isoform X1 [Cucumis sativus] >KAE8652673.1 hypothetical protein Csa_013900 [Cucumis sativus])

HSP 1 Score: 651.0 bits (1678), Expect = 5.8e-183
Identity = 325/351 (92.59%), Postives = 331/351 (94.30%), Query Frame = 0

Query: 1   MTHILK-QSSANVAMDPPFHEAYKTLLAETAAE---AKGTSDVV--TVEEWDLPVVDLGR 60
           MTH LK QSSANVAMDPPFHEAYKTLLAE AA    AKG SDVV   VEEWDLP+VDL R
Sbjct: 1   MTHTLKHQSSANVAMDPPFHEAYKTLLAERAAAATGAKGASDVVLTVVEEWDLPLVDLER 60

Query: 61  LTGGKVEEEEECKNDIIRASKEWGFFQVVNHGISNQLLAKMRAKQIELFKQPFERKSKED 120
           LT GKVEE E+CKNDII ASKEWGFFQVVNHGISNQLLAKMRAKQIELFKQPFERKSKED
Sbjct: 61  LTAGKVEEVEQCKNDIITASKEWGFFQVVNHGISNQLLAKMRAKQIELFKQPFERKSKED 120

Query: 121 QFSNFSAGSYRWGTPSATSITQLSWSEAFHVSLSDILGSNYGSDDDLRSTMEEYAGKVSR 180
           QFSNFSAGSYRWGTPSATSITQLSWSEAFHVSLSDILG+N   DDDLRSTMEEYAGKVSR
Sbjct: 121 QFSNFSAGSYRWGTPSATSITQLSWSEAFHVSLSDILGTNGSDDDDLRSTMEEYAGKVSR 180

Query: 181 LAQKLAEILGENLGRSSKFFIENCVPSTCYLRMNRYPPCPVQGQIFGLMPHTDSDFLTIL 240
           LAQKLAEILGENLGRSSKFF+ENCVPSTCYLRMNRYPPC V GQIFGLMPHTDSDFLTIL
Sbjct: 181 LAQKLAEILGENLGRSSKFFVENCVPSTCYLRMNRYPPCHVPGQIFGLMPHTDSDFLTIL 240

Query: 241 HQDQVGGLELVKDGKWIAVKPNPQALIVNIGDLFQVWSNDEYKSVEHRVVTNSRKERYSI 300
           HQDQVGGLELVKDGKWIAVKPNPQALI+NIGDLFQVWSNDEYKSVEHRVVTNS+KERYSI
Sbjct: 241 HQDQVGGLELVKDGKWIAVKPNPQALIINIGDLFQVWSNDEYKSVEHRVVTNSKKERYSI 300

Query: 301 AYFLCPWSETVIKSKSEPGVYRRFSFREFRNQVQEDVRKYGYKIGLPRFVL 346
           AYFLCPWSETVIKSKSEPGVYRRFSFREFRNQVQEDVRKYGYKIGLPRFVL
Sbjct: 301 AYFLCPWSETVIKSKSEPGVYRRFSFREFRNQVQEDVRKYGYKIGLPRFVL 351

BLAST of Cmc12g0332491 vs. NCBI nr
Match: XP_031742874.1 (gibberellin 2-beta-dioxygenase 8 isoform X2 [Cucumis sativus] >CCL98007.1 GA 2-oxidase, partial [Cucumis sativus])

HSP 1 Score: 633.6 bits (1633), Expect = 9.7e-178
Identity = 313/337 (92.88%), Postives = 319/337 (94.66%), Query Frame = 0

Query: 14  MDPPFHEAYKTLLAETAAE---AKGTSDVV--TVEEWDLPVVDLGRLTGGKVEEEEECKN 73
           MDPPFHEAYKTLLAE AA    AKG SDVV   VEEWDLP+VDL RLT GKVEE E+CKN
Sbjct: 1   MDPPFHEAYKTLLAERAAAATGAKGASDVVLTVVEEWDLPLVDLERLTAGKVEEVEQCKN 60

Query: 74  DIIRASKEWGFFQVVNHGISNQLLAKMRAKQIELFKQPFERKSKEDQFSNFSAGSYRWGT 133
           DII ASKEWGFFQVVNHGISNQLLAKMRAKQIELFKQPFERKSKEDQFSNFSAGSYRWGT
Sbjct: 61  DIITASKEWGFFQVVNHGISNQLLAKMRAKQIELFKQPFERKSKEDQFSNFSAGSYRWGT 120

Query: 134 PSATSITQLSWSEAFHVSLSDILGSNYGSDDDLRSTMEEYAGKVSRLAQKLAEILGENLG 193
           PSATSITQLSWSEAFHVSLSDILG+N   DDDLRSTMEEYAGKVSRLAQKLAEILGENLG
Sbjct: 121 PSATSITQLSWSEAFHVSLSDILGTNGSDDDDLRSTMEEYAGKVSRLAQKLAEILGENLG 180

Query: 194 RSSKFFIENCVPSTCYLRMNRYPPCPVQGQIFGLMPHTDSDFLTILHQDQVGGLELVKDG 253
           RSSKFF+ENCVPSTCYLRMNRYPPC V GQIFGLMPHTDSDFLTILHQDQVGGLELVKDG
Sbjct: 181 RSSKFFVENCVPSTCYLRMNRYPPCHVPGQIFGLMPHTDSDFLTILHQDQVGGLELVKDG 240

Query: 254 KWIAVKPNPQALIVNIGDLFQVWSNDEYKSVEHRVVTNSRKERYSIAYFLCPWSETVIKS 313
           KWIAVKPNPQALI+NIGDLFQVWSNDEYKSVEHRVVTNS+KERYSIAYFLCPWSETVIKS
Sbjct: 241 KWIAVKPNPQALIINIGDLFQVWSNDEYKSVEHRVVTNSKKERYSIAYFLCPWSETVIKS 300

Query: 314 KSEPGVYRRFSFREFRNQVQEDVRKYGYKIGLPRFVL 346
           KSEPGVYRRFSFREFRNQVQEDVRKYGYKIGLPRFVL
Sbjct: 301 KSEPGVYRRFSFREFRNQVQEDVRKYGYKIGLPRFVL 337

BLAST of Cmc12g0332491 vs. ExPASy Swiss-Prot
Match: O49561 (Gibberellin 2-beta-dioxygenase 8 OS=Arabidopsis thaliana OX=3702 GN=GA2OX8 PE=1 SV=2)

HSP 1 Score: 421.4 bits (1082), Expect = 9.9e-117
Identity = 205/339 (60.47%), Postives = 253/339 (74.63%), Query Frame = 0

Query: 14  MDPPFHEAYKTLLAETAAEAKGT--------SDVVTVEEWDLPVVDLGRLTGGKVEEEEE 73
           MDPPF+E Y  LL     +            S    VEE +LPV+D+ RL  G  EE E+
Sbjct: 1   MDPPFNEIYNNLLYNQITKKDNDVSEIPFSFSVTAVVEEVELPVIDVSRLIDGAEEEREK 60

Query: 74  CKNDIIRASKEWGFFQVVNHGISNQLLAKMRAKQIELFKQPFERKSKEDQFSNFSAGSYR 133
           CK  I RAS+EWGFFQV+NHGIS  +L KMR +QI +F++PF++KSK ++   FSAGSYR
Sbjct: 61  CKEAIARASREWGFFQVINHGISMDVLEKMRQEQIRVFREPFDKKSKSEK---FSAGSYR 120

Query: 134 WGTPSATSITQLSWSEAFHVSLSDILGSNYGSDDDLRSTMEEYAGKVSRLAQKLAEILGE 193
           WGTPSATSI QLSWSEAFHV ++DI  S+      L STME++A +   LA  LAE+L E
Sbjct: 121 WGTPSATSIRQLSWSEAFHVPMTDI--SDNKDFTTLSSTMEKFASESEALAYMLAEVLAE 180

Query: 194 NLGRSSKFFIENCVPSTCYLRMNRYPPCPVQGQIFGLMPHTDSDFLTILHQDQVGGLELV 253
             G++S FF ENCV +TCYLRMNRYPPCP   +++GLMPHTDSDFLTIL+QDQVGGL+L+
Sbjct: 181 KSGQNSSFFKENCVRNTCYLRMNRYPPCPKPSEVYGLMPHTDSDFLTILYQDQVGGLQLI 240

Query: 254 KDGKWIAVKPNPQALIVNIGDLFQVWSNDEYKSVEHRVVTNSRKERYSIAYFLCPWSETV 313
           KD +WIAVKPNP+ALI+NIGDLFQ WSN  YKSVEHRV+TN + ER+S AYF+CP  + V
Sbjct: 241 KDNRWIAVKPNPKALIINIGDLFQAWSNGMYKSVEHRVMTNPKVERFSTAYFMCPSYDAV 300

Query: 314 IKSKSEPGVYRRFSFREFRNQVQEDVRKYGYKIGLPRFV 345
           I+  S+   YR FSFREFR QVQEDV+K+G+K+GLPRF+
Sbjct: 301 IECSSDRPAYRNFSFREFRQQVQEDVKKFGFKVGLPRFL 334

BLAST of Cmc12g0332491 vs. ExPASy Swiss-Prot
Match: Q7XP65 (Gibberellin 2-beta-dioxygenase 6 OS=Oryza sativa subsp. japonica OX=39947 GN=GA2OX6 PE=1 SV=1)

HSP 1 Score: 342.4 bits (877), Expect = 5.8e-93
Identity = 174/353 (49.29%), Postives = 232/353 (65.72%), Query Frame = 0

Query: 10  ANVAMDPPFHEAYKTL----------LAETAAEAKGTSDVVTVEEWDLPVVDLGRLTGGK 69
           A++A+DPP  ++Y+ L          +A  A +  G+   V   E DLP+VDL RLT G 
Sbjct: 5   ADIAIDPPLADSYRALALLRRDRDGGIAPPAVQMVGSGGAVL--ERDLPMVDLERLTRGG 64

Query: 70  VEEEEECKNDIIRASKEWGFFQVVNHGISNQLLAKMRAKQIELFKQPFERKSKEDQFSNF 129
             E + C   + RA+ EWGFFQ+ NHG+  +L+ +MR +Q  LF+ PFE K K    +  
Sbjct: 65  AGERKACAGAMARAASEWGFFQLTNHGVGRELMEEMRREQARLFRLPFETKEK----AGL 124

Query: 130 SAGSYRWGTPSATSITQLSWSEAFHVSLSDILGS--NYGSDDDLRSTMEEYAGKVSRLAQ 189
             GSYRWG P+ATS+  LSWSEAFHV L+ I G+  ++G    LR  M+E A  +SR+A 
Sbjct: 125 LNGSYRWGNPTATSLRHLSWSEAFHVPLASISGADCDFGDLTSLRGVMQEVAEAMSRVAN 184

Query: 190 KLAEILGENL-GR------SSKFFIENCVPSTCYLRMNRYPPCPVQGQIFGLMPHTDSDF 249
            +A  L E L GR      ++ +F   C  +TC+LR+NRYP CP     FGL+PHTDSDF
Sbjct: 185 TVAAALAEELTGRGGGGASAAPWFPAGCDETTCFLRLNRYPACPFAADTFGLVPHTDSDF 244

Query: 250 LTILHQDQVGGLELVKDGKWIAVKPNPQALIVNIGDLFQVWSNDEYKSVEHRVVTNSRKE 309
           LT+L QDQVGGL L+KD +W+AV+P P AL+VNIGDLFQ WSN+ YKSVEH+VV N++ +
Sbjct: 245 LTVLCQDQVGGLHLMKDSRWVAVRPRPDALVVNIGDLFQAWSNNRYKSVEHKVVANAKTD 304

Query: 310 RYSIAYFLCPWSETVIKSKSEPGVYRRFSFREFRNQVQEDVRKYGYKIGLPRF 344
           R S+AYFLCP  ++++ +  EP  YR F+F E+R +VQEDVR  G KIGLP F
Sbjct: 305 RLSVAYFLCPSYDSLVGTCGEPSPYRAFTFGEYRKKVQEDVRTTGKKIGLPNF 351

BLAST of Cmc12g0332491 vs. ExPASy Swiss-Prot
Match: Q8LGZ9 (Gibberellin 2-beta-dioxygenase 5 OS=Oryza sativa subsp. japonica OX=39947 GN=GA2OX5 PE=1 SV=1)

HSP 1 Score: 295.8 bits (756), Expect = 6.3e-79
Identity = 159/337 (47.18%), Postives = 211/337 (62.61%), Query Frame = 0

Query: 15  DPPFHEAYKTLLAETAAEAKGTSDVVTVEEWDLPVVDLGRLTGGKVEEEEECKNDIIRAS 74
           +PP    YK L  E           + V+E +LPV+DL     G +E E+ C+ D++RA+
Sbjct: 11  NPPLMSTYKHLFVE-QHRLDMDMGAIDVDECELPVIDL----AGLMEAEQVCRADMVRAA 70

Query: 75  KEWGFFQVVNHGISNQLLAKMRAKQIELFKQPFERKSKEDQFSNFSAGSYRWGTPSATSI 134
            EWGFFQV NHG+   LL ++   Q+ +F++PF+ K  E +   FS  SYRWGTP+A  +
Sbjct: 71  SEWGFFQVTNHGVPQALLRELHDAQVAVFRRPFQEKVTE-RLLGFSPESYRWGTPTAKCL 130

Query: 135 TQLSWSEAFHVSLSDILGSNYGSDDDLRSTMEEYAGKVSRLAQKLAEILGENL-GRSSKF 194
            QLSWSEA+H+ ++    S   +    R+ +EE +  +  LAQKLAEIL   L G     
Sbjct: 131 EQLSWSEAYHIPMTTPRPS---TSIRARAVIEEVSRAMYELAQKLAEILMRGLPGAGEGE 190

Query: 195 FIENCVPSTCYLRMNRYPPCPVQGQIFGLMPHTDSDFLTILH--QDQVGGLELVKDGKWI 254
            +      TC+LR+NRYPPC +    FGL PHTDSD LTI+H  QD VGGL+L+K G+W+
Sbjct: 191 TMVTTREETCFLRLNRYPPCAMAMGGFGLCPHTDSDLLTIVHQQQDTVGGLQLLKGGRWV 250

Query: 255 AVKPNPQALIVNIGDLFQVWSNDEYKSVEHRVVTNSRKERYSIAYFLCPWSETVIKSKS- 314
           AVKP+P  LIVN+GDL Q WSND YKSVEHRV+ N+  ER+S+A+FLCP   T+I   S 
Sbjct: 251 AVKPSPSTLIVNVGDLLQAWSNDVYKSVEHRVMANATLERFSMAFFLCPSYHTLIIPSSS 310

Query: 315 ----EPGVYRRFSFREFRNQVQEDVRKYGYKIGLPRF 344
               +   YR F+F E+R Q+ EDVR  G KIGL RF
Sbjct: 311 HVHDDDAHYRSFTFGEYRKQIMEDVRSTGRKIGLHRF 338

BLAST of Cmc12g0332491 vs. ExPASy Swiss-Prot
Match: Q9C6I4 (Gibberellin 2-beta-dioxygenase 7 OS=Arabidopsis thaliana OX=3702 GN=GA2OX7 PE=1 SV=1)

HSP 1 Score: 293.5 bits (750), Expect = 3.1e-78
Identity = 150/339 (44.25%), Postives = 218/339 (64.31%), Query Frame = 0

Query: 12  VAMDPPFHEAYKTLLA----ETAAEAKGTSDVVTVEEWDLPVVDLGRLTGGKVEEEEECK 71
           +A  PPF   + ++       + +E+   +  +      LPV+DL  LT G+  + + C 
Sbjct: 1   MASQPPFKTNFCSIFGSSFPNSTSESNTNTSTIQTSGIKLPVIDLSHLTSGEEVKRKRCV 60

Query: 72  NDIIRASKEWGFFQVVNHGISNQLLAKMRAKQIELFKQPFERKSKEDQFSNFSAGSYRWG 131
             ++ A+KEWGFFQ+VNHGI   +   M  ++ +LF QPF  K +E +FS+ S  SYRWG
Sbjct: 61  KQMVAAAKEWGFFQIVNHGIPKDVFEMMLLEEKKLFDQPFSVKVRE-RFSDLSKNSYRWG 120

Query: 132 TPSATSITQLSWSEAFHVSLSDILGSNYGSD-DDLRSTMEEYAGKVSRLAQKLAEILGEN 191
            PSATS  Q S SEAFH+ LS++  S    D ++LR+ +E Y  +++R+AQ + EILG+ 
Sbjct: 121 NPSATSPAQYSVSEAFHIILSEV--SRISDDRNNLRTIVETYVQEIARVAQMICEILGKQ 180

Query: 192 LGRSSKFFIENCVPSTCYLRMNRYPPCPVQGQIFGLMPHTDSDFLTILHQDQVGGLELVK 251
           +  SS++F         +LR+N+Y P     ++FGL+PHTD+ FLTIL QDQ+GGLEL  
Sbjct: 181 VNVSSEYFENIFELENSFLRLNKYHPSVFGSEVFGLVPHTDTSFLTILSQDQIGGLELEN 240

Query: 252 DGKWIAVKPNPQALIVNIGDLFQVWSNDEYKSVEHRVVTNSRKERYSIAYFLCPWSETVI 311
           +G+WI+VKP  +AL VNIGD+FQ  SN  Y+SV HRV++ +  ER SIA+F+CP+ ET I
Sbjct: 241 NGQWISVKPCLEALTVNIGDMFQALSNGVYQSVRHRVISPANIERMSIAFFVCPYLETEI 300

Query: 312 KSKSEPGVYRRFSFREFRNQVQEDVRKYGYKIGLPRFVL 346
                P  YRRFSFRE++ Q + DV++ G K+GL RF++
Sbjct: 301 DCFGYPKKYRRFSFREYKEQSEHDVKETGDKVGLSRFLI 336

BLAST of Cmc12g0332491 vs. ExPASy Swiss-Prot
Match: Q39112 (Gibberellin 20 oxidase 3 OS=Arabidopsis thaliana OX=3702 GN=GA20OX3 PE=2 SV=1)

HSP 1 Score: 167.5 bits (423), Expect = 2.6e-40
Identity = 99/289 (34.26%), Postives = 138/289 (47.75%), Query Frame = 0

Query: 42  VEEWDLPVVDLGRLTGGKVEEEEECKNDIIRASKEWGFFQVVNHGISNQLLAKMRAKQIE 101
           V+   +P++DL     G      E    + +A+ + GFF + NHG+   LL++       
Sbjct: 53  VQPLQVPLIDLAGFLSGDSCLASEATRLVSKAATKHGFFLITNHGVDESLLSRAYLHMDS 112

Query: 102 LFKQPFERKSKEDQFSNFSAGSYRWGTPS--ATSI-----TQLSWSEAFHVSLS------ 161
            FK P   K K  +         +WG  S  A+S      ++L W E      S      
Sbjct: 113 FFKAPACEKQKAQR---------KWGESSGYASSFVGRFSSKLPWKETLSFKFSPEEKIH 172

Query: 162 -----DILGSNYGSD-DDLRSTMEEYAGKVSRLAQKLAEILGENLGRSSKFFIENCVPST 221
                D +    G   +D     +EYA  ++ L+ K+ E+LG +LG   ++F E    S 
Sbjct: 173 SQTVKDFVSKKMGDGYEDFGKVYQEYAEAMNTLSLKIMELLGMSLGVERRYFKEFFEDSD 232

Query: 222 CYLRMNRYPPCPVQGQIFGLMPHTDSDFLTILHQDQVGGLELVKDGKWIAVKPNPQALIV 281
              R+N YP C       G  PH D   LTILHQDQVGGL++  D KW ++ PNP A +V
Sbjct: 233 SIFRLNYYPQCKQPELALGTGPHCDPTSLTILHQDQVGGLQVFVDNKWQSIPPNPHAFVV 292

Query: 282 NIGDLFQVWSNDEYKSVEHRVVTNSRKERYSIAYFLCPWSETVIKSKSE 312
           NIGD F   +N  YKS  HR V NS +ER + A+FLCP  E V+K   E
Sbjct: 293 NIGDTFMALTNGRYKSCLHRAVVNSERERKTFAFFLCPKGEKVVKPPEE 332

BLAST of Cmc12g0332491 vs. ExPASy TrEMBL
Match: A0A1S3B309 (gibberellin 2-beta-dioxygenase 8 isoform X2 OS=Cucumis melo OX=3656 GN=LOC103485556 PE=3 SV=1)

HSP 1 Score: 705.3 bits (1819), Expect = 1.3e-199
Identity = 344/345 (99.71%), Postives = 345/345 (100.00%), Query Frame = 0

Query: 1   MTHILKQSSANVAMDPPFHEAYKTLLAETAAEAKGTSDVVTVEEWDLPVVDLGRLTGGKV 60
           MTHILKQSSANVAMDPPFHEAYKTLLAETAAEAKGTSDVVTVEEWDLPVVDLGRLTGGKV
Sbjct: 1   MTHILKQSSANVAMDPPFHEAYKTLLAETAAEAKGTSDVVTVEEWDLPVVDLGRLTGGKV 60

Query: 61  EEEEECKNDIIRASKEWGFFQVVNHGISNQLLAKMRAKQIELFKQPFERKSKEDQFSNFS 120
           EEEEECKNDIIRASKEWGFFQVVNHGISNQLLAKMRAKQIELFKQPFERKSKEDQFSNFS
Sbjct: 61  EEEEECKNDIIRASKEWGFFQVVNHGISNQLLAKMRAKQIELFKQPFERKSKEDQFSNFS 120

Query: 121 AGSYRWGTPSATSITQLSWSEAFHVSLSDILGSNYGSDDDLRSTMEEYAGKVSRLAQKLA 180
           AGSYRWGTPSATSITQLSWSEAFHVSLSDILGSNYGSDDDLRSTMEEYAGKVSRLAQKLA
Sbjct: 121 AGSYRWGTPSATSITQLSWSEAFHVSLSDILGSNYGSDDDLRSTMEEYAGKVSRLAQKLA 180

Query: 181 EILGENLGRSSKFFIENCVPSTCYLRMNRYPPCPVQGQIFGLMPHTDSDFLTILHQDQVG 240
           EILGENLGRSSKFF+ENCVPSTCYLRMNRYPPCPVQGQIFGLMPHTDSDFLTILHQDQVG
Sbjct: 181 EILGENLGRSSKFFVENCVPSTCYLRMNRYPPCPVQGQIFGLMPHTDSDFLTILHQDQVG 240

Query: 241 GLELVKDGKWIAVKPNPQALIVNIGDLFQVWSNDEYKSVEHRVVTNSRKERYSIAYFLCP 300
           GLELVKDGKWIAVKPNPQALIVNIGDLFQVWSNDEYKSVEHRVVTNSRKERYSIAYFLCP
Sbjct: 241 GLELVKDGKWIAVKPNPQALIVNIGDLFQVWSNDEYKSVEHRVVTNSRKERYSIAYFLCP 300

Query: 301 WSETVIKSKSEPGVYRRFSFREFRNQVQEDVRKYGYKIGLPRFVL 346
           WSETVIKSKSEPGVYRRFSFREFRNQVQEDVRKYGYKIGLPRFVL
Sbjct: 301 WSETVIKSKSEPGVYRRFSFREFRNQVQEDVRKYGYKIGLPRFVL 345

BLAST of Cmc12g0332491 vs. ExPASy TrEMBL
Match: A0A1S3B3F3 (gibberellin 2-beta-dioxygenase 8 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103485556 PE=3 SV=1)

HSP 1 Score: 694.9 bits (1792), Expect = 1.7e-196
Identity = 339/343 (98.83%), Postives = 342/343 (99.71%), Query Frame = 0

Query: 3   HILKQSSANVAMDPPFHEAYKTLLAETAAEAKGTSDVVTVEEWDLPVVDLGRLTGGKVEE 62
           +I K+SSANVAMDPPFHEAYKTLLAETAAEAKGTSDVVTVEEWDLPVVDLGRLTGGKVEE
Sbjct: 35  NIKKRSSANVAMDPPFHEAYKTLLAETAAEAKGTSDVVTVEEWDLPVVDLGRLTGGKVEE 94

Query: 63  EEECKNDIIRASKEWGFFQVVNHGISNQLLAKMRAKQIELFKQPFERKSKEDQFSNFSAG 122
           EEECKNDIIRASKEWGFFQVVNHGISNQLLAKMRAKQIELFKQPFERKSKEDQFSNFSAG
Sbjct: 95  EEECKNDIIRASKEWGFFQVVNHGISNQLLAKMRAKQIELFKQPFERKSKEDQFSNFSAG 154

Query: 123 SYRWGTPSATSITQLSWSEAFHVSLSDILGSNYGSDDDLRSTMEEYAGKVSRLAQKLAEI 182
           SYRWGTPSATSITQLSWSEAFHVSLSDILGSNYGSDDDLRSTMEEYAGKVSRLAQKLAEI
Sbjct: 155 SYRWGTPSATSITQLSWSEAFHVSLSDILGSNYGSDDDLRSTMEEYAGKVSRLAQKLAEI 214

Query: 183 LGENLGRSSKFFIENCVPSTCYLRMNRYPPCPVQGQIFGLMPHTDSDFLTILHQDQVGGL 242
           LGENLGRSSKFF+ENCVPSTCYLRMNRYPPCPVQGQIFGLMPHTDSDFLTILHQDQVGGL
Sbjct: 215 LGENLGRSSKFFVENCVPSTCYLRMNRYPPCPVQGQIFGLMPHTDSDFLTILHQDQVGGL 274

Query: 243 ELVKDGKWIAVKPNPQALIVNIGDLFQVWSNDEYKSVEHRVVTNSRKERYSIAYFLCPWS 302
           ELVKDGKWIAVKPNPQALIVNIGDLFQVWSNDEYKSVEHRVVTNSRKERYSIAYFLCPWS
Sbjct: 275 ELVKDGKWIAVKPNPQALIVNIGDLFQVWSNDEYKSVEHRVVTNSRKERYSIAYFLCPWS 334

Query: 303 ETVIKSKSEPGVYRRFSFREFRNQVQEDVRKYGYKIGLPRFVL 346
           ETVIKSKSEPGVYRRFSFREFRNQVQEDVRKYGYKIGLPRFVL
Sbjct: 335 ETVIKSKSEPGVYRRFSFREFRNQVQEDVRKYGYKIGLPRFVL 377

BLAST of Cmc12g0332491 vs. ExPASy TrEMBL
Match: A0A5A7UM12 (Gibberellin 2-beta-dioxygenase 8 isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G006030 PE=3 SV=1)

HSP 1 Score: 681.4 bits (1757), Expect = 2.0e-192
Identity = 331/332 (99.70%), Postives = 332/332 (100.00%), Query Frame = 0

Query: 14  MDPPFHEAYKTLLAETAAEAKGTSDVVTVEEWDLPVVDLGRLTGGKVEEEEECKNDIIRA 73
           MDPPFHEAYKTLLAETAAEAKGTSDVVTVEEWDLPVVDLGRLTGGKVEEEEECKNDIIRA
Sbjct: 1   MDPPFHEAYKTLLAETAAEAKGTSDVVTVEEWDLPVVDLGRLTGGKVEEEEECKNDIIRA 60

Query: 74  SKEWGFFQVVNHGISNQLLAKMRAKQIELFKQPFERKSKEDQFSNFSAGSYRWGTPSATS 133
           SKEWGFFQVVNHGISNQLLAKMRAKQIELFKQPFERKSKEDQFSNFSAGSYRWGTPSATS
Sbjct: 61  SKEWGFFQVVNHGISNQLLAKMRAKQIELFKQPFERKSKEDQFSNFSAGSYRWGTPSATS 120

Query: 134 ITQLSWSEAFHVSLSDILGSNYGSDDDLRSTMEEYAGKVSRLAQKLAEILGENLGRSSKF 193
           ITQLSWSEAFHVSLSDILGSNYGSDDDLRSTMEEYAGKVSRLAQKLAEILGENLGRSSKF
Sbjct: 121 ITQLSWSEAFHVSLSDILGSNYGSDDDLRSTMEEYAGKVSRLAQKLAEILGENLGRSSKF 180

Query: 194 FIENCVPSTCYLRMNRYPPCPVQGQIFGLMPHTDSDFLTILHQDQVGGLELVKDGKWIAV 253
           F+ENCVPSTCYLRMNRYPPCPVQGQIFGLMPHTDSDFLTILHQDQVGGLELVKDGKWIAV
Sbjct: 181 FVENCVPSTCYLRMNRYPPCPVQGQIFGLMPHTDSDFLTILHQDQVGGLELVKDGKWIAV 240

Query: 254 KPNPQALIVNIGDLFQVWSNDEYKSVEHRVVTNSRKERYSIAYFLCPWSETVIKSKSEPG 313
           KPNPQALIVNIGDLFQVWSNDEYKSVEHRVVTNSRKERYSIAYFLCPWSETVIKSKSEPG
Sbjct: 241 KPNPQALIVNIGDLFQVWSNDEYKSVEHRVVTNSRKERYSIAYFLCPWSETVIKSKSEPG 300

Query: 314 VYRRFSFREFRNQVQEDVRKYGYKIGLPRFVL 346
           VYRRFSFREFRNQVQEDVRKYGYKIGLPRFVL
Sbjct: 301 VYRRFSFREFRNQVQEDVRKYGYKIGLPRFVL 332

BLAST of Cmc12g0332491 vs. ExPASy TrEMBL
Match: S0CRC1 (GA 2-oxidase (Fragment) OS=Cucumis sativus OX=3659 GN=GA2ox5 PE=2 SV=1)

HSP 1 Score: 633.6 bits (1633), Expect = 4.7e-178
Identity = 313/337 (92.88%), Postives = 319/337 (94.66%), Query Frame = 0

Query: 14  MDPPFHEAYKTLLAETAAE---AKGTSDVV--TVEEWDLPVVDLGRLTGGKVEEEEECKN 73
           MDPPFHEAYKTLLAE AA    AKG SDVV   VEEWDLP+VDL RLT GKVEE E+CKN
Sbjct: 1   MDPPFHEAYKTLLAERAAAATGAKGASDVVLTVVEEWDLPLVDLERLTAGKVEEVEQCKN 60

Query: 74  DIIRASKEWGFFQVVNHGISNQLLAKMRAKQIELFKQPFERKSKEDQFSNFSAGSYRWGT 133
           DII ASKEWGFFQVVNHGISNQLLAKMRAKQIELFKQPFERKSKEDQFSNFSAGSYRWGT
Sbjct: 61  DIITASKEWGFFQVVNHGISNQLLAKMRAKQIELFKQPFERKSKEDQFSNFSAGSYRWGT 120

Query: 134 PSATSITQLSWSEAFHVSLSDILGSNYGSDDDLRSTMEEYAGKVSRLAQKLAEILGENLG 193
           PSATSITQLSWSEAFHVSLSDILG+N   DDDLRSTMEEYAGKVSRLAQKLAEILGENLG
Sbjct: 121 PSATSITQLSWSEAFHVSLSDILGTNGSDDDDLRSTMEEYAGKVSRLAQKLAEILGENLG 180

Query: 194 RSSKFFIENCVPSTCYLRMNRYPPCPVQGQIFGLMPHTDSDFLTILHQDQVGGLELVKDG 253
           RSSKFF+ENCVPSTCYLRMNRYPPC V GQIFGLMPHTDSDFLTILHQDQVGGLELVKDG
Sbjct: 181 RSSKFFVENCVPSTCYLRMNRYPPCHVPGQIFGLMPHTDSDFLTILHQDQVGGLELVKDG 240

Query: 254 KWIAVKPNPQALIVNIGDLFQVWSNDEYKSVEHRVVTNSRKERYSIAYFLCPWSETVIKS 313
           KWIAVKPNPQALI+NIGDLFQVWSNDEYKSVEHRVVTNS+KERYSIAYFLCPWSETVIKS
Sbjct: 241 KWIAVKPNPQALIINIGDLFQVWSNDEYKSVEHRVVTNSKKERYSIAYFLCPWSETVIKS 300

Query: 314 KSEPGVYRRFSFREFRNQVQEDVRKYGYKIGLPRFVL 346
           KSEPGVYRRFSFREFRNQVQEDVRKYGYKIGLPRFVL
Sbjct: 301 KSEPGVYRRFSFREFRNQVQEDVRKYGYKIGLPRFVL 337

BLAST of Cmc12g0332491 vs. ExPASy TrEMBL
Match: A0A6J1F422 (gibberellin 2-beta-dioxygenase 8 OS=Cucurbita moschata OX=3662 GN=LOC111440045 PE=3 SV=1)

HSP 1 Score: 587.4 bits (1513), Expect = 3.8e-164
Identity = 289/346 (83.53%), Postives = 314/346 (90.75%), Query Frame = 0

Query: 1   MTHILKQSSANVAMDPPFHEAYKTLLAETAAEAKGTSDVVTVEEWDLPVVDLGRL-TGGK 60
           M H L Q SANVAMDPPFHEAYK+LLAETA E  G  D V V+EW+LPVVDL RL  G +
Sbjct: 1   MIHTLNQ-SANVAMDPPFHEAYKSLLAETAVERGG--DAVAVKEWELPVVDLRRLRDGAE 60

Query: 61  VEEEEECKNDIIRASKEWGFFQVVNHGISNQLLAKMRAKQIELFKQPFERKSKEDQFSNF 120
           V E E+CKNDIIRAS+EWGFFQVVNHG+S+QLL KMRAKQI LFKQPFERKSKED F NF
Sbjct: 61  VGEVEQCKNDIIRASQEWGFFQVVNHGVSSQLLGKMRAKQIGLFKQPFERKSKEDMFLNF 120

Query: 121 SAGSYRWGTPSATSITQLSWSEAFHVSLSDILGSNYGSDDDLRSTMEEYAGKVSRLAQKL 180
           SAGSYRWGTP+ATS+TQLSWSEAFHVSLSDILGSN    DDLRSTMEE+AGKVSRLAQ+L
Sbjct: 121 SAGSYRWGTPTATSLTQLSWSEAFHVSLSDILGSN--GSDDLRSTMEEFAGKVSRLAQEL 180

Query: 181 AEILGENLGRSSKFFIENCVPSTCYLRMNRYPPCPVQGQIFGLMPHTDSDFLTILHQDQV 240
           AEILGENLGRSSKFF++NCVPSTCYLRMNRYPPC V G++FGLMPHTDSDFLTILHQDQV
Sbjct: 181 AEILGENLGRSSKFFVDNCVPSTCYLRMNRYPPCSVPGRVFGLMPHTDSDFLTILHQDQV 240

Query: 241 GGLELVKDGKWIAVKPNPQALIVNIGDLFQVWSNDEYKSVEHRVVTNSRKERYSIAYFLC 300
           GGLELVKDG+WIAVKPNP+ALI+NIGDLFQ WSN+ YKSVEHRVVTNS+ ER+SIAYF C
Sbjct: 241 GGLELVKDGEWIAVKPNPEALIINIGDLFQAWSNNVYKSVEHRVVTNSKLERFSIAYFFC 300

Query: 301 PWSETVIKSKSEPGVYRRFSFREFRNQVQEDVRKYGYKIGLPRFVL 346
           PWSETVIKSK EPGVYRRFSFREFRNQVQEDVRK+G+KIGLP+FVL
Sbjct: 301 PWSETVIKSKCEPGVYRRFSFREFRNQVQEDVRKHGFKIGLPKFVL 341

BLAST of Cmc12g0332491 vs. TAIR 10
Match: AT4G21200.1 (gibberellin 2-oxidase 8 )

HSP 1 Score: 421.4 bits (1082), Expect = 7.0e-118
Identity = 205/339 (60.47%), Postives = 253/339 (74.63%), Query Frame = 0

Query: 14  MDPPFHEAYKTLLAETAAEAKGT--------SDVVTVEEWDLPVVDLGRLTGGKVEEEEE 73
           MDPPF+E Y  LL     +            S    VEE +LPV+D+ RL  G  EE E+
Sbjct: 1   MDPPFNEIYNNLLYNQITKKDNDVSEIPFSFSVTAVVEEVELPVIDVSRLIDGAEEEREK 60

Query: 74  CKNDIIRASKEWGFFQVVNHGISNQLLAKMRAKQIELFKQPFERKSKEDQFSNFSAGSYR 133
           CK  I RAS+EWGFFQV+NHGIS  +L KMR +QI +F++PF++KSK ++   FSAGSYR
Sbjct: 61  CKEAIARASREWGFFQVINHGISMDVLEKMRQEQIRVFREPFDKKSKSEK---FSAGSYR 120

Query: 134 WGTPSATSITQLSWSEAFHVSLSDILGSNYGSDDDLRSTMEEYAGKVSRLAQKLAEILGE 193
           WGTPSATSI QLSWSEAFHV ++DI  S+      L STME++A +   LA  LAE+L E
Sbjct: 121 WGTPSATSIRQLSWSEAFHVPMTDI--SDNKDFTTLSSTMEKFASESEALAYMLAEVLAE 180

Query: 194 NLGRSSKFFIENCVPSTCYLRMNRYPPCPVQGQIFGLMPHTDSDFLTILHQDQVGGLELV 253
             G++S FF ENCV +TCYLRMNRYPPCP   +++GLMPHTDSDFLTIL+QDQVGGL+L+
Sbjct: 181 KSGQNSSFFKENCVRNTCYLRMNRYPPCPKPSEVYGLMPHTDSDFLTILYQDQVGGLQLI 240

Query: 254 KDGKWIAVKPNPQALIVNIGDLFQVWSNDEYKSVEHRVVTNSRKERYSIAYFLCPWSETV 313
           KD +WIAVKPNP+ALI+NIGDLFQ WSN  YKSVEHRV+TN + ER+S AYF+CP  + V
Sbjct: 241 KDNRWIAVKPNPKALIINIGDLFQAWSNGMYKSVEHRVMTNPKVERFSTAYFMCPSYDAV 300

Query: 314 IKSKSEPGVYRRFSFREFRNQVQEDVRKYGYKIGLPRFV 345
           I+  S+   YR FSFREFR QVQEDV+K+G+K+GLPRF+
Sbjct: 301 IECSSDRPAYRNFSFREFRQQVQEDVKKFGFKVGLPRFL 334

BLAST of Cmc12g0332491 vs. TAIR 10
Match: AT1G50960.1 (gibberellin 2-oxidase 7 )

HSP 1 Score: 293.5 bits (750), Expect = 2.2e-79
Identity = 150/339 (44.25%), Postives = 218/339 (64.31%), Query Frame = 0

Query: 12  VAMDPPFHEAYKTLLA----ETAAEAKGTSDVVTVEEWDLPVVDLGRLTGGKVEEEEECK 71
           +A  PPF   + ++       + +E+   +  +      LPV+DL  LT G+  + + C 
Sbjct: 1   MASQPPFKTNFCSIFGSSFPNSTSESNTNTSTIQTSGIKLPVIDLSHLTSGEEVKRKRCV 60

Query: 72  NDIIRASKEWGFFQVVNHGISNQLLAKMRAKQIELFKQPFERKSKEDQFSNFSAGSYRWG 131
             ++ A+KEWGFFQ+VNHGI   +   M  ++ +LF QPF  K +E +FS+ S  SYRWG
Sbjct: 61  KQMVAAAKEWGFFQIVNHGIPKDVFEMMLLEEKKLFDQPFSVKVRE-RFSDLSKNSYRWG 120

Query: 132 TPSATSITQLSWSEAFHVSLSDILGSNYGSD-DDLRSTMEEYAGKVSRLAQKLAEILGEN 191
            PSATS  Q S SEAFH+ LS++  S    D ++LR+ +E Y  +++R+AQ + EILG+ 
Sbjct: 121 NPSATSPAQYSVSEAFHIILSEV--SRISDDRNNLRTIVETYVQEIARVAQMICEILGKQ 180

Query: 192 LGRSSKFFIENCVPSTCYLRMNRYPPCPVQGQIFGLMPHTDSDFLTILHQDQVGGLELVK 251
           +  SS++F         +LR+N+Y P     ++FGL+PHTD+ FLTIL QDQ+GGLEL  
Sbjct: 181 VNVSSEYFENIFELENSFLRLNKYHPSVFGSEVFGLVPHTDTSFLTILSQDQIGGLELEN 240

Query: 252 DGKWIAVKPNPQALIVNIGDLFQVWSNDEYKSVEHRVVTNSRKERYSIAYFLCPWSETVI 311
           +G+WI+VKP  +AL VNIGD+FQ  SN  Y+SV HRV++ +  ER SIA+F+CP+ ET I
Sbjct: 241 NGQWISVKPCLEALTVNIGDMFQALSNGVYQSVRHRVISPANIERMSIAFFVCPYLETEI 300

Query: 312 KSKSEPGVYRRFSFREFRNQVQEDVRKYGYKIGLPRFVL 346
                P  YRRFSFRE++ Q + DV++ G K+GL RF++
Sbjct: 301 DCFGYPKKYRRFSFREYKEQSEHDVKETGDKVGLSRFLI 336

BLAST of Cmc12g0332491 vs. TAIR 10
Match: AT5G58660.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 182.6 bits (462), Expect = 5.5e-46
Identity = 123/337 (36.50%), Postives = 178/337 (52.82%), Query Frame = 0

Query: 36  TSDVVTVEEWDLPVVDLGRLTGGKVEEEEECKNDIIRASKEWGFFQVVNHGISNQLLAKM 95
           T  +V  ++ ++PV+DL RL           K  +  A KEWG F++ NHG+   L +++
Sbjct: 23  TPVIVQGKDINIPVIDLERLD----------KEILREACKEWGIFRLENHGVPLALTSRL 82

Query: 96  RAKQIELFKQPFERKSKEDQFSNFSAGSYRWGTPS----------ATSITQLSWSEAFHV 155
           +     L   PFE+K +E   +  S  SY WGTP+              + L+  E F+V
Sbjct: 83  QEISESLLSLPFEKK-RELFAAVKSPLSYFWGTPALNRSGDALKRGAQASNLTMLEGFNV 142

Query: 156 SLSDI-----LGSNYGSDDD---------LRSTMEEYAGKVSRLAQKLAEILGE--NLGR 215
            LS +     L ++   DDD          R  MEEY   ++R+A  L E + +  NL  
Sbjct: 143 PLSSLSSLSKLPTSTCCDDDAQEEPKLESFRVLMEEYGKHITRIAVSLFEAIAQTLNLEL 202

Query: 216 SSKFFIENCVPSTCYLRMNRYPPCPVQG--QIFGLMPHTDSDFLTILHQDQVGGLELVKD 275
           S     E    ST  +R+ RYP    +   +  G+  HTDS  ++IL +D+ GGLE++K 
Sbjct: 203 SGNRRSEYLSESTGLIRVYRYPQSSEEAAREALGMEVHTDSSVISILREDESGGLEIMKG 262

Query: 276 GKWIAVKPNPQALIVNIGDLFQVWSNDEYKSVEHRVVTNSRK-ERYSIAYFLCPWSETVI 335
            +W  VKP    LIVN+GD+ Q  S+DEYKSV HRV   +RK ER+S+ YF+ P  + VI
Sbjct: 263 EEWFCVKPVANTLIVNLGDMMQAISDDEYKSVTHRVKKRNRKTERHSVCYFVFPKRDCVI 322

Query: 336 KSKSEPGVYRRFSFREFRNQVQEDVRKYGYKIGLPRF 344
           KS +    Y+ F++ +F  QVQ DV+  G KIGLPRF
Sbjct: 323 KSSN----YKLFTYSDFEAQVQADVQSLGTKIGLPRF 344

BLAST of Cmc12g0332491 vs. TAIR 10
Match: AT3G19010.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 167.5 bits (423), Expect = 1.8e-41
Identity = 103/330 (31.21%), Postives = 166/330 (50.30%), Query Frame = 0

Query: 30  AAEAKGTSDVVTVEEWDLPVVDLGRLTGGKVEEEEECKNDIIRASKEWGFFQVVNHGISN 89
           A E +  S+ +  +  ++PV+DL RL     E+ +   ++I  A ++WGFFQV+NHG+ +
Sbjct: 11  APEHRSDSNFIPNQPEEIPVIDLSRL--DDPEDVQNVISEIGDACEKWGFFQVLNHGVPS 70

Query: 90  QLLAKMRAKQIELFKQPFERKSKEDQFSNFSAGSYRWGTPSATSITQLSWSEAFHVSLSD 149
               ++       F  P E K K  +  + +   Y  G  +        W E F +   D
Sbjct: 71  DARQRVEKTVKMFFDLPMEEKIKVKR-DDVNPVGYHDGEHTK---NVKDWKEVFDIYFKD 130

Query: 150 --ILGSNYGSDD---------------DLRSTMEEYAGKVSRLAQKLAEILGENLGRSSK 209
             ++ S    +D               D R   E YA    +LA KL E++  +LG   +
Sbjct: 131 PMVIPSTTDPEDEGLRLVYNKWPQSPSDFREACEVYARHAEKLAFKLLELISLSLGLPKE 190

Query: 210 FFIENCVPSTCYLRMNRYPPCPVQGQIFGLMPHTDSDFLTILHQDQVGGLELVK--DGKW 269
            F +       + R+NRYPPCP      G+  H D+D +++L QD VGGL++ +  DG W
Sbjct: 191 RFHDYFKEQMSFFRINRYPPCPRPDLALGVGHHKDADVISLLAQDDVGGLQVSRRSDGVW 250

Query: 270 IAVKPNPQALIVNIGDLFQVWSNDEYKSVEHRVVTNSRKERYSIAYFLCPWSETVIK--- 329
             ++P P AL++NIG+  ++W+ND+Y S EHRVV N+ +ERYSI +FL P  +  +K   
Sbjct: 251 FPIRPVPNALVINIGNCMEIWTNDKYWSAEHRVVVNTTRERYSIPFFLLPSHDVEVKPLE 310

Query: 330 ---SKSEPGVYRRFSFREFR-NQVQEDVRK 334
              S   P  Y+ + + +F  ++ + D RK
Sbjct: 311 ELVSPENPPKYKGYKWGKFYVSRNRSDFRK 334

BLAST of Cmc12g0332491 vs. TAIR 10
Match: AT5G07200.1 (gibberellin 20-oxidase 3 )

HSP 1 Score: 167.5 bits (423), Expect = 1.8e-41
Identity = 99/289 (34.26%), Postives = 138/289 (47.75%), Query Frame = 0

Query: 42  VEEWDLPVVDLGRLTGGKVEEEEECKNDIIRASKEWGFFQVVNHGISNQLLAKMRAKQIE 101
           V+   +P++DL     G      E    + +A+ + GFF + NHG+   LL++       
Sbjct: 53  VQPLQVPLIDLAGFLSGDSCLASEATRLVSKAATKHGFFLITNHGVDESLLSRAYLHMDS 112

Query: 102 LFKQPFERKSKEDQFSNFSAGSYRWGTPS--ATSI-----TQLSWSEAFHVSLS------ 161
            FK P   K K  +         +WG  S  A+S      ++L W E      S      
Sbjct: 113 FFKAPACEKQKAQR---------KWGESSGYASSFVGRFSSKLPWKETLSFKFSPEEKIH 172

Query: 162 -----DILGSNYGSD-DDLRSTMEEYAGKVSRLAQKLAEILGENLGRSSKFFIENCVPST 221
                D +    G   +D     +EYA  ++ L+ K+ E+LG +LG   ++F E    S 
Sbjct: 173 SQTVKDFVSKKMGDGYEDFGKVYQEYAEAMNTLSLKIMELLGMSLGVERRYFKEFFEDSD 232

Query: 222 CYLRMNRYPPCPVQGQIFGLMPHTDSDFLTILHQDQVGGLELVKDGKWIAVKPNPQALIV 281
              R+N YP C       G  PH D   LTILHQDQVGGL++  D KW ++ PNP A +V
Sbjct: 233 SIFRLNYYPQCKQPELALGTGPHCDPTSLTILHQDQVGGLQVFVDNKWQSIPPNPHAFVV 292

Query: 282 NIGDLFQVWSNDEYKSVEHRVVTNSRKERYSIAYFLCPWSETVIKSKSE 312
           NIGD F   +N  YKS  HR V NS +ER + A+FLCP  E V+K   E
Sbjct: 293 NIGDTFMALTNGRYKSCLHRAVVNSERERKTFAFFLCPKGEKVVKPPEE 332