Cmc12g0325721 (gene) Melon (Charmono) v1.1

Overview
NameCmc12g0325721
Typegene
OrganismCucumis melo L. var. cantalupensis cv. Charmono (Melon (Charmono) v1.1)
DescriptionUbiquinol oxidase
LocationCMiso1.1chr12: 15961509 .. 15965043 (+)
RNA-Seq ExpressionCmc12g0325721
SyntenyCmc12g0325721
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CACAAGATTATAAAACTTGATGGAGGTTGACAAACAAGGGAAATGGCCTCGGGAAAGTGCCAACTGCCGGCTAACAATTCTTCCGGGGTTCAGCCCCATACTTCCCCACTTTCTCTTCCACGATTCCAACCTTGATCTCCAAATCCTACCCCTCCCTCCAAAGCTATGTCAATCGCTCTGTCTTCTTCCTCTTCGGCAGTTTTTGCTATCTCCTCCGTTGAATTGAAGCCAAGCAACACCAAGAATGTTACCTCCTTCAGAATACCCACTTCCTTCTCTGCACGCCCTTTAATTTCCAGGTAACGCCTACACGGATTTTATCAGCTTCATTGATTGTCGTATCTTGTGTTTCTGTGAATGTTTCTTAGTGAGCTGAGAAGGGTTTTTTTCCAGGATTCAGTTTTGGGGTTGATTTCGGAATTTCTAAGCTCAATTTCGTTTTCTTTCTTTGAAAAATTGTCGAGGTGAATGATTGGTTTTGTCATTTTTATCATTTTCCCCCATCAATTGGGATTGTTGATGTAGAAAATCATGTCGGGTTATGGCAACTGTTCTAAAGGAAGATGAGGAAAAAGTGGTTTTGGAGGAATCATTCCCTCCCAAGGCTTCTCCTGTTCAAGACGGGGAAGGAGGCAGTGGCCAAGGACCACAAAGTTCATCATCCAATGGCTTGGATAAATGGATCATTAAGCTTGAACAATCTATTAATGTTCTTCTCACGGTAATATCAGTCCACTGTTCTATGCTTGTGCTTAATTGGTTCTGCAATACTTGAGATGTTGTAGCGTGACTCTTTATTAATGTTTCTAGGATTCGGTAATCAAAATTCTTGATGCATTTTACCACGATAGACACTACGCAAGGTTCTTTGTGTTGGAAACTATTGCAAGGGTTCCCTATTTTGGTGAGCTTTTAATTGCTTCATGTCTACCACTTTGTTAACACCTTGTTGATTTCTTCCTTGAAACAGAGGTGTTTCGTTTGGTATCTCATCATCCAGATTTTATTTTTCTGATTTCAGATTCAGTTAATCAAATGAATTTAGTAAACATCCATTTGCAGATTTGTTTTTGAAAATTTCTTTTGGATTTTGAGATCCTAAACACATTCTCGAAAACAGTGGAAAAATAAAATATAACTTTGATTTTCATCTAATCCCTTGTTTTCAAATTTGTGTTTTTAGATCACTAACCAAATATTGCTCCCTACTCAATAATGGTAAAGTCTGTTATCTGAATGCAGTGTGGCGGGCTAAAAAAACAATTTTATGTCACCTAATGAAGAACATTCTCATTAGCACAAAAATGGGCTCTTTAATAAATATGTAGATTGATGATTAATCTAAAATAATTGGTTCTGCTACTATTTTAATTTTGGAAGCCAGATTGTACAGCTTCTGTGGATGTTTAGTTGAGGATGAGTACAGTAGTGTCTCGTTTGTAGTTGTTTCTAATACCTCCCTTTAAAATTGCAGCGTTTGTGTCAGTTCTTCATATGTACGAAAGTTTTGGGTGGTGGAGAAGAGCAGATTATCTGAAAGTTCATTTTGCTGAGAGCTGGAATGAGATGCATCACTTGCTGATCATGGAGGTACTAATTATCTTGCATTATTTCATCCTGGTTAGAGTTGGAAGTATTCTCTTTTCTGTTTGCTGGTATGTTGAAGGACTGTATTGGTGATAGATGAAGGCTCTGTAGGACTTCGTCTTTCCTACACAATTTTCCTTTCCAACTATCTTGTGGACTGTTTTACTTTGTGAACCCATTTAGACATTAAATTATTGAATACATTAACTCATTTTCTGACTTGAGTGCAGGAACTGGGGGGCAACGATTGGTGGTTTGATCGTTTTCTTGCTCAACATATTGCTGTTGCTTATTACTTTATGACAGTATTTATGTACATGATTAGCCCAAGAATGGCATGTAAGTATTGTTGGAGCTGATAGATAAAACTGCTTATACACTCTTTCTTGCATCCTCTTGTGGGCTTGTTCAGTTCCCTCTTTTAACTGTAAGAAGTTACCATGAAAATAGAGAATTGAGTTATCTTTCCTTAGTAAAACTTACAAAGGAATTAAAATGTGAATTGCAGTTTGGCATCCGTTTTGGTGTATAACTATAATAAGTGGCTAGCTACTTTGGGACACATTTCTACTTCGATGTCCTTTTAACTTCTTCAAGTGTTTTGTATTAAACTTCTAATGGCATATTTCTCTGCAGATCACTTTTCTGAATGTGTTGAAAGCCATGCATTTTCAACTTATGATAAATTTTTAAAGGCCGAAGGAGGTATGTTTGTTGTGATCTCGATCGTTTTCTAGTTAACATTACATTTCATTTGGGAATATAGTTCTGATGTGTTCCATTCCTCTTGTTTATCTATAGAGGAGTTAAAAAAACAGCCTGCTCCTGAGGTTGCCGTGAAGTATTACACTGAAGGAGATTTATACTTATTTGGTAAATTCCATCTTTCTTACCACATGCACAGCCCTTTTGTTCCAAGTCAAACTGACTAACGAATTGACTTCATTTAGATGAATTTCAAACTTCGAGAGCTCCCAAATCACGAAGACCGAAAATAGGTAAAATTTGAAATCTGGTTTAGTTACTCTACTTCATAGTGCAAATGTCAGCTCTAGTACAGCCATGAAGGCATGACTTAAACAAGAAAATGTTTTTTCCCCTAAAAAGGGTTCACTAAAATAAGAGGAGTAGAGGCACAAATCATGTCTATGCGGTTCTAGTTCTTTTCTTTTCTTTTCTTTTTCATTGAAGTTCAAGTTTCTTCGGTTTACTAAAAGAGCCCTCAAATCTCTGGATGATAATATAATAGTTATGGTTGATGTTGGTGGTAGACGGTCCTATTGTGCACGTGTTTTCTGTGTTTGAAAGTTTGCTTTGACCAAATATTAATATGAGGTTATGTTTGGAAGTAATTTTGAAATGAATAAAGCACTTTTTCATGTTTAAGATCACTGAAAAATGTATCTTTAATCTTTGAAAATCACTTCAATATTTGATTTGATTTCCCTTCGTTATTCTAGAAAGCTTGTACGATGTTTTCGTGAACATCAGAGATGATGAAGCTGAGCATTGTAAGACGATGAGAGCCTGTCAATCACATGGGAATCTCCGGTCGCCACATTCTTATTCAGAGAGCTGCCCTGAAGATGATGAGCCACTCTGCAACTTAGCTGAAGCAGATTGTGAAGGCATTGTAGACTGTATAAAGAAATCCATTACTTCTTCCCCTCCGGTAAAGCAAAAGCTATGATATATATACATAGAAAGAAAATGTAAGTTCGCTTAGATATAGCAAATATCTTTGTCACATTCATTCGGGAGAAAAAAATCTAAGGTCCACACATAGATCACTTGATGATATTATTAAAGGTGACGATGCGCCTATCTTTGTAATAAAGTGGTTATAGTGATCAGAGTTTCAAAATTCTAATGGTATGCAAGTGCCCAGGAAAAAGTGACTTTTCTATTTCACTATTCAACCTGCGGGACTCTCTTGGGACTTTGAGTT

mRNA sequence

CACAAGATTATAAAACTTGATGGAGGTTGACAAACAAGGGAAATGGCCTCGGGAAAGTGCCAACTGCCGGCTAACAATTCTTCCGGGGTTCAGCCCCATACTTCCCCACTTTCTCTTCCACGATTCCAACCTTGATCTCCAAATCCTACCCCTCCCTCCAAAGCTATGTCAATCGCTCTGTCTTCTTCCTCTTCGGCAGTTTTTGCTATCTCCTCCGTTGAATTGAAGCCAAGCAACACCAAGAATGTTACCTCCTTCAGAATACCCACTTCCTTCTCTGCACGCCCTTTAATTTCCAGAAAATCATGTCGGGTTATGGCAACTGTTCTAAAGGAAGATGAGGAAAAAGTGGTTTTGGAGGAATCATTCCCTCCCAAGGCTTCTCCTGTTCAAGACGGGGAAGGAGGCAGTGGCCAAGGACCACAAAGTTCATCATCCAATGGCTTGGATAAATGGATCATTAAGCTTGAACAATCTATTAATGTTCTTCTCACGGATTCGGTAATCAAAATTCTTGATGCATTTTACCACGATAGACACTACGCAAGGTTCTTTGTGTTGGAAACTATTGCAAGGGTTCCCTATTTTGCGTTTGTGTCAGTTCTTCATATGTACGAAAGTTTTGGGTGGTGGAGAAGAGCAGATTATCTGAAAGTTCATTTTGCTGAGAGCTGGAATGAGATGCATCACTTGCTGATCATGGAGGAACTGGGGGGCAACGATTGGTGGTTTGATCGTTTTCTTGCTCAACATATTGCTGTTGCTTATTACTTTATGACAGTATTTATGTACATGATTAGCCCAAGAATGGCATATCACTTTTCTGAATGTGTTGAAAGCCATGCATTTTCAACTTATGATAAATTTTTAAAGGCCGAAGGAGAGGAGTTAAAAAAACAGCCTGCTCCTGAGGTTGCCGTGAAGTATTACACTGAAGGAGATTTATACTTATTTGATGAATTTCAAACTTCGAGAGCTCCCAAATCACGAAGACCGAAAATAGAAAGCTTGTACGATGTTTTCGTGAACATCAGAGATGATGAAGCTGAGCATTGTAAGACGATGAGAGCCTGTCAATCACATGGGAATCTCCGGTCGCCACATTCTTATTCAGAGAGCTGCCCTGAAGATGATGAGCCACTCTGCAACTTAGCTGAAGCAGATTGTGAAGGCATTGTAGACTGTATAAAGAAATCCATTACTTCTTCCCCTCCGGTAAAGCAAAAGCTATGATATATATACATAGAAAGAAAATGTAAGTTCGCTTAGATATAGCAAATATCTTTGTCACATTCATTCGGGAGAAAAAAATCTAAGGTCCACACATAGATCACTTGATGATATTATTAAAGGTGACGATGCGCCTATCTTTGTAATAAAGTGGTTATAGTGATCAGAGTTTCAAAATTCTAATGGTATGCAAGTGCCCAGGAAAAAGTGACTTTTCTATTTCACTATTCAACCTGCGGGACTCTCTTGGGACTTTGAGTT

Coding sequence (CDS)

ATGTCAATCGCTCTGTCTTCTTCCTCTTCGGCAGTTTTTGCTATCTCCTCCGTTGAATTGAAGCCAAGCAACACCAAGAATGTTACCTCCTTCAGAATACCCACTTCCTTCTCTGCACGCCCTTTAATTTCCAGAAAATCATGTCGGGTTATGGCAACTGTTCTAAAGGAAGATGAGGAAAAAGTGGTTTTGGAGGAATCATTCCCTCCCAAGGCTTCTCCTGTTCAAGACGGGGAAGGAGGCAGTGGCCAAGGACCACAAAGTTCATCATCCAATGGCTTGGATAAATGGATCATTAAGCTTGAACAATCTATTAATGTTCTTCTCACGGATTCGGTAATCAAAATTCTTGATGCATTTTACCACGATAGACACTACGCAAGGTTCTTTGTGTTGGAAACTATTGCAAGGGTTCCCTATTTTGCGTTTGTGTCAGTTCTTCATATGTACGAAAGTTTTGGGTGGTGGAGAAGAGCAGATTATCTGAAAGTTCATTTTGCTGAGAGCTGGAATGAGATGCATCACTTGCTGATCATGGAGGAACTGGGGGGCAACGATTGGTGGTTTGATCGTTTTCTTGCTCAACATATTGCTGTTGCTTATTACTTTATGACAGTATTTATGTACATGATTAGCCCAAGAATGGCATATCACTTTTCTGAATGTGTTGAAAGCCATGCATTTTCAACTTATGATAAATTTTTAAAGGCCGAAGGAGAGGAGTTAAAAAAACAGCCTGCTCCTGAGGTTGCCGTGAAGTATTACACTGAAGGAGATTTATACTTATTTGATGAATTTCAAACTTCGAGAGCTCCCAAATCACGAAGACCGAAAATAGAAAGCTTGTACGATGTTTTCGTGAACATCAGAGATGATGAAGCTGAGCATTGTAAGACGATGAGAGCCTGTCAATCACATGGGAATCTCCGGTCGCCACATTCTTATTCAGAGAGCTGCCCTGAAGATGATGAGCCACTCTGCAACTTAGCTGAAGCAGATTGTGAAGGCATTGTAGACTGTATAAAGAAATCCATTACTTCTTCCCCTCCGGTAAAGCAAAAGCTATGA

Protein sequence

MSIALSSSSSAVFAISSVELKPSNTKNVTSFRIPTSFSARPLISRKSCRVMATVLKEDEEKVVLEESFPPKASPVQDGEGGSGQGPQSSSSNGLDKWIIKLEQSINVLLTDSVIKILDAFYHDRHYARFFVLETIARVPYFAFVSVLHMYESFGWWRRADYLKVHFAESWNEMHHLLIMEELGGNDWWFDRFLAQHIAVAYYFMTVFMYMISPRMAYHFSECVESHAFSTYDKFLKAEGEELKKQPAPEVAVKYYTEGDLYLFDEFQTSRAPKSRRPKIESLYDVFVNIRDDEAEHCKTMRACQSHGNLRSPHSYSESCPEDDEPLCNLAEADCEGIVDCIKKSITSSPPVKQKL
Homology
BLAST of Cmc12g0325721 vs. NCBI nr
Match: XP_008458986.1 (PREDICTED: ubiquinol oxidase 4, chloroplastic/chromoplastic-like [Cucumis melo] >KAA0054287.1 ubiquinol oxidase 4 [Cucumis melo var. makuwa] >TYK12325.1 ubiquinol oxidase 4 [Cucumis melo var. makuwa])

HSP 1 Score: 709.9 bits (1831), Expect = 1.1e-200
Identity = 355/355 (100.00%), Postives = 355/355 (100.00%), Query Frame = 0

Query: 1   MSIALSSSSSAVFAISSVELKPSNTKNVTSFRIPTSFSARPLISRKSCRVMATVLKEDEE 60
           MSIALSSSSSAVFAISSVELKPSNTKNVTSFRIPTSFSARPLISRKSCRVMATVLKEDEE
Sbjct: 1   MSIALSSSSSAVFAISSVELKPSNTKNVTSFRIPTSFSARPLISRKSCRVMATVLKEDEE 60

Query: 61  KVVLEESFPPKASPVQDGEGGSGQGPQSSSSNGLDKWIIKLEQSINVLLTDSVIKILDAF 120
           KVVLEESFPPKASPVQDGEGGSGQGPQSSSSNGLDKWIIKLEQSINVLLTDSVIKILDAF
Sbjct: 61  KVVLEESFPPKASPVQDGEGGSGQGPQSSSSNGLDKWIIKLEQSINVLLTDSVIKILDAF 120

Query: 121 YHDRHYARFFVLETIARVPYFAFVSVLHMYESFGWWRRADYLKVHFAESWNEMHHLLIME 180
           YHDRHYARFFVLETIARVPYFAFVSVLHMYESFGWWRRADYLKVHFAESWNEMHHLLIME
Sbjct: 121 YHDRHYARFFVLETIARVPYFAFVSVLHMYESFGWWRRADYLKVHFAESWNEMHHLLIME 180

Query: 181 ELGGNDWWFDRFLAQHIAVAYYFMTVFMYMISPRMAYHFSECVESHAFSTYDKFLKAEGE 240
           ELGGNDWWFDRFLAQHIAVAYYFMTVFMYMISPRMAYHFSECVESHAFSTYDKFLKAEGE
Sbjct: 181 ELGGNDWWFDRFLAQHIAVAYYFMTVFMYMISPRMAYHFSECVESHAFSTYDKFLKAEGE 240

Query: 241 ELKKQPAPEVAVKYYTEGDLYLFDEFQTSRAPKSRRPKIESLYDVFVNIRDDEAEHCKTM 300
           ELKKQPAPEVAVKYYTEGDLYLFDEFQTSRAPKSRRPKIESLYDVFVNIRDDEAEHCKTM
Sbjct: 241 ELKKQPAPEVAVKYYTEGDLYLFDEFQTSRAPKSRRPKIESLYDVFVNIRDDEAEHCKTM 300

Query: 301 RACQSHGNLRSPHSYSESCPEDDEPLCNLAEADCEGIVDCIKKSITSSPPVKQKL 356
           RACQSHGNLRSPHSYSESCPEDDEPLCNLAEADCEGIVDCIKKSITSSPPVKQKL
Sbjct: 301 RACQSHGNLRSPHSYSESCPEDDEPLCNLAEADCEGIVDCIKKSITSSPPVKQKL 355

BLAST of Cmc12g0325721 vs. NCBI nr
Match: XP_004145426.1 (ubiquinol oxidase 4, chloroplastic/chromoplastic [Cucumis sativus])

HSP 1 Score: 693.3 bits (1788), Expect = 1.1e-195
Identity = 342/355 (96.34%), Postives = 350/355 (98.59%), Query Frame = 0

Query: 1   MSIALSSSSSAVFAISSVELKPSNTKNVTSFRIPTSFSARPLISRKSCRVMATVLKEDEE 60
           MSIALSSSSS +FA SSVELKP NTKN+ SFRIPTSFSARPLISRKSCRVMATVLKEDEE
Sbjct: 1   MSIALSSSSSPLFAFSSVELKPINTKNLASFRIPTSFSARPLISRKSCRVMATVLKEDEE 60

Query: 61  KVVLEESFPPKASPVQDGEGGSGQGPQSSSSNGLDKWIIKLEQSINVLLTDSVIKILDAF 120
           KVVLEESFPPKASP+QDGEGGSGQGP+SSSSNG DKWI+KLEQS+NVLLTDSVIKILDAF
Sbjct: 61  KVVLEESFPPKASPIQDGEGGSGQGPESSSSNGFDKWIVKLEQSVNVLLTDSVIKILDAF 120

Query: 121 YHDRHYARFFVLETIARVPYFAFVSVLHMYESFGWWRRADYLKVHFAESWNEMHHLLIME 180
           YHDRHYARFFVLETIARVPYFAFVSVLHMYESFGWWRRADYLKVHFAESWNEMHHLLIME
Sbjct: 121 YHDRHYARFFVLETIARVPYFAFVSVLHMYESFGWWRRADYLKVHFAESWNEMHHLLIME 180

Query: 181 ELGGNDWWFDRFLAQHIAVAYYFMTVFMYMISPRMAYHFSECVESHAFSTYDKFLKAEGE 240
           ELGGNDWWFDRFLAQHIAVAYYFMTVFMYMISPRMAYHFSECVESHAFSTYDKFLKAEGE
Sbjct: 181 ELGGNDWWFDRFLAQHIAVAYYFMTVFMYMISPRMAYHFSECVESHAFSTYDKFLKAEGE 240

Query: 241 ELKKQPAPEVAVKYYTEGDLYLFDEFQTSRAPKSRRPKIESLYDVFVNIRDDEAEHCKTM 300
           ELKKQPAPEVAVKYYTEGDLYLFDEFQTSRAPKSRRPKIESLYDVF+NIRDDEAEHCKTM
Sbjct: 241 ELKKQPAPEVAVKYYTEGDLYLFDEFQTSRAPKSRRPKIESLYDVFMNIRDDEAEHCKTM 300

Query: 301 RACQSHGNLRSPHSYSESCPEDDEPLCNLAEADCEGIVDCIKKSITSSPPVKQKL 356
           RACQSHGNLRSPHSYSESCPE+DEPLCNLAEADCEGIVDCIKKSITSSPPVKQKL
Sbjct: 301 RACQSHGNLRSPHSYSESCPEEDEPLCNLAEADCEGIVDCIKKSITSSPPVKQKL 355

BLAST of Cmc12g0325721 vs. NCBI nr
Match: XP_038893742.1 (ubiquinol oxidase 4, chloroplastic/chromoplastic-like [Benincasa hispida])

HSP 1 Score: 660.2 bits (1702), Expect = 9.9e-186
Identity = 329/360 (91.39%), Postives = 341/360 (94.72%), Query Frame = 0

Query: 1   MSIALSSSSSAVFAISSVELKPSNTKNVTSFRIPTSFS-----ARPLISRKSCRVMATVL 60
           MS A SS SSAVFAISSV+LKP+NTKNV SFRIPTSF      A PLISRKSCRV+ATVL
Sbjct: 1   MSTAWSSLSSAVFAISSVQLKPNNTKNVASFRIPTSFPRRSFVAHPLISRKSCRVLATVL 60

Query: 61  KEDEEKVVLEESFPPKASPVQDGEGGSGQGPQSSSSNGLDKWIIKLEQSINVLLTDSVIK 120
           KEDEEKVV+E+SFPPKASPVQDGEGGS +GP+ S SNG DKWIIKLEQS+NV LTDSVI+
Sbjct: 61  KEDEEKVVVEDSFPPKASPVQDGEGGSNEGPEISPSNGFDKWIIKLEQSVNVFLTDSVIR 120

Query: 121 ILDAFYHDRHYARFFVLETIARVPYFAFVSVLHMYESFGWWRRADYLKVHFAESWNEMHH 180
           ILDAFYHDRHYARFFVLETIARVPYFAF+SVLHMYESFGWWRRADYLKVHFAESWNEMHH
Sbjct: 121 ILDAFYHDRHYARFFVLETIARVPYFAFLSVLHMYESFGWWRRADYLKVHFAESWNEMHH 180

Query: 181 LLIMEELGGNDWWFDRFLAQHIAVAYYFMTVFMYMISPRMAYHFSECVESHAFSTYDKFL 240
           LLIMEELGGNDWWFDRFLAQHIAVAYYFMTVFMYMISPRMAYHFSECVESHAFSTYDKFL
Sbjct: 181 LLIMEELGGNDWWFDRFLAQHIAVAYYFMTVFMYMISPRMAYHFSECVESHAFSTYDKFL 240

Query: 241 KAEGEELKKQPAPEVAVKYYTEGDLYLFDEFQTSRAPKSRRPKIESLYDVFVNIRDDEAE 300
           KA+GEELKK PAPEVAVKYYTEGDLYLFDEFQTSRAPKSRRPKIESLYDVFVNIRDDEAE
Sbjct: 241 KAQGEELKKLPAPEVAVKYYTEGDLYLFDEFQTSRAPKSRRPKIESLYDVFVNIRDDEAE 300

Query: 301 HCKTMRACQSHGNLRSPHSYSESCPEDDEPLCNLAEADCEGIVDCIKKSITSSPPVKQKL 356
           HCKTMRACQSHGNLRSPHSYSESCPEDDEPLCNL+EADCEGIVDCIKKSIT S PVKQKL
Sbjct: 301 HCKTMRACQSHGNLRSPHSYSESCPEDDEPLCNLSEADCEGIVDCIKKSITFSHPVKQKL 360

BLAST of Cmc12g0325721 vs. NCBI nr
Match: XP_022994224.1 (ubiquinol oxidase 4, chloroplastic/chromoplastic [Cucurbita maxima])

HSP 1 Score: 632.1 bits (1629), Expect = 2.9e-177
Identity = 315/361 (87.26%), Postives = 335/361 (92.80%), Query Frame = 0

Query: 1   MSIALSSSSSAVFAISSVELKPSNTKNVTSFRIPT-----SFSARPLISRKSCRVMATVL 60
           MSIA +SSSSA+F  SSV+L P NTKN+ SFRI T     SFS+RPL+SRK CRV+ATVL
Sbjct: 1   MSIAWTSSSSALFGTSSVQLNPYNTKNIASFRIGTAFSRRSFSSRPLVSRKLCRVLATVL 60

Query: 61  KEDEEKVVLEESFPPKASPVQDGEGGSGQGPQSSSSNGLDKWIIKLEQSINVLLTDSVIK 120
           KEDEE+VV+EESFPPKASPV  GEGGS  G ++SSSN LDKWIIKLEQS+NVLLTDSVI+
Sbjct: 61  KEDEEEVVIEESFPPKASPVDRGEGGSEGGAENSSSNALDKWIIKLEQSVNVLLTDSVIR 120

Query: 121 ILDAFYHDRHYARFFVLETIARVPYFAFVSVLHMYESFGWWRRADYLKVHFAESWNEMHH 180
           ILDAFYHDRHY RFFVLETIARVPYFAF+SVLHMYESFGWWRRADYLKVHFAESWNEMHH
Sbjct: 121 ILDAFYHDRHYPRFFVLETIARVPYFAFLSVLHMYESFGWWRRADYLKVHFAESWNEMHH 180

Query: 181 LLIMEELGGNDWWFDRFLAQHIAVAYYFMTVFMYMISPRMAYHFSECVESHAFSTYDKFL 240
           LLIMEELGGNDWWFDRFLAQHIAVAYYFMTV MYMISPRMAYH SECVESHAFSTYDKFL
Sbjct: 181 LLIMEELGGNDWWFDRFLAQHIAVAYYFMTVVMYMISPRMAYHLSECVESHAFSTYDKFL 240

Query: 241 KAEGEELKKQPAPEVAVKYYTEGDLYLFDEFQTSRAPKSRRPKIESLYDVFVNIRDDEAE 300
           KA+GEELKK PAPEVAVKYYTEGDLYLFDEFQTSRAP SRRPKI++LYDVFVNIRDDEAE
Sbjct: 241 KAQGEELKKLPAPEVAVKYYTEGDLYLFDEFQTSRAPNSRRPKIDNLYDVFVNIRDDEAE 300

Query: 301 HCKTMRACQSHGNLRSPHSYSESCPEDDEPLCNLAE-ADCEGIVDCIKKSITSSPPVKQK 356
           HCKTMRACQSHGNLRSPHSYSESCPEDDEPLCNL E ADCEGIVDC+KK++TSSPPVKQK
Sbjct: 301 HCKTMRACQSHGNLRSPHSYSESCPEDDEPLCNLPEAADCEGIVDCLKKAVTSSPPVKQK 360

BLAST of Cmc12g0325721 vs. NCBI nr
Match: XP_022955227.1 (ubiquinol oxidase 4, chloroplastic/chromoplastic [Cucurbita moschata])

HSP 1 Score: 629.8 bits (1623), Expect = 1.4e-176
Identity = 316/361 (87.53%), Postives = 335/361 (92.80%), Query Frame = 0

Query: 1   MSIALSSSSSAVFAISSVELKPSNTKNVTSFRIPT-----SFSARPLISRKSCRVMATVL 60
           MSIA  SSSSA+F  SSV+LKP NT N+ SFRI T     SFS+RPL+SRKSCRV+ATVL
Sbjct: 1   MSIAWCSSSSALFGTSSVQLKPYNTINIDSFRIGTAFSRRSFSSRPLVSRKSCRVLATVL 60

Query: 61  KEDEEKVVLEESFPPKASPVQDGEGGSGQGPQSSSSNGLDKWIIKLEQSINVLLTDSVIK 120
           KEDEE+VV+EESFPPKASPV+ GEGGS  G ++SSSNGLDKWIIKLEQS+NVLLTDSVI+
Sbjct: 61  KEDEEEVVIEESFPPKASPVEKGEGGSEGGAENSSSNGLDKWIIKLEQSVNVLLTDSVIR 120

Query: 121 ILDAFYHDRHYARFFVLETIARVPYFAFVSVLHMYESFGWWRRADYLKVHFAESWNEMHH 180
           ILDAFYHDRHY RFFVLETIARVPYFAF+SVLHMYESFGWWRRADYLKVHFAESWNEMHH
Sbjct: 121 ILDAFYHDRHYPRFFVLETIARVPYFAFLSVLHMYESFGWWRRADYLKVHFAESWNEMHH 180

Query: 181 LLIMEELGGNDWWFDRFLAQHIAVAYYFMTVFMYMISPRMAYHFSECVESHAFSTYDKFL 240
           LLIMEELGGNDWWFDRFLAQHIAVAYYFMTV MYMISPRMAYH SECVESHAFSTYDKFL
Sbjct: 181 LLIMEELGGNDWWFDRFLAQHIAVAYYFMTVVMYMISPRMAYHLSECVESHAFSTYDKFL 240

Query: 241 KAEGEELKKQPAPEVAVKYYTEGDLYLFDEFQTSRAPKSRRPKIESLYDVFVNIRDDEAE 300
           KA+GEELKK PAPEVAVKYYTEGDLYLFDEFQTSRAP SRRPKIE+LYDVFVNIRDDEAE
Sbjct: 241 KAQGEELKKLPAPEVAVKYYTEGDLYLFDEFQTSRAPNSRRPKIENLYDVFVNIRDDEAE 300

Query: 301 HCKTMRACQSHGNLRSPHSYSESCPEDDEPLCNLAE-ADCEGIVDCIKKSITSSPPVKQK 356
           HCKTMRACQSHGNLRSPHSYSES PEDDEPLCNL E ADCEGIVDC+KK++TSSPPVK K
Sbjct: 301 HCKTMRACQSHGNLRSPHSYSESRPEDDEPLCNLPEAADCEGIVDCLKKAVTSSPPVKPK 360

BLAST of Cmc12g0325721 vs. ExPASy Swiss-Prot
Match: Q56X52 (Ubiquinol oxidase 4, chloroplastic/chromoplastic OS=Arabidopsis thaliana OX=3702 GN=AOX4 PE=1 SV=2)

HSP 1 Score: 459.1 bits (1180), Expect = 4.4e-128
Identity = 230/311 (73.95%), Postives = 257/311 (82.64%), Query Frame = 0

Query: 38  SARPLISRKSCRVMATVLKEDEEKVVLEESFPPKASPVQDGEGGSGQGPQSSSSNGLDKW 97
           S+R L+ R + RV AT+L++DEEKVV+EESF  KA      E        SSS++  + W
Sbjct: 43  SSRRLLLRNNHRVQATILQDDEEKVVVEESF--KAETSTGTEPLEEPNMSSSSTSAFETW 102

Query: 98  IIKLEQSINVLLTDSVIKILDAFYHDRHYARFFVLETIARVPYFAFVSVLHMYESFGWWR 157
           IIKLEQ +NV LTDSVIKILD  Y DR YARFFVLETIARVPYFAF+SVLHMYE+FGWWR
Sbjct: 103 IIKLEQGVNVFLTDSVIKILDTLYRDRTYARFFVLETIARVPYFAFMSVLHMYETFGWWR 162

Query: 158 RADYLKVHFAESWNEMHHLLIMEELGGNDWWFDRFLAQHIAVAYYFMTVFMYMISPRMAY 217
           RADYLKVHFAESWNEMHHLLIMEELGGN WWFDRFLAQHIA  YYFMTVF+Y++SPRMAY
Sbjct: 163 RADYLKVHFAESWNEMHHLLIMEELGGNSWWFDRFLAQHIATFYYFMTVFLYILSPRMAY 222

Query: 218 HFSECVESHAFSTYDKFLKAEGEELKKQPAPEVAVKYYTEGDLYLFDEFQTSRAPKSRRP 277
           HFSECVESHA+ TYDKFLKA GEELK  PAP++AVKYYT GDLYLFDEFQTSR P +RRP
Sbjct: 223 HFSECVESHAYETYDKFLKASGEELKNMPAPDIAVKYYTGGDLYLFDEFQTSRTPNTRRP 282

Query: 278 KIESLYDVFVNIRDDEAEHCKTMRACQSHGNLRSPHSYSESCPEDDEPLCNL-AEADCEG 337
            IE+LYDVFVNIRDDEAEHCKTMRACQ+ G+LRSPHS  E    ++E  C +  EA CEG
Sbjct: 283 VIENLYDVFVNIRDDEAEHCKTMRACQTLGSLRSPHSILEDDDTEEESGCVVPEEAHCEG 342

Query: 338 IVDCIKKSITS 348
           IVDC+KKSITS
Sbjct: 343 IVDCLKKSITS 351

BLAST of Cmc12g0325721 vs. ExPASy Swiss-Prot
Match: Q8LEE7 (Ubiquinol oxidase 3, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=AOX3 PE=1 SV=2)

HSP 1 Score: 67.4 bits (163), Expect = 3.7e-10
Identity = 51/189 (26.98%), Postives = 86/189 (45.50%), Query Frame = 0

Query: 110 TDSVIKI-LDAFYHDRHYARFFVLETIARVPYFAFVSVLHMYESFGWWRRADYLKVHFAE 169
           T   +KI +  F+  +H     +LET+A VP      +LH+     +     ++K    E
Sbjct: 123 TVQTLKIPVQLFFQRKHMCHAMLLETVAAVPGMVGGMLLHLKSLRRFEHSGGWIKALLEE 182

Query: 170 SWNEMHHLLIMEELGGNDWWFDRFLAQHIAVAYYFMTVFMYMISPRMAYHFSECVESHAF 229
           + NE  HL+   EL     W++R +   +   ++      Y+ISP++A+  +  +E  A 
Sbjct: 183 AENERMHLMTFIEL-SQPKWYERAIVFTVQGVFFNAYFLAYVISPKLAHRITGYLEEEAV 242

Query: 230 STYDKFLK-AEGEELKKQPAPEVAVKYYTEGDLYLFDEFQTSRAPKSRRPKIESLYDVFV 289
           ++Y +FLK  +  + +  PAP +A+ Y+                   R PK  +L DV  
Sbjct: 243 NSYTEFLKDIDAGKFENSPAPAIAIDYW-------------------RLPKDATLRDVVY 291

Query: 290 NIRDDEAEH 297
            IR DEA H
Sbjct: 303 VIRADEAHH 291

BLAST of Cmc12g0325721 vs. ExPASy TrEMBL
Match: A0A5A7UI87 (Ubiquinol oxidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold302G00950 PE=3 SV=1)

HSP 1 Score: 709.9 bits (1831), Expect = 5.3e-201
Identity = 355/355 (100.00%), Postives = 355/355 (100.00%), Query Frame = 0

Query: 1   MSIALSSSSSAVFAISSVELKPSNTKNVTSFRIPTSFSARPLISRKSCRVMATVLKEDEE 60
           MSIALSSSSSAVFAISSVELKPSNTKNVTSFRIPTSFSARPLISRKSCRVMATVLKEDEE
Sbjct: 1   MSIALSSSSSAVFAISSVELKPSNTKNVTSFRIPTSFSARPLISRKSCRVMATVLKEDEE 60

Query: 61  KVVLEESFPPKASPVQDGEGGSGQGPQSSSSNGLDKWIIKLEQSINVLLTDSVIKILDAF 120
           KVVLEESFPPKASPVQDGEGGSGQGPQSSSSNGLDKWIIKLEQSINVLLTDSVIKILDAF
Sbjct: 61  KVVLEESFPPKASPVQDGEGGSGQGPQSSSSNGLDKWIIKLEQSINVLLTDSVIKILDAF 120

Query: 121 YHDRHYARFFVLETIARVPYFAFVSVLHMYESFGWWRRADYLKVHFAESWNEMHHLLIME 180
           YHDRHYARFFVLETIARVPYFAFVSVLHMYESFGWWRRADYLKVHFAESWNEMHHLLIME
Sbjct: 121 YHDRHYARFFVLETIARVPYFAFVSVLHMYESFGWWRRADYLKVHFAESWNEMHHLLIME 180

Query: 181 ELGGNDWWFDRFLAQHIAVAYYFMTVFMYMISPRMAYHFSECVESHAFSTYDKFLKAEGE 240
           ELGGNDWWFDRFLAQHIAVAYYFMTVFMYMISPRMAYHFSECVESHAFSTYDKFLKAEGE
Sbjct: 181 ELGGNDWWFDRFLAQHIAVAYYFMTVFMYMISPRMAYHFSECVESHAFSTYDKFLKAEGE 240

Query: 241 ELKKQPAPEVAVKYYTEGDLYLFDEFQTSRAPKSRRPKIESLYDVFVNIRDDEAEHCKTM 300
           ELKKQPAPEVAVKYYTEGDLYLFDEFQTSRAPKSRRPKIESLYDVFVNIRDDEAEHCKTM
Sbjct: 241 ELKKQPAPEVAVKYYTEGDLYLFDEFQTSRAPKSRRPKIESLYDVFVNIRDDEAEHCKTM 300

Query: 301 RACQSHGNLRSPHSYSESCPEDDEPLCNLAEADCEGIVDCIKKSITSSPPVKQKL 356
           RACQSHGNLRSPHSYSESCPEDDEPLCNLAEADCEGIVDCIKKSITSSPPVKQKL
Sbjct: 301 RACQSHGNLRSPHSYSESCPEDDEPLCNLAEADCEGIVDCIKKSITSSPPVKQKL 355

BLAST of Cmc12g0325721 vs. ExPASy TrEMBL
Match: A0A1S3C992 (Ubiquinol oxidase OS=Cucumis melo OX=3656 GN=LOC103498237 PE=3 SV=1)

HSP 1 Score: 709.9 bits (1831), Expect = 5.3e-201
Identity = 355/355 (100.00%), Postives = 355/355 (100.00%), Query Frame = 0

Query: 1   MSIALSSSSSAVFAISSVELKPSNTKNVTSFRIPTSFSARPLISRKSCRVMATVLKEDEE 60
           MSIALSSSSSAVFAISSVELKPSNTKNVTSFRIPTSFSARPLISRKSCRVMATVLKEDEE
Sbjct: 1   MSIALSSSSSAVFAISSVELKPSNTKNVTSFRIPTSFSARPLISRKSCRVMATVLKEDEE 60

Query: 61  KVVLEESFPPKASPVQDGEGGSGQGPQSSSSNGLDKWIIKLEQSINVLLTDSVIKILDAF 120
           KVVLEESFPPKASPVQDGEGGSGQGPQSSSSNGLDKWIIKLEQSINVLLTDSVIKILDAF
Sbjct: 61  KVVLEESFPPKASPVQDGEGGSGQGPQSSSSNGLDKWIIKLEQSINVLLTDSVIKILDAF 120

Query: 121 YHDRHYARFFVLETIARVPYFAFVSVLHMYESFGWWRRADYLKVHFAESWNEMHHLLIME 180
           YHDRHYARFFVLETIARVPYFAFVSVLHMYESFGWWRRADYLKVHFAESWNEMHHLLIME
Sbjct: 121 YHDRHYARFFVLETIARVPYFAFVSVLHMYESFGWWRRADYLKVHFAESWNEMHHLLIME 180

Query: 181 ELGGNDWWFDRFLAQHIAVAYYFMTVFMYMISPRMAYHFSECVESHAFSTYDKFLKAEGE 240
           ELGGNDWWFDRFLAQHIAVAYYFMTVFMYMISPRMAYHFSECVESHAFSTYDKFLKAEGE
Sbjct: 181 ELGGNDWWFDRFLAQHIAVAYYFMTVFMYMISPRMAYHFSECVESHAFSTYDKFLKAEGE 240

Query: 241 ELKKQPAPEVAVKYYTEGDLYLFDEFQTSRAPKSRRPKIESLYDVFVNIRDDEAEHCKTM 300
           ELKKQPAPEVAVKYYTEGDLYLFDEFQTSRAPKSRRPKIESLYDVFVNIRDDEAEHCKTM
Sbjct: 241 ELKKQPAPEVAVKYYTEGDLYLFDEFQTSRAPKSRRPKIESLYDVFVNIRDDEAEHCKTM 300

Query: 301 RACQSHGNLRSPHSYSESCPEDDEPLCNLAEADCEGIVDCIKKSITSSPPVKQKL 356
           RACQSHGNLRSPHSYSESCPEDDEPLCNLAEADCEGIVDCIKKSITSSPPVKQKL
Sbjct: 301 RACQSHGNLRSPHSYSESCPEDDEPLCNLAEADCEGIVDCIKKSITSSPPVKQKL 355

BLAST of Cmc12g0325721 vs. ExPASy TrEMBL
Match: A0A0A0M130 (Ubiquinol oxidase OS=Cucumis sativus OX=3659 GN=Csa_1G699610 PE=3 SV=1)

HSP 1 Score: 693.3 bits (1788), Expect = 5.1e-196
Identity = 342/355 (96.34%), Postives = 350/355 (98.59%), Query Frame = 0

Query: 1   MSIALSSSSSAVFAISSVELKPSNTKNVTSFRIPTSFSARPLISRKSCRVMATVLKEDEE 60
           MSIALSSSSS +FA SSVELKP NTKN+ SFRIPTSFSARPLISRKSCRVMATVLKEDEE
Sbjct: 1   MSIALSSSSSPLFAFSSVELKPINTKNLASFRIPTSFSARPLISRKSCRVMATVLKEDEE 60

Query: 61  KVVLEESFPPKASPVQDGEGGSGQGPQSSSSNGLDKWIIKLEQSINVLLTDSVIKILDAF 120
           KVVLEESFPPKASP+QDGEGGSGQGP+SSSSNG DKWI+KLEQS+NVLLTDSVIKILDAF
Sbjct: 61  KVVLEESFPPKASPIQDGEGGSGQGPESSSSNGFDKWIVKLEQSVNVLLTDSVIKILDAF 120

Query: 121 YHDRHYARFFVLETIARVPYFAFVSVLHMYESFGWWRRADYLKVHFAESWNEMHHLLIME 180
           YHDRHYARFFVLETIARVPYFAFVSVLHMYESFGWWRRADYLKVHFAESWNEMHHLLIME
Sbjct: 121 YHDRHYARFFVLETIARVPYFAFVSVLHMYESFGWWRRADYLKVHFAESWNEMHHLLIME 180

Query: 181 ELGGNDWWFDRFLAQHIAVAYYFMTVFMYMISPRMAYHFSECVESHAFSTYDKFLKAEGE 240
           ELGGNDWWFDRFLAQHIAVAYYFMTVFMYMISPRMAYHFSECVESHAFSTYDKFLKAEGE
Sbjct: 181 ELGGNDWWFDRFLAQHIAVAYYFMTVFMYMISPRMAYHFSECVESHAFSTYDKFLKAEGE 240

Query: 241 ELKKQPAPEVAVKYYTEGDLYLFDEFQTSRAPKSRRPKIESLYDVFVNIRDDEAEHCKTM 300
           ELKKQPAPEVAVKYYTEGDLYLFDEFQTSRAPKSRRPKIESLYDVF+NIRDDEAEHCKTM
Sbjct: 241 ELKKQPAPEVAVKYYTEGDLYLFDEFQTSRAPKSRRPKIESLYDVFMNIRDDEAEHCKTM 300

Query: 301 RACQSHGNLRSPHSYSESCPEDDEPLCNLAEADCEGIVDCIKKSITSSPPVKQKL 356
           RACQSHGNLRSPHSYSESCPE+DEPLCNLAEADCEGIVDCIKKSITSSPPVKQKL
Sbjct: 301 RACQSHGNLRSPHSYSESCPEEDEPLCNLAEADCEGIVDCIKKSITSSPPVKQKL 355

BLAST of Cmc12g0325721 vs. ExPASy TrEMBL
Match: A0A6J1K2B2 (Ubiquinol oxidase OS=Cucurbita maxima OX=3661 GN=LOC111490026 PE=3 SV=1)

HSP 1 Score: 632.1 bits (1629), Expect = 1.4e-177
Identity = 315/361 (87.26%), Postives = 335/361 (92.80%), Query Frame = 0

Query: 1   MSIALSSSSSAVFAISSVELKPSNTKNVTSFRIPT-----SFSARPLISRKSCRVMATVL 60
           MSIA +SSSSA+F  SSV+L P NTKN+ SFRI T     SFS+RPL+SRK CRV+ATVL
Sbjct: 1   MSIAWTSSSSALFGTSSVQLNPYNTKNIASFRIGTAFSRRSFSSRPLVSRKLCRVLATVL 60

Query: 61  KEDEEKVVLEESFPPKASPVQDGEGGSGQGPQSSSSNGLDKWIIKLEQSINVLLTDSVIK 120
           KEDEE+VV+EESFPPKASPV  GEGGS  G ++SSSN LDKWIIKLEQS+NVLLTDSVI+
Sbjct: 61  KEDEEEVVIEESFPPKASPVDRGEGGSEGGAENSSSNALDKWIIKLEQSVNVLLTDSVIR 120

Query: 121 ILDAFYHDRHYARFFVLETIARVPYFAFVSVLHMYESFGWWRRADYLKVHFAESWNEMHH 180
           ILDAFYHDRHY RFFVLETIARVPYFAF+SVLHMYESFGWWRRADYLKVHFAESWNEMHH
Sbjct: 121 ILDAFYHDRHYPRFFVLETIARVPYFAFLSVLHMYESFGWWRRADYLKVHFAESWNEMHH 180

Query: 181 LLIMEELGGNDWWFDRFLAQHIAVAYYFMTVFMYMISPRMAYHFSECVESHAFSTYDKFL 240
           LLIMEELGGNDWWFDRFLAQHIAVAYYFMTV MYMISPRMAYH SECVESHAFSTYDKFL
Sbjct: 181 LLIMEELGGNDWWFDRFLAQHIAVAYYFMTVVMYMISPRMAYHLSECVESHAFSTYDKFL 240

Query: 241 KAEGEELKKQPAPEVAVKYYTEGDLYLFDEFQTSRAPKSRRPKIESLYDVFVNIRDDEAE 300
           KA+GEELKK PAPEVAVKYYTEGDLYLFDEFQTSRAP SRRPKI++LYDVFVNIRDDEAE
Sbjct: 241 KAQGEELKKLPAPEVAVKYYTEGDLYLFDEFQTSRAPNSRRPKIDNLYDVFVNIRDDEAE 300

Query: 301 HCKTMRACQSHGNLRSPHSYSESCPEDDEPLCNLAE-ADCEGIVDCIKKSITSSPPVKQK 356
           HCKTMRACQSHGNLRSPHSYSESCPEDDEPLCNL E ADCEGIVDC+KK++TSSPPVKQK
Sbjct: 301 HCKTMRACQSHGNLRSPHSYSESCPEDDEPLCNLPEAADCEGIVDCLKKAVTSSPPVKQK 360

BLAST of Cmc12g0325721 vs. ExPASy TrEMBL
Match: A0A6J1GSZ7 (Ubiquinol oxidase OS=Cucurbita moschata OX=3662 GN=LOC111457254 PE=3 SV=1)

HSP 1 Score: 629.8 bits (1623), Expect = 7.0e-177
Identity = 316/361 (87.53%), Postives = 335/361 (92.80%), Query Frame = 0

Query: 1   MSIALSSSSSAVFAISSVELKPSNTKNVTSFRIPT-----SFSARPLISRKSCRVMATVL 60
           MSIA  SSSSA+F  SSV+LKP NT N+ SFRI T     SFS+RPL+SRKSCRV+ATVL
Sbjct: 1   MSIAWCSSSSALFGTSSVQLKPYNTINIDSFRIGTAFSRRSFSSRPLVSRKSCRVLATVL 60

Query: 61  KEDEEKVVLEESFPPKASPVQDGEGGSGQGPQSSSSNGLDKWIIKLEQSINVLLTDSVIK 120
           KEDEE+VV+EESFPPKASPV+ GEGGS  G ++SSSNGLDKWIIKLEQS+NVLLTDSVI+
Sbjct: 61  KEDEEEVVIEESFPPKASPVEKGEGGSEGGAENSSSNGLDKWIIKLEQSVNVLLTDSVIR 120

Query: 121 ILDAFYHDRHYARFFVLETIARVPYFAFVSVLHMYESFGWWRRADYLKVHFAESWNEMHH 180
           ILDAFYHDRHY RFFVLETIARVPYFAF+SVLHMYESFGWWRRADYLKVHFAESWNEMHH
Sbjct: 121 ILDAFYHDRHYPRFFVLETIARVPYFAFLSVLHMYESFGWWRRADYLKVHFAESWNEMHH 180

Query: 181 LLIMEELGGNDWWFDRFLAQHIAVAYYFMTVFMYMISPRMAYHFSECVESHAFSTYDKFL 240
           LLIMEELGGNDWWFDRFLAQHIAVAYYFMTV MYMISPRMAYH SECVESHAFSTYDKFL
Sbjct: 181 LLIMEELGGNDWWFDRFLAQHIAVAYYFMTVVMYMISPRMAYHLSECVESHAFSTYDKFL 240

Query: 241 KAEGEELKKQPAPEVAVKYYTEGDLYLFDEFQTSRAPKSRRPKIESLYDVFVNIRDDEAE 300
           KA+GEELKK PAPEVAVKYYTEGDLYLFDEFQTSRAP SRRPKIE+LYDVFVNIRDDEAE
Sbjct: 241 KAQGEELKKLPAPEVAVKYYTEGDLYLFDEFQTSRAPNSRRPKIENLYDVFVNIRDDEAE 300

Query: 301 HCKTMRACQSHGNLRSPHSYSESCPEDDEPLCNLAE-ADCEGIVDCIKKSITSSPPVKQK 356
           HCKTMRACQSHGNLRSPHSYSES PEDDEPLCNL E ADCEGIVDC+KK++TSSPPVK K
Sbjct: 301 HCKTMRACQSHGNLRSPHSYSESRPEDDEPLCNLPEAADCEGIVDCLKKAVTSSPPVKPK 360

BLAST of Cmc12g0325721 vs. TAIR 10
Match: AT4G22260.1 (Alternative oxidase family protein )

HSP 1 Score: 459.1 bits (1180), Expect = 3.1e-129
Identity = 230/311 (73.95%), Postives = 257/311 (82.64%), Query Frame = 0

Query: 38  SARPLISRKSCRVMATVLKEDEEKVVLEESFPPKASPVQDGEGGSGQGPQSSSSNGLDKW 97
           S+R L+ R + RV AT+L++DEEKVV+EESF  KA      E        SSS++  + W
Sbjct: 43  SSRRLLLRNNHRVQATILQDDEEKVVVEESF--KAETSTGTEPLEEPNMSSSSTSAFETW 102

Query: 98  IIKLEQSINVLLTDSVIKILDAFYHDRHYARFFVLETIARVPYFAFVSVLHMYESFGWWR 157
           IIKLEQ +NV LTDSVIKILD  Y DR YARFFVLETIARVPYFAF+SVLHMYE+FGWWR
Sbjct: 103 IIKLEQGVNVFLTDSVIKILDTLYRDRTYARFFVLETIARVPYFAFMSVLHMYETFGWWR 162

Query: 158 RADYLKVHFAESWNEMHHLLIMEELGGNDWWFDRFLAQHIAVAYYFMTVFMYMISPRMAY 217
           RADYLKVHFAESWNEMHHLLIMEELGGN WWFDRFLAQHIA  YYFMTVF+Y++SPRMAY
Sbjct: 163 RADYLKVHFAESWNEMHHLLIMEELGGNSWWFDRFLAQHIATFYYFMTVFLYILSPRMAY 222

Query: 218 HFSECVESHAFSTYDKFLKAEGEELKKQPAPEVAVKYYTEGDLYLFDEFQTSRAPKSRRP 277
           HFSECVESHA+ TYDKFLKA GEELK  PAP++AVKYYT GDLYLFDEFQTSR P +RRP
Sbjct: 223 HFSECVESHAYETYDKFLKASGEELKNMPAPDIAVKYYTGGDLYLFDEFQTSRTPNTRRP 282

Query: 278 KIESLYDVFVNIRDDEAEHCKTMRACQSHGNLRSPHSYSESCPEDDEPLCNL-AEADCEG 337
            IE+LYDVFVNIRDDEAEHCKTMRACQ+ G+LRSPHS  E    ++E  C +  EA CEG
Sbjct: 283 VIENLYDVFVNIRDDEAEHCKTMRACQTLGSLRSPHSILEDDDTEEESGCVVPEEAHCEG 342

Query: 338 IVDCIKKSITS 348
           IVDC+KKSITS
Sbjct: 343 IVDCLKKSITS 351

BLAST of Cmc12g0325721 vs. TAIR 10
Match: AT1G32350.1 (alternative oxidase 1D )

HSP 1 Score: 67.4 bits (163), Expect = 2.6e-11
Identity = 51/189 (26.98%), Postives = 86/189 (45.50%), Query Frame = 0

Query: 110 TDSVIKI-LDAFYHDRHYARFFVLETIARVPYFAFVSVLHMYESFGWWRRADYLKVHFAE 169
           T   +KI +  F+  +H     +LET+A VP      +LH+     +     ++K    E
Sbjct: 123 TVQTLKIPVQLFFQRKHMCHAMLLETVAAVPGMVGGMLLHLKSLRRFEHSGGWIKALLEE 182

Query: 170 SWNEMHHLLIMEELGGNDWWFDRFLAQHIAVAYYFMTVFMYMISPRMAYHFSECVESHAF 229
           + NE  HL+   EL     W++R +   +   ++      Y+ISP++A+  +  +E  A 
Sbjct: 183 AENERMHLMTFIEL-SQPKWYERAIVFTVQGVFFNAYFLAYVISPKLAHRITGYLEEEAV 242

Query: 230 STYDKFLK-AEGEELKKQPAPEVAVKYYTEGDLYLFDEFQTSRAPKSRRPKIESLYDVFV 289
           ++Y +FLK  +  + +  PAP +A+ Y+                   R PK  +L DV  
Sbjct: 243 NSYTEFLKDIDAGKFENSPAPAIAIDYW-------------------RLPKDATLRDVVY 291

Query: 290 NIRDDEAEH 297
            IR DEA H
Sbjct: 303 VIRADEAHH 291

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_008458986.11.1e-200100.00PREDICTED: ubiquinol oxidase 4, chloroplastic/chromoplastic-like [Cucumis melo] ... [more]
XP_004145426.11.1e-19596.34ubiquinol oxidase 4, chloroplastic/chromoplastic [Cucumis sativus][more]
XP_038893742.19.9e-18691.39ubiquinol oxidase 4, chloroplastic/chromoplastic-like [Benincasa hispida][more]
XP_022994224.12.9e-17787.26ubiquinol oxidase 4, chloroplastic/chromoplastic [Cucurbita maxima][more]
XP_022955227.11.4e-17687.53ubiquinol oxidase 4, chloroplastic/chromoplastic [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
Q56X524.4e-12873.95Ubiquinol oxidase 4, chloroplastic/chromoplastic OS=Arabidopsis thaliana OX=3702... [more]
Q8LEE73.7e-1026.98Ubiquinol oxidase 3, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=AOX3 PE=1 ... [more]
Match NameE-valueIdentityDescription
A0A5A7UI875.3e-201100.00Ubiquinol oxidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold302G00... [more]
A0A1S3C9925.3e-201100.00Ubiquinol oxidase OS=Cucumis melo OX=3656 GN=LOC103498237 PE=3 SV=1[more]
A0A0A0M1305.1e-19696.34Ubiquinol oxidase OS=Cucumis sativus OX=3659 GN=Csa_1G699610 PE=3 SV=1[more]
A0A6J1K2B21.4e-17787.26Ubiquinol oxidase OS=Cucurbita maxima OX=3661 GN=LOC111490026 PE=3 SV=1[more]
A0A6J1GSZ77.0e-17787.53Ubiquinol oxidase OS=Cucurbita moschata OX=3662 GN=LOC111457254 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT4G22260.13.1e-12973.95Alternative oxidase family protein [more]
AT1G32350.12.6e-1126.98alternative oxidase 1D [more]
InterPro
Analysis Name: InterPro Annotations of Melon (Charmono) v1.1
Date Performed: 2022-10-13
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR038659Alternative oxidase superfamilyGENE3D1.20.1260.140Alternative oxidasecoord: 84..313
e-value: 4.9E-60
score: 204.8
IPR002680Alternative oxidasePFAMPF01786AOXcoord: 110..303
e-value: 1.4E-37
score: 129.4
IPR002680Alternative oxidasePANTHERPTHR31803ALTERNATIVE OXIDASEcoord: 33..341
IPR002680Alternative oxidaseCDDcd01053AOXcoord: 121..307
e-value: 1.00862E-96
score: 282.559
NoneNo IPR availablePIRSRPIRSR005229-1PIRSR005229-1coord: 123..298
e-value: 3.8E-13
score: 47.2
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 70..91
NoneNo IPR availablePANTHERPTHR31803:SF10UBIQUINOL OXIDASE 4, CHLOROPLASTIC/CHROMOPLASTICcoord: 33..341

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cmc12g0325721.1Cmc12g0325721.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0009916 alternative oxidase activity