Cmc12g0321571 (gene) Melon (Charmono) v1.1

Overview


Name	Cmc12g0321571
Type	gene
Organism	Cucumis melo L. var. cantalupensis cv. Charmono (Melon (Charmono) v1.1)
Description	aspartic proteinase nepenthesin-1
Location	CMiso1.1chr12: 8637775 .. 8638433 (-)
RNA-Seq Expression	Cmc12g0321571
Synteny	Cmc12g0321571

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAAAGCAACCCCATTGATCAAAAACCCTTCTCAACCATCTTTTTACTATCTTTCTCTCCAAGGAATCTCAGTTGGTGGAACTCAGTTATCAATACCAAAGTCCACTTTTGAGCTCCATGATGATGGGAGTGGAGGAGTAATCATAGACTCAGGCACAACAATCACTTACATTGAGAGCACATCTTTCTCTTCACTCAAAAATGAGTTAATTGCTCAAATGAATCTTCCCGTTGACGATTCTGGTACTGGTGGTCTTGACCTCTGCTTTAACCTACCAGCAGGGACAACTCAGGTACGTCCACTTTGATATGTTACAATACACAACAATATATTAATACGAGTATAATCACTGTTCCCATGTTTAATATATATGTTTCCAAAACACAACTTGATCATGTCATTGATCGTTTTGTTTACGTCTTTTTAACAAAGGTGGAGGTTCCGAAGTTGACGTTTCATTTCAAGGGCGCGGATTTGGAGCTGCCTGGGGAGAATTACATGATTGGGGATTCAAAGACGGGATTGCTATGCTTGGCCATTGGGAGTTCTAGAGGAATGTCCATCTTTGGAAATCTACAGCAACAAAATTTCATGGTTGTTCACGATCTTCAAGAAGAAACCCTATCTTTTTTGCCAACTCAATGTGATAGTATATAA

mRNA sequence

ATGAAAGCAACCCCATTGATCAAAAACCCTTCTCAACCATCTTTTTACTATCTTTCTCTCCAAGGAATCTCAGTTGGTGGAACTCAGTTATCAATACCAAAGTCCACTTTTGAGCTCCATGATGATGGGAGTGGAGGAGTAATCATAGACTCAGGCACAACAATCACTTACATTGAGAGCACATCTTTCTCTTCACTCAAAAATGAGTTAATTGCTCAAATGAATCTTCCCGTTGACGATTCTGGTACTGGTGGTCTTGACCTCTGCTTTAACCTACCAGCAGGGACAACTCAGGTGGAGGTTCCGAAGTTGACGTTTCATTTCAAGGGCGCGGATTTGGAGCTGCCTGGGGAGAATTACATGATTGGGGATTCAAAGACGGGATTGCTATGCTTGGCCATTGGGAGTTCTAGAGGAATGTCCATCTTTGGAAATCTACAGCAACAAAATTTCATGGTTGTTCACGATCTTCAAGAAGAAACCCTATCTTTTTTGCCAACTCAATGTGATAGTATATAA

Coding sequence (CDS)

ATGAAAGCAACCCCATTGATCAAAAACCCTTCTCAACCATCTTTTTACTATCTTTCTCTCCAAGGAATCTCAGTTGGTGGAACTCAGTTATCAATACCAAAGTCCACTTTTGAGCTCCATGATGATGGGAGTGGAGGAGTAATCATAGACTCAGGCACAACAATCACTTACATTGAGAGCACATCTTTCTCTTCACTCAAAAATGAGTTAATTGCTCAAATGAATCTTCCCGTTGACGATTCTGGTACTGGTGGTCTTGACCTCTGCTTTAACCTACCAGCAGGGACAACTCAGGTGGAGGTTCCGAAGTTGACGTTTCATTTCAAGGGCGCGGATTTGGAGCTGCCTGGGGAGAATTACATGATTGGGGATTCAAAGACGGGATTGCTATGCTTGGCCATTGGGAGTTCTAGAGGAATGTCCATCTTTGGAAATCTACAGCAACAAAATTTCATGGTTGTTCACGATCTTCAAGAAGAAACCCTATCTTTTTTGCCAACTCAATGTGATAGTATATAA

Protein sequence

MKATPLIKNPSQPSFYYLSLQGISVGGTQLSIPKSTFELHDDGSGGVIIDSGTTITYIESTSFSSLKNELIAQMNLPVDDSGTGGLDLCFNLPAGTTQVEVPKLTFHFKGADLELPGENYMIGDSKTGLLCLAIGSSRGMSIFGNLQQQNFMVVHDLQEETLSFLPTQCDSI

Homology

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BLAST of Cmc12g0321571 vs. NCBI nr
Match: TYK02448.1 (aspartic proteinase nepenthesin-1 [Cucumis melo var. makuwa])

HSP 1 Score: 347.1 bits (889), Expect = 9.0e-92
Identity = 170/172 (98.84%), Postives = 171/172 (99.42%), Query Frame = 0


Query: 1   MKATPLIKNPSQPSFYYLSLQGISVGGTQLSIPKSTFELHDDGSGGVIIDSGTTITYIES 60
           MKATPLIKNPSQPSFYYLSLQGISVGGTQLSIPKSTFELHDDGSGGVIIDSGTTITYIES
Sbjct: 290 MKATPLIKNPSQPSFYYLSLQGISVGGTQLSIPKSTFELHDDGSGGVIIDSGTTITYIES 349

Query: 61  TSFSSLKNELIAQMNLPVDDSGTGGLDLCFNLPAGTTQVEVPKLTFHFKGADLELPGENY 120
           T+FSSLKNE IAQMNLPVDDSGTGGLDLCFNLPAGTTQVEVPKLTFHFKGADLELPGENY
Sbjct: 350 TAFSSLKNEFIAQMNLPVDDSGTGGLDLCFNLPAGTTQVEVPKLTFHFKGADLELPGENY 409

Query: 121 MIGDSKTGLLCLAIGSSRGMSIFGNLQQQNFMVVHDLQEETLSFLPTQCDSI 173
           MIGDSKTGLLCLAIGSSRGMSIFGNLQQQNFMVVHDLQEETLSFLPTQCDSI
Sbjct: 410 MIGDSKTGLLCLAIGSSRGMSIFGNLQQQNFMVVHDLQEETLSFLPTQCDSI 461

[Close]

BLAST of Cmc12g0321571 vs. NCBI nr
Match: XP_008442220.1 (PREDICTED: aspartic proteinase nepenthesin-1 [Cucumis melo] >KAA0041170.1 aspartic proteinase nepenthesin-1 [Cucumis melo var. makuwa])

HSP 1 Score: 347.1 bits (889), Expect = 9.0e-92
Identity = 170/172 (98.84%), Postives = 171/172 (99.42%), Query Frame = 0


Query: 1   MKATPLIKNPSQPSFYYLSLQGISVGGTQLSIPKSTFELHDDGSGGVIIDSGTTITYIES 60
           MKATPLIKNPSQPSFYYLSLQGISVGGTQLSIPKSTFELHDDGSGGVIIDSGTTITYIES
Sbjct: 290 MKATPLIKNPSQPSFYYLSLQGISVGGTQLSIPKSTFELHDDGSGGVIIDSGTTITYIES 349

Query: 61  TSFSSLKNELIAQMNLPVDDSGTGGLDLCFNLPAGTTQVEVPKLTFHFKGADLELPGENY 120
           T+FSSLKNE IAQMNLPVDDSGTGGLDLCFNLPAGTTQVEVPKLTFHFKGADLELPGENY
Sbjct: 350 TAFSSLKNEFIAQMNLPVDDSGTGGLDLCFNLPAGTTQVEVPKLTFHFKGADLELPGENY 409

Query: 121 MIGDSKTGLLCLAIGSSRGMSIFGNLQQQNFMVVHDLQEETLSFLPTQCDSI 173
           MIGDSKTGLLCLAIGSSRGMSIFGNLQQQNFMVVHDLQEETLSFLPTQCDSI
Sbjct: 410 MIGDSKTGLLCLAIGSSRGMSIFGNLQQQNFMVVHDLQEETLSFLPTQCDSI 461

[Close]

BLAST of Cmc12g0321571 vs. NCBI nr
Match: XP_011653928.1 (aspartic proteinase nepenthesin-1 [Cucumis sativus] >KGN54860.1 hypothetical protein Csa_012038 [Cucumis sativus])

HSP 1 Score: 337.4 bits (864), Expect = 7.1e-89
Identity = 163/172 (94.77%), Postives = 168/172 (97.67%), Query Frame = 0


Query: 1   MKATPLIKNPSQPSFYYLSLQGISVGGTQLSIPKSTFELHDDGSGGVIIDSGTTITYIES 60
           MK TPLIKNPSQPSFYYLSLQGISVGGTQLSIPKSTFELHDDGSGGVIIDSGTTITY+E+
Sbjct: 290 MKTTPLIKNPSQPSFYYLSLQGISVGGTQLSIPKSTFELHDDGSGGVIIDSGTTITYVEN 349

Query: 61  TSFSSLKNELIAQMNLPVDDSGTGGLDLCFNLPAGTTQVEVPKLTFHFKGADLELPGENY 120
           ++F+SLKNE IAQMNLPVDDSGTGGLDLCFNLPAGT QVEVPKLTFHFKGADLELPGENY
Sbjct: 350 SAFTSLKNEFIAQMNLPVDDSGTGGLDLCFNLPAGTNQVEVPKLTFHFKGADLELPGENY 409

Query: 121 MIGDSKTGLLCLAIGSSRGMSIFGNLQQQNFMVVHDLQEETLSFLPTQCDSI 173
           MIGDSK GLLCLAIGSSRGMSIFGNLQQQNFMVVHDLQEETLSFLPTQCDSI
Sbjct: 410 MIGDSKAGLLCLAIGSSRGMSIFGNLQQQNFMVVHDLQEETLSFLPTQCDSI 461

[Close]

BLAST of Cmc12g0321571 vs. NCBI nr
Match: XP_038883313.1 (aspartic proteinase nepenthesin-1 [Benincasa hispida])

HSP 1 Score: 327.8 bits (839), Expect = 5.7e-86
Identity = 158/172 (91.86%), Postives = 166/172 (96.51%), Query Frame = 0


Query: 1   MKATPLIKNPSQPSFYYLSLQGISVGGTQLSIPKSTFELHDDGSGGVIIDSGTTITYIES 60
           MK TPLI+NPSQPSFYYLSLQGISVG TQLSIPK+TFELHDDGSGGVIIDSGTTITYIE+
Sbjct: 283 MKTTPLIRNPSQPSFYYLSLQGISVGSTQLSIPKTTFELHDDGSGGVIIDSGTTITYIEN 342

Query: 61  TSFSSLKNELIAQMNLPVDDSGTGGLDLCFNLPAGTTQVEVPKLTFHFKGADLELPGENY 120
           T+F+ LKNE IAQMNLPVDDSGTGGLDLCFNLPA  TQVEVPKLTFHF+GADLELPGENY
Sbjct: 343 TAFTLLKNEFIAQMNLPVDDSGTGGLDLCFNLPAEATQVEVPKLTFHFEGADLELPGENY 402

Query: 121 MIGDSKTGLLCLAIGSSRGMSIFGNLQQQNFMVVHDLQEETLSFLPTQCDSI 173
           MIGDS+TGL+CLAIGSSRGMSIFGNLQQQNFMVVHDLQEETLSFLPTQCDSI
Sbjct: 403 MIGDSRTGLICLAIGSSRGMSIFGNLQQQNFMVVHDLQEETLSFLPTQCDSI 454

[Close]

BLAST of Cmc12g0321571 vs. NCBI nr
Match: XP_022928703.1 (aspartic proteinase nepenthesin-1 [Cucurbita moschata])

HSP 1 Score: 303.9 bits (777), Expect = 8.7e-79
Identity = 146/172 (84.88%), Postives = 155/172 (90.12%), Query Frame = 0


Query: 1   MKATPLIKNPSQPSFYYLSLQGISVGGTQLSIPKSTFELHDDGSGGVIIDSGTTITYIES 60
           +K TPLI+NPSQPSFYYLSLQGISVGGTQL IPK+TFELHDDGSGGVIIDSGTTITYIE 
Sbjct: 283 IKTTPLIRNPSQPSFYYLSLQGISVGGTQLPIPKATFELHDDGSGGVIIDSGTTITYIEK 342

Query: 61  TSFSSLKNELIAQMNLPVDDSGTGGLDLCFNLPAGTTQVEVPKLTFHFKGADLELPGENY 120
            +F+ LK E ++QM LPVDDSGT GLDLCFNLP  TTQVEVPKLTFHFKGADLELPGENY
Sbjct: 343 NAFTLLKKEFVSQMKLPVDDSGTSGLDLCFNLPPETTQVEVPKLTFHFKGADLELPGENY 402

Query: 121 MIGDSKTGLLCLAIGSSRGMSIFGNLQQQNFMVVHDLQEETLSFLPTQCDSI 173
           MIGDSK  L+CLAIGSS GMSIFGNLQQQN MVVHDLQEET+SFLPTQC  I
Sbjct: 403 MIGDSKAELICLAIGSSSGMSIFGNLQQQNIMVVHDLQEETVSFLPTQCSEI 454

[Close]

BLAST of Cmc12g0321571 vs. ExPASy Swiss-Prot
Match: Q766C3 (Aspartic proteinase nepenthesin-1 OS=Nepenthes gracilis OX=150966 GN=nep1 PE=1 SV=1)

HSP 1 Score: 175.6 bits (444), Expect = 4.7e-43
Identity = 84/168 (50.00%), Postives = 118/168 (70.24%), Query Frame = 0


Query: 4   TPLIKNPSQPSFYYLSLQGISVGGTQLSIPKSTFELH-DDGSGGVIIDSGTTITYIESTS 63
           T LI++   P+FYY++L G+SVG T+L I  S F L+ ++G+GG+IIDSGTT+TY  + +
Sbjct: 268 TTLIQSSQIPTFYYITLNGLSVGSTRLPIDPSAFALNSNNGTGGIIIDSGTTLTYFVNNA 327

Query: 64  FSSLKNELIAQMNLPVDDSGTGGLDLCFNLPAGTTQVEVPKLTFHFKGADLELPGENYMI 123
           + S++ E I+Q+NLPV +  + G DLCF  P+  + +++P    HF G DLELP ENY I
Sbjct: 328 YQSVRQEFISQINLPVVNGSSSGFDLCFQTPSDPSNLQIPTFVMHFDGGDLELPSENYFI 387

Query: 124 GDSKTGLLCLAIG-SSRGMSIFGNLQQQNFMVVHDLQEETLSFLPTQC 170
             S  GL+CLA+G SS+GMSIFGN+QQQN +VV+D     +SF   QC
Sbjct: 388 SPS-NGLICLAMGSSSQGMSIFGNIQQQNMLVVYDTGNSVVSFASAQC 434

[Close]

BLAST of Cmc12g0321571 vs. ExPASy Swiss-Prot
Match: Q766C2 (Aspartic proteinase nepenthesin-2 OS=Nepenthes gracilis OX=150966 GN=nep2 PE=1 SV=1)

HSP 1 Score: 166.4 bits (420), Expect = 2.9e-40
Identity = 79/169 (46.75%), Postives = 117/169 (69.23%), Query Frame = 0


Query: 3   ATPLIKNPSQPSFYYLSLQGISVGGTQLSIPKSTFELHDDGSGGVIIDSGTTITYIESTS 62
           +T LI +   P++YY++LQGI+VGG  L IP STF+L DDG+GG+IIDSGTT+TY+   +
Sbjct: 268 STTLIHSSLNPTYYYITLQGITVGGDNLGIPSSTFQLQDDGTGGMIIDSGTTLTYLPQDA 327

Query: 63  FSSLKNELIAQMNLPVDDSGTGGLDLCFNLPAGTTQVEVPKLTFHFKGADLELPGENYMI 122
           ++++      Q+NLP  D  + GL  CF  P+  + V+VP+++  F G  L L  +N +I
Sbjct: 328 YNAVAQAFTDQINLPTVDESSSGLSTCFQQPSDGSTVQVPEISMQFDGGVLNLGEQNILI 387

Query: 123 GDSKTGLLCLAIGSSR--GMSIFGNLQQQNFMVVHDLQEETLSFLPTQC 170
             ++ G++CLA+GSS   G+SIFGN+QQQ   V++DLQ   +SF+PTQC
Sbjct: 388 SPAE-GVICLAMGSSSQLGISIFGNIQQQETQVLYDLQNLAVSFVPTQC 435

[Close]

BLAST of Cmc12g0321571 vs. ExPASy Swiss-Prot
Match: Q9LNJ3 (Aspartyl protease family protein 2 OS=Arabidopsis thaliana OX=3702 GN=APF2 PE=2 SV=1)

HSP 1 Score: 126.7 bits (317), Expect = 2.5e-28
Identity = 67/168 (39.88%), Postives = 97/168 (57.74%), Query Frame = 0


Query: 4   TPLIKNPSQPSFYYLSLQGISVGGTQL-SIPKSTFELHDDGSGGVIIDSGTTITYIESTS 63
           TPL+ NP   +FYY+ L GISVGGT++  +  S F+L   G+GGVIIDSGT++T +   +
Sbjct: 318 TPLLSNPKLDTFYYVGLLGISVGGTRVPGVTASLFKLDQIGNGGVIIDSGTSVTRLIRPA 377

Query: 64  FSSLKNELIAQMNLPVDDSGTGGLDLCFNLPAGTTQVEVPKLTFHFKGADLELPGENYMI 123
           + ++++                  D CF+L +   +V+VP +  HF+GAD+ LP  NY+I
Sbjct: 378 YIAMRDAFRVGAKTLKRAPDFSLFDTCFDL-SNMNEVKVPTVVLHFRGADVSLPATNYLI 437

Query: 124 GDSKTGLLCLAI-GSSRGMSIFGNLQQQNFMVVHDLQEETLSFLPTQC 170
                G  C A  G+  G+SI GN+QQQ F VV+DL    + F P  C
Sbjct: 438 PVDTNGKFCFAFAGTMGGLSIIGNIQQQGFRVVYDLASSRVGFAPGGC 484

[Close]

BLAST of Cmc12g0321571 vs. ExPASy Swiss-Prot
Match: Q6XBF8 (Aspartic proteinase CDR1 OS=Arabidopsis thaliana OX=3702 GN=CDR1 PE=1 SV=1)

HSP 1 Score: 118.6 bits (296), Expect = 6.8e-26
Identity = 64/167 (38.32%), Postives = 96/167 (57.49%), Query Frame = 0


Query: 3   ATPLIKNPSQPSFYYLSLQGISVGGTQLSIPKSTFELHDDGSGGVIIDSGTTITYIESTS 62
           +TPLI   SQ +FYYL+L+ ISVG  Q+    S  E      G +IIDSGTT+T + +  
Sbjct: 275 STPLIAKASQETFYYLTLKSISVGSKQIQYSGSDSE---SSEGNIIIDSGTTLTLLPTEF 334

Query: 63  FSSLKNELIAQMNLPVDDSGTGGLDLCFNLPAGTTQVEVPKLTFHFKGADLELPGENYMI 122
           +S L++ + + ++         GL LC+   + T  ++VP +T HF GAD++L   N  +
Sbjct: 335 YSELEDAVASSIDAEKKQDPQSGLSLCY---SATGDLKVPVITMHFDGADVKLDSSNAFV 394

Query: 123 GDSKTGLLCLAIGSSRGMSIFGNLQQQNFMVVHDLQEETLSFLPTQC 170
             S+  L+C A   S   SI+GN+ Q NF+V +D   +T+SF PT C
Sbjct: 395 QVSE-DLVCFAFRGSPSFSIYGNVAQMNFLVGYDTVSKTVSFKPTDC 434

[Close]

BLAST of Cmc12g0321571 vs. ExPASy Swiss-Prot
Match: Q9LS40 (Protein ASPARTIC PROTEASE IN GUARD CELL 1 OS=Arabidopsis thaliana OX=3702 GN=ASPG1 PE=1 SV=1)

HSP 1 Score: 117.5 bits (293), Expect = 1.5e-25
Identity = 59/168 (35.12%), Postives = 99/168 (58.93%), Query Frame = 0


Query: 5   PLIKNPSQPSFYYLSLQGISVGGTQLSIPKSTFELHDDGSGGVIIDSGTTITYIESTSFS 64
           PL++N    +FYY+ L G SVGG ++ +P + F++   GSGGVI+D GT +T +++ +++
Sbjct: 334 PLLRNKKIDTFYYVGLSGFSVGGEKVVLPDAIFDVDASGSGGVILDCGTAVTRLQTQAYN 393

Query: 65  SLKNELI-AQMNLPVDDSGTGGLDLCFNLPAGTTQVEVPKLTFHFKGA-DLELPGENYMI 124
           SL++  +   +NL    S     D C++  + +T V+VP + FHF G   L+LP +NY+I
Sbjct: 394 SLRDAFLKLTVNLKKGSSSISLFDTCYDFSSLST-VKVPTVAFHFTGGKSLDLPAKNYLI 453

Query: 125 GDSKTGLLCLAIG-SSRGMSIFGNLQQQNFMVVHDLQEETLSFLPTQC 170
               +G  C A   +S  +SI GN+QQQ   + +DL +  +     +C
Sbjct: 454 PVDDSGTFCFAFAPTSSSLSIIGNVQQQGTRITYDLSKNVIGLSGNKC 500

[Close]

BLAST of Cmc12g0321571 vs. ExPASy TrEMBL
Match: A0A5A7TD10 (Aspartic proteinase nepenthesin-1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold128G001020 PE=3 SV=1)

HSP 1 Score: 347.1 bits (889), Expect = 4.4e-92
Identity = 170/172 (98.84%), Postives = 171/172 (99.42%), Query Frame = 0


Query: 1   MKATPLIKNPSQPSFYYLSLQGISVGGTQLSIPKSTFELHDDGSGGVIIDSGTTITYIES 60
           MKATPLIKNPSQPSFYYLSLQGISVGGTQLSIPKSTFELHDDGSGGVIIDSGTTITYIES
Sbjct: 290 MKATPLIKNPSQPSFYYLSLQGISVGGTQLSIPKSTFELHDDGSGGVIIDSGTTITYIES 349

Query: 61  TSFSSLKNELIAQMNLPVDDSGTGGLDLCFNLPAGTTQVEVPKLTFHFKGADLELPGENY 120
           T+FSSLKNE IAQMNLPVDDSGTGGLDLCFNLPAGTTQVEVPKLTFHFKGADLELPGENY
Sbjct: 350 TAFSSLKNEFIAQMNLPVDDSGTGGLDLCFNLPAGTTQVEVPKLTFHFKGADLELPGENY 409

Query: 121 MIGDSKTGLLCLAIGSSRGMSIFGNLQQQNFMVVHDLQEETLSFLPTQCDSI 173
           MIGDSKTGLLCLAIGSSRGMSIFGNLQQQNFMVVHDLQEETLSFLPTQCDSI
Sbjct: 410 MIGDSKTGLLCLAIGSSRGMSIFGNLQQQNFMVVHDLQEETLSFLPTQCDSI 461

[Close]

BLAST of Cmc12g0321571 vs. ExPASy TrEMBL
Match: A0A5D3BTY9 (Aspartic proteinase nepenthesin-1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1738G00580 PE=3 SV=1)

HSP 1 Score: 347.1 bits (889), Expect = 4.4e-92
Identity = 170/172 (98.84%), Postives = 171/172 (99.42%), Query Frame = 0


Query: 1   MKATPLIKNPSQPSFYYLSLQGISVGGTQLSIPKSTFELHDDGSGGVIIDSGTTITYIES 60
           MKATPLIKNPSQPSFYYLSLQGISVGGTQLSIPKSTFELHDDGSGGVIIDSGTTITYIES
Sbjct: 290 MKATPLIKNPSQPSFYYLSLQGISVGGTQLSIPKSTFELHDDGSGGVIIDSGTTITYIES 349

Query: 61  TSFSSLKNELIAQMNLPVDDSGTGGLDLCFNLPAGTTQVEVPKLTFHFKGADLELPGENY 120
           T+FSSLKNE IAQMNLPVDDSGTGGLDLCFNLPAGTTQVEVPKLTFHFKGADLELPGENY
Sbjct: 350 TAFSSLKNEFIAQMNLPVDDSGTGGLDLCFNLPAGTTQVEVPKLTFHFKGADLELPGENY 409

Query: 121 MIGDSKTGLLCLAIGSSRGMSIFGNLQQQNFMVVHDLQEETLSFLPTQCDSI 173
           MIGDSKTGLLCLAIGSSRGMSIFGNLQQQNFMVVHDLQEETLSFLPTQCDSI
Sbjct: 410 MIGDSKTGLLCLAIGSSRGMSIFGNLQQQNFMVVHDLQEETLSFLPTQCDSI 461

[Close]

BLAST of Cmc12g0321571 vs. ExPASy TrEMBL
Match: A0A1S3B573 (aspartic proteinase nepenthesin-1 OS=Cucumis melo OX=3656 GN=LOC103486136 PE=3 SV=1)

HSP 1 Score: 347.1 bits (889), Expect = 4.4e-92
Identity = 170/172 (98.84%), Postives = 171/172 (99.42%), Query Frame = 0


Query: 1   MKATPLIKNPSQPSFYYLSLQGISVGGTQLSIPKSTFELHDDGSGGVIIDSGTTITYIES 60
           MKATPLIKNPSQPSFYYLSLQGISVGGTQLSIPKSTFELHDDGSGGVIIDSGTTITYIES
Sbjct: 290 MKATPLIKNPSQPSFYYLSLQGISVGGTQLSIPKSTFELHDDGSGGVIIDSGTTITYIES 349

Query: 61  TSFSSLKNELIAQMNLPVDDSGTGGLDLCFNLPAGTTQVEVPKLTFHFKGADLELPGENY 120
           T+FSSLKNE IAQMNLPVDDSGTGGLDLCFNLPAGTTQVEVPKLTFHFKGADLELPGENY
Sbjct: 350 TAFSSLKNEFIAQMNLPVDDSGTGGLDLCFNLPAGTTQVEVPKLTFHFKGADLELPGENY 409

Query: 121 MIGDSKTGLLCLAIGSSRGMSIFGNLQQQNFMVVHDLQEETLSFLPTQCDSI 173
           MIGDSKTGLLCLAIGSSRGMSIFGNLQQQNFMVVHDLQEETLSFLPTQCDSI
Sbjct: 410 MIGDSKTGLLCLAIGSSRGMSIFGNLQQQNFMVVHDLQEETLSFLPTQCDSI 461

[Close]

BLAST of Cmc12g0321571 vs. ExPASy TrEMBL
Match: A0A0A0KYT9 (Peptidase A1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G554680 PE=3 SV=1)

HSP 1 Score: 337.4 bits (864), Expect = 3.5e-89
Identity = 163/172 (94.77%), Postives = 168/172 (97.67%), Query Frame = 0


Query: 1   MKATPLIKNPSQPSFYYLSLQGISVGGTQLSIPKSTFELHDDGSGGVIIDSGTTITYIES 60
           MK TPLIKNPSQPSFYYLSLQGISVGGTQLSIPKSTFELHDDGSGGVIIDSGTTITY+E+
Sbjct: 290 MKTTPLIKNPSQPSFYYLSLQGISVGGTQLSIPKSTFELHDDGSGGVIIDSGTTITYVEN 349

Query: 61  TSFSSLKNELIAQMNLPVDDSGTGGLDLCFNLPAGTTQVEVPKLTFHFKGADLELPGENY 120
           ++F+SLKNE IAQMNLPVDDSGTGGLDLCFNLPAGT QVEVPKLTFHFKGADLELPGENY
Sbjct: 350 SAFTSLKNEFIAQMNLPVDDSGTGGLDLCFNLPAGTNQVEVPKLTFHFKGADLELPGENY 409

Query: 121 MIGDSKTGLLCLAIGSSRGMSIFGNLQQQNFMVVHDLQEETLSFLPTQCDSI 173
           MIGDSK GLLCLAIGSSRGMSIFGNLQQQNFMVVHDLQEETLSFLPTQCDSI
Sbjct: 410 MIGDSKAGLLCLAIGSSRGMSIFGNLQQQNFMVVHDLQEETLSFLPTQCDSI 461

[Close]

BLAST of Cmc12g0321571 vs. ExPASy TrEMBL
Match: A0A6J1EPU6 (aspartic proteinase nepenthesin-1 OS=Cucurbita moschata OX=3662 GN=LOC111435534 PE=3 SV=1)

HSP 1 Score: 303.9 bits (777), Expect = 4.2e-79
Identity = 146/172 (84.88%), Postives = 155/172 (90.12%), Query Frame = 0


Query: 1   MKATPLIKNPSQPSFYYLSLQGISVGGTQLSIPKSTFELHDDGSGGVIIDSGTTITYIES 60
           +K TPLI+NPSQPSFYYLSLQGISVGGTQL IPK+TFELHDDGSGGVIIDSGTTITYIE 
Sbjct: 283 IKTTPLIRNPSQPSFYYLSLQGISVGGTQLPIPKATFELHDDGSGGVIIDSGTTITYIEK 342

Query: 61  TSFSSLKNELIAQMNLPVDDSGTGGLDLCFNLPAGTTQVEVPKLTFHFKGADLELPGENY 120
            +F+ LK E ++QM LPVDDSGT GLDLCFNLP  TTQVEVPKLTFHFKGADLELPGENY
Sbjct: 343 NAFTLLKKEFVSQMKLPVDDSGTSGLDLCFNLPPETTQVEVPKLTFHFKGADLELPGENY 402

Query: 121 MIGDSKTGLLCLAIGSSRGMSIFGNLQQQNFMVVHDLQEETLSFLPTQCDSI 173
           MIGDSK  L+CLAIGSS GMSIFGNLQQQN MVVHDLQEET+SFLPTQC  I
Sbjct: 403 MIGDSKAELICLAIGSSSGMSIFGNLQQQNIMVVHDLQEETVSFLPTQCSEI 454

[Close]

BLAST of Cmc12g0321571 vs. TAIR 10
Match: AT2G03200.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 251.1 bits (640), Expect = 6.3e-67
Identity = 114/171 (66.67%), Postives = 141/171 (82.46%), Query Frame = 0


Query: 2   KATPLIKNPSQPSFYYLSLQGISVGGTQLSIPKSTFELHDDGSGGVIIDSGTTITYIEST 61
           K   L++NP QPSFYYL LQGI+VG  +LS+ KSTFEL +DG+GG+IIDSGTTITY+E T
Sbjct: 291 KTMSLLRNPDQPSFYYLELQGITVGAKRLSVEKSTFELAEDGTGGMIIDSGTTITYLEET 350

Query: 62  SFSSLKNELIAQMNLPVDDSGTGGLDLCFNLPAGTTQVEVPKLTFHFKGADLELPGENYM 121
           +F  LK E  ++M+LPVDDSG+ GLDLCF LP     + VPK+ FHFKGADLELPGENYM
Sbjct: 351 AFKVLKEEFTSRMSLPVDDSGSTGLDLCFKLPDAAKNIAVPKMIFHFKGADLELPGENYM 410

Query: 122 IGDSKTGLLCLAIGSSRGMSIFGNLQQQNFMVVHDLQEETLSFLPTQCDSI 173
           + DS TG+LCLA+GSS GMSIFGN+QQQNF V+HDL++ET+SF+PT+C  +
Sbjct: 411 VADSSTGVLCLAMGSSNGMSIFGNVQQQNFNVLHDLEKETVSFVPTECGKL 461

[Close]

BLAST of Cmc12g0321571 vs. TAIR 10
Match: AT1G25510.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 134.4 bits (337), Expect = 8.5e-32
Identity = 71/167 (42.51%), Postives = 99/167 (59.28%), Query Frame = 0


Query: 5   PLIKNPSQPSFYYLSLQGISVGGTQLSIPKSTFELHDDGSGGVIIDSGTTITYIESTSFS 64
           PL++N    +FYYL L GISVGG  L IP+S+FE+ + GSGG+IIDSGT +T +++  ++
Sbjct: 318 PLLRNHQLDTFYYLGLTGISVGGELLQIPQSSFEMDESGSGGIIIDSGTAVTRLQTEIYN 377

Query: 65  SLKNELIAQMNLPVDDSGTGGLDLCFNLPAGTTQVEVPKLTFHFKGAD-LELPGENYMIG 124
           SL++  +         +G    D C+NL A TT VEVP + FHF G   L LP +NYMI 
Sbjct: 378 SLRDSFVKGTLDLEKAAGVAMFDTCYNLSAKTT-VEVPTVAFHFPGGKMLALPAKNYMIP 437

Query: 125 DSKTGLLCLAIG-SSRGMSIFGNLQQQNFMVVHDLQEETLSFLPTQC 170
               G  CLA   ++  ++I GN+QQQ   V  DL    + F   +C
Sbjct: 438 VDSVGTFCLAFAPTASSLAIIGNVQQQGTRVTFDLANSLIGFSSNKC 483

[Close]

BLAST of Cmc12g0321571 vs. TAIR 10
Match: AT1G01300.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 126.7 bits (317), Expect = 1.8e-29
Identity = 67/168 (39.88%), Postives = 97/168 (57.74%), Query Frame = 0


Query: 4   TPLIKNPSQPSFYYLSLQGISVGGTQL-SIPKSTFELHDDGSGGVIIDSGTTITYIESTS 63
           TPL+ NP   +FYY+ L GISVGGT++  +  S F+L   G+GGVIIDSGT++T +   +
Sbjct: 318 TPLLSNPKLDTFYYVGLLGISVGGTRVPGVTASLFKLDQIGNGGVIIDSGTSVTRLIRPA 377

Query: 64  FSSLKNELIAQMNLPVDDSGTGGLDLCFNLPAGTTQVEVPKLTFHFKGADLELPGENYMI 123
           + ++++                  D CF+L +   +V+VP +  HF+GAD+ LP  NY+I
Sbjct: 378 YIAMRDAFRVGAKTLKRAPDFSLFDTCFDL-SNMNEVKVPTVVLHFRGADVSLPATNYLI 437

Query: 124 GDSKTGLLCLAI-GSSRGMSIFGNLQQQNFMVVHDLQEETLSFLPTQC 170
                G  C A  G+  G+SI GN+QQQ F VV+DL    + F P  C
Sbjct: 438 PVDTNGKFCFAFAGTMGGLSIIGNIQQQGFRVVYDLASSRVGFAPGGC 484

[Close]

BLAST of Cmc12g0321571 vs. TAIR 10
Match: AT3G61820.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 124.8 bits (312), Expect = 6.8e-29
Identity = 67/168 (39.88%), Postives = 97/168 (57.74%), Query Frame = 0


Query: 4   TPLIKNPSQPSFYYLSLQGISVGGTQL-SIPKSTFELHDDGSGGVIIDSGTTITYIESTS 63
           TPL+ NP   +FYYL L GISVGG+++  + +S F+L   G+GGVIIDSGT++T +   +
Sbjct: 317 TPLLTNPKLDTFYYLQLLGISVGGSRVPGVSESQFKLDATGNGGVIIDSGTSVTRLTQPA 376

Query: 64  FSSLKNELIAQMNLPVDDSGTGGLDLCFNLPAGTTQVEVPKLTFHFKGADLELPGENYMI 123
           + +L++                  D CF+L +G T V+VP + FHF G ++ LP  NY+I
Sbjct: 377 YVALRDAFRLGATKLKRAPSYSLFDTCFDL-SGMTTVKVPTVVFHFGGGEVSLPASNYLI 436

Query: 124 GDSKTGLLCLAIGSSRG-MSIFGNLQQQNFMVVHDLQEETLSFLPTQC 170
             +  G  C A   + G +SI GN+QQQ F V +DL    + FL   C
Sbjct: 437 PVNTEGRFCFAFAGTMGSLSIIGNIQQQGFRVAYDLVGSRVGFLSRAC 483

[Close]

BLAST of Cmc12g0321571 vs. TAIR 10
Match: AT5G33340.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 118.6 bits (296), Expect = 4.9e-27
Identity = 64/167 (38.32%), Postives = 96/167 (57.49%), Query Frame = 0


Query: 3   ATPLIKNPSQPSFYYLSLQGISVGGTQLSIPKSTFELHDDGSGGVIIDSGTTITYIESTS 62
           +TPLI   SQ +FYYL+L+ ISVG  Q+    S  E      G +IIDSGTT+T + +  
Sbjct: 275 STPLIAKASQETFYYLTLKSISVGSKQIQYSGSDSE---SSEGNIIIDSGTTLTLLPTEF 334

Query: 63  FSSLKNELIAQMNLPVDDSGTGGLDLCFNLPAGTTQVEVPKLTFHFKGADLELPGENYMI 122
           +S L++ + + ++         GL LC+   + T  ++VP +T HF GAD++L   N  +
Sbjct: 335 YSELEDAVASSIDAEKKQDPQSGLSLCY---SATGDLKVPVITMHFDGADVKLDSSNAFV 394

Query: 123 GDSKTGLLCLAIGSSRGMSIFGNLQQQNFMVVHDLQEETLSFLPTQC 170
             S+  L+C A   S   SI+GN+ Q NF+V +D   +T+SF PT C
Sbjct: 395 QVSE-DLVCFAFRGSPSFSIYGNVAQMNFLVGYDTVSKTVSFKPTDC 434

The following BLAST results are available for this feature:

BLAST of Cmc12g0321571 vs. NCBI nr


Match Name	E-value	Identity	Description
TYK02448.1	9.0e-92	98.84	aspartic proteinase nepenthesin-1 [Cucumis melo var. makuwa]	[more]
XP_008442220.1	9.0e-92	98.84	PREDICTED: aspartic proteinase nepenthesin-1 [Cucumis melo] >KAA0041170.1 aspart...	[more]
XP_011653928.1	7.1e-89	94.77	aspartic proteinase nepenthesin-1 [Cucumis sativus] >KGN54860.1 hypothetical pro...	[more]
XP_038883313.1	5.7e-86	91.86	aspartic proteinase nepenthesin-1 [Benincasa hispida]	[more]
XP_022928703.1	8.7e-79	84.88	aspartic proteinase nepenthesin-1 [Cucurbita moschata]	[more]

BLAST of Cmc12g0321571 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q766C3	4.7e-43	50.00	Aspartic proteinase nepenthesin-1 OS=Nepenthes gracilis OX=150966 GN=nep1 PE=1 S...	[more]
Q766C2	2.9e-40	46.75	Aspartic proteinase nepenthesin-2 OS=Nepenthes gracilis OX=150966 GN=nep2 PE=1 S...	[more]
Q9LNJ3	2.5e-28	39.88	Aspartyl protease family protein 2 OS=Arabidopsis thaliana OX=3702 GN=APF2 PE=2 ...	[more]
Q6XBF8	6.8e-26	38.32	Aspartic proteinase CDR1 OS=Arabidopsis thaliana OX=3702 GN=CDR1 PE=1 SV=1	[more]
Q9LS40	1.5e-25	35.12	Protein ASPARTIC PROTEASE IN GUARD CELL 1 OS=Arabidopsis thaliana OX=3702 GN=ASP...	[more]

BLAST of Cmc12g0321571 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7TD10	4.4e-92	98.84	Aspartic proteinase nepenthesin-1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C2...	[more]
A0A5D3BTY9	4.4e-92	98.84	Aspartic proteinase nepenthesin-1 OS=Cucumis melo var. makuwa OX=1194695 GN=E567...	[more]
A0A1S3B573	4.4e-92	98.84	aspartic proteinase nepenthesin-1 OS=Cucumis melo OX=3656 GN=LOC103486136 PE=3 S...	[more]
A0A0A0KYT9	3.5e-89	94.77	Peptidase A1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G55468...	[more]
A0A6J1EPU6	4.2e-79	84.88	aspartic proteinase nepenthesin-1 OS=Cucurbita moschata OX=3662 GN=LOC111435534 ...	[more]

BLAST of Cmc12g0321571 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G03200.1	6.3e-67	66.67	Eukaryotic aspartyl protease family protein	[more]
AT1G25510.1	8.5e-32	42.51	Eukaryotic aspartyl protease family protein	[more]
AT1G01300.1	1.8e-29	39.88	Eukaryotic aspartyl protease family protein	[more]
AT3G61820.1	6.8e-29	39.88	Eukaryotic aspartyl protease family protein	[more]
AT5G33340.1	4.9e-27	38.32	Eukaryotic aspartyl protease family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Melon (Charmono) v1.1
Date Performed: 2022-10-13


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR032799	Xylanase inhibitor, C-terminal	PFAM	PF14541	TAXi_C	coord: 15..164 e-value: 4.4E-36 score: 124.1
IPR021109	Aspartic peptidase domain superfamily	GENE3D	2.40.70.10	Acid Proteases	coord: 1..172 e-value: 1.5E-54 score: 186.5
IPR021109	Aspartic peptidase domain superfamily	SUPERFAMILY	50630	Acid proteases	coord: 3..170
None	No IPR available	PANTHER	PTHR47967:SF23	OS08G0469000 PROTEIN	coord: 2..171
None	No IPR available	PANTHER	PTHR47967	OS07G0603500 PROTEIN-RELATED	coord: 2..171
IPR001969	Aspartic peptidase, active site	PROSITE	PS00141	ASP_PROTEASE	coord: 47..58
IPR033121	Peptidase family A1 domain	PROSITE	PS51767	PEPTIDASE_A1	coord: 1..165 score: 23.937359
IPR034161	Pepsin-like domain, plant	CDD	cd05476	pepsin_A_like_plant	coord: 1..169 e-value: 5.95648E-49 score: 157.04

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cmc12g0321571.1	Cmc12g0321571.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006508	proteolysis
molecular_function	GO:0004190	aspartic-type endopeptidase activity