Cmc12g0320951 (gene) Melon (Charmono) v1.1

Overview


Name	Cmc12g0320951
Type	gene
Organism	Cucumis melo L. var. cantalupensis cv. Charmono (Melon (Charmono) v1.1)
Description	Reverse transcriptase
Location	CMiso1.1chr12: 7731606 .. 7732457 (-)
RNA-Seq Expression	Cmc12g0320951
Synteny	Cmc12g0320951

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAAGCAAGAGATAGCTGAATATGTTGATAGATGTTTGATTTGTCAACAAGTTAAACCAGTAAGACAAAGGCCAGGAGGATTTCTTAATCCTTTGCCAGTGCTAGAGTGGAAATGGGAGCATATTACTATGGATTTTCTATTTGGATTACCTCGTACATCCAGTGGACATGATGGTATATGGGTAATAGTAGACAGACTCACCAAGACGACACGATTTATACCGATTAAAATGACATCTACGTTAGACCAGTTAGCGAGATTATATGTTGATAAGATTGTGAGTCAGTATGGAGTACCAGTGTCCATAGTTTCAGAAAGGGATCCGAGGTTTACTTCTAAATTTTGGCCTAGTTTACAGAAAGCAATGGGAACAGGGCTAAAGTTTAGTACATCATTTCATCCCCAAACAGATGGTCAGTCCGAGAGGACCATCCAAACTTTAGAGGACATGTTGAGAGCATGTGTCCTACAACTTAAAGGAAGTTGGGATACCCACTTGCCACTTATGGAGTTTGCTTATAATAATAACTATCAGTCTAGTATCCGTGTGGCACCATATGAAGCCTTATACGGGAGACCATGCAGAACTCCTGTGTGCTGGAATGAAGTGGGAGAGCGGAAGTTAGTAGGTCCTGAGTTGGTTCAGATTACGACAAACAATATTAAGTTGATTAGAGAAAATCTGAGGATAGCCCAAGATCGACAGAAAAGTTATGCGGATAAGCGACGAAGAAACCTAGAATTCCAAGTTGGAGATCAAGTTTTCTTGAAATTATCTCCATGGCGAGGTGTTATTCGTTTCGGAAGAAAAGGTAAGTTAAGTCCTAGATATATTGGGCATATCAGATAA

mRNA sequence

ATGAAGCAAGAGATAGCTGAATATGTTGATAGATGTTTGATTTGTCAACAAGTTAAACCAGTAAGACAAAGGCCAGGAGGATTTCTTAATCCTTTGCCAGTGCTAGAGTGGAAATGGGAGCATATTACTATGGATTTTCTATTTGGATTACCTCGTACATCCAGTGGACATGATGGTATATGGGTAATAGTAGACAGACTCACCAAGACGACACGATTTATACCGATTAAAATGACATCTACGTTAGACCAGTTAGCGAGATTATATGTTGATAAGATTGTGAGTCAGTATGGAGTACCAGTGTCCATAGTTTCAGAAAGGGATCCGAGGTTTACTTCTAAATTTTGGCCTAGTTTACAGAAAGCAATGGGAACAGGGCTAAAGTTTAGTACATCATTTCATCCCCAAACAGATGGTCAGTCCGAGAGGACCATCCAAACTTTAGAGGACATGTTGAGAGCATGTGTCCTACAACTTAAAGGAAGTTGGGATACCCACTTGCCACTTATGGAGTTTGCTTATAATAATAACTATCAGTCTAGTATCCGTGTGGCACCATATGAAGCCTTATACGGGAGACCATGCAGAACTCCTGTGTGCTGGAATGAAGTGGGAGAGCGGAAGTTAGTAGGTCCTGAGTTGGTTCAGATTACGACAAACAATATTAAGTTGATTAGAGAAAATCTGAGGATAGCCCAAGATCGACAGAAAAGTTATGCGGATAAGCGACGAAGAAACCTAGAATTCCAAGTTGGAGATCAAGTTTTCTTGAAATTATCTCCATGGCGAGGTGTTATTCGTTTCGGAAGAAAAGGTAAGTTAAGTCCTAGATATATTGGGCATATCAGATAA

Coding sequence (CDS)

ATGAAGCAAGAGATAGCTGAATATGTTGATAGATGTTTGATTTGTCAACAAGTTAAACCAGTAAGACAAAGGCCAGGAGGATTTCTTAATCCTTTGCCAGTGCTAGAGTGGAAATGGGAGCATATTACTATGGATTTTCTATTTGGATTACCTCGTACATCCAGTGGACATGATGGTATATGGGTAATAGTAGACAGACTCACCAAGACGACACGATTTATACCGATTAAAATGACATCTACGTTAGACCAGTTAGCGAGATTATATGTTGATAAGATTGTGAGTCAGTATGGAGTACCAGTGTCCATAGTTTCAGAAAGGGATCCGAGGTTTACTTCTAAATTTTGGCCTAGTTTACAGAAAGCAATGGGAACAGGGCTAAAGTTTAGTACATCATTTCATCCCCAAACAGATGGTCAGTCCGAGAGGACCATCCAAACTTTAGAGGACATGTTGAGAGCATGTGTCCTACAACTTAAAGGAAGTTGGGATACCCACTTGCCACTTATGGAGTTTGCTTATAATAATAACTATCAGTCTAGTATCCGTGTGGCACCATATGAAGCCTTATACGGGAGACCATGCAGAACTCCTGTGTGCTGGAATGAAGTGGGAGAGCGGAAGTTAGTAGGTCCTGAGTTGGTTCAGATTACGACAAACAATATTAAGTTGATTAGAGAAAATCTGAGGATAGCCCAAGATCGACAGAAAAGTTATGCGGATAAGCGACGAAGAAACCTAGAATTCCAAGTTGGAGATCAAGTTTTCTTGAAATTATCTCCATGGCGAGGTGTTATTCGTTTCGGAAGAAAAGGTAAGTTAAGTCCTAGATATATTGGGCATATCAGATAA

Protein sequence

MKQEIAEYVDRCLICQQVKPVRQRPGGFLNPLPVLEWKWEHITMDFLFGLPRTSSGHDGIWVIVDRLTKTTRFIPIKMTSTLDQLARLYVDKIVSQYGVPVSIVSERDPRFTSKFWPSLQKAMGTGLKFSTSFHPQTDGQSERTIQTLEDMLRACVLQLKGSWDTHLPLMEFAYNNNYQSSIRVAPYEALYGRPCRTPVCWNEVGERKLVGPELVQITTNNIKLIRENLRIAQDRQKSYADKRRRNLEFQVGDQVFLKLSPWRGVIRFGRKGKLSPRYIGHIR

Homology

[Close]

BLAST of Cmc12g0320951 vs. NCBI nr
Match: TYK05214.1 (reverse transcriptase [Cucumis melo var. makuwa])

HSP 1 Score: 572.8 bits (1475), Expect = 1.7e-159
Identity = 275/280 (98.21%), Postives = 277/280 (98.93%), Query Frame = 0


Query: 1   MKQEIAEYVDRCLICQQVKPVRQRPGGFLNPLPVLEWKWEHITMDFLFGLPRTSSGHDGI 60
           MKQEIAEYVDRCLICQQVKPVRQRPGGFLNPLPV EWKWEHITMDFLFGLPRTSSGHDGI
Sbjct: 625 MKQEIAEYVDRCLICQQVKPVRQRPGGFLNPLPVPEWKWEHITMDFLFGLPRTSSGHDGI 684

Query: 61  WVIVDRLTKTTRFIPIKMTSTLDQLARLYVDKIVSQYGVPVSIVSERDPRFTSKFWPSLQ 120
           WVIVDRLTKTTRFIPIKMTSTLDQLARLYVDKIVSQYGVPVSIVS+RDPRFTSKFWPSLQ
Sbjct: 685 WVIVDRLTKTTRFIPIKMTSTLDQLARLYVDKIVSQYGVPVSIVSDRDPRFTSKFWPSLQ 744

Query: 121 KAMGTGLKFSTSFHPQTDGQSERTIQTLEDMLRACVLQLKGSWDTHLPLMEFAYNNNYQS 180
           KAMGTGLKFSTSFHPQTDGQSERTIQTLEDMLRACVLQLKGSWDTHLPLMEFAYNNNYQS
Sbjct: 745 KAMGTGLKFSTSFHPQTDGQSERTIQTLEDMLRACVLQLKGSWDTHLPLMEFAYNNNYQS 804

Query: 181 SIRVAPYEALYGRPCRTPVCWNEVGERKLVGPELVQITTNNIKLIRENLRIAQDRQKSYA 240
           SI +APYEALYGRPCRTPVCWNEVGERKLVGPELVQITTNNIKLIRENLR AQDRQKSYA
Sbjct: 805 SIGMAPYEALYGRPCRTPVCWNEVGERKLVGPELVQITTNNIKLIRENLRKAQDRQKSYA 864

Query: 241 DKRRRNLEFQVGDQVFLKLSPWRGVIRFGRKGKLSPRYIG 281
           DKRRRNLEFQVGDQVFLKLSPWRGVIRFGRKGKLSPRYIG
Sbjct: 865 DKRRRNLEFQVGDQVFLKLSPWRGVIRFGRKGKLSPRYIG 904

[Close]

BLAST of Cmc12g0320951 vs. NCBI nr
Match: KAA0053246.1 (DNA/RNA polymerases superfamily protein [Cucumis melo var. makuwa])

HSP 1 Score: 572.8 bits (1475), Expect = 1.7e-159
Identity = 275/280 (98.21%), Postives = 277/280 (98.93%), Query Frame = 0


Query: 1   MKQEIAEYVDRCLICQQVKPVRQRPGGFLNPLPVLEWKWEHITMDFLFGLPRTSSGHDGI 60
           MKQEIAEYVDRCLICQQVKPVRQRPGGFLNPLPV EWKWEHITMDFLFGLPRTSSGHDGI
Sbjct: 230 MKQEIAEYVDRCLICQQVKPVRQRPGGFLNPLPVPEWKWEHITMDFLFGLPRTSSGHDGI 289

Query: 61  WVIVDRLTKTTRFIPIKMTSTLDQLARLYVDKIVSQYGVPVSIVSERDPRFTSKFWPSLQ 120
           WVIVDRLTKTTRFIPIKMTSTLDQLARLYVDKIVSQYGVPVSIVS+RDPRFTSKFWPSLQ
Sbjct: 290 WVIVDRLTKTTRFIPIKMTSTLDQLARLYVDKIVSQYGVPVSIVSDRDPRFTSKFWPSLQ 349

Query: 121 KAMGTGLKFSTSFHPQTDGQSERTIQTLEDMLRACVLQLKGSWDTHLPLMEFAYNNNYQS 180
           KAMGTGLKFSTSFHPQTDGQSERTIQTLEDMLRACVLQLKGSWDTHLPLMEFAYNNNYQS
Sbjct: 350 KAMGTGLKFSTSFHPQTDGQSERTIQTLEDMLRACVLQLKGSWDTHLPLMEFAYNNNYQS 409

Query: 181 SIRVAPYEALYGRPCRTPVCWNEVGERKLVGPELVQITTNNIKLIRENLRIAQDRQKSYA 240
           SI +APYEALYGRPCRTPVCWNEVGERKLVGPELVQITTNNIKLIRENLR AQDRQKSYA
Sbjct: 410 SIGMAPYEALYGRPCRTPVCWNEVGERKLVGPELVQITTNNIKLIRENLRKAQDRQKSYA 469

Query: 241 DKRRRNLEFQVGDQVFLKLSPWRGVIRFGRKGKLSPRYIG 281
           DKRRRNLEFQVGDQVFLKLSPWRGVIRFGRKGKLSPRYIG
Sbjct: 470 DKRRRNLEFQVGDQVFLKLSPWRGVIRFGRKGKLSPRYIG 509

[Close]

BLAST of Cmc12g0320951 vs. NCBI nr
Match: KAA0050493.1 (DNA/RNA polymerases superfamily protein [Cucumis melo var. makuwa])

HSP 1 Score: 572.8 bits (1475), Expect = 1.7e-159
Identity = 275/280 (98.21%), Postives = 277/280 (98.93%), Query Frame = 0


Query: 1    MKQEIAEYVDRCLICQQVKPVRQRPGGFLNPLPVLEWKWEHITMDFLFGLPRTSSGHDGI 60
            MKQEIAEYVDRCLICQQVKPVRQRPGGFLNPLPV EWKWEHITMDFLFGLPRTSSGHDGI
Sbjct: 752  MKQEIAEYVDRCLICQQVKPVRQRPGGFLNPLPVPEWKWEHITMDFLFGLPRTSSGHDGI 811

Query: 61   WVIVDRLTKTTRFIPIKMTSTLDQLARLYVDKIVSQYGVPVSIVSERDPRFTSKFWPSLQ 120
            WVIVDRLTKTTRFIPIKMTSTLDQLARLYVDKIVSQYGVPVSIVS+RDPRFTSKFWPSLQ
Sbjct: 812  WVIVDRLTKTTRFIPIKMTSTLDQLARLYVDKIVSQYGVPVSIVSDRDPRFTSKFWPSLQ 871

Query: 121  KAMGTGLKFSTSFHPQTDGQSERTIQTLEDMLRACVLQLKGSWDTHLPLMEFAYNNNYQS 180
            KAMGTGLKFSTSFHPQTDGQSERTIQTLEDMLRACVLQLKGSWDTHLPLMEFAYNNNYQS
Sbjct: 872  KAMGTGLKFSTSFHPQTDGQSERTIQTLEDMLRACVLQLKGSWDTHLPLMEFAYNNNYQS 931

Query: 181  SIRVAPYEALYGRPCRTPVCWNEVGERKLVGPELVQITTNNIKLIRENLRIAQDRQKSYA 240
            SI +APYEALYGRPCRTPVCWNEVGERKLVGPELVQITTNNIKLIRENLR AQDRQKSYA
Sbjct: 932  SIGMAPYEALYGRPCRTPVCWNEVGERKLVGPELVQITTNNIKLIRENLRKAQDRQKSYA 991

Query: 241  DKRRRNLEFQVGDQVFLKLSPWRGVIRFGRKGKLSPRYIG 281
            DKRRRNLEFQVGDQVFLKLSPWRGVIRFGRKGKLSPRYIG
Sbjct: 992  DKRRRNLEFQVGDQVFLKLSPWRGVIRFGRKGKLSPRYIG 1031

[Close]

BLAST of Cmc12g0320951 vs. NCBI nr
Match: KAA0059912.1 (reverse transcriptase [Cucumis melo var. makuwa])

HSP 1 Score: 572.8 bits (1475), Expect = 1.7e-159
Identity = 275/280 (98.21%), Postives = 277/280 (98.93%), Query Frame = 0


Query: 1   MKQEIAEYVDRCLICQQVKPVRQRPGGFLNPLPVLEWKWEHITMDFLFGLPRTSSGHDGI 60
           MKQEIAEYVDRCLICQQVKPVRQRPGGFLNPLPV EWKWEHITMDFLFGLPRTSSGHDGI
Sbjct: 420 MKQEIAEYVDRCLICQQVKPVRQRPGGFLNPLPVPEWKWEHITMDFLFGLPRTSSGHDGI 479

Query: 61  WVIVDRLTKTTRFIPIKMTSTLDQLARLYVDKIVSQYGVPVSIVSERDPRFTSKFWPSLQ 120
           WVIVDRLTKTTRFIPIKMTSTLDQLARLYVDKIVSQYGVPVSIVS+RDPRFTSKFWPSLQ
Sbjct: 480 WVIVDRLTKTTRFIPIKMTSTLDQLARLYVDKIVSQYGVPVSIVSDRDPRFTSKFWPSLQ 539

Query: 121 KAMGTGLKFSTSFHPQTDGQSERTIQTLEDMLRACVLQLKGSWDTHLPLMEFAYNNNYQS 180
           KAMGTGLKFSTSFHPQTDGQSERTIQTLEDMLRACVLQLKGSWDTHLPLMEFAYNNNYQS
Sbjct: 540 KAMGTGLKFSTSFHPQTDGQSERTIQTLEDMLRACVLQLKGSWDTHLPLMEFAYNNNYQS 599

Query: 181 SIRVAPYEALYGRPCRTPVCWNEVGERKLVGPELVQITTNNIKLIRENLRIAQDRQKSYA 240
           SI +APYEALYGRPCRTPVCWNEVGERKLVGPELVQITTNNIKLIRENLR AQDRQKSYA
Sbjct: 600 SIGMAPYEALYGRPCRTPVCWNEVGERKLVGPELVQITTNNIKLIRENLRKAQDRQKSYA 659

Query: 241 DKRRRNLEFQVGDQVFLKLSPWRGVIRFGRKGKLSPRYIG 281
           DKRRRNLEFQVGDQVFLKLSPWRGVIRFGRKGKLSPRYIG
Sbjct: 660 DKRRRNLEFQVGDQVFLKLSPWRGVIRFGRKGKLSPRYIG 699

[Close]

BLAST of Cmc12g0320951 vs. NCBI nr
Match: KAA0050527.1 (DNA/RNA polymerases superfamily protein [Cucumis melo var. makuwa])

HSP 1 Score: 572.8 bits (1475), Expect = 1.7e-159
Identity = 275/280 (98.21%), Postives = 277/280 (98.93%), Query Frame = 0


Query: 1    MKQEIAEYVDRCLICQQVKPVRQRPGGFLNPLPVLEWKWEHITMDFLFGLPRTSSGHDGI 60
            MKQEIAEYVDRCLICQQVKPVRQRPGGFLNPLPV EWKWEHITMDFLFGLPRTSSGHDGI
Sbjct: 859  MKQEIAEYVDRCLICQQVKPVRQRPGGFLNPLPVPEWKWEHITMDFLFGLPRTSSGHDGI 918

Query: 61   WVIVDRLTKTTRFIPIKMTSTLDQLARLYVDKIVSQYGVPVSIVSERDPRFTSKFWPSLQ 120
            WVIVDRLTKTTRFIPIKMTSTLDQLARLYVDKIVSQYGVPVSIVS+RDPRFTSKFWPSLQ
Sbjct: 919  WVIVDRLTKTTRFIPIKMTSTLDQLARLYVDKIVSQYGVPVSIVSDRDPRFTSKFWPSLQ 978

Query: 121  KAMGTGLKFSTSFHPQTDGQSERTIQTLEDMLRACVLQLKGSWDTHLPLMEFAYNNNYQS 180
            KAMGTGLKFSTSFHPQTDGQSERTIQTLEDMLRACVLQLKGSWDTHLPLMEFAYNNNYQS
Sbjct: 979  KAMGTGLKFSTSFHPQTDGQSERTIQTLEDMLRACVLQLKGSWDTHLPLMEFAYNNNYQS 1038

Query: 181  SIRVAPYEALYGRPCRTPVCWNEVGERKLVGPELVQITTNNIKLIRENLRIAQDRQKSYA 240
            SI +APYEALYGRPCRTPVCWNEVGERKLVGPELVQITTNNIKLIRENLR AQDRQKSYA
Sbjct: 1039 SIGMAPYEALYGRPCRTPVCWNEVGERKLVGPELVQITTNNIKLIRENLRKAQDRQKSYA 1098

Query: 241  DKRRRNLEFQVGDQVFLKLSPWRGVIRFGRKGKLSPRYIG 281
            DKRRRNLEFQVGDQVFLKLSPWRGVIRFGRKGKLSPRYIG
Sbjct: 1099 DKRRRNLEFQVGDQVFLKLSPWRGVIRFGRKGKLSPRYIG 1138

[Close]

BLAST of Cmc12g0320951 vs. ExPASy Swiss-Prot
Match: Q7LHG5 (Transposon Ty3-I Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY3B-I PE=1 SV=2)

HSP 1 Score: 166.4 bits (420), Expect = 4.7e-40
Identity = 101/285 (35.44%), Postives = 147/285 (51.58%), Query Frame = 0


Query: 1    MKQEIAEYVDRCLICQQVKPVRQRPGGFLNPLPVLEWKWEHITMDFLFGLPRTSSGHDGI 60
            ++  I +Y+  C+ CQ +K  R R  G L PLP+ E +W  I+MDF+ GLP TS+  + I
Sbjct: 1157 LQHSIIQYIRTCVQCQLIKSHRPRLHGLLQPLPIAEGRWLDISMDFVTGLPPTSNNLNMI 1216

Query: 61   WVIVDRLTKTTRFIPIKMTSTLDQLARLYVDKIVSQYGVPVSIVSERDPRFTSKFWPSLQ 120
             V+VDR +K   FI  + T    QL  L    I S +G P +I S+RD R T+  +  L 
Sbjct: 1217 LVVVDRFSKRAHFIATRKTLDATQLIDLLFRYIFSYHGFPRTITSDRDVRMTADKYQELT 1276

Query: 121  KAMGTGLKFSTSFHPQTDGQSERTIQTLEDMLRACVLQLKGSWDTHLPLMEFAYNNNYQS 180
            K +G     S++ HPQTDGQSERTIQTL  +LRA V     +W  +LP +EF YN+    
Sbjct: 1277 KRLGIKSTMSSANHPQTDGQSERTIQTLNRLLRAYVSTNIQNWHVYLPQIEFVYNSTPTR 1336

Query: 181  SIRVAPYEALYGRPCRTPVCW--NEVGERKLVGPELVQITTNNIKLIRENLRIAQDRQKS 240
            ++  +P+E   G    TP     +EV  R     EL +         +E L  AQ   ++
Sbjct: 1337 TLGKSPFEIDLGYLPNTPAIKSDDEVNARSFTAVELAKHLKALTIQTKEQLEHAQIEMET 1396

Query: 241  YADKRRRNLEFQVGDQVFLKLSPWRGVIRFGRKGKLSPRYIGHIR 284
              ++RR+ L   +GD V +    +    + G   K+   Y+G  R
Sbjct: 1397 NNNQRRKPLLLNIGDHVLVHRDAY---FKKGAYMKVQQIYVGPFR 1438

[Close]

BLAST of Cmc12g0320951 vs. ExPASy Swiss-Prot
Match: Q99315 (Transposon Ty3-G Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY3B-G PE=1 SV=3)

HSP 1 Score: 164.9 bits (416), Expect = 1.4e-39
Identity = 100/285 (35.09%), Postives = 146/285 (51.23%), Query Frame = 0


Query: 1    MKQEIAEYVDRCLICQQVKPVRQRPGGFLNPLPVLEWKWEHITMDFLFGLPRTSSGHDGI 60
            ++  I +Y+  C+ CQ +K  R R  G L PLP+ E +W  I+MDF+ GLP TS+  + I
Sbjct: 1131 LQHSIIQYIRTCVQCQLIKSHRPRLHGLLQPLPIAEGRWLDISMDFVTGLPPTSNNLNMI 1190

Query: 61   WVIVDRLTKTTRFIPIKMTSTLDQLARLYVDKIVSQYGVPVSIVSERDPRFTSKFWPSLQ 120
             V+VDR +K   FI  + T    QL  L    I S +G P +I S+RD R T+  +  L 
Sbjct: 1191 LVVVDRFSKRAHFIATRKTLDATQLIDLLFRYIFSYHGFPRTITSDRDVRMTADKYQELT 1250

Query: 121  KAMGTGLKFSTSFHPQTDGQSERTIQTLEDMLRACVLQLKGSWDTHLPLMEFAYNNNYQS 180
            K +G     S++ HPQTDGQSERTIQTL  +LRA       +W  +LP +EF YN+    
Sbjct: 1251 KRLGIKSTMSSANHPQTDGQSERTIQTLNRLLRAYASTNIQNWHVYLPQIEFVYNSTPTR 1310

Query: 181  SIRVAPYEALYGRPCRTPVCW--NEVGERKLVGPELVQITTNNIKLIRENLRIAQDRQKS 240
            ++  +P+E   G    TP     +EV  R     EL +         +E L  AQ   ++
Sbjct: 1311 TLGKSPFEIDLGYLPNTPAIKSDDEVNARSFTAVELAKHLKALTIQTKEQLEHAQIEMET 1370

Query: 241  YADKRRRNLEFQVGDQVFLKLSPWRGVIRFGRKGKLSPRYIGHIR 284
              ++RR+ L   +GD V +    +    + G   K+   Y+G  R
Sbjct: 1371 NNNQRRKPLLLNIGDHVLVHRDAY---FKKGAYMKVQQIYVGPFR 1412

[Close]

BLAST of Cmc12g0320951 vs. ExPASy Swiss-Prot
Match: Q9UR07 (Transposon Tf2-11 polyprotein OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=Tf2-11 PE=3 SV=1)

HSP 1 Score: 161.0 bits (406), Expect = 2.0e-38
Identity = 90/282 (31.91%), Postives = 153/282 (54.26%), Query Frame = 0


Query: 1    MKQEIAEYVDRCLICQQVKPVRQRPGGFLNPLPVLEWKWEHITMDFLFGLPRTSSGHDGI 60
            ++++I EYV  C  CQ  K    +P G L P+P  E  WE ++MDF+  LP  SSG++ +
Sbjct: 946  IRKQIQEYVQNCHTCQINKSRNHKPYGPLQPIPPSERPWESLSMDFITALPE-SSGYNAL 1005

Query: 61   WVIVDRLTKTTRFIPIKMTSTLDQLARLYVDKIVSQYGVPVSIVSERDPRFTSKFWPSLQ 120
            +V+VDR +K    +P   + T +Q AR++  ++++ +G P  I+++ D  FTS+ W    
Sbjct: 1006 FVVVDRFSKMAILVPCTKSITAEQTARMFDQRVIAYFGNPKEIIADNDHIFTSQTWKDFA 1065

Query: 121  KAMGTGLKFSTSFHPQTDGQSERTIQTLEDMLRACVLQLKGSWDTHLPLMEFAYNNNYQS 180
                  +KFS  + PQTDGQ+ERT QT+E +LR        +W  H+ L++ +YNN   S
Sbjct: 1066 HKYNFVMKFSLPYRPQTDGQTERTNQTVEKLLRCVCSTHPNTWVDHISLVQQSYNNAIHS 1125

Query: 181  SIRVAPYEALYG-RPCRTPVCWNEVGERKLVGPELVQITTNNIKLIRENLRIAQDRQKSY 240
            + ++ P+E ++   P  +P+      ++     E  Q T    + ++E+L     + K Y
Sbjct: 1126 ATQMTPFEIVHRYSPALSPLELPSFSDKT---DENSQETIQVFQTVKEHLNTNNIKMKKY 1185

Query: 241  ADKRRRNL-EFQVGDQVFLKLSPWRGVIRFGRKGKLSPRYIG 281
             D + + + EFQ GD V +K +   G +   +  KL+P + G
Sbjct: 1186 FDMKIQEIEEFQPGDLVMVKRTK-TGFLH--KSNKLAPSFAG 1220

[Close]

BLAST of Cmc12g0320951 vs. ExPASy Swiss-Prot
Match: P0CT41 (Transposon Tf2-12 polyprotein OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=Tf2-12 PE=3 SV=1)

HSP 1 Score: 161.0 bits (406), Expect = 2.0e-38
Identity = 90/282 (31.91%), Postives = 153/282 (54.26%), Query Frame = 0


Query: 1    MKQEIAEYVDRCLICQQVKPVRQRPGGFLNPLPVLEWKWEHITMDFLFGLPRTSSGHDGI 60
            ++++I EYV  C  CQ  K    +P G L P+P  E  WE ++MDF+  LP  SSG++ +
Sbjct: 946  IRKQIQEYVQNCHTCQINKSRNHKPYGPLQPIPPSERPWESLSMDFITALPE-SSGYNAL 1005

Query: 61   WVIVDRLTKTTRFIPIKMTSTLDQLARLYVDKIVSQYGVPVSIVSERDPRFTSKFWPSLQ 120
            +V+VDR +K    +P   + T +Q AR++  ++++ +G P  I+++ D  FTS+ W    
Sbjct: 1006 FVVVDRFSKMAILVPCTKSITAEQTARMFDQRVIAYFGNPKEIIADNDHIFTSQTWKDFA 1065

Query: 121  KAMGTGLKFSTSFHPQTDGQSERTIQTLEDMLRACVLQLKGSWDTHLPLMEFAYNNNYQS 180
                  +KFS  + PQTDGQ+ERT QT+E +LR        +W  H+ L++ +YNN   S
Sbjct: 1066 HKYNFVMKFSLPYRPQTDGQTERTNQTVEKLLRCVCSTHPNTWVDHISLVQQSYNNAIHS 1125

Query: 181  SIRVAPYEALYG-RPCRTPVCWNEVGERKLVGPELVQITTNNIKLIRENLRIAQDRQKSY 240
            + ++ P+E ++   P  +P+      ++     E  Q T    + ++E+L     + K Y
Sbjct: 1126 ATQMTPFEIVHRYSPALSPLELPSFSDKT---DENSQETIQVFQTVKEHLNTNNIKMKKY 1185

Query: 241  ADKRRRNL-EFQVGDQVFLKLSPWRGVIRFGRKGKLSPRYIG 281
             D + + + EFQ GD V +K +   G +   +  KL+P + G
Sbjct: 1186 FDMKIQEIEEFQPGDLVMVKRTK-TGFLH--KSNKLAPSFAG 1220

[Close]

BLAST of Cmc12g0320951 vs. ExPASy Swiss-Prot
Match: P0CT34 (Transposon Tf2-1 polyprotein OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=Tf2-1 PE=3 SV=1)

HSP 1 Score: 161.0 bits (406), Expect = 2.0e-38
Identity = 90/282 (31.91%), Postives = 153/282 (54.26%), Query Frame = 0


Query: 1    MKQEIAEYVDRCLICQQVKPVRQRPGGFLNPLPVLEWKWEHITMDFLFGLPRTSSGHDGI 60
            ++++I EYV  C  CQ  K    +P G L P+P  E  WE ++MDF+  LP  SSG++ +
Sbjct: 946  IRKQIQEYVQNCHTCQINKSRNHKPYGPLQPIPPSERPWESLSMDFITALPE-SSGYNAL 1005

Query: 61   WVIVDRLTKTTRFIPIKMTSTLDQLARLYVDKIVSQYGVPVSIVSERDPRFTSKFWPSLQ 120
            +V+VDR +K    +P   + T +Q AR++  ++++ +G P  I+++ D  FTS+ W    
Sbjct: 1006 FVVVDRFSKMAILVPCTKSITAEQTARMFDQRVIAYFGNPKEIIADNDHIFTSQTWKDFA 1065

Query: 121  KAMGTGLKFSTSFHPQTDGQSERTIQTLEDMLRACVLQLKGSWDTHLPLMEFAYNNNYQS 180
                  +KFS  + PQTDGQ+ERT QT+E +LR        +W  H+ L++ +YNN   S
Sbjct: 1066 HKYNFVMKFSLPYRPQTDGQTERTNQTVEKLLRCVCSTHPNTWVDHISLVQQSYNNAIHS 1125

Query: 181  SIRVAPYEALYG-RPCRTPVCWNEVGERKLVGPELVQITTNNIKLIRENLRIAQDRQKSY 240
            + ++ P+E ++   P  +P+      ++     E  Q T    + ++E+L     + K Y
Sbjct: 1126 ATQMTPFEIVHRYSPALSPLELPSFSDKT---DENSQETIQVFQTVKEHLNTNNIKMKKY 1185

Query: 241  ADKRRRNL-EFQVGDQVFLKLSPWRGVIRFGRKGKLSPRYIG 281
             D + + + EFQ GD V +K +   G +   +  KL+P + G
Sbjct: 1186 FDMKIQEIEEFQPGDLVMVKRTK-TGFLH--KSNKLAPSFAG 1220

[Close]

BLAST of Cmc12g0320951 vs. ExPASy TrEMBL
Match: A0A5A7U2V7 (Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold374G00630 PE=4 SV=1)

HSP 1 Score: 572.8 bits (1475), Expect = 8.0e-160
Identity = 275/280 (98.21%), Postives = 277/280 (98.93%), Query Frame = 0


Query: 1    MKQEIAEYVDRCLICQQVKPVRQRPGGFLNPLPVLEWKWEHITMDFLFGLPRTSSGHDGI 60
            MKQEIAEYVDRCLICQQVKPVRQRPGGFLNPLPV EWKWEHITMDFLFGLPRTSSGHDGI
Sbjct: 1160 MKQEIAEYVDRCLICQQVKPVRQRPGGFLNPLPVPEWKWEHITMDFLFGLPRTSSGHDGI 1219

Query: 61   WVIVDRLTKTTRFIPIKMTSTLDQLARLYVDKIVSQYGVPVSIVSERDPRFTSKFWPSLQ 120
            WVIVDRLTKTTRFIPIKMTSTLDQLARLYVDKIVSQYGVPVSIVS+RDPRFTSKFWPSLQ
Sbjct: 1220 WVIVDRLTKTTRFIPIKMTSTLDQLARLYVDKIVSQYGVPVSIVSDRDPRFTSKFWPSLQ 1279

Query: 121  KAMGTGLKFSTSFHPQTDGQSERTIQTLEDMLRACVLQLKGSWDTHLPLMEFAYNNNYQS 180
            KAMGTGLKFSTSFHPQTDGQSERTIQTLEDMLRACVLQLKGSWDTHLPLMEFAYNNNYQS
Sbjct: 1280 KAMGTGLKFSTSFHPQTDGQSERTIQTLEDMLRACVLQLKGSWDTHLPLMEFAYNNNYQS 1339

Query: 181  SIRVAPYEALYGRPCRTPVCWNEVGERKLVGPELVQITTNNIKLIRENLRIAQDRQKSYA 240
            SI +APYEALYGRPCRTPVCWNEVGERKLVGPELVQITTNNIKLIRENLR AQDRQKSYA
Sbjct: 1340 SIGMAPYEALYGRPCRTPVCWNEVGERKLVGPELVQITTNNIKLIRENLRKAQDRQKSYA 1399

Query: 241  DKRRRNLEFQVGDQVFLKLSPWRGVIRFGRKGKLSPRYIG 281
            DKRRRNLEFQVGDQVFLKLSPWRGVIRFGRKGKLSPRYIG
Sbjct: 1400 DKRRRNLEFQVGDQVFLKLSPWRGVIRFGRKGKLSPRYIG 1439

[Close]

BLAST of Cmc12g0320951 vs. ExPASy TrEMBL
Match: A0A5D3BS67 (Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold509G00050 PE=4 SV=1)

HSP 1 Score: 572.8 bits (1475), Expect = 8.0e-160
Identity = 275/280 (98.21%), Postives = 277/280 (98.93%), Query Frame = 0


Query: 1    MKQEIAEYVDRCLICQQVKPVRQRPGGFLNPLPVLEWKWEHITMDFLFGLPRTSSGHDGI 60
            MKQEIAEYVDRCLICQQVKPVRQRPGGFLNPLPV EWKWEHITMDFLFGLPRTSSGHDGI
Sbjct: 1198 MKQEIAEYVDRCLICQQVKPVRQRPGGFLNPLPVPEWKWEHITMDFLFGLPRTSSGHDGI 1257

Query: 61   WVIVDRLTKTTRFIPIKMTSTLDQLARLYVDKIVSQYGVPVSIVSERDPRFTSKFWPSLQ 120
            WVIVDRLTKTTRFIPIKMTSTLDQLARLYVDKIVSQYGVPVSIVS+RDPRFTSKFWPSLQ
Sbjct: 1258 WVIVDRLTKTTRFIPIKMTSTLDQLARLYVDKIVSQYGVPVSIVSDRDPRFTSKFWPSLQ 1317

Query: 121  KAMGTGLKFSTSFHPQTDGQSERTIQTLEDMLRACVLQLKGSWDTHLPLMEFAYNNNYQS 180
            KAMGTGLKFSTSFHPQTDGQSERTIQTLEDMLRACVLQLKGSWDTHLPLMEFAYNNNYQS
Sbjct: 1318 KAMGTGLKFSTSFHPQTDGQSERTIQTLEDMLRACVLQLKGSWDTHLPLMEFAYNNNYQS 1377

Query: 181  SIRVAPYEALYGRPCRTPVCWNEVGERKLVGPELVQITTNNIKLIRENLRIAQDRQKSYA 240
            SI +APYEALYGRPCRTPVCWNEVGERKLVGPELVQITTNNIKLIRENLR AQDRQKSYA
Sbjct: 1378 SIGMAPYEALYGRPCRTPVCWNEVGERKLVGPELVQITTNNIKLIRENLRKAQDRQKSYA 1437

Query: 241  DKRRRNLEFQVGDQVFLKLSPWRGVIRFGRKGKLSPRYIG 281
            DKRRRNLEFQVGDQVFLKLSPWRGVIRFGRKGKLSPRYIG
Sbjct: 1438 DKRRRNLEFQVGDQVFLKLSPWRGVIRFGRKGKLSPRYIG 1477

[Close]

BLAST of Cmc12g0320951 vs. ExPASy TrEMBL
Match: A0A5D3BHI1 (Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold115G00450 PE=4 SV=1)

HSP 1 Score: 572.8 bits (1475), Expect = 8.0e-160
Identity = 275/280 (98.21%), Postives = 277/280 (98.93%), Query Frame = 0


Query: 1    MKQEIAEYVDRCLICQQVKPVRQRPGGFLNPLPVLEWKWEHITMDFLFGLPRTSSGHDGI 60
            MKQEIAEYVDRCLICQQVKPVRQRPGGFLNPLPV EWKWEHITMDFLFGLPRTSSGHDGI
Sbjct: 1198 MKQEIAEYVDRCLICQQVKPVRQRPGGFLNPLPVPEWKWEHITMDFLFGLPRTSSGHDGI 1257

Query: 61   WVIVDRLTKTTRFIPIKMTSTLDQLARLYVDKIVSQYGVPVSIVSERDPRFTSKFWPSLQ 120
            WVIVDRLTKTTRFIPIKMTSTLDQLARLYVDKIVSQYGVPVSIVS+RDPRFTSKFWPSLQ
Sbjct: 1258 WVIVDRLTKTTRFIPIKMTSTLDQLARLYVDKIVSQYGVPVSIVSDRDPRFTSKFWPSLQ 1317

Query: 121  KAMGTGLKFSTSFHPQTDGQSERTIQTLEDMLRACVLQLKGSWDTHLPLMEFAYNNNYQS 180
            KAMGTGLKFSTSFHPQTDGQSERTIQTLEDMLRACVLQLKGSWDTHLPLMEFAYNNNYQS
Sbjct: 1318 KAMGTGLKFSTSFHPQTDGQSERTIQTLEDMLRACVLQLKGSWDTHLPLMEFAYNNNYQS 1377

Query: 181  SIRVAPYEALYGRPCRTPVCWNEVGERKLVGPELVQITTNNIKLIRENLRIAQDRQKSYA 240
            SI +APYEALYGRPCRTPVCWNEVGERKLVGPELVQITTNNIKLIRENLR AQDRQKSYA
Sbjct: 1378 SIGMAPYEALYGRPCRTPVCWNEVGERKLVGPELVQITTNNIKLIRENLRKAQDRQKSYA 1437

Query: 241  DKRRRNLEFQVGDQVFLKLSPWRGVIRFGRKGKLSPRYIG 281
            DKRRRNLEFQVGDQVFLKLSPWRGVIRFGRKGKLSPRYIG
Sbjct: 1438 DKRRRNLEFQVGDQVFLKLSPWRGVIRFGRKGKLSPRYIG 1477

[Close]

BLAST of Cmc12g0320951 vs. ExPASy TrEMBL
Match: A0A5A7T1Y5 (Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold436G00980 PE=4 SV=1)

HSP 1 Score: 572.8 bits (1475), Expect = 8.0e-160
Identity = 275/280 (98.21%), Postives = 277/280 (98.93%), Query Frame = 0


Query: 1    MKQEIAEYVDRCLICQQVKPVRQRPGGFLNPLPVLEWKWEHITMDFLFGLPRTSSGHDGI 60
            MKQEIAEYVDRCLICQQVKPVRQRPGGFLNPLPV EWKWEHITMDFLFGLPRTSSGHDGI
Sbjct: 1198 MKQEIAEYVDRCLICQQVKPVRQRPGGFLNPLPVPEWKWEHITMDFLFGLPRTSSGHDGI 1257

Query: 61   WVIVDRLTKTTRFIPIKMTSTLDQLARLYVDKIVSQYGVPVSIVSERDPRFTSKFWPSLQ 120
            WVIVDRLTKTTRFIPIKMTSTLDQLARLYVDKIVSQYGVPVSIVS+RDPRFTSKFWPSLQ
Sbjct: 1258 WVIVDRLTKTTRFIPIKMTSTLDQLARLYVDKIVSQYGVPVSIVSDRDPRFTSKFWPSLQ 1317

Query: 121  KAMGTGLKFSTSFHPQTDGQSERTIQTLEDMLRACVLQLKGSWDTHLPLMEFAYNNNYQS 180
            KAMGTGLKFSTSFHPQTDGQSERTIQTLEDMLRACVLQLKGSWDTHLPLMEFAYNNNYQS
Sbjct: 1318 KAMGTGLKFSTSFHPQTDGQSERTIQTLEDMLRACVLQLKGSWDTHLPLMEFAYNNNYQS 1377

Query: 181  SIRVAPYEALYGRPCRTPVCWNEVGERKLVGPELVQITTNNIKLIRENLRIAQDRQKSYA 240
            SI +APYEALYGRPCRTPVCWNEVGERKLVGPELVQITTNNIKLIRENLR AQDRQKSYA
Sbjct: 1378 SIGMAPYEALYGRPCRTPVCWNEVGERKLVGPELVQITTNNIKLIRENLRKAQDRQKSYA 1437

Query: 241  DKRRRNLEFQVGDQVFLKLSPWRGVIRFGRKGKLSPRYIG 281
            DKRRRNLEFQVGDQVFLKLSPWRGVIRFGRKGKLSPRYIG
Sbjct: 1438 DKRRRNLEFQVGDQVFLKLSPWRGVIRFGRKGKLSPRYIG 1477

[Close]

BLAST of Cmc12g0320951 vs. ExPASy TrEMBL
Match: A0A5A7UUL6 (Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold132G00090 PE=4 SV=1)

HSP 1 Score: 572.8 bits (1475), Expect = 8.0e-160
Identity = 275/280 (98.21%), Postives = 277/280 (98.93%), Query Frame = 0


Query: 1    MKQEIAEYVDRCLICQQVKPVRQRPGGFLNPLPVLEWKWEHITMDFLFGLPRTSSGHDGI 60
            MKQEIAEYVDRCLICQQVKPVRQRPGGFLNPLPV EWKWEHITMDFLFGLPRTSSGHDGI
Sbjct: 830  MKQEIAEYVDRCLICQQVKPVRQRPGGFLNPLPVPEWKWEHITMDFLFGLPRTSSGHDGI 889

Query: 61   WVIVDRLTKTTRFIPIKMTSTLDQLARLYVDKIVSQYGVPVSIVSERDPRFTSKFWPSLQ 120
            WVIVDRLTKTTRFIPIKMTSTLDQLARLYVDKIVSQYGVPVSIVS+RDPRFTSKFWPSLQ
Sbjct: 890  WVIVDRLTKTTRFIPIKMTSTLDQLARLYVDKIVSQYGVPVSIVSDRDPRFTSKFWPSLQ 949

Query: 121  KAMGTGLKFSTSFHPQTDGQSERTIQTLEDMLRACVLQLKGSWDTHLPLMEFAYNNNYQS 180
            KAMGTGLKFSTSFHPQTDGQSERTIQTLEDMLRACVLQLKGSWDTHLPLMEFAYNNNYQS
Sbjct: 950  KAMGTGLKFSTSFHPQTDGQSERTIQTLEDMLRACVLQLKGSWDTHLPLMEFAYNNNYQS 1009

Query: 181  SIRVAPYEALYGRPCRTPVCWNEVGERKLVGPELVQITTNNIKLIRENLRIAQDRQKSYA 240
            SI +APYEALYGRPCRTPVCWNEVGERKLVGPELVQITTNNIKLIRENLR AQDRQKSYA
Sbjct: 1010 SIGMAPYEALYGRPCRTPVCWNEVGERKLVGPELVQITTNNIKLIRENLRKAQDRQKSYA 1069

Query: 241  DKRRRNLEFQVGDQVFLKLSPWRGVIRFGRKGKLSPRYIG 281
            DKRRRNLEFQVGDQVFLKLSPWRGVIRFGRKGKLSPRYIG
Sbjct: 1070 DKRRRNLEFQVGDQVFLKLSPWRGVIRFGRKGKLSPRYIG 1109

The following BLAST results are available for this feature:

BLAST of Cmc12g0320951 vs. NCBI nr


Match Name	E-value	Identity	Description
TYK05214.1	1.7e-159	98.21	reverse transcriptase [Cucumis melo var. makuwa]	[more]
KAA0053246.1	1.7e-159	98.21	DNA/RNA polymerases superfamily protein [Cucumis melo var. makuwa]	[more]
KAA0050493.1	1.7e-159	98.21	DNA/RNA polymerases superfamily protein [Cucumis melo var. makuwa]	[more]
KAA0059912.1	1.7e-159	98.21	reverse transcriptase [Cucumis melo var. makuwa]	[more]
KAA0050527.1	1.7e-159	98.21	DNA/RNA polymerases superfamily protein [Cucumis melo var. makuwa]	[more]

BLAST of Cmc12g0320951 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q7LHG5	4.7e-40	35.44	Transposon Ty3-I Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 20...	[more]
Q99315	1.4e-39	35.09	Transposon Ty3-G Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 20...	[more]
Q9UR07	2.0e-38	31.91	Transposon Tf2-11 polyprotein OS=Schizosaccharomyces pombe (strain 972 / ATCC 24...	[more]
P0CT41	2.0e-38	31.91	Transposon Tf2-12 polyprotein OS=Schizosaccharomyces pombe (strain 972 / ATCC 24...	[more]
P0CT34	2.0e-38	31.91	Transposon Tf2-1 polyprotein OS=Schizosaccharomyces pombe (strain 972 / ATCC 248...	[more]

BLAST of Cmc12g0320951 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7U2V7	8.0e-160	98.21	Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold37...	[more]
A0A5D3BS67	8.0e-160	98.21	Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold50...	[more]
A0A5D3BHI1	8.0e-160	98.21	Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold11...	[more]
A0A5A7T1Y5	8.0e-160	98.21	Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold43...	[more]
A0A5A7UUL6	8.0e-160	98.21	Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold13...	[more]

BLAST of Cmc12g0320951 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description

InterPro

Analysis Name: InterPro Annotations of Melon (Charmono) v1.1
Date Performed: 2022-10-13


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR036397	Ribonuclease H superfamily	GENE3D	3.30.420.10		coord: 33..232 e-value: 1.1E-43 score: 150.9
None	No IPR available	PANTHER	PTHR45835	YALI0A06105P	coord: 6..184
IPR001584	Integrase, catalytic core	PROSITE	PS50994	INTEGRASE	coord: 29..194 score: 17.250114
IPR012337	Ribonuclease H-like superfamily	SUPERFAMILY	53098	Ribonuclease H-like	coord: 30..188

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cmc12g0320951.1	Cmc12g0320951.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0015074	DNA integration
molecular_function	GO:0003676	nucleic acid binding