Cmc12g0317571 (gene) Melon (Charmono) v1.1

Overview
NameCmc12g0317571
Typegene
OrganismCucumis melo L. var. cantalupensis cv. Charmono (Melon (Charmono) v1.1)
DescriptionDDE Tnp4 domain-containing protein
LocationCMiso1.1chr12: 2872787 .. 2875950 (+)
RNA-Seq ExpressionCmc12g0317571
SyntenyCmc12g0317571
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TTTGATAATTTTGGAATTAATGGAATTGGGAAGAGCCTTTTATGAAGACCTCCGCCGTCAGATTTGTCTCACAAAACAAAATCGCCTTTAGCTCATTCGCTAACCCAAAGCTTCTTGCTCTCTCTCATGGACTCCCACCTTTGATTGGACCTTTTCTTCTTTTAAAGTTGGTTTCGTTCAAAGGGCGGCCCCCGATCTTTCTCCACTGCATGTTCTAAAAAACCCAATTCCTGTTTTTACCAAAATTTCAAGAATCTGTGTGCGATTTTGTGGAATATTATGTATCCTCTTCGTCAATAACATTTTCTTTATTTTTGGGTTTTAATCACTCACTCGTTGTGGCTAATGGGTCCAATCAGAGGTTTGAGAAAGAAGAAGAAATTAGAGAGGAAGCTCGATTCCAATGGTACTGCTTCTGATTCTTCTGAAAAGGATGACGCTATAGATTGGTGGGATGATTTCTCCAAACGAACCAATGGTATTTCTTTCTTTTTTTCTTTTTAATATCTTGATTTTCTCTTTACTTCTAATCGAAGTTCTTCGATTGTAAGATCGGTTCTGTTTGACTATCGACTGTTTGGAACAAAAATGATTTGAACTTAGTTGACTTCTTATCTGGTGTGTAGAAAGAATTGACGGTACTGCATGCATTGGGAGGTTTGATAATTATGTTAGCTTCGAGTCATTGTATAGAAGGGCAAGACATTTTGTTGAGTTTCTATGAAGAAATATTATGTTATGTGATTCTGTTTATAAGAGTTTGAATGAGTCAATGCCGTGGCCTTGCTTCTGAGATTTTGATCTGTTTTCTGTGCTCGTAATGTATAGAGCTTGACAGGGGATCTCGTTTTGGGTTTGATGCGTTAGTGTTTAATTTGTGAAGGGATTGATTATGCTGTTTGACTGGTCTAGCTTATGACGTTACACCTTCATAAGCTGTATTTAGTATTCTTTGGTTCTTCATAAGCTGTATTTAGTATTCTTTGGTTGTCAAGAGCTTCTCGAACTTGTAACTAGTTGACCCTGATTCGACATTAGTAGTTTATAGATGATAGTGACGATATAGACCCAGAGCTATGGAACTTGAGTGTCATTCTCTTGTTGAAATGAAATGGCCTTATCTTTATTTTCATCTCTCCACAAATTTGAGTGCTAGTTCTTTGAATTACAAGGATGCTTCTATATACTAGGGTGGGGGTGTGTTCCTTCTTCCAACCTTTCGCTATGTATTGGTTGTCCTTCCTTCTTCAGGGTGATGACAATGTTCTCTGCCAACTACTACCCCTGAATGTATGGTTTTGTTATGAGTGAAGTTCAGACACAACAAGAATGACGCACATCTTGTTTTGGAATTCCTTCCTATTATCTTAGAGAATGAAGAGAATTTATGCTATTCCTCTATCGTATCTGATTTTAGCAATTAGATAATATAACTTCCATATGTTACTAAATTGTGTTTTGAAGTGTTGAGACAACTATAGTAGATTGGACTGATGTAGAGTCGAGAGTGGATTGGAGAGTTAGAAAATGTAGTTATTCAATTATTAGGCTTTATAACTCACATCAAATGATTTACATCCTCATTGCTTTGGCATTGACGTAATGTCATTAGATTTTTCTTCTGGTGTTTATCAGTTTCTCAAGTCTTCTACTTTACATATTTGGAAACCACTGAGAATCTATTGCCATTACACTACAAAGTGAAAAACTAATCATACTGGTCATGAATGTATCCAATGATTTGGGGATTAGCTCAGCCTTTTCTCCTCAAAATATTCTGGACCAATTTCAGTGTTTTAGTTTGAAAATTTTGGTTACACTTTCTTGGCCCCATTTCTATGCAGGTCTTCATTCTGCATCAAAAGGTTTGGATAGATTCAAATCCATTTTCAAGGTCTCCCGAAAGACTTTCGATTACATATGTTTGCTTGTCAAGGACGACATGACAGCTAAATCTGGTCATTTTACATTTTTGAACGGTAGGCCGTTGTCTTTATGTGATCAAGTAGCTGTAGCTTTAAGAAGGCTGGGGTCCGGTGAATCGTTAGTGACAATTGGTGATTCACTTGGATTGAACCACTCGACTGTGTCTCAAGTCACATGGCGATTTGTGGAGTCAATGGAAGAAAGGGGGCTTTGCCATCTTCATTGGCCTTCAAATGAAGTGGAAATGGCTCAAGTGAAATCAAAATTTGAGAAAATACAAGGACTTCCTAACTGTTGTGGTTCGATCGATACCACCCACATCACGATGTGTCTGCCTGCTTCGGATCCCACAAGTTTTGTGTGGCTTGATGACGAAAAAAACCACAGCATGGTTTTGCAAGTGATTGTAGATGCAGAAATGAGGTTTCGAGACATATTAACTGGATTGCCTGGGAAATTGTCGGATAGGTTAGTTTTCCAGAGTTCAAACTTCCACAAGCTTTGTGACAAGGGGGAGAGGCTGAATGGGAAGAGGTTAGAGCTTCCCGACAGATCGGAAATCCAAGAATATATAGTTGGAGATTCCGGCTATCCTTTACTTTCCTATCTTGTCACTCCCTATGATGGAAAAGAACTCTCAACATCAAAAGCTGAGTTCAATAAGCGACACACAGCGACTCGGTTGGTGGTTCAACAAGCACTAGCAATGTTGAAAGAACGGTGGAGGATCATTCAAGGAGTGATGTGGAGACCCGATAAACATCGGTTGCCGAGGATCATTCTAGTCTGCTGCTTACTTCATAACATTATCATTGATATCGGAGATGAAACAGAGGATGGTGATGTTCCTTTGTCTATCGAACATGACGTTGATTACAAACAACAGGTATGTGATGTTTTTGACTCAAAGGGTGCATATTTGAGAGAGAGATTGTCTTTGCTGTTCATATGAAACTGAGAGGCTTGGTTACCATATTTACTATTCTTTTACATTCTACAAGGTTTAAATTATGGGATTAAGCTTCTGTCACATGTACATATCATCAATTGATGCTTTAGTCATTTATTGGGGAGTGATTGATGAATTGATTCAGGTCCAATGATCTGAAATTTGTCTTTTGAACATTGAAAGAGGGAAATTACAGAACACACAAGTTATTAGTATATGAGCTGTGGTTTTTGTTCTTTTGTTGTGAACAGTATGGAAATATTTCAGCGTTACATACAAATTTCAACTT

mRNA sequence

TTTGATAATTTTGGAATTAATGGAATTGGGAAGAGCCTTTTATGAAGACCTCCGCCGTCAGATTTGTCTCACAAAACAAAATCGCCTTTAGCTCATTCGCTAACCCAAAGCTTCTTGCTCTCTCTCATGGACTCCCACCTTTGATTGGACCTTTTCTTCTTTTAAAGTTGGTTTCGTTCAAAGGGCGGCCCCCGATCTTTCTCCACTGCATGTTCTAAAAAACCCAATTCCTGTTTTTACCAAAATTTCAAGAATCTGTGTGCGATTTTGTGGAATATTATGTATCCTCTTCGTCAATAACATTTTCTTTATTTTTGGGTTTTAATCACTCACTCGTTGTGGCTAATGGGTCCAATCAGAGGTTTGAGAAAGAAGAAGAAATTAGAGAGGAAGCTCGATTCCAATGGTACTGCTTCTGATTCTTCTGAAAAGGATGACGCTATAGATTGGTGGGATGATTTCTCCAAACGAACCAATGGTCTTCATTCTGCATCAAAAGGTTTGGATAGATTCAAATCCATTTTCAAGGTCTCCCGAAAGACTTTCGATTACATATGTTTGCTTGTCAAGGACGACATGACAGCTAAATCTGGTCATTTTACATTTTTGAACGGTAGGCCGTTGTCTTTATGTGATCAAGTAGCTGTAGCTTTAAGAAGGCTGGGGTCCGGTGAATCGTTAGTGACAATTGGTGATTCACTTGGATTGAACCACTCGACTGTGTCTCAAGTCACATGGCGATTTGTGGAGTCAATGGAAGAAAGGGGGCTTTGCCATCTTCATTGGCCTTCAAATGAAGTGGAAATGGCTCAAGTGAAATCAAAATTTGAGAAAATACAAGGACTTCCTAACTGTTGTGGTTCGATCGATACCACCCACATCACGATGTGTCTGCCTGCTTCGGATCCCACAAGTTTTGTGTGGCTTGATGACGAAAAAAACCACAGCATGGTTTTGCAAGTGATTGTAGATGCAGAAATGAGGTTTCGAGACATATTAACTGGATTGCCTGGGAAATTGTCGGATAGGTTAGTTTTCCAGAGTTCAAACTTCCACAAGCTTTGTGACAAGGGGGAGAGGCTGAATGGGAAGAGGTTAGAGCTTCCCGACAGATCGGAAATCCAAGAATATATAGTTGGAGATTCCGGCTATCCTTTACTTTCCTATCTTGTCACTCCCTATGATGGAAAAGAACTCTCAACATCAAAAGCTGAGTTCAATAAGCGACACACAGCGACTCGGTTGGTGGTTCAACAAGCACTAGCAATGTTGAAAGAACGGTGGAGGATCATTCAAGGAGTGATGTGGAGACCCGATAAACATCGGTTGCCGAGGATCATTCTAGTCTGCTGCTTACTTCATAACATTATCATTGATATCGGAGATGAAACAGAGGATGGTGATGTTCCTTTGTCTATCGAACATGACGTTGATTACAAACAACAGGTATGTGATGTTTTTGACTCAAAGGGTGCATATTTGAGAGAGAGATTGTCTTTGCTGTTCATATGAAACTGAGAGGCTTGGTTACCATATTTACTATTCTTTTACATTCTACAAGGTTTAAATTATGGGATTAAGCTTCTGTCACATGTACATATCATCAATTGATGCTTTAGTCATTTATTGGGGAGTGATTGATGAATTGATTCAGGTCCAATGATCTGAAATTTGTCTTTTGAACATTGAAAGAGGGAAATTACAGAACACACAAGTTATTAGTATATGAGCTGTGGTTTTTGTTCTTTTGTTGTGAACAGTATGGAAATATTTCAGCGTTACATACAAATTTCAACTT

Coding sequence (CDS)

ATGGGTCCAATCAGAGGTTTGAGAAAGAAGAAGAAATTAGAGAGGAAGCTCGATTCCAATGGTACTGCTTCTGATTCTTCTGAAAAGGATGACGCTATAGATTGGTGGGATGATTTCTCCAAACGAACCAATGGTCTTCATTCTGCATCAAAAGGTTTGGATAGATTCAAATCCATTTTCAAGGTCTCCCGAAAGACTTTCGATTACATATGTTTGCTTGTCAAGGACGACATGACAGCTAAATCTGGTCATTTTACATTTTTGAACGGTAGGCCGTTGTCTTTATGTGATCAAGTAGCTGTAGCTTTAAGAAGGCTGGGGTCCGGTGAATCGTTAGTGACAATTGGTGATTCACTTGGATTGAACCACTCGACTGTGTCTCAAGTCACATGGCGATTTGTGGAGTCAATGGAAGAAAGGGGGCTTTGCCATCTTCATTGGCCTTCAAATGAAGTGGAAATGGCTCAAGTGAAATCAAAATTTGAGAAAATACAAGGACTTCCTAACTGTTGTGGTTCGATCGATACCACCCACATCACGATGTGTCTGCCTGCTTCGGATCCCACAAGTTTTGTGTGGCTTGATGACGAAAAAAACCACAGCATGGTTTTGCAAGTGATTGTAGATGCAGAAATGAGGTTTCGAGACATATTAACTGGATTGCCTGGGAAATTGTCGGATAGGTTAGTTTTCCAGAGTTCAAACTTCCACAAGCTTTGTGACAAGGGGGAGAGGCTGAATGGGAAGAGGTTAGAGCTTCCCGACAGATCGGAAATCCAAGAATATATAGTTGGAGATTCCGGCTATCCTTTACTTTCCTATCTTGTCACTCCCTATGATGGAAAAGAACTCTCAACATCAAAAGCTGAGTTCAATAAGCGACACACAGCGACTCGGTTGGTGGTTCAACAAGCACTAGCAATGTTGAAAGAACGGTGGAGGATCATTCAAGGAGTGATGTGGAGACCCGATAAACATCGGTTGCCGAGGATCATTCTAGTCTGCTGCTTACTTCATAACATTATCATTGATATCGGAGATGAAACAGAGGATGGTGATGTTCCTTTGTCTATCGAACATGACGTTGATTACAAACAACAGGTATGTGATGTTTTTGACTCAAAGGGTGCATATTTGAGAGAGAGATTGTCTTTGCTGTTCATATGA

Protein sequence

MGPIRGLRKKKKLERKLDSNGTASDSSEKDDAIDWWDDFSKRTNGLHSASKGLDRFKSIFKVSRKTFDYICLLVKDDMTAKSGHFTFLNGRPLSLCDQVAVALRRLGSGESLVTIGDSLGLNHSTVSQVTWRFVESMEERGLCHLHWPSNEVEMAQVKSKFEKIQGLPNCCGSIDTTHITMCLPASDPTSFVWLDDEKNHSMVLQVIVDAEMRFRDILTGLPGKLSDRLVFQSSNFHKLCDKGERLNGKRLELPDRSEIQEYIVGDSGYPLLSYLVTPYDGKELSTSKAEFNKRHTATRLVVQQALAMLKERWRIIQGVMWRPDKHRLPRIILVCCLLHNIIIDIGDETEDGDVPLSIEHDVDYKQQVCDVFDSKGAYLRERLSLLFI
Homology
BLAST of Cmc12g0317571 vs. NCBI nr
Match: KAA0033909.1 (putative nuclease HARBI1 [Cucumis melo var. makuwa])

HSP 1 Score: 794.7 bits (2051), Expect = 3.7e-226
Identity = 388/388 (100.00%), Postives = 388/388 (100.00%), Query Frame = 0

Query: 1   MGPIRGLRKKKKLERKLDSNGTASDSSEKDDAIDWWDDFSKRTNGLHSASKGLDRFKSIF 60
           MGPIRGLRKKKKLERKLDSNGTASDSSEKDDAIDWWDDFSKRTNGLHSASKGLDRFKSIF
Sbjct: 1   MGPIRGLRKKKKLERKLDSNGTASDSSEKDDAIDWWDDFSKRTNGLHSASKGLDRFKSIF 60

Query: 61  KVSRKTFDYICLLVKDDMTAKSGHFTFLNGRPLSLCDQVAVALRRLGSGESLVTIGDSLG 120
           KVSRKTFDYICLLVKDDMTAKSGHFTFLNGRPLSLCDQVAVALRRLGSGESLVTIGDSLG
Sbjct: 61  KVSRKTFDYICLLVKDDMTAKSGHFTFLNGRPLSLCDQVAVALRRLGSGESLVTIGDSLG 120

Query: 121 LNHSTVSQVTWRFVESMEERGLCHLHWPSNEVEMAQVKSKFEKIQGLPNCCGSIDTTHIT 180
           LNHSTVSQVTWRFVESMEERGLCHLHWPSNEVEMAQVKSKFEKIQGLPNCCGSIDTTHIT
Sbjct: 121 LNHSTVSQVTWRFVESMEERGLCHLHWPSNEVEMAQVKSKFEKIQGLPNCCGSIDTTHIT 180

Query: 181 MCLPASDPTSFVWLDDEKNHSMVLQVIVDAEMRFRDILTGLPGKLSDRLVFQSSNFHKLC 240
           MCLPASDPTSFVWLDDEKNHSMVLQVIVDAEMRFRDILTGLPGKLSDRLVFQSSNFHKLC
Sbjct: 181 MCLPASDPTSFVWLDDEKNHSMVLQVIVDAEMRFRDILTGLPGKLSDRLVFQSSNFHKLC 240

Query: 241 DKGERLNGKRLELPDRSEIQEYIVGDSGYPLLSYLVTPYDGKELSTSKAEFNKRHTATRL 300
           DKGERLNGKRLELPDRSEIQEYIVGDSGYPLLSYLVTPYDGKELSTSKAEFNKRHTATRL
Sbjct: 241 DKGERLNGKRLELPDRSEIQEYIVGDSGYPLLSYLVTPYDGKELSTSKAEFNKRHTATRL 300

Query: 301 VVQQALAMLKERWRIIQGVMWRPDKHRLPRIILVCCLLHNIIIDIGDETEDGDVPLSIEH 360
           VVQQALAMLKERWRIIQGVMWRPDKHRLPRIILVCCLLHNIIIDIGDETEDGDVPLSIEH
Sbjct: 301 VVQQALAMLKERWRIIQGVMWRPDKHRLPRIILVCCLLHNIIIDIGDETEDGDVPLSIEH 360

Query: 361 DVDYKQQVCDVFDSKGAYLRERLSLLFI 389
           DVDYKQQVCDVFDSKGAYLRERLSLLFI
Sbjct: 361 DVDYKQQVCDVFDSKGAYLRERLSLLFI 388

BLAST of Cmc12g0317571 vs. NCBI nr
Match: TYK01291.1 (putative nuclease HARBI1 [Cucumis melo var. makuwa])

HSP 1 Score: 790.0 bits (2039), Expect = 9.1e-225
Identity = 387/388 (99.74%), Postives = 387/388 (99.74%), Query Frame = 0

Query: 1   MGPIRGLRKKKKLERKLDSNGTASDSSEKDDAIDWWDDFSKRTNGLHSASKGLDRFKSIF 60
           MGPIRGLRKKKKLERKLDSNGTASDSSEKDDAIDWWDDFSKRTNGLHSASKGLDRFKSIF
Sbjct: 1   MGPIRGLRKKKKLERKLDSNGTASDSSEKDDAIDWWDDFSKRTNGLHSASKGLDRFKSIF 60

Query: 61  KVSRKTFDYICLLVKDDMTAKSGHFTFLNGRPLSLCDQVAVALRRLGSGESLVTIGDSLG 120
           KVSRKTFDYICLLVKDDMTAKSGHFTFLNGRPLSLCDQVAVALRRLGSGESLVTIGDSLG
Sbjct: 61  KVSRKTFDYICLLVKDDMTAKSGHFTFLNGRPLSLCDQVAVALRRLGSGESLVTIGDSLG 120

Query: 121 LNHSTVSQVTWRFVESMEERGLCHLHWPSNEVEMAQVKSKFEKIQGLPNCCGSIDTTHIT 180
           LNHSTVSQVTWRFVESMEERGL HLHWPSNEVEMAQVKSKFEKIQGLPNCCGSIDTTHIT
Sbjct: 121 LNHSTVSQVTWRFVESMEERGLRHLHWPSNEVEMAQVKSKFEKIQGLPNCCGSIDTTHIT 180

Query: 181 MCLPASDPTSFVWLDDEKNHSMVLQVIVDAEMRFRDILTGLPGKLSDRLVFQSSNFHKLC 240
           MCLPASDPTSFVWLDDEKNHSMVLQVIVDAEMRFRDILTGLPGKLSDRLVFQSSNFHKLC
Sbjct: 181 MCLPASDPTSFVWLDDEKNHSMVLQVIVDAEMRFRDILTGLPGKLSDRLVFQSSNFHKLC 240

Query: 241 DKGERLNGKRLELPDRSEIQEYIVGDSGYPLLSYLVTPYDGKELSTSKAEFNKRHTATRL 300
           DKGERLNGKRLELPDRSEIQEYIVGDSGYPLLSYLVTPYDGKELSTSKAEFNKRHTATRL
Sbjct: 241 DKGERLNGKRLELPDRSEIQEYIVGDSGYPLLSYLVTPYDGKELSTSKAEFNKRHTATRL 300

Query: 301 VVQQALAMLKERWRIIQGVMWRPDKHRLPRIILVCCLLHNIIIDIGDETEDGDVPLSIEH 360
           VVQQALAMLKERWRIIQGVMWRPDKHRLPRIILVCCLLHNIIIDIGDETEDGDVPLSIEH
Sbjct: 301 VVQQALAMLKERWRIIQGVMWRPDKHRLPRIILVCCLLHNIIIDIGDETEDGDVPLSIEH 360

Query: 361 DVDYKQQVCDVFDSKGAYLRERLSLLFI 389
           DVDYKQQVCDVFDSKGAYLRERLSLLFI
Sbjct: 361 DVDYKQQVCDVFDSKGAYLRERLSLLFI 388

BLAST of Cmc12g0317571 vs. NCBI nr
Match: XP_008457540.1 (PREDICTED: LOW QUALITY PROTEIN: putative nuclease HARBI1 [Cucumis melo])

HSP 1 Score: 790.0 bits (2039), Expect = 9.1e-225
Identity = 386/388 (99.48%), Postives = 386/388 (99.48%), Query Frame = 0

Query: 1   MGPIRGLRKKKKLERKLDSNGTASDSSEKDDAIDWWDDFSKRTNGLHSASKGLDRFKSIF 60
           MGPIRGLRKKKKLERKLDSNGTASDSSEKDDAIDWWDDFSKRTNGLHSASKGLDRFKSIF
Sbjct: 1   MGPIRGLRKKKKLERKLDSNGTASDSSEKDDAIDWWDDFSKRTNGLHSASKGLDRFKSIF 60

Query: 61  KVSRKTFDYICLLVKDDMTAKSGHFTFLNGRPLSLCDQVAVALRRLGSGESLVTIGDSLG 120
           KVSRKTFDYICLLVKDDMTAKSGHFTFLNGRPLSLCDQVAVALRRLGSGESLVTIGDSLG
Sbjct: 61  KVSRKTFDYICLLVKDDMTAKSGHFTFLNGRPLSLCDQVAVALRRLGSGESLVTIGDSLG 120

Query: 121 LNHSTVSQVTWRFVESMEERGLCHLHWPSNEVEMAQVKSKFEKIQGLPNCCGSIDTTHIT 180
           LNHSTVSQVTWRFVESMEERGLCHLHWPSNEVEMAQVKSKFEKIQGLPNCCGSIDTTHIT
Sbjct: 121 LNHSTVSQVTWRFVESMEERGLCHLHWPSNEVEMAQVKSKFEKIQGLPNCCGSIDTTHIT 180

Query: 181 MCLPASDPTSFVWLDDEKNHSMVLQVIVDAEMRFRDILTGLPGKLSDRLVFQSSNFHKLC 240
           MCLPASDPTSFVWLD  KNHSMVLQVIVDAEMRFRDILTGLPGKLSDRLVFQSSNFHKLC
Sbjct: 181 MCLPASDPTSFVWLDXRKNHSMVLQVIVDAEMRFRDILTGLPGKLSDRLVFQSSNFHKLC 240

Query: 241 DKGERLNGKRLELPDRSEIQEYIVGDSGYPLLSYLVTPYDGKELSTSKAEFNKRHTATRL 300
           DKGERLNGKRLELPDRSEIQEYIVGDSGYPLLSYLVTPYDGKELSTSKAEFNKRHTATRL
Sbjct: 241 DKGERLNGKRLELPDRSEIQEYIVGDSGYPLLSYLVTPYDGKELSTSKAEFNKRHTATRL 300

Query: 301 VVQQALAMLKERWRIIQGVMWRPDKHRLPRIILVCCLLHNIIIDIGDETEDGDVPLSIEH 360
           VVQQALAMLKERWRIIQGVMWRPDKHRLPRIILVCCLLHNIIIDIGDETEDGDVPLSIEH
Sbjct: 301 VVQQALAMLKERWRIIQGVMWRPDKHRLPRIILVCCLLHNIIIDIGDETEDGDVPLSIEH 360

Query: 361 DVDYKQQVCDVFDSKGAYLRERLSLLFI 389
           DVDYKQQVCDVFDSKGAYLRERLSLLFI
Sbjct: 361 DVDYKQQVCDVFDSKGAYLRERLSLLFI 388

BLAST of Cmc12g0317571 vs. NCBI nr
Match: XP_004149039.1 (protein ALP1-like [Cucumis sativus] >KGN65671.1 hypothetical protein Csa_019843 [Cucumis sativus])

HSP 1 Score: 764.2 bits (1972), Expect = 5.3e-217
Identity = 371/388 (95.62%), Postives = 380/388 (97.94%), Query Frame = 0

Query: 1   MGPIRGLRKKKKLERKLDSNGTASDSSEKDDAIDWWDDFSKRTNGLHSASKGLDRFKSIF 60
           MGPIRGLRKKKKLERKLD NGTASDSSEKDDAIDWWDDFSKRTNGLHSASKGLDRFKSIF
Sbjct: 1   MGPIRGLRKKKKLERKLDCNGTASDSSEKDDAIDWWDDFSKRTNGLHSASKGLDRFKSIF 60

Query: 61  KVSRKTFDYICLLVKDDMTAKSGHFTFLNGRPLSLCDQVAVALRRLGSGESLVTIGDSLG 120
           KVSRKTFDYICLLVKDDMTAKSGHFTFLNGRPLSLCDQVAVALRRLGSGESLVTIGDSLG
Sbjct: 61  KVSRKTFDYICLLVKDDMTAKSGHFTFLNGRPLSLCDQVAVALRRLGSGESLVTIGDSLG 120

Query: 121 LNHSTVSQVTWRFVESMEERGLCHLHWPSNEVEMAQVKSKFEKIQGLPNCCGSIDTTHIT 180
           LNHSTVSQVTWRFVESMEERGL HLHWPSNEVEMAQVKSKFEKIQGLPNCCGSIDTTHIT
Sbjct: 121 LNHSTVSQVTWRFVESMEERGLHHLHWPSNEVEMAQVKSKFEKIQGLPNCCGSIDTTHIT 180

Query: 181 MCLPASDPTSFVWLDDEKNHSMVLQVIVDAEMRFRDILTGLPGKLSDRLVFQSSNFHKLC 240
           MCLPASDPTS+VWLDD+KNHSMVLQVIVDAEMRFRDILTGLPGKLSD LVFQSSNFHKLC
Sbjct: 181 MCLPASDPTSYVWLDDKKNHSMVLQVIVDAEMRFRDILTGLPGKLSDWLVFQSSNFHKLC 240

Query: 241 DKGERLNGKRLELPDRSEIQEYIVGDSGYPLLSYLVTPYDGKELSTSKAEFNKRHTATRL 300
           DKGERLNGKR ELPDRSEI+EYI+GDSGYPLL YLVTPYDGKELSTSK EFNKRH  TRL
Sbjct: 241 DKGERLNGKRFELPDRSEIREYIIGDSGYPLLPYLVTPYDGKELSTSKTEFNKRHKETRL 300

Query: 301 VVQQALAMLKERWRIIQGVMWRPDKHRLPRIILVCCLLHNIIIDIGDETEDGDVPLSIEH 360
           VVQ+ALAMLKERWRIIQGVMWRPDKHRLPRIILVCCLLHNIIIDIGDETE+G+VPLSIEH
Sbjct: 301 VVQRALAMLKERWRIIQGVMWRPDKHRLPRIILVCCLLHNIIIDIGDETEEGEVPLSIEH 360

Query: 361 DVDYKQQVCDVFDSKGAYLRERLSLLFI 389
           DVDYKQQVCDVFDSKGAY+R+RLSLLFI
Sbjct: 361 DVDYKQQVCDVFDSKGAYVRDRLSLLFI 388

BLAST of Cmc12g0317571 vs. NCBI nr
Match: XP_038893506.1 (protein ALP1-like [Benincasa hispida])

HSP 1 Score: 751.5 bits (1939), Expect = 3.6e-213
Identity = 369/389 (94.86%), Postives = 378/389 (97.17%), Query Frame = 0

Query: 1   MGPIRGLRKKKKLERKLDSNGTASDSSEKDDAIDWWDDFSKRTNGLHSASKGLDRFKSIF 60
           MGPIRGLRKKKKLERKLDSNGTASDSSEKD+AIDWWDDFSKRTNGLHSASKGLDRFKSIF
Sbjct: 1   MGPIRGLRKKKKLERKLDSNGTASDSSEKDEAIDWWDDFSKRTNGLHSASKGLDRFKSIF 60

Query: 61  KVSRKTFDYICLLVKDDMTAKSGHFTFLNGRPLSLCDQVAVALRRLGSGESLVTIGDSLG 120
           KVSRKTFDYICLLVKDDMTAKSGHFTFLNGRPLSLCDQVAVALRRLGSGESLVTIGDSLG
Sbjct: 61  KVSRKTFDYICLLVKDDMTAKSGHFTFLNGRPLSLCDQVAVALRRLGSGESLVTIGDSLG 120

Query: 121 LNHSTVSQVTWRFVESMEERGLCHLHWPSNEVEMAQVKSKFEKIQGLPNCCGSIDTTHIT 180
           LNHSTVSQVTWRFVESMEERGL HLHWPSNEVEMAQVKSKFEKIQGLPNCCGSIDTTHIT
Sbjct: 121 LNHSTVSQVTWRFVESMEERGLRHLHWPSNEVEMAQVKSKFEKIQGLPNCCGSIDTTHIT 180

Query: 181 MCLPASDPTSFVWLDDEKNHSMVLQVIVDAEMRFRDILTGLPGKLSDRLVFQSSNFHKLC 240
           MCLPASD TS+VWLD+EKNHSMVLQVIVDAEMRFRDILTGLPGK+SD LVFQSSNFHKLC
Sbjct: 181 MCLPASDSTSYVWLDEEKNHSMVLQVIVDAEMRFRDILTGLPGKMSDWLVFQSSNFHKLC 240

Query: 241 DKGERLNGKRLELPDRSEIQEYIVGDSGYPLLSYLVTPYDGKELSTSKAEFNKRHTATRL 300
           DKGERLNGKRLEL DRSEI+EYI+GDSGYPLL YLVTPYDGKE STSKAEFNKRH  TRL
Sbjct: 241 DKGERLNGKRLELNDRSEIREYIIGDSGYPLLPYLVTPYDGKEHSTSKAEFNKRHKETRL 300

Query: 301 VVQQALAMLKERWRIIQGVMWRPDKHRLPRIILVCCLLHNIIIDIGDET-EDGDVPLSIE 360
           VVQ+ALAMLKERWRIIQGVMWRPDKHRLPRIILVCCLLHNIIIDIGD+T EDG VPLSIE
Sbjct: 301 VVQRALAMLKERWRIIQGVMWRPDKHRLPRIILVCCLLHNIIIDIGDDTEEDGGVPLSIE 360

Query: 361 HDVDYKQQVCDVFDSKGAYLRERLSLLFI 389
           HDVDYKQQVCDVFD KGAYLR+RLSLLFI
Sbjct: 361 HDVDYKQQVCDVFDPKGAYLRDRLSLLFI 389

BLAST of Cmc12g0317571 vs. ExPASy Swiss-Prot
Match: Q9M2U3 (Protein ALP1-like OS=Arabidopsis thaliana OX=3702 GN=At3g55350 PE=2 SV=1)

HSP 1 Score: 454.9 bits (1169), Expect = 9.1e-127
Identity = 224/403 (55.58%), Postives = 294/403 (72.95%), Query Frame = 0

Query: 1   MGPIRGLRKKKKLERKLDSN--------------GTASDSSEKDD-----AIDWWDDFSK 60
           MGPI+ ++KKK+ E+K+D N                A ++++ DD     ++DWWD FS+
Sbjct: 1   MGPIKTIKKKKRAEKKVDRNVLLAATAAATSASAAAALNNNDDDDDSSSQSLDWWDGFSR 60

Query: 61  RTNGLHSASKGLDRFKSIFKVSRKTFDYICLLVKDDMTAKSGHFTFLNGRPLSLCDQVAV 120
           R   ++  S     F+S+FK+SRKTFDYIC LVK D TAK  +F+  NG PLSL D+VAV
Sbjct: 61  R---IYGGSTDPKTFESVFKISRKTFDYICSLVKADFTAKPANFSDSNGNPLSLNDRVAV 120

Query: 121 ALRRLGSGESLVTIGDSLGLNHSTVSQVTWRFVESMEERGLCHLHWPSNEVEMAQVKSKF 180
           ALRRLGSGESL  IG++ G+N STVSQ+TWRFVESMEER + HL WPS   ++ ++KSKF
Sbjct: 121 ALRRLGSGESLSVIGETFGMNQSTVSQITWRFVESMEERAIHHLSWPS---KLDEIKSKF 180

Query: 181 EKIQGLPNCCGSIDTTHITMCLPASDPTSFVWLDDEKNHSMVLQVIVDAEMRFRDILTGL 240
           EKI GLPNCCG+ID THI M LPA +P++ VWLD EKN SM LQ +VD +MRF D++ G 
Sbjct: 181 EKISGLPNCCGAIDITHIVMNLPAVEPSNKVWLDGEKNFSMTLQAVVDPDMRFLDVIAGW 240

Query: 241 PGKLSDRLVFQSSNFHKLCDKGERLNGKRLELPDRSEIQEYIVGDSGYPLLSYLVTPYDG 300
           PG L+D +V ++S F+KL +KG+RLNG++L L +R+E++EYIVGDSG+PLL +L+TPY G
Sbjct: 241 PGSLNDDVVLKNSGFYKLVEKGKRLNGEKLPLSERTELREYIVGDSGFPLLPWLLTPYQG 300

Query: 301 KELSTSKAEFNKRHTATRLVVQQALAMLKERWRIIQGVMWRPDKHRLPRIILVCCLLHNI 360
           K  S  + EFNKRH+      Q AL+ LK+RWRII GVMW PD++RLPRII VCCLLHNI
Sbjct: 301 KPTSLPQTEFNKRHSEATKAAQMALSKLKDRWRIINGVMWMPDRNRLPRIIFVCCLLHNI 360

Query: 361 IIDIGDETEDGDVPLSIEHDVDYKQQVCDVFDSKGAYLRERLS 385
           IID+ D+T D D PLS +HD++Y+Q+ C + D   + LR+ LS
Sbjct: 361 IIDMEDQTLD-DQPLSQQHDMNYRQRSCKLADEASSVLRDELS 396

BLAST of Cmc12g0317571 vs. ExPASy Swiss-Prot
Match: Q94K49 (Protein ANTAGONIST OF LIKE HETEROCHROMATIN PROTEIN 1 OS=Arabidopsis thaliana OX=3702 GN=ALP1 PE=1 SV=1)

HSP 1 Score: 329.3 bits (843), Expect = 5.7e-89
Identity = 180/393 (45.80%), Postives = 243/393 (61.83%), Query Frame = 0

Query: 1   MGPIRGLRKKKKLERKLDSNGTASDSSEK---------DDAI--DWWDDFSKRTNGLHSA 60
           M P++  +KKK  ++ LD     + + EK          +AI  DWWD F  R +     
Sbjct: 1   MAPVK--QKKKNKKKPLDKAKKLAKNKEKKRVNAVPLDPEAIDCDWWDTFWLRNSSPSVP 60

Query: 61  SKGLDRFKSIFKVSRKTFDYICLLVKDDMTAK--SGHFTFLNGRPLSLCDQVAVALRRLG 120
           S     FK  F+ S+ TF YIC LV++D+ ++  SG    + GR LS+  QVA+ALRRL 
Sbjct: 61  SDEDYAFKHFFRASKTTFSYICSLVREDLISRPPSGLIN-IEGRLLSVEKQVAIALRRLA 120

Query: 121 SGESLVTIGDSLGLNHSTVSQVTWRFVESMEERGLCHLHWPSNEVEMAQVKSKFEKIQGL 180
           SG+S V++G + G+  STVSQVTWRF+E++EER   HL WP ++  + ++KSKFE++ GL
Sbjct: 121 SGDSQVSVGAAFGVGQSTVSQVTWRFIEALEERAKHHLRWPDSD-RIEEIKSKFEEMYGL 180

Query: 181 PNCCGSIDTTHITMCLPASDPTSFVWLDDEKNHSMVLQVIVDAEMRFRDILTGLPGKLSD 240
           PNCCG+IDTTHI M LPA    S  W D EKN+SM LQ + D EMRF +++TG PG ++ 
Sbjct: 181 PNCCGAIDTTHIIMTLPAVQ-ASDDWCDQEKNYSMFLQGVFDHEMRFLNMVTGWPGGMTV 240

Query: 241 RLVFQSSNFHKLCDKGERLNGKRLELPDRSEIQEYIVGDSGYPLLSYLVTPYDGKELSTS 300
             + + S F KLC+  + L+G    L   ++I+EY+VG   YPLL +L+TP+D    S S
Sbjct: 241 SKLLKFSGFFKLCENAQILDGNPKTLSQGAQIREYVVGGISYPLLPWLITPHDSDHPSDS 300

Query: 301 KAEFNKRHTATRLVVQQALAMLKERWRIIQGVMWRPDKHRLPRIILVCCLLHNIIIDIGD 360
              FN+RH   R V   A   LK  WRI+  VMWRPD+ +LP IILVCCLLHNIIID GD
Sbjct: 301 MVAFNERHEKVRSVAATAFQQLKGSWRILSKVMWRPDRRKLPSIILVCCLLHNIIIDCGD 360

Query: 361 ETEDGDVPLSIEHDVDYKQQVCDVFDSKGAYLR 381
             ++ DVPLS  HD  Y  + C   +  G+ LR
Sbjct: 361 YLQE-DVPLSGHHDSGYADRYCKQTEPLGSELR 387

BLAST of Cmc12g0317571 vs. ExPASy Swiss-Prot
Match: Q6AZB8 (Putative nuclease HARBI1 OS=Danio rerio OX=7955 GN=harbi1 PE=2 SV=1)

HSP 1 Score: 125.2 bits (313), Expect = 1.6e-27
Identity = 86/310 (27.74%), Postives = 150/310 (48.39%), Query Frame = 0

Query: 51  KGLDRFK----------SIFKVSRKTFDYICLLVKDDMTAKSGHFTFLNGRPLSLCDQVA 110
           K LDRF           + F   R+   Y+  L+KD +  ++        R +S   Q+ 
Sbjct: 20  KTLDRFDIETVSDDFLLNTFGFPREFIYYLVELLKDSLLRRT-----QRSRAISPDVQIL 79

Query: 111 VALRRLGSGESLVTIGDSLGLNHSTVSQVTWRFVESMEERGLCHLHWPSNEVEMAQVKSK 170
            AL    SG     +GD++G++ +++S+      +++ E+    + +  +E    Q K +
Sbjct: 80  AALGFYTSGSFQSKMGDAIGISQASMSRCVSNVTKALIEKAPEFIGFTRDEATKQQFKDE 139

Query: 171 FEKIQGLPNCCGSIDTTHITMCLPASDPTSFVWLDDEKNHSMVLQVIVDAEMRFRDILTG 230
           F +I G+PN  G +D  HI +  P +D +S+V  + +  HS+  Q++ DA        T 
Sbjct: 140 FYRIAGIPNVTGVVDCAHIAIKAPNADDSSYV--NKKGFHSINCQLVCDARGLLLSAETH 199

Query: 231 LPGKLSDRLVFQSSNFHKLCDKGERLNGKRLELPDRSEIQEYIVGDSGYPLLSYLVTPYD 290
            PG L+DR VF+ SN  KL ++ E            ++ + +++GD+ YPL  +L+TP  
Sbjct: 200 WPGSLTDRAVFKQSNVAKLFEEQE------------NDDEGWLLGDNRYPLKKWLMTPVQ 259

Query: 291 GKELSTSKAEFNKRHTATRLVVQQALAMLKERWRIIQG----VMWRPDKHRLPRIILVCC 347
             E S +   +N  HT T  +V +    ++ R+R + G    + + P+K     II  CC
Sbjct: 260 SPE-SPADYRYNLAHTTTHEIVDRTFRAIQTRFRCLDGAKGYLQYSPEK--CSHIIQACC 307

BLAST of Cmc12g0317571 vs. ExPASy Swiss-Prot
Match: Q8BR93 (Putative nuclease HARBI1 OS=Mus musculus OX=10090 GN=Harbi1 PE=2 SV=1)

HSP 1 Score: 121.3 bits (303), Expect = 2.4e-26
Identity = 81/299 (27.09%), Postives = 146/299 (48.83%), Query Frame = 0

Query: 91  RPLSLCDQVAVALRRLGSGESLVTIGDSLGLNHSTVSQVTWRFVESMEERGLCHLHWPSN 150
           R +S   Q+  AL    SG     +GD++G++ +++S+      E++ ER    +H+P +
Sbjct: 65  RAISPETQILAALGFYTSGSFQTRMGDAIGISQASMSRCVANVTEALVERASQFIHFPVD 124

Query: 151 EVEMAQVKSKFEKIQGLPNCCGSIDTTHITMCLPASDPTSFVWLDDEKNHSMVLQVIVDA 210
           E  +  +K +F  + G+P   G  D  H+ +  P ++  S+V  + +  HS+   V+ D 
Sbjct: 125 EAAVQSLKDEFYGLAGMPGVIGVADCIHVAIKAPNAEDLSYV--NRKGLHSLNCLVVCDI 184

Query: 211 EMRFRDILTGLPGKLSDRLVFQSSNFHKLCDKGERLNGKRLELPDRSEIQEYIVGDSGYP 270
                 + T  PG L D  V Q S+     + G         +P  S    +++GDS + 
Sbjct: 185 RGALMTVETSWPGSLQDCAVLQRSSLTSQFETG---------MPKDS----WLLGDSSFF 244

Query: 271 LLSYLVTPYDGKELSTSKAEFNKRHTATRLVVQQALAMLKERWRIIQG----VMWRPDKH 330
           L S+L+TP    E + ++  +N+ H+AT  V+++ L  L  R+R + G    + + P+K 
Sbjct: 245 LRSWLLTPLPIPE-TAAEYRYNRAHSATHSVIERTLQTLCCRFRCLDGSKGALQYSPEK- 304

Query: 331 RLPRIILVCCLLHNIIIDIGDETEDGDVPLSIEHDVDYKQQVCDVFDSKGAYLRERLSL 386
               IIL CC+LHNI +D G +     VP  I+   + + +  +  D +   +R+ L L
Sbjct: 305 -CSHIILACCVLHNISLDHGMDVWSSPVPGPIDQPPEGEDEHMESLDLEADRIRQELIL 345

BLAST of Cmc12g0317571 vs. ExPASy Swiss-Prot
Match: B0BN95 (Putative nuclease HARBI1 OS=Rattus norvegicus OX=10116 GN=Harbi1 PE=2 SV=1)

HSP 1 Score: 121.3 bits (303), Expect = 2.4e-26
Identity = 79/299 (26.42%), Postives = 148/299 (49.50%), Query Frame = 0

Query: 91  RPLSLCDQVAVALRRLGSGESLVTIGDSLGLNHSTVSQVTWRFVESMEERGLCHLHWPSN 150
           R +S   Q+  AL    SG     +GD++G++ +++S+      E++ ER    +H+P++
Sbjct: 65  RAISPETQILAALGFYTSGSFQTRMGDAIGISQASMSRCVANVTEALVERASQFIHFPAD 124

Query: 151 EVEMAQVKSKFEKIQGLPNCCGSIDTTHITMCLPASDPTSFVWLDDEKNHSMVLQVIVDA 210
           E  +  +K +F  + G+P   G++D  H+ +  P ++  S+V  + +  HS+   V+ D 
Sbjct: 125 EAAIQSLKDEFYGLAGMPGVIGAVDCIHVAIKAPNAEDLSYV--NRKGLHSLNCLVVCDI 184

Query: 211 EMRFRDILTGLPGKLSDRLVFQSSNFHKLCDKGERLNGKRLELPDRSEIQEYIVGDSGYP 270
                 + T  PG L D  V Q S+     + G         +P  S    +++GDS + 
Sbjct: 185 RGALMTVETSWPGSLQDCAVLQQSSLSSQFETG---------MPKDS----WLLGDSSFF 244

Query: 271 LLSYLVTPYDGKELSTSKAEFNKRHTATRLVVQQALAMLKERWRIIQG----VMWRPDKH 330
           L ++L+TP    E + ++  +N+ H+AT  V+++ L  L  R+R + G    + + P+K 
Sbjct: 245 LHTWLLTPLHIPE-TPAEYRYNRAHSATHSVIEKTLRTLCCRFRCLDGSKGALQYSPEKS 304

Query: 331 RLPRIILVCCLLHNIIIDIGDETEDGDVPLSIEHDVDYKQQVCDVFDSKGAYLRERLSL 386
               IIL CC+LHNI ++ G +     V   IE   + + +  +  D +   +R+ L L
Sbjct: 305 --SHIILACCVLHNISLEHGMDVWSSPVTGPIEQPPEGEDEQMESLDLEADRIRQELIL 345

BLAST of Cmc12g0317571 vs. ExPASy TrEMBL
Match: A0A5A7SXL0 (Putative nuclease HARBI1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold43059G001000 PE=3 SV=1)

HSP 1 Score: 794.7 bits (2051), Expect = 1.8e-226
Identity = 388/388 (100.00%), Postives = 388/388 (100.00%), Query Frame = 0

Query: 1   MGPIRGLRKKKKLERKLDSNGTASDSSEKDDAIDWWDDFSKRTNGLHSASKGLDRFKSIF 60
           MGPIRGLRKKKKLERKLDSNGTASDSSEKDDAIDWWDDFSKRTNGLHSASKGLDRFKSIF
Sbjct: 1   MGPIRGLRKKKKLERKLDSNGTASDSSEKDDAIDWWDDFSKRTNGLHSASKGLDRFKSIF 60

Query: 61  KVSRKTFDYICLLVKDDMTAKSGHFTFLNGRPLSLCDQVAVALRRLGSGESLVTIGDSLG 120
           KVSRKTFDYICLLVKDDMTAKSGHFTFLNGRPLSLCDQVAVALRRLGSGESLVTIGDSLG
Sbjct: 61  KVSRKTFDYICLLVKDDMTAKSGHFTFLNGRPLSLCDQVAVALRRLGSGESLVTIGDSLG 120

Query: 121 LNHSTVSQVTWRFVESMEERGLCHLHWPSNEVEMAQVKSKFEKIQGLPNCCGSIDTTHIT 180
           LNHSTVSQVTWRFVESMEERGLCHLHWPSNEVEMAQVKSKFEKIQGLPNCCGSIDTTHIT
Sbjct: 121 LNHSTVSQVTWRFVESMEERGLCHLHWPSNEVEMAQVKSKFEKIQGLPNCCGSIDTTHIT 180

Query: 181 MCLPASDPTSFVWLDDEKNHSMVLQVIVDAEMRFRDILTGLPGKLSDRLVFQSSNFHKLC 240
           MCLPASDPTSFVWLDDEKNHSMVLQVIVDAEMRFRDILTGLPGKLSDRLVFQSSNFHKLC
Sbjct: 181 MCLPASDPTSFVWLDDEKNHSMVLQVIVDAEMRFRDILTGLPGKLSDRLVFQSSNFHKLC 240

Query: 241 DKGERLNGKRLELPDRSEIQEYIVGDSGYPLLSYLVTPYDGKELSTSKAEFNKRHTATRL 300
           DKGERLNGKRLELPDRSEIQEYIVGDSGYPLLSYLVTPYDGKELSTSKAEFNKRHTATRL
Sbjct: 241 DKGERLNGKRLELPDRSEIQEYIVGDSGYPLLSYLVTPYDGKELSTSKAEFNKRHTATRL 300

Query: 301 VVQQALAMLKERWRIIQGVMWRPDKHRLPRIILVCCLLHNIIIDIGDETEDGDVPLSIEH 360
           VVQQALAMLKERWRIIQGVMWRPDKHRLPRIILVCCLLHNIIIDIGDETEDGDVPLSIEH
Sbjct: 301 VVQQALAMLKERWRIIQGVMWRPDKHRLPRIILVCCLLHNIIIDIGDETEDGDVPLSIEH 360

Query: 361 DVDYKQQVCDVFDSKGAYLRERLSLLFI 389
           DVDYKQQVCDVFDSKGAYLRERLSLLFI
Sbjct: 361 DVDYKQQVCDVFDSKGAYLRERLSLLFI 388

BLAST of Cmc12g0317571 vs. ExPASy TrEMBL
Match: A0A5D3BNB9 (Putative nuclease HARBI1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold49G00830 PE=3 SV=1)

HSP 1 Score: 790.0 bits (2039), Expect = 4.4e-225
Identity = 387/388 (99.74%), Postives = 387/388 (99.74%), Query Frame = 0

Query: 1   MGPIRGLRKKKKLERKLDSNGTASDSSEKDDAIDWWDDFSKRTNGLHSASKGLDRFKSIF 60
           MGPIRGLRKKKKLERKLDSNGTASDSSEKDDAIDWWDDFSKRTNGLHSASKGLDRFKSIF
Sbjct: 1   MGPIRGLRKKKKLERKLDSNGTASDSSEKDDAIDWWDDFSKRTNGLHSASKGLDRFKSIF 60

Query: 61  KVSRKTFDYICLLVKDDMTAKSGHFTFLNGRPLSLCDQVAVALRRLGSGESLVTIGDSLG 120
           KVSRKTFDYICLLVKDDMTAKSGHFTFLNGRPLSLCDQVAVALRRLGSGESLVTIGDSLG
Sbjct: 61  KVSRKTFDYICLLVKDDMTAKSGHFTFLNGRPLSLCDQVAVALRRLGSGESLVTIGDSLG 120

Query: 121 LNHSTVSQVTWRFVESMEERGLCHLHWPSNEVEMAQVKSKFEKIQGLPNCCGSIDTTHIT 180
           LNHSTVSQVTWRFVESMEERGL HLHWPSNEVEMAQVKSKFEKIQGLPNCCGSIDTTHIT
Sbjct: 121 LNHSTVSQVTWRFVESMEERGLRHLHWPSNEVEMAQVKSKFEKIQGLPNCCGSIDTTHIT 180

Query: 181 MCLPASDPTSFVWLDDEKNHSMVLQVIVDAEMRFRDILTGLPGKLSDRLVFQSSNFHKLC 240
           MCLPASDPTSFVWLDDEKNHSMVLQVIVDAEMRFRDILTGLPGKLSDRLVFQSSNFHKLC
Sbjct: 181 MCLPASDPTSFVWLDDEKNHSMVLQVIVDAEMRFRDILTGLPGKLSDRLVFQSSNFHKLC 240

Query: 241 DKGERLNGKRLELPDRSEIQEYIVGDSGYPLLSYLVTPYDGKELSTSKAEFNKRHTATRL 300
           DKGERLNGKRLELPDRSEIQEYIVGDSGYPLLSYLVTPYDGKELSTSKAEFNKRHTATRL
Sbjct: 241 DKGERLNGKRLELPDRSEIQEYIVGDSGYPLLSYLVTPYDGKELSTSKAEFNKRHTATRL 300

Query: 301 VVQQALAMLKERWRIIQGVMWRPDKHRLPRIILVCCLLHNIIIDIGDETEDGDVPLSIEH 360
           VVQQALAMLKERWRIIQGVMWRPDKHRLPRIILVCCLLHNIIIDIGDETEDGDVPLSIEH
Sbjct: 301 VVQQALAMLKERWRIIQGVMWRPDKHRLPRIILVCCLLHNIIIDIGDETEDGDVPLSIEH 360

Query: 361 DVDYKQQVCDVFDSKGAYLRERLSLLFI 389
           DVDYKQQVCDVFDSKGAYLRERLSLLFI
Sbjct: 361 DVDYKQQVCDVFDSKGAYLRERLSLLFI 388

BLAST of Cmc12g0317571 vs. ExPASy TrEMBL
Match: A0A1S3C6F2 (LOW QUALITY PROTEIN: putative nuclease HARBI1 OS=Cucumis melo OX=3656 GN=LOC103497206 PE=3 SV=1)

HSP 1 Score: 790.0 bits (2039), Expect = 4.4e-225
Identity = 386/388 (99.48%), Postives = 386/388 (99.48%), Query Frame = 0

Query: 1   MGPIRGLRKKKKLERKLDSNGTASDSSEKDDAIDWWDDFSKRTNGLHSASKGLDRFKSIF 60
           MGPIRGLRKKKKLERKLDSNGTASDSSEKDDAIDWWDDFSKRTNGLHSASKGLDRFKSIF
Sbjct: 1   MGPIRGLRKKKKLERKLDSNGTASDSSEKDDAIDWWDDFSKRTNGLHSASKGLDRFKSIF 60

Query: 61  KVSRKTFDYICLLVKDDMTAKSGHFTFLNGRPLSLCDQVAVALRRLGSGESLVTIGDSLG 120
           KVSRKTFDYICLLVKDDMTAKSGHFTFLNGRPLSLCDQVAVALRRLGSGESLVTIGDSLG
Sbjct: 61  KVSRKTFDYICLLVKDDMTAKSGHFTFLNGRPLSLCDQVAVALRRLGSGESLVTIGDSLG 120

Query: 121 LNHSTVSQVTWRFVESMEERGLCHLHWPSNEVEMAQVKSKFEKIQGLPNCCGSIDTTHIT 180
           LNHSTVSQVTWRFVESMEERGLCHLHWPSNEVEMAQVKSKFEKIQGLPNCCGSIDTTHIT
Sbjct: 121 LNHSTVSQVTWRFVESMEERGLCHLHWPSNEVEMAQVKSKFEKIQGLPNCCGSIDTTHIT 180

Query: 181 MCLPASDPTSFVWLDDEKNHSMVLQVIVDAEMRFRDILTGLPGKLSDRLVFQSSNFHKLC 240
           MCLPASDPTSFVWLD  KNHSMVLQVIVDAEMRFRDILTGLPGKLSDRLVFQSSNFHKLC
Sbjct: 181 MCLPASDPTSFVWLDXRKNHSMVLQVIVDAEMRFRDILTGLPGKLSDRLVFQSSNFHKLC 240

Query: 241 DKGERLNGKRLELPDRSEIQEYIVGDSGYPLLSYLVTPYDGKELSTSKAEFNKRHTATRL 300
           DKGERLNGKRLELPDRSEIQEYIVGDSGYPLLSYLVTPYDGKELSTSKAEFNKRHTATRL
Sbjct: 241 DKGERLNGKRLELPDRSEIQEYIVGDSGYPLLSYLVTPYDGKELSTSKAEFNKRHTATRL 300

Query: 301 VVQQALAMLKERWRIIQGVMWRPDKHRLPRIILVCCLLHNIIIDIGDETEDGDVPLSIEH 360
           VVQQALAMLKERWRIIQGVMWRPDKHRLPRIILVCCLLHNIIIDIGDETEDGDVPLSIEH
Sbjct: 301 VVQQALAMLKERWRIIQGVMWRPDKHRLPRIILVCCLLHNIIIDIGDETEDGDVPLSIEH 360

Query: 361 DVDYKQQVCDVFDSKGAYLRERLSLLFI 389
           DVDYKQQVCDVFDSKGAYLRERLSLLFI
Sbjct: 361 DVDYKQQVCDVFDSKGAYLRERLSLLFI 388

BLAST of Cmc12g0317571 vs. ExPASy TrEMBL
Match: A0A0A0M0C2 (DDE Tnp4 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G481730 PE=3 SV=1)

HSP 1 Score: 764.2 bits (1972), Expect = 2.6e-217
Identity = 371/388 (95.62%), Postives = 380/388 (97.94%), Query Frame = 0

Query: 1   MGPIRGLRKKKKLERKLDSNGTASDSSEKDDAIDWWDDFSKRTNGLHSASKGLDRFKSIF 60
           MGPIRGLRKKKKLERKLD NGTASDSSEKDDAIDWWDDFSKRTNGLHSASKGLDRFKSIF
Sbjct: 1   MGPIRGLRKKKKLERKLDCNGTASDSSEKDDAIDWWDDFSKRTNGLHSASKGLDRFKSIF 60

Query: 61  KVSRKTFDYICLLVKDDMTAKSGHFTFLNGRPLSLCDQVAVALRRLGSGESLVTIGDSLG 120
           KVSRKTFDYICLLVKDDMTAKSGHFTFLNGRPLSLCDQVAVALRRLGSGESLVTIGDSLG
Sbjct: 61  KVSRKTFDYICLLVKDDMTAKSGHFTFLNGRPLSLCDQVAVALRRLGSGESLVTIGDSLG 120

Query: 121 LNHSTVSQVTWRFVESMEERGLCHLHWPSNEVEMAQVKSKFEKIQGLPNCCGSIDTTHIT 180
           LNHSTVSQVTWRFVESMEERGL HLHWPSNEVEMAQVKSKFEKIQGLPNCCGSIDTTHIT
Sbjct: 121 LNHSTVSQVTWRFVESMEERGLHHLHWPSNEVEMAQVKSKFEKIQGLPNCCGSIDTTHIT 180

Query: 181 MCLPASDPTSFVWLDDEKNHSMVLQVIVDAEMRFRDILTGLPGKLSDRLVFQSSNFHKLC 240
           MCLPASDPTS+VWLDD+KNHSMVLQVIVDAEMRFRDILTGLPGKLSD LVFQSSNFHKLC
Sbjct: 181 MCLPASDPTSYVWLDDKKNHSMVLQVIVDAEMRFRDILTGLPGKLSDWLVFQSSNFHKLC 240

Query: 241 DKGERLNGKRLELPDRSEIQEYIVGDSGYPLLSYLVTPYDGKELSTSKAEFNKRHTATRL 300
           DKGERLNGKR ELPDRSEI+EYI+GDSGYPLL YLVTPYDGKELSTSK EFNKRH  TRL
Sbjct: 241 DKGERLNGKRFELPDRSEIREYIIGDSGYPLLPYLVTPYDGKELSTSKTEFNKRHKETRL 300

Query: 301 VVQQALAMLKERWRIIQGVMWRPDKHRLPRIILVCCLLHNIIIDIGDETEDGDVPLSIEH 360
           VVQ+ALAMLKERWRIIQGVMWRPDKHRLPRIILVCCLLHNIIIDIGDETE+G+VPLSIEH
Sbjct: 301 VVQRALAMLKERWRIIQGVMWRPDKHRLPRIILVCCLLHNIIIDIGDETEEGEVPLSIEH 360

Query: 361 DVDYKQQVCDVFDSKGAYLRERLSLLFI 389
           DVDYKQQVCDVFDSKGAY+R+RLSLLFI
Sbjct: 361 DVDYKQQVCDVFDSKGAYVRDRLSLLFI 388

BLAST of Cmc12g0317571 vs. ExPASy TrEMBL
Match: A0A6J1K643 (protein ALP1-like OS=Cucurbita maxima OX=3661 GN=LOC111492660 PE=3 SV=1)

HSP 1 Score: 691.4 bits (1783), Expect = 2.1e-195
Identity = 339/389 (87.15%), Postives = 358/389 (92.03%), Query Frame = 0

Query: 1   MGPIRGLRKKKKLERKLDSN-GTASDSSEKDDAIDWWDDFSKRTNGLHSASKGLDRFKSI 60
           MGPIRG RKKKKLERKLD+N  TASDSSEKDDA+DWWDDFS+RT GLHS  +GLD FKSI
Sbjct: 1   MGPIRGSRKKKKLERKLDANASTASDSSEKDDALDWWDDFSRRTIGLHSELEGLDGFKSI 60

Query: 61  FKVSRKTFDYICLLVKDDMTAKSGHFTFLNGRPLSLCDQVAVALRRLGSGESLVTIGDSL 120
           FKVSRKTFDYICLLVKDDMTAKS +F FLNGRPLSL DQVAVALRRLGSG+SLVTIG S 
Sbjct: 61  FKVSRKTFDYICLLVKDDMTAKSSNFMFLNGRPLSLYDQVAVALRRLGSGDSLVTIGYSF 120

Query: 121 GLNHSTVSQVTWRFVESMEERGLCHLHWPSNEVEMAQVKSKFEKIQGLPNCCGSIDTTHI 180
           GLNHSTVSQVTWRFVESME RGL HLHWPS E EMAQVK KFEKIQGLPNCCGSIDTTHI
Sbjct: 121 GLNHSTVSQVTWRFVESMEVRGLRHLHWPSTEEEMAQVKLKFEKIQGLPNCCGSIDTTHI 180

Query: 181 TMCLPASDPTSFVWLDDEKNHSMVLQVIVDAEMRFRDILTGLPGKLSDRLVFQSSNFHKL 240
           TMCLP  DPTS VWLD EKNHSMVLQVIVDAEMRFRDI+TGLPGK+SD LVFQSSNFHKL
Sbjct: 181 TMCLPVLDPTSNVWLDAEKNHSMVLQVIVDAEMRFRDIITGLPGKMSDWLVFQSSNFHKL 240

Query: 241 CDKGERLNGKRLELPDRSEIQEYIVGDSGYPLLSYLVTPYDGKELSTSKAEFNKRHTATR 300
           C+KGERLNGKRLE  +RSEI+EYI+GDSGYPLL YLVTPYDGKEL  SKAEFNKRHT TR
Sbjct: 241 CEKGERLNGKRLEFINRSEIREYIIGDSGYPLLPYLVTPYDGKELQPSKAEFNKRHTETR 300

Query: 301 LVVQQALAMLKERWRIIQGVMWRPDKHRLPRIILVCCLLHNIIIDIGDETEDGDVPLSIE 360
           LVVQ+ALA LKERWRIIQGVMWRPDKHRLPRIILVCCLLHNIIID+GDE EDG+VP+SIE
Sbjct: 301 LVVQRALASLKERWRIIQGVMWRPDKHRLPRIILVCCLLHNIIIDVGDEMEDGNVPMSIE 360

Query: 361 HDVDYKQQVCDVFDSKGAYLRERLSLLFI 389
           HD DYKQQ+CDV+DSKGAYLRE+LSLLFI
Sbjct: 361 HDADYKQQICDVYDSKGAYLREKLSLLFI 389

BLAST of Cmc12g0317571 vs. TAIR 10
Match: AT3G55350.1 (PIF / Ping-Pong family of plant transposases )

HSP 1 Score: 454.9 bits (1169), Expect = 6.4e-128
Identity = 224/403 (55.58%), Postives = 294/403 (72.95%), Query Frame = 0

Query: 1   MGPIRGLRKKKKLERKLDSN--------------GTASDSSEKDD-----AIDWWDDFSK 60
           MGPI+ ++KKK+ E+K+D N                A ++++ DD     ++DWWD FS+
Sbjct: 1   MGPIKTIKKKKRAEKKVDRNVLLAATAAATSASAAAALNNNDDDDDSSSQSLDWWDGFSR 60

Query: 61  RTNGLHSASKGLDRFKSIFKVSRKTFDYICLLVKDDMTAKSGHFTFLNGRPLSLCDQVAV 120
           R   ++  S     F+S+FK+SRKTFDYIC LVK D TAK  +F+  NG PLSL D+VAV
Sbjct: 61  R---IYGGSTDPKTFESVFKISRKTFDYICSLVKADFTAKPANFSDSNGNPLSLNDRVAV 120

Query: 121 ALRRLGSGESLVTIGDSLGLNHSTVSQVTWRFVESMEERGLCHLHWPSNEVEMAQVKSKF 180
           ALRRLGSGESL  IG++ G+N STVSQ+TWRFVESMEER + HL WPS   ++ ++KSKF
Sbjct: 121 ALRRLGSGESLSVIGETFGMNQSTVSQITWRFVESMEERAIHHLSWPS---KLDEIKSKF 180

Query: 181 EKIQGLPNCCGSIDTTHITMCLPASDPTSFVWLDDEKNHSMVLQVIVDAEMRFRDILTGL 240
           EKI GLPNCCG+ID THI M LPA +P++ VWLD EKN SM LQ +VD +MRF D++ G 
Sbjct: 181 EKISGLPNCCGAIDITHIVMNLPAVEPSNKVWLDGEKNFSMTLQAVVDPDMRFLDVIAGW 240

Query: 241 PGKLSDRLVFQSSNFHKLCDKGERLNGKRLELPDRSEIQEYIVGDSGYPLLSYLVTPYDG 300
           PG L+D +V ++S F+KL +KG+RLNG++L L +R+E++EYIVGDSG+PLL +L+TPY G
Sbjct: 241 PGSLNDDVVLKNSGFYKLVEKGKRLNGEKLPLSERTELREYIVGDSGFPLLPWLLTPYQG 300

Query: 301 KELSTSKAEFNKRHTATRLVVQQALAMLKERWRIIQGVMWRPDKHRLPRIILVCCLLHNI 360
           K  S  + EFNKRH+      Q AL+ LK+RWRII GVMW PD++RLPRII VCCLLHNI
Sbjct: 301 KPTSLPQTEFNKRHSEATKAAQMALSKLKDRWRIINGVMWMPDRNRLPRIIFVCCLLHNI 360

Query: 361 IIDIGDETEDGDVPLSIEHDVDYKQQVCDVFDSKGAYLRERLS 385
           IID+ D+T D D PLS +HD++Y+Q+ C + D   + LR+ LS
Sbjct: 361 IIDMEDQTLD-DQPLSQQHDMNYRQRSCKLADEASSVLRDELS 396

BLAST of Cmc12g0317571 vs. TAIR 10
Match: AT3G63270.1 (CONTAINS InterPro DOMAIN/s: Putative harbinger transposase-derived nuclease (InterPro:IPR006912); BEST Arabidopsis thaliana protein match is: PIF / Ping-Pong family of plant transposases (TAIR:AT3G55350.1); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink). )

HSP 1 Score: 329.3 bits (843), Expect = 4.1e-90
Identity = 180/393 (45.80%), Postives = 243/393 (61.83%), Query Frame = 0

Query: 1   MGPIRGLRKKKKLERKLDSNGTASDSSEK---------DDAI--DWWDDFSKRTNGLHSA 60
           M P++  +KKK  ++ LD     + + EK          +AI  DWWD F  R +     
Sbjct: 1   MAPVK--QKKKNKKKPLDKAKKLAKNKEKKRVNAVPLDPEAIDCDWWDTFWLRNSSPSVP 60

Query: 61  SKGLDRFKSIFKVSRKTFDYICLLVKDDMTAK--SGHFTFLNGRPLSLCDQVAVALRRLG 120
           S     FK  F+ S+ TF YIC LV++D+ ++  SG    + GR LS+  QVA+ALRRL 
Sbjct: 61  SDEDYAFKHFFRASKTTFSYICSLVREDLISRPPSGLIN-IEGRLLSVEKQVAIALRRLA 120

Query: 121 SGESLVTIGDSLGLNHSTVSQVTWRFVESMEERGLCHLHWPSNEVEMAQVKSKFEKIQGL 180
           SG+S V++G + G+  STVSQVTWRF+E++EER   HL WP ++  + ++KSKFE++ GL
Sbjct: 121 SGDSQVSVGAAFGVGQSTVSQVTWRFIEALEERAKHHLRWPDSD-RIEEIKSKFEEMYGL 180

Query: 181 PNCCGSIDTTHITMCLPASDPTSFVWLDDEKNHSMVLQVIVDAEMRFRDILTGLPGKLSD 240
           PNCCG+IDTTHI M LPA    S  W D EKN+SM LQ + D EMRF +++TG PG ++ 
Sbjct: 181 PNCCGAIDTTHIIMTLPAVQ-ASDDWCDQEKNYSMFLQGVFDHEMRFLNMVTGWPGGMTV 240

Query: 241 RLVFQSSNFHKLCDKGERLNGKRLELPDRSEIQEYIVGDSGYPLLSYLVTPYDGKELSTS 300
             + + S F KLC+  + L+G    L   ++I+EY+VG   YPLL +L+TP+D    S S
Sbjct: 241 SKLLKFSGFFKLCENAQILDGNPKTLSQGAQIREYVVGGISYPLLPWLITPHDSDHPSDS 300

Query: 301 KAEFNKRHTATRLVVQQALAMLKERWRIIQGVMWRPDKHRLPRIILVCCLLHNIIIDIGD 360
              FN+RH   R V   A   LK  WRI+  VMWRPD+ +LP IILVCCLLHNIIID GD
Sbjct: 301 MVAFNERHEKVRSVAATAFQQLKGSWRILSKVMWRPDRRKLPSIILVCCLLHNIIIDCGD 360

Query: 361 ETEDGDVPLSIEHDVDYKQQVCDVFDSKGAYLR 381
             ++ DVPLS  HD  Y  + C   +  G+ LR
Sbjct: 361 YLQE-DVPLSGHHDSGYADRYCKQTEPLGSELR 387

BLAST of Cmc12g0317571 vs. TAIR 10
Match: AT5G12010.1 (unknown protein; INVOLVED IN: response to salt stress; LOCATED IN: chloroplast, plasma membrane, membrane; EXPRESSED IN: 23 plant structures; EXPRESSED DURING: 13 growth stages; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT4G29780.1); Has 1807 Blast hits to 1807 proteins in 277 species: Archae - 0; Bacteria - 0; Metazoa - 736; Fungi - 347; Plants - 385; Viruses - 0; Other Eukaryotes - 339 (source: NCBI BLink). )

HSP 1 Score: 130.6 bits (327), Expect = 2.8e-30
Identity = 82/319 (25.71%), Postives = 154/319 (48.28%), Query Frame = 0

Query: 29  KDDAIDWWDDFSKRTNGLHSASKGLDRFKSIFKVSRKTFDYICLLVKDDMTAKSGHFTFL 88
           KD +  WW++ S+            + FK  F++S+ TF+ IC    D++ +        
Sbjct: 155 KDRSRAWWEECSR-------LDYPEEDFKKAFRMSKSTFELIC----DELNSAVAKEDTA 214

Query: 89  NGRPLSLCDQVAVALRRLGSGESLVTIGDSLGLNHSTVSQVTWRFVESMEERGL-CHLHW 148
               + +  +VAV + RL +GE L  +    GL  ST  ++     +++++  +  +L W
Sbjct: 215 LRNAIPVRQRVAVCIWRLATGEPLRLVSKKFGLGISTCHKLVLEVCKAIKDVLMPKYLQW 274

Query: 149 PSNEVEMAQVKSKFEKIQGLPNCCGSIDTTHITMCLPASDPTSF-----VWLDDEKNHSM 208
           P +E  +  ++ +FE + G+PN  GS+ TTHI +  P     S+        + + ++S+
Sbjct: 275 PDDE-SLRNIRERFESVSGIPNVVGSMYTTHIPIIAPKISVASYFNKRHTERNQKTSYSI 334

Query: 209 VLQVIVDAEMRFRDILTGLPGKLSDRLVFQSSNFHKLCDKGERLNGKRLELPDRSEIQEY 268
            +Q +V+ +  F D+  G PG + D  V + S  ++  + G  L G             +
Sbjct: 335 TIQAVVNPKGVFTDLCIGWPGSMPDDKVLEKSLLYQRANNGGLLKGM------------W 394

Query: 269 IVGDSGYPLLSYLVTPYDGKELSTSKAEFNKRHTATRLVVQQALAMLKERWRIIQGVMWR 328
           + G  G+PLL +++ PY  + L+ ++  FN++ +  + V ++A   LK RW  +Q     
Sbjct: 395 VAGGPGHPLLDWVLVPYTQQNLTWTQHAFNEKMSEVQGVAKEAFGRLKGRWACLQ-KRTE 448

Query: 329 PDKHRLPRIILVCCLLHNI 342
                LP ++  CC+LHNI
Sbjct: 455 VKLQDLPTVLGACCVLHNI 448

BLAST of Cmc12g0317571 vs. TAIR 10
Match: AT4G29780.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT5G12010.1); Has 945 Blast hits to 944 proteins in 87 species: Archae - 0; Bacteria - 0; Metazoa - 519; Fungi - 43; Plants - 365; Viruses - 0; Other Eukaryotes - 18 (source: NCBI BLink). )

HSP 1 Score: 122.5 bits (306), Expect = 7.6e-28
Identity = 91/319 (28.53%), Postives = 151/319 (47.34%), Query Frame = 0

Query: 29  KDDAIDWWDDFSKRTNGLHSASKGLDRFKSIFKVSRKTFDYICLLVKDDMTAKSGHFTFL 88
           K+   DWWD  S+            D F+  F++S+ TF+ IC  +   +T K+   T L
Sbjct: 193 KERTTDWWDRVSR-------PDFPEDEFRREFRMSKSTFNLICEELDTTVTKKN---TML 252

Query: 89  NGRPLSLCDQVAVALRRLGSGESLVTIGDSLGLNHSTVSQVTWRFVESMEERGL-CHLHW 148
               +    +V V + RL +G  L  + +  GL  ST  ++      ++ +  +  +L W
Sbjct: 253 RD-AIPAPKRVGVCVWRLATGAPLRHVSERFGLGISTCHKLVIEVCRAIYDVLMPKYLLW 312

Query: 149 PSNEVEMAQVKSKFEKIQGLPNCCGSIDTTHITMCLPASDPTSF-----VWLDDEKNHSM 208
           PS+  E+   K+KFE +  +PN  GSI TTHI +  P     ++        + + ++S+
Sbjct: 313 PSDS-EINSTKAKFESVHKIPNVVGSIYTTHIPIIAPKVHVAAYFNKRHTERNQKTSYSI 372

Query: 209 VLQVIVDAEMRFRDILTGLPGKLSDRLVFQSSNFHKLCDKGERLNGKRLELPDRSEIQEY 268
            +Q +V+A+  F D+  G PG L+D  + + S+         R    R  L D      +
Sbjct: 373 TVQGVVNADGIFTDVCIGNPGSLTDDQILEKSSL-------SRQRAARGMLRD-----SW 432

Query: 269 IVGDSGYPLLSYLVTPYDGKELSTSKAEFNKRHTATRLVVQQALAMLKERWRIIQGVMWR 328
           IVG+SG+PL  YL+ PY  + L+ ++  FN+     + +   A   LK RW  +Q     
Sbjct: 433 IVGNSGFPLTDYLLVPYTRQNLTWTQHAFNESIGEIQGIATAAFERLKGRWACLQ-KRTE 486

Query: 329 PDKHRLPRIILVCCLLHNI 342
                LP ++  CC+LHNI
Sbjct: 493 VKLQDLPYVLGACCVLHNI 486

BLAST of Cmc12g0317571 vs. TAIR 10
Match: AT3G19120.1 (PIF / Ping-Pong family of plant transposases )

HSP 1 Score: 102.8 bits (255), Expect = 6.2e-22
Identity = 82/296 (27.70%), Postives = 138/296 (46.62%), Query Frame = 0

Query: 55  RFKSIFKVSRKTFDYICLLVKDDMTAKSGHFTFLNGRPLSLCDQVAVA--LRRLGSGESL 114
           R++S++ +S   F  +   +K  +TA +          LSL    AVA  L RL  G S 
Sbjct: 116 RWRSLYGLSYPVFITVVDKLKPFITASN----------LSLPADYAVAMVLSRLAHGCSA 175

Query: 115 VTIGDSLGLNHSTVSQVTWRFVESMEERGLC------HLHWPSNEVEMAQVKSKFEKIQG 174
            T+     L+   +S++T     +M  R L        +  P  +  + +    FE++  
Sbjct: 176 KTLASRYSLDPYLISKIT-----NMVTRLLATKLYPEFIKIPVGKRRLIETTQGFEELTS 235

Query: 175 LPNCCGSIDTTHITMCLPASDPTSFVWLDDEKNHSMVLQVIVDAEMRFRDILTGLPGKLS 234
           LPN CG+ID+T + +          ++       +++LQV+ D +  F D+    PG   
Sbjct: 236 LPNICGAIDSTPVKLRRRTKLNPRNIYGCKYGYDAVLLQVVADHKKIFWDVCVKAPGGED 295

Query: 235 DRLVFQSSNFHKLCDKGERLNGKRLELPDRSEIQEYIVGDSGYPLLSYLVTPYDGKELST 294
           D   F+ S  +K    G+ +  K + +     ++ YIVGD  YPLLS+L+TP+      T
Sbjct: 296 DSSHFRDSLLYKRLTSGDIVWEKVINIRGH-HVRPYIVGDWCYPLLSFLMTPFSPNGSGT 355

Query: 295 SKAE-FNKRHTATRLVVQQALAMLKERWRIIQGVMWRPDKHRLPRIILVCCLLHNI 342
                F+      R VV +A+ +LK RW+I+Q +      +  P+ I+ CC+LHN+
Sbjct: 356 PPENLFDGMLMKGRSVVVEAIGLLKARWKILQSL--NVGVNHAPQTIVACCVLHNL 393

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAA0033909.13.7e-226100.00putative nuclease HARBI1 [Cucumis melo var. makuwa][more]
TYK01291.19.1e-22599.74putative nuclease HARBI1 [Cucumis melo var. makuwa][more]
XP_008457540.19.1e-22599.48PREDICTED: LOW QUALITY PROTEIN: putative nuclease HARBI1 [Cucumis melo][more]
XP_004149039.15.3e-21795.62protein ALP1-like [Cucumis sativus] >KGN65671.1 hypothetical protein Csa_019843 ... [more]
XP_038893506.13.6e-21394.86protein ALP1-like [Benincasa hispida][more]
Match NameE-valueIdentityDescription
Q9M2U39.1e-12755.58Protein ALP1-like OS=Arabidopsis thaliana OX=3702 GN=At3g55350 PE=2 SV=1[more]
Q94K495.7e-8945.80Protein ANTAGONIST OF LIKE HETEROCHROMATIN PROTEIN 1 OS=Arabidopsis thaliana OX=... [more]
Q6AZB81.6e-2727.74Putative nuclease HARBI1 OS=Danio rerio OX=7955 GN=harbi1 PE=2 SV=1[more]
Q8BR932.4e-2627.09Putative nuclease HARBI1 OS=Mus musculus OX=10090 GN=Harbi1 PE=2 SV=1[more]
B0BN952.4e-2626.42Putative nuclease HARBI1 OS=Rattus norvegicus OX=10116 GN=Harbi1 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A5A7SXL01.8e-226100.00Putative nuclease HARBI1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffol... [more]
A0A5D3BNB94.4e-22599.74Putative nuclease HARBI1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffol... [more]
A0A1S3C6F24.4e-22599.48LOW QUALITY PROTEIN: putative nuclease HARBI1 OS=Cucumis melo OX=3656 GN=LOC1034... [more]
A0A0A0M0C22.6e-21795.62DDE Tnp4 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G481730 PE... [more]
A0A6J1K6432.1e-19587.15protein ALP1-like OS=Cucurbita maxima OX=3661 GN=LOC111492660 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT3G55350.16.4e-12855.58PIF / Ping-Pong family of plant transposases [more]
AT3G63270.14.1e-9045.80CONTAINS InterPro DOMAIN/s: Putative harbinger transposase-derived nuclease (Int... [more]
AT5G12010.12.8e-3025.71unknown protein; INVOLVED IN: response to salt stress; LOCATED IN: chloroplast, ... [more]
AT4G29780.17.6e-2828.53unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TA... [more]
AT3G19120.16.2e-2227.70PIF / Ping-Pong family of plant transposases [more]
InterPro
Analysis Name: InterPro Annotations of Melon (Charmono) v1.1
Date Performed: 2022-10-13
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR027806Harbinger transposase-derived nuclease domainPFAMPF13359DDE_Tnp_4coord: 174..340
e-value: 1.1E-28
score: 99.9
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..29
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 13..29
NoneNo IPR availablePANTHERPTHR22930UNCHARACTERIZEDcoord: 1..386
NoneNo IPR availablePANTHERPTHR22930:SF194NUCLEASE HARBI1 ISOFORM X1-RELATEDcoord: 1..386

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cmc12g0317571.1Cmc12g0317571.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0005634 nucleus
molecular_function GO:0016787 hydrolase activity
molecular_function GO:0046872 metal ion binding