Homology
BLAST of Cmc12g0317571 vs. NCBI nr
Match:
KAA0033909.1 (putative nuclease HARBI1 [Cucumis melo var. makuwa])
HSP 1 Score: 794.7 bits (2051), Expect = 3.7e-226
Identity = 388/388 (100.00%), Postives = 388/388 (100.00%), Query Frame = 0
Query: 1 MGPIRGLRKKKKLERKLDSNGTASDSSEKDDAIDWWDDFSKRTNGLHSASKGLDRFKSIF 60
MGPIRGLRKKKKLERKLDSNGTASDSSEKDDAIDWWDDFSKRTNGLHSASKGLDRFKSIF
Sbjct: 1 MGPIRGLRKKKKLERKLDSNGTASDSSEKDDAIDWWDDFSKRTNGLHSASKGLDRFKSIF 60
Query: 61 KVSRKTFDYICLLVKDDMTAKSGHFTFLNGRPLSLCDQVAVALRRLGSGESLVTIGDSLG 120
KVSRKTFDYICLLVKDDMTAKSGHFTFLNGRPLSLCDQVAVALRRLGSGESLVTIGDSLG
Sbjct: 61 KVSRKTFDYICLLVKDDMTAKSGHFTFLNGRPLSLCDQVAVALRRLGSGESLVTIGDSLG 120
Query: 121 LNHSTVSQVTWRFVESMEERGLCHLHWPSNEVEMAQVKSKFEKIQGLPNCCGSIDTTHIT 180
LNHSTVSQVTWRFVESMEERGLCHLHWPSNEVEMAQVKSKFEKIQGLPNCCGSIDTTHIT
Sbjct: 121 LNHSTVSQVTWRFVESMEERGLCHLHWPSNEVEMAQVKSKFEKIQGLPNCCGSIDTTHIT 180
Query: 181 MCLPASDPTSFVWLDDEKNHSMVLQVIVDAEMRFRDILTGLPGKLSDRLVFQSSNFHKLC 240
MCLPASDPTSFVWLDDEKNHSMVLQVIVDAEMRFRDILTGLPGKLSDRLVFQSSNFHKLC
Sbjct: 181 MCLPASDPTSFVWLDDEKNHSMVLQVIVDAEMRFRDILTGLPGKLSDRLVFQSSNFHKLC 240
Query: 241 DKGERLNGKRLELPDRSEIQEYIVGDSGYPLLSYLVTPYDGKELSTSKAEFNKRHTATRL 300
DKGERLNGKRLELPDRSEIQEYIVGDSGYPLLSYLVTPYDGKELSTSKAEFNKRHTATRL
Sbjct: 241 DKGERLNGKRLELPDRSEIQEYIVGDSGYPLLSYLVTPYDGKELSTSKAEFNKRHTATRL 300
Query: 301 VVQQALAMLKERWRIIQGVMWRPDKHRLPRIILVCCLLHNIIIDIGDETEDGDVPLSIEH 360
VVQQALAMLKERWRIIQGVMWRPDKHRLPRIILVCCLLHNIIIDIGDETEDGDVPLSIEH
Sbjct: 301 VVQQALAMLKERWRIIQGVMWRPDKHRLPRIILVCCLLHNIIIDIGDETEDGDVPLSIEH 360
Query: 361 DVDYKQQVCDVFDSKGAYLRERLSLLFI 389
DVDYKQQVCDVFDSKGAYLRERLSLLFI
Sbjct: 361 DVDYKQQVCDVFDSKGAYLRERLSLLFI 388
BLAST of Cmc12g0317571 vs. NCBI nr
Match:
TYK01291.1 (putative nuclease HARBI1 [Cucumis melo var. makuwa])
HSP 1 Score: 790.0 bits (2039), Expect = 9.1e-225
Identity = 387/388 (99.74%), Postives = 387/388 (99.74%), Query Frame = 0
Query: 1 MGPIRGLRKKKKLERKLDSNGTASDSSEKDDAIDWWDDFSKRTNGLHSASKGLDRFKSIF 60
MGPIRGLRKKKKLERKLDSNGTASDSSEKDDAIDWWDDFSKRTNGLHSASKGLDRFKSIF
Sbjct: 1 MGPIRGLRKKKKLERKLDSNGTASDSSEKDDAIDWWDDFSKRTNGLHSASKGLDRFKSIF 60
Query: 61 KVSRKTFDYICLLVKDDMTAKSGHFTFLNGRPLSLCDQVAVALRRLGSGESLVTIGDSLG 120
KVSRKTFDYICLLVKDDMTAKSGHFTFLNGRPLSLCDQVAVALRRLGSGESLVTIGDSLG
Sbjct: 61 KVSRKTFDYICLLVKDDMTAKSGHFTFLNGRPLSLCDQVAVALRRLGSGESLVTIGDSLG 120
Query: 121 LNHSTVSQVTWRFVESMEERGLCHLHWPSNEVEMAQVKSKFEKIQGLPNCCGSIDTTHIT 180
LNHSTVSQVTWRFVESMEERGL HLHWPSNEVEMAQVKSKFEKIQGLPNCCGSIDTTHIT
Sbjct: 121 LNHSTVSQVTWRFVESMEERGLRHLHWPSNEVEMAQVKSKFEKIQGLPNCCGSIDTTHIT 180
Query: 181 MCLPASDPTSFVWLDDEKNHSMVLQVIVDAEMRFRDILTGLPGKLSDRLVFQSSNFHKLC 240
MCLPASDPTSFVWLDDEKNHSMVLQVIVDAEMRFRDILTGLPGKLSDRLVFQSSNFHKLC
Sbjct: 181 MCLPASDPTSFVWLDDEKNHSMVLQVIVDAEMRFRDILTGLPGKLSDRLVFQSSNFHKLC 240
Query: 241 DKGERLNGKRLELPDRSEIQEYIVGDSGYPLLSYLVTPYDGKELSTSKAEFNKRHTATRL 300
DKGERLNGKRLELPDRSEIQEYIVGDSGYPLLSYLVTPYDGKELSTSKAEFNKRHTATRL
Sbjct: 241 DKGERLNGKRLELPDRSEIQEYIVGDSGYPLLSYLVTPYDGKELSTSKAEFNKRHTATRL 300
Query: 301 VVQQALAMLKERWRIIQGVMWRPDKHRLPRIILVCCLLHNIIIDIGDETEDGDVPLSIEH 360
VVQQALAMLKERWRIIQGVMWRPDKHRLPRIILVCCLLHNIIIDIGDETEDGDVPLSIEH
Sbjct: 301 VVQQALAMLKERWRIIQGVMWRPDKHRLPRIILVCCLLHNIIIDIGDETEDGDVPLSIEH 360
Query: 361 DVDYKQQVCDVFDSKGAYLRERLSLLFI 389
DVDYKQQVCDVFDSKGAYLRERLSLLFI
Sbjct: 361 DVDYKQQVCDVFDSKGAYLRERLSLLFI 388
BLAST of Cmc12g0317571 vs. NCBI nr
Match:
XP_008457540.1 (PREDICTED: LOW QUALITY PROTEIN: putative nuclease HARBI1 [Cucumis melo])
HSP 1 Score: 790.0 bits (2039), Expect = 9.1e-225
Identity = 386/388 (99.48%), Postives = 386/388 (99.48%), Query Frame = 0
Query: 1 MGPIRGLRKKKKLERKLDSNGTASDSSEKDDAIDWWDDFSKRTNGLHSASKGLDRFKSIF 60
MGPIRGLRKKKKLERKLDSNGTASDSSEKDDAIDWWDDFSKRTNGLHSASKGLDRFKSIF
Sbjct: 1 MGPIRGLRKKKKLERKLDSNGTASDSSEKDDAIDWWDDFSKRTNGLHSASKGLDRFKSIF 60
Query: 61 KVSRKTFDYICLLVKDDMTAKSGHFTFLNGRPLSLCDQVAVALRRLGSGESLVTIGDSLG 120
KVSRKTFDYICLLVKDDMTAKSGHFTFLNGRPLSLCDQVAVALRRLGSGESLVTIGDSLG
Sbjct: 61 KVSRKTFDYICLLVKDDMTAKSGHFTFLNGRPLSLCDQVAVALRRLGSGESLVTIGDSLG 120
Query: 121 LNHSTVSQVTWRFVESMEERGLCHLHWPSNEVEMAQVKSKFEKIQGLPNCCGSIDTTHIT 180
LNHSTVSQVTWRFVESMEERGLCHLHWPSNEVEMAQVKSKFEKIQGLPNCCGSIDTTHIT
Sbjct: 121 LNHSTVSQVTWRFVESMEERGLCHLHWPSNEVEMAQVKSKFEKIQGLPNCCGSIDTTHIT 180
Query: 181 MCLPASDPTSFVWLDDEKNHSMVLQVIVDAEMRFRDILTGLPGKLSDRLVFQSSNFHKLC 240
MCLPASDPTSFVWLD KNHSMVLQVIVDAEMRFRDILTGLPGKLSDRLVFQSSNFHKLC
Sbjct: 181 MCLPASDPTSFVWLDXRKNHSMVLQVIVDAEMRFRDILTGLPGKLSDRLVFQSSNFHKLC 240
Query: 241 DKGERLNGKRLELPDRSEIQEYIVGDSGYPLLSYLVTPYDGKELSTSKAEFNKRHTATRL 300
DKGERLNGKRLELPDRSEIQEYIVGDSGYPLLSYLVTPYDGKELSTSKAEFNKRHTATRL
Sbjct: 241 DKGERLNGKRLELPDRSEIQEYIVGDSGYPLLSYLVTPYDGKELSTSKAEFNKRHTATRL 300
Query: 301 VVQQALAMLKERWRIIQGVMWRPDKHRLPRIILVCCLLHNIIIDIGDETEDGDVPLSIEH 360
VVQQALAMLKERWRIIQGVMWRPDKHRLPRIILVCCLLHNIIIDIGDETEDGDVPLSIEH
Sbjct: 301 VVQQALAMLKERWRIIQGVMWRPDKHRLPRIILVCCLLHNIIIDIGDETEDGDVPLSIEH 360
Query: 361 DVDYKQQVCDVFDSKGAYLRERLSLLFI 389
DVDYKQQVCDVFDSKGAYLRERLSLLFI
Sbjct: 361 DVDYKQQVCDVFDSKGAYLRERLSLLFI 388
BLAST of Cmc12g0317571 vs. NCBI nr
Match:
XP_004149039.1 (protein ALP1-like [Cucumis sativus] >KGN65671.1 hypothetical protein Csa_019843 [Cucumis sativus])
HSP 1 Score: 764.2 bits (1972), Expect = 5.3e-217
Identity = 371/388 (95.62%), Postives = 380/388 (97.94%), Query Frame = 0
Query: 1 MGPIRGLRKKKKLERKLDSNGTASDSSEKDDAIDWWDDFSKRTNGLHSASKGLDRFKSIF 60
MGPIRGLRKKKKLERKLD NGTASDSSEKDDAIDWWDDFSKRTNGLHSASKGLDRFKSIF
Sbjct: 1 MGPIRGLRKKKKLERKLDCNGTASDSSEKDDAIDWWDDFSKRTNGLHSASKGLDRFKSIF 60
Query: 61 KVSRKTFDYICLLVKDDMTAKSGHFTFLNGRPLSLCDQVAVALRRLGSGESLVTIGDSLG 120
KVSRKTFDYICLLVKDDMTAKSGHFTFLNGRPLSLCDQVAVALRRLGSGESLVTIGDSLG
Sbjct: 61 KVSRKTFDYICLLVKDDMTAKSGHFTFLNGRPLSLCDQVAVALRRLGSGESLVTIGDSLG 120
Query: 121 LNHSTVSQVTWRFVESMEERGLCHLHWPSNEVEMAQVKSKFEKIQGLPNCCGSIDTTHIT 180
LNHSTVSQVTWRFVESMEERGL HLHWPSNEVEMAQVKSKFEKIQGLPNCCGSIDTTHIT
Sbjct: 121 LNHSTVSQVTWRFVESMEERGLHHLHWPSNEVEMAQVKSKFEKIQGLPNCCGSIDTTHIT 180
Query: 181 MCLPASDPTSFVWLDDEKNHSMVLQVIVDAEMRFRDILTGLPGKLSDRLVFQSSNFHKLC 240
MCLPASDPTS+VWLDD+KNHSMVLQVIVDAEMRFRDILTGLPGKLSD LVFQSSNFHKLC
Sbjct: 181 MCLPASDPTSYVWLDDKKNHSMVLQVIVDAEMRFRDILTGLPGKLSDWLVFQSSNFHKLC 240
Query: 241 DKGERLNGKRLELPDRSEIQEYIVGDSGYPLLSYLVTPYDGKELSTSKAEFNKRHTATRL 300
DKGERLNGKR ELPDRSEI+EYI+GDSGYPLL YLVTPYDGKELSTSK EFNKRH TRL
Sbjct: 241 DKGERLNGKRFELPDRSEIREYIIGDSGYPLLPYLVTPYDGKELSTSKTEFNKRHKETRL 300
Query: 301 VVQQALAMLKERWRIIQGVMWRPDKHRLPRIILVCCLLHNIIIDIGDETEDGDVPLSIEH 360
VVQ+ALAMLKERWRIIQGVMWRPDKHRLPRIILVCCLLHNIIIDIGDETE+G+VPLSIEH
Sbjct: 301 VVQRALAMLKERWRIIQGVMWRPDKHRLPRIILVCCLLHNIIIDIGDETEEGEVPLSIEH 360
Query: 361 DVDYKQQVCDVFDSKGAYLRERLSLLFI 389
DVDYKQQVCDVFDSKGAY+R+RLSLLFI
Sbjct: 361 DVDYKQQVCDVFDSKGAYVRDRLSLLFI 388
BLAST of Cmc12g0317571 vs. NCBI nr
Match:
XP_038893506.1 (protein ALP1-like [Benincasa hispida])
HSP 1 Score: 751.5 bits (1939), Expect = 3.6e-213
Identity = 369/389 (94.86%), Postives = 378/389 (97.17%), Query Frame = 0
Query: 1 MGPIRGLRKKKKLERKLDSNGTASDSSEKDDAIDWWDDFSKRTNGLHSASKGLDRFKSIF 60
MGPIRGLRKKKKLERKLDSNGTASDSSEKD+AIDWWDDFSKRTNGLHSASKGLDRFKSIF
Sbjct: 1 MGPIRGLRKKKKLERKLDSNGTASDSSEKDEAIDWWDDFSKRTNGLHSASKGLDRFKSIF 60
Query: 61 KVSRKTFDYICLLVKDDMTAKSGHFTFLNGRPLSLCDQVAVALRRLGSGESLVTIGDSLG 120
KVSRKTFDYICLLVKDDMTAKSGHFTFLNGRPLSLCDQVAVALRRLGSGESLVTIGDSLG
Sbjct: 61 KVSRKTFDYICLLVKDDMTAKSGHFTFLNGRPLSLCDQVAVALRRLGSGESLVTIGDSLG 120
Query: 121 LNHSTVSQVTWRFVESMEERGLCHLHWPSNEVEMAQVKSKFEKIQGLPNCCGSIDTTHIT 180
LNHSTVSQVTWRFVESMEERGL HLHWPSNEVEMAQVKSKFEKIQGLPNCCGSIDTTHIT
Sbjct: 121 LNHSTVSQVTWRFVESMEERGLRHLHWPSNEVEMAQVKSKFEKIQGLPNCCGSIDTTHIT 180
Query: 181 MCLPASDPTSFVWLDDEKNHSMVLQVIVDAEMRFRDILTGLPGKLSDRLVFQSSNFHKLC 240
MCLPASD TS+VWLD+EKNHSMVLQVIVDAEMRFRDILTGLPGK+SD LVFQSSNFHKLC
Sbjct: 181 MCLPASDSTSYVWLDEEKNHSMVLQVIVDAEMRFRDILTGLPGKMSDWLVFQSSNFHKLC 240
Query: 241 DKGERLNGKRLELPDRSEIQEYIVGDSGYPLLSYLVTPYDGKELSTSKAEFNKRHTATRL 300
DKGERLNGKRLEL DRSEI+EYI+GDSGYPLL YLVTPYDGKE STSKAEFNKRH TRL
Sbjct: 241 DKGERLNGKRLELNDRSEIREYIIGDSGYPLLPYLVTPYDGKEHSTSKAEFNKRHKETRL 300
Query: 301 VVQQALAMLKERWRIIQGVMWRPDKHRLPRIILVCCLLHNIIIDIGDET-EDGDVPLSIE 360
VVQ+ALAMLKERWRIIQGVMWRPDKHRLPRIILVCCLLHNIIIDIGD+T EDG VPLSIE
Sbjct: 301 VVQRALAMLKERWRIIQGVMWRPDKHRLPRIILVCCLLHNIIIDIGDDTEEDGGVPLSIE 360
Query: 361 HDVDYKQQVCDVFDSKGAYLRERLSLLFI 389
HDVDYKQQVCDVFD KGAYLR+RLSLLFI
Sbjct: 361 HDVDYKQQVCDVFDPKGAYLRDRLSLLFI 389
BLAST of Cmc12g0317571 vs. ExPASy Swiss-Prot
Match:
Q9M2U3 (Protein ALP1-like OS=Arabidopsis thaliana OX=3702 GN=At3g55350 PE=2 SV=1)
HSP 1 Score: 454.9 bits (1169), Expect = 9.1e-127
Identity = 224/403 (55.58%), Postives = 294/403 (72.95%), Query Frame = 0
Query: 1 MGPIRGLRKKKKLERKLDSN--------------GTASDSSEKDD-----AIDWWDDFSK 60
MGPI+ ++KKK+ E+K+D N A ++++ DD ++DWWD FS+
Sbjct: 1 MGPIKTIKKKKRAEKKVDRNVLLAATAAATSASAAAALNNNDDDDDSSSQSLDWWDGFSR 60
Query: 61 RTNGLHSASKGLDRFKSIFKVSRKTFDYICLLVKDDMTAKSGHFTFLNGRPLSLCDQVAV 120
R ++ S F+S+FK+SRKTFDYIC LVK D TAK +F+ NG PLSL D+VAV
Sbjct: 61 R---IYGGSTDPKTFESVFKISRKTFDYICSLVKADFTAKPANFSDSNGNPLSLNDRVAV 120
Query: 121 ALRRLGSGESLVTIGDSLGLNHSTVSQVTWRFVESMEERGLCHLHWPSNEVEMAQVKSKF 180
ALRRLGSGESL IG++ G+N STVSQ+TWRFVESMEER + HL WPS ++ ++KSKF
Sbjct: 121 ALRRLGSGESLSVIGETFGMNQSTVSQITWRFVESMEERAIHHLSWPS---KLDEIKSKF 180
Query: 181 EKIQGLPNCCGSIDTTHITMCLPASDPTSFVWLDDEKNHSMVLQVIVDAEMRFRDILTGL 240
EKI GLPNCCG+ID THI M LPA +P++ VWLD EKN SM LQ +VD +MRF D++ G
Sbjct: 181 EKISGLPNCCGAIDITHIVMNLPAVEPSNKVWLDGEKNFSMTLQAVVDPDMRFLDVIAGW 240
Query: 241 PGKLSDRLVFQSSNFHKLCDKGERLNGKRLELPDRSEIQEYIVGDSGYPLLSYLVTPYDG 300
PG L+D +V ++S F+KL +KG+RLNG++L L +R+E++EYIVGDSG+PLL +L+TPY G
Sbjct: 241 PGSLNDDVVLKNSGFYKLVEKGKRLNGEKLPLSERTELREYIVGDSGFPLLPWLLTPYQG 300
Query: 301 KELSTSKAEFNKRHTATRLVVQQALAMLKERWRIIQGVMWRPDKHRLPRIILVCCLLHNI 360
K S + EFNKRH+ Q AL+ LK+RWRII GVMW PD++RLPRII VCCLLHNI
Sbjct: 301 KPTSLPQTEFNKRHSEATKAAQMALSKLKDRWRIINGVMWMPDRNRLPRIIFVCCLLHNI 360
Query: 361 IIDIGDETEDGDVPLSIEHDVDYKQQVCDVFDSKGAYLRERLS 385
IID+ D+T D D PLS +HD++Y+Q+ C + D + LR+ LS
Sbjct: 361 IIDMEDQTLD-DQPLSQQHDMNYRQRSCKLADEASSVLRDELS 396
BLAST of Cmc12g0317571 vs. ExPASy Swiss-Prot
Match:
Q94K49 (Protein ANTAGONIST OF LIKE HETEROCHROMATIN PROTEIN 1 OS=Arabidopsis thaliana OX=3702 GN=ALP1 PE=1 SV=1)
HSP 1 Score: 329.3 bits (843), Expect = 5.7e-89
Identity = 180/393 (45.80%), Postives = 243/393 (61.83%), Query Frame = 0
Query: 1 MGPIRGLRKKKKLERKLDSNGTASDSSEK---------DDAI--DWWDDFSKRTNGLHSA 60
M P++ +KKK ++ LD + + EK +AI DWWD F R +
Sbjct: 1 MAPVK--QKKKNKKKPLDKAKKLAKNKEKKRVNAVPLDPEAIDCDWWDTFWLRNSSPSVP 60
Query: 61 SKGLDRFKSIFKVSRKTFDYICLLVKDDMTAK--SGHFTFLNGRPLSLCDQVAVALRRLG 120
S FK F+ S+ TF YIC LV++D+ ++ SG + GR LS+ QVA+ALRRL
Sbjct: 61 SDEDYAFKHFFRASKTTFSYICSLVREDLISRPPSGLIN-IEGRLLSVEKQVAIALRRLA 120
Query: 121 SGESLVTIGDSLGLNHSTVSQVTWRFVESMEERGLCHLHWPSNEVEMAQVKSKFEKIQGL 180
SG+S V++G + G+ STVSQVTWRF+E++EER HL WP ++ + ++KSKFE++ GL
Sbjct: 121 SGDSQVSVGAAFGVGQSTVSQVTWRFIEALEERAKHHLRWPDSD-RIEEIKSKFEEMYGL 180
Query: 181 PNCCGSIDTTHITMCLPASDPTSFVWLDDEKNHSMVLQVIVDAEMRFRDILTGLPGKLSD 240
PNCCG+IDTTHI M LPA S W D EKN+SM LQ + D EMRF +++TG PG ++
Sbjct: 181 PNCCGAIDTTHIIMTLPAVQ-ASDDWCDQEKNYSMFLQGVFDHEMRFLNMVTGWPGGMTV 240
Query: 241 RLVFQSSNFHKLCDKGERLNGKRLELPDRSEIQEYIVGDSGYPLLSYLVTPYDGKELSTS 300
+ + S F KLC+ + L+G L ++I+EY+VG YPLL +L+TP+D S S
Sbjct: 241 SKLLKFSGFFKLCENAQILDGNPKTLSQGAQIREYVVGGISYPLLPWLITPHDSDHPSDS 300
Query: 301 KAEFNKRHTATRLVVQQALAMLKERWRIIQGVMWRPDKHRLPRIILVCCLLHNIIIDIGD 360
FN+RH R V A LK WRI+ VMWRPD+ +LP IILVCCLLHNIIID GD
Sbjct: 301 MVAFNERHEKVRSVAATAFQQLKGSWRILSKVMWRPDRRKLPSIILVCCLLHNIIIDCGD 360
Query: 361 ETEDGDVPLSIEHDVDYKQQVCDVFDSKGAYLR 381
++ DVPLS HD Y + C + G+ LR
Sbjct: 361 YLQE-DVPLSGHHDSGYADRYCKQTEPLGSELR 387
BLAST of Cmc12g0317571 vs. ExPASy Swiss-Prot
Match:
Q6AZB8 (Putative nuclease HARBI1 OS=Danio rerio OX=7955 GN=harbi1 PE=2 SV=1)
HSP 1 Score: 125.2 bits (313), Expect = 1.6e-27
Identity = 86/310 (27.74%), Postives = 150/310 (48.39%), Query Frame = 0
Query: 51 KGLDRFK----------SIFKVSRKTFDYICLLVKDDMTAKSGHFTFLNGRPLSLCDQVA 110
K LDRF + F R+ Y+ L+KD + ++ R +S Q+
Sbjct: 20 KTLDRFDIETVSDDFLLNTFGFPREFIYYLVELLKDSLLRRT-----QRSRAISPDVQIL 79
Query: 111 VALRRLGSGESLVTIGDSLGLNHSTVSQVTWRFVESMEERGLCHLHWPSNEVEMAQVKSK 170
AL SG +GD++G++ +++S+ +++ E+ + + +E Q K +
Sbjct: 80 AALGFYTSGSFQSKMGDAIGISQASMSRCVSNVTKALIEKAPEFIGFTRDEATKQQFKDE 139
Query: 171 FEKIQGLPNCCGSIDTTHITMCLPASDPTSFVWLDDEKNHSMVLQVIVDAEMRFRDILTG 230
F +I G+PN G +D HI + P +D +S+V + + HS+ Q++ DA T
Sbjct: 140 FYRIAGIPNVTGVVDCAHIAIKAPNADDSSYV--NKKGFHSINCQLVCDARGLLLSAETH 199
Query: 231 LPGKLSDRLVFQSSNFHKLCDKGERLNGKRLELPDRSEIQEYIVGDSGYPLLSYLVTPYD 290
PG L+DR VF+ SN KL ++ E ++ + +++GD+ YPL +L+TP
Sbjct: 200 WPGSLTDRAVFKQSNVAKLFEEQE------------NDDEGWLLGDNRYPLKKWLMTPVQ 259
Query: 291 GKELSTSKAEFNKRHTATRLVVQQALAMLKERWRIIQG----VMWRPDKHRLPRIILVCC 347
E S + +N HT T +V + ++ R+R + G + + P+K II CC
Sbjct: 260 SPE-SPADYRYNLAHTTTHEIVDRTFRAIQTRFRCLDGAKGYLQYSPEK--CSHIIQACC 307
BLAST of Cmc12g0317571 vs. ExPASy Swiss-Prot
Match:
Q8BR93 (Putative nuclease HARBI1 OS=Mus musculus OX=10090 GN=Harbi1 PE=2 SV=1)
HSP 1 Score: 121.3 bits (303), Expect = 2.4e-26
Identity = 81/299 (27.09%), Postives = 146/299 (48.83%), Query Frame = 0
Query: 91 RPLSLCDQVAVALRRLGSGESLVTIGDSLGLNHSTVSQVTWRFVESMEERGLCHLHWPSN 150
R +S Q+ AL SG +GD++G++ +++S+ E++ ER +H+P +
Sbjct: 65 RAISPETQILAALGFYTSGSFQTRMGDAIGISQASMSRCVANVTEALVERASQFIHFPVD 124
Query: 151 EVEMAQVKSKFEKIQGLPNCCGSIDTTHITMCLPASDPTSFVWLDDEKNHSMVLQVIVDA 210
E + +K +F + G+P G D H+ + P ++ S+V + + HS+ V+ D
Sbjct: 125 EAAVQSLKDEFYGLAGMPGVIGVADCIHVAIKAPNAEDLSYV--NRKGLHSLNCLVVCDI 184
Query: 211 EMRFRDILTGLPGKLSDRLVFQSSNFHKLCDKGERLNGKRLELPDRSEIQEYIVGDSGYP 270
+ T PG L D V Q S+ + G +P S +++GDS +
Sbjct: 185 RGALMTVETSWPGSLQDCAVLQRSSLTSQFETG---------MPKDS----WLLGDSSFF 244
Query: 271 LLSYLVTPYDGKELSTSKAEFNKRHTATRLVVQQALAMLKERWRIIQG----VMWRPDKH 330
L S+L+TP E + ++ +N+ H+AT V+++ L L R+R + G + + P+K
Sbjct: 245 LRSWLLTPLPIPE-TAAEYRYNRAHSATHSVIERTLQTLCCRFRCLDGSKGALQYSPEK- 304
Query: 331 RLPRIILVCCLLHNIIIDIGDETEDGDVPLSIEHDVDYKQQVCDVFDSKGAYLRERLSL 386
IIL CC+LHNI +D G + VP I+ + + + + D + +R+ L L
Sbjct: 305 -CSHIILACCVLHNISLDHGMDVWSSPVPGPIDQPPEGEDEHMESLDLEADRIRQELIL 345
BLAST of Cmc12g0317571 vs. ExPASy Swiss-Prot
Match:
B0BN95 (Putative nuclease HARBI1 OS=Rattus norvegicus OX=10116 GN=Harbi1 PE=2 SV=1)
HSP 1 Score: 121.3 bits (303), Expect = 2.4e-26
Identity = 79/299 (26.42%), Postives = 148/299 (49.50%), Query Frame = 0
Query: 91 RPLSLCDQVAVALRRLGSGESLVTIGDSLGLNHSTVSQVTWRFVESMEERGLCHLHWPSN 150
R +S Q+ AL SG +GD++G++ +++S+ E++ ER +H+P++
Sbjct: 65 RAISPETQILAALGFYTSGSFQTRMGDAIGISQASMSRCVANVTEALVERASQFIHFPAD 124
Query: 151 EVEMAQVKSKFEKIQGLPNCCGSIDTTHITMCLPASDPTSFVWLDDEKNHSMVLQVIVDA 210
E + +K +F + G+P G++D H+ + P ++ S+V + + HS+ V+ D
Sbjct: 125 EAAIQSLKDEFYGLAGMPGVIGAVDCIHVAIKAPNAEDLSYV--NRKGLHSLNCLVVCDI 184
Query: 211 EMRFRDILTGLPGKLSDRLVFQSSNFHKLCDKGERLNGKRLELPDRSEIQEYIVGDSGYP 270
+ T PG L D V Q S+ + G +P S +++GDS +
Sbjct: 185 RGALMTVETSWPGSLQDCAVLQQSSLSSQFETG---------MPKDS----WLLGDSSFF 244
Query: 271 LLSYLVTPYDGKELSTSKAEFNKRHTATRLVVQQALAMLKERWRIIQG----VMWRPDKH 330
L ++L+TP E + ++ +N+ H+AT V+++ L L R+R + G + + P+K
Sbjct: 245 LHTWLLTPLHIPE-TPAEYRYNRAHSATHSVIEKTLRTLCCRFRCLDGSKGALQYSPEKS 304
Query: 331 RLPRIILVCCLLHNIIIDIGDETEDGDVPLSIEHDVDYKQQVCDVFDSKGAYLRERLSL 386
IIL CC+LHNI ++ G + V IE + + + + D + +R+ L L
Sbjct: 305 --SHIILACCVLHNISLEHGMDVWSSPVTGPIEQPPEGEDEQMESLDLEADRIRQELIL 345
BLAST of Cmc12g0317571 vs. ExPASy TrEMBL
Match:
A0A5A7SXL0 (Putative nuclease HARBI1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold43059G001000 PE=3 SV=1)
HSP 1 Score: 794.7 bits (2051), Expect = 1.8e-226
Identity = 388/388 (100.00%), Postives = 388/388 (100.00%), Query Frame = 0
Query: 1 MGPIRGLRKKKKLERKLDSNGTASDSSEKDDAIDWWDDFSKRTNGLHSASKGLDRFKSIF 60
MGPIRGLRKKKKLERKLDSNGTASDSSEKDDAIDWWDDFSKRTNGLHSASKGLDRFKSIF
Sbjct: 1 MGPIRGLRKKKKLERKLDSNGTASDSSEKDDAIDWWDDFSKRTNGLHSASKGLDRFKSIF 60
Query: 61 KVSRKTFDYICLLVKDDMTAKSGHFTFLNGRPLSLCDQVAVALRRLGSGESLVTIGDSLG 120
KVSRKTFDYICLLVKDDMTAKSGHFTFLNGRPLSLCDQVAVALRRLGSGESLVTIGDSLG
Sbjct: 61 KVSRKTFDYICLLVKDDMTAKSGHFTFLNGRPLSLCDQVAVALRRLGSGESLVTIGDSLG 120
Query: 121 LNHSTVSQVTWRFVESMEERGLCHLHWPSNEVEMAQVKSKFEKIQGLPNCCGSIDTTHIT 180
LNHSTVSQVTWRFVESMEERGLCHLHWPSNEVEMAQVKSKFEKIQGLPNCCGSIDTTHIT
Sbjct: 121 LNHSTVSQVTWRFVESMEERGLCHLHWPSNEVEMAQVKSKFEKIQGLPNCCGSIDTTHIT 180
Query: 181 MCLPASDPTSFVWLDDEKNHSMVLQVIVDAEMRFRDILTGLPGKLSDRLVFQSSNFHKLC 240
MCLPASDPTSFVWLDDEKNHSMVLQVIVDAEMRFRDILTGLPGKLSDRLVFQSSNFHKLC
Sbjct: 181 MCLPASDPTSFVWLDDEKNHSMVLQVIVDAEMRFRDILTGLPGKLSDRLVFQSSNFHKLC 240
Query: 241 DKGERLNGKRLELPDRSEIQEYIVGDSGYPLLSYLVTPYDGKELSTSKAEFNKRHTATRL 300
DKGERLNGKRLELPDRSEIQEYIVGDSGYPLLSYLVTPYDGKELSTSKAEFNKRHTATRL
Sbjct: 241 DKGERLNGKRLELPDRSEIQEYIVGDSGYPLLSYLVTPYDGKELSTSKAEFNKRHTATRL 300
Query: 301 VVQQALAMLKERWRIIQGVMWRPDKHRLPRIILVCCLLHNIIIDIGDETEDGDVPLSIEH 360
VVQQALAMLKERWRIIQGVMWRPDKHRLPRIILVCCLLHNIIIDIGDETEDGDVPLSIEH
Sbjct: 301 VVQQALAMLKERWRIIQGVMWRPDKHRLPRIILVCCLLHNIIIDIGDETEDGDVPLSIEH 360
Query: 361 DVDYKQQVCDVFDSKGAYLRERLSLLFI 389
DVDYKQQVCDVFDSKGAYLRERLSLLFI
Sbjct: 361 DVDYKQQVCDVFDSKGAYLRERLSLLFI 388
BLAST of Cmc12g0317571 vs. ExPASy TrEMBL
Match:
A0A5D3BNB9 (Putative nuclease HARBI1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold49G00830 PE=3 SV=1)
HSP 1 Score: 790.0 bits (2039), Expect = 4.4e-225
Identity = 387/388 (99.74%), Postives = 387/388 (99.74%), Query Frame = 0
Query: 1 MGPIRGLRKKKKLERKLDSNGTASDSSEKDDAIDWWDDFSKRTNGLHSASKGLDRFKSIF 60
MGPIRGLRKKKKLERKLDSNGTASDSSEKDDAIDWWDDFSKRTNGLHSASKGLDRFKSIF
Sbjct: 1 MGPIRGLRKKKKLERKLDSNGTASDSSEKDDAIDWWDDFSKRTNGLHSASKGLDRFKSIF 60
Query: 61 KVSRKTFDYICLLVKDDMTAKSGHFTFLNGRPLSLCDQVAVALRRLGSGESLVTIGDSLG 120
KVSRKTFDYICLLVKDDMTAKSGHFTFLNGRPLSLCDQVAVALRRLGSGESLVTIGDSLG
Sbjct: 61 KVSRKTFDYICLLVKDDMTAKSGHFTFLNGRPLSLCDQVAVALRRLGSGESLVTIGDSLG 120
Query: 121 LNHSTVSQVTWRFVESMEERGLCHLHWPSNEVEMAQVKSKFEKIQGLPNCCGSIDTTHIT 180
LNHSTVSQVTWRFVESMEERGL HLHWPSNEVEMAQVKSKFEKIQGLPNCCGSIDTTHIT
Sbjct: 121 LNHSTVSQVTWRFVESMEERGLRHLHWPSNEVEMAQVKSKFEKIQGLPNCCGSIDTTHIT 180
Query: 181 MCLPASDPTSFVWLDDEKNHSMVLQVIVDAEMRFRDILTGLPGKLSDRLVFQSSNFHKLC 240
MCLPASDPTSFVWLDDEKNHSMVLQVIVDAEMRFRDILTGLPGKLSDRLVFQSSNFHKLC
Sbjct: 181 MCLPASDPTSFVWLDDEKNHSMVLQVIVDAEMRFRDILTGLPGKLSDRLVFQSSNFHKLC 240
Query: 241 DKGERLNGKRLELPDRSEIQEYIVGDSGYPLLSYLVTPYDGKELSTSKAEFNKRHTATRL 300
DKGERLNGKRLELPDRSEIQEYIVGDSGYPLLSYLVTPYDGKELSTSKAEFNKRHTATRL
Sbjct: 241 DKGERLNGKRLELPDRSEIQEYIVGDSGYPLLSYLVTPYDGKELSTSKAEFNKRHTATRL 300
Query: 301 VVQQALAMLKERWRIIQGVMWRPDKHRLPRIILVCCLLHNIIIDIGDETEDGDVPLSIEH 360
VVQQALAMLKERWRIIQGVMWRPDKHRLPRIILVCCLLHNIIIDIGDETEDGDVPLSIEH
Sbjct: 301 VVQQALAMLKERWRIIQGVMWRPDKHRLPRIILVCCLLHNIIIDIGDETEDGDVPLSIEH 360
Query: 361 DVDYKQQVCDVFDSKGAYLRERLSLLFI 389
DVDYKQQVCDVFDSKGAYLRERLSLLFI
Sbjct: 361 DVDYKQQVCDVFDSKGAYLRERLSLLFI 388
BLAST of Cmc12g0317571 vs. ExPASy TrEMBL
Match:
A0A1S3C6F2 (LOW QUALITY PROTEIN: putative nuclease HARBI1 OS=Cucumis melo OX=3656 GN=LOC103497206 PE=3 SV=1)
HSP 1 Score: 790.0 bits (2039), Expect = 4.4e-225
Identity = 386/388 (99.48%), Postives = 386/388 (99.48%), Query Frame = 0
Query: 1 MGPIRGLRKKKKLERKLDSNGTASDSSEKDDAIDWWDDFSKRTNGLHSASKGLDRFKSIF 60
MGPIRGLRKKKKLERKLDSNGTASDSSEKDDAIDWWDDFSKRTNGLHSASKGLDRFKSIF
Sbjct: 1 MGPIRGLRKKKKLERKLDSNGTASDSSEKDDAIDWWDDFSKRTNGLHSASKGLDRFKSIF 60
Query: 61 KVSRKTFDYICLLVKDDMTAKSGHFTFLNGRPLSLCDQVAVALRRLGSGESLVTIGDSLG 120
KVSRKTFDYICLLVKDDMTAKSGHFTFLNGRPLSLCDQVAVALRRLGSGESLVTIGDSLG
Sbjct: 61 KVSRKTFDYICLLVKDDMTAKSGHFTFLNGRPLSLCDQVAVALRRLGSGESLVTIGDSLG 120
Query: 121 LNHSTVSQVTWRFVESMEERGLCHLHWPSNEVEMAQVKSKFEKIQGLPNCCGSIDTTHIT 180
LNHSTVSQVTWRFVESMEERGLCHLHWPSNEVEMAQVKSKFEKIQGLPNCCGSIDTTHIT
Sbjct: 121 LNHSTVSQVTWRFVESMEERGLCHLHWPSNEVEMAQVKSKFEKIQGLPNCCGSIDTTHIT 180
Query: 181 MCLPASDPTSFVWLDDEKNHSMVLQVIVDAEMRFRDILTGLPGKLSDRLVFQSSNFHKLC 240
MCLPASDPTSFVWLD KNHSMVLQVIVDAEMRFRDILTGLPGKLSDRLVFQSSNFHKLC
Sbjct: 181 MCLPASDPTSFVWLDXRKNHSMVLQVIVDAEMRFRDILTGLPGKLSDRLVFQSSNFHKLC 240
Query: 241 DKGERLNGKRLELPDRSEIQEYIVGDSGYPLLSYLVTPYDGKELSTSKAEFNKRHTATRL 300
DKGERLNGKRLELPDRSEIQEYIVGDSGYPLLSYLVTPYDGKELSTSKAEFNKRHTATRL
Sbjct: 241 DKGERLNGKRLELPDRSEIQEYIVGDSGYPLLSYLVTPYDGKELSTSKAEFNKRHTATRL 300
Query: 301 VVQQALAMLKERWRIIQGVMWRPDKHRLPRIILVCCLLHNIIIDIGDETEDGDVPLSIEH 360
VVQQALAMLKERWRIIQGVMWRPDKHRLPRIILVCCLLHNIIIDIGDETEDGDVPLSIEH
Sbjct: 301 VVQQALAMLKERWRIIQGVMWRPDKHRLPRIILVCCLLHNIIIDIGDETEDGDVPLSIEH 360
Query: 361 DVDYKQQVCDVFDSKGAYLRERLSLLFI 389
DVDYKQQVCDVFDSKGAYLRERLSLLFI
Sbjct: 361 DVDYKQQVCDVFDSKGAYLRERLSLLFI 388
BLAST of Cmc12g0317571 vs. ExPASy TrEMBL
Match:
A0A0A0M0C2 (DDE Tnp4 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G481730 PE=3 SV=1)
HSP 1 Score: 764.2 bits (1972), Expect = 2.6e-217
Identity = 371/388 (95.62%), Postives = 380/388 (97.94%), Query Frame = 0
Query: 1 MGPIRGLRKKKKLERKLDSNGTASDSSEKDDAIDWWDDFSKRTNGLHSASKGLDRFKSIF 60
MGPIRGLRKKKKLERKLD NGTASDSSEKDDAIDWWDDFSKRTNGLHSASKGLDRFKSIF
Sbjct: 1 MGPIRGLRKKKKLERKLDCNGTASDSSEKDDAIDWWDDFSKRTNGLHSASKGLDRFKSIF 60
Query: 61 KVSRKTFDYICLLVKDDMTAKSGHFTFLNGRPLSLCDQVAVALRRLGSGESLVTIGDSLG 120
KVSRKTFDYICLLVKDDMTAKSGHFTFLNGRPLSLCDQVAVALRRLGSGESLVTIGDSLG
Sbjct: 61 KVSRKTFDYICLLVKDDMTAKSGHFTFLNGRPLSLCDQVAVALRRLGSGESLVTIGDSLG 120
Query: 121 LNHSTVSQVTWRFVESMEERGLCHLHWPSNEVEMAQVKSKFEKIQGLPNCCGSIDTTHIT 180
LNHSTVSQVTWRFVESMEERGL HLHWPSNEVEMAQVKSKFEKIQGLPNCCGSIDTTHIT
Sbjct: 121 LNHSTVSQVTWRFVESMEERGLHHLHWPSNEVEMAQVKSKFEKIQGLPNCCGSIDTTHIT 180
Query: 181 MCLPASDPTSFVWLDDEKNHSMVLQVIVDAEMRFRDILTGLPGKLSDRLVFQSSNFHKLC 240
MCLPASDPTS+VWLDD+KNHSMVLQVIVDAEMRFRDILTGLPGKLSD LVFQSSNFHKLC
Sbjct: 181 MCLPASDPTSYVWLDDKKNHSMVLQVIVDAEMRFRDILTGLPGKLSDWLVFQSSNFHKLC 240
Query: 241 DKGERLNGKRLELPDRSEIQEYIVGDSGYPLLSYLVTPYDGKELSTSKAEFNKRHTATRL 300
DKGERLNGKR ELPDRSEI+EYI+GDSGYPLL YLVTPYDGKELSTSK EFNKRH TRL
Sbjct: 241 DKGERLNGKRFELPDRSEIREYIIGDSGYPLLPYLVTPYDGKELSTSKTEFNKRHKETRL 300
Query: 301 VVQQALAMLKERWRIIQGVMWRPDKHRLPRIILVCCLLHNIIIDIGDETEDGDVPLSIEH 360
VVQ+ALAMLKERWRIIQGVMWRPDKHRLPRIILVCCLLHNIIIDIGDETE+G+VPLSIEH
Sbjct: 301 VVQRALAMLKERWRIIQGVMWRPDKHRLPRIILVCCLLHNIIIDIGDETEEGEVPLSIEH 360
Query: 361 DVDYKQQVCDVFDSKGAYLRERLSLLFI 389
DVDYKQQVCDVFDSKGAY+R+RLSLLFI
Sbjct: 361 DVDYKQQVCDVFDSKGAYVRDRLSLLFI 388
BLAST of Cmc12g0317571 vs. ExPASy TrEMBL
Match:
A0A6J1K643 (protein ALP1-like OS=Cucurbita maxima OX=3661 GN=LOC111492660 PE=3 SV=1)
HSP 1 Score: 691.4 bits (1783), Expect = 2.1e-195
Identity = 339/389 (87.15%), Postives = 358/389 (92.03%), Query Frame = 0
Query: 1 MGPIRGLRKKKKLERKLDSN-GTASDSSEKDDAIDWWDDFSKRTNGLHSASKGLDRFKSI 60
MGPIRG RKKKKLERKLD+N TASDSSEKDDA+DWWDDFS+RT GLHS +GLD FKSI
Sbjct: 1 MGPIRGSRKKKKLERKLDANASTASDSSEKDDALDWWDDFSRRTIGLHSELEGLDGFKSI 60
Query: 61 FKVSRKTFDYICLLVKDDMTAKSGHFTFLNGRPLSLCDQVAVALRRLGSGESLVTIGDSL 120
FKVSRKTFDYICLLVKDDMTAKS +F FLNGRPLSL DQVAVALRRLGSG+SLVTIG S
Sbjct: 61 FKVSRKTFDYICLLVKDDMTAKSSNFMFLNGRPLSLYDQVAVALRRLGSGDSLVTIGYSF 120
Query: 121 GLNHSTVSQVTWRFVESMEERGLCHLHWPSNEVEMAQVKSKFEKIQGLPNCCGSIDTTHI 180
GLNHSTVSQVTWRFVESME RGL HLHWPS E EMAQVK KFEKIQGLPNCCGSIDTTHI
Sbjct: 121 GLNHSTVSQVTWRFVESMEVRGLRHLHWPSTEEEMAQVKLKFEKIQGLPNCCGSIDTTHI 180
Query: 181 TMCLPASDPTSFVWLDDEKNHSMVLQVIVDAEMRFRDILTGLPGKLSDRLVFQSSNFHKL 240
TMCLP DPTS VWLD EKNHSMVLQVIVDAEMRFRDI+TGLPGK+SD LVFQSSNFHKL
Sbjct: 181 TMCLPVLDPTSNVWLDAEKNHSMVLQVIVDAEMRFRDIITGLPGKMSDWLVFQSSNFHKL 240
Query: 241 CDKGERLNGKRLELPDRSEIQEYIVGDSGYPLLSYLVTPYDGKELSTSKAEFNKRHTATR 300
C+KGERLNGKRLE +RSEI+EYI+GDSGYPLL YLVTPYDGKEL SKAEFNKRHT TR
Sbjct: 241 CEKGERLNGKRLEFINRSEIREYIIGDSGYPLLPYLVTPYDGKELQPSKAEFNKRHTETR 300
Query: 301 LVVQQALAMLKERWRIIQGVMWRPDKHRLPRIILVCCLLHNIIIDIGDETEDGDVPLSIE 360
LVVQ+ALA LKERWRIIQGVMWRPDKHRLPRIILVCCLLHNIIID+GDE EDG+VP+SIE
Sbjct: 301 LVVQRALASLKERWRIIQGVMWRPDKHRLPRIILVCCLLHNIIIDVGDEMEDGNVPMSIE 360
Query: 361 HDVDYKQQVCDVFDSKGAYLRERLSLLFI 389
HD DYKQQ+CDV+DSKGAYLRE+LSLLFI
Sbjct: 361 HDADYKQQICDVYDSKGAYLREKLSLLFI 389
BLAST of Cmc12g0317571 vs. TAIR 10
Match:
AT3G55350.1 (PIF / Ping-Pong family of plant transposases )
HSP 1 Score: 454.9 bits (1169), Expect = 6.4e-128
Identity = 224/403 (55.58%), Postives = 294/403 (72.95%), Query Frame = 0
Query: 1 MGPIRGLRKKKKLERKLDSN--------------GTASDSSEKDD-----AIDWWDDFSK 60
MGPI+ ++KKK+ E+K+D N A ++++ DD ++DWWD FS+
Sbjct: 1 MGPIKTIKKKKRAEKKVDRNVLLAATAAATSASAAAALNNNDDDDDSSSQSLDWWDGFSR 60
Query: 61 RTNGLHSASKGLDRFKSIFKVSRKTFDYICLLVKDDMTAKSGHFTFLNGRPLSLCDQVAV 120
R ++ S F+S+FK+SRKTFDYIC LVK D TAK +F+ NG PLSL D+VAV
Sbjct: 61 R---IYGGSTDPKTFESVFKISRKTFDYICSLVKADFTAKPANFSDSNGNPLSLNDRVAV 120
Query: 121 ALRRLGSGESLVTIGDSLGLNHSTVSQVTWRFVESMEERGLCHLHWPSNEVEMAQVKSKF 180
ALRRLGSGESL IG++ G+N STVSQ+TWRFVESMEER + HL WPS ++ ++KSKF
Sbjct: 121 ALRRLGSGESLSVIGETFGMNQSTVSQITWRFVESMEERAIHHLSWPS---KLDEIKSKF 180
Query: 181 EKIQGLPNCCGSIDTTHITMCLPASDPTSFVWLDDEKNHSMVLQVIVDAEMRFRDILTGL 240
EKI GLPNCCG+ID THI M LPA +P++ VWLD EKN SM LQ +VD +MRF D++ G
Sbjct: 181 EKISGLPNCCGAIDITHIVMNLPAVEPSNKVWLDGEKNFSMTLQAVVDPDMRFLDVIAGW 240
Query: 241 PGKLSDRLVFQSSNFHKLCDKGERLNGKRLELPDRSEIQEYIVGDSGYPLLSYLVTPYDG 300
PG L+D +V ++S F+KL +KG+RLNG++L L +R+E++EYIVGDSG+PLL +L+TPY G
Sbjct: 241 PGSLNDDVVLKNSGFYKLVEKGKRLNGEKLPLSERTELREYIVGDSGFPLLPWLLTPYQG 300
Query: 301 KELSTSKAEFNKRHTATRLVVQQALAMLKERWRIIQGVMWRPDKHRLPRIILVCCLLHNI 360
K S + EFNKRH+ Q AL+ LK+RWRII GVMW PD++RLPRII VCCLLHNI
Sbjct: 301 KPTSLPQTEFNKRHSEATKAAQMALSKLKDRWRIINGVMWMPDRNRLPRIIFVCCLLHNI 360
Query: 361 IIDIGDETEDGDVPLSIEHDVDYKQQVCDVFDSKGAYLRERLS 385
IID+ D+T D D PLS +HD++Y+Q+ C + D + LR+ LS
Sbjct: 361 IIDMEDQTLD-DQPLSQQHDMNYRQRSCKLADEASSVLRDELS 396
BLAST of Cmc12g0317571 vs. TAIR 10
Match:
AT3G63270.1 (CONTAINS InterPro DOMAIN/s: Putative harbinger transposase-derived nuclease (InterPro:IPR006912); BEST Arabidopsis thaliana protein match is: PIF / Ping-Pong family of plant transposases (TAIR:AT3G55350.1); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink). )
HSP 1 Score: 329.3 bits (843), Expect = 4.1e-90
Identity = 180/393 (45.80%), Postives = 243/393 (61.83%), Query Frame = 0
Query: 1 MGPIRGLRKKKKLERKLDSNGTASDSSEK---------DDAI--DWWDDFSKRTNGLHSA 60
M P++ +KKK ++ LD + + EK +AI DWWD F R +
Sbjct: 1 MAPVK--QKKKNKKKPLDKAKKLAKNKEKKRVNAVPLDPEAIDCDWWDTFWLRNSSPSVP 60
Query: 61 SKGLDRFKSIFKVSRKTFDYICLLVKDDMTAK--SGHFTFLNGRPLSLCDQVAVALRRLG 120
S FK F+ S+ TF YIC LV++D+ ++ SG + GR LS+ QVA+ALRRL
Sbjct: 61 SDEDYAFKHFFRASKTTFSYICSLVREDLISRPPSGLIN-IEGRLLSVEKQVAIALRRLA 120
Query: 121 SGESLVTIGDSLGLNHSTVSQVTWRFVESMEERGLCHLHWPSNEVEMAQVKSKFEKIQGL 180
SG+S V++G + G+ STVSQVTWRF+E++EER HL WP ++ + ++KSKFE++ GL
Sbjct: 121 SGDSQVSVGAAFGVGQSTVSQVTWRFIEALEERAKHHLRWPDSD-RIEEIKSKFEEMYGL 180
Query: 181 PNCCGSIDTTHITMCLPASDPTSFVWLDDEKNHSMVLQVIVDAEMRFRDILTGLPGKLSD 240
PNCCG+IDTTHI M LPA S W D EKN+SM LQ + D EMRF +++TG PG ++
Sbjct: 181 PNCCGAIDTTHIIMTLPAVQ-ASDDWCDQEKNYSMFLQGVFDHEMRFLNMVTGWPGGMTV 240
Query: 241 RLVFQSSNFHKLCDKGERLNGKRLELPDRSEIQEYIVGDSGYPLLSYLVTPYDGKELSTS 300
+ + S F KLC+ + L+G L ++I+EY+VG YPLL +L+TP+D S S
Sbjct: 241 SKLLKFSGFFKLCENAQILDGNPKTLSQGAQIREYVVGGISYPLLPWLITPHDSDHPSDS 300
Query: 301 KAEFNKRHTATRLVVQQALAMLKERWRIIQGVMWRPDKHRLPRIILVCCLLHNIIIDIGD 360
FN+RH R V A LK WRI+ VMWRPD+ +LP IILVCCLLHNIIID GD
Sbjct: 301 MVAFNERHEKVRSVAATAFQQLKGSWRILSKVMWRPDRRKLPSIILVCCLLHNIIIDCGD 360
Query: 361 ETEDGDVPLSIEHDVDYKQQVCDVFDSKGAYLR 381
++ DVPLS HD Y + C + G+ LR
Sbjct: 361 YLQE-DVPLSGHHDSGYADRYCKQTEPLGSELR 387
BLAST of Cmc12g0317571 vs. TAIR 10
Match:
AT5G12010.1 (unknown protein; INVOLVED IN: response to salt stress; LOCATED IN: chloroplast, plasma membrane, membrane; EXPRESSED IN: 23 plant structures; EXPRESSED DURING: 13 growth stages; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT4G29780.1); Has 1807 Blast hits to 1807 proteins in 277 species: Archae - 0; Bacteria - 0; Metazoa - 736; Fungi - 347; Plants - 385; Viruses - 0; Other Eukaryotes - 339 (source: NCBI BLink). )
HSP 1 Score: 130.6 bits (327), Expect = 2.8e-30
Identity = 82/319 (25.71%), Postives = 154/319 (48.28%), Query Frame = 0
Query: 29 KDDAIDWWDDFSKRTNGLHSASKGLDRFKSIFKVSRKTFDYICLLVKDDMTAKSGHFTFL 88
KD + WW++ S+ + FK F++S+ TF+ IC D++ +
Sbjct: 155 KDRSRAWWEECSR-------LDYPEEDFKKAFRMSKSTFELIC----DELNSAVAKEDTA 214
Query: 89 NGRPLSLCDQVAVALRRLGSGESLVTIGDSLGLNHSTVSQVTWRFVESMEERGL-CHLHW 148
+ + +VAV + RL +GE L + GL ST ++ +++++ + +L W
Sbjct: 215 LRNAIPVRQRVAVCIWRLATGEPLRLVSKKFGLGISTCHKLVLEVCKAIKDVLMPKYLQW 274
Query: 149 PSNEVEMAQVKSKFEKIQGLPNCCGSIDTTHITMCLPASDPTSF-----VWLDDEKNHSM 208
P +E + ++ +FE + G+PN GS+ TTHI + P S+ + + ++S+
Sbjct: 275 PDDE-SLRNIRERFESVSGIPNVVGSMYTTHIPIIAPKISVASYFNKRHTERNQKTSYSI 334
Query: 209 VLQVIVDAEMRFRDILTGLPGKLSDRLVFQSSNFHKLCDKGERLNGKRLELPDRSEIQEY 268
+Q +V+ + F D+ G PG + D V + S ++ + G L G +
Sbjct: 335 TIQAVVNPKGVFTDLCIGWPGSMPDDKVLEKSLLYQRANNGGLLKGM------------W 394
Query: 269 IVGDSGYPLLSYLVTPYDGKELSTSKAEFNKRHTATRLVVQQALAMLKERWRIIQGVMWR 328
+ G G+PLL +++ PY + L+ ++ FN++ + + V ++A LK RW +Q
Sbjct: 395 VAGGPGHPLLDWVLVPYTQQNLTWTQHAFNEKMSEVQGVAKEAFGRLKGRWACLQ-KRTE 448
Query: 329 PDKHRLPRIILVCCLLHNI 342
LP ++ CC+LHNI
Sbjct: 455 VKLQDLPTVLGACCVLHNI 448
BLAST of Cmc12g0317571 vs. TAIR 10
Match:
AT4G29780.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT5G12010.1); Has 945 Blast hits to 944 proteins in 87 species: Archae - 0; Bacteria - 0; Metazoa - 519; Fungi - 43; Plants - 365; Viruses - 0; Other Eukaryotes - 18 (source: NCBI BLink). )
HSP 1 Score: 122.5 bits (306), Expect = 7.6e-28
Identity = 91/319 (28.53%), Postives = 151/319 (47.34%), Query Frame = 0
Query: 29 KDDAIDWWDDFSKRTNGLHSASKGLDRFKSIFKVSRKTFDYICLLVKDDMTAKSGHFTFL 88
K+ DWWD S+ D F+ F++S+ TF+ IC + +T K+ T L
Sbjct: 193 KERTTDWWDRVSR-------PDFPEDEFRREFRMSKSTFNLICEELDTTVTKKN---TML 252
Query: 89 NGRPLSLCDQVAVALRRLGSGESLVTIGDSLGLNHSTVSQVTWRFVESMEERGL-CHLHW 148
+ +V V + RL +G L + + GL ST ++ ++ + + +L W
Sbjct: 253 RD-AIPAPKRVGVCVWRLATGAPLRHVSERFGLGISTCHKLVIEVCRAIYDVLMPKYLLW 312
Query: 149 PSNEVEMAQVKSKFEKIQGLPNCCGSIDTTHITMCLPASDPTSF-----VWLDDEKNHSM 208
PS+ E+ K+KFE + +PN GSI TTHI + P ++ + + ++S+
Sbjct: 313 PSDS-EINSTKAKFESVHKIPNVVGSIYTTHIPIIAPKVHVAAYFNKRHTERNQKTSYSI 372
Query: 209 VLQVIVDAEMRFRDILTGLPGKLSDRLVFQSSNFHKLCDKGERLNGKRLELPDRSEIQEY 268
+Q +V+A+ F D+ G PG L+D + + S+ R R L D +
Sbjct: 373 TVQGVVNADGIFTDVCIGNPGSLTDDQILEKSSL-------SRQRAARGMLRD-----SW 432
Query: 269 IVGDSGYPLLSYLVTPYDGKELSTSKAEFNKRHTATRLVVQQALAMLKERWRIIQGVMWR 328
IVG+SG+PL YL+ PY + L+ ++ FN+ + + A LK RW +Q
Sbjct: 433 IVGNSGFPLTDYLLVPYTRQNLTWTQHAFNESIGEIQGIATAAFERLKGRWACLQ-KRTE 486
Query: 329 PDKHRLPRIILVCCLLHNI 342
LP ++ CC+LHNI
Sbjct: 493 VKLQDLPYVLGACCVLHNI 486
BLAST of Cmc12g0317571 vs. TAIR 10
Match:
AT3G19120.1 (PIF / Ping-Pong family of plant transposases )
HSP 1 Score: 102.8 bits (255), Expect = 6.2e-22
Identity = 82/296 (27.70%), Postives = 138/296 (46.62%), Query Frame = 0
Query: 55 RFKSIFKVSRKTFDYICLLVKDDMTAKSGHFTFLNGRPLSLCDQVAVA--LRRLGSGESL 114
R++S++ +S F + +K +TA + LSL AVA L RL G S
Sbjct: 116 RWRSLYGLSYPVFITVVDKLKPFITASN----------LSLPADYAVAMVLSRLAHGCSA 175
Query: 115 VTIGDSLGLNHSTVSQVTWRFVESMEERGLC------HLHWPSNEVEMAQVKSKFEKIQG 174
T+ L+ +S++T +M R L + P + + + FE++
Sbjct: 176 KTLASRYSLDPYLISKIT-----NMVTRLLATKLYPEFIKIPVGKRRLIETTQGFEELTS 235
Query: 175 LPNCCGSIDTTHITMCLPASDPTSFVWLDDEKNHSMVLQVIVDAEMRFRDILTGLPGKLS 234
LPN CG+ID+T + + ++ +++LQV+ D + F D+ PG
Sbjct: 236 LPNICGAIDSTPVKLRRRTKLNPRNIYGCKYGYDAVLLQVVADHKKIFWDVCVKAPGGED 295
Query: 235 DRLVFQSSNFHKLCDKGERLNGKRLELPDRSEIQEYIVGDSGYPLLSYLVTPYDGKELST 294
D F+ S +K G+ + K + + ++ YIVGD YPLLS+L+TP+ T
Sbjct: 296 DSSHFRDSLLYKRLTSGDIVWEKVINIRGH-HVRPYIVGDWCYPLLSFLMTPFSPNGSGT 355
Query: 295 SKAE-FNKRHTATRLVVQQALAMLKERWRIIQGVMWRPDKHRLPRIILVCCLLHNI 342
F+ R VV +A+ +LK RW+I+Q + + P+ I+ CC+LHN+
Sbjct: 356 PPENLFDGMLMKGRSVVVEAIGLLKARWKILQSL--NVGVNHAPQTIVACCVLHNL 393
The following BLAST results are available for this feature:
Match Name | E-value | Identity | Description | |
KAA0033909.1 | 3.7e-226 | 100.00 | putative nuclease HARBI1 [Cucumis melo var. makuwa] | [more] |
TYK01291.1 | 9.1e-225 | 99.74 | putative nuclease HARBI1 [Cucumis melo var. makuwa] | [more] |
XP_008457540.1 | 9.1e-225 | 99.48 | PREDICTED: LOW QUALITY PROTEIN: putative nuclease HARBI1 [Cucumis melo] | [more] |
XP_004149039.1 | 5.3e-217 | 95.62 | protein ALP1-like [Cucumis sativus] >KGN65671.1 hypothetical protein Csa_019843 ... | [more] |
XP_038893506.1 | 3.6e-213 | 94.86 | protein ALP1-like [Benincasa hispida] | [more] |
Match Name | E-value | Identity | Description | |
Q9M2U3 | 9.1e-127 | 55.58 | Protein ALP1-like OS=Arabidopsis thaliana OX=3702 GN=At3g55350 PE=2 SV=1 | [more] |
Q94K49 | 5.7e-89 | 45.80 | Protein ANTAGONIST OF LIKE HETEROCHROMATIN PROTEIN 1 OS=Arabidopsis thaliana OX=... | [more] |
Q6AZB8 | 1.6e-27 | 27.74 | Putative nuclease HARBI1 OS=Danio rerio OX=7955 GN=harbi1 PE=2 SV=1 | [more] |
Q8BR93 | 2.4e-26 | 27.09 | Putative nuclease HARBI1 OS=Mus musculus OX=10090 GN=Harbi1 PE=2 SV=1 | [more] |
B0BN95 | 2.4e-26 | 26.42 | Putative nuclease HARBI1 OS=Rattus norvegicus OX=10116 GN=Harbi1 PE=2 SV=1 | [more] |
Match Name | E-value | Identity | Description | |
A0A5A7SXL0 | 1.8e-226 | 100.00 | Putative nuclease HARBI1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffol... | [more] |
A0A5D3BNB9 | 4.4e-225 | 99.74 | Putative nuclease HARBI1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffol... | [more] |
A0A1S3C6F2 | 4.4e-225 | 99.48 | LOW QUALITY PROTEIN: putative nuclease HARBI1 OS=Cucumis melo OX=3656 GN=LOC1034... | [more] |
A0A0A0M0C2 | 2.6e-217 | 95.62 | DDE Tnp4 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G481730 PE... | [more] |
A0A6J1K643 | 2.1e-195 | 87.15 | protein ALP1-like OS=Cucurbita maxima OX=3661 GN=LOC111492660 PE=3 SV=1 | [more] |
Match Name | E-value | Identity | Description | |
AT3G55350.1 | 6.4e-128 | 55.58 | PIF / Ping-Pong family of plant transposases | [more] |
AT3G63270.1 | 4.1e-90 | 45.80 | CONTAINS InterPro DOMAIN/s: Putative harbinger transposase-derived nuclease (Int... | [more] |
AT5G12010.1 | 2.8e-30 | 25.71 | unknown protein; INVOLVED IN: response to salt stress; LOCATED IN: chloroplast, ... | [more] |
AT4G29780.1 | 7.6e-28 | 28.53 | unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TA... | [more] |
AT3G19120.1 | 6.2e-22 | 27.70 | PIF / Ping-Pong family of plant transposases | [more] |