Cmc11g0309801 (gene) Melon (Charmono) v1.1

Overview
NameCmc11g0309801
Typegene
OrganismCucumis melo L. var. cantalupensis cv. Charmono (Melon (Charmono) v1.1)
Descriptionabietadienol/abietadienal oxidase isoform X1
LocationCMiso1.1chr11: 30397930 .. 30400174 (-)
RNA-Seq ExpressionCmc11g0309801
SyntenyCmc11g0309801
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GGCAAAAGTCAGACAGAGAGAAAGAAAATGATCATTTTGAAAATAAAGAAAAGACAGAAATAATAGAGAAAGGGGAGAGAAATAGAGAAATGATCATATCATAAAAATCAACTTATTGGAGAGATTGGAATTAATGCTTAAAATGGATTGAAGAAGTTGATAGCCAAAAGCTTTAAACTTTGTAAGTTCTCTCTCTCTCTCTCTCTCTCTCTAAATGTTCTAAATATATTATGTAAAAGAAGCTAAGAAATAAATATATGTAAGTTTTCAAAACTTTGATGTTGAGTTTTTTCTTTTTTTTCCTTTTTGGATTTTGTTGATAAGAGTTCAATTATATAATTGATGAGTTCATCGTTGTTTTAAGCTAATTCTATTGAACATTACAATTTTTTTTAGTTCGAGAATATGTGATGTGTAGGATCTAAACTTCTGACTTTTTGGTCAAAGTTACGTACTTAATATCGATAAAACATGAATGAAATGACTTATAAGTTTTTTGTTTTTTTTTTCTTACTAAAAAGTTTTTGTTTCTATGTTCTTGCCTCGAAATGTTGATAAACATTAGAAATTTATCGTGAAAATGGAAGAGGAAAATAAACGAAAGAGAAAGATGAAAAGAGTGGAAAAGGAAGAAGAAAGTAAATAATGAAAAAGGGATACATCTTTACATGCATGATTAGAGATGATTTATTCATTTTTGTACTATGGAAAATGGTTGTATGGTTACTAAGTTAGAATTAATGATTTAGTTTCTTTAAAACATTCGAGTTATATATTACCGAAAAAGATGTGTTTGTATGTTAGCCACGTATTAAAATTTTATATATAGCATTTTAGTTCGAAGTTAAAACCTTTGCAAAATAATTGTGTCCTATGAATTATGTCAATGTCACATCATGCATCATACGATGAAAGTACTCGTAGTTCTAAATGATTTGGAAGAAGAAGCATTTTTCCCCTTTTTGTATTCTAGCCAATTGATATTTTTATTCTTTAGTTCATATATGATTAATTTAAACTGTTACAAGTATCACTTTCAATAAATAATTGGAATAACTTTTTAAGTGATTCATTTCAAATTATTTCTAAATTTTTTTTAGTATGTTAGCTTTGAAATTTTGCAATTTATCTTAGCCAGGTCGAAGATGACAATGTGGGAGTCCTCCTTGGTCATGTGGTCATTTTTCATATTTACACTATTGTCCGCTACTTTTCTTTCAAACATATTCAAAAATAAAAAGTACAACAATAACCAGAACGATCAAAGTCAAACAGGATACAAATTTCCTCCGGAGAGAAGAAGCTGGCCTCTTATAGGTGATAGCTTCGACTGGTATTCTGCCATTGCAAGTTCTCATCCTTCCAAATATGTTGAAGAACAAGCCAAAAGGTAATATAACTTAAAAACAAAGACCTATACGTACGTACGTATACAGACACATATATACTCTTTTTCTTTACTTTTTTAACTTTGTCATTTGTTTCAAAAGTTTCGGTATTTTTTTCAATAGTTACAATATTACTTTTGACATTCTATAAAAGTTTTTCAAAATAACTCTTCAAATACAAGTTTGTTAGAATGTCGAAATGTATTGCAATTCAAAAAAATTTCCTAAGCGTAATTAATTATGTACATAATTTATCTTCTTTTTTTTTTCTTTCTTTGGGTTAAGTTTTCTATAAAACATATGATATGATGTCGTCTTAACCAATTGAGCTGACCTAAAAAGATACGGGAAGATCTTCTCGTGTCGTGTTTTTGGTAAATGTACAGTGGTATCCGTCGATCCAGAGTTTAATAGATATGTGATGCAAAACGAAGGGATATTGTTTCAATCGAGCTATCCCAAATCTTTTCGAGATTTGGTAGGCGTCAATGGTGTTATCACAGCTCAAGGAGAACAACAAAGGAAACTCCATGGAATTGCGTCCAATTTTATGCGTATAAACAAACTCAACTTTAGCTTCCTAAAGGAAATTCAAACCATAATCATCCATTCCTTGACCACTTTCCATGACCGTCATCAAATCATCTCTCTCCAAGATGCTTGCAGGAAGGCTAGTATGCATACATATATATATTATTTGTTACGTTATTACGAGTTTAATTATGTCGTCTTGATTAATAATCATTTAATTTTTCACGTAAAAATGGTATTTGAACTATTCTTAGACAACTTCTTATTCTAGGAGAAAAAAAAAACTTTTTAACAACTTTTTCCTTTTTTTTTTTCATAATTTGGCT

mRNA sequence

GGCAAAAGTCAGACAGAGAGAAAGAAAATGATCATTTTGAAAATAAAGAAAAGACAGAAATAATAGAGAAAGGGGAGAGAAATAGAGAAATGATCATATCATAAAAATCAACTTATTGGAGAGATTGGAATTAATGCTTAAAATGGATTGAAGAAGTTGATAGCCAAAAGCTTTAAACTTTGTCGAAGATGACAATGTGGGAGTCCTCCTTGGTCATGTGGTCATTTTTCATATTTACACTATTGTCCGCTACTTTTCTTTCAAACATATTCAAAAATAAAAAGTACAACAATAACCAGAACGATCAAAGTCAAACAGGATACAAATTTCCTCCGGAGAGAAGAAGCTGGCCTCTTATAGGTGATAGCTTCGACTGGTATTCTGCCATTGCAAGTTCTCATCCTTCCAAATATGTTGAAGAACAAGCCAAAAGATACGGGAAGATCTTCTCGTGTCGTGTTTTTGGTAAATGTACAGTGGTATCCGTCGATCCAGAGTTTAATAGATATGTGATGCAAAACGAAGGGATATTGTTTCAATCGAGCTATCCCAAATCTTTTCGAGATTTGGTAGGCGTCAATGGTGTTATCACAGCTCAAGGAGAACAACAAAGGAAACTCCATGGAATTGCGTCCAATTTTATGCGTATAAACAAACTCAACTTTAGCTTCCTAAAGGAAATTCAAACCATAATCATCCATTCCTTGACCACTTTCCATGACCGTCATCAAATCATCTCTCTCCAAGATGCTTGCAGGAAGGCTAGTATGCATACATATATATATTATTTGTTACGTTATTACGAGTTTAATTATGTCGTCTTGATTAATAATCATTTAATTTTTCACGTAAAAATGGTATTTGAACTATTCTTAGACAACTTCTTATTCTAGGAGAAAAAAAAAACTTTTTAACAACTTTTTCCTTTTTTTTTTTCATAATTTGGCT

Coding sequence (CDS)

ATGACAATGTGGGAGTCCTCCTTGGTCATGTGGTCATTTTTCATATTTACACTATTGTCCGCTACTTTTCTTTCAAACATATTCAAAAATAAAAAGTACAACAATAACCAGAACGATCAAAGTCAAACAGGATACAAATTTCCTCCGGAGAGAAGAAGCTGGCCTCTTATAGGTGATAGCTTCGACTGGTATTCTGCCATTGCAAGTTCTCATCCTTCCAAATATGTTGAAGAACAAGCCAAAAGATACGGGAAGATCTTCTCGTGTCGTGTTTTTGGTAAATGTACAGTGGTATCCGTCGATCCAGAGTTTAATAGATATGTGATGCAAAACGAAGGGATATTGTTTCAATCGAGCTATCCCAAATCTTTTCGAGATTTGGTAGGCGTCAATGGTGTTATCACAGCTCAAGGAGAACAACAAAGGAAACTCCATGGAATTGCGTCCAATTTTATGCGTATAAACAAACTCAACTTTAGCTTCCTAAAGGAAATTCAAACCATAATCATCCATTCCTTGACCACTTTCCATGACCGTCATCAAATCATCTCTCTCCAAGATGCTTGCAGGAAGGCTAGTATGCATACATATATATATTATTTGTTACGTTATTACGAGTTTAATTATGTCGTCTTGATTAATAATCATTTAATTTTTCACGTAAAAATGGTATTTGAACTATTCTTAGACAACTTCTTATTCTAG

Protein sequence

MTMWESSLVMWSFFIFTLLSATFLSNIFKNKKYNNNQNDQSQTGYKFPPERRSWPLIGDSFDWYSAIASSHPSKYVEEQAKRYGKIFSCRVFGKCTVVSVDPEFNRYVMQNEGILFQSSYPKSFRDLVGVNGVITAQGEQQRKLHGIASNFMRINKLNFSFLKEIQTIIIHSLTTFHDRHQIISLQDACRKASMHTYIYYLLRYYEFNYVVLINNHLIFHVKMVFELFLDNFLF
Homology
BLAST of Cmc11g0309801 vs. NCBI nr
Match: XP_016902261.1 (PREDICTED: abietadienol/abietadienal oxidase isoform X1 [Cucumis melo])

HSP 1 Score: 387.1 bits (993), Expect = 1.1e-103
Identity = 191/202 (94.55%), Postives = 196/202 (97.03%), Query Frame = 0

Query: 1   MTMWESSLVMWSFFIFTLLSATFLSNIFKNKKYNNNQNDQSQTGYKFPPERRSWPLIGDS 60
           MTMWESSLV+WSFFIFTLLSATFLSNIFKNKKYNNNQNDQSQTGYKFPP RRSWPLIGDS
Sbjct: 1   MTMWESSLVVWSFFIFTLLSATFLSNIFKNKKYNNNQNDQSQTGYKFPPGRRSWPLIGDS 60

Query: 61  FDWYSAIASSHPSKYVEEQAKRYGKIFSCRVFGKCTVVSVDPEFNRYVMQNEGILFQSSY 120
           FDWYSAIASSHPSKYVEEQAKRYGKIFSCRVFGKCTVVSVDPEFNRYVMQNEGILFQSSY
Sbjct: 61  FDWYSAIASSHPSKYVEEQAKRYGKIFSCRVFGKCTVVSVDPEFNRYVMQNEGILFQSSY 120

Query: 121 PKSFRDLVGVNGVITAQGEQQRKLHGIASNFMRINKLNFSFLKEIQTIIIHSLTTFHDRH 180
           PKSFRDLVGVNGVITAQGEQQRKLHGIASNFMRINKLNFSFLKEIQTIIIHSLTTFHDRH
Sbjct: 121 PKSFRDLVGVNGVITAQGEQQRKLHGIASNFMRINKLNFSFLKEIQTIIIHSLTTFHDRH 180

Query: 181 QIISLQDACRKASMHTYIYYLL 203
           QIISLQDACRK +++  +  LL
Sbjct: 181 QIISLQDACRKIAINVMVSQLL 202

BLAST of Cmc11g0309801 vs. NCBI nr
Match: XP_031742587.1 (abietadienol/abietadienal oxidase [Cucumis sativus] >XP_031742588.1 abietadienol/abietadienal oxidase [Cucumis sativus] >KGN45630.2 hypothetical protein Csa_004868 [Cucumis sativus])

HSP 1 Score: 348.6 bits (893), Expect = 4.2e-92
Identity = 174/202 (86.14%), Postives = 184/202 (91.09%), Query Frame = 0

Query: 3   MWESSLVMWSFFIFTLLSATFLSNIFKNKKYN--NNQNDQSQTGYKFPPERRSWPLIGDS 62
           MWE SLV WSFFI TLL  TFL NI K KKYN  N+QNDQS+T YKFP  RRSWP+IGDS
Sbjct: 1   MWEPSLVAWSFFILTLLFTTFLPNILKKKKYNDQNDQNDQSRTRYKFPSGRRSWPVIGDS 60

Query: 63  FDWYSAIASSHPSKYVEEQAKRYGKIFSCRVFGKCTVVSVDPEFNRYVMQNEGILFQSSY 122
           F+WYSAIASSHPSKYVEEQAKRYGKIFSCRVFGKCTVVSVDP+FNRYVMQNEGILFQSSY
Sbjct: 61  FNWYSAIASSHPSKYVEEQAKRYGKIFSCRVFGKCTVVSVDPDFNRYVMQNEGILFQSSY 120

Query: 123 PKSFRDLVGVNGVITAQGEQQRKLHGIASNFMRINKLNFSFLKEIQTIIIHSLTTFHDRH 182
           PKSFRDLVGVNGVITAQGEQQRKLHGIASNFMRINKLNFSF+KEIQTIIIHSLTTFHDRH
Sbjct: 121 PKSFRDLVGVNGVITAQGEQQRKLHGIASNFMRINKLNFSFIKEIQTIIIHSLTTFHDRH 180

Query: 183 QIISLQDACRKASMHTYIYYLL 203
           QIISLQDACRK +++  +  LL
Sbjct: 181 QIISLQDACRKIAINLMVSQLL 202

BLAST of Cmc11g0309801 vs. NCBI nr
Match: XP_038875678.1 (abietadienol/abietadienal oxidase [Benincasa hispida])

HSP 1 Score: 312.0 bits (798), Expect = 4.4e-81
Identity = 156/200 (78.00%), Postives = 172/200 (86.00%), Query Frame = 0

Query: 3   MWESSLVMWSFFIFTLLSATFLSNIFKNKKYNNNQNDQSQTGYKFPPERRSWPLIGDSFD 62
           MWE S  MW+FF+ TLLS TFLS I K     N+QNDQSQ    FPP RRSWP+IGDSF+
Sbjct: 1   MWEPSCEMWTFFMLTLLSITFLSKILK-----NHQNDQSQRRNTFPPGRRSWPVIGDSFN 60

Query: 63  WYSAIASSHPSKYVEEQAKRYGKIFSCRVFGKCTVVSVDPEFNRYVMQNEGILFQSSYPK 122
           WYSAIASSHPSKYVEEQAKRYGKIFSC VFGK TVVS+DP+FNRYVMQNEG LFQSSYPK
Sbjct: 61  WYSAIASSHPSKYVEEQAKRYGKIFSCHVFGKYTVVSIDPKFNRYVMQNEGTLFQSSYPK 120

Query: 123 SFRDLVGVNGVITAQGEQQRKLHGIASNFMRINKLNFSFLKEIQTIIIHSLTTFHDRHQI 182
           SFRDLVG+NGVIT QGEQQRKLH IASNFMRINKL+FSFLK+IQTIIIHSL+TFHD HQ+
Sbjct: 121 SFRDLVGLNGVITVQGEQQRKLHAIASNFMRINKLDFSFLKDIQTIIIHSLSTFHDGHQV 180

Query: 183 ISLQDACRKASMHTYIYYLL 203
           ISLQDACRK +++  +  LL
Sbjct: 181 ISLQDACRKIAINMMVSQLL 195

BLAST of Cmc11g0309801 vs. NCBI nr
Match: XP_022158708.1 (abietadienol/abietadienal oxidase isoform X1 [Momordica charantia])

HSP 1 Score: 257.7 bits (657), Expect = 9.8e-65
Identity = 137/207 (66.18%), Postives = 157/207 (75.85%), Query Frame = 0

Query: 3   MWES-SLVMWSFF-IFTL-LSATFLSNIFKNKKYNNNQNDQSQTGYKFPPERRSWPLIGD 62
           MWE  + V+WS   I TL LS  FL+   KNKKY     D+ Q  Y+FPP RRSWP+IGD
Sbjct: 1   MWEQPASVLWSLLSILTLMLSILFLARTLKNKKYREG-GDEDQRAYRFPPGRRSWPVIGD 60

Query: 63  SFDWYSAIASSHPSKYVEEQAKRYGKIFSCRVFGKCTVVSVDPEFNRYVMQNEGILFQSS 122
           SF+WYSA+ASSHP KYVEEQ KRYGKIFSCRVFGK  VVS DP FNRYVMQNEG LFQSS
Sbjct: 61  SFNWYSAVASSHPPKYVEEQVKRYGKIFSCRVFGKWAVVSADPVFNRYVMQNEGKLFQSS 120

Query: 123 YPKSFRDLVGVNGVITAQGEQQRKLHGIASNFMRINK---LNFSFLKEIQTIIIHSLTTF 182
           YPKSFRDLVG+NGVIT QGEQQRKLH IA N MR++K   LNF FL+ IQTIIIH+L +F
Sbjct: 121 YPKSFRDLVGMNGVITVQGEQQRKLHAIACNMMRVHKHKVLNFPFLQHIQTIIIHTLCSF 180

Query: 183 -HDRHQIISLQDACRKASMHTYIYYLL 203
               HQ++ LQD CRK +++  +  LL
Sbjct: 181 SFPNHQVVYLQDVCRKVAINLMVTQLL 206

BLAST of Cmc11g0309801 vs. NCBI nr
Match: XP_022158712.1 (abietadienol/abietadienal oxidase isoform X4 [Momordica charantia])

HSP 1 Score: 257.7 bits (657), Expect = 9.8e-65
Identity = 137/207 (66.18%), Postives = 157/207 (75.85%), Query Frame = 0

Query: 3   MWES-SLVMWSFF-IFTL-LSATFLSNIFKNKKYNNNQNDQSQTGYKFPPERRSWPLIGD 62
           MWE  + V+WS   I TL LS  FL+   KNKKY     D+ Q  Y+FPP RRSWP+IGD
Sbjct: 1   MWEQPASVLWSLLSILTLMLSILFLARTLKNKKYREG-GDEDQRAYRFPPGRRSWPVIGD 60

Query: 63  SFDWYSAIASSHPSKYVEEQAKRYGKIFSCRVFGKCTVVSVDPEFNRYVMQNEGILFQSS 122
           SF+WYSA+ASSHP KYVEEQ KRYGKIFSCRVFGK  VVS DP FNRYVMQNEG LFQSS
Sbjct: 61  SFNWYSAVASSHPPKYVEEQVKRYGKIFSCRVFGKWAVVSADPVFNRYVMQNEGKLFQSS 120

Query: 123 YPKSFRDLVGVNGVITAQGEQQRKLHGIASNFMRINK---LNFSFLKEIQTIIIHSLTTF 182
           YPKSFRDLVG+NGVIT QGEQQRKLH IA N MR++K   LNF FL+ IQTIIIH+L +F
Sbjct: 121 YPKSFRDLVGMNGVITVQGEQQRKLHAIACNMMRVHKHKVLNFPFLQHIQTIIIHTLCSF 180

Query: 183 -HDRHQIISLQDACRKASMHTYIYYLL 203
               HQ++ LQD CRK +++  +  LL
Sbjct: 181 SFPNHQVVYLQDVCRKVAINLMVTQLL 206

BLAST of Cmc11g0309801 vs. ExPASy Swiss-Prot
Match: Q50EK6 (Abietadienol/abietadienal oxidase OS=Pinus taeda OX=3352 GN=CYP720B1 PE=1 SV=1)

HSP 1 Score: 135.2 bits (339), Expect = 9.6e-31
Identity = 65/178 (36.52%), Postives = 112/178 (62.92%), Query Frame = 0

Query: 26  NIFKNKKYNNNQNDQSQTGYKFPPERRSWPLIGDSFDWYSAIASSHPSKYVEEQAKRY-G 85
           N  + +K  +N+ +Q       PP    WPLIG+++ +Y ++ S+ P ++++++ KRY  
Sbjct: 27  NAQRGQKRTSNEKNQE---LHLPPGSTGWPLIGETYSYYRSMTSNRPRQFIDDREKRYDS 86

Query: 86  KIFSCRVFGKCTVVSVDPEFNRYVMQNEGILFQSSYPKSFRDLVGVNGVITAQGEQQRKL 145
            +F   +FG   V+S DP+FN+YV+QNEG  FQ+ YPK+ + L+G  G+++  G+ QRKL
Sbjct: 87  DVFVSHLFGSQAVISSDPQFNKYVLQNEGRFFQAHYPKALKALIGDYGLLSVHGDLQRKL 146

Query: 146 HGIASNFMRINKLNFSFLKEIQTIIIHSLTTFHDRHQIISLQDACRKASMHTYIYYLL 203
           HGIA N +R  +L F F++EIQ ++  +L  + D+ + I+LQ+ C +  ++     LL
Sbjct: 147 HGIAVNLLRFERLKFDFMEEIQNLVHSTLDRWVDKKE-IALQNECHQMVLNLMAKQLL 200

BLAST of Cmc11g0309801 vs. ExPASy Swiss-Prot
Match: Q50EK5 (Cytochrome P450 720B2 OS=Pinus taeda OX=3352 GN=CYP720B2 PE=2 SV=1)

HSP 1 Score: 130.2 bits (326), Expect = 3.1e-29
Identity = 63/164 (38.41%), Postives = 106/164 (64.63%), Query Frame = 0

Query: 41  SQTGYKFPPERRSWPLIGDSFDWYSAI-ASSHPSKYVEEQAKRYGKIFSCRVFGKC-TVV 100
           S   YK PP    WPLIG++  ++  I +++ P ++++E+ +RYG+IF   +FG+   VV
Sbjct: 38  SSRAYKLPPGSTGWPLIGETISFFRGINSTAQPRQFIQEREQRYGEIFRSNLFGRSRIVV 97

Query: 101 SVDPEFNRYVMQNEGILFQSSYPKSFRDLVGVNGVITAQGEQQRKLHGIASNFMRINKLN 160
           SVDPEFN++V+Q+EG  FQ++YPK  R+L+G  G+++  G+ QRKLHG A N +R  +L+
Sbjct: 98  SVDPEFNKHVLQHEGRQFQANYPKPLRNLIGKYGLLSVHGDLQRKLHGAAVNLLRFERLS 157

Query: 161 FSFLKEIQTIIIHSLTTFHDRHQIISLQDACRKASMHTYIYYLL 203
             F+++IQ ++  +L  +  +   I LQ+ C +  ++     LL
Sbjct: 158 VDFMEDIQNLLHITLAKWEAKRD-IHLQEECHQLVLNLMAKQLL 200

BLAST of Cmc11g0309801 vs. ExPASy Swiss-Prot
Match: Q94IA6 (3-epi-6-deoxocathasterone 23-monooxygenase CYP90D1 OS=Arabidopsis thaliana OX=3702 GN=CYP90D1 PE=1 SV=1)

HSP 1 Score: 103.6 bits (257), Expect = 3.1e-21
Identity = 54/190 (28.42%), Postives = 100/190 (52.63%), Query Frame = 0

Query: 6   SSLVMWSFFIFTLLSA-----TFLSNIFKNKKYNNNQNDQSQTGYKFPPERRSWPLIGDS 65
           SSL+ +SFF F ++          S+    KK N++       G KFP     WP+IG++
Sbjct: 5   SSLLFFSFFFFIIIVIFNKINGLRSSPASKKKLNDHHVTSQSHGPKFPHGSLGWPVIGET 64

Query: 66  FDWYSAIASSHPSKYVEEQAKRYGKIFSCRVFGKCTVVSVDPEFNRYVMQNEGILFQSSY 125
            ++ S+  S  P  +++++   YG++F   +FG  T+VS D E NR V+Q++   F   Y
Sbjct: 65  IEFVSSAYSDRPESFMDKRRLMYGRVFKSHIFGTATIVSTDAEVNRAVLQSDSTAFVPFY 124

Query: 126 PKSFRDLVGVNGVITAQGEQQRKLHGIASNFMRINKLNFSFLKEIQTIIIHSLTTFHDRH 185
           PK+ R+L+G + ++   G   R+ HG+  +F++   L    ++++   +  S+  + +  
Sbjct: 125 PKTVRELMGKSSILLINGSLHRRFHGLVGSFLKSPLLKAQIVRDMHKFLSESMDLWSE-D 184

Query: 186 QIISLQDACR 191
           Q + LQD  +
Sbjct: 185 QPVLLQDVSK 193

BLAST of Cmc11g0309801 vs. ExPASy Swiss-Prot
Match: Q5CCK3 (Cytochrome P450 90B2 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP90B2 PE=1 SV=1)

HSP 1 Score: 97.1 bits (240), Expect = 2.9e-19
Identity = 48/140 (34.29%), Postives = 82/140 (58.57%), Query Frame = 0

Query: 48  PPERRSWPLIGDSFDWYSAIASSHPSKYVEEQAKRYGKIFSCRVFGKCTVVSVDPEFNRY 107
           PP    WPL+G++F +  A  ++   +++E+   RYGKI+   +FG+ TVVS D   NRY
Sbjct: 55  PPGAAGWPLVGETFGYLRAHPATSVGRFMEQHIARYGKIYRSSLFGERTVVSADAGLNRY 114

Query: 108 VMQNEGILFQSSYPKSFRDLVGVNGVITAQGEQQRKLHGIASNFMRINKLNFSFLKEIQ- 167
           ++QNEG LF+ SYP+S   ++G   ++   G+  R++  I+ NF+   +L    L E++ 
Sbjct: 115 ILQNEGRLFECSYPRSIGGILGKWSMLVLVGDPHREMRAISLNFLSSVRLRAVLLPEVER 174

Query: 168 -TIII----HSLTTFHDRHQ 182
            T+++       +TF  +HQ
Sbjct: 175 HTLLVLRAWPPSSTFSAQHQ 194

BLAST of Cmc11g0309801 vs. ExPASy Swiss-Prot
Match: O64989 (Cytochrome P450 90B1 OS=Arabidopsis thaliana OX=3702 GN=CYP90B1 PE=1 SV=2)

HSP 1 Score: 95.5 bits (236), Expect = 8.4e-19
Identity = 43/161 (26.71%), Postives = 89/161 (55.28%), Query Frame = 0

Query: 42  QTGYKFPPERRSWPLIGDSFDWYSAIASSHPSKYVEEQAKRYGKIFSCRVFGKCTVVSVD 101
           +T +  PP +  WP +G++  +     ++    ++++   +YGKI+   +FG+ T+VS D
Sbjct: 34  KTRFNLPPGKSGWPFLGETIGYLKPYTATTLGDFMQQHVSKYGKIYRSNLFGEPTIVSAD 93

Query: 102 PEFNRYVMQNEGILFQSSYPKSFRDLVGVNGVITAQGEQQRKLHGIASNFMRINKLNFSF 161
              NR+++QNEG LF+ SYP+S   ++G   ++   G+  R +  I+ NF+   +L    
Sbjct: 94  AGLNRFILQNEGRLFECSYPRSIGGILGKWSMLVLVGDMHRDMRSISLNFLSHARLRTIL 153

Query: 162 LKEIQTIIIHSLTTFHDRHQIISLQDACRKASMHTYIYYLL 203
           LK+++   +  L ++  ++ I S QD  +K + +    +++
Sbjct: 154 LKDVERHTLFVLDSW-QQNSIFSAQDEAKKFTFNLMAKHIM 193

BLAST of Cmc11g0309801 vs. ExPASy TrEMBL
Match: A0A1S4E208 (abietadienol/abietadienal oxidase isoform X1 OS=Cucumis melo OX=3656 GN=LOC103497741 PE=3 SV=1)

HSP 1 Score: 387.1 bits (993), Expect = 5.2e-104
Identity = 191/202 (94.55%), Postives = 196/202 (97.03%), Query Frame = 0

Query: 1   MTMWESSLVMWSFFIFTLLSATFLSNIFKNKKYNNNQNDQSQTGYKFPPERRSWPLIGDS 60
           MTMWESSLV+WSFFIFTLLSATFLSNIFKNKKYNNNQNDQSQTGYKFPP RRSWPLIGDS
Sbjct: 1   MTMWESSLVVWSFFIFTLLSATFLSNIFKNKKYNNNQNDQSQTGYKFPPGRRSWPLIGDS 60

Query: 61  FDWYSAIASSHPSKYVEEQAKRYGKIFSCRVFGKCTVVSVDPEFNRYVMQNEGILFQSSY 120
           FDWYSAIASSHPSKYVEEQAKRYGKIFSCRVFGKCTVVSVDPEFNRYVMQNEGILFQSSY
Sbjct: 61  FDWYSAIASSHPSKYVEEQAKRYGKIFSCRVFGKCTVVSVDPEFNRYVMQNEGILFQSSY 120

Query: 121 PKSFRDLVGVNGVITAQGEQQRKLHGIASNFMRINKLNFSFLKEIQTIIIHSLTTFHDRH 180
           PKSFRDLVGVNGVITAQGEQQRKLHGIASNFMRINKLNFSFLKEIQTIIIHSLTTFHDRH
Sbjct: 121 PKSFRDLVGVNGVITAQGEQQRKLHGIASNFMRINKLNFSFLKEIQTIIIHSLTTFHDRH 180

Query: 181 QIISLQDACRKASMHTYIYYLL 203
           QIISLQDACRK +++  +  LL
Sbjct: 181 QIISLQDACRKIAINVMVSQLL 202

BLAST of Cmc11g0309801 vs. ExPASy TrEMBL
Match: A0A0A0K7D5 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G001760 PE=3 SV=1)

HSP 1 Score: 350.1 bits (897), Expect = 7.0e-93
Identity = 175/203 (86.21%), Postives = 185/203 (91.13%), Query Frame = 0

Query: 2   TMWESSLVMWSFFIFTLLSATFLSNIFKNKKYN--NNQNDQSQTGYKFPPERRSWPLIGD 61
           TMWE SLV WSFFI TLL  TFL NI K KKYN  N+QNDQS+T YKFP  RRSWP+IGD
Sbjct: 15  TMWEPSLVAWSFFILTLLFTTFLPNILKKKKYNDQNDQNDQSRTRYKFPSGRRSWPVIGD 74

Query: 62  SFDWYSAIASSHPSKYVEEQAKRYGKIFSCRVFGKCTVVSVDPEFNRYVMQNEGILFQSS 121
           SF+WYSAIASSHPSKYVEEQAKRYGKIFSCRVFGKCTVVSVDP+FNRYVMQNEGILFQSS
Sbjct: 75  SFNWYSAIASSHPSKYVEEQAKRYGKIFSCRVFGKCTVVSVDPDFNRYVMQNEGILFQSS 134

Query: 122 YPKSFRDLVGVNGVITAQGEQQRKLHGIASNFMRINKLNFSFLKEIQTIIIHSLTTFHDR 181
           YPKSFRDLVGVNGVITAQGEQQRKLHGIASNFMRINKLNFSF+KEIQTIIIHSLTTFHDR
Sbjct: 135 YPKSFRDLVGVNGVITAQGEQQRKLHGIASNFMRINKLNFSFIKEIQTIIIHSLTTFHDR 194

Query: 182 HQIISLQDACRKASMHTYIYYLL 203
           HQIISLQDACRK +++  +  LL
Sbjct: 195 HQIISLQDACRKIAINLMVSQLL 217

BLAST of Cmc11g0309801 vs. ExPASy TrEMBL
Match: A0A6J1DWV6 (abietadienol/abietadienal oxidase isoform X1 OS=Momordica charantia OX=3673 GN=LOC111025171 PE=3 SV=1)

HSP 1 Score: 257.7 bits (657), Expect = 4.7e-65
Identity = 137/207 (66.18%), Postives = 157/207 (75.85%), Query Frame = 0

Query: 3   MWES-SLVMWSFF-IFTL-LSATFLSNIFKNKKYNNNQNDQSQTGYKFPPERRSWPLIGD 62
           MWE  + V+WS   I TL LS  FL+   KNKKY     D+ Q  Y+FPP RRSWP+IGD
Sbjct: 1   MWEQPASVLWSLLSILTLMLSILFLARTLKNKKYREG-GDEDQRAYRFPPGRRSWPVIGD 60

Query: 63  SFDWYSAIASSHPSKYVEEQAKRYGKIFSCRVFGKCTVVSVDPEFNRYVMQNEGILFQSS 122
           SF+WYSA+ASSHP KYVEEQ KRYGKIFSCRVFGK  VVS DP FNRYVMQNEG LFQSS
Sbjct: 61  SFNWYSAVASSHPPKYVEEQVKRYGKIFSCRVFGKWAVVSADPVFNRYVMQNEGKLFQSS 120

Query: 123 YPKSFRDLVGVNGVITAQGEQQRKLHGIASNFMRINK---LNFSFLKEIQTIIIHSLTTF 182
           YPKSFRDLVG+NGVIT QGEQQRKLH IA N MR++K   LNF FL+ IQTIIIH+L +F
Sbjct: 121 YPKSFRDLVGMNGVITVQGEQQRKLHAIACNMMRVHKHKVLNFPFLQHIQTIIIHTLCSF 180

Query: 183 -HDRHQIISLQDACRKASMHTYIYYLL 203
               HQ++ LQD CRK +++  +  LL
Sbjct: 181 SFPNHQVVYLQDVCRKVAINLMVTQLL 206

BLAST of Cmc11g0309801 vs. ExPASy TrEMBL
Match: A0A6J1DXX6 (abietadienol/abietadienal oxidase isoform X4 OS=Momordica charantia OX=3673 GN=LOC111025171 PE=3 SV=1)

HSP 1 Score: 257.7 bits (657), Expect = 4.7e-65
Identity = 137/207 (66.18%), Postives = 157/207 (75.85%), Query Frame = 0

Query: 3   MWES-SLVMWSFF-IFTL-LSATFLSNIFKNKKYNNNQNDQSQTGYKFPPERRSWPLIGD 62
           MWE  + V+WS   I TL LS  FL+   KNKKY     D+ Q  Y+FPP RRSWP+IGD
Sbjct: 1   MWEQPASVLWSLLSILTLMLSILFLARTLKNKKYREG-GDEDQRAYRFPPGRRSWPVIGD 60

Query: 63  SFDWYSAIASSHPSKYVEEQAKRYGKIFSCRVFGKCTVVSVDPEFNRYVMQNEGILFQSS 122
           SF+WYSA+ASSHP KYVEEQ KRYGKIFSCRVFGK  VVS DP FNRYVMQNEG LFQSS
Sbjct: 61  SFNWYSAVASSHPPKYVEEQVKRYGKIFSCRVFGKWAVVSADPVFNRYVMQNEGKLFQSS 120

Query: 123 YPKSFRDLVGVNGVITAQGEQQRKLHGIASNFMRINK---LNFSFLKEIQTIIIHSLTTF 182
           YPKSFRDLVG+NGVIT QGEQQRKLH IA N MR++K   LNF FL+ IQTIIIH+L +F
Sbjct: 121 YPKSFRDLVGMNGVITVQGEQQRKLHAIACNMMRVHKHKVLNFPFLQHIQTIIIHTLCSF 180

Query: 183 -HDRHQIISLQDACRKASMHTYIYYLL 203
               HQ++ LQD CRK +++  +  LL
Sbjct: 181 SFPNHQVVYLQDVCRKVAINLMVTQLL 206

BLAST of Cmc11g0309801 vs. ExPASy TrEMBL
Match: A0A6J1E1R6 (abietadienol/abietadienal oxidase isoform X3 OS=Momordica charantia OX=3673 GN=LOC111025171 PE=3 SV=1)

HSP 1 Score: 248.4 bits (633), Expect = 2.9e-62
Identity = 124/178 (69.66%), Postives = 141/178 (79.21%), Query Frame = 0

Query: 29  KNKKYNNNQNDQSQTGYKFPPERRSWPLIGDSFDWYSAIASSHPSKYVEEQAKRYGKIFS 88
           KNKKY     D+ Q  Y+FPP RRSWP+IGDSF+WYSA+ASSHP KYVEEQ KRYGKIFS
Sbjct: 19  KNKKYREG-GDEDQRAYRFPPGRRSWPVIGDSFNWYSAVASSHPPKYVEEQVKRYGKIFS 78

Query: 89  CRVFGKCTVVSVDPEFNRYVMQNEGILFQSSYPKSFRDLVGVNGVITAQGEQQRKLHGIA 148
           CRVFGK  VVS DP FNRYVMQNEG LFQSSYPKSFRDLVG+NGVIT QGEQQRKLH IA
Sbjct: 79  CRVFGKWAVVSADPVFNRYVMQNEGKLFQSSYPKSFRDLVGMNGVITVQGEQQRKLHAIA 138

Query: 149 SNFMRINK---LNFSFLKEIQTIIIHSLTTF-HDRHQIISLQDACRKASMHTYIYYLL 203
            N MR++K   LNF FL+ IQTIIIH+L +F    HQ++ LQD CRK +++  +  LL
Sbjct: 139 CNMMRVHKHKVLNFPFLQHIQTIIIHTLCSFSFPNHQVVYLQDVCRKVAINLMVTQLL 195

BLAST of Cmc11g0309801 vs. TAIR 10
Match: AT1G73340.1 (Cytochrome P450 superfamily protein )

HSP 1 Score: 193.0 bits (489), Expect = 2.8e-49
Identity = 89/158 (56.33%), Postives = 120/158 (75.95%), Query Frame = 0

Query: 45  YKFPPERRSWPLIGDSFDWYSAIASSHPSKYVEEQAKRYGKIFSCRVFGKCTVVSVDPEF 104
           ++ PP  R WPLIGD+F W +A+A SHPS +VE+Q K+YG+IFSC +FGK  VVS DP+F
Sbjct: 41  HRLPPGSRGWPLIGDTFAWLNAVAGSHPSSFVEKQIKKYGRIFSCSLFGKWAVVSADPDF 100

Query: 105 NRYVMQNEGILFQSSYPKSFRDLVGVNGVITAQGEQQRKLHGIASNFMRINKLNFSFLKE 164
           NR++MQNEG LFQSSYPKSFRDLVG +GVIT  G+QQR+LH IAS+ MR ++L   FL+ 
Sbjct: 101 NRFIMQNEGKLFQSSYPKSFRDLVGKDGVITVHGDQQRRLHSIASSMMRHDQLKTHFLEV 160

Query: 165 IQTIIIHSLTTFHDRHQIISLQDACRKASMHTYIYYLL 203
           I  +++ +L+ F D  +++ LQD CRK ++H  +  LL
Sbjct: 161 IPVVMLQTLSNFKD-GEVVLLQDICRKVAIHLMVNQLL 197

BLAST of Cmc11g0309801 vs. TAIR 10
Match: AT3G13730.1 (cytochrome P450, family 90, subfamily D, polypeptide 1 )

HSP 1 Score: 103.6 bits (257), Expect = 2.2e-22
Identity = 54/190 (28.42%), Postives = 100/190 (52.63%), Query Frame = 0

Query: 6   SSLVMWSFFIFTLLSA-----TFLSNIFKNKKYNNNQNDQSQTGYKFPPERRSWPLIGDS 65
           SSL+ +SFF F ++          S+    KK N++       G KFP     WP+IG++
Sbjct: 5   SSLLFFSFFFFIIIVIFNKINGLRSSPASKKKLNDHHVTSQSHGPKFPHGSLGWPVIGET 64

Query: 66  FDWYSAIASSHPSKYVEEQAKRYGKIFSCRVFGKCTVVSVDPEFNRYVMQNEGILFQSSY 125
            ++ S+  S  P  +++++   YG++F   +FG  T+VS D E NR V+Q++   F   Y
Sbjct: 65  IEFVSSAYSDRPESFMDKRRLMYGRVFKSHIFGTATIVSTDAEVNRAVLQSDSTAFVPFY 124

Query: 126 PKSFRDLVGVNGVITAQGEQQRKLHGIASNFMRINKLNFSFLKEIQTIIIHSLTTFHDRH 185
           PK+ R+L+G + ++   G   R+ HG+  +F++   L    ++++   +  S+  + +  
Sbjct: 125 PKTVRELMGKSSILLINGSLHRRFHGLVGSFLKSPLLKAQIVRDMHKFLSESMDLWSE-D 184

Query: 186 QIISLQDACR 191
           Q + LQD  +
Sbjct: 185 QPVLLQDVSK 193

BLAST of Cmc11g0309801 vs. TAIR 10
Match: AT3G50660.1 (Cytochrome P450 superfamily protein )

HSP 1 Score: 95.5 bits (236), Expect = 6.0e-20
Identity = 43/161 (26.71%), Postives = 89/161 (55.28%), Query Frame = 0

Query: 42  QTGYKFPPERRSWPLIGDSFDWYSAIASSHPSKYVEEQAKRYGKIFSCRVFGKCTVVSVD 101
           +T +  PP +  WP +G++  +     ++    ++++   +YGKI+   +FG+ T+VS D
Sbjct: 34  KTRFNLPPGKSGWPFLGETIGYLKPYTATTLGDFMQQHVSKYGKIYRSNLFGEPTIVSAD 93

Query: 102 PEFNRYVMQNEGILFQSSYPKSFRDLVGVNGVITAQGEQQRKLHGIASNFMRINKLNFSF 161
              NR+++QNEG LF+ SYP+S   ++G   ++   G+  R +  I+ NF+   +L    
Sbjct: 94  AGLNRFILQNEGRLFECSYPRSIGGILGKWSMLVLVGDMHRDMRSISLNFLSHARLRTIL 153

Query: 162 LKEIQTIIIHSLTTFHDRHQIISLQDACRKASMHTYIYYLL 203
           LK+++   +  L ++  ++ I S QD  +K + +    +++
Sbjct: 154 LKDVERHTLFVLDSW-QQNSIFSAQDEAKKFTFNLMAKHIM 193

BLAST of Cmc11g0309801 vs. TAIR 10
Match: AT4G36380.1 (Cytochrome P450 superfamily protein )

HSP 1 Score: 92.0 bits (227), Expect = 6.6e-19
Identity = 44/174 (25.29%), Postives = 93/174 (53.45%), Query Frame = 0

Query: 29  KNKKYNNNQNDQSQTGYKFPPERRSWPLIGDSFDWYSAIASSHPSKYVEEQAKRYGKIFS 88
           K K  +  ++++ +     P     WP+IG++ ++ +   SS P  +++++   YGK+F 
Sbjct: 51  KTKDGDEEEDNEEKKKGMIPNGSLGWPVIGETLNFIACGYSSRPVTFMDKRKSLYGKVFK 110

Query: 89  CRVFGKCTVVSVDPEFNRYVMQNEGILFQSSYPKSFRDLVGVNGVITAQGEQQRKLHGIA 148
             + G   ++S D E N+ V+QN G  F  +YPKS  +L+G N +++  G  Q++LH + 
Sbjct: 111 TNIIGTPIIISTDAEVNKVVLQNHGNTFVPAYPKSITELLGENSILSINGPHQKRLHTLI 170

Query: 149 SNFMRINKLNFSFLKEIQTIIIHSLTTFHDRHQIISLQDACRKASMHTYIYYLL 203
             F+R   L     ++I+  ++ +L ++  +  ++ +QD  +K +    +  L+
Sbjct: 171 GAFLRSPHLKDRITRDIEASVVLTLASW-AQLPLVHVQDEIKKMTFEILVKVLM 223

BLAST of Cmc11g0309801 vs. TAIR 10
Match: AT5G05690.1 (Cytochrome P450 superfamily protein )

HSP 1 Score: 92.0 bits (227), Expect = 6.6e-19
Identity = 49/185 (26.49%), Postives = 96/185 (51.89%), Query Frame = 0

Query: 48  PPERRSWPLIGDSFDWYSAIASSHPSKYVEEQAKRYGKIFSCRVFGKCTVVSVDPEFNRY 107
           PP     PLIG++F    A  + +P  +++E+  RYG +F   +FG+ T+ S DPE NR+
Sbjct: 32  PPGSLGLPLIGETFQLIGAYKTENPEPFIDERVARYGSVFMTHLFGEPTIFSADPETNRF 91

Query: 108 VMQNEGILFQSSYPKSFRDLVGVNGVITAQGEQQRKLHGIASNFMRINKLNFSFLKEIQT 167
           V+QNEG LF+ SYP S  +L+G + ++  +G   +++H +  +F   + +    + +I  
Sbjct: 92  VLQNEGKLFECSYPASICNLLGKHSLLLMKGSLHKRMHSLTMSFANSSIIKDHLMLDIDR 151

Query: 168 IIIHSLTTFHDRHQIISLQDACRKASMHTYIYYLLRYYEFNYVVLINNHLIFHVKMVFEL 227
           ++  +L ++  R   + L +  +K +    +  L+ +    +   +    +  ++  F L
Sbjct: 152 LVRFNLDSWSSR---VLLMEEAKKITFELTVKQLMSFDPGEWSESLRKEYLLVIEGFFSL 211

Query: 228 FLDNF 233
            L  F
Sbjct: 212 PLPLF 213

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_016902261.11.1e-10394.55PREDICTED: abietadienol/abietadienal oxidase isoform X1 [Cucumis melo][more]
XP_031742587.14.2e-9286.14abietadienol/abietadienal oxidase [Cucumis sativus] >XP_031742588.1 abietadienol... [more]
XP_038875678.14.4e-8178.00abietadienol/abietadienal oxidase [Benincasa hispida][more]
XP_022158708.19.8e-6566.18abietadienol/abietadienal oxidase isoform X1 [Momordica charantia][more]
XP_022158712.19.8e-6566.18abietadienol/abietadienal oxidase isoform X4 [Momordica charantia][more]
Match NameE-valueIdentityDescription
Q50EK69.6e-3136.52Abietadienol/abietadienal oxidase OS=Pinus taeda OX=3352 GN=CYP720B1 PE=1 SV=1[more]
Q50EK53.1e-2938.41Cytochrome P450 720B2 OS=Pinus taeda OX=3352 GN=CYP720B2 PE=2 SV=1[more]
Q94IA63.1e-2128.423-epi-6-deoxocathasterone 23-monooxygenase CYP90D1 OS=Arabidopsis thaliana OX=37... [more]
Q5CCK32.9e-1934.29Cytochrome P450 90B2 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP90B2 PE=1 SV... [more]
O649898.4e-1926.71Cytochrome P450 90B1 OS=Arabidopsis thaliana OX=3702 GN=CYP90B1 PE=1 SV=2[more]
Match NameE-valueIdentityDescription
A0A1S4E2085.2e-10494.55abietadienol/abietadienal oxidase isoform X1 OS=Cucumis melo OX=3656 GN=LOC10349... [more]
A0A0A0K7D57.0e-9386.21Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G001760 PE=3 SV=1[more]
A0A6J1DWV64.7e-6566.18abietadienol/abietadienal oxidase isoform X1 OS=Momordica charantia OX=3673 GN=L... [more]
A0A6J1DXX64.7e-6566.18abietadienol/abietadienal oxidase isoform X4 OS=Momordica charantia OX=3673 GN=L... [more]
A0A6J1E1R62.9e-6269.66abietadienol/abietadienal oxidase isoform X3 OS=Momordica charantia OX=3673 GN=L... [more]
Match NameE-valueIdentityDescription
AT1G73340.12.8e-4956.33Cytochrome P450 superfamily protein [more]
AT3G13730.12.2e-2228.42cytochrome P450, family 90, subfamily D, polypeptide 1 [more]
AT3G50660.16.0e-2026.71Cytochrome P450 superfamily protein [more]
AT4G36380.16.6e-1925.29Cytochrome P450 superfamily protein [more]
AT5G05690.16.6e-1926.49Cytochrome P450 superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Melon (Charmono) v1.1
Date Performed: 2022-10-13
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001128Cytochrome P450PFAMPF00067p450coord: 48..196
e-value: 4.4E-8
score: 32.3
IPR036396Cytochrome P450 superfamilyGENE3D1.10.630.10Cytochrome P450coord: 36..230
e-value: 6.7E-22
score: 79.9
IPR036396Cytochrome P450 superfamilySUPERFAMILY48264Cytochrome P450coord: 46..202
NoneNo IPR availablePANTHERPTHR24286:SF232CYTOCHROME P450 SUPERFAMILY PROTEINcoord: 32..198
NoneNo IPR availablePANTHERPTHR24286CYTOCHROME P450 26coord: 32..198

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cmc11g0309801.1Cmc11g0309801.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016132 brassinosteroid biosynthetic process
biological_process GO:0010268 brassinosteroid homeostasis
biological_process GO:0016125 sterol metabolic process
cellular_component GO:0016020 membrane
molecular_function GO:0020037 heme binding
molecular_function GO:0005506 iron ion binding
molecular_function GO:0004497 monooxygenase activity
molecular_function GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen