Cmc11g0294821 (gene) Melon (Charmono) v1.1

BLAST of Cmc11g0294821 vs. NCBI nr
Match: TYK13826.1 (putative polyprotein [Cucumis melo var. makuwa])

HSP 1 Score: 253.4 bits (646), Expect = 1.7e-63
Identity = 126/171 (73.68%), Postives = 147/171 (85.96%), Query Frame = 0

Query: 1    MSGCKAVSTPIVSHFRLSSSRCPVTEQERLKISNIPYCNVIGSIMYLMICTRLDLGYAIS 60
            MSG KAVSTP+ SHFRLSSS+CPVT+QER+++SNIPYCN +GSIMYLMICTR DLGYA+S
Sbjct: 918  MSGSKAVSTPLASHFRLSSSQCPVTKQERIEMSNIPYCNAVGSIMYLMICTRPDLGYAMS 977

Query: 61   MISRFMSNFGKEHWKAVKWVLRYLKGSASVSLCYSKDCDKSTLLEGFTNVDDAANLDKRR 120
            MISRFMSN GKEHWKAVKWVLRYLKGSASVSLCYS+DCDKSTLLEGFT+ D AA+LDKRR
Sbjct: 978  MISRFMSNPGKEHWKAVKWVLRYLKGSASVSLCYSRDCDKSTLLEGFTDADYAADLDKRR 1037

Query: 121  SLVAHIFRLYGNVVSWKVILQPVVLCQLLSQNIFLLVKKLRKQCGWKELLV 172
            SL  HIFRLYGNVVSWKV LQPVV     +++ ++ + +  K+  W + +V
Sbjct: 1038 SLSGHIFRLYGNVVSWKVNLQPVVALS-TTESEYISLGEAVKEAVWLKRIV 1087

BLAST of Cmc11g0294821 vs. NCBI nr
Match: KAA0047995.1 (retrotransposon protein, putative, Ty1-copia sub-class [Cucumis melo var. makuwa])

HSP 1 Score: 251.1 bits (640), Expect = 8.3e-63
Identity = 125/171 (73.10%), Postives = 146/171 (85.38%), Query Frame = 0

Query: 1    MSGCKAVSTPIVSHFRLSSSRCPVTEQERLKISNIPYCNVIGSIMYLMICTRLDLGYAIS 60
            MS  KAVSTP+ SHFRLSSS+CPVT+QER+++SNIPYCN +GSIMYLMICTR DLGYA+S
Sbjct: 1060 MSSSKAVSTPLASHFRLSSSQCPVTKQERIEMSNIPYCNAVGSIMYLMICTRPDLGYAMS 1119

Query: 61   MISRFMSNFGKEHWKAVKWVLRYLKGSASVSLCYSKDCDKSTLLEGFTNVDDAANLDKRR 120
            MISRFMSN GKEHWKAVKWVLRYLKGSASVSLCYS+DCDKSTLLEGFT+ D AA+LDKRR
Sbjct: 1120 MISRFMSNPGKEHWKAVKWVLRYLKGSASVSLCYSRDCDKSTLLEGFTDADYAADLDKRR 1179

Query: 121  SLVAHIFRLYGNVVSWKVILQPVVLCQLLSQNIFLLVKKLRKQCGWKELLV 172
            SL  HIFRLYGNVVSWKV LQPVV     +++ ++ + +  K+  W + +V
Sbjct: 1180 SLSGHIFRLYGNVVSWKVNLQPVVALS-TTESEYISLGEAVKEAVWLKRIV 1229

BLAST of Cmc11g0294821 vs. NCBI nr
Match: KAA0050719.1 (putative gag-pol polyprotein [Cucumis melo var. makuwa])

HSP 1 Score: 250.8 bits (639), Expect = 1.1e-62
Identity = 125/171 (73.10%), Postives = 146/171 (85.38%), Query Frame = 0

Query: 1    MSGCKAVSTPIVSHFRLSSSRCPVTEQERLKISNIPYCNVIGSIMYLMICTRLDLGYAIS 60
            MS  KAVSTP+ SHFRLSSS+CPVT+QER+++SNIPYCN +GSIMYLMICTR DLGYA+S
Sbjct: 1088 MSDSKAVSTPLASHFRLSSSQCPVTKQERIEMSNIPYCNAVGSIMYLMICTRPDLGYAMS 1147

Query: 61   MISRFMSNFGKEHWKAVKWVLRYLKGSASVSLCYSKDCDKSTLLEGFTNVDDAANLDKRR 120
            MISRFMSN GKEHWKAVKWVLRYLKGSASVSLCYS+DCDKSTLLEGFT+ D AA+LDKRR
Sbjct: 1148 MISRFMSNPGKEHWKAVKWVLRYLKGSASVSLCYSRDCDKSTLLEGFTDADYAADLDKRR 1207

Query: 121  SLVAHIFRLYGNVVSWKVILQPVVLCQLLSQNIFLLVKKLRKQCGWKELLV 172
            SL  HIFRLYGNVVSWKV LQPVV     +++ ++ + +  K+  W + +V
Sbjct: 1208 SLSGHIFRLYGNVVSWKVNLQPVVALS-TTESEYISLGEAVKEAVWLKRIV 1257

BLAST of Cmc11g0294821 vs. NCBI nr
Match: KAA0067463.1 (Retrovirus-related Pol polyprotein from transposon TNT 1-94 [Cucumis melo var. makuwa])

HSP 1 Score: 207.2 bits (526), Expect = 1.4e-49
Identity = 115/157 (73.25%), Postives = 120/157 (76.43%), Query Frame = 0

Query: 1   MSGCKAVSTPIVSHFRLSSSRCPVTEQERLKISNIPYCNVIGSIMYLMICTRLDLGYAIS 60
           MSGCKAVSTPIVSHFRLSSSRCPVTEQE                       RLDLGYAIS
Sbjct: 202 MSGCKAVSTPIVSHFRLSSSRCPVTEQE-----------------------RLDLGYAIS 261

Query: 61  MISRFMSNFGKEHWKAVKWVLRYLKGSASVSLCYSKDCDKSTLLEGFTNVDDAANLDKRR 120
           MISRFMSNFGKEHWKAVKWVLRYLKGSASVSLCYSKDCDKSTLLEGFTNVDDAANLDKRR
Sbjct: 262 MISRFMSNFGKEHWKAVKWVLRYLKGSASVSLCYSKDCDKSTLLEGFTNVDDAANLDKRR 321

Query: 121 SLVAHIFRLYGNVVSWKVILQPVVLCQLLSQNIFLLV 158
           SLVAHIFRLY   V   V L+ +V  + LSQ   L++
Sbjct: 322 SLVAHIFRLY--EVKEAVWLERIV-GEFLSQEFILII 332

BLAST of Cmc11g0294821 vs. NCBI nr
Match: KAA0067243.1 (retrotransposon protein, putative, Ty1-copia sub-class [Cucumis melo var. makuwa] >TYK10853.1 retrotransposon protein, putative, Ty1-copia sub-class [Cucumis melo var. makuwa])

HSP 1 Score: 189.5 bits (480), Expect = 2.9e-44
Identity = 97/141 (68.79%), Postives = 107/141 (75.89%), Query Frame = 0

Query: 3    GCKAVSTPIVSHFRLSSSRCPVTEQERLKISNIPYCNVIGSIMYLMICTRLDLGYAISMI 62
            G  A+STP+ SHFRLSSS+CPVT+QE                       R DLGYA+SMI
Sbjct: 899  GMDAISTPLASHFRLSSSQCPVTKQE-----------------------RPDLGYAMSMI 958

Query: 63   SRFMSNFGKEHWKAVKWVLRYLKGSASVSLCYSKDCDKSTLLEGFTNVDDAANLDKRRSL 122
            SRFMSN GKEHWKAVKWVLRYLKGSASVSLCYS+DCDKSTLLEGFT+ +  A+LDKRRSL
Sbjct: 959  SRFMSNPGKEHWKAVKWVLRYLKGSASVSLCYSRDCDKSTLLEGFTDANYVADLDKRRSL 1016

Query: 123  VAHIFRLYGNVVSWKVILQPV 144
              HIFRLYGNVVSWKV LQP+
Sbjct: 1019 SGHIFRLYGNVVSWKVNLQPI 1016

BLAST of Cmc11g0294821 vs. ExPASy Swiss-Prot
Match: P10978 (Retrovirus-related Pol polyprotein from transposon TNT 1-94 OS=Nicotiana tabacum OX=4097 PE=2 SV=1)

HSP 1 Score: 139.8 bits (351), Expect = 3.5e-32
Identity = 60/144 (41.67%), Postives = 93/144 (64.58%), Query Frame = 0

Query: 1    MSGCKAVSTPIVSHFRLSSSRCPVTEQERLKISNIPYCNVIGSIMYLMICTRLDLGYAIS 60
            M   K VSTP+  H +LS   CP T +E+  ++ +PY + +GS+MY M+CTR D+ +A+ 
Sbjct: 1075 MKNAKPVSTPLAGHLKLSKKMCPTTVEEKGNMAKVPYSSAVGSLMYAMVCTRPDIAHAVG 1134

Query: 61   MISRFMSNFGKEHWKAVKWVLRYLKGSASVSLCYSKDCDKSTLLEGFTNVDDAANLDKRR 120
            ++SRF+ N GKEHW+AVKW+LRYL+G+    LC+        +L+G+T+ D A ++D R+
Sbjct: 1135 VVSRFLENPGKEHWEAVKWILRYLRGTTGDCLCFG---GSDPILKGYTDADMAGDIDNRK 1194

Query: 121  SLVAHIFRLYGNVVSWKVILQPVV 145
            S   ++F   G  +SW+  LQ  V
Sbjct: 1195 SSTGYLFTFSGGAISWQSKLQKCV 1215

BLAST of Cmc11g0294821 vs. ExPASy Swiss-Prot
Match: P0CV72 (Secreted RxLR effector protein 161 OS=Plasmopara viticola OX=143451 GN=RXLR161 PE=2 SV=1)

HSP 1 Score: 84.0 bits (206), Expect = 2.3e-15
Identity = 40/106 (37.74%), Postives = 70/106 (66.04%), Query Frame = 0

Query: 32  ISNIPYCNVIGSIMYLMICTRLDLGYAISMISRFMSNFGKEHWKAVKWVLRYLKGSASVS 91
           + N+PY + +G+IMYLM+ TR DL  A+ ++S+F S+    HW+A+K VLRYL+ + +  
Sbjct: 1   MKNVPYLSAVGAIMYLMVVTRPDLAAAVGVLSQFASDPCPTHWQALKRVLRYLQSTQTYG 60

Query: 92  LCYSKDCDKSTLLEGFTNVDDAANLDKRRSLVAHIFRLYGNVVSWK 138
           L +++    +  L G+++ D A +++ RRS   ++F+L G  VSW+
Sbjct: 61  LEFTR--AGTAKLVGYSDADWAGDVESRRSTSGYLFKLNGGCVSWR 104

BLAST of Cmc11g0294821 vs. ExPASy Swiss-Prot
Match: P04146 (Copia protein OS=Drosophila melanogaster OX=7227 GN=GIP PE=1 SV=3)

HSP 1 Score: 81.6 bits (200), Expect = 1.1e-14
Identity = 54/175 (30.86%), Postives = 90/175 (51.43%), Query Frame = 0

Query: 1    MSGCKAVSTPIVS--HFRLSSSRCPVTEQERLKISNIPYCNVIGSIMYLMICTRLDLGYA 60
            M  C AVSTP+ S  ++ L +S          +  N P  ++IG +MY+M+CTR DL  A
Sbjct: 1152 MENCNAVSTPLPSKINYELLNSD---------EDCNTPCRSLIGCLMYIMLCTRPDLTTA 1211

Query: 61   ISMISRFMSNFGKEHWKAVKWVLRYLKGSASVSLCYSKDCDKSTLLEGFTNVDDAANLDK 120
            ++++SR+ S    E W+ +K VLRYLKG+  + L + K+      + G+ + D A +   
Sbjct: 1212 VNILSRYSSKNNSELWQNLKRVLRYLKGTIDMKLIFKKNLAFENKIIGYVDSDWAGSEID 1271

Query: 121  RRSLVAHIFRLYG-NVVSWKVILQPVVLCQLLSQNIFLLVKKLRKQCGWKELLVS 173
            R+S   ++F+++  N++ W    Q  V           L + +R+    K LL S
Sbjct: 1272 RKSTTGYLFKMFDFNLICWNTKRQNSVAASSTEAEYMALFEAVREALWLKFLLTS 1317

BLAST of Cmc11g0294821 vs. ExPASy Swiss-Prot
Match: Q9ZT94 (Retrovirus-related Pol polyprotein from transposon RE2 OS=Arabidopsis thaliana OX=3702 GN=RE2 PE=4 SV=1)

HSP 1 Score: 55.5 bits (132), Expect = 8.7e-07
Identity = 41/139 (29.50%), Postives = 68/139 (48.92%), Query Frame = 0

Query: 1    MSGCKAVSTPIVSHFRL---SSSRCPVTEQERLKISNIPYCNVIGSIMYLMICTRLDLGY 60
            M   K V+TP+ +  +L   S ++ P   +         Y  ++GS+ YL   TR DL Y
Sbjct: 1198 MLTAKPVATPMATSPKLTLHSGTKLPDPTE---------YRGIVGSLQYLAF-TRPDLSY 1257

Query: 61   AISMISRFMSNFGKEHWKAVKWVLRYLKGSASVSLCYSKDCDKSTLLEGFTNVDDAANLD 120
            A++ +S++M     +HW A+K VLRYL G+    +   K    +  L  +++ D A + D
Sbjct: 1258 AVNRLSQYMHMPTDDHWNALKRVLRYLAGTPDHGIFLKK--GNTLSLHAYSDADWAGDTD 1317

Query: 121  KRRSLVAHIFRLYGNVVSW 137
               S   +I  L  + +SW
Sbjct: 1318 DYVSTNGYIVYLGHHPISW 1324

BLAST of Cmc11g0294821 vs. ExPASy Swiss-Prot
Match: Q94HW2 (Retrovirus-related Pol polyprotein from transposon RE1 OS=Arabidopsis thaliana OX=3702 GN=RE1 PE=2 SV=1)

HSP 1 Score: 53.1 bits (126), Expect = 4.3e-06
Identity = 41/137 (29.93%), Postives = 69/137 (50.36%), Query Frame = 0

Query: 1    MSGCKAVSTPIVSHFRLS-SSRCPVTEQERLKISNIPYCNVIGSIMYLMICTRLDLGYAI 60
            M   K V+TP+    +LS  S   +T+          Y  ++GS+ YL   TR D+ YA+
Sbjct: 1215 MITAKPVTTPMAPSPKLSLYSGTKLTDPTE-------YRGIVGSLQYLAF-TRPDISYAV 1274

Query: 61   SMISRFMSNFGKEHWKAVKWVLRYLKGSASVSLCYSKDCDKSTLLEGFTNVDDAANLDKR 120
            + +S+FM    +EH +A+K +LRYL G+ +  +   K    +  L  +++ D A + D  
Sbjct: 1275 NRLSQFMHMPTEEHLQALKRILRYLAGTPNHGIFLKK--GNTLSLHAYSDADWAGDKDDY 1334

Query: 121  RSLVAHIFRLYGNVVSW 137
             S   +I  L  + +SW
Sbjct: 1335 VSTNGYIVYLGHHPISW 1341

BLAST of Cmc11g0294821 vs. ExPASy TrEMBL
Match: A0A5D3CTV2 (Putative polyprotein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold488G00910 PE=4 SV=1)

HSP 1 Score: 253.4 bits (646), Expect = 8.0e-64
Identity = 126/171 (73.68%), Postives = 147/171 (85.96%), Query Frame = 0

Query: 1    MSGCKAVSTPIVSHFRLSSSRCPVTEQERLKISNIPYCNVIGSIMYLMICTRLDLGYAIS 60
            MSG KAVSTP+ SHFRLSSS+CPVT+QER+++SNIPYCN +GSIMYLMICTR DLGYA+S
Sbjct: 918  MSGSKAVSTPLASHFRLSSSQCPVTKQERIEMSNIPYCNAVGSIMYLMICTRPDLGYAMS 977

Query: 61   MISRFMSNFGKEHWKAVKWVLRYLKGSASVSLCYSKDCDKSTLLEGFTNVDDAANLDKRR 120
            MISRFMSN GKEHWKAVKWVLRYLKGSASVSLCYS+DCDKSTLLEGFT+ D AA+LDKRR
Sbjct: 978  MISRFMSNPGKEHWKAVKWVLRYLKGSASVSLCYSRDCDKSTLLEGFTDADYAADLDKRR 1037

Query: 121  SLVAHIFRLYGNVVSWKVILQPVVLCQLLSQNIFLLVKKLRKQCGWKELLV 172
            SL  HIFRLYGNVVSWKV LQPVV     +++ ++ + +  K+  W + +V
Sbjct: 1038 SLSGHIFRLYGNVVSWKVNLQPVVALS-TTESEYISLGEAVKEAVWLKRIV 1087

BLAST of Cmc11g0294821 vs. ExPASy TrEMBL
Match: A0A5A7U2U7 (Retrotransposon protein, putative, Ty1-copia sub-class OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold385G00590 PE=4 SV=1)

HSP 1 Score: 251.1 bits (640), Expect = 4.0e-63
Identity = 125/171 (73.10%), Postives = 146/171 (85.38%), Query Frame = 0

Query: 1    MSGCKAVSTPIVSHFRLSSSRCPVTEQERLKISNIPYCNVIGSIMYLMICTRLDLGYAIS 60
            MS  KAVSTP+ SHFRLSSS+CPVT+QER+++SNIPYCN +GSIMYLMICTR DLGYA+S
Sbjct: 1060 MSSSKAVSTPLASHFRLSSSQCPVTKQERIEMSNIPYCNAVGSIMYLMICTRPDLGYAMS 1119

Query: 61   MISRFMSNFGKEHWKAVKWVLRYLKGSASVSLCYSKDCDKSTLLEGFTNVDDAANLDKRR 120
            MISRFMSN GKEHWKAVKWVLRYLKGSASVSLCYS+DCDKSTLLEGFT+ D AA+LDKRR
Sbjct: 1120 MISRFMSNPGKEHWKAVKWVLRYLKGSASVSLCYSRDCDKSTLLEGFTDADYAADLDKRR 1179

Query: 121  SLVAHIFRLYGNVVSWKVILQPVVLCQLLSQNIFLLVKKLRKQCGWKELLV 172
            SL  HIFRLYGNVVSWKV LQPVV     +++ ++ + +  K+  W + +V
Sbjct: 1180 SLSGHIFRLYGNVVSWKVNLQPVVALS-TTESEYISLGEAVKEAVWLKRIV 1229

BLAST of Cmc11g0294821 vs. ExPASy TrEMBL
Match: A0A5A7UB25 (Putative gag-pol polyprotein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold560G00190 PE=4 SV=1)

HSP 1 Score: 250.8 bits (639), Expect = 5.2e-63
Identity = 125/171 (73.10%), Postives = 146/171 (85.38%), Query Frame = 0

Query: 1    MSGCKAVSTPIVSHFRLSSSRCPVTEQERLKISNIPYCNVIGSIMYLMICTRLDLGYAIS 60
            MS  KAVSTP+ SHFRLSSS+CPVT+QER+++SNIPYCN +GSIMYLMICTR DLGYA+S
Sbjct: 1088 MSDSKAVSTPLASHFRLSSSQCPVTKQERIEMSNIPYCNAVGSIMYLMICTRPDLGYAMS 1147

Query: 61   MISRFMSNFGKEHWKAVKWVLRYLKGSASVSLCYSKDCDKSTLLEGFTNVDDAANLDKRR 120
            MISRFMSN GKEHWKAVKWVLRYLKGSASVSLCYS+DCDKSTLLEGFT+ D AA+LDKRR
Sbjct: 1148 MISRFMSNPGKEHWKAVKWVLRYLKGSASVSLCYSRDCDKSTLLEGFTDADYAADLDKRR 1207

Query: 121  SLVAHIFRLYGNVVSWKVILQPVVLCQLLSQNIFLLVKKLRKQCGWKELLV 172
            SL  HIFRLYGNVVSWKV LQPVV     +++ ++ + +  K+  W + +V
Sbjct: 1208 SLSGHIFRLYGNVVSWKVNLQPVVALS-TTESEYISLGEAVKEAVWLKRIV 1257

BLAST of Cmc11g0294821 vs. ExPASy TrEMBL
Match: A0A5A7VH96 (Retrovirus-related Pol polyprotein from transposon TNT 1-94 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold40G001340 PE=4 SV=1)

HSP 1 Score: 207.2 bits (526), Expect = 6.6e-50
Identity = 115/157 (73.25%), Postives = 120/157 (76.43%), Query Frame = 0

Query: 1   MSGCKAVSTPIVSHFRLSSSRCPVTEQERLKISNIPYCNVIGSIMYLMICTRLDLGYAIS 60
           MSGCKAVSTPIVSHFRLSSSRCPVTEQE                       RLDLGYAIS
Sbjct: 202 MSGCKAVSTPIVSHFRLSSSRCPVTEQE-----------------------RLDLGYAIS 261

Query: 61  MISRFMSNFGKEHWKAVKWVLRYLKGSASVSLCYSKDCDKSTLLEGFTNVDDAANLDKRR 120
           MISRFMSNFGKEHWKAVKWVLRYLKGSASVSLCYSKDCDKSTLLEGFTNVDDAANLDKRR
Sbjct: 262 MISRFMSNFGKEHWKAVKWVLRYLKGSASVSLCYSKDCDKSTLLEGFTNVDDAANLDKRR 321

Query: 121 SLVAHIFRLYGNVVSWKVILQPVVLCQLLSQNIFLLV 158
           SLVAHIFRLY   V   V L+ +V  + LSQ   L++
Sbjct: 322 SLVAHIFRLY--EVKEAVWLERIV-GEFLSQEFILII 332

BLAST of Cmc11g0294821 vs. ExPASy TrEMBL
Match: A0A5A7VGM4 (Retrotransposon protein, putative, Ty1-copia sub-class OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold231G00240 PE=4 SV=1)

HSP 1 Score: 189.5 bits (480), Expect = 1.4e-44
Identity = 97/141 (68.79%), Postives = 107/141 (75.89%), Query Frame = 0

Query: 3    GCKAVSTPIVSHFRLSSSRCPVTEQERLKISNIPYCNVIGSIMYLMICTRLDLGYAISMI 62
            G  A+STP+ SHFRLSSS+CPVT+QE                       R DLGYA+SMI
Sbjct: 899  GMDAISTPLASHFRLSSSQCPVTKQE-----------------------RPDLGYAMSMI 958

Query: 63   SRFMSNFGKEHWKAVKWVLRYLKGSASVSLCYSKDCDKSTLLEGFTNVDDAANLDKRRSL 122
            SRFMSN GKEHWKAVKWVLRYLKGSASVSLCYS+DCDKSTLLEGFT+ +  A+LDKRRSL
Sbjct: 959  SRFMSNPGKEHWKAVKWVLRYLKGSASVSLCYSRDCDKSTLLEGFTDANYVADLDKRRSL 1016

Query: 123  VAHIFRLYGNVVSWKVILQPV 144
              HIFRLYGNVVSWKV LQP+
Sbjct: 1019 SGHIFRLYGNVVSWKVNLQPI 1016

BLAST of Cmc11g0294821 vs. TAIR 10
Match: AT4G23160.1 (cysteine-rich RLK (RECEPTOR-like protein kinase) 8 )

HSP 1 Score: 57.4 bits (137), Expect = 1.6e-08
Identity = 37/108 (34.26%), Postives = 58/108 (53.70%), Query Frame = 0

Query: 37  YCNVIGSIMYLMICTRLDLGYAISMISRFMSNFGKEHWKAVKWVLRYLKGSASVSLCYSK 96
           Y  +IG +MYL I TRLD+ +A++ +S+F       H +AV  +L Y+KG+    L YS 
Sbjct: 378 YRRLIGRLMYLQI-TRLDISFAVNKLSQFSEAPRLAHQQAVMKILHYIKGTVGQGLFYSS 437

Query: 97  DCDKSTLLEGFTNVDDAANLDKRRSLVAHIFRLYGNVVSWKVILQPVV 145
             +    L+ F++    +  D RRS   +   L  +++SWK   Q VV
Sbjct: 438 QAEMQ--LQVFSDASFQSCKDTRRSTNGYCMFLGTSLISWKSKKQQVV 482