Cmc11g0293371 (gene) Melon (Charmono) v1.1

Overview
NameCmc11g0293371
Typegene
OrganismCucumis melo L. var. cantalupensis cv. Charmono (Melon (Charmono) v1.1)
DescriptionCyclin
LocationCMiso1.1chr11: 9600954 .. 9601954 (+)
RNA-Seq ExpressionCmc11g0293371
SyntenyCmc11g0293371
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GTACACACCCAAATTTCTCCAACAATGTTAACAGGCAACGACTTCGCAGCTCCGGGGCGAGCCGAGGGCGATAATGCAGCCGAGCCAACCCCAAGAGTACTCATCATATTAGCCTTCGTCCTTGACCGGCTGGTGGCTCGTAACGACCGCCTCTTAAATGAGCTAACCCAACAGCTTGAAGAGTTGGGCTGCTGCAGCTCTTCCTCTAACCATTTAGGAAACAGCTTCAATGCGTTCCATGGAGTCCGAGCCCCAACCATCTCTATATTGAAGTACTTGGAGAGAATTTACAAATACACCAATTGTAGCCCTTCTTGTCTCGTGGTTGGATTTGTATACATAGATAGATTGATCCATCGACACCCCGACTCCCTTGTCATCTCCTTGAATGTTCATCGCCTTCTCGTCACAAGTGTCATGGTTGCATCTAAGATGCTTGACGACGTGTGAGAGTCTTCAACTCATACCTCTTCTTGGTCTAATTTATTTTGTTTTTGGAGAGTCTATTTGGAATGACATTTTGTTAAAATGATTGACAGGCACTATAACAACGCATTTTATGCCCGAGTGGGCGGGGTGAGCAAAGGAGAGTTGAATAAGCTGGAATTAGAGATGCTTTTTTTGTTGGATTTTGGAGTGACAGTTAGTTCGAGGGCTTTTGAAACTTATTGTTGCCATTTGGAGAAAGAGATGTTGTTGAATGGCAATGGGGAGATGCAAAGAGTAGAAAGACCCTTGGTTATGCCTACTAATACTTTGGATGACGTGTTGGAAATTTCTGTGGACGACACTCTTCTTGTTTCTTCTTCACCACCTTAATTAGTTAAAATCTTCCTCATTTCATTATTTTTTCTTCTCTTCTCTCCTTATTAGATATATATATTCTTGTATATACGATGTTTTGTTTCTTATTCAACAATGGAGAGAATTGAGGCTCTATGCTTGAAAAGAAAGAGTGGATCTTGGGAAAGCTTAGAGAATGACAAAGACTAAAAAAACAT

mRNA sequence

GTACACACCCAAATTTCTCCAACAATGTTAACAGGCAACGACTTCGCAGCTCCGGGGCGAGCCGAGGGCGATAATGCAGCCGAGCCAACCCCAAGAGTACTCATCATATTAGCCTTCGTCCTTGACCGGCTGGTGGCTCGTAACGACCGCCTCTTAAATGAGCTAACCCAACAGCTTGAAGAGTTGGGCTGCTGCAGCTCTTCCTCTAACCATTTAGGAAACAGCTTCAATGCGTTCCATGGAGTCCGAGCCCCAACCATCTCTATATTGAAGTACTTGGAGAGAATTTACAAATACACCAATTGTAGCCCTTCTTGTCTCGTGGTTGGATTTGTATACATAGATAGATTGATCCATCGACACCCCGACTCCCTTGTCATCTCCTTGAATGTTCATCGCCTTCTCGTCACAAGTGTCATGGTTGCATCTAAGATGCTTGACGACGTGCACTATAACAACGCATTTTATGCCCGAGTGGGCGGGGTGAGCAAAGGAGAGTTGAATAAGCTGGAATTAGAGATGCTTTTTTTGTTGGATTTTGGAGTGACAGTTAGTTCGAGGGCTTTTGAAACTTATTGTTGCCATTTGGAGAAAGAGATGTTGTTGAATGGCAATGGGGAGATGCAAAGAGTAGAAAGACCCTTGGTTATGCCTACTAATACTTTGGATGACGTGTTGGAAATTTCTGTGGACGACACTCTTCTTGTTTCTTCTTCACCACCTTAATTAGTTAAAATCTTCCTCATTTCATTATTTTTTCTTCTCTTCTCTCCTTATTAGATATATATATTCTTGTATATACGATGTTTTGTTTCTTATTCAACAATGGAGAGAATTGAGGCTCTATGCTTGAAAAGAAAGAGTGGATCTTGGGAAAGCTTAGAGAATGACAAAGACTAAAAAAACAT

Coding sequence (CDS)

ATGTTAACAGGCAACGACTTCGCAGCTCCGGGGCGAGCCGAGGGCGATAATGCAGCCGAGCCAACCCCAAGAGTACTCATCATATTAGCCTTCGTCCTTGACCGGCTGGTGGCTCGTAACGACCGCCTCTTAAATGAGCTAACCCAACAGCTTGAAGAGTTGGGCTGCTGCAGCTCTTCCTCTAACCATTTAGGAAACAGCTTCAATGCGTTCCATGGAGTCCGAGCCCCAACCATCTCTATATTGAAGTACTTGGAGAGAATTTACAAATACACCAATTGTAGCCCTTCTTGTCTCGTGGTTGGATTTGTATACATAGATAGATTGATCCATCGACACCCCGACTCCCTTGTCATCTCCTTGAATGTTCATCGCCTTCTCGTCACAAGTGTCATGGTTGCATCTAAGATGCTTGACGACGTGCACTATAACAACGCATTTTATGCCCGAGTGGGCGGGGTGAGCAAAGGAGAGTTGAATAAGCTGGAATTAGAGATGCTTTTTTTGTTGGATTTTGGAGTGACAGTTAGTTCGAGGGCTTTTGAAACTTATTGTTGCCATTTGGAGAAAGAGATGTTGTTGAATGGCAATGGGGAGATGCAAAGAGTAGAAAGACCCTTGGTTATGCCTACTAATACTTTGGATGACGTGTTGGAAATTTCTGTGGACGACACTCTTCTTGTTTCTTCTTCACCACCTTAA

Protein sequence

MLTGNDFAAPGRAEGDNAAEPTPRVLIILAFVLDRLVARNDRLLNELTQQLEELGCCSSSSNHLGNSFNAFHGVRAPTISILKYLERIYKYTNCSPSCLVVGFVYIDRLIHRHPDSLVISLNVHRLLVTSVMVASKMLDDVHYNNAFYARVGGVSKGELNKLELEMLFLLDFGVTVSSRAFETYCCHLEKEMLLNGNGEMQRVERPLVMPTNTLDDVLEISVDDTLLVSSSPP
Homology
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BLAST of Cmc11g0293371 vs. NCBI nr
Match: XP_008455834.1 (PREDICTED: cyclin-U1-1 [Cucumis melo] >KAA0042430.1 cyclin-U1-1 [Cucumis melo var. makuwa] >TYJ98312.1 cyclin-U1-1 [Cucumis melo var. makuwa])

HSP 1 Score: 465.7 bits (1197), Expect = 2.4e-127
Identity = 233/233 (100.00%), Postives = 233/233 (100.00%), Query Frame = 0 

Query: 1   MLTGNDFAAPGRAEGDNAAEPTPRVLIILAFVLDRLVARNDRLLNELTQQLEELGCCSSS 60
           MLTGNDFAAPGRAEGDNAAEPTPRVLIILAFVLDRLVARNDRLLNELTQQLEELGCCSSS
Sbjct: 1   MLTGNDFAAPGRAEGDNAAEPTPRVLIILAFVLDRLVARNDRLLNELTQQLEELGCCSSS 60

Query: 61  SNHLGNSFNAFHGVRAPTISILKYLERIYKYTNCSPSCLVVGFVYIDRLIHRHPDSLVIS 120
           SNHLGNSFNAFHGVRAPTISILKYLERIYKYTNCSPSCLVVGFVYIDRLIHRHPDSLVIS
Sbjct: 61  SNHLGNSFNAFHGVRAPTISILKYLERIYKYTNCSPSCLVVGFVYIDRLIHRHPDSLVIS 120

Query: 121 LNVHRLLVTSVMVASKMLDDVHYNNAFYARVGGVSKGELNKLELEMLFLLDFGVTVSSRA 180
           LNVHRLLVTSVMVASKMLDDVHYNNAFYARVGGVSKGELNKLELEMLFLLDFGVTVSSRA
Sbjct: 121 LNVHRLLVTSVMVASKMLDDVHYNNAFYARVGGVSKGELNKLELEMLFLLDFGVTVSSRA 180

Query: 181 FETYCCHLEKEMLLNGNGEMQRVERPLVMPTNTLDDVLEISVDDTLLVSSSPP 234
           FETYCCHLEKEMLLNGNGEMQRVERPLVMPTNTLDDVLEISVDDTLLVSSSPP
Sbjct: 181 FETYCCHLEKEMLLNGNGEMQRVERPLVMPTNTLDDVLEISVDDTLLVSSSPP 233

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BLAST of Cmc11g0293371 vs. NCBI nr
Match: KAE8652302.1 (hypothetical protein Csa_022481 [Cucumis sativus])

HSP 1 Score: 452.2 bits (1162), Expect = 2.7e-123
Identity = 226/233 (97.00%), Postives = 229/233 (98.28%), Query Frame = 0 

Query: 1   MLTGNDFAAPGRAEGDNAAEPTPRVLIILAFVLDRLVARNDRLLNELTQQLEELGCCSSS 60
           MLTG+DFAAPGR EGDNAAEPTPRVLIILAFVLDRLVARNDRLLNEL+QQLEELGCCSSS
Sbjct: 65  MLTGSDFAAPGRVEGDNAAEPTPRVLIILAFVLDRLVARNDRLLNELSQQLEELGCCSSS 124

Query: 61  SNHLGNSFNAFHGVRAPTISILKYLERIYKYTNCSPSCLVVGFVYIDRLIHRHPDSLVIS 120
           SNHLGNSFNAFHGVRAPTISILKYLERIYKYTNCSPSCLVVGFVYIDRLIHRHPDSLVIS
Sbjct: 125 SNHLGNSFNAFHGVRAPTISILKYLERIYKYTNCSPSCLVVGFVYIDRLIHRHPDSLVIS 184

Query: 121 LNVHRLLVTSVMVASKMLDDVHYNNAFYARVGGVSKGELNKLELEMLFLLDFGVTVSSRA 180
           LNVHRLLVTSVMVASKMLDDVHYNNAFYARVGGVSKGELNKLELEMLFLLDFGVTVSSR 
Sbjct: 185 LNVHRLLVTSVMVASKMLDDVHYNNAFYARVGGVSKGELNKLELEMLFLLDFGVTVSSRV 244

Query: 181 FETYCCHLEKEMLLNGNGEMQRVERPLVMPTNTLDDVLEISVDDTLLVSSSPP 234
           FETYC HLEKEMLLNGNGEMQRVERPL+MPTNTLDDV EISVDDTLLVSSSPP
Sbjct: 245 FETYCWHLEKEMLLNGNGEMQRVERPLIMPTNTLDDVSEISVDDTLLVSSSPP 297

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BLAST of Cmc11g0293371 vs. NCBI nr
Match: XP_004151930.2 (cyclin-U1-1 [Cucumis sativus])

HSP 1 Score: 452.2 bits (1162), Expect = 2.7e-123
Identity = 226/233 (97.00%), Postives = 229/233 (98.28%), Query Frame = 0 

Query: 1   MLTGNDFAAPGRAEGDNAAEPTPRVLIILAFVLDRLVARNDRLLNELTQQLEELGCCSSS 60
           MLTG+DFAAPGR EGDNAAEPTPRVLIILAFVLDRLVARNDRLLNEL+QQLEELGCCSSS
Sbjct: 57  MLTGSDFAAPGRVEGDNAAEPTPRVLIILAFVLDRLVARNDRLLNELSQQLEELGCCSSS 116

Query: 61  SNHLGNSFNAFHGVRAPTISILKYLERIYKYTNCSPSCLVVGFVYIDRLIHRHPDSLVIS 120
           SNHLGNSFNAFHGVRAPTISILKYLERIYKYTNCSPSCLVVGFVYIDRLIHRHPDSLVIS
Sbjct: 117 SNHLGNSFNAFHGVRAPTISILKYLERIYKYTNCSPSCLVVGFVYIDRLIHRHPDSLVIS 176

Query: 121 LNVHRLLVTSVMVASKMLDDVHYNNAFYARVGGVSKGELNKLELEMLFLLDFGVTVSSRA 180
           LNVHRLLVTSVMVASKMLDDVHYNNAFYARVGGVSKGELNKLELEMLFLLDFGVTVSSR 
Sbjct: 177 LNVHRLLVTSVMVASKMLDDVHYNNAFYARVGGVSKGELNKLELEMLFLLDFGVTVSSRV 236

Query: 181 FETYCCHLEKEMLLNGNGEMQRVERPLVMPTNTLDDVLEISVDDTLLVSSSPP 234
           FETYC HLEKEMLLNGNGEMQRVERPL+MPTNTLDDV EISVDDTLLVSSSPP
Sbjct: 237 FETYCWHLEKEMLLNGNGEMQRVERPLIMPTNTLDDVSEISVDDTLLVSSSPP 289

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BLAST of Cmc11g0293371 vs. NCBI nr
Match: XP_038901956.1 (cyclin-U1-1 [Benincasa hispida])

HSP 1 Score: 420.2 bits (1079), Expect = 1.1e-113
Identity = 216/235 (91.91%), Postives = 223/235 (94.89%), Query Frame = 0 

Query: 1   MLTGNDFAAPGRAEGDNAAEPTPRVLIILAFVLDRLVARNDRLLNELTQ--QLEELGCCS 60
           MLTG+DFAAPGRAEGDNAAEPTPRVLIILAFVLDRLVARNDRLLN ++Q  QLEE GCCS
Sbjct: 1   MLTGSDFAAPGRAEGDNAAEPTPRVLIILAFVLDRLVARNDRLLNGVSQSRQLEESGCCS 60

Query: 61  SSSNHLGNSFNAFHGVRAPTISILKYLERIYKYTNCSPSCLVVGFVYIDRLIHRHPDSLV 120
            SSNHLGNSFNAFHGVRAPTISILKYLERIYKYTNCSPSCLVVGFVYIDRLIHRHPDSL+
Sbjct: 61  PSSNHLGNSFNAFHGVRAPTISILKYLERIYKYTNCSPSCLVVGFVYIDRLIHRHPDSLI 120

Query: 121 ISLNVHRLLVTSVMVASKMLDDVHYNNAFYARVGGVSKGELNKLELEMLFLLDFGVTVSS 180
           ISLNVHRLLVTS+MVASKMLDDVHYNNAFYARVGGVSK ELNKLELEMLFLLDFGVTVSS
Sbjct: 121 ISLNVHRLLVTSIMVASKMLDDVHYNNAFYARVGGVSKVELNKLELEMLFLLDFGVTVSS 180

Query: 181 RAFETYCCHLEKEMLLNGNGEMQRVERPLVMPTNTLDDVLEISVDDTLLVSSSPP 234
           R FETYC HLEKEMLLNGNGEMQR+ERPL MPTN+LDDV EISVDDT L SSSPP
Sbjct: 181 RVFETYCWHLEKEMLLNGNGEMQRMERPL-MPTNSLDDVSEISVDDT-LASSSPP 233

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BLAST of Cmc11g0293371 vs. NCBI nr
Match: KAG6604845.1 (Cyclin-U1-1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 387.1 bits (993), Expect = 1.1e-103
Identity = 198/233 (84.98%), Postives = 213/233 (91.42%), Query Frame = 0 

Query: 1   MLTGNDFAAPGRAEGDNAAEPTPRVLIILAFVLDRLVARNDRLLNELTQQLEELGCCSSS 60
           MLTGNDF A G AEGDN AEPTPRVLIILA VLDRLVARNDR+++ +T Q+EE   CS+S
Sbjct: 1   MLTGNDFPANGLAEGDNVAEPTPRVLIILACVLDRLVARNDRIVDGITHQIEESSSCSTS 60

Query: 61  SNHLGNSFNAFHGVRAPTISILKYLERIYKYTNCSPSCLVVGFVYIDRLIHRHPDSLVIS 120
             H+GNSFNAFHGVRAP ISILKYLERIYKYTNCSPSCLVVGFVYIDRLIHRHP+SLVIS
Sbjct: 61  --HIGNSFNAFHGVRAPAISILKYLERIYKYTNCSPSCLVVGFVYIDRLIHRHPNSLVIS 120

Query: 121 LNVHRLLVTSVMVASKMLDDVHYNNAFYARVGGVSKGELNKLELEMLFLLDFGVTVSSRA 180
           LNVHRLLVTSVMVASK+LDDVHYNNAFYARVGGV+K ELNKLELE+LFLLDFGVTVSSRA
Sbjct: 121 LNVHRLLVTSVMVASKILDDVHYNNAFYARVGGVNKAELNKLELELLFLLDFGVTVSSRA 180

Query: 181 FETYCCHLEKEMLLNGNGEMQRVERPLVMPTNTLDDVLEISVDDTLLVSSSPP 234
           FETYC HLEKEMLLNGNGE+QR+ERP +M TN+LDDV EISVDDT L SSSPP
Sbjct: 181 FETYCLHLEKEMLLNGNGELQRIERP-IMATNSLDDVSEISVDDT-LASSSPP 229

[Close]
BLAST of Cmc11g0293371 vs. ExPASy Swiss-Prot
Match: Q9LJ45 (Cyclin-U1-1 OS=Arabidopsis thaliana OX=3702 GN=CYCU1-1 PE=1 SV=1)

HSP 1 Score: 232.6 bits (592), Expect = 4.4e-60
Identity = 125/222 (56.31%), Postives = 152/222 (68.47%), Query Frame = 0 

Query: 1   MLT--GNDFAAPGRAEGDNAAEPTPRVLIILAFVLDRLVARNDRLLNELTQQLEELGCCS 60
           MLT  G+D   P           TPRVL I++ V+++LVARN+ L              +
Sbjct: 1   MLTAAGDDELDPVVGPESATEAATPRVLTIISHVMEKLVARNEWL--------------A 60

Query: 61  SSSNHLGNSFNAFHGVRAPTISILKYLERIYKYTNCSPSCLVVGFVYIDRLIHRHPDSLV 120
             +   G S  AFHGVRAP+ISI KYLERIYKYT CSP+C VVG+VYIDRL H+HP SLV
Sbjct: 61  KQTKGFGKSLEAFHGVRAPSISIAKYLERIYKYTKCSPACFVVGYVYIDRLAHKHPGSLV 120

Query: 121 ISLNVHRLLVTSVMVASKMLDDVHYNNAFYARVGGVSKGELNKLELEMLFLLDFGVTVSS 180
           +SLNVHRLLVT VM+A+K+LDDVHYNN FYARVGGVS  +LNK+ELE+LFLLDF VTVS 
Sbjct: 121 VSLNVHRLLVTCVMIAAKILDDVHYNNEFYARVGGVSNADLNKMELELLFLLDFRVTVSF 180

Query: 181 RAFETYCCHLEKEMLLNGNGEMQRVERPL---VMPTNTLDDV 218
           R FE+YC HLEKEM LN      +  +P+   + P +TL  +
Sbjct: 181 RVFESYCFHLEKEMQLNDAVSSLKDIQPMQESLSPASTLSSL 208

[Close]
BLAST of Cmc11g0293371 vs. ExPASy Swiss-Prot
Match: Q75HV0 (Cyclin-P3-1 OS=Oryza sativa subsp. japonica OX=39947 GN=CYCP3-1 PE=3 SV=1)

HSP 1 Score: 158.7 bits (400), Expect = 8.1e-38
Identity = 82/172 (47.67%), Postives = 114/172 (66.28%), Query Frame = 0 

Query: 23  PRVLIILAFVLDRLVARNDRLLNELTQQLEELGCCSSSSNHLGNSFNAFHGVRAPTISIL 82
           P+VL++LA  LDR V +N+ LL+               SN + +S   FHG RAP +SI 
Sbjct: 36  PKVLLLLAAYLDRSVQKNEDLLD---------------SNKIKDSSTIFHGHRAPDLSIK 95

Query: 83  KYLERIYKYTNCSPSCLVVGFVYIDRLIHRHPDSLVISLNVHRLLVTSVMVASKMLDDVH 142
            Y ERI+KY+ CSPSC V+  +Y++R + + P   + SL+VHRLL+TSV+VA+K  DD  
Sbjct: 96  LYAERIFKYSECSPSCFVLALIYMERYL-QQPHVYMTSLSVHRLLITSVVVAAKFTDDAF 155

Query: 143 YNNAFYARVGGVSKGELNKLELEMLFLLDFGVTVSSRAFETYCCHLEKEMLL 195
           +NNAFYARVGG+S  E+N+LEL++LF LDF + V    F +YC  LEKE ++
Sbjct: 156 FNNAFYARVGGISTVEMNRLELDLLFNLDFRLKVDLETFGSYCLQLEKETMV 191

[Close]
BLAST of Cmc11g0293371 vs. ExPASy Swiss-Prot
Match: O80513 (Cyclin-U4-1 OS=Arabidopsis thaliana OX=3702 GN=CYCU4-1 PE=1 SV=1)

HSP 1 Score: 158.7 bits (400), Expect = 8.1e-38
Identity = 80/171 (46.78%), Postives = 107/171 (62.57%), Query Frame = 0 

Query: 24  RVLIILAFVLDRLVARNDRLLNELTQQLEELGCCSSSSNHLGNSFNAFHGVRAPTISILK 83
           +++  L+ +L+R+   ND      TQ                   + FHG+  PTI+I  
Sbjct: 12  KLIAFLSSLLERVAESNDLTRRVATQ---------------SQRVSVFHGLSRPTITIQS 71

Query: 84  YLERIYKYTNCSPSCLVVGFVYIDRLIHRHPDSLVISLNVHRLLVTSVMVASKMLDDVHY 143
           YLERI+KY NCSPSC VV +VY+DR  HR P   + S NVHRLL+TSVMVA+K LDD++Y
Sbjct: 72  YLERIFKYANCSPSCFVVAYVYLDRFTHRQPSLPINSFNVHRLLITSVMVAAKFLDDLYY 131

Query: 144 NNAFYARVGGVSKGELNKLELEMLFLLDFGVTVSSRAFETYCCHLEKEMLL 195
           NNA+YA+VGG+S  E+N LEL+ LF L F + V+   F  Y  +L+KEM L
Sbjct: 132 NNAYYAKVGGISTKEMNFLELDFLFGLGFELNVTPNTFNAYFSYLQKEMTL 167

[Close]
BLAST of Cmc11g0293371 vs. ExPASy Swiss-Prot
Match: Q0J9W0 (Cyclin-P1-1 OS=Oryza sativa subsp. japonica OX=39947 GN=CYCP1-1 PE=3 SV=2)

HSP 1 Score: 157.5 bits (397), Expect = 1.8e-37
Identity = 85/184 (46.20%), Postives = 114/184 (61.96%), Query Frame = 0 

Query: 18  AAEPTPRVLIILAFVLDRLVARNDRLLNELTQQLEELGCCSSSSNHLG---NSFNAFHGV 77
           A+ P P  L ++A  + RLVARND         +E L     ++  LG    +F A  G 
Sbjct: 18  ASAPPPPELDMVARAVQRLVARND--------AVEALSGGGEAAAGLGAGMAAFEAARGA 77

Query: 78  RAPTISILKYLERIYKYTNCSPSCLVVGFVYIDRLIHRHPDSLVISLNVHRLLVTSVMVA 137
            AP I + +YLER+++Y    P C VV + Y+D   HR P + V S NVHRLL+  ++VA
Sbjct: 78  PAPRIGVAQYLERVHRYAGLEPECYVVAYAYVDMAAHRRPAAAVASRNVHRLLLACLLVA 137

Query: 138 SKMLDDVHYNNAFYARVGGVSKGELNKLELEMLFLLDFGVTVSSRAFETYCCHLEKEMLL 197
           SK+LDD H+NNAF+ARVGGVS  E+N+LELE+L +LDF V +S R +E Y  HLEKE   
Sbjct: 138 SKVLDDFHHNNAFFARVGGVSNAEMNRLELELLAVLDFEVMLSHRVYELYREHLEKEARR 193

Query: 198 NGNG 199
           +G G
Sbjct: 198 DGGG 193

[Close]
BLAST of Cmc11g0293371 vs. ExPASy Swiss-Prot
Match: Q7XC35 (Cyclin-P4-1 OS=Oryza sativa subsp. japonica OX=39947 GN=CYCP4-1 PE=2 SV=1)

HSP 1 Score: 151.8 bits (382), Expect = 9.9e-36
Identity = 77/174 (44.25%), Postives = 109/174 (62.64%), Query Frame = 0 

Query: 19  AEPTPRVLIILAFVLDRLVARNDRLLNELTQQLEELGCCSSSSNHLGNSFNAFHGVRAPT 78
           AE  PRV+ IL+ +L R+  RND                +++      + +AF G+  P 
Sbjct: 7   AEAVPRVVAILSSLLQRVAERND------------AAAAAAAVGEEAAAVSAFQGLTKPA 66

Query: 79  ISILKYLERIYKYTNCSPSCLVVGFVYIDRLIHRHPDSLVISLNVHRLLVTSVMVASKML 138
           ISI  YLERI+++ NCSPSC VV ++Y+DR + R P   V S NVHRLL+TSV+ A K +
Sbjct: 67  ISIGGYLERIFRFANCSPSCYVVAYIYLDRFLRRRPALAVDSFNVHRLLITSVLTAVKFV 126

Query: 139 DDVHYNNAFYARVGGVSKGELNKLELEMLFLLDFGVTVSSRAFETYCCHLEKEM 193
           DD+ YNNA++ARVGG+S  E+N LE++ LF + F + V+  AF +YC  L+ EM
Sbjct: 127 DDICYNNAYFARVGGISLMEMNYLEVDFLFGIAFDLNVTPAAFASYCAVLQSEM 168

[Close]
BLAST of Cmc11g0293371 vs. ExPASy TrEMBL
Match: A0A5D3BEZ8 (Cyclin-U1-1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold232G00280 PE=3 SV=1)

HSP 1 Score: 465.7 bits (1197), Expect = 1.1e-127
Identity = 233/233 (100.00%), Postives = 233/233 (100.00%), Query Frame = 0 

Query: 1   MLTGNDFAAPGRAEGDNAAEPTPRVLIILAFVLDRLVARNDRLLNELTQQLEELGCCSSS 60
           MLTGNDFAAPGRAEGDNAAEPTPRVLIILAFVLDRLVARNDRLLNELTQQLEELGCCSSS
Sbjct: 1   MLTGNDFAAPGRAEGDNAAEPTPRVLIILAFVLDRLVARNDRLLNELTQQLEELGCCSSS 60

Query: 61  SNHLGNSFNAFHGVRAPTISILKYLERIYKYTNCSPSCLVVGFVYIDRLIHRHPDSLVIS 120
           SNHLGNSFNAFHGVRAPTISILKYLERIYKYTNCSPSCLVVGFVYIDRLIHRHPDSLVIS
Sbjct: 61  SNHLGNSFNAFHGVRAPTISILKYLERIYKYTNCSPSCLVVGFVYIDRLIHRHPDSLVIS 120

Query: 121 LNVHRLLVTSVMVASKMLDDVHYNNAFYARVGGVSKGELNKLELEMLFLLDFGVTVSSRA 180
           LNVHRLLVTSVMVASKMLDDVHYNNAFYARVGGVSKGELNKLELEMLFLLDFGVTVSSRA
Sbjct: 121 LNVHRLLVTSVMVASKMLDDVHYNNAFYARVGGVSKGELNKLELEMLFLLDFGVTVSSRA 180

Query: 181 FETYCCHLEKEMLLNGNGEMQRVERPLVMPTNTLDDVLEISVDDTLLVSSSPP 234
           FETYCCHLEKEMLLNGNGEMQRVERPLVMPTNTLDDVLEISVDDTLLVSSSPP
Sbjct: 181 FETYCCHLEKEMLLNGNGEMQRVERPLVMPTNTLDDVLEISVDDTLLVSSSPP 233

[Close]
BLAST of Cmc11g0293371 vs. ExPASy TrEMBL
Match: A0A1S3C1Y7 (cyclin-U1-1 OS=Cucumis melo OX=3656 GN=LOC103495936 PE=3 SV=1)

HSP 1 Score: 465.7 bits (1197), Expect = 1.1e-127
Identity = 233/233 (100.00%), Postives = 233/233 (100.00%), Query Frame = 0 

Query: 1   MLTGNDFAAPGRAEGDNAAEPTPRVLIILAFVLDRLVARNDRLLNELTQQLEELGCCSSS 60
           MLTGNDFAAPGRAEGDNAAEPTPRVLIILAFVLDRLVARNDRLLNELTQQLEELGCCSSS
Sbjct: 1   MLTGNDFAAPGRAEGDNAAEPTPRVLIILAFVLDRLVARNDRLLNELTQQLEELGCCSSS 60

Query: 61  SNHLGNSFNAFHGVRAPTISILKYLERIYKYTNCSPSCLVVGFVYIDRLIHRHPDSLVIS 120
           SNHLGNSFNAFHGVRAPTISILKYLERIYKYTNCSPSCLVVGFVYIDRLIHRHPDSLVIS
Sbjct: 61  SNHLGNSFNAFHGVRAPTISILKYLERIYKYTNCSPSCLVVGFVYIDRLIHRHPDSLVIS 120

Query: 121 LNVHRLLVTSVMVASKMLDDVHYNNAFYARVGGVSKGELNKLELEMLFLLDFGVTVSSRA 180
           LNVHRLLVTSVMVASKMLDDVHYNNAFYARVGGVSKGELNKLELEMLFLLDFGVTVSSRA
Sbjct: 121 LNVHRLLVTSVMVASKMLDDVHYNNAFYARVGGVSKGELNKLELEMLFLLDFGVTVSSRA 180

Query: 181 FETYCCHLEKEMLLNGNGEMQRVERPLVMPTNTLDDVLEISVDDTLLVSSSPP 234
           FETYCCHLEKEMLLNGNGEMQRVERPLVMPTNTLDDVLEISVDDTLLVSSSPP
Sbjct: 181 FETYCCHLEKEMLLNGNGEMQRVERPLVMPTNTLDDVLEISVDDTLLVSSSPP 233

[Close]
BLAST of Cmc11g0293371 vs. ExPASy TrEMBL
Match: A0A0A0LQH0 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G422050 PE=3 SV=1)

HSP 1 Score: 452.2 bits (1162), Expect = 1.3e-123
Identity = 226/233 (97.00%), Postives = 229/233 (98.28%), Query Frame = 0 

Query: 1   MLTGNDFAAPGRAEGDNAAEPTPRVLIILAFVLDRLVARNDRLLNELTQQLEELGCCSSS 60
           MLTG+DFAAPGR EGDNAAEPTPRVLIILAFVLDRLVARNDRLLNEL+QQLEELGCCSSS
Sbjct: 1   MLTGSDFAAPGRVEGDNAAEPTPRVLIILAFVLDRLVARNDRLLNELSQQLEELGCCSSS 60

Query: 61  SNHLGNSFNAFHGVRAPTISILKYLERIYKYTNCSPSCLVVGFVYIDRLIHRHPDSLVIS 120
           SNHLGNSFNAFHGVRAPTISILKYLERIYKYTNCSPSCLVVGFVYIDRLIHRHPDSLVIS
Sbjct: 61  SNHLGNSFNAFHGVRAPTISILKYLERIYKYTNCSPSCLVVGFVYIDRLIHRHPDSLVIS 120

Query: 121 LNVHRLLVTSVMVASKMLDDVHYNNAFYARVGGVSKGELNKLELEMLFLLDFGVTVSSRA 180
           LNVHRLLVTSVMVASKMLDDVHYNNAFYARVGGVSKGELNKLELEMLFLLDFGVTVSSR 
Sbjct: 121 LNVHRLLVTSVMVASKMLDDVHYNNAFYARVGGVSKGELNKLELEMLFLLDFGVTVSSRV 180

Query: 181 FETYCCHLEKEMLLNGNGEMQRVERPLVMPTNTLDDVLEISVDDTLLVSSSPP 234
           FETYC HLEKEMLLNGNGEMQRVERPL+MPTNTLDDV EISVDDTLLVSSSPP
Sbjct: 181 FETYCWHLEKEMLLNGNGEMQRVERPLIMPTNTLDDVSEISVDDTLLVSSSPP 233

[Close]
BLAST of Cmc11g0293371 vs. ExPASy TrEMBL
Match: A0A6J1G7K4 (Cyclin OS=Cucurbita moschata OX=3662 GN=LOC111451542 PE=3 SV=1)

HSP 1 Score: 384.4 bits (986), Expect = 3.3e-103
Identity = 197/233 (84.55%), Postives = 212/233 (90.99%), Query Frame = 0 

Query: 1   MLTGNDFAAPGRAEGDNAAEPTPRVLIILAFVLDRLVARNDRLLNELTQQLEELGCCSSS 60
           MLTGNDF A G AEGDN AEPTPRVLIILA VLDRLVARNDR+++ +T Q+EE   C +S
Sbjct: 1   MLTGNDFPANGLAEGDNVAEPTPRVLIILACVLDRLVARNDRIVDGITHQIEESSSCGTS 60

Query: 61  SNHLGNSFNAFHGVRAPTISILKYLERIYKYTNCSPSCLVVGFVYIDRLIHRHPDSLVIS 120
             H+GNSFNAFHGVRAP ISILKYLERIYKYTNCSPSCLVVGFVYIDRLIHRHP+SLVIS
Sbjct: 61  --HIGNSFNAFHGVRAPAISILKYLERIYKYTNCSPSCLVVGFVYIDRLIHRHPNSLVIS 120

Query: 121 LNVHRLLVTSVMVASKMLDDVHYNNAFYARVGGVSKGELNKLELEMLFLLDFGVTVSSRA 180
           LN+HRLLVTSVMVASK+LDDVHYNNAFYARVGGV+K ELNKLELE+LFLLDFGVTVSSRA
Sbjct: 121 LNIHRLLVTSVMVASKILDDVHYNNAFYARVGGVNKVELNKLELELLFLLDFGVTVSSRA 180

Query: 181 FETYCCHLEKEMLLNGNGEMQRVERPLVMPTNTLDDVLEISVDDTLLVSSSPP 234
           FETYC HLEKEMLLNGNGE+QR+ERPL M TN+LDDV EISVDDT L SSSPP
Sbjct: 181 FETYCLHLEKEMLLNGNGELQRIERPL-MATNSLDDVSEISVDDT-LASSSPP 229

[Close]
BLAST of Cmc11g0293371 vs. ExPASy TrEMBL
Match: A0A6J1I5U7 (Cyclin OS=Cucurbita maxima OX=3661 GN=LOC111469864 PE=3 SV=1)

HSP 1 Score: 383.6 bits (984), Expect = 5.7e-103
Identity = 197/233 (84.55%), Postives = 211/233 (90.56%), Query Frame = 0 

Query: 1   MLTGNDFAAPGRAEGDNAAEPTPRVLIILAFVLDRLVARNDRLLNELTQQLEELGCCSSS 60
           MLTGNDF A G AEGDN AEPTPRVLIILA VLDRLVARNDR+ + +T Q+EE   C +S
Sbjct: 1   MLTGNDFPANGLAEGDNVAEPTPRVLIILACVLDRLVARNDRIADGITHQIEESSSCGTS 60

Query: 61  SNHLGNSFNAFHGVRAPTISILKYLERIYKYTNCSPSCLVVGFVYIDRLIHRHPDSLVIS 120
             H+GNSFNAFHGVRAP ISILKYLERIYKYTNCSPSCLVVGFVYIDRLIHRHP+SLVIS
Sbjct: 61  --HIGNSFNAFHGVRAPAISILKYLERIYKYTNCSPSCLVVGFVYIDRLIHRHPNSLVIS 120

Query: 121 LNVHRLLVTSVMVASKMLDDVHYNNAFYARVGGVSKGELNKLELEMLFLLDFGVTVSSRA 180
           LNVHRLLVTS+MVASK+LDDVHYNNAFYARVGGV+K ELNKLELE+LFLLDFGVTVSSRA
Sbjct: 121 LNVHRLLVTSIMVASKILDDVHYNNAFYARVGGVNKVELNKLELELLFLLDFGVTVSSRA 180

Query: 181 FETYCCHLEKEMLLNGNGEMQRVERPLVMPTNTLDDVLEISVDDTLLVSSSPP 234
           FETYC HLEKEMLLNGNGE+QR+ERPL M TN+LDDV EISVDDT L SSSPP
Sbjct: 181 FETYCLHLEKEMLLNGNGELQRIERPL-MATNSLDDVSEISVDDT-LASSSPP 229

[Close]
BLAST of Cmc11g0293371 vs. TAIR 10
Match: AT3G21870.1 (cyclin p2;1 )

HSP 1 Score: 232.6 bits (592), Expect = 3.1e-61
Identity = 125/222 (56.31%), Postives = 152/222 (68.47%), Query Frame = 0 

Query: 1   MLT--GNDFAAPGRAEGDNAAEPTPRVLIILAFVLDRLVARNDRLLNELTQQLEELGCCS 60
           MLT  G+D   P           TPRVL I++ V+++LVARN+ L              +
Sbjct: 1   MLTAAGDDELDPVVGPESATEAATPRVLTIISHVMEKLVARNEWL--------------A 60

Query: 61  SSSNHLGNSFNAFHGVRAPTISILKYLERIYKYTNCSPSCLVVGFVYIDRLIHRHPDSLV 120
             +   G S  AFHGVRAP+ISI KYLERIYKYT CSP+C VVG+VYIDRL H+HP SLV
Sbjct: 61  KQTKGFGKSLEAFHGVRAPSISIAKYLERIYKYTKCSPACFVVGYVYIDRLAHKHPGSLV 120

Query: 121 ISLNVHRLLVTSVMVASKMLDDVHYNNAFYARVGGVSKGELNKLELEMLFLLDFGVTVSS 180
           +SLNVHRLLVT VM+A+K+LDDVHYNN FYARVGGVS  +LNK+ELE+LFLLDF VTVS 
Sbjct: 121 VSLNVHRLLVTCVMIAAKILDDVHYNNEFYARVGGVSNADLNKMELELLFLLDFRVTVSF 180

Query: 181 RAFETYCCHLEKEMLLNGNGEMQRVERPL---VMPTNTLDDV 218
           R FE+YC HLEKEM LN      +  +P+   + P +TL  +
Sbjct: 181 RVFESYCFHLEKEMQLNDAVSSLKDIQPMQESLSPASTLSSL 208

[Close]
BLAST of Cmc11g0293371 vs. TAIR 10
Match: AT2G44740.1 (cyclin p4;1 )

HSP 1 Score: 158.7 bits (400), Expect = 5.7e-39
Identity = 80/171 (46.78%), Postives = 107/171 (62.57%), Query Frame = 0 

Query: 24  RVLIILAFVLDRLVARNDRLLNELTQQLEELGCCSSSSNHLGNSFNAFHGVRAPTISILK 83
           +++  L+ +L+R+   ND      TQ                   + FHG+  PTI+I  
Sbjct: 12  KLIAFLSSLLERVAESNDLTRRVATQ---------------SQRVSVFHGLSRPTITIQS 71

Query: 84  YLERIYKYTNCSPSCLVVGFVYIDRLIHRHPDSLVISLNVHRLLVTSVMVASKMLDDVHY 143
           YLERI+KY NCSPSC VV +VY+DR  HR P   + S NVHRLL+TSVMVA+K LDD++Y
Sbjct: 72  YLERIFKYANCSPSCFVVAYVYLDRFTHRQPSLPINSFNVHRLLITSVMVAAKFLDDLYY 131

Query: 144 NNAFYARVGGVSKGELNKLELEMLFLLDFGVTVSSRAFETYCCHLEKEMLL 195
           NNA+YA+VGG+S  E+N LEL+ LF L F + V+   F  Y  +L+KEM L
Sbjct: 132 NNAYYAKVGGISTKEMNFLELDFLFGLGFELNVTPNTFNAYFSYLQKEMTL 167

[Close]
BLAST of Cmc11g0293371 vs. TAIR 10
Match: AT5G61650.1 (CYCLIN P4;2 )

HSP 1 Score: 146.7 bits (369), Expect = 2.3e-35
Identity = 71/176 (40.34%), Postives = 108/176 (61.36%), Query Frame = 0 

Query: 19  AEPTPRVLIILAFVLDRLVARNDRLLNELTQQLEELGCCSSSSNHLGNSFNAFHGVRAPT 78
           AE  P VL  ++++L R+   ND L  +                      ++F GV  P+
Sbjct: 19  AEIMPSVLTAMSYLLQRVSETNDNLSQK-------------------QKPSSFTGVTKPS 78

Query: 79  ISILKYLERIYKYTNCSPSCLVVGFVYIDRLIHRHPDSLVISLNVHRLLVTSVMVASKML 138
           ISI  YLERI++Y NCS SC +V ++Y+DR + + P   + S NVHRL++TSV+V++K +
Sbjct: 79  ISIRSYLERIFEYANCSYSCYIVAYIYLDRFVKKQPFLPINSFNVHRLIITSVLVSAKFM 138

Query: 139 DDVHYNNAFYARVGGVSKGELNKLELEMLFLLDFGVTVSSRAFETYCCHLEKEMLL 195
           DD+ YNN +YA+VGG+S+ E+N LEL+ LF + F + V+   F  YCC L++EM +
Sbjct: 139 DDLSYNNEYYAKVGGISREEMNMLELDFLFGIGFELNVTVSTFNNYCCFLQREMAM 175

[Close]
BLAST of Cmc11g0293371 vs. TAIR 10
Match: AT3G60550.1 (cyclin p3;2 )

HSP 1 Score: 144.8 bits (364), Expect = 8.6e-35
Identity = 71/184 (38.59%), Postives = 112/184 (60.87%), Query Frame = 0 

Query: 22  TPRVLIILAFVLDRLVARNDRLLNELTQQLEELGCCSSSSNHLGNSFNAFHGVRAPTISI 81
           TP V+ +L+ ++DR + RN+R+              +  S+  G     F     P ++I
Sbjct: 29  TPLVISVLSSLIDRTLTRNERISRR-----------ALPSSGAGGKTQIFDCREIPDMTI 88

Query: 82  LKYLERIYKYTNCSPSCLVVGFVYIDRLIHRHPDSLVISLNVHRLLVTSVMVASKMLDDV 141
             YL RI++YT   PS  VV +VYIDR    +P   +   NVHRLL+T++M+ASK ++D+
Sbjct: 89  QSYLGRIFRYTKAGPSVYVVAYVYIDRFCQTNPGFRISLTNVHRLLITTIMIASKYVEDL 148

Query: 142 HYNNAFYARVGGVSKGELNKLELEMLFLLDFGVTVSSRAFETYCCHLEKEMLLNGNGEMQ 201
           +Y N+++A+VGG+   +LNKLELE LFL+ F + V+   FE+YCCHLE+E+   G  +++
Sbjct: 149 NYRNSYFAKVGGLETEDLNKLELEFLFLMGFKLHVNVSVFESYCCHLEREVSFGGGYQIE 201

Query: 202 RVER 206
           +  R
Sbjct: 209 KALR 201

[Close]
BLAST of Cmc11g0293371 vs. TAIR 10
Match: AT3G63120.1 (cyclin p1;1 )

HSP 1 Score: 143.7 bits (361), Expect = 1.9e-34
Identity = 66/126 (52.38%), Postives = 93/126 (73.81%), Query Frame = 0 

Query: 66  NSFNAFHGVRAPTISILKYLERIYKYTNCSPSCLVVGFVYIDRLIHRHPDSLVISLNVHR 125
           +S   F G   P ISI  YL+RI+KY+ CSPSC V+  +YID  +H+   +L+  LNVHR
Sbjct: 60  DSVTVFDGRSPPEISIAHYLDRIFKYSCCSPSCFVIAHIYIDHFLHK-TRALLKPLNVHR 119

Query: 126 LLVTSVMVASKMLDDVHYNNAFYARVGGVSKGELNKLELEMLFLLDFGVTVSSRAFETYC 185
           L++T+VM+A+K+ DD ++NNA+YARVGGV+  ELN+LE+E+LF LDF + V  + F T+C
Sbjct: 120 LIITTVMLAAKVFDDRYFNNAYYARVGGVTTRELNRLEMELLFTLDFKLQVDPQTFHTHC 179

Query: 186 CHLEKE 192
           C LEK+
Sbjct: 180 CQLEKQ 184

The following BLAST results are available for this feature:
BLAST of Cmc11g0293371 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_008455834.12.4e-127100.00PREDICTED: cyclin-U1-1 [Cucumis melo] >KAA0042430.1 cyclin-U1-1 [Cucumis melo va... [more]
KAE8652302.12.7e-12397.00hypothetical protein Csa_022481 [Cucumis sativus][more]
XP_004151930.22.7e-12397.00cyclin-U1-1 [Cucumis sativus][more]
XP_038901956.11.1e-11391.91cyclin-U1-1 [Benincasa hispida][more]
KAG6604845.11.1e-10384.98Cyclin-U1-1, partial [Cucurbita argyrosperma subsp. sororia][more]
BLAST of Cmc11g0293371 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q9LJ454.4e-6056.31Cyclin-U1-1 OS=Arabidopsis thaliana OX=3702 GN=CYCU1-1 PE=1 SV=1[more]
Q75HV08.1e-3847.67Cyclin-P3-1 OS=Oryza sativa subsp. japonica OX=39947 GN=CYCP3-1 PE=3 SV=1[more]
O805138.1e-3846.78Cyclin-U4-1 OS=Arabidopsis thaliana OX=3702 GN=CYCU4-1 PE=1 SV=1[more]
Q0J9W01.8e-3746.20Cyclin-P1-1 OS=Oryza sativa subsp. japonica OX=39947 GN=CYCP1-1 PE=3 SV=2[more]
Q7XC359.9e-3644.25Cyclin-P4-1 OS=Oryza sativa subsp. japonica OX=39947 GN=CYCP4-1 PE=2 SV=1[more]
BLAST of Cmc11g0293371 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A5D3BEZ81.1e-127100.00Cyclin-U1-1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold232G00280 PE... [more]
A0A1S3C1Y71.1e-127100.00cyclin-U1-1 OS=Cucumis melo OX=3656 GN=LOC103495936 PE=3 SV=1[more]
A0A0A0LQH01.3e-12397.00Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G422050 PE=3 SV=1[more]
A0A6J1G7K43.3e-10384.55Cyclin OS=Cucurbita moschata OX=3662 GN=LOC111451542 PE=3 SV=1[more]
A0A6J1I5U75.7e-10384.55Cyclin OS=Cucurbita maxima OX=3661 GN=LOC111469864 PE=3 SV=1[more]
BLAST of Cmc11g0293371 vs. TAIR 10
Match NameE-valueIdentityDescription
AT3G21870.13.1e-6156.31cyclin p2;1 [more]
AT2G44740.15.7e-3946.78cyclin p4;1 [more]
AT5G61650.12.3e-3540.34CYCLIN P4;2 [more]
AT3G60550.18.6e-3538.59cyclin p3;2 [more]
AT3G63120.11.9e-3452.38cyclin p1;1 [more]
InterPro
Analysis Name: InterPro Annotations of Melon (Charmono) v1.1
Date Performed: 2022-10-13
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 33..53
NoneNo IPR availableGENE3D1.10.472.10coord: 16..215
e-value: 7.2E-53
score: 181.3
NoneNo IPR availablePANTHERPTHR15615:SF108PROTEIN CNPPD1coord: 16..199
NoneNo IPR availablePANTHERPTHR15615UNCHARACTERIZEDcoord: 16..199
IPR013922Cyclin PHO80-likePFAMPF08613Cyclincoord: 27..173
e-value: 1.6E-35
score: 122.9
IPR013763Cyclin-likeCDDcd00043CYCLINcoord: 79..167
e-value: 5.90673E-4
score: 36.0625
IPR036915Cyclin-like superfamilySUPERFAMILY47954Cyclin-likecoord: 81..174

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cmc11g0293371.1Cmc11g0293371.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0000079 regulation of cyclin-dependent protein serine/threonine kinase activity
molecular_function GO:0019901 protein kinase binding