Homology
BLAST of Cmc09g0259781 vs. NCBI nr
Match:
XP_008437519.1 (PREDICTED: cucumisin-like isoform X1 [Cucumis melo])
HSP 1 Score: 1493.0 bits (3864), Expect = 0.0e+00
Identity = 744/746 (99.73%), Postives = 745/746 (99.87%), Query Frame = 0
Query: 1 MSSLSRLLFLCFCFSLLFFSSLSEEDDQYRKTYIVYMGSHPKDQVSSTPLSSHHHMRLLQ 60
MSSLSRLLFLCFCFSLLFFSSLSEEDDQYRKTYIVYMGSHPKDQVSSTPLSSHHHMRLLQ
Sbjct: 1 MSSLSRLLFLCFCFSLLFFSSLSEEDDQYRKTYIVYMGSHPKDQVSSTPLSSHHHMRLLQ 60
Query: 61 EAIGSTFAPHCLLHSYKRSFNGFVAKLTETEAKKVSEMEGVISVFPNEELQLHTTRSWDF 120
EAIGSTFAPHCLLHSYKRSFNGFVAKLTETEAKKVSEMEGVISVFPNEELQLHTTRSWDF
Sbjct: 61 EAIGSTFAPHCLLHSYKRSFNGFVAKLTETEAKKVSEMEGVISVFPNEELQLHTTRSWDF 120
Query: 121 MGFSEQVERVPSVESDIIVGVFDTGIWPESPSFLDHGYGPPPPKWKGSCEVSLNFSCNNK 180
MGFSEQVERVPSVESDIIVGVFDTGIWPESPSFLDHGYGPPPPKWKGSCEVSLNFSCNNK
Sbjct: 121 MGFSEQVERVPSVESDIIVGVFDTGIWPESPSFLDHGYGPPPPKWKGSCEVSLNFSCNNK 180
Query: 181 IIGAQSYRSDGRYPIDDIKGPRDSNGHGTHAASTVAGGLVRQASMLGLGTGTARGGVPSA 240
IIGAQSYRSDGRYPIDDIKGPRDSNGHGTHAASTVAGGLVRQASMLGLGTGTARGGVPSA
Sbjct: 181 IIGAQSYRSDGRYPIDDIKGPRDSNGHGTHAASTVAGGLVRQASMLGLGTGTARGGVPSA 240
Query: 241 RIAAYKVCWSDTCSGADVLAAFDDAIADGVDIISLSVGPKRPIPNYFNDPIAIGTFHAMR 300
RIAAYKVCWSDTCSGADVLAAFDDAIADGVDIISLSVGPKRPIPNYFNDPIAIGTFHAMR
Sbjct: 241 RIAAYKVCWSDTCSGADVLAAFDDAIADGVDIISLSVGPKRPIPNYFNDPIAIGTFHAMR 300
Query: 301 NGILTSSSAGNEGPRPFTLTNFSPWALSVAASTTDRRFLTAVQLGDGRKFNGVTINTFDL 360
NGILTSSSAGNEGPRPFTLTNFSPWALSVAASTTDRRFLTAVQLGDGRKFNGVTINTFDL
Sbjct: 301 NGILTSSSAGNEGPRPFTLTNFSPWALSVAASTTDRRFLTAVQLGDGRKFNGVTINTFDL 360
Query: 361 NGTQYPLVHAGNIPNVTGGFNGSISRFCLGNSVDRELVKGKIAVCDSIVLPSDVGSLESA 420
NGTQYPLVHAGNIPNVTGGFNGSISRFCLGNSVDRELVKGKIAVCDSIVLPSDVGSLESA
Sbjct: 361 NGTQYPLVHAGNIPNVTGGFNGSISRFCLGNSVDRELVKGKIAVCDSIVLPSDVGSLESA 420
Query: 421 LGIIMQDGSPKDLTAAFPLPASHLGTQQRPLISSYLNLTRIPTATILKSTELKLEAAPLV 480
LGIIMQDGSPKDLTAAFPLPASHLGTQQRPLISSYLNLTRIPTATILKSTELKLEAAPLV
Sbjct: 421 LGIIMQDGSPKDLTAAFPLPASHLGTQQRPLISSYLNLTRIPTATILKSTELKLEAAPLV 480
Query: 481 ASFSSRGPNPTTPDILKPDVIGPGVEILAAWSPIRSPSDAKGDNRKLLFNIISGTSMACP 540
ASFSSRGPNPTTPDILKPDVIGPGVEILAAWSPIRSPSDAKGDNRKLLFNIISGTSMACP
Sbjct: 481 ASFSSRGPNPTTPDILKPDVIGPGVEILAAWSPIRSPSDAKGDNRKLLFNIISGTSMACP 540
Query: 541 HATAVAAYVKSFHPSWSPAALKSALITTAFPMRGDLYPEAEFAYGSGHINPLGAVNPGLI 600
HATAVAAYVKSFHPSWSPAALKSALITTAFPMRGDLYPEAEFAYGSGHINPLGAVNPGLI
Sbjct: 541 HATAVAAYVKSFHPSWSPAALKSALITTAFPMRGDLYPEAEFAYGSGHINPLGAVNPGLI 600
Query: 601 YNASEIDYIRFLCGEGYNTTLLRIITKDNSTCSPTNSGLVYDLNYPSFALFTYISTPFSQ 660
YNASEIDYIRFLCGEGYNTTLLRIITKDNSTCSPTNSGLVYDLNY SFALFTYISTPFSQ
Sbjct: 601 YNASEIDYIRFLCGEGYNTTLLRIITKDNSTCSPTNSGLVYDLNYHSFALFTYISTPFSQ 660
Query: 661 VYKRKVTNVGSTNSTYKATIFAPSELNITVNPSTLSFKALEEELKFEVTIEGKINGSIAS 720
VYKR+VTNVGSTNSTYKATIFAPSELNITVNPSTLSFKALEEELKFEVTIEGKINGSIAS
Sbjct: 661 VYKRRVTNVGSTNSTYKATIFAPSELNITVNPSTLSFKALEEELKFEVTIEGKINGSIAS 720
Query: 721 ASLVWDDGVHKVRSPIIVFDSDTFPR 747
ASLVWDDGVHKVRSPIIVFDSDTFPR
Sbjct: 721 ASLVWDDGVHKVRSPIIVFDSDTFPR 746
BLAST of Cmc09g0259781 vs. NCBI nr
Match:
XP_008437521.1 (PREDICTED: cucumisin-like isoform X2 [Cucumis melo])
HSP 1 Score: 1421.0 bits (3677), Expect = 0.0e+00
Identity = 706/708 (99.72%), Postives = 707/708 (99.86%), Query Frame = 0
Query: 39 SHPKDQVSSTPLSSHHHMRLLQEAIGSTFAPHCLLHSYKRSFNGFVAKLTETEAKKVSEM 98
SHPKDQVSSTPLSSHHHMRLLQEAIGSTFAPHCLLHSYKRSFNGFVAKLTETEAKKVSEM
Sbjct: 6 SHPKDQVSSTPLSSHHHMRLLQEAIGSTFAPHCLLHSYKRSFNGFVAKLTETEAKKVSEM 65
Query: 99 EGVISVFPNEELQLHTTRSWDFMGFSEQVERVPSVESDIIVGVFDTGIWPESPSFLDHGY 158
EGVISVFPNEELQLHTTRSWDFMGFSEQVERVPSVESDIIVGVFDTGIWPESPSFLDHGY
Sbjct: 66 EGVISVFPNEELQLHTTRSWDFMGFSEQVERVPSVESDIIVGVFDTGIWPESPSFLDHGY 125
Query: 159 GPPPPKWKGSCEVSLNFSCNNKIIGAQSYRSDGRYPIDDIKGPRDSNGHGTHAASTVAGG 218
GPPPPKWKGSCEVSLNFSCNNKIIGAQSYRSDGRYPIDDIKGPRDSNGHGTHAASTVAGG
Sbjct: 126 GPPPPKWKGSCEVSLNFSCNNKIIGAQSYRSDGRYPIDDIKGPRDSNGHGTHAASTVAGG 185
Query: 219 LVRQASMLGLGTGTARGGVPSARIAAYKVCWSDTCSGADVLAAFDDAIADGVDIISLSVG 278
LVRQASMLGLGTGTARGGVPSARIAAYKVCWSDTCSGADVLAAFDDAIADGVDIISLSVG
Sbjct: 186 LVRQASMLGLGTGTARGGVPSARIAAYKVCWSDTCSGADVLAAFDDAIADGVDIISLSVG 245
Query: 279 PKRPIPNYFNDPIAIGTFHAMRNGILTSSSAGNEGPRPFTLTNFSPWALSVAASTTDRRF 338
PKRPIPNYFNDPIAIGTFHAMRNGILTSSSAGNEGPRPFTLTNFSPWALSVAASTTDRRF
Sbjct: 246 PKRPIPNYFNDPIAIGTFHAMRNGILTSSSAGNEGPRPFTLTNFSPWALSVAASTTDRRF 305
Query: 339 LTAVQLGDGRKFNGVTINTFDLNGTQYPLVHAGNIPNVTGGFNGSISRFCLGNSVDRELV 398
LTAVQLGDGRKFNGVTINTFDLNGTQYPLVHAGNIPNVTGGFNGSISRFCLGNSVDRELV
Sbjct: 306 LTAVQLGDGRKFNGVTINTFDLNGTQYPLVHAGNIPNVTGGFNGSISRFCLGNSVDRELV 365
Query: 399 KGKIAVCDSIVLPSDVGSLESALGIIMQDGSPKDLTAAFPLPASHLGTQQRPLISSYLNL 458
KGKIAVCDSIVLPSDVGSLESALGIIMQDGSPKDLTAAFPLPASHLGTQQRPLISSYLNL
Sbjct: 366 KGKIAVCDSIVLPSDVGSLESALGIIMQDGSPKDLTAAFPLPASHLGTQQRPLISSYLNL 425
Query: 459 TRIPTATILKSTELKLEAAPLVASFSSRGPNPTTPDILKPDVIGPGVEILAAWSPIRSPS 518
TRIPTATILKSTELKLEAAPLVASFSSRGPNPTTPDILKPDVIGPGVEILAAWSPIRSPS
Sbjct: 426 TRIPTATILKSTELKLEAAPLVASFSSRGPNPTTPDILKPDVIGPGVEILAAWSPIRSPS 485
Query: 519 DAKGDNRKLLFNIISGTSMACPHATAVAAYVKSFHPSWSPAALKSALITTAFPMRGDLYP 578
DAKGDNRKLLFNIISGTSMACPHATAVAAYVKSFHPSWSPAALKSALITTAFPMRGDLYP
Sbjct: 486 DAKGDNRKLLFNIISGTSMACPHATAVAAYVKSFHPSWSPAALKSALITTAFPMRGDLYP 545
Query: 579 EAEFAYGSGHINPLGAVNPGLIYNASEIDYIRFLCGEGYNTTLLRIITKDNSTCSPTNSG 638
EAEFAYGSGHINPLGAVNPGLIYNASEIDYIRFLCGEGYNTTLLRIITKDNSTCSPTNSG
Sbjct: 546 EAEFAYGSGHINPLGAVNPGLIYNASEIDYIRFLCGEGYNTTLLRIITKDNSTCSPTNSG 605
Query: 639 LVYDLNYPSFALFTYISTPFSQVYKRKVTNVGSTNSTYKATIFAPSELNITVNPSTLSFK 698
LVYDLNY SFALFTYISTPFSQVYKR+VTNVGSTNSTYKATIFAPSELNITVNPSTLSFK
Sbjct: 606 LVYDLNYHSFALFTYISTPFSQVYKRRVTNVGSTNSTYKATIFAPSELNITVNPSTLSFK 665
Query: 699 ALEEELKFEVTIEGKINGSIASASLVWDDGVHKVRSPIIVFDSDTFPR 747
ALEEELKFEVTIEGKINGSIASASLVWDDGVHKVRSPIIVFDSDTFPR
Sbjct: 666 ALEEELKFEVTIEGKINGSIASASLVWDDGVHKVRSPIIVFDSDTFPR 713
BLAST of Cmc09g0259781 vs. NCBI nr
Match:
XP_008437522.1 (PREDICTED: cucumisin-like isoform X3 [Cucumis melo])
HSP 1 Score: 1370.1 bits (3545), Expect = 0.0e+00
Identity = 681/684 (99.56%), Postives = 682/684 (99.71%), Query Frame = 0
Query: 63 IGSTFAPHCLLHSYKRSFNGFVAKLTETEAKKVSEMEGVISVFPNEELQLHTTRSWDFMG 122
I STFAPHCLLHSYKRSFNGFVAKLTETEAKKVSEMEGVISVFPNEELQLHTTRSWDFMG
Sbjct: 4 IESTFAPHCLLHSYKRSFNGFVAKLTETEAKKVSEMEGVISVFPNEELQLHTTRSWDFMG 63
Query: 123 FSEQVERVPSVESDIIVGVFDTGIWPESPSFLDHGYGPPPPKWKGSCEVSLNFSCNNKII 182
FSEQVERVPSVESDIIVGVFDTGIWPESPSFLDHGYGPPPPKWKGSCEVSLNFSCNNKII
Sbjct: 64 FSEQVERVPSVESDIIVGVFDTGIWPESPSFLDHGYGPPPPKWKGSCEVSLNFSCNNKII 123
Query: 183 GAQSYRSDGRYPIDDIKGPRDSNGHGTHAASTVAGGLVRQASMLGLGTGTARGGVPSARI 242
GAQSYRSDGRYPIDDIKGPRDSNGHGTHAASTVAGGLVRQASMLGLGTGTARGGVPSARI
Sbjct: 124 GAQSYRSDGRYPIDDIKGPRDSNGHGTHAASTVAGGLVRQASMLGLGTGTARGGVPSARI 183
Query: 243 AAYKVCWSDTCSGADVLAAFDDAIADGVDIISLSVGPKRPIPNYFNDPIAIGTFHAMRNG 302
AAYKVCWSDTCSGADVLAAFDDAIADGVDIISLSVGPKRPIPNYFNDPIAIGTFHAMRNG
Sbjct: 184 AAYKVCWSDTCSGADVLAAFDDAIADGVDIISLSVGPKRPIPNYFNDPIAIGTFHAMRNG 243
Query: 303 ILTSSSAGNEGPRPFTLTNFSPWALSVAASTTDRRFLTAVQLGDGRKFNGVTINTFDLNG 362
ILTSSSAGNEGPRPFTLTNFSPWALSVAASTTDRRFLTAVQLGDGRKFNGVTINTFDLNG
Sbjct: 244 ILTSSSAGNEGPRPFTLTNFSPWALSVAASTTDRRFLTAVQLGDGRKFNGVTINTFDLNG 303
Query: 363 TQYPLVHAGNIPNVTGGFNGSISRFCLGNSVDRELVKGKIAVCDSIVLPSDVGSLESALG 422
TQYPLVHAGNIPNVTGGFNGSISRFCLGNSVDRELVKGKIAVCDSIVLPSDVGSLESALG
Sbjct: 304 TQYPLVHAGNIPNVTGGFNGSISRFCLGNSVDRELVKGKIAVCDSIVLPSDVGSLESALG 363
Query: 423 IIMQDGSPKDLTAAFPLPASHLGTQQRPLISSYLNLTRIPTATILKSTELKLEAAPLVAS 482
IIMQDGSPKDLTAAFPLPASHLGTQQRPLISSYLNLTRIPTATILKSTELKLEAAPLVAS
Sbjct: 364 IIMQDGSPKDLTAAFPLPASHLGTQQRPLISSYLNLTRIPTATILKSTELKLEAAPLVAS 423
Query: 483 FSSRGPNPTTPDILKPDVIGPGVEILAAWSPIRSPSDAKGDNRKLLFNIISGTSMACPHA 542
FSSRGPNPTTPDILKPDVIGPGVEILAAWSPIRSPSDAKGDNRKLLFNIISGTSMACPHA
Sbjct: 424 FSSRGPNPTTPDILKPDVIGPGVEILAAWSPIRSPSDAKGDNRKLLFNIISGTSMACPHA 483
Query: 543 TAVAAYVKSFHPSWSPAALKSALITTAFPMRGDLYPEAEFAYGSGHINPLGAVNPGLIYN 602
TAVAAYVKSFHPSWSPAALKSALITTAFPMRGDLYPEAEFAYGSGHINPLGAVNPGLIYN
Sbjct: 484 TAVAAYVKSFHPSWSPAALKSALITTAFPMRGDLYPEAEFAYGSGHINPLGAVNPGLIYN 543
Query: 603 ASEIDYIRFLCGEGYNTTLLRIITKDNSTCSPTNSGLVYDLNYPSFALFTYISTPFSQVY 662
ASEIDYIRFLCGEGYNTTLLRIITKDNSTCSPTNSGLVYDLNY SFALFTYISTPFSQVY
Sbjct: 544 ASEIDYIRFLCGEGYNTTLLRIITKDNSTCSPTNSGLVYDLNYHSFALFTYISTPFSQVY 603
Query: 663 KRKVTNVGSTNSTYKATIFAPSELNITVNPSTLSFKALEEELKFEVTIEGKINGSIASAS 722
KR+VTNVGSTNSTYKATIFAPSELNITVNPSTLSFKALEEELKFEVTIEGKINGSIASAS
Sbjct: 604 KRRVTNVGSTNSTYKATIFAPSELNITVNPSTLSFKALEEELKFEVTIEGKINGSIASAS 663
Query: 723 LVWDDGVHKVRSPIIVFDSDTFPR 747
LVWDDGVHKVRSPIIVFDSDTFPR
Sbjct: 664 LVWDDGVHKVRSPIIVFDSDTFPR 687
BLAST of Cmc09g0259781 vs. NCBI nr
Match:
XP_004145974.1 (cucumisin [Cucumis sativus] >KGN49930.1 hypothetical protein Csa_000282 [Cucumis sativus])
HSP 1 Score: 1351.3 bits (3496), Expect = 0.0e+00
Identity = 681/746 (91.29%), Postives = 701/746 (93.97%), Query Frame = 0
Query: 1 MSSLSRLLFLCFCFSLLFFSSLSEEDDQYRKTYIVYMGSHPKDQVSSTPLSSHHHMRLLQ 60
MSSLSRLLFLC CFSLLF +S+S EDDQYRKTYIVYMGSH QVSS PLSSHHHMR+LQ
Sbjct: 1 MSSLSRLLFLCLCFSLLFSTSVS-EDDQYRKTYIVYMGSH--HQVSSAPLSSHHHMRILQ 60
Query: 61 EAIGSTFAPHCLLHSYKRSFNGFVAKLTETEAKKVSEMEGVISVFPNEELQLHTTRSWDF 120
EA+GSTFAPHCLLHSYKRSFNGFVAKLTE EAKKVSEMEGVISVFPN ELQLHTTRSWDF
Sbjct: 61 EAVGSTFAPHCLLHSYKRSFNGFVAKLTEIEAKKVSEMEGVISVFPNGELQLHTTRSWDF 120
Query: 121 MGFSEQVERVPSVESDIIVGVFDTGIWPESPSFLDHGYGPPPPKWKGSCEVSLNFSCNNK 180
MG SEQVERVPSVESDIIVGVFDTGIWPESPSFLDHGYGPPPPKWKGSCEVS NFSCNNK
Sbjct: 121 MGMSEQVERVPSVESDIIVGVFDTGIWPESPSFLDHGYGPPPPKWKGSCEVSANFSCNNK 180
Query: 181 IIGAQSYRSDGRYPIDDIKGPRDSNGHGTHAASTVAGGLVRQASMLGLGTGTARGGVPSA 240
IIGA+SYRSDGRYPIDDIKGPRDSNGHGTHAASTVAGGLVRQASMLGLG GTARGGVPSA
Sbjct: 181 IIGARSYRSDGRYPIDDIKGPRDSNGHGTHAASTVAGGLVRQASMLGLGMGTARGGVPSA 240
Query: 241 RIAAYKVCWSDTCSGADVLAAFDDAIADGVDIISLSVGPKRPIPNYFNDPIAIGTFHAMR 300
RIAAYKVCWSDTCS ADVLAAFDDAIADGVDIIS+SVGPKRP PNYF DPIAIGTFHAMR
Sbjct: 241 RIAAYKVCWSDTCSDADVLAAFDDAIADGVDIISMSVGPKRPRPNYFQDPIAIGTFHAMR 300
Query: 301 NGILTSSSAGNEGPRPFTLTNFSPWALSVAASTTDRRFLTAVQLGDGRKFNGVTINTFDL 360
NGILTS+SAGNEGP FT+TNFSPWALSVAAST+DRRFLTAVQLGDGRKFNGVTINTFDL
Sbjct: 301 NGILTSTSAGNEGPLHFTVTNFSPWALSVAASTSDRRFLTAVQLGDGRKFNGVTINTFDL 360
Query: 361 NGTQYPLVHAGNIPNVTGGFNGSISRFCLGNSVDRELVKGKIAVCDSIVLPSDVGSLESA 420
NGTQYPLV+AGNIPNVTGGFNGS SRFCL +SVDRELVKGKIA+CDS V PSDVGSLESA
Sbjct: 361 NGTQYPLVYAGNIPNVTGGFNGSFSRFCLRDSVDRELVKGKIAICDSFVSPSDVGSLESA 420
Query: 421 LGIIMQDGSPKDLTAAFPLPASHLGTQQRPLISSYLNLTRIPTATILKSTELKLEAAPLV 480
+GIIMQD SPKDLT AFPLPASHLG QQRPLISSYLN TRIPTATILKST LKL+ APLV
Sbjct: 421 VGIIMQDRSPKDLTFAFPLPASHLGIQQRPLISSYLNSTRIPTATILKSTGLKLQVAPLV 480
Query: 481 ASFSSRGPNPTTPDILKPDVIGPGVEILAAWSPIRSPSDAKGDNRKLLFNIISGTSMACP 540
ASFSSRGPNPT+P ILKPDVIGPGVEILAAWSP+RSPS+AKGDNRKLLFNIISGTSMACP
Sbjct: 481 ASFSSRGPNPTSPYILKPDVIGPGVEILAAWSPLRSPSNAKGDNRKLLFNIISGTSMACP 540
Query: 541 HATAVAAYVKSFHPSWSPAALKSALITTAFPMRGDLYPEAEFAYGSGHINPLGAVNPGLI 600
HATAVAAYVKSFHPSWSPAALKSALITTAFPMRGDLYPEAEFAYGSGHINPLGAVNPGLI
Sbjct: 541 HATAVAAYVKSFHPSWSPAALKSALITTAFPMRGDLYPEAEFAYGSGHINPLGAVNPGLI 600
Query: 601 YNASEIDYIRFLCGEGYNTTLLRIITKDNSTCSPTNSGLVYDLNYPSFALFTYISTPFSQ 660
YNASE DYIRFLC EGYNTT LRIITKDNSTCS T S VYDLNYPSFALFT+ISTPFSQ
Sbjct: 601 YNASETDYIRFLCDEGYNTTFLRIITKDNSTCSTTQSIRVYDLNYPSFALFTHISTPFSQ 660
Query: 661 VYKRKVTNVGSTNSTYKATIFAPSELNITVNPSTLSFKALEEELKFEVTIEGKINGSIAS 720
KR+VTNVGSTNSTYKATI APS LNITVNPS LSFKALEEEL FEVT EGKI+ SI S
Sbjct: 661 TSKRRVTNVGSTNSTYKATISAPSGLNITVNPSILSFKALEEELNFEVTFEGKIDRSIES 720
Query: 721 ASLVWDDGVHKVRSPIIVFDSDTFPR 747
ASLVWDDGVHKVRSPIIVFDSDTF R
Sbjct: 721 ASLVWDDGVHKVRSPIIVFDSDTFTR 743
BLAST of Cmc09g0259781 vs. NCBI nr
Match:
XP_038874300.1 (cucumisin-like [Benincasa hispida])
HSP 1 Score: 1249.6 bits (3232), Expect = 0.0e+00
Identity = 623/744 (83.74%), Postives = 678/744 (91.13%), Query Frame = 0
Query: 1 MSSLSRLLFLCFCFSLLFFSSLSEEDDQYRKTYIVYMGSHPKDQVSSTPLSSHHHMRLLQ 60
MSSLS LLFL FCFSLLFF S+SE+D RKTYIVYMGSHPK QVS +S HHMR+LQ
Sbjct: 1 MSSLSSLLFLSFCFSLLFFCSISEDDQ--RKTYIVYMGSHPKHQVS----TSSHHMRMLQ 60
Query: 61 EAIGSTFAPHCLLHSYKRSFNGFVAKLTETEAKKVSEMEGVISVFPNEELQLHTTRSWDF 120
EAIGSTFAPH LLHSYKRSFNGFVAKLTETE KKVSEM+ VISVF NE++QLHTTRSWDF
Sbjct: 61 EAIGSTFAPHSLLHSYKRSFNGFVAKLTETEVKKVSEMKDVISVFLNEKIQLHTTRSWDF 120
Query: 121 MGFSEQVERVPSVESDIIVGVFDTGIWPESPSFLDHGYGPPPPKWKGSCEVSLNFSCNNK 180
MG ++QV RVPSVESDIIVGVFDTGIWPESPSFLDHGYGPPPPKWKGSCEVS NFSCNNK
Sbjct: 121 MGLTQQVNRVPSVESDIIVGVFDTGIWPESPSFLDHGYGPPPPKWKGSCEVSSNFSCNNK 180
Query: 181 IIGAQSYRSDGRYPIDDIKGPRDSNGHGTHAASTVAGGLVRQASMLGLGTGTARGGVPSA 240
IIGA+SYR +G+YPI+DIKGPRDSNGHGTH ASTVAGGLVRQASMLGLG+GTARGGVPSA
Sbjct: 181 IIGARSYRRNGQYPINDIKGPRDSNGHGTHVASTVAGGLVRQASMLGLGSGTARGGVPSA 240
Query: 241 RIAAYKVCWSDTCSGADVLAAFDDAIADGVDIISLSVGPKRPIPNYFNDPIAIGTFHAMR 300
RIAAYKVCWSDTC D+LAAFDDAIADGVDIIS+S+GP++PI NYF+DPIAIGTFHAM+
Sbjct: 241 RIAAYKVCWSDTCFSVDILAAFDDAIADGVDIISMSLGPRKPIANYFSDPIAIGTFHAMK 300
Query: 301 NGILTSSSAGNEGPRPFTLTNFSPWALSVAASTTDRRFLTAVQLGDGRKFNGVTINTFDL 360
NGILTS+SAGN+GP FTLTNFSPW+L+VAASTTDR+F+T VQLGDGR+FNGVTINTFDL
Sbjct: 301 NGILTSTSAGNDGPSSFTLTNFSPWSLTVAASTTDRKFVTGVQLGDGRRFNGVTINTFDL 360
Query: 361 NGTQYPLVHAGNIPNVTGGFNGSISRFCLGNSVDRELVKGKIAVCDSIVLPSDVGSLESA 420
NGTQYP V+AGN+PNVTGGFNGSISRFCLGN+VDRELVKGKIA+CD +V + +GS+E A
Sbjct: 361 NGTQYPFVYAGNVPNVTGGFNGSISRFCLGNTVDRELVKGKIALCDILVPQTLLGSIEGA 420
Query: 421 LGIIMQDGSPKDLTAAFPLPASHLGTQQRPLISSYLNLTRIPTATILKSTELKLEAAPLV 480
+GIIMQD SPKDLT +FPLPASHLGTQ+ LISSYLNLT +PTATI KS E K EAAP V
Sbjct: 421 VGIIMQDKSPKDLTFSFPLPASHLGTQEGALISSYLNLTSLPTATISKSIEGKHEAAPFV 480
Query: 481 ASFSSRGPNPTTPDILKPDVIGPGVEILAAWSPIRSPSDAKGDNRKLLFNIISGTSMACP 540
ASFSSRGPNPTTP+ILKPD+ GPGVEILAAWSPI SPS AK DNRKLLFNIISGTSMACP
Sbjct: 481 ASFSSRGPNPTTPNILKPDLSGPGVEILAAWSPISSPSTAKDDNRKLLFNIISGTSMACP 540
Query: 541 HATAVAAYVKSFHPSWSPAALKSALITTAFPMRGDLYPEAEFAYGSGHINPLGAVNPGLI 600
HATAVAAYVKSFHPSWSPAALKSAL+TTAFPMR +L +AEFAYGSGHINPLGAVNPGLI
Sbjct: 541 HATAVAAYVKSFHPSWSPAALKSALMTTAFPMRAELNKDAEFAYGSGHINPLGAVNPGLI 600
Query: 601 YNASEIDYIRFLCGEGYNTTLLRIITKDNSTCSPTNSGLVYDLNYPSFALFTYISTPFSQ 660
YNASEIDYIRFLCGEGYNTTLL+ I +DNSTCSPTNS V+DLNYPSFAL T+IST F+Q
Sbjct: 601 YNASEIDYIRFLCGEGYNTTLLQRIFEDNSTCSPTNSDQVFDLNYPSFALSTHISTSFNQ 660
Query: 661 VYKRKVTNVGSTNSTYKATIFAPSELNITVNPSTLSFKALEEELKFEVTIEGKINGSIAS 720
YKR+VTNVGSTNSTYKAT FAPS +NI+VNPS LSFKAL EELKFE+ IEGKI+ SI S
Sbjct: 661 TYKRRVTNVGSTNSTYKATTFAPSGINISVNPSILSFKALGEELKFELIIEGKISRSIES 720
Query: 721 ASLVWDDGVHKVRSPIIVFDSDTF 745
ASLVWDDGVHKVRSPIIVFDSD F
Sbjct: 721 ASLVWDDGVHKVRSPIIVFDSDPF 738
BLAST of Cmc09g0259781 vs. ExPASy Swiss-Prot
Match:
Q39547 (Cucumisin OS=Cucumis melo OX=3656 PE=1 SV=1)
HSP 1 Score: 807.7 bits (2085), Expect = 1.1e-232
Identity = 422/739 (57.10%), Postives = 519/739 (70.23%), Query Frame = 0
Query: 5 SRLLFLCFCFSLLFFSSLS---EEDDQYRKTYIVYMGSHPKDQVSSTPLSSH-HHMRLLQ 64
S L+F F FSL F + L+ + DD + YIVYMG +D P S+H HH +L+
Sbjct: 3 SSLIFKLFFFSLFFSNRLASRLDSDDDGKNIYIVYMGRKLED-----PDSAHLHHRAMLE 62
Query: 65 EAIGSTFAPHCLLHSYKRSFNGFVAKLTETEAKKVSEMEGVISVFPNEELQLHTTRSWDF 124
+ +GSTFAP +LH+YKRSFNGF KLTE EA+K++ MEGV+SVF NE +LHTTRSWDF
Sbjct: 63 QVVGSTFAPESVLHTYKRSFNGFAVKLTEEEAEKIASMEGVVSVFLNEMNELHTTRSWDF 122
Query: 125 MGFSEQVERVPSVESDIIVGVFDTGIWPESPSFLDHGYGPPPPKWKGSCEVSLNFSCNNK 184
+GF V R VES+I+VGV DTGIWPESPSF D G+ PPPPKWKG+CE S NF CN K
Sbjct: 123 LGFPLTVPRRSQVESNIVVGVLDTGIWPESPSFDDEGFSPPPPKWKGTCETSNNFRCNRK 182
Query: 185 IIGAQSYRSDGRYPIDDIKGPRDSNGHGTHAASTVAGGLVRQASMLGLGTGTARGGVPSA 244
IIGA+SY D+ GPRD+NGHGTH AST AGGLV QA++ GLG GTARGGVP A
Sbjct: 183 IIGARSYHIGRPISPGDVNGPRDTNGHGTHTASTAAGGLVSQANLYGLGLGTARGGVPLA 242
Query: 245 RIAAYKVCWSDTCSGADVLAAFDDAIADGVDIISLSVGPKRPIPNYFNDPIAIGTFHAMR 304
RIAAYKVCW+D CS D+LAA+DDAIADGVDIISLSVG P +YF D IAIG+FHA+
Sbjct: 243 RIAAYKVCWNDGCSDTDILAAYDDAIADGVDIISLSVGGANP-RHYFVDAIAIGSFHAVE 302
Query: 305 NGILTSSSAGNEGPRPFTLTNFSPWALSVAASTTDRRFLTAVQLGDGRKFNGVTINTFDL 364
GILTS+SAGN GP FT + SPW LSVAAST DR+F+T VQ+G+G+ F GV+INTFD
Sbjct: 303 RGILTSNSAGNGGPNFFTTASLSPWLLSVAASTMDRKFVTQVQIGNGQSFQGVSINTFD- 362
Query: 365 NGTQYPLVHAGNIPNVTGGFNGSISRFCLGNSVDRELVKGKIAVCDSIVLPSD-VGSLES 424
YPLV +IPN GF+ S SRFC SV+ L+KGKI VC++ P + SL+
Sbjct: 363 -NQYYPLVSGRDIPNT--GFDKSTSRFCTDKSVNPNLLKGKIVVCEASFGPHEFFKSLDG 422
Query: 425 ALGIIMQDGSPKDLTAAFPLPASHLGTQQRPLISSYLNLTRIPTATILKSTELKLEAAPL 484
A G++M + +D ++PLP+S L Y+ R P ATI KST + +AP+
Sbjct: 423 AAGVLMTSNT-RDYADSYPLPSSVLDPNDLLATLRYIYSIRSPGATIFKSTTILNASAPV 482
Query: 485 VASFSSRGPNPTTPDILKPDVIGPGVEILAAWSPIRSPSDAKGDNRKLLFNIISGTSMAC 544
V SFSSRGPN T D++KPD+ GPGVEILAAW S + G R LFNIISGTSM+C
Sbjct: 483 VVSFSSRGPNRATKDVIKPDISGPGVEILAAWP---SVAPVGGIRRNTLFNIISGTSMSC 542
Query: 545 PHATAVAAYVKSFHPSWSPAALKSALITTAFPMRGDLYPEAEFAYGSGHINPLGAVNPGL 604
PH T +A YVK+++P+WSPAA+KSAL+TTA PM P+AEFAYGSGH+NPL AV PGL
Sbjct: 543 PHITGIATYVKTYNPTWSPAAIKSALMTTASPMNARFNPQAEFAYGSGHVNPLKAVRPGL 602
Query: 605 IYNASEIDYIRFLCGEGYNTTLLRIITKDNSTCSPTNSGLVYDLNYPSFALFTYISTPFS 664
+Y+A+E DY++FLCG+GYNT +R IT D S C+ N+G V+DLNYPSF L S F+
Sbjct: 603 VYDANESDYVKFLCGQGYNTQAVRRITGDYSACTSGNTGRVWDLNYPSFGLSVSPSQTFN 662
Query: 665 QVYKRKVTNVGSTNSTYKATIFAPSELNITVNPSTLSFKALEEELKFEVTIEGKINGSIA 724
Q + R +T+V STY+A I AP L I+VNP+ LSF L + F +T+ G I G +
Sbjct: 663 QYFNRTLTSVAPQASTYRAMISAPQGLTISVNPNVLSFNGLGDRKSFTLTVRGSIKGFVV 722
Query: 725 SASLVWDDGVHKVRSPIIV 739
SASLVW DGVH VRSPI +
Sbjct: 723 SASLVWSDGVHYVRSPITI 727
BLAST of Cmc09g0259781 vs. ExPASy Swiss-Prot
Match:
Q9FIF8 (Subtilisin-like protease SBT4.3 OS=Arabidopsis thaliana OX=3702 GN=SBT4.3 PE=3 SV=1)
HSP 1 Score: 677.9 bits (1748), Expect = 1.3e-193
Identity = 368/745 (49.40%), Postives = 482/745 (64.70%), Query Frame = 0
Query: 1 MSSLSRLLFLCFCFSLLFFSSLSEED-DQYRKTYIVYMGSHPKDQVSSTPLSSHHHMRLL 60
M+ LS L+L C + +F +S D Q YIVYMG+ P ++ +P S HH+ +L
Sbjct: 1 MAKLSTPLYL-ICLAFIFTRDVSANDYRQASSVYIVYMGTLP--EIKYSPPS--HHLSIL 60
Query: 61 QEAIGSTFAPHCLLHSYKRSFNGFVAKLTETEAKKVSEMEGVISVFPNEELQLHTTRSWD 120
Q+ +G+ A H L+ SYKRSFNGF A L++ E++K+ M+ V+SVFP++ +L TTRSWD
Sbjct: 61 QKLVGTIAASHLLVRSYKRSFNGFAANLSQAESQKLQNMKEVVSVFPSKSHELTTTRSWD 120
Query: 121 FMGFSEQVERVPSVESDIIVGVFDTGIWPESPSFLDHGYGPPPPKWKGSCEVSLNFSCNN 180
F+GF E+ R ESD+IVGV D+GIWPES SF D G+GPPP KWKGSC+ L F+CNN
Sbjct: 121 FVGFGEKARRESVKESDVIVGVIDSGIWPESESFDDEGFGPPPKKWKGSCKGGLKFACNN 180
Query: 181 KIIGAQSYRSDGRYPIDDIKGPRDSNGHGTHAASTVAGGLVRQASMLGLGTGTARGGVPS 240
K+IGA+ Y RD GHGTH AST AG V+ AS GL GTARGGVPS
Sbjct: 181 KLIGARFYNKFA-------DSARDEEGHGTHTASTAAGNAVQAASFYGLAQGTARGGVPS 240
Query: 241 ARIAAYKVCWSDTCSGADVLAAFDDAIADGVDIISLSVGPKRPIPNYFNDPIAIGTFHAM 300
ARIAAYKVC+ + C+ D+LAAFDDAIADGVD+IS+S+ + N N +AIG+FHAM
Sbjct: 241 ARIAAYKVCF-NRCNDVDILAAFDDAIADGVDVISISISADY-VSNLLNASVAIGSFHAM 300
Query: 301 RNGILTSSSAGNEGPRPFTLTNFSPWALSVAASTTDRRFLTAVQLGDGRKFNGVTINTFD 360
GI+T+ SAGN GP ++ N SPW ++VAAS TDR+F+ V LG+G+ G+++NTF+
Sbjct: 301 MRGIITAGSAGNNGPDQGSVANVSPWMITVAASGTDRQFIDRVVLGNGKALTGISVNTFN 360
Query: 361 LNGTQYPLVHAGNIPNVTGGFNGSISRFCLGNSVDRELVKGKIAVCDSIVLPSDVGSLES 420
LNGT++P+V+ NV+ + + + +C VD ELVKGKI +CD L L
Sbjct: 361 LNGTKFPIVYG---QNVSRNCSQAQAGYCSSGCVDSELVKGKIVLCDDF-LGYREAYLAG 420
Query: 421 ALGIIMQDGSPKDLTAAFPLPASHLGTQQRPLISSYLNLTRIPTATILKSTELKLEAAPL 480
A+G+I+Q+ D P PAS LG + I SY+ P A IL++ E+ AP
Sbjct: 421 AIGVIVQNTLLPDSAFVVPFPASSLGFEDYKSIKSYIESAEPPQAEILRTEEIVDREAPY 480
Query: 481 VASFSSRGPNPTTPDILKPDVIGPGVEILAAWSPIRSPSDAKG--DNRKLLFNIISGTSM 540
V SFSSRGP+ ++LKPDV PG+EILAA+SP+ SPS D R + ++++SGTSM
Sbjct: 481 VPSFSSRGPSFVIQNLLKPDVSAPGLEILAAFSPVASPSSFLNPEDKRSVRYSVMSGTSM 540
Query: 541 ACPHATAVAAYVKSFHPSWSPAALKSALITTAFPMRGDLYPEAEFAYGSGHINPLGAVNP 600
ACPH VAAYVKSFHP WSP+A+KSA++TTA PM PE EFAYGSG INP A +P
Sbjct: 541 ACPHVAGVAAYVKSFHPDWSPSAIKSAIMTTATPMNLKKNPEQEFAYGSGQINPTKASDP 600
Query: 601 GLIYNASEIDYIRFLCGEGYNTTLLRIITKDNSTCSPTNSGLVYDLNYPSFALFTYISTP 660
GL+Y DY++ LC EG+++T L + N TCS V DLNYP+ F P
Sbjct: 601 GLVYEVETEDYLKMLCAEGFDSTTLTTTSGQNVTCSERTE--VKDLNYPTMTTFVSSLDP 660
Query: 661 FSQVYKRKVTNVGSTNSTYKATIF-APSELNITVNPSTLSFKALEEELKFEVTIEGK--I 720
F+ +KR VTNVG NSTYKA++ EL I++ P L F LEE+ F VTI GK
Sbjct: 661 FNVTFKRTVTNVGFPNSTYKASVVPLQPELQISIEPEILRFGFLEEKKSFVVTISGKELK 720
Query: 721 NGSIASASLVWDDGVHKVRSPIIVF 740
+GS S+S+VW DG H VRSPI+ +
Sbjct: 721 DGSFVSSSVVWSDGSHSVRSPIVAY 725
BLAST of Cmc09g0259781 vs. ExPASy Swiss-Prot
Match:
Q9FGU3 (Subtilisin-like protease SBT4.4 OS=Arabidopsis thaliana OX=3702 GN=SBT4.4 PE=2 SV=1)
HSP 1 Score: 660.2 bits (1702), Expect = 2.7e-188
Identity = 358/750 (47.73%), Postives = 496/750 (66.13%), Query Frame = 0
Query: 1 MSSLSRLLFLCFCFSLLFFSSLSEEDDQY--RKTYIVYMGSHPKDQVSSTPLSSHHHMRL 60
M+ + +FL +L SS+S + D + ++ YIVY+GS P + TP+S HM +
Sbjct: 1 MAKGTTFIFLFSSLLVLSLSSVSADKDDHGDQQVYIVYLGSLPSRE-EYTPMSD--HMSI 60
Query: 61 LQEAIGSTFAPHCLLHSYKRSFNGFVAKLTETEAKKVSEMEGVISVFPNEELQLHTTRSW 120
LQE G + + L+ SYK+SFNGF A+LTE+E K+++ ME V+SVFP+ +L+L TT SW
Sbjct: 61 LQEITGESLIENRLVRSYKKSFNGFAARLTESERKRLAGMERVVSVFPSRKLKLQTTSSW 120
Query: 121 DFMGFSE--QVERVPSVESDIIVGVFDTGIWPESPSFLDHGYGPPPPKWKGSCEVSLNFS 180
+FMG E + +R S+ESD I+GV D+GI+PES SF D G+GPPP KWKG+C NF+
Sbjct: 121 NFMGLKEGIKTKRTRSIESDTIIGVIDSGIYPESDSFSDQGFGPPPKKWKGTCAGGKNFT 180
Query: 181 CNNKIIGAQSYRSDGRYPIDDIKGPRDSNGHGTHAASTVAGGLVRQASMLGLGTGTARGG 240
CNNK+IGA+ Y + + + RD +GHGTH AS AG V ++ GLG GTARGG
Sbjct: 181 CNNKVIGARDYTAKSKAN----QTARDYSGHGTHTASIAAGNAVANSNFYGLGNGTARGG 240
Query: 241 VPSARIAAYKVCWSDTCSGADVLAAFDDAIADGVDIISLSVGPKRPIPNYFNDPIAIGTF 300
VP+ARIA YKVC ++ C G +++AFDDAIADGVD+IS+S+ IP + DPIAIG F
Sbjct: 241 VPAARIAVYKVCDNEGCDGEAMMSAFDDAIADGVDVISISIVLDN-IPPFEEDPIAIGAF 300
Query: 301 HAMRNGILTSSSAGNEGPRPFTLTNFSPWALSVAASTTDRRFLTAVQLGDGRKFNGVTIN 360
HAM G+LT ++AGN GP+ T+T+ +PW SVAAS T+R F+ V LGDG+ G ++N
Sbjct: 301 HAMAVGVLTVNAAGNNGPKISTVTSTAPWVFSVAASVTNRAFMAKVVLGDGKILIGRSVN 360
Query: 361 TFDLNGTQYPLVHAGNIPNVTGGFNGSISRFCLGNSVDRELVKGKIAVCDSIVLPSDVGS 420
T+D+NGT YPLV+ + T + +R C +D +LVKGKI +CDS +
Sbjct: 361 TYDMNGTNYPLVYGKSAALSTCSVDK--ARLCEPKCLDGKLVKGKIVLCDSTKGLIEAQK 420
Query: 421 LESALGIIMQDGSPKDLTAAFPLPASHLGTQQRPLISSYLNLTRIPTATILKSTELKLEA 480
L A+G I+++ P D P S L + SY+N T+ P AT+LKS E+ +
Sbjct: 421 L-GAVGSIVKNPEP-DRAFIRSFPVSFLSNDDYKSLVSYMNSTKNPKATVLKSEEISNQR 480
Query: 481 APLVASFSSRGPNPTTPDILKPDVIGPGVEILAAWSPIRSPSDAKGDNRKLLFNIISGTS 540
APLVASFSSRGP+ DILKPD+ PGVEILAA+SP SP++++ D R++ ++++SGTS
Sbjct: 481 APLVASFSSRGPSSIVSDILKPDITAPGVEILAAYSPDSSPTESEFDTRRVKYSVLSGTS 540
Query: 541 MACPHATAVAAYVKSFHPSWSPAALKSALITTAFPMR--GDLYPEAEFAYGSGHINPLGA 600
MACPH VAAYVK+FHP WSP+ ++SA++TTA+PM G + EFAYGSGH++P+ A
Sbjct: 541 MACPHVAGVAAYVKTFHPQWSPSMIQSAIMTTAWPMNASGSGFVSTEFAYGSGHVDPIDA 600
Query: 601 VNPGLIYNASEIDYIRFLCGEGYNTTLLRIITKDNSTCS-PTNSGLVYDLNYPSFALFTY 660
+NPGL+Y ++ D+I FLCG Y + LRII+ DNSTC+ + L +LNYP+ +
Sbjct: 601 INPGLVYELTKADHINFLCGLNYTSDHLRIISGDNSTCTKEISKTLPRNLNYPTMSAKVS 660
Query: 661 ISTPFSQVYKRKVTNVGSTNSTYKATI--FAPSELNITVNPSTLSFKALEEELKFEVTIE 720
+ PF+ ++R VTNVG STY A + F S+L+I V+P LS K++ E+ F VT+
Sbjct: 661 GTKPFNITFQRTVTNVGMQKSTYNAKVVKFPGSKLSIKVSPRVLSMKSMNEKQSFMVTVS 720
Query: 721 GKINGS--IASASLVWDDGVHKVRSPIIVF 740
G+ SA+L+W DG H VRSPIIV+
Sbjct: 721 SDSIGTKQPVSANLIWSDGTHNVRSPIIVY 738
BLAST of Cmc09g0259781 vs. ExPASy Swiss-Prot
Match:
Q9FIG2 (Subtilisin-like protease SBT4.13 OS=Arabidopsis thaliana OX=3702 GN=SBT4.13 PE=2 SV=1)
HSP 1 Score: 654.1 bits (1686), Expect = 2.0e-186
Identity = 357/753 (47.41%), Postives = 491/753 (65.21%), Query Frame = 0
Query: 1 MSSLSRLLFLCFCFSLLFFSSLSEEDDQYRKTYIVYMGSHPKDQVSSTPLSSHHHMRLLQ 60
M++L+ L C +LF SS+S D ++ YIVYMGS + TP S HM +LQ
Sbjct: 1 MATLAASSSLLSCLLVLFLSSVSAVTDD-KQVYIVYMGS-LSSRADYTPTSD--HMNILQ 60
Query: 61 EAIGSTFAPHCLLHSYKRSFNGFVAKLTETEAKKVSEMEGVISVFPNEELQLHTTRSWDF 120
E G + L+ SYKRSFNGF A+LTE+E ++V++M GV+SVFPN++LQL TT SWDF
Sbjct: 61 EVTGESSIEGRLVRSYKRSFNGFAARLTESERERVAKMVGVVSVFPNKKLQLQTTTSWDF 120
Query: 121 MGFSE--QVERVPSVESDIIVGVFDTGIWPESPSFLDHGYGPPPPKWKGSCEVSLNFSCN 180
MG E + +R P+VESD I+GV D+GI PES SF D G+GPPP KWKG C NF+CN
Sbjct: 121 MGLKEGIKTKRNPTVESDTIIGVIDSGITPESQSFSDKGFGPPPQKWKGVCSGGKNFTCN 180
Query: 181 NKIIGAQSYRSDGRYPIDDIKGPRDSNGHGTHAASTVAGGLVRQASMLGLGTGTARGGVP 240
NK+IGA+ Y S+ G RD +GHGTH AST AG V AS G+G GT RGGVP
Sbjct: 181 NKLIGARDYTSE---------GTRDMDGHGTHTASTAAGNAVVDASFFGIGNGTVRGGVP 240
Query: 241 SARIAAYKVCWSDTCSGADVLAAFDDAIADGVDIISLSVGPKRPIPNYFNDPIAIGTFHA 300
++R+AAYKVC CS +L+AFDDAIADGVD+I++S+G K + NDPIAIG FHA
Sbjct: 241 ASRVAAYKVCTPTGCSSEALLSAFDDAIADGVDLITISIGDK-TASMFQNDPIAIGAFHA 300
Query: 301 MRNGILTSSSAGNEGPRPFTLTNFSPWALSVAASTTDRRFLTAVQLGDGRKFNGVTINTF 360
M G+LT +SAGN GP+P +++ +PW L+VAASTT+R F+T V LG+G+ G ++N +
Sbjct: 301 MAKGVLTVNSAGNSGPKPISVSGVAPWILTVAASTTNRGFVTKVVLGNGKTLVGKSVNAY 360
Query: 361 DLNGTQYPLVHAGNIPNVTGGFNGSISRFCLGNSVDRELVKGKIAVCDSIVLPSDVGSLE 420
++ G YPLV+ + + + + C + VD+ VKGKI VC P + +E
Sbjct: 361 EMKGKDYPLVYGKSA--ASSACDAESAGLCELSCVDKSRVKGKILVCGG---PGGLKIVE 420
Query: 421 S--ALGIIMQDGSPKDLTAAFPLPASHLGTQQRPLISSYLNLTRIPTATILKSTELKLEA 480
S A+G+I + P D+ PLPA+ L T+ + SYL T P A +LK+ +
Sbjct: 421 SVGAVGLIYRTPKP-DVAFIHPLPAAGLLTEDFESLVSYLESTDSPQAIVLKTEAIFNRT 480
Query: 481 APLVASFSSRGPNPTTPDILKPDVIGPGVEILAAWSPIRSPSDAKGDNRKLLFNIISGTS 540
+P++ASFSSRGPN DILKPD+ PGVEILAA+SP PS + D R + ++++SGTS
Sbjct: 481 SPVIASFSSRGPNTIAVDILKPDITAPGVEILAAYSPAGEPS--QDDTRHVKYSVLSGTS 540
Query: 541 MACPHATAVAAYVKSFHPSWSPAALKSALITTAFPMR--GDLYPEAEFAYGSGHINPLGA 600
M+CPH VAAYVK+F+P WSP+ ++SA++TTA+P+ G EFAYGSGH++P+ A
Sbjct: 541 MSCPHVAGVAAYVKTFNPKWSPSMIQSAIMTTAWPVNATGTGIASTEFAYGSGHVDPIAA 600
Query: 601 VNPGLIYNASEIDYIRFLCGEGYNTTLLRIITKDNSTCSPTNSGLVYDLNYPSF-ALFTY 660
NPGL+Y + D+I FLCG Y + +L++I+ + TCS L +LNYPS A +
Sbjct: 601 SNPGLVYELDKSDHIAFLCGMNYTSQVLKVISGETVTCSEAKKILPRNLNYPSMSAKLSG 660
Query: 661 ISTPFSQVYKRKVTNVGSTNSTYKATIFA--PSELNITVNPSTLSFKALEEELKFEVTIE 720
T F+ + R +TNVG+ NSTY + + A S+L++ + PS LSFK + E+ F VT+
Sbjct: 661 SGTTFTVTFNRTLTNVGTPNSTYTSKVVAGHGSKLDVKITPSVLSFKTVNEKQSFTVTVT 720
Query: 721 G-KINGSI-ASASLVWDDGVHKVRSPIIVFDSD 743
G ++ + +SA+L+W DG H VRSPI+V+ SD
Sbjct: 721 GSNLDSEVPSSANLIWSDGTHNVRSPIVVYTSD 731
BLAST of Cmc09g0259781 vs. ExPASy Swiss-Prot
Match:
Q8L7D2 (Subtilisin-like protease SBT4.12 OS=Arabidopsis thaliana OX=3702 GN=SBT4.12 PE=2 SV=1)
HSP 1 Score: 644.8 bits (1662), Expect = 1.2e-183
Identity = 353/749 (47.13%), Postives = 482/749 (64.35%), Query Frame = 0
Query: 1 MSSLSRLLFLCFCFSLLFFSSLSEEDDQYRKTYIVYMGSHPKDQVSSTPLSSHHHMRLLQ 60
M++L+ L +L SS+S D+ + YIVYMGS + P S HM +LQ
Sbjct: 1 MANLAASTCLYSWLLVLLLSSVSAIIDEDTQVYIVYMGS-LSSRADYIPTSD--HMSILQ 60
Query: 61 EAIGSTFAPHCLLHSYKRSFNGFVAKLTETEAKKVSEMEGVISVFPNEELQLHTTRSWDF 120
+ G + L+ SYKRSFNGF A+LTE+E ++E+EGV+SVFPN+ LQLHTT SWDF
Sbjct: 61 QVTGESSIEGRLVRSYKRSFNGFAARLTESERTLIAEIEGVVSVFPNKILQLHTTTSWDF 120
Query: 121 MGFSE--QVERVPSVESDIIVGVFDTGIWPESPSFLDHGYGPPPPKWKGSCEVSLNFSCN 180
MG E +R ++ESD I+GV DTGIWPES SF D G+GPPP KWKG C NF+CN
Sbjct: 121 MGVKEGKNTKRNLAIESDTIIGVIDTGIWPESKSFSDKGFGPPPKKWKGVCSGGKNFTCN 180
Query: 181 NKIIGAQSYRSDGRYPIDDIKGPRDSNGHGTHAASTVAGGLVRQASMLGLGTGTARGGVP 240
NK+IGA+ Y S+ G RD++GHGTH AST AG V+ S G+G GT RGGVP
Sbjct: 181 NKLIGARDYTSE---------GTRDTSGHGTHTASTAAGNAVKDTSFFGIGNGTVRGGVP 240
Query: 241 SARIAAYKVCWSDTCSGADVLAAFDDAIADGVDIISLSVGPKRPIPNYFNDPIAIGTFHA 300
++RIAAYKVC CS +L++FDDAIADGVD+I++S+G + P + +DPIAIG FHA
Sbjct: 241 ASRIAAYKVCTDSGCSSEALLSSFDDAIADGVDLITISIGFQFP-SIFEDDPIAIGAFHA 300
Query: 301 MRNGILTSSSAGNEGPRPFTLTNFSPWALSVAASTTDRRFLTAVQLGDGRKFNGVTINTF 360
M GILT SSAGN GP+P T+++ +PW +VAASTT+R F+T V LG+G+ G ++N F
Sbjct: 301 MAKGILTVSSAGNSGPKPTTVSHVAPWIFTVAASTTNRGFITKVVLGNGKTLAGRSVNAF 360
Query: 361 DLNGTQYPLVHAGNIPNVTGGFNGSISRFCLGNSVDRELVKGKIAVCDSIVLPSDVGSLE 420
D+ G +YPLV+ + + + + C +++ VKGKI VC PS +
Sbjct: 361 DMKGKKYPLVYGKSA--ASSACDAKTAALCAPACLNKSRVKGKILVCGG---PSGYKIAK 420
Query: 421 SALGIIMQDGSPK-DLTAAFPLPASHLGTQQRPLISSYLNLTRIPTATILKSTELKLEAA 480
S I + D SP+ D+ LPAS L + + SY+ P A +LK+ + +
Sbjct: 421 SVGAIAIIDKSPRPDVAFTHHLPASGLKAKDFKSLVSYIESQDSPQAAVLKTETIFNRTS 480
Query: 481 PLVASFSSRGPNPTTPDILKPDVIGPGVEILAAWSPIRSPSDAKGDNRKLLFNIISGTSM 540
P++ASFSSRGPN DILKPD+ PGVEILAA+SP PS+ D R++ +++ SGTSM
Sbjct: 481 PVIASFSSRGPNTIAVDILKPDITAPGVEILAAFSPNGEPSE--DDTRRVKYSVFSGTSM 540
Query: 541 ACPHATAVAAYVKSFHPSWSPAALKSALITTAFPM--RGDLYPEAEFAYGSGHINPLGAV 600
ACPH VAAYVK+F+P WSP+ ++SA++TTA+P+ +G EFAYG+GH++P+ A+
Sbjct: 541 ACPHVAGVAAYVKTFYPRWSPSMIQSAIMTTAWPVKAKGRGIASTEFAYGAGHVDPMAAL 600
Query: 601 NPGLIYNASEIDYIRFLCGEGYNTTLLRIITKDNSTCSPTNSGLVYDLNYPSF-ALFTYI 660
NPGL+Y + D+I FLCG Y + L+II+ D CS N L +LNYPS A +
Sbjct: 601 NPGLVYELDKADHIAFLCGMNYTSKTLKIISGDTVKCSKKNKILPRNLNYPSMSAKLSGT 660
Query: 661 STPFSQVYKRKVTNVGSTNSTYKATIFA--PSELNITVNPSTLSFKALEEELKFEVTIEG 720
+ FS + R +TNVG+ NSTYK+ + A S+L+I V PS L FK + E+ F VT+ G
Sbjct: 661 DSTFSVTFNRTLTNVGTPNSTYKSKVVAGHGSKLSIKVTPSVLYFKTVNEKQSFSVTVTG 720
Query: 721 K-INGSI-ASASLVWDDGVHKVRSPIIVF 740
++ + +SA+L+W DG H VRSPI+V+
Sbjct: 721 SDVDSEVPSSANLIWSDGTHNVRSPIVVY 729
BLAST of Cmc09g0259781 vs. ExPASy TrEMBL
Match:
A0A1S3AUC4 (cucumisin-like isoform X1 OS=Cucumis melo OX=3656 GN=LOC103482910 PE=3 SV=1)
HSP 1 Score: 1493.0 bits (3864), Expect = 0.0e+00
Identity = 744/746 (99.73%), Postives = 745/746 (99.87%), Query Frame = 0
Query: 1 MSSLSRLLFLCFCFSLLFFSSLSEEDDQYRKTYIVYMGSHPKDQVSSTPLSSHHHMRLLQ 60
MSSLSRLLFLCFCFSLLFFSSLSEEDDQYRKTYIVYMGSHPKDQVSSTPLSSHHHMRLLQ
Sbjct: 1 MSSLSRLLFLCFCFSLLFFSSLSEEDDQYRKTYIVYMGSHPKDQVSSTPLSSHHHMRLLQ 60
Query: 61 EAIGSTFAPHCLLHSYKRSFNGFVAKLTETEAKKVSEMEGVISVFPNEELQLHTTRSWDF 120
EAIGSTFAPHCLLHSYKRSFNGFVAKLTETEAKKVSEMEGVISVFPNEELQLHTTRSWDF
Sbjct: 61 EAIGSTFAPHCLLHSYKRSFNGFVAKLTETEAKKVSEMEGVISVFPNEELQLHTTRSWDF 120
Query: 121 MGFSEQVERVPSVESDIIVGVFDTGIWPESPSFLDHGYGPPPPKWKGSCEVSLNFSCNNK 180
MGFSEQVERVPSVESDIIVGVFDTGIWPESPSFLDHGYGPPPPKWKGSCEVSLNFSCNNK
Sbjct: 121 MGFSEQVERVPSVESDIIVGVFDTGIWPESPSFLDHGYGPPPPKWKGSCEVSLNFSCNNK 180
Query: 181 IIGAQSYRSDGRYPIDDIKGPRDSNGHGTHAASTVAGGLVRQASMLGLGTGTARGGVPSA 240
IIGAQSYRSDGRYPIDDIKGPRDSNGHGTHAASTVAGGLVRQASMLGLGTGTARGGVPSA
Sbjct: 181 IIGAQSYRSDGRYPIDDIKGPRDSNGHGTHAASTVAGGLVRQASMLGLGTGTARGGVPSA 240
Query: 241 RIAAYKVCWSDTCSGADVLAAFDDAIADGVDIISLSVGPKRPIPNYFNDPIAIGTFHAMR 300
RIAAYKVCWSDTCSGADVLAAFDDAIADGVDIISLSVGPKRPIPNYFNDPIAIGTFHAMR
Sbjct: 241 RIAAYKVCWSDTCSGADVLAAFDDAIADGVDIISLSVGPKRPIPNYFNDPIAIGTFHAMR 300
Query: 301 NGILTSSSAGNEGPRPFTLTNFSPWALSVAASTTDRRFLTAVQLGDGRKFNGVTINTFDL 360
NGILTSSSAGNEGPRPFTLTNFSPWALSVAASTTDRRFLTAVQLGDGRKFNGVTINTFDL
Sbjct: 301 NGILTSSSAGNEGPRPFTLTNFSPWALSVAASTTDRRFLTAVQLGDGRKFNGVTINTFDL 360
Query: 361 NGTQYPLVHAGNIPNVTGGFNGSISRFCLGNSVDRELVKGKIAVCDSIVLPSDVGSLESA 420
NGTQYPLVHAGNIPNVTGGFNGSISRFCLGNSVDRELVKGKIAVCDSIVLPSDVGSLESA
Sbjct: 361 NGTQYPLVHAGNIPNVTGGFNGSISRFCLGNSVDRELVKGKIAVCDSIVLPSDVGSLESA 420
Query: 421 LGIIMQDGSPKDLTAAFPLPASHLGTQQRPLISSYLNLTRIPTATILKSTELKLEAAPLV 480
LGIIMQDGSPKDLTAAFPLPASHLGTQQRPLISSYLNLTRIPTATILKSTELKLEAAPLV
Sbjct: 421 LGIIMQDGSPKDLTAAFPLPASHLGTQQRPLISSYLNLTRIPTATILKSTELKLEAAPLV 480
Query: 481 ASFSSRGPNPTTPDILKPDVIGPGVEILAAWSPIRSPSDAKGDNRKLLFNIISGTSMACP 540
ASFSSRGPNPTTPDILKPDVIGPGVEILAAWSPIRSPSDAKGDNRKLLFNIISGTSMACP
Sbjct: 481 ASFSSRGPNPTTPDILKPDVIGPGVEILAAWSPIRSPSDAKGDNRKLLFNIISGTSMACP 540
Query: 541 HATAVAAYVKSFHPSWSPAALKSALITTAFPMRGDLYPEAEFAYGSGHINPLGAVNPGLI 600
HATAVAAYVKSFHPSWSPAALKSALITTAFPMRGDLYPEAEFAYGSGHINPLGAVNPGLI
Sbjct: 541 HATAVAAYVKSFHPSWSPAALKSALITTAFPMRGDLYPEAEFAYGSGHINPLGAVNPGLI 600
Query: 601 YNASEIDYIRFLCGEGYNTTLLRIITKDNSTCSPTNSGLVYDLNYPSFALFTYISTPFSQ 660
YNASEIDYIRFLCGEGYNTTLLRIITKDNSTCSPTNSGLVYDLNY SFALFTYISTPFSQ
Sbjct: 601 YNASEIDYIRFLCGEGYNTTLLRIITKDNSTCSPTNSGLVYDLNYHSFALFTYISTPFSQ 660
Query: 661 VYKRKVTNVGSTNSTYKATIFAPSELNITVNPSTLSFKALEEELKFEVTIEGKINGSIAS 720
VYKR+VTNVGSTNSTYKATIFAPSELNITVNPSTLSFKALEEELKFEVTIEGKINGSIAS
Sbjct: 661 VYKRRVTNVGSTNSTYKATIFAPSELNITVNPSTLSFKALEEELKFEVTIEGKINGSIAS 720
Query: 721 ASLVWDDGVHKVRSPIIVFDSDTFPR 747
ASLVWDDGVHKVRSPIIVFDSDTFPR
Sbjct: 721 ASLVWDDGVHKVRSPIIVFDSDTFPR 746
BLAST of Cmc09g0259781 vs. ExPASy TrEMBL
Match:
A0A1S3AUU4 (cucumisin-like isoform X2 OS=Cucumis melo OX=3656 GN=LOC103482910 PE=3 SV=1)
HSP 1 Score: 1421.0 bits (3677), Expect = 0.0e+00
Identity = 706/708 (99.72%), Postives = 707/708 (99.86%), Query Frame = 0
Query: 39 SHPKDQVSSTPLSSHHHMRLLQEAIGSTFAPHCLLHSYKRSFNGFVAKLTETEAKKVSEM 98
SHPKDQVSSTPLSSHHHMRLLQEAIGSTFAPHCLLHSYKRSFNGFVAKLTETEAKKVSEM
Sbjct: 6 SHPKDQVSSTPLSSHHHMRLLQEAIGSTFAPHCLLHSYKRSFNGFVAKLTETEAKKVSEM 65
Query: 99 EGVISVFPNEELQLHTTRSWDFMGFSEQVERVPSVESDIIVGVFDTGIWPESPSFLDHGY 158
EGVISVFPNEELQLHTTRSWDFMGFSEQVERVPSVESDIIVGVFDTGIWPESPSFLDHGY
Sbjct: 66 EGVISVFPNEELQLHTTRSWDFMGFSEQVERVPSVESDIIVGVFDTGIWPESPSFLDHGY 125
Query: 159 GPPPPKWKGSCEVSLNFSCNNKIIGAQSYRSDGRYPIDDIKGPRDSNGHGTHAASTVAGG 218
GPPPPKWKGSCEVSLNFSCNNKIIGAQSYRSDGRYPIDDIKGPRDSNGHGTHAASTVAGG
Sbjct: 126 GPPPPKWKGSCEVSLNFSCNNKIIGAQSYRSDGRYPIDDIKGPRDSNGHGTHAASTVAGG 185
Query: 219 LVRQASMLGLGTGTARGGVPSARIAAYKVCWSDTCSGADVLAAFDDAIADGVDIISLSVG 278
LVRQASMLGLGTGTARGGVPSARIAAYKVCWSDTCSGADVLAAFDDAIADGVDIISLSVG
Sbjct: 186 LVRQASMLGLGTGTARGGVPSARIAAYKVCWSDTCSGADVLAAFDDAIADGVDIISLSVG 245
Query: 279 PKRPIPNYFNDPIAIGTFHAMRNGILTSSSAGNEGPRPFTLTNFSPWALSVAASTTDRRF 338
PKRPIPNYFNDPIAIGTFHAMRNGILTSSSAGNEGPRPFTLTNFSPWALSVAASTTDRRF
Sbjct: 246 PKRPIPNYFNDPIAIGTFHAMRNGILTSSSAGNEGPRPFTLTNFSPWALSVAASTTDRRF 305
Query: 339 LTAVQLGDGRKFNGVTINTFDLNGTQYPLVHAGNIPNVTGGFNGSISRFCLGNSVDRELV 398
LTAVQLGDGRKFNGVTINTFDLNGTQYPLVHAGNIPNVTGGFNGSISRFCLGNSVDRELV
Sbjct: 306 LTAVQLGDGRKFNGVTINTFDLNGTQYPLVHAGNIPNVTGGFNGSISRFCLGNSVDRELV 365
Query: 399 KGKIAVCDSIVLPSDVGSLESALGIIMQDGSPKDLTAAFPLPASHLGTQQRPLISSYLNL 458
KGKIAVCDSIVLPSDVGSLESALGIIMQDGSPKDLTAAFPLPASHLGTQQRPLISSYLNL
Sbjct: 366 KGKIAVCDSIVLPSDVGSLESALGIIMQDGSPKDLTAAFPLPASHLGTQQRPLISSYLNL 425
Query: 459 TRIPTATILKSTELKLEAAPLVASFSSRGPNPTTPDILKPDVIGPGVEILAAWSPIRSPS 518
TRIPTATILKSTELKLEAAPLVASFSSRGPNPTTPDILKPDVIGPGVEILAAWSPIRSPS
Sbjct: 426 TRIPTATILKSTELKLEAAPLVASFSSRGPNPTTPDILKPDVIGPGVEILAAWSPIRSPS 485
Query: 519 DAKGDNRKLLFNIISGTSMACPHATAVAAYVKSFHPSWSPAALKSALITTAFPMRGDLYP 578
DAKGDNRKLLFNIISGTSMACPHATAVAAYVKSFHPSWSPAALKSALITTAFPMRGDLYP
Sbjct: 486 DAKGDNRKLLFNIISGTSMACPHATAVAAYVKSFHPSWSPAALKSALITTAFPMRGDLYP 545
Query: 579 EAEFAYGSGHINPLGAVNPGLIYNASEIDYIRFLCGEGYNTTLLRIITKDNSTCSPTNSG 638
EAEFAYGSGHINPLGAVNPGLIYNASEIDYIRFLCGEGYNTTLLRIITKDNSTCSPTNSG
Sbjct: 546 EAEFAYGSGHINPLGAVNPGLIYNASEIDYIRFLCGEGYNTTLLRIITKDNSTCSPTNSG 605
Query: 639 LVYDLNYPSFALFTYISTPFSQVYKRKVTNVGSTNSTYKATIFAPSELNITVNPSTLSFK 698
LVYDLNY SFALFTYISTPFSQVYKR+VTNVGSTNSTYKATIFAPSELNITVNPSTLSFK
Sbjct: 606 LVYDLNYHSFALFTYISTPFSQVYKRRVTNVGSTNSTYKATIFAPSELNITVNPSTLSFK 665
Query: 699 ALEEELKFEVTIEGKINGSIASASLVWDDGVHKVRSPIIVFDSDTFPR 747
ALEEELKFEVTIEGKINGSIASASLVWDDGVHKVRSPIIVFDSDTFPR
Sbjct: 666 ALEEELKFEVTIEGKINGSIASASLVWDDGVHKVRSPIIVFDSDTFPR 713
BLAST of Cmc09g0259781 vs. ExPASy TrEMBL
Match:
A0A1S3AUT1 (cucumisin-like isoform X3 OS=Cucumis melo OX=3656 GN=LOC103482910 PE=3 SV=1)
HSP 1 Score: 1370.1 bits (3545), Expect = 0.0e+00
Identity = 681/684 (99.56%), Postives = 682/684 (99.71%), Query Frame = 0
Query: 63 IGSTFAPHCLLHSYKRSFNGFVAKLTETEAKKVSEMEGVISVFPNEELQLHTTRSWDFMG 122
I STFAPHCLLHSYKRSFNGFVAKLTETEAKKVSEMEGVISVFPNEELQLHTTRSWDFMG
Sbjct: 4 IESTFAPHCLLHSYKRSFNGFVAKLTETEAKKVSEMEGVISVFPNEELQLHTTRSWDFMG 63
Query: 123 FSEQVERVPSVESDIIVGVFDTGIWPESPSFLDHGYGPPPPKWKGSCEVSLNFSCNNKII 182
FSEQVERVPSVESDIIVGVFDTGIWPESPSFLDHGYGPPPPKWKGSCEVSLNFSCNNKII
Sbjct: 64 FSEQVERVPSVESDIIVGVFDTGIWPESPSFLDHGYGPPPPKWKGSCEVSLNFSCNNKII 123
Query: 183 GAQSYRSDGRYPIDDIKGPRDSNGHGTHAASTVAGGLVRQASMLGLGTGTARGGVPSARI 242
GAQSYRSDGRYPIDDIKGPRDSNGHGTHAASTVAGGLVRQASMLGLGTGTARGGVPSARI
Sbjct: 124 GAQSYRSDGRYPIDDIKGPRDSNGHGTHAASTVAGGLVRQASMLGLGTGTARGGVPSARI 183
Query: 243 AAYKVCWSDTCSGADVLAAFDDAIADGVDIISLSVGPKRPIPNYFNDPIAIGTFHAMRNG 302
AAYKVCWSDTCSGADVLAAFDDAIADGVDIISLSVGPKRPIPNYFNDPIAIGTFHAMRNG
Sbjct: 184 AAYKVCWSDTCSGADVLAAFDDAIADGVDIISLSVGPKRPIPNYFNDPIAIGTFHAMRNG 243
Query: 303 ILTSSSAGNEGPRPFTLTNFSPWALSVAASTTDRRFLTAVQLGDGRKFNGVTINTFDLNG 362
ILTSSSAGNEGPRPFTLTNFSPWALSVAASTTDRRFLTAVQLGDGRKFNGVTINTFDLNG
Sbjct: 244 ILTSSSAGNEGPRPFTLTNFSPWALSVAASTTDRRFLTAVQLGDGRKFNGVTINTFDLNG 303
Query: 363 TQYPLVHAGNIPNVTGGFNGSISRFCLGNSVDRELVKGKIAVCDSIVLPSDVGSLESALG 422
TQYPLVHAGNIPNVTGGFNGSISRFCLGNSVDRELVKGKIAVCDSIVLPSDVGSLESALG
Sbjct: 304 TQYPLVHAGNIPNVTGGFNGSISRFCLGNSVDRELVKGKIAVCDSIVLPSDVGSLESALG 363
Query: 423 IIMQDGSPKDLTAAFPLPASHLGTQQRPLISSYLNLTRIPTATILKSTELKLEAAPLVAS 482
IIMQDGSPKDLTAAFPLPASHLGTQQRPLISSYLNLTRIPTATILKSTELKLEAAPLVAS
Sbjct: 364 IIMQDGSPKDLTAAFPLPASHLGTQQRPLISSYLNLTRIPTATILKSTELKLEAAPLVAS 423
Query: 483 FSSRGPNPTTPDILKPDVIGPGVEILAAWSPIRSPSDAKGDNRKLLFNIISGTSMACPHA 542
FSSRGPNPTTPDILKPDVIGPGVEILAAWSPIRSPSDAKGDNRKLLFNIISGTSMACPHA
Sbjct: 424 FSSRGPNPTTPDILKPDVIGPGVEILAAWSPIRSPSDAKGDNRKLLFNIISGTSMACPHA 483
Query: 543 TAVAAYVKSFHPSWSPAALKSALITTAFPMRGDLYPEAEFAYGSGHINPLGAVNPGLIYN 602
TAVAAYVKSFHPSWSPAALKSALITTAFPMRGDLYPEAEFAYGSGHINPLGAVNPGLIYN
Sbjct: 484 TAVAAYVKSFHPSWSPAALKSALITTAFPMRGDLYPEAEFAYGSGHINPLGAVNPGLIYN 543
Query: 603 ASEIDYIRFLCGEGYNTTLLRIITKDNSTCSPTNSGLVYDLNYPSFALFTYISTPFSQVY 662
ASEIDYIRFLCGEGYNTTLLRIITKDNSTCSPTNSGLVYDLNY SFALFTYISTPFSQVY
Sbjct: 544 ASEIDYIRFLCGEGYNTTLLRIITKDNSTCSPTNSGLVYDLNYHSFALFTYISTPFSQVY 603
Query: 663 KRKVTNVGSTNSTYKATIFAPSELNITVNPSTLSFKALEEELKFEVTIEGKINGSIASAS 722
KR+VTNVGSTNSTYKATIFAPSELNITVNPSTLSFKALEEELKFEVTIEGKINGSIASAS
Sbjct: 604 KRRVTNVGSTNSTYKATIFAPSELNITVNPSTLSFKALEEELKFEVTIEGKINGSIASAS 663
Query: 723 LVWDDGVHKVRSPIIVFDSDTFPR 747
LVWDDGVHKVRSPIIVFDSDTFPR
Sbjct: 664 LVWDDGVHKVRSPIIVFDSDTFPR 687
BLAST of Cmc09g0259781 vs. ExPASy TrEMBL
Match:
A0A0A0KJN5 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G141040 PE=3 SV=1)
HSP 1 Score: 1351.3 bits (3496), Expect = 0.0e+00
Identity = 681/746 (91.29%), Postives = 701/746 (93.97%), Query Frame = 0
Query: 1 MSSLSRLLFLCFCFSLLFFSSLSEEDDQYRKTYIVYMGSHPKDQVSSTPLSSHHHMRLLQ 60
MSSLSRLLFLC CFSLLF +S+S EDDQYRKTYIVYMGSH QVSS PLSSHHHMR+LQ
Sbjct: 1 MSSLSRLLFLCLCFSLLFSTSVS-EDDQYRKTYIVYMGSH--HQVSSAPLSSHHHMRILQ 60
Query: 61 EAIGSTFAPHCLLHSYKRSFNGFVAKLTETEAKKVSEMEGVISVFPNEELQLHTTRSWDF 120
EA+GSTFAPHCLLHSYKRSFNGFVAKLTE EAKKVSEMEGVISVFPN ELQLHTTRSWDF
Sbjct: 61 EAVGSTFAPHCLLHSYKRSFNGFVAKLTEIEAKKVSEMEGVISVFPNGELQLHTTRSWDF 120
Query: 121 MGFSEQVERVPSVESDIIVGVFDTGIWPESPSFLDHGYGPPPPKWKGSCEVSLNFSCNNK 180
MG SEQVERVPSVESDIIVGVFDTGIWPESPSFLDHGYGPPPPKWKGSCEVS NFSCNNK
Sbjct: 121 MGMSEQVERVPSVESDIIVGVFDTGIWPESPSFLDHGYGPPPPKWKGSCEVSANFSCNNK 180
Query: 181 IIGAQSYRSDGRYPIDDIKGPRDSNGHGTHAASTVAGGLVRQASMLGLGTGTARGGVPSA 240
IIGA+SYRSDGRYPIDDIKGPRDSNGHGTHAASTVAGGLVRQASMLGLG GTARGGVPSA
Sbjct: 181 IIGARSYRSDGRYPIDDIKGPRDSNGHGTHAASTVAGGLVRQASMLGLGMGTARGGVPSA 240
Query: 241 RIAAYKVCWSDTCSGADVLAAFDDAIADGVDIISLSVGPKRPIPNYFNDPIAIGTFHAMR 300
RIAAYKVCWSDTCS ADVLAAFDDAIADGVDIIS+SVGPKRP PNYF DPIAIGTFHAMR
Sbjct: 241 RIAAYKVCWSDTCSDADVLAAFDDAIADGVDIISMSVGPKRPRPNYFQDPIAIGTFHAMR 300
Query: 301 NGILTSSSAGNEGPRPFTLTNFSPWALSVAASTTDRRFLTAVQLGDGRKFNGVTINTFDL 360
NGILTS+SAGNEGP FT+TNFSPWALSVAAST+DRRFLTAVQLGDGRKFNGVTINTFDL
Sbjct: 301 NGILTSTSAGNEGPLHFTVTNFSPWALSVAASTSDRRFLTAVQLGDGRKFNGVTINTFDL 360
Query: 361 NGTQYPLVHAGNIPNVTGGFNGSISRFCLGNSVDRELVKGKIAVCDSIVLPSDVGSLESA 420
NGTQYPLV+AGNIPNVTGGFNGS SRFCL +SVDRELVKGKIA+CDS V PSDVGSLESA
Sbjct: 361 NGTQYPLVYAGNIPNVTGGFNGSFSRFCLRDSVDRELVKGKIAICDSFVSPSDVGSLESA 420
Query: 421 LGIIMQDGSPKDLTAAFPLPASHLGTQQRPLISSYLNLTRIPTATILKSTELKLEAAPLV 480
+GIIMQD SPKDLT AFPLPASHLG QQRPLISSYLN TRIPTATILKST LKL+ APLV
Sbjct: 421 VGIIMQDRSPKDLTFAFPLPASHLGIQQRPLISSYLNSTRIPTATILKSTGLKLQVAPLV 480
Query: 481 ASFSSRGPNPTTPDILKPDVIGPGVEILAAWSPIRSPSDAKGDNRKLLFNIISGTSMACP 540
ASFSSRGPNPT+P ILKPDVIGPGVEILAAWSP+RSPS+AKGDNRKLLFNIISGTSMACP
Sbjct: 481 ASFSSRGPNPTSPYILKPDVIGPGVEILAAWSPLRSPSNAKGDNRKLLFNIISGTSMACP 540
Query: 541 HATAVAAYVKSFHPSWSPAALKSALITTAFPMRGDLYPEAEFAYGSGHINPLGAVNPGLI 600
HATAVAAYVKSFHPSWSPAALKSALITTAFPMRGDLYPEAEFAYGSGHINPLGAVNPGLI
Sbjct: 541 HATAVAAYVKSFHPSWSPAALKSALITTAFPMRGDLYPEAEFAYGSGHINPLGAVNPGLI 600
Query: 601 YNASEIDYIRFLCGEGYNTTLLRIITKDNSTCSPTNSGLVYDLNYPSFALFTYISTPFSQ 660
YNASE DYIRFLC EGYNTT LRIITKDNSTCS T S VYDLNYPSFALFT+ISTPFSQ
Sbjct: 601 YNASETDYIRFLCDEGYNTTFLRIITKDNSTCSTTQSIRVYDLNYPSFALFTHISTPFSQ 660
Query: 661 VYKRKVTNVGSTNSTYKATIFAPSELNITVNPSTLSFKALEEELKFEVTIEGKINGSIAS 720
KR+VTNVGSTNSTYKATI APS LNITVNPS LSFKALEEEL FEVT EGKI+ SI S
Sbjct: 661 TSKRRVTNVGSTNSTYKATISAPSGLNITVNPSILSFKALEEELNFEVTFEGKIDRSIES 720
Query: 721 ASLVWDDGVHKVRSPIIVFDSDTFPR 747
ASLVWDDGVHKVRSPIIVFDSDTF R
Sbjct: 721 ASLVWDDGVHKVRSPIIVFDSDTFTR 743
BLAST of Cmc09g0259781 vs. ExPASy TrEMBL
Match:
A0A6J1E2G4 (cucumisin-like OS=Cucurbita moschata OX=3662 GN=LOC111430231 PE=3 SV=1)
HSP 1 Score: 1161.7 bits (3004), Expect = 0.0e+00
Identity = 578/744 (77.69%), Postives = 649/744 (87.23%), Query Frame = 0
Query: 1 MSSLSRLLFLCFCFSLLFFSSLSEEDDQYRKTYIVYMGSHPKDQVSSTPLSSHHHMRLLQ 60
MSSLSRLLFL F S LFFSS SE+DD ++TYIVYMGSHPKD+VS + HH+R+LQ
Sbjct: 1 MSSLSRLLFLAFWLSRLFFSSNSEDDD--KRTYIVYMGSHPKDRVS----TRSHHVRMLQ 60
Query: 61 EAIGSTFAPHCLLHSYKRSFNGFVAKLTETEAKKVSEMEGVISVFPNEELQLHTTRSWDF 120
E IGS+FAPH LLHSY+RSFNGFV KLTE E K +SEM+GVISVFPNE+ QLHTTRSWDF
Sbjct: 61 ETIGSSFAPHSLLHSYQRSFNGFVVKLTEDEVKIISEMKGVISVFPNEKKQLHTTRSWDF 120
Query: 121 MGFSEQVERVPSVESDIIVGVFDTGIWPESPSFLDHGYGPPPPKWKGSCEVSLNFSCNNK 180
MG S+QV RVPSVESDIIVGV DTGIWPESPSFLD GYGPPPP+WKGSCE SLNFSCNNK
Sbjct: 121 MGLSQQVGRVPSVESDIIVGVLDTGIWPESPSFLDEGYGPPPPRWKGSCEASLNFSCNNK 180
Query: 181 IIGAQSYRSDGRYPIDDIKGPRDSNGHGTHAASTVAGGLVRQASMLGLGTGTARGGVPSA 240
IIGA+SYR++G+YPI+DI+GPRDSNGHGTH ASTVAGGLVRQASMLGLG+GTARGGVPSA
Sbjct: 181 IIGARSYRTNGQYPINDIQGPRDSNGHGTHTASTVAGGLVRQASMLGLGSGTARGGVPSA 240
Query: 241 RIAAYKVCWSDTCSGADVLAAFDDAIADGVDIISLSVGPKRPIPNYFNDPIAIGTFHAMR 300
RIA+YK+CWSD CS AD+LAAFDDAIADGVDIIS SVG P +YFND +AIG FHAM+
Sbjct: 241 RIASYKICWSDDCSDADILAAFDDAIADGVDIISFSVGGHTP-KDYFNDSMAIGAFHAMK 300
Query: 301 NGILTSSSAGNEGPRPFTLTNFSPWALSVAASTTDRRFLTAVQLGDGRKFNGVTINTFDL 360
GILTS SAGN+GP+ FT+ NFSPW+LSVAASTT+R +L+ +QLGDGR FNGVTINTFDL
Sbjct: 301 KGILTSMSAGNDGPQSFTIRNFSPWSLSVAASTTNRSYLSGIQLGDGRSFNGVTINTFDL 360
Query: 361 NGTQYPLVHAGNIPNVTGGFNGSISRFCLGNSVDRELVKGKIAVCDSIVLPSDVGSLESA 420
NGTQYPLV+AGNIPN+TGGFNGSISRFCL NSVDRE VKGKI +CD V P ++GSLE A
Sbjct: 361 NGTQYPLVYAGNIPNITGGFNGSISRFCLPNSVDRESVKGKIVLCDFFVSPKNLGSLEGA 420
Query: 421 LGIIMQDGSPKDLTAAFPLPASHLGTQQRPLISSYLNLTRIPTATILKSTELKLEAAPLV 480
+GIIMQD +PKDLT FPLPASHLGTQ+ LISSY NLT +PTATILKSTE K +A P V
Sbjct: 421 IGIIMQDTNPKDLTFPFPLPASHLGTQEGALISSYANLTGLPTATILKSTEGKYKATPFV 480
Query: 481 ASFSSRGPNPTTPDILKPDVIGPGVEILAAWSPIRSPSDAKGDNRKLLFNIISGTSMACP 540
ASFSSRGPNP TPDILKPD+ GPGVEILAAWSPI PS A+ D R+LLFNIISGTSM+CP
Sbjct: 481 ASFSSRGPNPITPDILKPDLSGPGVEILAAWSPIGPPSGAEEDTRQLLFNIISGTSMSCP 540
Query: 541 HATAVAAYVKSFHPSWSPAALKSALITTAFPMRGDLYPEAEFAYGSGHINPLGAVNPGLI 600
HATAVAAYVK+FHPSWSPAALKSAL+TTAFPMR DL P+AEFAYGSGHINPL AVNPGLI
Sbjct: 541 HATAVAAYVKTFHPSWSPAALKSALMTTAFPMRADLNPDAEFAYGSGHINPLSAVNPGLI 600
Query: 601 YNASEIDYIRFLCGEGYNTTLLRIITKDNSTCSPTNSGLVYDLNYPSFALFTYISTPFSQ 660
YNA+EIDY+RFLCG+GY+T L++ ++ DNS+CS + LV+DLNYPSFAL T IST SQ
Sbjct: 601 YNATEIDYVRFLCGQGYSTKLVQQVSGDNSSCSRGDIDLVFDLNYPSFALSTSISTSISQ 660
Query: 661 VYKRKVTNVGSTNSTYKATIFAPSELNITVNPSTLSFKALEEELKFEVTIEGKINGSIAS 720
VY+R+VTNVGS NSTYKA + PS LNITVNPS LSFKAL EEL FE+TIEG I+ SIAS
Sbjct: 661 VYRRRVTNVGSANSTYKAIVSGPSGLNITVNPSVLSFKALGEELSFELTIEGSISSSIAS 720
Query: 721 ASLVWDDGVHKVRSPIIVFDSDTF 745
ASLVWDDG HKV+SPI+VFD +TF
Sbjct: 721 ASLVWDDGKHKVKSPIVVFDENTF 737
BLAST of Cmc09g0259781 vs. TAIR 10
Match:
AT5G59190.1 (subtilase family protein )
HSP 1 Score: 664.5 bits (1713), Expect = 1.0e-190
Identity = 354/708 (50.00%), Postives = 463/708 (65.40%), Query Frame = 0
Query: 37 MGSHPKDQVSSTPLSSHHHMRLLQEAIGSTFAPHCLLHSYKRSFNGFVAKLTETEAKKVS 96
MG+ P ++ +P S HH+ +LQ+ +G+ A H L+ SYKRSFNGF A L++ E++K+
Sbjct: 1 MGTLP--EIKYSPPS--HHLSILQKLVGTIAASHLLVRSYKRSFNGFAANLSQAESQKLQ 60
Query: 97 EMEGVISVFPNEELQLHTTRSWDFMGFSEQVERVPSVESDIIVGVFDTGIWPESPSFLDH 156
M+ V+SVFP++ +L TTRSWDF+GF E+ R ESD+IVGV D+GIWPES SF D
Sbjct: 61 NMKEVVSVFPSKSHELTTTRSWDFVGFGEKARRESVKESDVIVGVIDSGIWPESESFDDE 120
Query: 157 GYGPPPPKWKGSCEVSLNFSCNNKIIGAQSYRSDGRYPIDDIKGPRDSNGHGTHAASTVA 216
G+GPPP KWKGSC+ L F+CNNK+IGA+ Y RD GHGTH AST A
Sbjct: 121 GFGPPPKKWKGSCKGGLKFACNNKLIGARFYNKFA-------DSARDEEGHGTHTASTAA 180
Query: 217 GGLVRQASMLGLGTGTARGGVPSARIAAYKVCWSDTCSGADVLAAFDDAIADGVDIISLS 276
G V+ AS GL GTARGGVPSARIAAYKVC+ + C+ D+LAAFDDAIADGVD+IS+S
Sbjct: 181 GNAVQAASFYGLAQGTARGGVPSARIAAYKVCF-NRCNDVDILAAFDDAIADGVDVISIS 240
Query: 277 VGPKRPIPNYFNDPIAIGTFHAMRNGILTSSSAGNEGPRPFTLTNFSPWALSVAASTTDR 336
+ + N N +AIG+FHAM GI+T+ SAGN GP ++ N SPW ++VAAS TDR
Sbjct: 241 ISADY-VSNLLNASVAIGSFHAMMRGIITAGSAGNNGPDQGSVANVSPWMITVAASGTDR 300
Query: 337 RFLTAVQLGDGRKFNGVTINTFDLNGTQYPLVHAGNIPNVTGGFNGSISRFCLGNSVDRE 396
+F+ V LG+G+ G+++NTF+LNGT++P+V+ NV+ + + + +C VD E
Sbjct: 301 QFIDRVVLGNGKALTGISVNTFNLNGTKFPIVYG---QNVSRNCSQAQAGYCSSGCVDSE 360
Query: 397 LVKGKIAVCDSIVLPSDVGSLESALGIIMQDGSPKDLTAAFPLPASHLGTQQRPLISSYL 456
LVKGKI +CD L L A+G+I+Q+ D P PAS LG + I SY+
Sbjct: 361 LVKGKIVLCDDF-LGYREAYLAGAIGVIVQNTLLPDSAFVVPFPASSLGFEDYKSIKSYI 420
Query: 457 NLTRIPTATILKSTELKLEAAPLVASFSSRGPNPTTPDILKPDVIGPGVEILAAWSPIRS 516
P A IL++ E+ AP V SFSSRGP+ ++LKPDV PG+EILAA+SP+ S
Sbjct: 421 ESAEPPQAEILRTEEIVDREAPYVPSFSSRGPSFVIQNLLKPDVSAPGLEILAAFSPVAS 480
Query: 517 PSDAKG--DNRKLLFNIISGTSMACPHATAVAAYVKSFHPSWSPAALKSALITTAFPMRG 576
PS D R + ++++SGTSMACPH VAAYVKSFHP WSP+A+KSA++TTA PM
Sbjct: 481 PSSFLNPEDKRSVRYSVMSGTSMACPHVAGVAAYVKSFHPDWSPSAIKSAIMTTATPMNL 540
Query: 577 DLYPEAEFAYGSGHINPLGAVNPGLIYNASEIDYIRFLCGEGYNTTLLRIITKDNSTCSP 636
PE EFAYGSG INP A +PGL+Y DY++ LC EG+++T L + N TCS
Sbjct: 541 KKNPEQEFAYGSGQINPTKASDPGLVYEVETEDYLKMLCAEGFDSTTLTTTSGQNVTCSE 600
Query: 637 TNSGLVYDLNYPSFALFTYISTPFSQVYKRKVTNVGSTNSTYKATIF-APSELNITVNPS 696
V DLNYP+ F PF+ +KR VTNVG NSTYKA++ EL I++ P
Sbjct: 601 RTE--VKDLNYPTMTTFVSSLDPFNVTFKRTVTNVGFPNSTYKASVVPLQPELQISIEPE 660
Query: 697 TLSFKALEEELKFEVTIEGK--INGSIASASLVWDDGVHKVRSPIIVF 740
L F LEE+ F VTI GK +GS S+S+VW DG H VRSPI+ +
Sbjct: 661 ILRFGFLEEKKSFVVTISGKELKDGSFVSSSVVWSDGSHSVRSPIVAY 689
BLAST of Cmc09g0259781 vs. TAIR 10
Match:
AT5G59100.1 (Subtilisin-like serine endopeptidase family protein )
HSP 1 Score: 660.2 bits (1702), Expect = 1.9e-189
Identity = 358/750 (47.73%), Postives = 496/750 (66.13%), Query Frame = 0
Query: 1 MSSLSRLLFLCFCFSLLFFSSLSEEDDQY--RKTYIVYMGSHPKDQVSSTPLSSHHHMRL 60
M+ + +FL +L SS+S + D + ++ YIVY+GS P + TP+S HM +
Sbjct: 1 MAKGTTFIFLFSSLLVLSLSSVSADKDDHGDQQVYIVYLGSLPSRE-EYTPMSD--HMSI 60
Query: 61 LQEAIGSTFAPHCLLHSYKRSFNGFVAKLTETEAKKVSEMEGVISVFPNEELQLHTTRSW 120
LQE G + + L+ SYK+SFNGF A+LTE+E K+++ ME V+SVFP+ +L+L TT SW
Sbjct: 61 LQEITGESLIENRLVRSYKKSFNGFAARLTESERKRLAGMERVVSVFPSRKLKLQTTSSW 120
Query: 121 DFMGFSE--QVERVPSVESDIIVGVFDTGIWPESPSFLDHGYGPPPPKWKGSCEVSLNFS 180
+FMG E + +R S+ESD I+GV D+GI+PES SF D G+GPPP KWKG+C NF+
Sbjct: 121 NFMGLKEGIKTKRTRSIESDTIIGVIDSGIYPESDSFSDQGFGPPPKKWKGTCAGGKNFT 180
Query: 181 CNNKIIGAQSYRSDGRYPIDDIKGPRDSNGHGTHAASTVAGGLVRQASMLGLGTGTARGG 240
CNNK+IGA+ Y + + + RD +GHGTH AS AG V ++ GLG GTARGG
Sbjct: 181 CNNKVIGARDYTAKSKAN----QTARDYSGHGTHTASIAAGNAVANSNFYGLGNGTARGG 240
Query: 241 VPSARIAAYKVCWSDTCSGADVLAAFDDAIADGVDIISLSVGPKRPIPNYFNDPIAIGTF 300
VP+ARIA YKVC ++ C G +++AFDDAIADGVD+IS+S+ IP + DPIAIG F
Sbjct: 241 VPAARIAVYKVCDNEGCDGEAMMSAFDDAIADGVDVISISIVLDN-IPPFEEDPIAIGAF 300
Query: 301 HAMRNGILTSSSAGNEGPRPFTLTNFSPWALSVAASTTDRRFLTAVQLGDGRKFNGVTIN 360
HAM G+LT ++AGN GP+ T+T+ +PW SVAAS T+R F+ V LGDG+ G ++N
Sbjct: 301 HAMAVGVLTVNAAGNNGPKISTVTSTAPWVFSVAASVTNRAFMAKVVLGDGKILIGRSVN 360
Query: 361 TFDLNGTQYPLVHAGNIPNVTGGFNGSISRFCLGNSVDRELVKGKIAVCDSIVLPSDVGS 420
T+D+NGT YPLV+ + T + +R C +D +LVKGKI +CDS +
Sbjct: 361 TYDMNGTNYPLVYGKSAALSTCSVDK--ARLCEPKCLDGKLVKGKIVLCDSTKGLIEAQK 420
Query: 421 LESALGIIMQDGSPKDLTAAFPLPASHLGTQQRPLISSYLNLTRIPTATILKSTELKLEA 480
L A+G I+++ P D P S L + SY+N T+ P AT+LKS E+ +
Sbjct: 421 L-GAVGSIVKNPEP-DRAFIRSFPVSFLSNDDYKSLVSYMNSTKNPKATVLKSEEISNQR 480
Query: 481 APLVASFSSRGPNPTTPDILKPDVIGPGVEILAAWSPIRSPSDAKGDNRKLLFNIISGTS 540
APLVASFSSRGP+ DILKPD+ PGVEILAA+SP SP++++ D R++ ++++SGTS
Sbjct: 481 APLVASFSSRGPSSIVSDILKPDITAPGVEILAAYSPDSSPTESEFDTRRVKYSVLSGTS 540
Query: 541 MACPHATAVAAYVKSFHPSWSPAALKSALITTAFPMR--GDLYPEAEFAYGSGHINPLGA 600
MACPH VAAYVK+FHP WSP+ ++SA++TTA+PM G + EFAYGSGH++P+ A
Sbjct: 541 MACPHVAGVAAYVKTFHPQWSPSMIQSAIMTTAWPMNASGSGFVSTEFAYGSGHVDPIDA 600
Query: 601 VNPGLIYNASEIDYIRFLCGEGYNTTLLRIITKDNSTCS-PTNSGLVYDLNYPSFALFTY 660
+NPGL+Y ++ D+I FLCG Y + LRII+ DNSTC+ + L +LNYP+ +
Sbjct: 601 INPGLVYELTKADHINFLCGLNYTSDHLRIISGDNSTCTKEISKTLPRNLNYPTMSAKVS 660
Query: 661 ISTPFSQVYKRKVTNVGSTNSTYKATI--FAPSELNITVNPSTLSFKALEEELKFEVTIE 720
+ PF+ ++R VTNVG STY A + F S+L+I V+P LS K++ E+ F VT+
Sbjct: 661 GTKPFNITFQRTVTNVGMQKSTYNAKVVKFPGSKLSIKVSPRVLSMKSMNEKQSFMVTVS 720
Query: 721 GKINGS--IASASLVWDDGVHKVRSPIIVF 740
G+ SA+L+W DG H VRSPIIV+
Sbjct: 721 SDSIGTKQPVSANLIWSDGTHNVRSPIIVY 738
BLAST of Cmc09g0259781 vs. TAIR 10
Match:
AT5G59120.1 (subtilase 4.13 )
HSP 1 Score: 654.1 bits (1686), Expect = 1.4e-187
Identity = 357/753 (47.41%), Postives = 491/753 (65.21%), Query Frame = 0
Query: 1 MSSLSRLLFLCFCFSLLFFSSLSEEDDQYRKTYIVYMGSHPKDQVSSTPLSSHHHMRLLQ 60
M++L+ L C +LF SS+S D ++ YIVYMGS + TP S HM +LQ
Sbjct: 1 MATLAASSSLLSCLLVLFLSSVSAVTDD-KQVYIVYMGS-LSSRADYTPTSD--HMNILQ 60
Query: 61 EAIGSTFAPHCLLHSYKRSFNGFVAKLTETEAKKVSEMEGVISVFPNEELQLHTTRSWDF 120
E G + L+ SYKRSFNGF A+LTE+E ++V++M GV+SVFPN++LQL TT SWDF
Sbjct: 61 EVTGESSIEGRLVRSYKRSFNGFAARLTESERERVAKMVGVVSVFPNKKLQLQTTTSWDF 120
Query: 121 MGFSE--QVERVPSVESDIIVGVFDTGIWPESPSFLDHGYGPPPPKWKGSCEVSLNFSCN 180
MG E + +R P+VESD I+GV D+GI PES SF D G+GPPP KWKG C NF+CN
Sbjct: 121 MGLKEGIKTKRNPTVESDTIIGVIDSGITPESQSFSDKGFGPPPQKWKGVCSGGKNFTCN 180
Query: 181 NKIIGAQSYRSDGRYPIDDIKGPRDSNGHGTHAASTVAGGLVRQASMLGLGTGTARGGVP 240
NK+IGA+ Y S+ G RD +GHGTH AST AG V AS G+G GT RGGVP
Sbjct: 181 NKLIGARDYTSE---------GTRDMDGHGTHTASTAAGNAVVDASFFGIGNGTVRGGVP 240
Query: 241 SARIAAYKVCWSDTCSGADVLAAFDDAIADGVDIISLSVGPKRPIPNYFNDPIAIGTFHA 300
++R+AAYKVC CS +L+AFDDAIADGVD+I++S+G K + NDPIAIG FHA
Sbjct: 241 ASRVAAYKVCTPTGCSSEALLSAFDDAIADGVDLITISIGDK-TASMFQNDPIAIGAFHA 300
Query: 301 MRNGILTSSSAGNEGPRPFTLTNFSPWALSVAASTTDRRFLTAVQLGDGRKFNGVTINTF 360
M G+LT +SAGN GP+P +++ +PW L+VAASTT+R F+T V LG+G+ G ++N +
Sbjct: 301 MAKGVLTVNSAGNSGPKPISVSGVAPWILTVAASTTNRGFVTKVVLGNGKTLVGKSVNAY 360
Query: 361 DLNGTQYPLVHAGNIPNVTGGFNGSISRFCLGNSVDRELVKGKIAVCDSIVLPSDVGSLE 420
++ G YPLV+ + + + + C + VD+ VKGKI VC P + +E
Sbjct: 361 EMKGKDYPLVYGKSA--ASSACDAESAGLCELSCVDKSRVKGKILVCGG---PGGLKIVE 420
Query: 421 S--ALGIIMQDGSPKDLTAAFPLPASHLGTQQRPLISSYLNLTRIPTATILKSTELKLEA 480
S A+G+I + P D+ PLPA+ L T+ + SYL T P A +LK+ +
Sbjct: 421 SVGAVGLIYRTPKP-DVAFIHPLPAAGLLTEDFESLVSYLESTDSPQAIVLKTEAIFNRT 480
Query: 481 APLVASFSSRGPNPTTPDILKPDVIGPGVEILAAWSPIRSPSDAKGDNRKLLFNIISGTS 540
+P++ASFSSRGPN DILKPD+ PGVEILAA+SP PS + D R + ++++SGTS
Sbjct: 481 SPVIASFSSRGPNTIAVDILKPDITAPGVEILAAYSPAGEPS--QDDTRHVKYSVLSGTS 540
Query: 541 MACPHATAVAAYVKSFHPSWSPAALKSALITTAFPMR--GDLYPEAEFAYGSGHINPLGA 600
M+CPH VAAYVK+F+P WSP+ ++SA++TTA+P+ G EFAYGSGH++P+ A
Sbjct: 541 MSCPHVAGVAAYVKTFNPKWSPSMIQSAIMTTAWPVNATGTGIASTEFAYGSGHVDPIAA 600
Query: 601 VNPGLIYNASEIDYIRFLCGEGYNTTLLRIITKDNSTCSPTNSGLVYDLNYPSF-ALFTY 660
NPGL+Y + D+I FLCG Y + +L++I+ + TCS L +LNYPS A +
Sbjct: 601 SNPGLVYELDKSDHIAFLCGMNYTSQVLKVISGETVTCSEAKKILPRNLNYPSMSAKLSG 660
Query: 661 ISTPFSQVYKRKVTNVGSTNSTYKATIFA--PSELNITVNPSTLSFKALEEELKFEVTIE 720
T F+ + R +TNVG+ NSTY + + A S+L++ + PS LSFK + E+ F VT+
Sbjct: 661 SGTTFTVTFNRTLTNVGTPNSTYTSKVVAGHGSKLDVKITPSVLSFKTVNEKQSFTVTVT 720
Query: 721 G-KINGSI-ASASLVWDDGVHKVRSPIIVFDSD 743
G ++ + +SA+L+W DG H VRSPI+V+ SD
Sbjct: 721 GSNLDSEVPSSANLIWSDGTHNVRSPIVVYTSD 731
BLAST of Cmc09g0259781 vs. TAIR 10
Match:
AT5G59090.1 (subtilase 4.12 )
HSP 1 Score: 644.8 bits (1662), Expect = 8.4e-185
Identity = 353/749 (47.13%), Postives = 482/749 (64.35%), Query Frame = 0
Query: 1 MSSLSRLLFLCFCFSLLFFSSLSEEDDQYRKTYIVYMGSHPKDQVSSTPLSSHHHMRLLQ 60
M++L+ L +L SS+S D+ + YIVYMGS + P S HM +LQ
Sbjct: 1 MANLAASTCLYSWLLVLLLSSVSAIIDEDTQVYIVYMGS-LSSRADYIPTSD--HMSILQ 60
Query: 61 EAIGSTFAPHCLLHSYKRSFNGFVAKLTETEAKKVSEMEGVISVFPNEELQLHTTRSWDF 120
+ G + L+ SYKRSFNGF A+LTE+E ++E+EGV+SVFPN+ LQLHTT SWDF
Sbjct: 61 QVTGESSIEGRLVRSYKRSFNGFAARLTESERTLIAEIEGVVSVFPNKILQLHTTTSWDF 120
Query: 121 MGFSE--QVERVPSVESDIIVGVFDTGIWPESPSFLDHGYGPPPPKWKGSCEVSLNFSCN 180
MG E +R ++ESD I+GV DTGIWPES SF D G+GPPP KWKG C NF+CN
Sbjct: 121 MGVKEGKNTKRNLAIESDTIIGVIDTGIWPESKSFSDKGFGPPPKKWKGVCSGGKNFTCN 180
Query: 181 NKIIGAQSYRSDGRYPIDDIKGPRDSNGHGTHAASTVAGGLVRQASMLGLGTGTARGGVP 240
NK+IGA+ Y S+ G RD++GHGTH AST AG V+ S G+G GT RGGVP
Sbjct: 181 NKLIGARDYTSE---------GTRDTSGHGTHTASTAAGNAVKDTSFFGIGNGTVRGGVP 240
Query: 241 SARIAAYKVCWSDTCSGADVLAAFDDAIADGVDIISLSVGPKRPIPNYFNDPIAIGTFHA 300
++RIAAYKVC CS +L++FDDAIADGVD+I++S+G + P + +DPIAIG FHA
Sbjct: 241 ASRIAAYKVCTDSGCSSEALLSSFDDAIADGVDLITISIGFQFP-SIFEDDPIAIGAFHA 300
Query: 301 MRNGILTSSSAGNEGPRPFTLTNFSPWALSVAASTTDRRFLTAVQLGDGRKFNGVTINTF 360
M GILT SSAGN GP+P T+++ +PW +VAASTT+R F+T V LG+G+ G ++N F
Sbjct: 301 MAKGILTVSSAGNSGPKPTTVSHVAPWIFTVAASTTNRGFITKVVLGNGKTLAGRSVNAF 360
Query: 361 DLNGTQYPLVHAGNIPNVTGGFNGSISRFCLGNSVDRELVKGKIAVCDSIVLPSDVGSLE 420
D+ G +YPLV+ + + + + C +++ VKGKI VC PS +
Sbjct: 361 DMKGKKYPLVYGKSA--ASSACDAKTAALCAPACLNKSRVKGKILVCGG---PSGYKIAK 420
Query: 421 SALGIIMQDGSPK-DLTAAFPLPASHLGTQQRPLISSYLNLTRIPTATILKSTELKLEAA 480
S I + D SP+ D+ LPAS L + + SY+ P A +LK+ + +
Sbjct: 421 SVGAIAIIDKSPRPDVAFTHHLPASGLKAKDFKSLVSYIESQDSPQAAVLKTETIFNRTS 480
Query: 481 PLVASFSSRGPNPTTPDILKPDVIGPGVEILAAWSPIRSPSDAKGDNRKLLFNIISGTSM 540
P++ASFSSRGPN DILKPD+ PGVEILAA+SP PS+ D R++ +++ SGTSM
Sbjct: 481 PVIASFSSRGPNTIAVDILKPDITAPGVEILAAFSPNGEPSE--DDTRRVKYSVFSGTSM 540
Query: 541 ACPHATAVAAYVKSFHPSWSPAALKSALITTAFPM--RGDLYPEAEFAYGSGHINPLGAV 600
ACPH VAAYVK+F+P WSP+ ++SA++TTA+P+ +G EFAYG+GH++P+ A+
Sbjct: 541 ACPHVAGVAAYVKTFYPRWSPSMIQSAIMTTAWPVKAKGRGIASTEFAYGAGHVDPMAAL 600
Query: 601 NPGLIYNASEIDYIRFLCGEGYNTTLLRIITKDNSTCSPTNSGLVYDLNYPSF-ALFTYI 660
NPGL+Y + D+I FLCG Y + L+II+ D CS N L +LNYPS A +
Sbjct: 601 NPGLVYELDKADHIAFLCGMNYTSKTLKIISGDTVKCSKKNKILPRNLNYPSMSAKLSGT 660
Query: 661 STPFSQVYKRKVTNVGSTNSTYKATIFA--PSELNITVNPSTLSFKALEEELKFEVTIEG 720
+ FS + R +TNVG+ NSTYK+ + A S+L+I V PS L FK + E+ F VT+ G
Sbjct: 661 DSTFSVTFNRTLTNVGTPNSTYKSKVVAGHGSKLSIKVTPSVLYFKTVNEKQSFSVTVTG 720
Query: 721 K-INGSI-ASASLVWDDGVHKVRSPIIVF 740
++ + +SA+L+W DG H VRSPI+V+
Sbjct: 721 SDVDSEVPSSANLIWSDGTHNVRSPIVVY 729
BLAST of Cmc09g0259781 vs. TAIR 10
Match:
AT5G58840.1 (Subtilase family protein )
HSP 1 Score: 643.7 bits (1659), Expect = 1.9e-184
Identity = 350/741 (47.23%), Postives = 464/741 (62.62%), Query Frame = 0
Query: 8 LFLCFCFSLLFFSSLSEEDDQYRKTYIVYMGSHPKDQVSSTPLSSHHHMRLLQEAIGSTF 67
L C S + S +D Q ++ Y+VYMGS P ++ TP+S HHM +LQE G +
Sbjct: 10 LISCVLVSFVISVSAVTDDSQDKQVYVVYMGSLPSSRLEYTPMS--HHMSILQEVTGESS 69
Query: 68 APHCLLHSYKRSFNGFVAKLTETEAKKVSEMEGVISVFPNEELQLHTTRSWDFMGFSE-- 127
L+ SYKRSFNGF A+LTE+E ++V+EMEGV+SVFP+ +L TT SWDF+G E
Sbjct: 70 VEGRLVRSYKRSFNGFAARLTESERERVAEMEGVVSVFPDINYKLQTTASWDFLGLKEGK 129
Query: 128 QVERVPSVESDIIVGVFDTGIWPESPSFLDHGYGPPPPKWKGSCEVSLNFSCNNKIIGAQ 187
+R ++ESD I+G D+GIWPES SF D G+GPPP KWKG C NF+CNNK+IGA+
Sbjct: 130 NTKRNLAIESDTIIGFIDSGIWPESESFSDKGFGPPPKKWKGVCSAGKNFTCNNKLIGAR 189
Query: 188 SYRSDGRYPIDDIKGPRDSNGHGTHAASTVAGGLVRQASMLGLGTGTARGGVPSARIAAY 247
Y ++ G RD GHGTH AST AG V+ S G+G GTARGGVP++RIAAY
Sbjct: 190 DYTNE---------GTRDIEGHGTHTASTAAGNAVKNTSFYGIGNGTARGGVPASRIAAY 249
Query: 248 KVCWSDTCSGADVLAAFDDAIADGVDIISLSVGPKRPIPNYFNDPIAIGTFHAMRNGILT 307
K C C+ VL+AFDDAIADGVD+IS+S+G + Y DPIAIG FHAM GILT
Sbjct: 250 KACSEMGCTTESVLSAFDDAIADGVDLISISLGANL-VRTYETDPIAIGAFHAMVKGILT 309
Query: 308 SSSAGNEGPRPFTLTNFSPWALSVAASTTDRRFLTAVQLGDGRKFNGVTINTFDLNGTQY 367
SAGN GP P ++ + +PW L+VAAS T+R F+T V LG+G+ F G ++N FDL G Y
Sbjct: 310 VQSAGNGGPNPGSVMSVAPWILTVAASNTNRGFVTKVVLGNGKTFVGKSLNAFDLKGKNY 369
Query: 368 PLVHAGNIPNVTGGFNGSISRFCLGNSVDRELVKGKIAVCDSIVLPSDVGSLESALGIIM 427
PL G S D L++GKI V + + S + +
Sbjct: 370 PL---------------------YGGSTDGPLLRGKILVSED--------KVSSEIVVAN 429
Query: 428 QDGSPKDLTAAFPLPASHLGTQQRPLISSYLNLTRIPTATILKSTELKLEAAPLVASFSS 487
+ + D LP+S L + SY+N T+ P T+LKS + +AAP VA FSS
Sbjct: 430 INENYHDYAYVSILPSSALSKDDFDSVISYVNSTKSPHGTVLKSEAIFNQAAPKVAGFSS 489
Query: 488 RGPNPTTPDILKPDVIGPGVEILAAWSPIRSPSDAKGDNRKLLFNIISGTSMACPHATAV 547
RGPN DILKPDV PGVEILAA+SP+ SP+ K DNR + ++++SGTSM+CPH V
Sbjct: 490 RGPNTIAVDILKPDVTAPGVEILAAFSPLNSPAQDKRDNRHVKYSVLSGTSMSCPHVAGV 549
Query: 548 AAYVKSFHPSWSPAALKSALITTAFPMR--GDLYPEAEFAYGSGHINPLGAVNPGLIYNA 607
AAY+K+FHP WSP+ ++SA++TTA+PM G EFAYG+GH++P+ A+NPGL+Y
Sbjct: 550 AAYIKTFHPEWSPSMIQSAIMTTAWPMNATGTAVASTEFAYGAGHVDPIAAINPGLVYEI 609
Query: 608 SEIDYIRFLCGEGYNTTLLRIITKDNSTCSPTNSGLVYDLNYPSF-ALFTYISTPFSQVY 667
+ D+I FLCG YN T L++I + TC T L +LNYPS A + F +
Sbjct: 610 GKSDHIAFLCGLNYNATSLKLIAGEAVTC--TGKTLPRNLNYPSMSAKLPKSESSFIVTF 669
Query: 668 KRKVTNVGSTNSTYKATIFA--PSELNITVNPSTLSFKALEEELKFEVTIEG-KINGSI- 727
R VTNVG+ NSTYK+ I S L + V+PS LS K+++E+ F VT+ G I+ +
Sbjct: 670 NRTVTNVGTPNSTYKSKIVLNHGSNLKVEVSPSVLSMKSVKEKQSFTVTVSGSNIDPKLP 707
Query: 728 ASASLVWDDGVHKVRSPIIVF 740
+SA+L+W DG H VRSPI+V+
Sbjct: 730 SSANLIWSDGTHNVRSPIVVY 707
The following BLAST results are available for this feature:
Match Name | E-value | Identity | Description | |
Q39547 | 1.1e-232 | 57.10 | Cucumisin OS=Cucumis melo OX=3656 PE=1 SV=1 | [more] |
Q9FIF8 | 1.3e-193 | 49.40 | Subtilisin-like protease SBT4.3 OS=Arabidopsis thaliana OX=3702 GN=SBT4.3 PE=3 S... | [more] |
Q9FGU3 | 2.7e-188 | 47.73 | Subtilisin-like protease SBT4.4 OS=Arabidopsis thaliana OX=3702 GN=SBT4.4 PE=2 S... | [more] |
Q9FIG2 | 2.0e-186 | 47.41 | Subtilisin-like protease SBT4.13 OS=Arabidopsis thaliana OX=3702 GN=SBT4.13 PE=2... | [more] |
Q8L7D2 | 1.2e-183 | 47.13 | Subtilisin-like protease SBT4.12 OS=Arabidopsis thaliana OX=3702 GN=SBT4.12 PE=2... | [more] |
Match Name | E-value | Identity | Description | |
A0A1S3AUC4 | 0.0e+00 | 99.73 | cucumisin-like isoform X1 OS=Cucumis melo OX=3656 GN=LOC103482910 PE=3 SV=1 | [more] |
A0A1S3AUU4 | 0.0e+00 | 99.72 | cucumisin-like isoform X2 OS=Cucumis melo OX=3656 GN=LOC103482910 PE=3 SV=1 | [more] |
A0A1S3AUT1 | 0.0e+00 | 99.56 | cucumisin-like isoform X3 OS=Cucumis melo OX=3656 GN=LOC103482910 PE=3 SV=1 | [more] |
A0A0A0KJN5 | 0.0e+00 | 91.29 | Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G141040 PE=3 SV=1 | [more] |
A0A6J1E2G4 | 0.0e+00 | 77.69 | cucumisin-like OS=Cucurbita moschata OX=3662 GN=LOC111430231 PE=3 SV=1 | [more] |