Cmc09g0259141 (gene) Melon (Charmono) v1.1

Overview
NameCmc09g0259141
Typegene
OrganismCucumis melo L. var. cantalupensis cv. Charmono (Melon (Charmono) v1.1)
DescriptionAB hydrolase-1 domain-containing protein
LocationCMiso1.1chr09: 22966693 .. 22970866 (+)
RNA-Seq ExpressionCmc09g0259141
SyntenyCmc09g0259141
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GATTTCTTAACACCCTACCTAATACAACTAAAATCATATGTAGAGCTCCTCATGTTTGAACATTTTGTTTGCATTTTTAACGTGGGATTTAACAAACAGACAGTAGAGTACTTGTGTGGTAGAATGATCCATTGGAAAACCGTGTCGGCCAATTATCAACCTTTTACTTTCTTACACGAAATACATATGGATGTGAGATAATTTGATAGATTTTGCGTGGAAGTTCAATAGAACAATGTAATTTAATCGTAAGAAAACAAAAATGTAATCTTTTGAAATTTTTAGTAACTTTAAAATATATTTTTCTAGAAGAAAAAGAAAAAAAATCCAACCAATATCAATCAACGGTCGATGACATCTCTGATCGGAACTTGGGCACCACACCGGCGGGGCCCTCCTCCGATCAGACTGCTATGCAAATTCAAGATGATGACATGTTCATGACTCTTAATAATAATCACAGCCGTTCCTCACCCATCTCCAATATTCCGATTCCTTCTCATTCCCCACCAATGGCGAGAGCCTTCTTAGGCTCCACGTCTTCCTCTTCCCCACTAATCGCTTTACGCATCTCTTCCTTCACCCCATCATTTTTCTTAACCAATCAACGCCCCCGCTCCCTTCGTCCCACCACCGGGTCCGTCGTCGCTATGGCGGAGGTTCCGTCGGAGAAGCGCATTAAGCCCCACCCATCTCTCGAGGTCATCGGTGGAGGCTGCGATCAGTTTCTCCCAGCTTTTAAGGACTTGGATCTCCCCTATAAGCCTTTCCCTGTAATTGGGTCGAATCGCCATTTGGAGACCATTTTTGCTTCCTTTTTTAGGACTTGTCCCAGTGTTAACCTCCACCGGGAGTGTCTCCGTGCGGCAGATAATGGGACCGTTGCTCTCGATTGGGTCACCGGTGACGATCTCCGTTTGCCTCTTGATTCTCCTGTTCTTATTCTTCTGGTCTGTGCTTAATGATTCTTGGTTTTTTGAAGAATTTATGGATTAGAAAGAAATGGCAAAGATATTCCTATACCAAAATTATATGAATCGATTTGGGCATCCTTGAAAAGAAACGCTTTTACATACCCAATTCGTTAATCCATTTAATCCTAATTGTAATTACTCTATGGTCTATAGCTCTTGTGTTGCTCTTTTTTCCTTTTTTTTTTCCTTTTTTGTGTGTGTTTTTTGTTTCGCGTCTCCCATTGAGGGGCTTAATTTTTCCTCTCTTTAGTAAAACCTCCTAAGGAAGTGCAAGTTCTTTGCTTGGAAACCTTTACATGGTAGAATGTGGCATTCTTCTTAAATTCTATGGCTAAAGTGTGCCCTGCTTATCACCTACCTAGTATTTAATATCTTGTGCATTTCTTGACACCCAATTATTTTAGAGACAATTATCTTGTGAGGTTAGTCGAGGTGCACATCTGGTTTGGACACACACAGGTTAGAAAAAAGAAAGTAGTGTGTCCTTTTTGGTGCTTCATAGGTACTACTGCTCTATAGTGGGGAAGACTCTACAAACTCTCCCTTTTCAGGTAAAGGAAGCGTAGGAGGTTGTGAGATTTAATCGTCTTTATGGGTGTTTGGATAGATCATTGTTTAACTGTCAGCTTGTAGTAGTTAGTTTGGAGGTTAGGCGTGGTTTATGGAGAAATCAGTGCTGATTCTGGGAGAGAAATATTTCCCTTGAAAGGCTATTGATTTTCTAATTTTACCTTTGATATTGCGATATAGTTTTACTGTCGATTTATTGTAACTAGGATCGTTATAGTTCCTTGATTCATATCAAAAGATTTCATATTTTTTTTCTTGAACTTCTGCAGCCTGGTTTAACAGGAGGGAGTCAGGATTCATATGTAAGACACATGTTGGTTAGAGCTAGAGATCGTGGGTGGAGGGTTGTGGTTTTCAACAGCCGTGGATGTGGGAACAGCCCTGTTACCACTCCTCAGGTATCCATTTTTTGTTCTTTATGTTGTATTCAGCATCATATTGCTTTGACATCAGATGATTTTTCTTCTTTTCAATATTCGGAATTGACATTCTTGTTTATGATTCACTTTACAACACTGATATATGCAGTTCTATTCGGCTTCATTTTTAGGAGATATGCGTGAAGTAGTTGCGCATGTTACTAAAAGATATCCCAAGGCTAATGTGTATGCTGTTGGCTGGTCACTTGGTGGTAACATTCTTGTTAATTATTTGGGACAGGTAAAAGTAAAACAATACATATCAGTCCCCATCACTCTTTACTCTACTTATTGGTTGCCATTTTGTTTAAACCTCCCGAGTCTCATGGAATGGGGGAAATTGGGGTATTTAATTCAAATTTGTGAGGAAAAGTCATCTTTATCTCTTACATATCACTCCGACATCTTCATTGCAGGATTCCCTGACCTGTCCATTGTCTGGTGCTGTGTCTTTATGTAATCCATTCGATTTAATAGTTGCAGATGAGGACTTTCGCAAGGGCTTCAACAACGTCTATGATAAATCTCTGGCTAGAGCGTTACGTAAAATTTTCAACAAGTAATTCTCTTAGCTGAATACGTTTTGTTTTGTTATTGAGTCATAATCTGAAACTGAAACATTGGTCGTTCCTTAGATTGTAAAATGTATCATCTCACTAGATTAATTCCCCTGAGATTGCAGGCATGCTGCTCTCTTCACTGATATGCCTAGTGAATTCAATATACGCTTGGCTGCCAAAGCTAGAACTGTTAGAGACTATGATGATGCCTTAACACGCGGTATACTTTCTCTTAATTTTTGTTATTTTCATTCGAAAAACATTACCATACATTCCATTTCTTCTCCTCAACCTTTGTTTTTACTTTATCTTATTGTGTTTTTTTTTCTTTGAACAACTTTCAGTTTCATTTGGTTTCAACACTGTGGATGAATACTACTCAAAGTCTAGCAGTTCAAAGTTCATAAAAGATGTCAAAATTCCTTTGCTTTGTATCCAGGTACCATTTTTGTTTTTTCAAATGTTGTCTTGTCTAATTGTTCTTTTAGAACTCAAAATTGCTGTCAAATCTCTTTTAAGTTATTACCTGTGAGGGAAATAATATATTGTATTTACTATTTTGAAACAGTTTCCAAACAACTCTTAAACTATCTTTTCTCCATTAACTTCTTGTTAATTGATTTGGGAAGTTACAGAATGTCTAGGGAAATTGTAGCTAAGAACACTGTTCTCTCGCAGATATTTTTAGGCATTCTGTTCTTGTAATGTTAAGGGGCATTTAATTTGGTTTTCAGGCAGCAAATGATCCAATTGCTCCAAACAGAGGAATCCCCCGAGAAGATATTAAGGTTTCACACATGTTTATCTTCAAGTTTTATGAGTTATCTGTTAAAAATCTAGTGAAAAGTTTAGTGCCATTTCATAACTATTTGATTTTTCATCTTTGGAAATTAGGCTTATAAACACTACTTCGATGTATTGGTTTATTTGTCTTGTTATCTACTTTTTGAAAAATGTTTCTAAGTTCATGATCAAGTGGAACCATACTTTCTTTTTTTTTTTTTTAGTTTTGCTTTTGTTTTTAGAATTTGACTAAAGATTCAAATGCGATTTCACGTTCGGACTTCAAGTGTTTAATGCACCATTCTTGTGATTAGTGAACATATTTTTCAAACGATGACTGTTGTTGCAGGAAAATCCAAACTGTATGTTAATAGTAACACCGAGAGGTGGGCATCTAGGGTGGGTTGCAGGGCCCGGCGCTCCCCTTGGTTTTCCATGGACCGATCCTTTCGTGATCGATTTCCTCATGTATTTAGAAAACCAAAAATCCAAAGCCTCAGCTTCTTCTACAGATTTAACAACGGCAGAGGTAATGACATAGCCATTGATGGTGATGATATCTACGAGCACGAGTAGAAACATCGAAGATAGTCGTTCGCTATCCCTAGCTAGTAGTTGATTATTTGTGAGATGATTAAAACATAATGTCATGTATATAGTTTATTTAAAAGTGTCAAATGCAGGTCTCAATCTTAATCAAAGTTATTTGTTTCTGTATTTCTTTATGGACCCTTTTAGGTACTTTATCTTGGGAATATTTTTTTTATAATATTTATAGAGATCGTTATTTTGATTGTTCTTTGTAATGTATGTGATTTTCTTCCCATGAATTTTTATGATATTGGTGGGCTGATTGAAAATTATGAAC

mRNA sequence

GATTTCTTAACACCCTACCTAATACAACTAAAATCATATGTAGAGCTCCTCATGTTTGAACATTTTGTTTGCATTTTTAACGTGGGATTTAACAAACAGACAGTAGAGTACTTGTGTGGTAGAATGATCCATTGGAAAACCGTGTCGGCCAATTATCAACCTTTTACTTTCTTACACGAAATACATATGGATGTGAGATAATTTGATAGATTTTGCGTGGAAGTTCAATAGAACAATGTAATTTAATCGTAAGAAAACAAAAATGTAATCTTTTGAAATTTTTAGTAACTTTAAAATATATTTTTCTAGAAGAAAAAGAAAAAAAATCCAACCAATATCAATCAACGGTCGATGACATCTCTGATCGGAACTTGGGCACCACACCGGCGGGGCCCTCCTCCGATCAGACTGCTATGCAAATTCAAGATGATGACATGTTCATGACTCTTAATAATAATCACAGCCGTTCCTCACCCATCTCCAATATTCCGATTCCTTCTCATTCCCCACCAATGGCGAGAGCCTTCTTAGGCTCCACGTCTTCCTCTTCCCCACTAATCGCTTTACGCATCTCTTCCTTCACCCCATCATTTTTCTTAACCAATCAACGCCCCCGCTCCCTTCGTCCCACCACCGGGTCCGTCGTCGCTATGGCGGAGGTTCCGTCGGAGAAGCGCATTAAGCCCCACCCATCTCTCGAGGTCATCGGTGGAGGCTGCGATCAGTTTCTCCCAGCTTTTAAGGACTTGGATCTCCCCTATAAGCCTTTCCCTGTAATTGGGTCGAATCGCCATTTGGAGACCATTTTTGCTTCCTTTTTTAGGACTTGTCCCAGTGTTAACCTCCACCGGGAGTGTCTCCGTGCGGCAGATAATGGGACCGTTGCTCTCGATTGGGTCACCGGTGACGATCTCCGTTTGCCTCTTGATTCTCCTGTTCTTATTCTTCTGCCTGGTTTAACAGGAGGGAGTCAGGATTCATATGTAAGACACATGTTGGTTAGAGCTAGAGATCGTGGGTGGAGGGTTGTGGTTTTCAACAGCCGTGGATGTGGGAACAGCCCTGTTACCACTCCTCAGTTCTATTCGGCTTCATTTTTAGGAGATATGCGTGAAGTAGTTGCGCATGTTACTAAAAGATATCCCAAGGCTAATGTGTATGCTGTTGGCTGGTCACTTGGTGGTAACATTCTTGTTAATTATTTGGGACAGGATTCCCTGACCTGTCCATTGTCTGGTGCTGTGTCTTTATGTAATCCATTCGATTTAATAGTTGCAGATGAGGACTTTCGCAAGGGCTTCAACAACGTCTATGATAAATCTCTGGCTAGAGCGTTACGTAAAATTTTCAACAAGCATGCTGCTCTCTTCACTGATATGCCTAGTGAATTCAATATACGCTTGGCTGCCAAAGCTAGAACTGTTAGAGACTATGATGATGCCTTAACACGCGTTTCATTTGGTTTCAACACTGTGGATGAATACTACTCAAAGTCTAGCAGTTCAAAGTTCATAAAAGATGTCAAAATTCCTTTGCTTTGTATCCAGGCAGCAAATGATCCAATTGCTCCAAACAGAGGAATCCCCCGAGAAGATATTAAGGAAAATCCAAACTGTATGTTAATAGTAACACCGAGAGGTGGGCATCTAGGGTGGGTTGCAGGGCCCGGCGCTCCCCTTGGTTTTCCATGGACCGATCCTTTCGTGATCGATTTCCTCATGTATTTAGAAAACCAAAAATCCAAAGCCTCAGCTTCTTCTACAGATTTAACAACGGCAGAGGTAATGACATAGCCATTGATGGTGATGATATCTACGAGCACGAGTAGAAACATCGAAGATAGTCGTTCGCTATCCCTAGCTAGTAGTTGATTATTTGTGAGATGATTAAAACATAATGTCATGTATATAGTTTATTTAAAAGTGTCAAATGCAGGTCTCAATCTTAATCAAAGTTATTTGTTTCTGTATTTCTTTATGGACCCTTTTAGGTACTTTATCTTGGGAATATTTTTTTTATAATATTTATAGAGATCGTTATTTTGATTGTTCTTTGTAATGTATGTGATTTTCTTCCCATGAATTTTTATGATATTGGTGGGCTGATTGAAAATTATGAAC

Coding sequence (CDS)

ATGCAAATTCAAGATGATGACATGTTCATGACTCTTAATAATAATCACAGCCGTTCCTCACCCATCTCCAATATTCCGATTCCTTCTCATTCCCCACCAATGGCGAGAGCCTTCTTAGGCTCCACGTCTTCCTCTTCCCCACTAATCGCTTTACGCATCTCTTCCTTCACCCCATCATTTTTCTTAACCAATCAACGCCCCCGCTCCCTTCGTCCCACCACCGGGTCCGTCGTCGCTATGGCGGAGGTTCCGTCGGAGAAGCGCATTAAGCCCCACCCATCTCTCGAGGTCATCGGTGGAGGCTGCGATCAGTTTCTCCCAGCTTTTAAGGACTTGGATCTCCCCTATAAGCCTTTCCCTGTAATTGGGTCGAATCGCCATTTGGAGACCATTTTTGCTTCCTTTTTTAGGACTTGTCCCAGTGTTAACCTCCACCGGGAGTGTCTCCGTGCGGCAGATAATGGGACCGTTGCTCTCGATTGGGTCACCGGTGACGATCTCCGTTTGCCTCTTGATTCTCCTGTTCTTATTCTTCTGCCTGGTTTAACAGGAGGGAGTCAGGATTCATATGTAAGACACATGTTGGTTAGAGCTAGAGATCGTGGGTGGAGGGTTGTGGTTTTCAACAGCCGTGGATGTGGGAACAGCCCTGTTACCACTCCTCAGTTCTATTCGGCTTCATTTTTAGGAGATATGCGTGAAGTAGTTGCGCATGTTACTAAAAGATATCCCAAGGCTAATGTGTATGCTGTTGGCTGGTCACTTGGTGGTAACATTCTTGTTAATTATTTGGGACAGGATTCCCTGACCTGTCCATTGTCTGGTGCTGTGTCTTTATGTAATCCATTCGATTTAATAGTTGCAGATGAGGACTTTCGCAAGGGCTTCAACAACGTCTATGATAAATCTCTGGCTAGAGCGTTACGTAAAATTTTCAACAAGCATGCTGCTCTCTTCACTGATATGCCTAGTGAATTCAATATACGCTTGGCTGCCAAAGCTAGAACTGTTAGAGACTATGATGATGCCTTAACACGCGTTTCATTTGGTTTCAACACTGTGGATGAATACTACTCAAAGTCTAGCAGTTCAAAGTTCATAAAAGATGTCAAAATTCCTTTGCTTTGTATCCAGGCAGCAAATGATCCAATTGCTCCAAACAGAGGAATCCCCCGAGAAGATATTAAGGAAAATCCAAACTGTATGTTAATAGTAACACCGAGAGGTGGGCATCTAGGGTGGGTTGCAGGGCCCGGCGCTCCCCTTGGTTTTCCATGGACCGATCCTTTCGTGATCGATTTCCTCATGTATTTAGAAAACCAAAAATCCAAAGCCTCAGCTTCTTCTACAGATTTAACAACGGCAGAGGTAATGACATAG

Protein sequence

MQIQDDDMFMTLNNNHSRSSPISNIPIPSHSPPMARAFLGSTSSSSPLIALRISSFTPSFFLTNQRPRSLRPTTGSVVAMAEVPSEKRIKPHPSLEVIGGGCDQFLPAFKDLDLPYKPFPVIGSNRHLETIFASFFRTCPSVNLHRECLRAADNGTVALDWVTGDDLRLPLDSPVLILLPGLTGGSQDSYVRHMLVRARDRGWRVVVFNSRGCGNSPVTTPQFYSASFLGDMREVVAHVTKRYPKANVYAVGWSLGGNILVNYLGQDSLTCPLSGAVSLCNPFDLIVADEDFRKGFNNVYDKSLARALRKIFNKHAALFTDMPSEFNIRLAAKARTVRDYDDALTRVSFGFNTVDEYYSKSSSSKFIKDVKIPLLCIQAANDPIAPNRGIPREDIKENPNCMLIVTPRGGHLGWVAGPGAPLGFPWTDPFVIDFLMYLENQKSKASASSTDLTTAEVMT
Homology
BLAST of Cmc09g0259141 vs. NCBI nr
Match: KAA0042649.1 (embryogenesis-associated protein EMB8 [Cucumis melo var. makuwa] >TYK06050.1 embryogenesis-associated protein EMB8 [Cucumis melo var. makuwa])

HSP 1 Score: 939.1 bits (2426), Expect = 1.4e-269
Identity = 459/459 (100.00%), Postives = 459/459 (100.00%), Query Frame = 0

Query: 1   MQIQDDDMFMTLNNNHSRSSPISNIPIPSHSPPMARAFLGSTSSSSPLIALRISSFTPSF 60
           MQIQDDDMFMTLNNNHSRSSPISNIPIPSHSPPMARAFLGSTSSSSPLIALRISSFTPSF
Sbjct: 1   MQIQDDDMFMTLNNNHSRSSPISNIPIPSHSPPMARAFLGSTSSSSPLIALRISSFTPSF 60

Query: 61  FLTNQRPRSLRPTTGSVVAMAEVPSEKRIKPHPSLEVIGGGCDQFLPAFKDLDLPYKPFP 120
           FLTNQRPRSLRPTTGSVVAMAEVPSEKRIKPHPSLEVIGGGCDQFLPAFKDLDLPYKPFP
Sbjct: 61  FLTNQRPRSLRPTTGSVVAMAEVPSEKRIKPHPSLEVIGGGCDQFLPAFKDLDLPYKPFP 120

Query: 121 VIGSNRHLETIFASFFRTCPSVNLHRECLRAADNGTVALDWVTGDDLRLPLDSPVLILLP 180
           VIGSNRHLETIFASFFRTCPSVNLHRECLRAADNGTVALDWVTGDDLRLPLDSPVLILLP
Sbjct: 121 VIGSNRHLETIFASFFRTCPSVNLHRECLRAADNGTVALDWVTGDDLRLPLDSPVLILLP 180

Query: 181 GLTGGSQDSYVRHMLVRARDRGWRVVVFNSRGCGNSPVTTPQFYSASFLGDMREVVAHVT 240
           GLTGGSQDSYVRHMLVRARDRGWRVVVFNSRGCGNSPVTTPQFYSASFLGDMREVVAHVT
Sbjct: 181 GLTGGSQDSYVRHMLVRARDRGWRVVVFNSRGCGNSPVTTPQFYSASFLGDMREVVAHVT 240

Query: 241 KRYPKANVYAVGWSLGGNILVNYLGQDSLTCPLSGAVSLCNPFDLIVADEDFRKGFNNVY 300
           KRYPKANVYAVGWSLGGNILVNYLGQDSLTCPLSGAVSLCNPFDLIVADEDFRKGFNNVY
Sbjct: 241 KRYPKANVYAVGWSLGGNILVNYLGQDSLTCPLSGAVSLCNPFDLIVADEDFRKGFNNVY 300

Query: 301 DKSLARALRKIFNKHAALFTDMPSEFNIRLAAKARTVRDYDDALTRVSFGFNTVDEYYSK 360
           DKSLARALRKIFNKHAALFTDMPSEFNIRLAAKARTVRDYDDALTRVSFGFNTVDEYYSK
Sbjct: 301 DKSLARALRKIFNKHAALFTDMPSEFNIRLAAKARTVRDYDDALTRVSFGFNTVDEYYSK 360

Query: 361 SSSSKFIKDVKIPLLCIQAANDPIAPNRGIPREDIKENPNCMLIVTPRGGHLGWVAGPGA 420
           SSSSKFIKDVKIPLLCIQAANDPIAPNRGIPREDIKENPNCMLIVTPRGGHLGWVAGPGA
Sbjct: 361 SSSSKFIKDVKIPLLCIQAANDPIAPNRGIPREDIKENPNCMLIVTPRGGHLGWVAGPGA 420

Query: 421 PLGFPWTDPFVIDFLMYLENQKSKASASSTDLTTAEVMT 460
           PLGFPWTDPFVIDFLMYLENQKSKASASSTDLTTAEVMT
Sbjct: 421 PLGFPWTDPFVIDFLMYLENQKSKASASSTDLTTAEVMT 459

BLAST of Cmc09g0259141 vs. NCBI nr
Match: XP_008437445.1 (PREDICTED: LOW QUALITY PROTEIN: embryogenesis-associated protein EMB8 [Cucumis melo])

HSP 1 Score: 925.6 bits (2391), Expect = 1.6e-265
Identity = 452/452 (100.00%), Postives = 452/452 (100.00%), Query Frame = 0

Query: 8   MFMTLNNNHSRSSPISNIPIPSHSPPMARAFLGSTSSSSPLIALRISSFTPSFFLTNQRP 67
           MFMTLNNNHSRSSPISNIPIPSHSPPMARAFLGSTSSSSPLIALRISSFTPSFFLTNQRP
Sbjct: 1   MFMTLNNNHSRSSPISNIPIPSHSPPMARAFLGSTSSSSPLIALRISSFTPSFFLTNQRP 60

Query: 68  RSLRPTTGSVVAMAEVPSEKRIKPHPSLEVIGGGCDQFLPAFKDLDLPYKPFPVIGSNRH 127
           RSLRPTTGSVVAMAEVPSEKRIKPHPSLEVIGGGCDQFLPAFKDLDLPYKPFPVIGSNRH
Sbjct: 61  RSLRPTTGSVVAMAEVPSEKRIKPHPSLEVIGGGCDQFLPAFKDLDLPYKPFPVIGSNRH 120

Query: 128 LETIFASFFRTCPSVNLHRECLRAADNGTVALDWVTGDDLRLPLDSPVLILLPGLTGGSQ 187
           LETIFASFFRTCPSVNLHRECLRAADNGTVALDWVTGDDLRLPLDSPVLILLPGLTGGSQ
Sbjct: 121 LETIFASFFRTCPSVNLHRECLRAADNGTVALDWVTGDDLRLPLDSPVLILLPGLTGGSQ 180

Query: 188 DSYVRHMLVRARDRGWRVVVFNSRGCGNSPVTTPQFYSASFLGDMREVVAHVTKRYPKAN 247
           DSYVRHMLVRARDRGWRVVVFNSRGCGNSPVTTPQFYSASFLGDMREVVAHVTKRYPKAN
Sbjct: 181 DSYVRHMLVRARDRGWRVVVFNSRGCGNSPVTTPQFYSASFLGDMREVVAHVTKRYPKAN 240

Query: 248 VYAVGWSLGGNILVNYLGQDSLTCPLSGAVSLCNPFDLIVADEDFRKGFNNVYDKSLARA 307
           VYAVGWSLGGNILVNYLGQDSLTCPLSGAVSLCNPFDLIVADEDFRKGFNNVYDKSLARA
Sbjct: 241 VYAVGWSLGGNILVNYLGQDSLTCPLSGAVSLCNPFDLIVADEDFRKGFNNVYDKSLARA 300

Query: 308 LRKIFNKHAALFTDMPSEFNIRLAAKARTVRDYDDALTRVSFGFNTVDEYYSKSSSSKFI 367
           LRKIFNKHAALFTDMPSEFNIRLAAKARTVRDYDDALTRVSFGFNTVDEYYSKSSSSKFI
Sbjct: 301 LRKIFNKHAALFTDMPSEFNIRLAAKARTVRDYDDALTRVSFGFNTVDEYYSKSSSSKFI 360

Query: 368 KDVKIPLLCIQAANDPIAPNRGIPREDIKENPNCMLIVTPRGGHLGWVAGPGAPLGFPWT 427
           KDVKIPLLCIQAANDPIAPNRGIPREDIKENPNCMLIVTPRGGHLGWVAGPGAPLGFPWT
Sbjct: 361 KDVKIPLLCIQAANDPIAPNRGIPREDIKENPNCMLIVTPRGGHLGWVAGPGAPLGFPWT 420

Query: 428 DPFVIDFLMYLENQKSKASASSTDLTTAEVMT 460
           DPFVIDFLMYLENQKSKASASSTDLTTAEVMT
Sbjct: 421 DPFVIDFLMYLENQKSKASASSTDLTTAEVMT 452

BLAST of Cmc09g0259141 vs. NCBI nr
Match: XP_004143840.3 (embryogenesis-associated protein EMB8 [Cucumis sativus] >KGN49619.2 hypothetical protein Csa_000306 [Cucumis sativus])

HSP 1 Score: 889.8 bits (2298), Expect = 1.0e-254
Identity = 434/459 (94.55%), Postives = 444/459 (96.73%), Query Frame = 0

Query: 1   MQIQDDDMFMTLNNNHSRSSPISNIPIPSHSPPMARAFLGSTSSSSPLIALRISSFTPSF 60
           +QIQDD +   LNNNHSRSSPISNIPIPSHSPPMARAFLGST SSSPLIALR+SSFTPSF
Sbjct: 22  IQIQDDGIIRILNNNHSRSSPISNIPIPSHSPPMARAFLGSTPSSSPLIALRLSSFTPSF 81

Query: 61  FLTNQRPRSLRPTTGSVVAMAEVPSEKRIKPHPSLEVIGGGCDQFLPAFKDLDLPYKPFP 120
           FLTNQRPRSLR TT +VV MAE+PSEKRIKPHPSLEVIGGGCDQFLPAFKDLDLPYKPFP
Sbjct: 82  FLTNQRPRSLRLTTDTVVPMAELPSEKRIKPHPSLEVIGGGCDQFLPAFKDLDLPYKPFP 141

Query: 121 VIGSNRHLETIFASFFRTCPSVNLHRECLRAADNGTVALDWVTGDDLRLPLDSPVLILLP 180
           VIGSNRHLETIFASFFRTCPSVNLHRECLRAADNGTVALDWV GDDLRLPLDSPVLILLP
Sbjct: 142 VIGSNRHLETIFASFFRTCPSVNLHRECLRAADNGTVALDWVAGDDLRLPLDSPVLILLP 201

Query: 181 GLTGGSQDSYVRHMLVRARDRGWRVVVFNSRGCGNSPVTTPQFYSASFLGDMREVVAHVT 240
           GLTGGSQDSYVRHMLVRARDRGWRVVVFNSRGCGNSPVTTPQFYSASFLGDMREVVAHVT
Sbjct: 202 GLTGGSQDSYVRHMLVRARDRGWRVVVFNSRGCGNSPVTTPQFYSASFLGDMREVVAHVT 261

Query: 241 KRYPKANVYAVGWSLGGNILVNYLGQDSLTCPLSGAVSLCNPFDLIVADEDFRKGFNNVY 300
           +RYPKAN+YAVGWSLGGNILVNYLGQDSLTCPLSGAVSLCNPFDLIVADEDFRKGFNNVY
Sbjct: 262 ERYPKANLYAVGWSLGGNILVNYLGQDSLTCPLSGAVSLCNPFDLIVADEDFRKGFNNVY 321

Query: 301 DKSLARALRKIFNKHAALFTDMPSEFNIRLAAKARTVRDYDDALTRVSFGFNTVDEYYSK 360
           DKSLA ALRKIFNKHAALFTDM  EFNIRLAAKA TVRDYDDALTRVSFGFNTVDEYYSK
Sbjct: 322 DKSLAGALRKIFNKHAALFTDMTGEFNIRLAAKATTVRDYDDALTRVSFGFNTVDEYYSK 381

Query: 361 SSSSKFIKDVKIPLLCIQAANDPIAPNRGIPREDIKENPNCMLIVTPRGGHLGWVAGPGA 420
           SSSSKFIKDVKIPLLCIQAANDPIAPNRGIPREDI+ENPNCMLIVTP+GGHLGWVAGPGA
Sbjct: 382 SSSSKFIKDVKIPLLCIQAANDPIAPNRGIPREDIEENPNCMLIVTPKGGHLGWVAGPGA 441

Query: 421 PLGFPWTDPFVIDFLMYLENQKSKASASSTDLTTAEVMT 460
           PLGFPWTDPFVIDFLMYLEN+K KASASSTDLT AEVMT
Sbjct: 442 PLGFPWTDPFVIDFLMYLENKKFKASASSTDLTMAEVMT 480

BLAST of Cmc09g0259141 vs. NCBI nr
Match: XP_038875981.1 (embryogenesis-associated protein EMB8 [Benincasa hispida])

HSP 1 Score: 797.3 bits (2058), Expect = 6.7e-227
Identity = 387/426 (90.85%), Postives = 406/426 (95.31%), Query Frame = 0

Query: 34  MARAFLGSTSSSSPLIALRISSFTPSFFLTNQRPRSLRPTTGSVVAMAEVPSEKRIKPHP 93
           MARAFLGST SSSPLIALR SSFTPS F+TNQR RSLRPT  +V+AMAE+PSEKRI+PHP
Sbjct: 1   MARAFLGSTHSSSPLIALRFSSFTPS-FITNQRRRSLRPTPATVLAMAELPSEKRIQPHP 60

Query: 94  SLEVIGGGCDQFLPAFKDLDLPYKPFPVIGSNRHLETIFASFFRTCPSVNLHRECLRAAD 153
           SLEV+GGGCD+FLPAFKDLDLPYKPFPVIGSNRHLETIFASFFRTCP+V L RECLRAAD
Sbjct: 61  SLEVLGGGCDRFLPAFKDLDLPYKPFPVIGSNRHLETIFASFFRTCPNVKLRRECLRAAD 120

Query: 154 NGTVALDWVTGDDLRLPLDSPVLILLPGLTGGSQDSYVRHMLVRARDRGWRVVVFNSRGC 213
           NGTVALDWV GDDLRLPLDSPVLILLPGLTGGSQDSYVRHMLVRARDRGWRVVVFNSRGC
Sbjct: 121 NGTVALDWVAGDDLRLPLDSPVLILLPGLTGGSQDSYVRHMLVRARDRGWRVVVFNSRGC 180

Query: 214 GNSPVTTPQFYSASFLGDMREVVAHVTKRYPKANVYAVGWSLGGNILVNYLGQDSLTCPL 273
           GNSPVTTPQFYSASFLGDMREVVA VT+RYPKANVYAVGWSLGGNILVNYLGQDSL+CPL
Sbjct: 181 GNSPVTTPQFYSASFLGDMREVVALVTQRYPKANVYAVGWSLGGNILVNYLGQDSLSCPL 240

Query: 274 SGAVSLCNPFDLIVADEDFRKGFNNVYDKSLARALRKIFNKHAALFTDMPSEFNIRLAAK 333
           SGAVSLCNPFDLIVADEDFRKGFNNVYDK+LARAL KIFNKHA LF DMP EFNIRLAA 
Sbjct: 241 SGAVSLCNPFDLIVADEDFRKGFNNVYDKALARALHKIFNKHADLFNDMPGEFNIRLAAN 300

Query: 334 ARTVRDYDDALTRVSFGFNTVDEYYSKSSSSKFIKDVKIPLLCIQAANDPIAPNRGIPRE 393
           A+TVRDYD+ALTRVSFGFN+VDEYYSKSSSSKFIKDVKIPLLCIQAANDPIAP+RGIPRE
Sbjct: 301 AKTVRDYDNALTRVSFGFNSVDEYYSKSSSSKFIKDVKIPLLCIQAANDPIAPDRGIPRE 360

Query: 394 DIKENPNCMLIVTPRGGHLGWVAGPGAPLGFPWTDPFVIDFLMYLENQKSKASASSTDLT 453
           DIKENPNC+LIVTP+GGHLGWVAGPGAPLGFPWTDPFVIDFL+YLEN  +KASASS  LT
Sbjct: 361 DIKENPNCLLIVTPKGGHLGWVAGPGAPLGFPWTDPFVIDFLVYLENHTAKASASSIGLT 420

Query: 454 TAEVMT 460
            AEVMT
Sbjct: 421 PAEVMT 425

BLAST of Cmc09g0259141 vs. NCBI nr
Match: XP_022949852.1 (embryogenesis-associated protein EMB8-like [Cucurbita moschata])

HSP 1 Score: 775.8 bits (2002), Expect = 2.1e-220
Identity = 374/433 (86.37%), Postives = 400/433 (92.38%), Query Frame = 0

Query: 26  PIPSHSPPMARAFLGSTSSSSPLIALRISSFTPSFFLTNQRPRSLRPTTGSVVAMAEVPS 85
           PIP H PPMARAFLGST SSS L   R+SSFTPS  + NQR RSLR TT +VVAM+E PS
Sbjct: 20  PIPFHFPPMARAFLGSTPSSSLLTTSRLSSFTPS-LINNQRRRSLRLTTAAVVAMSEFPS 79

Query: 86  EKRIKPHPSLEVIGGGCDQFLPAFKDLDLPYKPFPVIGSNRHLETIFASFFRTCPSVNLH 145
           EK I+PHPSLEVIGGGCD+FLPA KDL+LPYKPFPVIG NRHLETIFASF+R+CP V   
Sbjct: 80  EKPIQPHPSLEVIGGGCDRFLPALKDLNLPYKPFPVIGWNRHLETIFASFYRSCPDVRFR 139

Query: 146 RECLRAADNGTVALDWVTGDDLRLPLDSPVLILLPGLTGGSQDSYVRHMLVRARDRGWRV 205
           RECLRAADNGTVALDWV GDDLRLP+D+PVLILLPGLTGGSQDSYVRHMLVRARDRGWR 
Sbjct: 140 RECLRAADNGTVALDWVVGDDLRLPIDAPVLILLPGLTGGSQDSYVRHMLVRARDRGWRA 199

Query: 206 VVFNSRGCGNSPVTTPQFYSASFLGDMREVVAHVTKRYPKANVYAVGWSLGGNILVNYLG 265
           VVFNSRGCGNSPVTTPQFYSASFLGDMREVV HVTKRYPKANVYAVGWSLGGNILVNYLG
Sbjct: 200 VVFNSRGCGNSPVTTPQFYSASFLGDMREVVEHVTKRYPKANVYAVGWSLGGNILVNYLG 259

Query: 266 QDSLTCPLSGAVSLCNPFDLIVADEDFRKGFNNVYDKSLARALRKIFNKHAALFTDMPSE 325
           QDSL+CPLSGAVSLCNPFDL+VADEDFRKGFNNVYDK+LARALR+IF+KHA LF DMP E
Sbjct: 260 QDSLSCPLSGAVSLCNPFDLVVADEDFRKGFNNVYDKALARALRRIFSKHADLFNDMPGE 319

Query: 326 FNIRLAAKARTVRDYDDALTRVSFGFNTVDEYYSKSSSSKFIKDVKIPLLCIQAANDPIA 385
           FNI LAA ARTVR+YDDALTRVSFGFN+VDEYYSKSSSSKFIKDVKIPLLCIQAANDPIA
Sbjct: 320 FNIPLAANARTVREYDDALTRVSFGFNSVDEYYSKSSSSKFIKDVKIPLLCIQAANDPIA 379

Query: 386 PNRGIPREDIKENPNCMLIVTPRGGHLGWVAGPGAPLGFPWTDPFVIDFLMYLENQKSKA 445
           P+RG+PREDIKENPNC+LIVTP+GGHLGWVAGP APLG PWTDPFVIDFL+YLE+Q+SKA
Sbjct: 380 PDRGVPREDIKENPNCLLIVTPKGGHLGWVAGPSAPLGNPWTDPFVIDFLVYLESQESKA 439

Query: 446 SASSTDLTTAEVM 459
           SASSTDLT AE +
Sbjct: 440 SASSTDLTAAETL 451

BLAST of Cmc09g0259141 vs. ExPASy Swiss-Prot
Match: Q40863 (Embryogenesis-associated protein EMB8 OS=Picea glauca OX=3330 GN=EMB8 PE=2 SV=1)

HSP 1 Score: 464.5 bits (1194), Expect = 1.4e-129
Identity = 243/418 (58.13%), Postives = 292/418 (69.86%), Query Frame = 0

Query: 49  IALRISSFTPSFFLTNQRPRSLRPTTGSVVAMAEVPS------EKRIKPHPSLEVIGGGC 108
           I+ RIS F   F          +P  G+     E+ S      ++R K  P  E+IG   
Sbjct: 25  ISRRISQFRAGF-------PDKKPAAGACEEQDELTSGSAARIQRRDKQLP--EIIGSD- 84

Query: 109 DQFLPAFKDLDLPYKPFPVIGSNRHLETIFASFFRTCPSVNLHRECLRAADNGTVALDW- 168
            + +     L  PY+ FP +G NRH+ETIFASFFR+ P +   RECLR  D GTV LDW 
Sbjct: 85  RELMSKLTTLGRPYRHFPFMG-NRHVETIFASFFRSWPVIKSRRECLRMEDGGTVELDWP 144

Query: 169 VTGDDL-----RLPLDSPVLILLPGLTGGSQDSYVRHMLVRARDRGWRVVVFNSRGCGNS 228
           + G+D       LP++SPVLILLPGLTGGS DSYV+HML+RAR  GW  VVFNSRGC +S
Sbjct: 145 LEGEDAELWNGELPVNSPVLILLPGLTGGSDDSYVKHMLLRARKHGWHSVVFNSRGCADS 204

Query: 229 PVTTPQFYSASFLGDMREVVAHVTKRYPKANVYAVGWSLGGNILVNYLGQDSLTCPLSGA 288
           PVTTPQFYSASF  D+ +VV HV  R+ ++N+YAVGWSLG NILV YLG+ +  CPLSGA
Sbjct: 205 PVTTPQFYSASFTKDLCQVVKHVAVRFSESNIYAVGWSLGANILVRYLGEVAGNCPLSGA 264

Query: 289 VSLCNPFDLIVADEDFRK--GFNNVYDKSLARALRKIFNKHAALFTDMPSEFNIRLAAKA 348
           VSLCNPF+L++ADEDF K  GFNNVYDK+LAR LR+IF KH  LF  +  E+NI   AKA
Sbjct: 265 VSLCNPFNLVIADEDFHKGLGFNNVYDKALARGLRQIFPKHTRLFEGIEGEYNIPTVAKA 324

Query: 349 RTVRDYDDALTRVSFGFNTVDEYYSKSSSSKFIKDVKIPLLCIQAANDPIAPNRGIPRED 408
           R+VRD+D  LTRVSFGF +V +YYS SSSS  IK V+  LLCIQA+NDPIAP+RGIP ED
Sbjct: 325 RSVRDFDGGLTRVSFGFQSVGDYYSNSSSSLSIKYVQTSLLCIQASNDPIAPSRGIPWED 384

Query: 409 IKENPNCMLIVTPRGGHLGWVAGPGAPLGFPWTDPFVIDFLMYLE-NQKSKASASSTD 452
           IKENPNC+L+VTP GGHLGWVAG  AP G PWTDP V+++L  LE NQ  K    + D
Sbjct: 385 IKENPNCLLVVTPNGGHLGWVAGDDAPFGAPWTDPLVMEYLEVLEKNQIEKPLRRTID 431

BLAST of Cmc09g0259141 vs. ExPASy Swiss-Prot
Match: Q0VC00 (Phospholipase ABHD3 OS=Bos taurus OX=9913 GN=ABHD3 PE=2 SV=1)

HSP 1 Score: 210.3 bits (534), Expect = 4.6e-53
Identity = 102/293 (34.81%), Postives = 167/293 (57.00%), Query Frame = 0

Query: 129 ETIFASFFRTCPSVNLHRECLRAADNGTVALDWVTGDDLRLPLDS---PVLILLPGLTGG 188
           +T+   F  + P V    E ++ AD G ++LDW   D+ +  +D+   P ++LLPGLTG 
Sbjct: 92  QTLLRPFITSKPLVQYRNELIKTADGGQISLDWFDNDNSKHYMDASTRPTVLLLPGLTGT 151

Query: 189 SQDSYVRHMLVRARDRGWRVVVFNSRGCGNSPVTTPQFYSASFLGDMREVVAHVTKRYPK 248
           S++SY+ HM+  + + G+R VVFN+RG     + TP+ Y  S   D+  V+ HV   YP 
Sbjct: 152 SKESYILHMIHLSEELGYRYVVFNNRGVAGENLLTPRTYCCSNTEDLETVIHHVHSLYPS 211

Query: 249 ANVYAVGWSLGGNILVNYLGQDSLTCPLSGAVSLCNPFDLIVADEDFRKGFN-NVYDKSL 308
           A   A G S+GG +L+NYLG+     PL  A +    ++     E   K  N  +++  L
Sbjct: 212 APFLAAGVSMGGMLLLNYLGKIGPKTPLKAAATFSVGWNTFACSESLEKPLNWLLFNYYL 271

Query: 309 ARALRKIFNKHAALFTDMPSEFNIRLAAKARTVRDYDDALTRVSFGFNTVDEYYSKSSSS 368
              L+   NKH  +F     + ++    KA+++R++D   T V FG+ T+D+YY+ +S +
Sbjct: 272 TTCLQSSVNKHRHMFV---KQIDVDHVMKAKSIREFDKRFTSVMFGYRTIDDYYTDASPN 331

Query: 369 KFIKDVKIPLLCIQAANDPIAPNRGIPREDIKENPNCMLIVTPRGGHLGWVAG 418
           + +K V IP+LC+ + +D  +P+  IP E  K+NPN  L++T  GGH+G++ G
Sbjct: 332 RRLKSVGIPVLCLNSVDDVFSPSHAIPIETAKQNPNVALVLTSYGGHIGFLEG 381

BLAST of Cmc09g0259141 vs. ExPASy Swiss-Prot
Match: Q8WU67 (Phospholipase ABHD3 OS=Homo sapiens OX=9606 GN=ABHD3 PE=2 SV=2)

HSP 1 Score: 208.4 bits (529), Expect = 1.7e-52
Identity = 102/293 (34.81%), Postives = 165/293 (56.31%), Query Frame = 0

Query: 129 ETIFASFFRTCPSVNLHRECLRAADNGTVALDWVTGDDLRLPLDS---PVLILLPGLTGG 188
           +T+   F  + P V    E ++ AD G ++LDW   D+    +D+   P ++LLPGLTG 
Sbjct: 92  QTLLRPFITSKPPVQYRNELIKTADGGQISLDWFDNDNSTCYMDASTRPTILLLPGLTGT 151

Query: 189 SQDSYVRHMLVRARDRGWRVVVFNSRGCGNSPVTTPQFYSASFLGDMREVVAHVTKRYPK 248
           S++SY+ HM+  + + G+R VVFN+RG     + TP+ Y  +   D+  V+ HV   YP 
Sbjct: 152 SKESYILHMIHLSEELGYRCVVFNNRGVAGENLLTPRTYCCANTEDLETVIHHVHSLYPS 211

Query: 249 ANVYAVGWSLGGNILVNYLGQDSLTCPLSGAVSLCNPFDLIVADEDFRKGFN-NVYDKSL 308
           A   A G S+GG +L+NYLG+     PL  A +    ++     E   K  N  +++  L
Sbjct: 212 APFLAAGVSMGGMLLLNYLGKIGSKTPLMAAATFSVGWNTFACSESLEKPLNWLLFNYYL 271

Query: 309 ARALRKIFNKHAALFTDMPSEFNIRLAAKARTVRDYDDALTRVSFGFNTVDEYYSKSSSS 368
              L+   NKH  +F     + ++    KA+++R++D   T V FG+ T+D+YY+ +S S
Sbjct: 272 TTCLQSSVNKHRHMFV---KQVDMDHVMKAKSIREFDKRFTSVMFGYQTIDDYYTDASPS 331

Query: 369 KFIKDVKIPLLCIQAANDPIAPNRGIPREDIKENPNCMLIVTPRGGHLGWVAG 418
             +K V IP+LC+ + +D  +P+  IP E  K+NPN  L++T  GGH+G++ G
Sbjct: 332 PRLKSVGIPVLCLNSVDDVFSPSHAIPIETAKQNPNVALVLTSYGGHIGFLEG 381

BLAST of Cmc09g0259141 vs. ExPASy Swiss-Prot
Match: Q91ZH7 (Phospholipase ABHD3 OS=Mus musculus OX=10090 GN=Abhd3 PE=1 SV=1)

HSP 1 Score: 204.1 bits (518), Expect = 3.3e-51
Identity = 100/293 (34.13%), Postives = 166/293 (56.66%), Query Frame = 0

Query: 129 ETIFASFFRTCPSVNLHRECLRAADNGTVALDWVTGDDLRLPLDS---PVLILLPGLTGG 188
           +T+   F  + P V    E ++ AD G ++LDW   ++    +D+   P ++LLPGLTG 
Sbjct: 92  QTLLRPFITSKPPVQYRNELIKTADGGQISLDWFDNNNSAYYVDASTRPTILLLPGLTGT 151

Query: 189 SQDSYVRHMLVRARDRGWRVVVFNSRGCGNSPVTTPQFYSASFLGDMREVVAHVTKRYPK 248
           S++SY+ HM+  + + G+R VVFN+RG     + TP+ Y  +   D+  VV HV   YP 
Sbjct: 152 SKESYILHMIHLSEELGYRCVVFNNRGVAGESLLTPRTYCCANTEDLEAVVHHVHSLYPG 211

Query: 249 ANVYAVGWSLGGNILVNYLGQDSLTCPLSGAVSLCNPFDLIVADEDFRKGFN-NVYDKSL 308
           A   A G S+GG +L+NYLG+     PL  A +    ++     E   +  N  +++  L
Sbjct: 212 APFLAAGVSMGGMLLLNYLGKIGSKTPLMAAATFSVGWNTFACSESLERPLNWLLFNYYL 271

Query: 309 ARALRKIFNKHAALFTDMPSEFNIRLAAKARTVRDYDDALTRVSFGFNTVDEYYSKSSSS 368
              L+    KH  +F +   + ++    KA+++R++D   T V FG+ T+D+YY+ +S +
Sbjct: 272 TTCLQSSVKKHRHMFVE---QIDMDQVMKAKSIREFDKRFTAVMFGYRTLDDYYTDASPN 331

Query: 369 KFIKDVKIPLLCIQAANDPIAPNRGIPREDIKENPNCMLIVTPRGGHLGWVAG 418
           + +K V IP+LC+ A +D  +P+  IP E  K+NPN  L++T  GGH+G++ G
Sbjct: 332 RRLKSVGIPVLCLNATDDVFSPSHAIPIETAKQNPNVALVLTAYGGHIGFLEG 381

BLAST of Cmc09g0259141 vs. ExPASy Swiss-Prot
Match: Q54H38 (Abhydrolase domain-containing protein OS=Dictyostelium discoideum OX=44689 GN=abhd PE=1 SV=1)

HSP 1 Score: 193.0 bits (489), Expect = 7.6e-48
Identity = 117/337 (34.72%), Postives = 184/337 (54.60%), Query Frame = 0

Query: 107 PAFKDLDLPYKPFPVIGSNRHLETIFASFFRTCPSVNL--HRECL-RAADNGTVALDWVT 166
           P  K+    Y P+ +   N H    + S+    P +NL   RE L    D GT++LD+  
Sbjct: 53  PTIKNGVTFYPPYYLY--NSHFMNYYGSY--KIPKLNLKTRREILVNPIDGGTISLDFFE 112

Query: 167 GDDLRLPLDSPVLILLPGLTGGSQDSYVRHMLVRA-RDRGWRVVVFNSRGCGNSPVTTPQ 226
             + +   D+P +++  GLTGGS + YV++   RA +++G+R VVFN RGC  +P+T  +
Sbjct: 113 LGEFK--EDTPTIVINHGLTGGSHERYVQYFAQRAYKEKGFRSVVFNYRGCAGNPITADR 172

Query: 227 FYSASFLGDMREVVAHVTK-RYPKANV-YAVGWSLGGNILVNYLGQDSLTCPLSGAVSLC 286
            YSA  L D++ V  ++TK   P     + VG+SLG  ILVNY+       P    VS+ 
Sbjct: 173 AYSAVQLDDIKFVTEYLTKTALPLVKKWFLVGFSLGSAILVNYMADAGKDSPYLAHVSIS 232

Query: 287 NPFDLIVADEDFRKGF--NNVYDKSLARALRKIFNKHAALFTDMPSEFNIRLAAKARTVR 346
           NP +++   ++    +  N +Y+K LA  L+++F K         ++  I     A+T+ 
Sbjct: 233 NPMNMVECTKNLSSTYINNLIYNKGLANNLKRLFRKFDGRLDKYATKEQI---MAAQTIA 292

Query: 347 DYDDALTRVSFGFNTVDEYYSKSSSSKFIKDVKIPLLCIQAANDPIAPNRGIPREDIKEN 406
           D+DD +T   FGF T  +YY  +SSSK I+++  P+L I A +DPIAP  G P +D K N
Sbjct: 293 DFDDLITSKMFGFETAHDYYLAASSSKSIRNLVKPILFINAIDDPIAPTSGFPWKDFKSN 352

Query: 407 PNCMLIVTPRGGHLGWVAGPGAPLGFPWTDPFVIDFL 436
           PN +L V+  GGHLG+++         W+D   +++L
Sbjct: 353 PNTILCVSRWGGHLGFISYED---HMSWSDKAAVEYL 377

BLAST of Cmc09g0259141 vs. ExPASy TrEMBL
Match: A0A5A7TMR0 (Embryogenesis-associated protein EMB8 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold376G001510 PE=4 SV=1)

HSP 1 Score: 939.1 bits (2426), Expect = 6.9e-270
Identity = 459/459 (100.00%), Postives = 459/459 (100.00%), Query Frame = 0

Query: 1   MQIQDDDMFMTLNNNHSRSSPISNIPIPSHSPPMARAFLGSTSSSSPLIALRISSFTPSF 60
           MQIQDDDMFMTLNNNHSRSSPISNIPIPSHSPPMARAFLGSTSSSSPLIALRISSFTPSF
Sbjct: 1   MQIQDDDMFMTLNNNHSRSSPISNIPIPSHSPPMARAFLGSTSSSSPLIALRISSFTPSF 60

Query: 61  FLTNQRPRSLRPTTGSVVAMAEVPSEKRIKPHPSLEVIGGGCDQFLPAFKDLDLPYKPFP 120
           FLTNQRPRSLRPTTGSVVAMAEVPSEKRIKPHPSLEVIGGGCDQFLPAFKDLDLPYKPFP
Sbjct: 61  FLTNQRPRSLRPTTGSVVAMAEVPSEKRIKPHPSLEVIGGGCDQFLPAFKDLDLPYKPFP 120

Query: 121 VIGSNRHLETIFASFFRTCPSVNLHRECLRAADNGTVALDWVTGDDLRLPLDSPVLILLP 180
           VIGSNRHLETIFASFFRTCPSVNLHRECLRAADNGTVALDWVTGDDLRLPLDSPVLILLP
Sbjct: 121 VIGSNRHLETIFASFFRTCPSVNLHRECLRAADNGTVALDWVTGDDLRLPLDSPVLILLP 180

Query: 181 GLTGGSQDSYVRHMLVRARDRGWRVVVFNSRGCGNSPVTTPQFYSASFLGDMREVVAHVT 240
           GLTGGSQDSYVRHMLVRARDRGWRVVVFNSRGCGNSPVTTPQFYSASFLGDMREVVAHVT
Sbjct: 181 GLTGGSQDSYVRHMLVRARDRGWRVVVFNSRGCGNSPVTTPQFYSASFLGDMREVVAHVT 240

Query: 241 KRYPKANVYAVGWSLGGNILVNYLGQDSLTCPLSGAVSLCNPFDLIVADEDFRKGFNNVY 300
           KRYPKANVYAVGWSLGGNILVNYLGQDSLTCPLSGAVSLCNPFDLIVADEDFRKGFNNVY
Sbjct: 241 KRYPKANVYAVGWSLGGNILVNYLGQDSLTCPLSGAVSLCNPFDLIVADEDFRKGFNNVY 300

Query: 301 DKSLARALRKIFNKHAALFTDMPSEFNIRLAAKARTVRDYDDALTRVSFGFNTVDEYYSK 360
           DKSLARALRKIFNKHAALFTDMPSEFNIRLAAKARTVRDYDDALTRVSFGFNTVDEYYSK
Sbjct: 301 DKSLARALRKIFNKHAALFTDMPSEFNIRLAAKARTVRDYDDALTRVSFGFNTVDEYYSK 360

Query: 361 SSSSKFIKDVKIPLLCIQAANDPIAPNRGIPREDIKENPNCMLIVTPRGGHLGWVAGPGA 420
           SSSSKFIKDVKIPLLCIQAANDPIAPNRGIPREDIKENPNCMLIVTPRGGHLGWVAGPGA
Sbjct: 361 SSSSKFIKDVKIPLLCIQAANDPIAPNRGIPREDIKENPNCMLIVTPRGGHLGWVAGPGA 420

Query: 421 PLGFPWTDPFVIDFLMYLENQKSKASASSTDLTTAEVMT 460
           PLGFPWTDPFVIDFLMYLENQKSKASASSTDLTTAEVMT
Sbjct: 421 PLGFPWTDPFVIDFLMYLENQKSKASASSTDLTTAEVMT 459

BLAST of Cmc09g0259141 vs. ExPASy TrEMBL
Match: A0A1S3AU57 (LOW QUALITY PROTEIN: embryogenesis-associated protein EMB8 OS=Cucumis melo OX=3656 GN=LOC103482859 PE=4 SV=1)

HSP 1 Score: 925.6 bits (2391), Expect = 7.9e-266
Identity = 452/452 (100.00%), Postives = 452/452 (100.00%), Query Frame = 0

Query: 8   MFMTLNNNHSRSSPISNIPIPSHSPPMARAFLGSTSSSSPLIALRISSFTPSFFLTNQRP 67
           MFMTLNNNHSRSSPISNIPIPSHSPPMARAFLGSTSSSSPLIALRISSFTPSFFLTNQRP
Sbjct: 1   MFMTLNNNHSRSSPISNIPIPSHSPPMARAFLGSTSSSSPLIALRISSFTPSFFLTNQRP 60

Query: 68  RSLRPTTGSVVAMAEVPSEKRIKPHPSLEVIGGGCDQFLPAFKDLDLPYKPFPVIGSNRH 127
           RSLRPTTGSVVAMAEVPSEKRIKPHPSLEVIGGGCDQFLPAFKDLDLPYKPFPVIGSNRH
Sbjct: 61  RSLRPTTGSVVAMAEVPSEKRIKPHPSLEVIGGGCDQFLPAFKDLDLPYKPFPVIGSNRH 120

Query: 128 LETIFASFFRTCPSVNLHRECLRAADNGTVALDWVTGDDLRLPLDSPVLILLPGLTGGSQ 187
           LETIFASFFRTCPSVNLHRECLRAADNGTVALDWVTGDDLRLPLDSPVLILLPGLTGGSQ
Sbjct: 121 LETIFASFFRTCPSVNLHRECLRAADNGTVALDWVTGDDLRLPLDSPVLILLPGLTGGSQ 180

Query: 188 DSYVRHMLVRARDRGWRVVVFNSRGCGNSPVTTPQFYSASFLGDMREVVAHVTKRYPKAN 247
           DSYVRHMLVRARDRGWRVVVFNSRGCGNSPVTTPQFYSASFLGDMREVVAHVTKRYPKAN
Sbjct: 181 DSYVRHMLVRARDRGWRVVVFNSRGCGNSPVTTPQFYSASFLGDMREVVAHVTKRYPKAN 240

Query: 248 VYAVGWSLGGNILVNYLGQDSLTCPLSGAVSLCNPFDLIVADEDFRKGFNNVYDKSLARA 307
           VYAVGWSLGGNILVNYLGQDSLTCPLSGAVSLCNPFDLIVADEDFRKGFNNVYDKSLARA
Sbjct: 241 VYAVGWSLGGNILVNYLGQDSLTCPLSGAVSLCNPFDLIVADEDFRKGFNNVYDKSLARA 300

Query: 308 LRKIFNKHAALFTDMPSEFNIRLAAKARTVRDYDDALTRVSFGFNTVDEYYSKSSSSKFI 367
           LRKIFNKHAALFTDMPSEFNIRLAAKARTVRDYDDALTRVSFGFNTVDEYYSKSSSSKFI
Sbjct: 301 LRKIFNKHAALFTDMPSEFNIRLAAKARTVRDYDDALTRVSFGFNTVDEYYSKSSSSKFI 360

Query: 368 KDVKIPLLCIQAANDPIAPNRGIPREDIKENPNCMLIVTPRGGHLGWVAGPGAPLGFPWT 427
           KDVKIPLLCIQAANDPIAPNRGIPREDIKENPNCMLIVTPRGGHLGWVAGPGAPLGFPWT
Sbjct: 361 KDVKIPLLCIQAANDPIAPNRGIPREDIKENPNCMLIVTPRGGHLGWVAGPGAPLGFPWT 420

Query: 428 DPFVIDFLMYLENQKSKASASSTDLTTAEVMT 460
           DPFVIDFLMYLENQKSKASASSTDLTTAEVMT
Sbjct: 421 DPFVIDFLMYLENQKSKASASSTDLTTAEVMT 452

BLAST of Cmc09g0259141 vs. ExPASy TrEMBL
Match: A0A0A0KNN2 (AB hydrolase-1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G148530 PE=4 SV=1)

HSP 1 Score: 888.3 bits (2294), Expect = 1.4e-254
Identity = 433/459 (94.34%), Postives = 444/459 (96.73%), Query Frame = 0

Query: 1   MQIQDDDMFMTLNNNHSRSSPISNIPIPSHSPPMARAFLGSTSSSSPLIALRISSFTPSF 60
           +QIQDD +   LNNNHSRSSPISNIPIPSHSPPMARAFLGST SSSPLIALR+SSFTPSF
Sbjct: 22  IQIQDDGIIRILNNNHSRSSPISNIPIPSHSPPMARAFLGSTPSSSPLIALRLSSFTPSF 81

Query: 61  FLTNQRPRSLRPTTGSVVAMAEVPSEKRIKPHPSLEVIGGGCDQFLPAFKDLDLPYKPFP 120
           FLTNQRPRSLR TT +VV MAE+PSEKRIKPHPSLEVIGGGCDQFLPAFKDLDLPYKPFP
Sbjct: 82  FLTNQRPRSLRLTTDTVVPMAELPSEKRIKPHPSLEVIGGGCDQFLPAFKDLDLPYKPFP 141

Query: 121 VIGSNRHLETIFASFFRTCPSVNLHRECLRAADNGTVALDWVTGDDLRLPLDSPVLILLP 180
           VIGSNRHLETIFASFFRTCPSVNLHRECLRAADNGTVALDWV GDDLRLPLDSPVLILLP
Sbjct: 142 VIGSNRHLETIFASFFRTCPSVNLHRECLRAADNGTVALDWVAGDDLRLPLDSPVLILLP 201

Query: 181 GLTGGSQDSYVRHMLVRARDRGWRVVVFNSRGCGNSPVTTPQFYSASFLGDMREVVAHVT 240
           GLTGGSQDSYVRHMLVRARDRGWRVVVFNSRGCGNSPVTTPQFYSASFLGDMREVVAHVT
Sbjct: 202 GLTGGSQDSYVRHMLVRARDRGWRVVVFNSRGCGNSPVTTPQFYSASFLGDMREVVAHVT 261

Query: 241 KRYPKANVYAVGWSLGGNILVNYLGQDSLTCPLSGAVSLCNPFDLIVADEDFRKGFNNVY 300
           +RYPKAN+YAVGWSLGGNILVNYLGQDSLTCPLSGAVSLCNPFDLIVADEDFRKGFNNVY
Sbjct: 262 ERYPKANLYAVGWSLGGNILVNYLGQDSLTCPLSGAVSLCNPFDLIVADEDFRKGFNNVY 321

Query: 301 DKSLARALRKIFNKHAALFTDMPSEFNIRLAAKARTVRDYDDALTRVSFGFNTVDEYYSK 360
           DKSLA ALRKIFNKHAALFTDM  EFNIRLAA+A TVRDYDDALTRVSFGFNTVDEYYSK
Sbjct: 322 DKSLAGALRKIFNKHAALFTDMTGEFNIRLAAQATTVRDYDDALTRVSFGFNTVDEYYSK 381

Query: 361 SSSSKFIKDVKIPLLCIQAANDPIAPNRGIPREDIKENPNCMLIVTPRGGHLGWVAGPGA 420
           SSSSKFIKDVKIPLLCIQAANDPIAPNRGIPREDI+ENPNCMLIVTP+GGHLGWVAGPGA
Sbjct: 382 SSSSKFIKDVKIPLLCIQAANDPIAPNRGIPREDIEENPNCMLIVTPKGGHLGWVAGPGA 441

Query: 421 PLGFPWTDPFVIDFLMYLENQKSKASASSTDLTTAEVMT 460
           PLGFPWTDPFVIDFLMYLEN+K KASASSTDLT AEVMT
Sbjct: 442 PLGFPWTDPFVIDFLMYLENKKFKASASSTDLTMAEVMT 480

BLAST of Cmc09g0259141 vs. ExPASy TrEMBL
Match: A0A6J1GD89 (embryogenesis-associated protein EMB8-like OS=Cucurbita moschata OX=3662 GN=LOC111453123 PE=4 SV=1)

HSP 1 Score: 775.8 bits (2002), Expect = 1.0e-220
Identity = 374/433 (86.37%), Postives = 400/433 (92.38%), Query Frame = 0

Query: 26  PIPSHSPPMARAFLGSTSSSSPLIALRISSFTPSFFLTNQRPRSLRPTTGSVVAMAEVPS 85
           PIP H PPMARAFLGST SSS L   R+SSFTPS  + NQR RSLR TT +VVAM+E PS
Sbjct: 20  PIPFHFPPMARAFLGSTPSSSLLTTSRLSSFTPS-LINNQRRRSLRLTTAAVVAMSEFPS 79

Query: 86  EKRIKPHPSLEVIGGGCDQFLPAFKDLDLPYKPFPVIGSNRHLETIFASFFRTCPSVNLH 145
           EK I+PHPSLEVIGGGCD+FLPA KDL+LPYKPFPVIG NRHLETIFASF+R+CP V   
Sbjct: 80  EKPIQPHPSLEVIGGGCDRFLPALKDLNLPYKPFPVIGWNRHLETIFASFYRSCPDVRFR 139

Query: 146 RECLRAADNGTVALDWVTGDDLRLPLDSPVLILLPGLTGGSQDSYVRHMLVRARDRGWRV 205
           RECLRAADNGTVALDWV GDDLRLP+D+PVLILLPGLTGGSQDSYVRHMLVRARDRGWR 
Sbjct: 140 RECLRAADNGTVALDWVVGDDLRLPIDAPVLILLPGLTGGSQDSYVRHMLVRARDRGWRA 199

Query: 206 VVFNSRGCGNSPVTTPQFYSASFLGDMREVVAHVTKRYPKANVYAVGWSLGGNILVNYLG 265
           VVFNSRGCGNSPVTTPQFYSASFLGDMREVV HVTKRYPKANVYAVGWSLGGNILVNYLG
Sbjct: 200 VVFNSRGCGNSPVTTPQFYSASFLGDMREVVEHVTKRYPKANVYAVGWSLGGNILVNYLG 259

Query: 266 QDSLTCPLSGAVSLCNPFDLIVADEDFRKGFNNVYDKSLARALRKIFNKHAALFTDMPSE 325
           QDSL+CPLSGAVSLCNPFDL+VADEDFRKGFNNVYDK+LARALR+IF+KHA LF DMP E
Sbjct: 260 QDSLSCPLSGAVSLCNPFDLVVADEDFRKGFNNVYDKALARALRRIFSKHADLFNDMPGE 319

Query: 326 FNIRLAAKARTVRDYDDALTRVSFGFNTVDEYYSKSSSSKFIKDVKIPLLCIQAANDPIA 385
           FNI LAA ARTVR+YDDALTRVSFGFN+VDEYYSKSSSSKFIKDVKIPLLCIQAANDPIA
Sbjct: 320 FNIPLAANARTVREYDDALTRVSFGFNSVDEYYSKSSSSKFIKDVKIPLLCIQAANDPIA 379

Query: 386 PNRGIPREDIKENPNCMLIVTPRGGHLGWVAGPGAPLGFPWTDPFVIDFLMYLENQKSKA 445
           P+RG+PREDIKENPNC+LIVTP+GGHLGWVAGP APLG PWTDPFVIDFL+YLE+Q+SKA
Sbjct: 380 PDRGVPREDIKENPNCLLIVTPKGGHLGWVAGPSAPLGNPWTDPFVIDFLVYLESQESKA 439

Query: 446 SASSTDLTTAEVM 459
           SASSTDLT AE +
Sbjct: 440 SASSTDLTAAETL 451

BLAST of Cmc09g0259141 vs. ExPASy TrEMBL
Match: A0A6J1IJD9 (embryogenesis-associated protein EMB8-like OS=Cucurbita maxima OX=3661 GN=LOC111477973 PE=4 SV=1)

HSP 1 Score: 770.8 bits (1989), Expect = 3.3e-219
Identity = 373/433 (86.14%), Postives = 399/433 (92.15%), Query Frame = 0

Query: 26  PIPSHSPPMARAFLGSTSSSSPLIALRISSFTPSFFLTNQRPRSLRPTTGSVVAMAEVPS 85
           PIP H PPMARAFLGST SSS L   R+SSFTPS  +TNQR RSLR TT +VVAM+E PS
Sbjct: 20  PIPFHFPPMARAFLGSTPSSSLLTTSRLSSFTPS-LITNQRRRSLRLTTAAVVAMSEFPS 79

Query: 86  EKRIKPHPSLEVIGGGCDQFLPAFKDLDLPYKPFPVIGSNRHLETIFASFFRTCPSVNLH 145
           EK I+PHPSLEVIGGG D+FLPA KDL+LPYKPFPVIG NRHLETIFASF+R+CP V   
Sbjct: 80  EKPIQPHPSLEVIGGGRDRFLPALKDLNLPYKPFPVIGWNRHLETIFASFYRSCPDVRFR 139

Query: 146 RECLRAADNGTVALDWVTGDDLRLPLDSPVLILLPGLTGGSQDSYVRHMLVRARDRGWRV 205
           RECLRAADNGTVALDWV GDDLRLP+D+PVLILLPGLTGGSQDSYVRHMLVRARDRGWR 
Sbjct: 140 RECLRAADNGTVALDWVVGDDLRLPIDAPVLILLPGLTGGSQDSYVRHMLVRARDRGWRA 199

Query: 206 VVFNSRGCGNSPVTTPQFYSASFLGDMREVVAHVTKRYPKANVYAVGWSLGGNILVNYLG 265
           VVFNSRGCGNSPVTTPQFYSASFLGDMREVV HVTKRYPKANVYAVGWSLGGNILVNYLG
Sbjct: 200 VVFNSRGCGNSPVTTPQFYSASFLGDMREVVEHVTKRYPKANVYAVGWSLGGNILVNYLG 259

Query: 266 QDSLTCPLSGAVSLCNPFDLIVADEDFRKGFNNVYDKSLARALRKIFNKHAALFTDMPSE 325
           QDSL+CPLSGAVSLCNPFDL+VADEDFRKGFNNVYDK+LARALR+IF+KHA LF DMP E
Sbjct: 260 QDSLSCPLSGAVSLCNPFDLVVADEDFRKGFNNVYDKALARALRRIFSKHADLFNDMPGE 319

Query: 326 FNIRLAAKARTVRDYDDALTRVSFGFNTVDEYYSKSSSSKFIKDVKIPLLCIQAANDPIA 385
           FNI LAA ARTVR+YDDALTRVSFGFN+VDEYYSKSSSSKFIKDVKIPLLCIQAANDPIA
Sbjct: 320 FNIPLAANARTVREYDDALTRVSFGFNSVDEYYSKSSSSKFIKDVKIPLLCIQAANDPIA 379

Query: 386 PNRGIPREDIKENPNCMLIVTPRGGHLGWVAGPGAPLGFPWTDPFVIDFLMYLENQKSKA 445
           P+RG+PREDIK NPNC+LIVTP+GGHLGWVAGP APLG PWTDPFVIDFL+YLE+Q+SKA
Sbjct: 380 PDRGVPREDIKANPNCLLIVTPKGGHLGWVAGPSAPLGNPWTDPFVIDFLVYLESQESKA 439

Query: 446 SASSTDLTTAEVM 459
           SASSTDLT AE +
Sbjct: 440 SASSTDLTAAETL 451

BLAST of Cmc09g0259141 vs. TAIR 10
Match: AT3G50790.1 (esterase/lipase/thioesterase family protein )

HSP 1 Score: 561.2 bits (1445), Expect = 7.6e-160
Identity = 265/369 (71.82%), Postives = 307/369 (83.20%), Query Frame = 0

Query: 76  SVVAMAEVPSEKRIKPHPSLEVIGGGCDQFLPAFKD-LDLPYKPFPVIGSNRHLETIFAS 135
           SV A A  P   R   H SLEVIGGG D+FLP+ KD L  PY  FP+IG NRH+ETI+AS
Sbjct: 25  SVFAAAN-PEMSRPSHHSSLEVIGGGSDRFLPSLKDSLAKPYNAFPLIGFNRHVETIYAS 84

Query: 136 FFRTCPSVNLHRECLRAADNGTVALDWVTGDDLRLPLDSPVLILLPGLTGGSQDSYVRHM 195
           FFR+ P V L RECLR  DNG+VALDWV G+D   P DSP+LILLPGLTGGSQDSYVRHM
Sbjct: 85  FFRSVPFVRLRRECLRTKDNGSVALDWVAGEDRHFPPDSPILILLPGLTGGSQDSYVRHM 144

Query: 196 LVRARDRGWRVVVFNSRGCGNSPVTTPQFYSASFLGDMREVVAHVTKRYPKANVYAVGWS 255
           L+RA+ + WR VVFNSRGCG+SPVTTPQFYSASFLGD+ EV+ HV  ++PKAN+YA GWS
Sbjct: 145 LLRAQSKKWRCVVFNSRGCGDSPVTTPQFYSASFLGDIGEVIDHVVDKFPKANLYAAGWS 204

Query: 256 LGGNILVNYLGQDSLTCPLSGAVSLCNPFDLIVADEDFRKGFNNVYDKSLARALRKIFNK 315
           LGGNILVNYLGQ+S  CPL+ AVSLCNPFDL++ADEDF KGFNNVYDK+L+++LR+IF+K
Sbjct: 205 LGGNILVNYLGQESHNCPLTAAVSLCNPFDLVIADEDFHKGFNNVYDKALSKSLRRIFSK 264

Query: 316 HAALFTDMPSEFNIRLAAKARTVRDYDDALTRVSFGFNTVDEYYSKSSSSKFIKDVKIPL 375
           H+ LF D+  EFNI LAA A TVRD+DD LTRVSFGF +VDEYYSKSSSSK IK V+IPL
Sbjct: 265 HSLLFEDIGGEFNIPLAANAETVRDFDDGLTRVSFGFKSVDEYYSKSSSSKAIKHVRIPL 324

Query: 376 LCIQAANDPIAPNRGIPREDIKENPNCMLIVTPRGGHLGWVAGPGAPLGFPWTDPFVIDF 435
           LCIQAANDPIAP RGIPR+DIK NPNC+LIVTPRGGHLGWVAG  AP G PWTD  V++F
Sbjct: 325 LCIQAANDPIAPERGIPRDDIKANPNCVLIVTPRGGHLGWVAGEEAPNGAPWTDQVVMEF 384

Query: 436 LMYLENQKS 444
           L ++EN+++
Sbjct: 385 LQHVENRET 392

BLAST of Cmc09g0259141 vs. TAIR 10
Match: AT5G49950.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 227.6 bits (579), Expect = 2.0e-59
Identity = 112/307 (36.48%), Postives = 178/307 (57.98%), Query Frame = 0

Query: 124 SNRHLETIFASFFRTCPSVNLHRECLRAADNGTVALDWVTGDDLRLPLD----------- 183
           S+ HL+T F + F   P  +  R   +A D GT+ALDW+   D+   +            
Sbjct: 87  SSPHLQTAFLTLFGKSPPFSYKRILYQATDGGTIALDWLMHSDVVEGISQVVNASNPGTD 146

Query: 184 -SPVLILLPGLTGGSQDSYVRHMLVRARDRGWRVVVFNSRGCGNSPVTTPQFYSASFLGD 243
            +P+ I++PGLT  S  +Y++H+  R    GW VVV N RG G   +T+   Y+A +  D
Sbjct: 147 RTPIAIIVPGLTSDSSAAYIKHIAFRLAKEGWNVVVQNHRGLGGISLTSDCVYTAGWTED 206

Query: 244 MREVVAHVTKRYPKANVYAVGWSLGGNILVNYLGQDSLTCPLSGAVSLCNPFDLIVADED 303
           +R+V+AH+  ++P+A ++AVG S+G N+LV YLG+D    PL GA ++C+P+DL++ D  
Sbjct: 207 LRKVIAHIHSQFPEAPLFAVGTSIGANVLVKYLGEDGPNTPLIGATAVCSPWDLLICDRF 266

Query: 304 F-RKGFNNVYDKSLARALRKIFNKHAALFTDMPSEFNIRLAAKARTVRDYDDALTRVSFG 363
             RK    VYD+ L   L+     H ++ + +     I+   K+R+VR++D+  TR+   
Sbjct: 267 INRKLVQKVYDRMLTIGLQGYAQLHHSIISRIADWEGIK---KSRSVREFDNYATRLVAK 326

Query: 364 FNTVDEYYSKSSSSKFIKDVKIPLLCIQAANDPIAPNRGIPREDIKENPNCMLIVTPRGG 418
           F T D YY +SSSS+++++V +PLLCI A +DP+     IP ++ + N N +L  T  GG
Sbjct: 327 FETTDTYYRRSSSSQYVENVAVPLLCISALDDPVCTREAIPWDECRANKNIVLATTTHGG 386

BLAST of Cmc09g0259141 vs. TAIR 10
Match: AT1G34340.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 216.9 bits (551), Expect = 3.5e-56
Identity = 109/306 (35.62%), Postives = 174/306 (56.86%), Query Frame = 0

Query: 127 HLETIFASFFRTCPSVNLHRECLRAADNGTVALDWVTGDDLRLPLD-------------- 186
           H++T F +F    P     R+    +D GT+ALDW+T  D+   LD              
Sbjct: 96  HIQTCFLNFHGLPPVFTYTRQLFLTSDGGTIALDWLTNSDV---LDGSLHNKSEITKEDT 155

Query: 187 SPVLILLPGLTGGSQDSYVRHMLVRARDRGWRVVVFNSRGCGNSPVTTPQFYSASFLGDM 246
           +P+ +++PGLT  S  +Y++H+       GW VV+ N RG G   VT+  FY+A +  D+
Sbjct: 156 TPIAVVIPGLTSDSSSAYLKHLAYDTAKTGWNVVISNHRGLGGVSVTSDCFYNAGWTDDI 215

Query: 247 REVVAHVTKRYPKANVYAVGWSLGGNILVNYLGQDSLTCPLSGAVSLCNPFDLIVADEDF 306
           R V+ ++  +YP+A ++A+G S+G N+LV YLG++    PL GAV++C+P+DL++ D   
Sbjct: 216 RVVLDYLQHKYPRAPLFAIGTSIGANVLVKYLGEEGEKTPLRGAVAICSPWDLLIGDRFI 275

Query: 307 -RKGFNNVYDKSLARALRKIFNKHAALFTDMPSEFNIRLAAKARTVRDYDDALTRVSFGF 366
            R     +YDK+L   L+     H   F  + +   I+   K+R++RD+D+  T +   F
Sbjct: 276 CRTLKQKLYDKALTIGLQGYAQLHEPQFLRLANWEGIK---KSRSIRDFDNHATCLVGKF 335

Query: 367 NTVDEYYSKSSSSKFIKDVKIPLLCIQAANDPIAPNRGIPREDIKENPNCMLIVTPRGGH 418
            TVD YY KSSS++++ +V +PLLCI A +DP+     IP ++ + N N +L  T  GGH
Sbjct: 336 ETVDTYYRKSSSTQYVGNVAVPLLCISALDDPLCTKEAIPWDECRANKNIVLATTNHGGH 395

BLAST of Cmc09g0259141 vs. TAIR 10
Match: AT2G03140.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 159.8 bits (403), Expect = 5.1e-39
Identity = 93/263 (35.36%), Postives = 143/263 (54.37%), Query Frame = 0

Query: 146 RECLRAADNGTVALDWVTGDDLR--LPLDSPVLILLPGLTGGSQDSYVRHMLVRARDRGW 205
           R C+   D G V+LDW    D+R    LD+ V + +PG   GS +  VR  +  A  RG 
Sbjct: 172 RVCITMEDGGVVSLDWPANLDIREERGLDTTV-VFIPGTPEGSMEEGVRSFVCEALRRGV 231

Query: 206 RVVVFNSRGCGNSPVTTPQFYSASFLGDMREVVAHVTKRYPKANVYAVGWSLGGNILVNY 265
             VV N RGC  SP+TTP+ ++A    D+   +  ++K  P   + AVG   G N+L  Y
Sbjct: 232 FPVVMNPRGCAGSPLTTPRLFTAGDSDDISTALRFLSKTRPWTTLTAVGRGYGANMLTKY 291

Query: 266 LGQDSLTCPLSGAVSLCNPFDLIVADEDFRKG-FNNVYDKSLARALRKIFNKHAALFTDM 325
           L +     PL+ AV + NPFDL   +E  R   ++   D+ L R L +I   +  LF   
Sbjct: 292 LAEAGERTPLTAAVCIDNPFDL---EEITRTSPYSTSLDQQLTRGLVEILLANKELFQGR 351

Query: 326 PSEFNIRLAAKARTVRDYDDALTRVSFGFNTVDEYYSKSSSSKFIKDVKIPLLCIQAAND 385
              F++  A  +++VR++D AL+ V++G  +++++YS  ++ + I +VK+PLL IQ  ND
Sbjct: 352 AKAFDVGKALCSKSVREFDKALSMVTYGCESIEDFYSSCATREVIGEVKVPLLFIQ--ND 411

Query: 386 PIAPNRGIPREDIKENPNCMLIV 406
            + P   IPR  I ENP   L++
Sbjct: 412 DVVPPYTIPRSSIAENPFTSLLL 428

BLAST of Cmc09g0259141 vs. TAIR 10
Match: AT2G03140.2 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 159.8 bits (403), Expect = 5.1e-39
Identity = 93/263 (35.36%), Postives = 143/263 (54.37%), Query Frame = 0

Query: 146 RECLRAADNGTVALDWVTGDDLR--LPLDSPVLILLPGLTGGSQDSYVRHMLVRARDRGW 205
           R C+   D G V+LDW    D+R    LD+ V + +PG   GS +  VR  +  A  RG 
Sbjct: 172 RVCITMEDGGVVSLDWPANLDIREERGLDTTV-VFIPGTPEGSMEEGVRSFVCEALRRGV 231

Query: 206 RVVVFNSRGCGNSPVTTPQFYSASFLGDMREVVAHVTKRYPKANVYAVGWSLGGNILVNY 265
             VV N RGC  SP+TTP+ ++A    D+   +  ++K  P   + AVG   G N+L  Y
Sbjct: 232 FPVVMNPRGCAGSPLTTPRLFTAGDSDDISTALRFLSKTRPWTTLTAVGRGYGANMLTKY 291

Query: 266 LGQDSLTCPLSGAVSLCNPFDLIVADEDFRKG-FNNVYDKSLARALRKIFNKHAALFTDM 325
           L +     PL+ AV + NPFDL   +E  R   ++   D+ L R L +I   +  LF   
Sbjct: 292 LAEAGERTPLTAAVCIDNPFDL---EEITRTSPYSTSLDQQLTRGLVEILLANKELFQGR 351

Query: 326 PSEFNIRLAAKARTVRDYDDALTRVSFGFNTVDEYYSKSSSSKFIKDVKIPLLCIQAAND 385
              F++  A  +++VR++D AL+ V++G  +++++YS  ++ + I +VK+PLL IQ  ND
Sbjct: 352 AKAFDVGKALCSKSVREFDKALSMVTYGCESIEDFYSSCATREVIGEVKVPLLFIQ--ND 411

Query: 386 PIAPNRGIPREDIKENPNCMLIV 406
            + P   IPR  I ENP   L++
Sbjct: 412 DVVPPYTIPRSSIAENPFTSLLL 428

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAA0042649.11.4e-269100.00embryogenesis-associated protein EMB8 [Cucumis melo var. makuwa] >TYK06050.1 emb... [more]
XP_008437445.11.6e-265100.00PREDICTED: LOW QUALITY PROTEIN: embryogenesis-associated protein EMB8 [Cucumis m... [more]
XP_004143840.31.0e-25494.55embryogenesis-associated protein EMB8 [Cucumis sativus] >KGN49619.2 hypothetical... [more]
XP_038875981.16.7e-22790.85embryogenesis-associated protein EMB8 [Benincasa hispida][more]
XP_022949852.12.1e-22086.37embryogenesis-associated protein EMB8-like [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
Q408631.4e-12958.13Embryogenesis-associated protein EMB8 OS=Picea glauca OX=3330 GN=EMB8 PE=2 SV=1[more]
Q0VC004.6e-5334.81Phospholipase ABHD3 OS=Bos taurus OX=9913 GN=ABHD3 PE=2 SV=1[more]
Q8WU671.7e-5234.81Phospholipase ABHD3 OS=Homo sapiens OX=9606 GN=ABHD3 PE=2 SV=2[more]
Q91ZH73.3e-5134.13Phospholipase ABHD3 OS=Mus musculus OX=10090 GN=Abhd3 PE=1 SV=1[more]
Q54H387.6e-4834.72Abhydrolase domain-containing protein OS=Dictyostelium discoideum OX=44689 GN=ab... [more]
Match NameE-valueIdentityDescription
A0A5A7TMR06.9e-270100.00Embryogenesis-associated protein EMB8 OS=Cucumis melo var. makuwa OX=1194695 GN=... [more]
A0A1S3AU577.9e-266100.00LOW QUALITY PROTEIN: embryogenesis-associated protein EMB8 OS=Cucumis melo OX=36... [more]
A0A0A0KNN21.4e-25494.34AB hydrolase-1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G148... [more]
A0A6J1GD891.0e-22086.37embryogenesis-associated protein EMB8-like OS=Cucurbita moschata OX=3662 GN=LOC1... [more]
A0A6J1IJD93.3e-21986.14embryogenesis-associated protein EMB8-like OS=Cucurbita maxima OX=3661 GN=LOC111... [more]
Match NameE-valueIdentityDescription
AT3G50790.17.6e-16071.82esterase/lipase/thioesterase family protein [more]
AT5G49950.12.0e-5936.48alpha/beta-Hydrolases superfamily protein [more]
AT1G34340.13.5e-5635.62alpha/beta-Hydrolases superfamily protein [more]
AT2G03140.15.1e-3935.36alpha/beta-Hydrolases superfamily protein [more]
AT2G03140.25.1e-3935.36alpha/beta-Hydrolases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Melon (Charmono) v1.1
Date Performed: 2022-10-13
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000073Alpha/beta hydrolase fold-1PFAMPF00561Abhydrolase_1coord: 174..412
e-value: 2.9E-20
score: 73.0
IPR029058Alpha/Beta hydrolase foldGENE3D3.40.50.1820alpha/beta hydrolasecoord: 162..416
e-value: 5.3E-26
score: 93.5
IPR029058Alpha/Beta hydrolase foldSUPERFAMILY53474alpha/beta-Hydrolasescoord: 142..421
NoneNo IPR availablePANTHERPTHR10794ABHYDROLASE DOMAIN-CONTAINING PROTEINcoord: 68..449
NoneNo IPR availablePANTHERPTHR10794:SF81EMBRYOGENESIS-ASSOCIATED PROTEIN EMB8-LIKEcoord: 68..449
IPR000952AB hydrolase 4, conserved sitePROSITEPS01133UPF0017coord: 341..382

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cmc09g0259141.1Cmc09g0259141.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0044255 cellular lipid metabolic process
molecular_function GO:0047372 acylglycerol lipase activity
molecular_function GO:0034338 short-chain carboxylesterase activity