Cmc09g0258291 (gene) Melon (Charmono) v1.1

Overview
NameCmc09g0258291
Typegene
OrganismCucumis melo L. var. cantalupensis cv. Charmono (Melon (Charmono) v1.1)
Descriptionmeiotic recombination protein SPO11-2
LocationCMiso1.1chr09: 22449096 .. 22452974 (-)
RNA-Seq ExpressionCmc09g0258291
SyntenyCmc09g0258291
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TGGTGATCTCTAACGTGACGAAGGGTAAGCGCGGGATGCAACGGAATCGAAGGTCATCCGTAAGAAACCCGAGGTATAAATTAAAGGGGAGAGCCTCGCCTAGCCCAATGCTTTTTGCAACACTGTCCCATTTTTGGTTTGGAGGGAGTTGTGGACTTGATCATGAAATTGTATCATCAACGTACTCGCAAAAGAAAATGATCTTTACAATGGAACAATCTATAGGCGCTTTGATTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTTCTCTCTCTAGATTTCGAACAACCAACATATAATCAGCGGCATTTTCATTGGGTTTATGCACGGTGACCTGCCGTCACAACTTCAGCCAACTCCGCCTCTCCAGTCCTTCAACTCCTTCTGTAAGTGATCTGCTTTCTGTTGTTGTGTTGTCTTATGTGGAGTTAGGATTCTTGGAATTTTGGCATGTACATTCTTAATTTACCGGCGTTTGTAGAATACAATTGAGTTTCGCAATTTTGGAAATTCTTGTTGCATCCATTTTGGATATAAGACATGTAGCAAATAGTTTTTACATCGAAGCAAGATCATATCAAATTCTCCTCGTTATTGTGTGTACAAACTTGTGCTTATTTTGGATATATTACCCATCTTTAAATTGGTCTCATATCCGACACTTTCTGATGGGTGGCCATCCCTTGATAATTTTCCATGGATTTAGTGAACATGTTAATGGAAGGCTTTCGTTAAAAGTGTAACTGCGGTATAATGCAGCATATTTTTACTAGTGCAGTTGCTAGGTGCAAGAAGTAAAGTAGAACAAGCAAATGAGTTAACTATTTGTCAATGTAATGAACAAGTTCATGACGGAATTACTCTGCTAAGTTGGTTTTATTTTCATATAGGAGATGACTTCCTGCAATACATCATCTAACCCACAGTAGAACCCTTTCCCCAGCAGATTAGGGTAAGCATGTAAAGCAGTTTCTTCATTAGTCTACTGATAAATAAACTTGTTGTTTAAAGTATTTTTTTAGAAGAAAAAAGGTTTTGCTTAAATTAGACAGGTAATATATCCAAAGCGTTTTTTATACTAACATCCAACGTCCTCTTTTAATTTTGTTATTTGTTTAAGAAGCCACAAAAAGAATACAAGAGCAACGAAATTCAAGTTGAATGGAGCCGGCAGGTTTATTCGAATCCTCCATGAGATTTTTCTCTGATCAAGAACTCTGCTATGCTGATATCCTCACTCCTCAAGAGGTATGATGATATTCACTTCATCATACTTATCTTTAATCTTCAATTAGTGTTCCATTATATCTCAAGCATTGATTTTGAATAGGTCGTGGCTAGAATTCAAATAGCCGTCCTCAATTTTCTCAGAATTCTCAACTCCTCCTCTCCAGCCATCTCAAATCTTCCTTTGGTATGCTTCCTTAACTAAGAAATGAACATATATGATAGATGAACTTCAAATTTGGATACAGTTAGACGGTATTAGTGTGTTAATGATTGGACAGATTGATCGAAGATCGAGCAATAGCCGAGTGAGTCAAGGAATTTTGACAGATGATTCGTGGATATTCCTTTCGCATTCCTTTTGTACAAGGTCTTTGATGAGACCCAATGCTTCTAAGGCTTTTGTCAGAGGTATTGTAAATGGAGTCTAATCTTCTGACGGTCAGGTTTTTCGAATGATCACTTTGTTAGCCTCTGTTTTTGTCTACTTTTGAACTTCCCTTATTTTGTGATGTAGTTTGGAAGCTGATGGAGATGTGCTCTCAGATTTTGATTCAGGATAAGCGAGTGACACAGAGGGAGCTTTTCTACAAGCTGCTCTGTGATTCACCGGCTTATTTTTCAACTCAGTTGCATGTCAATAGGACAATTCAAGGTGATTTTGTTCAACGAAAACCAGTTTACTAGTTAAGAACCTAAAACTATATTAAGCATAATGATCAAAGTGGAGTAGTTTCATTGCAGATGTTGTAGCCTTACTTCAATGTAGCCGTTACAGCCTTGGAATTATGGCATCTAGTAGGGGACTTGTCGCGGGCCGTCTACTGTTGCAGGCACATTGCGCTGCTTTTCTTATCGGTTTGGTTATTTACGTTGCACATCACATATATACGCATGAAGTGATAACCGGCTTGTATTGACCCCTATTATTCTACTTTTGCATCACAACTACGTTGTGAATCCTTGAAATCAGGGTATTGATTTGATTATACATTGTTTTTTTGTTTCTGGCCAACATGCACTTTTAAGGTATCTTCTACTAAACCAAATTCTTGGACGTTATGGCCTTCCTGAACAATTTTGATTCCTATAGGAGCCTGAGCAAGAAGTTGTGGACTGCACTGCCTGTGGGTCTTCTGGATATCCCATTTCAGGTGACTTGGACTTGCTGAAGATGTTGACTCTGAAGACCGACGCTCGTTACATAATTGTAATTGAAAAGGTAACTTTTCTTAAATTATGACCTGCTTAATTATACTTTTGTGAAAACAATATTTCAAAAAATGCAGTCTATCATAATGTGTGCAAATGCATCAAACATTATTGGTTATGCAGCATGCGATATTCCAACGATTGGCAGATGATCGCGTTTTCAATCACATTCCTAGTATTCTTATCACAGCTAAAGGATATCCAGATTTAGCCACAAGGTAAGTTTGTTCAACAGTTGTATAAATTGTCATTCCTTGTTGCCTTCTTGGAAGTCGTAACTTAAATATATGTATGAACTGCCTTCTCAGGTACTTTCTTCACAAAATAGGCAAAACCTTTCCGCATCTGCCAATGTTCGGCCTTGTTGATTGGTATGAGATCCTCCTTTACATCAACTCAACAAACTTTAGAATTTTCTTCTCACTTCCAGCTTCTGGGTTCTAAAGGAACCCTGCGGGATTGGCCATTTTATGCACCTTCAAATACGGAAGTATAGGGATGGGCTTGGAGGCATACAGATATGGTAAGTCTCATTCGTTCATTTGTTCGATCTATTTCTAATTGCAAATCATAAAAACTGAAGAATCTCTTTAAATTACCAGCTTGCAATGTTAAATGGTTGGGAGTACGAGGAGATGATTTACAACTGATACCACAAGAATCTCTGGTTCCACTCAAGCCCCGGGACCTACAAATTGCCAAAAGCTTGATGTCCTCAAAAATATTACAGGTATCGTGCATAATTTTATACCTTTGATGCTATGGCATTTTAGGCTATCAAAATGCTTAAGAATATATCAAAACCAACAGGAGAACTATCGACAAGAGCTGACTTTAATGGTTGAGAGGGGGCAAAAGGCTGAACTTGAAGCTTTGTACCACAATGGATTCGATTATTTGGAGAAGTACATAGTCAAAAAGATTATTCAATTTAGTTACATCTGACATTATTTTCAACCTAGAGAAGTAAAGACATCTGCATAGTGTGAATAAAGGGCTACTTGCTAAAAAAACCTTACCTTCTTTTTCAAGTAAGCAAGTCCATCTTTCTTAGAAGAGTACTTCACAAAAACTGTAAGTGGCTCGTTCATCAATCAAAGTTTTCAGCTGCTTCAACTTTAAAGATTCCCCAGGCTCCTTTCCCCACGTTGCCATTCTACGACGTCATCTGAGTCTCCATGACATCAAAGGAAGTCTTTTTCCCTTGAAAGCTACTCTTAATACCCAACCCTTGCTTTCTCAGTTGTAGATTTATCCTATAAAGTTCCAAAGTAGAAAAACTATTAGGTGCCATCGTCTGTGAAAGAAATGTATGACCCATGAACAGAAGATTATCGAACCATTGAACTTCAATATTATTCACTATATCTCCCAAACATTTTGTTGAACAGGTTTGAATGTTGTAAATATATTTATATTTTTCTTGCTTTTAATCTTTT

mRNA sequence

TGGTGATCTCTAACGTGACGAAGGGTAAGCGCGGGATGCAACGGAATCGAAGGTCATCCGTAAGAAACCCGAGGTATAAATTAAAGGGGAGAGCCTCGCCTAGCCCAATGCTTTTTGCAACACTGTCCCATTTTTGGTTTGGAGGGAGTTGTGGACTTGATCATGAAATTGTATCATCAACGTACTCGCAAAAGAAAATGATCTTTACAATGGAACAATCTATAGGCGCTTTGATTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTTCTCTCTCTAGATTTCGAACAACCAACATATAATCAGCGGCATTTTCATTGGGTTTATGCACGGTGACCTGCCGTCACAACTTCAGCCAACTCCGCCTCTCCAGTCCTTCAACTCCTTCTGAGATGACTTCCTGCAATACATCATCTAACCCACAGTAGAACCCTTTCCCCAGCAGATTAGGCCACAAAAAGAATACAAGAGCAACGAAATTCAAGTTGAATGGAGCCGGCAGGTTTATTCGAATCCTCCATGAGATTTTTCTCTGATCAAGAACTCTGCTATGCTGATATCCTCACTCCTCAAGAGGTCGTGGCTAGAATTCAAATAGCCGTCCTCAATTTTCTCAGAATTCTCAACTCCTCCTCTCCAGCCATCTCAAATCTTCCTTTGATTGATCGAAGATCGAGCAATAGCCGAGTGAGTCAAGGAATTTTGACAGATGATTCGTGGATATTCCTTTCGCATTCCTTTTGTACAAGGTCTTTGATGAGACCCAATGCTTCTAAGGCTTTTGTCAGAGTTTGGAAGCTGATGGAGATGTGCTCTCAGATTTTGATTCAGGATAAGCGAGTGACACAGAGGGAGCTTTTCTACAAGCTGCTCTGTGATTCACCGGCTTATTTTTCAACTCAGTTGCATGTCAATAGGACAATTCAAGATGTTGTAGCCTTACTTCAATGTAGCCGTTACAGCCTTGGAATTATGGCATCTAGTAGGGGACTTGTCGCGGGCCGTCTACTGTTGCAGGAGCCTGAGCAAGAAGTTGTGGACTGCACTGCCTGTGGGTCTTCTGGATATCCCATTTCAGGTGACTTGGACTTGCTGAAGATGTTGACTCTGAAGACCGACGCTCGTTACATAATTGTAATTGAAAAGCATGCGATATTCCAACGATTGGCAGATGATCGCGTTTTCAATCACATTCCTAGTATTCTTATCACAGCTAAAGGATATCCAGATTTAGCCACAAGGTACTTTCTTCACAAAATAGGCAAAACCTTTCCGCATCTGCCAATGTTCGGCCTTGTTGATTGGAACCCTGCGGGATTGGCCATTTTATGCACCTTCAAATACGGAAGTATAGGGATGGGCTTGGAGGCATACAGATATGCTTGCAATGTTAAATGGTTGGGAGTACGAGGAGATGATTTACAACTGATACCACAAGAATCTCTGGTTCCACTCAAGCCCCGGGACCTACAAATTGCCAAAAGCTTGATGTCCTCAAAAATATTACAGGAGAACTATCGACAAGAGCTGACTTTAATGGTTGAGAGGGGGCAAAAGGCTGAACTTGAAGCTTTGTACCACAATGGATTCGATTATTTGGAGAAGTACATAGTCAAAAAGATTATTCAATTTAGTTACATCTGACATTATTTTCAACCTAGAGAAGTAAAGACATCTGCATAGTGTGAATAAAGGGCTACTTGCTAAAAAAACCTTACCTTCTTTTTCAAGTAAGCAAGTCCATCTTTCTTAGAAGAGTACTTCACAAAAACTGTAAGTGGCTCGTTCATCAATCAAAGTTTTCAGCTGCTTCAACTTTAAAGATTCCCCAGGCTCCTTTCCCCACGTTGCCATTCTACGACGTCATCTGAGTCTCCATGACATCAAAGGAAGTCTTTTTCCCTTGAAAGCTACTCTTAATACCCAACCCTTGCTTTCTCAGTTGTAGATTTATCCTATAAAGTTCCAAAGTAGAAAAACTATTAGGTGCCATCGTCTGTGAAAGAAATGTATGACCCATGAACAGAAGATTATCGAACCATTGAACTTCAATATTATTCACTATATCTCCCAAACATTTTGTTGAACAGGTTTGAATGTTGTAAATATATTTATATTTTTCTTGCTTTTAATCTTTT

Coding sequence (CDS)

ATGGAGCCGGCAGGTTTATTCGAATCCTCCATGAGATTTTTCTCTGATCAAGAACTCTGCTATGCTGATATCCTCACTCCTCAAGAGGTCGTGGCTAGAATTCAAATAGCCGTCCTCAATTTTCTCAGAATTCTCAACTCCTCCTCTCCAGCCATCTCAAATCTTCCTTTGATTGATCGAAGATCGAGCAATAGCCGAGTGAGTCAAGGAATTTTGACAGATGATTCGTGGATATTCCTTTCGCATTCCTTTTGTACAAGGTCTTTGATGAGACCCAATGCTTCTAAGGCTTTTGTCAGAGTTTGGAAGCTGATGGAGATGTGCTCTCAGATTTTGATTCAGGATAAGCGAGTGACACAGAGGGAGCTTTTCTACAAGCTGCTCTGTGATTCACCGGCTTATTTTTCAACTCAGTTGCATGTCAATAGGACAATTCAAGATGTTGTAGCCTTACTTCAATGTAGCCGTTACAGCCTTGGAATTATGGCATCTAGTAGGGGACTTGTCGCGGGCCGTCTACTGTTGCAGGAGCCTGAGCAAGAAGTTGTGGACTGCACTGCCTGTGGGTCTTCTGGATATCCCATTTCAGGTGACTTGGACTTGCTGAAGATGTTGACTCTGAAGACCGACGCTCGTTACATAATTGTAATTGAAAAGCATGCGATATTCCAACGATTGGCAGATGATCGCGTTTTCAATCACATTCCTAGTATTCTTATCACAGCTAAAGGATATCCAGATTTAGCCACAAGGTACTTTCTTCACAAAATAGGCAAAACCTTTCCGCATCTGCCAATGTTCGGCCTTGTTGATTGGAACCCTGCGGGATTGGCCATTTTATGCACCTTCAAATACGGAAGTATAGGGATGGGCTTGGAGGCATACAGATATGCTTGCAATGTTAAATGGTTGGGAGTACGAGGAGATGATTTACAACTGATACCACAAGAATCTCTGGTTCCACTCAAGCCCCGGGACCTACAAATTGCCAAAAGCTTGATGTCCTCAAAAATATTACAGGAGAACTATCGACAAGAGCTGACTTTAATGGTTGAGAGGGGGCAAAAGGCTGAACTTGAAGCTTTGTACCACAATGGATTCGATTATTTGGAGAAGTACATAGTCAAAAAGATTATTCAATTTAGTTACATCTGA

Protein sequence

MEPAGLFESSMRFFSDQELCYADILTPQEVVARIQIAVLNFLRILNSSSPAISNLPLIDRRSSNSRVSQGILTDDSWIFLSHSFCTRSLMRPNASKAFVRVWKLMEMCSQILIQDKRVTQRELFYKLLCDSPAYFSTQLHVNRTIQDVVALLQCSRYSLGIMASSRGLVAGRLLLQEPEQEVVDCTACGSSGYPISGDLDLLKMLTLKTDARYIIVIEKHAIFQRLADDRVFNHIPSILITAKGYPDLATRYFLHKIGKTFPHLPMFGLVDWNPAGLAILCTFKYGSIGMGLEAYRYACNVKWLGVRGDDLQLIPQESLVPLKPRDLQIAKSLMSSKILQENYRQELTLMVERGQKAELEALYHNGFDYLEKYIVKKIIQFSYI
Homology
BLAST of Cmc09g0258291 vs. NCBI nr
Match: XP_008437337.1 (PREDICTED: meiotic recombination protein SPO11-2 [Cucumis melo] >XP_008437338.1 PREDICTED: meiotic recombination protein SPO11-2 [Cucumis melo] >XP_008437341.1 PREDICTED: meiotic recombination protein SPO11-2 [Cucumis melo] >XP_008437342.1 PREDICTED: meiotic recombination protein SPO11-2 [Cucumis melo])

HSP 1 Score: 765.8 bits (1976), Expect = 1.8e-217
Identity = 384/384 (100.00%), Postives = 384/384 (100.00%), Query Frame = 0

Query: 1   MEPAGLFESSMRFFSDQELCYADILTPQEVVARIQIAVLNFLRILNSSSPAISNLPLIDR 60
           MEPAGLFESSMRFFSDQELCYADILTPQEVVARIQIAVLNFLRILNSSSPAISNLPLIDR
Sbjct: 1   MEPAGLFESSMRFFSDQELCYADILTPQEVVARIQIAVLNFLRILNSSSPAISNLPLIDR 60

Query: 61  RSSNSRVSQGILTDDSWIFLSHSFCTRSLMRPNASKAFVRVWKLMEMCSQILIQDKRVTQ 120
           RSSNSRVSQGILTDDSWIFLSHSFCTRSLMRPNASKAFVRVWKLMEMCSQILIQDKRVTQ
Sbjct: 61  RSSNSRVSQGILTDDSWIFLSHSFCTRSLMRPNASKAFVRVWKLMEMCSQILIQDKRVTQ 120

Query: 121 RELFYKLLCDSPAYFSTQLHVNRTIQDVVALLQCSRYSLGIMASSRGLVAGRLLLQEPEQ 180
           RELFYKLLCDSPAYFSTQLHVNRTIQDVVALLQCSRYSLGIMASSRGLVAGRLLLQEPEQ
Sbjct: 121 RELFYKLLCDSPAYFSTQLHVNRTIQDVVALLQCSRYSLGIMASSRGLVAGRLLLQEPEQ 180

Query: 181 EVVDCTACGSSGYPISGDLDLLKMLTLKTDARYIIVIEKHAIFQRLADDRVFNHIPSILI 240
           EVVDCTACGSSGYPISGDLDLLKMLTLKTDARYIIVIEKHAIFQRLADDRVFNHIPSILI
Sbjct: 181 EVVDCTACGSSGYPISGDLDLLKMLTLKTDARYIIVIEKHAIFQRLADDRVFNHIPSILI 240

Query: 241 TAKGYPDLATRYFLHKIGKTFPHLPMFGLVDWNPAGLAILCTFKYGSIGMGLEAYRYACN 300
           TAKGYPDLATRYFLHKIGKTFPHLPMFGLVDWNPAGLAILCTFKYGSIGMGLEAYRYACN
Sbjct: 241 TAKGYPDLATRYFLHKIGKTFPHLPMFGLVDWNPAGLAILCTFKYGSIGMGLEAYRYACN 300

Query: 301 VKWLGVRGDDLQLIPQESLVPLKPRDLQIAKSLMSSKILQENYRQELTLMVERGQKAELE 360
           VKWLGVRGDDLQLIPQESLVPLKPRDLQIAKSLMSSKILQENYRQELTLMVERGQKAELE
Sbjct: 301 VKWLGVRGDDLQLIPQESLVPLKPRDLQIAKSLMSSKILQENYRQELTLMVERGQKAELE 360

Query: 361 ALYHNGFDYLEKYIVKKIIQFSYI 385
           ALYHNGFDYLEKYIVKKIIQFSYI
Sbjct: 361 ALYHNGFDYLEKYIVKKIIQFSYI 384

BLAST of Cmc09g0258291 vs. NCBI nr
Match: KAA0042721.1 (meiotic recombination protein SPO11-2 [Cucumis melo var. makuwa])

HSP 1 Score: 756.9 bits (1953), Expect = 8.4e-215
Identity = 383/394 (97.21%), Postives = 384/394 (97.46%), Query Frame = 0

Query: 1   MEPAGLFESSMRFFSDQELCYADILTPQEVVARIQIAVLNFLRILNSSSPAISNLPLIDR 60
           MEPAGLFESSMRFFSDQELCYADILTPQEVVARIQIAVLNFLRILNSSSPAISNLPLIDR
Sbjct: 1   MEPAGLFESSMRFFSDQELCYADILTPQEVVARIQIAVLNFLRILNSSSPAISNLPLIDR 60

Query: 61  RSSNSRVSQGILTDDSWIFLSHSFCTRSLMRPNASKAFVRVWKLMEMCSQILIQDKRVTQ 120
           RSSNSRVSQGILTDDSWIFLSHSFCTRSLMRPNASKAFVRVWKLMEMCSQILIQDKRVTQ
Sbjct: 61  RSSNSRVSQGILTDDSWIFLSHSFCTRSLMRPNASKAFVRVWKLMEMCSQILIQDKRVTQ 120

Query: 121 RELFYKLLCDSPAYFSTQLHVNRTIQDVVALLQCSRYSLGIMASSRGLVAGRLLLQ---- 180
           RELFYKLLCDSPAYFSTQLHVNRTIQDVVALLQCSRYSLGIMASSRGLVAGRLLLQ    
Sbjct: 121 RELFYKLLCDSPAYFSTQLHVNRTIQDVVALLQCSRYSLGIMASSRGLVAGRLLLQAHCT 180

Query: 181 ------EPEQEVVDCTACGSSGYPISGDLDLLKMLTLKTDARYIIVIEKHAIFQRLADDR 240
                 EPEQEVVDCTACGSSGYPISGDLDLLKMLTLKTDARYIIVIEKHAIFQRLADDR
Sbjct: 181 AFLIGLEPEQEVVDCTACGSSGYPISGDLDLLKMLTLKTDARYIIVIEKHAIFQRLADDR 240

Query: 241 VFNHIPSILITAKGYPDLATRYFLHKIGKTFPHLPMFGLVDWNPAGLAILCTFKYGSIGM 300
           VFNHIPSILITAKGYPDLATRYFLHKIGKTFPHLPMFGLVDWNPAG+AILCTFKYGSIGM
Sbjct: 241 VFNHIPSILITAKGYPDLATRYFLHKIGKTFPHLPMFGLVDWNPAGMAILCTFKYGSIGM 300

Query: 301 GLEAYRYACNVKWLGVRGDDLQLIPQESLVPLKPRDLQIAKSLMSSKILQENYRQELTLM 360
           GLEAYRYACNVKWLGVRGDDLQLIPQESLVPLKPRDLQIAKSLMSSKILQENYRQELTLM
Sbjct: 301 GLEAYRYACNVKWLGVRGDDLQLIPQESLVPLKPRDLQIAKSLMSSKILQENYRQELTLM 360

Query: 361 VERGQKAELEALYHNGFDYLEKYIVKKIIQFSYI 385
           VERGQKAELEALYHNGFDYLEKYIVKKIIQFSYI
Sbjct: 361 VERGQKAELEALYHNGFDYLEKYIVKKIIQFSYI 394

BLAST of Cmc09g0258291 vs. NCBI nr
Match: XP_004143970.1 (meiotic recombination protein SPO11-2 [Cucumis sativus] >XP_011654717.1 meiotic recombination protein SPO11-2 [Cucumis sativus] >XP_011654718.1 meiotic recombination protein SPO11-2 [Cucumis sativus] >XP_031741008.1 meiotic recombination protein SPO11-2 [Cucumis sativus] >KGN50057.1 hypothetical protein Csa_000678 [Cucumis sativus])

HSP 1 Score: 748.0 bits (1930), Expect = 3.9e-212
Identity = 375/384 (97.66%), Postives = 379/384 (98.70%), Query Frame = 0

Query: 1   MEPAGLFESSMRFFSDQELCYADILTPQEVVARIQIAVLNFLRILNSSSPAISNLPLIDR 60
           MEPAGLFESSMRFFSDQELCYADILTPQEVVARIQ+AVLNFLRILNSSSPAISNLPLIDR
Sbjct: 1   MEPAGLFESSMRFFSDQELCYADILTPQEVVARIQVAVLNFLRILNSSSPAISNLPLIDR 60

Query: 61  RSSNSRVSQGILTDDSWIFLSHSFCTRSLMRPNASKAFVRVWKLMEMCSQILIQDKRVTQ 120
           RSSNSRVSQGILTDDSWIFLSHSFCTRSLMRPNASKAFVRVWKLMEMCSQILIQDK+VTQ
Sbjct: 61  RSSNSRVSQGILTDDSWIFLSHSFCTRSLMRPNASKAFVRVWKLMEMCSQILIQDKKVTQ 120

Query: 121 RELFYKLLCDSPAYFSTQLHVNRTIQDVVALLQCSRYSLGIMASSRGLVAGRLLLQEPEQ 180
           RELFYKLLCDSP YFSTQLHVNRTIQDVVALLQCSRYSLGIMASSRGLVAGRLLLQEPEQ
Sbjct: 121 RELFYKLLCDSPVYFSTQLHVNRTIQDVVALLQCSRYSLGIMASSRGLVAGRLLLQEPEQ 180

Query: 181 EVVDCTACGSSGYPISGDLDLLKMLTLKTDARYIIVIEKHAIFQRLADDRVFNHIPSILI 240
           EVVDCTACGSSGYPISGDLDLL+ LTLKTDARYIIVIEKHAIFQRLADDRVFN IPSILI
Sbjct: 181 EVVDCTACGSSGYPISGDLDLLQTLTLKTDARYIIVIEKHAIFQRLADDRVFNRIPSILI 240

Query: 241 TAKGYPDLATRYFLHKIGKTFPHLPMFGLVDWNPAGLAILCTFKYGSIGMGLEAYRYACN 300
           TAKGYPDLATRYFLHKI KTF HLPMFGLVDWNPAGLAILCTFKYGSIGMGLEAYRYACN
Sbjct: 241 TAKGYPDLATRYFLHKICKTFSHLPMFGLVDWNPAGLAILCTFKYGSIGMGLEAYRYACN 300

Query: 301 VKWLGVRGDDLQLIPQESLVPLKPRDLQIAKSLMSSKILQENYRQELTLMVERGQKAELE 360
           VKWLGVRGDDLQLIPQESLVPLKPRDLQIAKSL+SSKILQENYRQELTLMVERGQKAELE
Sbjct: 301 VKWLGVRGDDLQLIPQESLVPLKPRDLQIAKSLLSSKILQENYRQELTLMVERGQKAELE 360

Query: 361 ALYHNGFDYLEKYIVKKIIQFSYI 385
           ALYHNGFDYLEKYIVKKIIQFSYI
Sbjct: 361 ALYHNGFDYLEKYIVKKIIQFSYI 384

BLAST of Cmc09g0258291 vs. NCBI nr
Match: XP_038906472.1 (meiotic recombination protein SPO11-2 isoform X1 [Benincasa hispida] >XP_038906473.1 meiotic recombination protein SPO11-2 isoform X1 [Benincasa hispida] >XP_038906474.1 meiotic recombination protein SPO11-2 isoform X1 [Benincasa hispida] >XP_038906475.1 meiotic recombination protein SPO11-2 isoform X1 [Benincasa hispida] >XP_038906476.1 meiotic recombination protein SPO11-2 isoform X1 [Benincasa hispida])

HSP 1 Score: 725.3 bits (1871), Expect = 2.7e-205
Identity = 363/384 (94.53%), Postives = 373/384 (97.14%), Query Frame = 0

Query: 1   MEPAGLFESSMRFFSDQELCYADILTPQEVVARIQIAVLNFLRILNSSSPAISNLPLIDR 60
           MEP GLF+SSMRFFSDQELCYADILTPQEVVARIQIAVLNFLRIL SS+PAISNLPLIDR
Sbjct: 1   MEPTGLFKSSMRFFSDQELCYADILTPQEVVARIQIAVLNFLRILTSSTPAISNLPLIDR 60

Query: 61  RSSNSRVSQGILTDDSWIFLSHSFCTRSLMRPNASKAFVRVWKLMEMCSQILIQDKRVTQ 120
           RSSNSRV+QGILTDD WIFLSHSFCTRSLMR NA+KAFVRVWKLMEMCSQILIQDKRVTQ
Sbjct: 61  RSSNSRVNQGILTDDLWIFLSHSFCTRSLMRSNAAKAFVRVWKLMEMCSQILIQDKRVTQ 120

Query: 121 RELFYKLLCDSPAYFSTQLHVNRTIQDVVALLQCSRYSLGIMASSRGLVAGRLLLQEPEQ 180
           RELFYKLLCDSPAYF TQLHVNRTIQDVVALLQCSR SLGIMASSRGLVAGRLLLQEPEQ
Sbjct: 121 RELFYKLLCDSPAYFPTQLHVNRTIQDVVALLQCSRCSLGIMASSRGLVAGRLLLQEPEQ 180

Query: 181 EVVDCTACGSSGYPISGDLDLLKMLTLKTDARYIIVIEKHAIFQRLADDRVFNHIPSILI 240
           EVVDCTACGSSGY ISGDLDLL+ LTLKTDARYIIVIEKHAIFQRLA+DR+FNHIPSILI
Sbjct: 181 EVVDCTACGSSGYAISGDLDLLQKLTLKTDARYIIVIEKHAIFQRLAEDRIFNHIPSILI 240

Query: 241 TAKGYPDLATRYFLHKIGKTFPHLPMFGLVDWNPAGLAILCTFKYGSIGMGLEAYRYACN 300
           TAKGYPDLATR+FLH+I KTFPHLP+FGLVDWNPAGLAILCTFKYGSIGMGLEAYRYACN
Sbjct: 241 TAKGYPDLATRFFLHRISKTFPHLPIFGLVDWNPAGLAILCTFKYGSIGMGLEAYRYACN 300

Query: 301 VKWLGVRGDDLQLIPQESLVPLKPRDLQIAKSLMSSKILQENYRQELTLMVERGQKAELE 360
           VKWLGVRGDDLQLIPQESLVPLKPRDLQIAKSLMSSKILQE YRQELTLMVE GQKAELE
Sbjct: 301 VKWLGVRGDDLQLIPQESLVPLKPRDLQIAKSLMSSKILQEKYRQELTLMVEIGQKAELE 360

Query: 361 ALYHNGFDYLEKYIVKKIIQFSYI 385
           ALYHNGFDYLEKYIVKKIIQFSYI
Sbjct: 361 ALYHNGFDYLEKYIVKKIIQFSYI 384

BLAST of Cmc09g0258291 vs. NCBI nr
Match: XP_023533741.1 (meiotic recombination protein SPO11-2 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 707.2 bits (1824), Expect = 7.7e-200
Identity = 347/384 (90.36%), Postives = 372/384 (96.88%), Query Frame = 0

Query: 1   MEPAGLFESSMRFFSDQELCYADILTPQEVVARIQIAVLNFLRILNSSSPAISNLPLIDR 60
           ME  GLF+SSMRFFSDQELCYADILTPQEV+ARIQ+AVLNFLRIL+SS+PAISNLPLIDR
Sbjct: 1   METTGLFKSSMRFFSDQELCYADILTPQEVLARIQVAVLNFLRILSSSTPAISNLPLIDR 60

Query: 61  RSSNSRVSQGILTDDSWIFLSHSFCTRSLMRPNASKAFVRVWKLMEMCSQILIQDKRVTQ 120
            SSNSRV+QGILTD+SWIFLSHSFCTRSLMR NA+KAFVRVWKLMEMCSQIL+Q+KRVTQ
Sbjct: 61  SSSNSRVNQGILTDESWIFLSHSFCTRSLMRANAAKAFVRVWKLMEMCSQILLQEKRVTQ 120

Query: 121 RELFYKLLCDSPAYFSTQLHVNRTIQDVVALLQCSRYSLGIMASSRGLVAGRLLLQEPEQ 180
           RELFYKLLCDSPAYF TQLHVNRTIQDVVALL+CSR+SLG+MASSRGLVAGRLLLQEPEQ
Sbjct: 121 RELFYKLLCDSPAYFPTQLHVNRTIQDVVALLRCSRFSLGVMASSRGLVAGRLLLQEPEQ 180

Query: 181 EVVDCTACGSSGYPISGDLDLLKMLTLKTDARYIIVIEKHAIFQRLADDRVFNHIPSILI 240
           E VDC ACGSSGY ISGDLDLL+ LTLKTDARY+IVIEKHAIFQRLA+DRVFNHIPSILI
Sbjct: 181 ETVDCAACGSSGYAISGDLDLLQKLTLKTDARYVIVIEKHAIFQRLAEDRVFNHIPSILI 240

Query: 241 TAKGYPDLATRYFLHKIGKTFPHLPMFGLVDWNPAGLAILCTFKYGSIGMGLEAYRYACN 300
           TAKGYPD+ATRYFLH+I KTFPHLP+FGLVDWNPAGLAILCTFKYGSIGMGLEAYRYACN
Sbjct: 241 TAKGYPDIATRYFLHRISKTFPHLPIFGLVDWNPAGLAILCTFKYGSIGMGLEAYRYACN 300

Query: 301 VKWLGVRGDDLQLIPQESLVPLKPRDLQIAKSLMSSKILQENYRQELTLMVERGQKAELE 360
           VKWLG+RGDD+QLIPQESLV LKPRDLQIAKSLMSS+ILQENYR+ELTLMVE GQKAELE
Sbjct: 301 VKWLGMRGDDVQLIPQESLVSLKPRDLQIAKSLMSSEILQENYREELTLMVEGGQKAELE 360

Query: 361 ALYHNGFDYLEKYIVKKIIQFSYI 385
           ALYHNGFDYLEKY+VKKIIQF+YI
Sbjct: 361 ALYHNGFDYLEKYLVKKIIQFNYI 384

BLAST of Cmc09g0258291 vs. ExPASy Swiss-Prot
Match: Q9M4A1 (Meiotic recombination protein SPO11-2 OS=Arabidopsis thaliana OX=3702 GN=SPO11-2 PE=1 SV=1)

HSP 1 Score: 557.8 bits (1436), Expect = 9.8e-158
Identity = 274/383 (71.54%), Postives = 328/383 (85.64%), Query Frame = 0

Query: 2   EPAGLFESSMRFFSDQELCYADILTPQEVVARIQIAVLNFLRILNSSSPAISNLPLIDRR 61
           E +GL  SSM+FFSDQ L YADIL P E  ARI+++VLN LRILNS  PAIS+L LI+R+
Sbjct: 3   ESSGL--SSMKFFSDQHLSYADILLPHEARARIEVSVLNLLRILNSPDPAISDLSLINRK 62

Query: 62  SSNSRVSQGILTDDSWIFLSHSFCTRSLMRPNASKAFVRVWKLMEMCSQILIQDKRVTQR 121
            SNS +++GILTD S+IFLS SF   SL     +KAFVRVWK+ME+C QIL+Q+KRVTQR
Sbjct: 63  RSNSCINKGILTDVSYIFLSTSFTKSSLTNAKTAKAFVRVWKVMEICFQILLQEKRVTQR 122

Query: 122 ELFYKLLCDSPAYFSTQLHVNRTIQDVVALLQCSRYSLGIMASSRGLVAGRLLLQEPEQE 181
           ELFYKLLCDSP YFS+Q+ VNR++QDVVALL+CSRYSLGIMASSRGLVAGRL LQEP +E
Sbjct: 123 ELFYKLLCDSPDYFSSQIEVNRSVQDVVALLRCSRYSLGIMASSRGLVAGRLFLQEPGKE 182

Query: 182 VVDCTACGSSGYPISGDLDLLKMLTLKTDARYIIVIEKHAIFQRLADDRVFNHIPSILIT 241
            VDC+ACGSSG+ I+GDL+LL    ++TDARYII++EKHAIF RL +DRVFNHIP + IT
Sbjct: 183 AVDCSACGSSGFAITGDLNLLDNTIMRTDARYIIIVEKHAIFHRLVEDRVFNHIPCVFIT 242

Query: 242 AKGYPDLATRYFLHKIGKTFPHLPMFGLVDWNPAGLAILCTFKYGSIGMGLEAYRYACNV 301
           AKGYPD+ATR+FLH++  TFP LP+  LVDWNPAGLAILCTFK+GSIGMGLEAYRYACNV
Sbjct: 243 AKGYPDIATRFFLHRMSTTFPDLPILVLVDWNPAGLAILCTFKFGSIGMGLEAYRYACNV 302

Query: 302 KWLGVRGDDLQLIPQESLVPLKPRDLQIAKSLMSSKILQENYRQELTLMVERGQKAELEA 361
           KW+G+RGDDL LIP+ESLVPLKP+D QIAKSL+SSKILQENY +EL+LMV+ G++AE+EA
Sbjct: 303 KWIGLRGDDLNLIPEESLVPLKPKDSQIAKSLLSSKILQENYIEELSLMVQTGKRAEIEA 362

Query: 362 LYHNGFDYLEKYIVKKIIQFSYI 385
           LY +G++YL KYI  KI+Q  YI
Sbjct: 363 LYCHGYNYLGKYIATKIVQGKYI 383

BLAST of Cmc09g0258291 vs. ExPASy Swiss-Prot
Match: Q5ZPI9 (Meiotic recombination protein SPO11-2 OS=Oryza sativa subsp. indica OX=39946 GN=SPO11-2 PE=1 SV=1)

HSP 1 Score: 490.0 bits (1260), Expect = 2.5e-137
Identity = 244/386 (63.21%), Postives = 313/386 (81.09%), Query Frame = 0

Query: 1   MEPAGLFESSMRFFSDQELCYADILTPQEVVARIQIAVLNFLRIL-NSSSPAISNLPLID 60
           M  AG+  +S+ F +D+ LC ADIL P EV ARI++AVLNFL  L + ++PAIS LPLI 
Sbjct: 1   MAEAGVAAASL-FGADRRLCSADILPPAEVRARIEVAVLNFLAALTDPAAPAISALPLIS 60

Query: 61  RRSSNSRVSQGILTDD-SWIFLSHSFCTRSLMRPNASKAFVRVWKLMEMCSQILIQDKRV 120
           R ++N  + + +L DD S ++LS++ C RSL R N +KAFVRVWK+MEMC +IL + K V
Sbjct: 61  RGAANRGLRRALLRDDVSSVYLSYASCKRSLTRANDAKAFVRVWKVMEMCYKILGEGKLV 120

Query: 121 TQRELFYKLLCDSPAYFSTQLHVNRTIQDVVALLQCSRYSLGIMASSRGLVAGRLLLQEP 180
           T RELFY LL +SP YF+ Q HVN+T+QDVV+LL+C+R SLGIMASSRG + GRL+LQ P
Sbjct: 121 TLRELFYTLLSESPTYFTCQRHVNQTVQDVVSLLRCTRQSLGIMASSRGALIGRLVLQGP 180

Query: 181 EQEVVDCTACGSSGYPISGDLDLLKMLTLKTDARYIIVIEKHAIFQRLADDRVFNHIPSI 240
           E+E VDC+  G SG+ I+GDL++L  L   +DARYIIV+EK AIFQRLA+DR+++H+P I
Sbjct: 181 EEEHVDCSILGPSGHAITGDLNVLSKLIFSSDARYIIVVEKDAIFQRLAEDRIYSHLPCI 240

Query: 241 LITAKGYPDLATRYFLHKIGKTFPHLPMFGLVDWNPAGLAILCTFKYGSIGMGLEAYRYA 300
           LITAKGYPDLATR+ LH++ +T+P++P+F LVDWNPAGLAILCT+KYGSI MGLE+YRYA
Sbjct: 241 LITAKGYPDLATRFILHRLSQTYPNMPIFALVDWNPAGLAILCTYKYGSISMGLESYRYA 300

Query: 301 CNVKWLGVRGDDLQLIPQESLVPLKPRDLQIAKSLMSSKILQENYRQELTLMVERGQKAE 360
           CNVKWLG+RGDDLQLIPQ +   LKPRDLQIAKSL+SSK LQ+ +R ELTLM+E G++AE
Sbjct: 301 CNVKWLGLRGDDLQLIPQSAYQELKPRDLQIAKSLLSSKFLQDKHRAELTLMLETGKRAE 360

Query: 361 LEALYHNGFDYLEKYIVKKIIQFSYI 385
           +EALY +GFD+L KY+ +KI+Q  YI
Sbjct: 361 IEALYSHGFDFLGKYVARKIVQGDYI 385

BLAST of Cmc09g0258291 vs. ExPASy Swiss-Prot
Match: Q6ZD95 (Meiotic recombination protein SPO11-2 OS=Oryza sativa subsp. japonica OX=39947 GN=SPO11-2 PE=2 SV=1)

HSP 1 Score: 488.8 bits (1257), Expect = 5.6e-137
Identity = 243/386 (62.95%), Postives = 313/386 (81.09%), Query Frame = 0

Query: 1   MEPAGLFESSMRFFSDQELCYADILTPQEVVARIQIAVLNFLRIL-NSSSPAISNLPLID 60
           M  AG+  +S+ F +D+ LC ADIL P EV ARI++AVLNFL  L + ++PAIS LPLI 
Sbjct: 1   MAEAGVAAASL-FGADRRLCSADILPPAEVRARIEVAVLNFLAALTDPAAPAISALPLIS 60

Query: 61  RRSSNSRVSQGILTDD-SWIFLSHSFCTRSLMRPNASKAFVRVWKLMEMCSQILIQDKRV 120
           R ++N  + + +L DD S ++LS++ C RSL R N +KAFVRVWK+MEMC +IL + K V
Sbjct: 61  RGAANRGLRRALLRDDVSSVYLSYASCKRSLTRANDAKAFVRVWKVMEMCYKILGEGKLV 120

Query: 121 TQRELFYKLLCDSPAYFSTQLHVNRTIQDVVALLQCSRYSLGIMASSRGLVAGRLLLQEP 180
           T RELFY LL +SP YF+ Q HVN+T+QDVV+LL+C+R SLGIMASSRG + GRL++Q P
Sbjct: 121 TLRELFYTLLSESPTYFTCQRHVNQTVQDVVSLLRCTRQSLGIMASSRGALIGRLVVQGP 180

Query: 181 EQEVVDCTACGSSGYPISGDLDLLKMLTLKTDARYIIVIEKHAIFQRLADDRVFNHIPSI 240
           E+E VDC+  G SG+ I+GDL++L  L   +DARYIIV+EK AIFQRLA+DR+++H+P I
Sbjct: 181 EEEHVDCSILGPSGHAITGDLNVLSKLIFSSDARYIIVVEKDAIFQRLAEDRIYSHLPCI 240

Query: 241 LITAKGYPDLATRYFLHKIGKTFPHLPMFGLVDWNPAGLAILCTFKYGSIGMGLEAYRYA 300
           LITAKGYPDLATR+ LH++ +T+P++P+F LVDWNPAGLAILCT+KYGSI MGLE+YRYA
Sbjct: 241 LITAKGYPDLATRFILHRLSQTYPNMPIFALVDWNPAGLAILCTYKYGSISMGLESYRYA 300

Query: 301 CNVKWLGVRGDDLQLIPQESLVPLKPRDLQIAKSLMSSKILQENYRQELTLMVERGQKAE 360
           CNVKWLG+RGDDLQLIPQ +   LKPRDLQIAKSL+SSK LQ+ +R ELTLM+E G++AE
Sbjct: 301 CNVKWLGLRGDDLQLIPQSAYQELKPRDLQIAKSLLSSKFLQDKHRAELTLMLETGKRAE 360

Query: 361 LEALYHNGFDYLEKYIVKKIIQFSYI 385
           +EALY +GFD+L KY+ +KI+Q  YI
Sbjct: 361 IEALYSHGFDFLGKYVARKIVQGDYI 385

BLAST of Cmc09g0258291 vs. ExPASy Swiss-Prot
Match: Q9LZ03 (DNA topoisomerase 6 subunit A OS=Arabidopsis thaliana OX=3702 GN=TOP6A PE=1 SV=1)

HSP 1 Score: 181.8 bits (460), Expect = 1.5e-44
Identity = 108/308 (35.06%), Postives = 182/308 (59.09%), Query Frame = 0

Query: 87  RSLMRPNASKAFVR----VWKLMEMCSQILIQDKRVTQRELFYKLLCDSPAYFSTQLHVN 146
           +S +RP AS + VR      +++ +  Q+ +++  VT+R+LFY         F  Q   +
Sbjct: 128 KSTLRPFASVSSVRKTTITTRILALIHQLCLRNIHVTKRDLFY----TDVKLFQDQTQSD 187

Query: 147 RTIQDVVALLQCSRYSLGIMASSRGLVAGRLLLQEPEQEVVDCTACGSSGYPISGDLDLL 206
             + DV  +L C+R SL ++A+ +G+V GRL+  +   +++DCT  G  G  I  ++D  
Sbjct: 188 AVLDDVSCMLGCTRSSLNVIAAEKGVVVGRLIFSD-NGDMIDCTKMGMGGKAIPPNID-- 247

Query: 207 KMLTLKTDARYIIVIEKHAIFQRLADDRVFNHIPSILITAKGYPDLATRYFLHKIGKTFP 266
           ++  +++DA +I+++EK A + RLA+DR +N  P I++TAKG PD+ATR FL K+ K   
Sbjct: 248 RVGDMQSDAMFILLVEKDAAYMRLAEDRFYNRFPCIIVTAKGQPDVATRLFLRKM-KMEL 307

Query: 267 HLPMFGLVDWNPAGLAILCTFKYGSIGMGLEAYRYAC-NVKWLGVRGDDLQ--LIPQESL 326
            LP+  LVD +P GL IL  +  GS  M  ++      ++KWLG+R  DL    IP++  
Sbjct: 308 KLPVLALVDSDPYGLKILSVYGCGSKNMSYDSANLTTPDIKWLGIRPSDLDKYKIPEQCR 367

Query: 327 VPLKPRDLQIAKSLMSSKILQEN--YRQELTLMVERGQKAELEALYHNGFDYL-EKYIVK 385
           +P+  +D++  K ++    +++N  + +EL LMV+  QKAE++AL   GF YL E Y+  
Sbjct: 368 LPMTEQDIKTGKDMLEEDFVKKNPGWVEELNLMVKTKQKAEIQALSSFGFQYLSEVYLPL 427

BLAST of Cmc09g0258291 vs. ExPASy Swiss-Prot
Match: Q9Y5K1 (Meiotic recombination protein SPO11 OS=Homo sapiens OX=9606 GN=SPO11 PE=1 SV=1)

HSP 1 Score: 181.4 bits (459), Expect = 1.9e-44
Identity = 119/368 (32.34%), Postives = 205/368 (55.71%), Query Frame = 0

Query: 24  ILTPQEVVARIQIAVLNFLRIL-NSSSPAISNLPLIDRRSSNSRVSQGILTDDSWIFLSH 83
           + +  EV+A I+  + + +  L  + +PA +    ID RSS     + I  +DS      
Sbjct: 41  LASSSEVLASIENIIQDIITSLARNEAPAFT----IDNRSS----WENIKFEDSVGLQMV 100

Query: 84  SFCTRSLMR---PNASKAFVRVWKLMEMCSQILIQDKRVTQRELFYKLLCDSPAYFSTQL 143
           S CT   ++   P +++ F  + K++ M  +++  +   T+R+++Y         F  Q 
Sbjct: 101 SHCTTRKIKSDSPKSAQKFSLILKILSMIYKLVQSNTYATKRDIYY----TDSQLFGNQT 160

Query: 144 HVNRTIQDVVALLQCSRYSLGIMASSRGLVAGRLLLQEPEQEVVDCTACGSSGYPISGDL 203
            V+  I D+  +L+ SR SL I+++S+GL+AG L   E +   V+CT CG++   +  ++
Sbjct: 161 VVDNIINDISCMLKVSRRSLHILSTSKGLIAGNLRYIEEDGTKVNCT-CGATAVAVPSNI 220

Query: 204 DLLKMLTLKTDARYIIVIEKHAIFQRLADDRVFNHI-PSILITAKGYPDLATRYFLHKIG 263
             ++   L TDA++++++EK A FQRL DD   N + P I+IT KG PDL TR  + K+ 
Sbjct: 221 QGIR--NLVTDAKFVLIVEKDATFQRLLDDNFCNKLSPCIMITGKGVPDLNTRLLVKKLW 280

Query: 264 KTFPHLPMFGLVDWNPAGLAILCTFKYGSIGMGLEAYRYAC-NVKWLGVRGDDLQL--IP 323
            TF H+P+F LVD +P G+ I+C +KYGS+ M  EA+      ++WLG+   DL+   +P
Sbjct: 281 DTF-HVPVFTLVDADPHGIEIMCIYKYGSMSMSFEAHHLTVPAIRWLGLLPSDLKRLNVP 340

Query: 324 QESLVPLKPRDLQIAKSLMSSKIL--QENYRQELTLMVERGQKAELEALYHNGFDYLEKY 382
           ++SL+PL  RD     S++    +  Q  +R+E+ +M +   KAE++AL     DYL + 
Sbjct: 341 KDSLIPLTKRDQMKLDSILRRPYVTCQPFWRKEMEIMADSKMKAEIQALTFLSSDYLSRV 392

BLAST of Cmc09g0258291 vs. ExPASy TrEMBL
Match: A0A1S3AUC6 (meiotic recombination protein SPO11-2 OS=Cucumis melo OX=3656 GN=LOC103482788 PE=3 SV=1)

HSP 1 Score: 765.8 bits (1976), Expect = 8.8e-218
Identity = 384/384 (100.00%), Postives = 384/384 (100.00%), Query Frame = 0

Query: 1   MEPAGLFESSMRFFSDQELCYADILTPQEVVARIQIAVLNFLRILNSSSPAISNLPLIDR 60
           MEPAGLFESSMRFFSDQELCYADILTPQEVVARIQIAVLNFLRILNSSSPAISNLPLIDR
Sbjct: 1   MEPAGLFESSMRFFSDQELCYADILTPQEVVARIQIAVLNFLRILNSSSPAISNLPLIDR 60

Query: 61  RSSNSRVSQGILTDDSWIFLSHSFCTRSLMRPNASKAFVRVWKLMEMCSQILIQDKRVTQ 120
           RSSNSRVSQGILTDDSWIFLSHSFCTRSLMRPNASKAFVRVWKLMEMCSQILIQDKRVTQ
Sbjct: 61  RSSNSRVSQGILTDDSWIFLSHSFCTRSLMRPNASKAFVRVWKLMEMCSQILIQDKRVTQ 120

Query: 121 RELFYKLLCDSPAYFSTQLHVNRTIQDVVALLQCSRYSLGIMASSRGLVAGRLLLQEPEQ 180
           RELFYKLLCDSPAYFSTQLHVNRTIQDVVALLQCSRYSLGIMASSRGLVAGRLLLQEPEQ
Sbjct: 121 RELFYKLLCDSPAYFSTQLHVNRTIQDVVALLQCSRYSLGIMASSRGLVAGRLLLQEPEQ 180

Query: 181 EVVDCTACGSSGYPISGDLDLLKMLTLKTDARYIIVIEKHAIFQRLADDRVFNHIPSILI 240
           EVVDCTACGSSGYPISGDLDLLKMLTLKTDARYIIVIEKHAIFQRLADDRVFNHIPSILI
Sbjct: 181 EVVDCTACGSSGYPISGDLDLLKMLTLKTDARYIIVIEKHAIFQRLADDRVFNHIPSILI 240

Query: 241 TAKGYPDLATRYFLHKIGKTFPHLPMFGLVDWNPAGLAILCTFKYGSIGMGLEAYRYACN 300
           TAKGYPDLATRYFLHKIGKTFPHLPMFGLVDWNPAGLAILCTFKYGSIGMGLEAYRYACN
Sbjct: 241 TAKGYPDLATRYFLHKIGKTFPHLPMFGLVDWNPAGLAILCTFKYGSIGMGLEAYRYACN 300

Query: 301 VKWLGVRGDDLQLIPQESLVPLKPRDLQIAKSLMSSKILQENYRQELTLMVERGQKAELE 360
           VKWLGVRGDDLQLIPQESLVPLKPRDLQIAKSLMSSKILQENYRQELTLMVERGQKAELE
Sbjct: 301 VKWLGVRGDDLQLIPQESLVPLKPRDLQIAKSLMSSKILQENYRQELTLMVERGQKAELE 360

Query: 361 ALYHNGFDYLEKYIVKKIIQFSYI 385
           ALYHNGFDYLEKYIVKKIIQFSYI
Sbjct: 361 ALYHNGFDYLEKYIVKKIIQFSYI 384

BLAST of Cmc09g0258291 vs. ExPASy TrEMBL
Match: A0A5A7TMD5 (Meiotic recombination protein SPO11-2 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold44G002190 PE=3 SV=1)

HSP 1 Score: 756.9 bits (1953), Expect = 4.1e-215
Identity = 383/394 (97.21%), Postives = 384/394 (97.46%), Query Frame = 0

Query: 1   MEPAGLFESSMRFFSDQELCYADILTPQEVVARIQIAVLNFLRILNSSSPAISNLPLIDR 60
           MEPAGLFESSMRFFSDQELCYADILTPQEVVARIQIAVLNFLRILNSSSPAISNLPLIDR
Sbjct: 1   MEPAGLFESSMRFFSDQELCYADILTPQEVVARIQIAVLNFLRILNSSSPAISNLPLIDR 60

Query: 61  RSSNSRVSQGILTDDSWIFLSHSFCTRSLMRPNASKAFVRVWKLMEMCSQILIQDKRVTQ 120
           RSSNSRVSQGILTDDSWIFLSHSFCTRSLMRPNASKAFVRVWKLMEMCSQILIQDKRVTQ
Sbjct: 61  RSSNSRVSQGILTDDSWIFLSHSFCTRSLMRPNASKAFVRVWKLMEMCSQILIQDKRVTQ 120

Query: 121 RELFYKLLCDSPAYFSTQLHVNRTIQDVVALLQCSRYSLGIMASSRGLVAGRLLLQ---- 180
           RELFYKLLCDSPAYFSTQLHVNRTIQDVVALLQCSRYSLGIMASSRGLVAGRLLLQ    
Sbjct: 121 RELFYKLLCDSPAYFSTQLHVNRTIQDVVALLQCSRYSLGIMASSRGLVAGRLLLQAHCT 180

Query: 181 ------EPEQEVVDCTACGSSGYPISGDLDLLKMLTLKTDARYIIVIEKHAIFQRLADDR 240
                 EPEQEVVDCTACGSSGYPISGDLDLLKMLTLKTDARYIIVIEKHAIFQRLADDR
Sbjct: 181 AFLIGLEPEQEVVDCTACGSSGYPISGDLDLLKMLTLKTDARYIIVIEKHAIFQRLADDR 240

Query: 241 VFNHIPSILITAKGYPDLATRYFLHKIGKTFPHLPMFGLVDWNPAGLAILCTFKYGSIGM 300
           VFNHIPSILITAKGYPDLATRYFLHKIGKTFPHLPMFGLVDWNPAG+AILCTFKYGSIGM
Sbjct: 241 VFNHIPSILITAKGYPDLATRYFLHKIGKTFPHLPMFGLVDWNPAGMAILCTFKYGSIGM 300

Query: 301 GLEAYRYACNVKWLGVRGDDLQLIPQESLVPLKPRDLQIAKSLMSSKILQENYRQELTLM 360
           GLEAYRYACNVKWLGVRGDDLQLIPQESLVPLKPRDLQIAKSLMSSKILQENYRQELTLM
Sbjct: 301 GLEAYRYACNVKWLGVRGDDLQLIPQESLVPLKPRDLQIAKSLMSSKILQENYRQELTLM 360

Query: 361 VERGQKAELEALYHNGFDYLEKYIVKKIIQFSYI 385
           VERGQKAELEALYHNGFDYLEKYIVKKIIQFSYI
Sbjct: 361 VERGQKAELEALYHNGFDYLEKYIVKKIIQFSYI 394

BLAST of Cmc09g0258291 vs. ExPASy TrEMBL
Match: A0A0A0KNU2 (TP6A_N domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G152220 PE=3 SV=1)

HSP 1 Score: 748.0 bits (1930), Expect = 1.9e-212
Identity = 375/384 (97.66%), Postives = 379/384 (98.70%), Query Frame = 0

Query: 1   MEPAGLFESSMRFFSDQELCYADILTPQEVVARIQIAVLNFLRILNSSSPAISNLPLIDR 60
           MEPAGLFESSMRFFSDQELCYADILTPQEVVARIQ+AVLNFLRILNSSSPAISNLPLIDR
Sbjct: 1   MEPAGLFESSMRFFSDQELCYADILTPQEVVARIQVAVLNFLRILNSSSPAISNLPLIDR 60

Query: 61  RSSNSRVSQGILTDDSWIFLSHSFCTRSLMRPNASKAFVRVWKLMEMCSQILIQDKRVTQ 120
           RSSNSRVSQGILTDDSWIFLSHSFCTRSLMRPNASKAFVRVWKLMEMCSQILIQDK+VTQ
Sbjct: 61  RSSNSRVSQGILTDDSWIFLSHSFCTRSLMRPNASKAFVRVWKLMEMCSQILIQDKKVTQ 120

Query: 121 RELFYKLLCDSPAYFSTQLHVNRTIQDVVALLQCSRYSLGIMASSRGLVAGRLLLQEPEQ 180
           RELFYKLLCDSP YFSTQLHVNRTIQDVVALLQCSRYSLGIMASSRGLVAGRLLLQEPEQ
Sbjct: 121 RELFYKLLCDSPVYFSTQLHVNRTIQDVVALLQCSRYSLGIMASSRGLVAGRLLLQEPEQ 180

Query: 181 EVVDCTACGSSGYPISGDLDLLKMLTLKTDARYIIVIEKHAIFQRLADDRVFNHIPSILI 240
           EVVDCTACGSSGYPISGDLDLL+ LTLKTDARYIIVIEKHAIFQRLADDRVFN IPSILI
Sbjct: 181 EVVDCTACGSSGYPISGDLDLLQTLTLKTDARYIIVIEKHAIFQRLADDRVFNRIPSILI 240

Query: 241 TAKGYPDLATRYFLHKIGKTFPHLPMFGLVDWNPAGLAILCTFKYGSIGMGLEAYRYACN 300
           TAKGYPDLATRYFLHKI KTF HLPMFGLVDWNPAGLAILCTFKYGSIGMGLEAYRYACN
Sbjct: 241 TAKGYPDLATRYFLHKICKTFSHLPMFGLVDWNPAGLAILCTFKYGSIGMGLEAYRYACN 300

Query: 301 VKWLGVRGDDLQLIPQESLVPLKPRDLQIAKSLMSSKILQENYRQELTLMVERGQKAELE 360
           VKWLGVRGDDLQLIPQESLVPLKPRDLQIAKSL+SSKILQENYRQELTLMVERGQKAELE
Sbjct: 301 VKWLGVRGDDLQLIPQESLVPLKPRDLQIAKSLLSSKILQENYRQELTLMVERGQKAELE 360

Query: 361 ALYHNGFDYLEKYIVKKIIQFSYI 385
           ALYHNGFDYLEKYIVKKIIQFSYI
Sbjct: 361 ALYHNGFDYLEKYIVKKIIQFSYI 384

BLAST of Cmc09g0258291 vs. ExPASy TrEMBL
Match: A0A6J1H3X3 (meiotic recombination protein SPO11-2 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111459771 PE=3 SV=1)

HSP 1 Score: 704.5 bits (1817), Expect = 2.4e-199
Identity = 345/384 (89.84%), Postives = 371/384 (96.61%), Query Frame = 0

Query: 1   MEPAGLFESSMRFFSDQELCYADILTPQEVVARIQIAVLNFLRILNSSSPAISNLPLIDR 60
           ME  GLF+SSMRFFSDQELCYADILTPQEV+ARIQ+AVLNFLRIL+SS+PAISNLPLIDR
Sbjct: 1   METTGLFKSSMRFFSDQELCYADILTPQEVLARIQVAVLNFLRILSSSTPAISNLPLIDR 60

Query: 61  RSSNSRVSQGILTDDSWIFLSHSFCTRSLMRPNASKAFVRVWKLMEMCSQILIQDKRVTQ 120
            SSNSRV+QGILTD+SWIFLSHSFCTRSLMR NA+KAFVRVWKLMEMCSQIL+Q+KRVTQ
Sbjct: 61  SSSNSRVNQGILTDESWIFLSHSFCTRSLMRANAAKAFVRVWKLMEMCSQILLQEKRVTQ 120

Query: 121 RELFYKLLCDSPAYFSTQLHVNRTIQDVVALLQCSRYSLGIMASSRGLVAGRLLLQEPEQ 180
           RELFYKL CDSPAYF TQLHVNRTIQDVVALL+CSR+SLG+MASSRGLVAGRLLLQEPEQ
Sbjct: 121 RELFYKLFCDSPAYFPTQLHVNRTIQDVVALLRCSRFSLGVMASSRGLVAGRLLLQEPEQ 180

Query: 181 EVVDCTACGSSGYPISGDLDLLKMLTLKTDARYIIVIEKHAIFQRLADDRVFNHIPSILI 240
           E VDC ACG+SGY ISGDLDLL+ LTLKTDARY+IVIEKHAIFQRLA+DRVFNHIPSILI
Sbjct: 181 ETVDCAACGTSGYAISGDLDLLQKLTLKTDARYVIVIEKHAIFQRLAEDRVFNHIPSILI 240

Query: 241 TAKGYPDLATRYFLHKIGKTFPHLPMFGLVDWNPAGLAILCTFKYGSIGMGLEAYRYACN 300
           TAKGYPD+ATRYFLH+I KTFPHLP+FGLVDWNPAGLAILCTFKYGSIGMGLEAYRYACN
Sbjct: 241 TAKGYPDMATRYFLHRISKTFPHLPIFGLVDWNPAGLAILCTFKYGSIGMGLEAYRYACN 300

Query: 301 VKWLGVRGDDLQLIPQESLVPLKPRDLQIAKSLMSSKILQENYRQELTLMVERGQKAELE 360
           VKWLG+RGDD+QLIPQESLV LKPRDLQIAKSLMSS+ILQENYR+ELTLMVE GQKAELE
Sbjct: 301 VKWLGMRGDDVQLIPQESLVSLKPRDLQIAKSLMSSEILQENYREELTLMVEGGQKAELE 360

Query: 361 ALYHNGFDYLEKYIVKKIIQFSYI 385
           ALYHNGFDYLEKY+VKKIIQF+YI
Sbjct: 361 ALYHNGFDYLEKYLVKKIIQFNYI 384

BLAST of Cmc09g0258291 vs. ExPASy TrEMBL
Match: A0A6J1K4Y3 (meiotic recombination protein SPO11-2 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111491232 PE=3 SV=1)

HSP 1 Score: 697.2 bits (1798), Expect = 3.8e-197
Identity = 342/384 (89.06%), Postives = 368/384 (95.83%), Query Frame = 0

Query: 1   MEPAGLFESSMRFFSDQELCYADILTPQEVVARIQIAVLNFLRILNSSSPAISNLPLIDR 60
           ME  GLF+SSMRFFSDQELCYADILTPQE +ARIQ+AVLNFLRIL+SS+PAISNLPLIDR
Sbjct: 1   METTGLFKSSMRFFSDQELCYADILTPQEALARIQVAVLNFLRILSSSTPAISNLPLIDR 60

Query: 61  RSSNSRVSQGILTDDSWIFLSHSFCTRSLMRPNASKAFVRVWKLMEMCSQILIQDKRVTQ 120
            S+NSRV+QGILTD+SWIFLSHSF TRSLMR NA+KAFVRVWKLMEMCSQIL+Q+KRVTQ
Sbjct: 61  SSNNSRVNQGILTDESWIFLSHSFSTRSLMRANAAKAFVRVWKLMEMCSQILLQEKRVTQ 120

Query: 121 RELFYKLLCDSPAYFSTQLHVNRTIQDVVALLQCSRYSLGIMASSRGLVAGRLLLQEPEQ 180
           RELFYKLLCDSPAYF TQLHVNRTIQDVV LL+CSR+SLG+MASSRGLVAGRLLLQEPEQ
Sbjct: 121 RELFYKLLCDSPAYFPTQLHVNRTIQDVVTLLRCSRFSLGVMASSRGLVAGRLLLQEPEQ 180

Query: 181 EVVDCTACGSSGYPISGDLDLLKMLTLKTDARYIIVIEKHAIFQRLADDRVFNHIPSILI 240
           E VDC ACGSSGY ISGDLDLL+ LTLKTDARY+IVIEKHAIFQRL +DRVFNHIPSILI
Sbjct: 181 ETVDCAACGSSGYAISGDLDLLQKLTLKTDARYVIVIEKHAIFQRLVEDRVFNHIPSILI 240

Query: 241 TAKGYPDLATRYFLHKIGKTFPHLPMFGLVDWNPAGLAILCTFKYGSIGMGLEAYRYACN 300
           TAKGYPD+ATRYFLH+I KTFPHLP+FGLVDWNPAGLAILCTFKYGSIGMGLEAYRYACN
Sbjct: 241 TAKGYPDIATRYFLHRISKTFPHLPIFGLVDWNPAGLAILCTFKYGSIGMGLEAYRYACN 300

Query: 301 VKWLGVRGDDLQLIPQESLVPLKPRDLQIAKSLMSSKILQENYRQELTLMVERGQKAELE 360
           VKWLG+RGDD+QLIPQESLV LKPRDLQIAKSLMSS+ILQENYR+ELTLMVE GQKAELE
Sbjct: 301 VKWLGMRGDDVQLIPQESLVSLKPRDLQIAKSLMSSEILQENYREELTLMVEGGQKAELE 360

Query: 361 ALYHNGFDYLEKYIVKKIIQFSYI 385
           ALYHNGFDYL+KYIVKKIIQF+YI
Sbjct: 361 ALYHNGFDYLQKYIVKKIIQFNYI 384

BLAST of Cmc09g0258291 vs. TAIR 10
Match: AT1G63990.1 (sporulation 11-2 )

HSP 1 Score: 557.8 bits (1436), Expect = 7.0e-159
Identity = 274/383 (71.54%), Postives = 328/383 (85.64%), Query Frame = 0

Query: 2   EPAGLFESSMRFFSDQELCYADILTPQEVVARIQIAVLNFLRILNSSSPAISNLPLIDRR 61
           E +GL  SSM+FFSDQ L YADIL P E  ARI+++VLN LRILNS  PAIS+L LI+R+
Sbjct: 3   ESSGL--SSMKFFSDQHLSYADILLPHEARARIEVSVLNLLRILNSPDPAISDLSLINRK 62

Query: 62  SSNSRVSQGILTDDSWIFLSHSFCTRSLMRPNASKAFVRVWKLMEMCSQILIQDKRVTQR 121
            SNS +++GILTD S+IFLS SF   SL     +KAFVRVWK+ME+C QIL+Q+KRVTQR
Sbjct: 63  RSNSCINKGILTDVSYIFLSTSFTKSSLTNAKTAKAFVRVWKVMEICFQILLQEKRVTQR 122

Query: 122 ELFYKLLCDSPAYFSTQLHVNRTIQDVVALLQCSRYSLGIMASSRGLVAGRLLLQEPEQE 181
           ELFYKLLCDSP YFS+Q+ VNR++QDVVALL+CSRYSLGIMASSRGLVAGRL LQEP +E
Sbjct: 123 ELFYKLLCDSPDYFSSQIEVNRSVQDVVALLRCSRYSLGIMASSRGLVAGRLFLQEPGKE 182

Query: 182 VVDCTACGSSGYPISGDLDLLKMLTLKTDARYIIVIEKHAIFQRLADDRVFNHIPSILIT 241
            VDC+ACGSSG+ I+GDL+LL    ++TDARYII++EKHAIF RL +DRVFNHIP + IT
Sbjct: 183 AVDCSACGSSGFAITGDLNLLDNTIMRTDARYIIIVEKHAIFHRLVEDRVFNHIPCVFIT 242

Query: 242 AKGYPDLATRYFLHKIGKTFPHLPMFGLVDWNPAGLAILCTFKYGSIGMGLEAYRYACNV 301
           AKGYPD+ATR+FLH++  TFP LP+  LVDWNPAGLAILCTFK+GSIGMGLEAYRYACNV
Sbjct: 243 AKGYPDIATRFFLHRMSTTFPDLPILVLVDWNPAGLAILCTFKFGSIGMGLEAYRYACNV 302

Query: 302 KWLGVRGDDLQLIPQESLVPLKPRDLQIAKSLMSSKILQENYRQELTLMVERGQKAELEA 361
           KW+G+RGDDL LIP+ESLVPLKP+D QIAKSL+SSKILQENY +EL+LMV+ G++AE+EA
Sbjct: 303 KWIGLRGDDLNLIPEESLVPLKPKDSQIAKSLLSSKILQENYIEELSLMVQTGKRAEIEA 362

Query: 362 LYHNGFDYLEKYIVKKIIQFSYI 385
           LY +G++YL KYI  KI+Q  YI
Sbjct: 363 LYCHGYNYLGKYIATKIVQGKYI 383

BLAST of Cmc09g0258291 vs. TAIR 10
Match: AT5G02820.1 (Spo11/DNA topoisomerase VI, subunit A protein )

HSP 1 Score: 181.8 bits (460), Expect = 1.0e-45
Identity = 108/308 (35.06%), Postives = 182/308 (59.09%), Query Frame = 0

Query: 87  RSLMRPNASKAFVR----VWKLMEMCSQILIQDKRVTQRELFYKLLCDSPAYFSTQLHVN 146
           +S +RP AS + VR      +++ +  Q+ +++  VT+R+LFY         F  Q   +
Sbjct: 128 KSTLRPFASVSSVRKTTITTRILALIHQLCLRNIHVTKRDLFY----TDVKLFQDQTQSD 187

Query: 147 RTIQDVVALLQCSRYSLGIMASSRGLVAGRLLLQEPEQEVVDCTACGSSGYPISGDLDLL 206
             + DV  +L C+R SL ++A+ +G+V GRL+  +   +++DCT  G  G  I  ++D  
Sbjct: 188 AVLDDVSCMLGCTRSSLNVIAAEKGVVVGRLIFSD-NGDMIDCTKMGMGGKAIPPNID-- 247

Query: 207 KMLTLKTDARYIIVIEKHAIFQRLADDRVFNHIPSILITAKGYPDLATRYFLHKIGKTFP 266
           ++  +++DA +I+++EK A + RLA+DR +N  P I++TAKG PD+ATR FL K+ K   
Sbjct: 248 RVGDMQSDAMFILLVEKDAAYMRLAEDRFYNRFPCIIVTAKGQPDVATRLFLRKM-KMEL 307

Query: 267 HLPMFGLVDWNPAGLAILCTFKYGSIGMGLEAYRYAC-NVKWLGVRGDDLQ--LIPQESL 326
            LP+  LVD +P GL IL  +  GS  M  ++      ++KWLG+R  DL    IP++  
Sbjct: 308 KLPVLALVDSDPYGLKILSVYGCGSKNMSYDSANLTTPDIKWLGIRPSDLDKYKIPEQCR 367

Query: 327 VPLKPRDLQIAKSLMSSKILQEN--YRQELTLMVERGQKAELEALYHNGFDYL-EKYIVK 385
           +P+  +D++  K ++    +++N  + +EL LMV+  QKAE++AL   GF YL E Y+  
Sbjct: 368 LPMTEQDIKTGKDMLEEDFVKKNPGWVEELNLMVKTKQKAEIQALSSFGFQYLSEVYLPL 427

BLAST of Cmc09g0258291 vs. TAIR 10
Match: AT3G13170.1 (Spo11/DNA topoisomerase VI, subunit A protein )

HSP 1 Score: 149.4 bits (376), Expect = 5.7e-36
Identity = 96/284 (33.80%), Postives = 166/284 (58.45%), Query Frame = 0

Query: 103 KLMEMCSQILIQDKRVTQRELFYKLLCDSPAYFSTQLHVNRTIQDVVALLQCSRYSLGIM 162
           +++ +  Q+L +++  ++R+++Y      P+ F  Q  V+R I D+  L QCSRY+L ++
Sbjct: 81  RVLLIVQQLLQENRHASKRDIYYM----HPSAFKAQSIVDRAIGDICILFQCSRYNLNVV 140

Query: 163 ASSRGLVAGRLLLQEPEQEVVDCTACGSSGYPISGDLDLLKMLTLKTDARYIIVIEKHAI 222
           +   GLV G L  +E  ++  DC    ++ YP+   ++  ++  + + A YI+V+EK  +
Sbjct: 141 SVGNGLVMGWLKFREAGRK-FDCLNSLNTAYPVPVLVE--EVEDIVSLAEYILVVEKETV 200

Query: 223 FQRLADDRVFNHIPSILITAKGYPDLATRYFLHKIGKTFPHLPMFGLVDWNPAGLAILCT 282
           FQRLA+D        I+IT +GYPD++TR FL  + +   HLP+  LVD +P G  IL T
Sbjct: 201 FQRLANDMFCKTNRCIVITGRGYPDVSTRRFLRLLMEKL-HLPVHCLVDCDPYGFEILAT 260

Query: 283 FKYGSIGMG--LEAYRYACNVKWLGVRGDDLQL--IPQESLVPLKPRDLQIAKSLMSSKI 342
           +++GS+ M   +E+ R A ++KWLG    D ++  +P++ L+PL   D +  ++++    
Sbjct: 261 YRFGSMQMAYDIESLR-APDMKWLGAFPSDSEVYSVPKQCLLPLTEEDKKRTEAMLLRCY 320

Query: 343 LQE---NYRQELTLMVERGQKAELEALYHNGFDYL-EKYIVKKI 379
           L+     +R EL  M++RG K E+EAL  +   +L E YI  KI
Sbjct: 321 LKREMPQWRLELETMLKRGVKFEIEALSVHSLSFLSEVYIPSKI 355

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_008437337.11.8e-217100.00PREDICTED: meiotic recombination protein SPO11-2 [Cucumis melo] >XP_008437338.1 ... [more]
KAA0042721.18.4e-21597.21meiotic recombination protein SPO11-2 [Cucumis melo var. makuwa][more]
XP_004143970.13.9e-21297.66meiotic recombination protein SPO11-2 [Cucumis sativus] >XP_011654717.1 meiotic ... [more]
XP_038906472.12.7e-20594.53meiotic recombination protein SPO11-2 isoform X1 [Benincasa hispida] >XP_0389064... [more]
XP_023533741.17.7e-20090.36meiotic recombination protein SPO11-2 isoform X1 [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
Q9M4A19.8e-15871.54Meiotic recombination protein SPO11-2 OS=Arabidopsis thaliana OX=3702 GN=SPO11-2... [more]
Q5ZPI92.5e-13763.21Meiotic recombination protein SPO11-2 OS=Oryza sativa subsp. indica OX=39946 GN=... [more]
Q6ZD955.6e-13762.95Meiotic recombination protein SPO11-2 OS=Oryza sativa subsp. japonica OX=39947 G... [more]
Q9LZ031.5e-4435.06DNA topoisomerase 6 subunit A OS=Arabidopsis thaliana OX=3702 GN=TOP6A PE=1 SV=1[more]
Q9Y5K11.9e-4432.34Meiotic recombination protein SPO11 OS=Homo sapiens OX=9606 GN=SPO11 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A1S3AUC68.8e-218100.00meiotic recombination protein SPO11-2 OS=Cucumis melo OX=3656 GN=LOC103482788 PE... [more]
A0A5A7TMD54.1e-21597.21Meiotic recombination protein SPO11-2 OS=Cucumis melo var. makuwa OX=1194695 GN=... [more]
A0A0A0KNU21.9e-21297.66TP6A_N domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G152220 PE=3... [more]
A0A6J1H3X32.4e-19989.84meiotic recombination protein SPO11-2 isoform X1 OS=Cucurbita moschata OX=3662 G... [more]
A0A6J1K4Y33.8e-19789.06meiotic recombination protein SPO11-2 isoform X1 OS=Cucurbita maxima OX=3661 GN=... [more]
Match NameE-valueIdentityDescription
AT1G63990.17.0e-15971.54sporulation 11-2 [more]
AT5G02820.11.0e-4535.06Spo11/DNA topoisomerase VI, subunit A protein [more]
AT3G13170.15.7e-3633.80Spo11/DNA topoisomerase VI, subunit A protein [more]
InterPro
Analysis Name: InterPro Annotations of Melon (Charmono) v1.1
Date Performed: 2022-10-13
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR013048Meiotic recombination, Spo11PRINTSPR01551SPO11HOMOLOGcoord: 228..243
score: 33.93
coord: 276..295
score: 50.0
coord: 85..101
score: 21.01
coord: 130..150
score: 23.81
IPR002815Spo11/DNA topoisomerase VI subunit APRINTSPR01550TOP6AFAMILYcoord: 238..259
score: 45.74
coord: 214..231
score: 44.44
coord: 138..155
score: 27.78
coord: 271..287
score: 48.53
coord: 156..171
score: 45.31
IPR002815Spo11/DNA topoisomerase VI subunit APANTHERPTHR10848MEIOTIC RECOMBINATION PROTEIN SPO11coord: 16..383
IPR013049Spo11/DNA topoisomerase VI, subunit A, N-terminalPFAMPF04406TP6A_Ncoord: 96..161
e-value: 1.3E-15
score: 57.1
IPR036388Winged helix-like DNA-binding domain superfamilyGENE3D1.10.10.10coord: 18..164
e-value: 1.4E-21
score: 78.8
NoneNo IPR availableGENE3D3.40.1360.10coord: 165..384
e-value: 4.2E-65
score: 221.4
NoneNo IPR availablePANTHERPTHR10848:SF0MEIOTIC RECOMBINATION PROTEIN SPO11coord: 16..383
IPR034136Topoisomerase 6 subunit A/Spo11, TOPRIM domainCDDcd00223TOPRIM_TopoIIB_SPOcoord: 212..369
e-value: 4.97413E-60
score: 189.773
IPR036078Spo11/DNA topoisomerase VI subunit A superfamilySUPERFAMILY56726DNA topoisomerase IV, alpha subunitcoord: 96..384

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cmc09g0258291.1Cmc09g0258291.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0051026 chiasma assembly
biological_process GO:0009553 embryo sac development
biological_process GO:0042138 meiotic DNA double-strand break formation
biological_process GO:0000706 meiotic DNA double-strand break processing
biological_process GO:0009555 pollen development
biological_process GO:0048316 seed development
biological_process GO:0006259 DNA metabolic process
cellular_component GO:0000228 nuclear chromosome
cellular_component GO:0005694 chromosome
molecular_function GO:0005524 ATP binding
molecular_function GO:0003677 DNA binding
molecular_function GO:0003918 DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity
molecular_function GO:0016787 hydrolase activity
molecular_function GO:0046872 metal ion binding
molecular_function GO:0003824 catalytic activity