Homology
BLAST of Cmc09g0257051 vs. NCBI nr
Match:
KAA0042818.1 (subtilisin-like protease SBT1.7 [Cucumis melo var. makuwa])
HSP 1 Score: 1478.8 bits (3827), Expect = 0.0e+00
Identity = 745/745 (100.00%), Postives = 745/745 (100.00%), Query Frame = 0
Query: 1 MVLLPSLFLLLLLNFHGYEAQVTELPLSNLHTYIVHVKKPEVVDDLEIWHRSFLPTSLDN 60
MVLLPSLFLLLLLNFHGYEAQVTELPLSNLHTYIVHVKKPEVVDDLEIWHRSFLPTSLDN
Sbjct: 1 MVLLPSLFLLLLLNFHGYEAQVTELPLSNLHTYIVHVKKPEVVDDLEIWHRSFLPTSLDN 60
Query: 61 EEEQPTLLYSYRNVMSGFSARLTEEHVKAMEEKDGFVSARRETIVHLHTTHTPDFLGLNR 120
EEEQPTLLYSYRNVMSGFSARLTEEHVKAMEEKDGFVSARRETIVHLHTTHTPDFLGLNR
Sbjct: 61 EEEQPTLLYSYRNVMSGFSARLTEEHVKAMEEKDGFVSARRETIVHLHTTHTPDFLGLNR 120
Query: 121 QFGFWKDSNFGKGVIIGVLDGGITPNHPSFDDAGMPQPPAKWKGRCEFNFSACNNKLIGA 180
QFGFWKDSNFGKGVIIGVLDGGITPNHPSFDDAGMPQPPAKWKGRCEFNFSACNNKLIGA
Sbjct: 121 QFGFWKDSNFGKGVIIGVLDGGITPNHPSFDDAGMPQPPAKWKGRCEFNFSACNNKLIGA 180
Query: 181 RSMNLASQALKGKITTLDDSPIDEDGHGTHTASTAAGTFVDGAEALGNAFGTAVGMAPLA 240
RSMNLASQALKGKITTLDDSPIDEDGHGTHTASTAAGTFVDGAEALGNAFGTAVGMAPLA
Sbjct: 181 RSMNLASQALKGKITTLDDSPIDEDGHGTHTASTAAGTFVDGAEALGNAFGTAVGMAPLA 240
Query: 241 HLAIYKVCFGEDCSDVDILAGLDAAVEDGVDVLSISLGGPSVPFFADITAIGSFAAIQKG 300
HLAIYKVCFGEDCSDVDILAGLDAAVEDGVDVLSISLGGPSVPFFADITAIGSFAAIQKG
Sbjct: 241 HLAIYKVCFGEDCSDVDILAGLDAAVEDGVDVLSISLGGPSVPFFADITAIGSFAAIQKG 300
Query: 301 IFVSCSAANSGPFNATLSNEAPWILTVAASTIDRKITATAKLGNGEEFDGESLFQPNDFP 360
IFVSCSAANSGPFNATLSNEAPWILTVAASTIDRKITATAKLGNGEEFDGESLFQPNDFP
Sbjct: 301 IFVSCSAANSGPFNATLSNEAPWILTVAASTIDRKITATAKLGNGEEFDGESLFQPNDFP 360
Query: 361 QTLLPLVFPGEKNETVALCAEGSLKNIDVKGKVVVCERGGGIARIAKGVEVKNGGGAAMI 420
QTLLPLVFPGEKNETVALCAEGSLKNIDVKGKVVVCERGGGIARIAKGVEVKNGGGAAMI
Sbjct: 361 QTLLPLVFPGEKNETVALCAEGSLKNIDVKGKVVVCERGGGIARIAKGVEVKNGGGAAMI 420
Query: 421 LLNAESDGFTTEVDAHVLPASHVSHTAALKIKAYINSTTYPTATILFKGTTIGDDFSPAI 480
LLNAESDGFTTEVDAHVLPASHVSHTAALKIKAYINSTTYPTATILFKGTTIGDDFSPAI
Sbjct: 421 LLNAESDGFTTEVDAHVLPASHVSHTAALKIKAYINSTTYPTATILFKGTTIGDDFSPAI 480
Query: 481 ASFSSRGPSLASPGILKPDITGPGVSILAAWPFPLDNNTNTKSTFNIISGTSMSCPHLSG 540
ASFSSRGPSLASPGILKPDITGPGVSILAAWPFPLDNNTNTKSTFNIISGTSMSCPHLSG
Sbjct: 481 ASFSSRGPSLASPGILKPDITGPGVSILAAWPFPLDNNTNTKSTFNIISGTSMSCPHLSG 540
Query: 541 IAALIKSAHPDWSPAAIKSSIMTTANITNLEGNPILDETLQPADLFAIGAGHVNPSKAID 600
IAALIKSAHPDWSPAAIKSSIMTTANITNLEGNPILDETLQPADLFAIGAGHVNPSKAID
Sbjct: 541 IAALIKSAHPDWSPAAIKSSIMTTANITNLEGNPILDETLQPADLFAIGAGHVNPSKAID 600
Query: 601 PGLVYDIQPDDYIPYLCGLGYTNNQVSLIAHKPIDCLTTSSIPEGELNYPSFMVKLGPVQ 660
PGLVYDIQPDDYIPYLCGLGYTNNQVSLIAHKPIDCLTTSSIPEGELNYPSFMVKLGPVQ
Sbjct: 601 PGLVYDIQPDDYIPYLCGLGYTNNQVSLIAHKPIDCLTTSSIPEGELNYPSFMVKLGPVQ 660
Query: 661 TFSRTVTSVGSGRVVYNVVIEAPEGVSVTVRPRKLSFSALNQKATYSVTFKRIGSISPSI 720
TFSRTVTSVGSGRVVYNVVIEAPEGVSVTVRPRKLSFSALNQKATYSVTFKRIGSISPSI
Sbjct: 661 TFSRTVTSVGSGRVVYNVVIEAPEGVSVTVRPRKLSFSALNQKATYSVTFKRIGSISPSI 720
Query: 721 EFAEGYLKWVSAKHVVRSPISVKFV 746
EFAEGYLKWVSAKHVVRSPISVKFV
Sbjct: 721 EFAEGYLKWVSAKHVVRSPISVKFV 745
BLAST of Cmc09g0257051 vs. NCBI nr
Match:
XP_008437181.1 (PREDICTED: subtilisin-like protease SBT1.7 [Cucumis melo])
HSP 1 Score: 1473.4 bits (3813), Expect = 0.0e+00
Identity = 743/745 (99.73%), Postives = 743/745 (99.73%), Query Frame = 0
Query: 1 MVLLPSLFLLLLLNFHGYEAQVTELPLSNLHTYIVHVKKPEVVDDLEIWHRSFLPTSLDN 60
MVLLPSLFLLLLLNFHGYEAQVTELPLSNLHTYIVHVKKPEVVDDLEIWHRSFLPTSLDN
Sbjct: 1 MVLLPSLFLLLLLNFHGYEAQVTELPLSNLHTYIVHVKKPEVVDDLEIWHRSFLPTSLDN 60
Query: 61 EEEQPTLLYSYRNVMSGFSARLTEEHVKAMEEKDGFVSARRETIVHLHTTHTPDFLGLNR 120
EEEQPTLLYSYRNVMSGFSARLTEEHVKAMEEKDGFVSARRETIVHLHTTHTPDFLGLNR
Sbjct: 61 EEEQPTLLYSYRNVMSGFSARLTEEHVKAMEEKDGFVSARRETIVHLHTTHTPDFLGLNR 120
Query: 121 QFGFWKDSNFGKGVIIGVLDGGITPNHPSFDDAGMPQPPAKWKGRCEFNFSACNNKLIGA 180
QFGFWKDSNFGKGVIIGVLDGGITPNHPSFDDAGM QPPAKWKGRCEFNFSACNNKLIGA
Sbjct: 121 QFGFWKDSNFGKGVIIGVLDGGITPNHPSFDDAGMAQPPAKWKGRCEFNFSACNNKLIGA 180
Query: 181 RSMNLASQALKGKITTLDDSPIDEDGHGTHTASTAAGTFVDGAEALGNAFGTAVGMAPLA 240
RSMNLASQALKGKITTLDDSPIDEDGHGTHTASTAAGTFVDGAEALGNAFGTAVGMAPLA
Sbjct: 181 RSMNLASQALKGKITTLDDSPIDEDGHGTHTASTAAGTFVDGAEALGNAFGTAVGMAPLA 240
Query: 241 HLAIYKVCFGEDCSDVDILAGLDAAVEDGVDVLSISLGGPSVPFFADITAIGSFAAIQKG 300
HLAIYKVCFGEDCSDVDILAGLDAAVEDGVDVLSISLGGPSVPFFADITAIGSFAAIQKG
Sbjct: 241 HLAIYKVCFGEDCSDVDILAGLDAAVEDGVDVLSISLGGPSVPFFADITAIGSFAAIQKG 300
Query: 301 IFVSCSAANSGPFNATLSNEAPWILTVAASTIDRKITATAKLGNGEEFDGESLFQPNDFP 360
IFVSCSAANSGPFNATLSNEAPWILTVAASTIDRKITATAKLGNGEEFDGESLFQPNDFP
Sbjct: 301 IFVSCSAANSGPFNATLSNEAPWILTVAASTIDRKITATAKLGNGEEFDGESLFQPNDFP 360
Query: 361 QTLLPLVFPGEKNETVALCAEGSLKNIDVKGKVVVCERGGGIARIAKGVEVKNGGGAAMI 420
QTLLPLVFPGEKNETVALCAEGSLKNIDVKGKVVVCERGGGIARIAKGVEVKNGGGAAMI
Sbjct: 361 QTLLPLVFPGEKNETVALCAEGSLKNIDVKGKVVVCERGGGIARIAKGVEVKNGGGAAMI 420
Query: 421 LLNAESDGFTTEVDAHVLPASHVSHTAALKIKAYINSTTYPTATILFKGTTIGDDFSPAI 480
LLNAESDGFTTEVDAHVLPASHVSHTAALKIKAYINSTTYPTATILFKGTTIGDDFSPAI
Sbjct: 421 LLNAESDGFTTEVDAHVLPASHVSHTAALKIKAYINSTTYPTATILFKGTTIGDDFSPAI 480
Query: 481 ASFSSRGPSLASPGILKPDITGPGVSILAAWPFPLDNNTNTKSTFNIISGTSMSCPHLSG 540
ASFSSRGPSLASPGILKPDITGPGVSILAAWPFPLDNNTNTKSTFNIISGTSMSCPHLSG
Sbjct: 481 ASFSSRGPSLASPGILKPDITGPGVSILAAWPFPLDNNTNTKSTFNIISGTSMSCPHLSG 540
Query: 541 IAALIKSAHPDWSPAAIKSSIMTTANITNLEGNPILDETLQPADLFAIGAGHVNPSKAID 600
IAALIKSAHPDWSPAAIKSSIMTTANITNLEGNPILDETLQPADLFAIGAGHVNPSKAID
Sbjct: 541 IAALIKSAHPDWSPAAIKSSIMTTANITNLEGNPILDETLQPADLFAIGAGHVNPSKAID 600
Query: 601 PGLVYDIQPDDYIPYLCGLGYTNNQVSLIAHKPIDCLTTSSIPEGELNYPSFMVKLGPVQ 660
PGLVYDIQPDDYIPYLCGLGYTNNQVSLIAHKPIDCLTTSSIPEGELNYPSFMVKLGPVQ
Sbjct: 601 PGLVYDIQPDDYIPYLCGLGYTNNQVSLIAHKPIDCLTTSSIPEGELNYPSFMVKLGPVQ 660
Query: 661 TFSRTVTSVGSGRVVYNVVIEAPEGVSVTVRPRKLSFSALNQKATYSVTFKRIGSISPSI 720
TFSRTVTSVGSGRVVYNVVIEAPEGVSVTVRPRKLSFSALNQKATYSVTFKR GSISPSI
Sbjct: 661 TFSRTVTSVGSGRVVYNVVIEAPEGVSVTVRPRKLSFSALNQKATYSVTFKRSGSISPSI 720
Query: 721 EFAEGYLKWVSAKHVVRSPISVKFV 746
EFAEGYLKWVSAKHVVRSPISVKFV
Sbjct: 721 EFAEGYLKWVSAKHVVRSPISVKFV 745
BLAST of Cmc09g0257051 vs. NCBI nr
Match:
KGN50165.1 (hypothetical protein Csa_000664 [Cucumis sativus])
HSP 1 Score: 1423.3 bits (3683), Expect = 0.0e+00
Identity = 713/745 (95.70%), Postives = 730/745 (97.99%), Query Frame = 0
Query: 1 MVLLPSLFLLLLLNFHGYEAQVTELPLSNLHTYIVHVKKPEVVDDLEIWHRSFLPTSLDN 60
MVLLPSLFLLLLLNFH YEAQVTELP SNLHTYIVHVKKPEVVDDLE WHRSFLPTSL+N
Sbjct: 1 MVLLPSLFLLLLLNFHVYEAQVTELPFSNLHTYIVHVKKPEVVDDLESWHRSFLPTSLEN 60
Query: 61 EEEQPTLLYSYRNVMSGFSARLTEEHVKAMEEKDGFVSARRETIVHLHTTHTPDFLGLNR 120
EEQPTLLYSYRNVMSGFSARLTEEHVKAMEEKDGFVSARRETIVHLHTTH+P+FLGLNR
Sbjct: 61 SEEQPTLLYSYRNVMSGFSARLTEEHVKAMEEKDGFVSARRETIVHLHTTHSPNFLGLNR 120
Query: 121 QFGFWKDSNFGKGVIIGVLDGGITPNHPSFDDAGMPQPPAKWKGRCEFNFSACNNKLIGA 180
QFGFWKDSNFGKGVIIGVLDGGITP+HPSF DAGMPQPPAKWKGRCEFNFSACNNKLIGA
Sbjct: 121 QFGFWKDSNFGKGVIIGVLDGGITPSHPSFVDAGMPQPPAKWKGRCEFNFSACNNKLIGA 180
Query: 181 RSMNLASQALKGKITTLDDSPIDEDGHGTHTASTAAGTFVDGAEALGNAFGTAVGMAPLA 240
RS+NLASQALKGKITTLDDSPIDEDGHGTHTASTAAGTFVDGAEALGNAFGTAVGMAPLA
Sbjct: 181 RSLNLASQALKGKITTLDDSPIDEDGHGTHTASTAAGTFVDGAEALGNAFGTAVGMAPLA 240
Query: 241 HLAIYKVCFGEDCSDVDILAGLDAAVEDGVDVLSISLGGPSVPFFADITAIGSFAAIQKG 300
HLAIYKVCFGE CS+VDILAGLDAAVEDGVDVLSISLGGP VPFFADITAIG+FAAIQKG
Sbjct: 241 HLAIYKVCFGESCSNVDILAGLDAAVEDGVDVLSISLGGPPVPFFADITAIGAFAAIQKG 300
Query: 301 IFVSCSAANSGPFNATLSNEAPWILTVAASTIDRKITATAKLGNGEEFDGESLFQPNDFP 360
IFVSCSAANSGPFNATLSNEAPWILTVAASTIDRKITATAKLGNGEEFDGESLFQPNDFP
Sbjct: 301 IFVSCSAANSGPFNATLSNEAPWILTVAASTIDRKITATAKLGNGEEFDGESLFQPNDFP 360
Query: 361 QTLLPLVFPGEKNETVALCAEGSLKNIDVKGKVVVCERGGGIARIAKGVEVKNGGGAAMI 420
QT LPLVFPGEKNETVALCAEGSLKNIDVKGKVVVC+RGGGIARIAKGVEVKN GGAAMI
Sbjct: 361 QTFLPLVFPGEKNETVALCAEGSLKNIDVKGKVVVCDRGGGIARIAKGVEVKNAGGAAMI 420
Query: 421 LLNAESDGFTTEVDAHVLPASHVSHTAALKIKAYINSTTYPTATILFKGTTIGDDFSPAI 480
LLNAESDGFTTE DAHVLPASHVSHTAALKIKAYINSTTYPTATI+FKGTTIGDDFSPAI
Sbjct: 421 LLNAESDGFTTEADAHVLPASHVSHTAALKIKAYINSTTYPTATIVFKGTTIGDDFSPAI 480
Query: 481 ASFSSRGPSLASPGILKPDITGPGVSILAAWPFPLDNNTNTKSTFNIISGTSMSCPHLSG 540
A+FSSRGPSLASPGILKPDITGPGVSILAAWPFPLDNNTNTKSTFNI+SGTSMSCPHLSG
Sbjct: 481 AAFSSRGPSLASPGILKPDITGPGVSILAAWPFPLDNNTNTKSTFNIVSGTSMSCPHLSG 540
Query: 541 IAALIKSAHPDWSPAAIKSSIMTTANITNLEGNPILDETLQPADLFAIGAGHVNPSKAID 600
IAALIKSAHPDWSPAAIKSSIMTTANITNLEGNPI+D+TLQPADLFAIGAGHVNPSKA+D
Sbjct: 541 IAALIKSAHPDWSPAAIKSSIMTTANITNLEGNPIVDQTLQPADLFAIGAGHVNPSKAVD 600
Query: 601 PGLVYDIQPDDYIPYLCGLGYTNNQVSLIAHKPIDCLTTSSIPEGELNYPSFMVKLGPVQ 660
PGLVYDIQPDDYIPYLCGLGYTNNQVSLIAHKPIDCLTT+SIPEGELNYPSFMVKLG VQ
Sbjct: 601 PGLVYDIQPDDYIPYLCGLGYTNNQVSLIAHKPIDCLTTTSIPEGELNYPSFMVKLGQVQ 660
Query: 661 TFSRTVTSVGSGRVVYNVVIEAPEGVSVTVRPRKLSFSALNQKATYSVTFKRIGSISPSI 720
TFSRTVT VGSGR VYNVVIEAPEGVSVTVRPRK+ FSALNQKATYSVTFKRIGSISPS
Sbjct: 661 TFSRTVTYVGSGREVYNVVIEAPEGVSVTVRPRKVIFSALNQKATYSVTFKRIGSISPST 720
Query: 721 EFAEGYLKWVSAKHVVRSPISVKFV 746
EFAEGYLKWVSAKH+VRSPISVKFV
Sbjct: 721 EFAEGYLKWVSAKHLVRSPISVKFV 745
BLAST of Cmc09g0257051 vs. NCBI nr
Match:
XP_038876087.1 (LOW QUALITY PROTEIN: subtilisin-like protease 4 [Benincasa hispida])
HSP 1 Score: 1298.5 bits (3359), Expect = 0.0e+00
Identity = 642/722 (88.92%), Postives = 670/722 (92.80%), Query Frame = 0
Query: 24 ELPLSNLHTYIVHVKKPEVVDDLEIWHRSFLPTSLDNEEEQPTLLYSYRNVMSGFSARLT 83
+LPL LHTYIVHVKKPE DDLE WHRSFLPTSL+N EEQP+LLYSYRNVMSGF+ARLT
Sbjct: 23 KLPLC-LHTYIVHVKKPETADDLESWHRSFLPTSLENSEEQPSLLYSYRNVMSGFAARLT 82
Query: 84 EEHVKAMEEKDGFVSARRETIVHLHTTHTPDFLGLNRQFGFWKDSNFGKGVIIGVLDGGI 143
EE VKAMEEKDGFVSARRE I+HLHTTHTPDFLGLNRQFGFWKDSNFGKGVIIGVLDGGI
Sbjct: 83 EEQVKAMEEKDGFVSARREKILHLHTTHTPDFLGLNRQFGFWKDSNFGKGVIIGVLDGGI 142
Query: 144 TPNHPSFDDAGMPQPPAKWKGRCEFNFSACNNKLIGARSMNLASQALKGKITTLDDSPID 203
TPNHPSFDD GMPQPP KWKGRCEFNFSACNNKLIGARS NLAS+ALKG TT+DDSPID
Sbjct: 143 TPNHPSFDDVGMPQPPTKWKGRCEFNFSACNNKLIGARSFNLASKALKGHATTMDDSPID 202
Query: 204 EDGHGTHTASTAAGTFVDGAEALGNAFGTAVGMAPLAHLAIYKVCFGEDCSDVDILAGLD 263
EDGHGTHTASTAAGTF+DGAEALGNA GTA GMAPLAHLAIYKVCFGEDC D DILA LD
Sbjct: 203 EDGHGTHTASTAAGTFIDGAEALGNAIGTAXGMAPLAHLAIYKVCFGEDCPDTDILAALD 262
Query: 264 AAVEDGVDVLSISLGGPSVPFFADITAIGSFAAIQKGIFVSCSAANSGPFNATLSNEAPW 323
AAVEDGVDVLS+SLG PS PFF D AIG+F AIQKGIFV CSAANSGPFNATLSNEAPW
Sbjct: 263 AAVEDGVDVLSLSLGSPSAPFFEDSIAIGAFTAIQKGIFVRCSAANSGPFNATLSNEAPW 322
Query: 324 ILTVAASTIDRKITATAKLGNGEEFDGESLFQPNDFPQTLLPLVFPGEKNETVALCAEGS 383
ILTVAASTIDR+I A AKLGNGEE DGESLFQPNDFPQT LPLVFPGEKNETVALC EGS
Sbjct: 323 ILTVAASTIDRRIVAAAKLGNGEELDGESLFQPNDFPQTFLPLVFPGEKNETVALCGEGS 382
Query: 384 LKNIDVKGKVVVCERGGGIARIAKGVEVKNGGGAAMILLNAESDGFTTEVDAHVLPASHV 443
LKNIDVKGKVVVCERGGGIARIAKGVEVKN GGAAMILLN ESDGFTTE DAHVLPASHV
Sbjct: 383 LKNIDVKGKVVVCERGGGIARIAKGVEVKNAGGAAMILLNTESDGFTTEADAHVLPASHV 442
Query: 444 SHTAALKIKAYINSTTYPTATILFKGTTIGDDFSPAIASFSSRGPSLASPGILKPDITGP 503
SHTAALKIKAYINSTTYP ATI+FKGT IGDDFSPAIASFSSRGPSLASPGILKPDITGP
Sbjct: 443 SHTAALKIKAYINSTTYPKATIVFKGTIIGDDFSPAIASFSSRGPSLASPGILKPDITGP 502
Query: 504 GVSILAAWPFPLDNNTNTKSTFNIISGTSMSCPHLSGIAALIKSAHPDWSPAAIKSSIMT 563
GVSILAAWPFPLDNNTN K TFNI+SGTSMSCPHLSGIAALIKS+HPDWSPA IKSSIMT
Sbjct: 503 GVSILAAWPFPLDNNTNPKXTFNIVSGTSMSCPHLSGIAALIKSSHPDWSPAVIKSSIMT 562
Query: 564 TANITNLEGNPILDETLQPADLFAIGAGHVNPSKAIDPGLVYDIQPDDYIPYLCGLGYTN 623
TANITNLEGNPI+D+TLQPADLFA GAGHVNPSKA DPGLVYDIQPDDYIPYLCGLGY N
Sbjct: 563 TANITNLEGNPIVDQTLQPADLFATGAGHVNPSKANDPGLVYDIQPDDYIPYLCGLGYKN 622
Query: 624 NQVSLIAHKPIDCLTTSSIPEGELNYPSFMVKLGPVQTFSRTVTSVGSGRVVYNVVIEAP 683
NQVSL+AHKP++CLTT SIPEGELNYPSF V+LGP QTF+RTVT+VG GR VY VV+EAP
Sbjct: 623 NQVSLVAHKPVECLTTPSIPEGELNYPSFTVELGPPQTFTRTVTNVGGGREVYTVVVEAP 682
Query: 684 EGVSVTVRPRKLSFSALNQKATYSVTFKRIGSISPSIEFAEGYLKWVSAKHVVRSPISVK 743
VSVTV+PR L FSALNQKATY+VTFKRIGSISPSIEFA+GY+KWVS KHVVRSPIS+K
Sbjct: 683 AAVSVTVKPRTLIFSALNQKATYAVTFKRIGSISPSIEFAKGYIKWVSTKHVVRSPISIK 742
Query: 744 FV 746
FV
Sbjct: 743 FV 743
BLAST of Cmc09g0257051 vs. NCBI nr
Match:
XP_022958542.1 (subtilisin-like protease SBT1.8 [Cucurbita moschata])
HSP 1 Score: 1236.9 bits (3199), Expect = 0.0e+00
Identity = 623/745 (83.62%), Postives = 663/745 (88.99%), Query Frame = 0
Query: 1 MVLLPSLFLLLLLNFHGYEAQVTELPLSNLHTYIVHVKKPEVVDDLEIWHRSFLPTSLDN 60
MV PSLFLLLLLNFH A VTELPL NL TYIVHVKKPE DDLEIWHRSFLP+S
Sbjct: 1 MVAFPSLFLLLLLNFHYVGALVTELPLINLQTYIVHVKKPETTDDLEIWHRSFLPSS--- 60
Query: 61 EEEQPTLLYSYRNVMSGFSARLTEEHVKAMEEKDGFVSARRETIVHLHTTHTPDFLGLNR 120
+LLYSYRNVMSGF+ARL+EE VKAMEE DGFVSARRE I+ LHTTHTPDFLGLNR
Sbjct: 61 ----SSLLYSYRNVMSGFAARLSEEQVKAMEEMDGFVSARRERILQLHTTHTPDFLGLNR 120
Query: 121 QFGFWKDSNFGKGVIIGVLDGGITPNHPSFDDAGMPQPPAKWKGRCEFNFSACNNKLIGA 180
QFGFWKDSNFGKGVIIGVLDGGI P+HPSFDD GMP PP KWKGRCEFNFSACNNKLIGA
Sbjct: 121 QFGFWKDSNFGKGVIIGVLDGGIAPSHPSFDDVGMPPPPPKWKGRCEFNFSACNNKLIGA 180
Query: 181 RSMNLASQALKGKITTLDDSPIDEDGHGTHTASTAAGTFVDGAEALGNAFGTAVGMAPLA 240
RS NLA++ALKG+ T +DDSPIDEDGHGTHTASTAAG FV GAEALGNA GTAVGMAPLA
Sbjct: 181 RSFNLATKALKGE-TMMDDSPIDEDGHGTHTASTAAGAFVKGAEALGNAKGTAVGMAPLA 240
Query: 241 HLAIYKVCFGEDCSDVDILAGLDAAVEDGVDVLSISLGGPSVPFFADITAIGSFAAIQKG 300
HLAIYKVCFGEDC D DILA LDAA+EDGVDVLS+SLG PSVPFF D+ AIG+FAAIQKG
Sbjct: 241 HLAIYKVCFGEDCPDTDILAALDAAIEDGVDVLSLSLGSPSVPFFQDLVAIGAFAAIQKG 300
Query: 301 IFVSCSAANSGPFNATLSNEAPWILTVAASTIDRKITATAKLGNGEEFDGESLFQPNDFP 360
IFVSCSAANSGPF ATLSNEAPWILTVAASTIDR+I A AKLGNGEEFDGESLFQP+DFP
Sbjct: 301 IFVSCSAANSGPFKATLSNEAPWILTVAASTIDRRIKAAAKLGNGEEFDGESLFQPSDFP 360
Query: 361 QTLLPLVFPGEKNETVALCAEGSLKNIDVKGKVVVCERGGGIARIAKGVEVKNGGGAAMI 420
TLLPLV+ GEKN+T ALC EGSLK+IDVKGKVVVCERGGGIARIAKG EVKN GGAAMI
Sbjct: 361 PTLLPLVYAGEKNQTAALCGEGSLKDIDVKGKVVVCERGGGIARIAKGTEVKNAGGAAMI 420
Query: 421 LLNAESDGFTTEVDAHVLPASHVSHTAALKIKAYINSTTYPTATILFKGTTIGDD-FSPA 480
LLN + DGF+TE DAHVLPASHVSH AALKIKAYINSTTYPTATILFKGT IGDD FSPA
Sbjct: 421 LLNQQQDGFSTEADAHVLPASHVSHKAALKIKAYINSTTYPTATILFKGTVIGDDNFSPA 480
Query: 481 IASFSSRGPSLASPGILKPDITGPGVSILAAWPFPLDNNTNTKSTFNIISGTSMSCPHLS 540
IASFSSRGPS+ASPGILKPDITGPGVSILAAWPFPLD N NTKSTFNIISGTSMSCPHLS
Sbjct: 481 IASFSSRGPSVASPGILKPDITGPGVSILAAWPFPLDKNRNTKSTFNIISGTSMSCPHLS 540
Query: 541 GIAALIKSAHPDWSPAAIKSSIMTTANITNLEGNPILDETLQPADLFAIGAGHVNPSKAI 600
GIAALIKS+HPDWSPAAIKS+IMTTA+ITNLEG PI+DE LQPADLFA GAGHVNPSKA
Sbjct: 541 GIAALIKSSHPDWSPAAIKSAIMTTADITNLEGQPIVDENLQPADLFATGAGHVNPSKAA 600
Query: 601 DPGLVYDIQPDDYIPYLCGLGYTNNQVSLIAHKPIDCLTTSSIPEGELNYPSFMVKLGPV 660
DPGLVYDIQPDDYIPYLCGLGY +N+V+ IA KPI+CL SIPEG+LNYPSF V LGP
Sbjct: 601 DPGLVYDIQPDDYIPYLCGLGYKSNEVATIARKPINCLAKPSIPEGDLNYPSFTVVLGPP 660
Query: 661 QTFSRTVTSVGSGRVVYNVVIEAPEGVSVTVRPRKLSFSALNQKATYSVTFKRIGSISPS 720
QTF+RTVT+VG GR VY V+EAP +SVT+RP K+ FS +NQK T+SVTFKRIGSISPS
Sbjct: 661 QTFTRTVTNVGCGREVYTAVVEAPPSISVTIRPSKIFFSKINQKVTFSVTFKRIGSISPS 720
Query: 721 IEFAEGYLKWVSAKHVVRSPISVKF 745
EF +GYLKWVS KHVVRSPIS KF
Sbjct: 721 TEFGKGYLKWVSDKHVVRSPISFKF 737
BLAST of Cmc09g0257051 vs. ExPASy Swiss-Prot
Match:
A9QY38 (Subtilisin-like protease 4 OS=Lotus japonicus OX=34305 GN=SBTM4 PE=2 SV=1)
HSP 1 Score: 976.9 bits (2524), Expect = 1.3e-283
Identity = 493/752 (65.56%), Postives = 592/752 (78.72%), Query Frame = 0
Query: 9 LLLLLNFHGYEAQVTELP--------LSNLHTYIVHVKKPE-----VVDDLEIWHRSFLP 68
L LL FH + AQ +ELP S+ YI+HV PE +DLE W+ SFLP
Sbjct: 9 LTFLLTFHVHNAQGSELPTTTTESTETSSSKIYIIHVTGPEGKMLTESEDLESWYHSFLP 68
Query: 69 TSLDNEEEQPTLLYSYRNVMSGFSARLTEEHVKAMEEKDGFVSARRETIVHLHTTHTPDF 128
+L + EEQP ++YSY+NV+ GF+A LT+E + A+E+K+GF+SA + ++H TTHTP F
Sbjct: 69 PTLMSSEEQPRVIYSYKNVLRGFAASLTQEELSAVEKKNGFISAHPQRVLHRQTTHTPKF 128
Query: 129 LGLNRQFGFWKDSNFGKGVIIGVLDGGITPNHPSFDDAGMPQPPAKWKGRCEFNFSACNN 188
LGL + G WK+SNFGKGVIIGVLD GITP HPSF D G+P PP KWKGRC+ N +ACNN
Sbjct: 129 LGLQQDTGVWKESNFGKGVIIGVLDSGITPGHPSFSDVGIPPPPPKWKGRCDLNVTACNN 188
Query: 189 KLIGARSMNLASQALKGKITTLDDSPIDEDGHGTHTASTAAGTFVDGAEALGNAFGTAVG 248
KLIGAR+ NLA++A+ GK ++PIDEDGHGTHTASTAAG FV+ AE LGNA GTA G
Sbjct: 189 KLIGARAFNLAAEAMNGKKA---EAPIDEDGHGTHTASTAAGAFVNYAEVLGNAKGTAAG 248
Query: 249 MAPLAHLAIYKVCFGEDCSDVDILAGLDAAVEDGVDVLSISLG-GPSVPFFADITAIGSF 308
MAP AHLAIYKVCFGEDC + DILA LDAAVEDGVDV+SISLG PFF D TAIG+F
Sbjct: 249 MAPHAHLAIYKVCFGEDCPESDILAALDAAVEDGVDVISISLGLSEPPPFFNDSTAIGAF 308
Query: 309 AAIQKGIFVSCSAANSGPFNATLSNEAPWILTVAASTIDRKITATAKLGNGEEFDGESLF 368
AA+QKGIFVSC+A NSGPFN+++ N APWILTV ASTIDR+I ATAKLGNG+EFDGES+F
Sbjct: 309 AAMQKGIFVSCAAGNSGPFNSSIVNAAPWILTVGASTIDRRIVATAKLGNGQEFDGESVF 368
Query: 369 QPNDFPQTLLPLVFPGEK-NETVALCAEGSLKNIDVKGKVVVCERGGGIARIAKGVEVKN 428
QP+ F TLLPL + G+ E A CA GSL + +GKVV+CERGGGIARIAKG EVK
Sbjct: 369 QPSSFTPTLLPLAYAGKNGKEESAFCANGSLDDSAFRGKVVLCERGGGIARIAKGEEVKR 428
Query: 429 GGGAAMILLNAESDGFTTEVDAHVLPASHVSHTAALKIKAYINSTTYPTATILFKGTTIG 488
GGAAMIL+N E++ F+ D H LPA+HVS+ A ++IKAYINST PTATILFKGT IG
Sbjct: 429 AGGAAMILMNDETNAFSLSADVHALPATHVSYAAGIEIKAYINSTATPTATILFKGTVIG 488
Query: 489 DDFSPAIASFSSRGPSLASPGILKPDITGPGVSILAAWPFPLDNNTNTKSTFNIISGTSM 548
+ +PA+ASFSSRGP+L SPGILKPDI GPGV+ILAAWPFPL N+T++K TFNI SGTSM
Sbjct: 489 NSLAPAVASFSSRGPNLPSPGILKPDIIGPGVNILAAWPFPLSNSTDSKLTFNIESGTSM 548
Query: 549 SCPHLSGIAALIKSAHPDWSPAAIKSSIMTTANITNLEGNPILDETLQPADLFAIGAGHV 608
SCPHLSGIAAL+KS+HP WSPAAIKS+IMT+A+ NL I+DETLQP DLFA G+GHV
Sbjct: 549 SCPHLSGIAALLKSSHPHWSPAAIKSAIMTSADTINLGNKLIVDETLQPTDLFATGSGHV 608
Query: 609 NPSKAIDPGLVYDIQPDDYIPYLCGLGYTNNQVSLIAHKPIDCLTTSSIPEGELNYPSFM 668
NPS+A DPGLVYDIQPDDYIPYLCGLGY+ +V +IAH+ I C ++SIPEGELNYPSF
Sbjct: 609 NPSRANDPGLVYDIQPDDYIPYLCGLGYSETEVGIIAHRKIKC--SASIPEGELNYPSFS 668
Query: 669 VKLGPVQTFSRTVTSVGSGRVVYNVVIEAPEGVSVTVRPRKLSFSALNQKATYSVTFKRI 728
V+LG +TF+RTVT+VG Y++++ AP+GV V V+P KL+FS +NQK TYSVTF R
Sbjct: 669 VELGSSKTFTRTVTNVGEAHSSYDLIVAAPQGVDVKVQPYKLNFSEVNQKETYSVTFSRT 728
Query: 729 GSISPSIEFAEGYLKWVSAKHVVRSPISVKFV 746
G + + E+A+G+LKWVS KH VRSPISVKF+
Sbjct: 729 GLGNKTQEYAQGFLKWVSTKHTVRSPISVKFI 755
BLAST of Cmc09g0257051 vs. ExPASy Swiss-Prot
Match:
A0A0M3R8G2 (Subtilisin-like protease OS=Petunia hybrida OX=4102 GN=SBT PE=2 SV=1)
HSP 1 Score: 865.9 bits (2236), Expect = 3.3e-250
Identity = 451/742 (60.78%), Postives = 545/742 (73.45%), Query Frame = 0
Query: 9 LLLLLNFHGYEAQVTELPLSNLHTYIVHVKKPEV-----VDDLEIWHRSFLPTSLDNEEE 68
+ LL+FH AQ + + L TYIVHV KP+ DLE ++ SFLP ++ E
Sbjct: 9 IAFLLSFHLTTAQRS----TGLQTYIVHVDKPDAQVLANSADLESYYNSFLPATVSGSEV 68
Query: 69 QPTLLYSYRNVMSGFSARLTEEHVKAMEEKDGFVSARRETIVHLHTTHTPDFLGLNRQFG 128
+++SY +V +GF+A+L+ E VK ME+K GFVSA+ E ++ LHTTHTP+FLGL + G
Sbjct: 69 PSRIIHSYHHVATGFAAKLSGEEVKEMEKKAGFVSAKLEKVLTLHTTHTPNFLGLYQNMG 128
Query: 129 FWKDSNFGKGVIIGVLDGGITPNHPSFDDAGMPQPPAKWKGRCEFNFSA-CNNKLIGARS 188
FW++SN+GKGVIIG+LD GITP HPSF D MP PPAKWKG+CEF +A CN K+IGAR+
Sbjct: 129 FWQESNYGKGVIIGLLDTGITPGHPSFSDVNMPSPPAKWKGKCEFTGNATCNKKIIGARN 188
Query: 189 MNLASQALKGKITTLDDSPIDEDGHGTHTASTAAGTFVDGAEALGNAFGTAVGMAPLAHL 248
I+ P DE+GHGTHTASTAAG FV+ A GNA GTAVGMAPLAH+
Sbjct: 189 F----------ISGSGVPPTDEEGHGTHTASTAAGNFVNDANVFGNANGTAVGMAPLAHI 248
Query: 249 AIYKVCFGEDCSDVDILAGLDAAVEDGVDVLSISLGGPSVPFFADITAIGSFAAIQKGIF 308
A+YKVC + CSD DILA LDAA++DGVDVLS+SLGG S PF+ D AIG+FAAI+KGIF
Sbjct: 249 AMYKVCSEDGCSDADILAALDAAIDDGVDVLSLSLGGYSDPFYYDNIAIGAFAAIRKGIF 308
Query: 309 VSCSAANSGPFNATLSNEAPWILTVAASTIDRKITATAKLGNGEEFDGESLFQPNDFPQT 368
VS SA N GP N+TLSNEAPWILTV AST DRKI ATA LGNG+++DGES FQP DFP T
Sbjct: 309 VSASAGNDGPLNSTLSNEAPWILTVGASTHDRKIVATAVLGNGQQYDGESAFQPADFPHT 368
Query: 369 LLPLVFPGEKNETVALCAEGSLKNIDVKGKVVVCERGGGIARIAKGVEVKNGGGAAMILL 428
LLPLV+PG +E A C+ GSL DVKGKVVVC+RGG +AR+ K VK+ GGAAMIL
Sbjct: 369 LLPLVYPGTSDEEAAFCSSGSLDKFDVKGKVVVCDRGGDVARLEKSQTVKDAGGAAMILA 428
Query: 429 NAESDGFTTEVDAHVLPASHVSHTAALKIKAYINSTTYPTATILFKGTTIGDDFSPAIAS 488
N E DG T DAHVLPA+HV + A IK+YINST+ PTA ILFKGT IG SP+++S
Sbjct: 429 NLEIDGEGTFADAHVLPATHVGYAAGEMIKSYINSTSTPTAGILFKGTIIGFKSSPSVSS 488
Query: 489 FSSRGPSLASPGILKPDITGPGVSILAAWPFPLDNNTNTKSTFNIISGTSMSCPHLSGIA 548
FSSRGP+LASPGI+KPDI GPGV+ILAAWP ++N T T TFNIISGTSMSCPHLSGI
Sbjct: 489 FSSRGPNLASPGIVKPDIIGPGVNILAAWPVSVENKTGTDLTFNIISGTSMSCPHLSGIV 548
Query: 549 ALIKSAHPDWSPAAIKSSIMTTANITNLEGNPILDETLQPADLFAIGAGHVNPSKAIDPG 608
AL+KSAHPDWSPAAIKS+IMT+A+ +NLEG PILDE PAD+FA GAGHVNPSKA DPG
Sbjct: 549 ALLKSAHPDWSPAAIKSAIMTSADQSNLEGQPILDERNLPADIFATGAGHVNPSKASDPG 608
Query: 609 LVYDIQPDDYIPYLCGLGYTNNQVSLIAHKPIDCLTTSSIPEGELNYPSFMVKLGP-VQT 668
L+YDIQ +DYI YLCGLGY + LI + + C SSI E ELNYPSF + LGP Q
Sbjct: 609 LIYDIQLEDYIQYLCGLGYREKDIGLIVQETVKC--ESSISEAELNYPSFSIILGPKTQN 668
Query: 669 FSRTVTSVGSGRVVYNVVIEAPEGVSVTVRPRKLSFSALNQKATYSVTFKRIGSISPSIE 728
++RTVT+VG Y V I +GV + V P L F+ + Q+ATY+V+F + G +
Sbjct: 669 YTRTVTNVGDASSTYTVNIAKTQGVDIVVEPATLVFTKMYQQATYTVSFTQSGDFTD--R 728
Query: 729 FAEGYLKWVSAKHVVRSPISVK 744
F +G + W S ++VVRSPISVK
Sbjct: 729 FVQGAISWSSNEYVVRSPISVK 732
BLAST of Cmc09g0257051 vs. ExPASy Swiss-Prot
Match:
A9QY40 (Subtilisin-like protease 1 OS=Lotus japonicus OX=34305 GN=SBTM1 PE=2 SV=1)
HSP 1 Score: 835.5 bits (2157), Expect = 4.7e-241
Identity = 442/729 (60.63%), Postives = 536/729 (73.53%), Query Frame = 0
Query: 29 NLHTYIVHVKKPEV------VDDLEIWHRSFLPTSLDNEEEQPTLLYSYRNVMSGFSARL 88
NL TYIVHVKK E+ ++L WH SFLP + + + +++SYRNV SGF+ RL
Sbjct: 37 NLTTYIVHVKKLEIEGPLQSTEELHTWHHSFLPETSNKDR----MVFSYRNVASGFAVRL 96
Query: 89 TEEHVKAMEEKDGFVSARRETIVHLHTTHTPDFLGLNRQFGFWKDSNFGKGVIIGVLDGG 148
T E A++EK+ +S R E + LHTTHTP FLGL + G W DSN GKGVIIGV+D G
Sbjct: 97 TPEEANALQEKEEVMSIRPERTLSLHTTHTPSFLGLRQGQGLWNDSNLGKGVIIGVIDTG 156
Query: 149 ITPNHPSFDDAGMPQPPAKWKGRCEF-NFSACNNKLIGARSMNLASQALKGKITTLDDSP 208
I P H SF+D GMP PPAKWKG CEF S CNNKLIGAR NL A++ + P
Sbjct: 157 IYPFHLSFNDEGMPPPPAKWKGHCEFTGGSVCNNKLIGAR--NLVKSAIQ-------EPP 216
Query: 209 IDEDGHGTHTASTAAGTFVDGAEALGNAFGTAVGMAPLAHLAIYKVCFG---EDCSDVDI 268
++ HGTHTA+ AAG FV+GA GNA GTA GMAP AHLAIYKVC ++C + I
Sbjct: 217 YEDFFHGTHTAAEAAGRFVEGASVFGNARGTAAGMAPDAHLAIYKVCSSKVKDECPESAI 276
Query: 269 LAGLDAAVEDGVDVLSISLGGPSVPFFADITAIGSFAAIQKGIFVSCSAANSGPFNATLS 328
LA +D A+EDGVDVLS+SLG S+PFF D AIG+FAA QKGIFVSCSAANSGP ++LS
Sbjct: 277 LAAMDIAIEDGVDVLSLSLGLGSLPFFEDPIAIGAFAATQKGIFVSCSAANSGPHYSSLS 336
Query: 329 NEAPWILTVAASTIDRKITATAKLGNGEEFDGESLFQPNDFPQTLLPLVF-PGEKNETVA 388
NEAPWILTV ASTIDRKI+A+AKLGNG E++GE+LFQP DF LLPLV+ EKN + A
Sbjct: 337 NEAPWILTVGASTIDRKISASAKLGNGAEYEGETLFQPKDFSSQLLPLVYAAAEKNNSSA 396
Query: 389 LCAEGSLKNIDVKGKVVVCERGGGIARIAKGVEVKNGGGAAMILLNAESDGFTTEVDAHV 448
LCA GSL+NI+VKGKVVVC+ GGGI IAKG EV + GG+AMIL N E+ GFTT +AHV
Sbjct: 397 LCAPGSLRNINVKGKVVVCDLGGGIPFIAKGQEVLDAGGSAMILANIENFGFTTLANAHV 456
Query: 449 LPASHVSHTAALKIKAYINSTTYPTATILFKGTTIGDDFSPAIASFSSRGPSLASPGILK 508
LPA HVS+ A+L IKAYINST PTAT+LF+GT IGD +P++A+FSSRGPS SPGILK
Sbjct: 457 LPAVHVSYAASLAIKAYINSTYTPTATVLFQGTIIGDSLAPSVAAFSSRGPSQQSPGILK 516
Query: 509 PDITGPGVSILAAWPFPLDNNTNTKSTFNIISGTSMSCPHLSGIAALIKSAHPDWSPAAI 568
PDI GPGV+ILAAW +DN F+IISGTSMSCPHLSGIAAL+KSAHPDWSPAAI
Sbjct: 517 PDIIGPGVNILAAWAVSVDNKI---PAFDIISGTSMSCPHLSGIAALLKSAHPDWSPAAI 576
Query: 569 KSSIMTTANITNLEGNPILDETLQPADLFAIGAGHVNPSKAIDPGLVYDIQPDDYIPYLC 628
KS+IMTTAN NL G PILD+ LQPAD+FA GAGHVNP +A DPGLVYDIQP+DY+PYLC
Sbjct: 577 KSAIMTTANTLNLRGLPILDQRLQPADIFATGAGHVNPVRANDPGLVYDIQPEDYVPYLC 636
Query: 629 GLGYTNNQVSLIAHKPIDCLTTSSIPEGELNYPSFMVKLG-PVQTFSRTVTSVGSGRVVY 688
GLGY++ +V++I + + C SI + ELNYPSF + LG Q ++RT+T+VG Y
Sbjct: 637 GLGYSDREVTIIVQRSVRCFNVKSIAQAELNYPSFSILLGSDSQFYTRTLTNVGPANSTY 696
Query: 689 NVVIEAPEGVSVTVRPRKLSFSALNQKATYSVTF-KRIGSISPSIEFAEGYLKWVSAKHV 745
V I+ P + ++V P +++F+ +NQK Y V F +I + FA+G + WVS KHV
Sbjct: 697 TVKIDVPLAMGISVSPSQITFTQVNQKVAYFVDFIPQIKENRGNHTFAQGAITWVSDKHV 749
BLAST of Cmc09g0257051 vs. ExPASy Swiss-Prot
Match:
A9QY39 (Subtilisin-like protease 3 OS=Lotus japonicus OX=34305 GN=SBTM3 PE=2 SV=1)
HSP 1 Score: 814.7 bits (2103), Expect = 8.7e-235
Identity = 440/755 (58.28%), Postives = 539/755 (71.39%), Query Frame = 0
Query: 3 LLPSLFLLLLLNFHGYEAQ---VTEL---PLSNLHTYIVHVKKPEVV--DDLEIWHRSFL 62
LL +L L+L ++ +Q TEL SNL TYIVHV+KP+V+ DDL ++ S L
Sbjct: 9 LLLALILVLSVSLASAASQNLEFTELEDEDQSNLSTYIVHVRKPQVIQSDDLHTFYYSLL 68
Query: 63 PTSLDNEEEQPTLLYSYRNVMSGFSARLTEEHVKAMEEKDGFVSARRETIVHLHTTHTPD 122
P S ++ ++++YRNV++GF+ +LT E KA+++ + VSAR E I+ LHTTHTP
Sbjct: 69 PESTKTTNQR--IVFTYRNVVNGFAVKLTPEEAKALQQNEEVVSARPEKILSLHTTHTPS 128
Query: 123 FLGLNRQFGFWKDSNFGKGVIIGVLDGGITPNHPSFDDAGMPQPPAKWKGRCEFNFS-AC 182
FLGL + G WK SN GKGVIIG+LD GI+P HPSF D GMP PPAKW G CEF C
Sbjct: 129 FLGLQQGLGLWKGSNSGKGVIIGILDTGISPFHPSFSDEGMPSPPAKWNGICEFTGKRTC 188
Query: 183 NNKLIGARSMNLASQALKGKITTLDDSPIDEDGHGTHTASTAAGTFVDGAEALGNAFGTA 242
NNK+IGAR+ +K K TL P D+ GHGTHTASTAAG V GA GNA GTA
Sbjct: 189 NNKIIGARNF------VKTKNLTL---PFDDVGHGTHTASTAAGRPVQGANVYGNANGTA 248
Query: 243 VGMAPLAHLAIYKVCFGEDCSDVDILAGLDAAVEDGVDVLSISLGGPSVPFFADITAIGS 302
VGMAP AH+A+YKVC CS+ ILAG+D AV+DGVDVLS+SLGGPS PFF D A+G+
Sbjct: 249 VGMAPDAHIAMYKVCGLVGCSESAILAGMDTAVDDGVDVLSLSLGGPSGPFFEDPIALGA 308
Query: 303 FAAIQKGIFVSCSAANSGPFNATLSNEAPWILTVAASTIDRKITATAKLGNGEEFDGESL 362
F AIQKGIFVSCSAANSGP ++LSNEAPWILTV AS+IDR I ATAKLGNG+E+ G+S+
Sbjct: 309 FGAIQKGIFVSCSAANSGPAYSSLSNEAPWILTVGASSIDRTIMATAKLGNGKEYVGQSV 368
Query: 363 FQPNDFPQTLLPLVFPGE--KNETVALCAEGSLKNIDVKGKVVVCERGGGIARIAKGVEV 422
FQP DF +LLPLV+ G N CA SL DV+GKVV+CE GG + R+ KG V
Sbjct: 369 FQPKDFAPSLLPLVYAGANGNNNFSVFCAPESLNRSDVEGKVVLCEDGGFVPRVFKGKAV 428
Query: 423 KNGGGAAMILLNAESDGFTTEVDAHVLPASHVSHTAALKIKAYINSTTYPTATILFKGTT 482
K+ GGAAMIL+N+ + F D HVLPA H+S+ A L +K YINST+ PTATILF+GT
Sbjct: 429 KDAGGAAMILMNSVLEDFNPIADVHVLPAVHISYEAGLALKEYINSTSTPTATILFEGTV 488
Query: 483 IGDDFSPAIASFSSRGPSLASPGILKPDITGPGVSILAAWPFPLDNNTNTKSTFNIISGT 542
IG+ +P + SFSSRGPS ASPGILKPDI GPG++ILAAWP LDN+T FNIISGT
Sbjct: 489 IGNLLAPQVTSFSSRGPSKASPGILKPDIIGPGLNILAAWPVSLDNSTT--PPFNIISGT 548
Query: 543 SMSCPHLSGIAALIKSAHPDWSPAAIKSSIMTTANITNLEGNPILDETLQPADLFAIGAG 602
SMSCPHLSGIAAL+K++HPDWSPAAIKS+IMTTA+ NL G PILD+ L PAD+FA GAG
Sbjct: 549 SMSCPHLSGIAALLKNSHPDWSPAAIKSAIMTTASQVNLGGTPILDQRLVPADVFATGAG 608
Query: 603 HVNPSKAIDPGLVYDIQPDDYIPYLCGLGYTNNQVSLIAHKPIDCLTTSSIPEGELNYPS 662
HVNP KA DPGLVYDI+P+DYIPYLCGL YT+ +V +I + + C + I E ELNYPS
Sbjct: 609 HVNPVKANDPGLVYDIEPNDYIPYLCGLNYTDREVGVILQQRVRCSEVNHIAEAELNYPS 668
Query: 663 FMVKLG-PVQTFSRTVTSVGSGRVVYNVVIEAPEGVSVTVRPRKLSFSALNQKATYSVTF 722
F + LG Q ++RTV +VG Y I P GV +++ P +L+F+ + QK TYSV+F
Sbjct: 669 FSILLGNTTQLYTRTVANVGPANSTYTAEIGVPVGVGMSLSPAQLTFTEVGQKLTYSVSF 728
Query: 723 KRIGSISPSIEFAEGYLKWVSAKHVVRSPISVKFV 746
+ FA+G LKWVS K+ VRSPIS F+
Sbjct: 729 IPFSEDRDNHTFAQGSLKWVSGKYSVRSPISFIFL 750
BLAST of Cmc09g0257051 vs. ExPASy Swiss-Prot
Match:
G7KEU7 (Subtilisin-like protease OS=Medicago truncatula OX=3880 GN=SBT PE=2 SV=1)
HSP 1 Score: 794.3 bits (2050), Expect = 1.2e-228
Identity = 428/757 (56.54%), Postives = 532/757 (70.28%), Query Frame = 0
Query: 2 VLLPSLFLLLLLNFHGYEAQVTELPLSNLHTYIVHVKKPEVV-----DDLEIWHRSFLPT 61
+L+ +F+L ++ E + E NL TYIVHVKK E V +DL W+ SFLP
Sbjct: 8 LLVSLIFILCSISMLAAEENL-EHDQINLMTYIVHVKKSENVASHQSEDLHSWYHSFLPQ 67
Query: 62 SLDNEEEQPTLLYSYRNVMSGFSARLTEEHVKAMEEKDGFVSARRETIVHLHTTHTPDFL 121
+ ++E +++SYR V SGF+ +LT E K+++EK VSAR E + LHTTHTP FL
Sbjct: 68 TFPHKER---MVFSYRKVASGFAVKLTPEEAKSLQEKGEIVSARPERTLELHTTHTPTFL 127
Query: 122 GLNRQFGFWKDSNFGKGVIIGVLDGGITPNHPSFDDAGMPQPPAKWKGRCEF-NFSACNN 181
GL + G W D N GKGVIIG++D GI P HPSF+D GMP PPAKWKG CEF CNN
Sbjct: 128 GLKQGQGLWSDDNLGKGVIIGIIDTGIFPLHPSFNDEGMPPPPAKWKGHCEFTGGQVCNN 187
Query: 182 KLIGARSMNLASQALKGKITTLDDSPIDEDGHGTHTASTAAGTFVDGAEALGNAFGTAVG 241
KLIGAR NL A++ + P + HGTHTA+ AAG F++ A GNA G A G
Sbjct: 188 KLIGAR--NLVKSAIQ-------EPPFENFFHGTHTAAEAAGRFIEDASVFGNAKGVAAG 247
Query: 242 MAPLAHLAIYKVCFGE-DCSDVDILAGLDAAVEDGVDVLSISLGGPSVPFFADITAIGSF 301
MAP AHLAIYKVC + C++ ILA +D A+EDGVDVLS+SLG S+PFF D AIG+F
Sbjct: 248 MAPNAHLAIYKVCNDKIGCTESAILAAMDIAIEDGVDVLSLSLGLGSLPFFEDPIAIGAF 307
Query: 302 AAIQKGIFVSCSAANSGPFNATLSNEAPWILTVAASTIDRKITATAKLGNGEEFDGESLF 361
AA Q G+FVSCSAANSGP +TLSNEAPWILTV ASTIDRKI A+AKLGNGEE++GE+LF
Sbjct: 308 AATQNGVFVSCSAANSGPGYSTLSNEAPWILTVGASTIDRKIVASAKLGNGEEYEGETLF 367
Query: 362 QPNDFPQTLLPLVFP-----GEKNETVALCAEGSLKNIDVKGKVVVCERGGGIARIAKGV 421
QP DF Q LLPLV+P G + + +LC GSLKNID+ GKVV+C+ G ++ I KG
Sbjct: 368 QPKDFSQQLLPLVYPGSFGYGNQTQNQSLCLPGSLKNIDLSGKVVLCD-VGNVSSIVKGQ 427
Query: 422 EVKNGGGAAMILLNAESDGFTTEVDAHVLPASHVSHTAALKIKAYINSTTYPTATILFKG 481
EV N GG AMIL N+E+ GF+T AHVLPA VS+ A L IK+YI ST PTAT++FKG
Sbjct: 428 EVLNSGGIAMILANSEALGFSTFAIAHVLPAVEVSYAAGLTIKSYIKSTYNPTATLIFKG 487
Query: 482 TTIGDDFSPAIASFSSRGPSLASPGILKPDITGPGVSILAAWPFPLDNNTNTKSTFNIIS 541
T IGD +P++ FSSRGPS SPGILKPDI GPGV+ILAAW +DN F+I+S
Sbjct: 488 TIIGDSLAPSVVYFSSRGPSQESPGILKPDIIGPGVNILAAWAVSVDNKI---PAFDIVS 547
Query: 542 GTSMSCPHLSGIAALIKSAHPDWSPAAIKSSIMTTANITNLEGNPILDETLQPADLFAIG 601
GTSMSCPHLSGIAALIKS+HPDWSPAAIKS+IMTTAN NL G PILD+ L PAD+FA G
Sbjct: 548 GTSMSCPHLSGIAALIKSSHPDWSPAAIKSAIMTTANTLNLGGIPILDQRLFPADIFATG 607
Query: 602 AGHVNPSKAIDPGLVYDIQPDDYIPYLCGLGYTNNQVSLIAHKPIDCLTTSSIPEGELNY 661
AGHVNP KA DPGLVYDI+P+DY+PYLCGLGY++ ++ +I + C SIPE +LNY
Sbjct: 608 AGHVNPVKANDPGLVYDIEPEDYVPYLCGLGYSDKEIEVIVQWKVKCSNVKSIPEAQLNY 667
Query: 662 PSFMVKLG-PVQTFSRTVTSVGSGRVVYNVVIEAPEGVSVTVRPRKLSFSALNQKATYSV 721
PSF + LG Q ++RT+T+VG Y V +E P + ++V P +++F+ +N+K ++SV
Sbjct: 668 PSFSILLGSDSQYYTRTLTNVGFANSTYKVELEVPLALGMSVNPSEITFTEVNEKVSFSV 727
Query: 722 TF-KRIGSISPSIEFAEGYLKWVSAKHVVRSPISVKF 745
F +I + F +G L WVS +H VR PISV F
Sbjct: 728 EFIPQIKENRRNHTFGQGSLTWVSDRHAVRIPISVIF 747
BLAST of Cmc09g0257051 vs. ExPASy TrEMBL
Match:
A0A5A7TJ55 (Subtilisin-like protease SBT1.7 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold44G003250 PE=3 SV=1)
HSP 1 Score: 1478.8 bits (3827), Expect = 0.0e+00
Identity = 745/745 (100.00%), Postives = 745/745 (100.00%), Query Frame = 0
Query: 1 MVLLPSLFLLLLLNFHGYEAQVTELPLSNLHTYIVHVKKPEVVDDLEIWHRSFLPTSLDN 60
MVLLPSLFLLLLLNFHGYEAQVTELPLSNLHTYIVHVKKPEVVDDLEIWHRSFLPTSLDN
Sbjct: 1 MVLLPSLFLLLLLNFHGYEAQVTELPLSNLHTYIVHVKKPEVVDDLEIWHRSFLPTSLDN 60
Query: 61 EEEQPTLLYSYRNVMSGFSARLTEEHVKAMEEKDGFVSARRETIVHLHTTHTPDFLGLNR 120
EEEQPTLLYSYRNVMSGFSARLTEEHVKAMEEKDGFVSARRETIVHLHTTHTPDFLGLNR
Sbjct: 61 EEEQPTLLYSYRNVMSGFSARLTEEHVKAMEEKDGFVSARRETIVHLHTTHTPDFLGLNR 120
Query: 121 QFGFWKDSNFGKGVIIGVLDGGITPNHPSFDDAGMPQPPAKWKGRCEFNFSACNNKLIGA 180
QFGFWKDSNFGKGVIIGVLDGGITPNHPSFDDAGMPQPPAKWKGRCEFNFSACNNKLIGA
Sbjct: 121 QFGFWKDSNFGKGVIIGVLDGGITPNHPSFDDAGMPQPPAKWKGRCEFNFSACNNKLIGA 180
Query: 181 RSMNLASQALKGKITTLDDSPIDEDGHGTHTASTAAGTFVDGAEALGNAFGTAVGMAPLA 240
RSMNLASQALKGKITTLDDSPIDEDGHGTHTASTAAGTFVDGAEALGNAFGTAVGMAPLA
Sbjct: 181 RSMNLASQALKGKITTLDDSPIDEDGHGTHTASTAAGTFVDGAEALGNAFGTAVGMAPLA 240
Query: 241 HLAIYKVCFGEDCSDVDILAGLDAAVEDGVDVLSISLGGPSVPFFADITAIGSFAAIQKG 300
HLAIYKVCFGEDCSDVDILAGLDAAVEDGVDVLSISLGGPSVPFFADITAIGSFAAIQKG
Sbjct: 241 HLAIYKVCFGEDCSDVDILAGLDAAVEDGVDVLSISLGGPSVPFFADITAIGSFAAIQKG 300
Query: 301 IFVSCSAANSGPFNATLSNEAPWILTVAASTIDRKITATAKLGNGEEFDGESLFQPNDFP 360
IFVSCSAANSGPFNATLSNEAPWILTVAASTIDRKITATAKLGNGEEFDGESLFQPNDFP
Sbjct: 301 IFVSCSAANSGPFNATLSNEAPWILTVAASTIDRKITATAKLGNGEEFDGESLFQPNDFP 360
Query: 361 QTLLPLVFPGEKNETVALCAEGSLKNIDVKGKVVVCERGGGIARIAKGVEVKNGGGAAMI 420
QTLLPLVFPGEKNETVALCAEGSLKNIDVKGKVVVCERGGGIARIAKGVEVKNGGGAAMI
Sbjct: 361 QTLLPLVFPGEKNETVALCAEGSLKNIDVKGKVVVCERGGGIARIAKGVEVKNGGGAAMI 420
Query: 421 LLNAESDGFTTEVDAHVLPASHVSHTAALKIKAYINSTTYPTATILFKGTTIGDDFSPAI 480
LLNAESDGFTTEVDAHVLPASHVSHTAALKIKAYINSTTYPTATILFKGTTIGDDFSPAI
Sbjct: 421 LLNAESDGFTTEVDAHVLPASHVSHTAALKIKAYINSTTYPTATILFKGTTIGDDFSPAI 480
Query: 481 ASFSSRGPSLASPGILKPDITGPGVSILAAWPFPLDNNTNTKSTFNIISGTSMSCPHLSG 540
ASFSSRGPSLASPGILKPDITGPGVSILAAWPFPLDNNTNTKSTFNIISGTSMSCPHLSG
Sbjct: 481 ASFSSRGPSLASPGILKPDITGPGVSILAAWPFPLDNNTNTKSTFNIISGTSMSCPHLSG 540
Query: 541 IAALIKSAHPDWSPAAIKSSIMTTANITNLEGNPILDETLQPADLFAIGAGHVNPSKAID 600
IAALIKSAHPDWSPAAIKSSIMTTANITNLEGNPILDETLQPADLFAIGAGHVNPSKAID
Sbjct: 541 IAALIKSAHPDWSPAAIKSSIMTTANITNLEGNPILDETLQPADLFAIGAGHVNPSKAID 600
Query: 601 PGLVYDIQPDDYIPYLCGLGYTNNQVSLIAHKPIDCLTTSSIPEGELNYPSFMVKLGPVQ 660
PGLVYDIQPDDYIPYLCGLGYTNNQVSLIAHKPIDCLTTSSIPEGELNYPSFMVKLGPVQ
Sbjct: 601 PGLVYDIQPDDYIPYLCGLGYTNNQVSLIAHKPIDCLTTSSIPEGELNYPSFMVKLGPVQ 660
Query: 661 TFSRTVTSVGSGRVVYNVVIEAPEGVSVTVRPRKLSFSALNQKATYSVTFKRIGSISPSI 720
TFSRTVTSVGSGRVVYNVVIEAPEGVSVTVRPRKLSFSALNQKATYSVTFKRIGSISPSI
Sbjct: 661 TFSRTVTSVGSGRVVYNVVIEAPEGVSVTVRPRKLSFSALNQKATYSVTFKRIGSISPSI 720
Query: 721 EFAEGYLKWVSAKHVVRSPISVKFV 746
EFAEGYLKWVSAKHVVRSPISVKFV
Sbjct: 721 EFAEGYLKWVSAKHVVRSPISVKFV 745
BLAST of Cmc09g0257051 vs. ExPASy TrEMBL
Match:
A0A1S3ATZ7 (subtilisin-like protease SBT1.7 OS=Cucumis melo OX=3656 GN=LOC103482686 PE=3 SV=1)
HSP 1 Score: 1473.4 bits (3813), Expect = 0.0e+00
Identity = 743/745 (99.73%), Postives = 743/745 (99.73%), Query Frame = 0
Query: 1 MVLLPSLFLLLLLNFHGYEAQVTELPLSNLHTYIVHVKKPEVVDDLEIWHRSFLPTSLDN 60
MVLLPSLFLLLLLNFHGYEAQVTELPLSNLHTYIVHVKKPEVVDDLEIWHRSFLPTSLDN
Sbjct: 1 MVLLPSLFLLLLLNFHGYEAQVTELPLSNLHTYIVHVKKPEVVDDLEIWHRSFLPTSLDN 60
Query: 61 EEEQPTLLYSYRNVMSGFSARLTEEHVKAMEEKDGFVSARRETIVHLHTTHTPDFLGLNR 120
EEEQPTLLYSYRNVMSGFSARLTEEHVKAMEEKDGFVSARRETIVHLHTTHTPDFLGLNR
Sbjct: 61 EEEQPTLLYSYRNVMSGFSARLTEEHVKAMEEKDGFVSARRETIVHLHTTHTPDFLGLNR 120
Query: 121 QFGFWKDSNFGKGVIIGVLDGGITPNHPSFDDAGMPQPPAKWKGRCEFNFSACNNKLIGA 180
QFGFWKDSNFGKGVIIGVLDGGITPNHPSFDDAGM QPPAKWKGRCEFNFSACNNKLIGA
Sbjct: 121 QFGFWKDSNFGKGVIIGVLDGGITPNHPSFDDAGMAQPPAKWKGRCEFNFSACNNKLIGA 180
Query: 181 RSMNLASQALKGKITTLDDSPIDEDGHGTHTASTAAGTFVDGAEALGNAFGTAVGMAPLA 240
RSMNLASQALKGKITTLDDSPIDEDGHGTHTASTAAGTFVDGAEALGNAFGTAVGMAPLA
Sbjct: 181 RSMNLASQALKGKITTLDDSPIDEDGHGTHTASTAAGTFVDGAEALGNAFGTAVGMAPLA 240
Query: 241 HLAIYKVCFGEDCSDVDILAGLDAAVEDGVDVLSISLGGPSVPFFADITAIGSFAAIQKG 300
HLAIYKVCFGEDCSDVDILAGLDAAVEDGVDVLSISLGGPSVPFFADITAIGSFAAIQKG
Sbjct: 241 HLAIYKVCFGEDCSDVDILAGLDAAVEDGVDVLSISLGGPSVPFFADITAIGSFAAIQKG 300
Query: 301 IFVSCSAANSGPFNATLSNEAPWILTVAASTIDRKITATAKLGNGEEFDGESLFQPNDFP 360
IFVSCSAANSGPFNATLSNEAPWILTVAASTIDRKITATAKLGNGEEFDGESLFQPNDFP
Sbjct: 301 IFVSCSAANSGPFNATLSNEAPWILTVAASTIDRKITATAKLGNGEEFDGESLFQPNDFP 360
Query: 361 QTLLPLVFPGEKNETVALCAEGSLKNIDVKGKVVVCERGGGIARIAKGVEVKNGGGAAMI 420
QTLLPLVFPGEKNETVALCAEGSLKNIDVKGKVVVCERGGGIARIAKGVEVKNGGGAAMI
Sbjct: 361 QTLLPLVFPGEKNETVALCAEGSLKNIDVKGKVVVCERGGGIARIAKGVEVKNGGGAAMI 420
Query: 421 LLNAESDGFTTEVDAHVLPASHVSHTAALKIKAYINSTTYPTATILFKGTTIGDDFSPAI 480
LLNAESDGFTTEVDAHVLPASHVSHTAALKIKAYINSTTYPTATILFKGTTIGDDFSPAI
Sbjct: 421 LLNAESDGFTTEVDAHVLPASHVSHTAALKIKAYINSTTYPTATILFKGTTIGDDFSPAI 480
Query: 481 ASFSSRGPSLASPGILKPDITGPGVSILAAWPFPLDNNTNTKSTFNIISGTSMSCPHLSG 540
ASFSSRGPSLASPGILKPDITGPGVSILAAWPFPLDNNTNTKSTFNIISGTSMSCPHLSG
Sbjct: 481 ASFSSRGPSLASPGILKPDITGPGVSILAAWPFPLDNNTNTKSTFNIISGTSMSCPHLSG 540
Query: 541 IAALIKSAHPDWSPAAIKSSIMTTANITNLEGNPILDETLQPADLFAIGAGHVNPSKAID 600
IAALIKSAHPDWSPAAIKSSIMTTANITNLEGNPILDETLQPADLFAIGAGHVNPSKAID
Sbjct: 541 IAALIKSAHPDWSPAAIKSSIMTTANITNLEGNPILDETLQPADLFAIGAGHVNPSKAID 600
Query: 601 PGLVYDIQPDDYIPYLCGLGYTNNQVSLIAHKPIDCLTTSSIPEGELNYPSFMVKLGPVQ 660
PGLVYDIQPDDYIPYLCGLGYTNNQVSLIAHKPIDCLTTSSIPEGELNYPSFMVKLGPVQ
Sbjct: 601 PGLVYDIQPDDYIPYLCGLGYTNNQVSLIAHKPIDCLTTSSIPEGELNYPSFMVKLGPVQ 660
Query: 661 TFSRTVTSVGSGRVVYNVVIEAPEGVSVTVRPRKLSFSALNQKATYSVTFKRIGSISPSI 720
TFSRTVTSVGSGRVVYNVVIEAPEGVSVTVRPRKLSFSALNQKATYSVTFKR GSISPSI
Sbjct: 661 TFSRTVTSVGSGRVVYNVVIEAPEGVSVTVRPRKLSFSALNQKATYSVTFKRSGSISPSI 720
Query: 721 EFAEGYLKWVSAKHVVRSPISVKFV 746
EFAEGYLKWVSAKHVVRSPISVKFV
Sbjct: 721 EFAEGYLKWVSAKHVVRSPISVKFV 745
BLAST of Cmc09g0257051 vs. ExPASy TrEMBL
Match:
A0A0A0KKE3 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G157240 PE=3 SV=1)
HSP 1 Score: 1423.3 bits (3683), Expect = 0.0e+00
Identity = 713/745 (95.70%), Postives = 730/745 (97.99%), Query Frame = 0
Query: 1 MVLLPSLFLLLLLNFHGYEAQVTELPLSNLHTYIVHVKKPEVVDDLEIWHRSFLPTSLDN 60
MVLLPSLFLLLLLNFH YEAQVTELP SNLHTYIVHVKKPEVVDDLE WHRSFLPTSL+N
Sbjct: 1 MVLLPSLFLLLLLNFHVYEAQVTELPFSNLHTYIVHVKKPEVVDDLESWHRSFLPTSLEN 60
Query: 61 EEEQPTLLYSYRNVMSGFSARLTEEHVKAMEEKDGFVSARRETIVHLHTTHTPDFLGLNR 120
EEQPTLLYSYRNVMSGFSARLTEEHVKAMEEKDGFVSARRETIVHLHTTH+P+FLGLNR
Sbjct: 61 SEEQPTLLYSYRNVMSGFSARLTEEHVKAMEEKDGFVSARRETIVHLHTTHSPNFLGLNR 120
Query: 121 QFGFWKDSNFGKGVIIGVLDGGITPNHPSFDDAGMPQPPAKWKGRCEFNFSACNNKLIGA 180
QFGFWKDSNFGKGVIIGVLDGGITP+HPSF DAGMPQPPAKWKGRCEFNFSACNNKLIGA
Sbjct: 121 QFGFWKDSNFGKGVIIGVLDGGITPSHPSFVDAGMPQPPAKWKGRCEFNFSACNNKLIGA 180
Query: 181 RSMNLASQALKGKITTLDDSPIDEDGHGTHTASTAAGTFVDGAEALGNAFGTAVGMAPLA 240
RS+NLASQALKGKITTLDDSPIDEDGHGTHTASTAAGTFVDGAEALGNAFGTAVGMAPLA
Sbjct: 181 RSLNLASQALKGKITTLDDSPIDEDGHGTHTASTAAGTFVDGAEALGNAFGTAVGMAPLA 240
Query: 241 HLAIYKVCFGEDCSDVDILAGLDAAVEDGVDVLSISLGGPSVPFFADITAIGSFAAIQKG 300
HLAIYKVCFGE CS+VDILAGLDAAVEDGVDVLSISLGGP VPFFADITAIG+FAAIQKG
Sbjct: 241 HLAIYKVCFGESCSNVDILAGLDAAVEDGVDVLSISLGGPPVPFFADITAIGAFAAIQKG 300
Query: 301 IFVSCSAANSGPFNATLSNEAPWILTVAASTIDRKITATAKLGNGEEFDGESLFQPNDFP 360
IFVSCSAANSGPFNATLSNEAPWILTVAASTIDRKITATAKLGNGEEFDGESLFQPNDFP
Sbjct: 301 IFVSCSAANSGPFNATLSNEAPWILTVAASTIDRKITATAKLGNGEEFDGESLFQPNDFP 360
Query: 361 QTLLPLVFPGEKNETVALCAEGSLKNIDVKGKVVVCERGGGIARIAKGVEVKNGGGAAMI 420
QT LPLVFPGEKNETVALCAEGSLKNIDVKGKVVVC+RGGGIARIAKGVEVKN GGAAMI
Sbjct: 361 QTFLPLVFPGEKNETVALCAEGSLKNIDVKGKVVVCDRGGGIARIAKGVEVKNAGGAAMI 420
Query: 421 LLNAESDGFTTEVDAHVLPASHVSHTAALKIKAYINSTTYPTATILFKGTTIGDDFSPAI 480
LLNAESDGFTTE DAHVLPASHVSHTAALKIKAYINSTTYPTATI+FKGTTIGDDFSPAI
Sbjct: 421 LLNAESDGFTTEADAHVLPASHVSHTAALKIKAYINSTTYPTATIVFKGTTIGDDFSPAI 480
Query: 481 ASFSSRGPSLASPGILKPDITGPGVSILAAWPFPLDNNTNTKSTFNIISGTSMSCPHLSG 540
A+FSSRGPSLASPGILKPDITGPGVSILAAWPFPLDNNTNTKSTFNI+SGTSMSCPHLSG
Sbjct: 481 AAFSSRGPSLASPGILKPDITGPGVSILAAWPFPLDNNTNTKSTFNIVSGTSMSCPHLSG 540
Query: 541 IAALIKSAHPDWSPAAIKSSIMTTANITNLEGNPILDETLQPADLFAIGAGHVNPSKAID 600
IAALIKSAHPDWSPAAIKSSIMTTANITNLEGNPI+D+TLQPADLFAIGAGHVNPSKA+D
Sbjct: 541 IAALIKSAHPDWSPAAIKSSIMTTANITNLEGNPIVDQTLQPADLFAIGAGHVNPSKAVD 600
Query: 601 PGLVYDIQPDDYIPYLCGLGYTNNQVSLIAHKPIDCLTTSSIPEGELNYPSFMVKLGPVQ 660
PGLVYDIQPDDYIPYLCGLGYTNNQVSLIAHKPIDCLTT+SIPEGELNYPSFMVKLG VQ
Sbjct: 601 PGLVYDIQPDDYIPYLCGLGYTNNQVSLIAHKPIDCLTTTSIPEGELNYPSFMVKLGQVQ 660
Query: 661 TFSRTVTSVGSGRVVYNVVIEAPEGVSVTVRPRKLSFSALNQKATYSVTFKRIGSISPSI 720
TFSRTVT VGSGR VYNVVIEAPEGVSVTVRPRK+ FSALNQKATYSVTFKRIGSISPS
Sbjct: 661 TFSRTVTYVGSGREVYNVVIEAPEGVSVTVRPRKVIFSALNQKATYSVTFKRIGSISPST 720
Query: 721 EFAEGYLKWVSAKHVVRSPISVKFV 746
EFAEGYLKWVSAKH+VRSPISVKFV
Sbjct: 721 EFAEGYLKWVSAKHLVRSPISVKFV 745
BLAST of Cmc09g0257051 vs. ExPASy TrEMBL
Match:
A0A6J1H242 (subtilisin-like protease SBT1.8 OS=Cucurbita moschata OX=3662 GN=LOC111459745 PE=3 SV=1)
HSP 1 Score: 1236.9 bits (3199), Expect = 0.0e+00
Identity = 623/745 (83.62%), Postives = 663/745 (88.99%), Query Frame = 0
Query: 1 MVLLPSLFLLLLLNFHGYEAQVTELPLSNLHTYIVHVKKPEVVDDLEIWHRSFLPTSLDN 60
MV PSLFLLLLLNFH A VTELPL NL TYIVHVKKPE DDLEIWHRSFLP+S
Sbjct: 1 MVAFPSLFLLLLLNFHYVGALVTELPLINLQTYIVHVKKPETTDDLEIWHRSFLPSS--- 60
Query: 61 EEEQPTLLYSYRNVMSGFSARLTEEHVKAMEEKDGFVSARRETIVHLHTTHTPDFLGLNR 120
+LLYSYRNVMSGF+ARL+EE VKAMEE DGFVSARRE I+ LHTTHTPDFLGLNR
Sbjct: 61 ----SSLLYSYRNVMSGFAARLSEEQVKAMEEMDGFVSARRERILQLHTTHTPDFLGLNR 120
Query: 121 QFGFWKDSNFGKGVIIGVLDGGITPNHPSFDDAGMPQPPAKWKGRCEFNFSACNNKLIGA 180
QFGFWKDSNFGKGVIIGVLDGGI P+HPSFDD GMP PP KWKGRCEFNFSACNNKLIGA
Sbjct: 121 QFGFWKDSNFGKGVIIGVLDGGIAPSHPSFDDVGMPPPPPKWKGRCEFNFSACNNKLIGA 180
Query: 181 RSMNLASQALKGKITTLDDSPIDEDGHGTHTASTAAGTFVDGAEALGNAFGTAVGMAPLA 240
RS NLA++ALKG+ T +DDSPIDEDGHGTHTASTAAG FV GAEALGNA GTAVGMAPLA
Sbjct: 181 RSFNLATKALKGE-TMMDDSPIDEDGHGTHTASTAAGAFVKGAEALGNAKGTAVGMAPLA 240
Query: 241 HLAIYKVCFGEDCSDVDILAGLDAAVEDGVDVLSISLGGPSVPFFADITAIGSFAAIQKG 300
HLAIYKVCFGEDC D DILA LDAA+EDGVDVLS+SLG PSVPFF D+ AIG+FAAIQKG
Sbjct: 241 HLAIYKVCFGEDCPDTDILAALDAAIEDGVDVLSLSLGSPSVPFFQDLVAIGAFAAIQKG 300
Query: 301 IFVSCSAANSGPFNATLSNEAPWILTVAASTIDRKITATAKLGNGEEFDGESLFQPNDFP 360
IFVSCSAANSGPF ATLSNEAPWILTVAASTIDR+I A AKLGNGEEFDGESLFQP+DFP
Sbjct: 301 IFVSCSAANSGPFKATLSNEAPWILTVAASTIDRRIKAAAKLGNGEEFDGESLFQPSDFP 360
Query: 361 QTLLPLVFPGEKNETVALCAEGSLKNIDVKGKVVVCERGGGIARIAKGVEVKNGGGAAMI 420
TLLPLV+ GEKN+T ALC EGSLK+IDVKGKVVVCERGGGIARIAKG EVKN GGAAMI
Sbjct: 361 PTLLPLVYAGEKNQTAALCGEGSLKDIDVKGKVVVCERGGGIARIAKGTEVKNAGGAAMI 420
Query: 421 LLNAESDGFTTEVDAHVLPASHVSHTAALKIKAYINSTTYPTATILFKGTTIGDD-FSPA 480
LLN + DGF+TE DAHVLPASHVSH AALKIKAYINSTTYPTATILFKGT IGDD FSPA
Sbjct: 421 LLNQQQDGFSTEADAHVLPASHVSHKAALKIKAYINSTTYPTATILFKGTVIGDDNFSPA 480
Query: 481 IASFSSRGPSLASPGILKPDITGPGVSILAAWPFPLDNNTNTKSTFNIISGTSMSCPHLS 540
IASFSSRGPS+ASPGILKPDITGPGVSILAAWPFPLD N NTKSTFNIISGTSMSCPHLS
Sbjct: 481 IASFSSRGPSVASPGILKPDITGPGVSILAAWPFPLDKNRNTKSTFNIISGTSMSCPHLS 540
Query: 541 GIAALIKSAHPDWSPAAIKSSIMTTANITNLEGNPILDETLQPADLFAIGAGHVNPSKAI 600
GIAALIKS+HPDWSPAAIKS+IMTTA+ITNLEG PI+DE LQPADLFA GAGHVNPSKA
Sbjct: 541 GIAALIKSSHPDWSPAAIKSAIMTTADITNLEGQPIVDENLQPADLFATGAGHVNPSKAA 600
Query: 601 DPGLVYDIQPDDYIPYLCGLGYTNNQVSLIAHKPIDCLTTSSIPEGELNYPSFMVKLGPV 660
DPGLVYDIQPDDYIPYLCGLGY +N+V+ IA KPI+CL SIPEG+LNYPSF V LGP
Sbjct: 601 DPGLVYDIQPDDYIPYLCGLGYKSNEVATIARKPINCLAKPSIPEGDLNYPSFTVVLGPP 660
Query: 661 QTFSRTVTSVGSGRVVYNVVIEAPEGVSVTVRPRKLSFSALNQKATYSVTFKRIGSISPS 720
QTF+RTVT+VG GR VY V+EAP +SVT+RP K+ FS +NQK T+SVTFKRIGSISPS
Sbjct: 661 QTFTRTVTNVGCGREVYTAVVEAPPSISVTIRPSKIFFSKINQKVTFSVTFKRIGSISPS 720
Query: 721 IEFAEGYLKWVSAKHVVRSPISVKF 745
EF +GYLKWVS KHVVRSPIS KF
Sbjct: 721 TEFGKGYLKWVSDKHVVRSPISFKF 737
BLAST of Cmc09g0257051 vs. ExPASy TrEMBL
Match:
A0A6J1E0E3 (subtilisin-like protease SBT1.7 OS=Momordica charantia OX=3673 GN=LOC111025997 PE=3 SV=1)
HSP 1 Score: 1179.9 bits (3051), Expect = 0.0e+00
Identity = 607/756 (80.29%), Postives = 658/756 (87.04%), Query Frame = 0
Query: 1 MVLLPSLFLLLLLNFHGYEAQVTELPLSNLHTYIVHVKKPE------VVDDLEIWHRSFL 60
MV+LPSLFLL+L +FHG A+ T SNL TYIVHVKKPE V+DLE WHRSFL
Sbjct: 1 MVVLPSLFLLVLFHFHGGGARPTS---SNLQTYIVHVKKPEKLSTYGQVEDLENWHRSFL 60
Query: 61 PTSLDNEEEQPTLLYSYRNVMSGFSARLTEEHVKAMEEKDGFVSARRETIVHLHTTHTPD 120
P+S E+ P LLYS+ NVMSGF+ARLTEEHVKAMEEKDGF+SARRE I+ LHTTHTP+
Sbjct: 61 PSS---SEDHPKLLYSFXNVMSGFAARLTEEHVKAMEEKDGFISARRERILKLHTTHTPN 120
Query: 121 FLGLNRQFGFWKDSNFGKGVIIGVLDGGITPNHPSFDDAGMPQPPAKWKGRCEFNFSACN 180
FLGLNRQFGFWKDSNFGKG IIGVLDGGITPNHPSFDDAGMP PP KWKGRCEFNFSACN
Sbjct: 121 FLGLNRQFGFWKDSNFGKGXIIGVLDGGITPNHPSFDDAGMPPPPVKWKGRCEFNFSACN 180
Query: 181 NKLIGARSMNLASQALKGKITTLDDSPIDEDGHGTHTASTAAGTFVDGAEALGNAFGTAV 240
NKLIGARS NLA++ALKG+ T DSPIDEDGHGTHTASTAAG FVD AEALGNA GTAV
Sbjct: 181 NKLIGARSFNLATKALKGEQPTATDSPIDEDGHGTHTASTAAGAFVDEAEALGNAKGTAV 240
Query: 241 GMAPLAHLAIYKVCFGEDCSDVDILAGLDAAVEDGVDVLSISLGGPSVPFFADITAIGSF 300
GMAPLAHLAIYKVCFGEDC D DILA LDAAVEDGVDVLS+SLG S PFF D AIG+F
Sbjct: 241 GMAPLAHLAIYKVCFGEDCPDSDILAALDAAVEDGVDVLSLSLGSDSAPFFRDDIAIGAF 300
Query: 301 AAIQKGIFVSCSAANSGPFNATLSNEAPWILTVAASTIDRKITATAKLGNGEEFDGESLF 360
AA QKGIFVSCSA+NSGP NATLSNEAPWILTVAASTIDR+I ATAKLGNGEEFDGESLF
Sbjct: 301 AATQKGIFVSCSASNSGPLNATLSNEAPWILTVAASTIDRRIIATAKLGNGEEFDGESLF 360
Query: 361 QPNDFPQTL-LPLVFPGEKNETVALCAEGSLKNIDVKGKVVVCERGGGIARIAKGVEVKN 420
QP+DFP TL LPLV+ GEKNET ALC EGSLK+IDVKGK VVCERGGGIARIAKG+EVKN
Sbjct: 361 QPSDFPPTLFLPLVYAGEKNETAALCGEGSLKDIDVKGKAVVCERGGGIARIAKGMEVKN 420
Query: 421 GGGAAMILLNAESDGFTTEVDAHVLPASHVSHTAALKIKAYINSTTYPTATILFKGTTIG 480
GGAAMILLN ++DGFTTE DAHVLPA+HV+H AALKIK YINST P A I F+GT IG
Sbjct: 421 AGGAAMILLNQQTDGFTTEADAHVLPATHVNHVAALKIKDYINSTHNPIAAIAFRGTVIG 480
Query: 481 -DDFSPAIASFSSRGPSLASPGILKPDITGPGVSILAAWPFPLDNNT-NTKSTFNIISGT 540
DDFSPAIASFSSRGPSLASPGILKPDITGPG+SILAAWPFPLDNNT NTK TFN+ISGT
Sbjct: 481 DDDFSPAIASFSSRGPSLASPGILKPDITGPGISILAAWPFPLDNNTKNTKPTFNVISGT 540
Query: 541 SMSCPHLSGIAALIKSAHPDWSPAAIKSSIMTTANITNLEGNPILDETLQPADLFAIGAG 600
SMSCPHLSGIAALIKS+HPDWSPAAIKS+IMTTA+ITN +G PILD+ QPADLFA GAG
Sbjct: 541 SMSCPHLSGIAALIKSSHPDWSPAAIKSAIMTTADITNHKGRPILDQNFQPADLFATGAG 600
Query: 601 HVNPSKAIDPGLVYDIQPDDYIPYLCGLGYTNNQVSLIAHKPIDCLTTSSIPEGELNYPS 660
HVNPSKA DPGLVYDIQPDDYIPYLCGLGY +N+VS+IAH+P+DC SIPEGELNYPS
Sbjct: 601 HVNPSKATDPGLVYDIQPDDYIPYLCGLGYKDNEVSIIAHQPVDCSAKPSIPEGELNYPS 660
Query: 661 FMVKLGPVQTFSRTVTSVGSGRVVYNVVIEA-PEGVSVTVRPRKLSFSALNQKATYSVTF 720
F V LGP QTF+RTVT+VG G VY V+IEA P GVSVTVRPRK+ FSA+NQK YSVTF
Sbjct: 661 FSVALGPPQTFTRTVTNVGGGYEVYTVMIEAPPSGVSVTVRPRKIFFSAVNQKVAYSVTF 720
Query: 721 KRIGSIS-PSIEFAEGYLKWVSAKHVVRSPISVKFV 746
KRIGSI+ PS A+ YLK +S+KH+VRSPIS+KFV
Sbjct: 721 KRIGSITPPSSGSAQAYLKCMSSKHIVRSPISIKFV 750
BLAST of Cmc09g0257051 vs. TAIR 10
Match:
AT1G04110.1 (Subtilase family protein )
HSP 1 Score: 583.6 bits (1503), Expect = 2.3e-166
Identity = 332/734 (45.23%), Postives = 455/734 (61.99%), Query Frame = 0
Query: 49 WHRSFLPTS---LDNEEEQPT--LLYSYRNVMSGFSARLTEEHVKAMEEKDGFVSARRET 108
WH SFL + ++ EEE+P+ LLYSY + + GF+A+LTE + + V+ R +
Sbjct: 48 WHLSFLQEAVLGVEEEEEEPSSRLLYSYGSAIEGFAAQLTESEAEILRYSPEVVAVRPDH 107
Query: 109 IVHLHTTHTPDFLGLN--RQFGFWKDSNFGKGVIIGVLDGGITPNHPSFDDAGMPQPPAK 168
++ + TT++ FLGL+ G W S FG+G IIGVLD G+ P PSFDD GMP P K
Sbjct: 108 VLQVQTTYSYKFLGLDGFGNSGVWSKSRFGQGTIIGVLDTGVWPESPSFDDTGMPSIPRK 167
Query: 169 WKGRCE----FNFSACNNKLIGARSMNLASQALKGKITTLDDSP---------IDEDGHG 228
WKG C+ F+ S+CN KLIGAR + ++SP D GHG
Sbjct: 168 WKGICQEGESFSSSSCNRKLIGARFFIRGHRVANSP----EESPNMPREYISARDSTGHG 227
Query: 229 THTASTAAGTFVDGAEALGNAFGTAVGMAPLAHLAIYKVCFGEDCSDVDILAGLDAAVED 288
THTAST G+ V A LGN G A GMAP AH+A+YKVC+ C DILA +D A++D
Sbjct: 228 THTASTVGGSSVSMANVLGNGAGVARGMAPGAHIAVYKVCWFNGCYSSDILAAIDVAIQD 287
Query: 289 GVDVLSISLGGPSVPFFADITAIGSFAAIQKGIFVSCSAANSGPFNATLSNEAPWILTVA 348
VDVLS+SLGG +P + D AIG+F A+++GI V C+A N+GP ++++N APW+ T+
Sbjct: 288 KVDVLSLSLGGFPIPLYDDTIAIGTFRAMERGISVICAAGNNGPIESSVANTAPWVSTIG 347
Query: 349 ASTIDRKITATAKLGNGEEFDGESLFQ----PNDFPQTLLPLVFPGEKNETVALCAEGSL 408
A T+DR+ A +L NG+ GESL+ N + + V G+K C GSL
Sbjct: 348 AGTLDRRFPAVVRLANGKLLYGESLYPGKGIKNAGREVEVIYVTGGDKGS--EFCLRGSL 407
Query: 409 KNIDVKGKVVVCERGGGIARIAKGVEVKNGGGAAMILLNAESDGFTTEVDAHVLPASHVS 468
+++GK+V+C+RG R KG VK GG AMIL N E + +D H+LPA+ +
Sbjct: 408 PREEIRGKMVICDRGVN-GRSEKGEAVKEAGGVAMILANTEINQEEDSIDVHLLPATLIG 467
Query: 469 HTAALKIKAYINSTTYPTATILFKGTTIGDDFSPAIASFSSRGPSLASPGILKPDITGPG 528
+T ++ +KAY+N+T P A I+F GT IG +P +A FS+RGPSLA+P ILKPD+ PG
Sbjct: 468 YTESVLLKAYVNATVKPKARIIFGGTVIGRSRAPEVAQFSARGPSLANPSILKPDMIAPG 527
Query: 529 VSILAAWP-------FPLDNNTNTKSTFNIISGTSMSCPHLSGIAALIKSAHPDWSPAAI 588
V+I+AAWP P D+ + F ++SGTSMSCPH+SGI ALI+SA+P+WSPAAI
Sbjct: 528 VNIIAAWPQNLGPTGLPYDSR---RVNFTVMSGTSMSCPHVSGITALIRSAYPNWSPAAI 587
Query: 589 KSSIMTTANITNLEGNPILDETLQPADLFAIGAGHVNPSKAIDPGLVYDIQPDDYIPYLC 648
KS++MTTA++ + +G I D +PA +FAIGAGHVNP KAI+PGLVY+IQP DYI YLC
Sbjct: 588 KSALMTTADLYDRQGKAIKDGN-KPAGVFAIGAGHVNPQKAINPGLVYNIQPVDYITYLC 647
Query: 649 GLGYTNNQVSLIAHKPIDCL-TTSSIPEGELNYPSFMV--KLG-PVQTFSRTVTSVGSGR 708
LG+T + + I HK + C P LNYPS V K G + +R VT+VGS
Sbjct: 648 TLGFTRSDILAITHKNVSCNGILRKNPGFSLNYPSIAVIFKRGKTTEMITRRVTNVGSPN 707
Query: 709 VVYNVVIEAPEGVSVTVRPRKLSFSALNQKATYSVTF--KRIGSISPSIEFAEGYLKWVS 743
+Y+V ++APEG+ V V P++L F ++Q +Y V F K+ FA+G L WV+
Sbjct: 708 SIYSVNVKAPEGIKVIVNPKRLVFKHVDQTLSYRVWFVLKKKNRGGKVASFAQGQLTWVN 767
BLAST of Cmc09g0257051 vs. TAIR 10
Match:
AT5G67360.1 (Subtilase family protein )
HSP 1 Score: 578.9 bits (1491), Expect = 5.7e-165
Identity = 334/756 (44.18%), Postives = 460/756 (60.85%), Query Frame = 0
Query: 6 SLFLLLLLNFHGYEAQVTELPLSNLHTYIVHVKKPEVVDDLEIWHRSFLPTSLDNEEEQP 65
+ FLLL L F + S+ TYIVH+ K ++ ++ H ++ +SL + +
Sbjct: 10 AFFLLLCLGFCHVSSS-----SSDQGTYIVHMAKSQMPSSFDL-HSNWYDSSLRSISDSA 69
Query: 66 TLLYSYRNVMSGFSARLTEEHVKAMEEKDGFVSARRETIVHLHTTHTPDFLGLNRQFG-F 125
LLY+Y N + GFS RLT+E ++ + G +S E LHTT TP FLGL+
Sbjct: 70 ELLYTYENAIHGFSTRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLGLDEHTADL 129
Query: 126 WKDSNFGKGVIIGVLDGGITPNHPSFDDAGMPQPPAKWKGRCE----FNFSACNNKLIGA 185
+ ++ V++GVLD G+ P S+ D G P+ WKG CE F S CN KLIGA
Sbjct: 130 FPEAGSYSDVVVGVLDTGVWPESKSYSDEGFGPIPSSWKGGCEAGTNFTASLCNRKLIGA 189
Query: 186 RSMNLASQALKGKITTLDD--SPIDEDGHGTHTASTAAGTFVDGAEALGNAFGTAVGMAP 245
R ++ G I + SP D+DGHGTHT+STAAG+ V+GA LG A GTA GMAP
Sbjct: 190 RFFARGYESTMGPIDESKESRSPRDDDGHGTHTSSTAAGSVVEGASLLGYASGTARGMAP 249
Query: 246 LAHLAIYKVCFGEDCSDVDILAGLDAAVEDGVDVLSISLGGPSVPFFADITAIGSFAAIQ 305
A +A+YKVC+ C DILA +D A+ D V+VLS+SLGG ++ D AIG+FAA++
Sbjct: 250 RARVAVYKVCWLGGCFSSDILAAIDKAIADNVNVLSMSLGGGMSDYYRDGVAIGAFAAME 309
Query: 306 KGIFVSCSAANSGPFNATLSNEAPWILTVAASTIDRKITATAKLGNGEEFDGESLFQPND 365
+GI VSCSA N+GP +++LSN APWI TV A T+DR A A LGNG+ F G SLF+
Sbjct: 310 RGILVSCSAGNAGPSSSSLSNVAPWITTVGAGTLDRDFPALAILGNGKNFTGVSLFKGEA 369
Query: 366 FPQTLLPLVFPGEKNETV--ALCAEGSLKNIDVKGKVVVCERGGGIARIAKGVEVKNGGG 425
P LLP ++ G + LC G+L VKGK+V+C+RG AR+ KG VK GG
Sbjct: 370 LPDKLLPFIYAGNASNATNGNLCMTGTLIPEKVKGKIVMCDRGIN-ARVQKGDVVKAAGG 429
Query: 426 AAMILLNAESDGFTTEVDAHVLPASHVSHTAALKIKAYINSTTYPTATILFKGTTIGDDF 485
MIL N ++G DAH+LPA+ V A I+ Y+ + PTA+I GT +G
Sbjct: 430 VGMILANTAANGEELVADAHLLPATTVGEKAGDIIRHYVTTDPNPTASISILGTVVGVKP 489
Query: 486 SPAIASFSSRGPSLASPGILKPDITGPGVSILAAW-----PFPLDNNTNTKSTFNIISGT 545
SP +A+FSSRGP+ +P ILKPD+ PGV+ILAAW P L +++ + FNIISGT
Sbjct: 490 SPVVAAFSSRGPNSITPNILKPDLIAPGVNILAAWTGAAGPTGLASDSR-RVEFNIISGT 549
Query: 546 SMSCPHLSGIAALIKSAHPDWSPAAIKSSIMTTANITNLEGNPILD-ETLQPADLFAIGA 605
SMSCPH+SG+AAL+KS HP+WSPAAI+S++MTTA T +G P+LD T +P+ F GA
Sbjct: 550 SMSCPHVSGLAALLKSVHPEWSPAAIRSALMTTAYKTYKDGKPLLDIATGKPSTPFDHGA 609
Query: 606 GHVNPSKAIDPGLVYDIQPDDYIPYLCGLGYTNNQVSLIAHKPIDCLTTSSIPEGELNYP 665
GHV+P+ A +PGL+YD+ +DY+ +LC L YT+ Q+ ++ + C + S +LNYP
Sbjct: 610 GHVSPTTATNPGLIYDLTTEDYLGFLCALNYTSPQIRSVSRRNYTCDPSKSYSVADLNYP 669
Query: 666 SFMVKLGPV--QTFSRTVTSVGSGRVVYNVVIEAPEGVSVTVRPRKLSFSALNQKATYSV 725
SF V + V ++RTVTSVG V GV ++V P L+F N+K +Y+V
Sbjct: 670 SFAVNVDGVGAYKYTRTVTSVGGAGTYSVKVTSETTGVKISVEPAVLNFKEANEKKSYTV 729
Query: 726 TFKRIGSISPSIEFAEGYLKWVSAKHVVRSPISVKF 745
TF + S PS + G ++W KHVV SP+++ +
Sbjct: 730 TF-TVDSSKPSGSNSFGSIEWSDGKHVVGSPVAISW 756
BLAST of Cmc09g0257051 vs. TAIR 10
Match:
AT3G14240.1 (Subtilase family protein )
HSP 1 Score: 576.6 bits (1485), Expect = 2.8e-164
Identity = 340/751 (45.27%), Postives = 450/751 (59.92%), Query Frame = 0
Query: 28 SNLHTYIVHV---KKPEVVDDLEIWHRSFLPTSLDNEEEQPTLLYSYRNVMSGFSARLTE 87
SN TYIVHV KP + W+ S L + + P+++++Y V GFSARLT
Sbjct: 23 SNSLTYIVHVDHEAKPSIFPTHFHWYTSSLASLTSS---PPSIIHTYDTVFHGFSARLTS 82
Query: 88 EHVKAMEEKDGFVSARRETIVHLHTTHTPDFLGLNR--QFGFWKDSNFGKGVIIGVLDGG 147
+ + + +S E + HLHTT +P+FLGL + G ++S+FG ++IGV+D G
Sbjct: 83 QDASQLLDHPHVISVIPEQVRHLHTTRSPEFLGLRSTDKAGLLEESDFGSDLVIGVIDTG 142
Query: 148 ITPNHPSFDDAGMPQPPAKWKGRC----EFNFSACNNKLIGARSMNLASQALKGKI--TT 207
+ P PSFDD G+ P KWKG+C +F SACN KL+GAR +A GK+ TT
Sbjct: 143 VWPERPSFDDRGLGPVPIKWKGQCIASQDFPESACNRKLVGARFFCGGYEATNGKMNETT 202
Query: 208 LDDSPIDEDGHGTHTASTAAGTFVDGAEALGNAFGTAVGMAPLAHLAIYKVCFGEDCSDV 267
SP D DGHGTHTAS +AG +V A LG A G A GMAP A LA YKVC+ C D
Sbjct: 203 EFRSPRDSDGHGTHTASISAGRYVFPASTLGYAHGVAAGMAPKARLAAYKVCWNSGCYDS 262
Query: 268 DILAGLDAAVEDGVDVLSISLGGPSVPFFADITAIGSFAAIQKGIFVSCSAANSGPFNAT 327
DILA D AV DGVDV+S+S+GG VP++ D AIG+F AI +GIFVS SA N GP T
Sbjct: 263 DILAAFDTAVADGVDVISLSVGGVVVPYYLDAIAIGAFGAIDRGIFVSASAGNGGPGALT 322
Query: 328 LSNEAPWILTVAASTIDRKITATAKLGNGEEFDGESLF-QPNDFPQTLLPLVFPGE---- 387
++N APW+ TV A TIDR A KLGNG+ G S++ P P + PLV+ G
Sbjct: 323 VTNVAPWMTTVGAGTIDRDFPANVKLGNGKMISGVSVYGGPGLDPGRMYPLVYGGSLLGG 382
Query: 388 KNETVALCAEGSLKNIDVKGKVVVCERGGGIARIAKGVEVKNGGGAAMILLNAESDGFTT 447
+ +LC EGSL VKGK+V+C+RG +R KG V+ GG MI+ N DG
Sbjct: 383 DGYSSSLCLEGSLDPNLVKGKIVLCDRGIN-SRATKGEIVRKNGGLGMIIANGVFDGEGL 442
Query: 448 EVDAHVLPASHVSHTAALKIKAYIN------STTYPTATILFKGTTIGDDFSPAIASFSS 507
D HVLPA+ V + +I+ YI+ S+ +PTATI+FKGT +G +P +ASFS+
Sbjct: 443 VADCHVLPATSVGASGGDEIRRYISESSKSRSSKHPTATIVFKGTRLGIRPAPVVASFSA 502
Query: 508 RGPSLASPGILKPDITGPGVSILAAWPFPLD----NNTNTKSTFNIISGTSMSCPHLSGI 567
RGP+ +P ILKPD+ PG++ILAAWP + + N ++ FNI+SGTSM+CPH+SG+
Sbjct: 503 RGPNPETPEILKPDVIAPGLNILAAWPDRIGPSGVTSDNRRTEFNILSGTSMACPHVSGL 562
Query: 568 AALIKSAHPDWSPAAIKSSIMTTANITNLEGNPILDE-TLQPADLFAIGAGHVNPSKAID 627
AAL+K+AHPDWSPAAI+S+++TTA + G P++DE T + + G+GHV+P+KA+D
Sbjct: 563 AALLKAAHPDWSPAAIRSALITTAYTVDNSGEPMMDESTGNTSSVMDYGSGHVHPTKAMD 622
Query: 628 PGLVYDIQPDDYIPYLCGLGYTNNQVSLIAHKPIDCLTTSSIPE-GELNYPSFMVKL--- 687
PGLVYDI DYI +LC YT + I + DC G LNYPSF V
Sbjct: 623 PGLVYDITSYDYINFLCNSNYTRTNIVTITRRQADCDGARRAGHVGNLNYPSFSVVFQQY 682
Query: 688 --GPVQT-FSRTVTSVGSGRVVYNVVIEAPEGVSVTVRPRKLSFSALNQKATYSVTFKRI 743
+ T F RTVT+VG VY + I P G +VTV P KLSF + QK ++ V K
Sbjct: 683 GESKMSTHFIRTVTNVGDSDSVYEIKIRPPRGTTVTVEPEKLSFRRVGQKLSFVVRVKTT 742
BLAST of Cmc09g0257051 vs. TAIR 10
Match:
AT2G05920.1 (Subtilase family protein )
HSP 1 Score: 567.0 bits (1460), Expect = 2.2e-161
Identity = 319/727 (43.88%), Postives = 445/727 (61.21%), Query Frame = 0
Query: 32 TYIVHVKKPEVVDDLEIWHRSFLPTSLDNEEEQPTLLYSYRNVMSGFSARL-TEEHVKAM 91
TYI+ V + + + H + + L++E +LLY+Y GFSA L + E +
Sbjct: 29 TYIIRVNHSDKPESF-LTHHDWYTSQLNSES---SLLYTYTTSFHGFSAYLDSTEADSLL 88
Query: 92 EEKDGFVSARRETIVHLHTTHTPDFLGLNRQFGFWKDSNFGKGVIIGVLDGGITPNHPSF 151
+ + + + LHTT TP+FLGLN +FG + GVIIGVLD G+ P SF
Sbjct: 89 SSSNSILDIFEDPLYTLHTTRTPEFLGLNSEFGVHDLGSSSNGVIIGVLDTGVWPESRSF 148
Query: 152 DDAGMPQPPAKWKGRCE----FNFSACNNKLIGARSMNLASQALKG---KITTLDDSPID 211
DD MP+ P+KWKG CE F+ CN KLIGARS + Q G SP D
Sbjct: 149 DDTDMPEIPSKWKGECESGSDFDSKLCNKKLIGARSFSKGFQMASGGGFSSKRESVSPRD 208
Query: 212 EDGHGTHTASTAAGTFVDGAEALGNAFGTAVGMAPLAHLAIYKVCFGEDCSDVDILAGLD 271
DGHGTHT++TAAG+ V A LG A GTA GMA A +A YKVC+ C DILA +D
Sbjct: 209 VDGHGTHTSTTAAGSAVRNASFLGYAAGTARGMATRARVATYKVCWSTGCFGSDILAAMD 268
Query: 272 AAVEDGVDVLSISLGGPSVPFFADITAIGSFAAIQKGIFVSCSAANSGPFNATLSNEAPW 331
A+ DGVDVLS+SLGG S P++ D AIG+F+A+++G+FVSCSA NSGP A+++N APW
Sbjct: 269 RAILDGVDVLSLSLGGGSAPYYRDTIAIGAFSAMERGVFVSCSAGNSGPTRASVANVAPW 328
Query: 332 ILTVAASTIDRKITATAKLGNGEEFDGESLFQPNDFPQTLLPLVFPGEKNETVALCAEGS 391
++TV A T+DR A A LGNG+ G SL+ L LV+ + + LC GS
Sbjct: 329 VMTVGAGTLDRDFPAFANLGNGKRLTGVSLYSGVGMGTKPLELVYNKGNSSSSNLCLPGS 388
Query: 392 LKNIDVKGKVVVCERGGGIARIAKGVEVKNGGGAAMILLNAESDGFTTEVDAHVLPASHV 451
L + V+GK+VVC+RG AR+ KG V++ GG MI+ N + G D+H+LPA V
Sbjct: 389 LDSSIVRGKIVVCDRGVN-ARVEKGAVVRDAGGLGMIMANTAASGEELVADSHLLPAIAV 448
Query: 452 SHTAALKIKAYINSTTYPTATILFKGTTIGDDFSPAIASFSSRGPSLASPGILKPDITGP 511
++ Y+ S + PTA ++FKGT + SP +A+FSSRGP+ +P ILKPD+ GP
Sbjct: 449 GKKTGDLLREYVKSDSKPTALLVFKGTVLDVKPSPVVAAFSSRGPNTVTPEILKPDVIGP 508
Query: 512 GVSILAAW-----PFPLDNNTNTKSTFNIISGTSMSCPHLSGIAALIKSAHPDWSPAAIK 571
GV+ILA W P LD ++ ++ FNI+SGTSMSCPH+SG+A L+K+AHP+WSP+AIK
Sbjct: 509 GVNILAGWSDAIGPTGLDKDSR-RTQFNIMSGTSMSCPHISGLAGLLKAAHPEWSPSAIK 568
Query: 572 SSIMTTANITNLEGNPILDETLQP-ADLFAIGAGHVNPSKAIDPGLVYDIQPDDYIPYLC 631
S++MTTA + + P+ D ++ +A G+GHV+P KA+ PGLVYDI ++YI +LC
Sbjct: 569 SALMTTAYVLDNTNAPLHDAADNSLSNPYAHGSGHVDPQKALSPGLVYDISTEEYIRFLC 628
Query: 632 GLGYTNNQVSLIAHKP-IDCLTTSSIPEGELNYPSFMVKLG--PVQTFSRTVTSVGSGRV 691
L YT + + I +P ++C S P G+LNYPSF V G V ++R VT+VG+
Sbjct: 629 SLDYTVDHIVAIVKRPSVNCSKKFSDP-GQLNYPSFSVLFGGKRVVRYTREVTNVGAASS 688
Query: 692 VYNVVIEAPEGVSVTVRPRKLSFSALNQKATYSVTFKRIGSISPSIEFAEGYLKWVSAKH 742
VY V + V ++V+P KLSF ++ +K Y+VTF +S + + G + W + +H
Sbjct: 689 VYKVTVNGAPSVGISVKPSKLSFKSVGEKKRYTVTFVSKKGVSMTNKAEFGSITWSNPQH 748
BLAST of Cmc09g0257051 vs. TAIR 10
Match:
AT3G14067.1 (Subtilase family protein )
HSP 1 Score: 565.5 bits (1456), Expect = 6.5e-161
Identity = 346/776 (44.59%), Postives = 457/776 (58.89%), Query Frame = 0
Query: 4 LPSLFLL--LLLNFHGYEAQVTELPLSNLHTYIVHVK---KPEVVDDLEIWHRSFLPTSL 63
L S+F + LLL F + ++ L +YIVHV+ KP + WH S L SL
Sbjct: 6 LSSIFFVFPLLLCFFSPSSSSSD----GLESYIVHVQRSHKPSLFSSHNNWHVSLL-RSL 65
Query: 64 DNEEEQPTLLYSYRNVMSGFSARLTEEHVKAMEEKDGFVSARRETIVHLHTTHTPDFLGL 123
+ + TLLYSY + GFSARL+ A+ +S + +HTTHTP FLG
Sbjct: 66 PSSPQPATLLYSYSRAVHGFSARLSPIQTAALRRHPSVISVIPDQAREIHTTHTPAFLGF 125
Query: 124 NRQFGFWKDSNFGKGVIIGVLDGGITPNHPSFDDAGMPQPPAKWKGRCE----FNFSACN 183
++ G W +SN+G+ VI+GVLD GI P HPSF D+G+ P+ WKG CE F S+CN
Sbjct: 126 SQNSGLWSNSNYGEDVIVGVLDTGIWPEHPSFSDSGLGPIPSTWKGECEIGPDFPASSCN 185
Query: 184 NKLIGARSMN----LASQALKGKITTLDDSPIDEDGHGTHTASTAAGTFVDGAEALGNAF 243
KLIGAR+ K SP D +GHGTHTASTAAG+ V A A
Sbjct: 186 RKLIGARAFYRGYLTQRNGTKKHAAKESRSPRDTEGHGTHTASTAAGSVVANASLYQYAR 245
Query: 244 GTAVGMAPLAHLAIYKVCFGEDCSDVDILAGLDAAVEDGVDVLSISLG--GPSVPFFADI 303
GTA GMA A +A YK+C+ C D DILA +D AV DGV V+S+S+G G + + D
Sbjct: 246 GTATGMASKARIAAYKICWTGGCYDSDILAAMDQAVADGVHVISLSVGASGSAPEYHTDS 305
Query: 304 TAIGSFAAIQKGIFVSCSAANSGPFNATLSNEAPWILTVAASTIDRKITATAKLGNGEEF 363
AIG+F A + GI VSCSA NSGP T +N APWILTV AST+DR+ A A G+G+ F
Sbjct: 306 IAIGAFGATRHGIVVSCSAGNSGPNPETATNIAPWILTVGASTVDREFAANAITGDGKVF 365
Query: 364 DGESLFQPNDFPQTLLPLVFPGEKNETVALCAEGSLKNIDVKGKVVVCERGGGIARIAKG 423
G SL+ P + L LV+ G+ LC G L + V+GK+V+C+RGG AR+ KG
Sbjct: 366 TGTSLYAGESLPDSQLSLVYSGDCGS--RLCYPGKLNSSLVEGKIVLCDRGGN-ARVEKG 425
Query: 424 VEVKNGGGAAMILLNAESDGFTTEVDAHVLPASHVSHTAALKIKAYINSTTYPTATILFK 483
VK GGA MIL N G D+H++PA+ V A +I+ YI ++ PTA I F
Sbjct: 426 SAVKLAGGAGMILANTAESGEELTADSHLVPATMVGAKAGDQIRDYIKTSDSPTAKISFL 485
Query: 484 GTTIG-DDFSPAIASFSSRGPSLASPGILKPDITGPGVSILAAW-----PFPLDNNTNTK 543
GT IG SP +A+FSSRGP+ +P ILKPD+ PGV+ILA W P LD + +
Sbjct: 486 GTLIGPSPPSPRVAAFSSRGPNHLTPVILKPDVIAPGVNILAGWTGMVGPTDLDIDPR-R 545
Query: 544 STFNIISGTSMSCPHLSGIAALIKSAHPDWSPAAIKSSIMTTANITNLEGNPILD-ETLQ 603
FNIISGTSMSCPH+SG+AAL++ AHPDWSPAAIKS+++TTA G PI D T +
Sbjct: 546 VQFNIISGTSMSCPHVSGLAALLRKAHPDWSPAAIKSALVTTAYDVENSGEPIEDLATGK 605
Query: 604 PADLFAIGAGHVNPSKAIDPGLVYDIQPDDYIPYLCGLGYTNNQVSLIAHKPI---DCLT 663
++ F GAGHV+P+KA++PGLVYDI+ +Y+ +LC +GY + + P C T
Sbjct: 606 SSNSFIHGAGHVDPNKALNPGLVYDIEVKEYVAFLCAVGYEFPGILVFLQDPTLYDACET 665
Query: 664 TSSIPEGELNYPSFMV---KLGPVQTFSRTVTSVGSG-RVVYNVVIEAPEGVSVTVRPRK 723
+ G+LNYPSF V G V + R V +VGS VY V +++P V + V P K
Sbjct: 666 SKLRTAGDLNYPSFSVVFASTGEVVKYKRVVKNVGSNVDAVYEVGVKSPANVEIDVSPSK 725
Query: 724 LSFSALNQKATYSVTFKR------IGSISPSIEFAEGYLKWVSAKHVVRSPISVKF 745
L+FS Y VTFK +GS+ P EF G ++W +HVV+SP++V++
Sbjct: 726 LAFSKEKSVLEYEVTFKSVVLGGGVGSV-PGHEF--GSIEWTDGEHVVKSPVAVQW 769
The following BLAST results are available for this feature:
Match Name | E-value | Identity | Description | |
KAA0042818.1 | 0.0e+00 | 100.00 | subtilisin-like protease SBT1.7 [Cucumis melo var. makuwa] | [more] |
XP_008437181.1 | 0.0e+00 | 99.73 | PREDICTED: subtilisin-like protease SBT1.7 [Cucumis melo] | [more] |
KGN50165.1 | 0.0e+00 | 95.70 | hypothetical protein Csa_000664 [Cucumis sativus] | [more] |
XP_038876087.1 | 0.0e+00 | 88.92 | LOW QUALITY PROTEIN: subtilisin-like protease 4 [Benincasa hispida] | [more] |
XP_022958542.1 | 0.0e+00 | 83.62 | subtilisin-like protease SBT1.8 [Cucurbita moschata] | [more] |
Match Name | E-value | Identity | Description | |
A9QY38 | 1.3e-283 | 65.56 | Subtilisin-like protease 4 OS=Lotus japonicus OX=34305 GN=SBTM4 PE=2 SV=1 | [more] |
A0A0M3R8G2 | 3.3e-250 | 60.78 | Subtilisin-like protease OS=Petunia hybrida OX=4102 GN=SBT PE=2 SV=1 | [more] |
A9QY40 | 4.7e-241 | 60.63 | Subtilisin-like protease 1 OS=Lotus japonicus OX=34305 GN=SBTM1 PE=2 SV=1 | [more] |
A9QY39 | 8.7e-235 | 58.28 | Subtilisin-like protease 3 OS=Lotus japonicus OX=34305 GN=SBTM3 PE=2 SV=1 | [more] |
G7KEU7 | 1.2e-228 | 56.54 | Subtilisin-like protease OS=Medicago truncatula OX=3880 GN=SBT PE=2 SV=1 | [more] |
Match Name | E-value | Identity | Description | |
A0A5A7TJ55 | 0.0e+00 | 100.00 | Subtilisin-like protease SBT1.7 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_... | [more] |
A0A1S3ATZ7 | 0.0e+00 | 99.73 | subtilisin-like protease SBT1.7 OS=Cucumis melo OX=3656 GN=LOC103482686 PE=3 SV=... | [more] |
A0A0A0KKE3 | 0.0e+00 | 95.70 | Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G157240 PE=3 SV=1 | [more] |
A0A6J1H242 | 0.0e+00 | 83.62 | subtilisin-like protease SBT1.8 OS=Cucurbita moschata OX=3662 GN=LOC111459745 PE... | [more] |
A0A6J1E0E3 | 0.0e+00 | 80.29 | subtilisin-like protease SBT1.7 OS=Momordica charantia OX=3673 GN=LOC111025997 P... | [more] |