Cmc09g0251271 (gene) Melon (Charmono) v1.1

Overview
NameCmc09g0251271
Typegene
OrganismCucumis melo L. var. cantalupensis cv. Charmono (Melon (Charmono) v1.1)
DescriptionPectinesterase
LocationCMiso1.1chr09: 17342765 .. 17344823 (-)
RNA-Seq ExpressionCmc09g0251271
SyntenyCmc09g0251271
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGTTGCGAAGGTGGTCATGGCGGTAACTTCCCTTCTTGTAGTCGGTGTAGTTCTCGCTGTCTTCGCTATGGTAAACAAGAACCATAGTTCTGATGAAACCGCGGATATGTCTCCAAAAATGAAGGCTGTCTCATCTATTTGTTCTAATACCGATTACCAACAAGAATGTCAAACTACACTTGGTAATGTTGGACATAATTCGTCGGTAGATGACCCGAAAGAGTTCCTTAAAGCCGCGATTACGGCTACGATTGAAGAGATGAAAAAGGGTTATAATTTGACTGATAACCTTATGGTTGAGGCAGCTAATAATGCTTCAATTAAAATGACTGTTGATGATTGTAGGGATTTATTACAATCTGCTATTGATGAGTTGCATGCATCTTATTCTACTGTTGGCGATCCCGATTTGCATACTAACGAAGACCGTGTCGCTGATATTAAGAATTGGCTAACCGCTGTTATTTCTTATCAACAATCTTGTCTTGATGGACTTGAGGAATTTGATCCTCAACTTAAACAAAAGATGCAAGGTGGTCTTGATGTTGCTGCCAAGCTTACTAGTAATGCTCTTGCTATTGTTGATGCTGTTTCTGATATTCTCAAAAGCCTTGGCTTGCAATTAAAGGTACTAATAGGGTTCGATAAATTACTATCACTTTCTATTAAACTCACAGTTATTAATATTTGTTATTAATTGAATTTAAAATTTTACTCAAAATTTTATAAATATTTTCGACCTTTACCATTTAACATTTACAACTATTATTTAATAAAACATAAAATGTCATATTTAATAAAAACATAAATTTGACATTTTTAGTGGTAGGATCTACGGGACCAACAATAAAGTAGAGTTGAAAATTTCATTTCTATTTTATGTTTCCAGGTCCAACCAAGTGGTCGTAGGCTACTTGGAACAACGGAAGTAGATAACGATGGGTACCCGACGTGGTTGACGGGGGCAGACAGGAAGTTGTTGGCAGCAAAAAGAGGAGGTGTTCGAGTGAAACCGAACGCAGTGGTGGCTAAGGACGGTAGTGGACAATACAAGACCATTGCTGCTGCATTAGCTGCATACCCAAAGGCCCTAAAAGGAAGATATGTAATCTACGTGAAAGCAGGGATCTATAATGAATACATCATTGTCACTAAAGACATGAAAAATGTTTTCATGTATGGAGATGGCCCAAGAAAAACCATTGTCACCGGTCGCAAAAGCAACCGTGATGGCATCAACACCCAAAACACCGCAACTTTTGGTAAGTCTCACAAAAATTATGTGTGGTTGAATGATATAATACTAAATTTTCAACTTAGCTAATCGATGATTAAAAATACAAATTTATATACCTCTTGCAGCTGCCATCGGAGAAGGATTTCTTTGCAAATCCATGGGATTCACCAACACAGCTGGCCCCGAAGGCCATCAAGCAGTAGCCCTCAGAGTCCAATCCGACAGATCTGCCTTCTTTAATTGTAGAATGGATGGCTACCAAGACACTCTCTACGTACAAACTCACCGTCAATTCTACCGCAATTGTGTCGTATCTGGTACCGTAGATTTCATCTTTGGCGACTCAAACACCATCATCCAAAACTCATTGATCATTGTTCGCCGTCCAATGGACAACCAACAGAACACTGTTACGGCCAACGGTCGTGCCGATAAAAAAGAAATCTCTGGATTGGTGATCCATAACTGCAGAATCGTTCCTGAGCAGAAGCTATTCGCTGAGAGGTTCAAGATTCCAACATTCTTGGGGAGGCCATGGAAGGAATATGCTAGAACAGTGATCATGGAGACGACATTGGGGGACTTTATTCAGCCGGCTGGATATATGCCTTGGTCTGGGGATTTTGCATTGGCAACTTGTTCGTATTTTGAGTATGGGAACAGAGGGCCTGGGGCTAACACTAAAAGGAGAGTGAGGTGGAAGGGAGTTAGAGTGATAGGAAGAAATGAAGCCATGCAATTCACTGCTGGACCTTTTCTTTTGGGAAGAGCTTGGCTTCCAGCCACTGGTGGACCTTACCTCTTAGGGCTCAAGAATTGA

mRNA sequence

ATGGTTGCGAAGGTGGTCATGGCGGTAACTTCCCTTCTTGTAGTCGGTGTAGTTCTCGCTGTCTTCGCTATGGTAAACAAGAACCATAGTTCTGATGAAACCGCGGATATGTCTCCAAAAATGAAGGCTGTCTCATCTATTTGTTCTAATACCGATTACCAACAAGAATGTCAAACTACACTTGGTAATGTTGGACATAATTCGTCGGTAGATGACCCGAAAGAGTTCCTTAAAGCCGCGATTACGGCTACGATTGAAGAGATGAAAAAGGGTTATAATTTGACTGATAACCTTATGGTTGAGGCAGCTAATAATGCTTCAATTAAAATGACTGTTGATGATTGTAGGGATTTATTACAATCTGCTATTGATGAGTTGCATGCATCTTATTCTACTGTTGGCGATCCCGATTTGCATACTAACGAAGACCGTGTCGCTGATATTAAGAATTGGCTAACCGCTGTTATTTCTTATCAACAATCTTGTCTTGATGGACTTGAGGAATTTGATCCTCAACTTAAACAAAAGATGCAAGGTGGTCTTGATGTTGCTGCCAAGCTTACTAGTAATGCTCTTGCTATTGTTGATGCTGTTTCTGATATTCTCAAAAGCCTTGGCTTGCAATTAAAGGTCCAACCAAGTGGTCGTAGGCTACTTGGAACAACGGAAGTAGATAACGATGGGTACCCGACGTGGTTGACGGGGGCAGACAGGAAGTTGTTGGCAGCAAAAAGAGGAGGTGTTCGAGTGAAACCGAACGCAGTGGTGGCTAAGGACGGTAGTGGACAATACAAGACCATTGCTGCTGCATTAGCTGCATACCCAAAGGCCCTAAAAGGAAGATATGTAATCTACGTGAAAGCAGGGATCTATAATGAATACATCATTGTCACTAAAGACATGAAAAATGTTTTCATGTATGGAGATGGCCCAAGAAAAACCATTGTCACCGGTCGCAAAAGCAACCGTGATGGCATCAACACCCAAAACACCGCAACTTTTGCTGCCATCGGAGAAGGATTTCTTTGCAAATCCATGGGATTCACCAACACAGCTGGCCCCGAAGGCCATCAAGCAGTAGCCCTCAGAGTCCAATCCGACAGATCTGCCTTCTTTAATTGTAGAATGGATGGCTACCAAGACACTCTCTACGTACAAACTCACCGTCAATTCTACCGCAATTGTGTCGTATCTGGTACCGTAGATTTCATCTTTGGCGACTCAAACACCATCATCCAAAACTCATTGATCATTGTTCGCCGTCCAATGGACAACCAACAGAACACTGTTACGGCCAACGGTCGTGCCGATAAAAAAGAAATCTCTGGATTGGTGATCCATAACTGCAGAATCGTTCCTGAGCAGAAGCTATTCGCTGAGAGGTTCAAGATTCCAACATTCTTGGGGAGGCCATGGAAGGAATATGCTAGAACAGTGATCATGGAGACGACATTGGGGGACTTTATTCAGCCGGCTGGATATATGCCTTGGTCTGGGGATTTTGCATTGGCAACTTGTTCGTATTTTGAGTATGGGAACAGAGGGCCTGGGGCTAACACTAAAAGGAGAGTGAGGTGGAAGGGAGTTAGAGTGATAGGAAGAAATGAAGCCATGCAATTCACTGCTGGACCTTTTCTTTTGGGAAGAGCTTGGCTTCCAGCCACTGGTGGACCTTACCTCTTAGGGCTCAAGAATTGA

Coding sequence (CDS)

ATGGTTGCGAAGGTGGTCATGGCGGTAACTTCCCTTCTTGTAGTCGGTGTAGTTCTCGCTGTCTTCGCTATGGTAAACAAGAACCATAGTTCTGATGAAACCGCGGATATGTCTCCAAAAATGAAGGCTGTCTCATCTATTTGTTCTAATACCGATTACCAACAAGAATGTCAAACTACACTTGGTAATGTTGGACATAATTCGTCGGTAGATGACCCGAAAGAGTTCCTTAAAGCCGCGATTACGGCTACGATTGAAGAGATGAAAAAGGGTTATAATTTGACTGATAACCTTATGGTTGAGGCAGCTAATAATGCTTCAATTAAAATGACTGTTGATGATTGTAGGGATTTATTACAATCTGCTATTGATGAGTTGCATGCATCTTATTCTACTGTTGGCGATCCCGATTTGCATACTAACGAAGACCGTGTCGCTGATATTAAGAATTGGCTAACCGCTGTTATTTCTTATCAACAATCTTGTCTTGATGGACTTGAGGAATTTGATCCTCAACTTAAACAAAAGATGCAAGGTGGTCTTGATGTTGCTGCCAAGCTTACTAGTAATGCTCTTGCTATTGTTGATGCTGTTTCTGATATTCTCAAAAGCCTTGGCTTGCAATTAAAGGTCCAACCAAGTGGTCGTAGGCTACTTGGAACAACGGAAGTAGATAACGATGGGTACCCGACGTGGTTGACGGGGGCAGACAGGAAGTTGTTGGCAGCAAAAAGAGGAGGTGTTCGAGTGAAACCGAACGCAGTGGTGGCTAAGGACGGTAGTGGACAATACAAGACCATTGCTGCTGCATTAGCTGCATACCCAAAGGCCCTAAAAGGAAGATATGTAATCTACGTGAAAGCAGGGATCTATAATGAATACATCATTGTCACTAAAGACATGAAAAATGTTTTCATGTATGGAGATGGCCCAAGAAAAACCATTGTCACCGGTCGCAAAAGCAACCGTGATGGCATCAACACCCAAAACACCGCAACTTTTGCTGCCATCGGAGAAGGATTTCTTTGCAAATCCATGGGATTCACCAACACAGCTGGCCCCGAAGGCCATCAAGCAGTAGCCCTCAGAGTCCAATCCGACAGATCTGCCTTCTTTAATTGTAGAATGGATGGCTACCAAGACACTCTCTACGTACAAACTCACCGTCAATTCTACCGCAATTGTGTCGTATCTGGTACCGTAGATTTCATCTTTGGCGACTCAAACACCATCATCCAAAACTCATTGATCATTGTTCGCCGTCCAATGGACAACCAACAGAACACTGTTACGGCCAACGGTCGTGCCGATAAAAAAGAAATCTCTGGATTGGTGATCCATAACTGCAGAATCGTTCCTGAGCAGAAGCTATTCGCTGAGAGGTTCAAGATTCCAACATTCTTGGGGAGGCCATGGAAGGAATATGCTAGAACAGTGATCATGGAGACGACATTGGGGGACTTTATTCAGCCGGCTGGATATATGCCTTGGTCTGGGGATTTTGCATTGGCAACTTGTTCGTATTTTGAGTATGGGAACAGAGGGCCTGGGGCTAACACTAAAAGGAGAGTGAGGTGGAAGGGAGTTAGAGTGATAGGAAGAAATGAAGCCATGCAATTCACTGCTGGACCTTTTCTTTTGGGAAGAGCTTGGCTTCCAGCCACTGGTGGACCTTACCTCTTAGGGCTCAAGAATTGA

Protein sequence

MVAKVVMAVTSLLVVGVVLAVFAMVNKNHSSDETADMSPKMKAVSSICSNTDYQQECQTTLGNVGHNSSVDDPKEFLKAAITATIEEMKKGYNLTDNLMVEAANNASIKMTVDDCRDLLQSAIDELHASYSTVGDPDLHTNEDRVADIKNWLTAVISYQQSCLDGLEEFDPQLKQKMQGGLDVAAKLTSNALAIVDAVSDILKSLGLQLKVQPSGRRLLGTTEVDNDGYPTWLTGADRKLLAAKRGGVRVKPNAVVAKDGSGQYKTIAAALAAYPKALKGRYVIYVKAGIYNEYIIVTKDMKNVFMYGDGPRKTIVTGRKSNRDGINTQNTATFAAIGEGFLCKSMGFTNTAGPEGHQAVALRVQSDRSAFFNCRMDGYQDTLYVQTHRQFYRNCVVSGTVDFIFGDSNTIIQNSLIIVRRPMDNQQNTVTANGRADKKEISGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKEYARTVIMETTLGDFIQPAGYMPWSGDFALATCSYFEYGNRGPGANTKRRVRWKGVRVIGRNEAMQFTAGPFLLGRAWLPATGGPYLLGLKN
Homology
BLAST of Cmc09g0251271 vs. NCBI nr
Match: KAA0050276.1 (pectinesterase-like [Cucumis melo var. makuwa] >TYJ98131.1 pectinesterase-like [Cucumis melo var. makuwa])

HSP 1 Score: 1127.5 bits (2915), Expect = 0.0e+00
Identity = 565/565 (100.00%), Postives = 565/565 (100.00%), Query Frame = 0

Query: 1   MVAKVVMAVTSLLVVGVVLAVFAMVNKNHSSDETADMSPKMKAVSSICSNTDYQQECQTT 60
           MVAKVVMAVTSLLVVGVVLAVFAMVNKNHSSDETADMSPKMKAVSSICSNTDYQQECQTT
Sbjct: 1   MVAKVVMAVTSLLVVGVVLAVFAMVNKNHSSDETADMSPKMKAVSSICSNTDYQQECQTT 60

Query: 61  LGNVGHNSSVDDPKEFLKAAITATIEEMKKGYNLTDNLMVEAANNASIKMTVDDCRDLLQ 120
           LGNVGHNSSVDDPKEFLKAAITATIEEMKKGYNLTDNLMVEAANNASIKMTVDDCRDLLQ
Sbjct: 61  LGNVGHNSSVDDPKEFLKAAITATIEEMKKGYNLTDNLMVEAANNASIKMTVDDCRDLLQ 120

Query: 121 SAIDELHASYSTVGDPDLHTNEDRVADIKNWLTAVISYQQSCLDGLEEFDPQLKQKMQGG 180
           SAIDELHASYSTVGDPDLHTNEDRVADIKNWLTAVISYQQSCLDGLEEFDPQLKQKMQGG
Sbjct: 121 SAIDELHASYSTVGDPDLHTNEDRVADIKNWLTAVISYQQSCLDGLEEFDPQLKQKMQGG 180

Query: 181 LDVAAKLTSNALAIVDAVSDILKSLGLQLKVQPSGRRLLGTTEVDNDGYPTWLTGADRKL 240
           LDVAAKLTSNALAIVDAVSDILKSLGLQLKVQPSGRRLLGTTEVDNDGYPTWLTGADRKL
Sbjct: 181 LDVAAKLTSNALAIVDAVSDILKSLGLQLKVQPSGRRLLGTTEVDNDGYPTWLTGADRKL 240

Query: 241 LAAKRGGVRVKPNAVVAKDGSGQYKTIAAALAAYPKALKGRYVIYVKAGIYNEYIIVTKD 300
           LAAKRGGVRVKPNAVVAKDGSGQYKTIAAALAAYPKALKGRYVIYVKAGIYNEYIIVTKD
Sbjct: 241 LAAKRGGVRVKPNAVVAKDGSGQYKTIAAALAAYPKALKGRYVIYVKAGIYNEYIIVTKD 300

Query: 301 MKNVFMYGDGPRKTIVTGRKSNRDGINTQNTATFAAIGEGFLCKSMGFTNTAGPEGHQAV 360
           MKNVFMYGDGPRKTIVTGRKSNRDGINTQNTATFAAIGEGFLCKSMGFTNTAGPEGHQAV
Sbjct: 301 MKNVFMYGDGPRKTIVTGRKSNRDGINTQNTATFAAIGEGFLCKSMGFTNTAGPEGHQAV 360

Query: 361 ALRVQSDRSAFFNCRMDGYQDTLYVQTHRQFYRNCVVSGTVDFIFGDSNTIIQNSLIIVR 420
           ALRVQSDRSAFFNCRMDGYQDTLYVQTHRQFYRNCVVSGTVDFIFGDSNTIIQNSLIIVR
Sbjct: 361 ALRVQSDRSAFFNCRMDGYQDTLYVQTHRQFYRNCVVSGTVDFIFGDSNTIIQNSLIIVR 420

Query: 421 RPMDNQQNTVTANGRADKKEISGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKEYARTVI 480
           RPMDNQQNTVTANGRADKKEISGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKEYARTVI
Sbjct: 421 RPMDNQQNTVTANGRADKKEISGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKEYARTVI 480

Query: 481 METTLGDFIQPAGYMPWSGDFALATCSYFEYGNRGPGANTKRRVRWKGVRVIGRNEAMQF 540
           METTLGDFIQPAGYMPWSGDFALATCSYFEYGNRGPGANTKRRVRWKGVRVIGRNEAMQF
Sbjct: 481 METTLGDFIQPAGYMPWSGDFALATCSYFEYGNRGPGANTKRRVRWKGVRVIGRNEAMQF 540

Query: 541 TAGPFLLGRAWLPATGGPYLLGLKN 566
           TAGPFLLGRAWLPATGGPYLLGLKN
Sbjct: 541 TAGPFLLGRAWLPATGGPYLLGLKN 565

BLAST of Cmc09g0251271 vs. NCBI nr
Match: XP_008466382.2 (PREDICTED: pectinesterase-like [Cucumis melo])

HSP 1 Score: 1118.6 bits (2892), Expect = 0.0e+00
Identity = 558/559 (99.82%), Postives = 559/559 (100.00%), Query Frame = 0

Query: 7   MAVTSLLVVGVVLAVFAMVNKNHSSDETADMSPKMKAVSSICSNTDYQQECQTTLGNVGH 66
           MAVTSLLVVGVVLAVFAMVNKNHSSDETADMSPKMKAVSSICSNTDYQQECQTTLGNVGH
Sbjct: 1   MAVTSLLVVGVVLAVFAMVNKNHSSDETADMSPKMKAVSSICSNTDYQQECQTTLGNVGH 60

Query: 67  NSSVDDPKEFLKAAITATIEEMKKGYNLTDNLMVEAANNASIKMTVDDCRDLLQSAIDEL 126
           NSSVDDPKEFLKAAITATIEEMKKGYNLTDNLMVEAANNASIKMTVDDCRDLLQSAIDEL
Sbjct: 61  NSSVDDPKEFLKAAITATIEEMKKGYNLTDNLMVEAANNASIKMTVDDCRDLLQSAIDEL 120

Query: 127 HASYSTVGDPDLHTNEDRVADIKNWLTAVISYQQSCLDGLEEFDPQLKQKMQGGLDVAAK 186
           HASYSTVGDPDLHTNEDRVADIKNWLTAVISYQQSCLDGLEEFDPQLKQKMQGGLDVAAK
Sbjct: 121 HASYSTVGDPDLHTNEDRVADIKNWLTAVISYQQSCLDGLEEFDPQLKQKMQGGLDVAAK 180

Query: 187 LTSNALAIVDAVSDILKSLGLQLKVQPSGRRLLGTTEVDNDGYPTWLTGADRKLLAAKRG 246
           LTSNALAIVDAVSDILKSLGLQLKVQPSGRRLLGTTEVDNDG+PTWLTGADRKLLAAKRG
Sbjct: 181 LTSNALAIVDAVSDILKSLGLQLKVQPSGRRLLGTTEVDNDGFPTWLTGADRKLLAAKRG 240

Query: 247 GVRVKPNAVVAKDGSGQYKTIAAALAAYPKALKGRYVIYVKAGIYNEYIIVTKDMKNVFM 306
           GVRVKPNAVVAKDGSGQYKTIAAALAAYPKALKGRYVIYVKAGIYNEYIIVTKDMKNVFM
Sbjct: 241 GVRVKPNAVVAKDGSGQYKTIAAALAAYPKALKGRYVIYVKAGIYNEYIIVTKDMKNVFM 300

Query: 307 YGDGPRKTIVTGRKSNRDGINTQNTATFAAIGEGFLCKSMGFTNTAGPEGHQAVALRVQS 366
           YGDGPRKTIVTGRKSNRDGINTQNTATFAAIGEGFLCKSMGFTNTAGPEGHQAVALRVQS
Sbjct: 301 YGDGPRKTIVTGRKSNRDGINTQNTATFAAIGEGFLCKSMGFTNTAGPEGHQAVALRVQS 360

Query: 367 DRSAFFNCRMDGYQDTLYVQTHRQFYRNCVVSGTVDFIFGDSNTIIQNSLIIVRRPMDNQ 426
           DRSAFFNCRMDGYQDTLYVQTHRQFYRNCVVSGTVDFIFGDSNTIIQNSLIIVRRPMDNQ
Sbjct: 361 DRSAFFNCRMDGYQDTLYVQTHRQFYRNCVVSGTVDFIFGDSNTIIQNSLIIVRRPMDNQ 420

Query: 427 QNTVTANGRADKKEISGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKEYARTVIMETTLG 486
           QNTVTANGRADKKEISGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKEYARTVIMETTLG
Sbjct: 421 QNTVTANGRADKKEISGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKEYARTVIMETTLG 480

Query: 487 DFIQPAGYMPWSGDFALATCSYFEYGNRGPGANTKRRVRWKGVRVIGRNEAMQFTAGPFL 546
           DFIQPAGYMPWSGDFALATCSYFEYGNRGPGANTKRRVRWKGVRVIGRNEAMQFTAGPFL
Sbjct: 481 DFIQPAGYMPWSGDFALATCSYFEYGNRGPGANTKRRVRWKGVRVIGRNEAMQFTAGPFL 540

Query: 547 LGRAWLPATGGPYLLGLKN 566
           LGRAWLPATGGPYLLGLKN
Sbjct: 541 LGRAWLPATGGPYLLGLKN 559

BLAST of Cmc09g0251271 vs. NCBI nr
Match: KGN49779.1 (hypothetical protein Csa_017783 [Cucumis sativus])

HSP 1 Score: 1047.7 bits (2708), Expect = 3.5e-302
Identity = 519/566 (91.70%), Postives = 547/566 (96.64%), Query Frame = 0

Query: 1   MVAKVVMA-VTSLLVVGVVLAVFAMVNKNHSSDETADMSPKMKAVSSICSNTDYQQECQT 60
           MV KVVM+ ++ +LVVGV LAV A+VNK+ SSDETA++SPKMKAV+SICSN DYQ ECQT
Sbjct: 1   MVGKVVMSGISLILVVGVALAVVAIVNKSDSSDETANLSPKMKAVASICSNADYQPECQT 60

Query: 61  TLGNVGHNSSVDDPKEFLKAAITATIEEMKKGYNLTDNLMVEAANNASIKMTVDDCRDLL 120
           TLG+VG+NSSV+DPKEF+KAAIT+TIEEMKKGYNLTDNLMVEAANNA+IKM+VDDC+DLL
Sbjct: 61  TLGSVGNNSSVEDPKEFIKAAITSTIEEMKKGYNLTDNLMVEAANNATIKMSVDDCKDLL 120

Query: 121 QSAIDELHASYSTVGDPDLHTNEDRVADIKNWLTAVISYQQSCLDGLEEFDPQLKQKMQG 180
           QSAIDELHASYSTVGDPDLHTNEDR+ADIKNWLTAVISYQQSCLDGLEEFDPQLKQKMQ 
Sbjct: 121 QSAIDELHASYSTVGDPDLHTNEDRIADIKNWLTAVISYQQSCLDGLEEFDPQLKQKMQD 180

Query: 181 GLDVAAKLTSNALAIVDAVSDILKSLGLQLKVQPSGRRLLGTTEVDNDGYPTWLTGADRK 240
           GLDVA KLTSNALAIV AVSDILK LGLQLKVQPSGRRLLGTTEVD+D +PTWLTG+DRK
Sbjct: 181 GLDVAGKLTSNALAIVGAVSDILKELGLQLKVQPSGRRLLGTTEVDSDSFPTWLTGSDRK 240

Query: 241 LLAAKRGGVRVKPNAVVAKDGSGQYKTIAAALAAYPKALKGRYVIYVKAGIYNEYIIVTK 300
           LLAAKRGGVRVKPN VVAKDGSGQYKTI AALAAYPKALKGRYVIYVKAG+YNE IIVTK
Sbjct: 241 LLAAKRGGVRVKPNVVVAKDGSGQYKTIGAALAAYPKALKGRYVIYVKAGVYNEPIIVTK 300

Query: 301 DMKNVFMYGDGPRKTIVTGRKSNRDGINTQNTATFAAIGEGFLCKSMGFTNTAGPEGHQA 360
           DMKN+FMYGDGPRKTIVTGRKSNRDGI TQNTA+FAAIGEGFLCKSMGFTNTAGPEGHQA
Sbjct: 301 DMKNIFMYGDGPRKTIVTGRKSNRDGITTQNTASFAAIGEGFLCKSMGFTNTAGPEGHQA 360

Query: 361 VALRVQSDRSAFFNCRMDGYQDTLYVQTHRQFYRNCVVSGTVDFIFGDSNTIIQNSLIIV 420
           VALRVQSDRSAFFNCRMDGYQDTLYVQTHRQFYRNCV+SGTVDFIFGDSNTIIQNSLIIV
Sbjct: 361 VALRVQSDRSAFFNCRMDGYQDTLYVQTHRQFYRNCVISGTVDFIFGDSNTIIQNSLIIV 420

Query: 421 RRPMDNQQNTVTANGRADKKEISGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKEYARTV 480
           RRPMDNQQNTVTANGRADKKEISGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKEYARTV
Sbjct: 421 RRPMDNQQNTVTANGRADKKEISGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKEYARTV 480

Query: 481 IMETTLGDFIQPAGYMPWSGDFALATCSYFEYGNRGPGANTKRRVRWKGVRVIGRNEAMQ 540
           IME+TLGDFIQPAGYMPWSG+FALATCSYFEYGNRGPGANT RRVRWKGVRVIGRNEAMQ
Sbjct: 481 IMESTLGDFIQPAGYMPWSGNFALATCSYFEYGNRGPGANTNRRVRWKGVRVIGRNEAMQ 540

Query: 541 FTAGPFLLGRAWLPATGGPYLLGLKN 566
           FTAGPFLLG+AWLP TGGPYLLGLKN
Sbjct: 541 FTAGPFLLGKAWLPGTGGPYLLGLKN 566

BLAST of Cmc09g0251271 vs. NCBI nr
Match: XP_038875879.1 (LOW QUALITY PROTEIN: pectinesterase-like [Benincasa hispida])

HSP 1 Score: 989.9 bits (2558), Expect = 8.7e-285
Identity = 491/566 (86.75%), Postives = 531/566 (93.82%), Query Frame = 0

Query: 1   MVAKVVMA-VTSLLVVGVVLAVFAMVNKNHSSDETADMSPKMKAVSSICSNTDYQQECQT 60
           MV KVV++ ++ +LVVGV LAV AMV KN++SD     SPKMKAVS+ICS TDYQQECQ 
Sbjct: 1   MVGKVVVSGISLILVVGVALAVVAMVTKNNNSDVDLS-SPKMKAVSTICSTTDYQQECQN 60

Query: 61  TLGNVGHNSSVDDPKEFLKAAITATIEEMKKGYNLTDNLMVEAANNASIKMTVDDCRDLL 120
           TL NV  N+SVDDPKE+ KAA+ ATI+E+ KGYNLT +LMVEAANNASIKM+VDDC+DLL
Sbjct: 61  TLTNVAQNASVDDPKEYAKAAVLATIQEITKGYNLTADLMVEAANNASIKMSVDDCKDLL 120

Query: 121 QSAIDELHASYSTVGDPDLHTNEDRVADIKNWLTAVISYQQSCLDGLEEFDPQLKQKMQG 180
           QSAIDELHASYSTVGDPDLHTNEDRVADIKNWLTAVISYQQSCLDGLEEFDPQLKQKMQ 
Sbjct: 121 QSAIDELHASYSTVGDPDLHTNEDRVADIKNWLTAVISYQQSCLDGLEEFDPQLKQKMQD 180

Query: 181 GLDVAAKLTSNALAIVDAVSDILKSLGLQLKVQPSGRRLLGTTEVDNDGYPTWLTGADRK 240
           GLDVA KLTSNALAIV AVSDILK+ GLQLKVQPSGRRLL TTE+ +DG+PTW++GADRK
Sbjct: 181 GLDVAGKLTSNALAIVGAVSDILKNFGLQLKVQPSGRRLLETTEIGSDGFPTWMSGADRK 240

Query: 241 LLAAKRGGVRVKPNAVVAKDGSGQYKTIAAALAAYPKALKGRYVIYVKAGIYNEYIIVTK 300
           LLA++ GG RVKPNAVVAKDGSGQYK+I AALAAYPKAL+GRYVIYVKAGIY+EYI VTK
Sbjct: 241 LLASRGGGARVKPNAVVAKDGSGQYKSIGAALAAYPKALRGRYVIYVKAGIYDEYITVTK 300

Query: 301 DMKNVFMYGDGPRKTIVTGRKSNRDGINTQNTATFAAIGEGFLCKSMGFTNTAGPEGHQA 360
           DMKNVFMYGDGPRKTIVTGRK+NRDGI TQNTA+F+AIGEGFLCKSMGF NTAGPEGHQA
Sbjct: 301 DMKNVFMYGDGPRKTIVTGRKNNRDGITTQNTASFSAIGEGFLCKSMGFRNTAGPEGHQA 360

Query: 361 VALRVQSDRSAFFNCRMDGYQDTLYVQTHRQFYRNCVVSGTVDFIFGDSNTIIQNSLIIV 420
           VALRVQSDRSAF+NCRMDGYQDTLYVQTHRQFYRNCV+SGTVDFIFGDS+TIIQNSLIIV
Sbjct: 361 VALRVQSDRSAFYNCRMDGYQDTLYVQTHRQFYRNCVISGTVDFIFGDSSTIIQNSLIIV 420

Query: 421 RRPMDNQQNTVTANGRADKKEISGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKEYARTV 480
           RRPMDNQ+NTVTANGRADKKEISGLVIHNCRIVPEQKLFAERFKIPTFLGRPWK+YARTV
Sbjct: 421 RRPMDNQKNTVTANGRADKKEISGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKQYARTV 480

Query: 481 IMETTLGDFIQPAGYMPWSGDFALATCSYFEYGNRGPGANTKRRVRWKGVRVIGRNEAMQ 540
           IMETTLGDFIQP GYMPWSG+FAL TCSYFEYGNRGPGANT RRVRWKGVRVIGRNEA+Q
Sbjct: 481 IMETTLGDFIQPDGYMPWSGNFALETCSYFEYGNRGPGANTNRRVRWKGVRVIGRNEALQ 540

Query: 541 FTAGPFLLGRAWLPATGGPYLLGLKN 566
           +TAGPFL+GRAWLPATGGPYLLGLK+
Sbjct: 541 YTAGPFLMGRAWLPATGGPYLLGLKH 565

BLAST of Cmc09g0251271 vs. NCBI nr
Match: XP_008466374.1 (PREDICTED: pectinesterase-like [Cucumis melo])

HSP 1 Score: 958.4 bits (2476), Expect = 2.8e-275
Identity = 474/567 (83.60%), Postives = 513/567 (90.48%), Query Frame = 0

Query: 1   MVAKVVM-AVTSLLVVGVVLAVFAMVNKNHSSD-ETADMSPKMKAVSSICSNTDYQQECQ 60
           MV KV++  +T +LVVGV LAV A+V+ N SSD  T D+SPKMKAVS+IC+N +YQQECQ
Sbjct: 1   MVNKVILPGITLILVVGVALAVVAIVHSNKSSDAATEDLSPKMKAVSTICANANYQQECQ 60

Query: 61  TTLGNVGHNSSVDDPKEFLKAAITATIEEMKKGYNLTDNLMVEAANNASIKMTVDDCRDL 120
            TL N  HN+S DDPKE+++AAI +TIEE+KKGYNLTD  M+EAANN SIKM V+DCRDL
Sbjct: 61  NTLSNAAHNASSDDPKEYVRAAILSTIEEVKKGYNLTDGFMIEAANNRSIKMGVEDCRDL 120

Query: 121 LQSAIDELHASYSTVGDPDLHTNEDRVADIKNWLTAVISYQQSCLDGLEEFDPQLKQKMQ 180
           LQ AID+L ASYSTVG+PDLHTN DRVADIKNWLT+VISYQQSCLDGLEEFDPQLKQKMQ
Sbjct: 121 LQFAIDQLQASYSTVGEPDLHTNADRVADIKNWLTSVISYQQSCLDGLEEFDPQLKQKMQ 180

Query: 181 GGLDVAAKLTSNALAIVDAVSDILKSLGLQLKVQPSGRRLLGTTEVDNDGYPTWLTGADR 240
            GL  A KLTSNALAIVDAVSDIL S GLQLK QPSGRRLLGTTEV  DGYPTWLTGADR
Sbjct: 181 DGLSDAGKLTSNALAIVDAVSDILASFGLQLKAQPSGRRLLGTTEVGKDGYPTWLTGADR 240

Query: 241 KLLAAKRGGVRVKPNAVVAKDGSGQYKTIAAALAAYPKALKGRYVIYVKAGIYNEYIIVT 300
           KLLA++ GG +VKPNAVVAKDGSGQ+KTI+AALAAYPKAL+GRYVIYVKAGIYNEY+ +T
Sbjct: 241 KLLASRGGGAKVKPNAVVAKDGSGQFKTISAALAAYPKALRGRYVIYVKAGIYNEYVHIT 300

Query: 301 KDMKNVFMYGDGPRKTIVTGRKSNRDGINTQNTATFAAIGEGFLCKSMGFTNTAGPEGHQ 360
           KDMKN+FMYGDGP+KTIVTG KSNR G  TQ+TATF AIGEGFLCKSMGF NTAGPEGHQ
Sbjct: 301 KDMKNIFMYGDGPKKTIVTGNKSNRGGFTTQDTATFIAIGEGFLCKSMGFQNTAGPEGHQ 360

Query: 361 AVALRVQSDRSAFFNCRMDGYQDTLYVQTHRQFYRNCVVSGTVDFIFGDSNTIIQNSLII 420
           AVALRVQSDRSAFFNCRMDGYQDTLYVQT RQFYRNCV+SGTVDFIFGDS TIIQNSLII
Sbjct: 361 AVALRVQSDRSAFFNCRMDGYQDTLYVQTQRQFYRNCVISGTVDFIFGDSTTIIQNSLII 420

Query: 421 VRRPMDNQQNTVTANGRADKKEISGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKEYART 480
           VRRPMDNQQNTVTANGRAD KE+SGLVIHNCRIVPEQKLFAERFKIPTFLGRPWK+YART
Sbjct: 421 VRRPMDNQQNTVTANGRADPKEVSGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKQYART 480

Query: 481 VIMETTLGDFIQPAGYMPWSGDFALATCSYFEYGNRGPGANTKRRVRWKGVRVIGRNEAM 540
           VIME+TLGDFIQP GYMPWSG+FAL TC Y EYGNRGPGA T RRVRWKGV+VIGRNEAM
Sbjct: 481 VIMESTLGDFIQPVGYMPWSGNFALETCLYLEYGNRGPGAVTNRRVRWKGVKVIGRNEAM 540

Query: 541 QFTAGPFLLGRAWLPATGGPYLLGLKN 566
           QFTAGPFL GRAWLP TGGPYLLGLK+
Sbjct: 541 QFTAGPFLQGRAWLPTTGGPYLLGLKS 567

BLAST of Cmc09g0251271 vs. ExPASy Swiss-Prot
Match: Q8GX86 (Probable pectinesterase/pectinesterase inhibitor 21 OS=Arabidopsis thaliana OX=3702 GN=PME21 PE=2 SV=2)

HSP 1 Score: 454.9 bits (1169), Expect = 1.3e-126
Identity = 229/562 (40.75%), Postives = 347/562 (61.74%), Query Frame = 0

Query: 5   VVMAVTSLLVVGVVLAVFAMVNKN-HSSDE--TADMSPKMKAVSSICSNTDYQQECQTTL 64
           +V+ ++S+L++ +V+AV   V+ N H  D    A+++  +KAV  +C+ TDY++ C+ TL
Sbjct: 16  IVITISSVLLISMVVAVTVGVSLNKHDGDSKGKAEVNASVKAVKDVCAPTDYRKTCEDTL 75

Query: 65  GNVGHNSSVDDPKEFLKAAITATIEEMKKGYNLTDNLMVEAANNASIKMTVDDCRDLLQS 124
              G N++  DP E +K A   T++++      +  +M E   ++  +M +D C++L+  
Sbjct: 76  IKNGKNTT--DPMELVKTAFNVTMKQITDAAKKSQTIM-ELQKDSRTRMALDQCKELMDY 135

Query: 125 AIDELHASYSTVGDPDLHTNEDRVADIKNWLTAVISYQQSCLDGLEEFDPQLKQKMQGGL 184
           A+DEL  S+  +G  + H  ++ + +++ WL+A IS++++CL+G +       + M+  L
Sbjct: 136 ALDELSNSFEELGKFEFHLLDEALINLRIWLSAAISHEETCLEGFQGTQGNAGETMKKAL 195

Query: 185 DVAAKLTSNALAIVDAVSDILKSLGLQLKVQPSGRRLLGTTEVDNDGYPTWLTGADRKLL 244
             A +LT N LAI+  +S+ +  + +      + RRLL       +G+P+W+    RKLL
Sbjct: 196 KTAIELTHNGLAIISEMSNFVGQMQIP---GLNSRRLLA------EGFPSWVDQRGRKLL 255

Query: 245 AAKRGGVRVKPNAVVAKDGSGQYKTIAAALAAYPKALKGRYVIYVKAGIYNEYIIVTKDM 304
            A      VKP+ VVA+DGSGQYKTI  AL   PK     +V+++KAG+Y EY+ V K M
Sbjct: 256 QAAAAYSDVKPDIVVAQDGSGQYKTINEALQFVPKKRNTTFVVHIKAGLYKEYVQVNKTM 315

Query: 305 KNVFMYGDGPRKTIVTGRKSNRDGINTQNTATFAAIGEGFLCKSMGFTNTAGPEGHQAVA 364
            ++   GDGP KTI++G K+ +DGI T  TAT A +G  F+ K++GF NTAG   HQAVA
Sbjct: 316 SHLVFIGDGPDKTIISGNKNYKDGITTYRTATVAIVGNYFIAKNIGFENTAGAIKHQAVA 375

Query: 365 LRVQSDRSAFFNCRMDGYQDTLYVQTHRQFYRNCVVSGTVDFIFGDSNTIIQNSLIIVRR 424
           +RVQSD S FFNCR DGYQDTLY  +HRQF+R+C +SGT+DF+FGD+  + QN  ++VR+
Sbjct: 376 VRVQSDESIFFNCRFDGYQDTLYTHSHRQFFRDCTISGTIDFLFGDAAAVFQNCTLLVRK 435

Query: 425 PMDNQQNTVTANGRADKKEISGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKEYARTVIM 484
           P+ NQ   +TA+GR D +E +G V   C I  E    A +     +LGRPWKEY+RT+IM
Sbjct: 436 PLPNQACPITAHGRKDPRESTGFVFQGCTIAGEPDYLAVKETSKAYLGRPWKEYSRTIIM 495

Query: 485 ETTLGDFIQPAGYMPWSGDFALATCSYFEYGNRGPGANTKRRVRWKGVRVIGRNEAMQFT 544
            T + DF+QP G+ PW GDF L T  Y E  N GPG+    RV W G++ +   + ++FT
Sbjct: 496 NTFIPDFVQPQGWQPWLGDFGLKTLFYSEVQNTGPGSALANRVTWAGIKTLSEEDILKFT 555

Query: 545 AGPFLLGRAWLPATGGPYLLGL 564
              ++ G  W+P  G PY  GL
Sbjct: 556 PAQYIQGDDWIPGKGVPYTTGL 565

BLAST of Cmc09g0251271 vs. ExPASy Swiss-Prot
Match: Q9SMY6 (Putative pectinesterase/pectinesterase inhibitor 45 OS=Arabidopsis thaliana OX=3702 GN=PME45 PE=2 SV=1)

HSP 1 Score: 452.6 bits (1163), Expect = 6.6e-126
Identity = 242/536 (45.15%), Postives = 338/536 (63.06%), Query Frame = 0

Query: 30  SSDETADMSPKMKAVSSICSNTDYQQECQTTLGN-VGHNSSVDDPKEFLKAAITATIEEM 89
           S+ +T       K + ++C++T Y+  CQ TL N    ++   DP+  LK+AI A  +++
Sbjct: 81  SAAQTVKAGQVDKIIQTLCNSTLYKPTCQNTLKNETKKDTPQTDPRSLLKSAIVAVNDDL 140

Query: 90  KKGYNLTDNLMVEAANNASIKMTVDDCRDLLQSAIDELHASYSTVGDPDLHTNEDRVADI 149
            + +    +L  E   N   K  +  C+ L+  A +EL  S   + D +++     V D+
Sbjct: 141 DQVFKRVLSLKTE---NKDDKDAIAQCKLLVDEAKEELGTSMKRINDSEVNNFAKIVPDL 200

Query: 150 KNWLTAVISYQQSCLDGLEEFDPQLKQKMQGGLDVAAKLTSNALAIVDAVSDILKSLGLQ 209
            +WL+AV+SYQ++C+DG EE   +LK +++   + +  LTSN+LA++ ++   L S+   
Sbjct: 201 DSWLSAVMSYQETCVDGFEE--GKLKTEIRKNFNSSQVLTSNSLAMIKSLDGYLSSVP-- 260

Query: 210 LKVQPSGRRLLGTTEVDNDGYPTWLTGADRKLLAAKRGGVRVKPNAVVAKDGSGQYKTIA 269
            KV+        ++  + D   +WL+  +R++L A      +KPNA VAKDGSG + TI 
Sbjct: 261 -KVKTRLLLEARSSAKETDHITSWLSNKERRMLKAV-DVKALKPNATVAKDGSGNFTTIN 320

Query: 270 AALAAYPKALKGRYVIYVKAGIYNEYIIVTKDMKNVFMYGDGPRKTIVTGRKSNRDGINT 329
           AAL A P   +GRY IY+K GIY+E +I+ K   NV M GDG +KTIVTG KS+   I T
Sbjct: 321 AALKAMPAKYQGRYTIYIKHGIYDESVIIDKKKPNVTMVGDGSQKTIVTGNKSHAKKIRT 380

Query: 330 QNTATFAAIGEGFLCKSMGFTNTAGPEGHQAVALRVQSDRSAFFNCRMDGYQDTLYVQTH 389
             TATF A GEGF+ +SMGF NTAGPEGHQAVA+RVQSDRS F NCR +GYQDTLY  TH
Sbjct: 381 FLTATFVAQGEGFMAQSMGFRNTAGPEGHQAVAIRVQSDRSVFLNCRFEGYQDTLYAYTH 440

Query: 390 RQFYRNCVVSGTVDFIFGDSNTIIQNSLIIVRRPMDNQQNTVTANGRADKKEISGLVIHN 449
           RQ+YR+CV+ GTVDFIFGD+  I QN  I +R+ +  Q+NTVTA GR DK + +G VIHN
Sbjct: 441 RQYYRSCVIIGTVDFIFGDAAAIFQNCDIFIRKGLPGQKNTVTAQGRVDKFQTTGFVIHN 500

Query: 450 CRIVPEQKLFAERFKIPTFLGRPWKEYARTVIMETTLGDFIQPAGYMPW-SGDFALATCS 509
           C + P + L   + +  ++LGRPWK ++RTV+ME+T+ D I P G++ W   DFA+ T S
Sbjct: 501 CTVAPNEDLKPVKAQFKSYLGRPWKPHSRTVVMESTIEDVIDPVGWLRWQETDFAIDTLS 560

Query: 510 YFEYGNRGPGANTKRRVRWKGVRVIGRNEAMQFTAGPFLLGRAWLPATGGPYLLGL 564
           Y EY N GP   T  RV+W G RV+ + EAM+FT GPFL G  W+ A G P  LGL
Sbjct: 561 YAEYKNDGPSGATAARVKWPGFRVLNKEEAMKFTVGPFLQGE-WIQAIGSPVKLGL 606

BLAST of Cmc09g0251271 vs. ExPASy Swiss-Prot
Match: Q7Y201 (Probable pectinesterase/pectinesterase inhibitor 13 OS=Arabidopsis thaliana OX=3702 GN=PME13 PE=2 SV=2)

HSP 1 Score: 448.0 bits (1151), Expect = 1.6e-124
Identity = 238/524 (45.42%), Postives = 339/524 (64.69%), Query Frame = 0

Query: 42  KAVSSICSNTDYQQECQTTLGN-VGHNSSVDDPKEFLKAAITATIEEMKKGYNLTDNLMV 101
           K + ++CS+T Y Q C+ TL N      ++D+P  FLK+AI A  E++     + + ++ 
Sbjct: 107 KIIQTLCSSTLYMQICEKTLKNRTDKGFALDNPTTFLKSAIEAVNEDLDL---VLEKVLS 166

Query: 102 EAANNASIKMTVDDCRDLLQSAIDELHASYSTVGDPDLHTNEDRVADIKNWLTAVISYQQ 161
               N   K  ++ C+ L++ A +E  AS + +   ++++ E  V D+++WL+AV+SYQ+
Sbjct: 167 LKTENQDDKDAIEQCKLLVEDAKEETVASLNKINVTEVNSFEKVVPDLESWLSAVMSYQE 226

Query: 162 SCLDGLEEFDPQLKQKMQGGLDVAAKLTSNALAIVDAVSDILKSLGLQLKVQPSGRRLLG 221
           +CLDG EE    LK +++  ++ +  LTSN+LA++   ++ L  +   +KV    R LL 
Sbjct: 227 TCLDGFEE--GNLKSEVKTSVNSSQVLTSNSLALIKTFTENLSPV---MKVVE--RHLL- 286

Query: 222 TTEVDNDGYPTWLTGADRKLLAAKRGGVRVKPNAVVAKDGSGQYKTIAAALAAYPKALKG 281
                 D  P+W++  DR++L A      +KPNA VAKDGSG + TI  AL A P+  +G
Sbjct: 287 ------DDIPSWVSNDDRRMLRAV-DVKALKPNATVAKDGSGDFTTINDALRAMPEKYEG 346

Query: 282 RYVIYVKAGIYNEYIIVTKDMKNVFMYGDGPRKTIVTGRKSNRDGINTQNTATFAAIGEG 341
           RY+IYVK GIY+EY+ V K   N+ M GDG +KTIVTG KS+   I T  TATF A GEG
Sbjct: 347 RYIIYVKQGIYDEYVTVDKKKANLTMVGDGSQKTIVTGNKSHAKKIRTFLTATFVAQGEG 406

Query: 342 FLCKSMGFTNTAGPEGHQAVALRVQSDRSAFFNCRMDGYQDTLYVQTHRQFYRNCVVSGT 401
           F+ +SMGF NTAGPEGHQAVA+RVQSDRS F NCR +GYQDTLY  THRQ+YR+CV+ GT
Sbjct: 407 FMAQSMGFRNTAGPEGHQAVAIRVQSDRSIFLNCRFEGYQDTLYAYTHRQYYRSCVIVGT 466

Query: 402 VDFIFGDSNTIIQNSLIIVRRPMDNQQNTVTANGRADKKEISGLVIHNCRIVPEQKLFAE 461
           +DFIFGD+  I QN  I +R+ +  Q+NTVTA GR DK + +G V+HNC+I   + L   
Sbjct: 467 IDFIFGDAAAIFQNCNIFIRKGLPGQKNTVTAQGRVDKFQTTGFVVHNCKIAANEDLKPV 526

Query: 462 RFKIPTFLGRPWKEYARTVIMETTLGDFIQPAGYMPW-SGDFALATCSYFEYGNRGPGAN 521
           + +  ++LGRPWK Y+RT+IME+ + + I P G++ W   DFA+ T  Y EY N+G   +
Sbjct: 527 KEEYKSYLGRPWKNYSRTIIMESKIENVIDPVGWLRWQETDFAIDTLYYAEYNNKGSSGD 586

Query: 522 TKRRVRWKGVRVIGRNEAMQFTAGPFLLGRAWLPATGGPYLLGL 564
           T  RV+W G +VI + EA+ +T GPFL G  W+ A+G P  LGL
Sbjct: 587 TTSRVKWPGFKVINKEEALNYTVGPFLQG-DWISASGSPVKLGL 611

BLAST of Cmc09g0251271 vs. ExPASy Swiss-Prot
Match: Q3E8Z8 (Putative pectinesterase/pectinesterase inhibitor 28 OS=Arabidopsis thaliana OX=3702 GN=PME28 PE=2 SV=1)

HSP 1 Score: 446.4 bits (1147), Expect = 4.7e-124
Identity = 226/560 (40.36%), Postives = 349/560 (62.32%), Query Frame = 0

Query: 5   VVMAVTSLLVVGVVLAVFAMVNKNHSSDE-TADMSPKMKAVSSICSNTDYQQECQTTLGN 64
           V+++++S+L++ +V+AV   V+ N S +    +++  +KA+  +C+ TDY++ C+ TL  
Sbjct: 17  VIISISSVLLISMVVAVTIGVSVNKSDNAGDEEITTSVKAIKDVCAPTDYKETCEDTLRK 76

Query: 65  VGHNSSVDDPKEFLKAAITATIEEMKKGYNLTDNLMVEAANNASIKMTVDDCRDLLQSAI 124
              ++S  DP E +K A  AT++++      +   M+E   +   KM +D C++L+  AI
Sbjct: 77  DAKDTS--DPLELVKTAFNATMKQISDVAKKSQT-MIELQKDPRAKMALDQCKELMDYAI 136

Query: 125 DELHASYSTVGDPDLHTNEDRVADIKNWLTAVISYQQSCLDGLEEFDPQLKQKMQGGLDV 184
            EL  S+  +G  + H  ++ +  ++ WL+A IS++Q+CLDG +       + ++  L  
Sbjct: 137 GELSKSFEELGKFEFHKVDEALVKLRIWLSATISHEQTCLDGFQGTQGNAGETIKKALKT 196

Query: 185 AAKLTSNALAIVDAVSDILKSLGLQLKVQPSGRRLLGTTEVDNDGYPTWLTGADRKLLAA 244
           A +LT N LA+V  +S+ L  + +    + + RRLL      +  +P+W+    R+LL A
Sbjct: 197 AVQLTHNGLAMVTEMSNYLGQMQIP---EMNSRRLL------SQEFPSWMDARARRLLNA 256

Query: 245 KRGGVRVKPNAVVAKDGSGQYKTIAAALAAYPKALKGRYVIYVKAGIYNEYIIVTKDMKN 304
                 VKP+ VVA+DGSGQYKTI  AL   PK     +V+++K GIY EY+ V + M +
Sbjct: 257 PMS--EVKPDIVVAQDGSGQYKTINEALNFVPKKKNTTFVVHIKEGIYKEYVQVNRSMTH 316

Query: 305 VFMYGDGPRKTIVTGRKSNRDGINTQNTATFAAIGEGFLCKSMGFTNTAGPEGHQAVALR 364
           +   GDGP KT+++G KS +DGI T  TAT A +G+ F+ K++ F NTAG   HQAVA+R
Sbjct: 317 LVFIGDGPDKTVISGSKSYKDGITTYKTATVAIVGDHFIAKNIAFENTAGAIKHQAVAIR 376

Query: 365 VQSDRSAFFNCRMDGYQDTLYVQTHRQFYRNCVVSGTVDFIFGDSNTIIQNSLIIVRRPM 424
           V +D S F+NC+ DGYQDTLY  +HRQFYR+C +SGT+DF+FGD+  + QN  ++VR+P+
Sbjct: 377 VLADESIFYNCKFDGYQDTLYAHSHRQFYRDCTISGTIDFLFGDAAAVFQNCTLLVRKPL 436

Query: 425 DNQQNTVTANGRADKKEISGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKEYARTVIMET 484
            NQ   +TA+GR D +E +G V+  C IV E    A + +  T+LGRPWKEY+RT+IM T
Sbjct: 437 LNQACPITAHGRKDPRESTGFVLQGCTIVGEPDYLAVKEQSKTYLGRPWKEYSRTIIMNT 496

Query: 485 TLGDFIQPAGYMPWSGDFALATCSYFEYGNRGPGANTKRRVRWKGVRVIGRNEAMQFTAG 544
            + DF+ P G+ PW G+F L T  Y E  N GPGA   +RV W G++ +   E ++FT  
Sbjct: 497 FIPDFVPPEGWQPWLGEFGLNTLFYSEVQNTGPGAAITKRVTWPGIKKLSDEEILKFTPA 556

Query: 545 PFLLGRAWLPATGGPYLLGL 564
            ++ G AW+P  G PY+LGL
Sbjct: 557 QYIQGDAWIPGKGVPYILGL 562

BLAST of Cmc09g0251271 vs. ExPASy Swiss-Prot
Match: Q9FJ21 (Probable pectinesterase/pectinesterase inhibitor 58 OS=Arabidopsis thaliana OX=3702 GN=PME58 PE=2 SV=1)

HSP 1 Score: 439.1 bits (1128), Expect = 7.5e-122
Identity = 239/564 (42.38%), Postives = 339/564 (60.11%), Query Frame = 0

Query: 5   VVMAVTSLLVVGVVLAVFAMVNKNHSSDE---TADMSPKMKAVSSICSNTDYQQECQTTL 64
           ++  V + L+V +V+AV    ++N S  E      +     AV ++C+ TDY++ C  +L
Sbjct: 13  IIAGVITALLVLMVVAVGITTSRNTSHSEKIVPVQIKTATTAVEAVCAPTDYKETCVNSL 72

Query: 65  GNVGHNSSVDDPKEFLKAAITATIEEMKKGYNLTD-NLMVEAANNASIKMTVDDCRDLLQ 124
                +S+   P + +K     TI  ++         L  +AAN+   K  ++ C  L+ 
Sbjct: 73  MKASPDST--QPLDLIKLGFNVTIRSIEDSIKKASVELTAKAANDKDTKGALELCEKLMN 132

Query: 125 SAIDELHASYSTVGDPDLHTNEDRVADIKNWLTAVISYQQSCLDGLEEFDPQLKQKMQGG 184
            A D+L           +   ED V D++ WL+  I+YQQ+C+D  EE + +L Q MQ  
Sbjct: 133 DATDDLKKCLDNFDGFSIPQIEDFVEDLRVWLSGSIAYQQTCMDTFEETNSKLSQDMQKI 192

Query: 185 LDVAAKLTSNALAIVDAVSDILKSL---GLQLKVQPSGRRLLGTTEVDNDGYPTWLTGAD 244
              + +LTSN LA++  +S++L      G+   +    R+LL       DG P+W+    
Sbjct: 193 FKTSRELTSNGLAMITNISNLLGEFNVTGVTGDLGKYARKLLSA----EDGIPSWVGPNT 252

Query: 245 RKLLAAKRGGVRVKPNAVVAKDGSGQYKTIAAALAAYPKALKGRYVIYVKAGIYNEYIIV 304
           R+L+A K G   VK N VVA DGSGQYKTI  AL A PKA +  +VIY+K G+YNE + V
Sbjct: 253 RRLMATKGG---VKANVVVAHDGSGQYKTINEALNAVPKANQKPFVIYIKQGVYNEKVDV 312

Query: 305 TKDMKNVFMYGDGPRKTIVTGRKSNRDG-INTQNTATFAAIGEGFLCKSMGFTNTAGPEG 364
           TK M +V   GDGP KT +TG  +   G + T  TAT A  G+ F  K++GF NTAGPEG
Sbjct: 313 TKKMTHVTFIGDGPTKTKITGSLNYYIGKVKTYLTATVAINGDNFTAKNIGFENTAGPEG 372

Query: 365 HQAVALRVQSDRSAFFNCRMDGYQDTLYVQTHRQFYRNCVVSGTVDFIFGDSNTIIQNSL 424
           HQAVALRV +D + F+NC++DGYQDTLYV +HRQF+R+C VSGTVDFIFGD   ++QN  
Sbjct: 373 HQAVALRVSADLAVFYNCQIDGYQDTLYVHSHRQFFRDCTVSGTVDFIFGDGIVVLQNCN 432

Query: 425 IIVRRPMDNQQNTVTANGRADKKEISGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKEYA 484
           I+VR+PM +Q   +TA GR+DK+E +GLV+ NC I  E      +     +LGRPWKE++
Sbjct: 433 IVVRKPMKSQSCMITAQGRSDKRESTGLVLQNCHITGEPAYIPVKSINKAYLGRPWKEFS 492

Query: 485 RTVIMETTLGDFIQPAGYMPWSGDFALATCSYFEYGNRGPGANTKRRVRWKGVRVIGRNE 544
           RT+IM TT+ D I PAG++PW+GDFAL T  Y EY N GPG+N  +RV+W G++ +   +
Sbjct: 493 RTIIMGTTIDDVIDPAGWLPWNGDFALNTLYYAEYENNGPGSNQAQRVKWPGIKKLSPKQ 552

Query: 545 AMQFTAGPFLLGRAWLPATGGPYL 561
           A++FT   FL G  W+P    PY+
Sbjct: 553 ALRFTPARFLRGNLWIPPNRVPYM 567

BLAST of Cmc09g0251271 vs. ExPASy TrEMBL
Match: A0A5A7U7W4 (Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold180G00090 PE=3 SV=1)

HSP 1 Score: 1127.5 bits (2915), Expect = 0.0e+00
Identity = 565/565 (100.00%), Postives = 565/565 (100.00%), Query Frame = 0

Query: 1   MVAKVVMAVTSLLVVGVVLAVFAMVNKNHSSDETADMSPKMKAVSSICSNTDYQQECQTT 60
           MVAKVVMAVTSLLVVGVVLAVFAMVNKNHSSDETADMSPKMKAVSSICSNTDYQQECQTT
Sbjct: 1   MVAKVVMAVTSLLVVGVVLAVFAMVNKNHSSDETADMSPKMKAVSSICSNTDYQQECQTT 60

Query: 61  LGNVGHNSSVDDPKEFLKAAITATIEEMKKGYNLTDNLMVEAANNASIKMTVDDCRDLLQ 120
           LGNVGHNSSVDDPKEFLKAAITATIEEMKKGYNLTDNLMVEAANNASIKMTVDDCRDLLQ
Sbjct: 61  LGNVGHNSSVDDPKEFLKAAITATIEEMKKGYNLTDNLMVEAANNASIKMTVDDCRDLLQ 120

Query: 121 SAIDELHASYSTVGDPDLHTNEDRVADIKNWLTAVISYQQSCLDGLEEFDPQLKQKMQGG 180
           SAIDELHASYSTVGDPDLHTNEDRVADIKNWLTAVISYQQSCLDGLEEFDPQLKQKMQGG
Sbjct: 121 SAIDELHASYSTVGDPDLHTNEDRVADIKNWLTAVISYQQSCLDGLEEFDPQLKQKMQGG 180

Query: 181 LDVAAKLTSNALAIVDAVSDILKSLGLQLKVQPSGRRLLGTTEVDNDGYPTWLTGADRKL 240
           LDVAAKLTSNALAIVDAVSDILKSLGLQLKVQPSGRRLLGTTEVDNDGYPTWLTGADRKL
Sbjct: 181 LDVAAKLTSNALAIVDAVSDILKSLGLQLKVQPSGRRLLGTTEVDNDGYPTWLTGADRKL 240

Query: 241 LAAKRGGVRVKPNAVVAKDGSGQYKTIAAALAAYPKALKGRYVIYVKAGIYNEYIIVTKD 300
           LAAKRGGVRVKPNAVVAKDGSGQYKTIAAALAAYPKALKGRYVIYVKAGIYNEYIIVTKD
Sbjct: 241 LAAKRGGVRVKPNAVVAKDGSGQYKTIAAALAAYPKALKGRYVIYVKAGIYNEYIIVTKD 300

Query: 301 MKNVFMYGDGPRKTIVTGRKSNRDGINTQNTATFAAIGEGFLCKSMGFTNTAGPEGHQAV 360
           MKNVFMYGDGPRKTIVTGRKSNRDGINTQNTATFAAIGEGFLCKSMGFTNTAGPEGHQAV
Sbjct: 301 MKNVFMYGDGPRKTIVTGRKSNRDGINTQNTATFAAIGEGFLCKSMGFTNTAGPEGHQAV 360

Query: 361 ALRVQSDRSAFFNCRMDGYQDTLYVQTHRQFYRNCVVSGTVDFIFGDSNTIIQNSLIIVR 420
           ALRVQSDRSAFFNCRMDGYQDTLYVQTHRQFYRNCVVSGTVDFIFGDSNTIIQNSLIIVR
Sbjct: 361 ALRVQSDRSAFFNCRMDGYQDTLYVQTHRQFYRNCVVSGTVDFIFGDSNTIIQNSLIIVR 420

Query: 421 RPMDNQQNTVTANGRADKKEISGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKEYARTVI 480
           RPMDNQQNTVTANGRADKKEISGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKEYARTVI
Sbjct: 421 RPMDNQQNTVTANGRADKKEISGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKEYARTVI 480

Query: 481 METTLGDFIQPAGYMPWSGDFALATCSYFEYGNRGPGANTKRRVRWKGVRVIGRNEAMQF 540
           METTLGDFIQPAGYMPWSGDFALATCSYFEYGNRGPGANTKRRVRWKGVRVIGRNEAMQF
Sbjct: 481 METTLGDFIQPAGYMPWSGDFALATCSYFEYGNRGPGANTKRRVRWKGVRVIGRNEAMQF 540

Query: 541 TAGPFLLGRAWLPATGGPYLLGLKN 566
           TAGPFLLGRAWLPATGGPYLLGLKN
Sbjct: 541 TAGPFLLGRAWLPATGGPYLLGLKN 565

BLAST of Cmc09g0251271 vs. ExPASy TrEMBL
Match: A0A1S3CR40 (Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103503798 PE=3 SV=1)

HSP 1 Score: 1118.6 bits (2892), Expect = 0.0e+00
Identity = 558/559 (99.82%), Postives = 559/559 (100.00%), Query Frame = 0

Query: 7   MAVTSLLVVGVVLAVFAMVNKNHSSDETADMSPKMKAVSSICSNTDYQQECQTTLGNVGH 66
           MAVTSLLVVGVVLAVFAMVNKNHSSDETADMSPKMKAVSSICSNTDYQQECQTTLGNVGH
Sbjct: 1   MAVTSLLVVGVVLAVFAMVNKNHSSDETADMSPKMKAVSSICSNTDYQQECQTTLGNVGH 60

Query: 67  NSSVDDPKEFLKAAITATIEEMKKGYNLTDNLMVEAANNASIKMTVDDCRDLLQSAIDEL 126
           NSSVDDPKEFLKAAITATIEEMKKGYNLTDNLMVEAANNASIKMTVDDCRDLLQSAIDEL
Sbjct: 61  NSSVDDPKEFLKAAITATIEEMKKGYNLTDNLMVEAANNASIKMTVDDCRDLLQSAIDEL 120

Query: 127 HASYSTVGDPDLHTNEDRVADIKNWLTAVISYQQSCLDGLEEFDPQLKQKMQGGLDVAAK 186
           HASYSTVGDPDLHTNEDRVADIKNWLTAVISYQQSCLDGLEEFDPQLKQKMQGGLDVAAK
Sbjct: 121 HASYSTVGDPDLHTNEDRVADIKNWLTAVISYQQSCLDGLEEFDPQLKQKMQGGLDVAAK 180

Query: 187 LTSNALAIVDAVSDILKSLGLQLKVQPSGRRLLGTTEVDNDGYPTWLTGADRKLLAAKRG 246
           LTSNALAIVDAVSDILKSLGLQLKVQPSGRRLLGTTEVDNDG+PTWLTGADRKLLAAKRG
Sbjct: 181 LTSNALAIVDAVSDILKSLGLQLKVQPSGRRLLGTTEVDNDGFPTWLTGADRKLLAAKRG 240

Query: 247 GVRVKPNAVVAKDGSGQYKTIAAALAAYPKALKGRYVIYVKAGIYNEYIIVTKDMKNVFM 306
           GVRVKPNAVVAKDGSGQYKTIAAALAAYPKALKGRYVIYVKAGIYNEYIIVTKDMKNVFM
Sbjct: 241 GVRVKPNAVVAKDGSGQYKTIAAALAAYPKALKGRYVIYVKAGIYNEYIIVTKDMKNVFM 300

Query: 307 YGDGPRKTIVTGRKSNRDGINTQNTATFAAIGEGFLCKSMGFTNTAGPEGHQAVALRVQS 366
           YGDGPRKTIVTGRKSNRDGINTQNTATFAAIGEGFLCKSMGFTNTAGPEGHQAVALRVQS
Sbjct: 301 YGDGPRKTIVTGRKSNRDGINTQNTATFAAIGEGFLCKSMGFTNTAGPEGHQAVALRVQS 360

Query: 367 DRSAFFNCRMDGYQDTLYVQTHRQFYRNCVVSGTVDFIFGDSNTIIQNSLIIVRRPMDNQ 426
           DRSAFFNCRMDGYQDTLYVQTHRQFYRNCVVSGTVDFIFGDSNTIIQNSLIIVRRPMDNQ
Sbjct: 361 DRSAFFNCRMDGYQDTLYVQTHRQFYRNCVVSGTVDFIFGDSNTIIQNSLIIVRRPMDNQ 420

Query: 427 QNTVTANGRADKKEISGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKEYARTVIMETTLG 486
           QNTVTANGRADKKEISGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKEYARTVIMETTLG
Sbjct: 421 QNTVTANGRADKKEISGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKEYARTVIMETTLG 480

Query: 487 DFIQPAGYMPWSGDFALATCSYFEYGNRGPGANTKRRVRWKGVRVIGRNEAMQFTAGPFL 546
           DFIQPAGYMPWSGDFALATCSYFEYGNRGPGANTKRRVRWKGVRVIGRNEAMQFTAGPFL
Sbjct: 481 DFIQPAGYMPWSGDFALATCSYFEYGNRGPGANTKRRVRWKGVRVIGRNEAMQFTAGPFL 540

Query: 547 LGRAWLPATGGPYLLGLKN 566
           LGRAWLPATGGPYLLGLKN
Sbjct: 541 LGRAWLPATGGPYLLGLKN 559

BLAST of Cmc09g0251271 vs. ExPASy TrEMBL
Match: A0A0A0KLN2 (Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_5G119700 PE=3 SV=1)

HSP 1 Score: 1047.7 bits (2708), Expect = 1.7e-302
Identity = 519/566 (91.70%), Postives = 547/566 (96.64%), Query Frame = 0

Query: 1   MVAKVVMA-VTSLLVVGVVLAVFAMVNKNHSSDETADMSPKMKAVSSICSNTDYQQECQT 60
           MV KVVM+ ++ +LVVGV LAV A+VNK+ SSDETA++SPKMKAV+SICSN DYQ ECQT
Sbjct: 1   MVGKVVMSGISLILVVGVALAVVAIVNKSDSSDETANLSPKMKAVASICSNADYQPECQT 60

Query: 61  TLGNVGHNSSVDDPKEFLKAAITATIEEMKKGYNLTDNLMVEAANNASIKMTVDDCRDLL 120
           TLG+VG+NSSV+DPKEF+KAAIT+TIEEMKKGYNLTDNLMVEAANNA+IKM+VDDC+DLL
Sbjct: 61  TLGSVGNNSSVEDPKEFIKAAITSTIEEMKKGYNLTDNLMVEAANNATIKMSVDDCKDLL 120

Query: 121 QSAIDELHASYSTVGDPDLHTNEDRVADIKNWLTAVISYQQSCLDGLEEFDPQLKQKMQG 180
           QSAIDELHASYSTVGDPDLHTNEDR+ADIKNWLTAVISYQQSCLDGLEEFDPQLKQKMQ 
Sbjct: 121 QSAIDELHASYSTVGDPDLHTNEDRIADIKNWLTAVISYQQSCLDGLEEFDPQLKQKMQD 180

Query: 181 GLDVAAKLTSNALAIVDAVSDILKSLGLQLKVQPSGRRLLGTTEVDNDGYPTWLTGADRK 240
           GLDVA KLTSNALAIV AVSDILK LGLQLKVQPSGRRLLGTTEVD+D +PTWLTG+DRK
Sbjct: 181 GLDVAGKLTSNALAIVGAVSDILKELGLQLKVQPSGRRLLGTTEVDSDSFPTWLTGSDRK 240

Query: 241 LLAAKRGGVRVKPNAVVAKDGSGQYKTIAAALAAYPKALKGRYVIYVKAGIYNEYIIVTK 300
           LLAAKRGGVRVKPN VVAKDGSGQYKTI AALAAYPKALKGRYVIYVKAG+YNE IIVTK
Sbjct: 241 LLAAKRGGVRVKPNVVVAKDGSGQYKTIGAALAAYPKALKGRYVIYVKAGVYNEPIIVTK 300

Query: 301 DMKNVFMYGDGPRKTIVTGRKSNRDGINTQNTATFAAIGEGFLCKSMGFTNTAGPEGHQA 360
           DMKN+FMYGDGPRKTIVTGRKSNRDGI TQNTA+FAAIGEGFLCKSMGFTNTAGPEGHQA
Sbjct: 301 DMKNIFMYGDGPRKTIVTGRKSNRDGITTQNTASFAAIGEGFLCKSMGFTNTAGPEGHQA 360

Query: 361 VALRVQSDRSAFFNCRMDGYQDTLYVQTHRQFYRNCVVSGTVDFIFGDSNTIIQNSLIIV 420
           VALRVQSDRSAFFNCRMDGYQDTLYVQTHRQFYRNCV+SGTVDFIFGDSNTIIQNSLIIV
Sbjct: 361 VALRVQSDRSAFFNCRMDGYQDTLYVQTHRQFYRNCVISGTVDFIFGDSNTIIQNSLIIV 420

Query: 421 RRPMDNQQNTVTANGRADKKEISGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKEYARTV 480
           RRPMDNQQNTVTANGRADKKEISGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKEYARTV
Sbjct: 421 RRPMDNQQNTVTANGRADKKEISGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKEYARTV 480

Query: 481 IMETTLGDFIQPAGYMPWSGDFALATCSYFEYGNRGPGANTKRRVRWKGVRVIGRNEAMQ 540
           IME+TLGDFIQPAGYMPWSG+FALATCSYFEYGNRGPGANT RRVRWKGVRVIGRNEAMQ
Sbjct: 481 IMESTLGDFIQPAGYMPWSGNFALATCSYFEYGNRGPGANTNRRVRWKGVRVIGRNEAMQ 540

Query: 541 FTAGPFLLGRAWLPATGGPYLLGLKN 566
           FTAGPFLLG+AWLP TGGPYLLGLKN
Sbjct: 541 FTAGPFLLGKAWLPGTGGPYLLGLKN 566

BLAST of Cmc09g0251271 vs. ExPASy TrEMBL
Match: A0A1S3CR27 (Pectinesterase OS=Cucumis melo OX=3656 GN=LOC107990290 PE=3 SV=1)

HSP 1 Score: 958.4 bits (2476), Expect = 1.4e-275
Identity = 474/567 (83.60%), Postives = 513/567 (90.48%), Query Frame = 0

Query: 1   MVAKVVM-AVTSLLVVGVVLAVFAMVNKNHSSD-ETADMSPKMKAVSSICSNTDYQQECQ 60
           MV KV++  +T +LVVGV LAV A+V+ N SSD  T D+SPKMKAVS+IC+N +YQQECQ
Sbjct: 1   MVNKVILPGITLILVVGVALAVVAIVHSNKSSDAATEDLSPKMKAVSTICANANYQQECQ 60

Query: 61  TTLGNVGHNSSVDDPKEFLKAAITATIEEMKKGYNLTDNLMVEAANNASIKMTVDDCRDL 120
            TL N  HN+S DDPKE+++AAI +TIEE+KKGYNLTD  M+EAANN SIKM V+DCRDL
Sbjct: 61  NTLSNAAHNASSDDPKEYVRAAILSTIEEVKKGYNLTDGFMIEAANNRSIKMGVEDCRDL 120

Query: 121 LQSAIDELHASYSTVGDPDLHTNEDRVADIKNWLTAVISYQQSCLDGLEEFDPQLKQKMQ 180
           LQ AID+L ASYSTVG+PDLHTN DRVADIKNWLT+VISYQQSCLDGLEEFDPQLKQKMQ
Sbjct: 121 LQFAIDQLQASYSTVGEPDLHTNADRVADIKNWLTSVISYQQSCLDGLEEFDPQLKQKMQ 180

Query: 181 GGLDVAAKLTSNALAIVDAVSDILKSLGLQLKVQPSGRRLLGTTEVDNDGYPTWLTGADR 240
            GL  A KLTSNALAIVDAVSDIL S GLQLK QPSGRRLLGTTEV  DGYPTWLTGADR
Sbjct: 181 DGLSDAGKLTSNALAIVDAVSDILASFGLQLKAQPSGRRLLGTTEVGKDGYPTWLTGADR 240

Query: 241 KLLAAKRGGVRVKPNAVVAKDGSGQYKTIAAALAAYPKALKGRYVIYVKAGIYNEYIIVT 300
           KLLA++ GG +VKPNAVVAKDGSGQ+KTI+AALAAYPKAL+GRYVIYVKAGIYNEY+ +T
Sbjct: 241 KLLASRGGGAKVKPNAVVAKDGSGQFKTISAALAAYPKALRGRYVIYVKAGIYNEYVHIT 300

Query: 301 KDMKNVFMYGDGPRKTIVTGRKSNRDGINTQNTATFAAIGEGFLCKSMGFTNTAGPEGHQ 360
           KDMKN+FMYGDGP+KTIVTG KSNR G  TQ+TATF AIGEGFLCKSMGF NTAGPEGHQ
Sbjct: 301 KDMKNIFMYGDGPKKTIVTGNKSNRGGFTTQDTATFIAIGEGFLCKSMGFQNTAGPEGHQ 360

Query: 361 AVALRVQSDRSAFFNCRMDGYQDTLYVQTHRQFYRNCVVSGTVDFIFGDSNTIIQNSLII 420
           AVALRVQSDRSAFFNCRMDGYQDTLYVQT RQFYRNCV+SGTVDFIFGDS TIIQNSLII
Sbjct: 361 AVALRVQSDRSAFFNCRMDGYQDTLYVQTQRQFYRNCVISGTVDFIFGDSTTIIQNSLII 420

Query: 421 VRRPMDNQQNTVTANGRADKKEISGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKEYART 480
           VRRPMDNQQNTVTANGRAD KE+SGLVIHNCRIVPEQKLFAERFKIPTFLGRPWK+YART
Sbjct: 421 VRRPMDNQQNTVTANGRADPKEVSGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKQYART 480

Query: 481 VIMETTLGDFIQPAGYMPWSGDFALATCSYFEYGNRGPGANTKRRVRWKGVRVIGRNEAM 540
           VIME+TLGDFIQP GYMPWSG+FAL TC Y EYGNRGPGA T RRVRWKGV+VIGRNEAM
Sbjct: 481 VIMESTLGDFIQPVGYMPWSGNFALETCLYLEYGNRGPGAVTNRRVRWKGVKVIGRNEAM 540

Query: 541 QFTAGPFLLGRAWLPATGGPYLLGLKN 566
           QFTAGPFL GRAWLP TGGPYLLGLK+
Sbjct: 541 QFTAGPFLQGRAWLPTTGGPYLLGLKS 567

BLAST of Cmc09g0251271 vs. ExPASy TrEMBL
Match: A0A5A7U9J8 (Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold180G00100 PE=3 SV=1)

HSP 1 Score: 956.8 bits (2472), Expect = 4.0e-275
Identity = 473/567 (83.42%), Postives = 513/567 (90.48%), Query Frame = 0

Query: 1   MVAKVVM-AVTSLLVVGVVLAVFAMVNKNHSSD-ETADMSPKMKAVSSICSNTDYQQECQ 60
           MV KV++  ++ +LVVGV LAV A+V+ N SSD  T D+SPKMKAVS+IC+N +YQQECQ
Sbjct: 1   MVNKVILPGISLILVVGVALAVVAIVHSNKSSDAATEDLSPKMKAVSTICANANYQQECQ 60

Query: 61  TTLGNVGHNSSVDDPKEFLKAAITATIEEMKKGYNLTDNLMVEAANNASIKMTVDDCRDL 120
            TL N  HN+S DDPKE+++AAI +TIEE+KKGYNLTD  M+EAANN SIKM V+DCRDL
Sbjct: 61  NTLSNAAHNASSDDPKEYVRAAILSTIEEVKKGYNLTDGFMIEAANNRSIKMGVEDCRDL 120

Query: 121 LQSAIDELHASYSTVGDPDLHTNEDRVADIKNWLTAVISYQQSCLDGLEEFDPQLKQKMQ 180
           LQ AID+L ASYSTVG+PDLHTN DRVADIKNWLT+VISYQQSCLDGLEEFDPQLKQKMQ
Sbjct: 121 LQFAIDQLQASYSTVGEPDLHTNADRVADIKNWLTSVISYQQSCLDGLEEFDPQLKQKMQ 180

Query: 181 GGLDVAAKLTSNALAIVDAVSDILKSLGLQLKVQPSGRRLLGTTEVDNDGYPTWLTGADR 240
            GL  A KLTSNALAIVDAVSDIL S GLQLK QPSGRRLLGTTEV  DGYPTWLTGADR
Sbjct: 181 DGLSDAGKLTSNALAIVDAVSDILASFGLQLKAQPSGRRLLGTTEVGKDGYPTWLTGADR 240

Query: 241 KLLAAKRGGVRVKPNAVVAKDGSGQYKTIAAALAAYPKALKGRYVIYVKAGIYNEYIIVT 300
           KLLA++ GG +VKPNAVVAKDGSGQ+KTI+AALAAYPKAL+GRYVIYVKAGIYNEY+ +T
Sbjct: 241 KLLASRGGGAKVKPNAVVAKDGSGQFKTISAALAAYPKALRGRYVIYVKAGIYNEYVHIT 300

Query: 301 KDMKNVFMYGDGPRKTIVTGRKSNRDGINTQNTATFAAIGEGFLCKSMGFTNTAGPEGHQ 360
           KDMKN+FMYGDGP+KTIVTG KSNR G  TQ+TATF AIGEGFLCKSMGF NTAGPEGHQ
Sbjct: 301 KDMKNIFMYGDGPKKTIVTGNKSNRGGFTTQDTATFIAIGEGFLCKSMGFQNTAGPEGHQ 360

Query: 361 AVALRVQSDRSAFFNCRMDGYQDTLYVQTHRQFYRNCVVSGTVDFIFGDSNTIIQNSLII 420
           AVALRVQSDRSAFFNCRMDGYQDTLYVQT RQFYRNCV+SGTVDFIFGDS TIIQNSLII
Sbjct: 361 AVALRVQSDRSAFFNCRMDGYQDTLYVQTQRQFYRNCVISGTVDFIFGDSTTIIQNSLII 420

Query: 421 VRRPMDNQQNTVTANGRADKKEISGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKEYART 480
           VRRPMDNQQNTVTANGRAD KE+SGLVIHNCRIVPEQKLFAERFKIPTFLGRPWK+YART
Sbjct: 421 VRRPMDNQQNTVTANGRADPKEVSGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKQYART 480

Query: 481 VIMETTLGDFIQPAGYMPWSGDFALATCSYFEYGNRGPGANTKRRVRWKGVRVIGRNEAM 540
           VIME+TLGDFIQP GYMPWSG+FAL TC Y EYGNRGPGA T RRVRWKGV+VIGRNEAM
Sbjct: 481 VIMESTLGDFIQPVGYMPWSGNFALETCLYLEYGNRGPGAVTNRRVRWKGVKVIGRNEAM 540

Query: 541 QFTAGPFLLGRAWLPATGGPYLLGLKN 566
           QFTAGPFL GRAWLP TGGPYLLGLK+
Sbjct: 541 QFTAGPFLQGRAWLPTTGGPYLLGLKS 567

BLAST of Cmc09g0251271 vs. TAIR 10
Match: AT3G05610.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 454.9 bits (1169), Expect = 9.4e-128
Identity = 229/562 (40.75%), Postives = 347/562 (61.74%), Query Frame = 0

Query: 5   VVMAVTSLLVVGVVLAVFAMVNKN-HSSDE--TADMSPKMKAVSSICSNTDYQQECQTTL 64
           +V+ ++S+L++ +V+AV   V+ N H  D    A+++  +KAV  +C+ TDY++ C+ TL
Sbjct: 16  IVITISSVLLISMVVAVTVGVSLNKHDGDSKGKAEVNASVKAVKDVCAPTDYRKTCEDTL 75

Query: 65  GNVGHNSSVDDPKEFLKAAITATIEEMKKGYNLTDNLMVEAANNASIKMTVDDCRDLLQS 124
              G N++  DP E +K A   T++++      +  +M E   ++  +M +D C++L+  
Sbjct: 76  IKNGKNTT--DPMELVKTAFNVTMKQITDAAKKSQTIM-ELQKDSRTRMALDQCKELMDY 135

Query: 125 AIDELHASYSTVGDPDLHTNEDRVADIKNWLTAVISYQQSCLDGLEEFDPQLKQKMQGGL 184
           A+DEL  S+  +G  + H  ++ + +++ WL+A IS++++CL+G +       + M+  L
Sbjct: 136 ALDELSNSFEELGKFEFHLLDEALINLRIWLSAAISHEETCLEGFQGTQGNAGETMKKAL 195

Query: 185 DVAAKLTSNALAIVDAVSDILKSLGLQLKVQPSGRRLLGTTEVDNDGYPTWLTGADRKLL 244
             A +LT N LAI+  +S+ +  + +      + RRLL       +G+P+W+    RKLL
Sbjct: 196 KTAIELTHNGLAIISEMSNFVGQMQIP---GLNSRRLLA------EGFPSWVDQRGRKLL 255

Query: 245 AAKRGGVRVKPNAVVAKDGSGQYKTIAAALAAYPKALKGRYVIYVKAGIYNEYIIVTKDM 304
            A      VKP+ VVA+DGSGQYKTI  AL   PK     +V+++KAG+Y EY+ V K M
Sbjct: 256 QAAAAYSDVKPDIVVAQDGSGQYKTINEALQFVPKKRNTTFVVHIKAGLYKEYVQVNKTM 315

Query: 305 KNVFMYGDGPRKTIVTGRKSNRDGINTQNTATFAAIGEGFLCKSMGFTNTAGPEGHQAVA 364
            ++   GDGP KTI++G K+ +DGI T  TAT A +G  F+ K++GF NTAG   HQAVA
Sbjct: 316 SHLVFIGDGPDKTIISGNKNYKDGITTYRTATVAIVGNYFIAKNIGFENTAGAIKHQAVA 375

Query: 365 LRVQSDRSAFFNCRMDGYQDTLYVQTHRQFYRNCVVSGTVDFIFGDSNTIIQNSLIIVRR 424
           +RVQSD S FFNCR DGYQDTLY  +HRQF+R+C +SGT+DF+FGD+  + QN  ++VR+
Sbjct: 376 VRVQSDESIFFNCRFDGYQDTLYTHSHRQFFRDCTISGTIDFLFGDAAAVFQNCTLLVRK 435

Query: 425 PMDNQQNTVTANGRADKKEISGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKEYARTVIM 484
           P+ NQ   +TA+GR D +E +G V   C I  E    A +     +LGRPWKEY+RT+IM
Sbjct: 436 PLPNQACPITAHGRKDPRESTGFVFQGCTIAGEPDYLAVKETSKAYLGRPWKEYSRTIIM 495

Query: 485 ETTLGDFIQPAGYMPWSGDFALATCSYFEYGNRGPGANTKRRVRWKGVRVIGRNEAMQFT 544
            T + DF+QP G+ PW GDF L T  Y E  N GPG+    RV W G++ +   + ++FT
Sbjct: 496 NTFIPDFVQPQGWQPWLGDFGLKTLFYSEVQNTGPGSALANRVTWAGIKTLSEEDILKFT 555

Query: 545 AGPFLLGRAWLPATGGPYLLGL 564
              ++ G  W+P  G PY  GL
Sbjct: 556 PAQYIQGDDWIPGKGVPYTTGL 565

BLAST of Cmc09g0251271 vs. TAIR 10
Match: AT4G33230.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 452.6 bits (1163), Expect = 4.7e-127
Identity = 242/536 (45.15%), Postives = 338/536 (63.06%), Query Frame = 0

Query: 30  SSDETADMSPKMKAVSSICSNTDYQQECQTTLGN-VGHNSSVDDPKEFLKAAITATIEEM 89
           S+ +T       K + ++C++T Y+  CQ TL N    ++   DP+  LK+AI A  +++
Sbjct: 81  SAAQTVKAGQVDKIIQTLCNSTLYKPTCQNTLKNETKKDTPQTDPRSLLKSAIVAVNDDL 140

Query: 90  KKGYNLTDNLMVEAANNASIKMTVDDCRDLLQSAIDELHASYSTVGDPDLHTNEDRVADI 149
            + +    +L  E   N   K  +  C+ L+  A +EL  S   + D +++     V D+
Sbjct: 141 DQVFKRVLSLKTE---NKDDKDAIAQCKLLVDEAKEELGTSMKRINDSEVNNFAKIVPDL 200

Query: 150 KNWLTAVISYQQSCLDGLEEFDPQLKQKMQGGLDVAAKLTSNALAIVDAVSDILKSLGLQ 209
            +WL+AV+SYQ++C+DG EE   +LK +++   + +  LTSN+LA++ ++   L S+   
Sbjct: 201 DSWLSAVMSYQETCVDGFEE--GKLKTEIRKNFNSSQVLTSNSLAMIKSLDGYLSSVP-- 260

Query: 210 LKVQPSGRRLLGTTEVDNDGYPTWLTGADRKLLAAKRGGVRVKPNAVVAKDGSGQYKTIA 269
            KV+        ++  + D   +WL+  +R++L A      +KPNA VAKDGSG + TI 
Sbjct: 261 -KVKTRLLLEARSSAKETDHITSWLSNKERRMLKAV-DVKALKPNATVAKDGSGNFTTIN 320

Query: 270 AALAAYPKALKGRYVIYVKAGIYNEYIIVTKDMKNVFMYGDGPRKTIVTGRKSNRDGINT 329
           AAL A P   +GRY IY+K GIY+E +I+ K   NV M GDG +KTIVTG KS+   I T
Sbjct: 321 AALKAMPAKYQGRYTIYIKHGIYDESVIIDKKKPNVTMVGDGSQKTIVTGNKSHAKKIRT 380

Query: 330 QNTATFAAIGEGFLCKSMGFTNTAGPEGHQAVALRVQSDRSAFFNCRMDGYQDTLYVQTH 389
             TATF A GEGF+ +SMGF NTAGPEGHQAVA+RVQSDRS F NCR +GYQDTLY  TH
Sbjct: 381 FLTATFVAQGEGFMAQSMGFRNTAGPEGHQAVAIRVQSDRSVFLNCRFEGYQDTLYAYTH 440

Query: 390 RQFYRNCVVSGTVDFIFGDSNTIIQNSLIIVRRPMDNQQNTVTANGRADKKEISGLVIHN 449
           RQ+YR+CV+ GTVDFIFGD+  I QN  I +R+ +  Q+NTVTA GR DK + +G VIHN
Sbjct: 441 RQYYRSCVIIGTVDFIFGDAAAIFQNCDIFIRKGLPGQKNTVTAQGRVDKFQTTGFVIHN 500

Query: 450 CRIVPEQKLFAERFKIPTFLGRPWKEYARTVIMETTLGDFIQPAGYMPW-SGDFALATCS 509
           C + P + L   + +  ++LGRPWK ++RTV+ME+T+ D I P G++ W   DFA+ T S
Sbjct: 501 CTVAPNEDLKPVKAQFKSYLGRPWKPHSRTVVMESTIEDVIDPVGWLRWQETDFAIDTLS 560

Query: 510 YFEYGNRGPGANTKRRVRWKGVRVIGRNEAMQFTAGPFLLGRAWLPATGGPYLLGL 564
           Y EY N GP   T  RV+W G RV+ + EAM+FT GPFL G  W+ A G P  LGL
Sbjct: 561 YAEYKNDGPSGATAARVKWPGFRVLNKEEAMKFTVGPFLQGE-WIQAIGSPVKLGL 606

BLAST of Cmc09g0251271 vs. TAIR 10
Match: AT2G26450.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 448.0 bits (1151), Expect = 1.1e-125
Identity = 238/524 (45.42%), Postives = 339/524 (64.69%), Query Frame = 0

Query: 42  KAVSSICSNTDYQQECQTTLGN-VGHNSSVDDPKEFLKAAITATIEEMKKGYNLTDNLMV 101
           K + ++CS+T Y Q C+ TL N      ++D+P  FLK+AI A  E++     + + ++ 
Sbjct: 107 KIIQTLCSSTLYMQICEKTLKNRTDKGFALDNPTTFLKSAIEAVNEDLDL---VLEKVLS 166

Query: 102 EAANNASIKMTVDDCRDLLQSAIDELHASYSTVGDPDLHTNEDRVADIKNWLTAVISYQQ 161
               N   K  ++ C+ L++ A +E  AS + +   ++++ E  V D+++WL+AV+SYQ+
Sbjct: 167 LKTENQDDKDAIEQCKLLVEDAKEETVASLNKINVTEVNSFEKVVPDLESWLSAVMSYQE 226

Query: 162 SCLDGLEEFDPQLKQKMQGGLDVAAKLTSNALAIVDAVSDILKSLGLQLKVQPSGRRLLG 221
           +CLDG EE    LK +++  ++ +  LTSN+LA++   ++ L  +   +KV    R LL 
Sbjct: 227 TCLDGFEE--GNLKSEVKTSVNSSQVLTSNSLALIKTFTENLSPV---MKVVE--RHLL- 286

Query: 222 TTEVDNDGYPTWLTGADRKLLAAKRGGVRVKPNAVVAKDGSGQYKTIAAALAAYPKALKG 281
                 D  P+W++  DR++L A      +KPNA VAKDGSG + TI  AL A P+  +G
Sbjct: 287 ------DDIPSWVSNDDRRMLRAV-DVKALKPNATVAKDGSGDFTTINDALRAMPEKYEG 346

Query: 282 RYVIYVKAGIYNEYIIVTKDMKNVFMYGDGPRKTIVTGRKSNRDGINTQNTATFAAIGEG 341
           RY+IYVK GIY+EY+ V K   N+ M GDG +KTIVTG KS+   I T  TATF A GEG
Sbjct: 347 RYIIYVKQGIYDEYVTVDKKKANLTMVGDGSQKTIVTGNKSHAKKIRTFLTATFVAQGEG 406

Query: 342 FLCKSMGFTNTAGPEGHQAVALRVQSDRSAFFNCRMDGYQDTLYVQTHRQFYRNCVVSGT 401
           F+ +SMGF NTAGPEGHQAVA+RVQSDRS F NCR +GYQDTLY  THRQ+YR+CV+ GT
Sbjct: 407 FMAQSMGFRNTAGPEGHQAVAIRVQSDRSIFLNCRFEGYQDTLYAYTHRQYYRSCVIVGT 466

Query: 402 VDFIFGDSNTIIQNSLIIVRRPMDNQQNTVTANGRADKKEISGLVIHNCRIVPEQKLFAE 461
           +DFIFGD+  I QN  I +R+ +  Q+NTVTA GR DK + +G V+HNC+I   + L   
Sbjct: 467 IDFIFGDAAAIFQNCNIFIRKGLPGQKNTVTAQGRVDKFQTTGFVVHNCKIAANEDLKPV 526

Query: 462 RFKIPTFLGRPWKEYARTVIMETTLGDFIQPAGYMPW-SGDFALATCSYFEYGNRGPGAN 521
           + +  ++LGRPWK Y+RT+IME+ + + I P G++ W   DFA+ T  Y EY N+G   +
Sbjct: 527 KEEYKSYLGRPWKNYSRTIIMESKIENVIDPVGWLRWQETDFAIDTLYYAEYNNKGSSGD 586

Query: 522 TKRRVRWKGVRVIGRNEAMQFTAGPFLLGRAWLPATGGPYLLGL 564
           T  RV+W G +VI + EA+ +T GPFL G  W+ A+G P  LGL
Sbjct: 587 TTSRVKWPGFKVINKEEALNYTVGPFLQG-DWISASGSPVKLGL 611

BLAST of Cmc09g0251271 vs. TAIR 10
Match: AT5G27870.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 446.4 bits (1147), Expect = 3.3e-125
Identity = 226/560 (40.36%), Postives = 349/560 (62.32%), Query Frame = 0

Query: 5   VVMAVTSLLVVGVVLAVFAMVNKNHSSDE-TADMSPKMKAVSSICSNTDYQQECQTTLGN 64
           V+++++S+L++ +V+AV   V+ N S +    +++  +KA+  +C+ TDY++ C+ TL  
Sbjct: 17  VIISISSVLLISMVVAVTIGVSVNKSDNAGDEEITTSVKAIKDVCAPTDYKETCEDTLRK 76

Query: 65  VGHNSSVDDPKEFLKAAITATIEEMKKGYNLTDNLMVEAANNASIKMTVDDCRDLLQSAI 124
              ++S  DP E +K A  AT++++      +   M+E   +   KM +D C++L+  AI
Sbjct: 77  DAKDTS--DPLELVKTAFNATMKQISDVAKKSQT-MIELQKDPRAKMALDQCKELMDYAI 136

Query: 125 DELHASYSTVGDPDLHTNEDRVADIKNWLTAVISYQQSCLDGLEEFDPQLKQKMQGGLDV 184
            EL  S+  +G  + H  ++ +  ++ WL+A IS++Q+CLDG +       + ++  L  
Sbjct: 137 GELSKSFEELGKFEFHKVDEALVKLRIWLSATISHEQTCLDGFQGTQGNAGETIKKALKT 196

Query: 185 AAKLTSNALAIVDAVSDILKSLGLQLKVQPSGRRLLGTTEVDNDGYPTWLTGADRKLLAA 244
           A +LT N LA+V  +S+ L  + +    + + RRLL      +  +P+W+    R+LL A
Sbjct: 197 AVQLTHNGLAMVTEMSNYLGQMQIP---EMNSRRLL------SQEFPSWMDARARRLLNA 256

Query: 245 KRGGVRVKPNAVVAKDGSGQYKTIAAALAAYPKALKGRYVIYVKAGIYNEYIIVTKDMKN 304
                 VKP+ VVA+DGSGQYKTI  AL   PK     +V+++K GIY EY+ V + M +
Sbjct: 257 PMS--EVKPDIVVAQDGSGQYKTINEALNFVPKKKNTTFVVHIKEGIYKEYVQVNRSMTH 316

Query: 305 VFMYGDGPRKTIVTGRKSNRDGINTQNTATFAAIGEGFLCKSMGFTNTAGPEGHQAVALR 364
           +   GDGP KT+++G KS +DGI T  TAT A +G+ F+ K++ F NTAG   HQAVA+R
Sbjct: 317 LVFIGDGPDKTVISGSKSYKDGITTYKTATVAIVGDHFIAKNIAFENTAGAIKHQAVAIR 376

Query: 365 VQSDRSAFFNCRMDGYQDTLYVQTHRQFYRNCVVSGTVDFIFGDSNTIIQNSLIIVRRPM 424
           V +D S F+NC+ DGYQDTLY  +HRQFYR+C +SGT+DF+FGD+  + QN  ++VR+P+
Sbjct: 377 VLADESIFYNCKFDGYQDTLYAHSHRQFYRDCTISGTIDFLFGDAAAVFQNCTLLVRKPL 436

Query: 425 DNQQNTVTANGRADKKEISGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKEYARTVIMET 484
            NQ   +TA+GR D +E +G V+  C IV E    A + +  T+LGRPWKEY+RT+IM T
Sbjct: 437 LNQACPITAHGRKDPRESTGFVLQGCTIVGEPDYLAVKEQSKTYLGRPWKEYSRTIIMNT 496

Query: 485 TLGDFIQPAGYMPWSGDFALATCSYFEYGNRGPGANTKRRVRWKGVRVIGRNEAMQFTAG 544
            + DF+ P G+ PW G+F L T  Y E  N GPGA   +RV W G++ +   E ++FT  
Sbjct: 497 FIPDFVPPEGWQPWLGEFGLNTLFYSEVQNTGPGAAITKRVTWPGIKKLSDEEILKFTPA 556

Query: 545 PFLLGRAWLPATGGPYLLGL 564
            ++ G AW+P  G PY+LGL
Sbjct: 557 QYIQGDAWIPGKGVPYILGL 562

BLAST of Cmc09g0251271 vs. TAIR 10
Match: AT5G49180.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 439.1 bits (1128), Expect = 5.3e-123
Identity = 239/564 (42.38%), Postives = 339/564 (60.11%), Query Frame = 0

Query: 5   VVMAVTSLLVVGVVLAVFAMVNKNHSSDE---TADMSPKMKAVSSICSNTDYQQECQTTL 64
           ++  V + L+V +V+AV    ++N S  E      +     AV ++C+ TDY++ C  +L
Sbjct: 13  IIAGVITALLVLMVVAVGITTSRNTSHSEKIVPVQIKTATTAVEAVCAPTDYKETCVNSL 72

Query: 65  GNVGHNSSVDDPKEFLKAAITATIEEMKKGYNLTD-NLMVEAANNASIKMTVDDCRDLLQ 124
                +S+   P + +K     TI  ++         L  +AAN+   K  ++ C  L+ 
Sbjct: 73  MKASPDST--QPLDLIKLGFNVTIRSIEDSIKKASVELTAKAANDKDTKGALELCEKLMN 132

Query: 125 SAIDELHASYSTVGDPDLHTNEDRVADIKNWLTAVISYQQSCLDGLEEFDPQLKQKMQGG 184
            A D+L           +   ED V D++ WL+  I+YQQ+C+D  EE + +L Q MQ  
Sbjct: 133 DATDDLKKCLDNFDGFSIPQIEDFVEDLRVWLSGSIAYQQTCMDTFEETNSKLSQDMQKI 192

Query: 185 LDVAAKLTSNALAIVDAVSDILKSL---GLQLKVQPSGRRLLGTTEVDNDGYPTWLTGAD 244
              + +LTSN LA++  +S++L      G+   +    R+LL       DG P+W+    
Sbjct: 193 FKTSRELTSNGLAMITNISNLLGEFNVTGVTGDLGKYARKLLSA----EDGIPSWVGPNT 252

Query: 245 RKLLAAKRGGVRVKPNAVVAKDGSGQYKTIAAALAAYPKALKGRYVIYVKAGIYNEYIIV 304
           R+L+A K G   VK N VVA DGSGQYKTI  AL A PKA +  +VIY+K G+YNE + V
Sbjct: 253 RRLMATKGG---VKANVVVAHDGSGQYKTINEALNAVPKANQKPFVIYIKQGVYNEKVDV 312

Query: 305 TKDMKNVFMYGDGPRKTIVTGRKSNRDG-INTQNTATFAAIGEGFLCKSMGFTNTAGPEG 364
           TK M +V   GDGP KT +TG  +   G + T  TAT A  G+ F  K++GF NTAGPEG
Sbjct: 313 TKKMTHVTFIGDGPTKTKITGSLNYYIGKVKTYLTATVAINGDNFTAKNIGFENTAGPEG 372

Query: 365 HQAVALRVQSDRSAFFNCRMDGYQDTLYVQTHRQFYRNCVVSGTVDFIFGDSNTIIQNSL 424
           HQAVALRV +D + F+NC++DGYQDTLYV +HRQF+R+C VSGTVDFIFGD   ++QN  
Sbjct: 373 HQAVALRVSADLAVFYNCQIDGYQDTLYVHSHRQFFRDCTVSGTVDFIFGDGIVVLQNCN 432

Query: 425 IIVRRPMDNQQNTVTANGRADKKEISGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKEYA 484
           I+VR+PM +Q   +TA GR+DK+E +GLV+ NC I  E      +     +LGRPWKE++
Sbjct: 433 IVVRKPMKSQSCMITAQGRSDKRESTGLVLQNCHITGEPAYIPVKSINKAYLGRPWKEFS 492

Query: 485 RTVIMETTLGDFIQPAGYMPWSGDFALATCSYFEYGNRGPGANTKRRVRWKGVRVIGRNE 544
           RT+IM TT+ D I PAG++PW+GDFAL T  Y EY N GPG+N  +RV+W G++ +   +
Sbjct: 493 RTIIMGTTIDDVIDPAGWLPWNGDFALNTLYYAEYENNGPGSNQAQRVKWPGIKKLSPKQ 552

Query: 545 AMQFTAGPFLLGRAWLPATGGPYL 561
           A++FT   FL G  W+P    PY+
Sbjct: 553 ALRFTPARFLRGNLWIPPNRVPYM 567

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAA0050276.10.0e+00100.00pectinesterase-like [Cucumis melo var. makuwa] >TYJ98131.1 pectinesterase-like [... [more]
XP_008466382.20.0e+0099.82PREDICTED: pectinesterase-like [Cucumis melo][more]
KGN49779.13.5e-30291.70hypothetical protein Csa_017783 [Cucumis sativus][more]
XP_038875879.18.7e-28586.75LOW QUALITY PROTEIN: pectinesterase-like [Benincasa hispida][more]
XP_008466374.12.8e-27583.60PREDICTED: pectinesterase-like [Cucumis melo][more]
Match NameE-valueIdentityDescription
Q8GX861.3e-12640.75Probable pectinesterase/pectinesterase inhibitor 21 OS=Arabidopsis thaliana OX=3... [more]
Q9SMY66.6e-12645.15Putative pectinesterase/pectinesterase inhibitor 45 OS=Arabidopsis thaliana OX=3... [more]
Q7Y2011.6e-12445.42Probable pectinesterase/pectinesterase inhibitor 13 OS=Arabidopsis thaliana OX=3... [more]
Q3E8Z84.7e-12440.36Putative pectinesterase/pectinesterase inhibitor 28 OS=Arabidopsis thaliana OX=3... [more]
Q9FJ217.5e-12242.38Probable pectinesterase/pectinesterase inhibitor 58 OS=Arabidopsis thaliana OX=3... [more]
Match NameE-valueIdentityDescription
A0A5A7U7W40.0e+00100.00Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold180G00090... [more]
A0A1S3CR400.0e+0099.82Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103503798 PE=3 SV=1[more]
A0A0A0KLN21.7e-30291.70Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_5G119700 PE=3 SV=1[more]
A0A1S3CR271.4e-27583.60Pectinesterase OS=Cucumis melo OX=3656 GN=LOC107990290 PE=3 SV=1[more]
A0A5A7U9J84.0e-27583.42Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold180G00100... [more]
Match NameE-valueIdentityDescription
AT3G05610.19.4e-12840.75Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT4G33230.14.7e-12745.15Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT2G26450.11.1e-12545.42Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT5G27870.13.3e-12540.36Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT5G49180.15.3e-12342.38Plant invertase/pectin methylesterase inhibitor superfamily [more]
InterPro
Analysis Name: InterPro Annotations of Melon (Charmono) v1.1
Date Performed: 2022-10-13
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006501Pectinesterase inhibitor domainSMARTSM00856PMEI_2coord: 39..194
e-value: 2.4E-35
score: 133.4
IPR006501Pectinesterase inhibitor domainPFAMPF04043PMEIcoord: 43..194
e-value: 8.3E-26
score: 91.1
IPR006501Pectinesterase inhibitor domainTIGRFAMTIGR01614TIGR01614coord: 15..196
e-value: 1.1E-20
score: 71.7
IPR000070Pectinesterase, catalyticPFAMPF01095Pectinesterasecoord: 253..548
e-value: 3.7E-130
score: 433.0
IPR035513Invertase/pectin methylesterase inhibitor domain superfamilyGENE3D1.20.140.40Invertase/pectin methylesterase inhibitor family proteincoord: 41..199
e-value: 5.2E-30
score: 106.4
IPR035513Invertase/pectin methylesterase inhibitor domain superfamilySUPERFAMILY101148Plant invertase/pectin methylesterase inhibitorcoord: 42..198
IPR012334Pectin lyase foldGENE3D2.160.20.10coord: 247..563
e-value: 6.1E-109
score: 366.2
NoneNo IPR availablePANTHERPTHR31707PECTINESTERASEcoord: 10..563
NoneNo IPR availablePANTHERPTHR31707:SF164PECTINESTERASE 4-RELATEDcoord: 10..563
NoneNo IPR availableCDDcd15798PMEI-like_3coord: 46..199
e-value: 5.34452E-49
score: 164.919
IPR033131Pectinesterase, Asp active sitePROSITEPS00503PECTINESTERASE_2coord: 397..406
IPR011050Pectin lyase fold/virulence factorSUPERFAMILY51126Pectin lyase-likecoord: 250..562

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cmc09g0251271.1Cmc09g0251271.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0042545 cell wall modification
biological_process GO:0043086 negative regulation of catalytic activity
biological_process GO:0045490 pectin catabolic process
cellular_component GO:0005576 extracellular region
molecular_function GO:0045330 aspartyl esterase activity
molecular_function GO:0030599 pectinesterase activity
molecular_function GO:0046910 pectinesterase inhibitor activity
molecular_function GO:0004857 enzyme inhibitor activity