Cmc09g0251221 (gene) Melon (Charmono) v1.1

Overview


Name	Cmc09g0251221
Type	gene
Organism	Cucumis melo L. var. cantalupensis cv. Charmono (Melon (Charmono) v1.1)
Description	Prolyl-tRNA synthetase
Location	CMiso1.1chr09: 17302470 .. 17304708 (-)
RNA-Seq Expression	Cmc09g0251221
Synteny	Cmc09g0251221

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

GCATATATGAAGAATACCTGGCAATTCCTGTTATCAAAGGCAAGAAGAGTGAGATGGAGAAGTTTGCTGGTGGTCTTTACACCACAAGCGTTGAGGTATAAGCTGACCGAATGCTATGACCCATCTTCAGCATCGCAAATTGATATCCATCACTTATTTAAGTTGTATAAAACCATTGTTCCGTTTGTGATATGCAGGCATTTATCCCAAACACTGGTCGTGGTATTCAAGGTGCAACTTCACATTGTTTGGGTCAAAATTTTGCAAAAATGTTTGAAATCAACTTTGAAAATGAAAAGGGGGAGAAAGCTATGGTCTGGCAAAATTCGTGGGCCTATAGTACCAGAACGGTAATATTTTGATACTTGCATTTGTCTTAGCCTTTGGTAACTTGTTAGAGTGAATTTCTTCATCTTGTTAATCTAGCCTTCCGAACTGTTTGTAGATTGGTGTGATGGTTATGGTTCACGGTGATGACAAGGGATTGGTGATGCCTCCCAAAGTTGCATCAGTGCAAGTCATTATAGTTCCTGTTCCCTACAAAGATGCAGACACTCGAGGAATTTTTGATGTTTGTTCTGCCACTTCAGATGCATTGTCCAAAGCAGGAATTCGTGCAGAGGTAGATATTAGGGAGAACTATTCTCCTGGATGGAAGTACTCCCACTGGGAAATGAAAGGTGTTCCACTCAGGATTGAAATAGGGCCTAAGGACTTGGCGAACAATCAGGTTAGATATTTTGTCTATTAATTTTCTGTTCTGTCTCTAGTGAAATACTGTTTTCTCCTTGCTAATAATTGTTAATGTCTGATTTGGATTGTGTTGATGCATAGTGCCAATATATAATTGTGTACTTTCTTTTTCTAGGTACGTGCAGTTCGCCGTGATAATTCTGCAAAGAAGGACATACCTAGGGCTTTGTTGGTTGAACAAGTGAAAGAATTGCTAGAAAGTATTCAACAAAGCCTTTTTGATGCGGCAAAAGAAAAACGAGATGCATGCATTCAGGTTGTTAATACATGGGAGGAGTTTACTGAAGCCCTCGGTCAGAAGAAAATGATATTAGCTCCATGGTGTAATGAAGAGGTACAGCTTCAGTTTTTATTGTCGCTGTTTTGGAATTCCACTTGTGTTGTTTATTACATATTAAGGACTGCCTACTTTTTGGTTCCGACTTTTCATGAGGCCACCAAAATTTTATTTGTTAGTTGAAGGGATCCTGGTTGCTATGCTTTTCCACTAGTCAAATACGCTTTCATATGCTGAAATCTATTTGATGAATCAACTATAGAGTCCTGGTTTGTAAAGCTTGTTTTTGTAGGTAGAAAGGGAGATTTTGTTGCAATCTTAGCTGCTGCAGCGGAGATAATGTTTCATTAGGATGATTTCTATAGAATTGATGGGGTAATTATCAATAAAATTTTGAACTGCTTTGATCACTTGGTTGAGTCGATGCATTTTTAAGTATTTAGTTTCTTCTGAAGTCTGTTTTAGCTATGTGGTAAATGGAAATAAGTTTTCTGAAATCTGAAATATTTAGTTGTTTGTTGTATTCCATCCTCTATCATTTACTAGTGTATTTCTAAAGGACGATGGTGTCATTGTCACTCCAGGAGGTCGAAAAGGATGTGAAAACAAGGACTAAAGGTGAGATGGGAGCAGCCAAAACCTTATGTTCCCCGTTCGAGCAGCCTCCTCTTCCCGAAGGTTACTAAATTCATGTCCAAATCTCTGTATTCATTTTGCATGTTAATATGAGTTTATACCAACTTGTGGGATCTTGGGAAACTTGGTAACAACTCTTTTGGTTCCTTGAATTTACTATGATGTTTGTGCTCAGGTACCAAATGTTTTGCTTCTGGGAAGCCTGCAAAGAAATGGAGCTATTGGGGCCGAAGCTACTAAAGCTAGTCAGCTCATATTTTTCTCCTACGCTTCGTCTAATTACACGATGTCGGAAATCAGCAAGAGTTCTCGAGTTCAGGGGCATTCAATTCAGTGTTCATCATAATTTCTGATAGTCACTTGTATTAGATGAAGAAAAGCATTCACTAAGCAAAGCAAGTTTAACTTGAAATGTTTTCTCTCTTTGCATACTTGATTTTTTTTGGTTGAAAATTTATGGGTTTTTTTTCCTTTCAATCTTGGTTTTCAGTTCTGTTAAACTAGGCCAATTCTTGACAGTTTCTAAATTATATACTTCGTAACTTGAATATAGTAACATCATGTCAGTTAGGG

mRNA sequence

GCATATATGAAGAATACCTGGCAATTCCTGTTATCAAAGGCAAGAAGAGTGAGATGGAGAAGTTTGCTGGTGGTCTTTACACCACAAGCGTTGAGGCATTTATCCCAAACACTGGTCGTGGTATTCAAGGTGCAACTTCACATTGTTTGGGTCAAAATTTTGCAAAAATGTTTGAAATCAACTTTGAAAATGAAAAGGGGGAGAAAGCTATGGTCTGGCAAAATTCGTGGGCCTATAGTACCAGAACGATTGGTGTGATGGTTATGGTTCACGGTGATGACAAGGGATTGGTGATGCCTCCCAAAGTTGCATCAGTGCAAGTCATTATAGTTCCTGTTCCCTACAAAGATGCAGACACTCGAGGAATTTTTGATGTTTGTTCTGCCACTTCAGATGCATTGTCCAAAGCAGGAATTCGTGCAGAGGTAGATATTAGGGAGAACTATTCTCCTGGATGGAAGTACTCCCACTGGGAAATGAAAGGTGTTCCACTCAGGATTGAAATAGGGCCTAAGGACTTGGCGAACAATCAGGTACGTGCAGTTCGCCGTGATAATTCTGCAAAGAAGGACATACCTAGGGCTTTGTTGGTTGAACAAGTGAAAGAATTGCTAGAAAGTATTCAACAAAGCCTTTTTGATGCGGCAAAAGAAAAACGAGATGCATGCATTCAGGTTGTTAATACATGGGAGGAGTTTACTGAAGCCCTCGGTCAGAAGAAAATGATATTAGCTCCATGGTGTAATGAAGAGGAGGTCGAAAAGGATGTGAAAACAAGGACTAAAGGTGAGATGGGAGCAGCCAAAACCTTATGTTCCCCGTTCGAGCAGCCTCCTCTTCCCGAAGGTACCAAATGTTTTGCTTCTGGGAAGCCTGCAAAGAAATGGAGCTATTGGGGCCGAAGCTACTAAAGCTAGTCAGCTCATATTTTTCTCCTACGCTTCGTCTAATTACACGATGTCGGAAATCAGCAAGAGTTCTCGAGTTCAGGGGCATTCAATTCAGTGTTCATCATAATTTCTGATAGTCACTTGTATTAGATGAAGAAAAGCATTCACTAAGCAAAGCAAGTTTAACTTGAAATGTTTTCTCTCTTTGCATACTTGATTTTTTTTGGTTGAAAATTTATGGGTTTTTTTTCCTTTCAATCTTGGTTTTCAGTTCTGTTAAACTAGGCCAATTCTTGACAGTTTCTAAATTATATACTTCGTAACTTGAATATAGTAACATCATGTCAGTTAGGG

Coding sequence (CDS)

ATGGAGAAGTTTGCTGGTGGTCTTTACACCACAAGCGTTGAGGCATTTATCCCAAACACTGGTCGTGGTATTCAAGGTGCAACTTCACATTGTTTGGGTCAAAATTTTGCAAAAATGTTTGAAATCAACTTTGAAAATGAAAAGGGGGAGAAAGCTATGGTCTGGCAAAATTCGTGGGCCTATAGTACCAGAACGATTGGTGTGATGGTTATGGTTCACGGTGATGACAAGGGATTGGTGATGCCTCCCAAAGTTGCATCAGTGCAAGTCATTATAGTTCCTGTTCCCTACAAAGATGCAGACACTCGAGGAATTTTTGATGTTTGTTCTGCCACTTCAGATGCATTGTCCAAAGCAGGAATTCGTGCAGAGGTAGATATTAGGGAGAACTATTCTCCTGGATGGAAGTACTCCCACTGGGAAATGAAAGGTGTTCCACTCAGGATTGAAATAGGGCCTAAGGACTTGGCGAACAATCAGGTACGTGCAGTTCGCCGTGATAATTCTGCAAAGAAGGACATACCTAGGGCTTTGTTGGTTGAACAAGTGAAAGAATTGCTAGAAAGTATTCAACAAAGCCTTTTTGATGCGGCAAAAGAAAAACGAGATGCATGCATTCAGGTTGTTAATACATGGGAGGAGTTTACTGAAGCCCTCGGTCAGAAGAAAATGATATTAGCTCCATGGTGTAATGAAGAGGAGGTCGAAAAGGATGTGAAAACAAGGACTAAAGGTGAGATGGGAGCAGCCAAAACCTTATGTTCCCCGTTCGAGCAGCCTCCTCTTCCCGAAGGTACCAAATGTTTTGCTTCTGGGAAGCCTGCAAAGAAATGGAGCTATTGGGGCCGAAGCTACTAA

Protein sequence

MEKFAGGLYTTSVEAFIPNTGRGIQGATSHCLGQNFAKMFEINFENEKGEKAMVWQNSWAYSTRTIGVMVMVHGDDKGLVMPPKVASVQVIIVPVPYKDADTRGIFDVCSATSDALSKAGIRAEVDIRENYSPGWKYSHWEMKGVPLRIEIGPKDLANNQVRAVRRDNSAKKDIPRALLVEQVKELLESIQQSLFDAAKEKRDACIQVVNTWEEFTEALGQKKMILAPWCNEEEVEKDVKTRTKGEMGAAKTLCSPFEQPPLPEGTKCFASGKPAKKWSYWGRSY

Homology

[Close]

BLAST of Cmc09g0251221 vs. NCBI nr
Match: KAA0050272.1 (proline--tRNA ligase [Cucumis melo var. makuwa] >TYJ98135.1 proline--tRNA ligase [Cucumis melo var. makuwa])

HSP 1 Score: 586.6 bits (1511), Expect = 1.1e-163
Identity = 285/285 (100.00%), Postives = 285/285 (100.00%), Query Frame = 0


Query: 1   MEKFAGGLYTTSVEAFIPNTGRGIQGATSHCLGQNFAKMFEINFENEKGEKAMVWQNSWA 60
           MEKFAGGLYTTSVEAFIPNTGRGIQGATSHCLGQNFAKMFEINFENEKGEKAMVWQNSWA
Sbjct: 228 MEKFAGGLYTTSVEAFIPNTGRGIQGATSHCLGQNFAKMFEINFENEKGEKAMVWQNSWA 287

Query: 61  YSTRTIGVMVMVHGDDKGLVMPPKVASVQVIIVPVPYKDADTRGIFDVCSATSDALSKAG 120
           YSTRTIGVMVMVHGDDKGLVMPPKVASVQVIIVPVPYKDADTRGIFDVCSATSDALSKAG
Sbjct: 288 YSTRTIGVMVMVHGDDKGLVMPPKVASVQVIIVPVPYKDADTRGIFDVCSATSDALSKAG 347

Query: 121 IRAEVDIRENYSPGWKYSHWEMKGVPLRIEIGPKDLANNQVRAVRRDNSAKKDIPRALLV 180
           IRAEVDIRENYSPGWKYSHWEMKGVPLRIEIGPKDLANNQVRAVRRDNSAKKDIPRALLV
Sbjct: 348 IRAEVDIRENYSPGWKYSHWEMKGVPLRIEIGPKDLANNQVRAVRRDNSAKKDIPRALLV 407

Query: 181 EQVKELLESIQQSLFDAAKEKRDACIQVVNTWEEFTEALGQKKMILAPWCNEEEVEKDVK 240
           EQVKELLESIQQSLFDAAKEKRDACIQVVNTWEEFTEALGQKKMILAPWCNEEEVEKDVK
Sbjct: 408 EQVKELLESIQQSLFDAAKEKRDACIQVVNTWEEFTEALGQKKMILAPWCNEEEVEKDVK 467

Query: 241 TRTKGEMGAAKTLCSPFEQPPLPEGTKCFASGKPAKKWSYWGRSY 286
           TRTKGEMGAAKTLCSPFEQPPLPEGTKCFASGKPAKKWSYWGRSY
Sbjct: 468 TRTKGEMGAAKTLCSPFEQPPLPEGTKCFASGKPAKKWSYWGRSY 512

[Close]

BLAST of Cmc09g0251221 vs. NCBI nr
Match: XP_008466343.1 (PREDICTED: proline--tRNA ligase, cytoplasmic isoform X4 [Cucumis melo])

HSP 1 Score: 585.1 bits (1507), Expect = 3.3e-163
Identity = 284/285 (99.65%), Postives = 284/285 (99.65%), Query Frame = 0


Query: 1   MEKFAGGLYTTSVEAFIPNTGRGIQGATSHCLGQNFAKMFEINFENEKGEKAMVWQNSWA 60
           MEKFAGGLYTTSVEAFIPNTGRGIQGATSHCLGQNFAKMFEINFENEKGEKAMVWQNSWA
Sbjct: 177 MEKFAGGLYTTSVEAFIPNTGRGIQGATSHCLGQNFAKMFEINFENEKGEKAMVWQNSWA 236

Query: 61  YSTRTIGVMVMVHGDDKGLVMPPKVASVQVIIVPVPYKDADTRGIFDVCSATSDALSKAG 120
           YSTRTIGVMVMVHGDDKGLVMPPKVASVQVIIVPVPYKDADTRGIFD CSATSDALSKAG
Sbjct: 237 YSTRTIGVMVMVHGDDKGLVMPPKVASVQVIIVPVPYKDADTRGIFDACSATSDALSKAG 296

Query: 121 IRAEVDIRENYSPGWKYSHWEMKGVPLRIEIGPKDLANNQVRAVRRDNSAKKDIPRALLV 180
           IRAEVDIRENYSPGWKYSHWEMKGVPLRIEIGPKDLANNQVRAVRRDNSAKKDIPRALLV
Sbjct: 297 IRAEVDIRENYSPGWKYSHWEMKGVPLRIEIGPKDLANNQVRAVRRDNSAKKDIPRALLV 356

Query: 181 EQVKELLESIQQSLFDAAKEKRDACIQVVNTWEEFTEALGQKKMILAPWCNEEEVEKDVK 240
           EQVKELLESIQQSLFDAAKEKRDACIQVVNTWEEFTEALGQKKMILAPWCNEEEVEKDVK
Sbjct: 357 EQVKELLESIQQSLFDAAKEKRDACIQVVNTWEEFTEALGQKKMILAPWCNEEEVEKDVK 416

Query: 241 TRTKGEMGAAKTLCSPFEQPPLPEGTKCFASGKPAKKWSYWGRSY 286
           TRTKGEMGAAKTLCSPFEQPPLPEGTKCFASGKPAKKWSYWGRSY
Sbjct: 417 TRTKGEMGAAKTLCSPFEQPPLPEGTKCFASGKPAKKWSYWGRSY 461

[Close]

BLAST of Cmc09g0251221 vs. NCBI nr
Match: XP_008466310.1 (PREDICTED: proline--tRNA ligase, cytoplasmic isoform X1 [Cucumis melo] >XP_008466350.1 PREDICTED: proline--tRNA ligase, cytoplasmic isoform X1 [Cucumis melo] >XP_016898851.1 PREDICTED: proline--tRNA ligase, cytoplasmic isoform X1 [Cucumis melo])

HSP 1 Score: 585.1 bits (1507), Expect = 3.3e-163
Identity = 284/285 (99.65%), Postives = 284/285 (99.65%), Query Frame = 0


Query: 1   MEKFAGGLYTTSVEAFIPNTGRGIQGATSHCLGQNFAKMFEINFENEKGEKAMVWQNSWA 60
           MEKFAGGLYTTSVEAFIPNTGRGIQGATSHCLGQNFAKMFEINFENEKGEKAMVWQNSWA
Sbjct: 227 MEKFAGGLYTTSVEAFIPNTGRGIQGATSHCLGQNFAKMFEINFENEKGEKAMVWQNSWA 286

Query: 61  YSTRTIGVMVMVHGDDKGLVMPPKVASVQVIIVPVPYKDADTRGIFDVCSATSDALSKAG 120
           YSTRTIGVMVMVHGDDKGLVMPPKVASVQVIIVPVPYKDADTRGIFD CSATSDALSKAG
Sbjct: 287 YSTRTIGVMVMVHGDDKGLVMPPKVASVQVIIVPVPYKDADTRGIFDACSATSDALSKAG 346

Query: 121 IRAEVDIRENYSPGWKYSHWEMKGVPLRIEIGPKDLANNQVRAVRRDNSAKKDIPRALLV 180
           IRAEVDIRENYSPGWKYSHWEMKGVPLRIEIGPKDLANNQVRAVRRDNSAKKDIPRALLV
Sbjct: 347 IRAEVDIRENYSPGWKYSHWEMKGVPLRIEIGPKDLANNQVRAVRRDNSAKKDIPRALLV 406

Query: 181 EQVKELLESIQQSLFDAAKEKRDACIQVVNTWEEFTEALGQKKMILAPWCNEEEVEKDVK 240
           EQVKELLESIQQSLFDAAKEKRDACIQVVNTWEEFTEALGQKKMILAPWCNEEEVEKDVK
Sbjct: 407 EQVKELLESIQQSLFDAAKEKRDACIQVVNTWEEFTEALGQKKMILAPWCNEEEVEKDVK 466

Query: 241 TRTKGEMGAAKTLCSPFEQPPLPEGTKCFASGKPAKKWSYWGRSY 286
           TRTKGEMGAAKTLCSPFEQPPLPEGTKCFASGKPAKKWSYWGRSY
Sbjct: 467 TRTKGEMGAAKTLCSPFEQPPLPEGTKCFASGKPAKKWSYWGRSY 511

[Close]

BLAST of Cmc09g0251221 vs. NCBI nr
Match: XP_022979761.1 (proline--tRNA ligase, cytoplasmic-like [Cucurbita maxima])

HSP 1 Score: 560.1 bits (1442), Expect = 1.1e-155
Identity = 270/285 (94.74%), Postives = 276/285 (96.84%), Query Frame = 0


Query: 1   MEKFAGGLYTTSVEAFIPNTGRGIQGATSHCLGQNFAKMFEINFENEKGEKAMVWQNSWA 60
           MEKFAGGLYTTSVEAFIPNTGRGIQGATSHCLGQNFAKMFEINFENEKGEKAMVWQNSWA
Sbjct: 226 MEKFAGGLYTTSVEAFIPNTGRGIQGATSHCLGQNFAKMFEINFENEKGEKAMVWQNSWA 285

Query: 61  YSTRTIGVMVMVHGDDKGLVMPPKVASVQVIIVPVPYKDADTRGIFDVCSATSDALSKAG 120
           YSTRTIGVMVMVHGDDKGLV+PPKVASVQVIIVPVPYKDADT+GIFD CSAT D LSKAG
Sbjct: 286 YSTRTIGVMVMVHGDDKGLVLPPKVASVQVIIVPVPYKDADTQGIFDACSATLDTLSKAG 345

Query: 121 IRAEVDIRENYSPGWKYSHWEMKGVPLRIEIGPKDLANNQVRAVRRDNSAKKDIPRALLV 180
           IRAEVD R+NYSPGWKYSHWEMKGVPLRIEIGPKDLA+NQVRAVRRDNS KKDIPRA LV
Sbjct: 346 IRAEVDSRDNYSPGWKYSHWEMKGVPLRIEIGPKDLASNQVRAVRRDNSGKKDIPRASLV 405

Query: 181 EQVKELLESIQQSLFDAAKEKRDACIQVVNTWEEFTEALGQKKMILAPWCNEEEVEKDVK 240
           EQVKELLESIQQSLFDAAKEKRD CIQV+NTWEEFTEAL QKKMILAPWC+EEEVEKDVK
Sbjct: 406 EQVKELLESIQQSLFDAAKEKRDVCIQVINTWEEFTEALSQKKMILAPWCDEEEVEKDVK 465

Query: 241 TRTKGEMGAAKTLCSPFEQPPLPEGTKCFASGKPAKKWSYWGRSY 286
           TRTKGEMGAAKTLCSPFEQP LPEGTKCFASGKPAKKWSYWGRSY
Sbjct: 466 TRTKGEMGAAKTLCSPFEQPALPEGTKCFASGKPAKKWSYWGRSY 510

[Close]

BLAST of Cmc09g0251221 vs. NCBI nr
Match: XP_038907039.1 (proline--tRNA ligase, cytoplasmic-like isoform X2 [Benincasa hispida])

HSP 1 Score: 560.1 bits (1442), Expect = 1.1e-155
Identity = 270/285 (94.74%), Postives = 276/285 (96.84%), Query Frame = 0


Query: 1   MEKFAGGLYTTSVEAFIPNTGRGIQGATSHCLGQNFAKMFEINFENEKGEKAMVWQNSWA 60
           MEKFAGGLYTTSVEAFIPNTGRGIQGATSHCLGQNFAKMFEINFENEKGEKAMVWQNSWA
Sbjct: 177 MEKFAGGLYTTSVEAFIPNTGRGIQGATSHCLGQNFAKMFEINFENEKGEKAMVWQNSWA 236

Query: 61  YSTRTIGVMVMVHGDDKGLVMPPKVASVQVIIVPVPYKDADTRGIFDVCSATSDALSKAG 120
           YSTRTIGVMVMVHGDDKGLV+PPKVASVQVIIVPVPYKDADT+GIFD CSAT D LS+AG
Sbjct: 237 YSTRTIGVMVMVHGDDKGLVLPPKVASVQVIIVPVPYKDADTQGIFDACSATLDTLSEAG 296

Query: 121 IRAEVDIRENYSPGWKYSHWEMKGVPLRIEIGPKDLANNQVRAVRRDNSAKKDIPRALLV 180
           IRAEVD R+NYSPGWKYSHWEMKGVPLRIEIGPKDLANNQVRAVRRDNS KKDIPR  LV
Sbjct: 297 IRAEVDSRDNYSPGWKYSHWEMKGVPLRIEIGPKDLANNQVRAVRRDNSGKKDIPRDSLV 356

Query: 181 EQVKELLESIQQSLFDAAKEKRDACIQVVNTWEEFTEALGQKKMILAPWCNEEEVEKDVK 240
           EQVKELLESIQQSLFDAAKEKRD CIQV+NTWEEFTEALGQKKMILAPWC+EEEVEKDVK
Sbjct: 357 EQVKELLESIQQSLFDAAKEKRDKCIQVINTWEEFTEALGQKKMILAPWCDEEEVEKDVK 416

Query: 241 TRTKGEMGAAKTLCSPFEQPPLPEGTKCFASGKPAKKWSYWGRSY 286
           TRTKGEMGAAKTLCSPFEQP LPEGTKCFASGKPAKKWSYWGRSY
Sbjct: 417 TRTKGEMGAAKTLCSPFEQPALPEGTKCFASGKPAKKWSYWGRSY 461

[Close]

BLAST of Cmc09g0251221 vs. ExPASy Swiss-Prot
Match: Q9M1R2 (Proline--tRNA ligase, cytoplasmic OS=Arabidopsis thaliana OX=3702 GN=At3g62120 PE=1 SV=1)

HSP 1 Score: 493.8 bits (1270), Expect = 1.3e-138
Identity = 226/284 (79.58%), Postives = 258/284 (90.85%), Query Frame = 0


Query: 2   EKFAGGLYTTSVEAFIPNTGRGIQGATSHCLGQNFAKMFEINFENEKGEKAMVWQNSWAY 61
           EKFAGGLYTTSVEAFIPNTGRG+QGATSHCLGQNFAKMFEINFENEK E  MVWQNSWAY
Sbjct: 247 EKFAGGLYTTSVEAFIPNTGRGVQGATSHCLGQNFAKMFEINFENEKAETEMVWQNSWAY 306

Query: 62  STRTIGVMVMVHGDDKGLVMPPKVASVQVIIVPVPYKDADTRGIFDVCSATSDALSKAGI 121
           STRTIGVM+M HGDDKGLV+PPKVASVQV+++PVPYKDA+T+GI+D C+AT+ AL +AGI
Sbjct: 307 STRTIGVMIMTHGDDKGLVLPPKVASVQVVVIPVPYKDANTQGIYDACTATASALCEAGI 366

Query: 122 RAEVDIRENYSPGWKYSHWEMKGVPLRIEIGPKDLANNQVRAVRRDNSAKKDIPRALLVE 181
           RAE D+R+NYSPGWKYS WEMKGVPLRIEIGP+DL N+QVR VRRDN  K+DIPR  LVE
Sbjct: 367 RAEEDLRDNYSPGWKYSDWEMKGVPLRIEIGPRDLENDQVRTVRRDNGVKEDIPRGSLVE 426

Query: 182 QVKELLESIQQSLFDAAKEKRDACIQVVNTWEEFTEALGQKKMILAPWCNEEEVEKDVKT 241
            VKELLE IQQ++++ AK+KR+AC+Q V TW+EF +AL +KK+ILAPWC+EEEVE+DVK 
Sbjct: 427 HVKELLEKIQQNMYEVAKQKREACVQEVKTWDEFIKALNEKKLILAPWCDEEEVERDVKA 486

Query: 242 RTKGEMGAAKTLCSPFEQPPLPEGTKCFASGKPAKKWSYWGRSY 286
           RTKGE GAAKTLCSPF+QP LPEGT CFASGKPAKKW+YWGRSY
Sbjct: 487 RTKGETGAAKTLCSPFDQPELPEGTLCFASGKPAKKWTYWGRSY 530

[Close]

BLAST of Cmc09g0251221 vs. ExPASy Swiss-Prot
Match: Q8I5R7 (Proline--tRNA ligase OS=Plasmodium falciparum (isolate 3D7) OX=36329 GN=proRS PE=1 SV=1)

HSP 1 Score: 286.2 bits (731), Expect = 4.1e-76
Identity = 142/295 (48.14%), Postives = 191/295 (64.75%), Query Frame = 0


Query: 2   EKFAGGLYTTSVEAFIPNTGRGIQGATSHCLGQNFAKMFEINFENEKGEKAMVWQNSWAY 61
           EKF G  +T++ EAFI   GR IQ ATSH LG NFAKMF+I FE+E   K  V Q SW  
Sbjct: 452 EKFGGANFTSTAEAFISENGRAIQAATSHYLGTNFAKMFKIEFEDENEVKQYVHQTSWGC 511

Query: 62  STRTIGVMVMVHGDDKGLVMPPKVASVQVIIVPVPYKDADTRGIFDVCSATSDALSKAGI 121
           +TR+IG+M+M HGDDKGLV+PP V+  +V+IVP+ YK  D   I   C      L  A I
Sbjct: 512 TTRSIGIMIMTHGDDKGLVLPPNVSKYKVVIVPIFYKTTDENAIHSYCKDIEKILKNAQI 571

Query: 122 RAEVDIRENYSPGWKYSHWEMKGVPLRIEIGPKDLANNQVRAVRRDNSAKKDIPRALLVE 181
               D R +YSPG+K++HWE++G+P+RIE+GPKDL NN    VRRDN+ K ++ +  ++ 
Sbjct: 572 NCVYDDRASYSPGYKFNHWELRGIPIRIEVGPKDLQNNSCVIVRRDNNEKCNVKKESVLL 631

Query: 182 QVKELLESIQQSLFDAAKEKRDACIQVVNTWEEFTEALGQKKMILAPWC----NEEEVEK 241
           + +++L  I ++LF  AK+K D  I  V ++ E   AL +KKM+LAPWC     EEE++K
Sbjct: 632 ETQQMLVDIHKNLFLKAKKKLDDSIVQVTSFSEVMNALNKKKMVLAPWCEDIATEEEIKK 691

Query: 242 DVK----TRTKGE---MGAAKTLCSPFEQPPLPEGTKCFASGKPAKKWSYWGRSY 286
           + +     +T  E    GA K LC P +QPP+P   KCF SGKPAK+W  +GRSY
Sbjct: 692 ETQRLSLNQTNSETTLSGAMKPLCIPLDQPPMPPNMKCFWSGKPAKRWCLFGRSY 746

[Close]

BLAST of Cmc09g0251221 vs. ExPASy Swiss-Prot
Match: O60155 (Putative proline--tRNA ligase C19C7.06 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=prs1 PE=3 SV=1)

HSP 1 Score: 285.4 bits (729), Expect = 7.0e-76
Identity = 154/313 (49.20%), Postives = 192/313 (61.34%), Query Frame = 0


Query: 2   EKFAGGLYTTSVEAFIPNTGRGIQGATSHCLGQNFAKMFEINFENEKGE--------KAM 61
           EKFAGG++TT+VE +IP TGRGIQGATSHCLGQNF+KMF I  E+   E        K  
Sbjct: 405 EKFAGGMFTTTVEGYIPTTGRGIQGATSHCLGQNFSKMFNIVVEDPNAEIGPTGERPKLF 464

Query: 62  VWQNSWAYSTRTIGVMVMVHGDDKGLVMPPKVASVQVIIVPV----PYKDADTRGIFDVC 121
           VWQNSW  STRTIGV VMVHGDDKGL +PP +A VQ ++VP        D +   I   C
Sbjct: 465 VWQNSWGLSTRTIGVAVMVHGDDKGLKLPPAIALVQSVVVPCGITNKTTDQERNEIEGFC 524

Query: 122 SATSDALSKAGIRAEVDIRENYSPGWKYSHWEMKGVPLRIEIGPKDLANNQVRAVRRDNS 181
           S  +D L+ A IR E D+R  Y+PG+K+SHWEMKGVPLR+E GP D   NQV AVRRD  
Sbjct: 525 SKLADRLNAADIRTEADLRA-YTPGYKFSHWEMKGVPLRLEYGPNDAKKNQVTAVRRDTF 584

Query: 182 AKKDIPRALLVEQVKELLESIQQSLFDAAKEKRDACIQVVNTWEEFTEALGQKKMILAPW 241
            K  +P   L + V +LL  IQ ++++ AK +RDA +  V  W +F  AL +K +++ PW
Sbjct: 585 EKIPVPLNNLEKGVSDLLAKIQTNMYETAKAERDAHVVKVKEWADFVPALNKKNIVMIPW 644

Query: 242 CNEEEVEKDVKTRT------------KGEMGAAKTLCSPFEQP----PLPEG-TKCFASG 286
           CN  E EK++K  +            K     AK+LC P EQP     + EG TKC   G
Sbjct: 645 CNTTECEKEIKKNSARQVNGDEPEDEKAPSMGAKSLCIPLEQPSGEDAIIEGTTKCAGCG 704

[Close]

BLAST of Cmc09g0251221 vs. ExPASy Swiss-Prot
Match: P28668 (Bifunctional glutamate/proline--tRNA ligase OS=Drosophila melanogaster OX=7227 GN=GluProRS PE=1 SV=2)

HSP 1 Score: 269.2 bits (687), Expect = 5.2e-71
Identity = 148/301 (49.17%), Postives = 197/301 (65.45%), Query Frame = 0


Query: 2    EKFAGGLYTTSVEAFIPNTGRGIQGATSHCLGQNFAKMFEINFEN-EKGEKAMVWQNSWA 61
            EKFAGG YTT+VEAFI  +GR IQGATSH LGQNF+KMFEI +E+ E  +K  V+QNSW 
Sbjct: 1415 EKFAGGDYTTTVEAFISASGRAIQGATSHHLGQNFSKMFEIVYEDPETQQKKYVYQNSWG 1474

Query: 62   YSTRTIGVMVMVHGDDKGLVMPPKVASVQVIIVP----VPYKDADTRGIFDVCSATSDAL 121
             +TRTIGVM+MVH D++GLV+PP VA +Q I+VP    V  KD +   + D C A    L
Sbjct: 1475 ITTRTIGVMIMVHADNQGLVLPPHVACIQAIVVPCGITVNTKDDERAQLLDACKALEKRL 1534

Query: 122  SKAGIRAEVDIRENYSPGWKYSHWEMKGVPLRIEIGPKDLANNQVRAVRRDNSAKKDIPR 181
               G+R E D R+NYSPGWK++HWE+KGVPLR+E+GPKDL   Q+ AVRRD   K  IP 
Sbjct: 1535 VGGGVRCEGDYRDNYSPGWKFNHWELKGVPLRLEVGPKDLKAQQLVAVRRDTVEKITIPL 1594

Query: 182  ALLVEQVKELLESIQQSLFDAAKEKRDACIQVVNTWEEFTEALGQKKMILAPWCNEEEVE 241
            A + +++  LLE+I +S+ + A+E   +  + V  W +F   L QK ++LAP+C E   E
Sbjct: 1595 ADVEKKIPALLETIHESMLNKAQEDMTSHTKKVTNWTDFCGFLEQKNILLAPFCGEISCE 1654

Query: 242  KDVKTRT----KGEMGA----AKTLCSPFEQP-PLPEGTKCF---ASGKPAKKWSYWGRS 286
              +K  +    + E GA    AK+LC PF+QP P+    KC     + KP K ++ +GRS
Sbjct: 1655 DKIKADSARGEEAEPGAPAMGAKSLCIPFDQPAPIAASDKCINPSCTNKP-KFYTLFGRS 1714

[Close]

BLAST of Cmc09g0251221 vs. ExPASy Swiss-Prot
Match: P38708 (Putative proline--tRNA ligase YHR020W OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=YHR020W PE=1 SV=1)

HSP 1 Score: 267.7 bits (683), Expect = 1.5e-70
Identity = 140/304 (46.05%), Postives = 186/304 (61.18%), Query Frame = 0


Query: 2   EKFAGGLYTTSVEAFIPNTGRGIQGATSHCLGQNFAKMFEINFENEKGE---KAMVWQNS 61
           EKFAGG +TT+ E +IP TGRGIQGATSH LGQNF+KMF ++ EN  G    K   +QNS
Sbjct: 385 EKFAGGDFTTTCEGYIPQTGRGIQGATSHHLGQNFSKMFNLSVENPLGSDHPKIFAYQNS 444

Query: 62  WAYSTRTIGVMVMVHGDDKGLVMPPKVASVQVIIVPV----PYKDADTRGIFDVCSATSD 121
           W  STR IGVMVM+H D+KGLV+PP+V+  Q +++PV       +   + I +   +   
Sbjct: 445 WGLSTRVIGVMVMIHSDNKGLVIPPRVSQFQSVVIPVGITKKTSEEQRKHIHETARSVES 504

Query: 122 ALSKAGIRAEVDIRENYSPGWKYSHWEMKGVPLRIEIGPKDLANNQVRAVRRDNSAKKDI 181
            L K GIRA  D  +NY+PGWK+S +E+KG+P+RIE+GPKD+  NQV  VRR++S K  +
Sbjct: 505 RLKKVGIRAFGDYNDNYTPGWKFSQYELKGIPIRIELGPKDIEKNQVVVVRRNDSKKYVV 564

Query: 182 PRALLVEQVKELLESIQQSLFDAAKEKRDACIQVVNTWEEFTEALGQKKMILAPWCNEEE 241
               L  ++ E+LE +Q  LF  AKE  D    +VN W  F  AL +K +ILAPWC   E
Sbjct: 565 SFDELEARIPEILEEMQGDLFKKAKELFDTHRVIVNEWSGFVPALNKKNVILAPWCGVME 624

Query: 242 VEKDVKTRT-------------KGEMGAAKTLCSPFEQPPLPEGTKCFASGKPAKKWSYW 286
            E+D+K  +             K     AK+LC PF+QP L EG KC    + A  +  +
Sbjct: 625 CEEDIKESSAKKDDGEEFEEDDKAPSMGAKSLCIPFDQPVLNEGQKCIKCERIAVNYCMF 684

[Close]

BLAST of Cmc09g0251221 vs. ExPASy TrEMBL
Match: A0A5A7U355 (Prolyl-tRNA synthetase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold180G00130 PE=3 SV=1)

HSP 1 Score: 586.6 bits (1511), Expect = 5.4e-164
Identity = 285/285 (100.00%), Postives = 285/285 (100.00%), Query Frame = 0


Query: 1   MEKFAGGLYTTSVEAFIPNTGRGIQGATSHCLGQNFAKMFEINFENEKGEKAMVWQNSWA 60
           MEKFAGGLYTTSVEAFIPNTGRGIQGATSHCLGQNFAKMFEINFENEKGEKAMVWQNSWA
Sbjct: 228 MEKFAGGLYTTSVEAFIPNTGRGIQGATSHCLGQNFAKMFEINFENEKGEKAMVWQNSWA 287

Query: 61  YSTRTIGVMVMVHGDDKGLVMPPKVASVQVIIVPVPYKDADTRGIFDVCSATSDALSKAG 120
           YSTRTIGVMVMVHGDDKGLVMPPKVASVQVIIVPVPYKDADTRGIFDVCSATSDALSKAG
Sbjct: 288 YSTRTIGVMVMVHGDDKGLVMPPKVASVQVIIVPVPYKDADTRGIFDVCSATSDALSKAG 347

Query: 121 IRAEVDIRENYSPGWKYSHWEMKGVPLRIEIGPKDLANNQVRAVRRDNSAKKDIPRALLV 180
           IRAEVDIRENYSPGWKYSHWEMKGVPLRIEIGPKDLANNQVRAVRRDNSAKKDIPRALLV
Sbjct: 348 IRAEVDIRENYSPGWKYSHWEMKGVPLRIEIGPKDLANNQVRAVRRDNSAKKDIPRALLV 407

Query: 181 EQVKELLESIQQSLFDAAKEKRDACIQVVNTWEEFTEALGQKKMILAPWCNEEEVEKDVK 240
           EQVKELLESIQQSLFDAAKEKRDACIQVVNTWEEFTEALGQKKMILAPWCNEEEVEKDVK
Sbjct: 408 EQVKELLESIQQSLFDAAKEKRDACIQVVNTWEEFTEALGQKKMILAPWCNEEEVEKDVK 467

Query: 241 TRTKGEMGAAKTLCSPFEQPPLPEGTKCFASGKPAKKWSYWGRSY 286
           TRTKGEMGAAKTLCSPFEQPPLPEGTKCFASGKPAKKWSYWGRSY
Sbjct: 468 TRTKGEMGAAKTLCSPFEQPPLPEGTKCFASGKPAKKWSYWGRSY 512

[Close]

BLAST of Cmc09g0251221 vs. ExPASy TrEMBL
Match: A0A1S4DS87 (Prolyl-tRNA synthetase OS=Cucumis melo OX=3656 GN=LOC103503756 PE=3 SV=1)

HSP 1 Score: 585.1 bits (1507), Expect = 1.6e-163
Identity = 284/285 (99.65%), Postives = 284/285 (99.65%), Query Frame = 0


Query: 1   MEKFAGGLYTTSVEAFIPNTGRGIQGATSHCLGQNFAKMFEINFENEKGEKAMVWQNSWA 60
           MEKFAGGLYTTSVEAFIPNTGRGIQGATSHCLGQNFAKMFEINFENEKGEKAMVWQNSWA
Sbjct: 227 MEKFAGGLYTTSVEAFIPNTGRGIQGATSHCLGQNFAKMFEINFENEKGEKAMVWQNSWA 286

Query: 61  YSTRTIGVMVMVHGDDKGLVMPPKVASVQVIIVPVPYKDADTRGIFDVCSATSDALSKAG 120
           YSTRTIGVMVMVHGDDKGLVMPPKVASVQVIIVPVPYKDADTRGIFD CSATSDALSKAG
Sbjct: 287 YSTRTIGVMVMVHGDDKGLVMPPKVASVQVIIVPVPYKDADTRGIFDACSATSDALSKAG 346

Query: 121 IRAEVDIRENYSPGWKYSHWEMKGVPLRIEIGPKDLANNQVRAVRRDNSAKKDIPRALLV 180
           IRAEVDIRENYSPGWKYSHWEMKGVPLRIEIGPKDLANNQVRAVRRDNSAKKDIPRALLV
Sbjct: 347 IRAEVDIRENYSPGWKYSHWEMKGVPLRIEIGPKDLANNQVRAVRRDNSAKKDIPRALLV 406

Query: 181 EQVKELLESIQQSLFDAAKEKRDACIQVVNTWEEFTEALGQKKMILAPWCNEEEVEKDVK 240
           EQVKELLESIQQSLFDAAKEKRDACIQVVNTWEEFTEALGQKKMILAPWCNEEEVEKDVK
Sbjct: 407 EQVKELLESIQQSLFDAAKEKRDACIQVVNTWEEFTEALGQKKMILAPWCNEEEVEKDVK 466

Query: 241 TRTKGEMGAAKTLCSPFEQPPLPEGTKCFASGKPAKKWSYWGRSY 286
           TRTKGEMGAAKTLCSPFEQPPLPEGTKCFASGKPAKKWSYWGRSY
Sbjct: 467 TRTKGEMGAAKTLCSPFEQPPLPEGTKCFASGKPAKKWSYWGRSY 511

[Close]

BLAST of Cmc09g0251221 vs. ExPASy TrEMBL
Match: A0A1S3CR78 (Prolyl-tRNA synthetase OS=Cucumis melo OX=3656 GN=LOC103503756 PE=3 SV=1)

HSP 1 Score: 585.1 bits (1507), Expect = 1.6e-163
Identity = 284/285 (99.65%), Postives = 284/285 (99.65%), Query Frame = 0


Query: 1   MEKFAGGLYTTSVEAFIPNTGRGIQGATSHCLGQNFAKMFEINFENEKGEKAMVWQNSWA 60
           MEKFAGGLYTTSVEAFIPNTGRGIQGATSHCLGQNFAKMFEINFENEKGEKAMVWQNSWA
Sbjct: 177 MEKFAGGLYTTSVEAFIPNTGRGIQGATSHCLGQNFAKMFEINFENEKGEKAMVWQNSWA 236

Query: 61  YSTRTIGVMVMVHGDDKGLVMPPKVASVQVIIVPVPYKDADTRGIFDVCSATSDALSKAG 120
           YSTRTIGVMVMVHGDDKGLVMPPKVASVQVIIVPVPYKDADTRGIFD CSATSDALSKAG
Sbjct: 237 YSTRTIGVMVMVHGDDKGLVMPPKVASVQVIIVPVPYKDADTRGIFDACSATSDALSKAG 296

Query: 121 IRAEVDIRENYSPGWKYSHWEMKGVPLRIEIGPKDLANNQVRAVRRDNSAKKDIPRALLV 180
           IRAEVDIRENYSPGWKYSHWEMKGVPLRIEIGPKDLANNQVRAVRRDNSAKKDIPRALLV
Sbjct: 297 IRAEVDIRENYSPGWKYSHWEMKGVPLRIEIGPKDLANNQVRAVRRDNSAKKDIPRALLV 356

Query: 181 EQVKELLESIQQSLFDAAKEKRDACIQVVNTWEEFTEALGQKKMILAPWCNEEEVEKDVK 240
           EQVKELLESIQQSLFDAAKEKRDACIQVVNTWEEFTEALGQKKMILAPWCNEEEVEKDVK
Sbjct: 357 EQVKELLESIQQSLFDAAKEKRDACIQVVNTWEEFTEALGQKKMILAPWCNEEEVEKDVK 416

Query: 241 TRTKGEMGAAKTLCSPFEQPPLPEGTKCFASGKPAKKWSYWGRSY 286
           TRTKGEMGAAKTLCSPFEQPPLPEGTKCFASGKPAKKWSYWGRSY
Sbjct: 417 TRTKGEMGAAKTLCSPFEQPPLPEGTKCFASGKPAKKWSYWGRSY 461

[Close]

BLAST of Cmc09g0251221 vs. ExPASy TrEMBL
Match: A0A6J1IRP0 (Prolyl-tRNA synthetase OS=Cucurbita maxima OX=3661 GN=LOC111479369 PE=3 SV=1)

HSP 1 Score: 560.1 bits (1442), Expect = 5.4e-156
Identity = 270/285 (94.74%), Postives = 276/285 (96.84%), Query Frame = 0


Query: 1   MEKFAGGLYTTSVEAFIPNTGRGIQGATSHCLGQNFAKMFEINFENEKGEKAMVWQNSWA 60
           MEKFAGGLYTTSVEAFIPNTGRGIQGATSHCLGQNFAKMFEINFENEKGEKAMVWQNSWA
Sbjct: 226 MEKFAGGLYTTSVEAFIPNTGRGIQGATSHCLGQNFAKMFEINFENEKGEKAMVWQNSWA 285

Query: 61  YSTRTIGVMVMVHGDDKGLVMPPKVASVQVIIVPVPYKDADTRGIFDVCSATSDALSKAG 120
           YSTRTIGVMVMVHGDDKGLV+PPKVASVQVIIVPVPYKDADT+GIFD CSAT D LSKAG
Sbjct: 286 YSTRTIGVMVMVHGDDKGLVLPPKVASVQVIIVPVPYKDADTQGIFDACSATLDTLSKAG 345

Query: 121 IRAEVDIRENYSPGWKYSHWEMKGVPLRIEIGPKDLANNQVRAVRRDNSAKKDIPRALLV 180
           IRAEVD R+NYSPGWKYSHWEMKGVPLRIEIGPKDLA+NQVRAVRRDNS KKDIPRA LV
Sbjct: 346 IRAEVDSRDNYSPGWKYSHWEMKGVPLRIEIGPKDLASNQVRAVRRDNSGKKDIPRASLV 405

Query: 181 EQVKELLESIQQSLFDAAKEKRDACIQVVNTWEEFTEALGQKKMILAPWCNEEEVEKDVK 240
           EQVKELLESIQQSLFDAAKEKRD CIQV+NTWEEFTEAL QKKMILAPWC+EEEVEKDVK
Sbjct: 406 EQVKELLESIQQSLFDAAKEKRDVCIQVINTWEEFTEALSQKKMILAPWCDEEEVEKDVK 465

Query: 241 TRTKGEMGAAKTLCSPFEQPPLPEGTKCFASGKPAKKWSYWGRSY 286
           TRTKGEMGAAKTLCSPFEQP LPEGTKCFASGKPAKKWSYWGRSY
Sbjct: 466 TRTKGEMGAAKTLCSPFEQPALPEGTKCFASGKPAKKWSYWGRSY 510

[Close]

BLAST of Cmc09g0251221 vs. ExPASy TrEMBL
Match: A0A6J1GUJ5 (Prolyl-tRNA synthetase OS=Cucurbita moschata OX=3662 GN=LOC111457586 PE=3 SV=1)

HSP 1 Score: 559.3 bits (1440), Expect = 9.3e-156
Identity = 269/285 (94.39%), Postives = 275/285 (96.49%), Query Frame = 0


Query: 1   MEKFAGGLYTTSVEAFIPNTGRGIQGATSHCLGQNFAKMFEINFENEKGEKAMVWQNSWA 60
           MEKFAGGLYTTSVEAFIPNTGRGIQGATSHCLGQNFAKMFEINFENEKGEKAMVWQNSWA
Sbjct: 226 MEKFAGGLYTTSVEAFIPNTGRGIQGATSHCLGQNFAKMFEINFENEKGEKAMVWQNSWA 285

Query: 61  YSTRTIGVMVMVHGDDKGLVMPPKVASVQVIIVPVPYKDADTRGIFDVCSATSDALSKAG 120
           YSTRTIGVMVMVHGDDKGLV+PPKVASVQVIIVPVPYKDADT+GIFD CS T D LS+AG
Sbjct: 286 YSTRTIGVMVMVHGDDKGLVLPPKVASVQVIIVPVPYKDADTQGIFDACSTTLDTLSEAG 345

Query: 121 IRAEVDIRENYSPGWKYSHWEMKGVPLRIEIGPKDLANNQVRAVRRDNSAKKDIPRALLV 180
           IRAEVD R+NYSPGWKYSHWEMKGVPLRIEIGPKDLANNQVRAVRRDNS KKDIPRA LV
Sbjct: 346 IRAEVDSRDNYSPGWKYSHWEMKGVPLRIEIGPKDLANNQVRAVRRDNSGKKDIPRAALV 405

Query: 181 EQVKELLESIQQSLFDAAKEKRDACIQVVNTWEEFTEALGQKKMILAPWCNEEEVEKDVK 240
           EQVKELLESIQQSLFDAAKEKRD CIQV+NTWEEFTEAL QKKMILAPWC+EEEVEKDVK
Sbjct: 406 EQVKELLESIQQSLFDAAKEKRDVCIQVINTWEEFTEALSQKKMILAPWCDEEEVEKDVK 465

Query: 241 TRTKGEMGAAKTLCSPFEQPPLPEGTKCFASGKPAKKWSYWGRSY 286
           TRTKGEMGAAKTLCSPFEQP LPEGTKCFASGKPAKKWSYWGRSY
Sbjct: 466 TRTKGEMGAAKTLCSPFEQPALPEGTKCFASGKPAKKWSYWGRSY 510

[Close]

BLAST of Cmc09g0251221 vs. TAIR 10
Match: AT3G62120.1 (Class II aaRS and biotin synthetases superfamily protein )

HSP 1 Score: 493.8 bits (1270), Expect = 9.2e-140
Identity = 226/284 (79.58%), Postives = 258/284 (90.85%), Query Frame = 0


Query: 2   EKFAGGLYTTSVEAFIPNTGRGIQGATSHCLGQNFAKMFEINFENEKGEKAMVWQNSWAY 61
           EKFAGGLYTTSVEAFIPNTGRG+QGATSHCLGQNFAKMFEINFENEK E  MVWQNSWAY
Sbjct: 247 EKFAGGLYTTSVEAFIPNTGRGVQGATSHCLGQNFAKMFEINFENEKAETEMVWQNSWAY 306

Query: 62  STRTIGVMVMVHGDDKGLVMPPKVASVQVIIVPVPYKDADTRGIFDVCSATSDALSKAGI 121
           STRTIGVM+M HGDDKGLV+PPKVASVQV+++PVPYKDA+T+GI+D C+AT+ AL +AGI
Sbjct: 307 STRTIGVMIMTHGDDKGLVLPPKVASVQVVVIPVPYKDANTQGIYDACTATASALCEAGI 366

Query: 122 RAEVDIRENYSPGWKYSHWEMKGVPLRIEIGPKDLANNQVRAVRRDNSAKKDIPRALLVE 181
           RAE D+R+NYSPGWKYS WEMKGVPLRIEIGP+DL N+QVR VRRDN  K+DIPR  LVE
Sbjct: 367 RAEEDLRDNYSPGWKYSDWEMKGVPLRIEIGPRDLENDQVRTVRRDNGVKEDIPRGSLVE 426

Query: 182 QVKELLESIQQSLFDAAKEKRDACIQVVNTWEEFTEALGQKKMILAPWCNEEEVEKDVKT 241
            VKELLE IQQ++++ AK+KR+AC+Q V TW+EF +AL +KK+ILAPWC+EEEVE+DVK 
Sbjct: 427 HVKELLEKIQQNMYEVAKQKREACVQEVKTWDEFIKALNEKKLILAPWCDEEEVERDVKA 486

Query: 242 RTKGEMGAAKTLCSPFEQPPLPEGTKCFASGKPAKKWSYWGRSY 286
           RTKGE GAAKTLCSPF+QP LPEGT CFASGKPAKKW+YWGRSY
Sbjct: 487 RTKGETGAAKTLCSPFDQPELPEGTLCFASGKPAKKWTYWGRSY 530

[Close]

BLAST of Cmc09g0251221 vs. TAIR 10
Match: AT3G62120.2 (Class II aaRS and biotin synthetases superfamily protein )

HSP 1 Score: 493.8 bits (1270), Expect = 9.2e-140
Identity = 226/284 (79.58%), Postives = 258/284 (90.85%), Query Frame = 0


Query: 2   EKFAGGLYTTSVEAFIPNTGRGIQGATSHCLGQNFAKMFEINFENEKGEKAMVWQNSWAY 61
           EKFAGGLYTTSVEAFIPNTGRG+QGATSHCLGQNFAKMFEINFENEK E  MVWQNSWAY
Sbjct: 247 EKFAGGLYTTSVEAFIPNTGRGVQGATSHCLGQNFAKMFEINFENEKAETEMVWQNSWAY 306

Query: 62  STRTIGVMVMVHGDDKGLVMPPKVASVQVIIVPVPYKDADTRGIFDVCSATSDALSKAGI 121
           STRTIGVM+M HGDDKGLV+PPKVASVQV+++PVPYKDA+T+GI+D C+AT+ AL +AGI
Sbjct: 307 STRTIGVMIMTHGDDKGLVLPPKVASVQVVVIPVPYKDANTQGIYDACTATASALCEAGI 366

Query: 122 RAEVDIRENYSPGWKYSHWEMKGVPLRIEIGPKDLANNQVRAVRRDNSAKKDIPRALLVE 181
           RAE D+R+NYSPGWKYS WEMKGVPLRIEIGP+DL N+QVR VRRDN  K+DIPR  LVE
Sbjct: 367 RAEEDLRDNYSPGWKYSDWEMKGVPLRIEIGPRDLENDQVRTVRRDNGVKEDIPRGSLVE 426

Query: 182 QVKELLESIQQSLFDAAKEKRDACIQVVNTWEEFTEALGQKKMILAPWCNEEEVEKDVKT 241
            VKELLE IQQ++++ AK+KR+AC+Q V TW+EF +AL +KK+ILAPWC+EEEVE+DVK 
Sbjct: 427 HVKELLEKIQQNMYEVAKQKREACVQEVKTWDEFIKALNEKKLILAPWCDEEEVERDVKA 486

Query: 242 RTKGEMGAAKTLCSPFEQPPLPEGTKCFASGKPAKKWSYWGRSY 286
           RTKGE GAAKTLCSPF+QP LPEGT CFASGKPAKKW+YWGRSY
Sbjct: 487 RTKGETGAAKTLCSPFDQPELPEGTLCFASGKPAKKWTYWGRSY 530

[Close]

BLAST of Cmc09g0251221 vs. TAIR 10
Match: AT5G10880.1 (tRNA synthetase-related / tRNA ligase-related )

HSP 1 Score: 286.2 bits (731), Expect = 2.9e-77
Identity = 142/221 (64.25%), Postives = 165/221 (74.66%), Query Frame = 0


Query: 71  MVHGDDKGLVMPPKVASVQVIIVPVPYKD-ADTRGIFDVCSATSDALSKAGIRAEVDIRE 130
           M HGDDKGLV PPKVA VQV+++ VP K  AD + + D C A    L  AGIRAE DIR+
Sbjct: 89  MTHGDDKGLVFPPKVAPVQVVVIHVPIKGAADYQELCDACEAVESTLLGAGIRAEADIRD 148

Query: 131 NYSPGWKYSHWEMKGVPLRIEIGPKDLANNQVRAVRRDNSAKKDIPRALLVEQVKELLES 190
           NYS GWKY+  E+ GVPLRIE GP+DLAN+QVR V RDN AK D+ R  L+EQVK+LLE 
Sbjct: 149 NYSCGWKYADQELTGVPLRIETGPRDLANDQVRIVTRDNGAKMDVKRGDLIEQVKDLLEK 208

Query: 191 IQQSLFDAAKEKRDACIQVVNTWEEFTEALGQKKMILAPWCNEEEVEKDVKTRTKGEM-- 250
           IQ +L+D AK K + C Q V TW+EF EAL QKK+ILAPWC++ EVEKDVK RT+G+   
Sbjct: 209 IQSNLYDVAKRKVEECTQKVETWDEFVEALSQKKLILAPWCDKVEVEKDVKRRTRGDETG 268

Query: 251 ---GAAKTLCSPFEQPPLPEGTKCFASGKPAKKWSYWGRSY 286
              G AKTLC+P EQP L E T CFASGKPAKKWSYWGRSY
Sbjct: 269 GGGGGAKTLCTPLEQPELGEETLCFASGKPAKKWSYWGRSY 309

[Close]

BLAST of Cmc09g0251221 vs. TAIR 10
Match: AT5G52520.1 (Class II aaRS and biotin synthetases superfamily protein )

HSP 1 Score: 226.1 bits (575), Expect = 3.6e-59
Identity = 121/292 (41.44%), Postives = 175/292 (59.93%), Query Frame = 0


Query: 1   MEKFAGGLYTTSVEAFIPNTGRGIQGATSHCLGQNFAKMFEINFENEKGEKAMVWQNSWA 60
           +E FAG   T ++EA + +  + +Q  TSH LGQNF++ F   F +E GE+  VWQ SWA
Sbjct: 261 LETFAGADITYTIEAMMGDR-KALQAGTSHNLGQNFSRAFGTQFADENGERQHVWQTSWA 320

Query: 61  YSTRTIGVMVMVHGDDKGLVMPPKVASVQVIIVPVPYKDADTRGIFDVCSATSDALSKAG 120
            STR +G ++M HGDD GL++PPK+A +QV+IVP+  KD +  G+    S+  +AL  AG
Sbjct: 321 VSTRFVGGIIMTHGDDTGLMLPPKIAPIQVVIVPIWKKDTEKTGVLSAASSVKEALQTAG 380

Query: 121 IRAEVDIRENYSPGWKYSHWEMKGVPLRIEIGPKDLANNQVRAVRRDNSAKK------DI 180
           +R ++D  +  +PGWK++ WEMKG+PLRIEIGP+D+++N V   RRD   K        +
Sbjct: 381 VRVKLDDTDQRTPGWKFNFWEMKGIPLRIEIGPRDVSSNSVVVSRRDVPGKAGKVFGISM 440

Query: 181 PRALLVEQVKELLESIQQSLFDAAKEKRDACIQVVNTWEEFTEALGQKKMILAPWCNEEE 240
             + LV  VKE L+ IQ SL + A   RD+ I  VN++ E  +A+   K    PW   + 
Sbjct: 441 EPSTLVAYVKEKLDEIQTSLLEKALSFRDSNIVDVNSYAELKDAISSGKWARGPWSASDA 500

Query: 241 VEKDVKTRTKGEMGAAKTLCSPFEQPPLPEGTK-CFASGKPAKKWSYWGRSY 286
            E+ VK  T      A   C PFEQ    +GTK C  +G PA++ + + +SY
Sbjct: 501 DEQRVKEET-----GATIRCFPFEQ---TQGTKTCLMTGNPAEEVAIFAKSY 543

The following BLAST results are available for this feature:

BLAST of Cmc09g0251221 vs. NCBI nr


Match Name	E-value	Identity	Description
KAA0050272.1	1.1e-163	100.00	proline--tRNA ligase [Cucumis melo var. makuwa] >TYJ98135.1 proline--tRNA ligase...	[more]
XP_008466343.1	3.3e-163	99.65	PREDICTED: proline--tRNA ligase, cytoplasmic isoform X4 [Cucumis melo]	[more]
XP_008466310.1	3.3e-163	99.65	PREDICTED: proline--tRNA ligase, cytoplasmic isoform X1 [Cucumis melo] >XP_00846...	[more]
XP_022979761.1	1.1e-155	94.74	proline--tRNA ligase, cytoplasmic-like [Cucurbita maxima]	[more]
XP_038907039.1	1.1e-155	94.74	proline--tRNA ligase, cytoplasmic-like isoform X2 [Benincasa hispida]	[more]

BLAST of Cmc09g0251221 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9M1R2	1.3e-138	79.58	Proline--tRNA ligase, cytoplasmic OS=Arabidopsis thaliana OX=3702 GN=At3g62120 P...	[more]
Q8I5R7	4.1e-76	48.14	Proline--tRNA ligase OS=Plasmodium falciparum (isolate 3D7) OX=36329 GN=proRS PE...	[more]
O60155	7.0e-76	49.20	Putative proline--tRNA ligase C19C7.06 OS=Schizosaccharomyces pombe (strain 972 ...	[more]
P28668	5.2e-71	49.17	Bifunctional glutamate/proline--tRNA ligase OS=Drosophila melanogaster OX=7227 G...	[more]
P38708	1.5e-70	46.05	Putative proline--tRNA ligase YHR020W OS=Saccharomyces cerevisiae (strain ATCC 2...	[more]

BLAST of Cmc09g0251221 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7U355	5.4e-164	100.00	Prolyl-tRNA synthetase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1...	[more]
A0A1S4DS87	1.6e-163	99.65	Prolyl-tRNA synthetase OS=Cucumis melo OX=3656 GN=LOC103503756 PE=3 SV=1	[more]
A0A1S3CR78	1.6e-163	99.65	Prolyl-tRNA synthetase OS=Cucumis melo OX=3656 GN=LOC103503756 PE=3 SV=1	[more]
A0A6J1IRP0	5.4e-156	94.74	Prolyl-tRNA synthetase OS=Cucurbita maxima OX=3661 GN=LOC111479369 PE=3 SV=1	[more]
A0A6J1GUJ5	9.3e-156	94.39	Prolyl-tRNA synthetase OS=Cucurbita moschata OX=3662 GN=LOC111457586 PE=3 SV=1	[more]

BLAST of Cmc09g0251221 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G62120.1	9.2e-140	79.58	Class II aaRS and biotin synthetases superfamily protein	[more]
AT3G62120.2	9.2e-140	79.58	Class II aaRS and biotin synthetases superfamily protein	[more]
AT5G10880.1	2.9e-77	64.25	tRNA synthetase-related / tRNA ligase-related	[more]
AT5G52520.1	3.6e-59	41.44	Class II aaRS and biotin synthetases superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Melon (Charmono) v1.1
Date Performed: 2022-10-13


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR016061	Proline-tRNA ligase, class II, C-terminal	SMART	SM00946	ProRS_C_1_2	coord: 212..285 e-value: 1.3E-28 score: 111.0
IPR016061	Proline-tRNA ligase, class II, C-terminal	PFAM	PF09180	ProRS-C_1	coord: 212..285 e-value: 5.2E-27 score: 94.0
None	No IPR available	GENE3D	3.30.930.10	Bira Bifunctional Protein; Domain 2	coord: 1..80 e-value: 1.9E-29 score: 104.8
None	No IPR available	PANTHER	PTHR43382:SF4	PROLINE--TRNA LIGASE, CYTOPLASMIC-LIKE	coord: 1..285
None	No IPR available	CDD	cd00862	ProRS_anticodon_zinc	coord: 78..285 e-value: 1.90696E-94 score: 275.33
None	No IPR available	SUPERFAMILY	55681	Class II aaRS and biotin synthetases	coord: 2..95
None	No IPR available	SUPERFAMILY	52954	Class II aaRS ABD-related	coord: 77..203
IPR004154	Anticodon-binding	PFAM	PF03129	HGTP_anticodon	coord: 89..185 e-value: 1.6E-19 score: 69.8
IPR036621	Anticodon-binding domain superfamily	GENE3D	3.40.50.800		coord: 83..203 e-value: 7.8E-39 score: 134.2
IPR017449	Prolyl-tRNA synthetase, class II	GENE3D	3.30.110.30		coord: 206..285 e-value: 4.2E-29 score: 102.6
IPR017449	Prolyl-tRNA synthetase, class II	SUPERFAMILY	64586	C-terminal domain of ProRS	coord: 207..285
IPR004499	Proline-tRNA ligase, class IIa, archaeal-type	PANTHER	PTHR43382	PROLYL-TRNA SYNTHETASE	coord: 1..285

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cmc09g0251221.1	Cmc09g0251221.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006433	prolyl-tRNA aminoacylation
cellular_component	GO:0017101	aminoacyl-tRNA synthetase multienzyme complex
cellular_component	GO:0005737	cytoplasm
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0004827	proline-tRNA ligase activity
molecular_function	GO:0000166	nucleotide binding