Cmc09g0249731 (gene) Melon (Charmono) v1.1

Overview
NameCmc09g0249731
Typegene
OrganismCucumis melo L. var. cantalupensis cv. Charmono (Melon (Charmono) v1.1)
DescriptionReverse transcriptase Ty1/copia-type domain-containing protein
LocationCMiso1.1chr09: 15688803 .. 15689063 (-)
RNA-Seq ExpressionCmc09g0249731
SyntenyCmc09g0249731
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGATGATAAATACAATGCTCTTATCAAGGCTCAAACATGGTCCTTAGTACTACAGACTTCATCTCAAAATGTCATTGGTAGCAAGTGGGTATTTCGACCAAAACAACATCCTGATGGGTCTATTCATCGCTATAAAGCCCGTTTAGTGGCCAAGGGGTTTCACCAAAATCCTACAGTGGACTTCTTTGAAACTTTCAGTCCTGTAATCATGGCGTTTACAATTCGTGTTATTTTGAGTGTTGCAATCTCTAAAGGATGA

mRNA sequence

ATGGATGATAAATACAATGCTCTTATCAAGGCTCAAACATGGTCCTTAGTACTACAGACTTCATCTCAAAATGTCATTGGTAGCAAGTGGGTATTTCGACCAAAACAACATCCTGATGGGTCTATTCATCGCTATAAAGCCCGTTTAGTGGCCAAGGGGTTTCACCAAAATCCTACAGTGGACTTCTTTGAAACTTTCAGTCCTGTAATCATGGCGTTTACAATTCGTGTTATTTTGAGTGTTGCAATCTCTAAAGGATGA

Coding sequence (CDS)

ATGGATGATAAATACAATGCTCTTATCAAGGCTCAAACATGGTCCTTAGTACTACAGACTTCATCTCAAAATGTCATTGGTAGCAAGTGGGTATTTCGACCAAAACAACATCCTGATGGGTCTATTCATCGCTATAAAGCCCGTTTAGTGGCCAAGGGGTTTCACCAAAATCCTACAGTGGACTTCTTTGAAACTTTCAGTCCTGTAATCATGGCGTTTACAATTCGTGTTATTTTGAGTGTTGCAATCTCTAAAGGATGA

Protein sequence

MDDKYNALIKAQTWSLVLQTSSQNVIGSKWVFRPKQHPDGSIHRYKARLVAKGFHQNPTVDFFETFSPVIMAFTIRVILSVAISKG
Homology
BLAST of Cmc09g0249731 vs. NCBI nr
Match: KAA0066133.1 (putative mitochondrial protein [Cucumis melo var. makuwa] >TYK15176.1 putative mitochondrial protein [Cucumis melo var. makuwa])

HSP 1 Score: 166.8 bits (421), Expect = 8.3e-38
Identity = 80/86 (93.02%), Postives = 83/86 (96.51%), Query Frame = 0

Query: 1   MDDKYNALIKAQTWSLVLQTSSQNVIGSKWVFRPKQHPDGSIHRYKARLVAKGFHQNPTV 60
           MDDKYNAL+KAQTWSLVL TSSQN+IGSKWVFRPKQHPDGSIHRYKARLVAKGFHQNP V
Sbjct: 102 MDDKYNALVKAQTWSLVLPTSSQNLIGSKWVFRPKQHPDGSIHRYKARLVAKGFHQNPRV 161

Query: 61  DFFETFSPVIMAFTIRVILSVAISKG 87
           DFFETFSP+IMA TIRVILSVAISKG
Sbjct: 162 DFFETFSPIIMASTIRVILSVAISKG 187

BLAST of Cmc09g0249731 vs. NCBI nr
Match: XP_016902198.1 (PREDICTED: uncharacterized mitochondrial protein AtMg00810-like isoform X2 [Cucumis melo] >XP_016902199.1 PREDICTED: uncharacterized mitochondrial protein AtMg00810-like isoform X2 [Cucumis melo] >XP_016902200.1 PREDICTED: uncharacterized mitochondrial protein AtMg00810-like isoform X2 [Cucumis melo] >XP_016902201.1 PREDICTED: uncharacterized mitochondrial protein AtMg00810-like isoform X2 [Cucumis melo] >XP_016902202.1 PREDICTED: uncharacterized mitochondrial protein AtMg00810-like isoform X2 [Cucumis melo])

HSP 1 Score: 131.3 bits (329), Expect = 3.9e-27
Identity = 62/86 (72.09%), Postives = 74/86 (86.05%), Query Frame = 0

Query: 1   MDDKYNALIKAQTWSLVLQTSSQNVIGSKWVFRPKQHPDGSIHRYKARLVAKGFHQNPTV 60
           MD++Y+AL+KA TWSLV  + SQNV+GSKWVFR K+H DGSI RYKAR+VAKGFHQ+P V
Sbjct: 20  MDEEYSALLKANTWSLVPPSPSQNVVGSKWVFRLKRHTDGSILRYKARMVAKGFHQHPGV 79

Query: 61  DFFETFSPVIMAFTIRVILSVAISKG 87
           DFFETFSPVI A TIRV+LS+A+  G
Sbjct: 80  DFFETFSPVIKASTIRVVLSIAVPNG 105

BLAST of Cmc09g0249731 vs. NCBI nr
Match: KAA0051295.1 (putative mitochondrial protein [Cucumis melo var. makuwa])

HSP 1 Score: 119.0 bits (297), Expect = 2.0e-23
Identity = 57/86 (66.28%), Postives = 71/86 (82.56%), Query Frame = 0

Query: 1   MDDKYNALIKAQTWSLVLQTSSQNVIGSKWVFRPKQHPDGSIHRYKARLVAKGFHQNPTV 60
           MD++Y+AL+KAQTW LV  +SSQ V+G KWVFR K++ DGSI  YK RLVAKGFHQ+  V
Sbjct: 24  MDEEYSALLKAQTWHLVPPSSSQKVVGKKWVFRLKRNTDGSIRWYKTRLVAKGFHQHLGV 83

Query: 61  DFFETFSPVIMAFTIRVILSVAISKG 87
           DFFETFSPV+ A TIRVIL++A++ G
Sbjct: 84  DFFETFSPVVKASTIRVILNLAVTNG 109

BLAST of Cmc09g0249731 vs. NCBI nr
Match: RVX03627.1 (Retrovirus-related Pol polyprotein from transposon RE1 [Vitis vinifera])

HSP 1 Score: 117.1 bits (292), Expect = 7.6e-23
Identity = 51/86 (59.30%), Postives = 71/86 (82.56%), Query Frame = 0

Query: 1  MDDKYNALIKAQTWSLVLQTSSQNVIGSKWVFRPKQHPDGSIHRYKARLVAKGFHQNPTV 60
          M ++Y+AL++  TW LV   SSQNV+G KW+FR K++ DGSI+R+KARLVAKGFHQ P++
Sbjct: 1  MSEEYDALVRNGTWELVSFNSSQNVVGCKWIFRTKRNSDGSINRFKARLVAKGFHQRPSI 60

Query: 61 DFFETFSPVIMAFTIRVILSVAISKG 87
          D+++TFSPV+   TIR++LSVA+S G
Sbjct: 61 DYYDTFSPVVKPTTIRLVLSVAVSNG 86

BLAST of Cmc09g0249731 vs. NCBI nr
Match: BBH05982.1 (transposable element gene [Prunus dulcis] >BBH08482.1 transposable element gene [Prunus dulcis])

HSP 1 Score: 116.7 bits (291), Expect = 9.9e-23
Identity = 56/86 (65.12%), Postives = 66/86 (76.74%), Query Frame = 0

Query: 1   MDDKYNALIKAQTWSLVLQTSSQNVIGSKWVFRPKQHPDGSIHRYKARLVAKGFHQNPTV 60
           M  ++NALI   TW LV   S+QN+IG KWVFR K+HPDG+I RYKARLVAKGFHQ P V
Sbjct: 108 MSTEFNALISNGTWELVPSDSTQNLIGCKWVFRIKRHPDGTIDRYKARLVAKGFHQRPGV 167

Query: 61  DFFETFSPVIMAFTIRVILSVAISKG 87
           DF ETFSPV+   TIRV+L +A+S G
Sbjct: 168 DFSETFSPVVKPTTIRVVLHLALSNG 193

BLAST of Cmc09g0249731 vs. ExPASy Swiss-Prot
Match: P92520 (Uncharacterized mitochondrial protein AtMg00820 OS=Arabidopsis thaliana OX=3702 GN=AtMg00820 PE=4 SV=1)

HSP 1 Score: 90.9 bits (224), Expect = 7.6e-18
Identity = 43/82 (52.44%), Postives = 58/82 (70.73%), Query Frame = 0

Query: 1   MDDKYNALIKAQTWSLVLQTSSQNVIGSKWVFRPKQHPDGSIHRYKARLVAKGFHQNPTV 60
           M ++ +AL + +TW LV    +QN++G KWVF+ K H DG++ R KARLVAKGFHQ   +
Sbjct: 44  MQEELDALSRNKTWILVPPPVNQNILGCKWVFKTKLHSDGTLDRLKARLVAKGFHQEEGI 103

Query: 61  DFFETFSPVIMAFTIRVILSVA 83
            F ET+SPV+   TIR IL+VA
Sbjct: 104 YFVETYSPVVRTATIRTILNVA 125

BLAST of Cmc09g0249731 vs. ExPASy Swiss-Prot
Match: Q94HW2 (Retrovirus-related Pol polyprotein from transposon RE1 OS=Arabidopsis thaliana OX=3702 GN=RE1 PE=2 SV=1)

HSP 1 Score: 86.7 bits (213), Expect = 1.4e-16
Identity = 41/86 (47.67%), Postives = 59/86 (68.60%), Query Frame = 0

Query: 1    MDDKYNALIKAQTWSLVLQTSSQ-NVIGSKWVFRPKQHPDGSIHRYKARLVAKGFHQNPT 60
            M  + NA I   TW LV    S   ++G +W+F  K + DGS++RYKARLVAKG++Q P 
Sbjct: 972  MGSEINAQIGNHTWDLVPPPPSHVTIVGCRWIFTKKYNSDGSLNRYKARLVAKGYNQRPG 1031

Query: 61   VDFFETFSPVIMAFTIRVILSVAISK 86
            +D+ ETFSPVI + +IR++L VA+ +
Sbjct: 1032 LDYAETFSPVIKSTSIRIVLGVAVDR 1057

BLAST of Cmc09g0249731 vs. ExPASy Swiss-Prot
Match: Q9ZT94 (Retrovirus-related Pol polyprotein from transposon RE2 OS=Arabidopsis thaliana OX=3702 GN=RE2 PE=4 SV=1)

HSP 1 Score: 85.1 bits (209), Expect = 4.2e-16
Identity = 41/86 (47.67%), Postives = 59/86 (68.60%), Query Frame = 0

Query: 1    MDDKYNALIKAQTWSLV-LQTSSQNVIGSKWVFRPKQHPDGSIHRYKARLVAKGFHQNPT 60
            M  + NA I   TW LV     S  ++G +W+F  K + DGS++RYKARLVAKG++Q P 
Sbjct: 955  MGSEINAQIGNHTWDLVPPPPPSVTIVGCRWIFTKKFNSDGSLNRYKARLVAKGYNQRPG 1014

Query: 61   VDFFETFSPVIMAFTIRVILSVAISK 86
            +D+ ETFSPVI + +IR++L VA+ +
Sbjct: 1015 LDYAETFSPVIKSTSIRIVLGVAVDR 1040

BLAST of Cmc09g0249731 vs. ExPASy Swiss-Prot
Match: P10978 (Retrovirus-related Pol polyprotein from transposon TNT 1-94 OS=Nicotiana tabacum OX=4097 PE=2 SV=1)

HSP 1 Score: 68.2 bits (165), Expect = 5.3e-11
Identity = 37/84 (44.05%), Postives = 50/84 (59.52%), Query Frame = 0

Query: 1   MDDKYNALIKAQTWSLVLQTSSQNVIGSKWVFRPKQHPDGSIHRYKARLVAKGFHQNPTV 60
           M ++  +L K  T+ LV     +  +  KWVF+ K+  D  + RYKARLV KGF Q   +
Sbjct: 830 MQEEMESLQKNGTYKLVELPKGKRPLKCKWVFKLKKDGDCKLVRYKARLVVKGFEQKKGI 889

Query: 61  DFFETFSPVIMAFTIRVILSVAIS 85
           DF E FSPV+   +IR ILS+A S
Sbjct: 890 DFDEIFSPVVKMTSIRTILSLAAS 913

BLAST of Cmc09g0249731 vs. ExPASy Swiss-Prot
Match: P04146 (Copia protein OS=Drosophila melanogaster OX=7227 GN=GIP PE=1 SV=3)

HSP 1 Score: 65.5 bits (158), Expect = 3.4e-10
Identity = 31/71 (43.66%), Postives = 47/71 (66.20%), Query Frame = 0

Query: 13  TWSLVLQTSSQNVIGSKWVFRPKQHPDGSIHRYKARLVAKGFHQNPTVDFFETFSPVIMA 72
           TW++  +  ++N++ S+WVF  K +  G+  RYKARLVA+GF Q   +D+ ETF+PV   
Sbjct: 922 TWTITKRPENKNIVDSRWVFSVKYNELGNPIRYKARLVARGFTQKYQIDYEETFAPVARI 981

Query: 73  FTIRVILSVAI 84
            + R ILS+ I
Sbjct: 982 SSFRFILSLVI 992

BLAST of Cmc09g0249731 vs. ExPASy TrEMBL
Match: A0A5A7VD76 (Putative mitochondrial protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold790G00440 PE=4 SV=1)

HSP 1 Score: 166.8 bits (421), Expect = 4.0e-38
Identity = 80/86 (93.02%), Postives = 83/86 (96.51%), Query Frame = 0

Query: 1   MDDKYNALIKAQTWSLVLQTSSQNVIGSKWVFRPKQHPDGSIHRYKARLVAKGFHQNPTV 60
           MDDKYNAL+KAQTWSLVL TSSQN+IGSKWVFRPKQHPDGSIHRYKARLVAKGFHQNP V
Sbjct: 102 MDDKYNALVKAQTWSLVLPTSSQNLIGSKWVFRPKQHPDGSIHRYKARLVAKGFHQNPRV 161

Query: 61  DFFETFSPVIMAFTIRVILSVAISKG 87
           DFFETFSP+IMA TIRVILSVAISKG
Sbjct: 162 DFFETFSPIIMASTIRVILSVAISKG 187

BLAST of Cmc09g0249731 vs. ExPASy TrEMBL
Match: A0A1S4E1U4 (uncharacterized mitochondrial protein AtMg00810-like isoform X2 OS=Cucumis melo OX=3656 GN=LOC107991581 PE=4 SV=1)

HSP 1 Score: 131.3 bits (329), Expect = 1.9e-27
Identity = 62/86 (72.09%), Postives = 74/86 (86.05%), Query Frame = 0

Query: 1   MDDKYNALIKAQTWSLVLQTSSQNVIGSKWVFRPKQHPDGSIHRYKARLVAKGFHQNPTV 60
           MD++Y+AL+KA TWSLV  + SQNV+GSKWVFR K+H DGSI RYKAR+VAKGFHQ+P V
Sbjct: 20  MDEEYSALLKANTWSLVPPSPSQNVVGSKWVFRLKRHTDGSILRYKARMVAKGFHQHPGV 79

Query: 61  DFFETFSPVIMAFTIRVILSVAISKG 87
           DFFETFSPVI A TIRV+LS+A+  G
Sbjct: 80  DFFETFSPVIKASTIRVVLSIAVPNG 105

BLAST of Cmc09g0249731 vs. ExPASy TrEMBL
Match: A0A5A7UCD7 (Putative mitochondrial protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold55G00020 PE=4 SV=1)

HSP 1 Score: 119.0 bits (297), Expect = 9.7e-24
Identity = 57/86 (66.28%), Postives = 71/86 (82.56%), Query Frame = 0

Query: 1   MDDKYNALIKAQTWSLVLQTSSQNVIGSKWVFRPKQHPDGSIHRYKARLVAKGFHQNPTV 60
           MD++Y+AL+KAQTW LV  +SSQ V+G KWVFR K++ DGSI  YK RLVAKGFHQ+  V
Sbjct: 24  MDEEYSALLKAQTWHLVPPSSSQKVVGKKWVFRLKRNTDGSIRWYKTRLVAKGFHQHLGV 83

Query: 61  DFFETFSPVIMAFTIRVILSVAISKG 87
           DFFETFSPV+ A TIRVIL++A++ G
Sbjct: 84  DFFETFSPVVKASTIRVILNLAVTNG 109

BLAST of Cmc09g0249731 vs. ExPASy TrEMBL
Match: A0A438J3U4 (Retrovirus-related Pol polyprotein from transposon RE1 OS=Vitis vinifera OX=29760 GN=RE1_2777 PE=4 SV=1)

HSP 1 Score: 117.1 bits (292), Expect = 3.7e-23
Identity = 51/86 (59.30%), Postives = 71/86 (82.56%), Query Frame = 0

Query: 1  MDDKYNALIKAQTWSLVLQTSSQNVIGSKWVFRPKQHPDGSIHRYKARLVAKGFHQNPTV 60
          M ++Y+AL++  TW LV   SSQNV+G KW+FR K++ DGSI+R+KARLVAKGFHQ P++
Sbjct: 1  MSEEYDALVRNGTWELVSFNSSQNVVGCKWIFRTKRNSDGSINRFKARLVAKGFHQRPSI 60

Query: 61 DFFETFSPVIMAFTIRVILSVAISKG 87
          D+++TFSPV+   TIR++LSVA+S G
Sbjct: 61 DYYDTFSPVVKPTTIRLVLSVAVSNG 86

BLAST of Cmc09g0249731 vs. ExPASy TrEMBL
Match: A0A4Y1RVT7 (Transposable element protein OS=Prunus dulcis OX=3755 GN=Prudu_017525 PE=4 SV=1)

HSP 1 Score: 116.7 bits (291), Expect = 4.8e-23
Identity = 56/86 (65.12%), Postives = 66/86 (76.74%), Query Frame = 0

Query: 1   MDDKYNALIKAQTWSLVLQTSSQNVIGSKWVFRPKQHPDGSIHRYKARLVAKGFHQNPTV 60
           M  ++NALI   TW LV   S+QN+IG KWVFR K+HPDG+I RYKARLVAKGFHQ P V
Sbjct: 108 MSTEFNALISNGTWELVPSDSTQNLIGCKWVFRIKRHPDGTIDRYKARLVAKGFHQRPGV 167

Query: 61  DFFETFSPVIMAFTIRVILSVAISKG 87
           DF ETFSPV+   TIRV+L +A+S G
Sbjct: 168 DFSETFSPVVKPTTIRVVLHLALSNG 193

BLAST of Cmc09g0249731 vs. TAIR 10
Match: ATMG00820.1 (Reverse transcriptase (RNA-dependent DNA polymerase) )

HSP 1 Score: 90.9 bits (224), Expect = 5.4e-19
Identity = 43/82 (52.44%), Postives = 58/82 (70.73%), Query Frame = 0

Query: 1   MDDKYNALIKAQTWSLVLQTSSQNVIGSKWVFRPKQHPDGSIHRYKARLVAKGFHQNPTV 60
           M ++ +AL + +TW LV    +QN++G KWVF+ K H DG++ R KARLVAKGFHQ   +
Sbjct: 44  MQEELDALSRNKTWILVPPPVNQNILGCKWVFKTKLHSDGTLDRLKARLVAKGFHQEEGI 103

Query: 61  DFFETFSPVIMAFTIRVILSVA 83
            F ET+SPV+   TIR IL+VA
Sbjct: 104 YFVETYSPVVRTATIRTILNVA 125

BLAST of Cmc09g0249731 vs. TAIR 10
Match: AT4G23160.1 (cysteine-rich RLK (RECEPTOR-like protein kinase) 8 )

HSP 1 Score: 80.1 bits (196), Expect = 9.6e-16
Identity = 36/82 (43.90%), Postives = 54/82 (65.85%), Query Frame = 0

Query: 1   MDDKYNALIKAQTWSLVLQTSSQNVIGSKWVFRPKQHPDGSIHRYKARLVAKGFHQNPTV 60
           MDD+  A+    TW +     ++  IG KWV++ K + DG+I RYKARLVAKG+ Q   +
Sbjct: 102 MDDEIGAMETTHTWEICTLPPNKKPIGCKWVYKIKYNSDGTIERYKARLVAKGYTQQEGI 161

Query: 61  DFFETFSPVIMAFTIRVILSVA 83
           DF ETFSPV    ++++IL+++
Sbjct: 162 DFIETFSPVCKLTSVKLILAIS 183

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAA0066133.18.3e-3893.02putative mitochondrial protein [Cucumis melo var. makuwa] >TYK15176.1 putative m... [more]
XP_016902198.13.9e-2772.09PREDICTED: uncharacterized mitochondrial protein AtMg00810-like isoform X2 [Cucu... [more]
KAA0051295.12.0e-2366.28putative mitochondrial protein [Cucumis melo var. makuwa][more]
RVX03627.17.6e-2359.30Retrovirus-related Pol polyprotein from transposon RE1 [Vitis vinifera][more]
BBH05982.19.9e-2365.12transposable element gene [Prunus dulcis] >BBH08482.1 transposable element gene ... [more]
Match NameE-valueIdentityDescription
P925207.6e-1852.44Uncharacterized mitochondrial protein AtMg00820 OS=Arabidopsis thaliana OX=3702 ... [more]
Q94HW21.4e-1647.67Retrovirus-related Pol polyprotein from transposon RE1 OS=Arabidopsis thaliana O... [more]
Q9ZT944.2e-1647.67Retrovirus-related Pol polyprotein from transposon RE2 OS=Arabidopsis thaliana O... [more]
P109785.3e-1144.05Retrovirus-related Pol polyprotein from transposon TNT 1-94 OS=Nicotiana tabacum... [more]
P041463.4e-1043.66Copia protein OS=Drosophila melanogaster OX=7227 GN=GIP PE=1 SV=3[more]
Match NameE-valueIdentityDescription
A0A5A7VD764.0e-3893.02Putative mitochondrial protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_s... [more]
A0A1S4E1U41.9e-2772.09uncharacterized mitochondrial protein AtMg00810-like isoform X2 OS=Cucumis melo ... [more]
A0A5A7UCD79.7e-2466.28Putative mitochondrial protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_s... [more]
A0A438J3U43.7e-2359.30Retrovirus-related Pol polyprotein from transposon RE1 OS=Vitis vinifera OX=2976... [more]
A0A4Y1RVT74.8e-2365.12Transposable element protein OS=Prunus dulcis OX=3755 GN=Prudu_017525 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
ATMG00820.15.4e-1952.44Reverse transcriptase (RNA-dependent DNA polymerase) [more]
AT4G23160.19.6e-1643.90cysteine-rich RLK (RECEPTOR-like protein kinase) 8 [more]
InterPro
Analysis Name: InterPro Annotations of Melon (Charmono) v1.1
Date Performed: 2022-10-13
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR013103Reverse transcriptase, RNA-dependent DNA polymerasePFAMPF07727RVT_2coord: 12..84
e-value: 1.8E-16
score: 60.5

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cmc09g0249731.1Cmc09g0249731.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006974 cellular response to DNA damage stimulus
biological_process GO:0051276 chromosome organization
biological_process GO:0015074 DNA integration
molecular_function GO:0050660 flavin adenine dinucleotide binding
molecular_function GO:0004386 helicase activity
molecular_function GO:0016787 hydrolase activity
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0016491 oxidoreductase activity