Cmc09g0241741 (gene) Melon (Charmono) v1.1

Overview
NameCmc09g0241741
Typegene
OrganismCucumis melo L. var. cantalupensis cv. Charmono (Melon (Charmono) v1.1)
Descriptionsirohydrochlorin ferrochelatase, chloroplastic
LocationCMiso1.1chr09: 3880820 .. 3883383 (-)
RNA-Seq ExpressionCmc09g0241741
SyntenyCmc09g0241741
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TTTAGTAAAACCCACCACTTTCATTTTAGGCCAATGCAATTGAATTTACCAACGACGTCGTTTTTGATTATCAGGTTCAAGTGCTCCCTCTTCCCTTGGCGAAGGATCAGAACCCATGTCTTCGTTATCCGTCACTCCTCAAATTTCACCGAAGAGGTTTCAAAATCTCCTAAACCCTCATTTTCTTTTCAATTTTTTCTTGATTTTTTTCCTCTCTAATGGCCGTCCATTTTCCCATTGAAGTCCTTCACCAGTGGAAACTAGAAGCCCATACACAATCTGCGCGTCTCAGAGATCTCCAATTCCTATCGGACGCCTCTCCAAATTCAGAAATTTCACCTCGCGGGTCCGCATGAGTATTGATAATGGAAGCTCTAATCGGATTGGTGCCGGAGATGCTGTGATTATCGTCGACCACGGCTCCCGTCGCCGGGAATCTAATCGCATGCTGAGTAAGTTAGGGTTTTAGAATTTGGAATTTTGGGTGGGTGAAGTTTTGAAGTTATTCGTTGGATAATTCTCTTGGGGTGATGAACTTTTTTGGAAATTTTCAGATGAGTTTGTTGCTATGTTTAGGGATAAAACTGGTTATCCCATTGTGGAGCCTGCTCACATGGTATGTTCTTTTCAACTGTTTCTTGTGCACAAAGCTACTACTTTCATTCATGAAGCAACATTATTTGCTTTTGTAGTTGGTTTTTGGAAATCAATGTTACATTTTAGTTAGGGCTGCTTGGGTTGTAATGAAATGAGTGTGTAATTTAATGGGTCCAAAACCCGTGTTTGGATTTAGCCTTTCTGCCCGATTTGTAATCCCAAACTCATGAGTTTCTTTCTGCAGTTTTTAATCTAACCCTCTTTTCTACGATTTCCGTGCTTGATGATTCTATTTCGCATCTCAATGCTTCTCAATTCTTATTGCATTCTAGCTCTCCAAACATTTAGTTAAAAGTATTTTGGTCCCTGTAATTATAGATTTAGTTCATTTTAGTTCATGTAAGTAAATGTATATTTTGGTCCCATAAGTGATATTTTGAAAGTATTGGGATCGAAATGAACATTTTGAAAATATAACGACCAACTGTAAGGACCAAACTAACATTTTAAACCTTTTGTTTGGTTCAACAATGGTGGTGCTAACCACTTCTTTTTGGTATATGTGTAGACCAATTGAACTATGTGTTAAATTATTAACATTTGATCGATCAATTAAGACTGAAAGCAAACCATGATGAAGTAAACTCAACATCACGATTGGATATTTTGATTTTCGAAAAAATGTACAGTTTTGTGGATCTTTTTTCTACACTCTGAAGTACAGCATCTCTAGACTTCAAATTAACATAAGACCCAATGAATAGAAAAGCAAAATAAATACTGATACCATTCAAAATGAATACTGATTTGGAACAATTCTATGTCATCGTACTGTCAGTAGTTTCTCTGTCTTTATTCCTGTCTGTCTCCTTTTGTTGCATTCAACACATGATAGAGTAATAGTCACAGCCATTTCCAATGTACTTAATGGTCTCATTACATTTTTCTGAAACAACATAGGAGCTGGCCGAACCATCGATAAGAGACTCATTCACATCATGTGTTGAACAAGGGGCCAAACGTATCATCGTCAGCCCGTTCTTTCTTTTCCCTGGTCGACATTGGCAACAGGTATGTAACTCACTGTAGGACACTTGATTTGATGAAATGAACTGGTGGATTGATGATAGCAATGAGTGAAACTTTGCAGGATATTCCTTCCTTAACTGCTGAAGCAGCAAAGGATCACCCAGGAATATCGTATATAATCACCGCTCCTCTCGGTCTTCACGAAAAACTTGTGGTAGGTATTCCTAACCTATATTTTTAATACGAATAGGAATATTAATAACAATTTCCCCAAGGAAAAACAATTTAATTGTTAATCTAAATAAATTTTATAAATTTCGTGACTTCCATAAATATCTGTCGATATGTCCGTTTTTTCGATGTGTCCGTAAAATTGAAATCTCTATATATCGATATTGACATTGTCAATTCCCAGATGGAAGAATGAATGTCATAAATGATAAAAGAAAAGGACTATCTTTTAGTATTAAACTATGAGGTGGTTCTGTTGCAGGATGTTGTGAACGATAGAATAAATCACTGCTTAAGCCATGCTGCTGGAGTGGCAGACGAATGTGAAGTTTGTGCTGGCACGGGCAAATGTCGATTTAACTAGTGTATGTAAGTAGAGACTTGGGATGAAATCGCTCGGTTTTTCGACGACTTTCATTTGAAGGGCCCATAAAAATGGTATCGATGGCTTGGCAGTTGAAGGCTTGAGAGTCTATTTATCTTGTTTCGTATCAATACTTTGTAAAATTTAACCTTTTTGGTTGTTTTTGTATATTATATCTTCACACATGACTACAGTCGACAACAATATGCACAATAATATTAGTAACCAAAAATGATTTTGAGAACAGTTTGAGATTAGTGATAGAATATCAGAGCTTTTTCATGAACCTACCTATCACTAAAAACTTGCTTTACCCAATTCCAAACTCCATAAAACTTCTCCC

mRNA sequence

TTTAGTAAAACCCACCACTTTCATTTTAGGCCAATGCAATTGAATTTACCAACGACGTCGTTTTTGATTATCAGGTTCAAGTGCTCCCTCTTCCCTTGGCGAAGGATCAGAACCCATGTCTTCGTTATCCGTCACTCCTCAAATTTCACCGAAGAGTCCTTCACCAGTGGAAACTAGAAGCCCATACACAATCTGCGCGTCTCAGAGATCTCCAATTCCTATCGGACGCCTCTCCAAATTCAGAAATTTCACCTCGCGGGTCCGCATGAGTATTGATAATGGAAGCTCTAATCGGATTGGTGCCGGAGATGCTGTGATTATCGTCGACCACGGCTCCCGTCGCCGGGAATCTAATCGCATGCTGAATGAGTTTGTTGCTATGTTTAGGGATAAAACTGGTTATCCCATTGTGGAGCCTGCTCACATGCTGGCCGAACCATCGATAAGAGACTCATTCACATCATGTGTTGAACAAGGGGCCAAACGTATCATCGTCAGCCCGTTCTTTCTTTTCCCTGGTCGACATTGGCAACAGGATATTCCTTCCTTAACTGCTGAAGCAGCAAAGGATCACCCAGGAATATCGTATATAATCACCGCTCCTCTCGGTCTTCACGAAAAACTTGTGGATGTTGTGAACGATAGAATAAATCACTGCTTAAGCCATGCTGCTGGAGTGGCAGACGAATGTGAAGTTTGTGCTGGCACGGGCAAATGTCGATTTAACTAGTGTATGTAAGTAGAGACTTGGGATGAAATCGCTCGGTTTTTCGACGACTTTCATTTGAAGGGCCCATAAAAATGGTATCGATGGCTTGGCAGTTGAAGGCTTGAGAGTCTATTTATCTTGTTTCGTATCAATACTTTGTAAAATTTAACCTTTTTGGTTGTTTTTGTATATTATATCTTCACACATGACTACAGTCGACAACAATATGCACAATAATATTAGTAACCAAAAATGATTTTGAGAACAGTTTGAGATTAGTGATAGAATATCAGAGCTTTTTCATGAACCTACCTATCACTAAAAACTTGCTTTACCCAATTCCAAACTCCATAAAACTTCTCCC

Coding sequence (CDS)

ATGTCTTCGTTATCCGTCACTCCTCAAATTTCACCGAAGAGTCCTTCACCAGTGGAAACTAGAAGCCCATACACAATCTGCGCGTCTCAGAGATCTCCAATTCCTATCGGACGCCTCTCCAAATTCAGAAATTTCACCTCGCGGGTCCGCATGAGTATTGATAATGGAAGCTCTAATCGGATTGGTGCCGGAGATGCTGTGATTATCGTCGACCACGGCTCCCGTCGCCGGGAATCTAATCGCATGCTGAATGAGTTTGTTGCTATGTTTAGGGATAAAACTGGTTATCCCATTGTGGAGCCTGCTCACATGCTGGCCGAACCATCGATAAGAGACTCATTCACATCATGTGTTGAACAAGGGGCCAAACGTATCATCGTCAGCCCGTTCTTTCTTTTCCCTGGTCGACATTGGCAACAGGATATTCCTTCCTTAACTGCTGAAGCAGCAAAGGATCACCCAGGAATATCGTATATAATCACCGCTCCTCTCGGTCTTCACGAAAAACTTGTGGATGTTGTGAACGATAGAATAAATCACTGCTTAAGCCATGCTGCTGGAGTGGCAGACGAATGTGAAGTTTGTGCTGGCACGGGCAAATGTCGATTTAACTAG

Protein sequence

MSSLSVTPQISPKSPSPVETRSPYTICASQRSPIPIGRLSKFRNFTSRVRMSIDNGSSNRIGAGDAVIIVDHGSRRRESNRMLNEFVAMFRDKTGYPIVEPAHMLAEPSIRDSFTSCVEQGAKRIIVSPFFLFPGRHWQQDIPSLTAEAAKDHPGISYIITAPLGLHEKLVDVVNDRINHCLSHAAGVADECEVCAGTGKCRFN
Homology
BLAST of Cmc09g0241741 vs. NCBI nr
Match: XP_008458790.1 (PREDICTED: sirohydrochlorin ferrochelatase, chloroplastic [Cucumis melo])

HSP 1 Score: 411.4 bits (1056), Expect = 4.6e-111
Identity = 204/205 (99.51%), Postives = 204/205 (99.51%), Query Frame = 0

Query: 1   MSSLSVTPQISPKSPSPVETRSPYTICASQRSPIPIGRLSKFRNFTSRVRMSIDNGSSNR 60
           MSSLSVTPQISPKSPSPVETRSPYTICASQRSPIPIGRLSKFRNFTSRVRMSIDNGSSNR
Sbjct: 1   MSSLSVTPQISPKSPSPVETRSPYTICASQRSPIPIGRLSKFRNFTSRVRMSIDNGSSNR 60

Query: 61  IGAGDAVIIVDHGSRRRESNRMLNEFVAMFRDKTGYPIVEPAHM-LAEPSIRDSFTSCVE 120
           IGAGDAVIIVDHGSRRRESNRMLNEFVAMFRDKTGYPIVEPAHM LAEPSIRDSFTSCVE
Sbjct: 61  IGAGDAVIIVDHGSRRRESNRMLNEFVAMFRDKTGYPIVEPAHMELAEPSIRDSFTSCVE 120

Query: 121 QGAKRIIVSPFFLFPGRHWQQDIPSLTAEAAKDHPGISYIITAPLGLHEKLVDVVNDRIN 180
           QGAKRIIVSPFFLFPGRHWQQDIPSLTAEAAKDHPGISYIITAPLGLHEKLVDVVNDRIN
Sbjct: 121 QGAKRIIVSPFFLFPGRHWQQDIPSLTAEAAKDHPGISYIITAPLGLHEKLVDVVNDRIN 180

Query: 181 HCLSHAAGVADECEVCAGTGKCRFN 205
           HCLSHAAGVADECEVCAGTGKCRFN
Sbjct: 181 HCLSHAAGVADECEVCAGTGKCRFN 205

BLAST of Cmc09g0241741 vs. NCBI nr
Match: KAA0037556.1 (sirohydrochlorin ferrochelatase [Cucumis melo var. makuwa] >TYK03116.1 sirohydrochlorin ferrochelatase [Cucumis melo var. makuwa])

HSP 1 Score: 406.4 bits (1043), Expect = 1.5e-109
Identity = 202/205 (98.54%), Postives = 202/205 (98.54%), Query Frame = 0

Query: 1   MSSLSVTPQISPKSPSPVETRSPYTICASQRSPIPIGRLSKFRNFTSRVRMSIDNGSSNR 60
           MSSLSVTPQISPKSPSPVETRSPYTICA QRSPIPIGRLSKFRNFTSRVRMSIDNGSSNR
Sbjct: 1   MSSLSVTPQISPKSPSPVETRSPYTICAFQRSPIPIGRLSKFRNFTSRVRMSIDNGSSNR 60

Query: 61  IGAGDAVIIVDHGSRRRESNRMLNEFVAMFRDKTGYPIVEPAHM-LAEPSIRDSFTSCVE 120
           IGAGDAVIIVDHGSRRRESN MLNEFVAMFRDKTGYPIVEPAHM LAEPSIRDSFTSCVE
Sbjct: 61  IGAGDAVIIVDHGSRRRESNLMLNEFVAMFRDKTGYPIVEPAHMELAEPSIRDSFTSCVE 120

Query: 121 QGAKRIIVSPFFLFPGRHWQQDIPSLTAEAAKDHPGISYIITAPLGLHEKLVDVVNDRIN 180
           QGAKRIIVSPFFLFPGRHWQQDIPSLTAEAAKDHPGISYIITAPLGLHEKLVDVVNDRIN
Sbjct: 121 QGAKRIIVSPFFLFPGRHWQQDIPSLTAEAAKDHPGISYIITAPLGLHEKLVDVVNDRIN 180

Query: 181 HCLSHAAGVADECEVCAGTGKCRFN 205
           HCLSHAAGVADECEVCAGTGKCRFN
Sbjct: 181 HCLSHAAGVADECEVCAGTGKCRFN 205

BLAST of Cmc09g0241741 vs. NCBI nr
Match: XP_031742215.1 (sirohydrochlorin ferrochelatase, chloroplastic [Cucumis sativus] >KGN51370.1 hypothetical protein Csa_008200 [Cucumis sativus])

HSP 1 Score: 379.8 bits (974), Expect = 1.5e-101
Identity = 187/205 (91.22%), Postives = 196/205 (95.61%), Query Frame = 0

Query: 1   MSSLSVTPQISPKSPSPVETRSPYTICASQRSPIPIGRLSKFRNFTSRVRMSIDNGSSNR 60
           MSS S+TPQISPKS SPVETR  ++ICASQRS IP+GRLSKFRNFTSR RMSIDNGSSNR
Sbjct: 1   MSSFSITPQISPKSSSPVETRRLFSICASQRSSIPVGRLSKFRNFTSRFRMSIDNGSSNR 60

Query: 61  IGAGDAVIIVDHGSRRRESNRMLNEFVAMFRDKTGYPIVEPAHM-LAEPSIRDSFTSCVE 120
           IGAGDAVIIVDHGSRRRESN MLNEFVAMF+DKTGYPIVEPAHM LAEPSI+DSFT CVE
Sbjct: 61  IGAGDAVIIVDHGSRRRESNLMLNEFVAMFKDKTGYPIVEPAHMELAEPSIKDSFTLCVE 120

Query: 121 QGAKRIIVSPFFLFPGRHWQQDIPSLTAEAAKDHPGISYIITAPLGLHEKLVDVVNDRIN 180
           QGAKRIIVSPFFLFPGRHWQQDIPSLTAEAAKDHPGISYIITAPLGLHE+LVDVVNDRIN
Sbjct: 121 QGAKRIIVSPFFLFPGRHWQQDIPSLTAEAAKDHPGISYIITAPLGLHEQLVDVVNDRIN 180

Query: 181 HCLSHAAGVADECEVCAGTGKCRFN 205
           +CLSHAAGV+DECEVCAGTGKCRFN
Sbjct: 181 YCLSHAAGVSDECEVCAGTGKCRFN 205

BLAST of Cmc09g0241741 vs. NCBI nr
Match: XP_038890885.1 (sirohydrochlorin ferrochelatase, chloroplastic isoform X1 [Benincasa hispida])

HSP 1 Score: 362.8 bits (930), Expect = 1.9e-96
Identity = 187/210 (89.05%), Postives = 193/210 (91.90%), Query Frame = 0

Query: 1   MSSLS-VTPQISPKSPSPVETRSPYTICASQRSPIPIGRLSKFRNFTSRVRMSIDNGSSN 60
           M+SLS VTPQIS KS S VETR  YTICASQ+SPI I RLS+FRNFTSR RMS+DNGSSN
Sbjct: 37  MTSLSIVTPQISLKSSSAVETRRKYTICASQKSPILIRRLSRFRNFTSRFRMSVDNGSSN 96

Query: 61  ----RIGAGDAVIIVDHGSRRRESNRMLNEFVAMFRDKTGYPIVEPAHM-LAEPSIRDSF 120
               RIGAGDAVIIVDHGSRRRESN MLNEFVAMFRDKTGYPIVEPAHM LAEPSIRDSF
Sbjct: 97  RSGDRIGAGDAVIIVDHGSRRRESNLMLNEFVAMFRDKTGYPIVEPAHMELAEPSIRDSF 156

Query: 121 TSCVEQGAKRIIVSPFFLFPGRHWQQDIPSLTAEAAKDHPGISYIITAPLGLHEKLVDVV 180
             CVEQGAKRIIVSPFFLFPGRHWQQDIPSLTAEAAKDHPGISYIITAPLGLHEKLVDVV
Sbjct: 157 ALCVEQGAKRIIVSPFFLFPGRHWQQDIPSLTAEAAKDHPGISYIITAPLGLHEKLVDVV 216

Query: 181 NDRINHCLSHAAGVADECEVCAGTGKCRFN 205
           NDRI+HCLSHAAGVA+ECEVCAGTGKCRFN
Sbjct: 217 NDRISHCLSHAAGVAEECEVCAGTGKCRFN 246

BLAST of Cmc09g0241741 vs. NCBI nr
Match: XP_022993758.1 (sirohydrochlorin ferrochelatase, chloroplastic-like [Cucurbita maxima])

HSP 1 Score: 354.8 bits (909), Expect = 5.1e-94
Identity = 177/207 (85.51%), Postives = 188/207 (90.82%), Query Frame = 0

Query: 1   MSSLSVTPQISPKSPSPVETRSPYTICASQRSP--IPIGRLSKFRNFTSRVRMSIDNGSS 60
           M+SLS+ PQI+ KSPS  ETR  YTICASQ+S   I IGR S  RNF+SR RMS+DNG S
Sbjct: 1   MTSLSIPPQITVKSPSAGETRKTYTICASQKSSILIGIGRSSTLRNFSSRFRMSVDNGGS 60

Query: 61  NRIGAGDAVIIVDHGSRRRESNRMLNEFVAMFRDKTGYPIVEPAHM-LAEPSIRDSFTSC 120
           NRIGAGDAVIIVDHGSRRRESN MLNEFVAMFRDKTGYPIVEPAHM LAEPSIRDSF SC
Sbjct: 61  NRIGAGDAVIIVDHGSRRRESNLMLNEFVAMFRDKTGYPIVEPAHMELAEPSIRDSFASC 120

Query: 121 VEQGAKRIIVSPFFLFPGRHWQQDIPSLTAEAAKDHPGISYIITAPLGLHEKLVDVVNDR 180
           VEQGAKRI+VSPFFLFPGRHWQQDIPSLTAEAAKDHPGISYIITAPLGLHE+LVDVVNDR
Sbjct: 121 VEQGAKRIVVSPFFLFPGRHWQQDIPSLTAEAAKDHPGISYIITAPLGLHEQLVDVVNDR 180

Query: 181 INHCLSHAAGVADECEVCAGTGKCRFN 205
           I+HCLSHAAGV +ECEVCAGTGKC+FN
Sbjct: 181 ISHCLSHAAGVVEECEVCAGTGKCKFN 207

BLAST of Cmc09g0241741 vs. ExPASy Swiss-Prot
Match: Q84JH7 (Sirohydrochlorin ferrochelatase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=SIRB PE=1 SV=1)

HSP 1 Score: 233.4 bits (594), Expect = 2.3e-60
Identity = 109/145 (75.17%), Postives = 123/145 (84.83%), Query Frame = 0

Query: 59  NRIGAGDAVIIVDHGSRRRESNRMLNEFVAMFRDKTGYPIVEPAHM-LAEPSIRDSFTSC 118
           N IG  D +IIVDHGSRRRESN ML EFV MF++KTGYPIVEPAHM LAEPSI+D+F+ C
Sbjct: 76  NGIGDADGIIIVDHGSRRRESNLMLEEFVKMFKEKTGYPIVEPAHMELAEPSIKDAFSLC 135

Query: 119 VEQGAKRIIVSPFFLFPGRHWQQDIPSLTAEAAKDHPGISYIITAPLGLHEKLVDVVNDR 178
           V+QGAKR++VSPFFLFPGRHW  DIPSLTA+AAK+  GISY+ITAPLG H  L+DVVNDR
Sbjct: 136 VQQGAKRVVVSPFFLFPGRHWHTDIPSLTADAAKEFSGISYLITAPLGPHNLLLDVVNDR 195

Query: 179 INHCLSHAAGVADECEVCAGTGKCR 203
           I HCLSH  G ADEC VCAGT KC+
Sbjct: 196 IQHCLSHVEGDADECLVCAGTNKCK 220

BLAST of Cmc09g0241741 vs. ExPASy Swiss-Prot
Match: P61817 (Sirohydrochlorin ferrochelatase OS=Bacillus megaterium OX=1404 GN=sirB PE=3 SV=1)

HSP 1 Score: 80.9 bits (198), Expect = 1.9e-14
Identity = 40/116 (34.48%), Postives = 61/116 (52.59%), Query Frame = 0

Query: 65  DAVIIVDHGSRRRESNRMLNEFVAMFRDKTGYPIVEPAHM-LAEPSIRDSFTSCVEQGAK 124
           DAV+ V HGSR +E       F+   +     PI E   + LA P+I   F +C+EQGA 
Sbjct: 13  DAVLYVCHGSRVKEGADQAVAFIERCKKNLDVPIQEVCFLELASPTIEQGFEACIEQGAT 72

Query: 125 RIIVSPFFLFPGRHWQQDIPSLTAEAAKDHPGISYIITAPLGLHEKLVDVVNDRIN 180
           RI + P  L    H + DIP    +  + +P +  +   P G+ E++VD++ +RIN
Sbjct: 73  RIAIVPLLLLTAAHAKHDIPEEIQKVYERYPQVEVLYGEPFGVDERIVDILVERIN 128

BLAST of Cmc09g0241741 vs. ExPASy Swiss-Prot
Match: P61819 (Sirohydrochlorin cobaltochelatase OS=Methanococcus maripaludis (strain S2 / LL) OX=267377 GN=cbiX PE=3 SV=1)

HSP 1 Score: 72.4 bits (176), Expect = 6.7e-12
Identity = 40/141 (28.37%), Postives = 66/141 (46.81%), Query Frame = 0

Query: 65  DAVIIVDHGSRRRESNRMLNEFVAMFRDKTGYPIVEPAHM-LAEPSIRDSFTSCVEQGAK 124
           +A+++V HGSR   S  ++ E     + +  Y IVE   M   EP+I  +    ++ GAK
Sbjct: 2   EALVLVGHGSRLPHSKNVVMEVAEKIKARNIYDIVEVGMMEFNEPTIPQTIKKVIDAGAK 61

Query: 125 RIIVSPFFLFPGRHWQQDIPSLTA------EAAKDH-------------------PGISY 180
           ++IV+P FL PG H ++DIP +        E    H                    G+  
Sbjct: 62  KVIVTPVFLAPGNHTERDIPKILGIYEGDDEGGHHHHHDHEHHHHHHDTTAVEIPEGVEL 121

BLAST of Cmc09g0241741 vs. ExPASy Swiss-Prot
Match: Q58380 (Sirohydrochlorin cobaltochelatase OS=Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) OX=243232 GN=cbiX PE=1 SV=1)

HSP 1 Score: 70.5 bits (171), Expect = 2.5e-11
Identity = 42/138 (30.43%), Postives = 65/138 (47.10%), Query Frame = 0

Query: 65  DAVIIVDHGSRRRESNRMLNEFVAMFRDKTGYPIVEPAHM-LAEPSIRDSFTSCVEQGAK 124
           +A+++V HGSR   S  +L +     +++  +PIVE   M  +EP+I  +    +EQGAK
Sbjct: 2   EALVLVGHGSRLPYSKELLVKLAEKVKERNLFPIVEIGLMEFSEPTIPQAVKKAIEQGAK 61

Query: 125 RIIVSPFFLFPGRHWQQDIPSLTAEAAKDH------------------------PGISYI 178
           RIIV P FL  G H  +DIP L      +H                          +  I
Sbjct: 62  RIIVVPVFLAHGIHTTRDIPRLLGLIEDNHEHHHEHSHHHHHHHHHEHEKLEIPEDVEII 121

BLAST of Cmc09g0241741 vs. ExPASy Swiss-Prot
Match: A6UWT5 (Sirohydrochlorin cobaltochelatase OS=Methanococcus aeolicus (strain ATCC BAA-1280 / DSM 17508 / OCM 812 / Nankai-3) OX=419665 GN=cbiX PE=3 SV=1)

HSP 1 Score: 66.2 bits (160), Expect = 4.8e-10
Identity = 39/138 (28.26%), Postives = 65/138 (47.10%), Query Frame = 0

Query: 65  DAVIIVDHGSRRRESNRMLNEFVAMFRDKTGYPIVEPAHM-LAEPSIRDSFTSCVEQGAK 124
           +A++++ HGSR   S  ++ +     ++K  Y IVE   M   EP+I  +    + +GAK
Sbjct: 2   EALVLLGHGSRLPYSKEIVGKVAEKIKEKNIYDIVEIGMMEFNEPTIPQTINKVIAEGAK 61

Query: 125 RIIVSPFFLFPGRHWQQDIPSLTA--EAAKDH----------------------PGISYI 178
           +II+ P FL  G H ++DIP +    E  + H                       G+  I
Sbjct: 62  KIIIVPVFLAHGNHTKRDIPQILGLIECEEHHHEGEGGHHHHHHHHHGEKIEVPEGVEII 121

BLAST of Cmc09g0241741 vs. ExPASy TrEMBL
Match: A0A1S3C8S9 (sirohydrochlorin ferrochelatase, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103498093 PE=4 SV=1)

HSP 1 Score: 411.4 bits (1056), Expect = 2.2e-111
Identity = 204/205 (99.51%), Postives = 204/205 (99.51%), Query Frame = 0

Query: 1   MSSLSVTPQISPKSPSPVETRSPYTICASQRSPIPIGRLSKFRNFTSRVRMSIDNGSSNR 60
           MSSLSVTPQISPKSPSPVETRSPYTICASQRSPIPIGRLSKFRNFTSRVRMSIDNGSSNR
Sbjct: 1   MSSLSVTPQISPKSPSPVETRSPYTICASQRSPIPIGRLSKFRNFTSRVRMSIDNGSSNR 60

Query: 61  IGAGDAVIIVDHGSRRRESNRMLNEFVAMFRDKTGYPIVEPAHM-LAEPSIRDSFTSCVE 120
           IGAGDAVIIVDHGSRRRESNRMLNEFVAMFRDKTGYPIVEPAHM LAEPSIRDSFTSCVE
Sbjct: 61  IGAGDAVIIVDHGSRRRESNRMLNEFVAMFRDKTGYPIVEPAHMELAEPSIRDSFTSCVE 120

Query: 121 QGAKRIIVSPFFLFPGRHWQQDIPSLTAEAAKDHPGISYIITAPLGLHEKLVDVVNDRIN 180
           QGAKRIIVSPFFLFPGRHWQQDIPSLTAEAAKDHPGISYIITAPLGLHEKLVDVVNDRIN
Sbjct: 121 QGAKRIIVSPFFLFPGRHWQQDIPSLTAEAAKDHPGISYIITAPLGLHEKLVDVVNDRIN 180

Query: 181 HCLSHAAGVADECEVCAGTGKCRFN 205
           HCLSHAAGVADECEVCAGTGKCRFN
Sbjct: 181 HCLSHAAGVADECEVCAGTGKCRFN 205

BLAST of Cmc09g0241741 vs. ExPASy TrEMBL
Match: A0A5D3BY55 (Sirohydrochlorin ferrochelatase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold374G00520 PE=4 SV=1)

HSP 1 Score: 406.4 bits (1043), Expect = 7.2e-110
Identity = 202/205 (98.54%), Postives = 202/205 (98.54%), Query Frame = 0

Query: 1   MSSLSVTPQISPKSPSPVETRSPYTICASQRSPIPIGRLSKFRNFTSRVRMSIDNGSSNR 60
           MSSLSVTPQISPKSPSPVETRSPYTICA QRSPIPIGRLSKFRNFTSRVRMSIDNGSSNR
Sbjct: 1   MSSLSVTPQISPKSPSPVETRSPYTICAFQRSPIPIGRLSKFRNFTSRVRMSIDNGSSNR 60

Query: 61  IGAGDAVIIVDHGSRRRESNRMLNEFVAMFRDKTGYPIVEPAHM-LAEPSIRDSFTSCVE 120
           IGAGDAVIIVDHGSRRRESN MLNEFVAMFRDKTGYPIVEPAHM LAEPSIRDSFTSCVE
Sbjct: 61  IGAGDAVIIVDHGSRRRESNLMLNEFVAMFRDKTGYPIVEPAHMELAEPSIRDSFTSCVE 120

Query: 121 QGAKRIIVSPFFLFPGRHWQQDIPSLTAEAAKDHPGISYIITAPLGLHEKLVDVVNDRIN 180
           QGAKRIIVSPFFLFPGRHWQQDIPSLTAEAAKDHPGISYIITAPLGLHEKLVDVVNDRIN
Sbjct: 121 QGAKRIIVSPFFLFPGRHWQQDIPSLTAEAAKDHPGISYIITAPLGLHEKLVDVVNDRIN 180

Query: 181 HCLSHAAGVADECEVCAGTGKCRFN 205
           HCLSHAAGVADECEVCAGTGKCRFN
Sbjct: 181 HCLSHAAGVADECEVCAGTGKCRFN 205

BLAST of Cmc09g0241741 vs. ExPASy TrEMBL
Match: A0A0A0KP17 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G523150 PE=4 SV=1)

HSP 1 Score: 379.8 bits (974), Expect = 7.2e-102
Identity = 187/205 (91.22%), Postives = 196/205 (95.61%), Query Frame = 0

Query: 1   MSSLSVTPQISPKSPSPVETRSPYTICASQRSPIPIGRLSKFRNFTSRVRMSIDNGSSNR 60
           MSS S+TPQISPKS SPVETR  ++ICASQRS IP+GRLSKFRNFTSR RMSIDNGSSNR
Sbjct: 1   MSSFSITPQISPKSSSPVETRRLFSICASQRSSIPVGRLSKFRNFTSRFRMSIDNGSSNR 60

Query: 61  IGAGDAVIIVDHGSRRRESNRMLNEFVAMFRDKTGYPIVEPAHM-LAEPSIRDSFTSCVE 120
           IGAGDAVIIVDHGSRRRESN MLNEFVAMF+DKTGYPIVEPAHM LAEPSI+DSFT CVE
Sbjct: 61  IGAGDAVIIVDHGSRRRESNLMLNEFVAMFKDKTGYPIVEPAHMELAEPSIKDSFTLCVE 120

Query: 121 QGAKRIIVSPFFLFPGRHWQQDIPSLTAEAAKDHPGISYIITAPLGLHEKLVDVVNDRIN 180
           QGAKRIIVSPFFLFPGRHWQQDIPSLTAEAAKDHPGISYIITAPLGLHE+LVDVVNDRIN
Sbjct: 121 QGAKRIIVSPFFLFPGRHWQQDIPSLTAEAAKDHPGISYIITAPLGLHEQLVDVVNDRIN 180

Query: 181 HCLSHAAGVADECEVCAGTGKCRFN 205
           +CLSHAAGV+DECEVCAGTGKCRFN
Sbjct: 181 YCLSHAAGVSDECEVCAGTGKCRFN 205

BLAST of Cmc09g0241741 vs. ExPASy TrEMBL
Match: A0A6J1K384 (sirohydrochlorin ferrochelatase, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111489668 PE=4 SV=1)

HSP 1 Score: 354.8 bits (909), Expect = 2.5e-94
Identity = 177/207 (85.51%), Postives = 188/207 (90.82%), Query Frame = 0

Query: 1   MSSLSVTPQISPKSPSPVETRSPYTICASQRSP--IPIGRLSKFRNFTSRVRMSIDNGSS 60
           M+SLS+ PQI+ KSPS  ETR  YTICASQ+S   I IGR S  RNF+SR RMS+DNG S
Sbjct: 1   MTSLSIPPQITVKSPSAGETRKTYTICASQKSSILIGIGRSSTLRNFSSRFRMSVDNGGS 60

Query: 61  NRIGAGDAVIIVDHGSRRRESNRMLNEFVAMFRDKTGYPIVEPAHM-LAEPSIRDSFTSC 120
           NRIGAGDAVIIVDHGSRRRESN MLNEFVAMFRDKTGYPIVEPAHM LAEPSIRDSF SC
Sbjct: 61  NRIGAGDAVIIVDHGSRRRESNLMLNEFVAMFRDKTGYPIVEPAHMELAEPSIRDSFASC 120

Query: 121 VEQGAKRIIVSPFFLFPGRHWQQDIPSLTAEAAKDHPGISYIITAPLGLHEKLVDVVNDR 180
           VEQGAKRI+VSPFFLFPGRHWQQDIPSLTAEAAKDHPGISYIITAPLGLHE+LVDVVNDR
Sbjct: 121 VEQGAKRIVVSPFFLFPGRHWQQDIPSLTAEAAKDHPGISYIITAPLGLHEQLVDVVNDR 180

Query: 181 INHCLSHAAGVADECEVCAGTGKCRFN 205
           I+HCLSHAAGV +ECEVCAGTGKC+FN
Sbjct: 181 ISHCLSHAAGVVEECEVCAGTGKCKFN 207

BLAST of Cmc09g0241741 vs. ExPASy TrEMBL
Match: A0A6J1FHT5 (sirohydrochlorin ferrochelatase, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111445470 PE=4 SV=1)

HSP 1 Score: 348.2 bits (892), Expect = 2.3e-92
Identity = 173/207 (83.57%), Postives = 187/207 (90.34%), Query Frame = 0

Query: 1   MSSLSVTPQISPKSPSPVETRSPYTICASQRSP--IPIGRLSKFRNFTSRVRMSIDNGSS 60
           M+SLS+  QI+ KSPS  ETR  YTICASQ+S   I +GR S  RNF+SR RMS+DNG +
Sbjct: 1   MTSLSIPSQITVKSPSAGETRKTYTICASQKSSILIGVGRSSTLRNFSSRFRMSVDNGGA 60

Query: 61  NRIGAGDAVIIVDHGSRRRESNRMLNEFVAMFRDKTGYPIVEPAHM-LAEPSIRDSFTSC 120
           +RIGAGDAVIIVDHGSRRRESN MLNEFVAMFRDKTGYPIVEPAHM LAEPSIRDSF SC
Sbjct: 61  DRIGAGDAVIIVDHGSRRRESNLMLNEFVAMFRDKTGYPIVEPAHMELAEPSIRDSFASC 120

Query: 121 VEQGAKRIIVSPFFLFPGRHWQQDIPSLTAEAAKDHPGISYIITAPLGLHEKLVDVVNDR 180
           VEQGAKRI+VSPFFLFPGRHWQQDIPSLTAEAAKDHPGISYIITAPLGLHE+LVDVVNDR
Sbjct: 121 VEQGAKRIVVSPFFLFPGRHWQQDIPSLTAEAAKDHPGISYIITAPLGLHEQLVDVVNDR 180

Query: 181 INHCLSHAAGVADECEVCAGTGKCRFN 205
           I+HCLSHAAGV +ECEVCAGTGKC+FN
Sbjct: 181 ISHCLSHAAGVVEECEVCAGTGKCKFN 207

BLAST of Cmc09g0241741 vs. TAIR 10
Match: AT1G50170.1 (sirohydrochlorin ferrochelatase B )

HSP 1 Score: 233.4 bits (594), Expect = 1.6e-61
Identity = 109/145 (75.17%), Postives = 123/145 (84.83%), Query Frame = 0

Query: 59  NRIGAGDAVIIVDHGSRRRESNRMLNEFVAMFRDKTGYPIVEPAHM-LAEPSIRDSFTSC 118
           N IG  D +IIVDHGSRRRESN ML EFV MF++KTGYPIVEPAHM LAEPSI+D+F+ C
Sbjct: 76  NGIGDADGIIIVDHGSRRRESNLMLEEFVKMFKEKTGYPIVEPAHMELAEPSIKDAFSLC 135

Query: 119 VEQGAKRIIVSPFFLFPGRHWQQDIPSLTAEAAKDHPGISYIITAPLGLHEKLVDVVNDR 178
           V+QGAKR++VSPFFLFPGRHW  DIPSLTA+AAK+  GISY+ITAPLG H  L+DVVNDR
Sbjct: 136 VQQGAKRVVVSPFFLFPGRHWHTDIPSLTADAAKEFSGISYLITAPLGPHNLLLDVVNDR 195

Query: 179 INHCLSHAAGVADECEVCAGTGKCR 203
           I HCLSH  G ADEC VCAGT KC+
Sbjct: 196 IQHCLSHVEGDADECLVCAGTNKCK 220

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_008458790.14.6e-11199.51PREDICTED: sirohydrochlorin ferrochelatase, chloroplastic [Cucumis melo][more]
KAA0037556.11.5e-10998.54sirohydrochlorin ferrochelatase [Cucumis melo var. makuwa] >TYK03116.1 sirohydro... [more]
XP_031742215.11.5e-10191.22sirohydrochlorin ferrochelatase, chloroplastic [Cucumis sativus] >KGN51370.1 hyp... [more]
XP_038890885.11.9e-9689.05sirohydrochlorin ferrochelatase, chloroplastic isoform X1 [Benincasa hispida][more]
XP_022993758.15.1e-9485.51sirohydrochlorin ferrochelatase, chloroplastic-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
Q84JH72.3e-6075.17Sirohydrochlorin ferrochelatase, chloroplastic OS=Arabidopsis thaliana OX=3702 G... [more]
P618171.9e-1434.48Sirohydrochlorin ferrochelatase OS=Bacillus megaterium OX=1404 GN=sirB PE=3 SV=1[more]
P618196.7e-1228.37Sirohydrochlorin cobaltochelatase OS=Methanococcus maripaludis (strain S2 / LL) ... [more]
Q583802.5e-1130.43Sirohydrochlorin cobaltochelatase OS=Methanocaldococcus jannaschii (strain ATCC ... [more]
A6UWT54.8e-1028.26Sirohydrochlorin cobaltochelatase OS=Methanococcus aeolicus (strain ATCC BAA-128... [more]
Match NameE-valueIdentityDescription
A0A1S3C8S92.2e-11199.51sirohydrochlorin ferrochelatase, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103... [more]
A0A5D3BY557.2e-11098.54Sirohydrochlorin ferrochelatase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_... [more]
A0A0A0KP177.2e-10291.22Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G523150 PE=4 SV=1[more]
A0A6J1K3842.5e-9485.51sirohydrochlorin ferrochelatase, chloroplastic-like OS=Cucurbita maxima OX=3661 ... [more]
A0A6J1FHT52.3e-9283.57sirohydrochlorin ferrochelatase, chloroplastic-like OS=Cucurbita moschata OX=366... [more]
Match NameE-valueIdentityDescription
AT1G50170.11.6e-6175.17sirohydrochlorin ferrochelatase B [more]
InterPro
Analysis Name: InterPro Annotations of Melon (Charmono) v1.1
Date Performed: 2022-10-13
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.40.50.1400coord: 63..185
e-value: 3.0E-32
score: 113.5
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..23
NoneNo IPR availablePANTHERPTHR33542SIROHYDROCHLORIN FERROCHELATASE, CHLOROPLASTICcoord: 57..201
NoneNo IPR availablePANTHERPTHR33542:SF3SIROHYDROCHLORIN FERROCHELATASE, CHLOROPLASTICcoord: 57..201
NoneNo IPR availableCDDcd03416CbiX_SirB_Ncoord: 66..165
e-value: 4.18606E-36
score: 120.369
NoneNo IPR availableSUPERFAMILY53800Chelatasecoord: 65..181
IPR002762Sirohydrochlorin cobaltochelatase CbiX-likePFAMPF01903CbiXcoord: 72..175
e-value: 9.7E-28
score: 96.6

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cmc09g0241741.1Cmc09g0241741.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0016829 lyase activity
molecular_function GO:0046872 metal ion binding