Cmc08g0228421 (gene) Melon (Charmono) v1.1

Overview
NameCmc08g0228421
Typegene
OrganismCucumis melo L. var. cantalupensis cv. Charmono (Melon (Charmono) v1.1)
DescriptionProtein FAR1-RELATED SEQUENCE 4
LocationCMiso1.1chr08: 22656059 .. 22656637 (+)
RNA-Seq ExpressionCmc08g0228421
SyntenyCmc08g0228421
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTCTACTGATCGTTCCATTCCTAAAGAGATTGTGCATAAGACTCGTACAAATCTTGGAGTTAATATAAGTTACCAAAAACCTTGGAGGGCAAAAGAACATATGGTAAAGATATTACATGGTGACACAGTTGAATCGTATGCCTTGATTCAAAGATTCTTTGATAAATTGGTTGAATCTAACCCAGGTATGATTAGTTTTGTATATCAAATTTAAATTGATTTGCAGATAGATGTGGATAGACTAATATCACAGTTTATCTAGATAGACTAATATTGACATCTTTCATATTCTTTCTATTCTTTTTTGTTTGTGCTGATAGAAATGTGTTTTTAATTATTATAGGATGATAGTGGTCATTTCAAGTTTTACTTTATGGCTTTTGATGCATCAATTGAGGGGTGGAAATATTGTAGACCTATTATTTCTATTGATGGGACATTTTTGAAGTGTAAGTTTGGTGGCATCCTATTAATAGCCTCATCACAAGATGGTAACAATCAAATCTTCCCTCTTGCTTTTGCCATTGTAGATTCTGAAAATGATGTATCATGGACATGGTTTTTTGAGAAGATATGA

mRNA sequence

ATGTCTACTGATCGTTCCATTCCTAAAGAGATTGTGCATAAGACTCGTACAAATCTTGGAGTTAATATAAGTTACCAAAAACCTTGGAGGGCAAAAGAACATATGGTAAAGATATTACATGGTGACACAGTTGAATCGTATGCCTTGATTCAAAGATTCTTTGATAAATTGGATGATAGTGGTCATTTCAAGTTTTACTTTATGGCTTTTGATGCATCAATTGAGGGGTGGAAATATTGTAGACCTATTATTTCTATTGATGGGACATTTTTGAAGTGTAAGTTTGGTGGCATCCTATTAATAGCCTCATCACAAGATGGTAACAATCAAATCTTCCCTCTTGCTTTTGCCATTGTAGATTCTGAAAATGATGTATCATGGACATGGTTTTTTGAGAAGATATGA

Coding sequence (CDS)

ATGTCTACTGATCGTTCCATTCCTAAAGAGATTGTGCATAAGACTCGTACAAATCTTGGAGTTAATATAAGTTACCAAAAACCTTGGAGGGCAAAAGAACATATGGTAAAGATATTACATGGTGACACAGTTGAATCGTATGCCTTGATTCAAAGATTCTTTGATAAATTGGATGATAGTGGTCATTTCAAGTTTTACTTTATGGCTTTTGATGCATCAATTGAGGGGTGGAAATATTGTAGACCTATTATTTCTATTGATGGGACATTTTTGAAGTGTAAGTTTGGTGGCATCCTATTAATAGCCTCATCACAAGATGGTAACAATCAAATCTTCCCTCTTGCTTTTGCCATTGTAGATTCTGAAAATGATGTATCATGGACATGGTTTTTTGAGAAGATATGA

Protein sequence

MSTDRSIPKEIVHKTRTNLGVNISYQKPWRAKEHMVKILHGDTVESYALIQRFFDKLDDSGHFKFYFMAFDASIEGWKYCRPIISIDGTFLKCKFGGILLIASSQDGNNQIFPLAFAIVDSENDVSWTWFFEKI
Homology
BLAST of Cmc08g0228421 vs. NCBI nr
Match: KAA0036500.1 (protein FAR1-RELATED SEQUENCE 4 [Cucumis melo var. makuwa] >TYJ99981.1 protein FAR1-RELATED SEQUENCE 4 [Cucumis melo var. makuwa])

HSP 1 Score: 244.6 bits (623), Expect = 4.9e-61
Identity = 123/147 (83.67%), Postives = 123/147 (83.67%), Query Frame = 0

Query: 1   MSTDRSIPKEIVHKTRTNLGVNISYQKPWRAKEHMVKILHGDTVESYALIQRFFDKL--- 60
           MSTD SIPKEI HKTRTNLGVNISYQK WR KEHMVKILHGDTVESYALI RFFDKL   
Sbjct: 19  MSTDPSIPKEIEHKTRTNLGVNISYQKAWRLKEHMVKILHGDTVESYALISRFFDKLVKS 78

Query: 61  ----------DDSGHFKFYFMAFDASIEGWKYCRPIISIDGTFLKCKFGGILLIASSQDG 120
                     DDSGHFKF FMAF ASIE WKYC PIISIDGTFLKCKFG ILL ASSQDG
Sbjct: 79  NPGTCTALEMDDSGHFKFCFMAFGASIERWKYCIPIISIDGTFLKCKFGDILLTASSQDG 138

Query: 121 NNQIFPLAFAIVDSENDVSWTWFFEKI 135
           NNQIFPLAFAIVDSENDVSWTWFFEKI
Sbjct: 139 NNQIFPLAFAIVDSENDVSWTWFFEKI 165

BLAST of Cmc08g0228421 vs. NCBI nr
Match: KAA0035384.1 (protein FAR-RED IMPAIRED RESPONSE 1-like [Cucumis melo var. makuwa] >TYK03496.1 protein FAR-RED IMPAIRED RESPONSE 1-like [Cucumis melo var. makuwa])

HSP 1 Score: 240.7 bits (613), Expect = 7.1e-60
Identity = 119/147 (80.95%), Postives = 120/147 (81.63%), Query Frame = 0

Query: 1   MSTDRSIPKEIVHKTRTNLGVNISYQKPWRAKEHMVKILHGDTVESYALIQRFFDKL--- 60
           MSTDRSIPKEIVHK  TNLGVNISYQK WR KEHMVKILH D VESYALI RFFDKL   
Sbjct: 19  MSTDRSIPKEIVHKAHTNLGVNISYQKAWRVKEHMVKILHDDKVESYALIPRFFDKLVES 78

Query: 61  ----------DDSGHFKFYFMAFDASIEGWKYCRPIISIDGTFLKCKFGGILLIASSQDG 120
                     DDSGHFKF FMAF ASIEGWKYCR IIS+DGTFLKCKFGGILL ASSQDG
Sbjct: 79  NPSTCTALEMDDSGHFKFCFMAFGASIEGWKYCRTIISVDGTFLKCKFGGILLAASSQDG 138

Query: 121 NNQIFPLAFAIVDSENDVSWTWFFEKI 135
           NNQIFPLAF IVD END SWTWFFEKI
Sbjct: 139 NNQIFPLAFVIVDFENDASWTWFFEKI 165

BLAST of Cmc08g0228421 vs. NCBI nr
Match: KAA0047748.1 (uncharacterized protein E6C27_scaffold1059G00070 [Cucumis melo var. makuwa] >TYK02382.1 uncharacterized protein E5676_scaffold155G00850 [Cucumis melo var. makuwa])

HSP 1 Score: 240.0 bits (611), Expect = 1.2e-59
Identity = 119/147 (80.95%), Postives = 121/147 (82.31%), Query Frame = 0

Query: 1   MSTDRSIPKEIVHKTRTNLGVNISYQKPWRAKEHMVKILHGDTVESYALIQRFFDKL--- 60
           MSTDRSIPKEIVHK  TNLGVN SYQK WRAKEHMVKILHGDTVE YALI RFFDKL   
Sbjct: 135 MSTDRSIPKEIVHKACTNLGVNTSYQKAWRAKEHMVKILHGDTVELYALIPRFFDKLVES 194

Query: 61  ----------DDSGHFKFYFMAFDASIEGWKYCRPIISIDGTFLKCKFGGILLIASSQDG 120
                     DD+GHFKF FM F ASIEGWKYCRPIIS+D TFLKCKFGGI L ASSQDG
Sbjct: 195 NPGTCTALEMDDNGHFKFCFMDFGASIEGWKYCRPIISVDRTFLKCKFGGIRLTASSQDG 254

Query: 121 NNQIFPLAFAIVDSENDVSWTWFFEKI 135
           NNQIFPLAFAIVDSEND SWTWFFEKI
Sbjct: 255 NNQIFPLAFAIVDSENDASWTWFFEKI 281

BLAST of Cmc08g0228421 vs. NCBI nr
Match: KAA0050206.1 (uncharacterized protein E6C27_scaffold355G00380 [Cucumis melo var. makuwa] >TYJ98206.1 uncharacterized protein E5676_scaffold180G00990 [Cucumis melo var. makuwa])

HSP 1 Score: 236.1 bits (601), Expect = 1.7e-58
Identity = 117/147 (79.59%), Postives = 120/147 (81.63%), Query Frame = 0

Query: 1   MSTDRSIPKEIVHKTRTNLGVNISYQKPWRAKEHMVKILHGDTVESYALIQRFFDKL--- 60
           MSTDRSIPKEIVHK RT LGV ISYQK WR KEH+VKILHGDTVESYALI RFFDKL   
Sbjct: 1   MSTDRSIPKEIVHKARTKLGVKISYQKAWRTKEHIVKILHGDTVESYALILRFFDKLVES 60

Query: 61  ----------DDSGHFKFYFMAFDASIEGWKYCRPIISIDGTFLKCKFGGILLIASSQDG 120
                     DDSGHFKF FMAF AS+EGWKYCR IISIDG FLKCKFGGILL ASSQDG
Sbjct: 61  NPGTCTALEMDDSGHFKFCFMAFGASVEGWKYCRSIISIDGIFLKCKFGGILLTASSQDG 120

Query: 121 NNQIFPLAFAIVDSENDVSWTWFFEKI 135
           N+QIFPLAFAIVDSE D  WTWFFEKI
Sbjct: 121 NDQIFPLAFAIVDSEKDALWTWFFEKI 147

BLAST of Cmc08g0228421 vs. NCBI nr
Match: TYK02872.1 (protein FAR1-RELATED SEQUENCE 4 [Cucumis melo var. makuwa])

HSP 1 Score: 230.3 bits (586), Expect = 9.6e-57
Identity = 113/141 (80.14%), Postives = 118/141 (83.69%), Query Frame = 0

Query: 1   MSTDRSIPKEIVHKTRTNLGVNISYQKPWRAKEHMVKILHGDTVESYALIQRFFDKL--- 60
           MST+RSIP EIVHK RTNLG+NISYQK WRAKEHMVKILHGDTVESYALI RFFDKL   
Sbjct: 19  MSTNRSIPDEIVHKARTNLGINISYQKAWRAKEHMVKILHGDTVESYALIPRFFDKLVES 78

Query: 61  ----------DDSGHFKFYFMAFDASIEGWKYCRPIISIDGTFLKCKFGGILLIASSQDG 120
                     DDSGHFKF FMAF ASIEGWKYC+PII +DG FLKCKFGGILL ASSQDG
Sbjct: 79  NPGTCTALEMDDSGHFKFCFMAFGASIEGWKYCKPIIYVDGKFLKCKFGGILLTASSQDG 138

Query: 121 NNQIFPLAFAIVDSENDVSWT 129
           NNQIFPLAF IVDS+NDVSWT
Sbjct: 139 NNQIFPLAFTIVDSKNDVSWT 159

BLAST of Cmc08g0228421 vs. ExPASy TrEMBL
Match: A0A5D3BMN4 (Protein FAR1-RELATED SEQUENCE 4 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold360G00260 PE=4 SV=1)

HSP 1 Score: 244.6 bits (623), Expect = 2.4e-61
Identity = 123/147 (83.67%), Postives = 123/147 (83.67%), Query Frame = 0

Query: 1   MSTDRSIPKEIVHKTRTNLGVNISYQKPWRAKEHMVKILHGDTVESYALIQRFFDKL--- 60
           MSTD SIPKEI HKTRTNLGVNISYQK WR KEHMVKILHGDTVESYALI RFFDKL   
Sbjct: 19  MSTDPSIPKEIEHKTRTNLGVNISYQKAWRLKEHMVKILHGDTVESYALISRFFDKLVKS 78

Query: 61  ----------DDSGHFKFYFMAFDASIEGWKYCRPIISIDGTFLKCKFGGILLIASSQDG 120
                     DDSGHFKF FMAF ASIE WKYC PIISIDGTFLKCKFG ILL ASSQDG
Sbjct: 79  NPGTCTALEMDDSGHFKFCFMAFGASIERWKYCIPIISIDGTFLKCKFGDILLTASSQDG 138

Query: 121 NNQIFPLAFAIVDSENDVSWTWFFEKI 135
           NNQIFPLAFAIVDSENDVSWTWFFEKI
Sbjct: 139 NNQIFPLAFAIVDSENDVSWTWFFEKI 165

BLAST of Cmc08g0228421 vs. ExPASy TrEMBL
Match: A0A5D3BZ76 (Protein FAR-RED IMPAIRED RESPONSE 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold121G001160 PE=4 SV=1)

HSP 1 Score: 240.7 bits (613), Expect = 3.4e-60
Identity = 119/147 (80.95%), Postives = 120/147 (81.63%), Query Frame = 0

Query: 1   MSTDRSIPKEIVHKTRTNLGVNISYQKPWRAKEHMVKILHGDTVESYALIQRFFDKL--- 60
           MSTDRSIPKEIVHK  TNLGVNISYQK WR KEHMVKILH D VESYALI RFFDKL   
Sbjct: 19  MSTDRSIPKEIVHKAHTNLGVNISYQKAWRVKEHMVKILHDDKVESYALIPRFFDKLVES 78

Query: 61  ----------DDSGHFKFYFMAFDASIEGWKYCRPIISIDGTFLKCKFGGILLIASSQDG 120
                     DDSGHFKF FMAF ASIEGWKYCR IIS+DGTFLKCKFGGILL ASSQDG
Sbjct: 79  NPSTCTALEMDDSGHFKFCFMAFGASIEGWKYCRTIISVDGTFLKCKFGGILLAASSQDG 138

Query: 121 NNQIFPLAFAIVDSENDVSWTWFFEKI 135
           NNQIFPLAF IVD END SWTWFFEKI
Sbjct: 139 NNQIFPLAFVIVDFENDASWTWFFEKI 165

BLAST of Cmc08g0228421 vs. ExPASy TrEMBL
Match: A0A5D3BUA2 (MULE domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold155G00850 PE=4 SV=1)

HSP 1 Score: 240.0 bits (611), Expect = 5.8e-60
Identity = 119/147 (80.95%), Postives = 121/147 (82.31%), Query Frame = 0

Query: 1   MSTDRSIPKEIVHKTRTNLGVNISYQKPWRAKEHMVKILHGDTVESYALIQRFFDKL--- 60
           MSTDRSIPKEIVHK  TNLGVN SYQK WRAKEHMVKILHGDTVE YALI RFFDKL   
Sbjct: 135 MSTDRSIPKEIVHKACTNLGVNTSYQKAWRAKEHMVKILHGDTVELYALIPRFFDKLVES 194

Query: 61  ----------DDSGHFKFYFMAFDASIEGWKYCRPIISIDGTFLKCKFGGILLIASSQDG 120
                     DD+GHFKF FM F ASIEGWKYCRPIIS+D TFLKCKFGGI L ASSQDG
Sbjct: 195 NPGTCTALEMDDNGHFKFCFMDFGASIEGWKYCRPIISVDRTFLKCKFGGIRLTASSQDG 254

Query: 121 NNQIFPLAFAIVDSENDVSWTWFFEKI 135
           NNQIFPLAFAIVDSEND SWTWFFEKI
Sbjct: 255 NNQIFPLAFAIVDSENDASWTWFFEKI 281

BLAST of Cmc08g0228421 vs. ExPASy TrEMBL
Match: A0A5D3BGP1 (MULE domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold180G00990 PE=4 SV=1)

HSP 1 Score: 236.1 bits (601), Expect = 8.4e-59
Identity = 117/147 (79.59%), Postives = 120/147 (81.63%), Query Frame = 0

Query: 1   MSTDRSIPKEIVHKTRTNLGVNISYQKPWRAKEHMVKILHGDTVESYALIQRFFDKL--- 60
           MSTDRSIPKEIVHK RT LGV ISYQK WR KEH+VKILHGDTVESYALI RFFDKL   
Sbjct: 1   MSTDRSIPKEIVHKARTKLGVKISYQKAWRTKEHIVKILHGDTVESYALILRFFDKLVES 60

Query: 61  ----------DDSGHFKFYFMAFDASIEGWKYCRPIISIDGTFLKCKFGGILLIASSQDG 120
                     DDSGHFKF FMAF AS+EGWKYCR IISIDG FLKCKFGGILL ASSQDG
Sbjct: 61  NPGTCTALEMDDSGHFKFCFMAFGASVEGWKYCRSIISIDGIFLKCKFGGILLTASSQDG 120

Query: 121 NNQIFPLAFAIVDSENDVSWTWFFEKI 135
           N+QIFPLAFAIVDSE D  WTWFFEKI
Sbjct: 121 NDQIFPLAFAIVDSEKDALWTWFFEKI 147

BLAST of Cmc08g0228421 vs. ExPASy TrEMBL
Match: A0A5D3BVN2 (Protein FAR1-RELATED SEQUENCE 4 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold968G00010 PE=4 SV=1)

HSP 1 Score: 230.3 bits (586), Expect = 4.6e-57
Identity = 113/141 (80.14%), Postives = 118/141 (83.69%), Query Frame = 0

Query: 1   MSTDRSIPKEIVHKTRTNLGVNISYQKPWRAKEHMVKILHGDTVESYALIQRFFDKL--- 60
           MST+RSIP EIVHK RTNLG+NISYQK WRAKEHMVKILHGDTVESYALI RFFDKL   
Sbjct: 19  MSTNRSIPDEIVHKARTNLGINISYQKAWRAKEHMVKILHGDTVESYALIPRFFDKLVES 78

Query: 61  ----------DDSGHFKFYFMAFDASIEGWKYCRPIISIDGTFLKCKFGGILLIASSQDG 120
                     DDSGHFKF FMAF ASIEGWKYC+PII +DG FLKCKFGGILL ASSQDG
Sbjct: 79  NPGTCTALEMDDSGHFKFCFMAFGASIEGWKYCKPIIYVDGKFLKCKFGGILLTASSQDG 138

Query: 121 NNQIFPLAFAIVDSENDVSWT 129
           NNQIFPLAF IVDS+NDVSWT
Sbjct: 139 NNQIFPLAFTIVDSKNDVSWT 159

BLAST of Cmc08g0228421 vs. TAIR 10
Match: AT1G64260.1 (MuDR family transposase )

HSP 1 Score: 70.5 bits (171), Expect = 1.2e-12
Identity = 34/76 (44.74%), Postives = 46/76 (60.53%), Query Frame = 0

Query: 59  DSGHFKFYFMAFDASIEGWKYCRPIISIDGTFLKCKFGGILLIASSQDGNNQIFPLAFAI 118
           D   F+  F +F  SIEG+++CRP+I +D   L  K+   L+IAS  D  N+ FPLAFA+
Sbjct: 354 DFASFRGVFWSFSQSIEGFQHCRPLIVVDTKSLNGKYQLKLMIASGVDAANKFFPLAFAV 413

Query: 119 VDSENDVSWTWFFEKI 135
               +  SW WFF KI
Sbjct: 414 TKEVSTDSWRWFFTKI 429

BLAST of Cmc08g0228421 vs. TAIR 10
Match: AT1G49920.1 (MuDR family transposase )

HSP 1 Score: 67.0 bits (162), Expect = 1.3e-11
Identity = 38/113 (33.63%), Postives = 61/113 (53.98%), Query Frame = 0

Query: 25  YQKPWRAKEHMVKILHGDTVESYALIQRFFDKL---DDSGHFKFYFMAFDASIEGWKYCR 84
           + + +R    ++ +LH     +  L+   +D L    +   F+  F AF  SI+G+++CR
Sbjct: 323 WDQSFRLIPKLMSVLHS---SNGLLVDWQYDSLTHDPEHASFRGLFWAFSQSIQGFQHCR 382

Query: 85  PIISIDGTFLKCKFGGILLIASSQDGNNQIFPLAFAIVDSENDVSWTWFFEKI 135
           P+I +D   L  K+   L+IAS+ D  NQ FPLAFA+    +  SW WF  +I
Sbjct: 383 PLIVVDTKNLGGKYKMKLMIASAFDATNQYFPLAFAVTKEVSVDSWRWFLTRI 432

BLAST of Cmc08g0228421 vs. TAIR 10
Match: AT1G64255.1 (MuDR family transposase )

HSP 1 Score: 63.9 bits (154), Expect = 1.1e-10
Identity = 30/68 (44.12%), Postives = 41/68 (60.29%), Query Frame = 0

Query: 67  FMAFDASIEGWKYCRPIISIDGTFLKCKFGGILLIASSQDGNNQIFPLAFAIVDSENDVS 126
           F AF  SIEG+++CRP+I +D   L C++   L+IAS  D  N+ FPLAFA+    +   
Sbjct: 369 FWAFPQSIEGFQHCRPLIVVDTKNLNCEYQLKLMIASGVDAANKYFPLAFAVTKEVSTDI 428

Query: 127 WTWFFEKI 135
           W WF   I
Sbjct: 429 WRWFLTGI 436

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAA0036500.14.9e-6183.67protein FAR1-RELATED SEQUENCE 4 [Cucumis melo var. makuwa] >TYJ99981.1 protein F... [more]
KAA0035384.17.1e-6080.95protein FAR-RED IMPAIRED RESPONSE 1-like [Cucumis melo var. makuwa] >TYK03496.1 ... [more]
KAA0047748.11.2e-5980.95uncharacterized protein E6C27_scaffold1059G00070 [Cucumis melo var. makuwa] >TYK... [more]
KAA0050206.11.7e-5879.59uncharacterized protein E6C27_scaffold355G00380 [Cucumis melo var. makuwa] >TYJ9... [more]
TYK02872.19.6e-5780.14protein FAR1-RELATED SEQUENCE 4 [Cucumis melo var. makuwa][more]
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A5D3BMN42.4e-6183.67Protein FAR1-RELATED SEQUENCE 4 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_... [more]
A0A5D3BZ763.4e-6080.95Protein FAR-RED IMPAIRED RESPONSE 1-like OS=Cucumis melo var. makuwa OX=1194695 ... [more]
A0A5D3BUA25.8e-6080.95MULE domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_s... [more]
A0A5D3BGP18.4e-5979.59MULE domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_s... [more]
A0A5D3BVN24.6e-5780.14Protein FAR1-RELATED SEQUENCE 4 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_... [more]
Match NameE-valueIdentityDescription
AT1G64260.11.2e-1244.74MuDR family transposase [more]
AT1G49920.11.3e-1133.63MuDR family transposase [more]
AT1G64255.11.1e-1044.12MuDR family transposase [more]
InterPro
Analysis Name: InterPro Annotations of Melon (Charmono) v1.1
Date Performed: 2022-10-13
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR018289MULE transposase domainPFAMPF10551MULEcoord: 83..133
e-value: 2.2E-10
score: 40.8
NoneNo IPR availablePANTHERPTHR31973POLYPROTEIN, PUTATIVE-RELATEDcoord: 7..133

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cmc08g0228421.1Cmc08g0228421.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0015074 DNA integration
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0008270 zinc ion binding