Cmc08g0227761 (gene) Melon (Charmono) v1.1

Overview
NameCmc08g0227761
Typegene
OrganismCucumis melo L. var. cantalupensis cv. Charmono (Melon (Charmono) v1.1)
DescriptionRetrovirus-related Pol polyprotein from transposon TNT 1-94
LocationCMiso1.1chr08: 21084441 .. 21085316 (-)
RNA-Seq ExpressionCmc08g0227761
SyntenyCmc08g0227761
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTTCAAAGTCTTTGTAACTGAAATAGAGAACCAATTTAACAAAAGAATTAAGAGACTTCATAGTGATAGAGGAACTGAATATGATTCAGTTGCTTTCAATGAATTTTATAACTCAAAAGGAATAATACATAAAACTACTGCGTCTTATTCTACTGAAATGAATGGAAAAGCAGAAAGAAAGAATAGAACTCTAACTAAGTTAGTAGTTGCTATCTTACTTGAGTCAGGAGCAGCACCATCTTGGTGGGGTGAAATAATTAAGACTGTTAATTATGTTCTTAATAGAATTCCTAAATCTAACAGTAAAACTTCACCATACGAAGTCCTTAAACATAAAACACCAAACGTGACTTATCTTAGAACTTGGGGTTGTCTAGCTTATGTTAGAATACCTGATCCAAAAAGAAGGAAATTAGTAAGTAGAGTCTATGGATGTGTCTTCATAGGATACACTGAAAATAGTAAAGCCTATAGATTCTATGACTTAGAAAACAAAGTAATTATAGAATCGAATGACGTAGATTTTTTCAAGGACAGATTTCCTTTTAAATCTAGAAATAGTGGGGGCCTATATAGTCAAACTAGTGGGGGCTCAAGTTCCAGTAGTCTACCTTCAATTAGGATCCAAACCCAAGACAAGGAAGTAGATCCTGAACCTAGAAGAAGCAAGAGAGCTAGAACAATAAAAGACTTCGGAGAAGACTTTGAAATGTACAACGTAGAAGATCCAAAAGATCTAACAGAAGCATTATCATCAGTAGATGCCAATTTATGGCAAGAAGCTATCAATGATGAAATGGACTCTCTTGAATCCAATAGAACTTGGCACCTAGTTGACTTACCCCCTGGATGTAAAGGTATAGGCTGCAAATGA

mRNA sequence

ATGTTCAAAGTCTTTGTAACTGAAATAGAGAACCAATTTAACAAAAGAATTAAGAGACTTCATAGTGATAGAGGAACTGAATATGATTCAGTTGCTTTCAATGAATTTTATAACTCAAAAGGAATAATACATAAAACTACTGCGTCTTATTCTACTGAAATGAATGGAAAAGCAGAAAGAAAGAATAGAACTCTAACTAAGTTAGTAGTTGCTATCTTACTTGAGTCAGGAGCAGCACCATCTTGGTGGGGTGAAATAATTAAGACTGTTAATTATGTTCTTAATAGAATTCCTAAATCTAACAGTAAAACTTCACCATACGAAGTCCTTAAACATAAAACACCAAACGTGACTTATCTTAGAACTTGGGGTTGTCTAGCTTATGTTAGAATACCTGATCCAAAAAGAAGGAAATTAGTAAGTAGAGTCTATGGATGTGTCTTCATAGGATACACTGAAAATAGTAAAGCCTATAGATTCTATGACTTAGAAAACAAAGTAATTATAGAATCGAATGACGTAGATTTTTTCAAGGACAGATTTCCTTTTAAATCTAGAAATAGTGGGGGCCTATATAGTCAAACTAGTGGGGGCTCAAGTTCCAGTAGTCTACCTTCAATTAGGATCCAAACCCAAGACAAGGAAGTAGATCCTGAACCTAGAAGAAGCAAGAGAGCTAGAACAATAAAAGACTTCGGAGAAGACTTTGAAATGTACAACGTAGAAGATCCAAAAGATCTAACAGAAGCATTATCATCAGTAGATGCCAATTTATGGCAAGAAGCTATCAATGATGAAATGGACTCTCTTGAATCCAATAGAACTTGGCACCTAGTTGACTTACCCCCTGGATGTAAAGGTATAGGCTGCAAATGA

Coding sequence (CDS)

ATGTTCAAAGTCTTTGTAACTGAAATAGAGAACCAATTTAACAAAAGAATTAAGAGACTTCATAGTGATAGAGGAACTGAATATGATTCAGTTGCTTTCAATGAATTTTATAACTCAAAAGGAATAATACATAAAACTACTGCGTCTTATTCTACTGAAATGAATGGAAAAGCAGAAAGAAAGAATAGAACTCTAACTAAGTTAGTAGTTGCTATCTTACTTGAGTCAGGAGCAGCACCATCTTGGTGGGGTGAAATAATTAAGACTGTTAATTATGTTCTTAATAGAATTCCTAAATCTAACAGTAAAACTTCACCATACGAAGTCCTTAAACATAAAACACCAAACGTGACTTATCTTAGAACTTGGGGTTGTCTAGCTTATGTTAGAATACCTGATCCAAAAAGAAGGAAATTAGTAAGTAGAGTCTATGGATGTGTCTTCATAGGATACACTGAAAATAGTAAAGCCTATAGATTCTATGACTTAGAAAACAAAGTAATTATAGAATCGAATGACGTAGATTTTTTCAAGGACAGATTTCCTTTTAAATCTAGAAATAGTGGGGGCCTATATAGTCAAACTAGTGGGGGCTCAAGTTCCAGTAGTCTACCTTCAATTAGGATCCAAACCCAAGACAAGGAAGTAGATCCTGAACCTAGAAGAAGCAAGAGAGCTAGAACAATAAAAGACTTCGGAGAAGACTTTGAAATGTACAACGTAGAAGATCCAAAAGATCTAACAGAAGCATTATCATCAGTAGATGCCAATTTATGGCAAGAAGCTATCAATGATGAAATGGACTCTCTTGAATCCAATAGAACTTGGCACCTAGTTGACTTACCCCCTGGATGTAAAGGTATAGGCTGCAAATGA

Protein sequence

MFKVFVTEIENQFNKRIKRLHSDRGTEYDSVAFNEFYNSKGIIHKTTASYSTEMNGKAERKNRTLTKLVVAILLESGAAPSWWGEIIKTVNYVLNRIPKSNSKTSPYEVLKHKTPNVTYLRTWGCLAYVRIPDPKRRKLVSRVYGCVFIGYTENSKAYRFYDLENKVIIESNDVDFFKDRFPFKSRNSGGLYSQTSGGSSSSSLPSIRIQTQDKEVDPEPRRSKRARTIKDFGEDFEMYNVEDPKDLTEALSSVDANLWQEAINDEMDSLESNRTWHLVDLPPGCKGIGCK
Homology
BLAST of Cmc08g0227761 vs. NCBI nr
Match: KAA0034938.1 (putative Polyprotein [Cucumis melo var. makuwa] >TYK21293.1 putative Polyprotein [Cucumis melo var. makuwa])

HSP 1 Score: 525.4 bits (1352), Expect = 3.1e-145
Identity = 257/285 (90.18%), Postives = 269/285 (94.39%), Query Frame = 0

Query: 1   MFKVFVTEIENQFNKRIKRLHSDRGTEYDSVAFNEFYNSKGIIHKTTASYSTEMNGKAER 60
           MFKVFVTEIENQFNKRIKRL SDRGTEYDSVAFNEFYNSKGIIH+TT  YS EMNGK ER
Sbjct: 403 MFKVFVTEIENQFNKRIKRLRSDRGTEYDSVAFNEFYNSKGIIHETTTPYSPEMNGKEER 462

Query: 61  KNRTLTKLVVAILLESGAAPSWWGEIIKTVNYVLNRIPKSNSKTSPYEVLKHKTPNVTYL 120
           KNRTLT+L VAILLES AAPSWWGEIIKTVNYVLNRIPKSNSKTSPYEVLKHK PN++YL
Sbjct: 463 KNRTLTELAVAILLESEAAPSWWGEIIKTVNYVLNRIPKSNSKTSPYEVLKHKIPNLSYL 522

Query: 121 RTWGCLAYVRIPDPKRRKLVSRVYGCVFIGYTENSKAYRFYDLENKVIIESNDVDFFKDR 180
           RTWGCLAYVRIP+P+RRKL S+ Y CVFIGY ENSKAYRFYDLENKVIIESNDVDFF+D+
Sbjct: 523 RTWGCLAYVRIPNPERRKLASKAYECVFIGYAENSKAYRFYDLENKVIIESNDVDFFEDK 582

Query: 181 FPFKSRNSGGLYSQTSGGSSSSSLPSIRIQTQDKEVDPEPRRSKRARTIKDFGEDFEMYN 240
           FPFKSRNSGGLYSQTSGGSS SSLPSIRIQTQDKEVDPEPRRSKRART+KDF EDFEMYN
Sbjct: 583 FPFKSRNSGGLYSQTSGGSSFSSLPSIRIQTQDKEVDPEPRRSKRARTVKDFREDFEMYN 642

Query: 241 VEDPKDLTEALSSVDANLWQEAINDEMDSLESNRTWHLVDLPPGC 286
           VEDPKDLT+ALSSVDANLWQEAIND +DSLESNRTWHLVDLPP C
Sbjct: 643 VEDPKDLTKALSSVDANLWQEAINDGIDSLESNRTWHLVDLPPRC 687

BLAST of Cmc08g0227761 vs. NCBI nr
Match: RZC09450.1 (Retrovirus-related Pol polyprotein from transposon TNT 1-94 [Glycine soja])

HSP 1 Score: 406.8 bits (1044), Expect = 1.6e-109
Identity = 204/293 (69.62%), Postives = 234/293 (79.86%), Query Frame = 0

Query: 1   MFKVFVTEIENQFNKRIKRLHSDRGTEYDSVAFNEFYNSKGIIHKTTASYSTEMNGKAER 60
           MFK+FVTEIENQFNK+IK+L SDRGT+YDS  FNEFYN  GIIH+TTA YS EMNGKAER
Sbjct: 10  MFKLFVTEIENQFNKKIKKLRSDRGTKYDSSLFNEFYNLHGIIHETTAPYSPEMNGKAER 69

Query: 61  KNRTLTKLVVAILLESGAAPSWWGEIIKTVNYVLNRIPKSNSKTSPYEVLKHKTPNVTYL 120
           KNRT T+LVVA +L S A   WWGEI+ TV YVLNRIPKS SKTSPYE+LK + PN++YL
Sbjct: 70  KNRTFTELVVATMLSSSATSFWWGEILLTVCYVLNRIPKSKSKTSPYEILKKRQPNLSYL 129

Query: 121 RTWGCLAYVRIPDPKRRKLVSRVYGCVFIGYTENSKAYRFYDLENKVIIESNDVDFFKDR 180
           RTWGCLAYVRIPDPKR KL SR Y CVFIGY  NSKAYRFYDL  KVIIESND DF++++
Sbjct: 130 RTWGCLAYVRIPDPKRVKLASRAYECVFIGYAINSKAYRFYDLNAKVIIESNDADFYENK 189

Query: 181 FPFKSRNSGGLYSQTSGGSSSSSLPSIRIQT-QDKEVDPEPRRSKRARTIKDFGEDFEMY 240
           FPFK R+        SGG+SS+ LP+I  +     + D EPRR KRAR  KD+G D+  Y
Sbjct: 190 FPFKLRD--------SGGTSSNYLPAISSENLAQPKPDIEPRRGKRARIAKDYGPDYMAY 249

Query: 241 NV-EDPKDLTEALSSVDANLWQEAINDEMDSLESNRTWHLVDLPPGCKGIGCK 292
            + EDP +L EALS +DA+LWQEAINDEMDSLES++TWHLVDLPPGCK IGCK
Sbjct: 250 TLEEDPSNLQEALSFLDADLWQEAINDEMDSLESDKTWHLVDLPPGCKPIGCK 294

BLAST of Cmc08g0227761 vs. NCBI nr
Match: ABI34306.1 (Polyprotein, putative [Solanum demissum])

HSP 1 Score: 307.4 bits (786), Expect = 1.3e-79
Identity = 161/293 (54.95%), Postives = 206/293 (70.31%), Query Frame = 0

Query: 2   FKVFVTEIENQFNKRIKRLHSDRGTEYDSVAFNEFYNSKGIIHKTTASYSTEMNGKAERK 61
           FK ++ E+ENQF ++IKR+ SDRG EY+S  FN F  S GIIH+TT  YS   NG AERK
Sbjct: 543 FKTYLHEVENQFGRKIKRIRSDRGREYESNEFNSFVRSLGIIHETTPPYSPSSNGVAERK 602

Query: 62  NRTLTKLVVAILLESGAAPSWWGEIIKTVNYVLNRIPKSNSKTSPYEVLKHKTPNVTYLR 121
           NRTL +L  A+L+ES A  ++WGE I T  YVLNR+P   SK +P+E+ K   P++ YLR
Sbjct: 603 NRTLVELTNAMLIESHAPLNFWGEAILTACYVLNRVPHKKSKLTPFELWKGYKPSLGYLR 662

Query: 122 TWGCLAYVRIPDPKRRKLVSRVYGCVFIGYTENSKAYRFYDLENKVIIESNDVDFFKDRF 181
            WGCLA+VR+ DPK  KL  +V  C F+GY  NS AYRF++LE+ ++IES D  F +++F
Sbjct: 663 VWGCLAFVRLMDPKITKLGKKVTTCAFLGYASNSTAYRFFNLEDNIVIESGDAIFHENKF 722

Query: 182 PFKSRNSGGLYSQTSGGSSSSSLPSIRIQT-QDKEV-DPEPRRSKRARTIKDFGEDFEMY 241
           PF S+NSGG   +     +  +LPS    T ++KEV D E RRSKRAR  KDFG DF ++
Sbjct: 723 PFDSKNSGGQRIE----QNILTLPSSSTSTLKNKEVNDFELRRSKRARVEKDFGPDFYVF 782

Query: 242 NVEDPK-DLTEALSSVDANLWQEAINDEMDSLESNRTWHLVDLPPGCKGIGCK 292
           NV D +  L EALSS D+  W+EA+NDEM+SL SN+TW LVDLPPGCK IGCK
Sbjct: 783 NVGDDRLTLKEALSSHDSIFWKEAVNDEMESLISNKTWKLVDLPPGCKTIGCK 831

BLAST of Cmc08g0227761 vs. NCBI nr
Match: AAU90333.1 (Putative gag and pol polyprotein, identical [Solanum demissum])

HSP 1 Score: 306.2 bits (783), Expect = 3.0e-79
Identity = 161/293 (54.95%), Postives = 206/293 (70.31%), Query Frame = 0

Query: 2   FKVFVTEIENQFNKRIKRLHSDRGTEYDSVAFNEFYNSKGIIHKTTASYSTEMNGKAERK 61
           FK ++ E+ENQF ++IKR+ SDRG EY+S  FN F  S GIIH+TT  YS   NG AERK
Sbjct: 344 FKTYLHEVENQFGRKIKRIRSDRGREYESNEFNSFVRSLGIIHETTPPYSPSSNGAAERK 403

Query: 62  NRTLTKLVVAILLESGAAPSWWGEIIKTVNYVLNRIPKSNSKTSPYEVLKHKTPNVTYLR 121
           NRTL +L  A+L+ES A  ++WGE I T  YVLNR+P   SK + +E+ K   P++ YLR
Sbjct: 404 NRTLVELTNAMLIESHAPLNFWGETILTACYVLNRVPHKKSKLTHFELWKGYKPSLGYLR 463

Query: 122 TWGCLAYVRIPDPKRRKLVSRVYGCVFIGYTENSKAYRFYDLENKVIIESNDVDFFKDRF 181
            WGCLA+VR+ DPK  KL  +V  C F+GY  NS AYRF++LE+ ++IES D  F +++F
Sbjct: 464 VWGCLAFVRLMDPKITKLGKKVTTCAFLGYASNSTAYRFFNLEDNIVIESGDAIFHENKF 523

Query: 182 PFKSRNSGGLYSQTSGGSSSSSLPSIRIQT-QDKEV-DPEPRRSKRARTIKDFGEDFEMY 241
           PF S+NSGG   +     +  SLPS    T ++KEV D E RRSKRAR  KDFG +F ++
Sbjct: 524 PFDSKNSGGQRIE----QNILSLPSSSTSTLKNKEVNDFELRRSKRARIEKDFGPNFYVF 583

Query: 242 NV-EDPKDLTEALSSVDANLWQEAINDEMDSLESNRTWHLVDLPPGCKGIGCK 292
           NV +DP  L EALSS D+  W+EA+NDEM+SL SN+TW LVDLPPGCK IGCK
Sbjct: 584 NVGDDPLTLKEALSSHDSIFWKEAVNDEMESLISNKTWKLVDLPPGCKTIGCK 632

BLAST of Cmc08g0227761 vs. NCBI nr
Match: XP_023158131.2 (uncharacterized protein LOC103653943 isoform X1 [Zea mays] >XP_035823266.1 uncharacterized protein LOC103653943 isoform X1 [Zea mays])

HSP 1 Score: 280.0 bits (715), Expect = 2.3e-71
Identity = 148/298 (49.66%), Postives = 192/298 (64.43%), Query Frame = 0

Query: 2   FKVFVTEIENQFNKRIKRLHSDRGTEYDSVAFNEFYNSKGIIHKTTASYSTEMNGKAERK 61
           FK++ TE+ENQ +K+IKRL SDRG EY S  F+E+    GIIH+TTA YS + NG AERK
Sbjct: 623 FKIYKTEVENQLDKKIKRLRSDRGGEYLSNLFDEYCKECGIIHETTAPYSPQSNGVAERK 682

Query: 62  NRTLTKLVVAILLESGAAPSWWGEIIKTVNYVLNRIPKSNSKTSPYEVLKHKTPNVTYLR 121
           NRT+  L  A+L  SG    WWGE + TV YVLNR+P  N + +PYE  K + P++++LR
Sbjct: 683 NRTVCDLANALLQSSGMPDIWWGEAVLTVCYVLNRVPPRNREATPYEGFKGRKPDLSHLR 742

Query: 122 TWGCLAYVRIPDPKRRKLVSRVYGCVFIGYTENSKAYRFYDLENK-------VIIESNDV 181
           TWGCLA V +P PK+RKL  +   CVF+GY  NS AYRF  + ++       VI+ES DV
Sbjct: 743 TWGCLAKVNVPLPKKRKLGPKTVDCVFLGYAHNSAAYRFLVVHSETSEVAINVIMESRDV 802

Query: 182 DFFKDRFPFKSRNSGGLYSQTSGGSSSSSLPSIRIQTQDKEVDPEPRRSKRARTIKDFGE 241
            FF+  FP + +          G S + SLPS      D+  D E RRSKR RT K  G+
Sbjct: 803 TFFESIFPMRDKE----VVAPDGPSRTYSLPS---SVNDQTPDLELRRSKRQRTEKSLGD 862

Query: 242 DFEMYNV-EDPKDLTEALSSVDANLWQEAINDEMDSLESNRTWHLVDLPPGCKGIGCK 292
           D+ +Y V E+P+ LTEA +S DA  W+EA+  EMDS+ SN TW + DLP GCK +GCK
Sbjct: 863 DYIIYLVDEEPRSLTEAYTSPDAEYWREAVRSEMDSIISNGTWEITDLPAGCKPVGCK 913

BLAST of Cmc08g0227761 vs. ExPASy Swiss-Prot
Match: P10978 (Retrovirus-related Pol polyprotein from transposon TNT 1-94 OS=Nicotiana tabacum OX=4097 PE=2 SV=1)

HSP 1 Score: 144.4 bits (363), Expect = 2.0e-33
Identity = 101/331 (30.51%), Postives = 154/331 (46.53%), Query Frame = 0

Query: 1   MFKVFVTEIENQFNKRIKRLHSDRGTEYDSVAFNEFYNSKGIIHKTTASYSTEMNGKAER 60
           +F+ F   +E +  +++KRL SD G EY S  F E+ +S GI H+ T   + + NG AER
Sbjct: 528 VFQKFHALVERETGRKLKRLRSDNGGEYTSREFEEYCSSHGIRHEKTVPGTPQHNGVAER 587

Query: 61  KNRTLTKLVVAILLESGAAPSWWGEIIKTVNYVLNRIPK-SNSKTSPYEVLKHKTPNVTY 120
            NRT+ + V ++L  +    S+WGE ++T  Y++NR P    +   P  V  +K  + ++
Sbjct: 588 MNRTIVEKVRSMLRMAKLPKSFWGEAVQTACYLINRSPSVPLAFEIPERVWTNKEVSYSH 647

Query: 121 LRTWGCLAYVRIPDPKRRKLVSRVYGCVFIGYTENSKAYRFYDLENKVIIESNDVDFFKD 180
           L+ +GC A+  +P  +R KL  +   C+FIGY +    YR +D   K +I S DV F + 
Sbjct: 648 LKVFGCRAFAHVPKEQRTKLDDKSIPCIFIGYGDEEFGYRLWDPVKKKVIRSRDVVFRES 707

Query: 181 R---------------------FPFKSRNSGGLYSQTSGGSSSSSLPSIRIQ-------- 240
                                  P  S N     S T   S     P   I+        
Sbjct: 708 EVRTAADMSEKVKNGIIPNFVTIPSTSNNPTSAESTTDEVSEQGEQPGEVIEQGEQLDEG 767

Query: 241 -------TQDKEVDPEPRRSKRARTIKDFGEDFEMYNVED---PKDLTEALSSVDANLWQ 292
                  TQ +E     RRS+R R         E   + D   P+ L E LS  + N   
Sbjct: 768 VEEVEHPTQGEEQHQPLRRSERPRVESRRYPSTEYVLISDDREPESLKEVLSHPEKNQLM 827

BLAST of Cmc08g0227761 vs. ExPASy Swiss-Prot
Match: P04146 (Copia protein OS=Drosophila melanogaster OX=7227 GN=GIP PE=1 SV=3)

HSP 1 Score: 101.7 bits (252), Expect = 1.5e-20
Identity = 79/281 (28.11%), Postives = 137/281 (48.75%), Query Frame = 0

Query: 1   MFKVFVTEIENQFNKRIKRLHSDRGTEYDSVAFNEFYNSKGIIHKTTASYSTEMNGKAER 60
           MF+ FV + E  FN ++  L+ D G EY S    +F   KGI +  T  ++ ++NG +ER
Sbjct: 528 MFQDFVAKSEAHFNLKVVYLYIDNGREYLSNEMRQFCVKKGISYHLTVPHTPQLNGVSER 587

Query: 61  KNRTLTKLVVAILLESGAAPSWWGEIIKTVNYVLNRIPKS---NSKTSPYEVLKHKTPNV 120
             RT+T+    ++  +    S+WGE + T  Y++NRIP     +S  +PYE+  +K P +
Sbjct: 588 MIRTITEKARTMVSGAKLDKSFWGEAVLTATYLINRIPSRALVDSSKTPYEMWHNKKPYL 647

Query: 121 TYLRTWGCLAYVRIPDPKRRKLVSRVYGCVFIGYTENSKAYRFYDLENKVIIESNDVDFF 180
            +LR +G   YV I + K+ K   + +  +F+GY  N   ++ +D  N+  I + DV   
Sbjct: 648 KHLRVFGATVYVHIKN-KQGKFDDKSFKSIFVGYEPN--GFKLWDAVNEKFIVARDVVVD 707

Query: 181 K-DRFPFKSRNSGGLYSQTSGGSSSSSLPSIRIQTQDKEVDPE-PRRSKRARTIKDFGED 240
           + +    ++     ++ + S  S + + P+       K +  E P  SK    I+   + 
Sbjct: 708 ETNMVNSRAVKFETVFLKDSKESENKNFPN----DSRKIIQTEFPNESKECDNIQFLKDS 767

Query: 241 FEMYNVEDPKDLTEALSSVDANLWQEAINDEM--DSLESNR 275
            E  N   P D  + + +   N  +E  N +   DS ESN+
Sbjct: 768 KESENKNFPNDSRKIIQTEFPNESKECDNIQFLKDSKESNK 801

BLAST of Cmc08g0227761 vs. ExPASy Swiss-Prot
Match: P0C2J7 (Transposon Ty4-H Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY4B-H PE=3 SV=1)

HSP 1 Score: 64.7 bits (156), Expect = 2.0e-09
Identity = 51/249 (20.48%), Postives = 103/249 (41.37%), Query Frame = 0

Query: 9   IENQFNKRIKRLHSDRGTEYDSVAFNEFYNSKGIIHKTTASYSTEMNGKAERKNRTLTKL 68
           +E QF+++++ ++SDRGTE+ +    E++ SKGI H  T++     NG+AER  RT+   
Sbjct: 681 VETQFDRKVREINSDRGTEFTNDQIEEYFISKGIHHILTSTQDHAANGRAERYIRTIVTD 740

Query: 69  VVAILLESGAAPSWWGEIIKTVNYVLNRIPKSNSKTSPYEVLKHKTPNVTYLRTWGCLAY 128
              +L +S     +W   + +   + N +   ++   P + +  +   V  +        
Sbjct: 741 ATTLLRQSNLRVKFWEYAVTSATNIRNCLEHKSTGKLPLKAISRQPVTVRLMSFLPFGEK 800

Query: 129 VRIPDPKRRKLVSRVYGCVFIGYTENSKAYRFYDLENKVIIESNDVDFFKDRFPFKSRNS 188
             I +   +KL       + +    NS  Y+F+      I+ S++          + RN+
Sbjct: 801 GIIWNHNHKKLKPSGLPSIILCKDPNSYGYKFFIPSKNKIVTSDNYTIPNYTMDGRVRNT 860

Query: 189 GGLYSQTSGGSSSSSLPSIRIQTQDKEVDPEPRRSKRARTIKDFGEDFEMYNVEDPKDLT 248
             +Y      S +           D E D     +     ++++ +D +     +     
Sbjct: 861 QNIYKSHQFSSHN-----------DNEEDQIETVTNLCEALENYEDDNKPITRLEDLFTE 918

Query: 249 EALSSVDAN 258
           E LS +D+N
Sbjct: 921 EELSQIDSN 918

BLAST of Cmc08g0227761 vs. ExPASy Swiss-Prot
Match: A0A0B7P3V8 (Transposon Ty4-P Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY4B-P PE=5 SV=2)

HSP 1 Score: 64.7 bits (156), Expect = 2.0e-09
Identity = 51/249 (20.48%), Postives = 103/249 (41.37%), Query Frame = 0

Query: 9   IENQFNKRIKRLHSDRGTEYDSVAFNEFYNSKGIIHKTTASYSTEMNGKAERKNRTLTKL 68
           +E QF+++++ ++SDRGTE+ +    E++ SKGI H  T++     NG+AER  RT+   
Sbjct: 681 VETQFDRKVREINSDRGTEFTNDQIEEYFISKGIHHILTSTQDHAANGRAERYIRTIVTD 740

Query: 69  VVAILLESGAAPSWWGEIIKTVNYVLNRIPKSNSKTSPYEVLKHKTPNVTYLRTWGCLAY 128
              +L +S     +W   + +   + N +   ++   P + +  +   V  +        
Sbjct: 741 ATTLLRQSNLRVKFWEYAVTSATNIRNCLEHKSTGKLPLKAISRQPVTVRLMSFLPFGEK 800

Query: 129 VRIPDPKRRKLVSRVYGCVFIGYTENSKAYRFYDLENKVIIESNDVDFFKDRFPFKSRNS 188
             I +   +KL       + +    NS  Y+F+      I+ S++          + RN+
Sbjct: 801 GIIWNHNHKKLKPSGLPSIILCKDPNSYGYKFFIPSKNKIVTSDNYTIPNYTMDGRVRNT 860

Query: 189 GGLYSQTSGGSSSSSLPSIRIQTQDKEVDPEPRRSKRARTIKDFGEDFEMYNVEDPKDLT 248
             +Y      S +           D E D     +     ++++ +D +     +     
Sbjct: 861 QNIYKSHQFSSHN-----------DNEEDQIETVTNLCEALENYEDDNKPITRLEDLFTE 918

Query: 249 EALSSVDAN 258
           E LS +D+N
Sbjct: 921 EELSQIDSN 918

BLAST of Cmc08g0227761 vs. ExPASy Swiss-Prot
Match: P47024 (Transposon Ty4-J Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY4B-J PE=3 SV=3)

HSP 1 Score: 62.0 bits (149), Expect = 1.3e-08
Identity = 52/249 (20.88%), Postives = 101/249 (40.56%), Query Frame = 0

Query: 9   IENQFNKRIKRLHSDRGTEYDSVAFNEFYNSKGIIHKTTASYSTEMNGKAERKNRTLTKL 68
           +E QF+++++ ++SDRGTE+ +    E++ SKGI H  T++     NG+AER  RT+   
Sbjct: 682 VETQFDRKVREINSDRGTEFTNDQIEEYFISKGIHHILTSTQDHAANGRAERYIRTIITD 741

Query: 69  VVAILLESGAAPSWWGEIIKTVNYVLNRIPKSNSKTSPYEVLKHKTPNVTYLRTWGCLAY 128
              +L +S     +W   + +   + N +   ++   P + +  +   V  +        
Sbjct: 742 ATTLLRQSNLRVKFWEYAVTSATNIRNYLEHKSTGKLPLKAISRQPVTVRLMSFLPFGEK 801

Query: 129 VRIPDPKRRKLVSRVYGCVFIGYTENSKAYRFYDLENKVIIESNDVDFFKDRFPFKSRNS 188
             I +   +KL       + +    NS  Y+F+      I+ S       D +   +   
Sbjct: 802 GIIWNHNHKKLKPSGLPSIILCKDPNSYGYKFFIPSKNKIVTS-------DNYTIPNYTM 861

Query: 189 GGLYSQTSGGSSSSSLPSIRIQTQDKEVDPEPRRSKRARTIKDFGEDFEMYNVEDPKDLT 248
            G    T   + S    S      D E D     +     ++++ +D +     +     
Sbjct: 862 DGRVRNTQNINKSHQFSS----DNDDEEDQIETVTNLCEALENYEDDNKPITRLEDLFTE 919

Query: 249 EALSSVDAN 258
           E LS +D+N
Sbjct: 922 EELSQIDSN 919

BLAST of Cmc08g0227761 vs. ExPASy TrEMBL
Match: A0A5D3DCJ1 (Putative Polyprotein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1199G00010 PE=4 SV=1)

HSP 1 Score: 525.4 bits (1352), Expect = 1.5e-145
Identity = 257/285 (90.18%), Postives = 269/285 (94.39%), Query Frame = 0

Query: 1   MFKVFVTEIENQFNKRIKRLHSDRGTEYDSVAFNEFYNSKGIIHKTTASYSTEMNGKAER 60
           MFKVFVTEIENQFNKRIKRL SDRGTEYDSVAFNEFYNSKGIIH+TT  YS EMNGK ER
Sbjct: 403 MFKVFVTEIENQFNKRIKRLRSDRGTEYDSVAFNEFYNSKGIIHETTTPYSPEMNGKEER 462

Query: 61  KNRTLTKLVVAILLESGAAPSWWGEIIKTVNYVLNRIPKSNSKTSPYEVLKHKTPNVTYL 120
           KNRTLT+L VAILLES AAPSWWGEIIKTVNYVLNRIPKSNSKTSPYEVLKHK PN++YL
Sbjct: 463 KNRTLTELAVAILLESEAAPSWWGEIIKTVNYVLNRIPKSNSKTSPYEVLKHKIPNLSYL 522

Query: 121 RTWGCLAYVRIPDPKRRKLVSRVYGCVFIGYTENSKAYRFYDLENKVIIESNDVDFFKDR 180
           RTWGCLAYVRIP+P+RRKL S+ Y CVFIGY ENSKAYRFYDLENKVIIESNDVDFF+D+
Sbjct: 523 RTWGCLAYVRIPNPERRKLASKAYECVFIGYAENSKAYRFYDLENKVIIESNDVDFFEDK 582

Query: 181 FPFKSRNSGGLYSQTSGGSSSSSLPSIRIQTQDKEVDPEPRRSKRARTIKDFGEDFEMYN 240
           FPFKSRNSGGLYSQTSGGSS SSLPSIRIQTQDKEVDPEPRRSKRART+KDF EDFEMYN
Sbjct: 583 FPFKSRNSGGLYSQTSGGSSFSSLPSIRIQTQDKEVDPEPRRSKRARTVKDFREDFEMYN 642

Query: 241 VEDPKDLTEALSSVDANLWQEAINDEMDSLESNRTWHLVDLPPGC 286
           VEDPKDLT+ALSSVDANLWQEAIND +DSLESNRTWHLVDLPP C
Sbjct: 643 VEDPKDLTKALSSVDANLWQEAINDGIDSLESNRTWHLVDLPPRC 687

BLAST of Cmc08g0227761 vs. ExPASy TrEMBL
Match: A0A445KFK2 (Retrovirus-related Pol polyprotein from transposon TNT 1-94 OS=Glycine soja OX=3848 GN=D0Y65_015970 PE=4 SV=1)

HSP 1 Score: 406.8 bits (1044), Expect = 7.8e-110
Identity = 204/293 (69.62%), Postives = 234/293 (79.86%), Query Frame = 0

Query: 1   MFKVFVTEIENQFNKRIKRLHSDRGTEYDSVAFNEFYNSKGIIHKTTASYSTEMNGKAER 60
           MFK+FVTEIENQFNK+IK+L SDRGT+YDS  FNEFYN  GIIH+TTA YS EMNGKAER
Sbjct: 10  MFKLFVTEIENQFNKKIKKLRSDRGTKYDSSLFNEFYNLHGIIHETTAPYSPEMNGKAER 69

Query: 61  KNRTLTKLVVAILLESGAAPSWWGEIIKTVNYVLNRIPKSNSKTSPYEVLKHKTPNVTYL 120
           KNRT T+LVVA +L S A   WWGEI+ TV YVLNRIPKS SKTSPYE+LK + PN++YL
Sbjct: 70  KNRTFTELVVATMLSSSATSFWWGEILLTVCYVLNRIPKSKSKTSPYEILKKRQPNLSYL 129

Query: 121 RTWGCLAYVRIPDPKRRKLVSRVYGCVFIGYTENSKAYRFYDLENKVIIESNDVDFFKDR 180
           RTWGCLAYVRIPDPKR KL SR Y CVFIGY  NSKAYRFYDL  KVIIESND DF++++
Sbjct: 130 RTWGCLAYVRIPDPKRVKLASRAYECVFIGYAINSKAYRFYDLNAKVIIESNDADFYENK 189

Query: 181 FPFKSRNSGGLYSQTSGGSSSSSLPSIRIQT-QDKEVDPEPRRSKRARTIKDFGEDFEMY 240
           FPFK R+        SGG+SS+ LP+I  +     + D EPRR KRAR  KD+G D+  Y
Sbjct: 190 FPFKLRD--------SGGTSSNYLPAISSENLAQPKPDIEPRRGKRARIAKDYGPDYMAY 249

Query: 241 NV-EDPKDLTEALSSVDANLWQEAINDEMDSLESNRTWHLVDLPPGCKGIGCK 292
            + EDP +L EALS +DA+LWQEAINDEMDSLES++TWHLVDLPPGCK IGCK
Sbjct: 250 TLEEDPSNLQEALSFLDADLWQEAINDEMDSLESDKTWHLVDLPPGCKPIGCK 294

BLAST of Cmc08g0227761 vs. ExPASy TrEMBL
Match: A0A7N2L531 (Uncharacterized protein OS=Quercus lobata OX=97700 PE=4 SV=1)

HSP 1 Score: 325.5 bits (833), Expect = 2.3e-85
Identity = 168/291 (57.73%), Postives = 208/291 (71.48%), Query Frame = 0

Query: 2   FKVFVTEIENQFNKRIKRLHSDRGTEYDSVAFNEFYNSKGIIHKTTASYSTEMNGKAERK 61
           F+ F+ E+ENQF ++IKR+ SDRG EY+S AFN F  S GIIH+TTA YS   NG AERK
Sbjct: 567 FQDFLQEVENQFGRKIKRIRSDRGREYESSAFNSFAQSLGIIHETTAPYSPASNGVAERK 626

Query: 62  NRTLTKLVVAILLESGAAPSWWGEIIKTVNYVLNRIPKSNSKTSPYEVLKHKTPNVTYLR 121
           NRTL +L  A+L+ESGA   +WGE I T  +VLNR+P   S T+P+E+ K   PN+ YLR
Sbjct: 627 NRTLIELTNAMLIESGAPLHFWGEAILTACHVLNRVPHKKSHTTPFEMWKGHKPNLGYLR 686

Query: 122 TWGCLAYVRIPDPKRRKLVSRVYGCVFIGYTENSKAYRFYDLENKVIIESNDVDFFKDRF 181
            W CLAYVR+ DPK  KL  R   C F+GY  NS AYRF+DLENK+I ES D  F +++F
Sbjct: 687 AWDCLAYVRLTDPKMPKLGIRATTCAFLGYAINSAAYRFFDLENKIIFESGDAIFHEEKF 746

Query: 182 PFKSRNSGGLYSQTSGGSSSSSLPSIRIQTQDKEVDPEPRRSKRARTIKDFGEDFEMYNV 241
           PFK +NSGG  +  S  SSS+S      Q Q+   + EPRRSKRAR  KDFG D+ ++N+
Sbjct: 747 PFKLKNSGGEENILSQPSSSTS----HFQNQE-NFEMEPRRSKRARVEKDFGPDYYVFNI 806

Query: 242 ED-PKDLTEALSSVDANLWQEAINDEMDSLESNRTWHLVDLPPGCKGIGCK 292
           E+ PK+L EAL+S DA  W+EA+NDEM+SL SNRTW LVDLPPGCK IGCK
Sbjct: 807 EENPKNLKEALTSPDAIFWKEAVNDEMESLISNRTWKLVDLPPGCKTIGCK 852

BLAST of Cmc08g0227761 vs. ExPASy TrEMBL
Match: A0A7N2R9F3 (Uncharacterized protein OS=Quercus lobata OX=97700 PE=4 SV=1)

HSP 1 Score: 319.3 bits (817), Expect = 1.6e-83
Identity = 165/291 (56.70%), Postives = 207/291 (71.13%), Query Frame = 0

Query: 2   FKVFVTEIENQFNKRIKRLHSDRGTEYDSVAFNEFYNSKGIIHKTTASYSTEMNGKAERK 61
           F+ F+ E+ENQF ++IKR+ SDRG EY+S AFN F  S GIIH+TTA YS   NG  ERK
Sbjct: 567 FQDFLKEVENQFGRKIKRIRSDRGREYESSAFNSFVQSLGIIHETTAPYSPASNGVVERK 626

Query: 62  NRTLTKLVVAILLESGAAPSWWGEIIKTVNYVLNRIPKSNSKTSPYEVLKHKTPNVTYLR 121
           NRTL +L  A+L+ESGA   +WGE I T  +VLNR+P   S T+P+E+ K   PN+ YLR
Sbjct: 627 NRTLIELTNAMLIESGAPLHFWGEAILTACHVLNRVPHKKSHTTPFEMWKGHKPNLGYLR 686

Query: 122 TWGCLAYVRIPDPKRRKLVSRVYGCVFIGYTENSKAYRFYDLENKVIIESNDVDFFKDRF 181
            WGCLAYVR+ DPK  KL  R   C F+GY  NS AYRF+DLENK+I ES D  F +++F
Sbjct: 687 VWGCLAYVRLTDPKIPKLGIRATTCAFLGYAINSAAYRFFDLENKIIFESGDAIFHEEKF 746

Query: 182 PFKSRNSGGLYSQTSGGSSSSSLPSIRIQTQDKEVDPEPRRSKRARTIKDFGEDFEMYNV 241
           PFK +NSGG  +     SSS+S     +Q Q+   + E RRSKRAR  KDFG D+ ++N+
Sbjct: 747 PFKLKNSGGEENILLQPSSSTS----HLQNQE-NFEMELRRSKRARVEKDFGPDYYVFNI 806

Query: 242 ED-PKDLTEALSSVDANLWQEAINDEMDSLESNRTWHLVDLPPGCKGIGCK 292
           E+ P++L EAL+S DA  W+EA+NDEM+SL SNRTW LVDLPPGCK IGCK
Sbjct: 807 EENPQNLKEALTSSDAIFWKEAVNDEMESLISNRTWKLVDLPPGCKTIGCK 852

BLAST of Cmc08g0227761 vs. ExPASy TrEMBL
Match: A0A7N2N1S1 (Integrase catalytic domain-containing protein OS=Quercus lobata OX=97700 PE=4 SV=1)

HSP 1 Score: 312.0 bits (798), Expect = 2.6e-81
Identity = 162/277 (58.48%), Postives = 198/277 (71.48%), Query Frame = 0

Query: 16  RIKRLHSDRGTEYDSVAFNEFYNSKGIIHKTTASYSTEMNGKAERKNRTLTKLVVAILLE 75
           +IKR+ SDRG EY+S AFN F  S GIIH+TTA YS   NG AERKNRTL +L  A+L+E
Sbjct: 430 KIKRIRSDRGHEYESSAFNSFAQSLGIIHETTAPYSPASNGVAERKNRTLIELTNAMLIE 489

Query: 76  SGAAPSWWGEIIKTVNYVLNRIPKSNSKTSPYEVLKHKTPNVTYLRTWGCLAYVRIPDPK 135
           SGA   +WGE I T  +VLNR+P   S T+P+E+ K   PN+ YLR WGCLAYVR+ DPK
Sbjct: 490 SGAPLHFWGEAILTACHVLNRVPHKKSHTTPFEMWKGHKPNLGYLRVWGCLAYVRLTDPK 549

Query: 136 RRKLVSRVYGCVFIGYTENSKAYRFYDLENKVIIESNDVDFFKDRFPFKSRNSGGLYSQT 195
             KL  R   C F+GY  NS AYRF+DLENK+I ES D  F +++FPFK +NSGG  +  
Sbjct: 550 MPKLGIRATTCAFLGYAINSAAYRFFDLENKIIFESGDAIFHEEKFPFKLKNSGGEENIL 609

Query: 196 SGGSSSSSLPSIRIQTQDKEVDPEPRRSKRARTIKDFGEDFEMYNVED-PKDLTEALSSV 255
           S  SSS+S      Q Q+   + EPRRSKRAR  KDFG D+ ++N+E+ PK+L EAL+S 
Sbjct: 610 SQPSSSTS----HFQNQE-NFEMEPRRSKRARVEKDFGPDYYVFNIEENPKNLKEALTSP 669

Query: 256 DANLWQEAINDEMDSLESNRTWHLVDLPPGCKGIGCK 292
           DA  W+EA+NDEM+SL SNRTW LVDLPPGCK IGCK
Sbjct: 670 DAIFWKEAVNDEMESLISNRTWKLVDLPPGCKTIGCK 701

BLAST of Cmc08g0227761 vs. TAIR 10
Match: ATMG00710.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 43.5 bits (101), Expect = 3.4e-04
Identity = 22/75 (29.33%), Postives = 39/75 (52.00%), Query Frame = 0

Query: 62  NRTLTKLVVAILLESGAAPSWWGEIIKTVNYVLNRIPKSNSKTS-PYEVLKHKTPNVTYL 121
           NRT+ + V ++L E G   ++  +   T  +++N+ P +      P EV     P  +YL
Sbjct: 2   NRTIIEKVRSMLCECGLPKTFRADAANTAVHIINKYPSTAINFHVPDEVWFQSVPTYSYL 61

Query: 122 RTWGCLAYVRIPDPK 136
           R +GC+AY+   + K
Sbjct: 62  RRFGCVAYIHCDEGK 76

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAA0034938.13.1e-14590.18putative Polyprotein [Cucumis melo var. makuwa] >TYK21293.1 putative Polyprotein... [more]
RZC09450.11.6e-10969.62Retrovirus-related Pol polyprotein from transposon TNT 1-94 [Glycine soja][more]
ABI34306.11.3e-7954.95Polyprotein, putative [Solanum demissum][more]
AAU90333.13.0e-7954.95Putative gag and pol polyprotein, identical [Solanum demissum][more]
XP_023158131.22.3e-7149.66uncharacterized protein LOC103653943 isoform X1 [Zea mays] >XP_035823266.1 uncha... [more]
Match NameE-valueIdentityDescription
P109782.0e-3330.51Retrovirus-related Pol polyprotein from transposon TNT 1-94 OS=Nicotiana tabacum... [more]
P041461.5e-2028.11Copia protein OS=Drosophila melanogaster OX=7227 GN=GIP PE=1 SV=3[more]
P0C2J72.0e-0920.48Transposon Ty4-H Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 20... [more]
A0A0B7P3V82.0e-0920.48Transposon Ty4-P Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 20... [more]
P470241.3e-0820.88Transposon Ty4-J Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 20... [more]
Match NameE-valueIdentityDescription
A0A5D3DCJ11.5e-14590.18Putative Polyprotein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold119... [more]
A0A445KFK27.8e-11069.62Retrovirus-related Pol polyprotein from transposon TNT 1-94 OS=Glycine soja OX=3... [more]
A0A7N2L5312.3e-8557.73Uncharacterized protein OS=Quercus lobata OX=97700 PE=4 SV=1[more]
A0A7N2R9F31.6e-8356.70Uncharacterized protein OS=Quercus lobata OX=97700 PE=4 SV=1[more]
A0A7N2N1S12.6e-8158.48Integrase catalytic domain-containing protein OS=Quercus lobata OX=97700 PE=4 SV... [more]
Match NameE-valueIdentityDescription
ATMG00710.13.4e-0429.33Polynucleotidyl transferase, ribonuclease H-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Melon (Charmono) v1.1
Date Performed: 2022-10-13
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR036397Ribonuclease H superfamilyGENE3D3.30.420.10coord: 1..123
e-value: 2.5E-24
score: 87.7
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 189..225
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 189..210
NoneNo IPR availablePANTHERPTHR45895FAMILY NOT NAMEDcoord: 3..201
IPR001584Integrase, catalytic corePROSITEPS50994INTEGRASEcoord: 1..114
score: 15.773809
IPR012337Ribonuclease H-like superfamilySUPERFAMILY53098Ribonuclease H-likecoord: 3..122

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cmc08g0227761.1Cmc08g0227761.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0071897 DNA biosynthetic process
biological_process GO:0015074 DNA integration
biological_process GO:0006468 protein phosphorylation
cellular_component GO:0016020 membrane
molecular_function GO:0003887 DNA-directed DNA polymerase activity
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0000166 nucleotide binding
molecular_function GO:0004672 protein kinase activity
molecular_function GO:0008270 zinc ion binding