Cmc08g0221191 (gene) Melon (Charmono) v1.1

Overview
NameCmc08g0221191
Typegene
OrganismCucumis melo L. var. cantalupensis cv. Charmono (Melon (Charmono) v1.1)
DescriptionProtein NUCLEAR FUSION DEFECTIVE 4
LocationCMiso1.1chr08: 9072076 .. 9074836 (+)
RNA-Seq ExpressionCmc08g0221191
SyntenyCmc08g0221191
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GTTTGATTGATTTTGGTGTAGTTTTATTGTATTTTGTATATGTACTAATACATCAAACCCACATCCAAACCTATAATGAAACGATGAATCTTATTGATTGATAAATAGAGTGTTCAGTCCACCATCATGTCATCCGAATGGATAACGTTTTTTAAGTAATTTATAGATACTTTGATTAACGGAAACAACAAAGGAAGTTTTTTTGCTCCTCTGTTCGCTTTCGCACCTCAACCATGTGAACACAATTTCCACTTCTGTCCAATTGGAACTTAACTAAAATTCAAAGTGTCTTCATCAATCGTCCATGGCCACCATTATAAAATCTTCAATTCAGTTCCCAAGATTCAGCATCGGAACTGGGCAAAGAAGACGATCGCAGATGTCGAATTTCGCCGTCCAAGTTCTATTGGGCCGATGGTTCACCGCCTTCGCTTCTTTACTCATCATGTCCGTTTCCGGTGCCTCCTACATGTTCGCACTTTACTCCTCCGACATCAAATCCTCCTTGAATTACGATCAGACCACTCTCAACCTCGTCGGCTTCTTTAAGGATCTGGGTTCCAACGTTGGAGTCTTTTCCGGTCTTATTAATGAAATTACCCCGCCTTGGGTTGTTCTCTTCATCGGTGGGGTAATGAATTTCTTTGGCTATTTCATGATTTGGCTGTCTGTCACTCACCGTATCTCCAAACCCAAATTGCCCGCGATGTGTTTGTTTATATTCCTAGGTGCAAATTCTCAGACATTTGCCAATACTGGGGCTTTGATACCTTCTGTGAAGAATTTCCCTCAGAATCGAGGTAATGTATTGGGTTTATTGAAAGGGTTTGTTGGGTTAAGTGGGGCAATCTTGACTCAGATTTATCATGCTTTTTATGGGGATGATTCGAAGGATTTTATCTTGCTTATTGCTTGGCTGCCTACTGCTGTTTCTTTACTGCTTCTTCGGATTGTTAGAGTAGTGGAAGTGAACCCAATATTCAAATCCAATGATCTGAAAAATTTCTATTCTATGCTCTACATTTCCCTTGGTCTGGCTGGCTTCCTCATGATTTTGATTATTATCCAAAACGAGCTCATGTTTACTAGAATTCAGTATTTGGTTTGTGTGTTTGTTCTTCTAGCGTTTCTTTTTCTTCCCCTTGTTGTTGTTATAAGAGAAGAATTCAGCATTAGGAAAAGGAAATTGCAAGGTGTGGATGTTACTTCTTGGCTTCCGGTTCCATCAGACCAATCTCCAGCTGAATTACCGTTGCCTAGACCTTCAAGTTTGCGAACGACTGACATAGCTTTAACCAACCCAAGTTCTTGCTTCGAAAACGTGTTCAGACCGCCGGAAAGAGGAGAGGACTACACCATTTTACAAGCAATCTTCAGTGTAGATATGTTGATTCTGTTTTTTACCACAATTTGTGGGGCTGGTGGGACTTTGACTGCAATCGACAACCTGGGTCAGATAGGCAGCTCCCTTGGCTACTCAACTCATACCATTTCTACTTTCACTTCTCTAGTGAGTATATGGGGCTTTCTCGGTCGAGCATTTTCGGGTTATGCTTCTGAATTCTTATGGACGAAGTATAACTTCTCTCGCCCTTTGTTCCTCACTTTTGTCCTTCTTCTCTCTTGCTTTGGCCATCTTCTAATTTCCTCTGGAGTCCCAACCTCCATTTATTTTGCTTCTGTGATCATTGGATTTTGCTTTGGAGCACAGTGGCCATTGATTTTCGCCATTGTTTCTGAATTATTTGGCTTGAAATACTATGCAACTTTATATAGCATTAGTGGAATTGCTAGCCCAATTGGTTCATACATTTTCAATGTGAAAGTAGCTGGCTATTTATACGACCAAGAGGCTAGAAAGCAAATAGGAGCGATCGGTCTTAGAAATGTAGCTGGTAGAGACTTAGCCTGCAAGGGCGTCCATTGTTACAGATTAGCTTTTCTCATTATCTCTGCTTCAACCATGTTCGGTTGTTTTGTTTCATTCATTTTGGTGCTCCGAACTTGGAAATTCTACAAGGGTGATATCTACAAGAAGTTTAGAGATGAAAGAAAGGAATCAAAGTAATCAACTTAGTATTACACTGTTTGTTCAACACACGCCGCAATCTTGTTATCTTACTTCTGTTCCGACATCTGACAGAAAGGTAGAATGTTGCCTTTTATTGTAGCAAAGATGGAGGTTAAGATCTTCATTTAGTGTCTTTTTCTTACCGGTATTTACACTCTTGGTATGATCCAAACACAAAACCGAACTTGTCATGGACAAAACCGAACTTGCCTTTTAATGTGCACGGAGGATCTGGAGTGGTGAATTGCCATTTTGCTCGGAAGCCACAACCTACAGAAACAACTGCTTAGTGCTTACTTTTGTTCAATACGAAGATCGTTCACCTTTCTATCAAAGCTTTCTTCTTTTGCTTATCAGTCAGTTAAAAAGAAAAAGGCTAAAAGATGATACTTGTCTTTTTCGAGTTTCAACAATTGTGAGGATGGAGATCTAACCATCAATTTTTAAGATGGTAATTATTGGCTTTATCGAGCTTGGATTGACTTACGAATAGGCTTTGAAGTTCCTTTTTGTAAGTACATGTACCTTTGTAAATTATCTGTACACAACCACAACATTTATCTAATGAAGTTGATTAATTGTAACATCACATTTATATTGGCTGGTTTTCTTATCTGCTTCTGGAAAGATATGTGTGAATGAGCTGTTCTTGTCAAGCAAAGTTATGGAGACTCTTTTTTCTTTGCATCT

mRNA sequence

GTTTGATTGATTTTGGTGTAGTTTTATTGTATTTTGTATATGTACTAATACATCAAACCCACATCCAAACCTATAATGAAACGATGAATCTTATTGATTGATAAATAGAGTGTTCAGTCCACCATCATGTCATCCGAATGGATAACGTTTTTTAAGTAATTTATAGATACTTTGATTAACGGAAACAACAAAGGAAGTTTTTTTGCTCCTCTGTTCGCTTTCGCACCTCAACCATGTGAACACAATTTCCACTTCTGTCCAATTGGAACTTAACTAAAATTCAAAGTGTCTTCATCAATCGTCCATGGCCACCATTATAAAATCTTCAATTCAGTTCCCAAGATTCAGCATCGGAACTGGGCAAAGAAGACGATCGCAGATGTCGAATTTCGCCGTCCAAGTTCTATTGGGCCGATGGTTCACCGCCTTCGCTTCTTTACTCATCATGTCCGTTTCCGGTGCCTCCTACATGTTCGCACTTTACTCCTCCGACATCAAATCCTCCTTGAATTACGATCAGACCACTCTCAACCTCGTCGGCTTCTTTAAGGATCTGGGTTCCAACGTTGGAGTCTTTTCCGGTCTTATTAATGAAATTACCCCGCCTTGGGTTGTTCTCTTCATCGGTGGGGTAATGAATTTCTTTGGCTATTTCATGATTTGGCTGTCTGTCACTCACCGTATCTCCAAACCCAAATTGCCCGCGATGTGTTTGTTTATATTCCTAGGTGCAAATTCTCAGACATTTGCCAATACTGGGGCTTTGATACCTTCTGTGAAGAATTTCCCTCAGAATCGAGGTAATGTATTGGGTTTATTGAAAGGGTTTGTTGGGTTAAGTGGGGCAATCTTGACTCAGATTTATCATGCTTTTTATGGGGATGATTCGAAGGATTTTATCTTGCTTATTGCTTGGCTGCCTACTGCTGTTTCTTTACTGCTTCTTCGGATTGTTAGAGTAGTGGAAGTGAACCCAATATTCAAATCCAATGATCTGAAAAATTTCTATTCTATGCTCTACATTTCCCTTGGTCTGGCTGGCTTCCTCATGATTTTGATTATTATCCAAAACGAGCTCATGTTTACTAGAATTCAGTATTTGGTTTGTGTGTTTGTTCTTCTAGCGTTTCTTTTTCTTCCCCTTGTTGTTGTTATAAGAGAAGAATTCAGCATTAGGAAAAGGAAATTGCAAGGTGTGGATGTTACTTCTTGGCTTCCGGTTCCATCAGACCAATCTCCAGCTGAATTACCGTTGCCTAGACCTTCAAGTTTGCGAACGACTGACATAGCTTTAACCAACCCAAGTTCTTGCTTCGAAAACGTGTTCAGACCGCCGGAAAGAGGAGAGGACTACACCATTTTACAAGCAATCTTCAGTGTAGATATGTTGATTCTGTTTTTTACCACAATTTGTGGGGCTGGTGGGACTTTGACTGCAATCGACAACCTGGGTCAGATAGGCAGCTCCCTTGGCTACTCAACTCATACCATTTCTACTTTCACTTCTCTAGTGAGTATATGGGGCTTTCTCGGTCGAGCATTTTCGGGTTATGCTTCTGAATTCTTATGGACGAAGTATAACTTCTCTCGCCCTTTGTTCCTCACTTTTGTCCTTCTTCTCTCTTGCTTTGGCCATCTTCTAATTTCCTCTGGAGTCCCAACCTCCATTTATTTTGCTTCTGTGATCATTGGATTTTGCTTTGGAGCACAGTGGCCATTGATTTTCGCCATTGTTTCTGAATTATTTGGCTTGAAATACTATGCAACTTTATATAGCATTAGTGGAATTGCTAGCCCAATTGGTTCATACATTTTCAATGTGAAAGTAGCTGGCTATTTATACGACCAAGAGGCTAGAAAGCAAATAGGAGCGATCGGTCTTAGAAATGTAGCTGGTAGAGACTTAGCCTGCAAGGGCGTCCATTGTTACAGATTAGCTTTTCTCATTATCTCTGCTTCAACCATGTTCGGTTGTTTTGTTTCATTCATTTTGGTGCTCCGAACTTGGAAATTCTACAAGGGTGATATCTACAAGAAGTTTAGAGATGAAAGAAAGGAATCAAAGTAATCAACTTAGTATTACACTGTTTGTTCAACACACGCCGCAATCTTGTTATCTTACTTCTGTTCCGACATCTGACAGAAAGGTAGAATGTTGCCTTTTATTGTAGCAAAGATGGAGGTTAAGATCTTCATTTAGTGTCTTTTTCTTACCGGTATTTACACTCTTGGTATGATCCAAACACAAAACCGAACTTGTCATGGACAAAACCGAACTTGCCTTTTAATGTGCACGGAGGATCTGGAGTGGTGAATTGCCATTTTGCTCGGAAGCCACAACCTACAGAAACAACTGCTTAGTGCTTACTTTTGTTCAATACGAAGATCGTTCACCTTTCTATCAAAGCTTTCTTCTTTTGCTTATCAGTCAGTTAAAAAGAAAAAGGCTAAAAGATGATACTTGTCTTTTTCGAGTTTCAACAATTGTGAGGATGGAGATCTAACCATCAATTTTTAAGATGGTAATTATTGGCTTTATCGAGCTTGGATTGACTTACGAATAGGCTTTGAAGTTCCTTTTTGTAAGTACATGTACCTTTGTAAATTATCTGTACACAACCACAACATTTATCTAATGAAGTTGATTAATTGTAACATCACATTTATATTGGCTGGTTTTCTTATCTGCTTCTGGAAAGATATGTGTGAATGAGCTGTTCTTGTCAAGCAAAGTTATGGAGACTCTTTTTTCTTTGCATCT

Coding sequence (CDS)

ATGGCCACCATTATAAAATCTTCAATTCAGTTCCCAAGATTCAGCATCGGAACTGGGCAAAGAAGACGATCGCAGATGTCGAATTTCGCCGTCCAAGTTCTATTGGGCCGATGGTTCACCGCCTTCGCTTCTTTACTCATCATGTCCGTTTCCGGTGCCTCCTACATGTTCGCACTTTACTCCTCCGACATCAAATCCTCCTTGAATTACGATCAGACCACTCTCAACCTCGTCGGCTTCTTTAAGGATCTGGGTTCCAACGTTGGAGTCTTTTCCGGTCTTATTAATGAAATTACCCCGCCTTGGGTTGTTCTCTTCATCGGTGGGGTAATGAATTTCTTTGGCTATTTCATGATTTGGCTGTCTGTCACTCACCGTATCTCCAAACCCAAATTGCCCGCGATGTGTTTGTTTATATTCCTAGGTGCAAATTCTCAGACATTTGCCAATACTGGGGCTTTGATACCTTCTGTGAAGAATTTCCCTCAGAATCGAGGTAATGTATTGGGTTTATTGAAAGGGTTTGTTGGGTTAAGTGGGGCAATCTTGACTCAGATTTATCATGCTTTTTATGGGGATGATTCGAAGGATTTTATCTTGCTTATTGCTTGGCTGCCTACTGCTGTTTCTTTACTGCTTCTTCGGATTGTTAGAGTAGTGGAAGTGAACCCAATATTCAAATCCAATGATCTGAAAAATTTCTATTCTATGCTCTACATTTCCCTTGGTCTGGCTGGCTTCCTCATGATTTTGATTATTATCCAAAACGAGCTCATGTTTACTAGAATTCAGTATTTGGTTTGTGTGTTTGTTCTTCTAGCGTTTCTTTTTCTTCCCCTTGTTGTTGTTATAAGAGAAGAATTCAGCATTAGGAAAAGGAAATTGCAAGGTGTGGATGTTACTTCTTGGCTTCCGGTTCCATCAGACCAATCTCCAGCTGAATTACCGTTGCCTAGACCTTCAAGTTTGCGAACGACTGACATAGCTTTAACCAACCCAAGTTCTTGCTTCGAAAACGTGTTCAGACCGCCGGAAAGAGGAGAGGACTACACCATTTTACAAGCAATCTTCAGTGTAGATATGTTGATTCTGTTTTTTACCACAATTTGTGGGGCTGGTGGGACTTTGACTGCAATCGACAACCTGGGTCAGATAGGCAGCTCCCTTGGCTACTCAACTCATACCATTTCTACTTTCACTTCTCTAGTGAGTATATGGGGCTTTCTCGGTCGAGCATTTTCGGGTTATGCTTCTGAATTCTTATGGACGAAGTATAACTTCTCTCGCCCTTTGTTCCTCACTTTTGTCCTTCTTCTCTCTTGCTTTGGCCATCTTCTAATTTCCTCTGGAGTCCCAACCTCCATTTATTTTGCTTCTGTGATCATTGGATTTTGCTTTGGAGCACAGTGGCCATTGATTTTCGCCATTGTTTCTGAATTATTTGGCTTGAAATACTATGCAACTTTATATAGCATTAGTGGAATTGCTAGCCCAATTGGTTCATACATTTTCAATGTGAAAGTAGCTGGCTATTTATACGACCAAGAGGCTAGAAAGCAAATAGGAGCGATCGGTCTTAGAAATGTAGCTGGTAGAGACTTAGCCTGCAAGGGCGTCCATTGTTACAGATTAGCTTTTCTCATTATCTCTGCTTCAACCATGTTCGGTTGTTTTGTTTCATTCATTTTGGTGCTCCGAACTTGGAAATTCTACAAGGGTGATATCTACAAGAAGTTTAGAGATGAAAGAAAGGAATCAAAGTAA

Protein sequence

MATIIKSSIQFPRFSIGTGQRRRSQMSNFAVQVLLGRWFTAFASLLIMSVSGASYMFALYSSDIKSSLNYDQTTLNLVGFFKDLGSNVGVFSGLINEITPPWVVLFIGGVMNFFGYFMIWLSVTHRISKPKLPAMCLFIFLGANSQTFANTGALIPSVKNFPQNRGNVLGLLKGFVGLSGAILTQIYHAFYGDDSKDFILLIAWLPTAVSLLLLRIVRVVEVNPIFKSNDLKNFYSMLYISLGLAGFLMILIIIQNELMFTRIQYLVCVFVLLAFLFLPLVVVIREEFSIRKRKLQGVDVTSWLPVPSDQSPAELPLPRPSSLRTTDIALTNPSSCFENVFRPPERGEDYTILQAIFSVDMLILFFTTICGAGGTLTAIDNLGQIGSSLGYSTHTISTFTSLVSIWGFLGRAFSGYASEFLWTKYNFSRPLFLTFVLLLSCFGHLLISSGVPTSIYFASVIIGFCFGAQWPLIFAIVSELFGLKYYATLYSISGIASPIGSYIFNVKVAGYLYDQEARKQIGAIGLRNVAGRDLACKGVHCYRLAFLIISASTMFGCFVSFILVLRTWKFYKGDIYKKFRDERKESK
Homology
BLAST of Cmc08g0221191 vs. NCBI nr
Match: XP_008462497.1 (PREDICTED: uncharacterized protein LOC103500834 [Cucumis melo])

HSP 1 Score: 1138.3 bits (2943), Expect = 0.0e+00
Identity = 587/587 (100.00%), Postives = 587/587 (100.00%), Query Frame = 0

Query: 1   MATIIKSSIQFPRFSIGTGQRRRSQMSNFAVQVLLGRWFTAFASLLIMSVSGASYMFALY 60
           MATIIKSSIQFPRFSIGTGQRRRSQMSNFAVQVLLGRWFTAFASLLIMSVSGASYMFALY
Sbjct: 1   MATIIKSSIQFPRFSIGTGQRRRSQMSNFAVQVLLGRWFTAFASLLIMSVSGASYMFALY 60

Query: 61  SSDIKSSLNYDQTTLNLVGFFKDLGSNVGVFSGLINEITPPWVVLFIGGVMNFFGYFMIW 120
           SSDIKSSLNYDQTTLNLVGFFKDLGSNVGVFSGLINEITPPWVVLFIGGVMNFFGYFMIW
Sbjct: 61  SSDIKSSLNYDQTTLNLVGFFKDLGSNVGVFSGLINEITPPWVVLFIGGVMNFFGYFMIW 120

Query: 121 LSVTHRISKPKLPAMCLFIFLGANSQTFANTGALIPSVKNFPQNRGNVLGLLKGFVGLSG 180
           LSVTHRISKPKLPAMCLFIFLGANSQTFANTGALIPSVKNFPQNRGNVLGLLKGFVGLSG
Sbjct: 121 LSVTHRISKPKLPAMCLFIFLGANSQTFANTGALIPSVKNFPQNRGNVLGLLKGFVGLSG 180

Query: 181 AILTQIYHAFYGDDSKDFILLIAWLPTAVSLLLLRIVRVVEVNPIFKSNDLKNFYSMLYI 240
           AILTQIYHAFYGDDSKDFILLIAWLPTAVSLLLLRIVRVVEVNPIFKSNDLKNFYSMLYI
Sbjct: 181 AILTQIYHAFYGDDSKDFILLIAWLPTAVSLLLLRIVRVVEVNPIFKSNDLKNFYSMLYI 240

Query: 241 SLGLAGFLMILIIIQNELMFTRIQYLVCVFVLLAFLFLPLVVVIREEFSIRKRKLQGVDV 300
           SLGLAGFLMILIIIQNELMFTRIQYLVCVFVLLAFLFLPLVVVIREEFSIRKRKLQGVDV
Sbjct: 241 SLGLAGFLMILIIIQNELMFTRIQYLVCVFVLLAFLFLPLVVVIREEFSIRKRKLQGVDV 300

Query: 301 TSWLPVPSDQSPAELPLPRPSSLRTTDIALTNPSSCFENVFRPPERGEDYTILQAIFSVD 360
           TSWLPVPSDQSPAELPLPRPSSLRTTDIALTNPSSCFENVFRPPERGEDYTILQAIFSVD
Sbjct: 301 TSWLPVPSDQSPAELPLPRPSSLRTTDIALTNPSSCFENVFRPPERGEDYTILQAIFSVD 360

Query: 361 MLILFFTTICGAGGTLTAIDNLGQIGSSLGYSTHTISTFTSLVSIWGFLGRAFSGYASEF 420
           MLILFFTTICGAGGTLTAIDNLGQIGSSLGYSTHTISTFTSLVSIWGFLGRAFSGYASEF
Sbjct: 361 MLILFFTTICGAGGTLTAIDNLGQIGSSLGYSTHTISTFTSLVSIWGFLGRAFSGYASEF 420

Query: 421 LWTKYNFSRPLFLTFVLLLSCFGHLLISSGVPTSIYFASVIIGFCFGAQWPLIFAIVSEL 480
           LWTKYNFSRPLFLTFVLLLSCFGHLLISSGVPTSIYFASVIIGFCFGAQWPLIFAIVSEL
Sbjct: 421 LWTKYNFSRPLFLTFVLLLSCFGHLLISSGVPTSIYFASVIIGFCFGAQWPLIFAIVSEL 480

Query: 481 FGLKYYATLYSISGIASPIGSYIFNVKVAGYLYDQEARKQIGAIGLRNVAGRDLACKGVH 540
           FGLKYYATLYSISGIASPIGSYIFNVKVAGYLYDQEARKQIGAIGLRNVAGRDLACKGVH
Sbjct: 481 FGLKYYATLYSISGIASPIGSYIFNVKVAGYLYDQEARKQIGAIGLRNVAGRDLACKGVH 540

Query: 541 CYRLAFLIISASTMFGCFVSFILVLRTWKFYKGDIYKKFRDERKESK 588
           CYRLAFLIISASTMFGCFVSFILVLRTWKFYKGDIYKKFRDERKESK
Sbjct: 541 CYRLAFLIISASTMFGCFVSFILVLRTWKFYKGDIYKKFRDERKESK 587

BLAST of Cmc08g0221191 vs. NCBI nr
Match: TYK07328.1 (protein NUCLEAR FUSION DEFECTIVE 4 [Cucumis melo var. makuwa])

HSP 1 Score: 1135.2 bits (2935), Expect = 0.0e+00
Identity = 584/587 (99.49%), Postives = 586/587 (99.83%), Query Frame = 0

Query: 1   MATIIKSSIQFPRFSIGTGQRRRSQMSNFAVQVLLGRWFTAFASLLIMSVSGASYMFALY 60
           MATIIKSSIQFPRFSIGTGQRRRSQMSNFAVQVLLGRWFTAFASLLIMSVSGASYMFALY
Sbjct: 1   MATIIKSSIQFPRFSIGTGQRRRSQMSNFAVQVLLGRWFTAFASLLIMSVSGASYMFALY 60

Query: 61  SSDIKSSLNYDQTTLNLVGFFKDLGSNVGVFSGLINEITPPWVVLFIGGVMNFFGYFMIW 120
           SSDIKSSLNYDQTTLNLVGFFKDLGSNVGVFSGLINEITPPWVVLFIGGVMNFFGYFMIW
Sbjct: 61  SSDIKSSLNYDQTTLNLVGFFKDLGSNVGVFSGLINEITPPWVVLFIGGVMNFFGYFMIW 120

Query: 121 LSVTHRISKPKLPAMCLFIFLGANSQTFANTGALIPSVKNFPQNRGNVLGLLKGFVGLSG 180
           LSVTHRISKPKLPAMCLF+FLGANSQTFANTGALIPSVKNFPQNRGNVLGLLKGFVGLSG
Sbjct: 121 LSVTHRISKPKLPAMCLFVFLGANSQTFANTGALIPSVKNFPQNRGNVLGLLKGFVGLSG 180

Query: 181 AILTQIYHAFYGDDSKDFILLIAWLPTAVSLLLLRIVRVVEVNPIFKSNDLKNFYSMLYI 240
           AILTQIYHAFYGDDSKDFILLIAWLPTAVSLLLLRIVRVVEVNPIFKSNDLKNFYSMLYI
Sbjct: 181 AILTQIYHAFYGDDSKDFILLIAWLPTAVSLLLLRIVRVVEVNPIFKSNDLKNFYSMLYI 240

Query: 241 SLGLAGFLMILIIIQNELMFTRIQYLVCVFVLLAFLFLPLVVVIREEFSIRKRKLQGVDV 300
           SLGLAGFLMILIIIQNELMFTRIQYLVCVFVLLAFLFLPLVVVIREEFSIRKRKLQGVDV
Sbjct: 241 SLGLAGFLMILIIIQNELMFTRIQYLVCVFVLLAFLFLPLVVVIREEFSIRKRKLQGVDV 300

Query: 301 TSWLPVPSDQSPAELPLPRPSSLRTTDIALTNPSSCFENVFRPPERGEDYTILQAIFSVD 360
           TSWLPVPSDQSPAELPLPRPSSLRTTDIALTNPSSCFENVFRPPERGEDYTILQAIFSVD
Sbjct: 301 TSWLPVPSDQSPAELPLPRPSSLRTTDIALTNPSSCFENVFRPPERGEDYTILQAIFSVD 360

Query: 361 MLILFFTTICGAGGTLTAIDNLGQIGSSLGYSTHTISTFTSLVSIWGFLGRAFSGYASEF 420
           MLILFFTTICGAGGTLTAIDNLGQIGSSLGYSTHTISTFTSLVSIWGFLGRAFSGYASEF
Sbjct: 361 MLILFFTTICGAGGTLTAIDNLGQIGSSLGYSTHTISTFTSLVSIWGFLGRAFSGYASEF 420

Query: 421 LWTKYNFSRPLFLTFVLLLSCFGHLLISSGVPTSIYFASVIIGFCFGAQWPLIFAIVSEL 480
           LWTKYNFSRPLFLTFVLL SCFGHLLI+SGVPTSIYFASVIIGFCFGAQWPLIFAIVSEL
Sbjct: 421 LWTKYNFSRPLFLTFVLLFSCFGHLLIASGVPTSIYFASVIIGFCFGAQWPLIFAIVSEL 480

Query: 481 FGLKYYATLYSISGIASPIGSYIFNVKVAGYLYDQEARKQIGAIGLRNVAGRDLACKGVH 540
           FGLKYYATLYSISGIASPIGSYIFNVKVAGYLYDQEARKQIGAIGLRNVAGRDLACKGVH
Sbjct: 481 FGLKYYATLYSISGIASPIGSYIFNVKVAGYLYDQEARKQIGAIGLRNVAGRDLACKGVH 540

Query: 541 CYRLAFLIISASTMFGCFVSFILVLRTWKFYKGDIYKKFRDERKESK 588
           CYRLAFLIISASTMFGCFVSFILVLRTWKFYKGDIYKKFRDERKESK
Sbjct: 541 CYRLAFLIISASTMFGCFVSFILVLRTWKFYKGDIYKKFRDERKESK 587

BLAST of Cmc08g0221191 vs. NCBI nr
Match: KAA0063797.1 (protein NUCLEAR FUSION DEFECTIVE 4 [Cucumis melo var. makuwa])

HSP 1 Score: 1133.2 bits (2930), Expect = 0.0e+00
Identity = 584/587 (99.49%), Postives = 585/587 (99.66%), Query Frame = 0

Query: 1   MATIIKSSIQFPRFSIGTGQRRRSQMSNFAVQVLLGRWFTAFASLLIMSVSGASYMFALY 60
           MATIIKSSIQFPRFSIGTGQRRRSQMSNFAVQVLLGRWFTAFASLLIMSVSGASYMFALY
Sbjct: 1   MATIIKSSIQFPRFSIGTGQRRRSQMSNFAVQVLLGRWFTAFASLLIMSVSGASYMFALY 60

Query: 61  SSDIKSSLNYDQTTLNLVGFFKDLGSNVGVFSGLINEITPPWVVLFIGGVMNFFGYFMIW 120
           SSDIKSSLNYDQTTLNLVGFFKDLGSNVGVFSGLINEITPPWVVLFIGGVMNFFGYFMIW
Sbjct: 61  SSDIKSSLNYDQTTLNLVGFFKDLGSNVGVFSGLINEITPPWVVLFIGGVMNFFGYFMIW 120

Query: 121 LSVTHRISKPKLPAMCLFIFLGANSQTFANTGALIPSVKNFPQNRGNVLGLLKGFVGLSG 180
           LSVTHRISKPKLPAMCLFIFLGANSQTFANTGALIPSVKNFPQNRGNVLGLLKGFVGLSG
Sbjct: 121 LSVTHRISKPKLPAMCLFIFLGANSQTFANTGALIPSVKNFPQNRGNVLGLLKGFVGLSG 180

Query: 181 AILTQIYHAFYGDDSKDFILLIAWLPTAVSLLLLRIVRVVEVNPIFKSNDLKNFYSMLYI 240
           AILTQIYHAFYGDDSKDFILLIAWLPTAVSLLLLRIVRVVEVNPIFKSNDLKNFYSMLYI
Sbjct: 181 AILTQIYHAFYGDDSKDFILLIAWLPTAVSLLLLRIVRVVEVNPIFKSNDLKNFYSMLYI 240

Query: 241 SLGLAGFLMILIIIQNELMFTRIQYLVCVFVLLAFLFLPLVVVIREEFSIRKRKLQGVDV 300
           SLGLAGFLMILIIIQNELMFTRIQYLVCVFVLLAFLFLPLVVVIREEFSIRKRKLQGVDV
Sbjct: 241 SLGLAGFLMILIIIQNELMFTRIQYLVCVFVLLAFLFLPLVVVIREEFSIRKRKLQGVDV 300

Query: 301 TSWLPVPSDQSPAELPLPRPSSLRTTDIALTNPSSCFENVFRPPERGEDYTILQAIFSVD 360
           TSWLPVPSDQSPAELPLPRPSSLR TDIALTNPSSCFENVFRPPERGEDYTILQAIFSVD
Sbjct: 301 TSWLPVPSDQSPAELPLPRPSSLRMTDIALTNPSSCFENVFRPPERGEDYTILQAIFSVD 360

Query: 361 MLILFFTTICGAGGTLTAIDNLGQIGSSLGYSTHTISTFTSLVSIWGFLGRAFSGYASEF 420
           MLILFFTTICGAGGTLTAIDNLGQIGSSLGYSTHTISTFTSLVSIWGFLGRAFSGYASEF
Sbjct: 361 MLILFFTTICGAGGTLTAIDNLGQIGSSLGYSTHTISTFTSLVSIWGFLGRAFSGYASEF 420

Query: 421 LWTKYNFSRPLFLTFVLLLSCFGHLLISSGVPTSIYFASVIIGFCFGAQWPLIFAIVSEL 480
           LWTKYNFSRPLFLTFVLL SCFGHLLI+SGVPTSIYFASVIIGFCFGAQWPLIFAIVSEL
Sbjct: 421 LWTKYNFSRPLFLTFVLLFSCFGHLLIASGVPTSIYFASVIIGFCFGAQWPLIFAIVSEL 480

Query: 481 FGLKYYATLYSISGIASPIGSYIFNVKVAGYLYDQEARKQIGAIGLRNVAGRDLACKGVH 540
           FGLKYYATLYSISGIASPIGSYIFNVKVAGYLYDQEARKQIGAIGLRNVAGRDLACKGVH
Sbjct: 481 FGLKYYATLYSISGIASPIGSYIFNVKVAGYLYDQEARKQIGAIGLRNVAGRDLACKGVH 540

Query: 541 CYRLAFLIISASTMFGCFVSFILVLRTWKFYKGDIYKKFRDERKESK 588
           CYRLAFLIISASTMFGCFVSFILVLRTWKFYKGDIYKKFRDERKESK
Sbjct: 541 CYRLAFLIISASTMFGCFVSFILVLRTWKFYKGDIYKKFRDERKESK 587

BLAST of Cmc08g0221191 vs. NCBI nr
Match: XP_004143363.2 (uncharacterized protein LOC101203981 [Cucumis sativus] >KAE8647411.1 hypothetical protein Csa_004435 [Cucumis sativus])

HSP 1 Score: 1070.8 bits (2768), Expect = 4.1e-309
Identity = 551/587 (93.87%), Postives = 563/587 (95.91%), Query Frame = 0

Query: 1   MATIIKSSIQFPRFSIGTGQRRRSQMSNFAVQVLLGRWFTAFASLLIMSVSGASYMFALY 60
           MATI+KS IQFP FSI TGQRRRSQMSNFAVQVLLGRWFT FASLLIMSVSGA+YMFALY
Sbjct: 1   MATILKSLIQFPTFSIRTGQRRRSQMSNFAVQVLLGRWFTVFASLLIMSVSGATYMFALY 60

Query: 61  SSDIKSSLNYDQTTLNLVGFFKDLGSNVGVFSGLINEITPPWVVLFIGGVMNFFGYFMIW 120
           SSDIKSSLNYDQTTLNLVGFFKDLGSNVGVFSGLINEITPPWVVLFIGGVMNFFGYFMIW
Sbjct: 61  SSDIKSSLNYDQTTLNLVGFFKDLGSNVGVFSGLINEITPPWVVLFIGGVMNFFGYFMIW 120

Query: 121 LSVTHRISKPKLPAMCLFIFLGANSQTFANTGALIPSVKNFPQNRGNVLGLLKGFVGLSG 180
           LSVTHRI KPKLPAMCLF FLGANSQTFANTGALIPSVKNFPQNRG VLGLLKGFVGLSG
Sbjct: 121 LSVTHRIPKPKLPAMCLFTFLGANSQTFANTGALIPSVKNFPQNRGYVLGLLKGFVGLSG 180

Query: 181 AILTQIYHAFYGDDSKDFILLIAWLPTAVSLLLLRIVRVVEVNPIFKSNDLKNFYSMLYI 240
           AILTQIYHAFYGDDSKDFILLIAWLPTAVSLLLLRIVRVVE NP FKSNDLKNFYSMLYI
Sbjct: 181 AILTQIYHAFYGDDSKDFILLIAWLPTAVSLLLLRIVRVVEANPTFKSNDLKNFYSMLYI 240

Query: 241 SLGLAGFLMILIIIQNELMFTRIQYLVCVFVLLAFLFLPLVVVIREEFSIRKRKLQGVDV 300
           SLGLAGFLMILIIIQNELMFTRIQYL CVFVLL FLFLPLVV+IREEF IRKRKLQGVDV
Sbjct: 241 SLGLAGFLMILIIIQNELMFTRIQYLGCVFVLLTFLFLPLVVIIREEFGIRKRKLQGVDV 300

Query: 301 TSWLPVPSDQSPAELPLPRPSSLRTTDIALTNPSSCFENVFRPPERGEDYTILQAIFSVD 360
           TSWLPVPSD+SP ELPLPR SS  TTD AL NPSSCFENVFRPPERGEDYTILQAIFSVD
Sbjct: 301 TSWLPVPSDESPDELPLPRTSSFPTTDTALANPSSCFENVFRPPERGEDYTILQAIFSVD 360

Query: 361 MLILFFTTICGAGGTLTAIDNLGQIGSSLGYSTHTISTFTSLVSIWGFLGRAFSGYASEF 420
           MLILFF TICGAGGTLTA+DNLGQIGSSLGYSTHTISTFTSLVSIWGFLGRAFSGYASEF
Sbjct: 361 MLILFFVTICGAGGTLTAMDNLGQIGSSLGYSTHTISTFTSLVSIWGFLGRAFSGYASEF 420

Query: 421 LWTKYNFSRPLFLTFVLLLSCFGHLLISSGVPTSIYFASVIIGFCFGAQWPLIFAIVSEL 480
           LWTKYNFSRPLFLT VLLLSCFGHLLI+SG+PTS+YFASVIIGFCFGAQWPLIFAIVSEL
Sbjct: 421 LWTKYNFSRPLFLTLVLLLSCFGHLLIASGLPTSVYFASVIIGFCFGAQWPLIFAIVSEL 480

Query: 481 FGLKYYATLYSISGIASPIGSYIFNVKVAGYLYDQEARKQIGAIGLRNVAGRDLACKGVH 540
           FGLKYYATLYSISGIASP+GSYIFNVKVAGYLYDQEARKQ+   GLRNVAGRDLACKGVH
Sbjct: 481 FGLKYYATLYSISGIASPVGSYIFNVKVAGYLYDQEARKQMD-FGLRNVAGRDLACKGVH 540

Query: 541 CYRLAFLIISASTMFGCFVSFILVLRTWKFYKGDIYKKFRDERKESK 588
           CYRLAFLIISA+TMFGCFVSFILVLRTWKFYK DIYKKFRDERKES+
Sbjct: 541 CYRLAFLIISAATMFGCFVSFILVLRTWKFYKDDIYKKFRDERKESR 586

BLAST of Cmc08g0221191 vs. NCBI nr
Match: XP_038882767.1 (protein NUCLEAR FUSION DEFECTIVE 4-like [Benincasa hispida])

HSP 1 Score: 1013.8 bits (2620), Expect = 5.8e-292
Identity = 523/575 (90.96%), Postives = 543/575 (94.43%), Query Frame = 0

Query: 13  RFSIGTGQRRRSQMSNFAVQVLLGRWFTAFASLLIMSVSGASYMFALYSSDIKSSLNYDQ 72
           RFSI TG+RR S M+NF VQVLLGRWFT FASLLIMSVSGA+YMFALYS+DIKSSLNYDQ
Sbjct: 5   RFSIRTGRRRLSPMTNFVVQVLLGRWFTVFASLLIMSVSGATYMFALYSADIKSSLNYDQ 64

Query: 73  TTLNLVGFFKDLGSNVGVFSGLINEITPPWVVLFIGGVMNFFGYFMIWLSVTHRISKPKL 132
           TTLNL+GFFKDLGSNVGVFSGLI EITPPWVVLFIGGVMNFFGYFMIWLSVTHRI KPKL
Sbjct: 65  TTLNLIGFFKDLGSNVGVFSGLITEITPPWVVLFIGGVMNFFGYFMIWLSVTHRIPKPKL 124

Query: 133 PAMCLFIFLGANSQTFANTGALIPSVKNFPQNRGNVLGLLKGFVGLSGAILTQIYHAFYG 192
           PAMCLFI LGANSQTFANTGALIPSVKNFPQNRGNVLGLLKGFVGLSGAILTQIYHAFYG
Sbjct: 125 PAMCLFICLGANSQTFANTGALIPSVKNFPQNRGNVLGLLKGFVGLSGAILTQIYHAFYG 184

Query: 193 DDSKDFILLIAWLPTAVSLLLLRIVRVVEVNPIFKSNDLKNFYSMLYISLGLAGFLMILI 252
           DDSKDFILLIAWLPTAVS LLLRIVRVVEVNPIFKSNDLKNFYSMLYISLGLAGFLMILI
Sbjct: 185 DDSKDFILLIAWLPTAVSFLLLRIVRVVEVNPIFKSNDLKNFYSMLYISLGLAGFLMILI 244

Query: 253 IIQNELMFTRIQYLVCVFVLLAFLFLPLVVVIREEFSIRKRKLQGVDVTSWLPVPSDQSP 312
           IIQNELMFTRIQYL C FVLLAFLFLPLVVVIREEFS RKRKLQGVD+TSWLPVP D SP
Sbjct: 245 IIQNELMFTRIQYLGCAFVLLAFLFLPLVVVIREEFSSRKRKLQGVDLTSWLPVPPDPSP 304

Query: 313 AELPLPRPSSLRTTDIALTNPSSCFENVFRPPERGEDYTILQAIFSVDMLILFFTTICGA 372
           AELPLPRPS+         + SSCFENVFRPPERGEDYTILQAIFSVDMLILF  TICGA
Sbjct: 305 AELPLPRPSN---------SSSSCFENVFRPPERGEDYTILQAIFSVDMLILFVATICGA 364

Query: 373 GGTLTAIDNLGQIGSSLGYSTHTISTFTSLVSIWGFLGRAFSGYASEFLWTKYNFSRPLF 432
           GGTLTA+DNLGQIGSSLGYSTHTISTFTSLVSIWGFLGRAFSGYASEFLWTKYNFSRPLF
Sbjct: 365 GGTLTAMDNLGQIGSSLGYSTHTISTFTSLVSIWGFLGRAFSGYASEFLWTKYNFSRPLF 424

Query: 433 LTFVLLLSCFGHLLISSGVPTSIYFASVIIGFCFGAQWPLIFAIVSELFGLKYYATLYSI 492
           LT +LLLSC GHLLIS GVPTS+YFASVIIGFCFGAQWPLIFAIVSELFGLKYYATLYSI
Sbjct: 425 LTLLLLLSCVGHLLISFGVPTSLYFASVIIGFCFGAQWPLIFAIVSELFGLKYYATLYSI 484

Query: 493 SGIASPIGSYIFNVKVAGYLYDQEARKQIGAIGLRNVAGRDLACKGVHCYRLAFLIISAS 552
           SGIASP+GSYIFNVKVAGYLYDQEARKQ+ AIGL++VAGRDLAC GVHCYRLAFLIISA+
Sbjct: 485 SGIASPVGSYIFNVKVAGYLYDQEARKQMAAIGLKSVAGRDLACVGVHCYRLAFLIISAA 544

Query: 553 TMFGCFVSFILVLRTWKFYKGDIYKKFRDERKESK 588
           T+FGC VSFILVLRTWKFYKGDIYKKFR+ERKE++
Sbjct: 545 TVFGCVVSFILVLRTWKFYKGDIYKKFREERKEAE 570

BLAST of Cmc08g0221191 vs. ExPASy Swiss-Prot
Match: Q96TW9 (Probable transporter MCH1 OS=Wickerhamomyces anomalus OX=4927 GN=MCH1 PE=3 SV=1)

HSP 1 Score: 49.3 bits (116), Expect = 1.7e-04
Identity = 43/164 (26.22%), Postives = 79/164 (48.17%), Query Frame = 0

Query: 353 LQAIFSVDMLILFFTTICGAGGTLTAIDNLGQIGSSLGYSTHTISTFTSLVSIWGFLGRA 412
           L+ I  +   +L F+ +   G +   I N+G +  ++  ++  IS   ++ +++  L R 
Sbjct: 256 LKFIKDISTYVLLFSLLLSIGPSEMYITNMGSLVKAITPNS-LISDQVAIHAVFSTLSRL 315

Query: 413 FSGYASEFLWTKYNFSRPLFLTFVLLLSCFGHLLI--SSGVPTSIYFASVIIGFCFGAQW 472
             G  S+FL T Y  SR   L  +++L  F  + I  S+ V    Y  S + GF +G  +
Sbjct: 316 SLGALSDFLVTNYQISRSWLLLSIIVLGFFTQIFIATSTFVKDQYYIISALSGFSYGGLF 375

Query: 473 PLIFAIVSELFGLKYYATLYSISGIASPIGSYIFNVKVAGYLYD 515
            L   ++  ++G + + + +    IA  IGS  F + V G +YD
Sbjct: 376 TLYPTVIFSIWGPEIFGSAWGSFMIAPAIGSTTFGM-VFGLVYD 417

BLAST of Cmc08g0221191 vs. ExPASy TrEMBL
Match: A0A1S3CH39 (uncharacterized protein LOC103500834 OS=Cucumis melo OX=3656 GN=LOC103500834 PE=4 SV=1)

HSP 1 Score: 1138.3 bits (2943), Expect = 0.0e+00
Identity = 587/587 (100.00%), Postives = 587/587 (100.00%), Query Frame = 0

Query: 1   MATIIKSSIQFPRFSIGTGQRRRSQMSNFAVQVLLGRWFTAFASLLIMSVSGASYMFALY 60
           MATIIKSSIQFPRFSIGTGQRRRSQMSNFAVQVLLGRWFTAFASLLIMSVSGASYMFALY
Sbjct: 1   MATIIKSSIQFPRFSIGTGQRRRSQMSNFAVQVLLGRWFTAFASLLIMSVSGASYMFALY 60

Query: 61  SSDIKSSLNYDQTTLNLVGFFKDLGSNVGVFSGLINEITPPWVVLFIGGVMNFFGYFMIW 120
           SSDIKSSLNYDQTTLNLVGFFKDLGSNVGVFSGLINEITPPWVVLFIGGVMNFFGYFMIW
Sbjct: 61  SSDIKSSLNYDQTTLNLVGFFKDLGSNVGVFSGLINEITPPWVVLFIGGVMNFFGYFMIW 120

Query: 121 LSVTHRISKPKLPAMCLFIFLGANSQTFANTGALIPSVKNFPQNRGNVLGLLKGFVGLSG 180
           LSVTHRISKPKLPAMCLFIFLGANSQTFANTGALIPSVKNFPQNRGNVLGLLKGFVGLSG
Sbjct: 121 LSVTHRISKPKLPAMCLFIFLGANSQTFANTGALIPSVKNFPQNRGNVLGLLKGFVGLSG 180

Query: 181 AILTQIYHAFYGDDSKDFILLIAWLPTAVSLLLLRIVRVVEVNPIFKSNDLKNFYSMLYI 240
           AILTQIYHAFYGDDSKDFILLIAWLPTAVSLLLLRIVRVVEVNPIFKSNDLKNFYSMLYI
Sbjct: 181 AILTQIYHAFYGDDSKDFILLIAWLPTAVSLLLLRIVRVVEVNPIFKSNDLKNFYSMLYI 240

Query: 241 SLGLAGFLMILIIIQNELMFTRIQYLVCVFVLLAFLFLPLVVVIREEFSIRKRKLQGVDV 300
           SLGLAGFLMILIIIQNELMFTRIQYLVCVFVLLAFLFLPLVVVIREEFSIRKRKLQGVDV
Sbjct: 241 SLGLAGFLMILIIIQNELMFTRIQYLVCVFVLLAFLFLPLVVVIREEFSIRKRKLQGVDV 300

Query: 301 TSWLPVPSDQSPAELPLPRPSSLRTTDIALTNPSSCFENVFRPPERGEDYTILQAIFSVD 360
           TSWLPVPSDQSPAELPLPRPSSLRTTDIALTNPSSCFENVFRPPERGEDYTILQAIFSVD
Sbjct: 301 TSWLPVPSDQSPAELPLPRPSSLRTTDIALTNPSSCFENVFRPPERGEDYTILQAIFSVD 360

Query: 361 MLILFFTTICGAGGTLTAIDNLGQIGSSLGYSTHTISTFTSLVSIWGFLGRAFSGYASEF 420
           MLILFFTTICGAGGTLTAIDNLGQIGSSLGYSTHTISTFTSLVSIWGFLGRAFSGYASEF
Sbjct: 361 MLILFFTTICGAGGTLTAIDNLGQIGSSLGYSTHTISTFTSLVSIWGFLGRAFSGYASEF 420

Query: 421 LWTKYNFSRPLFLTFVLLLSCFGHLLISSGVPTSIYFASVIIGFCFGAQWPLIFAIVSEL 480
           LWTKYNFSRPLFLTFVLLLSCFGHLLISSGVPTSIYFASVIIGFCFGAQWPLIFAIVSEL
Sbjct: 421 LWTKYNFSRPLFLTFVLLLSCFGHLLISSGVPTSIYFASVIIGFCFGAQWPLIFAIVSEL 480

Query: 481 FGLKYYATLYSISGIASPIGSYIFNVKVAGYLYDQEARKQIGAIGLRNVAGRDLACKGVH 540
           FGLKYYATLYSISGIASPIGSYIFNVKVAGYLYDQEARKQIGAIGLRNVAGRDLACKGVH
Sbjct: 481 FGLKYYATLYSISGIASPIGSYIFNVKVAGYLYDQEARKQIGAIGLRNVAGRDLACKGVH 540

Query: 541 CYRLAFLIISASTMFGCFVSFILVLRTWKFYKGDIYKKFRDERKESK 588
           CYRLAFLIISASTMFGCFVSFILVLRTWKFYKGDIYKKFRDERKESK
Sbjct: 541 CYRLAFLIISASTMFGCFVSFILVLRTWKFYKGDIYKKFRDERKESK 587

BLAST of Cmc08g0221191 vs. ExPASy TrEMBL
Match: A0A5D3C849 (Protein NUCLEAR FUSION DEFECTIVE 4 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold202G00430 PE=4 SV=1)

HSP 1 Score: 1135.2 bits (2935), Expect = 0.0e+00
Identity = 584/587 (99.49%), Postives = 586/587 (99.83%), Query Frame = 0

Query: 1   MATIIKSSIQFPRFSIGTGQRRRSQMSNFAVQVLLGRWFTAFASLLIMSVSGASYMFALY 60
           MATIIKSSIQFPRFSIGTGQRRRSQMSNFAVQVLLGRWFTAFASLLIMSVSGASYMFALY
Sbjct: 1   MATIIKSSIQFPRFSIGTGQRRRSQMSNFAVQVLLGRWFTAFASLLIMSVSGASYMFALY 60

Query: 61  SSDIKSSLNYDQTTLNLVGFFKDLGSNVGVFSGLINEITPPWVVLFIGGVMNFFGYFMIW 120
           SSDIKSSLNYDQTTLNLVGFFKDLGSNVGVFSGLINEITPPWVVLFIGGVMNFFGYFMIW
Sbjct: 61  SSDIKSSLNYDQTTLNLVGFFKDLGSNVGVFSGLINEITPPWVVLFIGGVMNFFGYFMIW 120

Query: 121 LSVTHRISKPKLPAMCLFIFLGANSQTFANTGALIPSVKNFPQNRGNVLGLLKGFVGLSG 180
           LSVTHRISKPKLPAMCLF+FLGANSQTFANTGALIPSVKNFPQNRGNVLGLLKGFVGLSG
Sbjct: 121 LSVTHRISKPKLPAMCLFVFLGANSQTFANTGALIPSVKNFPQNRGNVLGLLKGFVGLSG 180

Query: 181 AILTQIYHAFYGDDSKDFILLIAWLPTAVSLLLLRIVRVVEVNPIFKSNDLKNFYSMLYI 240
           AILTQIYHAFYGDDSKDFILLIAWLPTAVSLLLLRIVRVVEVNPIFKSNDLKNFYSMLYI
Sbjct: 181 AILTQIYHAFYGDDSKDFILLIAWLPTAVSLLLLRIVRVVEVNPIFKSNDLKNFYSMLYI 240

Query: 241 SLGLAGFLMILIIIQNELMFTRIQYLVCVFVLLAFLFLPLVVVIREEFSIRKRKLQGVDV 300
           SLGLAGFLMILIIIQNELMFTRIQYLVCVFVLLAFLFLPLVVVIREEFSIRKRKLQGVDV
Sbjct: 241 SLGLAGFLMILIIIQNELMFTRIQYLVCVFVLLAFLFLPLVVVIREEFSIRKRKLQGVDV 300

Query: 301 TSWLPVPSDQSPAELPLPRPSSLRTTDIALTNPSSCFENVFRPPERGEDYTILQAIFSVD 360
           TSWLPVPSDQSPAELPLPRPSSLRTTDIALTNPSSCFENVFRPPERGEDYTILQAIFSVD
Sbjct: 301 TSWLPVPSDQSPAELPLPRPSSLRTTDIALTNPSSCFENVFRPPERGEDYTILQAIFSVD 360

Query: 361 MLILFFTTICGAGGTLTAIDNLGQIGSSLGYSTHTISTFTSLVSIWGFLGRAFSGYASEF 420
           MLILFFTTICGAGGTLTAIDNLGQIGSSLGYSTHTISTFTSLVSIWGFLGRAFSGYASEF
Sbjct: 361 MLILFFTTICGAGGTLTAIDNLGQIGSSLGYSTHTISTFTSLVSIWGFLGRAFSGYASEF 420

Query: 421 LWTKYNFSRPLFLTFVLLLSCFGHLLISSGVPTSIYFASVIIGFCFGAQWPLIFAIVSEL 480
           LWTKYNFSRPLFLTFVLL SCFGHLLI+SGVPTSIYFASVIIGFCFGAQWPLIFAIVSEL
Sbjct: 421 LWTKYNFSRPLFLTFVLLFSCFGHLLIASGVPTSIYFASVIIGFCFGAQWPLIFAIVSEL 480

Query: 481 FGLKYYATLYSISGIASPIGSYIFNVKVAGYLYDQEARKQIGAIGLRNVAGRDLACKGVH 540
           FGLKYYATLYSISGIASPIGSYIFNVKVAGYLYDQEARKQIGAIGLRNVAGRDLACKGVH
Sbjct: 481 FGLKYYATLYSISGIASPIGSYIFNVKVAGYLYDQEARKQIGAIGLRNVAGRDLACKGVH 540

Query: 541 CYRLAFLIISASTMFGCFVSFILVLRTWKFYKGDIYKKFRDERKESK 588
           CYRLAFLIISASTMFGCFVSFILVLRTWKFYKGDIYKKFRDERKESK
Sbjct: 541 CYRLAFLIISASTMFGCFVSFILVLRTWKFYKGDIYKKFRDERKESK 587

BLAST of Cmc08g0221191 vs. ExPASy TrEMBL
Match: A0A5A7V9Y5 (Protein NUCLEAR FUSION DEFECTIVE 4 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold827G00110 PE=4 SV=1)

HSP 1 Score: 1133.2 bits (2930), Expect = 0.0e+00
Identity = 584/587 (99.49%), Postives = 585/587 (99.66%), Query Frame = 0

Query: 1   MATIIKSSIQFPRFSIGTGQRRRSQMSNFAVQVLLGRWFTAFASLLIMSVSGASYMFALY 60
           MATIIKSSIQFPRFSIGTGQRRRSQMSNFAVQVLLGRWFTAFASLLIMSVSGASYMFALY
Sbjct: 1   MATIIKSSIQFPRFSIGTGQRRRSQMSNFAVQVLLGRWFTAFASLLIMSVSGASYMFALY 60

Query: 61  SSDIKSSLNYDQTTLNLVGFFKDLGSNVGVFSGLINEITPPWVVLFIGGVMNFFGYFMIW 120
           SSDIKSSLNYDQTTLNLVGFFKDLGSNVGVFSGLINEITPPWVVLFIGGVMNFFGYFMIW
Sbjct: 61  SSDIKSSLNYDQTTLNLVGFFKDLGSNVGVFSGLINEITPPWVVLFIGGVMNFFGYFMIW 120

Query: 121 LSVTHRISKPKLPAMCLFIFLGANSQTFANTGALIPSVKNFPQNRGNVLGLLKGFVGLSG 180
           LSVTHRISKPKLPAMCLFIFLGANSQTFANTGALIPSVKNFPQNRGNVLGLLKGFVGLSG
Sbjct: 121 LSVTHRISKPKLPAMCLFIFLGANSQTFANTGALIPSVKNFPQNRGNVLGLLKGFVGLSG 180

Query: 181 AILTQIYHAFYGDDSKDFILLIAWLPTAVSLLLLRIVRVVEVNPIFKSNDLKNFYSMLYI 240
           AILTQIYHAFYGDDSKDFILLIAWLPTAVSLLLLRIVRVVEVNPIFKSNDLKNFYSMLYI
Sbjct: 181 AILTQIYHAFYGDDSKDFILLIAWLPTAVSLLLLRIVRVVEVNPIFKSNDLKNFYSMLYI 240

Query: 241 SLGLAGFLMILIIIQNELMFTRIQYLVCVFVLLAFLFLPLVVVIREEFSIRKRKLQGVDV 300
           SLGLAGFLMILIIIQNELMFTRIQYLVCVFVLLAFLFLPLVVVIREEFSIRKRKLQGVDV
Sbjct: 241 SLGLAGFLMILIIIQNELMFTRIQYLVCVFVLLAFLFLPLVVVIREEFSIRKRKLQGVDV 300

Query: 301 TSWLPVPSDQSPAELPLPRPSSLRTTDIALTNPSSCFENVFRPPERGEDYTILQAIFSVD 360
           TSWLPVPSDQSPAELPLPRPSSLR TDIALTNPSSCFENVFRPPERGEDYTILQAIFSVD
Sbjct: 301 TSWLPVPSDQSPAELPLPRPSSLRMTDIALTNPSSCFENVFRPPERGEDYTILQAIFSVD 360

Query: 361 MLILFFTTICGAGGTLTAIDNLGQIGSSLGYSTHTISTFTSLVSIWGFLGRAFSGYASEF 420
           MLILFFTTICGAGGTLTAIDNLGQIGSSLGYSTHTISTFTSLVSIWGFLGRAFSGYASEF
Sbjct: 361 MLILFFTTICGAGGTLTAIDNLGQIGSSLGYSTHTISTFTSLVSIWGFLGRAFSGYASEF 420

Query: 421 LWTKYNFSRPLFLTFVLLLSCFGHLLISSGVPTSIYFASVIIGFCFGAQWPLIFAIVSEL 480
           LWTKYNFSRPLFLTFVLL SCFGHLLI+SGVPTSIYFASVIIGFCFGAQWPLIFAIVSEL
Sbjct: 421 LWTKYNFSRPLFLTFVLLFSCFGHLLIASGVPTSIYFASVIIGFCFGAQWPLIFAIVSEL 480

Query: 481 FGLKYYATLYSISGIASPIGSYIFNVKVAGYLYDQEARKQIGAIGLRNVAGRDLACKGVH 540
           FGLKYYATLYSISGIASPIGSYIFNVKVAGYLYDQEARKQIGAIGLRNVAGRDLACKGVH
Sbjct: 481 FGLKYYATLYSISGIASPIGSYIFNVKVAGYLYDQEARKQIGAIGLRNVAGRDLACKGVH 540

Query: 541 CYRLAFLIISASTMFGCFVSFILVLRTWKFYKGDIYKKFRDERKESK 588
           CYRLAFLIISASTMFGCFVSFILVLRTWKFYKGDIYKKFRDERKESK
Sbjct: 541 CYRLAFLIISASTMFGCFVSFILVLRTWKFYKGDIYKKFRDERKESK 587

BLAST of Cmc08g0221191 vs. ExPASy TrEMBL
Match: A0A6J1CR80 (protein NUCLEAR FUSION DEFECTIVE 4-like OS=Momordica charantia OX=3673 GN=LOC111013589 PE=4 SV=1)

HSP 1 Score: 932.9 bits (2410), Expect = 6.3e-268
Identity = 484/575 (84.17%), Postives = 522/575 (90.78%), Query Frame = 0

Query: 13  RFSIGTGQRRRSQMSNFAVQVLLGRWFTAFASLLIMSVSGASYMFALYSSDIKSSLNYDQ 72
           R  I +G  RR QM NFA+QVLLGRWFT FASLLIM+VSGA+YMF LYS+DIKSSL YDQ
Sbjct: 5   RLRIWSGSGRR-QMRNFALQVLLGRWFTVFASLLIMAVSGATYMFGLYSADIKSSLGYDQ 64

Query: 73  TTLNLVGFFKDLGSNVGVFSGLINEITPPWVVLFIGGVMNFFGYFMIWLSVTHRISKPKL 132
           TTLNL+GFFKDLG+NVGV SGLINEITPPWVVLFIGGVMNFFGYFMIWLSVT RI+KPK+
Sbjct: 65  TTLNLLGFFKDLGANVGVLSGLINEITPPWVVLFIGGVMNFFGYFMIWLSVTQRIAKPKV 124

Query: 133 PAMCLFIFLGANSQTFANTGALIPSVKNFPQNRGNVLGLLKGFVGLSGAILTQIYHAFYG 192
           PAMCL+I LGANSQT+ANTGALIPSVKNFP+ RGNVLGLLKGFVGLSGAILTQ+YHAFYG
Sbjct: 125 PAMCLYICLGANSQTYANTGALIPSVKNFPEYRGNVLGLLKGFVGLSGAILTQLYHAFYG 184

Query: 193 DDSKDFILLIAWLPTAVSLLLLRIVRVVEVNPIFKSNDLKNFYSMLYISLGLAGFLMILI 252
           DDSKDFILLIAWLPTAVS LLLRIVRVVEV+   KSNDLK FY +LYISLGLA FLMILI
Sbjct: 185 DDSKDFILLIAWLPTAVSFLLLRIVRVVEVDQRSKSNDLKIFYHLLYISLGLACFLMILI 244

Query: 253 IIQNELMFTRIQYLVCVFVLLAFLFLPLVVVIREEFSIRKRKLQGVDVTSWLPVPSDQSP 312
           IIQNEL+FTRIQYL C  +LL  LFLPL VVI EEFSI+KRKLQ ++VTSWL   SD SP
Sbjct: 245 IIQNELVFTRIQYLGCALILLVLLFLPLAVVITEEFSIKKRKLQALNVTSWL---SDPSP 304

Query: 313 AELPLPRPSSLRTTDIALTNPSSCFENVFRPPERGEDYTILQAIFSVDMLILFFTTICGA 372
           AELP  RP  L T  +A+ NPSSCFENVFRPPERGEDYTILQAIFS+DMLILF  TICG 
Sbjct: 305 AELPSSRPPHLPTAVVAVANPSSCFENVFRPPERGEDYTILQAIFSIDMLILFVATICGT 364

Query: 373 GGTLTAIDNLGQIGSSLGYSTHTISTFTSLVSIWGFLGRAFSGYASEFLWTKYNFSRPLF 432
           GGTLTAIDNLGQIGSSLGYSTH+ISTFTSLVSIWGFLGRAFSGYASEFLWTKYNFSRPLF
Sbjct: 365 GGTLTAIDNLGQIGSSLGYSTHSISTFTSLVSIWGFLGRAFSGYASEFLWTKYNFSRPLF 424

Query: 433 LTFVLLLSCFGHLLISSGVPTSIYFASVIIGFCFGAQWPLIFAIVSELFGLKYYATLYSI 492
           LT VLLLSC GHLLI+ GVPTS+YFASVIIGFCFGAQWPLIFAIVSELFGLKYYATLYSI
Sbjct: 425 LTLVLLLSCIGHLLIAFGVPTSLYFASVIIGFCFGAQWPLIFAIVSELFGLKYYATLYSI 484

Query: 493 SGIASPIGSYIFNVKVAGYLYDQEARKQIGAIGLRNVAGRDLACKGVHCYRLAFLIISAS 552
           SGIASP+G+YIF+VKVAGYLYD+EAR+Q+ AIGLR VAG DLACKGV CYRLAFLIISA+
Sbjct: 485 SGIASPVGTYIFSVKVAGYLYDEEARRQMAAIGLRRVAG-DLACKGVQCYRLAFLIISAA 544

Query: 553 TMFGCFVSFILVLRTWKFYKGDIYKKFRDERKESK 588
           T+FGC VSFILVLRTW+FYKGDIYKKFR+E KE++
Sbjct: 545 TVFGCIVSFILVLRTWEFYKGDIYKKFREETKEAE 574

BLAST of Cmc08g0221191 vs. ExPASy TrEMBL
Match: A0A6J1ECZ3 (protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucurbita moschata OX=3662 GN=LOC111433047 PE=4 SV=1)

HSP 1 Score: 912.9 bits (2358), Expect = 6.8e-262
Identity = 479/575 (83.30%), Postives = 511/575 (88.87%), Query Frame = 0

Query: 13  RFSIGTGQRRRSQMSNFAVQVLLGRWFTAFASLLIMSVSGASYMFALYSSDIKSSLNYDQ 72
           RFSI T  R    M NF  QVLLGRWFT FASLLIMSVSGA+YMFALYS+DIKSSL+YDQ
Sbjct: 5   RFSIRT-LRSSVPMRNFVWQVLLGRWFTVFASLLIMSVSGATYMFALYSADIKSSLDYDQ 64

Query: 73  TTLNLVGFFKDLGSNVGVFSGLINEITPPWVVLFIGGVMNFFGYFMIWLSVTHRISKPKL 132
           TTLNL+GFFKDLG+NVGVFSGLINEITPPWVVL IGGVMNFFGYFMIWLSVTHRI KPKL
Sbjct: 65  TTLNLIGFFKDLGANVGVFSGLINEITPPWVVLLIGGVMNFFGYFMIWLSVTHRIPKPKL 124

Query: 133 PAMCLFIFLGANSQTFANTGALIPSVKNFPQNRGNVLGLLKGFVGLSGAILTQIYHAFYG 192
           PAMCL+I LGANSQTFANTGALIPSVKNFPQNRGNVLGLLKGFVGLSGAILTQIYHAFYG
Sbjct: 125 PAMCLYICLGANSQTFANTGALIPSVKNFPQNRGNVLGLLKGFVGLSGAILTQIYHAFYG 184

Query: 193 DDSKDFILLIAWLPTAVSLLLLRIVRVVEVNPIFKSNDLKNFYSMLYISLGLAGFLMILI 252
           DDSKDFILLIAWLPTAVS LLLR+VRVVEVN I KS+DLKNFY MLY SL LAGFLMILI
Sbjct: 185 DDSKDFILLIAWLPTAVSFLLLRVVRVVEVNRISKSSDLKNFYYMLYTSLVLAGFLMILI 244

Query: 253 IIQNELMFTRIQYLVCVFVLLAFLFLPLVVVIREEFSIRKRKLQGVDVTSWLPVPSDQSP 312
           IIQNEL+FTRIQYL C F+LL  LFLPLVVVIREEF +RKRK Q +DV S LP+  D SP
Sbjct: 245 IIQNELIFTRIQYLGCAFILLVLLFLPLVVVIREEFLMRKRKSQDMDVISVLPL--DPSP 304

Query: 313 AELPLPRPSSLRTTDIALTNPSSCFENVFRPPERGEDYTILQAIFSVDMLILFFTTICGA 372
            EL   RP            PSSCF+NVF+PPERG+DYTILQAIFSVDMLILF  TICGA
Sbjct: 305 VELSSSRP------------PSSCFKNVFKPPERGDDYTILQAIFSVDMLILFVATICGA 364

Query: 373 GGTLTAIDNLGQIGSSLGYSTHTISTFTSLVSIWGFLGRAFSGYASEFLWTKYNFSRPLF 432
           GG LTA+DNLGQIGSSLGYS H+ISTFTSLVSIWGFLGRAFSGYASEFLWTKY+FSRPLF
Sbjct: 365 GGALTAMDNLGQIGSSLGYSAHSISTFTSLVSIWGFLGRAFSGYASEFLWTKYSFSRPLF 424

Query: 433 LTFVLLLSCFGHLLISSGVPTSIYFASVIIGFCFGAQWPLIFAIVSELFGLKYYATLYSI 492
            T VLL SC GHLLI+ GVPTS+YF+SVIIGFCFGAQWPLIFAIVSELFGLKYYATLYSI
Sbjct: 425 FTLVLLFSCVGHLLIAFGVPTSLYFSSVIIGFCFGAQWPLIFAIVSELFGLKYYATLYSI 484

Query: 493 SGIASPIGSYIFNVKVAGYLYDQEARKQIGAIGLRNVAGRDLACKGVHCYRLAFLIISAS 552
           SGIASP+GSYIFNVKVAG+LYDQEARKQ+ A GL  VAGRDLACKGVHCYRLAFLII+A+
Sbjct: 485 SGIASPVGSYIFNVKVAGFLYDQEARKQMAASGLVTVAGRDLACKGVHCYRLAFLIITAA 544

Query: 553 TMFGCFVSFILVLRTWKFYKGDIYKKFRDERKESK 588
           T+ GC VSFILVLRTW+FYKGDIYKKFR+ER E++
Sbjct: 545 TVLGCVVSFILVLRTWEFYKGDIYKKFREERTETE 564

BLAST of Cmc08g0221191 vs. TAIR 10
Match: AT2G39210.1 (Major facilitator superfamily protein )

HSP 1 Score: 641.3 bits (1653), Expect = 7.3e-184
Identity = 331/568 (58.27%), Postives = 418/568 (73.59%), Query Frame = 0

Query: 26  MSNFAVQVLLGRWFTAFASLLIMSVSGASYMFALYSSDIKSSLNYDQTTLNLVGFFKDLG 85
           M +  +Q+L GRWF  F SLLIMS +GA+YMF +YS DIK +L YDQTTLNL+ FFKDLG
Sbjct: 10  MKSLTIQILTGRWFMFFGSLLIMSTAGATYMFGIYSGDIKETLGYDQTTLNLLSFFKDLG 69

Query: 86  SNVGVFSGLINEITPPWVVLFIGGVMNFFGYFMIWLSVTHRISKPKLPAMCLFIFLGANS 145
           +NVGV +GL+NE+TPPW +L IG ++NFFGYFMIWL+VT RISKP++  MCL+I +GANS
Sbjct: 70  ANVGVLAGLLNEVTPPWFILLIGAILNFFGYFMIWLAVTERISKPQVWHMCLYICVGANS 129

Query: 146 QTFANTGALIPSVKNFPQNRGNVLGLLKGFVGLSGAILTQIYHAFYGDDSKDFILLIAWL 205
           Q+FANTG+L+  VKNFP++RG VLG+LKG+VGLSGAI+TQ+Y AFYG+D+K+ IL+I WL
Sbjct: 130 QSFANTGSLVTCVKNFPESRGVVLGILKGYVGLSGAIITQLYRAFYGEDTKELILMIGWL 189

Query: 206 PTAVSLLLLRIVRVVEVNPIFKSNDLKNFYSMLYISLGLAGFLMILIIIQNELMFTRIQY 265
           P  VS   LR +R+++V    ++N+LK FY+ LYISLGLA FLM++III     FT+ ++
Sbjct: 190 PAIVSFAFLRTIRIMKVKR--QTNELKVFYNFLYISLGLATFLMVVIIINKLSGFTQSEF 249

Query: 266 LVCVFVLLAFLFLPLVVVIREEFSIRKRKLQGVDVTSWLPVPSDQSPAELPLPRP----S 325
                V++  L LP++VVI EE  + K K   +         +D +P  +   +P    S
Sbjct: 250 GGSAAVVIVLLLLPIIVVILEEKKLWKEKQVAL---------NDPAPINVVTEKPKLDSS 309

Query: 326 SLRTTD-------IALTNPSSCFENVFRPPERGEDYTILQAIFSVDMLILFFTTICGAGG 385
             +  D       +      SC+  VF PPERG+DYTILQA+FSVDMLILF  TICG GG
Sbjct: 310 EFKDDDGEESKEVVEKVKTPSCWTTVFNPPERGDDYTILQALFSVDMLILFLATICGVGG 369

Query: 386 TLTAIDNLGQIGSSLGYSTHTISTFTSLVSIWGFLGRAFSGYASEFLWTKYNFSRPLFLT 445
           TLTAIDNLGQIG+SLGY   ++STF SLVSIW + GR  SG  SE    KY F RPL LT
Sbjct: 370 TLTAIDNLGQIGNSLGYPKRSVSTFVSLVSIWNYYGRVVSGVVSEIFLIKYKFPRPLMLT 429

Query: 446 FVLLLSCFGHLLISSGVPTSIYFASVIIGFCFGAQWPLIFAIVSELFGLKYYATLYSISG 505
            VLLLSC GHLLI+  VP  +Y ASVIIGFCFGAQWPL+FAI+SE+FGLKYY+TLY+   
Sbjct: 430 MVLLLSCAGHLLIAFNVPGGLYVASVIIGFCFGAQWPLLFAIISEIFGLKYYSTLYNFGS 489

Query: 506 IASPIGSYIFNVKVAGYLYDQEARKQIGAIGLRNVAGRDLACKGVHCYRLAFLIISASTM 565
           +ASPIGSY+ NV+VAGYLYD EA KQ  A+G   V G+DL C G  C++L+F+II+A T+
Sbjct: 490 VASPIGSYLLNVRVAGYLYDVEAGKQYKALGKTRVEGQDLNCIGTSCFKLSFIIIAAVTL 549

Query: 566 FGCFVSFILVLRTWKFYKGDIYKKFRDE 583
           FG  VS +LV+RT KFYK DIYKKFR++
Sbjct: 550 FGVLVSMVLVIRTKKFYKSDIYKKFREK 566

BLAST of Cmc08g0221191 vs. TAIR 10
Match: AT2G28120.1 (Major facilitator superfamily protein )

HSP 1 Score: 592.4 bits (1526), Expect = 3.9e-169
Identity = 308/553 (55.70%), Postives = 391/553 (70.71%), Query Frame = 0

Query: 29  FAVQVLLGRWFTAFASLLIMSVSGASYMFALYSSDIKSSLNYDQTTLNLVGFFKDLGSNV 88
           F +    GRWF  FAS LIM+ +GA+Y+F  YS DIKS+L YDQTTLNL+GFFKDLG+NV
Sbjct: 9   FLIHFFNGRWFMVFASFLIMACAGATYLFGTYSKDIKSTLGYDQTTLNLLGFFKDLGANV 68

Query: 89  GVFSGLINEITPPWVVLFIGGVMNFFGYFMIWLSVTHRISKPKLPAMCLFIFLGANSQTF 148
           GV SGLI E+TP W VL IG  MNF GYFMIWL+VT +++KPK+  MCL+I +GANSQ F
Sbjct: 69  GVLSGLIAEVTPTWFVLTIGSAMNFVGYFMIWLTVTGKVAKPKVWQMCLYICIGANSQNF 128

Query: 149 ANTGALIPSVKNFPQNRGNVLGLLKGFVGLSGAILTQIYHAFYGDDSKDFILLIAWLPTA 208
           ANTGAL+  VKNFP++RG +LGLLKG+VGLSGAI TQ+Y A YG DSK  ILLIAWLP A
Sbjct: 129 ANTGALVTCVKNFPESRGVMLGLLKGYVGLSGAIFTQLYFAIYGHDSKSLILLIAWLPAA 188

Query: 209 VSLLLLRIVRVVEVNPIFKSNDLKNFYSMLYISLGLAGFLMILIIIQNELMFTRIQYLVC 268
           VSL+ + ++R  E   + + N+L  FY  LYIS+ LA FLM + I + ++ F++  Y   
Sbjct: 189 VSLVFVYLIR--EKKVVRQRNELSVFYQFLYISIFLALFLMAMNIAEKQVHFSKAAYAAS 248

Query: 269 VFVLLAFLFLPLVVVIREEFSIRKRKLQGVDVTSWLPVPSDQSPAELPLPRPSSLRTTDI 328
             +  A LF+PL V +++E  +       ++  S + V   +   +L   + + +   + 
Sbjct: 249 ATICCALLFVPLTVSVKQELEVWNMMKLPIEEPSEVKVEKPKKELDLDQDKAAKVNGEE- 308

Query: 329 ALTNPSSCFENVFRPPERGEDYTILQAIFSVDMLILFFTTICGAGGTLTAIDNLGQIGSS 388
                 SCF  VF PP RGEDYTILQA+ S DM+ILF  T CG G +LTA+DNLGQIG S
Sbjct: 309 --KETKSCFSTVFSPPPRGEDYTILQALLSTDMIILFVATFCGLGSSLTAVDNLGQIGES 368

Query: 389 LGYSTHTISTFTSLVSIWGFLGRAFSGYASEFLWTKYNFSRPLFLTFVLLLSCFGHLLIS 448
           LGY  HT+S+F SLVSIW + GR FSG+ SE+L  KY   RPL +T VLLLSC GHLLI+
Sbjct: 369 LGYPNHTVSSFVSLVSIWNYFGRVFSGFVSEYLLAKYKLPRPLMMTLVLLLSCAGHLLIA 428

Query: 449 SGVPTSIYFASVIIGFCFGAQWPLIFAIVSELFGLKYYATLYSISGIASPIGSYIFNVKV 508
             VP S+Y AS+++GF FGAQ PL+FAI+SELFGLKYY+TL++   +ASP+GSYI NV+V
Sbjct: 429 FPVPGSVYIASILMGFSFGAQLPLLFAIISELFGLKYYSTLFNCGQLASPLGSYILNVRV 488

Query: 509 AGYLYDQEARKQIGAIGLRNVAGRDLACKGVHCYRLAFLIISASTMFGCFVSFILVLRTW 568
            G LYD+EA KQ+ A GL     +DL C G  CY+L FLI++A T FG  VS  L +RT 
Sbjct: 489 TGMLYDKEALKQLTARGLTRKDVKDLTCLGSQCYKLPFLILAAVTFFGALVSLGLAIRTR 548

Query: 569 KFYKGDIYKKFRD 582
           +FYKGDIYKKFR+
Sbjct: 549 EFYKGDIYKKFRE 556

BLAST of Cmc08g0221191 vs. TAIR 10
Match: AT1G74780.1 (Nodulin-like / Major Facilitator Superfamily protein )

HSP 1 Score: 361.3 bits (926), Expect = 1.5e-99
Identity = 219/564 (38.83%), Postives = 326/564 (57.80%), Query Frame = 0

Query: 31  VQVLLGRWFTAFASLLIMSVSGASYMFALYSSDIKSSLNYDQTTLNLVGFFKDLGSNVGV 90
           +++L  +W    AS+ I   SGASY F +YS+ +KS+ +YDQ+TL+ V  FKD+G+N GV
Sbjct: 1   MEILRTKWVAMTASIWIQCASGASYTFGIYSAVLKSTQSYDQSTLDTVSVFKDIGANAGV 60

Query: 91  FSGL----------------INEITPPWVVLFIGGVMNFFGYFMIWLSVTHRISKPKLPA 150
           FSGL                I     PWVVL +G +  F GYF+IW SVT  I KP +P 
Sbjct: 61  FSGLLYTYATSNRLRGRGGGIGGAGGPWVVLAVGAIQCFAGYFLIWASVTGLIRKPPVPL 120

Query: 151 MCLFIFLGANSQTFANTGALIPSVKNFPQNRGNVLGLLKGFVGLSGAILTQIYHAFYGDD 210
           MCLF+FL A SQTF NT  ++ +V+NF    G  +G++KGF+GLSGAIL Q+Y      D
Sbjct: 121 MCLFMFLAAQSQTFFNTANVVSAVENFADYGGTAVGIMKGFLGLSGAILIQLYETLCAGD 180

Query: 211 SKDFILLIAWLPTAVSLLLLRIVRVVEVNPIFKSNDLKNFYSMLYISLGLAGFLMILIII 270
              FILL+A  PT +SLL++ +VR+ E +    ++D K+   +  +SL +A +LMI+II+
Sbjct: 181 PASFILLLAVTPTVLSLLVMPLVRIYETS---VADDKKHLNGLSAVSLIIAAYLMIIIIL 240

Query: 271 QNELMFTRIQYLVCVFVLLAFLFLPLVVVIREEFSIRKRKLQGVDVTSWLPVPSDQSPAE 330
           +N    +    +V +  LL  L LPL++        R+ +  G++ T    VP D SP  
Sbjct: 241 KNTFGLSSWANIVTLVCLLVMLALPLLIA-------RRAQRDGMEKT----VPHDYSPL- 300

Query: 331 LPLPRPSSLRTTDIALTNPSSCFENVFRPPERGEDYTILQAIFSVDMLILFFTTICGAGG 390
             +  P +  + + +    S     +       E+  +LQA+  +   +LF   ICG G 
Sbjct: 301 --ISSPKATTSGNQSSEGDSKVEAGL------SENLNLLQAMKKLSFWLLFLAMICGMGS 360

Query: 391 TLTAIDNLGQIGSSLGYSTHTISTFTSLVSIWGFLGRAFSGYASEFLWTKYNFSRPLFLT 450
            L+ I+N+ QIG SL YS+  I++  SL SIW FLGR  +GYAS+ L  K  + RPL + 
Sbjct: 361 GLSTINNIRQIGESLRYSSVEINSLVSLWSIWNFLGRFGAGYASDALLHKKGWPRPLLMA 420

Query: 451 FVLLLSCFGHLLISSGVPTSIYFASVIIGFCFGAQWPLIFAIVSELFGLKYYATLYSISG 510
             L     GHL+I+SG   ++Y  SVI+G C+G+QW L+  I SELFG+++  T+++   
Sbjct: 421 ATLGTMSIGHLIIASGFQGNLYVGSVIVGVCYGSQWSLMPTITSELFGIRHMGTIFNTIS 480

Query: 511 IASPIGSYIFNVKVAGYLYDQEARKQIGAIGLRNVAGRDLACKGVHCYRLAFLIISASTM 570
           +ASPIGSYIF+V++ GY+YD+ A            +G    C G HC+RL+F+I+++   
Sbjct: 481 VASPIGSYIFSVRLIGYIYDKTA------------SGEGNTCYGSHCFRLSFIIMASVAF 529

Query: 571 FGCFVSFILVLRTWKFYKGDIYKK 579
           FG  V+ +L  RT   Y+  + K+
Sbjct: 541 FGFLVAIVLFFRTKTLYRQILVKR 529

BLAST of Cmc08g0221191 vs. TAIR 10
Match: AT1G18940.1 (Nodulin-like / Major Facilitator Superfamily protein )

HSP 1 Score: 343.6 bits (880), Expect = 3.2e-94
Identity = 210/569 (36.91%), Postives = 320/569 (56.24%), Query Frame = 0

Query: 30  AVQVLLGRWFTAFASLLIMSVSGASYMFALYSSDIKSSLNYDQTTLNLVGFFKDLGSNVG 89
           A ++L  +W    AS+ I   +G SY F +YS+ +KS+ +YDQ+TL+ V  FKD+G NVG
Sbjct: 2   ATEILRTKWMAMTASIWIQCSAGGSYTFGIYSAILKSTQSYDQSTLDTVSVFKDIGGNVG 61

Query: 90  VFSGLINEITP--------------PWVVLFIGGVMNFFGYFMIWLSVTHRISKPKLPAM 149
           V SGL+                   PWVV+ IG ++NF GYF++W SVT  I +P +P M
Sbjct: 62  VLSGLVYTAATFNRRRRDGRERRGGPWVVILIGAILNFTGYFLMWASVTGLIKRPPVPVM 121

Query: 150 CLFIFLGANSQTFANTGALIPSVKNFPQNRGNVLGLLKGFVGLSGAILTQIYHAFYGDDS 209
           CLF+F+ A S TF NT  ++ S++NF    G  +G++KGFVGLSGA+L Q+Y      D 
Sbjct: 122 CLFMFIAAQSLTFLNTANVVSSLENFADYGGTAVGIMKGFVGLSGAMLIQLYEVVCPGDP 181

Query: 210 KDFILLIAWLPTAVSLLLLRIVRVVEVNPIFKSNDLKNFYSMLYISLGLAGFLMILIIIQ 269
           K FILL+A +P+ +S+L++ +VRV + + +   ++ K+   +  +SL +A +LMI II++
Sbjct: 182 KTFILLLAIVPSLLSVLVMPLVRVYKTSTV---DEKKHLDGLSTLSLIIAAYLMITIILK 241

Query: 270 NELMFTRIQYLVCVFVLLAFLFLPLVVVIR-EEFSIRKRKLQGVDVTSWLPVPSDQSPAE 329
           + L        V + VLL  L  PL+V +R    SI K            P+ S  SP  
Sbjct: 242 STLSLPSWANAVTLAVLLVLLSSPLLVAVRAHRDSIEK------------PLSSVYSP-- 301

Query: 330 LPLPRPSSLRTTDIALTNPSSCFENVFRPPERGEDYTILQAIFSVDMLILFFTTICGAGG 389
                          + N  +         +  +   +LQA+ +VD  +LF   ICG G 
Sbjct: 302 --------------LVDNLEATTSGEILMLDEDKSLNLLQAMCNVDFWLLFLAMICGMGS 361

Query: 390 TLTAIDNLGQIGSSLGYSTHTISTFTSLVSIWGFLGRAFSGYASEFLWTKYNFSRPLFLT 449
            ++ I+N+ QIG SL Y++  I++  +L +IW F+GR   GY S++L  +  + RPL + 
Sbjct: 362 GISTINNIRQIGESLRYTSVEINSLLALWNIWNFIGRFGGGYVSDWLLHRKGWPRPLLMA 421

Query: 450 FVLLLSCFGHLLISSGVPTSIYFASVIIGFCFGAQWPLIFAIVSELFGLKYYATLYSISG 509
             L     GHL+I+SG   ++Y  S+I+G C+G+QW L+  I SELFG+K+  T+Y+   
Sbjct: 422 TTLGTMTIGHLIIASGFQGNLYPGSIIVGICYGSQWSLMPTITSELFGVKHMGTIYNTIS 481

Query: 510 IASPIGSYIFNVKVAGYLYDQEARKQIGAIGLRNVAGRDLACKGVHCYRLAFLIISASTM 569
           IASP+GSYIF+V++ GY+YD            R + G    C G HC+RLA+++I++   
Sbjct: 482 IASPMGSYIFSVRLIGYIYD------------RTIIGEGNTCYGPHCFRLAYVVIASVAF 526

Query: 570 FGCFVSFILVLRTWKFYKGDIYKKFRDER 584
            G  VS +LV RT   Y+  I++K    R
Sbjct: 542 LGFLVSCVLVFRTKTIYR-QIFEKILHRR 526

BLAST of Cmc08g0221191 vs. TAIR 10
Match: AT2G34355.1 (Major facilitator superfamily protein )

HSP 1 Score: 343.2 bits (879), Expect = 4.1e-94
Identity = 211/554 (38.09%), Postives = 319/554 (57.58%), Query Frame = 0

Query: 37  RWFTAFASLLIMSVSGASYMFALYSSDIKSSLNYDQTTLNLVGFFKDLGSNVGVFSGLI- 96
           +W  A AS+ I S SGA+Y FA+YSS +KSS +YDQ+TL+ V  FKD+G   G+ SG + 
Sbjct: 7   KWVAAAASIWIQSFSGATYTFAIYSSILKSSQSYDQSTLDFVSVFKDIGGTFGIISGFLY 66

Query: 97  -------NEITPPWVVLFIGGVMNFFGYFMIWLSVTHRISKPKLPAMCLFIFLGANSQTF 156
                       PWVV+F+G V  F G+F IW SV   I+ P +P MCLF+FL  +S  F
Sbjct: 67  TAMTSKSRGFGGPWVVVFVGLVQWFVGFFFIWASVVGLIAPPPVPLMCLFVFLAGHSLPF 126

Query: 157 ANTGALIPSVKNFPQNRGNVLGLLKGFVGLSGAILTQIYHAFYGDDSK--DFILLIAWLP 216
            NT  ++ + +NF Q  G  +G+++GF+GLSGAIL Q+YHA  G +     FILL+A +P
Sbjct: 127 FNTANVVTAARNFSQYGGTAVGIMQGFLGLSGAILIQLYHAVCGGEGNPATFILLLAIVP 186

Query: 217 TAVSLLLLRIVRVVEVNPIFKSNDLKNFYSMLYISLGLAGFLMILIIIQNELMFTRIQYL 276
           T V  L +  VRV E   I   +D K+   +  IS+ +A +LM++I ++N L  +R   +
Sbjct: 187 TLVMFLAMPFVRVYETVTI---SDKKHLDGLSAISMIIAAYLMVVITVENVLGLSRSMQI 246

Query: 277 VCVFVLLAFLFLPLVVVIREEFSIRKRKLQGVDVTSWLPVPSDQSPAELPLPRPSSLRTT 336
               ++L  L  PL+V +R   ++R+++               Q+ + L  P    +  T
Sbjct: 247 FSFILVLLLLASPLLVAVR---ALREKR---------------QTLSSLDGP----VLDT 306

Query: 337 DIALTNPSSCFENVFRPPER--GEDYTILQAIFSVDMLILFFTTICGAGGTLTAIDNLGQ 396
              L  PSS   N+F   +    ED  IL+A+ +V+  +LF   +CG G     ++N+ Q
Sbjct: 307 SALLDPPSS---NIFPDGDHLVAEDSNILEAMSTVNFWLLFLAMLCGMGSGFATVNNMRQ 366

Query: 397 IGSSLGYSTHTISTFTSLVSIWGFLGRAFSGYASEFLWTKYNFSRPLFLTFVLLLSCFGH 456
           IG SL YS+  +++  SL SIW FLGR  +GY S+    K+++ RP+F+   L +   GH
Sbjct: 367 IGESLRYSSVQLNSLVSLWSIWNFLGRFGAGYVSDTFLHKHSWPRPIFMAITLGVMAIGH 426

Query: 457 LLISSGVPTSIYFASVIIGFCFGAQWPLIFAIVSELFGLKYYATLYSISGIASPIGSYIF 516
           ++++SGV  S+Y  SV+IG  +G+QW L+  I SE+FG+++  T+Y    IA PIGSYI 
Sbjct: 427 IIVASGVQGSLYAGSVLIGMAYGSQWSLMPTITSEIFGIRHMGTIYFTISIAGPIGSYIL 486

Query: 517 NVKVAGYLYDQEARKQIGAIGLRNVAGRDLACKGVHCYRLAFLIISASTMFGCFVSFILV 576
           +VKV GY YD+ A +             D +C G  C+R +F+I+++  +FG  V+ +L 
Sbjct: 487 SVKVIGYFYDKVASED------------DNSCFGSQCFRTSFMIMASVALFGSLVASVLF 520

Query: 577 LRTWKFYKGDIYKK 579
            RT KFYK  + K+
Sbjct: 547 FRTHKFYKNLVAKR 520

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_008462497.10.0e+00100.00PREDICTED: uncharacterized protein LOC103500834 [Cucumis melo][more]
TYK07328.10.0e+0099.49protein NUCLEAR FUSION DEFECTIVE 4 [Cucumis melo var. makuwa][more]
KAA0063797.10.0e+0099.49protein NUCLEAR FUSION DEFECTIVE 4 [Cucumis melo var. makuwa][more]
XP_004143363.24.1e-30993.87uncharacterized protein LOC101203981 [Cucumis sativus] >KAE8647411.1 hypothetica... [more]
XP_038882767.15.8e-29290.96protein NUCLEAR FUSION DEFECTIVE 4-like [Benincasa hispida][more]
Match NameE-valueIdentityDescription
Q96TW91.7e-0426.22Probable transporter MCH1 OS=Wickerhamomyces anomalus OX=4927 GN=MCH1 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
A0A1S3CH390.0e+00100.00uncharacterized protein LOC103500834 OS=Cucumis melo OX=3656 GN=LOC103500834 PE=... [more]
A0A5D3C8490.0e+0099.49Protein NUCLEAR FUSION DEFECTIVE 4 OS=Cucumis melo var. makuwa OX=1194695 GN=E56... [more]
A0A5A7V9Y50.0e+0099.49Protein NUCLEAR FUSION DEFECTIVE 4 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C... [more]
A0A6J1CR806.3e-26884.17protein NUCLEAR FUSION DEFECTIVE 4-like OS=Momordica charantia OX=3673 GN=LOC111... [more]
A0A6J1ECZ36.8e-26283.30protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucurbita moschata OX=3662 GN=LOC1114... [more]
Match NameE-valueIdentityDescription
AT2G39210.17.3e-18458.27Major facilitator superfamily protein [more]
AT2G28120.13.9e-16955.70Major facilitator superfamily protein [more]
AT1G74780.11.5e-9938.83Nodulin-like / Major Facilitator Superfamily protein [more]
AT1G18940.13.2e-9436.91Nodulin-like / Major Facilitator Superfamily protein [more]
AT2G34355.14.1e-9438.09Major facilitator superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Melon (Charmono) v1.1
Date Performed: 2022-10-13
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR010658Nodulin-likePFAMPF06813Nodulin-likecoord: 37..284
e-value: 3.4E-87
score: 292.1
IPR036259MFS transporter superfamilyGENE3D1.20.1250.20MFS general substrate transporter like domainscoord: 351..570
e-value: 6.0E-10
score: 40.8
IPR036259MFS transporter superfamilySUPERFAMILY103473MFS general substrate transportercoord: 38..573
NoneNo IPR availablePANTHERPTHR21576:SF122MFS TRANSPORTERcoord: 21..585
NoneNo IPR availablePANTHERPTHR21576UNCHARACTERIZED NODULIN-LIKE PROTEINcoord: 21..585
NoneNo IPR availableCDDcd17354MFS_Mch1p_likecoord: 36..557
e-value: 2.21619E-103
score: 315.73

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cmc08g0221191.1Cmc08g0221191.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016020 membrane
molecular_function GO:0022857 transmembrane transporter activity