Cmc07g0205621 (gene) Melon (Charmono) v1.1

Overview


Name	Cmc07g0205621
Type	gene
Organism	Cucumis melo L. var. cantalupensis cv. Charmono (Melon (Charmono) v1.1)
Description	Trypsin inhibitor 1-like
Location	CMiso1.1chr07: 26386433 .. 26387356 (+)
RNA-Seq Expression	Cmc07g0205621
Synteny	Cmc07g0205621

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGACAGCAAGAAGATTGTTGTGGTGGCAATAGTGGGAATGATAATAATGCTAGCGGCGATGTCGTCATCGGCTTCTTTTGTCGTCGATGACAAGGACTCGACAAATCTCGTCTCGGACGGTCGAGATATGAATAATGGATGCCCAAGAAAGATAATGATAAAAGGAGGAGTGTTTAAGGAAGAACAAAGAATGTGTCCTAAAATCCTTATGAAATGCAAACAGGACTCTGACTGCTTACTTGATTGTGTTTGCCTAAAGGAAGGATTTTGTGGTTAGTGTTTAATTATAGTGTACCTCTCAAAACTATGGTGTTTTTGTATATATTTAAGTCTTTGGTTATAATGAGGATCACTCCCTCTCTGTGTATGTGATCACGTTTCTCAGTTTCAACAATATGCATAAATAAAAATCGTCATTTTCCCTTTCTTTCCTTTTAAAAGATTAGACTAATTAATTAGTTTTTTTAATGCATATTATTCTTGATAAGCAAAATGAAAGAAGACACAAATAACATAACCCGTCTCCCATCAAATACAAACTTTGTTATATAATTAAATCATCATTAACCTTCTCGTGAGAGAGAGAACAAACATAGTAATTTTAGAGAACCCAATCCCCACAATGGCCAGCGGTGTAACAAGCACAATTGGATGGGCAGTCTTTGTGAGATTTGCAAGCCTTACCTTTTACCAAACATACTCCATTTTGATCACCATTTTTCATGATATTTTTTCTTGGGAAAGCATGGCTACCGCCGTTCACAACGGCTCCAGACGCCACATTCTCAGTCGTTATGGTGGAGTCAGCTAGAGTAGCAATCATAAAGACCACCATCATCACCACCACGGTCGCCACAGAGAGGCCGGTACTATTCTTCTTGCTCTCCATTTTCTTATGCGCTTTCCTCTTCCAAGCTTTTTT

mRNA sequence

ATGGACAGCAAGAAGATTGTTGTGGTGGCAATAGTGGGAATGATAATAATGCTAGCGGCGATGTCGTCATCGGCTTCTTTTGTCGTCGATGACAAGGACTCGACAAATCTCGTCTCGGACGGTCGAGATATGAATAATGGATGCCCAAGAAAGATAATGATAAAAGGAGGAGTGTTTAAGGAAGAACAAAGAATGTGTCCTAAAATCCTTATGAAATGCAAACAGGACTCTGACTGCTTACTTGATTGTGTTTGCCTAAAGGAAGGATTTTGTGGTTAGTGTTTAATTATAGTGTACCTCTCAAAACTATGGTGTTTTTGTATATATTTAAGTCTTTGGTTATAATGAGGATCACTCCCTCTCTGTGTATGTGATCACGTTTCTCAGTTTCAACAATATGCATAAATAAAAATCGTCATTTTCCCTTTCTTTCCTTTTAAAAGATTAGACTAATTAATTAGTTTTTTTAATGCATATTATTCTTGATAAGCAAAATGAAAGAAGACACAAATAACATAACCCGTCTCCCATCAAATACAAACTTTGTTATATAATTAAATCATCATTAACCTTCTCGTGAGAGAGAGAACAAACATAGTAATTTTAGAGAACCCAATCCCCACAATGGCCAGCGGTGTAACAAGCACAATTGGATGGGCAGTCTTTGTGAGATTTGCAAGCCTTACCTTTTACCAAACATACTCCATTTTGATCACCATTTTTCATGATATTTTTTCTTGGGAAAGCATGGCTACCGCCGTTCACAACGGCTCCAGACGCCACATTCTCAGTCGTTATGGTGGAGTCAGCTAGAGTAGCAATCATAAAGACCACCATCATCACCACCACGGTCGCCACAGAGAGGCCGGTACTATTCTTCTTGCTCTCCATTTTCTTATGCGCTTTCCTCTTCCAAGCTTTTTT

Coding sequence (CDS)

ATGGACAGCAAGAAGATTGTTGTGGTGGCAATAGTGGGAATGATAATAATGCTAGCGGCGATGTCGTCATCGGCTTCTTTTGTCGTCGATGACAAGGACTCGACAAATCTCGTCTCGGACGGTCGAGATATGAATAATGGATGCCCAAGAAAGATAATGATAAAAGGAGGAGTGTTTAAGGAAGAACAAAGAATGTGTCCTAAAATCCTTATGAAATGCAAACAGGACTCTGACTGCTTACTTGATTGTGTTTGCCTAAAGGAAGGATTTTGTGGTTAG

Protein sequence

MDSKKIVVVAIVGMIIMLAAMSSSASFVVDDKDSTNLVSDGRDMNNGCPRKIMIKGGVFKEEQRMCPKILMKCKQDSDCLLDCVCLKEGFCG

Homology

[Close]

BLAST of Cmc07g0205621 vs. NCBI nr
Match: KAA0044339.1 (trypsin inhibitor 1-like [Cucumis melo var. makuwa] >TYK29468.1 trypsin inhibitor 1-like [Cucumis melo var. makuwa])

HSP 1 Score: 183.3 bits (464), Expect = 9.2e-43
Identity = 92/92 (100.00%), Postives = 92/92 (100.00%), Query Frame = 0


Query: 1  MDSKKIVVVAIVGMIIMLAAMSSSASFVVDDKDSTNLVSDGRDMNNGCPRKIMIKGGVFK 60
          MDSKKIVVVAIVGMIIMLAAMSSSASFVVDDKDSTNLVSDGRDMNNGCPRKIMIKGGVFK
Sbjct: 1  MDSKKIVVVAIVGMIIMLAAMSSSASFVVDDKDSTNLVSDGRDMNNGCPRKIMIKGGVFK 60

Query: 61 EEQRMCPKILMKCKQDSDCLLDCVCLKEGFCG 93
          EEQRMCPKILMKCKQDSDCLLDCVCLKEGFCG
Sbjct: 61 EEQRMCPKILMKCKQDSDCLLDCVCLKEGFCG 92

[Close]

BLAST of Cmc07g0205621 vs. NCBI nr
Match: KAG6576919.1 (hypothetical protein SDJN03_24493, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 103.6 bits (257), Expect = 9.3e-19
Identity = 54/92 (58.70%), Postives = 71/92 (77.17%), Query Frame = 0


Query: 1   MDSKKIVVVAIVGMIIMLAAMSSSASFVVDDKDSTNLVSDGRDMNNGCPRKIMIKGGVFK 60
           M S KIV VA++   I+L A  SSA++ +DD DS +LV DGRD+N+G PRKIM K GV  
Sbjct: 54  MGSMKIVAVALIA--ILLVATLSSAAYAIDD-DSMDLVFDGRDINHGVPRKIM-KWGVLN 113

Query: 61  EEQRMCPKILMKCKQDSDCLLDCVCLKEGFCG 93
            E+R+CPKILM+CK+DSDCL +C+CL+ G+CG
Sbjct: 114 HEERVCPKILMECKKDSDCLAECICLEHGYCG 141

[Close]

BLAST of Cmc07g0205621 vs. NCBI nr
Match: KAG7014944.1 (hypothetical protein SDJN02_22575, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 103.6 bits (257), Expect = 9.3e-19
Identity = 54/92 (58.70%), Postives = 71/92 (77.17%), Query Frame = 0


Query: 1   MDSKKIVVVAIVGMIIMLAAMSSSASFVVDDKDSTNLVSDGRDMNNGCPRKIMIKGGVFK 60
           M S KIV VA++   I+L A  SSA++ +DD DS +LV DGRD+N+G PRKIM K GV  
Sbjct: 54  MGSMKIVAVALIA--ILLVATLSSAAYAIDD-DSMDLVFDGRDINHGVPRKIM-KWGVLN 113

Query: 61  EEQRMCPKILMKCKQDSDCLLDCVCLKEGFCG 93
            E+R+CPKILM+CK+DSDCL +C+CL+ G+CG
Sbjct: 114 HEERVCPKILMECKKDSDCLAECICLEHGYCG 141

[Close]

BLAST of Cmc07g0205621 vs. NCBI nr
Match: XP_022922566.1 (probable polygalacturonase At3g15720 [Cucurbita moschata])

HSP 1 Score: 85.5 bits (210), Expect = 2.6e-13
Identity = 48/84 (57.14%), Postives = 63/84 (75.00%), Query Frame = 0


Query: 1  MDSKKIVVVAIVGMIIMLAAMSSSASFVVDDKDSTNLVSDGRDMNNGCPRKIMIKGGVFK 60
          M S KIV VA++   I+L A  SSA++ +DD DS +LV DGRD+N+  PRKIM K GV  
Sbjct: 1  MGSMKIVAVALIA--ILLVATLSSAAYAIDD-DSMDLVFDGRDINHRVPRKIM-KWGVLN 60

Query: 61 EEQRMCPKILMKCKQDSDCLLDCV 85
           E+R+CPKILM+CK+DSDCL +C+
Sbjct: 61 HEERVCPKILMECKKDSDCLAECL 80

[Close]

BLAST of Cmc07g0205621 vs. NCBI nr
Match: XP_023552846.1 (probable polygalacturonase At3g15720 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 80.9 bits (198), Expect = 6.4e-12
Identity = 45/82 (54.88%), Postives = 62/82 (75.61%), Query Frame = 0


Query: 1  MDSKKIVVVAIVGMIIMLAAMSSSASFVVDDKDSTNLVSDGRDMNNGCPRKIMIKGGVFK 60
          M S K V VA++ M+++    +SSA++ +DD DS +LV DGRD+N+G PRKIM K GV  
Sbjct: 1  MGSMKNVAVALIAMMLV---ATSSAAYAIDD-DSMDLVFDGRDINHGVPRKIM-KWGVLN 60

Query: 61 EEQRMCPKILMKCKQDSDCLLD 83
           E+R+CPKILM+CK+DSDCL +
Sbjct: 61 HEERVCPKILMECKKDSDCLAE 77

[Close]

BLAST of Cmc07g0205621 vs. ExPASy Swiss-Prot
Match: P32041 (Trypsin inhibitor 3 OS=Cucumis melo var. conomon OX=3657 PE=1 SV=1)

HSP 1 Score: 70.9 bits (172), Expect = 8.7e-12
Identity = 30/30 (100.00%), Postives = 30/30 (100.00%), Query Frame = 0


Query: 63 QRMCPKILMKCKQDSDCLLDCVCLKEGFCG 93
          QRMCPKILMKCKQDSDCLLDCVCLKEGFCG
Sbjct: 1  QRMCPKILMKCKQDSDCLLDCVCLKEGFCG 30

[Close]

BLAST of Cmc07g0205621 vs. ExPASy Swiss-Prot
Match: P07853 (Trypsin inhibitor 4 OS=Cucurbita maxima OX=3661 PE=1 SV=2)

HSP 1 Score: 59.3 bits (142), Expect = 2.6e-08
Identity = 22/31 (70.97%), Postives = 29/31 (93.55%), Query Frame = 0


Query: 62 EQRMCPKILMKCKQDSDCLLDCVCLKEGFCG 93
          E+R+CP+ILMKCK+DSDCL +CVCL+ G+CG
Sbjct: 2  EERVCPRILMKCKKDSDCLAECVCLEHGYCG 32

[Close]

BLAST of Cmc07g0205621 vs. ExPASy Swiss-Prot
Match: P10293 (Trypsin inhibitor 3 OS=Cucurbita pepo OX=3663 PE=1 SV=1)

HSP 1 Score: 58.5 bits (140), Expect = 4.5e-08
Identity = 21/31 (67.74%), Postives = 29/31 (93.55%), Query Frame = 0


Query: 62 EQRMCPKILMKCKQDSDCLLDCVCLKEGFCG 93
          E+R+CPKILM+CK+DSDCL +C+CL+ G+CG
Sbjct: 2  EERVCPKILMECKKDSDCLAECICLEHGYCG 32

[Close]

BLAST of Cmc07g0205621 vs. ExPASy Swiss-Prot
Match: P34950 (Trypsin inhibitor 5 OS=Luffa aegyptiaca OX=3670 PE=1 SV=1)

HSP 1 Score: 55.8 bits (133), Expect = 2.9e-07
Identity = 33/77 (42.86%), Postives = 44/77 (57.14%), Query Frame = 0


Query: 18 LAAMSSSASFVVDDKDSTNLVSDGRDMNNGCPRKIMI--KGGVFKEEQRMCPKILMKCKQ 77
          +A+++ S+  V    +   L+SDG    N  PRKIM    GG+       CP+ILM CK 
Sbjct: 1  MASVAESSGVV----EVIELISDG---GNDLPRKIMSGRHGGI-------CPRILMPCKT 60

Query: 78 DSDCLLDCVCLKEGFCG 93
          D DC+LDC CL  G+CG
Sbjct: 61 DDDCMLDCRCLSNGYCG 63

[Close]

BLAST of Cmc07g0205621 vs. ExPASy Swiss-Prot
Match: P01074 (Trypsin inhibitor 1 OS=Cucurbita maxima OX=3661 PE=1 SV=1)

HSP 1 Score: 55.1 bits (131), Expect = 5.0e-07
Identity = 20/29 (68.97%), Postives = 27/29 (93.10%), Query Frame = 0


Query: 64 RMCPKILMKCKQDSDCLLDCVCLKEGFCG 93
          R+CP+ILM+CK+DSDCL +CVCL+ G+CG
Sbjct: 1  RVCPRILMECKKDSDCLAECVCLEHGYCG 29

[Close]

BLAST of Cmc07g0205621 vs. ExPASy TrEMBL
Match: A0A5D3E148 (Trypsin inhibitor 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold655G00640 PE=3 SV=1)

HSP 1 Score: 183.3 bits (464), Expect = 4.5e-43
Identity = 92/92 (100.00%), Postives = 92/92 (100.00%), Query Frame = 0


Query: 1  MDSKKIVVVAIVGMIIMLAAMSSSASFVVDDKDSTNLVSDGRDMNNGCPRKIMIKGGVFK 60
          MDSKKIVVVAIVGMIIMLAAMSSSASFVVDDKDSTNLVSDGRDMNNGCPRKIMIKGGVFK
Sbjct: 1  MDSKKIVVVAIVGMIIMLAAMSSSASFVVDDKDSTNLVSDGRDMNNGCPRKIMIKGGVFK 60

Query: 61 EEQRMCPKILMKCKQDSDCLLDCVCLKEGFCG 93
          EEQRMCPKILMKCKQDSDCLLDCVCLKEGFCG
Sbjct: 61 EEQRMCPKILMKCKQDSDCLLDCVCLKEGFCG 92

[Close]

BLAST of Cmc07g0205621 vs. ExPASy TrEMBL
Match: A0A0A0KV23 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G000835 PE=3 SV=1)

HSP 1 Score: 131.7 bits (330), Expect = 1.5e-27
Identity = 71/98 (72.45%), Postives = 79/98 (80.61%), Query Frame = 0


Query: 1  MDSKKIVVVAIVGMIIMLAAMSSSASFVVDDKDSTNLVSDGR-----DMNNGCPRKIMIK 60
          M+SKKIV+VAIV  II+  AMSSSASFVVDDKD   LVSDGR     DMNNGCPRK+MIK
Sbjct: 1  MESKKIVMVAIVATIIVQVAMSSSASFVVDDKDWIKLVSDGRAQADQDMNNGCPRKMMIK 60

Query: 61 GGVFKEEQRMCPKILMKCKQDSDCLLDCVCLKE-GFCG 93
          GGVFKEE+ MCP+ILMKCK DSDCL  CVCL+   +CG
Sbjct: 61 GGVFKEEEMMCPRILMKCKHDSDCLPGCVCLEHIEYCG 98

[Close]

BLAST of Cmc07g0205621 vs. ExPASy TrEMBL
Match: A0A6J1E4H0 (probable polygalacturonase At3g15720 OS=Cucurbita moschata OX=3662 GN=LOC111430533 PE=3 SV=1)

HSP 1 Score: 85.5 bits (210), Expect = 1.3e-13
Identity = 48/84 (57.14%), Postives = 63/84 (75.00%), Query Frame = 0


Query: 1  MDSKKIVVVAIVGMIIMLAAMSSSASFVVDDKDSTNLVSDGRDMNNGCPRKIMIKGGVFK 60
          M S KIV VA++   I+L A  SSA++ +DD DS +LV DGRD+N+  PRKIM K GV  
Sbjct: 1  MGSMKIVAVALIA--ILLVATLSSAAYAIDD-DSMDLVFDGRDINHRVPRKIM-KWGVLN 60

Query: 61 EEQRMCPKILMKCKQDSDCLLDCV 85
           E+R+CPKILM+CK+DSDCL +C+
Sbjct: 61 HEERVCPKILMECKKDSDCLAECL 80

[Close]

BLAST of Cmc07g0205621 vs. ExPASy TrEMBL
Match: Q9S8W2 (CMCTI-III=TRYPSIN inhibitor OS=Cucumis melo OX=3656 PE=3 SV=1)

HSP 1 Score: 69.7 bits (169), Expect = 7.2e-09
Identity = 29/30 (96.67%), Postives = 30/30 (100.00%), Query Frame = 0


Query: 63 QRMCPKILMKCKQDSDCLLDCVCLKEGFCG 93
          +RMCPKILMKCKQDSDCLLDCVCLKEGFCG
Sbjct: 1  ERMCPKILMKCKQDSDCLLDCVCLKEGFCG 30

[Close]

BLAST of Cmc07g0205621 vs. ExPASy TrEMBL
Match: Q9S8W3 (CMCTI-I=TRYPSIN inhibitor OS=Cucumis melo OX=3656 PE=3 SV=1)

HSP 1 Score: 67.0 bits (162), Expect = 4.7e-08
Identity = 28/28 (100.00%), Postives = 28/28 (100.00%), Query Frame = 0


Query: 65 MCPKILMKCKQDSDCLLDCVCLKEGFCG 93
          MCPKILMKCKQDSDCLLDCVCLKEGFCG
Sbjct: 1  MCPKILMKCKQDSDCLLDCVCLKEGFCG 28

The following BLAST results are available for this feature:

BLAST of Cmc07g0205621 vs. NCBI nr


Match Name	E-value	Identity	Description
KAA0044339.1	9.2e-43	100.00	trypsin inhibitor 1-like [Cucumis melo var. makuwa] >TYK29468.1 trypsin inhibito...	[more]
KAG6576919.1	9.3e-19	58.70	hypothetical protein SDJN03_24493, partial [Cucurbita argyrosperma subsp. sorori...	[more]
KAG7014944.1	9.3e-19	58.70	hypothetical protein SDJN02_22575, partial [Cucurbita argyrosperma subsp. argyro...	[more]
XP_022922566.1	2.6e-13	57.14	probable polygalacturonase At3g15720 [Cucurbita moschata]	[more]
XP_023552846.1	6.4e-12	54.88	probable polygalacturonase At3g15720 [Cucurbita pepo subsp. pepo]	[more]

BLAST of Cmc07g0205621 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P32041	8.7e-12	100.00	Trypsin inhibitor 3 OS=Cucumis melo var. conomon OX=3657 PE=1 SV=1	[more]
P07853	2.6e-08	70.97	Trypsin inhibitor 4 OS=Cucurbita maxima OX=3661 PE=1 SV=2	[more]
P10293	4.5e-08	67.74	Trypsin inhibitor 3 OS=Cucurbita pepo OX=3663 PE=1 SV=1	[more]
P34950	2.9e-07	42.86	Trypsin inhibitor 5 OS=Luffa aegyptiaca OX=3670 PE=1 SV=1	[more]
P01074	5.0e-07	68.97	Trypsin inhibitor 1 OS=Cucurbita maxima OX=3661 PE=1 SV=1	[more]

BLAST of Cmc07g0205621 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5D3E148	4.5e-43	100.00	Trypsin inhibitor 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffol...	[more]
A0A0A0KV23	1.5e-27	72.45	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G000835 PE=3 SV=1	[more]
A0A6J1E4H0	1.3e-13	57.14	probable polygalacturonase At3g15720 OS=Cucurbita moschata OX=3662 GN=LOC1114305...	[more]
Q9S8W2	7.2e-09	96.67	CMCTI-III=TRYPSIN inhibitor OS=Cucumis melo OX=3656 PE=3 SV=1	[more]
Q9S8W3	4.7e-08	100.00	CMCTI-I=TRYPSIN inhibitor OS=Cucumis melo OX=3656 PE=3 SV=1	[more]

BLAST of Cmc07g0205621 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description

InterPro

Analysis Name: InterPro Annotations of Melon (Charmono) v1.1
Date Performed: 2022-10-13


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000737	Proteinase inhibitor I7, squash	PRINTS	PR00293	SQUASHINHBTR	coord: 66..75 score: 69.5 coord: 76..85 score: 75.0
IPR000737	Proteinase inhibitor I7, squash	PFAM	PF00299	Squash	coord: 64..92 e-value: 3.1E-17 score: 62.3
IPR000737	Proteinase inhibitor I7, squash	PROSITE	PS00286	SQUASH_INHIBITOR	coord: 66..85
IPR000737	Proteinase inhibitor I7, squash	CDD	cd00150	PlantTI	coord: 66..92 e-value: 2.93151E-11 score: 51.2243
None	No IPR available	SMART	SM00286	PTI_1	coord: 64..92 e-value: 1.3E-12 score: 57.9
None	No IPR available	GENE3D	4.10.75.20		coord: 57..92 e-value: 6.4E-15 score: 56.7
IPR011052	Proteinase/amylase inhibitor domain superfamily	SUPERFAMILY	57027	Plant inhibitors of proteinases and amylases	coord: 61..92

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cmc07g0205621.1	Cmc07g0205621.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0010951	negative regulation of endopeptidase activity
cellular_component	GO:0005576	extracellular region
cellular_component	GO:0016020	membrane
molecular_function	GO:0016798	hydrolase activity, acting on glycosyl bonds
molecular_function	GO:0004867	serine-type endopeptidase inhibitor activity
molecular_function	GO:0004866	endopeptidase inhibitor activity