Cmc07g0194031 (gene) Melon (Charmono) v1.1

Overview
NameCmc07g0194031
Typegene
OrganismCucumis melo L. var. cantalupensis cv. Charmono (Melon (Charmono) v1.1)
DescriptionGag/pol protein
LocationCMiso1.1chr07: 16335894 .. 16336271 (+)
RNA-Seq ExpressionCmc07g0194031
SyntenyCmc07g0194031
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGACCAAAAGATCTTTTACTGGAAAAGATCTTAGAGCCAAAACATCTTTAGAGCTCGTACGTTCGAACTTATGTGGACCAATGAATGTCAAAGCTCGGGGAGGATATAAATATTTCATTAGTTTTATTGATGATTATTCAAGGTATGGTCATGTTTACCTAATTCAGAACAAGTTTGATTCTTTTGAAAAGTTCAAAGAATATAAGGCTGAAGTTGAAAATGAATTAGGTAAAACAATAAAGGCACTTCGATCAGATCGAGGTGGAGAGTATATGGACTTGCGATTCCAAGACTATTTGATAGAACATGGAATCCAATCACAACTCTCTGCACCTAGTACGCCTCAGCAGAACGGTGTATCAAAAAGAAGAAACTGA

mRNA sequence

ATGACCAAAAGATCTTTTACTGGAAAAGATCTTAGAGCCAAAACATCTTTAGAGCTCGTACGTTCGAACTTATGTGGACCAATGAATGTCAAAGCTCGGGGAGGATATAAATATTTCATTAGTTTTATTGATGATTATTCAAGGTATGGTCATGTTTACCTAATTCAGAACAAGTTTGATTCTTTTGAAAAGTTCAAAGAATATAAGGCTGAAGTTGAAAATGAATTAGGTAAAACAATAAAGGCACTTCGATCAGATCGAGGTGGAGAGTATATGGACTTGCGATTCCAAGACTATTTGATAGAACATGGAATCCAATCACAACTCTCTGCACCTAGTACGCCTCAGCAGAACGGTGTATCAAAAAGAAGAAACTGA

Coding sequence (CDS)

ATGACCAAAAGATCTTTTACTGGAAAAGATCTTAGAGCCAAAACATCTTTAGAGCTCGTACGTTCGAACTTATGTGGACCAATGAATGTCAAAGCTCGGGGAGGATATAAATATTTCATTAGTTTTATTGATGATTATTCAAGGTATGGTCATGTTTACCTAATTCAGAACAAGTTTGATTCTTTTGAAAAGTTCAAAGAATATAAGGCTGAAGTTGAAAATGAATTAGGTAAAACAATAAAGGCACTTCGATCAGATCGAGGTGGAGAGTATATGGACTTGCGATTCCAAGACTATTTGATAGAACATGGAATCCAATCACAACTCTCTGCACCTAGTACGCCTCAGCAGAACGGTGTATCAAAAAGAAGAAACTGA

Protein sequence

MTKRSFTGKDLRAKTSLELVRSNLCGPMNVKARGGYKYFISFIDDYSRYGHVYLIQNKFDSFEKFKEYKAEVENELGKTIKALRSDRGGEYMDLRFQDYLIEHGIQSQLSAPSTPQQNGVSKRRN
Homology
BLAST of Cmc07g0194031 vs. NCBI nr
Match: TYK14710.1 (gag/pol protein [Cucumis melo var. makuwa])

HSP 1 Score: 240.0 bits (611), Expect = 1.1e-59
Identity = 119/125 (95.20%), Postives = 120/125 (96.00%), Query Frame = 0

Query: 1   MTKRSFTGKDLRAKTSLELVRSNLCGPMNVKARGGYKYFISFIDDYSRYGHVYLIQNKFD 60
           M KRSF GKDLRAKTSLELVRSNLCGPMNVKARGGY+YFISFIDDYSRYGHVYLIQNK D
Sbjct: 1   MIKRSFPGKDLRAKTSLELVRSNLCGPMNVKARGGYEYFISFIDDYSRYGHVYLIQNKSD 60

Query: 61  SFEKFKEYKAEVENELGKTIKALRSDRGGEYMDLRFQDYLIEHGIQSQLSAPSTPQQNGV 120
           SFEKFKEYKAEVENELGKTIK LRSDRGGEYMDLRFQDYLIEHGIQSQL APSTPQQNGV
Sbjct: 61  SFEKFKEYKAEVENELGKTIKTLRSDRGGEYMDLRFQDYLIEHGIQSQLCAPSTPQQNGV 120

Query: 121 SKRRN 126
           SKRRN
Sbjct: 121 SKRRN 125

BLAST of Cmc07g0194031 vs. NCBI nr
Match: KAA0067819.1 (gag/pol protein [Cucumis melo var. makuwa])

HSP 1 Score: 227.3 bits (578), Expect = 7.6e-56
Identity = 112/125 (89.60%), Postives = 117/125 (93.60%), Query Frame = 0

Query: 1   MTKRSFTGKDLRAKTSLELVRSNLCGPMNVKARGGYKYFISFIDDYSRYGHVYLIQNKFD 60
           MTKRSFTGK LRAKT LELV S+LC PMNVKARGGY+YFISFIDDYSRYGHVYLIQNK D
Sbjct: 306 MTKRSFTGKGLRAKTPLELVHSDLCEPMNVKARGGYEYFISFIDDYSRYGHVYLIQNKSD 365

Query: 61  SFEKFKEYKAEVENELGKTIKALRSDRGGEYMDLRFQDYLIEHGIQSQLSAPSTPQQNGV 120
           SFEKFKEYKAEVENE GKT+K  RSD+GGEYMDLRFQDYLIEHGIQSQLSAPSTPQQNGV
Sbjct: 366 SFEKFKEYKAEVENESGKTLKTFRSDQGGEYMDLRFQDYLIEHGIQSQLSAPSTPQQNGV 425

Query: 121 SKRRN 126
           S+RRN
Sbjct: 426 SERRN 430

BLAST of Cmc07g0194031 vs. NCBI nr
Match: KAA0040307.1 (gag/pol protein [Cucumis melo var. makuwa] >TYK23398.1 gag/pol protein [Cucumis melo var. makuwa])

HSP 1 Score: 224.9 bits (572), Expect = 3.8e-55
Identity = 111/125 (88.80%), Postives = 116/125 (92.80%), Query Frame = 0

Query: 1   MTKRSFTGKDLRAKTSLELVRSNLCGPMNVKARGGYKYFISFIDDYSRYGHVYLIQNKFD 60
           MTKRSFTGK L+AKT LEL+ SNLC PMNVKARGGY+YFI+FIDDYSRYGHVYLIQNK D
Sbjct: 286 MTKRSFTGKGLQAKTPLELIHSNLCEPMNVKARGGYEYFINFIDDYSRYGHVYLIQNKSD 345

Query: 61  SFEKFKEYKAEVENELGKTIKALRSDRGGEYMDLRFQDYLIEHGIQSQLSAPSTPQQNGV 120
           SFEKFKEYKAEVENE GK IK LRSDRGGEYMDLRFQDYLIEHGIQSQLSAPSTPQ NGV
Sbjct: 346 SFEKFKEYKAEVENESGKKIKTLRSDRGGEYMDLRFQDYLIEHGIQSQLSAPSTPQLNGV 405

Query: 121 SKRRN 126
           S+RRN
Sbjct: 406 SERRN 410

BLAST of Cmc07g0194031 vs. NCBI nr
Match: KAA0054875.1 (gag/pol protein [Cucumis melo var. makuwa] >TYJ96592.1 gag/pol protein [Cucumis melo var. makuwa])

HSP 1 Score: 223.8 bits (569), Expect = 8.4e-55
Identity = 111/125 (88.80%), Postives = 116/125 (92.80%), Query Frame = 0

Query: 1   MTKRSFTGKDLRAKTSLELVRSNLCGPMNVKARGGYKYFISFIDDYSRYGHVYLIQNKFD 60
           +TKRSFTGK L AKT LELV S+LCGPMNVKARGGY+YFISFIDDY+RYGHVYLIQNK D
Sbjct: 229 VTKRSFTGKGLGAKTLLELVHSDLCGPMNVKARGGYEYFISFIDDYTRYGHVYLIQNKSD 288

Query: 61  SFEKFKEYKAEVENELGKTIKALRSDRGGEYMDLRFQDYLIEHGIQSQLSAPSTPQQNGV 120
           SFEKFKEYKAEVENE GKTIK  RSDRGGEYMDLRFQDYLIEH IQSQLSAPSTPQQNGV
Sbjct: 289 SFEKFKEYKAEVENESGKTIKTFRSDRGGEYMDLRFQDYLIEHEIQSQLSAPSTPQQNGV 348

Query: 121 SKRRN 126
           S+RRN
Sbjct: 349 SERRN 353

BLAST of Cmc07g0194031 vs. NCBI nr
Match: TYK14486.1 (gag/pol protein [Cucumis melo var. makuwa])

HSP 1 Score: 223.0 bits (567), Expect = 1.4e-54
Identity = 108/125 (86.40%), Postives = 118/125 (94.40%), Query Frame = 0

Query: 1   MTKRSFTGKDLRAKTSLELVRSNLCGPMNVKARGGYKYFISFIDDYSRYGHVYLIQNKFD 60
           MTKRSFTGK LRAKT L+L+ S+LCGPMNVKARGGY+YFISFIDDYSRYGH+YLIQNK D
Sbjct: 1   MTKRSFTGKCLRAKTHLKLIHSDLCGPMNVKARGGYEYFISFIDDYSRYGHIYLIQNKSD 60

Query: 61  SFEKFKEYKAEVENELGKTIKALRSDRGGEYMDLRFQDYLIEHGIQSQLSAPSTPQQNGV 120
           SFEKFKEYKA+VENE GKTIK LRSD+GG+YMDL+FQDYLIEHGIQSQLSAPS PQQNGV
Sbjct: 61  SFEKFKEYKADVENESGKTIKTLRSDQGGKYMDLQFQDYLIEHGIQSQLSAPSMPQQNGV 120

Query: 121 SKRRN 126
           S+RRN
Sbjct: 121 SERRN 125

BLAST of Cmc07g0194031 vs. ExPASy Swiss-Prot
Match: P10978 (Retrovirus-related Pol polyprotein from transposon TNT 1-94 OS=Nicotiana tabacum OX=4097 PE=2 SV=1)

HSP 1 Score: 114.4 bits (285), Expect = 9.4e-25
Identity = 55/121 (45.45%), Postives = 79/121 (65.29%), Query Frame = 0

Query: 5   SFTGKDLRAKTSLELVRSNLCGPMNVKARGGYKYFISFIDDYSRYGHVYLIQNKFDSFEK 64
           SF     R    L+LV S++CGPM +++ GG KYF++FIDD SR   VY+++ K   F+ 
Sbjct: 469 SFQTSSERKLNILDLVYSDVCGPMEIESMGGNKYFVTFIDDASRKLWVYILKTKDQVFQV 528

Query: 65  FKEYKAEVENELGKTIKALRSDRGGEYMDLRFQDYLIEHGIQSQLSAPSTPQQNGVSKRR 124
           F+++ A VE E G+ +K LRSD GGEY    F++Y   HGI+ + + P TPQ NGV++R 
Sbjct: 529 FQKFHALVERETGRKLKRLRSDNGGEYTSREFEEYCSSHGIRHEKTVPGTPQHNGVAERM 588

Query: 125 N 126
           N
Sbjct: 589 N 589

BLAST of Cmc07g0194031 vs. ExPASy Swiss-Prot
Match: Q94HW2 (Retrovirus-related Pol polyprotein from transposon RE1 OS=Arabidopsis thaliana OX=3702 GN=RE1 PE=2 SV=1)

HSP 1 Score: 64.7 bits (156), Expect = 8.5e-10
Identity = 37/123 (30.08%), Postives = 65/123 (52.85%), Query Frame = 0

Query: 3   KRSFTGKDLRAKTSLELVRSNLCGPMNVKARGGYKYFISFIDDYSRYGHVYLIQNKFDSF 62
           K  F+   + +   LE + S++     + +   Y+Y++ F+D ++RY  +Y ++ K    
Sbjct: 510 KVPFSQSTINSTRPLEYIYSDVWS-SPILSHDNYRYYVIFVDHFTRYTWLYPLKQKSQVK 569

Query: 63  EKFKEYKAEVENELGKTIKALRSDRGGEYMDLRFQDYLIEHGIQSQLSAPSTPQQNGVSK 122
           E F  +K  +EN     I    SD GGE++ L   +Y  +HGI    S P TP+ NG+S+
Sbjct: 570 ETFITFKNLLENRFQTRIGTFYSDNGGEFVAL--WEYFSQHGISHLTSPPHTPEHNGLSE 629

Query: 123 RRN 126
           R++
Sbjct: 630 RKH 629

BLAST of Cmc07g0194031 vs. ExPASy TrEMBL
Match: A0A5D3CTE2 (Gag/pol protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold692G00400 PE=4 SV=1)

HSP 1 Score: 240.0 bits (611), Expect = 5.5e-60
Identity = 119/125 (95.20%), Postives = 120/125 (96.00%), Query Frame = 0

Query: 1   MTKRSFTGKDLRAKTSLELVRSNLCGPMNVKARGGYKYFISFIDDYSRYGHVYLIQNKFD 60
           M KRSF GKDLRAKTSLELVRSNLCGPMNVKARGGY+YFISFIDDYSRYGHVYLIQNK D
Sbjct: 1   MIKRSFPGKDLRAKTSLELVRSNLCGPMNVKARGGYEYFISFIDDYSRYGHVYLIQNKSD 60

Query: 61  SFEKFKEYKAEVENELGKTIKALRSDRGGEYMDLRFQDYLIEHGIQSQLSAPSTPQQNGV 120
           SFEKFKEYKAEVENELGKTIK LRSDRGGEYMDLRFQDYLIEHGIQSQL APSTPQQNGV
Sbjct: 61  SFEKFKEYKAEVENELGKTIKTLRSDRGGEYMDLRFQDYLIEHGIQSQLCAPSTPQQNGV 120

Query: 121 SKRRN 126
           SKRRN
Sbjct: 121 SKRRN 125

BLAST of Cmc07g0194031 vs. ExPASy TrEMBL
Match: A0A5A7VRN6 (Gag/pol protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold2484G00230 PE=4 SV=1)

HSP 1 Score: 227.3 bits (578), Expect = 3.7e-56
Identity = 112/125 (89.60%), Postives = 117/125 (93.60%), Query Frame = 0

Query: 1   MTKRSFTGKDLRAKTSLELVRSNLCGPMNVKARGGYKYFISFIDDYSRYGHVYLIQNKFD 60
           MTKRSFTGK LRAKT LELV S+LC PMNVKARGGY+YFISFIDDYSRYGHVYLIQNK D
Sbjct: 306 MTKRSFTGKGLRAKTPLELVHSDLCEPMNVKARGGYEYFISFIDDYSRYGHVYLIQNKSD 365

Query: 61  SFEKFKEYKAEVENELGKTIKALRSDRGGEYMDLRFQDYLIEHGIQSQLSAPSTPQQNGV 120
           SFEKFKEYKAEVENE GKT+K  RSD+GGEYMDLRFQDYLIEHGIQSQLSAPSTPQQNGV
Sbjct: 366 SFEKFKEYKAEVENESGKTLKTFRSDQGGEYMDLRFQDYLIEHGIQSQLSAPSTPQQNGV 425

Query: 121 SKRRN 126
           S+RRN
Sbjct: 426 SERRN 430

BLAST of Cmc07g0194031 vs. ExPASy TrEMBL
Match: A0A5A7TFI0 (Gag/pol protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold142G004640 PE=4 SV=1)

HSP 1 Score: 224.9 bits (572), Expect = 1.8e-55
Identity = 111/125 (88.80%), Postives = 116/125 (92.80%), Query Frame = 0

Query: 1   MTKRSFTGKDLRAKTSLELVRSNLCGPMNVKARGGYKYFISFIDDYSRYGHVYLIQNKFD 60
           MTKRSFTGK L+AKT LEL+ SNLC PMNVKARGGY+YFI+FIDDYSRYGHVYLIQNK D
Sbjct: 286 MTKRSFTGKGLQAKTPLELIHSNLCEPMNVKARGGYEYFINFIDDYSRYGHVYLIQNKSD 345

Query: 61  SFEKFKEYKAEVENELGKTIKALRSDRGGEYMDLRFQDYLIEHGIQSQLSAPSTPQQNGV 120
           SFEKFKEYKAEVENE GK IK LRSDRGGEYMDLRFQDYLIEHGIQSQLSAPSTPQ NGV
Sbjct: 346 SFEKFKEYKAEVENESGKKIKTLRSDRGGEYMDLRFQDYLIEHGIQSQLSAPSTPQLNGV 405

Query: 121 SKRRN 126
           S+RRN
Sbjct: 406 SERRN 410

BLAST of Cmc07g0194031 vs. ExPASy TrEMBL
Match: A0A5A7UMM9 (Gag/pol protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1278G00100 PE=4 SV=1)

HSP 1 Score: 223.8 bits (569), Expect = 4.0e-55
Identity = 111/125 (88.80%), Postives = 116/125 (92.80%), Query Frame = 0

Query: 1   MTKRSFTGKDLRAKTSLELVRSNLCGPMNVKARGGYKYFISFIDDYSRYGHVYLIQNKFD 60
           +TKRSFTGK L AKT LELV S+LCGPMNVKARGGY+YFISFIDDY+RYGHVYLIQNK D
Sbjct: 229 VTKRSFTGKGLGAKTLLELVHSDLCGPMNVKARGGYEYFISFIDDYTRYGHVYLIQNKSD 288

Query: 61  SFEKFKEYKAEVENELGKTIKALRSDRGGEYMDLRFQDYLIEHGIQSQLSAPSTPQQNGV 120
           SFEKFKEYKAEVENE GKTIK  RSDRGGEYMDLRFQDYLIEH IQSQLSAPSTPQQNGV
Sbjct: 289 SFEKFKEYKAEVENESGKTIKTFRSDRGGEYMDLRFQDYLIEHEIQSQLSAPSTPQQNGV 348

Query: 121 SKRRN 126
           S+RRN
Sbjct: 349 SERRN 353

BLAST of Cmc07g0194031 vs. ExPASy TrEMBL
Match: A0A5A7UC33 (Gag/pol protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold1184G00050 PE=4 SV=1)

HSP 1 Score: 223.0 bits (567), Expect = 6.9e-55
Identity = 108/125 (86.40%), Postives = 118/125 (94.40%), Query Frame = 0

Query: 1   MTKRSFTGKDLRAKTSLELVRSNLCGPMNVKARGGYKYFISFIDDYSRYGHVYLIQNKFD 60
           MTKRSFTGK LRAKT L+L+ S+LCGPMNVKARGGY+YFISFIDDYSRYGH+YLIQNK D
Sbjct: 1   MTKRSFTGKCLRAKTHLKLIHSDLCGPMNVKARGGYEYFISFIDDYSRYGHIYLIQNKSD 60

Query: 61  SFEKFKEYKAEVENELGKTIKALRSDRGGEYMDLRFQDYLIEHGIQSQLSAPSTPQQNGV 120
           SFEKFKEYKA+VENE GKTIK LRSD+GG+YMDL+FQDYLIEHGIQSQLSAPS PQQNGV
Sbjct: 61  SFEKFKEYKADVENESGKTIKTLRSDQGGKYMDLQFQDYLIEHGIQSQLSAPSMPQQNGV 120

Query: 121 SKRRN 126
           S+RRN
Sbjct: 121 SERRN 125

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
TYK14710.11.1e-5995.20gag/pol protein [Cucumis melo var. makuwa][more]
KAA0067819.17.6e-5689.60gag/pol protein [Cucumis melo var. makuwa][more]
KAA0040307.13.8e-5588.80gag/pol protein [Cucumis melo var. makuwa] >TYK23398.1 gag/pol protein [Cucumis ... [more]
KAA0054875.18.4e-5588.80gag/pol protein [Cucumis melo var. makuwa] >TYJ96592.1 gag/pol protein [Cucumis ... [more]
TYK14486.11.4e-5486.40gag/pol protein [Cucumis melo var. makuwa][more]
Match NameE-valueIdentityDescription
P109789.4e-2545.45Retrovirus-related Pol polyprotein from transposon TNT 1-94 OS=Nicotiana tabacum... [more]
Q94HW28.5e-1030.08Retrovirus-related Pol polyprotein from transposon RE1 OS=Arabidopsis thaliana O... [more]
Match NameE-valueIdentityDescription
A0A5D3CTE25.5e-6095.20Gag/pol protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold692G0040... [more]
A0A5A7VRN63.7e-5689.60Gag/pol protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold2484G002... [more]
A0A5A7TFI01.8e-5588.80Gag/pol protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold142G0046... [more]
A0A5A7UMM94.0e-5588.80Gag/pol protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1278G001... [more]
A0A5A7UC336.9e-5586.40Gag/pol protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold1184G000... [more]
Match NameE-valueIdentityDescription
InterPro
Analysis Name: InterPro Annotations of Melon (Charmono) v1.1
Date Performed: 2022-10-13
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001584Integrase, catalytic corePFAMPF00665rvecoord: 27..115
e-value: 8.7E-10
score: 38.8
IPR001584Integrase, catalytic corePROSITEPS50994INTEGRASEcoord: 12..125
score: 16.239166
IPR036397Ribonuclease H superfamilyGENE3D3.30.420.10coord: 8..125
e-value: 2.1E-21
score: 78.2
NoneNo IPR availablePANTHERPTHR11439:SF324RIBONUCLEASE H-LIKE DOMAIN, GAG-PRE-INTEGRASE DOMAIN, GAG-POLYPEPTIDE OF LTR COPIA-TYPE-RELATEDcoord: 22..115
NoneNo IPR availablePANTHERPTHR11439GAG-POL-RELATED RETROTRANSPOSONcoord: 22..115
IPR012337Ribonuclease H-like superfamilySUPERFAMILY53098Ribonuclease H-likecoord: 10..125

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cmc07g0194031.1Cmc07g0194031.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0015074 DNA integration
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0008270 zinc ion binding