Cmc07g0192221 (gene) Melon (Charmono) v1.1

Overview
NameCmc07g0192221
Typegene
OrganismCucumis melo L. var. cantalupensis cv. Charmono (Melon (Charmono) v1.1)
DescriptionReverse transcriptase
LocationCMiso1.1chr07: 12728181 .. 12728936 (+)
RNA-Seq ExpressionCmc07g0192221
SyntenyCmc07g0192221
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTCTTTTGGGTTGACTAATGCTCCTGCGGTATTCATGGACTTGATGAACAGGGTGTTTAAGGACTTCCTAGACTCATTCGTCATAGTTTTCATTGACGACATTTTGATTTACTCCAAAACTGAGGCTGAGCATGAGAAGCACTTGCACCAGGTTTTGAAGACTCTTCGAGCCAACAAGCTGTATGCCAAGTTCTCCAAGTGTGAGTTCTTATTAAAGAAGGTGACGTTCCTCAGCCACGTGGTTTCCAGTGAGGGAGTTTCTGTAGACCCAGCAAAGATCGAAGCAGTTACTAGTTGGCCTCGACCGTCGATAGTTAGCGAGATTCGTAGTTTCCTGGGTTTGGCAGGTTACTACCGGAGGTTTGTGGAAGACTTCTCTCGTATAGCCAGTCCCTTAACTCAGTTGACCAGGAAGGGGACCCCTTTTGTTTGGAGCCCAGCTTGCGAGAGTAGCTTCCAGGAGCTTAAGCGGAAGCTGGTGTCTGCGCCAGTCCTGACAGTGCCCGATGGGTCGGGAAGCTTTATGATCTACAGTGATGCCTCCAAAAAGAGACTGGGCTGTGTTCTGATGCAGCAAGGTAAGGTGGTTGCTTATGCCTCCTGTCAGTTGAAGAGTCATGAGCAGAACTACCCTACCCACGATTTAGAGTTGGCAGCAGTGGTCTTTGCACTGAAGATATGGAGGGACTACCTGTACGGTGAGAAGATACAGATTTTCACTGACCATAAGAGCATGAAGTACTTCTTCACCTAG

mRNA sequence

ATGTCTTTTGGGTTGACTAATGCTCCTGCGGTATTCATGGACTTGATGAACAGGGTGTTTAAGGACTTCCTAGACTCATTCGTCATAGTTTTCATTGACGACATTTTGATTTACTCCAAAACTGAGGCTGAGCATGAGAAGCACTTGCACCAGGTTTTGAAGACTCTTCGAGCCAACAAGCTGTATGCCAAGTTCTCCAAGTGTGAGTTCTTATTAAAGAAGGTGACGTTCCTCAGCCACGTGGTTTCCAGTGAGGGAGTTTCTGTAGACCCAGCAAAGATCGAAGCAGTTACTAGTTGGCCTCGACCGTCGATAGTTAGCGAGATTCGTAGTTTCCTGGGTTTGGCAGGTTACTACCGGAGGTTTGTGGAAGACTTCTCTCGTATAGCCAGTCCCTTAACTCAGTTGACCAGGAAGGGGACCCCTTTTGTTTGGAGCCCAGCTTGCGAGAGTAGCTTCCAGGAGCTTAAGCGGAAGCTGGTGTCTGCGCCAGTCCTGACAGTGCCCGATGGGTCGGGAAGCTTTATGATCTACAGTGATGCCTCCAAAAAGAGACTGGGCTGTGTTCTGATGCAGCAAGGTAAGGTGGTTGCTTATGCCTCCTGTCAGTTGAAGAGTCATGAGCAGAACTACCCTACCCACGATTTAGAGTTGGCAGCAGTGGTCTTTGCACTGAAGATATGGAGGGACTACCTGTACGGTGAGAAGATACAGATTTTCACTGACCATAAGAGCATGAAGTACTTCTTCACCTAG

Coding sequence (CDS)

ATGTCTTTTGGGTTGACTAATGCTCCTGCGGTATTCATGGACTTGATGAACAGGGTGTTTAAGGACTTCCTAGACTCATTCGTCATAGTTTTCATTGACGACATTTTGATTTACTCCAAAACTGAGGCTGAGCATGAGAAGCACTTGCACCAGGTTTTGAAGACTCTTCGAGCCAACAAGCTGTATGCCAAGTTCTCCAAGTGTGAGTTCTTATTAAAGAAGGTGACGTTCCTCAGCCACGTGGTTTCCAGTGAGGGAGTTTCTGTAGACCCAGCAAAGATCGAAGCAGTTACTAGTTGGCCTCGACCGTCGATAGTTAGCGAGATTCGTAGTTTCCTGGGTTTGGCAGGTTACTACCGGAGGTTTGTGGAAGACTTCTCTCGTATAGCCAGTCCCTTAACTCAGTTGACCAGGAAGGGGACCCCTTTTGTTTGGAGCCCAGCTTGCGAGAGTAGCTTCCAGGAGCTTAAGCGGAAGCTGGTGTCTGCGCCAGTCCTGACAGTGCCCGATGGGTCGGGAAGCTTTATGATCTACAGTGATGCCTCCAAAAAGAGACTGGGCTGTGTTCTGATGCAGCAAGGTAAGGTGGTTGCTTATGCCTCCTGTCAGTTGAAGAGTCATGAGCAGAACTACCCTACCCACGATTTAGAGTTGGCAGCAGTGGTCTTTGCACTGAAGATATGGAGGGACTACCTGTACGGTGAGAAGATACAGATTTTCACTGACCATAAGAGCATGAAGTACTTCTTCACCTAG

Protein sequence

MSFGLTNAPAVFMDLMNRVFKDFLDSFVIVFIDDILIYSKTEAEHEKHLHQVLKTLRANKLYAKFSKCEFLLKKVTFLSHVVSSEGVSVDPAKIEAVTSWPRPSIVSEIRSFLGLAGYYRRFVEDFSRIASPLTQLTRKGTPFVWSPACESSFQELKRKLVSAPVLTVPDGSGSFMIYSDASKKRLGCVLMQQGKVVAYASCQLKSHEQNYPTHDLELAAVVFALKIWRDYLYGEKIQIFTDHKSMKYFFT
Homology
BLAST of Cmc07g0192221 vs. NCBI nr
Match: TYK05193.1 (pol protein [Cucumis melo var. makuwa])

HSP 1 Score: 473.0 bits (1216), Expect = 1.6e-129
Identity = 235/251 (93.63%), Postives = 244/251 (97.21%), Query Frame = 0

Query: 1    MSFGLTNAPAVFMDLMNRVFKDFLDSFVIVFIDDILIYSKTEAEHEKHLHQVLKTLRANK 60
            MSFGLTNAPAVFMDLMNRVFKDFLDSFVIVFIDDILIYSKTEAEHE+HLHQVL+TLRANK
Sbjct: 756  MSFGLTNAPAVFMDLMNRVFKDFLDSFVIVFIDDILIYSKTEAEHEEHLHQVLETLRANK 815

Query: 61   LYAKFSKCEFLLKKVTFLSHVVSSEGVSVDPAKIEAVTSWPRPSIVSEIRSFLGLAGYYR 120
            LYAKFSKCEF L+KVTFL HVVSSEGVSVDP KIEAVT+WPRPS VSEIRSFLGLAGYYR
Sbjct: 816  LYAKFSKCEFWLRKVTFLGHVVSSEGVSVDPTKIEAVTNWPRPSTVSEIRSFLGLAGYYR 875

Query: 121  RFVEDFSRIASPLTQLTRKGTPFVWSPACESSFQELKRKLVSAPVLTVPDGSGSFMIYSD 180
            RFVEDFSRIASPLTQLTRKGTPFVWSPACESSFQELK+KLV+APVLTVPDGSG+F+IYSD
Sbjct: 876  RFVEDFSRIASPLTQLTRKGTPFVWSPACESSFQELKQKLVTAPVLTVPDGSGNFVIYSD 935

Query: 181  ASKKRLGCVLMQQGKVVAYASCQLKSHEQNYPTHDLELAAVVFALKIWRDYLYGEKIQIF 240
            ASKK LGCVLMQQGKVVAYAS QLKSHEQNYPTHDLELAAVVFALKIWR YLYGEKIQIF
Sbjct: 936  ASKKGLGCVLMQQGKVVAYASRQLKSHEQNYPTHDLELAAVVFALKIWRRYLYGEKIQIF 995

Query: 241  TDHKSMKYFFT 252
            TDHKS+KYFFT
Sbjct: 996  TDHKSLKYFFT 1006

BLAST of Cmc07g0192221 vs. NCBI nr
Match: KAA0063946.1 (pol protein [Cucumis melo var. makuwa])

HSP 1 Score: 473.0 bits (1216), Expect = 1.6e-129
Identity = 236/251 (94.02%), Postives = 244/251 (97.21%), Query Frame = 0

Query: 1    MSFGLTNAPAVFMDLMNRVFKDFLDSFVIVFIDDILIYSKTEAEHEKHLHQVLKTLRANK 60
            MSFGLTNAPAVFMDLMNRVFKDFLDSFVIVFIDDILIYSKTEAEHE+HLHQVL+TLRANK
Sbjct: 939  MSFGLTNAPAVFMDLMNRVFKDFLDSFVIVFIDDILIYSKTEAEHEEHLHQVLETLRANK 998

Query: 61   LYAKFSKCEFLLKKVTFLSHVVSSEGVSVDPAKIEAVTSWPRPSIVSEIRSFLGLAGYYR 120
            LYAKFSKCEF L+KVTFL HVVSSEGVSVDPAKIEAVT+WPRPS VSEIRSFLGLAGYYR
Sbjct: 999  LYAKFSKCEFWLRKVTFLGHVVSSEGVSVDPAKIEAVTNWPRPSTVSEIRSFLGLAGYYR 1058

Query: 121  RFVEDFSRIASPLTQLTRKGTPFVWSPACESSFQELKRKLVSAPVLTVPDGSGSFMIYSD 180
            RFVEDFSR ASPLTQLTRKGTPFVWSPACESSFQELK+KLV+APVLTVPDGSGSF+IYSD
Sbjct: 1059 RFVEDFSRKASPLTQLTRKGTPFVWSPACESSFQELKQKLVTAPVLTVPDGSGSFVIYSD 1118

Query: 181  ASKKRLGCVLMQQGKVVAYASCQLKSHEQNYPTHDLELAAVVFALKIWRDYLYGEKIQIF 240
            ASKK LGCVLMQQGKVVAYAS QLKSHEQNYPTHDLELAAVVFALKIWR YLYGEKIQIF
Sbjct: 1119 ASKKGLGCVLMQQGKVVAYASRQLKSHEQNYPTHDLELAAVVFALKIWRRYLYGEKIQIF 1178

Query: 241  TDHKSMKYFFT 252
            TDHKS+KYFFT
Sbjct: 1179 TDHKSLKYFFT 1189

BLAST of Cmc07g0192221 vs. NCBI nr
Match: TYK04344.1 (reverse transcriptase [Cucumis melo var. makuwa])

HSP 1 Score: 472.6 bits (1215), Expect = 2.1e-129
Identity = 233/251 (92.83%), Postives = 243/251 (96.81%), Query Frame = 0

Query: 1   MSFGLTNAPAVFMDLMNRVFKDFLDSFVIVFIDDILIYSKTEAEHEKHLHQVLKTLRANK 60
           MSFGLTNAPAVFMDLMNRVFKDFLDSFVIVFIDDILIYSKTEA+HE+HLHQVL+TLRANK
Sbjct: 486 MSFGLTNAPAVFMDLMNRVFKDFLDSFVIVFIDDILIYSKTEAKHEEHLHQVLETLRANK 545

Query: 61  LYAKFSKCEFLLKKVTFLSHVVSSEGVSVDPAKIEAVTSWPRPSIVSEIRSFLGLAGYYR 120
           LYAKFSKCEF LKKVTFL HVVSSEGVSVDPAKIEAVT+WPRPSIVSEIRSFLGL GYYR
Sbjct: 546 LYAKFSKCEFWLKKVTFLGHVVSSEGVSVDPAKIEAVTNWPRPSIVSEIRSFLGLTGYYR 605

Query: 121 RFVEDFSRIASPLTQLTRKGTPFVWSPACESSFQELKRKLVSAPVLTVPDGSGSFMIYSD 180
           RFVEDFSRIASPL QLTRKGTPFVWSPACESSFQELK+KLV+APVLT+PDGSGSFMIYS+
Sbjct: 606 RFVEDFSRIASPLIQLTRKGTPFVWSPACESSFQELKQKLVTAPVLTMPDGSGSFMIYSN 665

Query: 181 ASKKRLGCVLMQQGKVVAYASCQLKSHEQNYPTHDLELAAVVFALKIWRDYLYGEKIQIF 240
           ASKK LGCVLMQQGKVVAY SCQLKSHEQNYPTHDLELAAVVF LKIWR YLYGEKIQI+
Sbjct: 666 ASKKGLGCVLMQQGKVVAYTSCQLKSHEQNYPTHDLELAAVVFTLKIWRHYLYGEKIQIY 725

Query: 241 TDHKSMKYFFT 252
           TDHKS+KYFFT
Sbjct: 726 TDHKSLKYFFT 736

BLAST of Cmc07g0192221 vs. NCBI nr
Match: KAA0063098.1 (pol protein [Cucumis melo var. makuwa])

HSP 1 Score: 471.5 bits (1212), Expect = 4.6e-129
Identity = 233/251 (92.83%), Postives = 244/251 (97.21%), Query Frame = 0

Query: 1   MSFGLTNAPAVFMDLMNRVFKDFLDSFVIVFIDDILIYSKTEAEHEKHLHQVLKTLRANK 60
           MSFGLTNAPAVFMDLMNRVFKDFLDSFVIVFIDDILIYSKTEAEHE+HLHQVL+TLRANK
Sbjct: 713 MSFGLTNAPAVFMDLMNRVFKDFLDSFVIVFIDDILIYSKTEAEHEEHLHQVLETLRANK 772

Query: 61  LYAKFSKCEFLLKKVTFLSHVVSSEGVSVDPAKIEAVTSWPRPSIVSEIRSFLGLAGYYR 120
           LYAKFSKCEF L+KVTFL HVVSSEGVSVDPAKIEAVT+WPRPS VSEIRSFLGLAGYYR
Sbjct: 773 LYAKFSKCEFWLRKVTFLGHVVSSEGVSVDPAKIEAVTNWPRPSTVSEIRSFLGLAGYYR 832

Query: 121 RFVEDFSRIASPLTQLTRKGTPFVWSPACESSFQELKRKLVSAPVLTVPDGSGSFMIYSD 180
           RFVEDFSRIASPLTQLTRKGTPFVWSPACE SFQELK+KLV+APVLTVPDGSG+F+IYSD
Sbjct: 833 RFVEDFSRIASPLTQLTRKGTPFVWSPACERSFQELKQKLVTAPVLTVPDGSGNFVIYSD 892

Query: 181 ASKKRLGCVLMQQGKVVAYASCQLKSHEQNYPTHDLELAAVVFALKIWRDYLYGEKIQIF 240
           ASKK LGCVLMQQGKVVAYAS QLK+HEQNYPTHDLELAAVVFALKIWR YLYGEKIQI+
Sbjct: 893 ASKKELGCVLMQQGKVVAYASRQLKTHEQNYPTHDLELAAVVFALKIWRHYLYGEKIQIY 952

Query: 241 TDHKSMKYFFT 252
           TDHKS+KYFFT
Sbjct: 953 TDHKSLKYFFT 963

BLAST of Cmc07g0192221 vs. NCBI nr
Match: KAA0066951.1 (pol protein [Cucumis melo var. makuwa])

HSP 1 Score: 471.5 bits (1212), Expect = 4.6e-129
Identity = 234/251 (93.23%), Postives = 244/251 (97.21%), Query Frame = 0

Query: 1   MSFGLTNAPAVFMDLMNRVFKDFLDSFVIVFIDDILIYSKTEAEHEKHLHQVLKTLRANK 60
           MSFGLTNAPAVFMDLMNRVFKDFLDSFVIVFIDDILIYSKTEAEHE+HLHQVL+TLRANK
Sbjct: 521 MSFGLTNAPAVFMDLMNRVFKDFLDSFVIVFIDDILIYSKTEAEHEEHLHQVLETLRANK 580

Query: 61  LYAKFSKCEFLLKKVTFLSHVVSSEGVSVDPAKIEAVTSWPRPSIVSEIRSFLGLAGYYR 120
           LYAKFSKCEF L+KVTFL HVVSSEGVSVDPAKIEAVT+WPRPS VSEIRSFLGLAGYYR
Sbjct: 581 LYAKFSKCEFWLRKVTFLGHVVSSEGVSVDPAKIEAVTNWPRPSTVSEIRSFLGLAGYYR 640

Query: 121 RFVEDFSRIASPLTQLTRKGTPFVWSPACESSFQELKRKLVSAPVLTVPDGSGSFMIYSD 180
           RFVEDFSRIASPLTQLTRKGTPFVWSPACESSFQELK+KLV+APVLTVPDGSG+F+IYSD
Sbjct: 641 RFVEDFSRIASPLTQLTRKGTPFVWSPACESSFQELKQKLVTAPVLTVPDGSGNFVIYSD 700

Query: 181 ASKKRLGCVLMQQGKVVAYASCQLKSHEQNYPTHDLELAAVVFALKIWRDYLYGEKIQIF 240
           ASKK LGCVLMQQGKVVAYAS QLK HEQNYPTHDLELAAVVFALKIWR YLYGEKIQI+
Sbjct: 701 ASKKGLGCVLMQQGKVVAYASRQLKVHEQNYPTHDLELAAVVFALKIWRHYLYGEKIQIY 760

Query: 241 TDHKSMKYFFT 252
           TDHKS+KYFFT
Sbjct: 761 TDHKSLKYFFT 771

BLAST of Cmc07g0192221 vs. ExPASy Swiss-Prot
Match: P04323 (Retrovirus-related Pol polyprotein from transposon 17.6 OS=Drosophila melanogaster OX=7227 GN=pol PE=4 SV=1)

HSP 1 Score: 194.5 bits (493), Expect = 1.4e-48
Identity = 99/251 (39.44%), Postives = 153/251 (60.96%), Query Frame = 0

Query: 1   MSFGLTNAPAVFMDLMNRVFKDFLDSFVIVFIDDILIYSKTEAEHEKHLHQVLKTLRANK 60
           M FGL NAPA F   MN + +  L+   +V++DDI+++S +  EH + L  V + L    
Sbjct: 334 MPFGLKNAPATFQRCMNDILRPLLNKHCLVYLDDIIVFSTSLDEHLQSLGLVFEKLAKAN 393

Query: 61  LYAKFSKCEFLLKKVTFLSHVVSSEGVSVDPAKIEAVTSWPRPSIVSEIRSFLGLAGYYR 120
           L  +  KCEFL ++ TFL HV++ +G+  +P KIEA+  +P P+   EI++FLGL GYYR
Sbjct: 394 LKLQLDKCEFLKQETTFLGHVLTPDGIKPNPEKIEAIQKYPIPTKPKEIKAFLGLTGYYR 453

Query: 121 RFVEDFSRIASPLTQLTRKGTPF-VWSPACESSFQELKRKLVSAPVLTVPDGSGSFMIYS 180
           +F+ +F+ IA P+T+  +K       +P  +S+F++LK  +   P+L VPD +  F + +
Sbjct: 454 KFIPNFADIAKPMTKCLKKNMKIDTTNPEYDSAFKKLKYLISEDPILKVPDFTKKFTLTT 513

Query: 181 DASKKRLGCVLMQQGKVVAYASCQLKSHEQNYPTHDLELAAVVFALKIWRDYLYGEKIQI 240
           DAS   LG VL Q G  ++Y S  L  HE NY T + EL A+V+A K +R YL G   +I
Sbjct: 514 DASDVALGAVLSQDGHPLSYISRTLNEHEINYSTIEKELLAIVWATKTFRHYLLGRHFEI 573

Query: 241 FTDHKSMKYFF 251
            +DH+ + + +
Sbjct: 574 SSDHQPLSWLY 584

BLAST of Cmc07g0192221 vs. ExPASy Swiss-Prot
Match: P20825 (Retrovirus-related Pol polyprotein from transposon 297 OS=Drosophila melanogaster OX=7227 GN=pol PE=4 SV=1)

HSP 1 Score: 187.6 bits (475), Expect = 1.7e-46
Identity = 94/249 (37.75%), Postives = 147/249 (59.04%), Query Frame = 0

Query: 1   MSFGLTNAPAVFMDLMNRVFKDFLDSFVIVFIDDILIYSKTEAEHEKHLHQVLKTLRANK 60
           M FGL NAPA F   MN + +  L+   +V++DDI+I+S +  EH   +  V   L    
Sbjct: 333 MPFGLRNAPATFQRCMNNILRPLLNKHCLVYLDDIIIFSTSLTEHLNSIQLVFTKLADAN 392

Query: 61  LYAKFSKCEFLLKKVTFLSHVVSSEGVSVDPAKIEAVTSWPRPSIVSEIRSFLGLAGYYR 120
           L  +  KCEFL K+  FL H+V+ +G+  +P K++A+ S+P P+   EIR+FLGL GYYR
Sbjct: 393 LKLQLDKCEFLKKEANFLGHIVTPDGIKPNPIKVKAIVSYPIPTKDKEIRAFLGLTGYYR 452

Query: 121 RFVEDFSRIASPLTQLTRKGTPF-VWSPACESSFQELKRKLVSAPVLTVPDGSGSFMIYS 180
           +F+ +++ IA P+T   +K T           +F++LK  ++  P+L +PD    F++ +
Sbjct: 453 KFIPNYADIAKPMTSCLKKRTKIDTQKLEYIEAFEKLKALIIRDPILQLPDFEKKFVLTT 512

Query: 181 DASKKRLGCVLMQQGKVVAYASCQLKSHEQNYPTHDLELAAVVFALKIWRDYLYGEKIQI 240
           DAS   LG VL Q G  +++ S  L  HE NY   + EL A+V+A K +R YL G +  I
Sbjct: 513 DASNLALGAVLSQNGHPISFISRTLNDHELNYSAIEKELLAIVWATKTFRHYLLGRQFLI 572

Query: 241 FTDHKSMKY 249
            +DH+ +++
Sbjct: 573 ASDHQPLRW 581

BLAST of Cmc07g0192221 vs. ExPASy Swiss-Prot
Match: P10401 (Retrovirus-related Pol polyprotein from transposon gypsy OS=Drosophila melanogaster OX=7227 GN=pol PE=4 SV=1)

HSP 1 Score: 173.3 bits (438), Expect = 3.4e-42
Identity = 93/261 (35.63%), Postives = 150/261 (57.47%), Query Frame = 0

Query: 1   MSFGLTNAPAVFMDLMNRVFKDFLDSFVIVFIDDILIYSKTEAEHEKHLHQVLKTLRANK 60
           + FGL NA ++F   ++ V ++ +     V++DD++I+S+ E++H +H+  VLK L    
Sbjct: 309 LPFGLRNASSIFQRALDDVLREQIGKICYVYVDDVIIFSENESDHVRHIDTVLKCLIDAN 368

Query: 61  LYAKFSKCEFLLKKVTFLSHVVSSEGVSVDPAKIEAVTSWPRPSIVSEIRSFLGLAGYYR 120
           +     K  F  + V +L  +VS +G   DP K++A+  +P P  V ++RSFLGLA YYR
Sbjct: 369 MRVSQEKTRFFKESVEYLGFIVSKDGTKSDPEKVKAIQEYPEPDCVYKVRSFLGLASYYR 428

Query: 121 RFVEDFSRIASPLTQLTR-----------KGTPFVWSPACESSFQELKRKLVSAPV-LTV 180
            F++DF+ IA P+T + +           K  P  ++    ++FQ L+  L S  V L  
Sbjct: 429 VFIKDFAAIARPITDILKGENGSVSKHMSKKIPVEFNETQRNAFQRLRNILASEDVILKY 488

Query: 181 PDGSGSFMIYSDASKKRLGCVLMQQGKVVAYASCQLKSHEQNYPTHDLELAAVVFALKIW 240
           PD    F + +DAS   +G VL Q+G+ +   S  LK  EQNY T++ EL A+V+AL   
Sbjct: 489 PDFKKPFDLTTDASASGIGAVLSQEGRPITMISRTLKQPEQNYATNERELLAIVWALGKL 548

Query: 241 RDYLYGEK-IQIFTDHKSMKY 249
           +++LYG + I IFTDH+ + +
Sbjct: 549 QNFLYGSREINIFTDHQPLTF 569

BLAST of Cmc07g0192221 vs. ExPASy Swiss-Prot
Match: Q8I7P9 (Retrovirus-related Pol polyprotein from transposon opus OS=Drosophila melanogaster OX=7227 GN=pol PE=4 SV=1)

HSP 1 Score: 169.5 bits (428), Expect = 4.9e-41
Identity = 89/264 (33.71%), Postives = 149/264 (56.44%), Query Frame = 0

Query: 1   MSFGLTNAPAVFMDLMNRVFKDFLDSFVIVFIDDILIYSKTEAEHEKHLHQVLKTLRANK 60
           + FGL NAPA+F  +++ + ++ +     V+IDDI+++S+    H K+L  VL +L    
Sbjct: 250 LPFGLKNAPAIFQRMIDDILREHIGKVCYVYIDDIIVFSEDYDTHWKNLRLVLASLSKAN 309

Query: 61  LYAKFSKCEFLLKKVTFLSHVVSSEGVSVDPAKIEAVTSWPRPSIVSEIRSFLGLAGYYR 120
           L     K  FL  +V FL ++V+++G+  DP K+ A++  P P+ V E++ FLG+  YYR
Sbjct: 310 LQVNLEKSHFLDTQVEFLGYIVTADGIKADPKKVRAISEMPPPTSVKELKRFLGMTSYYR 369

Query: 121 RFVEDFSRIASPLTQLTR-----------KGTPFVWSPACESSFQELKRKLVSAPVLTVP 180
           +F++D++++A PLT LTR              P         SF +LK  L S+ +L  P
Sbjct: 370 KFIQDYAKVAKPLTNLTRGLYANIKSSQSSKVPITLDETALQSFNDLKSILCSSEILAFP 429

Query: 181 DGSGSFMIYSDASKKRLGCVLMQ----QGKVVAYASCQLKSHEQNYPTHDLELAAVVFAL 240
             +  F + +DAS   +G VL Q    + + +AY S  L   E+NY T + E+ A++++L
Sbjct: 430 CFTKPFHLTTDASNWAIGAVLSQDDQGRDRPIAYISRSLNKTEENYATIEKEMLAIIWSL 489

Query: 241 KIWRDYLYGE-KIQIFTDHKSMKY 249
              R YLYG   I+++TDH+ + +
Sbjct: 490 DNLRAYLYGAGTIKVYTDHQPLTF 513

BLAST of Cmc07g0192221 vs. ExPASy Swiss-Prot
Match: P0CT41 (Transposon Tf2-12 polyprotein OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=Tf2-12 PE=3 SV=1)

HSP 1 Score: 159.8 bits (403), Expect = 3.9e-38
Identity = 87/253 (34.39%), Postives = 140/253 (55.34%), Query Frame = 0

Query: 1   MSFGLTNAPAVFMDLMNRVFKDFLDSFVIVFIDDILIYSKTEAEHEKHLHQVLKTLRANK 60
           M +G++ APA F   +N +  +  +S V+ ++DDILI+SK+E+EH KH+  VL+ L+   
Sbjct: 534 MPYGISTAPAHFQYFINTILGEAKESHVVCYMDDILIHSKSESEHVKHVKDVLQKLKNAN 593

Query: 61  LYAKFSKCEFLLKKVTFLSHVVSSEGVSVDPAKIEAVTSWPRPSIVSEIRSFLGLAGYYR 120
           L    +KCEF   +V F+ + +S +G +     I+ V  W +P    E+R FLG   Y R
Sbjct: 594 LIINQAKCEFHQSQVKFIGYHISEKGFTPCQENIDKVLQWKQPKNRKELRQFLGSVNYLR 653

Query: 121 RFVEDFSRIASPLTQLTRKGTPFVWSPACESSFQELKRKLVSAPVLTVPDGSGSFMIYSD 180
           +F+   S++  PL  L +K   + W+P    + + +K+ LVS PVL   D S   ++ +D
Sbjct: 654 KFIPKTSQLTHPLNNLLKKDVRWKWTPTQTQAIENIKQCLVSPPVLRHFDFSKKILLETD 713

Query: 181 ASKKRLGCVLMQQGK-----VVAYASCQLKSHEQNYPTHDLELAAVVFALKIWRDYLYG- 240
           AS   +G VL Q+        V Y S ++   + NY   D E+ A++ +LK WR YL   
Sbjct: 714 ASDVAVGAVLSQKHDDDKYYPVGYYSAKMSKAQLNYSVSDKEMLAIIKSLKHWRHYLEST 773

Query: 241 -EKIQIFTDHKSM 247
            E  +I TDH+++
Sbjct: 774 IEPFKILTDHRNL 786

BLAST of Cmc07g0192221 vs. ExPASy TrEMBL
Match: A0A5A7VBY3 (Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold616G00920 PE=4 SV=1)

HSP 1 Score: 473.0 bits (1216), Expect = 7.7e-130
Identity = 236/251 (94.02%), Postives = 244/251 (97.21%), Query Frame = 0

Query: 1    MSFGLTNAPAVFMDLMNRVFKDFLDSFVIVFIDDILIYSKTEAEHEKHLHQVLKTLRANK 60
            MSFGLTNAPAVFMDLMNRVFKDFLDSFVIVFIDDILIYSKTEAEHE+HLHQVL+TLRANK
Sbjct: 939  MSFGLTNAPAVFMDLMNRVFKDFLDSFVIVFIDDILIYSKTEAEHEEHLHQVLETLRANK 998

Query: 61   LYAKFSKCEFLLKKVTFLSHVVSSEGVSVDPAKIEAVTSWPRPSIVSEIRSFLGLAGYYR 120
            LYAKFSKCEF L+KVTFL HVVSSEGVSVDPAKIEAVT+WPRPS VSEIRSFLGLAGYYR
Sbjct: 999  LYAKFSKCEFWLRKVTFLGHVVSSEGVSVDPAKIEAVTNWPRPSTVSEIRSFLGLAGYYR 1058

Query: 121  RFVEDFSRIASPLTQLTRKGTPFVWSPACESSFQELKRKLVSAPVLTVPDGSGSFMIYSD 180
            RFVEDFSR ASPLTQLTRKGTPFVWSPACESSFQELK+KLV+APVLTVPDGSGSF+IYSD
Sbjct: 1059 RFVEDFSRKASPLTQLTRKGTPFVWSPACESSFQELKQKLVTAPVLTVPDGSGSFVIYSD 1118

Query: 181  ASKKRLGCVLMQQGKVVAYASCQLKSHEQNYPTHDLELAAVVFALKIWRDYLYGEKIQIF 240
            ASKK LGCVLMQQGKVVAYAS QLKSHEQNYPTHDLELAAVVFALKIWR YLYGEKIQIF
Sbjct: 1119 ASKKGLGCVLMQQGKVVAYASRQLKSHEQNYPTHDLELAAVVFALKIWRRYLYGEKIQIF 1178

Query: 241  TDHKSMKYFFT 252
            TDHKS+KYFFT
Sbjct: 1179 TDHKSLKYFFT 1189

BLAST of Cmc07g0192221 vs. ExPASy TrEMBL
Match: A0A5D3BZN1 (Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1415G00670 PE=4 SV=1)

HSP 1 Score: 473.0 bits (1216), Expect = 7.7e-130
Identity = 235/251 (93.63%), Postives = 244/251 (97.21%), Query Frame = 0

Query: 1    MSFGLTNAPAVFMDLMNRVFKDFLDSFVIVFIDDILIYSKTEAEHEKHLHQVLKTLRANK 60
            MSFGLTNAPAVFMDLMNRVFKDFLDSFVIVFIDDILIYSKTEAEHE+HLHQVL+TLRANK
Sbjct: 756  MSFGLTNAPAVFMDLMNRVFKDFLDSFVIVFIDDILIYSKTEAEHEEHLHQVLETLRANK 815

Query: 61   LYAKFSKCEFLLKKVTFLSHVVSSEGVSVDPAKIEAVTSWPRPSIVSEIRSFLGLAGYYR 120
            LYAKFSKCEF L+KVTFL HVVSSEGVSVDP KIEAVT+WPRPS VSEIRSFLGLAGYYR
Sbjct: 816  LYAKFSKCEFWLRKVTFLGHVVSSEGVSVDPTKIEAVTNWPRPSTVSEIRSFLGLAGYYR 875

Query: 121  RFVEDFSRIASPLTQLTRKGTPFVWSPACESSFQELKRKLVSAPVLTVPDGSGSFMIYSD 180
            RFVEDFSRIASPLTQLTRKGTPFVWSPACESSFQELK+KLV+APVLTVPDGSG+F+IYSD
Sbjct: 876  RFVEDFSRIASPLTQLTRKGTPFVWSPACESSFQELKQKLVTAPVLTVPDGSGNFVIYSD 935

Query: 181  ASKKRLGCVLMQQGKVVAYASCQLKSHEQNYPTHDLELAAVVFALKIWRDYLYGEKIQIF 240
            ASKK LGCVLMQQGKVVAYAS QLKSHEQNYPTHDLELAAVVFALKIWR YLYGEKIQIF
Sbjct: 936  ASKKGLGCVLMQQGKVVAYASRQLKSHEQNYPTHDLELAAVVFALKIWRRYLYGEKIQIF 995

Query: 241  TDHKSMKYFFT 252
            TDHKS+KYFFT
Sbjct: 996  TDHKSLKYFFT 1006

BLAST of Cmc07g0192221 vs. ExPASy TrEMBL
Match: A0A5D3BZD2 (Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold675G00070 PE=4 SV=1)

HSP 1 Score: 472.6 bits (1215), Expect = 1.0e-129
Identity = 233/251 (92.83%), Postives = 243/251 (96.81%), Query Frame = 0

Query: 1   MSFGLTNAPAVFMDLMNRVFKDFLDSFVIVFIDDILIYSKTEAEHEKHLHQVLKTLRANK 60
           MSFGLTNAPAVFMDLMNRVFKDFLDSFVIVFIDDILIYSKTEA+HE+HLHQVL+TLRANK
Sbjct: 486 MSFGLTNAPAVFMDLMNRVFKDFLDSFVIVFIDDILIYSKTEAKHEEHLHQVLETLRANK 545

Query: 61  LYAKFSKCEFLLKKVTFLSHVVSSEGVSVDPAKIEAVTSWPRPSIVSEIRSFLGLAGYYR 120
           LYAKFSKCEF LKKVTFL HVVSSEGVSVDPAKIEAVT+WPRPSIVSEIRSFLGL GYYR
Sbjct: 546 LYAKFSKCEFWLKKVTFLGHVVSSEGVSVDPAKIEAVTNWPRPSIVSEIRSFLGLTGYYR 605

Query: 121 RFVEDFSRIASPLTQLTRKGTPFVWSPACESSFQELKRKLVSAPVLTVPDGSGSFMIYSD 180
           RFVEDFSRIASPL QLTRKGTPFVWSPACESSFQELK+KLV+APVLT+PDGSGSFMIYS+
Sbjct: 606 RFVEDFSRIASPLIQLTRKGTPFVWSPACESSFQELKQKLVTAPVLTMPDGSGSFMIYSN 665

Query: 181 ASKKRLGCVLMQQGKVVAYASCQLKSHEQNYPTHDLELAAVVFALKIWRDYLYGEKIQIF 240
           ASKK LGCVLMQQGKVVAY SCQLKSHEQNYPTHDLELAAVVF LKIWR YLYGEKIQI+
Sbjct: 666 ASKKGLGCVLMQQGKVVAYTSCQLKSHEQNYPTHDLELAAVVFTLKIWRHYLYGEKIQIY 725

Query: 241 TDHKSMKYFFT 252
           TDHKS+KYFFT
Sbjct: 726 TDHKSLKYFFT 736

BLAST of Cmc07g0192221 vs. ExPASy TrEMBL
Match: A0A5A7VMR4 (Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold550G00890 PE=4 SV=1)

HSP 1 Score: 471.5 bits (1212), Expect = 2.2e-129
Identity = 234/251 (93.23%), Postives = 244/251 (97.21%), Query Frame = 0

Query: 1   MSFGLTNAPAVFMDLMNRVFKDFLDSFVIVFIDDILIYSKTEAEHEKHLHQVLKTLRANK 60
           MSFGLTNAPAVFMDLMNRVFKDFLDSFVIVFIDDILIYSKTEAEHE+HLHQVL+TLRANK
Sbjct: 521 MSFGLTNAPAVFMDLMNRVFKDFLDSFVIVFIDDILIYSKTEAEHEEHLHQVLETLRANK 580

Query: 61  LYAKFSKCEFLLKKVTFLSHVVSSEGVSVDPAKIEAVTSWPRPSIVSEIRSFLGLAGYYR 120
           LYAKFSKCEF L+KVTFL HVVSSEGVSVDPAKIEAVT+WPRPS VSEIRSFLGLAGYYR
Sbjct: 581 LYAKFSKCEFWLRKVTFLGHVVSSEGVSVDPAKIEAVTNWPRPSTVSEIRSFLGLAGYYR 640

Query: 121 RFVEDFSRIASPLTQLTRKGTPFVWSPACESSFQELKRKLVSAPVLTVPDGSGSFMIYSD 180
           RFVEDFSRIASPLTQLTRKGTPFVWSPACESSFQELK+KLV+APVLTVPDGSG+F+IYSD
Sbjct: 641 RFVEDFSRIASPLTQLTRKGTPFVWSPACESSFQELKQKLVTAPVLTVPDGSGNFVIYSD 700

Query: 181 ASKKRLGCVLMQQGKVVAYASCQLKSHEQNYPTHDLELAAVVFALKIWRDYLYGEKIQIF 240
           ASKK LGCVLMQQGKVVAYAS QLK HEQNYPTHDLELAAVVFALKIWR YLYGEKIQI+
Sbjct: 701 ASKKGLGCVLMQQGKVVAYASRQLKVHEQNYPTHDLELAAVVFALKIWRHYLYGEKIQIY 760

Query: 241 TDHKSMKYFFT 252
           TDHKS+KYFFT
Sbjct: 761 TDHKSLKYFFT 771

BLAST of Cmc07g0192221 vs. ExPASy TrEMBL
Match: A0A5A7V646 (Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold1337G00030 PE=4 SV=1)

HSP 1 Score: 471.5 bits (1212), Expect = 2.2e-129
Identity = 233/251 (92.83%), Postives = 244/251 (97.21%), Query Frame = 0

Query: 1   MSFGLTNAPAVFMDLMNRVFKDFLDSFVIVFIDDILIYSKTEAEHEKHLHQVLKTLRANK 60
           MSFGLTNAPAVFMDLMNRVFKDFLDSFVIVFIDDILIYSKTEAEHE+HLHQVL+TLRANK
Sbjct: 713 MSFGLTNAPAVFMDLMNRVFKDFLDSFVIVFIDDILIYSKTEAEHEEHLHQVLETLRANK 772

Query: 61  LYAKFSKCEFLLKKVTFLSHVVSSEGVSVDPAKIEAVTSWPRPSIVSEIRSFLGLAGYYR 120
           LYAKFSKCEF L+KVTFL HVVSSEGVSVDPAKIEAVT+WPRPS VSEIRSFLGLAGYYR
Sbjct: 773 LYAKFSKCEFWLRKVTFLGHVVSSEGVSVDPAKIEAVTNWPRPSTVSEIRSFLGLAGYYR 832

Query: 121 RFVEDFSRIASPLTQLTRKGTPFVWSPACESSFQELKRKLVSAPVLTVPDGSGSFMIYSD 180
           RFVEDFSRIASPLTQLTRKGTPFVWSPACE SFQELK+KLV+APVLTVPDGSG+F+IYSD
Sbjct: 833 RFVEDFSRIASPLTQLTRKGTPFVWSPACERSFQELKQKLVTAPVLTVPDGSGNFVIYSD 892

Query: 181 ASKKRLGCVLMQQGKVVAYASCQLKSHEQNYPTHDLELAAVVFALKIWRDYLYGEKIQIF 240
           ASKK LGCVLMQQGKVVAYAS QLK+HEQNYPTHDLELAAVVFALKIWR YLYGEKIQI+
Sbjct: 893 ASKKELGCVLMQQGKVVAYASRQLKTHEQNYPTHDLELAAVVFALKIWRHYLYGEKIQIY 952

Query: 241 TDHKSMKYFFT 252
           TDHKS+KYFFT
Sbjct: 953 TDHKSLKYFFT 963

BLAST of Cmc07g0192221 vs. TAIR 10
Match: ATMG00860.1 (DNA/RNA polymerases superfamily protein )

HSP 1 Score: 110.9 bits (276), Expect = 1.5e-24
Identity = 53/125 (42.40%), Postives = 78/125 (62.40%), Query Frame = 0

Query: 48  HLHQVLKTLRANKLYAKFSKCEFLLKKVTFLS--HVVSSEGVSVDPAKIEAVTSWPRPSI 107
           HL  VL+    ++ YA   KC F   ++ +L   H++S EGVS DPAK+EA+  WP P  
Sbjct: 3   HLGMVLQIWEQHQFYANRKKCAFGQPQIAYLGHRHIISGEGVSADPAKLEAMVGWPEPKN 62

Query: 108 VSEIRSFLGLAGYYRRFVEDFSRIASPLTQLTRKGTPFVWSPACESSFQELKRKLVSAPV 167
            +E+R FLGL GYYRRFV+++ +I  PLT+L +K +   W+     +F+ LK  + + PV
Sbjct: 63  TTELRGFLGLTGYYRRFVKNYGKIVRPLTELLKKNS-LKWTEMAALAFKALKGAVTTLPV 122

Query: 168 LTVPD 171
           L +PD
Sbjct: 123 LALPD 126

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
TYK05193.11.6e-12993.63pol protein [Cucumis melo var. makuwa][more]
KAA0063946.11.6e-12994.02pol protein [Cucumis melo var. makuwa][more]
TYK04344.12.1e-12992.83reverse transcriptase [Cucumis melo var. makuwa][more]
KAA0063098.14.6e-12992.83pol protein [Cucumis melo var. makuwa][more]
KAA0066951.14.6e-12993.23pol protein [Cucumis melo var. makuwa][more]
Match NameE-valueIdentityDescription
P043231.4e-4839.44Retrovirus-related Pol polyprotein from transposon 17.6 OS=Drosophila melanogast... [more]
P208251.7e-4637.75Retrovirus-related Pol polyprotein from transposon 297 OS=Drosophila melanogaste... [more]
P104013.4e-4235.63Retrovirus-related Pol polyprotein from transposon gypsy OS=Drosophila melanogas... [more]
Q8I7P94.9e-4133.71Retrovirus-related Pol polyprotein from transposon opus OS=Drosophila melanogast... [more]
P0CT413.9e-3834.39Transposon Tf2-12 polyprotein OS=Schizosaccharomyces pombe (strain 972 / ATCC 24... [more]
Match NameE-valueIdentityDescription
A0A5A7VBY37.7e-13094.02Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold61... [more]
A0A5D3BZN17.7e-13093.63Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold14... [more]
A0A5D3BZD21.0e-12992.83Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold67... [more]
A0A5A7VMR42.2e-12993.23Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold55... [more]
A0A5A7V6462.2e-12992.83Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold13... [more]
Match NameE-valueIdentityDescription
ATMG00860.11.5e-2442.40DNA/RNA polymerases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Melon (Charmono) v1.1
Date Performed: 2022-10-13
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000477Reverse transcriptase domainPFAMPF00078RVT_1coord: 1..80
e-value: 9.1E-15
score: 54.8
IPR043128Reverse transcriptase/Diguanylate cyclase domainGENE3D3.30.70.270coord: 92..185
e-value: 1.5E-31
score: 110.2
IPR043128Reverse transcriptase/Diguanylate cyclase domainGENE3D3.30.70.270coord: 1..87
e-value: 1.3E-32
score: 114.5
IPR041577Reverse transcriptase/retrotransposon-derived protein, RNase H-like domainPFAMPF17919RT_RNaseH_2coord: 145..239
e-value: 2.9E-30
score: 104.2
NoneNo IPR availablePANTHERPTHR24559:SF355PROTEIN, PUTATIVE-RELATEDcoord: 91..248
NoneNo IPR availablePANTHERPTHR24559TRANSPOSON TY3-I GAG-POL POLYPROTEINcoord: 91..248
NoneNo IPR availableCDDcd01647RT_LTRcoord: 1..82
e-value: 1.52391E-36
score: 125.4
NoneNo IPR availableCDDcd09274RNase_HI_RT_Ty3coord: 177..251
e-value: 8.97382E-32
score: 111.43
IPR043502DNA/RNA polymerase superfamilySUPERFAMILY56672DNA/RNA polymerasescoord: 1..251

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cmc07g0192221.1Cmc07g0192221.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0015074 DNA integration
biological_process GO:0006508 proteolysis
biological_process GO:0006278 RNA-dependent DNA biosynthetic process
molecular_function GO:0004190 aspartic-type endopeptidase activity
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0003964 RNA-directed DNA polymerase activity
molecular_function GO:0008270 zinc ion binding