Cmc07g0184481 (gene) Melon (Charmono) v1.1

Overview
NameCmc07g0184481
Typegene
OrganismCucumis melo L. var. cantalupensis cv. Charmono (Melon (Charmono) v1.1)
DescriptionThylakoidal processing peptidase 1
LocationCMiso1.1chr07: 2554461 .. 2557264 (-)
RNA-Seq ExpressionCmc07g0184481
SyntenyCmc07g0184481
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CTAAATTCGTTAATGAGCATCTTCTTTATAACCTATAGACGCAATGCGTATGGTGAAATTTTCTTTCTCCTACAACGAGAATCTTCCCGCCGTCGCTTCGAGAGAGATCGCCGGGAGAAATTACGCCGTTCCTTAGATCCGATCACTGGCTCTTACTATAGACGTTGCTTATGCCTTTTCAAGACGCTTATTGTTTCGAATTTTCTCTCTCGATTTAGCGGTGTTTTTTTATTCTTCTTCTATGTCGCTGGCTCAGCTTGATTTTTCTGCAGAAATGGCGGTCGTGGGCTTATAGTTATTCGTTTTTATACGTTGCTAGTCAGTGAGGGCTCTCTGATTTTTTAGAGGTTTGTTGGGGGTTTTCAGATTGTAGATATGGCTATCAGGGTTACCGTCTCCTTCTCGGGTTACGTCGCTCAAAATCTTGCATCCTCTGCCGGAATTCGTGTCGGTAATTGTCGCGCTGTTCATGAATGCTGGATTCGATCTCGACTTTTTGGCTCAAATCAGAAGCCGGAATTTGATCCTGCTGGTTCGGTGCGCAATTATCATTCCGACGTTCTTCCGTCTAATTCGAGATGTTGGGTGAAGAATTCGGCATCTGCGCTTGGCACTATCGCCGGAGAAATTGTTGATGAAAGCTGTAGAAACCCAATTGTTCTAGGTTTGATTTCGCTTATGAAATCTGCTGTAGGTACTTCCGTTTCCTCGCCAATGGCCATGGGCGTATTTGGTGTTTCTTCCTTTGAAGCATCTTCGATTATCCCATTTTTACAGGGGTCGAAGACGGTTACCGGTAATGAATCGATTTCGGGCTCAGCGGGCGATGAAATTGAAAGTTATGGAGTTTTTGACTGTGTAATGGACGAGGGCATGAGCCAGCCGCCCAACCCTTCGCAGTTAGAGAAGAGTAGCTGGATATCACGGTTCTTGAATAACTGTTCTGAGGATGCCAAGGCCATTGCTACAGCTTTGACTGTTAGTGTGCTTTTTCGCTCCTCCTTGGCTGAGCCAAGATCTATACCTTCTTCATCAATGTATCCAACCCTTGATGTGGGCGATCGCATCTTGGCTGAAAAGGTCAGACAACGTTCTTTTACTGAAATTTTAGTTTTAAATATCTGATAGAACTAGAAGAGAATGTTCGTTAGACTATTGGGTAGGTAATTGTATGTTTGGATTTATATGGAAATGTTCAAATTACTATACAAAATTTGTTTCTCGTATCTAATGTTCAATCGCCTTGAAATTTAGCCATTATTTTCTTAGAATGATGAGCTTGTTTTAACTGTTATATGTTGAGTAACCATGGAGAAATGTGGTAGAAGTGTTTGATGGTGTTCATTGTGAGACAGAGTTTACTTTATTTTATGTCTCTTGTATAGACCCTGCTTCCTTAAAGACTTGAATTTCCTTTTTCAGTTGTCAAATGATATCTAGAGATGATAACTGAAGGAAGTGTTTGATGATTGTTGAAGAAGGATAAGTAATATCAAATATAATAAAGAACTGGTTTGAAATTATTATTGATCACTTGTTTCTTTGTTAACTTGTTCAATCTTATGATTTTCAGGTGTCATATTTTTTCAGAAAGCCGAGTGTTTCTGATATTGTCATTTTCAAGGCACCTCCAATCTTGCAGGTAGCGTCTACAATCTCTAATGTTAGTGAACGTTTTCCTCGTCCCAAAGTTTAATCAAGTTGCTTATCTCATTGAACATTCTGGTACATTTGCAGAAAATTGGTTACAAGTCAAATGACGTCTTCATCAAGAGAATTGTGGCGAAGGCTGGTGATTGTGTTGAGGTTAGATATCTTCATATGTTTGAATGATTTCCCTGGGTCTCGATTCTTCGTTCATATCGTCCCTTTTGACTACAAATCAAATTATGATGGAATTTTCCGACTCCCTAAGCTCATTTTGTTTTATTACTACAGGTACGAGATGGAAAACTCTTGGTGAATGGTGTTGCTCAAAACGAGAAGTTCATCTTAGAGCCCCTTTCTTATAACATGGATCCAGTGGTAATATACCCTGCCCCTCTACTGATTAAACACACATTTAATTTGGGAGAGCATGTCCATCAAGAAATTGTTGAATGTTATGTGTTGTATTGCAGCTTGTACCCGAAGGGTATGTTTTTGTACTGGGGGACAATCGCAACAACAGTTTTGATTCTCATAACTGGTAATTTGACTATCTCGGATTTGATTTGCCTCTATTTTCTCAGCAGATCATGTTATCATTTATACTCAGGCAAACCATTTGTTCTCATCGGGTCTGTTGTCGAATTTGTTTCTTTATTGCACACAAGTTTCTACAGAATAATCGTCAGTATTGAATTGTGCTCTCATTTTGCATTTCCAGGGGTCCACTTCCTGTTGAAAACATCGTCGGTAGATCAGTGTTCCGATACTGGCCACCGTCAAAAGTTTCTGATATCATGAGTGATCAAAATGCAAAGAAAGATGTGGTAGTTTCTTGATGTTTCACCATCTAATTTTTTTGCAGAGATAGAATCTGTTCCATTTTGATATTGATTCTCAAATTACACATTCAATTTCTTTTTTGGCCATTAGATGGGAATTAGATTCATATGTAAATTCTCTGTGGTTGATTAAGCGCCACCGTTGTATTTTGATTTCATTTGATGACAAAACAAACCATTCACGTTTTAGCCTGGGAAAAAGATTCATATAAAGATGAGAGCATTTGTTCATTGTTTTCTCGAGTTTCATTTTCCCAGTTTGTATATAATTCTGAATCACAACGTTGGTTTCATGTATATATTGCAAAATGTTCT

mRNA sequence

CTAAATTCGTTAATGAGCATCTTCTTTATAACCTATAGACGCAATGCGTATGGTGAAATTTTCTTTCTCCTACAACGAGAATCTTCCCGCCGTCGCTTCGAGAGAGATCGCCGGGAGAAATTACGCCGTTCCTTAGATCCGATCACTGGCTCTTACTATAGACGTTGCTTATGCCTTTTCAAGACGCTTATTGTTTCGAATTTTCTCTCTCGATTTAGCGGTGTTTTTTTATTCTTCTTCTATGTCGCTGGCTCAGCTTGATTTTTCTGCAGAAATGGCGGTCGTGGGCTTATAGTTATTCGTTTTTATACGTTGCTAGTCAGTGAGGGCTCTCTGATTTTTTAGAGGTTTGTTGGGGGTTTTCAGATTGTAGATATGGCTATCAGGGTTACCGTCTCCTTCTCGGGTTACGTCGCTCAAAATCTTGCATCCTCTGCCGGAATTCGTGTCGGTAATTGTCGCGCTGTTCATGAATGCTGGATTCGATCTCGACTTTTTGGCTCAAATCAGAAGCCGGAATTTGATCCTGCTGGTTCGGTGCGCAATTATCATTCCGACGTTCTTCCGTCTAATTCGAGATGTTGGGTGAAGAATTCGGCATCTGCGCTTGGCACTATCGCCGGAGAAATTGTTGATGAAAGCTGTAGAAACCCAATTGTTCTAGGTTTGATTTCGCTTATGAAATCTGCTGTAGGTACTTCCGTTTCCTCGCCAATGGCCATGGGCGTATTTGGTGTTTCTTCCTTTGAAGCATCTTCGATTATCCCATTTTTACAGGGGTCGAAGACGGTTACCGGTAATGAATCGATTTCGGGCTCAGCGGGCGATGAAATTGAAAGTTATGGAGTTTTTGACTGTGTAATGGACGAGGGCATGAGCCAGCCGCCCAACCCTTCGCAGTTAGAGAAGAGTAGCTGGATATCACGGTTCTTGAATAACTGTTCTGAGGATGCCAAGGCCATTGCTACAGCTTTGACTGTTAGTGTGCTTTTTCGCTCCTCCTTGGCTGAGCCAAGATCTATACCTTCTTCATCAATGTATCCAACCCTTGATGTGGGCGATCGCATCTTGGCTGAAAAGGTGTCATATTTTTTCAGAAAGCCGAGTGTTTCTGATATTGTCATTTTCAAGGCACCTCCAATCTTGCAGAAAATTGGTTACAAGTCAAATGACGTCTTCATCAAGAGAATTGTGGCGAAGGCTGGTGATTGTGTTGAGGTACGAGATGGAAAACTCTTGGTGAATGGTGTTGCTCAAAACGAGAAGTTCATCTTAGAGCCCCTTTCTTATAACATGGATCCAGTGCTTGTACCCGAAGGGTATGTTTTTGTACTGGGGGACAATCGCAACAACAGTTTTGATTCTCATAACTGGGGTCCACTTCCTGTTGAAAACATCGTCGGTAGATCAGTGTTCCGATACTGGCCACCGTCAAAAGTTTCTGATATCATGAGTGATCAAAATGCAAAGAAAGATGTGGTAGTTTCTTGATGTTTCACCATCTAATTTTTTTGCAGAGATAGAATCTGTTCCATTTTGATATTGATTCTCAAATTACACATTCAATTTCTTTTTTGGCCATTAGATGGGAATTAGATTCATATGTAAATTCTCTGTGGTTGATTAAGCGCCACCGTTGTATTTTGATTTCATTTGATGACAAAACAAACCATTCACGTTTTAGCCTGGGAAAAAGATTCATATAAAGATGAGAGCATTTGTTCATTGTTTTCTCGAGTTTCATTTTCCCAGTTTGTATATAATTCTGAATCACAACGTTGGTTTCATGTATATATTGCAAAATGTTCT

Coding sequence (CDS)

ATGGCTATCAGGGTTACCGTCTCCTTCTCGGGTTACGTCGCTCAAAATCTTGCATCCTCTGCCGGAATTCGTGTCGGTAATTGTCGCGCTGTTCATGAATGCTGGATTCGATCTCGACTTTTTGGCTCAAATCAGAAGCCGGAATTTGATCCTGCTGGTTCGGTGCGCAATTATCATTCCGACGTTCTTCCGTCTAATTCGAGATGTTGGGTGAAGAATTCGGCATCTGCGCTTGGCACTATCGCCGGAGAAATTGTTGATGAAAGCTGTAGAAACCCAATTGTTCTAGGTTTGATTTCGCTTATGAAATCTGCTGTAGGTACTTCCGTTTCCTCGCCAATGGCCATGGGCGTATTTGGTGTTTCTTCCTTTGAAGCATCTTCGATTATCCCATTTTTACAGGGGTCGAAGACGGTTACCGGTAATGAATCGATTTCGGGCTCAGCGGGCGATGAAATTGAAAGTTATGGAGTTTTTGACTGTGTAATGGACGAGGGCATGAGCCAGCCGCCCAACCCTTCGCAGTTAGAGAAGAGTAGCTGGATATCACGGTTCTTGAATAACTGTTCTGAGGATGCCAAGGCCATTGCTACAGCTTTGACTGTTAGTGTGCTTTTTCGCTCCTCCTTGGCTGAGCCAAGATCTATACCTTCTTCATCAATGTATCCAACCCTTGATGTGGGCGATCGCATCTTGGCTGAAAAGGTGTCATATTTTTTCAGAAAGCCGAGTGTTTCTGATATTGTCATTTTCAAGGCACCTCCAATCTTGCAGAAAATTGGTTACAAGTCAAATGACGTCTTCATCAAGAGAATTGTGGCGAAGGCTGGTGATTGTGTTGAGGTACGAGATGGAAAACTCTTGGTGAATGGTGTTGCTCAAAACGAGAAGTTCATCTTAGAGCCCCTTTCTTATAACATGGATCCAGTGCTTGTACCCGAAGGGTATGTTTTTGTACTGGGGGACAATCGCAACAACAGTTTTGATTCTCATAACTGGGGTCCACTTCCTGTTGAAAACATCGTCGGTAGATCAGTGTTCCGATACTGGCCACCGTCAAAAGTTTCTGATATCATGAGTGATCAAAATGCAAAGAAAGATGTGGTAGTTTCTTGA

Protein sequence

MAIRVTVSFSGYVAQNLASSAGIRVGNCRAVHECWIRSRLFGSNQKPEFDPAGSVRNYHSDVLPSNSRCWVKNSASALGTIAGEIVDESCRNPIVLGLISLMKSAVGTSVSSPMAMGVFGVSSFEASSIIPFLQGSKTVTGNESISGSAGDEIESYGVFDCVMDEGMSQPPNPSQLEKSSWISRFLNNCSEDAKAIATALTVSVLFRSSLAEPRSIPSSSMYPTLDVGDRILAEKVSYFFRKPSVSDIVIFKAPPILQKIGYKSNDVFIKRIVAKAGDCVEVRDGKLLVNGVAQNEKFILEPLSYNMDPVLVPEGYVFVLGDNRNNSFDSHNWGPLPVENIVGRSVFRYWPPSKVSDIMSDQNAKKDVVVS
Homology
BLAST of Cmc07g0184481 vs. NCBI nr
Match: XP_008452489.1 (PREDICTED: thylakoidal processing peptidase 1, chloroplastic-like [Cucumis melo] >KAA0064352.1 thylakoidal processing peptidase 1 [Cucumis melo var. makuwa] >TYK20235.1 thylakoidal processing peptidase 1 [Cucumis melo var. makuwa])

HSP 1 Score: 736.5 bits (1900), Expect = 1.1e-208
Identity = 371/371 (100.00%), Postives = 371/371 (100.00%), Query Frame = 0

Query: 1   MAIRVTVSFSGYVAQNLASSAGIRVGNCRAVHECWIRSRLFGSNQKPEFDPAGSVRNYHS 60
           MAIRVTVSFSGYVAQNLASSAGIRVGNCRAVHECWIRSRLFGSNQKPEFDPAGSVRNYHS
Sbjct: 1   MAIRVTVSFSGYVAQNLASSAGIRVGNCRAVHECWIRSRLFGSNQKPEFDPAGSVRNYHS 60

Query: 61  DVLPSNSRCWVKNSASALGTIAGEIVDESCRNPIVLGLISLMKSAVGTSVSSPMAMGVFG 120
           DVLPSNSRCWVKNSASALGTIAGEIVDESCRNPIVLGLISLMKSAVGTSVSSPMAMGVFG
Sbjct: 61  DVLPSNSRCWVKNSASALGTIAGEIVDESCRNPIVLGLISLMKSAVGTSVSSPMAMGVFG 120

Query: 121 VSSFEASSIIPFLQGSKTVTGNESISGSAGDEIESYGVFDCVMDEGMSQPPNPSQLEKSS 180
           VSSFEASSIIPFLQGSKTVTGNESISGSAGDEIESYGVFDCVMDEGMSQPPNPSQLEKSS
Sbjct: 121 VSSFEASSIIPFLQGSKTVTGNESISGSAGDEIESYGVFDCVMDEGMSQPPNPSQLEKSS 180

Query: 181 WISRFLNNCSEDAKAIATALTVSVLFRSSLAEPRSIPSSSMYPTLDVGDRILAEKVSYFF 240
           WISRFLNNCSEDAKAIATALTVSVLFRSSLAEPRSIPSSSMYPTLDVGDRILAEKVSYFF
Sbjct: 181 WISRFLNNCSEDAKAIATALTVSVLFRSSLAEPRSIPSSSMYPTLDVGDRILAEKVSYFF 240

Query: 241 RKPSVSDIVIFKAPPILQKIGYKSNDVFIKRIVAKAGDCVEVRDGKLLVNGVAQNEKFIL 300
           RKPSVSDIVIFKAPPILQKIGYKSNDVFIKRIVAKAGDCVEVRDGKLLVNGVAQNEKFIL
Sbjct: 241 RKPSVSDIVIFKAPPILQKIGYKSNDVFIKRIVAKAGDCVEVRDGKLLVNGVAQNEKFIL 300

Query: 301 EPLSYNMDPVLVPEGYVFVLGDNRNNSFDSHNWGPLPVENIVGRSVFRYWPPSKVSDIMS 360
           EPLSYNMDPVLVPEGYVFVLGDNRNNSFDSHNWGPLPVENIVGRSVFRYWPPSKVSDIMS
Sbjct: 301 EPLSYNMDPVLVPEGYVFVLGDNRNNSFDSHNWGPLPVENIVGRSVFRYWPPSKVSDIMS 360

Query: 361 DQNAKKDVVVS 372
           DQNAKKDVVVS
Sbjct: 361 DQNAKKDVVVS 371

BLAST of Cmc07g0184481 vs. NCBI nr
Match: XP_011654082.1 (thylakoidal processing peptidase 1, chloroplastic [Cucumis sativus] >KGN55154.1 hypothetical protein Csa_012649 [Cucumis sativus])

HSP 1 Score: 712.6 bits (1838), Expect = 1.8e-201
Identity = 359/371 (96.77%), Postives = 366/371 (98.65%), Query Frame = 0

Query: 1   MAIRVTVSFSGYVAQNLASSAGIRVGNCRAVHECWIRSRLFGSNQKPEFDPAGSVRNYHS 60
           MAIRVTVSFSGYVAQNLASSAGIRVGNCRAVHECWIRSRLFGSNQKPEFDP+GSVRNYHS
Sbjct: 1   MAIRVTVSFSGYVAQNLASSAGIRVGNCRAVHECWIRSRLFGSNQKPEFDPSGSVRNYHS 60

Query: 61  DVLPSNSRCWVKNSASALGTIAGEIVDESCRNPIVLGLISLMKSAVGTSVSSPMAMGVFG 120
            VLPSNSRCWVKNSASALGTIAGEIVDESCRNPIVLGLISLMKSAVGTSVSSPMAMGVFG
Sbjct: 61  AVLPSNSRCWVKNSASALGTIAGEIVDESCRNPIVLGLISLMKSAVGTSVSSPMAMGVFG 120

Query: 121 VSSFEASSIIPFLQGSKTVTGNESISGSAGDEIESYGVFDCVMDEGMSQPPNPSQLEKSS 180
           VSSFEASSIIPFLQGSKTVTGNES+SGS GDEIESYGVFDCVMDEGMSQPP+PS+LEKSS
Sbjct: 121 VSSFEASSIIPFLQGSKTVTGNESVSGSTGDEIESYGVFDCVMDEGMSQPPDPSKLEKSS 180

Query: 181 WISRFLNNCSEDAKAIATALTVSVLFRSSLAEPRSIPSSSMYPTLDVGDRILAEKVSYFF 240
           WISRFLNNCSEDAKAIATALTVSVLFRSSLAEPRSIPSSSMYPTLDVGDRILAEKVSYFF
Sbjct: 181 WISRFLNNCSEDAKAIATALTVSVLFRSSLAEPRSIPSSSMYPTLDVGDRILAEKVSYFF 240

Query: 241 RKPSVSDIVIFKAPPILQKIGYKSNDVFIKRIVAKAGDCVEVRDGKLLVNGVAQNEKFIL 300
           R+PSVSDIVIFKAPPILQKIGYKSNDVFIKRIVAKAGDCVEVRDGKLLVNGVAQNEKFIL
Sbjct: 241 RRPSVSDIVIFKAPPILQKIGYKSNDVFIKRIVAKAGDCVEVRDGKLLVNGVAQNEKFIL 300

Query: 301 EPLSYNMDPVLVPEGYVFVLGDNRNNSFDSHNWGPLPVENIVGRSVFRYWPPSKVSDIMS 360
           EPLSYNMDPVLVPEGYVFVLGDNRNNSFDSHNWGPLPVENIVGRSVFRYWPPSKVSD   
Sbjct: 301 EPLSYNMDPVLVPEGYVFVLGDNRNNSFDSHNWGPLPVENIVGRSVFRYWPPSKVSD--K 360

Query: 361 DQNAKKDVVVS 372
           DQNA+K+VVVS
Sbjct: 361 DQNAEKEVVVS 369

BLAST of Cmc07g0184481 vs. NCBI nr
Match: XP_038900232.1 (thylakoidal processing peptidase 1, chloroplastic-like [Benincasa hispida])

HSP 1 Score: 682.6 bits (1760), Expect = 2.0e-192
Identity = 342/371 (92.18%), Postives = 353/371 (95.15%), Query Frame = 0

Query: 1   MAIRVTVSFSGYVAQNLASSAGIRVGNCRAVHECWIRSRLFGSNQKPEFDPAGSVRNYHS 60
           MAIRVTVSFSGYVAQNLA+SAGIRVGNCRAVHECWIRSR FGSNQKPEFD  GSVR+YHS
Sbjct: 1   MAIRVTVSFSGYVAQNLATSAGIRVGNCRAVHECWIRSRFFGSNQKPEFDHPGSVRHYHS 60

Query: 61  DVLPSNSRCWVKNSASALGTIAGEIVDESCRNPIVLGLISLMKSAVGTSVSSPMAMGVFG 120
           DVLPSNSRCWVKNSAS+ GTIAGEIV ES RNPIVLGLIS+MKS V TSVSSPMAMGVFG
Sbjct: 61  DVLPSNSRCWVKNSASSFGTIAGEIVGESFRNPIVLGLISIMKSTVVTSVSSPMAMGVFG 120

Query: 121 VSSFEASSIIPFLQGSKTVTGNESISGSAGDEIESYGVFDCVMDEGMSQPPNPSQLEKSS 180
           VSSF A+S+IPFLQGSK+VTGN+S+  SAG EIESYGVFDCVMDEGMS PPNP QLEKSS
Sbjct: 121 VSSFNAASVIPFLQGSKSVTGNDSVPSSAGGEIESYGVFDCVMDEGMSLPPNPPQLEKSS 180

Query: 181 WISRFLNNCSEDAKAIATALTVSVLFRSSLAEPRSIPSSSMYPTLDVGDRILAEKVSYFF 240
           WISRFLNNCS+DAKAI TALTVSVLFRS LAEPRSIPSSSMYPTLDVGDRILAEKVSYFF
Sbjct: 181 WISRFLNNCSQDAKAIVTALTVSVLFRSFLAEPRSIPSSSMYPTLDVGDRILAEKVSYFF 240

Query: 241 RKPSVSDIVIFKAPPILQKIGYKSNDVFIKRIVAKAGDCVEVRDGKLLVNGVAQNEKFIL 300
           RKPSVSDIVIFKAPPILQ+IGYKSNDVFIKRIVAKAGDCVEVRDGKLLVNGVAQNEKFIL
Sbjct: 241 RKPSVSDIVIFKAPPILQEIGYKSNDVFIKRIVAKAGDCVEVRDGKLLVNGVAQNEKFIL 300

Query: 301 EPLSYNMDPVLVPEGYVFVLGDNRNNSFDSHNWGPLPVENIVGRSVFRYWPPSKVSDIMS 360
           EPLSYNMDPVLVPEGYVFVLGDNRNNSFDSHNWGPLPVENIVGRSVFRYWPPSKVSDIMS
Sbjct: 301 EPLSYNMDPVLVPEGYVFVLGDNRNNSFDSHNWGPLPVENIVGRSVFRYWPPSKVSDIMS 360

Query: 361 DQNAKKDVVVS 372
           DQNA KD VVS
Sbjct: 361 DQNADKDAVVS 371

BLAST of Cmc07g0184481 vs. NCBI nr
Match: XP_023536434.1 (thylakoidal processing peptidase 1, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 657.9 bits (1696), Expect = 5.1e-185
Identity = 328/371 (88.41%), Postives = 347/371 (93.53%), Query Frame = 0

Query: 1   MAIRVTVSFSGYVAQNLASSAGIRVGNCRAVHECWIRSRLFGSNQKPEFDPAGSVRNYHS 60
           MAIRVTVSFS YVAQNLA+SA IRVGNCRAVHECW+RSR+FGSNQKPEFDP+GSVRNY S
Sbjct: 1   MAIRVTVSFSSYVAQNLATSASIRVGNCRAVHECWLRSRIFGSNQKPEFDPSGSVRNYRS 60

Query: 61  DVLPSNSRCWVKNSASALGTIAGEIVDESCRNPIVLGLISLMKSAVGTSVSSPMAMGVFG 120
           DV PSNS+CWVKNSASA GTIAGEIV ESC++PIVLGLIS+MKS VGTS SSPMAMGVFG
Sbjct: 61  DVPPSNSKCWVKNSASAFGTIAGEIVGESCKSPIVLGLISIMKSTVGTSASSPMAMGVFG 120

Query: 121 VSSFEASSIIPFLQGSKTVTGNESISGSAGDEIESYGVFDCVMDEGMSQPPNPSQLEKSS 180
           VSSF A+SIIPFLQGSK +  NESI   A  E+ESYGVFD VMDEG+SQPPNP +LEKSS
Sbjct: 121 VSSFRAASIIPFLQGSKWLPCNESIPALASGELESYGVFDSVMDEGISQPPNPPRLEKSS 180

Query: 181 WISRFLNNCSEDAKAIATALTVSVLFRSSLAEPRSIPSSSMYPTLDVGDRILAEKVSYFF 240
           WISRFLNNCSEDAKAIATA TVSVLFRS LAEPRSIPSSSMYPTLDVGDRILAEKVSYFF
Sbjct: 181 WISRFLNNCSEDAKAIATAFTVSVLFRSFLAEPRSIPSSSMYPTLDVGDRILAEKVSYFF 240

Query: 241 RKPSVSDIVIFKAPPILQKIGYKSNDVFIKRIVAKAGDCVEVRDGKLLVNGVAQNEKFIL 300
           RKPSVSD+VIFKAPPILQ+IGYKS+DVFIKRIVAKAGDCVEVRDGKLLVNGVAQNEKFIL
Sbjct: 241 RKPSVSDVVIFKAPPILQEIGYKSSDVFIKRIVAKAGDCVEVRDGKLLVNGVAQNEKFIL 300

Query: 301 EPLSYNMDPVLVPEGYVFVLGDNRNNSFDSHNWGPLPVENIVGRSVFRYWPPSKVSDIMS 360
           EPLSYNMDPVLVPEGYVFV+GDNRNNSFDSHNWGPLPVENIVGRSVFRYWPPSKVSD  S
Sbjct: 301 EPLSYNMDPVLVPEGYVFVMGDNRNNSFDSHNWGPLPVENIVGRSVFRYWPPSKVSDTAS 360

Query: 361 DQNAKKDVVVS 372
           +QN+ KDVVVS
Sbjct: 361 EQNSGKDVVVS 371

BLAST of Cmc07g0184481 vs. NCBI nr
Match: KAG6591353.1 (putative thylakoidal processing peptidase 2, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 656.4 bits (1692), Expect = 1.5e-184
Identity = 327/371 (88.14%), Postives = 345/371 (92.99%), Query Frame = 0

Query: 1   MAIRVTVSFSGYVAQNLASSAGIRVGNCRAVHECWIRSRLFGSNQKPEFDPAGSVRNYHS 60
           MAIRVTVSFS YVAQNLA+SA IRVGNCRAVHECW+RSR+FGSNQKPEFDP+GSVRNY S
Sbjct: 1   MAIRVTVSFSSYVAQNLATSASIRVGNCRAVHECWLRSRIFGSNQKPEFDPSGSVRNYRS 60

Query: 61  DVLPSNSRCWVKNSASALGTIAGEIVDESCRNPIVLGLISLMKSAVGTSVSSPMAMGVFG 120
           DV PSNS+CWVKNSASA GTIAGEIV ESC++PIVLGLIS+MKS VGTS SSPM MGVFG
Sbjct: 61  DVPPSNSKCWVKNSASAFGTIAGEIVGESCKSPIVLGLISIMKSTVGTSASSPMTMGVFG 120

Query: 121 VSSFEASSIIPFLQGSKTVTGNESISGSAGDEIESYGVFDCVMDEGMSQPPNPSQLEKSS 180
           VSSF A+SIIPFLQGSK +  NESI   A  E+ESYGVFD  MDEGMSQPPNP +LEKSS
Sbjct: 121 VSSFRAASIIPFLQGSKWLPCNESIPALASGELESYGVFDSAMDEGMSQPPNPPRLEKSS 180

Query: 181 WISRFLNNCSEDAKAIATALTVSVLFRSSLAEPRSIPSSSMYPTLDVGDRILAEKVSYFF 240
           WISRFLNNCSEDAKAIATA TVSVLFRS LAEPRSIPSSSMYPTLDVGDRILAEKVSYFF
Sbjct: 181 WISRFLNNCSEDAKAIATAFTVSVLFRSFLAEPRSIPSSSMYPTLDVGDRILAEKVSYFF 240

Query: 241 RKPSVSDIVIFKAPPILQKIGYKSNDVFIKRIVAKAGDCVEVRDGKLLVNGVAQNEKFIL 300
           RKPSVSD+VIFKAPPILQ+IGYKS+DVFIKRIVAKAGDCVEVRDGKLLVNGVAQNEKFIL
Sbjct: 241 RKPSVSDVVIFKAPPILQEIGYKSSDVFIKRIVAKAGDCVEVRDGKLLVNGVAQNEKFIL 300

Query: 301 EPLSYNMDPVLVPEGYVFVLGDNRNNSFDSHNWGPLPVENIVGRSVFRYWPPSKVSDIMS 360
           EPLSYNMDPVLVPEGYVFV+GDNRNNSFDSHNWGPLPVENIVGRSVFRYWPPSKVSD  S
Sbjct: 301 EPLSYNMDPVLVPEGYVFVMGDNRNNSFDSHNWGPLPVENIVGRSVFRYWPPSKVSDTAS 360

Query: 361 DQNAKKDVVVS 372
           +QN+ KDVVVS
Sbjct: 361 EQNSGKDVVVS 371

BLAST of Cmc07g0184481 vs. ExPASy Swiss-Prot
Match: Q9M9Z2 (Probable thylakoidal processing peptidase 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=TPP2 PE=2 SV=1)

HSP 1 Score: 363.6 bits (932), Expect = 2.6e-99
Identity = 203/376 (53.99%), Postives = 255/376 (67.82%), Query Frame = 0

Query: 1   MAIRVTVSFSGYVAQNLASSAGIRV--GNCRAVHECWIRSRLFGSNQKPEFDPAGSVRNY 60
           MAIRVT ++S YVA+++ASSAG RV  G+ R+  E W+R R  G NQ P+       ++ 
Sbjct: 1   MAIRVTFTYSSYVARSIASSAGTRVGTGDVRSCFETWVRPRFCGHNQIPDIVD----KSP 60

Query: 61  HSDVLPSNSRCWVKNSASALGTIAGEIVDESCRNPIVLGLISLMKSAVGTSVSSPMAMGV 120
            S+    +S    + ++S   TIA EI++E C++P+VLG+ISLM     T       M  
Sbjct: 61  GSNTWGPSSGPRARPASSMYSTIAREILEEGCKSPLVLGMISLMNL---TGAPQFSGMTG 120

Query: 121 FGVSSFEASSIIPFLQGSKTVTGNESISGSAGDEIESYGVFDCVMDEGMSQPPNPSQLEK 180
            G+S F+ SS+IPFL+GSK +    SI  +   +I         +D G        +LE 
Sbjct: 121 LGISPFKTSSVIPFLRGSKWMPC--SIPATLSTDIAE-------VDRGGKVCDPKVKLEL 180

Query: 181 S--------SWISRFLNNCSEDAKAIATALTVSVLFRSSLAEPRSIPSSSMYPTLDVGDR 240
           S         W+++ LN CSEDAKA  TA+TVS+LFRS+LAEP+SIPS+SM PTLDVGDR
Sbjct: 181 SDKVSNGGNGWVNKLLNICSEDAKAAFTAVTVSLLFRSALAEPKSIPSTSMLPTLDVGDR 240

Query: 241 ILAEKVSYFFRKPSVSDIVIFKAPPILQKIGYKSNDVFIKRIVAKAGDCVEVRDGKLLVN 300
           ++AEKVSYFFRKP VSDIVIFKAPPIL + GY   DVFIKRIVA  GD VEV DGKLLVN
Sbjct: 241 VIAEKVSYFFRKPEVSDIVIFKAPPILVEHGYSCADVFIKRIVASEGDWVEVCDGKLLVN 300

Query: 301 GVAQNEKFILEPLSYNMDPVLVPEGYVFVLGDNRNNSFDSHNWGPLPVENIVGRSVFRYW 360
              Q E F+LEP+ Y M+P+ VPEGYVFVLGDNRN SFDSHNWGPLP++NI+GRSVFRYW
Sbjct: 301 DTVQAEDFVLEPIDYEMEPMFVPEGYVFVLGDNRNKSFDSHNWGPLPIKNIIGRSVFRYW 360

Query: 361 PPSKVSDIMSDQNAKK 367
           PPSKVSDI+  +   +
Sbjct: 361 PPSKVSDIIHHEQVSQ 360

BLAST of Cmc07g0184481 vs. ExPASy Swiss-Prot
Match: O04348 (Thylakoidal processing peptidase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=TPP1 PE=2 SV=2)

HSP 1 Score: 361.3 bits (926), Expect = 1.3e-98
Identity = 207/366 (56.56%), Postives = 252/366 (68.85%), Query Frame = 0

Query: 1   MAIRVTVSFSGYVAQNLASSAGIRVGNCRAVHECWIRSRLFGSNQKPEFDPAGSVRNYHS 60
           MAIR+T ++S +VA+NL    G RVG      E  +R R F  + K +FD +        
Sbjct: 1   MAIRITFTYSTHVARNL---VGTRVGPGGYCFESLVRPRFF--SHKRDFDRS-------- 60

Query: 61  DVLPSNSRCWVKNSASALGTIAGEIVDESCRNPIVLGLISLMKSAVGTSVSSPMAMGVFG 120
              P N        AS  G+IA E++ E  ++P+V+GLIS++KS  G   S+   M V G
Sbjct: 61  ---PRN------RPASMYGSIARELIGEGSQSPLVMGLISILKSTTGHESST---MNVLG 120

Query: 121 VSSFEASSIIPFLQGSKTVTGNESISGSAGDEIESYGVFDCVMDEGMSQPPNPSQLEKSS 180
           VSSF+ASSIIPFLQGSK +     I     D+++  G    V D+        S+   S 
Sbjct: 121 VSSFKASSIIPFLQGSKWIKNPPVI-----DDVDKGGT---VCDD---DDDKESRNGGSG 180

Query: 181 WISRFLNNCSEDAKAIATALTVSVLFRSSLAEPRSIPSSSMYPTLDVGDRILAEKVSYFF 240
           W+++ L+ CSEDAKA  TA+TVS+LFRS+LAEP+SIPS+SMYPTLD GDR++AEKVSYFF
Sbjct: 181 WVNKLLSVCSEDAKAAFTAVTVSILFRSALAEPKSIPSTSMYPTLDKGDRVMAEKVSYFF 240

Query: 241 RKPSVSDIVIFKAPPIL---QKIGYKSNDVFIKRIVAKAGDCVEVRDGKLLVNGVAQNEK 300
           RKP VSDIVIFKAPPIL    + GY SNDVFIKRIVA  GD VEVRDGKL VN + Q E 
Sbjct: 241 RKPEVSDIVIFKAPPILLEYPEYGYSSNDVFIKRIVASEGDWVEVRDGKLFVNDIVQEED 300

Query: 301 FILEPLSYNMDPVLVPEGYVFVLGDNRNNSFDSHNWGPLPVENIVGRSVFRYWPPSKVSD 360
           F+LEP+SY M+P+ VP+GYVFVLGDNRN SFDSHNWGPLP+ENIVGRSVFRYWPPSKVSD
Sbjct: 301 FVLEPMSYEMEPMFVPKGYVFVLGDNRNKSFDSHNWGPLPIENIVGRSVFRYWPPSKVSD 330

Query: 361 -IMSDQ 363
            I  DQ
Sbjct: 361 TIYHDQ 330

BLAST of Cmc07g0184481 vs. ExPASy Swiss-Prot
Match: Q8H0W1 (Chloroplast processing peptidase OS=Arabidopsis thaliana OX=3702 GN=PLSP1 PE=2 SV=2)

HSP 1 Score: 255.0 bits (650), Expect = 1.3e-66
Identity = 119/180 (66.11%), Postives = 147/180 (81.67%), Query Frame = 0

Query: 177 EKSSWISRFLNNCSEDAKAIATALTVSVLFRSSLAEPRSIPSSSMYPTLDVGDRILAEKV 236
           EK+     +L+  S+DA+ +  A+ VS+ FR  +AEPR IPS SMYPT DVGDR++AEKV
Sbjct: 99  EKNRLFPEWLDFTSDDAQTVFVAIAVSLAFRYFIAEPRYIPSLSMYPTFDVGDRLVAEKV 158

Query: 237 SYFFRKPSVSDIVIFKAPPILQKIGYKSNDVFIKRIVAKAGDCVEVRDGKLLVNGVAQNE 296
           SY+FRKP  +DIVIFK+PP+LQ++GY   DVFIKRIVAK GD VEV +GKL+VNGVA+NE
Sbjct: 159 SYYFRKPCANDIVIFKSPPVLQEVGYTDADVFIKRIVAKEGDLVEVHNGKLMVNGVARNE 218

Query: 297 KFILEPLSYNMDPVLVPEGYVFVLGDNRNNSFDSHNWGPLPVENIVGRSVFRYWPPSKVS 356
           KFILEP  Y M P+ VPE  VFV+GDNRNNS+DSH WGPLP++NI+GRSVFRYWPP++VS
Sbjct: 219 KFILEPPGYEMTPIRVPENSVFVMGDNRNNSYDSHVWGPLPLKNIIGRSVFRYWPPNRVS 278

BLAST of Cmc07g0184481 vs. ExPASy Swiss-Prot
Match: P72660 (Probable signal peptidase I-1 OS=Synechocystis sp. (strain PCC 6803 / Kazusa) OX=1111708 GN=lepB1 PE=3 SV=1)

HSP 1 Score: 171.0 bits (432), Expect = 2.5e-41
Identity = 82/164 (50.00%), Postives = 111/164 (67.68%), Query Frame = 0

Query: 191 EDAKAIATALTVSVLFRSSLAEPRSIPSSSMYPTLDVGDRILAEKVSYFFRKPSVSDIVI 250
           E+   +  AL +++L R  +AEPR IPS SM PTL+ GDR++ EKVSY F  P V DI++
Sbjct: 15  ENIPLLMVALVLALLLRFFVAEPRYIPSDSMLPTLEQGDRLVVEKVSYHFHPPQVGDIIV 74

Query: 251 FKAPPILQKIGYKSNDVFIKRIVAKAGDCVEVRDGKLLVNGVAQNEKFILEPLSYNMDPV 310
           F  P +LQ  GY     FIKR++A  G  VEV +G +  +G    E++ILEP  YN+  V
Sbjct: 75  FHPPELLQVQGYDLGQAFIKRVIALPGQTVEVNNGIVYRDGQPLQEEYILEPPQYNLPAV 134

Query: 311 LVPEGYVFVLGDNRNNSFDSHNWGPLPVENIVGRSVFRYWPPSK 355
            VP+G VFV+GDNRNNS DSH WG LP +NI+G ++FR++P S+
Sbjct: 135 RVPDGQVFVMGDNRNNSNDSHVWGFLPQQNIIGHALFRFFPASR 178

BLAST of Cmc07g0184481 vs. ExPASy Swiss-Prot
Match: Q51876 (Signal peptidase I OS=Phormidium laminosum OX=32059 GN=lepB PE=3 SV=1)

HSP 1 Score: 151.8 bits (382), Expect = 1.6e-35
Identity = 70/168 (41.67%), Postives = 105/168 (62.50%), Query Frame = 0

Query: 191 EDAKAIATALTVSVLFRSSLAEPRSIPSSSMYPTLDVGDRILAEKVSYFFRKPSVSDIVI 250
           E  K I  ++ +++  R+ +AE R IPS SM PTL+V DR++ EK+SY F  P   DI++
Sbjct: 30  EGIKTIGLSVVLALGIRTFVAEARYIPSESMLPTLEVNDRLIVEKISYHFNPPRRGDIIV 89

Query: 251 FKAPPILQKIGYKSNDVFIKRIVAKAGDCVEVRDGKLLVNGVAQNEKFILEPLSYNMDPV 310
           F     L++     N+ FIKR++   G+ V+V  G++L+NG    E +I  P  Y   P 
Sbjct: 90  FHPTEALKQQNPSLNEAFIKRVIGLPGETVQVTGGRVLINGQPLEENYIQSPPDYQWGPE 149

Query: 311 LVPEGYVFVLGDNRNNSFDSHNWGPLPVENIVGRSVFRYWPPSKVSDI 359
            VP     VLGDNRNNS+DSH WG +P +NI+GR+V R+WP +++ ++
Sbjct: 150 KVPADSFLVLGDNRNNSYDSHFWGYVPRQNIIGRAVVRFWPVNRLGEL 197

BLAST of Cmc07g0184481 vs. ExPASy TrEMBL
Match: A0A5A7V7L7 (Thylakoidal processing peptidase 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold134G003740 PE=4 SV=1)

HSP 1 Score: 736.5 bits (1900), Expect = 5.5e-209
Identity = 371/371 (100.00%), Postives = 371/371 (100.00%), Query Frame = 0

Query: 1   MAIRVTVSFSGYVAQNLASSAGIRVGNCRAVHECWIRSRLFGSNQKPEFDPAGSVRNYHS 60
           MAIRVTVSFSGYVAQNLASSAGIRVGNCRAVHECWIRSRLFGSNQKPEFDPAGSVRNYHS
Sbjct: 1   MAIRVTVSFSGYVAQNLASSAGIRVGNCRAVHECWIRSRLFGSNQKPEFDPAGSVRNYHS 60

Query: 61  DVLPSNSRCWVKNSASALGTIAGEIVDESCRNPIVLGLISLMKSAVGTSVSSPMAMGVFG 120
           DVLPSNSRCWVKNSASALGTIAGEIVDESCRNPIVLGLISLMKSAVGTSVSSPMAMGVFG
Sbjct: 61  DVLPSNSRCWVKNSASALGTIAGEIVDESCRNPIVLGLISLMKSAVGTSVSSPMAMGVFG 120

Query: 121 VSSFEASSIIPFLQGSKTVTGNESISGSAGDEIESYGVFDCVMDEGMSQPPNPSQLEKSS 180
           VSSFEASSIIPFLQGSKTVTGNESISGSAGDEIESYGVFDCVMDEGMSQPPNPSQLEKSS
Sbjct: 121 VSSFEASSIIPFLQGSKTVTGNESISGSAGDEIESYGVFDCVMDEGMSQPPNPSQLEKSS 180

Query: 181 WISRFLNNCSEDAKAIATALTVSVLFRSSLAEPRSIPSSSMYPTLDVGDRILAEKVSYFF 240
           WISRFLNNCSEDAKAIATALTVSVLFRSSLAEPRSIPSSSMYPTLDVGDRILAEKVSYFF
Sbjct: 181 WISRFLNNCSEDAKAIATALTVSVLFRSSLAEPRSIPSSSMYPTLDVGDRILAEKVSYFF 240

Query: 241 RKPSVSDIVIFKAPPILQKIGYKSNDVFIKRIVAKAGDCVEVRDGKLLVNGVAQNEKFIL 300
           RKPSVSDIVIFKAPPILQKIGYKSNDVFIKRIVAKAGDCVEVRDGKLLVNGVAQNEKFIL
Sbjct: 241 RKPSVSDIVIFKAPPILQKIGYKSNDVFIKRIVAKAGDCVEVRDGKLLVNGVAQNEKFIL 300

Query: 301 EPLSYNMDPVLVPEGYVFVLGDNRNNSFDSHNWGPLPVENIVGRSVFRYWPPSKVSDIMS 360
           EPLSYNMDPVLVPEGYVFVLGDNRNNSFDSHNWGPLPVENIVGRSVFRYWPPSKVSDIMS
Sbjct: 301 EPLSYNMDPVLVPEGYVFVLGDNRNNSFDSHNWGPLPVENIVGRSVFRYWPPSKVSDIMS 360

Query: 361 DQNAKKDVVVS 372
           DQNAKKDVVVS
Sbjct: 361 DQNAKKDVVVS 371

BLAST of Cmc07g0184481 vs. ExPASy TrEMBL
Match: A0A1S3BTW2 (thylakoidal processing peptidase 1, chloroplastic-like OS=Cucumis melo OX=3656 GN=LOC103493506 PE=4 SV=1)

HSP 1 Score: 736.5 bits (1900), Expect = 5.5e-209
Identity = 371/371 (100.00%), Postives = 371/371 (100.00%), Query Frame = 0

Query: 1   MAIRVTVSFSGYVAQNLASSAGIRVGNCRAVHECWIRSRLFGSNQKPEFDPAGSVRNYHS 60
           MAIRVTVSFSGYVAQNLASSAGIRVGNCRAVHECWIRSRLFGSNQKPEFDPAGSVRNYHS
Sbjct: 1   MAIRVTVSFSGYVAQNLASSAGIRVGNCRAVHECWIRSRLFGSNQKPEFDPAGSVRNYHS 60

Query: 61  DVLPSNSRCWVKNSASALGTIAGEIVDESCRNPIVLGLISLMKSAVGTSVSSPMAMGVFG 120
           DVLPSNSRCWVKNSASALGTIAGEIVDESCRNPIVLGLISLMKSAVGTSVSSPMAMGVFG
Sbjct: 61  DVLPSNSRCWVKNSASALGTIAGEIVDESCRNPIVLGLISLMKSAVGTSVSSPMAMGVFG 120

Query: 121 VSSFEASSIIPFLQGSKTVTGNESISGSAGDEIESYGVFDCVMDEGMSQPPNPSQLEKSS 180
           VSSFEASSIIPFLQGSKTVTGNESISGSAGDEIESYGVFDCVMDEGMSQPPNPSQLEKSS
Sbjct: 121 VSSFEASSIIPFLQGSKTVTGNESISGSAGDEIESYGVFDCVMDEGMSQPPNPSQLEKSS 180

Query: 181 WISRFLNNCSEDAKAIATALTVSVLFRSSLAEPRSIPSSSMYPTLDVGDRILAEKVSYFF 240
           WISRFLNNCSEDAKAIATALTVSVLFRSSLAEPRSIPSSSMYPTLDVGDRILAEKVSYFF
Sbjct: 181 WISRFLNNCSEDAKAIATALTVSVLFRSSLAEPRSIPSSSMYPTLDVGDRILAEKVSYFF 240

Query: 241 RKPSVSDIVIFKAPPILQKIGYKSNDVFIKRIVAKAGDCVEVRDGKLLVNGVAQNEKFIL 300
           RKPSVSDIVIFKAPPILQKIGYKSNDVFIKRIVAKAGDCVEVRDGKLLVNGVAQNEKFIL
Sbjct: 241 RKPSVSDIVIFKAPPILQKIGYKSNDVFIKRIVAKAGDCVEVRDGKLLVNGVAQNEKFIL 300

Query: 301 EPLSYNMDPVLVPEGYVFVLGDNRNNSFDSHNWGPLPVENIVGRSVFRYWPPSKVSDIMS 360
           EPLSYNMDPVLVPEGYVFVLGDNRNNSFDSHNWGPLPVENIVGRSVFRYWPPSKVSDIMS
Sbjct: 301 EPLSYNMDPVLVPEGYVFVLGDNRNNSFDSHNWGPLPVENIVGRSVFRYWPPSKVSDIMS 360

Query: 361 DQNAKKDVVVS 372
           DQNAKKDVVVS
Sbjct: 361 DQNAKKDVVVS 371

BLAST of Cmc07g0184481 vs. ExPASy TrEMBL
Match: A0A0A0L290 (Peptidase_S26 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G638430 PE=4 SV=1)

HSP 1 Score: 712.6 bits (1838), Expect = 8.5e-202
Identity = 359/371 (96.77%), Postives = 366/371 (98.65%), Query Frame = 0

Query: 1   MAIRVTVSFSGYVAQNLASSAGIRVGNCRAVHECWIRSRLFGSNQKPEFDPAGSVRNYHS 60
           MAIRVTVSFSGYVAQNLASSAGIRVGNCRAVHECWIRSRLFGSNQKPEFDP+GSVRNYHS
Sbjct: 1   MAIRVTVSFSGYVAQNLASSAGIRVGNCRAVHECWIRSRLFGSNQKPEFDPSGSVRNYHS 60

Query: 61  DVLPSNSRCWVKNSASALGTIAGEIVDESCRNPIVLGLISLMKSAVGTSVSSPMAMGVFG 120
            VLPSNSRCWVKNSASALGTIAGEIVDESCRNPIVLGLISLMKSAVGTSVSSPMAMGVFG
Sbjct: 61  AVLPSNSRCWVKNSASALGTIAGEIVDESCRNPIVLGLISLMKSAVGTSVSSPMAMGVFG 120

Query: 121 VSSFEASSIIPFLQGSKTVTGNESISGSAGDEIESYGVFDCVMDEGMSQPPNPSQLEKSS 180
           VSSFEASSIIPFLQGSKTVTGNES+SGS GDEIESYGVFDCVMDEGMSQPP+PS+LEKSS
Sbjct: 121 VSSFEASSIIPFLQGSKTVTGNESVSGSTGDEIESYGVFDCVMDEGMSQPPDPSKLEKSS 180

Query: 181 WISRFLNNCSEDAKAIATALTVSVLFRSSLAEPRSIPSSSMYPTLDVGDRILAEKVSYFF 240
           WISRFLNNCSEDAKAIATALTVSVLFRSSLAEPRSIPSSSMYPTLDVGDRILAEKVSYFF
Sbjct: 181 WISRFLNNCSEDAKAIATALTVSVLFRSSLAEPRSIPSSSMYPTLDVGDRILAEKVSYFF 240

Query: 241 RKPSVSDIVIFKAPPILQKIGYKSNDVFIKRIVAKAGDCVEVRDGKLLVNGVAQNEKFIL 300
           R+PSVSDIVIFKAPPILQKIGYKSNDVFIKRIVAKAGDCVEVRDGKLLVNGVAQNEKFIL
Sbjct: 241 RRPSVSDIVIFKAPPILQKIGYKSNDVFIKRIVAKAGDCVEVRDGKLLVNGVAQNEKFIL 300

Query: 301 EPLSYNMDPVLVPEGYVFVLGDNRNNSFDSHNWGPLPVENIVGRSVFRYWPPSKVSDIMS 360
           EPLSYNMDPVLVPEGYVFVLGDNRNNSFDSHNWGPLPVENIVGRSVFRYWPPSKVSD   
Sbjct: 301 EPLSYNMDPVLVPEGYVFVLGDNRNNSFDSHNWGPLPVENIVGRSVFRYWPPSKVSD--K 360

Query: 361 DQNAKKDVVVS 372
           DQNA+K+VVVS
Sbjct: 361 DQNAEKEVVVS 369

BLAST of Cmc07g0184481 vs. ExPASy TrEMBL
Match: A0A6J1FC76 (thylakoidal processing peptidase 1, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111442773 PE=4 SV=1)

HSP 1 Score: 655.6 bits (1690), Expect = 1.2e-184
Identity = 327/371 (88.14%), Postives = 345/371 (92.99%), Query Frame = 0

Query: 1   MAIRVTVSFSGYVAQNLASSAGIRVGNCRAVHECWIRSRLFGSNQKPEFDPAGSVRNYHS 60
           MAIRVTVSFS YVAQNLA+SA IRVGNCRAVHECW+RSR+FGSNQKPEFDP+GSVRNY S
Sbjct: 1   MAIRVTVSFSSYVAQNLATSASIRVGNCRAVHECWLRSRIFGSNQKPEFDPSGSVRNYRS 60

Query: 61  DVLPSNSRCWVKNSASALGTIAGEIVDESCRNPIVLGLISLMKSAVGTSVSSPMAMGVFG 120
           DV PSNS+CWVKNSASA GTIAGEIV ESC++PIVLGLIS+MKS VGTS SSPMAMGVFG
Sbjct: 61  DVPPSNSKCWVKNSASAFGTIAGEIVGESCKSPIVLGLISIMKSTVGTSASSPMAMGVFG 120

Query: 121 VSSFEASSIIPFLQGSKTVTGNESISGSAGDEIESYGVFDCVMDEGMSQPPNPSQLEKSS 180
           VSSF A+SIIPFLQGSK +  NESI   A  E+ESYGVFD  MDE MSQPPNP +LEKSS
Sbjct: 121 VSSFRAASIIPFLQGSKWLPCNESIPALASGELESYGVFDSAMDESMSQPPNPPRLEKSS 180

Query: 181 WISRFLNNCSEDAKAIATALTVSVLFRSSLAEPRSIPSSSMYPTLDVGDRILAEKVSYFF 240
           WISRFLNNCSEDAKAIATA TVSVLFRS LAEPRSIPSSSMYPTLDVGDRILAEKVSYFF
Sbjct: 181 WISRFLNNCSEDAKAIATAFTVSVLFRSFLAEPRSIPSSSMYPTLDVGDRILAEKVSYFF 240

Query: 241 RKPSVSDIVIFKAPPILQKIGYKSNDVFIKRIVAKAGDCVEVRDGKLLVNGVAQNEKFIL 300
           RKPSVSD+VIFKAPPILQ+IGYKS+DVFIKRIVAKAGDCVEVRDGKLLVNGVAQNEKFIL
Sbjct: 241 RKPSVSDVVIFKAPPILQEIGYKSSDVFIKRIVAKAGDCVEVRDGKLLVNGVAQNEKFIL 300

Query: 301 EPLSYNMDPVLVPEGYVFVLGDNRNNSFDSHNWGPLPVENIVGRSVFRYWPPSKVSDIMS 360
           EPLSYNMDPVLVPEGYVFV+GDNRNNSFDSHNWGPLPVENIVGRSVFRYWPPSKVSD  S
Sbjct: 301 EPLSYNMDPVLVPEGYVFVMGDNRNNSFDSHNWGPLPVENIVGRSVFRYWPPSKVSDTAS 360

Query: 361 DQNAKKDVVVS 372
           +QN+ KDVVVS
Sbjct: 361 EQNSGKDVVVS 371

BLAST of Cmc07g0184481 vs. ExPASy TrEMBL
Match: A0A6J1IF81 (thylakoidal processing peptidase 1, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111476692 PE=4 SV=1)

HSP 1 Score: 652.5 bits (1682), Expect = 1.0e-183
Identity = 325/371 (87.60%), Postives = 344/371 (92.72%), Query Frame = 0

Query: 1   MAIRVTVSFSGYVAQNLASSAGIRVGNCRAVHECWIRSRLFGSNQKPEFDPAGSVRNYHS 60
           MAIRVTVSFS YVAQNLA+SA IRVG+CRAVHECW+RSR+FGSNQKPEFDP+GSVRNY S
Sbjct: 1   MAIRVTVSFSSYVAQNLATSASIRVGSCRAVHECWLRSRIFGSNQKPEFDPSGSVRNYRS 60

Query: 61  DVLPSNSRCWVKNSASALGTIAGEIVDESCRNPIVLGLISLMKSAVGTSVSSPMAMGVFG 120
           DV PSNS+CWVKNSASA GTIAGEIV ESC++PIVLGLIS+MKS  GTS SSPMAMGVFG
Sbjct: 61  DVPPSNSKCWVKNSASAFGTIAGEIVGESCKSPIVLGLISIMKSTAGTSASSPMAMGVFG 120

Query: 121 VSSFEASSIIPFLQGSKTVTGNESISGSAGDEIESYGVFDCVMDEGMSQPPNPSQLEKSS 180
           VSSF A+SIIPFLQGSK +  NESI   A  E+ESYGVFD  MDEGMSQPPNP +LEKSS
Sbjct: 121 VSSFRAASIIPFLQGSKWLPCNESIPALASGELESYGVFDSAMDEGMSQPPNPPRLEKSS 180

Query: 181 WISRFLNNCSEDAKAIATALTVSVLFRSSLAEPRSIPSSSMYPTLDVGDRILAEKVSYFF 240
           WISRFLNNCSEDAKAIATA TVSVLFRS LAEPRSIPSSSMYPTLDVGDRILAEKVSYFF
Sbjct: 181 WISRFLNNCSEDAKAIATAFTVSVLFRSFLAEPRSIPSSSMYPTLDVGDRILAEKVSYFF 240

Query: 241 RKPSVSDIVIFKAPPILQKIGYKSNDVFIKRIVAKAGDCVEVRDGKLLVNGVAQNEKFIL 300
           RKPSVSD+VIFKAPPILQ+IGYKS+DVFIKRIVAKAGDCVEVRDGKLLVNGVAQNEKFIL
Sbjct: 241 RKPSVSDVVIFKAPPILQEIGYKSSDVFIKRIVAKAGDCVEVRDGKLLVNGVAQNEKFIL 300

Query: 301 EPLSYNMDPVLVPEGYVFVLGDNRNNSFDSHNWGPLPVENIVGRSVFRYWPPSKVSDIMS 360
           EPLSYNMDPVLVPEGYVFV+GDNRNNSFDSHNWGPLPVENIVGRSVFRYWPPSKVSD   
Sbjct: 301 EPLSYNMDPVLVPEGYVFVMGDNRNNSFDSHNWGPLPVENIVGRSVFRYWPPSKVSDTAC 360

Query: 361 DQNAKKDVVVS 372
           +QN+ KDVVVS
Sbjct: 361 EQNSGKDVVVS 371

BLAST of Cmc07g0184481 vs. TAIR 10
Match: AT1G06870.1 (Peptidase S24/S26A/S26B/S26C family protein )

HSP 1 Score: 363.6 bits (932), Expect = 1.9e-100
Identity = 203/376 (53.99%), Postives = 255/376 (67.82%), Query Frame = 0

Query: 1   MAIRVTVSFSGYVAQNLASSAGIRV--GNCRAVHECWIRSRLFGSNQKPEFDPAGSVRNY 60
           MAIRVT ++S YVA+++ASSAG RV  G+ R+  E W+R R  G NQ P+       ++ 
Sbjct: 1   MAIRVTFTYSSYVARSIASSAGTRVGTGDVRSCFETWVRPRFCGHNQIPDIVD----KSP 60

Query: 61  HSDVLPSNSRCWVKNSASALGTIAGEIVDESCRNPIVLGLISLMKSAVGTSVSSPMAMGV 120
            S+    +S    + ++S   TIA EI++E C++P+VLG+ISLM     T       M  
Sbjct: 61  GSNTWGPSSGPRARPASSMYSTIAREILEEGCKSPLVLGMISLMNL---TGAPQFSGMTG 120

Query: 121 FGVSSFEASSIIPFLQGSKTVTGNESISGSAGDEIESYGVFDCVMDEGMSQPPNPSQLEK 180
            G+S F+ SS+IPFL+GSK +    SI  +   +I         +D G        +LE 
Sbjct: 121 LGISPFKTSSVIPFLRGSKWMPC--SIPATLSTDIAE-------VDRGGKVCDPKVKLEL 180

Query: 181 S--------SWISRFLNNCSEDAKAIATALTVSVLFRSSLAEPRSIPSSSMYPTLDVGDR 240
           S         W+++ LN CSEDAKA  TA+TVS+LFRS+LAEP+SIPS+SM PTLDVGDR
Sbjct: 181 SDKVSNGGNGWVNKLLNICSEDAKAAFTAVTVSLLFRSALAEPKSIPSTSMLPTLDVGDR 240

Query: 241 ILAEKVSYFFRKPSVSDIVIFKAPPILQKIGYKSNDVFIKRIVAKAGDCVEVRDGKLLVN 300
           ++AEKVSYFFRKP VSDIVIFKAPPIL + GY   DVFIKRIVA  GD VEV DGKLLVN
Sbjct: 241 VIAEKVSYFFRKPEVSDIVIFKAPPILVEHGYSCADVFIKRIVASEGDWVEVCDGKLLVN 300

Query: 301 GVAQNEKFILEPLSYNMDPVLVPEGYVFVLGDNRNNSFDSHNWGPLPVENIVGRSVFRYW 360
              Q E F+LEP+ Y M+P+ VPEGYVFVLGDNRN SFDSHNWGPLP++NI+GRSVFRYW
Sbjct: 301 DTVQAEDFVLEPIDYEMEPMFVPEGYVFVLGDNRNKSFDSHNWGPLPIKNIIGRSVFRYW 360

Query: 361 PPSKVSDIMSDQNAKK 367
           PPSKVSDI+  +   +
Sbjct: 361 PPSKVSDIIHHEQVSQ 360

BLAST of Cmc07g0184481 vs. TAIR 10
Match: AT2G30440.1 (thylakoid processing peptide )

HSP 1 Score: 361.3 bits (926), Expect = 9.3e-100
Identity = 207/366 (56.56%), Postives = 252/366 (68.85%), Query Frame = 0

Query: 1   MAIRVTVSFSGYVAQNLASSAGIRVGNCRAVHECWIRSRLFGSNQKPEFDPAGSVRNYHS 60
           MAIR+T ++S +VA+NL    G RVG      E  +R R F  + K +FD +        
Sbjct: 1   MAIRITFTYSTHVARNL---VGTRVGPGGYCFESLVRPRFF--SHKRDFDRS-------- 60

Query: 61  DVLPSNSRCWVKNSASALGTIAGEIVDESCRNPIVLGLISLMKSAVGTSVSSPMAMGVFG 120
              P N        AS  G+IA E++ E  ++P+V+GLIS++KS  G   S+   M V G
Sbjct: 61  ---PRN------RPASMYGSIARELIGEGSQSPLVMGLISILKSTTGHESST---MNVLG 120

Query: 121 VSSFEASSIIPFLQGSKTVTGNESISGSAGDEIESYGVFDCVMDEGMSQPPNPSQLEKSS 180
           VSSF+ASSIIPFLQGSK +     I     D+++  G    V D+        S+   S 
Sbjct: 121 VSSFKASSIIPFLQGSKWIKNPPVI-----DDVDKGGT---VCDD---DDDKESRNGGSG 180

Query: 181 WISRFLNNCSEDAKAIATALTVSVLFRSSLAEPRSIPSSSMYPTLDVGDRILAEKVSYFF 240
           W+++ L+ CSEDAKA  TA+TVS+LFRS+LAEP+SIPS+SMYPTLD GDR++AEKVSYFF
Sbjct: 181 WVNKLLSVCSEDAKAAFTAVTVSILFRSALAEPKSIPSTSMYPTLDKGDRVMAEKVSYFF 240

Query: 241 RKPSVSDIVIFKAPPIL---QKIGYKSNDVFIKRIVAKAGDCVEVRDGKLLVNGVAQNEK 300
           RKP VSDIVIFKAPPIL    + GY SNDVFIKRIVA  GD VEVRDGKL VN + Q E 
Sbjct: 241 RKPEVSDIVIFKAPPILLEYPEYGYSSNDVFIKRIVASEGDWVEVRDGKLFVNDIVQEED 300

Query: 301 FILEPLSYNMDPVLVPEGYVFVLGDNRNNSFDSHNWGPLPVENIVGRSVFRYWPPSKVSD 360
           F+LEP+SY M+P+ VP+GYVFVLGDNRN SFDSHNWGPLP+ENIVGRSVFRYWPPSKVSD
Sbjct: 301 FVLEPMSYEMEPMFVPKGYVFVLGDNRNKSFDSHNWGPLPIENIVGRSVFRYWPPSKVSD 330

Query: 361 -IMSDQ 363
            I  DQ
Sbjct: 361 TIYHDQ 330

BLAST of Cmc07g0184481 vs. TAIR 10
Match: AT3G24590.1 (plastidic type i signal peptidase 1 )

HSP 1 Score: 255.0 bits (650), Expect = 9.4e-68
Identity = 119/180 (66.11%), Postives = 147/180 (81.67%), Query Frame = 0

Query: 177 EKSSWISRFLNNCSEDAKAIATALTVSVLFRSSLAEPRSIPSSSMYPTLDVGDRILAEKV 236
           EK+     +L+  S+DA+ +  A+ VS+ FR  +AEPR IPS SMYPT DVGDR++AEKV
Sbjct: 99  EKNRLFPEWLDFTSDDAQTVFVAIAVSLAFRYFIAEPRYIPSLSMYPTFDVGDRLVAEKV 158

Query: 237 SYFFRKPSVSDIVIFKAPPILQKIGYKSNDVFIKRIVAKAGDCVEVRDGKLLVNGVAQNE 296
           SY+FRKP  +DIVIFK+PP+LQ++GY   DVFIKRIVAK GD VEV +GKL+VNGVA+NE
Sbjct: 159 SYYFRKPCANDIVIFKSPPVLQEVGYTDADVFIKRIVAKEGDLVEVHNGKLMVNGVARNE 218

Query: 297 KFILEPLSYNMDPVLVPEGYVFVLGDNRNNSFDSHNWGPLPVENIVGRSVFRYWPPSKVS 356
           KFILEP  Y M P+ VPE  VFV+GDNRNNS+DSH WGPLP++NI+GRSVFRYWPP++VS
Sbjct: 219 KFILEPPGYEMTPIRVPENSVFVMGDNRNNSYDSHVWGPLPLKNIIGRSVFRYWPPNRVS 278

BLAST of Cmc07g0184481 vs. TAIR 10
Match: AT1G23465.1 (Peptidase S24/S26A/S26B/S26C family protein )

HSP 1 Score: 78.6 bits (192), Expect = 1.2e-14
Identity = 48/131 (36.64%), Postives = 72/131 (54.96%), Query Frame = 0

Query: 220 SMYPTL-DVGDRILAEKVSYFFRKPSVSDIVIFKAPPILQKIGYKSNDVFIKRIVAKAGD 279
           SM PTL   G+ +LAE++S  ++KPS  DIV+ ++P          N   IKR+V   GD
Sbjct: 47  SMIPTLHPSGNMLLAERISKRYQKPSRGDIVVIRSPE-------NPNKTPIKRVVGVEGD 106

Query: 280 CVEVRDGKLLVNGVAQNEKFILEPL-SYNMDPVLVPEGYVFVLGDNRNNSFDSHNWGPLP 339
           C+                 F+++P+ S     ++VP+G+VFV GD  +NS DS N+GP+P
Sbjct: 107 CI----------------SFVIDPVKSDESQTIVVPKGHVFVQGDYTHNSRDSRNFGPVP 154

Query: 340 VENIVGRSVFR 349
              I GR ++R
Sbjct: 167 YGLIQGRVLWR 154

BLAST of Cmc07g0184481 vs. TAIR 10
Match: AT1G29960.1 (Peptidase S24/S26A/S26B/S26C family protein )

HSP 1 Score: 76.3 bits (186), Expect = 6.0e-14
Identity = 47/134 (35.07%), Postives = 71/134 (52.99%), Query Frame = 0

Query: 220 SMYPTL-DVGDRILAEKVSYFFRKPSVSDIVIFKAPPILQKIGYKSNDVFIKRIVAKAGD 279
           SM PTL   G+ +LAE++S  ++KPS  DIV+ ++P          N   IKR++   GD
Sbjct: 47  SMTPTLHPSGNVLLAERISKRYQKPSRGDIVVIRSPE-------NPNKTPIKRVIGIEGD 106

Query: 280 CVEVRDGKLLVNGVAQNEKFILEP-LSYNMDPVLVPEGYVFVLGDNRNNSFDSHNWGPLP 339
           C+                 F+++   S     ++VP+G+VFV GD  +NS DS N+G +P
Sbjct: 107 CI----------------SFVIDSRKSDESQTIVVPKGHVFVQGDYTHNSRDSRNFGTVP 157

Query: 340 VENIVGRSVFRYWP 352
              I GR ++R WP
Sbjct: 167 YGLIQGRVLWRVWP 157

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_008452489.11.1e-208100.00PREDICTED: thylakoidal processing peptidase 1, chloroplastic-like [Cucumis melo]... [more]
XP_011654082.11.8e-20196.77thylakoidal processing peptidase 1, chloroplastic [Cucumis sativus] >KGN55154.1 ... [more]
XP_038900232.12.0e-19292.18thylakoidal processing peptidase 1, chloroplastic-like [Benincasa hispida][more]
XP_023536434.15.1e-18588.41thylakoidal processing peptidase 1, chloroplastic-like [Cucurbita pepo subsp. pe... [more]
KAG6591353.11.5e-18488.14putative thylakoidal processing peptidase 2, chloroplastic, partial [Cucurbita a... [more]
Match NameE-valueIdentityDescription
Q9M9Z22.6e-9953.99Probable thylakoidal processing peptidase 2, chloroplastic OS=Arabidopsis thalia... [more]
O043481.3e-9856.56Thylakoidal processing peptidase 1, chloroplastic OS=Arabidopsis thaliana OX=370... [more]
Q8H0W11.3e-6666.11Chloroplast processing peptidase OS=Arabidopsis thaliana OX=3702 GN=PLSP1 PE=2 S... [more]
P726602.5e-4150.00Probable signal peptidase I-1 OS=Synechocystis sp. (strain PCC 6803 / Kazusa) OX... [more]
Q518761.6e-3541.67Signal peptidase I OS=Phormidium laminosum OX=32059 GN=lepB PE=3 SV=1[more]
Match NameE-valueIdentityDescription
A0A5A7V7L75.5e-209100.00Thylakoidal processing peptidase 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E56... [more]
A0A1S3BTW25.5e-209100.00thylakoidal processing peptidase 1, chloroplastic-like OS=Cucumis melo OX=3656 G... [more]
A0A0A0L2908.5e-20296.77Peptidase_S26 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G6384... [more]
A0A6J1FC761.2e-18488.14thylakoidal processing peptidase 1, chloroplastic-like OS=Cucurbita moschata OX=... [more]
A0A6J1IF811.0e-18387.60thylakoidal processing peptidase 1, chloroplastic-like OS=Cucurbita maxima OX=36... [more]
Match NameE-valueIdentityDescription
AT1G06870.11.9e-10053.99Peptidase S24/S26A/S26B/S26C family protein [more]
AT2G30440.19.3e-10056.56thylakoid processing peptide [more]
AT3G24590.19.4e-6866.11plastidic type i signal peptidase 1 [more]
AT1G23465.11.2e-1436.64Peptidase S24/S26A/S26B/S26C family protein [more]
AT1G29960.16.0e-1435.07Peptidase S24/S26A/S26B/S26C family protein [more]
InterPro
Analysis Name: InterPro Annotations of Melon (Charmono) v1.1
Date Performed: 2022-10-13
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000223Peptidase S26A, signal peptidase IPRINTSPR00727LEADERPTASEcoord: 268..280
score: 46.15
coord: 312..331
score: 60.12
coord: 209..225
score: 48.18
IPR000223Peptidase S26A, signal peptidase ITIGRFAMTIGR02227TIGR02227coord: 194..351
e-value: 1.1E-38
score: 130.6
IPR000223Peptidase S26A, signal peptidase IPANTHERPTHR43390SIGNAL PEPTIDASE Icoord: 1..368
IPR019533Peptidase S26PFAMPF10502Peptidase_S26coord: 193..350
e-value: 2.7E-42
score: 144.6
IPR019533Peptidase S26CDDcd06530S26_SPase_Icoord: 212..344
e-value: 1.03876E-26
score: 99.5823
NoneNo IPR availableGENE3D2.10.109.10Umud Fragment, subunit Acoord: 206..366
e-value: 1.2E-35
score: 124.3
NoneNo IPR availablePANTHERPTHR43390:SF2THYLAKOIDAL PROCESSING PEPTIDASE 2, CHLOROPLASTIC-RELATEDcoord: 1..368
IPR019756Peptidase S26A, signal peptidase I, serine active sitePROSITEPS00501SPASE_I_1coord: 218..225
IPR019758Peptidase S26A, signal peptidase I, conserved sitePROSITEPS00761SPASE_I_3coord: 317..330
IPR036286LexA/Signal peptidase-like superfamilySUPERFAMILY51306LexA/Signal peptidasecoord: 207..357

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cmc07g0184481.1Cmc07g0184481.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006465 signal peptide processing
biological_process GO:0010027 thylakoid membrane organization
biological_process GO:0006508 proteolysis
cellular_component GO:0009535 chloroplast thylakoid membrane
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0016020 membrane
molecular_function GO:0004252 serine-type endopeptidase activity
molecular_function GO:0008236 serine-type peptidase activity