Cmc07g0183611 (gene) Melon (Charmono) v1.1

Overview
NameCmc07g0183611
Typegene
OrganismCucumis melo L. var. cantalupensis cv. Charmono (Melon (Charmono) v1.1)
DescriptionCytochrome P450
LocationCMiso1.1chr07: 1998772 .. 2000835 (+)
RNA-Seq ExpressionCmc07g0183611
SyntenyCmc07g0183611
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AAGTATCACACCACACTAAGAATTCATAAATAATTCAAACAATTCTCATAGAATTTCCATCCCAATATGGATATGGAACAACAAACTTCTTTCTTCTTCTTCTTCTCTCATTTCAATTTCCCTCTCTTAGCCATTTTCTTAACAACTCTCTTTCTCATTTCCAAATTCCGTAGATCAGAGCACACCAAACTGCTGCTCCCACCAAGTCCTCCTTCTCTTCCTGTAATCGGTCACCTTCATCTTCTCAAAGAACCATTCCACCAAATGTTACAAGATCTCTCTCTCAAATACGGCCCTGTTTTCTCCCTCACTCTCGGTTTCCGCCCCCTCGTGGTCGTCTCCTCCCCTTCCGCCGTACACGAATGCTTCACAAAAAACGACATCGTTTTCGCCAGCCGCCCCCGAATGCTGTCCGGAAAGATCCTCAATTACGATTACACAGCCGTTGGTGCCACCGCGTACGGGCAGCACTGGCGTATCATGCGACGGATAGCTACCACCGAATTGCTGTCCACCAAGCGTCTCAACTACTTTAGTAAACTCAGAGAAGAGGAGGTGAAACTGTGGGTGAAAAGGTTGTACGGATCAGTGACAATCAGGGAAGTTCAAAAGGGAGGTAGAGTGGGATTAAGGTCGAAATTGACGGAGCTTTCGTTCAATATTGCGTTGATGTTAATGGCGGGAAGAAGGTATTTTGGAGAGGAAGTGGAAGAGGGGGAGAGTGAGGAGGCGAAGGAATTGAAGGAGACGATGAAGCAAGTTCCTCTTTTGAGTGGAGCTTCGTATCCGGCAGATTTTTTGTCGGTATTGAAATTGATTGATTTTCAAGGATTCAGGAAGAGATTGGCGAAGGTGAGAGTGGATGGAGATGCATTTGCTCAAAGTCTGGTCGATGAAAGACGGGAATTGAAGGGTTCTTGGTGTGAGAGGAAGACCATGATTGATGCTATGTTGTCGTTGCAGGAATCAGACCCTGAGTATTATAAGGATGATATTATTAAGGGACAATTCTTGGTAATTAAGCTTAATTATATCTAACTTACCTTAAATTAATTAATATTCCCATCTCATATTAACATTATTTATTTGTATTGAATTTGAATACTCTAATTAATTAAAGCAATGTTATGTTTGCAGACTTTACTAGCTGCAGGAACTGATACAATCGCAAGCACGTTAGAATGGGCAATGTCACTACTCCTAAACCATCCTATCGTAATGAACAAGGCTTGGACAGAGATAAACCAGGTTGTTGGAGACCAAAGACTAGTAAGGGAAGCTGATACAAACAACCTACACTATCTTCAATCCATAATCAACGAGACCTACCGTCTATTTCCAGCCCTTCCAATACTGGTGCCACGTGAATCATCAGAAGATTGCACAATTGGAGGGTTCGATATTCCAAAAGGAACCATGTTGGTGGTGAATGCTTGGGCTGTCCATCGAAACCCCGAGGTTTGGGAAGATCCTAATTGTTTTAAACCAGAAAGGTTTAAACAAGGGGAAACTGTGGAGGTCAGTAAATTGTTGCCTTTCGGAATGGGACGAAGGGCTTGTCCAGGGGCAGGGCTTGCTCATAGGGTGGTGTCTTTGGCTTTGGCTACTCTTATTCAATGCTTTGAATGGGGCAAACTCGATGACAAAAAAGATGTTGACTTGTGCCCAGGGATTGGGCTCACTATGCCTAAAGCTATTCCTCTTGAAGCCATGTGCAAACCTCGTGCTTCCATCACTCATTTACTATCACAGCTTTGATGCCATGACCTCTCGTTTATCTGAATACTATGTGTTTTGTATTTTTAATTTGTATTTTATCTCATAATTAGCCCAAGCATGAAATTGGGCATTATGTTTGTTATTGTTGCAACTTAGTTTTGGTCCCAAGCATGAAAGTGCTTTAAGTTGTTGGATATATCTGTGTCCATGTTGTAGTTAAGGTCTGCGTTCTGGTTTCACATGAAATCTAGAACGAAATAATGTAAAATCTTGCCTATTCGTTTTTTTATTTTATATTATGATAATTATTATTGCAAATATAGCTTAAATTATAAAGTATGATGA

mRNA sequence

AAGTATCACACCACACTAAGAATTCATAAATAATTCAAACAATTCTCATAGAATTTCCATCCCAATATGGATATGGAACAACAAACTTCTTTCTTCTTCTTCTTCTCTCATTTCAATTTCCCTCTCTTAGCCATTTTCTTAACAACTCTCTTTCTCATTTCCAAATTCCGTAGATCAGAGCACACCAAACTGCTGCTCCCACCAAGTCCTCCTTCTCTTCCTGTAATCGGTCACCTTCATCTTCTCAAAGAACCATTCCACCAAATGTTACAAGATCTCTCTCTCAAATACGGCCCTGTTTTCTCCCTCACTCTCGGTTTCCGCCCCCTCGTGGTCGTCTCCTCCCCTTCCGCCGTACACGAATGCTTCACAAAAAACGACATCGTTTTCGCCAGCCGCCCCCGAATGCTGTCCGGAAAGATCCTCAATTACGATTACACAGCCGTTGGTGCCACCGCGTACGGGCAGCACTGGCGTATCATGCGACGGATAGCTACCACCGAATTGCTGTCCACCAAGCGTCTCAACTACTTTAGTAAACTCAGAGAAGAGGAGGTGAAACTGTGGGTGAAAAGGTTGTACGGATCAGTGACAATCAGGGAAGTTCAAAAGGGAGGTAGAGTGGGATTAAGGTCGAAATTGACGGAGCTTTCGTTCAATATTGCGTTGATGTTAATGGCGGGAAGAAGGTATTTTGGAGAGGAAGTGGAAGAGGGGGAGAGTGAGGAGGCGAAGGAATTGAAGGAGACGATGAAGCAAGTTCCTCTTTTGAGTGGAGCTTCGTATCCGGCAGATTTTTTGTCGGTATTGAAATTGATTGATTTTCAAGGATTCAGGAAGAGATTGGCGAAGGTGAGAGTGGATGGAGATGCATTTGCTCAAAGTCTGGTCGATGAAAGACGGGAATTGAAGGGTTCTTGGTGTGAGAGGAAGACCATGATTGATGCTATGTTGTCGTTGCAGGAATCAGACCCTGAGTATTATAAGGATGATATTATTAAGGGACAATTCTTGACTTTACTAGCTGCAGGAACTGATACAATCGCAAGCACGTTAGAATGGGCAATGTCACTACTCCTAAACCATCCTATCGTAATGAACAAGGCTTGGACAGAGATAAACCAGGTTGTTGGAGACCAAAGACTAGTAAGGGAAGCTGATACAAACAACCTACACTATCTTCAATCCATAATCAACGAGACCTACCGTCTATTTCCAGCCCTTCCAATACTGGTGCCACGTGAATCATCAGAAGATTGCACAATTGGAGGGTTCGATATTCCAAAAGGAACCATGTTGGTGGTGAATGCTTGGGCTGTCCATCGAAACCCCGAGGTTTGGGAAGATCCTAATTGTTTTAAACCAGAAAGGTTTAAACAAGGGGAAACTGTGGAGGTCAGTAAATTGTTGCCTTTCGGAATGGGACGAAGGGCTTGTCCAGGGGCAGGGCTTGCTCATAGGGTGGTGTCTTTGGCTTTGGCTACTCTTATTCAATGCTTTGAATGGGGCAAACTCGATGACAAAAAAGATGTTGACTTGTGCCCAGGGATTGGGCTCACTATGCCTAAAGCTATTCCTCTTGAAGCCATGTGCAAACCTCGTGCTTCCATCACTCATTTACTATCACAGCTTTGATGCCATGACCTCTCGTTTATCTGAATACTATGTGTTTTGTATTTTTAATTTGTATTTTATCTCATAATTAGCCCAAGCATGAAATTGGGCATTATGTTTGTTATTGTTGCAACTTAGTTTTGGTCCCAAGCATGAAAGTGCTTTAAGTTGTTGGATATATCTGTGTCCATGTTGTAGTTAAGGTCTGCGTTCTGGTTTCACATGAAATCTAGAACGAAATAATGTAAAATCTTGCCTATTCGTTTTTTTATTTTATATTATGATAATTATTATTGCAAATATAGCTTAAATTATAAAGTATGATGA

Coding sequence (CDS)

ATGGATATGGAACAACAAACTTCTTTCTTCTTCTTCTTCTCTCATTTCAATTTCCCTCTCTTAGCCATTTTCTTAACAACTCTCTTTCTCATTTCCAAATTCCGTAGATCAGAGCACACCAAACTGCTGCTCCCACCAAGTCCTCCTTCTCTTCCTGTAATCGGTCACCTTCATCTTCTCAAAGAACCATTCCACCAAATGTTACAAGATCTCTCTCTCAAATACGGCCCTGTTTTCTCCCTCACTCTCGGTTTCCGCCCCCTCGTGGTCGTCTCCTCCCCTTCCGCCGTACACGAATGCTTCACAAAAAACGACATCGTTTTCGCCAGCCGCCCCCGAATGCTGTCCGGAAAGATCCTCAATTACGATTACACAGCCGTTGGTGCCACCGCGTACGGGCAGCACTGGCGTATCATGCGACGGATAGCTACCACCGAATTGCTGTCCACCAAGCGTCTCAACTACTTTAGTAAACTCAGAGAAGAGGAGGTGAAACTGTGGGTGAAAAGGTTGTACGGATCAGTGACAATCAGGGAAGTTCAAAAGGGAGGTAGAGTGGGATTAAGGTCGAAATTGACGGAGCTTTCGTTCAATATTGCGTTGATGTTAATGGCGGGAAGAAGGTATTTTGGAGAGGAAGTGGAAGAGGGGGAGAGTGAGGAGGCGAAGGAATTGAAGGAGACGATGAAGCAAGTTCCTCTTTTGAGTGGAGCTTCGTATCCGGCAGATTTTTTGTCGGTATTGAAATTGATTGATTTTCAAGGATTCAGGAAGAGATTGGCGAAGGTGAGAGTGGATGGAGATGCATTTGCTCAAAGTCTGGTCGATGAAAGACGGGAATTGAAGGGTTCTTGGTGTGAGAGGAAGACCATGATTGATGCTATGTTGTCGTTGCAGGAATCAGACCCTGAGTATTATAAGGATGATATTATTAAGGGACAATTCTTGACTTTACTAGCTGCAGGAACTGATACAATCGCAAGCACGTTAGAATGGGCAATGTCACTACTCCTAAACCATCCTATCGTAATGAACAAGGCTTGGACAGAGATAAACCAGGTTGTTGGAGACCAAAGACTAGTAAGGGAAGCTGATACAAACAACCTACACTATCTTCAATCCATAATCAACGAGACCTACCGTCTATTTCCAGCCCTTCCAATACTGGTGCCACGTGAATCATCAGAAGATTGCACAATTGGAGGGTTCGATATTCCAAAAGGAACCATGTTGGTGGTGAATGCTTGGGCTGTCCATCGAAACCCCGAGGTTTGGGAAGATCCTAATTGTTTTAAACCAGAAAGGTTTAAACAAGGGGAAACTGTGGAGGTCAGTAAATTGTTGCCTTTCGGAATGGGACGAAGGGCTTGTCCAGGGGCAGGGCTTGCTCATAGGGTGGTGTCTTTGGCTTTGGCTACTCTTATTCAATGCTTTGAATGGGGCAAACTCGATGACAAAAAAGATGTTGACTTGTGCCCAGGGATTGGGCTCACTATGCCTAAAGCTATTCCTCTTGAAGCCATGTGCAAACCTCGTGCTTCCATCACTCATTTACTATCACAGCTTTGA

Protein sequence

MDMEQQTSFFFFFSHFNFPLLAIFLTTLFLISKFRRSEHTKLLLPPSPPSLPVIGHLHLLKEPFHQMLQDLSLKYGPVFSLTLGFRPLVVVSSPSAVHECFTKNDIVFASRPRMLSGKILNYDYTAVGATAYGQHWRIMRRIATTELLSTKRLNYFSKLREEEVKLWVKRLYGSVTIREVQKGGRVGLRSKLTELSFNIALMLMAGRRYFGEEVEEGESEEAKELKETMKQVPLLSGASYPADFLSVLKLIDFQGFRKRLAKVRVDGDAFAQSLVDERRELKGSWCERKTMIDAMLSLQESDPEYYKDDIIKGQFLTLLAAGTDTIASTLEWAMSLLLNHPIVMNKAWTEINQVVGDQRLVREADTNNLHYLQSIINETYRLFPALPILVPRESSEDCTIGGFDIPKGTMLVVNAWAVHRNPEVWEDPNCFKPERFKQGETVEVSKLLPFGMGRRACPGAGLAHRVVSLALATLIQCFEWGKLDDKKDVDLCPGIGLTMPKAIPLEAMCKPRASITHLLSQL
Homology
BLAST of Cmc07g0183611 vs. NCBI nr
Match: XP_008452612.1 (PREDICTED: isoflavone 2'-hydroxylase-like [Cucumis melo] >KAA0064425.1 isoflavone 2'-hydroxylase-like [Cucumis melo var. makuwa] >TYK20162.1 isoflavone 2'-hydroxylase-like [Cucumis melo var. makuwa])

HSP 1 Score: 1039.6 bits (2687), Expect = 8.8e-300
Identity = 518/522 (99.23%), Postives = 520/522 (99.62%), Query Frame = 0

Query: 1   MDMEQQTSFFFFFSHFNFPLLAIFLTTLFLISKFRRSEHTKLLLPPSPPSLPVIGHLHLL 60
           MDMEQQTSFFFFFSHFNFPLLAIFLTTLFLISKFRRSEHTKLLLPPSPPSLPVIGHLHLL
Sbjct: 1   MDMEQQTSFFFFFSHFNFPLLAIFLTTLFLISKFRRSEHTKLLLPPSPPSLPVIGHLHLL 60

Query: 61  KEPFHQMLQDLSLKYGPVFSLTLGFRPLVVVSSPSAVHECFTKNDIVFASRPRMLSGKIL 120
           KEPFHQMLQDLSLKYGPVFSLTLGFRPLVVVSSPSAVHECFTKNDIVFASRPRMLSGKIL
Sbjct: 61  KEPFHQMLQDLSLKYGPVFSLTLGFRPLVVVSSPSAVHECFTKNDIVFASRPRMLSGKIL 120

Query: 121 NYDYTAVGATAYGQHWRIMRRIATTELLSTKRLNYFSKLREEEVKLWVKRLYGSVTIREV 180
           NYDYTAVGATAYGQHWRIMRRIATTELLSTKRLN FSKLREEEVKLWVKRLYGSVTIREV
Sbjct: 121 NYDYTAVGATAYGQHWRIMRRIATTELLSTKRLNDFSKLREEEVKLWVKRLYGSVTIREV 180

Query: 181 QKGGRVGLRSKLTELSFNIALMLMAGRRYFGEEVEEGESEEAKELKETMKQVPLLSGASY 240
           QKGGRVGLRSKLTELSFNIALML AGRRYFGEEVEEGESEEAKELKETMKQVPLLSGASY
Sbjct: 181 QKGGRVGLRSKLTELSFNIALMLTAGRRYFGEEVEEGESEEAKELKETMKQVPLLSGASY 240

Query: 241 PADFLSVLKLIDFQGFRKRLAKVRVDGDAFAQSLVDERRELKGSWCERKTMIDAMLSLQE 300
           PADFLSVLKLIDFQGFRKRLAKVRVDGDAFAQSLVDERRELKGSWCERKTMIDAMLSLQE
Sbjct: 241 PADFLSVLKLIDFQGFRKRLAKVRVDGDAFAQSLVDERRELKGSWCERKTMIDAMLSLQE 300

Query: 301 SDPEYYKDDIIKGQFLTLLAAGTDTIASTLEWAMSLLLNHPIVMNKAWTEINQVVGDQRL 360
           SDPEYYKDDIIKGQFLTLLAAGTDTIASTLEWAMSLLLNHPIVMNKAWTEINQVVGDQRL
Sbjct: 301 SDPEYYKDDIIKGQFLTLLAAGTDTIASTLEWAMSLLLNHPIVMNKAWTEINQVVGDQRL 360

Query: 361 VREADTNNLHYLQSIINETYRLFPALPILVPRESSEDCTIGGFDIPKGTMLVVNAWAVHR 420
           VREADTNNLHYLQSIINETYRLFPALPILVPRESSEDCTIGGFDIPKGTMLVVNAWAVHR
Sbjct: 361 VREADTNNLHYLQSIINETYRLFPALPILVPRESSEDCTIGGFDIPKGTMLVVNAWAVHR 420

Query: 421 NPEVWEDPNCFKPERFKQGETVEVSKLLPFGMGRRACPGAGLAHRVVSLALATLIQCFEW 480
           NPEVWEDPNCFKPERFKQGETVEVSKLLPFGMGRRACPGAGLAHRVVSLALATLIQCFEW
Sbjct: 421 NPEVWEDPNCFKPERFKQGETVEVSKLLPFGMGRRACPGAGLAHRVVSLALATLIQCFEW 480

Query: 481 GKLDDKKDVDLCPGIGLTMPKAIPLEAMCKPRASITHLLSQL 523
           GKLD+KKDVDLCPGIGLTMPKAIPLEAMCKPRAS+THLLSQL
Sbjct: 481 GKLDNKKDVDLCPGIGLTMPKAIPLEAMCKPRASVTHLLSQL 522

BLAST of Cmc07g0183611 vs. NCBI nr
Match: XP_004141394.1 (cytochrome P450 81Q32 [Cucumis sativus] >KGN55244.1 hypothetical protein Csa_012467 [Cucumis sativus])

HSP 1 Score: 963.8 bits (2490), Expect = 6.2e-277
Identity = 482/524 (91.98%), Postives = 499/524 (95.23%), Query Frame = 0

Query: 3   MEQQTSFFFFFSH----FNFPLLAIFLTTLFLISKFRRSEHTKLLLPPSPPSLPVIGHLH 62
           MEQQTSFFF   H     + PLLAIFLT+LFLISKFRR++HTK LLPPSPPSLPVIGHLH
Sbjct: 1   MEQQTSFFFSQDHHFHPISIPLLAIFLTSLFLISKFRRTQHTK-LLPPSPPSLPVIGHLH 60

Query: 63  LLKEPFHQMLQDLSLKYGPVFSLTLGFRPLVVVSSPSAVHECFTKNDIVFASRPRMLSGK 122
           LLKEPFHQMLQD S KYGPVFSLTLGFRPLVVVSSPSAV ECFTKNDIVFASRPRMLSGK
Sbjct: 61  LLKEPFHQMLQDFSKKYGPVFSLTLGFRPLVVVSSPSAVQECFTKNDIVFASRPRMLSGK 120

Query: 123 ILNYDYTAVGATAYGQHWRIMRRIATTELLSTKRLNYFSKLREEEVKLWVKRLYGSVTIR 182
           ILNYDYTAVGATAYGQHWRIMRRIATTELLSTKRLN  SK+REEEVKLWVKRLYGSVT  
Sbjct: 121 ILNYDYTAVGATAYGQHWRIMRRIATTELLSTKRLNDLSKIREEEVKLWVKRLYGSVTRA 180

Query: 183 EVQKGGRVGLRSKLTELSFNIALMLMAGRRYFGEEVEEGESEEAKELKETMKQVPLLSGA 242
           EV KGGRVGLRSKLTELSFNIALMLMAGRRYFG+E+E GESEEAKELKETMKQVPLLSGA
Sbjct: 181 EVNKGGRVGLRSKLTELSFNIALMLMAGRRYFGDEMEAGESEEAKELKETMKQVPLLSGA 240

Query: 243 SYPADFLSVLKLIDFQGFRKRLAKVRVDGDAFAQSLVDERRELKGSWCERKTMIDAMLSL 302
           SYPADFLSVLKLIDFQGFRKRLAKVRVDGDAFAQSLVDERR+LKGSWCERKTMID+ML L
Sbjct: 241 SYPADFLSVLKLIDFQGFRKRLAKVRVDGDAFAQSLVDERRKLKGSWCERKTMIDSMLCL 300

Query: 303 QESDPEYYKDDIIKGQFLTLLAAGTDTIASTLEWAMSLLLNHPIVMNKAWTEINQVVGDQ 362
           QESDPEYYKDDIIKGQFLTLLAAGTDT ASTLEWAMSLLLNHP V+ KAWTEINQVVGDQ
Sbjct: 301 QESDPEYYKDDIIKGQFLTLLAAGTDTTASTLEWAMSLLLNHPTVLEKAWTEINQVVGDQ 360

Query: 363 RLVREADTNNLHYLQSIINETYRLFPALPILVPRESSEDCTIGGFDIPKGTMLVVNAWAV 422
           RL+READTN LHYLQ+IINETYRLFPALPILVPRESSEDCTIGGFDIPKGTMLVVNAWAV
Sbjct: 361 RLIREADTNKLHYLQAIINETYRLFPALPILVPRESSEDCTIGGFDIPKGTMLVVNAWAV 420

Query: 423 HRNPEVWEDPNCFKPERFKQGETVEVSKLLPFGMGRRACPGAGLAHRVVSLALATLIQCF 482
           HR+P VWEDPNCFKPERFKQGETVEV+KLLPFGMGRRACPGAGLAHRVVSLALATLIQCF
Sbjct: 421 HRDPNVWEDPNCFKPERFKQGETVEVNKLLPFGMGRRACPGAGLAHRVVSLALATLIQCF 480

Query: 483 EWGKLDDKKDVDLCPGIGLTMPKAIPLEAMCKPRASITHLLSQL 523
           EWGKLD++KDVDLCPGIGLTMPKAIPLEAMCKPRAS+THLLSQL
Sbjct: 481 EWGKLDNQKDVDLCPGIGLTMPKAIPLEAMCKPRASVTHLLSQL 523

BLAST of Cmc07g0183611 vs. NCBI nr
Match: XP_038900261.1 (LOW QUALITY PROTEIN: cytochrome P450 81Q32-like [Benincasa hispida])

HSP 1 Score: 798.1 bits (2060), Expect = 4.5e-227
Identity = 417/521 (80.04%), Postives = 446/521 (85.60%), Query Frame = 0

Query: 3   MEQQTSFFFFFSHF-NFPLLAIFLTTLFLISKFRRSEHTKLLLPPSPPSLPVIGHLHLLK 62
           ME+Q S  FFFSHF   PL A+ +TTLFL+SKFR++EH K  LPPSPPSLPVIGHLHLLK
Sbjct: 1   MEEQAS--FFFSHFPTIPLFALLITTLFLLSKFRKTEHRK--LPPSPPSLPVIGHLHLLK 60

Query: 63  EPFHQMLQDLSLKYGPVFSLTLGFRPLVVVSSPSAVHECFTKNDIVFASRPRMLSGKILN 122
           EPFHQMLQDLS KYGPVFSLTLGFRP+VVVS+PSAV E                  KILN
Sbjct: 61  EPFHQMLQDLSKKYGPVFSLTLGFRPVVVVSAPSAVQE-----------------WKILN 120

Query: 123 YDYTAVGATAYGQHWRIMRRIATTELLSTKRLNYFSKLREEEVKLWVKRLYGSVTIREVQ 182
           YDYTAVGATAYGQHWRIMRRIATTELLST RLN  SK+REEEVKLWVKRLYG   + E  
Sbjct: 121 YDYTAVGATAYGQHWRIMRRIATTELLSTHRLNSLSKIREEEVKLWVKRLYGMTEVGESN 180

Query: 183 KGGRVGLRSKLTELSFNIALMLMAGRRYFGEEVEEGESEEAKELKETMKQVPLLSGASYP 242
           KG RVGLRSKLTELSFNIALMLM GRRYFG EV EGESEEAKELKETMKQVPLLSGASYP
Sbjct: 181 KGVRVGLRSKLTELSFNIALMLMTGRRYFGGEV-EGESEEAKELKETMKQVPLLSGASYP 240

Query: 243 ADFLSVLKLIDFQGFRKRLAKVRVDGDAFAQSLVDERRELKGSWCERKTMIDAMLSLQES 302
           ADFLSVLK +DFQ        VRVDGDAFAQS+VDERR+LKGS CERKT+ID++L LQES
Sbjct: 241 ADFLSVLKWVDFQ--------VRVDGDAFAQSVVDERRKLKGS-CERKTIIDSLLLLQES 300

Query: 303 DPEYYKDDIIKGQFLTLLAAGTDTIASTLEWAMSLLLNHPIVMNKAWTEINQVVGDQRLV 362
           DPEYYKD+IIKGQFLTLLAAGTDT A TLEWAMSLLLNHP  M KAWTEIN+VVGDQRLV
Sbjct: 301 DPEYYKDEIIKGQFLTLLAAGTDTTARTLEWAMSLLLNHPTEMKKAWTEINEVVGDQRLV 360

Query: 363 READTNNLHYLQSIINETYRLFPALPILVPRESSEDCTIGGFDIPKGTMLVVNAWAVHRN 422
            EAD N  HYLQ+IINETYRLFPALPIL PRESSEDCTIGGFDIPKGTMLVVNAWAVHR+
Sbjct: 361 EEADRNKPHYLQAIINETYRLFPALPILAPRESSEDCTIGGFDIPKGTMLVVNAWAVHRD 420

Query: 423 PEVWEDPNCFKPERFKQGETVEVSKLLPFGMGRRACPGAGLAHRVVSLALATLIQCFEWG 482
           P VWE PN F+PERFK G+ +EVSKLLPFGMGRRA PGAGLAHRVV+LALATLIQCFEWG
Sbjct: 421 PNVWEHPNSFQPERFKPGQXLEVSKLLPFGMGRRAGPGAGLAHRVVALALATLIQCFEWG 480

Query: 483 KLDDKKDVDLCPGIGLTMPKAIPLEAMCKPRASITHLLSQL 523
           +  D+K +DL PGIGLTMP+AIPLEA+CKPRASITHLLSQL
Sbjct: 481 R-PDQKGLDLSPGIGLTMPRAIPLEAICKPRASITHLLSQL 489

BLAST of Cmc07g0183611 vs. NCBI nr
Match: XP_022940149.1 (cytochrome P450 81E8-like [Cucurbita moschata])

HSP 1 Score: 794.7 bits (2051), Expect = 5.0e-226
Identity = 400/510 (78.43%), Postives = 442/510 (86.67%), Query Frame = 0

Query: 13  FSHFNFPLLAIFLTTLFLISKFRRSEHTKLLLPPSPPSLPVIGHLHLLKEPFHQMLQDLS 72
           FS+F  PLL + L  L LI KFR++EH    LPP PPSLPVIGHLHLLKEPFHQMLQDLS
Sbjct: 11  FSNFEVPLLVLIL-ALLLIFKFRKAEHKN--LPPGPPSLPVIGHLHLLKEPFHQMLQDLS 70

Query: 73  LKYGPVFSLTLGFRPLVVVSSPSAVHECFTKNDIVFASRPRMLSGKILNYDYTAVGATAY 132
            KYGP+ SL+LGFRP+VVVSSP+A  ECF+KNDI+FASRPRM+SGKILNYDYTAVGATAY
Sbjct: 71  QKYGPILSLSLGFRPVVVVSSPAAAQECFSKNDIIFASRPRMISGKILNYDYTAVGATAY 130

Query: 133 GQHWRIMRRIATTELLSTKRLNYFSKLREEEVKLWVKRLYGSVTIREVQKGGRVGLRSKL 192
           GQHWRIMRRIATTELLST RLN    +R++EVKLW+K LY       V    RV +RSKL
Sbjct: 131 GQHWRIMRRIATTELLSTYRLNALINIRQQEVKLWLKSLYRISGKNNV----RVEIRSKL 190

Query: 193 TELSFNIALMLMAGRRYFGEEVEEGESEEAKELKETMKQVPLLSGASYPADFLSVLKLID 252
           TELSFNIALMLM G+RYFG+E  + +SEEA+ELK TMKQVPLLSGASYPADFLSVLKL+D
Sbjct: 191 TELSFNIALMLMVGKRYFGDEAVD-DSEEARELKNTMKQVPLLSGASYPADFLSVLKLVD 250

Query: 253 FQGFRKRLAKVRVDGDAFAQSLVDERRELKGSWCERKTMIDAMLSLQESDPEYYKDDIIK 312
           +QGFRKRLAKVRVDGDAFAQSL+DERR  K    ERKTMID++LS+Q+ +PEYYKDDIIK
Sbjct: 251 YQGFRKRLAKVRVDGDAFAQSLIDERRNKKADDNERKTMIDSLLSMQQMEPEYYKDDIIK 310

Query: 313 GQFLTLLAAGTDTIASTLEWAMSLLLNHPIVMNKAWTEINQVVGDQRLVREADTNNLHYL 372
           GQFLTLLAAGTDT A TLEWA+SLLLNHP  M KAWTEIN+ VGD+RLV E D N LHYL
Sbjct: 311 GQFLTLLAAGTDTTAGTLEWALSLLLNHPTAMKKAWTEINEHVGDERLVEEMDLNQLHYL 370

Query: 373 QSIINETYRLFPALPILVPRESSEDCTIGGFDIPKGTMLVVNAWAVHRNPEVWEDPNCFK 432
           Q IINETYRLFPALPILVPRESSEDCTIGGFD+PKGTMLVVNAWA+HR+P+VWEDPN F+
Sbjct: 371 QGIINETYRLFPALPILVPRESSEDCTIGGFDVPKGTMLVVNAWAIHRDPKVWEDPNSFR 430

Query: 433 PERFKQGETVEVSKLLPFGMGRRACPGAGLAHRVVSLALATLIQCFEWGKLDDKKDVDLC 492
           PERFK+GE+VEVSKLLPFGMGRRACPGAGLAHRVV LALATLIQCFEW +LD+ K+VDL 
Sbjct: 431 PERFKEGESVEVSKLLPFGMGRRACPGAGLAHRVVGLALATLIQCFEWERLDN-KEVDLT 490

Query: 493 PGIGLTMPKAIPLEAMCKPRASITHLLSQL 523
           PGIGLTMPKA PL AM KPRA I HLLSQL
Sbjct: 491 PGIGLTMPKARPLVAMFKPRAPIIHLLSQL 511

BLAST of Cmc07g0183611 vs. NCBI nr
Match: XP_023523499.1 (cytochrome P450 81E8-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 791.6 bits (2043), Expect = 4.2e-225
Identity = 398/510 (78.04%), Postives = 440/510 (86.27%), Query Frame = 0

Query: 13  FSHFNFPLLAIFLTTLFLISKFRRSEHTKLLLPPSPPSLPVIGHLHLLKEPFHQMLQDLS 72
           FS+F  PLL + L  L LI KFR+++H    LPP PPSLPVIGHLHLLKEPFHQMLQDLS
Sbjct: 11  FSNFEVPLLVLIL-ALLLILKFRKADHKN--LPPGPPSLPVIGHLHLLKEPFHQMLQDLS 70

Query: 73  LKYGPVFSLTLGFRPLVVVSSPSAVHECFTKNDIVFASRPRMLSGKILNYDYTAVGATAY 132
            KYGP+ SL+LGFRP+VVVSSP+A  ECF+KNDI+FASRPRM+SGKILNYDYTAVGATAY
Sbjct: 71  HKYGPILSLSLGFRPVVVVSSPAAAQECFSKNDIIFASRPRMISGKILNYDYTAVGATAY 130

Query: 133 GQHWRIMRRIATTELLSTKRLNYFSKLREEEVKLWVKRLYGSVTIREVQKGGRVGLRSKL 192
           GQHWRIMRRIATTELLST RLN    +R++EVKLW+K LY       V    RV +RSKL
Sbjct: 131 GQHWRIMRRIATTELLSTYRLNALINIRQQEVKLWLKSLYRISGKNNV----RVEIRSKL 190

Query: 193 TELSFNIALMLMAGRRYFGEEVEEGESEEAKELKETMKQVPLLSGASYPADFLSVLKLID 252
           TELSFNIALMLM G+RYFG+E  + +SEEA+ELK TMKQVPLLSGASYPADFLSVLKL+D
Sbjct: 191 TELSFNIALMLMVGKRYFGDEAVD-DSEEARELKNTMKQVPLLSGASYPADFLSVLKLVD 250

Query: 253 FQGFRKRLAKVRVDGDAFAQSLVDERRELKGSWCERKTMIDAMLSLQESDPEYYKDDIIK 312
           +QGFRKRLAKVRVDGDAFAQSL+DERR  K    ERKTMID++LS+Q+ +PEYYKDDIIK
Sbjct: 251 YQGFRKRLAKVRVDGDAFAQSLIDERRNKKADDNERKTMIDSLLSMQQMEPEYYKDDIIK 310

Query: 313 GQFLTLLAAGTDTIASTLEWAMSLLLNHPIVMNKAWTEINQVVGDQRLVREADTNNLHYL 372
           GQFLTLLAAGTDT A TLEWA+SLLLNHP  M KAWTEIN+ VGD+RLV E D N LHYL
Sbjct: 311 GQFLTLLAAGTDTTAGTLEWALSLLLNHPTAMKKAWTEINEHVGDERLVEEMDLNQLHYL 370

Query: 373 QSIINETYRLFPALPILVPRESSEDCTIGGFDIPKGTMLVVNAWAVHRNPEVWEDPNCFK 432
           Q IINETYRLFPALPILVPRESSEDCTIGGFD+PKGTMLVVNAWA+HR+P+VWEDPN F+
Sbjct: 371 QGIINETYRLFPALPILVPRESSEDCTIGGFDVPKGTMLVVNAWAIHRDPKVWEDPNSFR 430

Query: 433 PERFKQGETVEVSKLLPFGMGRRACPGAGLAHRVVSLALATLIQCFEWGKLDDKKDVDLC 492
           PERFK+GE+VEVSKLLPFGMGRRACPGAGLAHRVV LALATLIQCFEW +L D K+VDL 
Sbjct: 431 PERFKEGESVEVSKLLPFGMGRRACPGAGLAHRVVGLALATLIQCFEWERL-DSKEVDLT 490

Query: 493 PGIGLTMPKAIPLEAMCKPRASITHLLSQL 523
           PGIGLTMPKA PL AM KPR  I HLLSQL
Sbjct: 491 PGIGLTMPKASPLVAMFKPRTPIIHLLSQL 511

BLAST of Cmc07g0183611 vs. ExPASy Swiss-Prot
Match: W8JMU7 (Cytochrome P450 81Q32 OS=Catharanthus roseus OX=4058 GN=CYP81Q32 PE=2 SV=1)

HSP 1 Score: 505.4 bits (1300), Expect = 7.9e-142
Identity = 272/520 (52.31%), Postives = 359/520 (69.04%), Query Frame = 0

Query: 3   MEQQTSFFFFFSHFNFPLLAIFLTTLFLISKFRRSEHTKLLLPPSPP-SLPVIGHLHLLK 62
           ME  T  + F +     LL+I L  LF +S+ RR+      LPPSP  +LPVIGHLHL+ 
Sbjct: 1   MESSTLLYTFLA---VVLLSISL-KLFPVSRRRRN------LPPSPGLALPVIGHLHLIG 60

Query: 63  EPFHQMLQDLSLKYGPVFSLTLGFRPLVVVSSPSAVHECFTKNDIVFASRPRMLSGKILN 122
           +  H+ L DLS KYG VFSL LG R ++VVSSP+A  ECFTKNDIVFA+RP  + GK + 
Sbjct: 61  KLLHRSLYDLSKKYGSVFSLQLGNRLVLVVSSPAAAEECFTKNDIVFANRPLFILGKYIG 120

Query: 123 YDYTAVGATAYGQHWRIMRRIATTELLSTKRLNYFSKLREEEVKLWVKRLYGSVTIREVQ 182
           Y+YT +  + YG+HWR +RR+A  E+ S   LN F  +RE+EVK  +  LY S      Q
Sbjct: 121 YNYTTMVGSPYGEHWRNLRRLAAVEIFSAGSLNRFLSIREDEVKQLLLSLYQS----SGQ 180

Query: 183 KGGRVGLRSKLTELSFNIALMLMAGRRYFGEEVEEGESEEAKELKETMKQVPLLSGASYP 242
             G+V ++SKL+ELSFN+ + ++AG+RYFG++V   +S+EAK  +  + +V   +GAS P
Sbjct: 181 DFGKVEMKSKLSELSFNVTMRMVAGKRYFGQDV---DSDEAKLFRALIGEVFEHAGASNP 240

Query: 243 ADFLSVLKLIDFQGFRKRLAKVRVDGDAFAQSLVDERRELKGSWCERKTMIDAMLSLQES 302
            DF+  L+ IDF+ + K+++K+  + DAF Q L+ E R  K +     TMID +LSLQES
Sbjct: 241 GDFVPFLRWIDFKNYEKKVSKISQEMDAFLQRLIHESRINKNN----VTMIDHLLSLQES 300

Query: 303 DPEYYKDDIIKGQFLTLLAAGTDTIASTLEWAMSLLLNHPIVMNKAWTEINQVVGDQRLV 362
            PEYY D IIKG  + LL AGTDT A T+EWAMSLLLNHP  + KA TEI   VG  RL+
Sbjct: 301 QPEYYTDQIIKGIIMVLLLAGTDTSAVTVEWAMSLLLNHPETLEKARTEIETQVGSNRLI 360

Query: 363 READTNNLHYLQSIINETYRLFPALPILVPRESSEDCTIGGFDIPKGTMLVVNAWAVHRN 422
            E D   L YL +II+ET+RL PA P+LVP ESS+DC + G+D+PKGT+L+VNAWA+HR+
Sbjct: 361 EEQDLPKLTYLHNIISETFRLCPAAPMLVPHESSDDCKVQGYDVPKGTILLVNAWAIHRD 420

Query: 423 PEVWEDPNCFKPERFKQGETVEVSKLLPFGMGRRACPGAGLAHRVVSLALATLIQCFEWG 482
           PE W++P  FKPER   G  +E SKL+PFGMGRR+CPG+GLA RVV L L  LIQCFEW 
Sbjct: 421 PEFWDEPTLFKPER-HGGVELEPSKLMPFGMGRRSCPGSGLAQRVVGLTLGALIQCFEWK 480

Query: 483 KLDDKKDVDLCPGIGLTMPKAIPLEAMCKPRASITHLLSQ 522
           ++ + K +D+  G GLTMPKA PLEA+CKPR  +  ++S+
Sbjct: 481 RIGEAK-IDMAEGSGLTMPKAQPLEALCKPRNILHKVVSE 497

BLAST of Cmc07g0183611 vs. ExPASy Swiss-Prot
Match: P93147 (Isoflavone 2'-hydroxylase OS=Glycyrrhiza echinata OX=46348 GN=CYP81E1 PE=1 SV=2)

HSP 1 Score: 453.8 bits (1166), Expect = 2.7e-126
Identity = 231/503 (45.92%), Postives = 329/503 (65.41%), Query Frame = 0

Query: 22  AIFLTTLFLISKFRRSEHTKLLLPPSPPSLPVIGHLHLLKEPFHQMLQDLSLKYGPVFSL 81
           ++F   LF I            LPP PPSLP+IG+LH LK P H+  + LS KYG VFSL
Sbjct: 10  SVFYLALFFIFNIVIRARKFKNLPPGPPSLPIIGNLHHLKRPLHRTFKGLSEKYGHVFSL 69

Query: 82  TLGFRPLVVVSSPSAVHECFTKNDIVFASRPRMLSGKILNYDYTAVGATAYGQHWRIMRR 141
             G R +VVVSS S   +CFTKND+V A+RPR LSGK + Y+YT +G+T+YG+HWR +RR
Sbjct: 70  WFGSRLVVVVSSASEFQQCFTKNDVVLANRPRFLSGKYIFYNYTTLGSTSYGEHWRNLRR 129

Query: 142 IATTELLSTKRLNYFSKLREEEVKLWVKRLY--GSVTIREVQKGGRVGLRSKLTELSFNI 201
           I   ++LS  R+N FS +R +E +  + RL    S    E++      L S+L +++FN 
Sbjct: 130 ITALDVLSNHRINSFSGIRRDETQRLITRLADDSSTNFAEME------LSSRLYDMTFNN 189

Query: 202 ALMLMAGRRYFGEEVEEGESEEAKELKETMKQVPLLSGASYPADFLSVLKLIDFQGFRKR 261
            + +++G+RY+GE+ +  + +EA + ++ + ++  LSGA+   DF+ +L+ +DF+   KR
Sbjct: 190 IMRMISGKRYYGEDCDTSDLQEASQFRDMVSELLQLSGANNKTDFMPLLRFLDFENLEKR 249

Query: 262 LAKVRVDGDAFAQSLVDERRELKGSWCERKTMIDAMLSLQESDPEYYKDDIIKGQFLTLL 321
           L  +    DAF + L++E R  K       TMID +L+LQ+S PEYY D IIKG  L +L
Sbjct: 250 LKDISGKTDAFLRGLIEEHRTKKE---RANTMIDHLLNLQDSQPEYYTDQIIKGLALAML 309

Query: 322 AAGTDTIASTLEWAMSLLLNHPIVMNKAWTEINQVVGDQRLVREADTNNLHYLQSIINET 381
            AGTD+ A TLEW+MS LLNHP V+ K   E++  VG  RLV E+D   L YL+++INET
Sbjct: 310 LAGTDSSAVTLEWSMSNLLNHPEVLKKVKDELDTHVGQDRLVDESDLPKLTYLKNVINET 369

Query: 382 YRLFPALPILVPRESSEDCTIGGFDIPKGTMLVVNAWAVHRNPEVWEDPNCFKPERF-KQ 441
            RL+   P+L+P  +S++C IGG+ +P+ T++++NAWA+HR+PE+W +   FKPERF K+
Sbjct: 370 LRLYTPAPLLLPHSTSDECNIGGYKVPQDTIVLINAWAIHRDPELWTEATTFKPERFEKK 429

Query: 442 GETVEVSKLLPFGMGRRACPGAGLAHRVVSLALATLIQCFEWGKLDDKKDVDLCPGIGLT 501
           G   E+ KL+ FGMGRRACPG GLA R +S+ LA LIQCF+W KL +   +DL    G T
Sbjct: 430 G---ELEKLIAFGMGRRACPGEGLAIRAISMTLALLIQCFDW-KLINGDKIDLAERDGFT 489

Query: 502 MPKAIPLEAMCKPRASITHLLSQ 522
           + K +PL+AMCK R  I  +  Q
Sbjct: 490 LTKLVPLKAMCKSRPVINKVFKQ 499

BLAST of Cmc07g0183611 vs. ExPASy Swiss-Prot
Match: Q6WNQ9 (Isoflavone 3'-hydroxylase (Fragment) OS=Medicago truncatula OX=3880 GN=CYP81E9 PE=1 SV=1)

HSP 1 Score: 450.3 bits (1157), Expect = 3.0e-125
Identity = 229/498 (45.98%), Postives = 328/498 (65.86%), Query Frame = 0

Query: 16  FNFPLLAI-FLTTLFLISKF--RRSEHTKLLLPPSPPSLPVIGHLHLLKEPFHQMLQDLS 75
           F + LL++ F+ T+ ++ K   RR ++    LPP PP++P+IG+LH LK P H+    LS
Sbjct: 3   FYYSLLSLSFIITIKILLKITSRRLKN----LPPGPPTIPIIGNLHHLKHPLHRTFTTLS 62

Query: 76  LKYGPVFSLTLGFRPLVVVSSPSAVHECFTKNDIVFASRPRMLSGKILNYDYTAVGATAY 135
             YG +FSL  G R +VVVSSPS  HECFTKNDI+ A+RPR L+GK + Y+YT +G+ +Y
Sbjct: 63  QTYGDIFSLWFGSRLVVVVSSPSLAHECFTKNDIILANRPRFLTGKYIFYNYTTLGSASY 122

Query: 136 GQHWRIMRRIATTELLSTKRLNYFSKLREEEVKLWVKRLYGSVTIREVQKGGRVGLRSKL 195
           G HWR +RRI T ++LS  RLN F  +R +E    +++L   V + E     +V LR +L
Sbjct: 123 GDHWRNLRRITTIDVLSNNRLNSFLGVRRDETNRLIQKLLKDV-VSEGFGFTKVELRPRL 182

Query: 196 TELSFNIALMLMAGRRYFGEEVEEGESEEAKELKETMKQVPLLSGASYPADFLSVLKLID 255
           TE++FN  + +++G+RY+G++ +  + EEAK+ +E + ++  L GA+   DFL +L+++D
Sbjct: 183 TEMTFNAMMRMISGKRYYGDDGDVSDVEEAKQFREIISEMMSLLGANNKGDFLPLLRVVD 242

Query: 256 FQGFRKRLAKVRVDGDAFAQSLVDERRELKGSWCERKTMIDAMLSLQESDPEYYKDDIIK 315
                KR  ++    +AF + L++E R       +  TMID +L L ES PEYY D +IK
Sbjct: 243 LDNLEKRCKRIAKRSNAFLEGLIEEHRR-GNIHSDGGTMIDHLLKLSESQPEYYSDHLIK 302

Query: 316 GQFLTLLAAGTDTIASTLEWAMSLLLNHPIVMNKAWTEINQVVGDQRLVREADTNNLHYL 375
           G    +L AGTDT A T+EW MS LLNHP V+ KA  E++  +G  +LV E D + L YL
Sbjct: 303 GLIQGMLLAGTDTSAVTIEWVMSELLNHPEVLKKAKEELDTQIGKNKLVDEQDLSKLPYL 362

Query: 376 QSIINETYRLFPALPILVPRESSEDCTIGGFDIPKGTMLVVNAWAVHRNPEVWEDPNCFK 435
           Q+II+ET RL P  P+L+P  SSEDCTIG F++PK T+++ N W +HR+P+ W D   FK
Sbjct: 363 QNIISETLRLHPPAPLLLPHYSSEDCTIGEFNVPKDTIILTNVWGIHRDPKHWNDALSFK 422

Query: 436 PERFKQGETVEVSKLLPFGMGRRACPGAGLAHRVVSLALATLIQCFEWGKLDDKKDVDLC 495
           PERF++ E  EV+K++ FG+GRRACPG  LA R V   +  LIQCFEW +  ++K +D+ 
Sbjct: 423 PERFEKEE--EVNKVMAFGLGRRACPGLSLAQRTVGFTVGLLIQCFEWERESEEK-LDMM 482

Query: 496 PGIGLTMPKAIPLEAMCK 511
            G G+TMP  IPL AMCK
Sbjct: 483 EGKGITMPMKIPLRAMCK 491

BLAST of Cmc07g0183611 vs. ExPASy Swiss-Prot
Match: Q6WNQ8 (Cytochrome P450 81E8 OS=Medicago truncatula OX=3880 GN=CYP81E8 PE=2 SV=1)

HSP 1 Score: 439.1 bits (1128), Expect = 6.9e-122
Identity = 231/494 (46.76%), Postives = 313/494 (63.36%), Query Frame = 0

Query: 20  LLAIFLTTLFLISKFRRSEHTKLLLPPSPPSLPVIGHLHLLKEPFHQMLQDLSLKYGPVF 79
           ++++F   + L   F  S   K  LPP P  LP+IG+LH LK+P H     LS KYG +F
Sbjct: 9   IISLFFLIITLKVFFNTSRKFK-NLPPGPQCLPIIGNLHQLKQPLHHTFHTLSQKYGQIF 68

Query: 80  SLTLGFRPLVVVSSPSAVHECFTKNDIVFASRPRMLSGKILNYDYTAVGATAYGQHWRIM 139
           SL  G R +VVVSS +   ECFTKNDIV A+RP  L+GK + Y+ T V  + YG HWR +
Sbjct: 69  SLWFGSRLVVVVSSLTIAQECFTKNDIVLANRPHFLTGKYIGYNNTTVAQSPYGDHWRNL 128

Query: 140 RRIATTELLSTKRLNYFSKLREEEVKLWVKRLYGSVTIREVQKGGRVGLRSKLTELSFNI 199
           RRI + E+LS+ RLN F ++R +E    + RL   +  +       V LR   +E++FN 
Sbjct: 129 RRILSIEILSSHRLNSFLEIRRDE----IMRLIQKLAQKSYNGFTEVELRPMFSEMTFNT 188

Query: 200 ALMLMAGRRYFGEEVEEGESEEAKELKETMKQVPLLSGASYPADFLSVLKLIDFQGFRKR 259
            + +++G+RY+G + +  + EEA+  +  +K+V  L GA+   DFL  L+  DF G  KR
Sbjct: 189 IMRMVSGKRYYGNDCDVSDVEEARLFRGIIKEVVSLGGANNVGDFLGFLRWFDFDGLEKR 248

Query: 260 LAKVRVDGDAFAQSLVDERRELKGSWCERKTMIDAMLSLQESDPEYYKDDIIKGQFLTLL 319
           L K+    DAF Q L+DE R  K +     TMID +L+ Q+S PEYY D IIKG  + +L
Sbjct: 249 LKKISKRTDAFLQGLIDEHRFGKRN---SNTMIDHLLTQQQSQPEYYTDQIIKGLMVVML 308

Query: 320 AAGTDTIASTLEWAMSLLLNHPIVMNKAWTEINQVVGDQRLVREADTNNLHYLQSIINET 379
            AGTDT + T+EWAMS LLNHP +M KA  E++  +G  R V E D + L YLQSI+ ET
Sbjct: 309 LAGTDTSSVTIEWAMSNLLNHPEIMKKAKNELDTHIGHDRQVDEHDISKLPYLQSIVYET 368

Query: 380 YRLFPALPILVPRESSEDCTIGGFDIPKGTMLVVNAWAVHRNPEVWEDPNCFKPERF-KQ 439
            RL  A P+LVP  SSED ++GG++IP+ T+L+VNAW +HR+P +W DP CFKPERF K+
Sbjct: 369 LRLHAAAPLLVPHLSSEDFSLGGYNIPQNTILMVNAWVIHRDPNLWSDPTCFKPERFEKE 428

Query: 440 GETVEVSKLLPFGMGRRACPGAGLAHRVVSLALATLIQCFEWGKLDDKKDVDLCPGIGLT 499
           G   EV+KLL FG+GRRACPG  L+ R   L L  LIQCFEW ++ ++K +D+    G+T
Sbjct: 429 G---EVNKLLSFGLGRRACPGENLSQRTEGLTLGLLIQCFEWKRIGEEK-IDMVEAKGIT 488

Query: 500 MPKAIPLEAMCKPR 513
             K   L AMCK R
Sbjct: 489 AGKKTSLNAMCKVR 490

BLAST of Cmc07g0183611 vs. ExPASy Swiss-Prot
Match: Q6WNR0 (Isoflavone 2'-hydroxylase OS=Medicago truncatula OX=3880 GN=CYP81E7 PE=1 SV=1)

HSP 1 Score: 435.3 bits (1118), Expect = 1.0e-120
Identity = 214/492 (43.50%), Postives = 324/492 (65.85%), Query Frame = 0

Query: 22  AIFLTTLFLISKFRRSEHTKLLLPPSPPSLPVIGHLHLLKEPFHQMLQDLSLKYGPVFSL 81
           ++F  ++F I +          LPP P SLP+IG+LH LK P ++  + L+ KYG V SL
Sbjct: 10  SLFYLSIFFIIRLLFQSRKFKNLPPGPTSLPIIGNLHHLKRPLNRTFKALTEKYGNVISL 69

Query: 82  TLGFRPLVVVSSPSAVHECFTKNDIVFASRPRMLSGKILNYDYTAVGATAYGQHWRIMRR 141
             G R +VVVSS S   ECFTKND+V A+RPR LSGK + Y+YT +G+T+YG+HWR +RR
Sbjct: 70  WFGSRLVVVVSSLSEFQECFTKNDVVLANRPRFLSGKYIFYNYTTLGSTSYGEHWRNLRR 129

Query: 142 IATTELLSTKRLNYFSKLREEEVKLWVKRLYGSVTIREVQKGGRVGLRSKLTELSFNIAL 201
           I + ++LS  R+N F+ +R +E +  +K+L    +     K   V L  +  +++FN  +
Sbjct: 130 ITSLDVLSNHRINNFAPIRRDETQRLIKKLAEDSS----TKFAEVELTFRFFDMTFNNIM 189

Query: 202 MLMAGRRYFGEEVEEGESEEAKELKETMKQVPLLSGASYPADFLSVLKLIDFQGFRKRLA 261
            +++G+RY+G++ +  E +EA + ++ + ++  LSGA+   DF+ +LK +DF+   KR+ 
Sbjct: 190 RMISGKRYYGDDCDISEVQEASQFRDMVSELLQLSGANNKTDFMPLLKFLDFENLEKRVK 249

Query: 262 KVRVDGDAFAQSLVDERRELKGSWCERKTMIDAMLSLQESDPEYYKDDIIKGQFLTLLAA 321
           ++    D F   L+ E+R  K       TMID +L++QES PEYY D IIKG  L +L A
Sbjct: 250 RIGEKNDVFLSGLLQEQRSKKE---RTNTMIDHLLNMQESQPEYYTDTIIKGLCLAMLLA 309

Query: 322 GTDTIASTLEWAMSLLLNHPIVMNKAWTEINQVVGDQRLVREADTNNLHYLQSIINETYR 381
           GTD+ A TLEW MS +LN+P V+ K   E++  VG  RLV E+D   L YL+++I ET R
Sbjct: 310 GTDSSAVTLEWTMSNILNYPEVLKKVRDEVDTHVGQDRLVDESDLPKLTYLRNVIYETLR 369

Query: 382 LFPALPILVPRESSEDCTIGGFDIPKGTMLVVNAWAVHRNPEVWEDPNCFKPERF-KQGE 441
           L+   P+L+P  ++++C +GG+ +P+ T++++NAWA+HR+PE W +   FKPERF K+G 
Sbjct: 370 LYTPAPLLLPHSTADECIMGGYKVPRDTIVLINAWAIHRDPETWSEATTFKPERFDKKG- 429

Query: 442 TVEVSKLLPFGMGRRACPGAGLAHRVVSLALATLIQCFEWGKLDDKKDVDLCPGIGLTMP 501
             E+ K++ FGMGRRACPG GLA R +S+ LA L+QCF+W +++D+K +D+    G TM 
Sbjct: 430 --ELEKMIAFGMGRRACPGEGLALRAISMTLALLVQCFDWKRINDEK-IDMSERDGFTMT 489

Query: 502 KAIPLEAMCKPR 513
           K +PL+AMCK R
Sbjct: 490 KLLPLKAMCKTR 490

BLAST of Cmc07g0183611 vs. ExPASy TrEMBL
Match: A0A5D3D9B5 (Isoflavone 2'-hydroxylase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold134G002940 PE=3 SV=1)

HSP 1 Score: 1039.6 bits (2687), Expect = 4.3e-300
Identity = 518/522 (99.23%), Postives = 520/522 (99.62%), Query Frame = 0

Query: 1   MDMEQQTSFFFFFSHFNFPLLAIFLTTLFLISKFRRSEHTKLLLPPSPPSLPVIGHLHLL 60
           MDMEQQTSFFFFFSHFNFPLLAIFLTTLFLISKFRRSEHTKLLLPPSPPSLPVIGHLHLL
Sbjct: 1   MDMEQQTSFFFFFSHFNFPLLAIFLTTLFLISKFRRSEHTKLLLPPSPPSLPVIGHLHLL 60

Query: 61  KEPFHQMLQDLSLKYGPVFSLTLGFRPLVVVSSPSAVHECFTKNDIVFASRPRMLSGKIL 120
           KEPFHQMLQDLSLKYGPVFSLTLGFRPLVVVSSPSAVHECFTKNDIVFASRPRMLSGKIL
Sbjct: 61  KEPFHQMLQDLSLKYGPVFSLTLGFRPLVVVSSPSAVHECFTKNDIVFASRPRMLSGKIL 120

Query: 121 NYDYTAVGATAYGQHWRIMRRIATTELLSTKRLNYFSKLREEEVKLWVKRLYGSVTIREV 180
           NYDYTAVGATAYGQHWRIMRRIATTELLSTKRLN FSKLREEEVKLWVKRLYGSVTIREV
Sbjct: 121 NYDYTAVGATAYGQHWRIMRRIATTELLSTKRLNDFSKLREEEVKLWVKRLYGSVTIREV 180

Query: 181 QKGGRVGLRSKLTELSFNIALMLMAGRRYFGEEVEEGESEEAKELKETMKQVPLLSGASY 240
           QKGGRVGLRSKLTELSFNIALML AGRRYFGEEVEEGESEEAKELKETMKQVPLLSGASY
Sbjct: 181 QKGGRVGLRSKLTELSFNIALMLTAGRRYFGEEVEEGESEEAKELKETMKQVPLLSGASY 240

Query: 241 PADFLSVLKLIDFQGFRKRLAKVRVDGDAFAQSLVDERRELKGSWCERKTMIDAMLSLQE 300
           PADFLSVLKLIDFQGFRKRLAKVRVDGDAFAQSLVDERRELKGSWCERKTMIDAMLSLQE
Sbjct: 241 PADFLSVLKLIDFQGFRKRLAKVRVDGDAFAQSLVDERRELKGSWCERKTMIDAMLSLQE 300

Query: 301 SDPEYYKDDIIKGQFLTLLAAGTDTIASTLEWAMSLLLNHPIVMNKAWTEINQVVGDQRL 360
           SDPEYYKDDIIKGQFLTLLAAGTDTIASTLEWAMSLLLNHPIVMNKAWTEINQVVGDQRL
Sbjct: 301 SDPEYYKDDIIKGQFLTLLAAGTDTIASTLEWAMSLLLNHPIVMNKAWTEINQVVGDQRL 360

Query: 361 VREADTNNLHYLQSIINETYRLFPALPILVPRESSEDCTIGGFDIPKGTMLVVNAWAVHR 420
           VREADTNNLHYLQSIINETYRLFPALPILVPRESSEDCTIGGFDIPKGTMLVVNAWAVHR
Sbjct: 361 VREADTNNLHYLQSIINETYRLFPALPILVPRESSEDCTIGGFDIPKGTMLVVNAWAVHR 420

Query: 421 NPEVWEDPNCFKPERFKQGETVEVSKLLPFGMGRRACPGAGLAHRVVSLALATLIQCFEW 480
           NPEVWEDPNCFKPERFKQGETVEVSKLLPFGMGRRACPGAGLAHRVVSLALATLIQCFEW
Sbjct: 421 NPEVWEDPNCFKPERFKQGETVEVSKLLPFGMGRRACPGAGLAHRVVSLALATLIQCFEW 480

Query: 481 GKLDDKKDVDLCPGIGLTMPKAIPLEAMCKPRASITHLLSQL 523
           GKLD+KKDVDLCPGIGLTMPKAIPLEAMCKPRAS+THLLSQL
Sbjct: 481 GKLDNKKDVDLCPGIGLTMPKAIPLEAMCKPRASVTHLLSQL 522

BLAST of Cmc07g0183611 vs. ExPASy TrEMBL
Match: A0A1S3BTN1 (isoflavone 2'-hydroxylase-like OS=Cucumis melo OX=3656 GN=LOC103493583 PE=3 SV=1)

HSP 1 Score: 1039.6 bits (2687), Expect = 4.3e-300
Identity = 518/522 (99.23%), Postives = 520/522 (99.62%), Query Frame = 0

Query: 1   MDMEQQTSFFFFFSHFNFPLLAIFLTTLFLISKFRRSEHTKLLLPPSPPSLPVIGHLHLL 60
           MDMEQQTSFFFFFSHFNFPLLAIFLTTLFLISKFRRSEHTKLLLPPSPPSLPVIGHLHLL
Sbjct: 1   MDMEQQTSFFFFFSHFNFPLLAIFLTTLFLISKFRRSEHTKLLLPPSPPSLPVIGHLHLL 60

Query: 61  KEPFHQMLQDLSLKYGPVFSLTLGFRPLVVVSSPSAVHECFTKNDIVFASRPRMLSGKIL 120
           KEPFHQMLQDLSLKYGPVFSLTLGFRPLVVVSSPSAVHECFTKNDIVFASRPRMLSGKIL
Sbjct: 61  KEPFHQMLQDLSLKYGPVFSLTLGFRPLVVVSSPSAVHECFTKNDIVFASRPRMLSGKIL 120

Query: 121 NYDYTAVGATAYGQHWRIMRRIATTELLSTKRLNYFSKLREEEVKLWVKRLYGSVTIREV 180
           NYDYTAVGATAYGQHWRIMRRIATTELLSTKRLN FSKLREEEVKLWVKRLYGSVTIREV
Sbjct: 121 NYDYTAVGATAYGQHWRIMRRIATTELLSTKRLNDFSKLREEEVKLWVKRLYGSVTIREV 180

Query: 181 QKGGRVGLRSKLTELSFNIALMLMAGRRYFGEEVEEGESEEAKELKETMKQVPLLSGASY 240
           QKGGRVGLRSKLTELSFNIALML AGRRYFGEEVEEGESEEAKELKETMKQVPLLSGASY
Sbjct: 181 QKGGRVGLRSKLTELSFNIALMLTAGRRYFGEEVEEGESEEAKELKETMKQVPLLSGASY 240

Query: 241 PADFLSVLKLIDFQGFRKRLAKVRVDGDAFAQSLVDERRELKGSWCERKTMIDAMLSLQE 300
           PADFLSVLKLIDFQGFRKRLAKVRVDGDAFAQSLVDERRELKGSWCERKTMIDAMLSLQE
Sbjct: 241 PADFLSVLKLIDFQGFRKRLAKVRVDGDAFAQSLVDERRELKGSWCERKTMIDAMLSLQE 300

Query: 301 SDPEYYKDDIIKGQFLTLLAAGTDTIASTLEWAMSLLLNHPIVMNKAWTEINQVVGDQRL 360
           SDPEYYKDDIIKGQFLTLLAAGTDTIASTLEWAMSLLLNHPIVMNKAWTEINQVVGDQRL
Sbjct: 301 SDPEYYKDDIIKGQFLTLLAAGTDTIASTLEWAMSLLLNHPIVMNKAWTEINQVVGDQRL 360

Query: 361 VREADTNNLHYLQSIINETYRLFPALPILVPRESSEDCTIGGFDIPKGTMLVVNAWAVHR 420
           VREADTNNLHYLQSIINETYRLFPALPILVPRESSEDCTIGGFDIPKGTMLVVNAWAVHR
Sbjct: 361 VREADTNNLHYLQSIINETYRLFPALPILVPRESSEDCTIGGFDIPKGTMLVVNAWAVHR 420

Query: 421 NPEVWEDPNCFKPERFKQGETVEVSKLLPFGMGRRACPGAGLAHRVVSLALATLIQCFEW 480
           NPEVWEDPNCFKPERFKQGETVEVSKLLPFGMGRRACPGAGLAHRVVSLALATLIQCFEW
Sbjct: 421 NPEVWEDPNCFKPERFKQGETVEVSKLLPFGMGRRACPGAGLAHRVVSLALATLIQCFEW 480

Query: 481 GKLDDKKDVDLCPGIGLTMPKAIPLEAMCKPRASITHLLSQL 523
           GKLD+KKDVDLCPGIGLTMPKAIPLEAMCKPRAS+THLLSQL
Sbjct: 481 GKLDNKKDVDLCPGIGLTMPKAIPLEAMCKPRASVTHLLSQL 522

BLAST of Cmc07g0183611 vs. ExPASy TrEMBL
Match: A0A0A0L2I1 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G641760 PE=3 SV=1)

HSP 1 Score: 963.8 bits (2490), Expect = 3.0e-277
Identity = 482/524 (91.98%), Postives = 499/524 (95.23%), Query Frame = 0

Query: 3   MEQQTSFFFFFSH----FNFPLLAIFLTTLFLISKFRRSEHTKLLLPPSPPSLPVIGHLH 62
           MEQQTSFFF   H     + PLLAIFLT+LFLISKFRR++HTK LLPPSPPSLPVIGHLH
Sbjct: 1   MEQQTSFFFSQDHHFHPISIPLLAIFLTSLFLISKFRRTQHTK-LLPPSPPSLPVIGHLH 60

Query: 63  LLKEPFHQMLQDLSLKYGPVFSLTLGFRPLVVVSSPSAVHECFTKNDIVFASRPRMLSGK 122
           LLKEPFHQMLQD S KYGPVFSLTLGFRPLVVVSSPSAV ECFTKNDIVFASRPRMLSGK
Sbjct: 61  LLKEPFHQMLQDFSKKYGPVFSLTLGFRPLVVVSSPSAVQECFTKNDIVFASRPRMLSGK 120

Query: 123 ILNYDYTAVGATAYGQHWRIMRRIATTELLSTKRLNYFSKLREEEVKLWVKRLYGSVTIR 182
           ILNYDYTAVGATAYGQHWRIMRRIATTELLSTKRLN  SK+REEEVKLWVKRLYGSVT  
Sbjct: 121 ILNYDYTAVGATAYGQHWRIMRRIATTELLSTKRLNDLSKIREEEVKLWVKRLYGSVTRA 180

Query: 183 EVQKGGRVGLRSKLTELSFNIALMLMAGRRYFGEEVEEGESEEAKELKETMKQVPLLSGA 242
           EV KGGRVGLRSKLTELSFNIALMLMAGRRYFG+E+E GESEEAKELKETMKQVPLLSGA
Sbjct: 181 EVNKGGRVGLRSKLTELSFNIALMLMAGRRYFGDEMEAGESEEAKELKETMKQVPLLSGA 240

Query: 243 SYPADFLSVLKLIDFQGFRKRLAKVRVDGDAFAQSLVDERRELKGSWCERKTMIDAMLSL 302
           SYPADFLSVLKLIDFQGFRKRLAKVRVDGDAFAQSLVDERR+LKGSWCERKTMID+ML L
Sbjct: 241 SYPADFLSVLKLIDFQGFRKRLAKVRVDGDAFAQSLVDERRKLKGSWCERKTMIDSMLCL 300

Query: 303 QESDPEYYKDDIIKGQFLTLLAAGTDTIASTLEWAMSLLLNHPIVMNKAWTEINQVVGDQ 362
           QESDPEYYKDDIIKGQFLTLLAAGTDT ASTLEWAMSLLLNHP V+ KAWTEINQVVGDQ
Sbjct: 301 QESDPEYYKDDIIKGQFLTLLAAGTDTTASTLEWAMSLLLNHPTVLEKAWTEINQVVGDQ 360

Query: 363 RLVREADTNNLHYLQSIINETYRLFPALPILVPRESSEDCTIGGFDIPKGTMLVVNAWAV 422
           RL+READTN LHYLQ+IINETYRLFPALPILVPRESSEDCTIGGFDIPKGTMLVVNAWAV
Sbjct: 361 RLIREADTNKLHYLQAIINETYRLFPALPILVPRESSEDCTIGGFDIPKGTMLVVNAWAV 420

Query: 423 HRNPEVWEDPNCFKPERFKQGETVEVSKLLPFGMGRRACPGAGLAHRVVSLALATLIQCF 482
           HR+P VWEDPNCFKPERFKQGETVEV+KLLPFGMGRRACPGAGLAHRVVSLALATLIQCF
Sbjct: 421 HRDPNVWEDPNCFKPERFKQGETVEVNKLLPFGMGRRACPGAGLAHRVVSLALATLIQCF 480

Query: 483 EWGKLDDKKDVDLCPGIGLTMPKAIPLEAMCKPRASITHLLSQL 523
           EWGKLD++KDVDLCPGIGLTMPKAIPLEAMCKPRAS+THLLSQL
Sbjct: 481 EWGKLDNQKDVDLCPGIGLTMPKAIPLEAMCKPRASVTHLLSQL 523

BLAST of Cmc07g0183611 vs. ExPASy TrEMBL
Match: A0A6J1FJ78 (cytochrome P450 81E8-like OS=Cucurbita moschata OX=3662 GN=LOC111445861 PE=3 SV=1)

HSP 1 Score: 794.7 bits (2051), Expect = 2.4e-226
Identity = 400/510 (78.43%), Postives = 442/510 (86.67%), Query Frame = 0

Query: 13  FSHFNFPLLAIFLTTLFLISKFRRSEHTKLLLPPSPPSLPVIGHLHLLKEPFHQMLQDLS 72
           FS+F  PLL + L  L LI KFR++EH    LPP PPSLPVIGHLHLLKEPFHQMLQDLS
Sbjct: 11  FSNFEVPLLVLIL-ALLLIFKFRKAEHKN--LPPGPPSLPVIGHLHLLKEPFHQMLQDLS 70

Query: 73  LKYGPVFSLTLGFRPLVVVSSPSAVHECFTKNDIVFASRPRMLSGKILNYDYTAVGATAY 132
            KYGP+ SL+LGFRP+VVVSSP+A  ECF+KNDI+FASRPRM+SGKILNYDYTAVGATAY
Sbjct: 71  QKYGPILSLSLGFRPVVVVSSPAAAQECFSKNDIIFASRPRMISGKILNYDYTAVGATAY 130

Query: 133 GQHWRIMRRIATTELLSTKRLNYFSKLREEEVKLWVKRLYGSVTIREVQKGGRVGLRSKL 192
           GQHWRIMRRIATTELLST RLN    +R++EVKLW+K LY       V    RV +RSKL
Sbjct: 131 GQHWRIMRRIATTELLSTYRLNALINIRQQEVKLWLKSLYRISGKNNV----RVEIRSKL 190

Query: 193 TELSFNIALMLMAGRRYFGEEVEEGESEEAKELKETMKQVPLLSGASYPADFLSVLKLID 252
           TELSFNIALMLM G+RYFG+E  + +SEEA+ELK TMKQVPLLSGASYPADFLSVLKL+D
Sbjct: 191 TELSFNIALMLMVGKRYFGDEAVD-DSEEARELKNTMKQVPLLSGASYPADFLSVLKLVD 250

Query: 253 FQGFRKRLAKVRVDGDAFAQSLVDERRELKGSWCERKTMIDAMLSLQESDPEYYKDDIIK 312
           +QGFRKRLAKVRVDGDAFAQSL+DERR  K    ERKTMID++LS+Q+ +PEYYKDDIIK
Sbjct: 251 YQGFRKRLAKVRVDGDAFAQSLIDERRNKKADDNERKTMIDSLLSMQQMEPEYYKDDIIK 310

Query: 313 GQFLTLLAAGTDTIASTLEWAMSLLLNHPIVMNKAWTEINQVVGDQRLVREADTNNLHYL 372
           GQFLTLLAAGTDT A TLEWA+SLLLNHP  M KAWTEIN+ VGD+RLV E D N LHYL
Sbjct: 311 GQFLTLLAAGTDTTAGTLEWALSLLLNHPTAMKKAWTEINEHVGDERLVEEMDLNQLHYL 370

Query: 373 QSIINETYRLFPALPILVPRESSEDCTIGGFDIPKGTMLVVNAWAVHRNPEVWEDPNCFK 432
           Q IINETYRLFPALPILVPRESSEDCTIGGFD+PKGTMLVVNAWA+HR+P+VWEDPN F+
Sbjct: 371 QGIINETYRLFPALPILVPRESSEDCTIGGFDVPKGTMLVVNAWAIHRDPKVWEDPNSFR 430

Query: 433 PERFKQGETVEVSKLLPFGMGRRACPGAGLAHRVVSLALATLIQCFEWGKLDDKKDVDLC 492
           PERFK+GE+VEVSKLLPFGMGRRACPGAGLAHRVV LALATLIQCFEW +LD+ K+VDL 
Sbjct: 431 PERFKEGESVEVSKLLPFGMGRRACPGAGLAHRVVGLALATLIQCFEWERLDN-KEVDLT 490

Query: 493 PGIGLTMPKAIPLEAMCKPRASITHLLSQL 523
           PGIGLTMPKA PL AM KPRA I HLLSQL
Sbjct: 491 PGIGLTMPKARPLVAMFKPRAPIIHLLSQL 511

BLAST of Cmc07g0183611 vs. ExPASy TrEMBL
Match: A0A6J1J4B3 (isoflavone 3'-hydroxylase-like OS=Cucurbita maxima OX=3661 GN=LOC111481120 PE=3 SV=1)

HSP 1 Score: 785.0 bits (2026), Expect = 1.9e-223
Identity = 394/510 (77.25%), Postives = 440/510 (86.27%), Query Frame = 0

Query: 13  FSHFNFPLLAIFLTTLFLISKFRRSEHTKLLLPPSPPSLPVIGHLHLLKEPFHQMLQDLS 72
           FS+F   LL I +  L +I KFR+++H    LPP PPSLPVIGHLHLLKEPFHQMLQDLS
Sbjct: 12  FSNFEV-LLLILVLALLMIFKFRKADHKN--LPPGPPSLPVIGHLHLLKEPFHQMLQDLS 71

Query: 73  LKYGPVFSLTLGFRPLVVVSSPSAVHECFTKNDIVFASRPRMLSGKILNYDYTAVGATAY 132
            KYGP+ SL+LGFRP+VVVSSP+A  ECF+KNDI+FASRPRM+SGKILNYDYTAVGATAY
Sbjct: 72  QKYGPILSLSLGFRPVVVVSSPAAAQECFSKNDIIFASRPRMISGKILNYDYTAVGATAY 131

Query: 133 GQHWRIMRRIATTELLSTKRLNYFSKLREEEVKLWVKRLYGSVTIREVQKGGRVGLRSKL 192
           GQHWRIMRRIATTELLST RLN    +R++EVKLW+K LY       V    RV +RSKL
Sbjct: 132 GQHWRIMRRIATTELLSTHRLNALINIRQQEVKLWLKSLYRITGKNNV----RVEIRSKL 191

Query: 193 TELSFNIALMLMAGRRYFGEEVEEGESEEAKELKETMKQVPLLSGASYPADFLSVLKLID 252
           TELSFNIALMLM G+RYFG+E  + +SEEA+ELK TMKQVPLLSGASYPADFLSVLKL+D
Sbjct: 192 TELSFNIALMLMVGKRYFGDEAVD-DSEEARELKNTMKQVPLLSGASYPADFLSVLKLVD 251

Query: 253 FQGFRKRLAKVRVDGDAFAQSLVDERRELKGSWCERKTMIDAMLSLQESDPEYYKDDIIK 312
           +QGFRK+LAKVRVDGDAFAQSL+DERR  K    ERKTMID++LS+Q+ +PEYYKD IIK
Sbjct: 252 YQGFRKKLAKVRVDGDAFAQSLIDERRNKKADHNERKTMIDSLLSMQQMEPEYYKDHIIK 311

Query: 313 GQFLTLLAAGTDTIASTLEWAMSLLLNHPIVMNKAWTEINQVVGDQRLVREADTNNLHYL 372
           GQFLTLLAAGTDT A TLEWA+SLLLNHP  M KAWTEIN+ VGD+RLV E D N LHYL
Sbjct: 312 GQFLTLLAAGTDTTAGTLEWALSLLLNHPTAMKKAWTEINEHVGDERLVEEMDLNQLHYL 371

Query: 373 QSIINETYRLFPALPILVPRESSEDCTIGGFDIPKGTMLVVNAWAVHRNPEVWEDPNCFK 432
           Q IINETYRLFPALPILVPRESSEDCTIGGFD+P GTMLVVNAWA+HR+P+VWEDPN F+
Sbjct: 372 QGIINETYRLFPALPILVPRESSEDCTIGGFDVPIGTMLVVNAWAIHRDPKVWEDPNSFR 431

Query: 433 PERFKQGETVEVSKLLPFGMGRRACPGAGLAHRVVSLALATLIQCFEWGKLDDKKDVDLC 492
           PERFK+GE+VEVSKLLPFGMGRRACPGAGLAHRVV LALATLIQCFEW +LD+ K+VDL 
Sbjct: 432 PERFKEGESVEVSKLLPFGMGRRACPGAGLAHRVVGLALATLIQCFEWERLDN-KEVDLT 491

Query: 493 PGIGLTMPKAIPLEAMCKPRASITHLLSQL 523
           PGIGLTMPK+ PL AM KPRA ITHLLSQL
Sbjct: 492 PGIGLTMPKSSPLVAMFKPRAPITHLLSQL 512

BLAST of Cmc07g0183611 vs. TAIR 10
Match: AT4G37370.1 (cytochrome P450, family 81, subfamily D, polypeptide 8 )

HSP 1 Score: 465.3 bits (1196), Expect = 6.4e-131
Identity = 244/508 (48.03%), Postives = 346/508 (68.11%), Query Frame = 0

Query: 18  FPLLAIFLTTLFLISKFRRSEHTKLLLPPSPP-SLPVIGHLHLLKEPFHQMLQDL--SLK 77
           F +L + L+ ++LI K +R  +    LPPSP  SLPVIGHL LLK P H+    L  SL 
Sbjct: 8   FSILFVVLSLIYLIGKLKRKPN----LPPSPAWSLPVIGHLRLLKPPIHRTFLSLSQSLN 67

Query: 78  YGPVFSLTLGFRPLVVVSSPSAVHECFTKNDIVFASRPRMLSGKILNYDYTAVGATAYGQ 137
             P+FSL LG R + V SS S   ECFTKND+V A+RP  +  K + YDYT + A +YG 
Sbjct: 68  NAPIFSLRLGNRLVFVNSSHSIAEECFTKNDVVLANRPNFILAKHVAYDYTTMIAASYGD 127

Query: 138 HWRIMRRIATTELLSTKRLNYFSKLREEEVKLWVKRLYGSVTIREVQKGGRVGLRSKLTE 197
           HWR +RRI + E+ S  RLN F  +R++E++  V RL  + +    Q+  +V ++S L++
Sbjct: 128 HWRNLRRIGSVEIFSNHRLNSFLSIRKDEIRRLVFRLSRNFS----QEFVKVDMKSMLSD 187

Query: 198 LSFNIALMLMAGRRYFGEEVEEGESEEAKELKETMKQVPLLSGASYPADFLSVLKLIDFQ 257
           L+FN  L ++AG+RY+G+ VE  +  EAK +++ +  V   +GA    D+L VL+L+   
Sbjct: 188 LTFNNILRMVAGKRYYGDGVE--DDPEAKRVRQLIADVVACAGAGNAVDYLPVLRLV--S 247

Query: 258 GFRKRLAKVRVDGDAFAQSLVDERRELKGSWCERKTMIDAMLSLQESDPEYYKDDIIKGQ 317
            +  R+ K+    D F Q LVDE+RE K    +  TMID +L+LQES P+Y+ D IIKG 
Sbjct: 248 DYETRVKKLAGRLDEFLQGLVDEKREAKE---KGNTMIDHLLTLQESQPDYFTDRIIKGN 307

Query: 318 FLTLLAAGTDTIASTLEWAMSLLLNHPIVMNKAWTEINQVVGDQRLVREADTNNLHYLQS 377
            L L+ AGTDT A TLEWA+S +LNHP V+NKA  EI++ +G  RL+ E+D +NL YLQ+
Sbjct: 308 MLALILAGTDTSAVTLEWALSNVLNHPDVLNKARDEIDRKIGLDRLMDESDISNLPYLQN 367

Query: 378 IINETYRLFPALPILVPRESSEDCTIGGFDIPKGTMLVVNAWAVHRNPEVWEDPNCFKPE 437
           I++ET RL+PA P+L+P  +SEDC + G+D+P+GT+L+ N WA+HR+P++W+DP  FKPE
Sbjct: 368 IVSETLRLYPAAPMLLPHVASEDCKVAGYDMPRGTILLTNVWAIHRDPQLWDDPMSFKPE 427

Query: 438 RF-KQGETVEVSKLLPFGMGRRACPGAGLAHRVVSLALATLIQCFEWGKLDDKKDVDLCP 497
           RF K+G   E  KL+PFG+GRRACPG+GLAHR+++L L +LIQC EW K+ +  +VD+  
Sbjct: 428 RFEKEG---EAQKLMPFGLGRRACPGSGLAHRLINLTLGSLIQCLEWEKIGE--EVDMSE 487

Query: 498 GIGLTMPKAIPLEAMCKPRASITHLLSQ 522
           G G+TMPKA PLEAMC+ R S+  + ++
Sbjct: 488 GKGVTMPKAKPLEAMCRARPSVVKIFNE 495

BLAST of Cmc07g0183611 vs. TAIR 10
Match: AT4G37330.1 (cytochrome P450, family 81, subfamily D, polypeptide 4 )

HSP 1 Score: 456.4 bits (1173), Expect = 3.0e-128
Identity = 246/503 (48.91%), Postives = 337/503 (67.00%), Query Frame = 0

Query: 21  LAIFLTTLFLISKFRRSEHTKLLLPPSPP-SLPVIGHLHLLKEPFHQMLQDLSLKYG--P 80
           L++F   +FL+++ +R    KL LPPSP  SLPVIGHLHLLK P H+    LS   G  P
Sbjct: 12  LSLFTLAIFLLTRSKR----KLNLPPSPAISLPVIGHLHLLKPPLHRTFLSLSKSIGNAP 71

Query: 81  VFSLTLGFRPLVVVSSPSAVHECFTKNDIVFASRPRMLSGKILNYDYTAVGATAYGQHWR 140
           VF L LG R + V+SS S   ECFTKND+V A+RP+    K L Y+ T + + +YG HWR
Sbjct: 72  VFHLRLGNRLVYVISSRSIAEECFTKNDVVLANRPKFTISKHLGYNATYLLSASYGDHWR 131

Query: 141 IMRRIATTELLSTKRLNYFSKLREEEVKLWVKRLYGSVTIREVQKGGRVGLRSKLTELSF 200
            +RRIA  E+ ST RLN F  +R++E    ++RL   ++   +     V +++ LT L+ 
Sbjct: 132 NLRRIAAVEIFSTHRLNSFLYIRKDE----IRRLISHLSRDSLHGFVEVEMKTLLTNLAS 191

Query: 201 NIALMLMAGRRYFGEEVEEGESEEAKELKETMKQVPLLSGASYPADFLSVLKLIDFQGFR 260
           N  + ++AG+RYFGE     ++++AK +K  + +    +GA  P D+LS+L+ +    + 
Sbjct: 192 NTTIRMLAGKRYFGE-----DNDDAKLVKNLVSEAVTSAGAGNPIDYLSILRWV--SSYE 251

Query: 261 KRLAKVRVDGDAFAQSLVDERRELKGSWCERKTMIDAMLSLQESDPEYYKDDIIKGQFLT 320
           KR+  +    D F Q LVDE+R  K    + +TMID +L+LQ+  P+YY D IIKG  LT
Sbjct: 252 KRIKNLGNRFDTFLQKLVDEKRAEKE---KGETMIDHLLALQDIQPDYYTDVIIKGIILT 311

Query: 321 LLAAGTDTIASTLEWAMSLLLNHPIVMNKAWTEINQVVGDQRLVREADTNNLHYLQSIIN 380
           L+ AGTDT + TLEWAMS LLNHP ++ KA  EI++ VG  RLV E+D  NL YLQSI+ 
Sbjct: 312 LIIAGTDTSSVTLEWAMSNLLNHPEILKKARMEIDEKVGLDRLVDESDIVNLSYLQSIVL 371

Query: 381 ETYRLFPALPILVPRESSEDCTIGGFDIPKGTMLVVNAWAVHRNPEVWEDPNCFKPERF- 440
           ET R++PA+P+L+P  SSEDC +GG+DIP GTM++ NAWA+HR+PEVWEDP  FKPERF 
Sbjct: 372 ETLRMYPAVPLLLPHLSSEDCKVGGYDIPSGTMVLTNAWAMHRDPEVWEDPEIFKPERFE 431

Query: 441 KQGETVEVSKLLPFGMGRRACPGAGLAHRVVSLALATLIQCFEWGKLDDKKDVDLCPGIG 500
           K+G   E  KL+ FGMGRRACPGAGLAHR+++ AL +L+QCFEW ++ +   VD+    G
Sbjct: 432 KEG---EAEKLISFGMGRRACPGAGLAHRLINQALGSLVQCFEWERVGE-DFVDMTEDKG 491

Query: 501 LTMPKAIPLEAMCKPRASITHLL 520
            T+PKAIPL AMCK R+ +  L+
Sbjct: 492 ATLPKAIPLRAMCKARSIVDKLI 492

BLAST of Cmc07g0183611 vs. TAIR 10
Match: AT4G37360.1 (cytochrome P450, family 81, subfamily D, polypeptide 2 )

HSP 1 Score: 443.4 bits (1139), Expect = 2.6e-124
Identity = 237/506 (46.84%), Postives = 336/506 (66.40%), Query Frame = 0

Query: 18  FPLLAIFLTTLFLISKFRRSEHTKLLLPPSPP-SLPVIGHLHLLKEPFHQMLQDLSLKYG 77
           F    I L+ +FLI + +R    KL LPPSP  +LPVIGHL LLK P H++   +S   G
Sbjct: 8   FTFCFIVLSLIFLIGRIKR----KLNLPPSPAWALPVIGHLRLLKPPLHRVFLSVSQSLG 67

Query: 78  --PVFSLTLGFRPLVVVSSPSAVHECFTKNDIVFASRPRMLSGKILNYDYTAVGATAYGQ 137
             P+ SL LG R L VVSS S   ECFTKND++ A+R   +S K ++Y  + V + +Y +
Sbjct: 68  DAPIISLRLGNRLLFVVSSHSIAEECFTKNDVILANRQTTISTKHISYGNSTVVSASYSE 127

Query: 138 HWRIMRRIATTELLSTKRLNYFSKLREEEVKLWVKRLYGSVTIREVQKGGRVGLRSKLTE 197
           HWR +RRI   E+ S  RLN FS +R +E    ++RL G +         +V ++S  ++
Sbjct: 128 HWRNLRRIGALEIFSAHRLNSFSSIRRDE----IRRLIGRLLRNSSYGFTKVEMKSMFSD 187

Query: 198 LSFNIALMLMAGRRYFGEEVEEGESEEAKELKETMKQVPLLSGASYPADFLSVLKLIDFQ 257
           L+FN  + ++AG+ Y+G+  E  +  EAK ++  + +    SG    AD++ +L  I + 
Sbjct: 188 LTFNNIIRMLAGKCYYGDGKE--DDPEAKRVRTLIAEAMSSSGPGNAADYIPILTWITYS 247

Query: 258 GFRKRLAKVRVDGDAFAQSLVDERRELKGSWCERKTMIDAMLSLQESDPEYYKDDIIKGQ 317
               R+ K+    D F Q LVDE+RE  G   +  TM+D +L LQE+ PEYY D IIKG 
Sbjct: 248 --ETRIKKLAGRLDEFLQGLVDEKRE--GKEKKENTMVDHLLCLQETQPEYYMDRIIKGT 307

Query: 318 FLTLLAAGTDTIASTLEWAMSLLLNHPIVMNKAWTEINQVVGDQRLVREADTNNLHYLQS 377
            L+L+A GTDT A TLEWA+S LLN+P V+NKA  EI++++G  RL+ E+D  NL YLQ+
Sbjct: 308 MLSLIAGGTDTTAVTLEWALSSLLNNPEVLNKARDEIDRMIGVDRLLEESDIPNLPYLQN 367

Query: 378 IINETYRLFPALPILVPRESSEDCTIGGFDIPKGTMLVVNAWAVHRNPEVWEDPNCFKPE 437
           I++ET RL+PA P+L+P  +S+DC +GG+D+P+GTML+ NAWA+HR+P +W+DP  FKPE
Sbjct: 368 IVSETLRLYPAAPMLLPHVASKDCKVGGYDMPRGTMLLTNAWAIHRDPLLWDDPTSFKPE 427

Query: 438 RF-KQGETVEVSKLLPFGMGRRACPGAGLAHRVVSLALATLIQCFEWGKLDDKKDVDLCP 497
           RF K+G   E  KL+PFG+GRRACPG+GLA R+V+L+L +LIQCFEW ++ + ++VD+  
Sbjct: 428 RFEKEG---EAKKLMPFGLGRRACPGSGLAQRLVTLSLGSLIQCFEWERIGE-EEVDMTE 487

Query: 498 GIGLTMPKAIPLEAMCKPRASITHLL 520
           G GLTMPKA PLEAMC+ R  +  +L
Sbjct: 488 GPGLTMPKARPLEAMCRARDFVGKIL 495

BLAST of Cmc07g0183611 vs. TAIR 10
Match: AT5G67310.1 (cytochrome P450, family 81, subfamily G, polypeptide 1 )

HSP 1 Score: 436.4 bits (1121), Expect = 3.2e-122
Identity = 240/495 (48.48%), Postives = 317/495 (64.04%), Query Frame = 0

Query: 20  LLAIFLTTLFLISKFRRSEHTKLLLPPSPPSLPVIGHLHLLKEPFHQMLQDLSLKYG-PV 79
           LLA+    + +   FR  +  K  LPP+P   PVIGHLHLLKEP H+ L+DLS   G  V
Sbjct: 20  LLALMAGFIAVAYVFRSKQ--KKNLPPNPVGFPVIGHLHLLKEPVHRSLRDLSRNLGIDV 79

Query: 80  FSLTLGFRPLVVVSSPSAVHECFT-KNDIVFASRPRMLSGKILNYDYTAVGATAYGQHWR 139
           F L LG R  VVV+S SA  E  + +ND+VFA+RP     + + Y+ T V    YG+HWR
Sbjct: 80  FILRLGSRRAVVVTSASAAEEFLSQQNDVVFANRPLATLTEYMGYNNTLVSTAPYGEHWR 139

Query: 140 IMRRIATTELLSTKRLNYFSKLREEEVKLWVKRLYGSVTIREVQKGGRVGLRSK--LTEL 199
            +RR    ++LST RL  FS +R +EV+  +++    + +  V  GG V L+ +  L  L
Sbjct: 140 RLRRFCAVDILSTARLRDFSDIRRDEVRAMIRK----INVELVTSGGSVRLKLQPFLYGL 199

Query: 200 SFNIALMLMAGRRYFGEEVEEGESEEAKELKETMKQVPLLSGASYPADFLSVLKLIDFQG 259
           ++NI + ++AG+R         E EE KE+++ +++V   +G +Y  DFL  LKL D  G
Sbjct: 200 TYNILMSMVAGKR--------EEDEETKEVRKLIREVFDFAGVNYVGDFLPTLKLFDLDG 259

Query: 260 FRKRLAKVRVDGDAFAQSLVDERRELKGSWCERKTMIDAMLSLQESDPEYYKDDIIKGQF 319
           +RKR  K+    D F Q LVDE R+ +G     KTMI  +LSLQES+PE Y DDIIKG  
Sbjct: 260 YRKRAKKLASKLDKFMQKLVDEHRKNRGKAELEKTMITRLLSLQESEPECYTDDIIKGLV 319

Query: 320 LTLLAAGTDTIASTLEWAMSLLLNHPIVMNKAWTEINQVVGDQRLVREADTNNLHYLQSI 379
             +L AGTDT A TLEWAM+ LLNHP V+ K  TE+N+V  + R+  E+DT    YL ++
Sbjct: 320 QVMLLAGTDTTAVTLEWAMANLLNHPEVLRKLKTELNEVSKEGRVFEESDTGKCPYLNNV 379

Query: 380 INETYRLFPALPILVPRESSEDCTIGGFDIPKGTMLVVNAWAVHRNPEVWEDPNCFKPER 439
           I+ET RLFPA P+LVP  SS DC + GFDIP+ T L +NAWA+ R+P VW+DP  FKPER
Sbjct: 380 ISETLRLFPAAPLLVPHASSTDCEVAGFDIPRRTWLFINAWAIQRDPNVWDDPETFKPER 439

Query: 440 FKQGETVEVSKLLPFGMGRRACPGAGLAHRVVSLALATLIQCFEWGKLDDKKDVDLCPGI 499
           F + ET    K LPFG+GRRACPG GLA  V+SLAL +LIQCF+W + D+   VD+  G 
Sbjct: 440 F-ESET-HRGKFLPFGIGRRACPGMGLAQLVLSLALGSLIQCFDWER-DNDVAVDMSEGK 497

Query: 500 GLTMPKAIPLEAMCK 511
           GLTMPK++PL A CK
Sbjct: 500 GLTMPKSVPLVAKCK 497

BLAST of Cmc07g0183611 vs. TAIR 10
Match: AT4G37340.1 (cytochrome P450, family 81, subfamily D, polypeptide 3 )

HSP 1 Score: 434.5 bits (1116), Expect = 1.2e-121
Identity = 243/524 (46.37%), Postives = 337/524 (64.31%), Query Frame = 0

Query: 3   MEQQTSFFFFFSHFNFPLLAIFLTTLFLISKFRRSEHTKLLLPPSPP-SLPVIGHLHLLK 62
           ME QT  F F        L I L+  F+I + +R  +    LPPSP  +LPVIGHL LLK
Sbjct: 1   MEGQTLIFTF--------LFISLSLTFIIGRIKRRPN----LPPSPSWALPVIGHLRLLK 60

Query: 63  EPFHQMLQDLSLKYG--PVFSLTLGFRPLVVVSSPSAVHECFTKNDIVFASRPRMLSGKI 122
            P H++   +S   G  P+ SL LG R + VVSS S   ECFTKND+V A+R   L+ K 
Sbjct: 61  PPLHRVFLSVSESLGDAPIISLRLGNRLVFVVSSHSLAEECFTKNDVVLANRFNSLASKH 120

Query: 123 LNYDYTAVGATAYGQHWRIMRRIATTELLSTKRLNYFSKLREEEVKLWVKRLYGSVTIRE 182
           ++Y  T V   +YG HWR +RRI   E+ S  RLN FS +R +E    + RL   ++   
Sbjct: 121 ISYGCTTVVTASYGDHWRNLRRIGAVEIFSAHRLNSFSSIRRDE----IHRLIACLSRNS 180

Query: 183 VQKGGRVGLRSKLTELSFNIALMLMAGRRYFGEEVEEGESEEAKELKETMKQVPLLSGAS 242
             +  +V ++S  + L+FN  + ++AG+ Y+G+  E  +  EAK ++E + +     GA 
Sbjct: 181 SLEFTKVEMKSMFSNLTFNNIIRMLAGKCYYGDGAE--DDPEAKRVRELIAEGMGCFGAG 240

Query: 243 YPADFLSVLKLIDFQGFRKRLAKVRVDGDAFAQSLVDERRELKGSWCERKTMIDAMLSLQ 302
             AD+L +L  I   G  KR+ K+    D F Q LVDERRE  G    + TM+D +L LQ
Sbjct: 241 NTADYLPILTWI--TGSEKRIKKIASRLDEFLQGLVDERRE--GKEKRQNTMVDHLLCLQ 300

Query: 303 ESDPEYYKDDIIKGQFLTLLAAGTDTIASTLEWAMSLLLNHPIVMNKAWTEINQVVGDQR 362
           E+ PEYY D+IIKG  L+L+ AGTDT A TLEW +S LLNHP +++KA  EI+  VG  R
Sbjct: 301 ETQPEYYTDNIIKGIMLSLILAGTDTSAVTLEWTLSALLNHPQILSKARDEIDNKVGLNR 360

Query: 363 LVREADTNNLHYLQSIINETYRLFPALPILVPRESSEDCTIGGFDIPKGTMLVVNAWAVH 422
           LV E+D ++L YLQ+I++E+ RL+PA P+LVP  +SEDC +GG+ +P+GTML+ NAWA+H
Sbjct: 361 LVEESDLSHLPYLQNIVSESLRLYPASPLLVPHVASEDCKVGGYHMPRGTMLLTNAWAIH 420

Query: 423 RNPEVWEDPNCFKPERF-KQGETVEVSKLLPFGMGRRACPGAGLAHRVVSLALATLIQCF 482
           R+P++W+DP  FKPERF K+G   E  KLL FG+GRRACPG+GLA R+ SL + +LIQCF
Sbjct: 421 RDPKIWDDPTSFKPERFEKEG---EAQKLLGFGLGRRACPGSGLAQRLASLTIGSLIQCF 480

Query: 483 EWGKLDDKKDVDLCP-GIGLTMPKAIPLEAMCKPRASITHLLSQ 522
           EW ++ + ++VD+   G G+ MPKAIPL AMCK R  +  +L++
Sbjct: 481 EWERIGE-EEVDMTEGGGGVIMPKAIPLVAMCKARPVVGKILNE 498

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_008452612.18.8e-30099.23PREDICTED: isoflavone 2'-hydroxylase-like [Cucumis melo] >KAA0064425.1 isoflavon... [more]
XP_004141394.16.2e-27791.98cytochrome P450 81Q32 [Cucumis sativus] >KGN55244.1 hypothetical protein Csa_012... [more]
XP_038900261.14.5e-22780.04LOW QUALITY PROTEIN: cytochrome P450 81Q32-like [Benincasa hispida][more]
XP_022940149.15.0e-22678.43cytochrome P450 81E8-like [Cucurbita moschata][more]
XP_023523499.14.2e-22578.04cytochrome P450 81E8-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
W8JMU77.9e-14252.31Cytochrome P450 81Q32 OS=Catharanthus roseus OX=4058 GN=CYP81Q32 PE=2 SV=1[more]
P931472.7e-12645.92Isoflavone 2'-hydroxylase OS=Glycyrrhiza echinata OX=46348 GN=CYP81E1 PE=1 SV=2[more]
Q6WNQ93.0e-12545.98Isoflavone 3'-hydroxylase (Fragment) OS=Medicago truncatula OX=3880 GN=CYP81E9 P... [more]
Q6WNQ86.9e-12246.76Cytochrome P450 81E8 OS=Medicago truncatula OX=3880 GN=CYP81E8 PE=2 SV=1[more]
Q6WNR01.0e-12043.50Isoflavone 2'-hydroxylase OS=Medicago truncatula OX=3880 GN=CYP81E7 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A5D3D9B54.3e-30099.23Isoflavone 2'-hydroxylase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_s... [more]
A0A1S3BTN14.3e-30099.23isoflavone 2'-hydroxylase-like OS=Cucumis melo OX=3656 GN=LOC103493583 PE=3 SV=1[more]
A0A0A0L2I13.0e-27791.98Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G641760 PE=3 SV=1[more]
A0A6J1FJ782.4e-22678.43cytochrome P450 81E8-like OS=Cucurbita moschata OX=3662 GN=LOC111445861 PE=3 SV=... [more]
A0A6J1J4B31.9e-22377.25isoflavone 3'-hydroxylase-like OS=Cucurbita maxima OX=3661 GN=LOC111481120 PE=3 ... [more]
Match NameE-valueIdentityDescription
AT4G37370.16.4e-13148.03cytochrome P450, family 81, subfamily D, polypeptide 8 [more]
AT4G37330.13.0e-12848.91cytochrome P450, family 81, subfamily D, polypeptide 4 [more]
AT4G37360.12.6e-12446.84cytochrome P450, family 81, subfamily D, polypeptide 2 [more]
AT5G67310.13.2e-12248.48cytochrome P450, family 81, subfamily G, polypeptide 1 [more]
AT4G37340.11.2e-12146.37cytochrome P450, family 81, subfamily D, polypeptide 3 [more]
InterPro
Analysis Name: InterPro Annotations of Melon (Charmono) v1.1
Date Performed: 2022-10-13
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002401Cytochrome P450, E-class, group IPRINTSPR00463EP450Icoord: 330..356
score: 37.96
coord: 373..391
score: 33.45
coord: 457..480
score: 36.66
coord: 447..457
score: 56.31
coord: 72..91
score: 45.83
coord: 414..438
score: 36.91
coord: 96..117
score: 24.89
coord: 310..327
score: 29.73
coord: 195..213
score: 20.01
IPR001128Cytochrome P450PRINTSPR00385P450coord: 448..457
score: 59.91
coord: 321..338
score: 41.42
coord: 374..385
score: 36.02
coord: 457..468
score: 33.58
IPR001128Cytochrome P450PFAMPF00067p450coord: 45..498
e-value: 1.8E-92
score: 310.5
IPR036396Cytochrome P450 superfamilyGENE3D1.10.630.10Cytochrome P450coord: 29..513
e-value: 5.0E-120
score: 403.3
IPR036396Cytochrome P450 superfamilySUPERFAMILY48264Cytochrome P450coord: 45..512
NoneNo IPR availablePIRSRPIRSR000047-1PIRSR000047-1coord: 22..489
e-value: 6.4E-18
score: 62.7
NoneNo IPR availablePANTHERPTHR24298FLAVONOID 3'-MONOOXYGENASE-RELATEDcoord: 25..517
NoneNo IPR availablePANTHERPTHR24298:SF683CYTOCHROME P450 FAMILY PROTEINcoord: 25..517
IPR017972Cytochrome P450, conserved sitePROSITEPS00086CYTOCHROME_P450coord: 450..459

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cmc07g0183611.1Cmc07g0183611.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016020 membrane
molecular_function GO:0020037 heme binding
molecular_function GO:0005506 iron ion binding
molecular_function GO:0016709 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NAD(P)H as one donor, and incorporation of one atom of oxygen
molecular_function GO:0004497 monooxygenase activity
molecular_function GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen