Homology
BLAST of Cmc07g0183611 vs. NCBI nr
Match:
XP_008452612.1 (PREDICTED: isoflavone 2'-hydroxylase-like [Cucumis melo] >KAA0064425.1 isoflavone 2'-hydroxylase-like [Cucumis melo var. makuwa] >TYK20162.1 isoflavone 2'-hydroxylase-like [Cucumis melo var. makuwa])
HSP 1 Score: 1039.6 bits (2687), Expect = 8.8e-300
Identity = 518/522 (99.23%), Postives = 520/522 (99.62%), Query Frame = 0
Query: 1 MDMEQQTSFFFFFSHFNFPLLAIFLTTLFLISKFRRSEHTKLLLPPSPPSLPVIGHLHLL 60
MDMEQQTSFFFFFSHFNFPLLAIFLTTLFLISKFRRSEHTKLLLPPSPPSLPVIGHLHLL
Sbjct: 1 MDMEQQTSFFFFFSHFNFPLLAIFLTTLFLISKFRRSEHTKLLLPPSPPSLPVIGHLHLL 60
Query: 61 KEPFHQMLQDLSLKYGPVFSLTLGFRPLVVVSSPSAVHECFTKNDIVFASRPRMLSGKIL 120
KEPFHQMLQDLSLKYGPVFSLTLGFRPLVVVSSPSAVHECFTKNDIVFASRPRMLSGKIL
Sbjct: 61 KEPFHQMLQDLSLKYGPVFSLTLGFRPLVVVSSPSAVHECFTKNDIVFASRPRMLSGKIL 120
Query: 121 NYDYTAVGATAYGQHWRIMRRIATTELLSTKRLNYFSKLREEEVKLWVKRLYGSVTIREV 180
NYDYTAVGATAYGQHWRIMRRIATTELLSTKRLN FSKLREEEVKLWVKRLYGSVTIREV
Sbjct: 121 NYDYTAVGATAYGQHWRIMRRIATTELLSTKRLNDFSKLREEEVKLWVKRLYGSVTIREV 180
Query: 181 QKGGRVGLRSKLTELSFNIALMLMAGRRYFGEEVEEGESEEAKELKETMKQVPLLSGASY 240
QKGGRVGLRSKLTELSFNIALML AGRRYFGEEVEEGESEEAKELKETMKQVPLLSGASY
Sbjct: 181 QKGGRVGLRSKLTELSFNIALMLTAGRRYFGEEVEEGESEEAKELKETMKQVPLLSGASY 240
Query: 241 PADFLSVLKLIDFQGFRKRLAKVRVDGDAFAQSLVDERRELKGSWCERKTMIDAMLSLQE 300
PADFLSVLKLIDFQGFRKRLAKVRVDGDAFAQSLVDERRELKGSWCERKTMIDAMLSLQE
Sbjct: 241 PADFLSVLKLIDFQGFRKRLAKVRVDGDAFAQSLVDERRELKGSWCERKTMIDAMLSLQE 300
Query: 301 SDPEYYKDDIIKGQFLTLLAAGTDTIASTLEWAMSLLLNHPIVMNKAWTEINQVVGDQRL 360
SDPEYYKDDIIKGQFLTLLAAGTDTIASTLEWAMSLLLNHPIVMNKAWTEINQVVGDQRL
Sbjct: 301 SDPEYYKDDIIKGQFLTLLAAGTDTIASTLEWAMSLLLNHPIVMNKAWTEINQVVGDQRL 360
Query: 361 VREADTNNLHYLQSIINETYRLFPALPILVPRESSEDCTIGGFDIPKGTMLVVNAWAVHR 420
VREADTNNLHYLQSIINETYRLFPALPILVPRESSEDCTIGGFDIPKGTMLVVNAWAVHR
Sbjct: 361 VREADTNNLHYLQSIINETYRLFPALPILVPRESSEDCTIGGFDIPKGTMLVVNAWAVHR 420
Query: 421 NPEVWEDPNCFKPERFKQGETVEVSKLLPFGMGRRACPGAGLAHRVVSLALATLIQCFEW 480
NPEVWEDPNCFKPERFKQGETVEVSKLLPFGMGRRACPGAGLAHRVVSLALATLIQCFEW
Sbjct: 421 NPEVWEDPNCFKPERFKQGETVEVSKLLPFGMGRRACPGAGLAHRVVSLALATLIQCFEW 480
Query: 481 GKLDDKKDVDLCPGIGLTMPKAIPLEAMCKPRASITHLLSQL 523
GKLD+KKDVDLCPGIGLTMPKAIPLEAMCKPRAS+THLLSQL
Sbjct: 481 GKLDNKKDVDLCPGIGLTMPKAIPLEAMCKPRASVTHLLSQL 522
BLAST of Cmc07g0183611 vs. NCBI nr
Match:
XP_004141394.1 (cytochrome P450 81Q32 [Cucumis sativus] >KGN55244.1 hypothetical protein Csa_012467 [Cucumis sativus])
HSP 1 Score: 963.8 bits (2490), Expect = 6.2e-277
Identity = 482/524 (91.98%), Postives = 499/524 (95.23%), Query Frame = 0
Query: 3 MEQQTSFFFFFSH----FNFPLLAIFLTTLFLISKFRRSEHTKLLLPPSPPSLPVIGHLH 62
MEQQTSFFF H + PLLAIFLT+LFLISKFRR++HTK LLPPSPPSLPVIGHLH
Sbjct: 1 MEQQTSFFFSQDHHFHPISIPLLAIFLTSLFLISKFRRTQHTK-LLPPSPPSLPVIGHLH 60
Query: 63 LLKEPFHQMLQDLSLKYGPVFSLTLGFRPLVVVSSPSAVHECFTKNDIVFASRPRMLSGK 122
LLKEPFHQMLQD S KYGPVFSLTLGFRPLVVVSSPSAV ECFTKNDIVFASRPRMLSGK
Sbjct: 61 LLKEPFHQMLQDFSKKYGPVFSLTLGFRPLVVVSSPSAVQECFTKNDIVFASRPRMLSGK 120
Query: 123 ILNYDYTAVGATAYGQHWRIMRRIATTELLSTKRLNYFSKLREEEVKLWVKRLYGSVTIR 182
ILNYDYTAVGATAYGQHWRIMRRIATTELLSTKRLN SK+REEEVKLWVKRLYGSVT
Sbjct: 121 ILNYDYTAVGATAYGQHWRIMRRIATTELLSTKRLNDLSKIREEEVKLWVKRLYGSVTRA 180
Query: 183 EVQKGGRVGLRSKLTELSFNIALMLMAGRRYFGEEVEEGESEEAKELKETMKQVPLLSGA 242
EV KGGRVGLRSKLTELSFNIALMLMAGRRYFG+E+E GESEEAKELKETMKQVPLLSGA
Sbjct: 181 EVNKGGRVGLRSKLTELSFNIALMLMAGRRYFGDEMEAGESEEAKELKETMKQVPLLSGA 240
Query: 243 SYPADFLSVLKLIDFQGFRKRLAKVRVDGDAFAQSLVDERRELKGSWCERKTMIDAMLSL 302
SYPADFLSVLKLIDFQGFRKRLAKVRVDGDAFAQSLVDERR+LKGSWCERKTMID+ML L
Sbjct: 241 SYPADFLSVLKLIDFQGFRKRLAKVRVDGDAFAQSLVDERRKLKGSWCERKTMIDSMLCL 300
Query: 303 QESDPEYYKDDIIKGQFLTLLAAGTDTIASTLEWAMSLLLNHPIVMNKAWTEINQVVGDQ 362
QESDPEYYKDDIIKGQFLTLLAAGTDT ASTLEWAMSLLLNHP V+ KAWTEINQVVGDQ
Sbjct: 301 QESDPEYYKDDIIKGQFLTLLAAGTDTTASTLEWAMSLLLNHPTVLEKAWTEINQVVGDQ 360
Query: 363 RLVREADTNNLHYLQSIINETYRLFPALPILVPRESSEDCTIGGFDIPKGTMLVVNAWAV 422
RL+READTN LHYLQ+IINETYRLFPALPILVPRESSEDCTIGGFDIPKGTMLVVNAWAV
Sbjct: 361 RLIREADTNKLHYLQAIINETYRLFPALPILVPRESSEDCTIGGFDIPKGTMLVVNAWAV 420
Query: 423 HRNPEVWEDPNCFKPERFKQGETVEVSKLLPFGMGRRACPGAGLAHRVVSLALATLIQCF 482
HR+P VWEDPNCFKPERFKQGETVEV+KLLPFGMGRRACPGAGLAHRVVSLALATLIQCF
Sbjct: 421 HRDPNVWEDPNCFKPERFKQGETVEVNKLLPFGMGRRACPGAGLAHRVVSLALATLIQCF 480
Query: 483 EWGKLDDKKDVDLCPGIGLTMPKAIPLEAMCKPRASITHLLSQL 523
EWGKLD++KDVDLCPGIGLTMPKAIPLEAMCKPRAS+THLLSQL
Sbjct: 481 EWGKLDNQKDVDLCPGIGLTMPKAIPLEAMCKPRASVTHLLSQL 523
BLAST of Cmc07g0183611 vs. NCBI nr
Match:
XP_038900261.1 (LOW QUALITY PROTEIN: cytochrome P450 81Q32-like [Benincasa hispida])
HSP 1 Score: 798.1 bits (2060), Expect = 4.5e-227
Identity = 417/521 (80.04%), Postives = 446/521 (85.60%), Query Frame = 0
Query: 3 MEQQTSFFFFFSHF-NFPLLAIFLTTLFLISKFRRSEHTKLLLPPSPPSLPVIGHLHLLK 62
ME+Q S FFFSHF PL A+ +TTLFL+SKFR++EH K LPPSPPSLPVIGHLHLLK
Sbjct: 1 MEEQAS--FFFSHFPTIPLFALLITTLFLLSKFRKTEHRK--LPPSPPSLPVIGHLHLLK 60
Query: 63 EPFHQMLQDLSLKYGPVFSLTLGFRPLVVVSSPSAVHECFTKNDIVFASRPRMLSGKILN 122
EPFHQMLQDLS KYGPVFSLTLGFRP+VVVS+PSAV E KILN
Sbjct: 61 EPFHQMLQDLSKKYGPVFSLTLGFRPVVVVSAPSAVQE-----------------WKILN 120
Query: 123 YDYTAVGATAYGQHWRIMRRIATTELLSTKRLNYFSKLREEEVKLWVKRLYGSVTIREVQ 182
YDYTAVGATAYGQHWRIMRRIATTELLST RLN SK+REEEVKLWVKRLYG + E
Sbjct: 121 YDYTAVGATAYGQHWRIMRRIATTELLSTHRLNSLSKIREEEVKLWVKRLYGMTEVGESN 180
Query: 183 KGGRVGLRSKLTELSFNIALMLMAGRRYFGEEVEEGESEEAKELKETMKQVPLLSGASYP 242
KG RVGLRSKLTELSFNIALMLM GRRYFG EV EGESEEAKELKETMKQVPLLSGASYP
Sbjct: 181 KGVRVGLRSKLTELSFNIALMLMTGRRYFGGEV-EGESEEAKELKETMKQVPLLSGASYP 240
Query: 243 ADFLSVLKLIDFQGFRKRLAKVRVDGDAFAQSLVDERRELKGSWCERKTMIDAMLSLQES 302
ADFLSVLK +DFQ VRVDGDAFAQS+VDERR+LKGS CERKT+ID++L LQES
Sbjct: 241 ADFLSVLKWVDFQ--------VRVDGDAFAQSVVDERRKLKGS-CERKTIIDSLLLLQES 300
Query: 303 DPEYYKDDIIKGQFLTLLAAGTDTIASTLEWAMSLLLNHPIVMNKAWTEINQVVGDQRLV 362
DPEYYKD+IIKGQFLTLLAAGTDT A TLEWAMSLLLNHP M KAWTEIN+VVGDQRLV
Sbjct: 301 DPEYYKDEIIKGQFLTLLAAGTDTTARTLEWAMSLLLNHPTEMKKAWTEINEVVGDQRLV 360
Query: 363 READTNNLHYLQSIINETYRLFPALPILVPRESSEDCTIGGFDIPKGTMLVVNAWAVHRN 422
EAD N HYLQ+IINETYRLFPALPIL PRESSEDCTIGGFDIPKGTMLVVNAWAVHR+
Sbjct: 361 EEADRNKPHYLQAIINETYRLFPALPILAPRESSEDCTIGGFDIPKGTMLVVNAWAVHRD 420
Query: 423 PEVWEDPNCFKPERFKQGETVEVSKLLPFGMGRRACPGAGLAHRVVSLALATLIQCFEWG 482
P VWE PN F+PERFK G+ +EVSKLLPFGMGRRA PGAGLAHRVV+LALATLIQCFEWG
Sbjct: 421 PNVWEHPNSFQPERFKPGQXLEVSKLLPFGMGRRAGPGAGLAHRVVALALATLIQCFEWG 480
Query: 483 KLDDKKDVDLCPGIGLTMPKAIPLEAMCKPRASITHLLSQL 523
+ D+K +DL PGIGLTMP+AIPLEA+CKPRASITHLLSQL
Sbjct: 481 R-PDQKGLDLSPGIGLTMPRAIPLEAICKPRASITHLLSQL 489
BLAST of Cmc07g0183611 vs. NCBI nr
Match:
XP_022940149.1 (cytochrome P450 81E8-like [Cucurbita moschata])
HSP 1 Score: 794.7 bits (2051), Expect = 5.0e-226
Identity = 400/510 (78.43%), Postives = 442/510 (86.67%), Query Frame = 0
Query: 13 FSHFNFPLLAIFLTTLFLISKFRRSEHTKLLLPPSPPSLPVIGHLHLLKEPFHQMLQDLS 72
FS+F PLL + L L LI KFR++EH LPP PPSLPVIGHLHLLKEPFHQMLQDLS
Sbjct: 11 FSNFEVPLLVLIL-ALLLIFKFRKAEHKN--LPPGPPSLPVIGHLHLLKEPFHQMLQDLS 70
Query: 73 LKYGPVFSLTLGFRPLVVVSSPSAVHECFTKNDIVFASRPRMLSGKILNYDYTAVGATAY 132
KYGP+ SL+LGFRP+VVVSSP+A ECF+KNDI+FASRPRM+SGKILNYDYTAVGATAY
Sbjct: 71 QKYGPILSLSLGFRPVVVVSSPAAAQECFSKNDIIFASRPRMISGKILNYDYTAVGATAY 130
Query: 133 GQHWRIMRRIATTELLSTKRLNYFSKLREEEVKLWVKRLYGSVTIREVQKGGRVGLRSKL 192
GQHWRIMRRIATTELLST RLN +R++EVKLW+K LY V RV +RSKL
Sbjct: 131 GQHWRIMRRIATTELLSTYRLNALINIRQQEVKLWLKSLYRISGKNNV----RVEIRSKL 190
Query: 193 TELSFNIALMLMAGRRYFGEEVEEGESEEAKELKETMKQVPLLSGASYPADFLSVLKLID 252
TELSFNIALMLM G+RYFG+E + +SEEA+ELK TMKQVPLLSGASYPADFLSVLKL+D
Sbjct: 191 TELSFNIALMLMVGKRYFGDEAVD-DSEEARELKNTMKQVPLLSGASYPADFLSVLKLVD 250
Query: 253 FQGFRKRLAKVRVDGDAFAQSLVDERRELKGSWCERKTMIDAMLSLQESDPEYYKDDIIK 312
+QGFRKRLAKVRVDGDAFAQSL+DERR K ERKTMID++LS+Q+ +PEYYKDDIIK
Sbjct: 251 YQGFRKRLAKVRVDGDAFAQSLIDERRNKKADDNERKTMIDSLLSMQQMEPEYYKDDIIK 310
Query: 313 GQFLTLLAAGTDTIASTLEWAMSLLLNHPIVMNKAWTEINQVVGDQRLVREADTNNLHYL 372
GQFLTLLAAGTDT A TLEWA+SLLLNHP M KAWTEIN+ VGD+RLV E D N LHYL
Sbjct: 311 GQFLTLLAAGTDTTAGTLEWALSLLLNHPTAMKKAWTEINEHVGDERLVEEMDLNQLHYL 370
Query: 373 QSIINETYRLFPALPILVPRESSEDCTIGGFDIPKGTMLVVNAWAVHRNPEVWEDPNCFK 432
Q IINETYRLFPALPILVPRESSEDCTIGGFD+PKGTMLVVNAWA+HR+P+VWEDPN F+
Sbjct: 371 QGIINETYRLFPALPILVPRESSEDCTIGGFDVPKGTMLVVNAWAIHRDPKVWEDPNSFR 430
Query: 433 PERFKQGETVEVSKLLPFGMGRRACPGAGLAHRVVSLALATLIQCFEWGKLDDKKDVDLC 492
PERFK+GE+VEVSKLLPFGMGRRACPGAGLAHRVV LALATLIQCFEW +LD+ K+VDL
Sbjct: 431 PERFKEGESVEVSKLLPFGMGRRACPGAGLAHRVVGLALATLIQCFEWERLDN-KEVDLT 490
Query: 493 PGIGLTMPKAIPLEAMCKPRASITHLLSQL 523
PGIGLTMPKA PL AM KPRA I HLLSQL
Sbjct: 491 PGIGLTMPKARPLVAMFKPRAPIIHLLSQL 511
BLAST of Cmc07g0183611 vs. NCBI nr
Match:
XP_023523499.1 (cytochrome P450 81E8-like [Cucurbita pepo subsp. pepo])
HSP 1 Score: 791.6 bits (2043), Expect = 4.2e-225
Identity = 398/510 (78.04%), Postives = 440/510 (86.27%), Query Frame = 0
Query: 13 FSHFNFPLLAIFLTTLFLISKFRRSEHTKLLLPPSPPSLPVIGHLHLLKEPFHQMLQDLS 72
FS+F PLL + L L LI KFR+++H LPP PPSLPVIGHLHLLKEPFHQMLQDLS
Sbjct: 11 FSNFEVPLLVLIL-ALLLILKFRKADHKN--LPPGPPSLPVIGHLHLLKEPFHQMLQDLS 70
Query: 73 LKYGPVFSLTLGFRPLVVVSSPSAVHECFTKNDIVFASRPRMLSGKILNYDYTAVGATAY 132
KYGP+ SL+LGFRP+VVVSSP+A ECF+KNDI+FASRPRM+SGKILNYDYTAVGATAY
Sbjct: 71 HKYGPILSLSLGFRPVVVVSSPAAAQECFSKNDIIFASRPRMISGKILNYDYTAVGATAY 130
Query: 133 GQHWRIMRRIATTELLSTKRLNYFSKLREEEVKLWVKRLYGSVTIREVQKGGRVGLRSKL 192
GQHWRIMRRIATTELLST RLN +R++EVKLW+K LY V RV +RSKL
Sbjct: 131 GQHWRIMRRIATTELLSTYRLNALINIRQQEVKLWLKSLYRISGKNNV----RVEIRSKL 190
Query: 193 TELSFNIALMLMAGRRYFGEEVEEGESEEAKELKETMKQVPLLSGASYPADFLSVLKLID 252
TELSFNIALMLM G+RYFG+E + +SEEA+ELK TMKQVPLLSGASYPADFLSVLKL+D
Sbjct: 191 TELSFNIALMLMVGKRYFGDEAVD-DSEEARELKNTMKQVPLLSGASYPADFLSVLKLVD 250
Query: 253 FQGFRKRLAKVRVDGDAFAQSLVDERRELKGSWCERKTMIDAMLSLQESDPEYYKDDIIK 312
+QGFRKRLAKVRVDGDAFAQSL+DERR K ERKTMID++LS+Q+ +PEYYKDDIIK
Sbjct: 251 YQGFRKRLAKVRVDGDAFAQSLIDERRNKKADDNERKTMIDSLLSMQQMEPEYYKDDIIK 310
Query: 313 GQFLTLLAAGTDTIASTLEWAMSLLLNHPIVMNKAWTEINQVVGDQRLVREADTNNLHYL 372
GQFLTLLAAGTDT A TLEWA+SLLLNHP M KAWTEIN+ VGD+RLV E D N LHYL
Sbjct: 311 GQFLTLLAAGTDTTAGTLEWALSLLLNHPTAMKKAWTEINEHVGDERLVEEMDLNQLHYL 370
Query: 373 QSIINETYRLFPALPILVPRESSEDCTIGGFDIPKGTMLVVNAWAVHRNPEVWEDPNCFK 432
Q IINETYRLFPALPILVPRESSEDCTIGGFD+PKGTMLVVNAWA+HR+P+VWEDPN F+
Sbjct: 371 QGIINETYRLFPALPILVPRESSEDCTIGGFDVPKGTMLVVNAWAIHRDPKVWEDPNSFR 430
Query: 433 PERFKQGETVEVSKLLPFGMGRRACPGAGLAHRVVSLALATLIQCFEWGKLDDKKDVDLC 492
PERFK+GE+VEVSKLLPFGMGRRACPGAGLAHRVV LALATLIQCFEW +L D K+VDL
Sbjct: 431 PERFKEGESVEVSKLLPFGMGRRACPGAGLAHRVVGLALATLIQCFEWERL-DSKEVDLT 490
Query: 493 PGIGLTMPKAIPLEAMCKPRASITHLLSQL 523
PGIGLTMPKA PL AM KPR I HLLSQL
Sbjct: 491 PGIGLTMPKASPLVAMFKPRTPIIHLLSQL 511
BLAST of Cmc07g0183611 vs. ExPASy Swiss-Prot
Match:
W8JMU7 (Cytochrome P450 81Q32 OS=Catharanthus roseus OX=4058 GN=CYP81Q32 PE=2 SV=1)
HSP 1 Score: 505.4 bits (1300), Expect = 7.9e-142
Identity = 272/520 (52.31%), Postives = 359/520 (69.04%), Query Frame = 0
Query: 3 MEQQTSFFFFFSHFNFPLLAIFLTTLFLISKFRRSEHTKLLLPPSPP-SLPVIGHLHLLK 62
ME T + F + LL+I L LF +S+ RR+ LPPSP +LPVIGHLHL+
Sbjct: 1 MESSTLLYTFLA---VVLLSISL-KLFPVSRRRRN------LPPSPGLALPVIGHLHLIG 60
Query: 63 EPFHQMLQDLSLKYGPVFSLTLGFRPLVVVSSPSAVHECFTKNDIVFASRPRMLSGKILN 122
+ H+ L DLS KYG VFSL LG R ++VVSSP+A ECFTKNDIVFA+RP + GK +
Sbjct: 61 KLLHRSLYDLSKKYGSVFSLQLGNRLVLVVSSPAAAEECFTKNDIVFANRPLFILGKYIG 120
Query: 123 YDYTAVGATAYGQHWRIMRRIATTELLSTKRLNYFSKLREEEVKLWVKRLYGSVTIREVQ 182
Y+YT + + YG+HWR +RR+A E+ S LN F +RE+EVK + LY S Q
Sbjct: 121 YNYTTMVGSPYGEHWRNLRRLAAVEIFSAGSLNRFLSIREDEVKQLLLSLYQS----SGQ 180
Query: 183 KGGRVGLRSKLTELSFNIALMLMAGRRYFGEEVEEGESEEAKELKETMKQVPLLSGASYP 242
G+V ++SKL+ELSFN+ + ++AG+RYFG++V +S+EAK + + +V +GAS P
Sbjct: 181 DFGKVEMKSKLSELSFNVTMRMVAGKRYFGQDV---DSDEAKLFRALIGEVFEHAGASNP 240
Query: 243 ADFLSVLKLIDFQGFRKRLAKVRVDGDAFAQSLVDERRELKGSWCERKTMIDAMLSLQES 302
DF+ L+ IDF+ + K+++K+ + DAF Q L+ E R K + TMID +LSLQES
Sbjct: 241 GDFVPFLRWIDFKNYEKKVSKISQEMDAFLQRLIHESRINKNN----VTMIDHLLSLQES 300
Query: 303 DPEYYKDDIIKGQFLTLLAAGTDTIASTLEWAMSLLLNHPIVMNKAWTEINQVVGDQRLV 362
PEYY D IIKG + LL AGTDT A T+EWAMSLLLNHP + KA TEI VG RL+
Sbjct: 301 QPEYYTDQIIKGIIMVLLLAGTDTSAVTVEWAMSLLLNHPETLEKARTEIETQVGSNRLI 360
Query: 363 READTNNLHYLQSIINETYRLFPALPILVPRESSEDCTIGGFDIPKGTMLVVNAWAVHRN 422
E D L YL +II+ET+RL PA P+LVP ESS+DC + G+D+PKGT+L+VNAWA+HR+
Sbjct: 361 EEQDLPKLTYLHNIISETFRLCPAAPMLVPHESSDDCKVQGYDVPKGTILLVNAWAIHRD 420
Query: 423 PEVWEDPNCFKPERFKQGETVEVSKLLPFGMGRRACPGAGLAHRVVSLALATLIQCFEWG 482
PE W++P FKPER G +E SKL+PFGMGRR+CPG+GLA RVV L L LIQCFEW
Sbjct: 421 PEFWDEPTLFKPER-HGGVELEPSKLMPFGMGRRSCPGSGLAQRVVGLTLGALIQCFEWK 480
Query: 483 KLDDKKDVDLCPGIGLTMPKAIPLEAMCKPRASITHLLSQ 522
++ + K +D+ G GLTMPKA PLEA+CKPR + ++S+
Sbjct: 481 RIGEAK-IDMAEGSGLTMPKAQPLEALCKPRNILHKVVSE 497
BLAST of Cmc07g0183611 vs. ExPASy Swiss-Prot
Match:
P93147 (Isoflavone 2'-hydroxylase OS=Glycyrrhiza echinata OX=46348 GN=CYP81E1 PE=1 SV=2)
HSP 1 Score: 453.8 bits (1166), Expect = 2.7e-126
Identity = 231/503 (45.92%), Postives = 329/503 (65.41%), Query Frame = 0
Query: 22 AIFLTTLFLISKFRRSEHTKLLLPPSPPSLPVIGHLHLLKEPFHQMLQDLSLKYGPVFSL 81
++F LF I LPP PPSLP+IG+LH LK P H+ + LS KYG VFSL
Sbjct: 10 SVFYLALFFIFNIVIRARKFKNLPPGPPSLPIIGNLHHLKRPLHRTFKGLSEKYGHVFSL 69
Query: 82 TLGFRPLVVVSSPSAVHECFTKNDIVFASRPRMLSGKILNYDYTAVGATAYGQHWRIMRR 141
G R +VVVSS S +CFTKND+V A+RPR LSGK + Y+YT +G+T+YG+HWR +RR
Sbjct: 70 WFGSRLVVVVSSASEFQQCFTKNDVVLANRPRFLSGKYIFYNYTTLGSTSYGEHWRNLRR 129
Query: 142 IATTELLSTKRLNYFSKLREEEVKLWVKRLY--GSVTIREVQKGGRVGLRSKLTELSFNI 201
I ++LS R+N FS +R +E + + RL S E++ L S+L +++FN
Sbjct: 130 ITALDVLSNHRINSFSGIRRDETQRLITRLADDSSTNFAEME------LSSRLYDMTFNN 189
Query: 202 ALMLMAGRRYFGEEVEEGESEEAKELKETMKQVPLLSGASYPADFLSVLKLIDFQGFRKR 261
+ +++G+RY+GE+ + + +EA + ++ + ++ LSGA+ DF+ +L+ +DF+ KR
Sbjct: 190 IMRMISGKRYYGEDCDTSDLQEASQFRDMVSELLQLSGANNKTDFMPLLRFLDFENLEKR 249
Query: 262 LAKVRVDGDAFAQSLVDERRELKGSWCERKTMIDAMLSLQESDPEYYKDDIIKGQFLTLL 321
L + DAF + L++E R K TMID +L+LQ+S PEYY D IIKG L +L
Sbjct: 250 LKDISGKTDAFLRGLIEEHRTKKE---RANTMIDHLLNLQDSQPEYYTDQIIKGLALAML 309
Query: 322 AAGTDTIASTLEWAMSLLLNHPIVMNKAWTEINQVVGDQRLVREADTNNLHYLQSIINET 381
AGTD+ A TLEW+MS LLNHP V+ K E++ VG RLV E+D L YL+++INET
Sbjct: 310 LAGTDSSAVTLEWSMSNLLNHPEVLKKVKDELDTHVGQDRLVDESDLPKLTYLKNVINET 369
Query: 382 YRLFPALPILVPRESSEDCTIGGFDIPKGTMLVVNAWAVHRNPEVWEDPNCFKPERF-KQ 441
RL+ P+L+P +S++C IGG+ +P+ T++++NAWA+HR+PE+W + FKPERF K+
Sbjct: 370 LRLYTPAPLLLPHSTSDECNIGGYKVPQDTIVLINAWAIHRDPELWTEATTFKPERFEKK 429
Query: 442 GETVEVSKLLPFGMGRRACPGAGLAHRVVSLALATLIQCFEWGKLDDKKDVDLCPGIGLT 501
G E+ KL+ FGMGRRACPG GLA R +S+ LA LIQCF+W KL + +DL G T
Sbjct: 430 G---ELEKLIAFGMGRRACPGEGLAIRAISMTLALLIQCFDW-KLINGDKIDLAERDGFT 489
Query: 502 MPKAIPLEAMCKPRASITHLLSQ 522
+ K +PL+AMCK R I + Q
Sbjct: 490 LTKLVPLKAMCKSRPVINKVFKQ 499
BLAST of Cmc07g0183611 vs. ExPASy Swiss-Prot
Match:
Q6WNQ9 (Isoflavone 3'-hydroxylase (Fragment) OS=Medicago truncatula OX=3880 GN=CYP81E9 PE=1 SV=1)
HSP 1 Score: 450.3 bits (1157), Expect = 3.0e-125
Identity = 229/498 (45.98%), Postives = 328/498 (65.86%), Query Frame = 0
Query: 16 FNFPLLAI-FLTTLFLISKF--RRSEHTKLLLPPSPPSLPVIGHLHLLKEPFHQMLQDLS 75
F + LL++ F+ T+ ++ K RR ++ LPP PP++P+IG+LH LK P H+ LS
Sbjct: 3 FYYSLLSLSFIITIKILLKITSRRLKN----LPPGPPTIPIIGNLHHLKHPLHRTFTTLS 62
Query: 76 LKYGPVFSLTLGFRPLVVVSSPSAVHECFTKNDIVFASRPRMLSGKILNYDYTAVGATAY 135
YG +FSL G R +VVVSSPS HECFTKNDI+ A+RPR L+GK + Y+YT +G+ +Y
Sbjct: 63 QTYGDIFSLWFGSRLVVVVSSPSLAHECFTKNDIILANRPRFLTGKYIFYNYTTLGSASY 122
Query: 136 GQHWRIMRRIATTELLSTKRLNYFSKLREEEVKLWVKRLYGSVTIREVQKGGRVGLRSKL 195
G HWR +RRI T ++LS RLN F +R +E +++L V + E +V LR +L
Sbjct: 123 GDHWRNLRRITTIDVLSNNRLNSFLGVRRDETNRLIQKLLKDV-VSEGFGFTKVELRPRL 182
Query: 196 TELSFNIALMLMAGRRYFGEEVEEGESEEAKELKETMKQVPLLSGASYPADFLSVLKLID 255
TE++FN + +++G+RY+G++ + + EEAK+ +E + ++ L GA+ DFL +L+++D
Sbjct: 183 TEMTFNAMMRMISGKRYYGDDGDVSDVEEAKQFREIISEMMSLLGANNKGDFLPLLRVVD 242
Query: 256 FQGFRKRLAKVRVDGDAFAQSLVDERRELKGSWCERKTMIDAMLSLQESDPEYYKDDIIK 315
KR ++ +AF + L++E R + TMID +L L ES PEYY D +IK
Sbjct: 243 LDNLEKRCKRIAKRSNAFLEGLIEEHRR-GNIHSDGGTMIDHLLKLSESQPEYYSDHLIK 302
Query: 316 GQFLTLLAAGTDTIASTLEWAMSLLLNHPIVMNKAWTEINQVVGDQRLVREADTNNLHYL 375
G +L AGTDT A T+EW MS LLNHP V+ KA E++ +G +LV E D + L YL
Sbjct: 303 GLIQGMLLAGTDTSAVTIEWVMSELLNHPEVLKKAKEELDTQIGKNKLVDEQDLSKLPYL 362
Query: 376 QSIINETYRLFPALPILVPRESSEDCTIGGFDIPKGTMLVVNAWAVHRNPEVWEDPNCFK 435
Q+II+ET RL P P+L+P SSEDCTIG F++PK T+++ N W +HR+P+ W D FK
Sbjct: 363 QNIISETLRLHPPAPLLLPHYSSEDCTIGEFNVPKDTIILTNVWGIHRDPKHWNDALSFK 422
Query: 436 PERFKQGETVEVSKLLPFGMGRRACPGAGLAHRVVSLALATLIQCFEWGKLDDKKDVDLC 495
PERF++ E EV+K++ FG+GRRACPG LA R V + LIQCFEW + ++K +D+
Sbjct: 423 PERFEKEE--EVNKVMAFGLGRRACPGLSLAQRTVGFTVGLLIQCFEWERESEEK-LDMM 482
Query: 496 PGIGLTMPKAIPLEAMCK 511
G G+TMP IPL AMCK
Sbjct: 483 EGKGITMPMKIPLRAMCK 491
BLAST of Cmc07g0183611 vs. ExPASy Swiss-Prot
Match:
Q6WNQ8 (Cytochrome P450 81E8 OS=Medicago truncatula OX=3880 GN=CYP81E8 PE=2 SV=1)
HSP 1 Score: 439.1 bits (1128), Expect = 6.9e-122
Identity = 231/494 (46.76%), Postives = 313/494 (63.36%), Query Frame = 0
Query: 20 LLAIFLTTLFLISKFRRSEHTKLLLPPSPPSLPVIGHLHLLKEPFHQMLQDLSLKYGPVF 79
++++F + L F S K LPP P LP+IG+LH LK+P H LS KYG +F
Sbjct: 9 IISLFFLIITLKVFFNTSRKFK-NLPPGPQCLPIIGNLHQLKQPLHHTFHTLSQKYGQIF 68
Query: 80 SLTLGFRPLVVVSSPSAVHECFTKNDIVFASRPRMLSGKILNYDYTAVGATAYGQHWRIM 139
SL G R +VVVSS + ECFTKNDIV A+RP L+GK + Y+ T V + YG HWR +
Sbjct: 69 SLWFGSRLVVVVSSLTIAQECFTKNDIVLANRPHFLTGKYIGYNNTTVAQSPYGDHWRNL 128
Query: 140 RRIATTELLSTKRLNYFSKLREEEVKLWVKRLYGSVTIREVQKGGRVGLRSKLTELSFNI 199
RRI + E+LS+ RLN F ++R +E + RL + + V LR +E++FN
Sbjct: 129 RRILSIEILSSHRLNSFLEIRRDE----IMRLIQKLAQKSYNGFTEVELRPMFSEMTFNT 188
Query: 200 ALMLMAGRRYFGEEVEEGESEEAKELKETMKQVPLLSGASYPADFLSVLKLIDFQGFRKR 259
+ +++G+RY+G + + + EEA+ + +K+V L GA+ DFL L+ DF G KR
Sbjct: 189 IMRMVSGKRYYGNDCDVSDVEEARLFRGIIKEVVSLGGANNVGDFLGFLRWFDFDGLEKR 248
Query: 260 LAKVRVDGDAFAQSLVDERRELKGSWCERKTMIDAMLSLQESDPEYYKDDIIKGQFLTLL 319
L K+ DAF Q L+DE R K + TMID +L+ Q+S PEYY D IIKG + +L
Sbjct: 249 LKKISKRTDAFLQGLIDEHRFGKRN---SNTMIDHLLTQQQSQPEYYTDQIIKGLMVVML 308
Query: 320 AAGTDTIASTLEWAMSLLLNHPIVMNKAWTEINQVVGDQRLVREADTNNLHYLQSIINET 379
AGTDT + T+EWAMS LLNHP +M KA E++ +G R V E D + L YLQSI+ ET
Sbjct: 309 LAGTDTSSVTIEWAMSNLLNHPEIMKKAKNELDTHIGHDRQVDEHDISKLPYLQSIVYET 368
Query: 380 YRLFPALPILVPRESSEDCTIGGFDIPKGTMLVVNAWAVHRNPEVWEDPNCFKPERF-KQ 439
RL A P+LVP SSED ++GG++IP+ T+L+VNAW +HR+P +W DP CFKPERF K+
Sbjct: 369 LRLHAAAPLLVPHLSSEDFSLGGYNIPQNTILMVNAWVIHRDPNLWSDPTCFKPERFEKE 428
Query: 440 GETVEVSKLLPFGMGRRACPGAGLAHRVVSLALATLIQCFEWGKLDDKKDVDLCPGIGLT 499
G EV+KLL FG+GRRACPG L+ R L L LIQCFEW ++ ++K +D+ G+T
Sbjct: 429 G---EVNKLLSFGLGRRACPGENLSQRTEGLTLGLLIQCFEWKRIGEEK-IDMVEAKGIT 488
Query: 500 MPKAIPLEAMCKPR 513
K L AMCK R
Sbjct: 489 AGKKTSLNAMCKVR 490
BLAST of Cmc07g0183611 vs. ExPASy Swiss-Prot
Match:
Q6WNR0 (Isoflavone 2'-hydroxylase OS=Medicago truncatula OX=3880 GN=CYP81E7 PE=1 SV=1)
HSP 1 Score: 435.3 bits (1118), Expect = 1.0e-120
Identity = 214/492 (43.50%), Postives = 324/492 (65.85%), Query Frame = 0
Query: 22 AIFLTTLFLISKFRRSEHTKLLLPPSPPSLPVIGHLHLLKEPFHQMLQDLSLKYGPVFSL 81
++F ++F I + LPP P SLP+IG+LH LK P ++ + L+ KYG V SL
Sbjct: 10 SLFYLSIFFIIRLLFQSRKFKNLPPGPTSLPIIGNLHHLKRPLNRTFKALTEKYGNVISL 69
Query: 82 TLGFRPLVVVSSPSAVHECFTKNDIVFASRPRMLSGKILNYDYTAVGATAYGQHWRIMRR 141
G R +VVVSS S ECFTKND+V A+RPR LSGK + Y+YT +G+T+YG+HWR +RR
Sbjct: 70 WFGSRLVVVVSSLSEFQECFTKNDVVLANRPRFLSGKYIFYNYTTLGSTSYGEHWRNLRR 129
Query: 142 IATTELLSTKRLNYFSKLREEEVKLWVKRLYGSVTIREVQKGGRVGLRSKLTELSFNIAL 201
I + ++LS R+N F+ +R +E + +K+L + K V L + +++FN +
Sbjct: 130 ITSLDVLSNHRINNFAPIRRDETQRLIKKLAEDSS----TKFAEVELTFRFFDMTFNNIM 189
Query: 202 MLMAGRRYFGEEVEEGESEEAKELKETMKQVPLLSGASYPADFLSVLKLIDFQGFRKRLA 261
+++G+RY+G++ + E +EA + ++ + ++ LSGA+ DF+ +LK +DF+ KR+
Sbjct: 190 RMISGKRYYGDDCDISEVQEASQFRDMVSELLQLSGANNKTDFMPLLKFLDFENLEKRVK 249
Query: 262 KVRVDGDAFAQSLVDERRELKGSWCERKTMIDAMLSLQESDPEYYKDDIIKGQFLTLLAA 321
++ D F L+ E+R K TMID +L++QES PEYY D IIKG L +L A
Sbjct: 250 RIGEKNDVFLSGLLQEQRSKKE---RTNTMIDHLLNMQESQPEYYTDTIIKGLCLAMLLA 309
Query: 322 GTDTIASTLEWAMSLLLNHPIVMNKAWTEINQVVGDQRLVREADTNNLHYLQSIINETYR 381
GTD+ A TLEW MS +LN+P V+ K E++ VG RLV E+D L YL+++I ET R
Sbjct: 310 GTDSSAVTLEWTMSNILNYPEVLKKVRDEVDTHVGQDRLVDESDLPKLTYLRNVIYETLR 369
Query: 382 LFPALPILVPRESSEDCTIGGFDIPKGTMLVVNAWAVHRNPEVWEDPNCFKPERF-KQGE 441
L+ P+L+P ++++C +GG+ +P+ T++++NAWA+HR+PE W + FKPERF K+G
Sbjct: 370 LYTPAPLLLPHSTADECIMGGYKVPRDTIVLINAWAIHRDPETWSEATTFKPERFDKKG- 429
Query: 442 TVEVSKLLPFGMGRRACPGAGLAHRVVSLALATLIQCFEWGKLDDKKDVDLCPGIGLTMP 501
E+ K++ FGMGRRACPG GLA R +S+ LA L+QCF+W +++D+K +D+ G TM
Sbjct: 430 --ELEKMIAFGMGRRACPGEGLALRAISMTLALLVQCFDWKRINDEK-IDMSERDGFTMT 489
Query: 502 KAIPLEAMCKPR 513
K +PL+AMCK R
Sbjct: 490 KLLPLKAMCKTR 490
BLAST of Cmc07g0183611 vs. ExPASy TrEMBL
Match:
A0A5D3D9B5 (Isoflavone 2'-hydroxylase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold134G002940 PE=3 SV=1)
HSP 1 Score: 1039.6 bits (2687), Expect = 4.3e-300
Identity = 518/522 (99.23%), Postives = 520/522 (99.62%), Query Frame = 0
Query: 1 MDMEQQTSFFFFFSHFNFPLLAIFLTTLFLISKFRRSEHTKLLLPPSPPSLPVIGHLHLL 60
MDMEQQTSFFFFFSHFNFPLLAIFLTTLFLISKFRRSEHTKLLLPPSPPSLPVIGHLHLL
Sbjct: 1 MDMEQQTSFFFFFSHFNFPLLAIFLTTLFLISKFRRSEHTKLLLPPSPPSLPVIGHLHLL 60
Query: 61 KEPFHQMLQDLSLKYGPVFSLTLGFRPLVVVSSPSAVHECFTKNDIVFASRPRMLSGKIL 120
KEPFHQMLQDLSLKYGPVFSLTLGFRPLVVVSSPSAVHECFTKNDIVFASRPRMLSGKIL
Sbjct: 61 KEPFHQMLQDLSLKYGPVFSLTLGFRPLVVVSSPSAVHECFTKNDIVFASRPRMLSGKIL 120
Query: 121 NYDYTAVGATAYGQHWRIMRRIATTELLSTKRLNYFSKLREEEVKLWVKRLYGSVTIREV 180
NYDYTAVGATAYGQHWRIMRRIATTELLSTKRLN FSKLREEEVKLWVKRLYGSVTIREV
Sbjct: 121 NYDYTAVGATAYGQHWRIMRRIATTELLSTKRLNDFSKLREEEVKLWVKRLYGSVTIREV 180
Query: 181 QKGGRVGLRSKLTELSFNIALMLMAGRRYFGEEVEEGESEEAKELKETMKQVPLLSGASY 240
QKGGRVGLRSKLTELSFNIALML AGRRYFGEEVEEGESEEAKELKETMKQVPLLSGASY
Sbjct: 181 QKGGRVGLRSKLTELSFNIALMLTAGRRYFGEEVEEGESEEAKELKETMKQVPLLSGASY 240
Query: 241 PADFLSVLKLIDFQGFRKRLAKVRVDGDAFAQSLVDERRELKGSWCERKTMIDAMLSLQE 300
PADFLSVLKLIDFQGFRKRLAKVRVDGDAFAQSLVDERRELKGSWCERKTMIDAMLSLQE
Sbjct: 241 PADFLSVLKLIDFQGFRKRLAKVRVDGDAFAQSLVDERRELKGSWCERKTMIDAMLSLQE 300
Query: 301 SDPEYYKDDIIKGQFLTLLAAGTDTIASTLEWAMSLLLNHPIVMNKAWTEINQVVGDQRL 360
SDPEYYKDDIIKGQFLTLLAAGTDTIASTLEWAMSLLLNHPIVMNKAWTEINQVVGDQRL
Sbjct: 301 SDPEYYKDDIIKGQFLTLLAAGTDTIASTLEWAMSLLLNHPIVMNKAWTEINQVVGDQRL 360
Query: 361 VREADTNNLHYLQSIINETYRLFPALPILVPRESSEDCTIGGFDIPKGTMLVVNAWAVHR 420
VREADTNNLHYLQSIINETYRLFPALPILVPRESSEDCTIGGFDIPKGTMLVVNAWAVHR
Sbjct: 361 VREADTNNLHYLQSIINETYRLFPALPILVPRESSEDCTIGGFDIPKGTMLVVNAWAVHR 420
Query: 421 NPEVWEDPNCFKPERFKQGETVEVSKLLPFGMGRRACPGAGLAHRVVSLALATLIQCFEW 480
NPEVWEDPNCFKPERFKQGETVEVSKLLPFGMGRRACPGAGLAHRVVSLALATLIQCFEW
Sbjct: 421 NPEVWEDPNCFKPERFKQGETVEVSKLLPFGMGRRACPGAGLAHRVVSLALATLIQCFEW 480
Query: 481 GKLDDKKDVDLCPGIGLTMPKAIPLEAMCKPRASITHLLSQL 523
GKLD+KKDVDLCPGIGLTMPKAIPLEAMCKPRAS+THLLSQL
Sbjct: 481 GKLDNKKDVDLCPGIGLTMPKAIPLEAMCKPRASVTHLLSQL 522
BLAST of Cmc07g0183611 vs. ExPASy TrEMBL
Match:
A0A1S3BTN1 (isoflavone 2'-hydroxylase-like OS=Cucumis melo OX=3656 GN=LOC103493583 PE=3 SV=1)
HSP 1 Score: 1039.6 bits (2687), Expect = 4.3e-300
Identity = 518/522 (99.23%), Postives = 520/522 (99.62%), Query Frame = 0
Query: 1 MDMEQQTSFFFFFSHFNFPLLAIFLTTLFLISKFRRSEHTKLLLPPSPPSLPVIGHLHLL 60
MDMEQQTSFFFFFSHFNFPLLAIFLTTLFLISKFRRSEHTKLLLPPSPPSLPVIGHLHLL
Sbjct: 1 MDMEQQTSFFFFFSHFNFPLLAIFLTTLFLISKFRRSEHTKLLLPPSPPSLPVIGHLHLL 60
Query: 61 KEPFHQMLQDLSLKYGPVFSLTLGFRPLVVVSSPSAVHECFTKNDIVFASRPRMLSGKIL 120
KEPFHQMLQDLSLKYGPVFSLTLGFRPLVVVSSPSAVHECFTKNDIVFASRPRMLSGKIL
Sbjct: 61 KEPFHQMLQDLSLKYGPVFSLTLGFRPLVVVSSPSAVHECFTKNDIVFASRPRMLSGKIL 120
Query: 121 NYDYTAVGATAYGQHWRIMRRIATTELLSTKRLNYFSKLREEEVKLWVKRLYGSVTIREV 180
NYDYTAVGATAYGQHWRIMRRIATTELLSTKRLN FSKLREEEVKLWVKRLYGSVTIREV
Sbjct: 121 NYDYTAVGATAYGQHWRIMRRIATTELLSTKRLNDFSKLREEEVKLWVKRLYGSVTIREV 180
Query: 181 QKGGRVGLRSKLTELSFNIALMLMAGRRYFGEEVEEGESEEAKELKETMKQVPLLSGASY 240
QKGGRVGLRSKLTELSFNIALML AGRRYFGEEVEEGESEEAKELKETMKQVPLLSGASY
Sbjct: 181 QKGGRVGLRSKLTELSFNIALMLTAGRRYFGEEVEEGESEEAKELKETMKQVPLLSGASY 240
Query: 241 PADFLSVLKLIDFQGFRKRLAKVRVDGDAFAQSLVDERRELKGSWCERKTMIDAMLSLQE 300
PADFLSVLKLIDFQGFRKRLAKVRVDGDAFAQSLVDERRELKGSWCERKTMIDAMLSLQE
Sbjct: 241 PADFLSVLKLIDFQGFRKRLAKVRVDGDAFAQSLVDERRELKGSWCERKTMIDAMLSLQE 300
Query: 301 SDPEYYKDDIIKGQFLTLLAAGTDTIASTLEWAMSLLLNHPIVMNKAWTEINQVVGDQRL 360
SDPEYYKDDIIKGQFLTLLAAGTDTIASTLEWAMSLLLNHPIVMNKAWTEINQVVGDQRL
Sbjct: 301 SDPEYYKDDIIKGQFLTLLAAGTDTIASTLEWAMSLLLNHPIVMNKAWTEINQVVGDQRL 360
Query: 361 VREADTNNLHYLQSIINETYRLFPALPILVPRESSEDCTIGGFDIPKGTMLVVNAWAVHR 420
VREADTNNLHYLQSIINETYRLFPALPILVPRESSEDCTIGGFDIPKGTMLVVNAWAVHR
Sbjct: 361 VREADTNNLHYLQSIINETYRLFPALPILVPRESSEDCTIGGFDIPKGTMLVVNAWAVHR 420
Query: 421 NPEVWEDPNCFKPERFKQGETVEVSKLLPFGMGRRACPGAGLAHRVVSLALATLIQCFEW 480
NPEVWEDPNCFKPERFKQGETVEVSKLLPFGMGRRACPGAGLAHRVVSLALATLIQCFEW
Sbjct: 421 NPEVWEDPNCFKPERFKQGETVEVSKLLPFGMGRRACPGAGLAHRVVSLALATLIQCFEW 480
Query: 481 GKLDDKKDVDLCPGIGLTMPKAIPLEAMCKPRASITHLLSQL 523
GKLD+KKDVDLCPGIGLTMPKAIPLEAMCKPRAS+THLLSQL
Sbjct: 481 GKLDNKKDVDLCPGIGLTMPKAIPLEAMCKPRASVTHLLSQL 522
BLAST of Cmc07g0183611 vs. ExPASy TrEMBL
Match:
A0A0A0L2I1 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G641760 PE=3 SV=1)
HSP 1 Score: 963.8 bits (2490), Expect = 3.0e-277
Identity = 482/524 (91.98%), Postives = 499/524 (95.23%), Query Frame = 0
Query: 3 MEQQTSFFFFFSH----FNFPLLAIFLTTLFLISKFRRSEHTKLLLPPSPPSLPVIGHLH 62
MEQQTSFFF H + PLLAIFLT+LFLISKFRR++HTK LLPPSPPSLPVIGHLH
Sbjct: 1 MEQQTSFFFSQDHHFHPISIPLLAIFLTSLFLISKFRRTQHTK-LLPPSPPSLPVIGHLH 60
Query: 63 LLKEPFHQMLQDLSLKYGPVFSLTLGFRPLVVVSSPSAVHECFTKNDIVFASRPRMLSGK 122
LLKEPFHQMLQD S KYGPVFSLTLGFRPLVVVSSPSAV ECFTKNDIVFASRPRMLSGK
Sbjct: 61 LLKEPFHQMLQDFSKKYGPVFSLTLGFRPLVVVSSPSAVQECFTKNDIVFASRPRMLSGK 120
Query: 123 ILNYDYTAVGATAYGQHWRIMRRIATTELLSTKRLNYFSKLREEEVKLWVKRLYGSVTIR 182
ILNYDYTAVGATAYGQHWRIMRRIATTELLSTKRLN SK+REEEVKLWVKRLYGSVT
Sbjct: 121 ILNYDYTAVGATAYGQHWRIMRRIATTELLSTKRLNDLSKIREEEVKLWVKRLYGSVTRA 180
Query: 183 EVQKGGRVGLRSKLTELSFNIALMLMAGRRYFGEEVEEGESEEAKELKETMKQVPLLSGA 242
EV KGGRVGLRSKLTELSFNIALMLMAGRRYFG+E+E GESEEAKELKETMKQVPLLSGA
Sbjct: 181 EVNKGGRVGLRSKLTELSFNIALMLMAGRRYFGDEMEAGESEEAKELKETMKQVPLLSGA 240
Query: 243 SYPADFLSVLKLIDFQGFRKRLAKVRVDGDAFAQSLVDERRELKGSWCERKTMIDAMLSL 302
SYPADFLSVLKLIDFQGFRKRLAKVRVDGDAFAQSLVDERR+LKGSWCERKTMID+ML L
Sbjct: 241 SYPADFLSVLKLIDFQGFRKRLAKVRVDGDAFAQSLVDERRKLKGSWCERKTMIDSMLCL 300
Query: 303 QESDPEYYKDDIIKGQFLTLLAAGTDTIASTLEWAMSLLLNHPIVMNKAWTEINQVVGDQ 362
QESDPEYYKDDIIKGQFLTLLAAGTDT ASTLEWAMSLLLNHP V+ KAWTEINQVVGDQ
Sbjct: 301 QESDPEYYKDDIIKGQFLTLLAAGTDTTASTLEWAMSLLLNHPTVLEKAWTEINQVVGDQ 360
Query: 363 RLVREADTNNLHYLQSIINETYRLFPALPILVPRESSEDCTIGGFDIPKGTMLVVNAWAV 422
RL+READTN LHYLQ+IINETYRLFPALPILVPRESSEDCTIGGFDIPKGTMLVVNAWAV
Sbjct: 361 RLIREADTNKLHYLQAIINETYRLFPALPILVPRESSEDCTIGGFDIPKGTMLVVNAWAV 420
Query: 423 HRNPEVWEDPNCFKPERFKQGETVEVSKLLPFGMGRRACPGAGLAHRVVSLALATLIQCF 482
HR+P VWEDPNCFKPERFKQGETVEV+KLLPFGMGRRACPGAGLAHRVVSLALATLIQCF
Sbjct: 421 HRDPNVWEDPNCFKPERFKQGETVEVNKLLPFGMGRRACPGAGLAHRVVSLALATLIQCF 480
Query: 483 EWGKLDDKKDVDLCPGIGLTMPKAIPLEAMCKPRASITHLLSQL 523
EWGKLD++KDVDLCPGIGLTMPKAIPLEAMCKPRAS+THLLSQL
Sbjct: 481 EWGKLDNQKDVDLCPGIGLTMPKAIPLEAMCKPRASVTHLLSQL 523
BLAST of Cmc07g0183611 vs. ExPASy TrEMBL
Match:
A0A6J1FJ78 (cytochrome P450 81E8-like OS=Cucurbita moschata OX=3662 GN=LOC111445861 PE=3 SV=1)
HSP 1 Score: 794.7 bits (2051), Expect = 2.4e-226
Identity = 400/510 (78.43%), Postives = 442/510 (86.67%), Query Frame = 0
Query: 13 FSHFNFPLLAIFLTTLFLISKFRRSEHTKLLLPPSPPSLPVIGHLHLLKEPFHQMLQDLS 72
FS+F PLL + L L LI KFR++EH LPP PPSLPVIGHLHLLKEPFHQMLQDLS
Sbjct: 11 FSNFEVPLLVLIL-ALLLIFKFRKAEHKN--LPPGPPSLPVIGHLHLLKEPFHQMLQDLS 70
Query: 73 LKYGPVFSLTLGFRPLVVVSSPSAVHECFTKNDIVFASRPRMLSGKILNYDYTAVGATAY 132
KYGP+ SL+LGFRP+VVVSSP+A ECF+KNDI+FASRPRM+SGKILNYDYTAVGATAY
Sbjct: 71 QKYGPILSLSLGFRPVVVVSSPAAAQECFSKNDIIFASRPRMISGKILNYDYTAVGATAY 130
Query: 133 GQHWRIMRRIATTELLSTKRLNYFSKLREEEVKLWVKRLYGSVTIREVQKGGRVGLRSKL 192
GQHWRIMRRIATTELLST RLN +R++EVKLW+K LY V RV +RSKL
Sbjct: 131 GQHWRIMRRIATTELLSTYRLNALINIRQQEVKLWLKSLYRISGKNNV----RVEIRSKL 190
Query: 193 TELSFNIALMLMAGRRYFGEEVEEGESEEAKELKETMKQVPLLSGASYPADFLSVLKLID 252
TELSFNIALMLM G+RYFG+E + +SEEA+ELK TMKQVPLLSGASYPADFLSVLKL+D
Sbjct: 191 TELSFNIALMLMVGKRYFGDEAVD-DSEEARELKNTMKQVPLLSGASYPADFLSVLKLVD 250
Query: 253 FQGFRKRLAKVRVDGDAFAQSLVDERRELKGSWCERKTMIDAMLSLQESDPEYYKDDIIK 312
+QGFRKRLAKVRVDGDAFAQSL+DERR K ERKTMID++LS+Q+ +PEYYKDDIIK
Sbjct: 251 YQGFRKRLAKVRVDGDAFAQSLIDERRNKKADDNERKTMIDSLLSMQQMEPEYYKDDIIK 310
Query: 313 GQFLTLLAAGTDTIASTLEWAMSLLLNHPIVMNKAWTEINQVVGDQRLVREADTNNLHYL 372
GQFLTLLAAGTDT A TLEWA+SLLLNHP M KAWTEIN+ VGD+RLV E D N LHYL
Sbjct: 311 GQFLTLLAAGTDTTAGTLEWALSLLLNHPTAMKKAWTEINEHVGDERLVEEMDLNQLHYL 370
Query: 373 QSIINETYRLFPALPILVPRESSEDCTIGGFDIPKGTMLVVNAWAVHRNPEVWEDPNCFK 432
Q IINETYRLFPALPILVPRESSEDCTIGGFD+PKGTMLVVNAWA+HR+P+VWEDPN F+
Sbjct: 371 QGIINETYRLFPALPILVPRESSEDCTIGGFDVPKGTMLVVNAWAIHRDPKVWEDPNSFR 430
Query: 433 PERFKQGETVEVSKLLPFGMGRRACPGAGLAHRVVSLALATLIQCFEWGKLDDKKDVDLC 492
PERFK+GE+VEVSKLLPFGMGRRACPGAGLAHRVV LALATLIQCFEW +LD+ K+VDL
Sbjct: 431 PERFKEGESVEVSKLLPFGMGRRACPGAGLAHRVVGLALATLIQCFEWERLDN-KEVDLT 490
Query: 493 PGIGLTMPKAIPLEAMCKPRASITHLLSQL 523
PGIGLTMPKA PL AM KPRA I HLLSQL
Sbjct: 491 PGIGLTMPKARPLVAMFKPRAPIIHLLSQL 511
BLAST of Cmc07g0183611 vs. ExPASy TrEMBL
Match:
A0A6J1J4B3 (isoflavone 3'-hydroxylase-like OS=Cucurbita maxima OX=3661 GN=LOC111481120 PE=3 SV=1)
HSP 1 Score: 785.0 bits (2026), Expect = 1.9e-223
Identity = 394/510 (77.25%), Postives = 440/510 (86.27%), Query Frame = 0
Query: 13 FSHFNFPLLAIFLTTLFLISKFRRSEHTKLLLPPSPPSLPVIGHLHLLKEPFHQMLQDLS 72
FS+F LL I + L +I KFR+++H LPP PPSLPVIGHLHLLKEPFHQMLQDLS
Sbjct: 12 FSNFEV-LLLILVLALLMIFKFRKADHKN--LPPGPPSLPVIGHLHLLKEPFHQMLQDLS 71
Query: 73 LKYGPVFSLTLGFRPLVVVSSPSAVHECFTKNDIVFASRPRMLSGKILNYDYTAVGATAY 132
KYGP+ SL+LGFRP+VVVSSP+A ECF+KNDI+FASRPRM+SGKILNYDYTAVGATAY
Sbjct: 72 QKYGPILSLSLGFRPVVVVSSPAAAQECFSKNDIIFASRPRMISGKILNYDYTAVGATAY 131
Query: 133 GQHWRIMRRIATTELLSTKRLNYFSKLREEEVKLWVKRLYGSVTIREVQKGGRVGLRSKL 192
GQHWRIMRRIATTELLST RLN +R++EVKLW+K LY V RV +RSKL
Sbjct: 132 GQHWRIMRRIATTELLSTHRLNALINIRQQEVKLWLKSLYRITGKNNV----RVEIRSKL 191
Query: 193 TELSFNIALMLMAGRRYFGEEVEEGESEEAKELKETMKQVPLLSGASYPADFLSVLKLID 252
TELSFNIALMLM G+RYFG+E + +SEEA+ELK TMKQVPLLSGASYPADFLSVLKL+D
Sbjct: 192 TELSFNIALMLMVGKRYFGDEAVD-DSEEARELKNTMKQVPLLSGASYPADFLSVLKLVD 251
Query: 253 FQGFRKRLAKVRVDGDAFAQSLVDERRELKGSWCERKTMIDAMLSLQESDPEYYKDDIIK 312
+QGFRK+LAKVRVDGDAFAQSL+DERR K ERKTMID++LS+Q+ +PEYYKD IIK
Sbjct: 252 YQGFRKKLAKVRVDGDAFAQSLIDERRNKKADHNERKTMIDSLLSMQQMEPEYYKDHIIK 311
Query: 313 GQFLTLLAAGTDTIASTLEWAMSLLLNHPIVMNKAWTEINQVVGDQRLVREADTNNLHYL 372
GQFLTLLAAGTDT A TLEWA+SLLLNHP M KAWTEIN+ VGD+RLV E D N LHYL
Sbjct: 312 GQFLTLLAAGTDTTAGTLEWALSLLLNHPTAMKKAWTEINEHVGDERLVEEMDLNQLHYL 371
Query: 373 QSIINETYRLFPALPILVPRESSEDCTIGGFDIPKGTMLVVNAWAVHRNPEVWEDPNCFK 432
Q IINETYRLFPALPILVPRESSEDCTIGGFD+P GTMLVVNAWA+HR+P+VWEDPN F+
Sbjct: 372 QGIINETYRLFPALPILVPRESSEDCTIGGFDVPIGTMLVVNAWAIHRDPKVWEDPNSFR 431
Query: 433 PERFKQGETVEVSKLLPFGMGRRACPGAGLAHRVVSLALATLIQCFEWGKLDDKKDVDLC 492
PERFK+GE+VEVSKLLPFGMGRRACPGAGLAHRVV LALATLIQCFEW +LD+ K+VDL
Sbjct: 432 PERFKEGESVEVSKLLPFGMGRRACPGAGLAHRVVGLALATLIQCFEWERLDN-KEVDLT 491
Query: 493 PGIGLTMPKAIPLEAMCKPRASITHLLSQL 523
PGIGLTMPK+ PL AM KPRA ITHLLSQL
Sbjct: 492 PGIGLTMPKSSPLVAMFKPRAPITHLLSQL 512
BLAST of Cmc07g0183611 vs. TAIR 10
Match:
AT4G37370.1 (cytochrome P450, family 81, subfamily D, polypeptide 8 )
HSP 1 Score: 465.3 bits (1196), Expect = 6.4e-131
Identity = 244/508 (48.03%), Postives = 346/508 (68.11%), Query Frame = 0
Query: 18 FPLLAIFLTTLFLISKFRRSEHTKLLLPPSPP-SLPVIGHLHLLKEPFHQMLQDL--SLK 77
F +L + L+ ++LI K +R + LPPSP SLPVIGHL LLK P H+ L SL
Sbjct: 8 FSILFVVLSLIYLIGKLKRKPN----LPPSPAWSLPVIGHLRLLKPPIHRTFLSLSQSLN 67
Query: 78 YGPVFSLTLGFRPLVVVSSPSAVHECFTKNDIVFASRPRMLSGKILNYDYTAVGATAYGQ 137
P+FSL LG R + V SS S ECFTKND+V A+RP + K + YDYT + A +YG
Sbjct: 68 NAPIFSLRLGNRLVFVNSSHSIAEECFTKNDVVLANRPNFILAKHVAYDYTTMIAASYGD 127
Query: 138 HWRIMRRIATTELLSTKRLNYFSKLREEEVKLWVKRLYGSVTIREVQKGGRVGLRSKLTE 197
HWR +RRI + E+ S RLN F +R++E++ V RL + + Q+ +V ++S L++
Sbjct: 128 HWRNLRRIGSVEIFSNHRLNSFLSIRKDEIRRLVFRLSRNFS----QEFVKVDMKSMLSD 187
Query: 198 LSFNIALMLMAGRRYFGEEVEEGESEEAKELKETMKQVPLLSGASYPADFLSVLKLIDFQ 257
L+FN L ++AG+RY+G+ VE + EAK +++ + V +GA D+L VL+L+
Sbjct: 188 LTFNNILRMVAGKRYYGDGVE--DDPEAKRVRQLIADVVACAGAGNAVDYLPVLRLV--S 247
Query: 258 GFRKRLAKVRVDGDAFAQSLVDERRELKGSWCERKTMIDAMLSLQESDPEYYKDDIIKGQ 317
+ R+ K+ D F Q LVDE+RE K + TMID +L+LQES P+Y+ D IIKG
Sbjct: 248 DYETRVKKLAGRLDEFLQGLVDEKREAKE---KGNTMIDHLLTLQESQPDYFTDRIIKGN 307
Query: 318 FLTLLAAGTDTIASTLEWAMSLLLNHPIVMNKAWTEINQVVGDQRLVREADTNNLHYLQS 377
L L+ AGTDT A TLEWA+S +LNHP V+NKA EI++ +G RL+ E+D +NL YLQ+
Sbjct: 308 MLALILAGTDTSAVTLEWALSNVLNHPDVLNKARDEIDRKIGLDRLMDESDISNLPYLQN 367
Query: 378 IINETYRLFPALPILVPRESSEDCTIGGFDIPKGTMLVVNAWAVHRNPEVWEDPNCFKPE 437
I++ET RL+PA P+L+P +SEDC + G+D+P+GT+L+ N WA+HR+P++W+DP FKPE
Sbjct: 368 IVSETLRLYPAAPMLLPHVASEDCKVAGYDMPRGTILLTNVWAIHRDPQLWDDPMSFKPE 427
Query: 438 RF-KQGETVEVSKLLPFGMGRRACPGAGLAHRVVSLALATLIQCFEWGKLDDKKDVDLCP 497
RF K+G E KL+PFG+GRRACPG+GLAHR+++L L +LIQC EW K+ + +VD+
Sbjct: 428 RFEKEG---EAQKLMPFGLGRRACPGSGLAHRLINLTLGSLIQCLEWEKIGE--EVDMSE 487
Query: 498 GIGLTMPKAIPLEAMCKPRASITHLLSQ 522
G G+TMPKA PLEAMC+ R S+ + ++
Sbjct: 488 GKGVTMPKAKPLEAMCRARPSVVKIFNE 495
BLAST of Cmc07g0183611 vs. TAIR 10
Match:
AT4G37330.1 (cytochrome P450, family 81, subfamily D, polypeptide 4 )
HSP 1 Score: 456.4 bits (1173), Expect = 3.0e-128
Identity = 246/503 (48.91%), Postives = 337/503 (67.00%), Query Frame = 0
Query: 21 LAIFLTTLFLISKFRRSEHTKLLLPPSPP-SLPVIGHLHLLKEPFHQMLQDLSLKYG--P 80
L++F +FL+++ +R KL LPPSP SLPVIGHLHLLK P H+ LS G P
Sbjct: 12 LSLFTLAIFLLTRSKR----KLNLPPSPAISLPVIGHLHLLKPPLHRTFLSLSKSIGNAP 71
Query: 81 VFSLTLGFRPLVVVSSPSAVHECFTKNDIVFASRPRMLSGKILNYDYTAVGATAYGQHWR 140
VF L LG R + V+SS S ECFTKND+V A+RP+ K L Y+ T + + +YG HWR
Sbjct: 72 VFHLRLGNRLVYVISSRSIAEECFTKNDVVLANRPKFTISKHLGYNATYLLSASYGDHWR 131
Query: 141 IMRRIATTELLSTKRLNYFSKLREEEVKLWVKRLYGSVTIREVQKGGRVGLRSKLTELSF 200
+RRIA E+ ST RLN F +R++E ++RL ++ + V +++ LT L+
Sbjct: 132 NLRRIAAVEIFSTHRLNSFLYIRKDE----IRRLISHLSRDSLHGFVEVEMKTLLTNLAS 191
Query: 201 NIALMLMAGRRYFGEEVEEGESEEAKELKETMKQVPLLSGASYPADFLSVLKLIDFQGFR 260
N + ++AG+RYFGE ++++AK +K + + +GA P D+LS+L+ + +
Sbjct: 192 NTTIRMLAGKRYFGE-----DNDDAKLVKNLVSEAVTSAGAGNPIDYLSILRWV--SSYE 251
Query: 261 KRLAKVRVDGDAFAQSLVDERRELKGSWCERKTMIDAMLSLQESDPEYYKDDIIKGQFLT 320
KR+ + D F Q LVDE+R K + +TMID +L+LQ+ P+YY D IIKG LT
Sbjct: 252 KRIKNLGNRFDTFLQKLVDEKRAEKE---KGETMIDHLLALQDIQPDYYTDVIIKGIILT 311
Query: 321 LLAAGTDTIASTLEWAMSLLLNHPIVMNKAWTEINQVVGDQRLVREADTNNLHYLQSIIN 380
L+ AGTDT + TLEWAMS LLNHP ++ KA EI++ VG RLV E+D NL YLQSI+
Sbjct: 312 LIIAGTDTSSVTLEWAMSNLLNHPEILKKARMEIDEKVGLDRLVDESDIVNLSYLQSIVL 371
Query: 381 ETYRLFPALPILVPRESSEDCTIGGFDIPKGTMLVVNAWAVHRNPEVWEDPNCFKPERF- 440
ET R++PA+P+L+P SSEDC +GG+DIP GTM++ NAWA+HR+PEVWEDP FKPERF
Sbjct: 372 ETLRMYPAVPLLLPHLSSEDCKVGGYDIPSGTMVLTNAWAMHRDPEVWEDPEIFKPERFE 431
Query: 441 KQGETVEVSKLLPFGMGRRACPGAGLAHRVVSLALATLIQCFEWGKLDDKKDVDLCPGIG 500
K+G E KL+ FGMGRRACPGAGLAHR+++ AL +L+QCFEW ++ + VD+ G
Sbjct: 432 KEG---EAEKLISFGMGRRACPGAGLAHRLINQALGSLVQCFEWERVGE-DFVDMTEDKG 491
Query: 501 LTMPKAIPLEAMCKPRASITHLL 520
T+PKAIPL AMCK R+ + L+
Sbjct: 492 ATLPKAIPLRAMCKARSIVDKLI 492
BLAST of Cmc07g0183611 vs. TAIR 10
Match:
AT4G37360.1 (cytochrome P450, family 81, subfamily D, polypeptide 2 )
HSP 1 Score: 443.4 bits (1139), Expect = 2.6e-124
Identity = 237/506 (46.84%), Postives = 336/506 (66.40%), Query Frame = 0
Query: 18 FPLLAIFLTTLFLISKFRRSEHTKLLLPPSPP-SLPVIGHLHLLKEPFHQMLQDLSLKYG 77
F I L+ +FLI + +R KL LPPSP +LPVIGHL LLK P H++ +S G
Sbjct: 8 FTFCFIVLSLIFLIGRIKR----KLNLPPSPAWALPVIGHLRLLKPPLHRVFLSVSQSLG 67
Query: 78 --PVFSLTLGFRPLVVVSSPSAVHECFTKNDIVFASRPRMLSGKILNYDYTAVGATAYGQ 137
P+ SL LG R L VVSS S ECFTKND++ A+R +S K ++Y + V + +Y +
Sbjct: 68 DAPIISLRLGNRLLFVVSSHSIAEECFTKNDVILANRQTTISTKHISYGNSTVVSASYSE 127
Query: 138 HWRIMRRIATTELLSTKRLNYFSKLREEEVKLWVKRLYGSVTIREVQKGGRVGLRSKLTE 197
HWR +RRI E+ S RLN FS +R +E ++RL G + +V ++S ++
Sbjct: 128 HWRNLRRIGALEIFSAHRLNSFSSIRRDE----IRRLIGRLLRNSSYGFTKVEMKSMFSD 187
Query: 198 LSFNIALMLMAGRRYFGEEVEEGESEEAKELKETMKQVPLLSGASYPADFLSVLKLIDFQ 257
L+FN + ++AG+ Y+G+ E + EAK ++ + + SG AD++ +L I +
Sbjct: 188 LTFNNIIRMLAGKCYYGDGKE--DDPEAKRVRTLIAEAMSSSGPGNAADYIPILTWITYS 247
Query: 258 GFRKRLAKVRVDGDAFAQSLVDERRELKGSWCERKTMIDAMLSLQESDPEYYKDDIIKGQ 317
R+ K+ D F Q LVDE+RE G + TM+D +L LQE+ PEYY D IIKG
Sbjct: 248 --ETRIKKLAGRLDEFLQGLVDEKRE--GKEKKENTMVDHLLCLQETQPEYYMDRIIKGT 307
Query: 318 FLTLLAAGTDTIASTLEWAMSLLLNHPIVMNKAWTEINQVVGDQRLVREADTNNLHYLQS 377
L+L+A GTDT A TLEWA+S LLN+P V+NKA EI++++G RL+ E+D NL YLQ+
Sbjct: 308 MLSLIAGGTDTTAVTLEWALSSLLNNPEVLNKARDEIDRMIGVDRLLEESDIPNLPYLQN 367
Query: 378 IINETYRLFPALPILVPRESSEDCTIGGFDIPKGTMLVVNAWAVHRNPEVWEDPNCFKPE 437
I++ET RL+PA P+L+P +S+DC +GG+D+P+GTML+ NAWA+HR+P +W+DP FKPE
Sbjct: 368 IVSETLRLYPAAPMLLPHVASKDCKVGGYDMPRGTMLLTNAWAIHRDPLLWDDPTSFKPE 427
Query: 438 RF-KQGETVEVSKLLPFGMGRRACPGAGLAHRVVSLALATLIQCFEWGKLDDKKDVDLCP 497
RF K+G E KL+PFG+GRRACPG+GLA R+V+L+L +LIQCFEW ++ + ++VD+
Sbjct: 428 RFEKEG---EAKKLMPFGLGRRACPGSGLAQRLVTLSLGSLIQCFEWERIGE-EEVDMTE 487
Query: 498 GIGLTMPKAIPLEAMCKPRASITHLL 520
G GLTMPKA PLEAMC+ R + +L
Sbjct: 488 GPGLTMPKARPLEAMCRARDFVGKIL 495
BLAST of Cmc07g0183611 vs. TAIR 10
Match:
AT5G67310.1 (cytochrome P450, family 81, subfamily G, polypeptide 1 )
HSP 1 Score: 436.4 bits (1121), Expect = 3.2e-122
Identity = 240/495 (48.48%), Postives = 317/495 (64.04%), Query Frame = 0
Query: 20 LLAIFLTTLFLISKFRRSEHTKLLLPPSPPSLPVIGHLHLLKEPFHQMLQDLSLKYG-PV 79
LLA+ + + FR + K LPP+P PVIGHLHLLKEP H+ L+DLS G V
Sbjct: 20 LLALMAGFIAVAYVFRSKQ--KKNLPPNPVGFPVIGHLHLLKEPVHRSLRDLSRNLGIDV 79
Query: 80 FSLTLGFRPLVVVSSPSAVHECFT-KNDIVFASRPRMLSGKILNYDYTAVGATAYGQHWR 139
F L LG R VVV+S SA E + +ND+VFA+RP + + Y+ T V YG+HWR
Sbjct: 80 FILRLGSRRAVVVTSASAAEEFLSQQNDVVFANRPLATLTEYMGYNNTLVSTAPYGEHWR 139
Query: 140 IMRRIATTELLSTKRLNYFSKLREEEVKLWVKRLYGSVTIREVQKGGRVGLRSK--LTEL 199
+RR ++LST RL FS +R +EV+ +++ + + V GG V L+ + L L
Sbjct: 140 RLRRFCAVDILSTARLRDFSDIRRDEVRAMIRK----INVELVTSGGSVRLKLQPFLYGL 199
Query: 200 SFNIALMLMAGRRYFGEEVEEGESEEAKELKETMKQVPLLSGASYPADFLSVLKLIDFQG 259
++NI + ++AG+R E EE KE+++ +++V +G +Y DFL LKL D G
Sbjct: 200 TYNILMSMVAGKR--------EEDEETKEVRKLIREVFDFAGVNYVGDFLPTLKLFDLDG 259
Query: 260 FRKRLAKVRVDGDAFAQSLVDERRELKGSWCERKTMIDAMLSLQESDPEYYKDDIIKGQF 319
+RKR K+ D F Q LVDE R+ +G KTMI +LSLQES+PE Y DDIIKG
Sbjct: 260 YRKRAKKLASKLDKFMQKLVDEHRKNRGKAELEKTMITRLLSLQESEPECYTDDIIKGLV 319
Query: 320 LTLLAAGTDTIASTLEWAMSLLLNHPIVMNKAWTEINQVVGDQRLVREADTNNLHYLQSI 379
+L AGTDT A TLEWAM+ LLNHP V+ K TE+N+V + R+ E+DT YL ++
Sbjct: 320 QVMLLAGTDTTAVTLEWAMANLLNHPEVLRKLKTELNEVSKEGRVFEESDTGKCPYLNNV 379
Query: 380 INETYRLFPALPILVPRESSEDCTIGGFDIPKGTMLVVNAWAVHRNPEVWEDPNCFKPER 439
I+ET RLFPA P+LVP SS DC + GFDIP+ T L +NAWA+ R+P VW+DP FKPER
Sbjct: 380 ISETLRLFPAAPLLVPHASSTDCEVAGFDIPRRTWLFINAWAIQRDPNVWDDPETFKPER 439
Query: 440 FKQGETVEVSKLLPFGMGRRACPGAGLAHRVVSLALATLIQCFEWGKLDDKKDVDLCPGI 499
F + ET K LPFG+GRRACPG GLA V+SLAL +LIQCF+W + D+ VD+ G
Sbjct: 440 F-ESET-HRGKFLPFGIGRRACPGMGLAQLVLSLALGSLIQCFDWER-DNDVAVDMSEGK 497
Query: 500 GLTMPKAIPLEAMCK 511
GLTMPK++PL A CK
Sbjct: 500 GLTMPKSVPLVAKCK 497
BLAST of Cmc07g0183611 vs. TAIR 10
Match:
AT4G37340.1 (cytochrome P450, family 81, subfamily D, polypeptide 3 )
HSP 1 Score: 434.5 bits (1116), Expect = 1.2e-121
Identity = 243/524 (46.37%), Postives = 337/524 (64.31%), Query Frame = 0
Query: 3 MEQQTSFFFFFSHFNFPLLAIFLTTLFLISKFRRSEHTKLLLPPSPP-SLPVIGHLHLLK 62
ME QT F F L I L+ F+I + +R + LPPSP +LPVIGHL LLK
Sbjct: 1 MEGQTLIFTF--------LFISLSLTFIIGRIKRRPN----LPPSPSWALPVIGHLRLLK 60
Query: 63 EPFHQMLQDLSLKYG--PVFSLTLGFRPLVVVSSPSAVHECFTKNDIVFASRPRMLSGKI 122
P H++ +S G P+ SL LG R + VVSS S ECFTKND+V A+R L+ K
Sbjct: 61 PPLHRVFLSVSESLGDAPIISLRLGNRLVFVVSSHSLAEECFTKNDVVLANRFNSLASKH 120
Query: 123 LNYDYTAVGATAYGQHWRIMRRIATTELLSTKRLNYFSKLREEEVKLWVKRLYGSVTIRE 182
++Y T V +YG HWR +RRI E+ S RLN FS +R +E + RL ++
Sbjct: 121 ISYGCTTVVTASYGDHWRNLRRIGAVEIFSAHRLNSFSSIRRDE----IHRLIACLSRNS 180
Query: 183 VQKGGRVGLRSKLTELSFNIALMLMAGRRYFGEEVEEGESEEAKELKETMKQVPLLSGAS 242
+ +V ++S + L+FN + ++AG+ Y+G+ E + EAK ++E + + GA
Sbjct: 181 SLEFTKVEMKSMFSNLTFNNIIRMLAGKCYYGDGAE--DDPEAKRVRELIAEGMGCFGAG 240
Query: 243 YPADFLSVLKLIDFQGFRKRLAKVRVDGDAFAQSLVDERRELKGSWCERKTMIDAMLSLQ 302
AD+L +L I G KR+ K+ D F Q LVDERRE G + TM+D +L LQ
Sbjct: 241 NTADYLPILTWI--TGSEKRIKKIASRLDEFLQGLVDERRE--GKEKRQNTMVDHLLCLQ 300
Query: 303 ESDPEYYKDDIIKGQFLTLLAAGTDTIASTLEWAMSLLLNHPIVMNKAWTEINQVVGDQR 362
E+ PEYY D+IIKG L+L+ AGTDT A TLEW +S LLNHP +++KA EI+ VG R
Sbjct: 301 ETQPEYYTDNIIKGIMLSLILAGTDTSAVTLEWTLSALLNHPQILSKARDEIDNKVGLNR 360
Query: 363 LVREADTNNLHYLQSIINETYRLFPALPILVPRESSEDCTIGGFDIPKGTMLVVNAWAVH 422
LV E+D ++L YLQ+I++E+ RL+PA P+LVP +SEDC +GG+ +P+GTML+ NAWA+H
Sbjct: 361 LVEESDLSHLPYLQNIVSESLRLYPASPLLVPHVASEDCKVGGYHMPRGTMLLTNAWAIH 420
Query: 423 RNPEVWEDPNCFKPERF-KQGETVEVSKLLPFGMGRRACPGAGLAHRVVSLALATLIQCF 482
R+P++W+DP FKPERF K+G E KLL FG+GRRACPG+GLA R+ SL + +LIQCF
Sbjct: 421 RDPKIWDDPTSFKPERFEKEG---EAQKLLGFGLGRRACPGSGLAQRLASLTIGSLIQCF 480
Query: 483 EWGKLDDKKDVDLCP-GIGLTMPKAIPLEAMCKPRASITHLLSQ 522
EW ++ + ++VD+ G G+ MPKAIPL AMCK R + +L++
Sbjct: 481 EWERIGE-EEVDMTEGGGGVIMPKAIPLVAMCKARPVVGKILNE 498
The following BLAST results are available for this feature:
Match Name | E-value | Identity | Description | |
XP_008452612.1 | 8.8e-300 | 99.23 | PREDICTED: isoflavone 2'-hydroxylase-like [Cucumis melo] >KAA0064425.1 isoflavon... | [more] |
XP_004141394.1 | 6.2e-277 | 91.98 | cytochrome P450 81Q32 [Cucumis sativus] >KGN55244.1 hypothetical protein Csa_012... | [more] |
XP_038900261.1 | 4.5e-227 | 80.04 | LOW QUALITY PROTEIN: cytochrome P450 81Q32-like [Benincasa hispida] | [more] |
XP_022940149.1 | 5.0e-226 | 78.43 | cytochrome P450 81E8-like [Cucurbita moschata] | [more] |
XP_023523499.1 | 4.2e-225 | 78.04 | cytochrome P450 81E8-like [Cucurbita pepo subsp. pepo] | [more] |
Match Name | E-value | Identity | Description | |
W8JMU7 | 7.9e-142 | 52.31 | Cytochrome P450 81Q32 OS=Catharanthus roseus OX=4058 GN=CYP81Q32 PE=2 SV=1 | [more] |
P93147 | 2.7e-126 | 45.92 | Isoflavone 2'-hydroxylase OS=Glycyrrhiza echinata OX=46348 GN=CYP81E1 PE=1 SV=2 | [more] |
Q6WNQ9 | 3.0e-125 | 45.98 | Isoflavone 3'-hydroxylase (Fragment) OS=Medicago truncatula OX=3880 GN=CYP81E9 P... | [more] |
Q6WNQ8 | 6.9e-122 | 46.76 | Cytochrome P450 81E8 OS=Medicago truncatula OX=3880 GN=CYP81E8 PE=2 SV=1 | [more] |
Q6WNR0 | 1.0e-120 | 43.50 | Isoflavone 2'-hydroxylase OS=Medicago truncatula OX=3880 GN=CYP81E7 PE=1 SV=1 | [more] |
Match Name | E-value | Identity | Description | |
A0A5D3D9B5 | 4.3e-300 | 99.23 | Isoflavone 2'-hydroxylase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_s... | [more] |
A0A1S3BTN1 | 4.3e-300 | 99.23 | isoflavone 2'-hydroxylase-like OS=Cucumis melo OX=3656 GN=LOC103493583 PE=3 SV=1 | [more] |
A0A0A0L2I1 | 3.0e-277 | 91.98 | Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G641760 PE=3 SV=1 | [more] |
A0A6J1FJ78 | 2.4e-226 | 78.43 | cytochrome P450 81E8-like OS=Cucurbita moschata OX=3662 GN=LOC111445861 PE=3 SV=... | [more] |
A0A6J1J4B3 | 1.9e-223 | 77.25 | isoflavone 3'-hydroxylase-like OS=Cucurbita maxima OX=3661 GN=LOC111481120 PE=3 ... | [more] |
Match Name | E-value | Identity | Description | |
AT4G37370.1 | 6.4e-131 | 48.03 | cytochrome P450, family 81, subfamily D, polypeptide 8 | [more] |
AT4G37330.1 | 3.0e-128 | 48.91 | cytochrome P450, family 81, subfamily D, polypeptide 4 | [more] |
AT4G37360.1 | 2.6e-124 | 46.84 | cytochrome P450, family 81, subfamily D, polypeptide 2 | [more] |
AT5G67310.1 | 3.2e-122 | 48.48 | cytochrome P450, family 81, subfamily G, polypeptide 1 | [more] |
AT4G37340.1 | 1.2e-121 | 46.37 | cytochrome P450, family 81, subfamily D, polypeptide 3 | [more] |