Cmc07g0183581 (gene) Melon (Charmono) v1.1

Overview
NameCmc07g0183581
Typegene
OrganismCucumis melo L. var. cantalupensis cv. Charmono (Melon (Charmono) v1.1)
Descriptioncytochrome P450 81E8-like
LocationCMiso1.1chr07: 1980361 .. 1983470 (-)
RNA-Seq ExpressionCmc07g0183581
SyntenyCmc07g0183581
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CTTCCCCCAACTTCTGTACCCATTATGCCCTACATCGCATCTCATTTTATTCTTCTCATTCTTCTTTTTACAAACCTCCATCTTATAATAATAACAATGCTCTATTGAATTTCCCATCCATTAAACTCAATTTTGTTTCATTTACAATTAATCACATCCTGACTACAATGCCACCACCACCCATCTTCAAATTAATGGAAAAATTAATTGCAAACGAAACAAAATTCAGTTTACGAATGGCGAATTCTCCGTCTCATTTCATTTTCTACATCAACGATTAGTGGATTTGAGAAAAAAAAAAGTTCCCCCTTTCCATTTTGCCCAGAAAATGACCATTCTGCCCTCACTGGGTTCTTTGATTTCTTTTTCTTTCAGGGTATTTAAGAAAAACCGCAAGGAATTTGTGTATGAGATTTTATTTTTGGCTCTTCTTTGCGACTTGAAGTTTTATGGGTTTTTGCTTTTGGTTTTGCTTCTCTCAACCAAGGAAGCTATCGTTTCTACTTCGGATGGAAGATGGTTCATGGATGGTAACGTTTTTCTTTTTTCTAATCTATTTAACATTTTAGTTTTGAAAATTTCAGTATTAATACTATTTTTGAGAATTTGTTATAGAGGAGATTCAGTTTATAATGTTTGAGAAATTTAAAAATAATGTAAGTATTGTATTCTTTGTTCATATATCCAAGTTTTTTTTTTCCACGATTCCCTTTCACTAAAGATTCATTTTATTAAATAATTGTTTAGTTTTTAAATAAGTATTAAACCTAATGTAATTAAATTTAAGAAAAATTATATTTTTAGTTTCAATAAATTTTTAAAAGGTACGTGGTAGTTCATGAGTTTGAAAATTTTACATTTGTAGTGTCCAGATTTTAAAGTTGATTCGTAGATTTGTTTCGTGTCTAAATTTTGAAAATATAAATTTTTTAATTGTTGAGTTTTTAAAAATAAGTTTAAAAATAATTATTTAATATTTTACATTAGAGATTAAAAATATACATTCAAACCTAAAGCTCAATGTAGTCGGTAAACGAAATGTTAATTACATAAATATAGTTTTTTTGTTTTATAATTTACAAATGATCAGGGTTATAGTTTACAAATGATTATCGCAGGTAAATTCCATGGTGATTTCATTCCTATATCTTCTAATTTTATCCATACTTCAAATCTTCACAAATCACTTCCTTCATAGAATCCGAAATCTTCCTCCAAGCCCATTTCTTTCTCTACCCATTATAGGCCAACTATACCTCCTTAAAGCCCCCCTCCACCGGACCCTATCGGAAATCTCCCGCCGTCATGGCCCAATCCTCTTCTTCCAATTCGGTTTCCGTCCAGTACTGGTCGTTTCATCTCCGTCGGCCGCCGAGGACTGCCTATCCAAAAACGACGTCATCTTCGCCAATCGACCTCGTCTTGTTAGCGGCAAATACCTCGGGTATAATTACACAAGCCTCCTATGGGCGCCATACGGAGAGCATTGGCGAAACCTCCGTCGAATCTCGTCGTTAGAAATCCTTTCCAGCCACCGCCTCCAAACGCTTTCAAGTATTCGCGTGGATGAAGTCCGTAAGCTGATCCGGAGACTGTATAATGCTGAAAACGACGTCGTAGACATGAGGACAGAGTTTTTCCAATTGATGTTCAATATCATGATGAGAATGATCGCCGGAAAACGATATTATGGGGAAGCTGTGGTAGCGGACTCGGAGGAGGGGAGGAGATTTCAAGAGATACAAGAGGAAACGTTTCGATTAAGCGGCAAGACTAATTTGGGGGACTTTTTAAGAGTTGTGAAATGGGTTGGATTATCTAAAGGGATTGAAAATAGATTGAGGGAGTTACAGATTAGGAGGGATGATTGGATGCAAAGTTTGATTGAAGAACATCGGAAGAAAATGGATAATGCTCATAGTTCTTCTTCTTCTTCTATTCAAGGTGATGCTAAAAAGACTATGATTGAGGTTCTCCTCTCATTGCAACAAATGGAACCTCAGTATTATAAAGACGAGTATATTAGAGGCCTCATGCTCGTGAGTTAACCTGATTTCTCTCTTAACTCAATTCAAAACTCACAATCAAATAGATTTTGAAATACTGTTTTAATTGGAATTTTAAAATTTGCTAGAAAATATGTAATCAAACTCTATTTTTCATTGAATCTACTAAATCGTCCCTAATAAAATTGGATCGTTGTAGGTTTTGCTATTAGCCGGAACAGAAGGTTCCATCAATACAATGGAGTGGCTTCTTTCACTATTACTCAATCACCCACACTCTCTTCGACGGGCCCAAATGGAAATCGACAACGTAGTTGGAAAAACTAATCGACTCCTGGAGGAATCGGATCTGACCCACCTTCCTTACCTCCGTAGCCTGATTCACGAGACTCTTCGAATGTACCCGCCAGGCCCATTATTGATTCCACACGAATCATCGCAGGATTGCCACGTTGGAGGGTTCCATGTCCCAGCTGGCACAATGTTATTCGTCAACGTATGGGCCATTCAAAACGACCCCACCGTATGGGTGGAGCCGGAGAAATTTAACCCAGATAGATTCAAAAGCGATGGAGAGGGGTTCAAATGGATGCCATTTGGGGCTGGGCGTAGGAGGTGTCCCGGCGAGAGCTTAGGACTTCGTGTCGTTGGATTGGTCATAGGATCGCTAATTCAGTGCTTTGAATGGGAGTCAATGGGCGGAAAATGTATTGATATGAGCGAAGGTGGTGGATTGACCTTGCCCAAAGCTCTCCCCCTCCGCACCCTTTGTCGGCCACGTTCAAATGCCACACATCTGCTTTCCCAAATCTGATTCAAAATGCATGGTCTTGTTTAGTTTGTACAACAACTTTTAATTCTGTACAGAAATTTGACATATTTTAGTAGTTGAAAACATCTACATTATTTTCAATTTGTTGACGTCCTATTTTCCCTTTTTCTTTTGCCTCTTTGTTTTTTGTAATGATTGGTCGATGTCCTCTATTTTAGTAGAAGCAAAAGTACTCTTCGTGAGATAATGTTTAAATTGAGTGAATGATGAAAGAAAGAGCATCATTTATCAATTGATACTATGCTCTTCTAAGTACGTAAAATGACAAAATT

mRNA sequence

CTTCCCCCAACTTCTGTACCCATTATGCCCTACATCGCATCTCATTTTATTCTTCTCATTCTTCTTTTTACAAACCTCCATCTTATAATAATAACAATGCTCTATTGAATTTCCCATCCATTAAACTCAATTTTGTTTCATTTACAATTAATCACATCCTGACTACAATGCCACCACCACCCATCTTCAAATTAATGGAAAAATTAATTGCAAACGAAACAAAATTCAGTTTACGAATGGCGAATTCTCCGTCTCATTTCATTTTCTACATCAACGATTAGTGGATTTGAGAAAAAAAAAAGTTCCCCCTTTCCATTTTGCCCAGAAAATGACCATTCTGCCCTCACTGGGTTCTTTGATTTCTTTTTCTTTCAGGGTATTTAAGAAAAACCGCAAGGAATTTGTGTATGAGATTTTATTTTTGGCTCTTCTTTGCGACTTGAAGTTTTATGGGTTTTTGCTTTTGGTTTTGCTTCTCTCAACCAAGGAAGCTATCGTTTCTACTTCGGATGGAAGATGGTTCATGGATGGTAAATTCCATGGTGATTTCATTCCTATATCTTCTAATTTTATCCATACTTCAAATCTTCACAAATCACTTCCTTCATAGAATCCGAAATCTTCCTCCAAGCCCATTTCTTTCTCTACCCATTATAGGCCAACTATACCTCCTTAAAGCCCCCCTCCACCGGACCCTATCGGAAATCTCCCGCCGTCATGGCCCAATCCTCTTCTTCCAATTCGGTTTCCGTCCAGTACTGGTCGTTTCATCTCCGTCGGCCGCCGAGGACTGCCTATCCAAAAACGACGTCATCTTCGCCAATCGACCTCGTCTTGTTAGCGGCAAATACCTCGGGTATAATTACACAAGCCTCCTATGGGCGCCATACGGAGAGCATTGGCGAAACCTCCGTCGAATCTCGTCGTTAGAAATCCTTTCCAGCCACCGCCTCCAAACGCTTTCAAGTATTCGCGTGGATGAAGTCCGTAAGCTGATCCGGAGACTGTATAATGCTGAAAACGACGTCGTAGACATGAGGACAGAGTTTTTCCAATTGATGTTCAATATCATGATGAGAATGATCGCCGGAAAACGATATTATGGGGAAGCTGTGGTAGCGGACTCGGAGGAGGGGAGGAGATTTCAAGAGATACAAGAGGAAACGTTTCGATTAAGCGGCAAGACTAATTTGGGGGACTTTTTAAGAGTTGTGAAATGGGTTGGATTATCTAAAGGGATTGAAAATAGATTGAGGGAGTTACAGATTAGGAGGGATGATTGGATGCAAAGTTTGATTGAAGAACATCGGAAGAAAATGGATAATGCTCATAGTTCTTCTTCTTCTTCTATTCAAGGTGATGCTAAAAAGACTATGATTGAGGTTCTCCTCTCATTGCAACAAATGGAACCTCAGTATTATAAAGACGAGTATATTAGAGGCCTCATGCTCGTTTTGCTATTAGCCGGAACAGAAGGTTCCATCAATACAATGGAGTGGCTTCTTTCACTATTACTCAATCACCCACACTCTCTTCGACGGGCCCAAATGGAAATCGACAACGTAGTTGGAAAAACTAATCGACTCCTGGAGGAATCGGATCTGACCCACCTTCCTTACCTCCGTAGCCTGATTCACGAGACTCTTCGAATGTACCCGCCAGGCCCATTATTGATTCCACACGAATCATCGCAGGATTGCCACGTTGGAGGGTTCCATGTCCCAGCTGGCACAATGTTATTCGTCAACGTATGGGCCATTCAAAACGACCCCACCGTATGGGTGGAGCCGGAGAAATTTAACCCAGATAGATTCAAAAGCGATGGAGAGGGGTTCAAATGGATGCCATTTGGGGCTGGGCGTAGGAGGTGTCCCGGCGAGAGCTTAGGACTTCGTGTCGTTGGATTGGTCATAGGATCGCTAATTCAGTGCTTTGAATGGGAGTCAATGGGCGGAAAATGTATTGATATGAGCGAAGGTGGTGGATTGACCTTGCCCAAAGCTCTCCCCCTCCGCACCCTTTGTCGGCCACGTTCAAATGCCACACATCTGCTTTCCCAAATCTGATTCAAAATGCATGGTCTTGTTTAGTTTGTACAACAACTTTTAATTCTGTACAGAAATTTGACATATTTTAGTAGTTGAAAACATCTACATTATTTTCAATTTGTTGACGTCCTATTTTCCCTTTTTCTTTTGCCTCTTTGTTTTTTGTAATGATTGGTCGATGTCCTCTATTTTAGTAGAAGCAAAAGTACTCTTCGTGAGATAATGTTTAAATTGAGTGAATGATGAAAGAAAGAGCATCATTTATCAATTGATACTATGCTCTTCTAAGTACGTAAAATGACAAAATT

Coding sequence (CDS)

ATGGGTTTTTGCTTTTGGTTTTGCTTCTCTCAACCAAGGAAGCTATCGTTTCTACTTCGGATGGAAGATGGTTCATGGATGGTAAATTCCATGGTGATTTCATTCCTATATCTTCTAATTTTATCCATACTTCAAATCTTCACAAATCACTTCCTTCATAGAATCCGAAATCTTCCTCCAAGCCCATTTCTTTCTCTACCCATTATAGGCCAACTATACCTCCTTAAAGCCCCCCTCCACCGGACCCTATCGGAAATCTCCCGCCGTCATGGCCCAATCCTCTTCTTCCAATTCGGTTTCCGTCCAGTACTGGTCGTTTCATCTCCGTCGGCCGCCGAGGACTGCCTATCCAAAAACGACGTCATCTTCGCCAATCGACCTCGTCTTGTTAGCGGCAAATACCTCGGGTATAATTACACAAGCCTCCTATGGGCGCCATACGGAGAGCATTGGCGAAACCTCCGTCGAATCTCGTCGTTAGAAATCCTTTCCAGCCACCGCCTCCAAACGCTTTCAAGTATTCGCGTGGATGAAGTCCGTAAGCTGATCCGGAGACTGTATAATGCTGAAAACGACGTCGTAGACATGAGGACAGAGTTTTTCCAATTGATGTTCAATATCATGATGAGAATGATCGCCGGAAAACGATATTATGGGGAAGCTGTGGTAGCGGACTCGGAGGAGGGGAGGAGATTTCAAGAGATACAAGAGGAAACGTTTCGATTAAGCGGCAAGACTAATTTGGGGGACTTTTTAAGAGTTGTGAAATGGGTTGGATTATCTAAAGGGATTGAAAATAGATTGAGGGAGTTACAGATTAGGAGGGATGATTGGATGCAAAGTTTGATTGAAGAACATCGGAAGAAAATGGATAATGCTCATAGTTCTTCTTCTTCTTCTATTCAAGGTGATGCTAAAAAGACTATGATTGAGGTTCTCCTCTCATTGCAACAAATGGAACCTCAGTATTATAAAGACGAGTATATTAGAGGCCTCATGCTCGTTTTGCTATTAGCCGGAACAGAAGGTTCCATCAATACAATGGAGTGGCTTCTTTCACTATTACTCAATCACCCACACTCTCTTCGACGGGCCCAAATGGAAATCGACAACGTAGTTGGAAAAACTAATCGACTCCTGGAGGAATCGGATCTGACCCACCTTCCTTACCTCCGTAGCCTGATTCACGAGACTCTTCGAATGTACCCGCCAGGCCCATTATTGATTCCACACGAATCATCGCAGGATTGCCACGTTGGAGGGTTCCATGTCCCAGCTGGCACAATGTTATTCGTCAACGTATGGGCCATTCAAAACGACCCCACCGTATGGGTGGAGCCGGAGAAATTTAACCCAGATAGATTCAAAAGCGATGGAGAGGGGTTCAAATGGATGCCATTTGGGGCTGGGCGTAGGAGGTGTCCCGGCGAGAGCTTAGGACTTCGTGTCGTTGGATTGGTCATAGGATCGCTAATTCAGTGCTTTGAATGGGAGTCAATGGGCGGAAAATGTATTGATATGAGCGAAGGTGGTGGATTGACCTTGCCCAAAGCTCTCCCCCTCCGCACCCTTTGTCGGCCACGTTCAAATGCCACACATCTGCTTTCCCAAATCTGA

Protein sequence

MGFCFWFCFSQPRKLSFLLRMEDGSWMVNSMVISFLYLLILSILQIFTNHFLHRIRNLPPSPFLSLPIIGQLYLLKAPLHRTLSEISRRHGPILFFQFGFRPVLVVSSPSAAEDCLSKNDVIFANRPRLVSGKYLGYNYTSLLWAPYGEHWRNLRRISSLEILSSHRLQTLSSIRVDEVRKLIRRLYNAENDVVDMRTEFFQLMFNIMMRMIAGKRYYGEAVVADSEEGRRFQEIQEETFRLSGKTNLGDFLRVVKWVGLSKGIENRLRELQIRRDDWMQSLIEEHRKKMDNAHSSSSSSIQGDAKKTMIEVLLSLQQMEPQYYKDEYIRGLMLVLLLAGTEGSINTMEWLLSLLLNHPHSLRRAQMEIDNVVGKTNRLLEESDLTHLPYLRSLIHETLRMYPPGPLLIPHESSQDCHVGGFHVPAGTMLFVNVWAIQNDPTVWVEPEKFNPDRFKSDGEGFKWMPFGAGRRRCPGESLGLRVVGLVIGSLIQCFEWESMGGKCIDMSEGGGLTLPKALPLRTLCRPRSNATHLLSQI
Homology
BLAST of Cmc07g0183581 vs. NCBI nr
Match: XP_008452618.1 (PREDICTED: cytochrome P450 81E8-like [Cucumis melo])

HSP 1 Score: 1078.9 bits (2789), Expect = 0.0e+00
Identity = 534/538 (99.26%), Postives = 535/538 (99.44%), Query Frame = 0

Query: 1   MGFCFWFCFSQPRKLSFLLRMEDGSWMVNSMVISFLYLLILSILQIFTNHFLHRIRNLPP 60
           MGFCFWFCFSQPRKLSFLLRMEDGSWMVNSMVISFLYLLIL ILQIFTNHFLHRIRNLPP
Sbjct: 1   MGFCFWFCFSQPRKLSFLLRMEDGSWMVNSMVISFLYLLILFILQIFTNHFLHRIRNLPP 60

Query: 61  SPFLSLPIIGQLYLLKAPLHRTLSEISRRHGPILFFQFGFRPVLVVSSPSAAEDCLSKND 120
           SPFLSLPIIGQLYLLKAPLHRTLSEISRRHGPILFFQFGFRPVLVVSSPSA EDCLSKND
Sbjct: 61  SPFLSLPIIGQLYLLKAPLHRTLSEISRRHGPILFFQFGFRPVLVVSSPSAVEDCLSKND 120

Query: 121 VIFANRPRLVSGKYLGYNYTSLLWAPYGEHWRNLRRISSLEILSSHRLQTLSSIRVDEVR 180
           VIFANRPRLVSGKYLGYNYTSLLWAPYGEHWRNLRRISSLEILSSHRLQTLSSIRVDEVR
Sbjct: 121 VIFANRPRLVSGKYLGYNYTSLLWAPYGEHWRNLRRISSLEILSSHRLQTLSSIRVDEVR 180

Query: 181 KLIRRLYNAENDVVDMRTEFFQLMFNIMMRMIAGKRYYGEAVVADSEEGRRFQEIQEETF 240
           KLIRRLYNAENDVVDMRTEFF+LMFNIMMRMIAGKRYYGEAVVADSEEGRRFQEIQEETF
Sbjct: 181 KLIRRLYNAENDVVDMRTEFFELMFNIMMRMIAGKRYYGEAVVADSEEGRRFQEIQEETF 240

Query: 241 RLSGKTNLGDFLRVVKWVGLSKGIENRLRELQIRRDDWMQSLIEEHRKKMDNAHSSSSSS 300
           RLSGKTNLGDFLRVVKWVGLSKGIENRLRELQIRRDDWMQSLIEEHRKKMDNAHSSSSSS
Sbjct: 241 RLSGKTNLGDFLRVVKWVGLSKGIENRLRELQIRRDDWMQSLIEEHRKKMDNAHSSSSSS 300

Query: 301 IQGDAKKTMIEVLLSLQQMEPQYYKDEYIRGLMLVLLLAGTEGSINTMEWLLSLLLNHPH 360
           IQGDAKKTMIEVLLSLQQMEPQ YKDEYIRGLMLVLLLAGTEGSINTMEWLLSLLLNHPH
Sbjct: 301 IQGDAKKTMIEVLLSLQQMEPQNYKDEYIRGLMLVLLLAGTEGSINTMEWLLSLLLNHPH 360

Query: 361 SLRRAQMEIDNVVGKTNRLLEESDLTHLPYLRSLIHETLRMYPPGPLLIPHESSQDCHVG 420
           SLRRAQMEIDNVVGKTNRLLEESDLTHLPYLRSLIHETLRMYPPGPLLIPHESSQDCHVG
Sbjct: 361 SLRRAQMEIDNVVGKTNRLLEESDLTHLPYLRSLIHETLRMYPPGPLLIPHESSQDCHVG 420

Query: 421 GFHVPAGTMLFVNVWAIQNDPTVWVEPEKFNPDRFKSDGEGFKWMPFGAGRRRCPGESLG 480
           GFHVPAGTMLFVNVWAIQNDPTVWVEPEKFNPDRFKSDGEGFKWMPFGAGRRRCPGESLG
Sbjct: 421 GFHVPAGTMLFVNVWAIQNDPTVWVEPEKFNPDRFKSDGEGFKWMPFGAGRRRCPGESLG 480

Query: 481 LRVVGLVIGSLIQCFEWESMGGKCIDMSEGGGLTLPKALPLRTLCRPRSNATHLLSQI 539
           LRVVGLVIGSLIQCFEWESMGGKCIDMSEGGGLTLPKALPLRTLCRPRSNATHLLSQI
Sbjct: 481 LRVVGLVIGSLIQCFEWESMGGKCIDMSEGGGLTLPKALPLRTLCRPRSNATHLLSQI 538

BLAST of Cmc07g0183581 vs. NCBI nr
Match: XP_004141396.1 (cytochrome P450 81Q32 [Cucumis sativus] >XP_011654142.1 cytochrome P450 81Q32 [Cucumis sativus] >XP_031739532.1 cytochrome P450 81Q32 [Cucumis sativus] >KGN55248.1 hypothetical protein Csa_012192 [Cucumis sativus])

HSP 1 Score: 952.6 bits (2461), Expect = 1.5e-273
Identity = 473/508 (93.11%), Postives = 489/508 (96.26%), Query Frame = 0

Query: 31  MVISFLYLLILSILQIFTNHFLHRIRNLPPSPFLSLPIIGQLYLLKAPLHRTLSEISRRH 90
           MVI FL+LLIL +LQIFTNHFLHRIRNLPPSPFLSLPIIGQLYLLK PLHRTLSEISRRH
Sbjct: 1   MVIPFLHLLILFLLQIFTNHFLHRIRNLPPSPFLSLPIIGQLYLLKTPLHRTLSEISRRH 60

Query: 91  GPILFFQFGFRPVLVVSSPSAAEDCLSKNDVIFANRPRLVSGKYLGYNYTSLLWAPYGEH 150
           GPI+FFQFGFR VLVVSSPSAAEDCLSKNDVIFANRPRLVSGKYLGYNYTSLLWAPYGEH
Sbjct: 61  GPIVFFQFGFRSVLVVSSPSAAEDCLSKNDVIFANRPRLVSGKYLGYNYTSLLWAPYGEH 120

Query: 151 WRNLRRISSLEILSSHRLQTLSSIRVDEVRKLIRRLYNAENDVVDMRTEFFQLMFNIMMR 210
           WRNLRRISSLEILSSHRLQTLSSIRVDEVR LIRRLYNAENDVVDMRTEFF++MFNI MR
Sbjct: 121 WRNLRRISSLEILSSHRLQTLSSIRVDEVRTLIRRLYNAENDVVDMRTEFFEVMFNITMR 180

Query: 211 MIAGKRYYGEAVVADSEEGRRFQEIQEETFRLSGKTNLGDFLRVVKWVGLSKGIENRLRE 270
           MIAGKRYYGE V A SEE  RF+EIQEETFRLSGKTNLGDFLRVVKWVGLSKGIENRLRE
Sbjct: 181 MIAGKRYYGEDVAAYSEEAGRFREIQEETFRLSGKTNLGDFLRVVKWVGLSKGIENRLRE 240

Query: 271 LQIRRDDWMQSLIEEHRKKMDNAHSSSSSSIQGDAKKTMIEVLLSLQQMEPQYYKDEYIR 330
           LQI+RDDWMQSLIEEHRKKM+NA+ SSSSSIQ DAKKTMIEVLLSLQQ EPQYYKDEYIR
Sbjct: 241 LQIKRDDWMQSLIEEHRKKMNNAY-SSSSSIQTDAKKTMIEVLLSLQQKEPQYYKDEYIR 300

Query: 331 GLMLVLLLAGTEGSINTMEWLLSLLLNHPHSLRRAQMEIDNVVGKTNRLLEESDLTHLPY 390
           GLMLVLLLAGTEGSINTMEWLLSLLLNHPHSL+RAQMEID+VVG+TNRLLEESDLTHLPY
Sbjct: 301 GLMLVLLLAGTEGSINTMEWLLSLLLNHPHSLQRAQMEIDDVVGRTNRLLEESDLTHLPY 360

Query: 391 LRSLIHETLRMYPPGPLLIPHESSQDCHVGGFHVPAGTMLFVNVWAIQNDPTVWVEPEKF 450
           LRSLIHETLRMYPPGPLLIPHESS+DCHVGGFHVPAGTMLFVNVWAIQNDPTVWVEP KF
Sbjct: 361 LRSLIHETLRMYPPGPLLIPHESSEDCHVGGFHVPAGTMLFVNVWAIQNDPTVWVEPRKF 420

Query: 451 NPDRFKSDGEGFKWMPFGAGRRRCPGESLGLRVVGLVIGSLIQCFEWESMGGKCIDMSEG 510
           NPDRF  DGEGFKWMPFGAGRRRCPGE LGLRV+GLV+GSLIQCFEWESM G+CIDMSEG
Sbjct: 421 NPDRFGGDGEGFKWMPFGAGRRRCPGEGLGLRVIGLVVGSLIQCFEWESMDGECIDMSEG 480

Query: 511 GGLTLPKALPLRTLCRPRSNATHLLSQI 539
           GGLTLPKALPLRTLCRPRSNATHLLSQI
Sbjct: 481 GGLTLPKALPLRTLCRPRSNATHLLSQI 507

BLAST of Cmc07g0183581 vs. NCBI nr
Match: XP_038899302.1 (cytochrome P450 81Q32-like [Benincasa hispida])

HSP 1 Score: 900.6 bits (2326), Expect = 6.6e-258
Identity = 442/512 (86.33%), Postives = 475/512 (92.77%), Query Frame = 0

Query: 27  MVNSMVISFLYLLILSILQIFTNHFLHRIRNLPPSPFLSLPIIGQLYLLKAPLHRTLSEI 86
           M  SM I FLYLL+L ILQIFTNHFLHRIRNLPPSP+LSLPIIGQLYLLK+PLHRTLSEI
Sbjct: 1   MKMSMAIPFLYLLVLFILQIFTNHFLHRIRNLPPSPYLSLPIIGQLYLLKSPLHRTLSEI 60

Query: 87  SRRHGPILFFQFGFRPVLVVSSPSAAEDCLSKNDVIFANRPRLVSGKYLGYNYTSLLWAP 146
           + RHGPILFF+FGFR VLVVSSPSAAEDCLSKNDV+FANRPRL SGKYLGYNYTSLLWAP
Sbjct: 61  AHRHGPILFFRFGFRQVLVVSSPSAAEDCLSKNDVVFANRPRLFSGKYLGYNYTSLLWAP 120

Query: 147 YGEHWRNLRRISSLEILSSHRLQTLSSIRVDEVRKLIRRLYNAENDVVDMRTEFFQLMFN 206
           YGEHWRNLRRISSLEILS HRLQTLSSIRVDEVR LIRRLYNA+NDVVDMRTEFF+LMFN
Sbjct: 121 YGEHWRNLRRISSLEILSKHRLQTLSSIRVDEVRTLIRRLYNAKNDVVDMRTEFFELMFN 180

Query: 207 IMMRMIAGKRYYGEAVVADSEEGRRFQEIQEETFRLSGKTNLGDFLRVVKWVGLSKGIEN 266
           IMMRMIAGKRYYGE V AD+EE  RF+EIQEETFRLS KTNLGDFLRVVKWVGLSKG+EN
Sbjct: 181 IMMRMIAGKRYYGEDVAADTEEVGRFREIQEETFRLSSKTNLGDFLRVVKWVGLSKGLEN 240

Query: 267 RLRELQIRRDDWMQSLIEEHRKKMDNAHSSSSSSIQGDAKKTMIEVLLSLQQMEPQYYKD 326
           +LRELQ++RD WMQSLIEEHRK+M+N    +S+S+Q DAKKTMIEVLLSLQQMEP YYKD
Sbjct: 241 KLRELQMKRDVWMQSLIEEHRKQMNN----TSTSLQNDAKKTMIEVLLSLQQMEPHYYKD 300

Query: 327 EYIRGLMLVLLLAGTEGSINTMEWLLSLLLNHPHSLRRAQMEIDNVVGKTNRLLEESDLT 386
           E+IRGLMLVLLLAGTEGSINTMEWLLSLLLNHP  L+RAQMEID+VVG++NR+LEESDL 
Sbjct: 301 EFIRGLMLVLLLAGTEGSINTMEWLLSLLLNHPDCLQRAQMEIDDVVGRSNRILEESDLA 360

Query: 387 HLPYLRSLIHETLRMYPPGPLLIPHESSQDCHVGGFHVPAGTMLFVNVWAIQNDPTVWVE 446
           HLPYLRSLIHETLRMYPPGPLLIPHESS+DCHVGGF VPAGTMLFVNVWAIQNDPTVWVE
Sbjct: 361 HLPYLRSLIHETLRMYPPGPLLIPHESSEDCHVGGFCVPAGTMLFVNVWAIQNDPTVWVE 420

Query: 447 PEKFNPDRFKSDGEGFKWMPFGAGRRRCPGESLGLRVVGLVIGSLIQCFEWESMGGKCID 506
           P  FNP+RF S+GEGFKWMPFGAGRRRCPGE LGLRVVGL +GSLIQCF+WES+GG+C+D
Sbjct: 421 PGNFNPERFGSEGEGFKWMPFGAGRRRCPGEGLGLRVVGLAVGSLIQCFDWESVGGECVD 480

Query: 507 MSEGGGLTLPKALPLRTLCRPRSNATHLLSQI 539
           M EGGGLTLPKALPL  LCRPRSN  HLL QI
Sbjct: 481 MREGGGLTLPKALPLHALCRPRSNVAHLLPQI 508

BLAST of Cmc07g0183581 vs. NCBI nr
Match: XP_023535659.1 (isoflavone 2'-hydroxylase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 852.8 bits (2202), Expect = 1.6e-243
Identity = 422/510 (82.75%), Postives = 454/510 (89.02%), Query Frame = 0

Query: 31  MVISFLYLLILSILQIFTNHFLHRIRNLPPSPFLSLPIIGQLYLLKAPLHRTLSEISRRH 90
           M I+FL LLIL IL IFTN FLHRIRNLPPSPF SLPIIGQLYLLK PLHRTLS+ISRRH
Sbjct: 1   MAITFLCLLILLILHIFTNQFLHRIRNLPPSPFFSLPIIGQLYLLKRPLHRTLSDISRRH 60

Query: 91  GPILFFQFGFRPVLVVSSPSAAEDCLSKNDVIFANRPRLVSGKYLGYNYTSLLWAPYGEH 150
           GPILFF FGFRPVLVVSSPSAAE+CLSKND++FANRP L+ GKYLGYNYTSLLWAPYGEH
Sbjct: 61  GPILFFWFGFRPVLVVSSPSAAEECLSKNDIVFANRPHLIGGKYLGYNYTSLLWAPYGEH 120

Query: 151 WRNLRRISSLEILSSHRLQTLSSIRVDEVRKLIRRLYNAENDVVDMRTEFFQLMFNIMMR 210
           WRNLRR+SSLEI S+HRLQTLSSIRVDEVR LIRRLYN ENDVVDMRTEFF+LMFNIMMR
Sbjct: 121 WRNLRRVSSLEIFSTHRLQTLSSIRVDEVRTLIRRLYNTENDVVDMRTEFFELMFNIMMR 180

Query: 211 MIAGKRYYGEAVVADSEEGRRFQEIQEETFRLSGKTNLGDFLRVVKWVGLSKGIENRLRE 270
           MIAGKRYYGE   A+SEE  RF+EIQEETFRLS KTNLGDFLR+VKW GLSKG+ENR++E
Sbjct: 181 MIAGKRYYGED-AAESEEAERFREIQEETFRLSSKTNLGDFLRIVKWAGLSKGLENRMKE 240

Query: 271 LQIRRDDWMQSLIEEHRKKMDNAHSSSSSSIQGDAKKTMIEVLLSLQQMEPQYYKDEYIR 330
           LQ +RD WMQSLIEEHRK+            + DAKKTMIEVLLSLQQ EP+YYKDEYIR
Sbjct: 241 LQSKRDAWMQSLIEEHRKQS-----------KKDAKKTMIEVLLSLQQKEPRYYKDEYIR 300

Query: 331 GLMLVLLLAGTEGSINTMEWLLSLLLNHPHSLRRAQMEIDNVVGKTNRLLEESDLTHLPY 390
           GLMLVLLLAGTEGSINTMEWLLSLLLN+P  L+RAQ+EID+VVG++NR+LEESDL HLPY
Sbjct: 301 GLMLVLLLAGTEGSINTMEWLLSLLLNNPECLQRAQLEIDDVVGRSNRILEESDLAHLPY 360

Query: 391 LRSLIHETLRMYPPGPLLIPHESSQDCHVGGFHVPAGTMLFVNVWAIQNDPTVWVEPEKF 450
           LRSLIHETLRMYPPGPLLIPHESS DCHVGGF VPAGT LFVNVWAIQNDPTVWV+PEKF
Sbjct: 361 LRSLIHETLRMYPPGPLLIPHESSADCHVGGFRVPAGTTLFVNVWAIQNDPTVWVDPEKF 420

Query: 451 NPDRFKSDGEG--FKWMPFGAGRRRCPGESLGLRVVGLVIGSLIQCFEWESMGGKCIDMS 510
           NP RF S+GEG  FKWMPFGAGRRRCPGE LGLRVVGL +GSLIQCFEWES+GG+CIDM 
Sbjct: 421 NPGRFGSEGEGWFFKWMPFGAGRRRCPGEGLGLRVVGLAVGSLIQCFEWESVGGECIDMR 480

Query: 511 EGGGLTLPKALPLRTLCRPRSNATHLLSQI 539
           EG GLTLPKA+PL  LCRPRS   HLL QI
Sbjct: 481 EGVGLTLPKAVPLHALCRPRSKVAHLLPQI 498

BLAST of Cmc07g0183581 vs. NCBI nr
Match: KAG7024285.1 (Isoflavone 2'-hydroxylase, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 852.0 bits (2200), Expect = 2.7e-243
Identity = 423/510 (82.94%), Postives = 452/510 (88.63%), Query Frame = 0

Query: 31  MVISFLYLLILSILQIFTNHFLHRIRNLPPSPFLSLPIIGQLYLLKAPLHRTLSEISRRH 90
           M I FL LLIL IL IFTN FLHRIRNLPPSPF SLPIIGQLYLLK PLHRTLS+ISRRH
Sbjct: 1   MAIIFLCLLILLILHIFTNQFLHRIRNLPPSPFFSLPIIGQLYLLKRPLHRTLSDISRRH 60

Query: 91  GPILFFQFGFRPVLVVSSPSAAEDCLSKNDVIFANRPRLVSGKYLGYNYTSLLWAPYGEH 150
           GPILFF FGFRPVLVVSSPSAAE+CLSKND++FANRP L+ GKYLGYNYTSLLWAPYGEH
Sbjct: 61  GPILFFWFGFRPVLVVSSPSAAEECLSKNDIVFANRPHLIGGKYLGYNYTSLLWAPYGEH 120

Query: 151 WRNLRRISSLEILSSHRLQTLSSIRVDEVRKLIRRLYNAENDVVDMRTEFFQLMFNIMMR 210
           WRNLRRISSLEI S+HRLQTLSSIRVDEVR LIRRLYN +NDVVDMRTEFF+LMFNIMMR
Sbjct: 121 WRNLRRISSLEIFSTHRLQTLSSIRVDEVRTLIRRLYNTKNDVVDMRTEFFELMFNIMMR 180

Query: 211 MIAGKRYYGEAVVADSEEGRRFQEIQEETFRLSGKTNLGDFLRVVKWVGLSKGIENRLRE 270
           MIAGKRYYGE   A+SEE  RF+EIQEETFRLS KTNLGDFLR+VKW GLSKG+ENR++E
Sbjct: 181 MIAGKRYYGED-AAESEEAERFREIQEETFRLSSKTNLGDFLRIVKWAGLSKGLENRMKE 240

Query: 271 LQIRRDDWMQSLIEEHRKKMDNAHSSSSSSIQGDAKKTMIEVLLSLQQMEPQYYKDEYIR 330
           LQ +RD WMQSLIEEHRK+ +            DAKKTMIEVLLSLQQ EP YYKDEYIR
Sbjct: 241 LQSKRDAWMQSLIEEHRKQSNK-----------DAKKTMIEVLLSLQQKEPHYYKDEYIR 300

Query: 331 GLMLVLLLAGTEGSINTMEWLLSLLLNHPHSLRRAQMEIDNVVGKTNRLLEESDLTHLPY 390
           GLMLVLLLAGTEGSINTMEWLLSLLLN+P  L+RAQ EID+VVG++NR+LEESDL HLPY
Sbjct: 301 GLMLVLLLAGTEGSINTMEWLLSLLLNNPECLQRAQTEIDDVVGRSNRILEESDLAHLPY 360

Query: 391 LRSLIHETLRMYPPGPLLIPHESSQDCHVGGFHVPAGTMLFVNVWAIQNDPTVWVEPEKF 450
           LRSLIHETLRMYPPGPLLIPHESS+DCHVGGF VPAGT LFVNVWAIQNDPTVWVEPEKF
Sbjct: 361 LRSLIHETLRMYPPGPLLIPHESSEDCHVGGFRVPAGTTLFVNVWAIQNDPTVWVEPEKF 420

Query: 451 NPDRFKSDGEG--FKWMPFGAGRRRCPGESLGLRVVGLVIGSLIQCFEWESMGGKCIDMS 510
           NP RF S+GEG  FKWMPFGAGRRRCPGE LGLRVVGL +GSLIQCFEWES+GG+CIDM 
Sbjct: 421 NPGRFGSEGEGWFFKWMPFGAGRRRCPGEGLGLRVVGLAVGSLIQCFEWESVGGECIDMR 480

Query: 511 EGGGLTLPKALPLRTLCRPRSNATHLLSQI 539
           EG GLTLPKA+PL  LCRPRS   HLL QI
Sbjct: 481 EGVGLTLPKAVPLHALCRPRSKVAHLLPQI 498

BLAST of Cmc07g0183581 vs. ExPASy Swiss-Prot
Match: W8JMU7 (Cytochrome P450 81Q32 OS=Catharanthus roseus OX=4058 GN=CYP81Q32 PE=2 SV=1)

HSP 1 Score: 474.9 bits (1221), Expect = 1.2e-132
Identity = 245/514 (47.67%), Postives = 350/514 (68.09%), Query Frame = 0

Query: 29  NSMVISFLYLLILSI-LQIFTNHFLHRIRNLPPSPFLSLPIIGQLYLLKAPLHRTLSEIS 88
           ++++ +FL +++LSI L++F      R RNLPPSP L+LP+IG L+L+   LHR+L ++S
Sbjct: 4   STLLYTFLAVVLLSISLKLFP--VSRRRRNLPPSPGLALPVIGHLHLIGKLLHRSLYDLS 63

Query: 89  RRHGPILFFQFGFRPVLVVSSPSAAEDCLSKNDVIFANRPRLVSGKYLGYNYTSLLWAPY 148
           +++G +   Q G R VLVVSSP+AAE+C +KND++FANRP  + GKY+GYNYT+++ +PY
Sbjct: 64  KKYGSVFSLQLGNRLVLVVSSPAAAEECFTKNDIVFANRPLFILGKYIGYNYTTMVGSPY 123

Query: 149 GEHWRNLRRISSLEILSSHRLQTLSSIRVDEVRKLIRRLYNAEND---VVDMRTEFFQLM 208
           GEHWRNLRR++++EI S+  L    SIR DEV++L+  LY +       V+M+++  +L 
Sbjct: 124 GEHWRNLRRLAAVEIFSAGSLNRFLSIREDEVKQLLLSLYQSSGQDFGKVEMKSKLSELS 183

Query: 209 FNIMMRMIAGKRYYGEAVVADSEEGRRFQEIQEETFRLSGKTNLGDFLRVVKWVGLSKGI 268
           FN+ MRM+AGKRY+G+ V  DS+E + F+ +  E F  +G +N GDF+  ++W+   K  
Sbjct: 184 FNVTMRMVAGKRYFGQDV--DSDEAKLFRALIGEVFEHAGASNPGDFVPFLRWIDF-KNY 243

Query: 269 ENRLRELQIRRDDWMQSLIEEHRKKMDNAHSSSSSSIQGDAKKTMIEVLLSLQQMEPQYY 328
           E ++ ++    D ++Q LI E R   +N               TMI+ LLSLQ+ +P+YY
Sbjct: 244 EKKVSKISQEMDAFLQRLIHESRINKNNV--------------TMIDHLLSLQESQPEYY 303

Query: 329 KDEYIRGLMLVLLLAGTEGSINTMEWLLSLLLNHPHSLRRAQMEIDNVVGKTNRLLEESD 388
            D+ I+G+++VLLLAGT+ S  T+EW +SLLLNHP +L +A+ EI+  VG +NRL+EE D
Sbjct: 304 TDQIIKGIIMVLLLAGTDTSAVTVEWAMSLLLNHPETLEKARTEIETQVG-SNRLIEEQD 363

Query: 389 LTHLPYLRSLIHETLRMYPPGPLLIPHESSQDCHVGGFHVPAGTMLFVNVWAIQNDPTVW 448
           L  L YL ++I ET R+ P  P+L+PHESS DC V G+ VP GT+L VN WAI  DP  W
Sbjct: 364 LPKLTYLHNIISETFRLCPAAPMLVPHESSDDCKVQGYDVPKGTILLVNAWAIHRDPEFW 423

Query: 449 VEPEKFNPDRFKS-DGEGFKWMPFGAGRRRCPGESLGLRVVGLVIGSLIQCFEWESMGGK 508
            EP  F P+R    + E  K MPFG GRR CPG  L  RVVGL +G+LIQCFEW+ +G  
Sbjct: 424 DEPTLFKPERHGGVELEPSKLMPFGMGRRSCPGSGLAQRVVGLTLGALIQCFEWKRIGEA 483

Query: 509 CIDMSEGGGLTLPKALPLRTLCRPRSNATHLLSQ 538
            IDM+EG GLT+PKA PL  LC+PR+    ++S+
Sbjct: 484 KIDMAEGSGLTMPKAQPLEALCKPRNILHKVVSE 497

BLAST of Cmc07g0183581 vs. ExPASy Swiss-Prot
Match: Q6WNQ8 (Cytochrome P450 81E8 OS=Medicago truncatula OX=3880 GN=CYP81E8 PE=2 SV=1)

HSP 1 Score: 447.2 bits (1149), Expect = 2.6e-124
Identity = 231/504 (45.83%), Postives = 337/504 (66.87%), Query Frame = 0

Query: 30  SMVISFLYLLILSILQIFTNHFLHRIRNLPPSPFLSLPIIGQLYLLKAPLHRTLSEISRR 89
           S++IS  +L+I   L++F N    + +NLPP P   LPIIG L+ LK PLH T   +S++
Sbjct: 7   SLIISLFFLII--TLKVFFN-TSRKFKNLPPGP-QCLPIIGNLHQLKQPLHHTFHTLSQK 66

Query: 90  HGPILFFQFGFRPVLVVSSPSAAEDCLSKNDVIFANRPRLVSGKYLGYNYTSLLWAPYGE 149
           +G I    FG R V+VVSS + A++C +KND++ ANRP  ++GKY+GYN T++  +PYG+
Sbjct: 67  YGQIFSLWFGSRLVVVVSSLTIAQECFTKNDIVLANRPHFLTGKYIGYNNTTVAQSPYGD 126

Query: 150 HWRNLRRISSLEILSSHRLQTLSSIRVDEVRKLIRRL----YNAENDVVDMRTEFFQLMF 209
           HWRNLRRI S+EILSSHRL +   IR DE+ +LI++L    YN   + V++R  F ++ F
Sbjct: 127 HWRNLRRILSIEILSSHRLNSFLEIRRDEIMRLIQKLAQKSYNGFTE-VELRPMFSEMTF 186

Query: 210 NIMMRMIAGKRYYG-EAVVADSEEGRRFQEIQEETFRLSGKTNLGDFLRVVKWVGLSKGI 269
           N +MRM++GKRYYG +  V+D EE R F+ I +E   L G  N+GDFL  ++W     G+
Sbjct: 187 NTIMRMVSGKRYYGNDCDVSDVEEARLFRGIIKEVVSLGGANNVGDFLGFLRWFDFD-GL 246

Query: 270 ENRLRELQIRRDDWMQSLIEEHRKKMDNAHSSSSSSIQGDAKKTMIEVLLSLQQMEPQYY 329
           E RL+++  R D ++Q LI+EHR    N++             TMI+ LL+ QQ +P+YY
Sbjct: 247 EKRLKKISKRTDAFLQGLIDEHRFGKRNSN-------------TMIDHLLTQQQSQPEYY 306

Query: 330 KDEYIRGLMLVLLLAGTEGSINTMEWLLSLLLNHPHSLRRAQMEIDNVVGKTNRLLEESD 389
            D+ I+GLM+V+LLAGT+ S  T+EW +S LLNHP  +++A+ E+D  +G  +R ++E D
Sbjct: 307 TDQIIKGLMVVMLLAGTDTSSVTIEWAMSNLLNHPEIMKKAKNELDTHIGH-DRQVDEHD 366

Query: 390 LTHLPYLRSLIHETLRMYPPGPLLIPHESSQDCHVGGFHVPAGTMLFVNVWAIQNDPTVW 449
           ++ LPYL+S+++ETLR++   PLL+PH SS+D  +GG+++P  T+L VN W I  DP +W
Sbjct: 367 ISKLPYLQSIVYETLRLHAAAPLLVPHLSSEDFSLGGYNIPQNTILMVNAWVIHRDPNLW 426

Query: 450 VEPEKFNPDRFKSDGEGFKWMPFGAGRRRCPGESLGLRVVGLVIGSLIQCFEWESMGGKC 509
            +P  F P+RF+ +GE  K + FG GRR CPGE+L  R  GL +G LIQCFEW+ +G + 
Sbjct: 427 SDPTCFKPERFEKEGEVNKLLSFGLGRRACPGENLSQRTEGLTLGLLIQCFEWKRIGEEK 486

Query: 510 IDMSEGGGLTLPKALPLRTLCRPR 529
           IDM E  G+T  K   L  +C+ R
Sbjct: 487 IDMVEAKGITAGKKTSLNAMCKVR 490

BLAST of Cmc07g0183581 vs. ExPASy Swiss-Prot
Match: Q6WNQ9 (Isoflavone 3'-hydroxylase (Fragment) OS=Medicago truncatula OX=3880 GN=CYP81E9 PE=1 SV=1)

HSP 1 Score: 443.0 bits (1138), Expect = 4.9e-123
Identity = 232/505 (45.94%), Postives = 333/505 (65.94%), Query Frame = 0

Query: 32  VISFLYLLILSILQIFTNHFLHRIRNLPPSPFLSLPIIGQLYLLKAPLHRTLSEISRRHG 91
           ++S  +++ + IL   T+    R++NLPP P  ++PIIG L+ LK PLHRT + +S+ +G
Sbjct: 7   LLSLSFIITIKILLKITS---RRLKNLPPGP-PTIPIIGNLHHLKHPLHRTFTTLSQTYG 66

Query: 92  PILFFQFGFRPVLVVSSPSAAEDCLSKNDVIFANRPRLVSGKYLGYNYTSLLWAPYGEHW 151
            I    FG R V+VVSSPS A +C +KND+I ANRPR ++GKY+ YNYT+L  A YG+HW
Sbjct: 67  DIFSLWFGSRLVVVVSSPSLAHECFTKNDIILANRPRFLTGKYIFYNYTTLGSASYGDHW 126

Query: 152 RNLRRISSLEILSSHRLQTLSSIRVDEVRKLIRRLYNAENDV---------VDMRTEFFQ 211
           RNLRRI+++++LS++RL +   +R DE  +LI++L     DV         V++R    +
Sbjct: 127 RNLRRITTIDVLSNNRLNSFLGVRRDETNRLIQKLL---KDVVSEGFGFTKVELRPRLTE 186

Query: 212 LMFNIMMRMIAGKRYYG-EAVVADSEEGRRFQEIQEETFRLSGKTNLGDFLRVVKWVGLS 271
           + FN MMRMI+GKRYYG +  V+D EE ++F+EI  E   L G  N GDFL +++ V L 
Sbjct: 187 MTFNAMMRMISGKRYYGDDGDVSDVEEAKQFREIISEMMSLLGANNKGDFLPLLRVVDLD 246

Query: 272 KGIENRLRELQIRRDDWMQSLIEEHRKKMDNAHSSSSSSIQGDAKKTMIEVLLSLQQMEP 331
             +E R + +  R + +++ LIEEHR+   N HS            TMI+ LL L + +P
Sbjct: 247 -NLEKRCKRIAKRSNAFLEGLIEEHRR--GNIHSDGG---------TMIDHLLKLSESQP 306

Query: 332 QYYKDEYIRGLMLVLLLAGTEGSINTMEWLLSLLLNHPHSLRRAQMEIDNVVGKTNRLLE 391
           +YY D  I+GL+  +LLAGT+ S  T+EW++S LLNHP  L++A+ E+D  +GK N+L++
Sbjct: 307 EYYSDHLIKGLIQGMLLAGTDTSAVTIEWVMSELLNHPEVLKKAKEELDTQIGK-NKLVD 366

Query: 392 ESDLTHLPYLRSLIHETLRMYPPGPLLIPHESSQDCHVGGFHVPAGTMLFVNVWAIQNDP 451
           E DL+ LPYL+++I ETLR++PP PLL+PH SS+DC +G F+VP  T++  NVW I  DP
Sbjct: 367 EQDLSKLPYLQNIISETLRLHPPAPLLLPHYSSEDCTIGEFNVPKDTIILTNVWGIHRDP 426

Query: 452 TVWVEPEKFNPDRFKSDGEGFKWMPFGAGRRRCPGESLGLRVVGLVIGSLIQCFEWESMG 511
             W +   F P+RF+ + E  K M FG GRR CPG SL  R VG  +G LIQCFEWE   
Sbjct: 427 KHWNDALSFKPERFEKEEEVNKVMAFGLGRRACPGLSLAQRTVGFTVGLLIQCFEWERES 486

Query: 512 GKCIDMSEGGGLTLPKALPLRTLCR 527
            + +DM EG G+T+P  +PLR +C+
Sbjct: 487 EEKLDMMEGKGITMPMKIPLRAMCK 491

BLAST of Cmc07g0183581 vs. ExPASy Swiss-Prot
Match: P93147 (Isoflavone 2'-hydroxylase OS=Glycyrrhiza echinata OX=46348 GN=CYP81E1 PE=1 SV=2)

HSP 1 Score: 437.6 bits (1124), Expect = 2.1e-121
Identity = 221/499 (44.29%), Postives = 328/499 (65.73%), Query Frame = 0

Query: 34  SFLYLLILSILQIFTNHFLHRIRNLPPSPFLSLPIIGQLYLLKAPLHRTLSEISRRHGPI 93
           S  YL +  I  I       + +NLPP P  SLPIIG L+ LK PLHRT   +S ++G +
Sbjct: 10  SVFYLALFFIFNIVIR--ARKFKNLPPGP-PSLPIIGNLHHLKRPLHRTFKGLSEKYGHV 69

Query: 94  LFFQFGFRPVLVVSSPSAAEDCLSKNDVIFANRPRLVSGKYLGYNYTSLLWAPYGEHWRN 153
               FG R V+VVSS S  + C +KNDV+ ANRPR +SGKY+ YNYT+L    YGEHWRN
Sbjct: 70  FSLWFGSRLVVVVSSASEFQQCFTKNDVVLANRPRFLSGKYIFYNYTTLGSTSYGEHWRN 129

Query: 154 LRRISSLEILSSHRLQTLSSIRVDEVRKLIRRLYNAEND---VVDMRTEFFQLMFNIMMR 213
           LRRI++L++LS+HR+ + S IR DE ++LI RL +  +     +++ +  + + FN +MR
Sbjct: 130 LRRITALDVLSNHRINSFSGIRRDETQRLITRLADDSSTNFAEMELSSRLYDMTFNNIMR 189

Query: 214 MIAGKRYYGE-AVVADSEEGRRFQEIQEETFRLSGKTNLGDFLRVVKWVGLSKGIENRLR 273
           MI+GKRYYGE    +D +E  +F+++  E  +LSG  N  DF+ +++++   + +E RL+
Sbjct: 190 MISGKRYYGEDCDTSDLQEASQFRDMVSELLQLSGANNKTDFMPLLRFLDF-ENLEKRLK 249

Query: 274 ELQIRRDDWMQSLIEEHRKKMDNAHSSSSSSIQGDAKKTMIEVLLSLQQMEPQYYKDEYI 333
           ++  + D +++ LIEEHR K + A+             TMI+ LL+LQ  +P+YY D+ I
Sbjct: 250 DISGKTDAFLRGLIEEHRTKKERAN-------------TMIDHLLNLQDSQPEYYTDQII 309

Query: 334 RGLMLVLLLAGTEGSINTMEWLLSLLLNHPHSLRRAQMEIDNVVGKTNRLLEESDLTHLP 393
           +GL L +LLAGT+ S  T+EW +S LLNHP  L++ + E+D  VG+ +RL++ESDL  L 
Sbjct: 310 KGLALAMLLAGTDSSAVTLEWSMSNLLNHPEVLKKVKDELDTHVGQ-DRLVDESDLPKLT 369

Query: 394 YLRSLIHETLRMYPPGPLLIPHESSQDCHVGGFHVPAGTMLFVNVWAIQNDPTVWVEPEK 453
           YL+++I+ETLR+Y P PLL+PH +S +C++GG+ VP  T++ +N WAI  DP +W E   
Sbjct: 370 YLKNVINETLRLYTPAPLLLPHSTSDECNIGGYKVPQDTIVLINAWAIHRDPELWTEATT 429

Query: 454 FNPDRFKSDGEGFKWMPFGAGRRRCPGESLGLRVVGLVIGSLIQCFEWESMGGKCIDMSE 513
           F P+RF+  GE  K + FG GRR CPGE L +R + + +  LIQCF+W+ + G  ID++E
Sbjct: 430 FKPERFEKKGELEKLIAFGMGRRACPGEGLAIRAISMTLALLIQCFDWKLINGDKIDLAE 489

Query: 514 GGGLTLPKALPLRTLCRPR 529
             G TL K +PL+ +C+ R
Sbjct: 490 RDGFTLTKLVPLKAMCKSR 490

BLAST of Cmc07g0183581 vs. ExPASy Swiss-Prot
Match: Q9FG65 (Cytochrome P450 81D1 OS=Arabidopsis thaliana OX=3702 GN=CYP81D1 PE=2 SV=1)

HSP 1 Score: 424.9 bits (1091), Expect = 1.4e-117
Identity = 225/503 (44.73%), Postives = 322/503 (64.02%), Query Frame = 0

Query: 36  LYLLILSILQIFTNHFLH-RIRNLPPSPFLSLPIIGQLYLLKAPLHRTLSEISRR----- 95
           LY +   I  I +  FL  + +NLPPSP   LPIIG L LLK P+HRTL   S       
Sbjct: 10  LYSIFSLIFLIISFKFLKPKKQNLPPSPPGWLPIIGHLRLLKPPIHRTLRSFSETLDHND 69

Query: 96  HGPILFFQFGFRPVLVVSSPS-AAEDCLSKNDVIFANRPRLVSGKYLGYNYTSLLWAPYG 155
            G ++  + G R V VVSS   AAE+C  KNDV+ ANRP+++ GK++GYN T+++ APYG
Sbjct: 70  GGGVMSLRLGSRLVYVVSSHKVAAEECFGKNDVVLANRPQVIIGKHVGYNNTNMIAAPYG 129

Query: 156 EHWRNLRRISSLEILSSHRLQTLSSIRVDEVRKLI---RRLYNAENDVVDMRTEFFQLMF 215
           +HWRNLRR+ ++EI S+HRL     +R DEVR+LI    RL   +  VV+++     L F
Sbjct: 130 DHWRNLRRLCTIEIFSTHRLNCFLYVRTDEVRRLISRLSRLAGTKKTVVELKPMLMDLTF 189

Query: 216 NIMMRMIAGKRYYGEAVVADSEEGRRFQEIQEETFRLSGKTNLGDFLRVVKWVGLSKGIE 275
           N +MRM+ GKRYYGE    D EE +R +++  +    +   N  D++ +++   L    E
Sbjct: 190 NNIMRMMTGKRYYGEE-TTDEEEAKRVRKLVADVGANTSSGNAVDYVPILR---LFSSYE 249

Query: 276 NRLRELQIRRDDWMQSLIEEHRKKMDNAHSSSSSSIQGDAKKTMIEVLLSLQQMEPQYYK 335
           NR+++L    D ++Q LI++ R              Q +   TMI+ LL LQ+ + +YY 
Sbjct: 250 NRVKKLGEETDKFLQGLIDDKRG-------------QQETGTTMIDHLLVLQKSDIEYYT 309

Query: 336 DEYIRGLMLVLLLAGTEGSINTMEWLLSLLLNHPHSLRRAQMEIDNVVGKTNRLLEESDL 395
           D+ I+G++L++++AGT  S  T+EW LS LLNHP  + +A+ EIDN VG  +RL+EE+DL
Sbjct: 310 DQIIKGIILIMVIAGTNTSAVTLEWALSNLLNHPDVISKARDEIDNRVG-LDRLIEEADL 369

Query: 396 THLPYLRSLIHETLRMYPPGPLLIPHESSQDCHVGGFHVPAGTMLFVNVWAIQNDPTVWV 455
           + LPYL++++ ETLR++P  PLL+PH +S+DC +G + +P GT L VN WAI  DP  W 
Sbjct: 370 SELPYLKNIVLETLRLHPATPLLVPHMASEDCKIGSYDMPRGTTLLVNAWAIHRDPNTWD 429

Query: 456 EPEKFNPDRFKSDGEGFKWMPFGAGRRRCPGESLGLRVVGLVIGSLIQCFEWESMGGKCI 515
           +P+ F P+RF+ + E  K + FG GRR CPG  L  R+VGL +GSLIQCFEWE +G   +
Sbjct: 430 DPDSFKPERFEKEEEAQKLLAFGLGRRACPGSGLAQRIVGLALGSLIQCFEWERVGNVEV 489

Query: 516 DMSEGGGLTLPKALPLRTLCRPR 529
           DM EG G T+PKA+PL+ +C+ R
Sbjct: 490 DMKEGVGNTVPKAIPLKAICKAR 494

BLAST of Cmc07g0183581 vs. ExPASy TrEMBL
Match: A0A1S3BVF3 (cytochrome P450 81E8-like OS=Cucumis melo OX=3656 GN=LOC103493585 PE=3 SV=1)

HSP 1 Score: 1078.9 bits (2789), Expect = 0.0e+00
Identity = 534/538 (99.26%), Postives = 535/538 (99.44%), Query Frame = 0

Query: 1   MGFCFWFCFSQPRKLSFLLRMEDGSWMVNSMVISFLYLLILSILQIFTNHFLHRIRNLPP 60
           MGFCFWFCFSQPRKLSFLLRMEDGSWMVNSMVISFLYLLIL ILQIFTNHFLHRIRNLPP
Sbjct: 1   MGFCFWFCFSQPRKLSFLLRMEDGSWMVNSMVISFLYLLILFILQIFTNHFLHRIRNLPP 60

Query: 61  SPFLSLPIIGQLYLLKAPLHRTLSEISRRHGPILFFQFGFRPVLVVSSPSAAEDCLSKND 120
           SPFLSLPIIGQLYLLKAPLHRTLSEISRRHGPILFFQFGFRPVLVVSSPSA EDCLSKND
Sbjct: 61  SPFLSLPIIGQLYLLKAPLHRTLSEISRRHGPILFFQFGFRPVLVVSSPSAVEDCLSKND 120

Query: 121 VIFANRPRLVSGKYLGYNYTSLLWAPYGEHWRNLRRISSLEILSSHRLQTLSSIRVDEVR 180
           VIFANRPRLVSGKYLGYNYTSLLWAPYGEHWRNLRRISSLEILSSHRLQTLSSIRVDEVR
Sbjct: 121 VIFANRPRLVSGKYLGYNYTSLLWAPYGEHWRNLRRISSLEILSSHRLQTLSSIRVDEVR 180

Query: 181 KLIRRLYNAENDVVDMRTEFFQLMFNIMMRMIAGKRYYGEAVVADSEEGRRFQEIQEETF 240
           KLIRRLYNAENDVVDMRTEFF+LMFNIMMRMIAGKRYYGEAVVADSEEGRRFQEIQEETF
Sbjct: 181 KLIRRLYNAENDVVDMRTEFFELMFNIMMRMIAGKRYYGEAVVADSEEGRRFQEIQEETF 240

Query: 241 RLSGKTNLGDFLRVVKWVGLSKGIENRLRELQIRRDDWMQSLIEEHRKKMDNAHSSSSSS 300
           RLSGKTNLGDFLRVVKWVGLSKGIENRLRELQIRRDDWMQSLIEEHRKKMDNAHSSSSSS
Sbjct: 241 RLSGKTNLGDFLRVVKWVGLSKGIENRLRELQIRRDDWMQSLIEEHRKKMDNAHSSSSSS 300

Query: 301 IQGDAKKTMIEVLLSLQQMEPQYYKDEYIRGLMLVLLLAGTEGSINTMEWLLSLLLNHPH 360
           IQGDAKKTMIEVLLSLQQMEPQ YKDEYIRGLMLVLLLAGTEGSINTMEWLLSLLLNHPH
Sbjct: 301 IQGDAKKTMIEVLLSLQQMEPQNYKDEYIRGLMLVLLLAGTEGSINTMEWLLSLLLNHPH 360

Query: 361 SLRRAQMEIDNVVGKTNRLLEESDLTHLPYLRSLIHETLRMYPPGPLLIPHESSQDCHVG 420
           SLRRAQMEIDNVVGKTNRLLEESDLTHLPYLRSLIHETLRMYPPGPLLIPHESSQDCHVG
Sbjct: 361 SLRRAQMEIDNVVGKTNRLLEESDLTHLPYLRSLIHETLRMYPPGPLLIPHESSQDCHVG 420

Query: 421 GFHVPAGTMLFVNVWAIQNDPTVWVEPEKFNPDRFKSDGEGFKWMPFGAGRRRCPGESLG 480
           GFHVPAGTMLFVNVWAIQNDPTVWVEPEKFNPDRFKSDGEGFKWMPFGAGRRRCPGESLG
Sbjct: 421 GFHVPAGTMLFVNVWAIQNDPTVWVEPEKFNPDRFKSDGEGFKWMPFGAGRRRCPGESLG 480

Query: 481 LRVVGLVIGSLIQCFEWESMGGKCIDMSEGGGLTLPKALPLRTLCRPRSNATHLLSQI 539
           LRVVGLVIGSLIQCFEWESMGGKCIDMSEGGGLTLPKALPLRTLCRPRSNATHLLSQI
Sbjct: 481 LRVVGLVIGSLIQCFEWESMGGKCIDMSEGGGLTLPKALPLRTLCRPRSNATHLLSQI 538

BLAST of Cmc07g0183581 vs. ExPASy TrEMBL
Match: A0A0A0L0F1 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G642290 PE=3 SV=1)

HSP 1 Score: 952.6 bits (2461), Expect = 7.1e-274
Identity = 473/508 (93.11%), Postives = 489/508 (96.26%), Query Frame = 0

Query: 31  MVISFLYLLILSILQIFTNHFLHRIRNLPPSPFLSLPIIGQLYLLKAPLHRTLSEISRRH 90
           MVI FL+LLIL +LQIFTNHFLHRIRNLPPSPFLSLPIIGQLYLLK PLHRTLSEISRRH
Sbjct: 1   MVIPFLHLLILFLLQIFTNHFLHRIRNLPPSPFLSLPIIGQLYLLKTPLHRTLSEISRRH 60

Query: 91  GPILFFQFGFRPVLVVSSPSAAEDCLSKNDVIFANRPRLVSGKYLGYNYTSLLWAPYGEH 150
           GPI+FFQFGFR VLVVSSPSAAEDCLSKNDVIFANRPRLVSGKYLGYNYTSLLWAPYGEH
Sbjct: 61  GPIVFFQFGFRSVLVVSSPSAAEDCLSKNDVIFANRPRLVSGKYLGYNYTSLLWAPYGEH 120

Query: 151 WRNLRRISSLEILSSHRLQTLSSIRVDEVRKLIRRLYNAENDVVDMRTEFFQLMFNIMMR 210
           WRNLRRISSLEILSSHRLQTLSSIRVDEVR LIRRLYNAENDVVDMRTEFF++MFNI MR
Sbjct: 121 WRNLRRISSLEILSSHRLQTLSSIRVDEVRTLIRRLYNAENDVVDMRTEFFEVMFNITMR 180

Query: 211 MIAGKRYYGEAVVADSEEGRRFQEIQEETFRLSGKTNLGDFLRVVKWVGLSKGIENRLRE 270
           MIAGKRYYGE V A SEE  RF+EIQEETFRLSGKTNLGDFLRVVKWVGLSKGIENRLRE
Sbjct: 181 MIAGKRYYGEDVAAYSEEAGRFREIQEETFRLSGKTNLGDFLRVVKWVGLSKGIENRLRE 240

Query: 271 LQIRRDDWMQSLIEEHRKKMDNAHSSSSSSIQGDAKKTMIEVLLSLQQMEPQYYKDEYIR 330
           LQI+RDDWMQSLIEEHRKKM+NA+ SSSSSIQ DAKKTMIEVLLSLQQ EPQYYKDEYIR
Sbjct: 241 LQIKRDDWMQSLIEEHRKKMNNAY-SSSSSIQTDAKKTMIEVLLSLQQKEPQYYKDEYIR 300

Query: 331 GLMLVLLLAGTEGSINTMEWLLSLLLNHPHSLRRAQMEIDNVVGKTNRLLEESDLTHLPY 390
           GLMLVLLLAGTEGSINTMEWLLSLLLNHPHSL+RAQMEID+VVG+TNRLLEESDLTHLPY
Sbjct: 301 GLMLVLLLAGTEGSINTMEWLLSLLLNHPHSLQRAQMEIDDVVGRTNRLLEESDLTHLPY 360

Query: 391 LRSLIHETLRMYPPGPLLIPHESSQDCHVGGFHVPAGTMLFVNVWAIQNDPTVWVEPEKF 450
           LRSLIHETLRMYPPGPLLIPHESS+DCHVGGFHVPAGTMLFVNVWAIQNDPTVWVEP KF
Sbjct: 361 LRSLIHETLRMYPPGPLLIPHESSEDCHVGGFHVPAGTMLFVNVWAIQNDPTVWVEPRKF 420

Query: 451 NPDRFKSDGEGFKWMPFGAGRRRCPGESLGLRVVGLVIGSLIQCFEWESMGGKCIDMSEG 510
           NPDRF  DGEGFKWMPFGAGRRRCPGE LGLRV+GLV+GSLIQCFEWESM G+CIDMSEG
Sbjct: 421 NPDRFGGDGEGFKWMPFGAGRRRCPGEGLGLRVIGLVVGSLIQCFEWESMDGECIDMSEG 480

Query: 511 GGLTLPKALPLRTLCRPRSNATHLLSQI 539
           GGLTLPKALPLRTLCRPRSNATHLLSQI
Sbjct: 481 GGLTLPKALPLRTLCRPRSNATHLLSQI 507

BLAST of Cmc07g0183581 vs. ExPASy TrEMBL
Match: A0A6J1FED9 (isoflavone 2'-hydroxylase-like OS=Cucurbita moschata OX=3662 GN=LOC111443311 PE=3 SV=1)

HSP 1 Score: 850.1 bits (2195), Expect = 5.0e-243
Identity = 422/510 (82.75%), Postives = 452/510 (88.63%), Query Frame = 0

Query: 31  MVISFLYLLILSILQIFTNHFLHRIRNLPPSPFLSLPIIGQLYLLKAPLHRTLSEISRRH 90
           M I FL LLIL IL IFTN FLHRIRNLPPSPF SLPIIGQLYLLK PLHRTLS+ISRRH
Sbjct: 1   MAIIFLCLLILLILHIFTNQFLHRIRNLPPSPFFSLPIIGQLYLLKRPLHRTLSDISRRH 60

Query: 91  GPILFFQFGFRPVLVVSSPSAAEDCLSKNDVIFANRPRLVSGKYLGYNYTSLLWAPYGEH 150
           GPILFF FGFRPVLVVSSPSAAE+CLSKND++FANRP L+ GKYLGYNYTSLLWAPYGEH
Sbjct: 61  GPILFFWFGFRPVLVVSSPSAAEECLSKNDIVFANRPHLIGGKYLGYNYTSLLWAPYGEH 120

Query: 151 WRNLRRISSLEILSSHRLQTLSSIRVDEVRKLIRRLYNAENDVVDMRTEFFQLMFNIMMR 210
           WRNLRRISSLEI S+HRLQTLSSIRVDEVR LIRRLYN +NDVVDMRTEFF+LMFNIMMR
Sbjct: 121 WRNLRRISSLEIFSTHRLQTLSSIRVDEVRTLIRRLYNTKNDVVDMRTEFFELMFNIMMR 180

Query: 211 MIAGKRYYGEAVVADSEEGRRFQEIQEETFRLSGKTNLGDFLRVVKWVGLSKGIENRLRE 270
           MIAGKRYYGE   A+SEE  RF+EIQEETFRLS KTNLGDFLR+VKW GLSKG+ENR++E
Sbjct: 181 MIAGKRYYGED-AAESEEAERFREIQEETFRLSSKTNLGDFLRIVKWAGLSKGLENRMKE 240

Query: 271 LQIRRDDWMQSLIEEHRKKMDNAHSSSSSSIQGDAKKTMIEVLLSLQQMEPQYYKDEYIR 330
           LQ +RD WMQSLIEEHRK+            + DAKKTMIEVLLSLQQ EP YYKDEYIR
Sbjct: 241 LQSKRDAWMQSLIEEHRKQS-----------KKDAKKTMIEVLLSLQQKEPHYYKDEYIR 300

Query: 331 GLMLVLLLAGTEGSINTMEWLLSLLLNHPHSLRRAQMEIDNVVGKTNRLLEESDLTHLPY 390
           GLMLVLLLAGTEGSINTMEWLLSLLLN+P  L+RAQ EID+VVG++NR+LEESDL HLPY
Sbjct: 301 GLMLVLLLAGTEGSINTMEWLLSLLLNNPECLQRAQTEIDDVVGRSNRILEESDLAHLPY 360

Query: 391 LRSLIHETLRMYPPGPLLIPHESSQDCHVGGFHVPAGTMLFVNVWAIQNDPTVWVEPEKF 450
           LRSLIHETLRMYPPGPLLIPHESS+DCHVGGF VPAGT LFVNVWAIQNDPTVWV+PEKF
Sbjct: 361 LRSLIHETLRMYPPGPLLIPHESSEDCHVGGFRVPAGTTLFVNVWAIQNDPTVWVDPEKF 420

Query: 451 NPDRFKSDGEG--FKWMPFGAGRRRCPGESLGLRVVGLVIGSLIQCFEWESMGGKCIDMS 510
           NP RF S+GEG  FKWMPFGAGRRRCPGE LGLRVVGL +GSLIQCFEWES+GG+CIDM 
Sbjct: 421 NPGRFGSEGEGWFFKWMPFGAGRRRCPGEGLGLRVVGLAVGSLIQCFEWESVGGECIDMR 480

Query: 511 EGGGLTLPKALPLRTLCRPRSNATHLLSQI 539
           EG GLTLPKA+PL  LCRPRS   HLL QI
Sbjct: 481 EGVGLTLPKAVPLHALCRPRSKVAHLLPQI 498

BLAST of Cmc07g0183581 vs. ExPASy TrEMBL
Match: A0A6J1INS3 (isoflavone 2'-hydroxylase-like OS=Cucurbita maxima OX=3661 GN=LOC111477207 PE=3 SV=1)

HSP 1 Score: 847.4 bits (2188), Expect = 3.2e-242
Identity = 421/510 (82.55%), Postives = 452/510 (88.63%), Query Frame = 0

Query: 31  MVISFLYLLILSILQIFTNHFLHRIRNLPPSPFLSLPIIGQLYLLKAPLHRTLSEISRRH 90
           M I+FL LLIL IL IFTN FLHRIRNLPPSPF SLPIIGQLYLLK PLHRTL +ISRRH
Sbjct: 1   MAITFLCLLILFILHIFTNQFLHRIRNLPPSPFFSLPIIGQLYLLKRPLHRTLLDISRRH 60

Query: 91  GPILFFQFGFRPVLVVSSPSAAEDCLSKNDVIFANRPRLVSGKYLGYNYTSLLWAPYGEH 150
           GPILFF FGFRPVLVVSSPSAAE+CLSKND++FANRP L+ GKYLGYNYTSLLWAPYGEH
Sbjct: 61  GPILFFWFGFRPVLVVSSPSAAEECLSKNDIVFANRPHLIGGKYLGYNYTSLLWAPYGEH 120

Query: 151 WRNLRRISSLEILSSHRLQTLSSIRVDEVRKLIRRLYNAENDVVDMRTEFFQLMFNIMMR 210
           WRNLRRISSLEI S+HRLQTLSSIRVDEVR LIRRLYN +NDVVDM+TEFF+LMFNIMMR
Sbjct: 121 WRNLRRISSLEIFSTHRLQTLSSIRVDEVRTLIRRLYNTKNDVVDMKTEFFELMFNIMMR 180

Query: 211 MIAGKRYYGEAVVADSEEGRRFQEIQEETFRLSGKTNLGDFLRVVKWVGLSKGIENRLRE 270
           MIAGKRYYGE  VA+SEE  RF+EIQEETFRLS KTNLGDFLR+VKW GLSKG+ENR++E
Sbjct: 181 MIAGKRYYGED-VAESEEAERFREIQEETFRLSSKTNLGDFLRMVKWAGLSKGLENRMKE 240

Query: 271 LQIRRDDWMQSLIEEHRKKMDNAHSSSSSSIQGDAKKTMIEVLLSLQQMEPQYYKDEYIR 330
           LQ +RD WMQSLIEEHRK+            + DAKKTMIEVLLSLQQ EP YYKDEYIR
Sbjct: 241 LQSKRDAWMQSLIEEHRKQS-----------KKDAKKTMIEVLLSLQQKEPHYYKDEYIR 300

Query: 331 GLMLVLLLAGTEGSINTMEWLLSLLLNHPHSLRRAQMEIDNVVGKTNRLLEESDLTHLPY 390
           GLMLVLLLAGTEGSINTMEWLLSLLLN+P  L+RAQ EID+VVG++NR+LEESDL HLPY
Sbjct: 301 GLMLVLLLAGTEGSINTMEWLLSLLLNNPECLQRAQTEIDDVVGRSNRILEESDLAHLPY 360

Query: 391 LRSLIHETLRMYPPGPLLIPHESSQDCHVGGFHVPAGTMLFVNVWAIQNDPTVWVEPEKF 450
           LRSLIHETLRMYPPGPLLIPHESS DCHVGGF VPAGT LFVNVWAIQNDPTVWV+PEKF
Sbjct: 361 LRSLIHETLRMYPPGPLLIPHESSADCHVGGFRVPAGTTLFVNVWAIQNDPTVWVDPEKF 420

Query: 451 NPDRFKSDGEG--FKWMPFGAGRRRCPGESLGLRVVGLVIGSLIQCFEWESMGGKCIDMS 510
           NP RF S+GEG  FKWMPFGAGRRRCPGE LGLRVVGL +GSLIQCFEWES+GG+CIDM 
Sbjct: 421 NPGRFGSEGEGWFFKWMPFGAGRRRCPGEGLGLRVVGLAVGSLIQCFEWESVGGECIDMR 480

Query: 511 EGGGLTLPKALPLRTLCRPRSNATHLLSQI 539
           EG GLTLPKA+PL  LCRPRS   HLL QI
Sbjct: 481 EGVGLTLPKAVPLHALCRPRSKVAHLLPQI 498

BLAST of Cmc07g0183581 vs. ExPASy TrEMBL
Match: A0A6P4BCT8 (cytochrome P450 81E8-like OS=Ziziphus jujuba OX=326968 GN=LOC107433678 PE=3 SV=1)

HSP 1 Score: 651.4 bits (1679), Expect = 3.4e-183
Identity = 326/514 (63.42%), Postives = 405/514 (78.79%), Query Frame = 0

Query: 35  FLYLLILSILQIFTNHFLHRIRNLPPSPFLSLPIIGQLYLLKAPLHRTLSEISRRHGPIL 94
           F Y+L+ S + +FT H  H+I+NLPPSPF  LP+IG LYLL+ PLHRTLS+++ R+GPIL
Sbjct: 6   FFYILLFSAVYVFTKHLFHKIQNLPPSPF-PLPVIGHLYLLERPLHRTLSKLAGRYGPIL 65

Query: 95  FFQFGFRPVLVVSSPSAAEDCLSKNDVIFANRPRLVSGKYLGYNYTSLLWAPYGEHWRNL 154
           F + G RPVLVVSS SAAE+CL+KND+IFANRPRL+ GKYLGYN+TS+ WAPYG+HWRNL
Sbjct: 66  FLKLGSRPVLVVSSSSAAEECLAKNDIIFANRPRLMVGKYLGYNFTSVAWAPYGDHWRNL 125

Query: 155 RRISSLEILSSHRLQTLSSIRVDEVRKLIRRLYNAENDVVDMRTEFFQLMFNIMMRMIAG 214
           RRISSLE+LSSHRLQ LS IR++EV+ L+RRL+  +  VVDMR  FF+L  NIMMRMIAG
Sbjct: 126 RRISSLELLSSHRLQMLSGIRLEEVKLLLRRLFEKQGVVVDMRQAFFELTLNIMMRMIAG 185

Query: 215 KRYYGEAVVADSEEGRRFQEIQEETFRLSGKTNLGDFLRVVKWVGLSKGIENRLRELQIR 274
           KRYYG+ VV D +E  RF+EIQEET RLSGK+NLGDFL  V+W+G SK +E  L ELQ +
Sbjct: 186 KRYYGDEVVEDLKEAMRFREIQEETVRLSGKSNLGDFLPFVRWIGGSKRLEKSLMELQRK 245

Query: 275 RDDWMQSLIEEHRKKM-----DNAHSSSSSSIQGDAKKTMIEVLLSLQQMEPQYYKDEYI 334
           RD ++Q LIEEHR+       D    S S S   + +KTM+EVLLSLQQMEP+YYKDE I
Sbjct: 246 RDGFLQDLIEEHRRTASRIANDCCSPSPSPSKSKERRKTMLEVLLSLQQMEPEYYKDEII 305

Query: 335 RGLMLVLLLAGTEGSINTMEWLLSLLLNHPHSLRRAQMEIDNVVGKTNRLLEESDLTHLP 394
           RGLMLVLLL GT+ +INT++W LSLLLNHP  L++AQ EIDN VG   RL++ESD+  LP
Sbjct: 306 RGLMLVLLLGGTDTTINTLQWSLSLLLNHPEILKKAQDEIDNRVGH-GRLVDESDVAQLP 365

Query: 395 YLRSLIHETLRMYPPGPLLIPHESSQDCHVGGFHVPAGTMLFVNVWAIQNDPTVWVEPEK 454
           YL+S+I+ETLRMYPP PLL+PHESS++  VGGF +P GT L VN+WAIQNDPT+WV+P  
Sbjct: 366 YLKSIINETLRMYPPIPLLLPHESSEESTVGGFRIPRGTTLLVNIWAIQNDPTIWVDPTS 425

Query: 455 FNPDRF-KSDG---EGFKWMPFGAGRRRCPGESLGLRVVGLVIGSLIQCFEWESMG-GKC 514
           F P+R+ +++G   +  + MPFG+GRR CPGE LG+RVVGLV+G +IQCF WE +   K 
Sbjct: 426 FKPERYIEAEGMRNDKLRLMPFGSGRRGCPGEGLGMRVVGLVLGLIIQCFNWERVSQEKM 485

Query: 515 IDMSEGGGLTLPKALPLRTLCRPRSNATHLLSQI 539
           +DMSEG GLTLPK+ PL+  C PRS  + L+SQI
Sbjct: 486 VDMSEGNGLTLPKSQPLQAKCWPRSTMSKLVSQI 517

BLAST of Cmc07g0183581 vs. TAIR 10
Match: AT4G37340.1 (cytochrome P450, family 81, subfamily D, polypeptide 3 )

HSP 1 Score: 448.0 bits (1151), Expect = 1.1e-125
Identity = 233/514 (45.33%), Postives = 334/514 (64.98%), Query Frame = 0

Query: 30  SMVISFLYLLILSILQIFTNHFLHRIRNLPPSPFLSLPIIGQLYLLKAPLHRTLSEISRR 89
           +++ +FL+   +S+   F    + R  NLPPSP  +LP+IG L LLK PLHR    +S  
Sbjct: 5   TLIFTFLF---ISLSLTFIIGRIKRRPNLPPSPSWALPVIGHLRLLKPPLHRVFLSVSES 64

Query: 90  HG--PILFFQFGFRPVLVVSSPSAAEDCLSKNDVIFANRPRLVSGKYLGYNYTSLLWAPY 149
            G  PI+  + G R V VVSS S AE+C +KNDV+ ANR   ++ K++ Y  T+++ A Y
Sbjct: 65  LGDAPIISLRLGNRLVFVVSSHSLAEECFTKNDVVLANRFNSLASKHISYGCTTVVTASY 124

Query: 150 GEHWRNLRRISSLEILSSHRLQTLSSIRVDEVRKLI---RRLYNAENDVVDMRTEFFQLM 209
           G+HWRNLRRI ++EI S+HRL + SSIR DE+ +LI    R  + E   V+M++ F  L 
Sbjct: 125 GDHWRNLRRIGAVEIFSAHRLNSFSSIRRDEIHRLIACLSRNSSLEFTKVEMKSMFSNLT 184

Query: 210 FNIMMRMIAGKRYYGEAVVADSEEGRRFQEIQEETFRLSGKTNLGDFLRVVKWVGLSKGI 269
           FN ++RM+AGK YYG+    D  E +R +E+  E     G  N  D+L ++ W+    G 
Sbjct: 185 FNNIIRMLAGKCYYGDG-AEDDPEAKRVRELIAEGMGCFGAGNTADYLPILTWI---TGS 244

Query: 270 ENRLRELQIRRDDWMQSLIEEHRKKMDNAHSSSSSSIQGDAKKTMIEVLLSLQQMEPQYY 329
           E R++++  R D+++Q L++E R+  +              + TM++ LL LQ+ +P+YY
Sbjct: 245 EKRIKKIASRLDEFLQGLVDERREGKEK------------RQNTMVDHLLCLQETQPEYY 304

Query: 330 KDEYIRGLMLVLLLAGTEGSINTMEWLLSLLLNHPHSLRRAQMEIDNVVGKTNRLLEESD 389
            D  I+G+ML L+LAGT+ S  T+EW LS LLNHP  L +A+ EIDN VG  NRL+EESD
Sbjct: 305 TDNIIKGIMLSLILAGTDTSAVTLEWTLSALLNHPQILSKARDEIDNKVG-LNRLVEESD 364

Query: 390 LTHLPYLRSLIHETLRMYPPGPLLIPHESSQDCHVGGFHVPAGTMLFVNVWAIQNDPTVW 449
           L+HLPYL++++ E+LR+YP  PLL+PH +S+DC VGG+H+P GTML  N WAI  DP +W
Sbjct: 365 LSHLPYLQNIVSESLRLYPASPLLVPHVASEDCKVGGYHMPRGTMLLTNAWAIHRDPKIW 424

Query: 450 VEPEKFNPDRFKSDGEGFKWMPFGAGRRRCPGESLGLRVVGLVIGSLIQCFEWESMGGKC 509
            +P  F P+RF+ +GE  K + FG GRR CPG  L  R+  L IGSLIQCFEWE +G + 
Sbjct: 425 DDPTSFKPERFEKEGEAQKLLGFGLGRRACPGSGLAQRLASLTIGSLIQCFEWERIGEEE 484

Query: 510 IDMSE-GGGLTLPKALPLRTLCRPRSNATHLLSQ 538
           +DM+E GGG+ +PKA+PL  +C+ R     +L++
Sbjct: 485 VDMTEGGGGVIMPKAIPLVAMCKARPVVGKILNE 498

BLAST of Cmc07g0183581 vs. TAIR 10
Match: AT4G37320.1 (cytochrome P450, family 81, subfamily D, polypeptide 5 )

HSP 1 Score: 447.6 bits (1150), Expect = 1.4e-125
Identity = 233/504 (46.23%), Postives = 333/504 (66.07%), Query Frame = 0

Query: 31  MVISFLYLLILSILQIFTNHFLHRIRNLPPSPFLSLPIIGQLYLLKAPLHRTLSEISRRH 90
           +++SFL+L I SI  + T    +R  NLPPSP   LP+IG L+LLK P+HRT   IS+  
Sbjct: 7   LLLSFLFLTI-SIKLLLTKS--NRKPNLPPSPAYPLPVIGHLHLLKQPVHRTFHSISKSL 66

Query: 91  G--PILFFQFGFRPVLVVSSPSAAEDCLSKNDVIFANRPRLVSGKYLGYNYTSLLWAPYG 150
           G  PI   + G R V V+SS S AE+C +KNDV+ ANRP ++  K++GYN+T+++ A YG
Sbjct: 67  GNAPIFHLRLGNRLVYVISSHSIAEECFTKNDVVLANRPDIIMAKHVGYNFTNMIAASYG 126

Query: 151 EHWRNLRRISSLEILSSHRLQTLSSIRVDEVRKLIRRLYNAEND---VVDMRTEFFQLMF 210
           +HWRNLRRI+++EI SSHR+ T SSIR DE+R+LI  L          V++++    L F
Sbjct: 127 DHWRNLRRIAAVEIFSSHRISTFSSIRKDEIRRLITHLSRDSLHGFVEVELKSLLTNLAF 186

Query: 211 NIMMRMIAGKRYYGEAVVADSEEGRRFQEIQEETFRLSGKTNLGDFLRVVKWVGLSKGIE 270
           N ++ M+AGKRYYG     D++E +  +E+  E    +G  NL D+L  + WV      E
Sbjct: 187 NNIIMMVAGKRYYGTG-TEDNDEAKLVRELIAEIMAGAGSGNLADYLPSINWV---TNFE 246

Query: 271 NRLRELQIRRDDWMQSLIEEHRKKMDNAHSSSSSSIQGDAKKTMIEVLLSLQQMEPQYYK 330
           N+ + L  R D  +Q L++E R + +               +T+I+ LLS Q+ EP+YY 
Sbjct: 247 NQTKILGNRLDRVLQKLVDEKRAEKEKG-------------QTLIDHLLSFQETEPEYYT 306

Query: 331 DEYIRGLMLVLLLAGTEGSINTMEWLLSLLLNHPHSLRRAQMEIDNVVGKTNRLLEESDL 390
           D  I+G++L L+LAGT+ S  T+EW +S LLNHP  L +A+ EID+ +G ++RL+EESD+
Sbjct: 307 DVIIKGIILALVLAGTDTSSVTLEWAMSNLLNHPEILEKARAEIDDKIG-SDRLVEESDI 366

Query: 391 THLPYLRSLIHETLRMYPPGPLLIPHESSQDCHVGGFHVPAGTMLFVNVWAIQNDPTVWV 450
            +L YL++++ ETLR+YP  PLL+PH SS +C V G+ +P  T+L  NVWA+  DP +W 
Sbjct: 367 VNLHYLQNIVSETLRLYPAVPLLLPHFSSDECKVAGYDMPRRTLLLTNVWAMHRDPGLWE 426

Query: 451 EPEKFNPDRFKSDGEGFKWMPFGAGRRRCPGESLGLRVVGLVIGSLIQCFEWESMGGKCI 510
           EPE+F P+RF+ +GE  K MPFG GRR CPG  LG R+V L +G LIQ FEWE +G + +
Sbjct: 427 EPERFKPERFEKEGEARKLMPFGMGRRACPGAELGKRLVSLALGCLIQSFEWERVGAELV 486

Query: 511 DMSEGGGLTLPKALPLRTLCRPRS 530
           DM+EG G+T+PKA PLR +C+ R+
Sbjct: 487 DMTEGEGITMPKATPLRAMCKARA 489

BLAST of Cmc07g0183581 vs. TAIR 10
Match: AT4G37330.1 (cytochrome P450, family 81, subfamily D, polypeptide 4 )

HSP 1 Score: 443.7 bits (1140), Expect = 2.1e-124
Identity = 232/510 (45.49%), Postives = 330/510 (64.71%), Query Frame = 0

Query: 31  MVISFLYLLILSILQIFTNHFLHRIRNLPPSPFLSLPIIGQLYLLKAPLHRTLSEISRRH 90
           +++SFL L  L+I  +  +    R  NLPPSP +SLP+IG L+LLK PLHRT   +S+  
Sbjct: 7   LILSFLSLFTLAIFLLTRS---KRKLNLPPSPAISLPVIGHLHLLKPPLHRTFLSLSKSI 66

Query: 91  G--PILFFQFGFRPVLVVSSPSAAEDCLSKNDVIFANRPRLVSGKYLGYNYTSLLWAPYG 150
           G  P+   + G R V V+SS S AE+C +KNDV+ ANRP+    K+LGYN T LL A YG
Sbjct: 67  GNAPVFHLRLGNRLVYVISSRSIAEECFTKNDVVLANRPKFTISKHLGYNATYLLSASYG 126

Query: 151 EHWRNLRRISSLEILSSHRLQTLSSIRVDEVRKLIRRLYNAEND---VVDMRTEFFQLMF 210
           +HWRNLRRI+++EI S+HRL +   IR DE+R+LI  L          V+M+T    L  
Sbjct: 127 DHWRNLRRIAAVEIFSTHRLNSFLYIRKDEIRRLISHLSRDSLHGFVEVEMKTLLTNLAS 186

Query: 211 NIMMRMIAGKRYYGEAVVADSEEGRRFQEIQEETFRLSGKTNLGDFLRVVKWVGLSKGIE 270
           N  +RM+AGKRY+GE    D+++ +  + +  E    +G  N  D+L +++WV      E
Sbjct: 187 NTTIRMLAGKRYFGE----DNDDAKLVKNLVSEAVTSAGAGNPIDYLSILRWV---SSYE 246

Query: 271 NRLRELQIRRDDWMQSLIEEHRKKMDNAHSSSSSSIQGDAKKTMIEVLLSLQQMEPQYYK 330
            R++ L  R D ++Q L++E R + +               +TMI+ LL+LQ ++P YY 
Sbjct: 247 KRIKNLGNRFDTFLQKLVDEKRAEKEKG-------------ETMIDHLLALQDIQPDYYT 306

Query: 331 DEYIRGLMLVLLLAGTEGSINTMEWLLSLLLNHPHSLRRAQMEIDNVVGKTNRLLEESDL 390
           D  I+G++L L++AGT+ S  T+EW +S LLNHP  L++A+MEID  VG  +RL++ESD+
Sbjct: 307 DVIIKGIILTLIIAGTDTSSVTLEWAMSNLLNHPEILKKARMEIDEKVG-LDRLVDESDI 366

Query: 391 THLPYLRSLIHETLRMYPPGPLLIPHESSQDCHVGGFHVPAGTMLFVNVWAIQNDPTVWV 450
            +L YL+S++ ETLRMYP  PLL+PH SS+DC VGG+ +P+GTM+  N WA+  DP VW 
Sbjct: 367 VNLSYLQSIVLETLRMYPAVPLLLPHLSSEDCKVGGYDIPSGTMVLTNAWAMHRDPEVWE 426

Query: 451 EPEKFNPDRFKSDGEGFKWMPFGAGRRRCPGESLGLRVVGLVIGSLIQCFEWESMGGKCI 510
           +PE F P+RF+ +GE  K + FG GRR CPG  L  R++   +GSL+QCFEWE +G   +
Sbjct: 427 DPEIFKPERFEKEGEAEKLISFGMGRRACPGAGLAHRLINQALGSLVQCFEWERVGEDFV 486

Query: 511 DMSEGGGLTLPKALPLRTLCRPRSNATHLL 536
           DM+E  G TLPKA+PLR +C+ RS    L+
Sbjct: 487 DMTEDKGATLPKAIPLRAMCKARSIVDKLI 492

BLAST of Cmc07g0183581 vs. TAIR 10
Match: AT4G37370.1 (cytochrome P450, family 81, subfamily D, polypeptide 8 )

HSP 1 Score: 443.7 bits (1140), Expect = 2.1e-124
Identity = 229/513 (44.64%), Postives = 338/513 (65.89%), Query Frame = 0

Query: 30  SMVISFLYLLILSILQIFTNHFLHRIRNLPPSPFLSLPIIGQLYLLKAPLHRTLSEISR- 89
           +++ S L+ ++LS++ +     L R  NLPPSP  SLP+IG L LLK P+HRT   +S+ 
Sbjct: 5   TLIFSILF-VVLSLIYLIGK--LKRKPNLPPSPAWSLPVIGHLRLLKPPIHRTFLSLSQS 64

Query: 90  -RHGPILFFQFGFRPVLVVSSPSAAEDCLSKNDVIFANRPRLVSGKYLGYNYTSLLWAPY 149
             + PI   + G R V V SS S AE+C +KNDV+ ANRP  +  K++ Y+YT+++ A Y
Sbjct: 65  LNNAPIFSLRLGNRLVFVNSSHSIAEECFTKNDVVLANRPNFILAKHVAYDYTTMIAASY 124

Query: 150 GEHWRNLRRISSLEILSSHRLQTLSSIRVDEVRKLIRRL---YNAENDVVDMRTEFFQLM 209
           G+HWRNLRRI S+EI S+HRL +  SIR DE+R+L+ RL   ++ E   VDM++    L 
Sbjct: 125 GDHWRNLRRIGSVEIFSNHRLNSFLSIRKDEIRRLVFRLSRNFSQEFVKVDMKSMLSDLT 184

Query: 210 FNIMMRMIAGKRYYGEAVVADSEEGRRFQEIQEETFRLSGKTNLGDFLRVVKWVGLSKGI 269
           FN ++RM+AGKRYYG+  V D  E +R +++  +    +G  N  D+L V++   L    
Sbjct: 185 FNNILRMVAGKRYYGDG-VEDDPEAKRVRQLIADVVACAGAGNAVDYLPVLR---LVSDY 244

Query: 270 ENRLRELQIRRDDWMQSLIEEHRKKMDNAHSSSSSSIQGDAKKTMIEVLLSLQQMEPQYY 329
           E R+++L  R D+++Q L++E R+  +  +             TMI+ LL+LQ+ +P Y+
Sbjct: 245 ETRVKKLAGRLDEFLQGLVDEKREAKEKGN-------------TMIDHLLTLQESQPDYF 304

Query: 330 KDEYIRGLMLVLLLAGTEGSINTMEWLLSLLLNHPHSLRRAQMEIDNVVGKTNRLLEESD 389
            D  I+G ML L+LAGT+ S  T+EW LS +LNHP  L +A+ EID  +G  +RL++ESD
Sbjct: 305 TDRIIKGNMLALILAGTDTSAVTLEWALSNVLNHPDVLNKARDEIDRKIG-LDRLMDESD 364

Query: 390 LTHLPYLRSLIHETLRMYPPGPLLIPHESSQDCHVGGFHVPAGTMLFVNVWAIQNDPTVW 449
           +++LPYL++++ ETLR+YP  P+L+PH +S+DC V G+ +P GT+L  NVWAI  DP +W
Sbjct: 365 ISNLPYLQNIVSETLRLYPAAPMLLPHVASEDCKVAGYDMPRGTILLTNVWAIHRDPQLW 424

Query: 450 VEPEKFNPDRFKSDGEGFKWMPFGAGRRRCPGESLGLRVVGLVIGSLIQCFEWESMGGKC 509
            +P  F P+RF+ +GE  K MPFG GRR CPG  L  R++ L +GSLIQC EWE +G + 
Sbjct: 425 DDPMSFKPERFEKEGEAQKLMPFGLGRRACPGSGLAHRLINLTLGSLIQCLEWEKIGEE- 484

Query: 510 IDMSEGGGLTLPKALPLRTLCRPRSNATHLLSQ 538
           +DMSEG G+T+PKA PL  +CR R +   + ++
Sbjct: 485 VDMSEGKGVTMPKAKPLEAMCRARPSVVKIFNE 495

BLAST of Cmc07g0183581 vs. TAIR 10
Match: AT4G37360.1 (cytochrome P450, family 81, subfamily D, polypeptide 2 )

HSP 1 Score: 439.5 bits (1129), Expect = 3.9e-123
Identity = 234/505 (46.34%), Postives = 327/505 (64.75%), Query Frame = 0

Query: 31  MVISFLYLLILSILQIFTNHFLHRIRNLPPSPFLSLPIIGQLYLLKAPLHRTLSEISRRH 90
           M+I     ++LS+  IF    + R  NLPPSP  +LP+IG L LLK PLHR    +S+  
Sbjct: 5   MLIFTFCFIVLSL--IFLIGRIKRKLNLPPSPAWALPVIGHLRLLKPPLHRVFLSVSQSL 64

Query: 91  G--PILFFQFGFRPVLVVSSPSAAEDCLSKNDVIFANRPRLVSGKYLGYNYTSLLWAPYG 150
           G  PI+  + G R + VVSS S AE+C +KNDVI ANR   +S K++ Y  ++++ A Y 
Sbjct: 65  GDAPIISLRLGNRLLFVVSSHSIAEECFTKNDVILANRQTTISTKHISYGNSTVVSASYS 124

Query: 151 EHWRNLRRISSLEILSSHRLQTLSSIRVDEVRKLIRRLYNAEN---DVVDMRTEFFQLMF 210
           EHWRNLRRI +LEI S+HRL + SSIR DE+R+LI RL    +     V+M++ F  L F
Sbjct: 125 EHWRNLRRIGALEIFSAHRLNSFSSIRRDEIRRLIGRLLRNSSYGFTKVEMKSMFSDLTF 184

Query: 211 NIMMRMIAGKRYYGEAVVADSEEGRRFQEIQEETFRLSGKTNLGDFLRVVKWVGLSKGIE 270
           N ++RM+AGK YYG+    D  E +R + +  E    SG  N  D++ ++ W+  S   E
Sbjct: 185 NNIIRMLAGKCYYGDG-KEDDPEAKRVRTLIAEAMSSSGPGNAADYIPILTWITYS---E 244

Query: 271 NRLRELQIRRDDWMQSLIEEHRKKMDNAHSSSSSSIQGDAKK--TMIEVLLSLQQMEPQY 330
            R+++L  R D+++Q L++E R              +G  KK  TM++ LL LQ+ +P+Y
Sbjct: 245 TRIKKLAGRLDEFLQGLVDEKR--------------EGKEKKENTMVDHLLCLQETQPEY 304

Query: 331 YKDEYIRGLMLVLLLAGTEGSINTMEWLLSLLLNHPHSLRRAQMEIDNVVGKTNRLLEES 390
           Y D  I+G ML L+  GT+ +  T+EW LS LLN+P  L +A+ EID ++G  +RLLEES
Sbjct: 305 YMDRIIKGTMLSLIAGGTDTTAVTLEWALSSLLNNPEVLNKARDEIDRMIG-VDRLLEES 364

Query: 391 DLTHLPYLRSLIHETLRMYPPGPLLIPHESSQDCHVGGFHVPAGTMLFVNVWAIQNDPTV 450
           D+ +LPYL++++ ETLR+YP  P+L+PH +S+DC VGG+ +P GTML  N WAI  DP +
Sbjct: 365 DIPNLPYLQNIVSETLRLYPAAPMLLPHVASKDCKVGGYDMPRGTMLLTNAWAIHRDPLL 424

Query: 451 WVEPEKFNPDRFKSDGEGFKWMPFGAGRRRCPGESLGLRVVGLVIGSLIQCFEWESMGGK 510
           W +P  F P+RF+ +GE  K MPFG GRR CPG  L  R+V L +GSLIQCFEWE +G +
Sbjct: 425 WDDPTSFKPERFEKEGEAKKLMPFGLGRRACPGSGLAQRLVTLSLGSLIQCFEWERIGEE 484

Query: 511 CIDMSEGGGLTLPKALPLRTLCRPR 529
            +DM+EG GLT+PKA PL  +CR R
Sbjct: 485 EVDMTEGPGLTMPKARPLEAMCRAR 488

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_008452618.10.0e+0099.26PREDICTED: cytochrome P450 81E8-like [Cucumis melo][more]
XP_004141396.11.5e-27393.11cytochrome P450 81Q32 [Cucumis sativus] >XP_011654142.1 cytochrome P450 81Q32 [C... [more]
XP_038899302.16.6e-25886.33cytochrome P450 81Q32-like [Benincasa hispida][more]
XP_023535659.11.6e-24382.75isoflavone 2'-hydroxylase-like [Cucurbita pepo subsp. pepo][more]
KAG7024285.12.7e-24382.94Isoflavone 2'-hydroxylase, partial [Cucurbita argyrosperma subsp. argyrosperma][more]
Match NameE-valueIdentityDescription
W8JMU71.2e-13247.67Cytochrome P450 81Q32 OS=Catharanthus roseus OX=4058 GN=CYP81Q32 PE=2 SV=1[more]
Q6WNQ82.6e-12445.83Cytochrome P450 81E8 OS=Medicago truncatula OX=3880 GN=CYP81E8 PE=2 SV=1[more]
Q6WNQ94.9e-12345.94Isoflavone 3'-hydroxylase (Fragment) OS=Medicago truncatula OX=3880 GN=CYP81E9 P... [more]
P931472.1e-12144.29Isoflavone 2'-hydroxylase OS=Glycyrrhiza echinata OX=46348 GN=CYP81E1 PE=1 SV=2[more]
Q9FG651.4e-11744.73Cytochrome P450 81D1 OS=Arabidopsis thaliana OX=3702 GN=CYP81D1 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A1S3BVF30.0e+0099.26cytochrome P450 81E8-like OS=Cucumis melo OX=3656 GN=LOC103493585 PE=3 SV=1[more]
A0A0A0L0F17.1e-27493.11Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G642290 PE=3 SV=1[more]
A0A6J1FED95.0e-24382.75isoflavone 2'-hydroxylase-like OS=Cucurbita moschata OX=3662 GN=LOC111443311 PE=... [more]
A0A6J1INS33.2e-24282.55isoflavone 2'-hydroxylase-like OS=Cucurbita maxima OX=3661 GN=LOC111477207 PE=3 ... [more]
A0A6P4BCT83.4e-18363.42cytochrome P450 81E8-like OS=Ziziphus jujuba OX=326968 GN=LOC107433678 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT4G37340.11.1e-12545.33cytochrome P450, family 81, subfamily D, polypeptide 3 [more]
AT4G37320.11.4e-12546.23cytochrome P450, family 81, subfamily D, polypeptide 5 [more]
AT4G37330.12.1e-12445.49cytochrome P450, family 81, subfamily D, polypeptide 4 [more]
AT4G37370.12.1e-12444.64cytochrome P450, family 81, subfamily D, polypeptide 8 [more]
AT4G37360.13.9e-12346.34cytochrome P450, family 81, subfamily D, polypeptide 2 [more]
InterPro
Analysis Name: InterPro Annotations of Melon (Charmono) v1.1
Date Performed: 2022-10-13
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001128Cytochrome P450PRINTSPR00385P450coord: 339..356
score: 31.08
coord: 474..485
score: 30.94
coord: 393..404
score: 40.54
coord: 465..474
score: 60.07
IPR001128Cytochrome P450PFAMPF00067p450coord: 60..517
e-value: 7.9E-89
score: 298.5
IPR002401Cytochrome P450, E-class, group IPRINTSPR00463EP450Icoord: 111..132
score: 25.45
coord: 392..410
score: 34.78
coord: 203..221
score: 21.47
coord: 348..374
score: 36.36
coord: 328..345
score: 26.01
coord: 464..474
score: 57.48
coord: 87..106
score: 31.53
coord: 433..457
score: 36.64
coord: 474..497
score: 34.21
IPR036396Cytochrome P450 superfamilyGENE3D1.10.630.10Cytochrome P450coord: 53..536
e-value: 5.5E-119
score: 399.8
IPR036396Cytochrome P450 superfamilySUPERFAMILY48264Cytochrome P450coord: 59..528
NoneNo IPR availablePIRSRPIRSR000047-1PIRSR000047-1coord: 31..498
e-value: 6.7E-16
score: 56.1
NoneNo IPR availablePANTHERPTHR47947:SF24CYTOCHROME P450 81E8coord: 43..537
NoneNo IPR availablePANTHERPTHR47947CYTOCHROME P450 82C3-RELATEDcoord: 43..537
IPR017972Cytochrome P450, conserved sitePROSITEPS00086CYTOCHROME_P450coord: 467..476

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cmc07g0183581.1Cmc07g0183581.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0098542 defense response to other organism
cellular_component GO:0016020 membrane
molecular_function GO:0020037 heme binding
molecular_function GO:0005506 iron ion binding
molecular_function GO:0004497 monooxygenase activity
molecular_function GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen