Homology
BLAST of Cmc07g0183581 vs. NCBI nr
Match:
XP_008452618.1 (PREDICTED: cytochrome P450 81E8-like [Cucumis melo])
HSP 1 Score: 1078.9 bits (2789), Expect = 0.0e+00
Identity = 534/538 (99.26%), Postives = 535/538 (99.44%), Query Frame = 0
Query: 1 MGFCFWFCFSQPRKLSFLLRMEDGSWMVNSMVISFLYLLILSILQIFTNHFLHRIRNLPP 60
MGFCFWFCFSQPRKLSFLLRMEDGSWMVNSMVISFLYLLIL ILQIFTNHFLHRIRNLPP
Sbjct: 1 MGFCFWFCFSQPRKLSFLLRMEDGSWMVNSMVISFLYLLILFILQIFTNHFLHRIRNLPP 60
Query: 61 SPFLSLPIIGQLYLLKAPLHRTLSEISRRHGPILFFQFGFRPVLVVSSPSAAEDCLSKND 120
SPFLSLPIIGQLYLLKAPLHRTLSEISRRHGPILFFQFGFRPVLVVSSPSA EDCLSKND
Sbjct: 61 SPFLSLPIIGQLYLLKAPLHRTLSEISRRHGPILFFQFGFRPVLVVSSPSAVEDCLSKND 120
Query: 121 VIFANRPRLVSGKYLGYNYTSLLWAPYGEHWRNLRRISSLEILSSHRLQTLSSIRVDEVR 180
VIFANRPRLVSGKYLGYNYTSLLWAPYGEHWRNLRRISSLEILSSHRLQTLSSIRVDEVR
Sbjct: 121 VIFANRPRLVSGKYLGYNYTSLLWAPYGEHWRNLRRISSLEILSSHRLQTLSSIRVDEVR 180
Query: 181 KLIRRLYNAENDVVDMRTEFFQLMFNIMMRMIAGKRYYGEAVVADSEEGRRFQEIQEETF 240
KLIRRLYNAENDVVDMRTEFF+LMFNIMMRMIAGKRYYGEAVVADSEEGRRFQEIQEETF
Sbjct: 181 KLIRRLYNAENDVVDMRTEFFELMFNIMMRMIAGKRYYGEAVVADSEEGRRFQEIQEETF 240
Query: 241 RLSGKTNLGDFLRVVKWVGLSKGIENRLRELQIRRDDWMQSLIEEHRKKMDNAHSSSSSS 300
RLSGKTNLGDFLRVVKWVGLSKGIENRLRELQIRRDDWMQSLIEEHRKKMDNAHSSSSSS
Sbjct: 241 RLSGKTNLGDFLRVVKWVGLSKGIENRLRELQIRRDDWMQSLIEEHRKKMDNAHSSSSSS 300
Query: 301 IQGDAKKTMIEVLLSLQQMEPQYYKDEYIRGLMLVLLLAGTEGSINTMEWLLSLLLNHPH 360
IQGDAKKTMIEVLLSLQQMEPQ YKDEYIRGLMLVLLLAGTEGSINTMEWLLSLLLNHPH
Sbjct: 301 IQGDAKKTMIEVLLSLQQMEPQNYKDEYIRGLMLVLLLAGTEGSINTMEWLLSLLLNHPH 360
Query: 361 SLRRAQMEIDNVVGKTNRLLEESDLTHLPYLRSLIHETLRMYPPGPLLIPHESSQDCHVG 420
SLRRAQMEIDNVVGKTNRLLEESDLTHLPYLRSLIHETLRMYPPGPLLIPHESSQDCHVG
Sbjct: 361 SLRRAQMEIDNVVGKTNRLLEESDLTHLPYLRSLIHETLRMYPPGPLLIPHESSQDCHVG 420
Query: 421 GFHVPAGTMLFVNVWAIQNDPTVWVEPEKFNPDRFKSDGEGFKWMPFGAGRRRCPGESLG 480
GFHVPAGTMLFVNVWAIQNDPTVWVEPEKFNPDRFKSDGEGFKWMPFGAGRRRCPGESLG
Sbjct: 421 GFHVPAGTMLFVNVWAIQNDPTVWVEPEKFNPDRFKSDGEGFKWMPFGAGRRRCPGESLG 480
Query: 481 LRVVGLVIGSLIQCFEWESMGGKCIDMSEGGGLTLPKALPLRTLCRPRSNATHLLSQI 539
LRVVGLVIGSLIQCFEWESMGGKCIDMSEGGGLTLPKALPLRTLCRPRSNATHLLSQI
Sbjct: 481 LRVVGLVIGSLIQCFEWESMGGKCIDMSEGGGLTLPKALPLRTLCRPRSNATHLLSQI 538
BLAST of Cmc07g0183581 vs. NCBI nr
Match:
XP_004141396.1 (cytochrome P450 81Q32 [Cucumis sativus] >XP_011654142.1 cytochrome P450 81Q32 [Cucumis sativus] >XP_031739532.1 cytochrome P450 81Q32 [Cucumis sativus] >KGN55248.1 hypothetical protein Csa_012192 [Cucumis sativus])
HSP 1 Score: 952.6 bits (2461), Expect = 1.5e-273
Identity = 473/508 (93.11%), Postives = 489/508 (96.26%), Query Frame = 0
Query: 31 MVISFLYLLILSILQIFTNHFLHRIRNLPPSPFLSLPIIGQLYLLKAPLHRTLSEISRRH 90
MVI FL+LLIL +LQIFTNHFLHRIRNLPPSPFLSLPIIGQLYLLK PLHRTLSEISRRH
Sbjct: 1 MVIPFLHLLILFLLQIFTNHFLHRIRNLPPSPFLSLPIIGQLYLLKTPLHRTLSEISRRH 60
Query: 91 GPILFFQFGFRPVLVVSSPSAAEDCLSKNDVIFANRPRLVSGKYLGYNYTSLLWAPYGEH 150
GPI+FFQFGFR VLVVSSPSAAEDCLSKNDVIFANRPRLVSGKYLGYNYTSLLWAPYGEH
Sbjct: 61 GPIVFFQFGFRSVLVVSSPSAAEDCLSKNDVIFANRPRLVSGKYLGYNYTSLLWAPYGEH 120
Query: 151 WRNLRRISSLEILSSHRLQTLSSIRVDEVRKLIRRLYNAENDVVDMRTEFFQLMFNIMMR 210
WRNLRRISSLEILSSHRLQTLSSIRVDEVR LIRRLYNAENDVVDMRTEFF++MFNI MR
Sbjct: 121 WRNLRRISSLEILSSHRLQTLSSIRVDEVRTLIRRLYNAENDVVDMRTEFFEVMFNITMR 180
Query: 211 MIAGKRYYGEAVVADSEEGRRFQEIQEETFRLSGKTNLGDFLRVVKWVGLSKGIENRLRE 270
MIAGKRYYGE V A SEE RF+EIQEETFRLSGKTNLGDFLRVVKWVGLSKGIENRLRE
Sbjct: 181 MIAGKRYYGEDVAAYSEEAGRFREIQEETFRLSGKTNLGDFLRVVKWVGLSKGIENRLRE 240
Query: 271 LQIRRDDWMQSLIEEHRKKMDNAHSSSSSSIQGDAKKTMIEVLLSLQQMEPQYYKDEYIR 330
LQI+RDDWMQSLIEEHRKKM+NA+ SSSSSIQ DAKKTMIEVLLSLQQ EPQYYKDEYIR
Sbjct: 241 LQIKRDDWMQSLIEEHRKKMNNAY-SSSSSIQTDAKKTMIEVLLSLQQKEPQYYKDEYIR 300
Query: 331 GLMLVLLLAGTEGSINTMEWLLSLLLNHPHSLRRAQMEIDNVVGKTNRLLEESDLTHLPY 390
GLMLVLLLAGTEGSINTMEWLLSLLLNHPHSL+RAQMEID+VVG+TNRLLEESDLTHLPY
Sbjct: 301 GLMLVLLLAGTEGSINTMEWLLSLLLNHPHSLQRAQMEIDDVVGRTNRLLEESDLTHLPY 360
Query: 391 LRSLIHETLRMYPPGPLLIPHESSQDCHVGGFHVPAGTMLFVNVWAIQNDPTVWVEPEKF 450
LRSLIHETLRMYPPGPLLIPHESS+DCHVGGFHVPAGTMLFVNVWAIQNDPTVWVEP KF
Sbjct: 361 LRSLIHETLRMYPPGPLLIPHESSEDCHVGGFHVPAGTMLFVNVWAIQNDPTVWVEPRKF 420
Query: 451 NPDRFKSDGEGFKWMPFGAGRRRCPGESLGLRVVGLVIGSLIQCFEWESMGGKCIDMSEG 510
NPDRF DGEGFKWMPFGAGRRRCPGE LGLRV+GLV+GSLIQCFEWESM G+CIDMSEG
Sbjct: 421 NPDRFGGDGEGFKWMPFGAGRRRCPGEGLGLRVIGLVVGSLIQCFEWESMDGECIDMSEG 480
Query: 511 GGLTLPKALPLRTLCRPRSNATHLLSQI 539
GGLTLPKALPLRTLCRPRSNATHLLSQI
Sbjct: 481 GGLTLPKALPLRTLCRPRSNATHLLSQI 507
BLAST of Cmc07g0183581 vs. NCBI nr
Match:
XP_038899302.1 (cytochrome P450 81Q32-like [Benincasa hispida])
HSP 1 Score: 900.6 bits (2326), Expect = 6.6e-258
Identity = 442/512 (86.33%), Postives = 475/512 (92.77%), Query Frame = 0
Query: 27 MVNSMVISFLYLLILSILQIFTNHFLHRIRNLPPSPFLSLPIIGQLYLLKAPLHRTLSEI 86
M SM I FLYLL+L ILQIFTNHFLHRIRNLPPSP+LSLPIIGQLYLLK+PLHRTLSEI
Sbjct: 1 MKMSMAIPFLYLLVLFILQIFTNHFLHRIRNLPPSPYLSLPIIGQLYLLKSPLHRTLSEI 60
Query: 87 SRRHGPILFFQFGFRPVLVVSSPSAAEDCLSKNDVIFANRPRLVSGKYLGYNYTSLLWAP 146
+ RHGPILFF+FGFR VLVVSSPSAAEDCLSKNDV+FANRPRL SGKYLGYNYTSLLWAP
Sbjct: 61 AHRHGPILFFRFGFRQVLVVSSPSAAEDCLSKNDVVFANRPRLFSGKYLGYNYTSLLWAP 120
Query: 147 YGEHWRNLRRISSLEILSSHRLQTLSSIRVDEVRKLIRRLYNAENDVVDMRTEFFQLMFN 206
YGEHWRNLRRISSLEILS HRLQTLSSIRVDEVR LIRRLYNA+NDVVDMRTEFF+LMFN
Sbjct: 121 YGEHWRNLRRISSLEILSKHRLQTLSSIRVDEVRTLIRRLYNAKNDVVDMRTEFFELMFN 180
Query: 207 IMMRMIAGKRYYGEAVVADSEEGRRFQEIQEETFRLSGKTNLGDFLRVVKWVGLSKGIEN 266
IMMRMIAGKRYYGE V AD+EE RF+EIQEETFRLS KTNLGDFLRVVKWVGLSKG+EN
Sbjct: 181 IMMRMIAGKRYYGEDVAADTEEVGRFREIQEETFRLSSKTNLGDFLRVVKWVGLSKGLEN 240
Query: 267 RLRELQIRRDDWMQSLIEEHRKKMDNAHSSSSSSIQGDAKKTMIEVLLSLQQMEPQYYKD 326
+LRELQ++RD WMQSLIEEHRK+M+N +S+S+Q DAKKTMIEVLLSLQQMEP YYKD
Sbjct: 241 KLRELQMKRDVWMQSLIEEHRKQMNN----TSTSLQNDAKKTMIEVLLSLQQMEPHYYKD 300
Query: 327 EYIRGLMLVLLLAGTEGSINTMEWLLSLLLNHPHSLRRAQMEIDNVVGKTNRLLEESDLT 386
E+IRGLMLVLLLAGTEGSINTMEWLLSLLLNHP L+RAQMEID+VVG++NR+LEESDL
Sbjct: 301 EFIRGLMLVLLLAGTEGSINTMEWLLSLLLNHPDCLQRAQMEIDDVVGRSNRILEESDLA 360
Query: 387 HLPYLRSLIHETLRMYPPGPLLIPHESSQDCHVGGFHVPAGTMLFVNVWAIQNDPTVWVE 446
HLPYLRSLIHETLRMYPPGPLLIPHESS+DCHVGGF VPAGTMLFVNVWAIQNDPTVWVE
Sbjct: 361 HLPYLRSLIHETLRMYPPGPLLIPHESSEDCHVGGFCVPAGTMLFVNVWAIQNDPTVWVE 420
Query: 447 PEKFNPDRFKSDGEGFKWMPFGAGRRRCPGESLGLRVVGLVIGSLIQCFEWESMGGKCID 506
P FNP+RF S+GEGFKWMPFGAGRRRCPGE LGLRVVGL +GSLIQCF+WES+GG+C+D
Sbjct: 421 PGNFNPERFGSEGEGFKWMPFGAGRRRCPGEGLGLRVVGLAVGSLIQCFDWESVGGECVD 480
Query: 507 MSEGGGLTLPKALPLRTLCRPRSNATHLLSQI 539
M EGGGLTLPKALPL LCRPRSN HLL QI
Sbjct: 481 MREGGGLTLPKALPLHALCRPRSNVAHLLPQI 508
BLAST of Cmc07g0183581 vs. NCBI nr
Match:
XP_023535659.1 (isoflavone 2'-hydroxylase-like [Cucurbita pepo subsp. pepo])
HSP 1 Score: 852.8 bits (2202), Expect = 1.6e-243
Identity = 422/510 (82.75%), Postives = 454/510 (89.02%), Query Frame = 0
Query: 31 MVISFLYLLILSILQIFTNHFLHRIRNLPPSPFLSLPIIGQLYLLKAPLHRTLSEISRRH 90
M I+FL LLIL IL IFTN FLHRIRNLPPSPF SLPIIGQLYLLK PLHRTLS+ISRRH
Sbjct: 1 MAITFLCLLILLILHIFTNQFLHRIRNLPPSPFFSLPIIGQLYLLKRPLHRTLSDISRRH 60
Query: 91 GPILFFQFGFRPVLVVSSPSAAEDCLSKNDVIFANRPRLVSGKYLGYNYTSLLWAPYGEH 150
GPILFF FGFRPVLVVSSPSAAE+CLSKND++FANRP L+ GKYLGYNYTSLLWAPYGEH
Sbjct: 61 GPILFFWFGFRPVLVVSSPSAAEECLSKNDIVFANRPHLIGGKYLGYNYTSLLWAPYGEH 120
Query: 151 WRNLRRISSLEILSSHRLQTLSSIRVDEVRKLIRRLYNAENDVVDMRTEFFQLMFNIMMR 210
WRNLRR+SSLEI S+HRLQTLSSIRVDEVR LIRRLYN ENDVVDMRTEFF+LMFNIMMR
Sbjct: 121 WRNLRRVSSLEIFSTHRLQTLSSIRVDEVRTLIRRLYNTENDVVDMRTEFFELMFNIMMR 180
Query: 211 MIAGKRYYGEAVVADSEEGRRFQEIQEETFRLSGKTNLGDFLRVVKWVGLSKGIENRLRE 270
MIAGKRYYGE A+SEE RF+EIQEETFRLS KTNLGDFLR+VKW GLSKG+ENR++E
Sbjct: 181 MIAGKRYYGED-AAESEEAERFREIQEETFRLSSKTNLGDFLRIVKWAGLSKGLENRMKE 240
Query: 271 LQIRRDDWMQSLIEEHRKKMDNAHSSSSSSIQGDAKKTMIEVLLSLQQMEPQYYKDEYIR 330
LQ +RD WMQSLIEEHRK+ + DAKKTMIEVLLSLQQ EP+YYKDEYIR
Sbjct: 241 LQSKRDAWMQSLIEEHRKQS-----------KKDAKKTMIEVLLSLQQKEPRYYKDEYIR 300
Query: 331 GLMLVLLLAGTEGSINTMEWLLSLLLNHPHSLRRAQMEIDNVVGKTNRLLEESDLTHLPY 390
GLMLVLLLAGTEGSINTMEWLLSLLLN+P L+RAQ+EID+VVG++NR+LEESDL HLPY
Sbjct: 301 GLMLVLLLAGTEGSINTMEWLLSLLLNNPECLQRAQLEIDDVVGRSNRILEESDLAHLPY 360
Query: 391 LRSLIHETLRMYPPGPLLIPHESSQDCHVGGFHVPAGTMLFVNVWAIQNDPTVWVEPEKF 450
LRSLIHETLRMYPPGPLLIPHESS DCHVGGF VPAGT LFVNVWAIQNDPTVWV+PEKF
Sbjct: 361 LRSLIHETLRMYPPGPLLIPHESSADCHVGGFRVPAGTTLFVNVWAIQNDPTVWVDPEKF 420
Query: 451 NPDRFKSDGEG--FKWMPFGAGRRRCPGESLGLRVVGLVIGSLIQCFEWESMGGKCIDMS 510
NP RF S+GEG FKWMPFGAGRRRCPGE LGLRVVGL +GSLIQCFEWES+GG+CIDM
Sbjct: 421 NPGRFGSEGEGWFFKWMPFGAGRRRCPGEGLGLRVVGLAVGSLIQCFEWESVGGECIDMR 480
Query: 511 EGGGLTLPKALPLRTLCRPRSNATHLLSQI 539
EG GLTLPKA+PL LCRPRS HLL QI
Sbjct: 481 EGVGLTLPKAVPLHALCRPRSKVAHLLPQI 498
BLAST of Cmc07g0183581 vs. NCBI nr
Match:
KAG7024285.1 (Isoflavone 2'-hydroxylase, partial [Cucurbita argyrosperma subsp. argyrosperma])
HSP 1 Score: 852.0 bits (2200), Expect = 2.7e-243
Identity = 423/510 (82.94%), Postives = 452/510 (88.63%), Query Frame = 0
Query: 31 MVISFLYLLILSILQIFTNHFLHRIRNLPPSPFLSLPIIGQLYLLKAPLHRTLSEISRRH 90
M I FL LLIL IL IFTN FLHRIRNLPPSPF SLPIIGQLYLLK PLHRTLS+ISRRH
Sbjct: 1 MAIIFLCLLILLILHIFTNQFLHRIRNLPPSPFFSLPIIGQLYLLKRPLHRTLSDISRRH 60
Query: 91 GPILFFQFGFRPVLVVSSPSAAEDCLSKNDVIFANRPRLVSGKYLGYNYTSLLWAPYGEH 150
GPILFF FGFRPVLVVSSPSAAE+CLSKND++FANRP L+ GKYLGYNYTSLLWAPYGEH
Sbjct: 61 GPILFFWFGFRPVLVVSSPSAAEECLSKNDIVFANRPHLIGGKYLGYNYTSLLWAPYGEH 120
Query: 151 WRNLRRISSLEILSSHRLQTLSSIRVDEVRKLIRRLYNAENDVVDMRTEFFQLMFNIMMR 210
WRNLRRISSLEI S+HRLQTLSSIRVDEVR LIRRLYN +NDVVDMRTEFF+LMFNIMMR
Sbjct: 121 WRNLRRISSLEIFSTHRLQTLSSIRVDEVRTLIRRLYNTKNDVVDMRTEFFELMFNIMMR 180
Query: 211 MIAGKRYYGEAVVADSEEGRRFQEIQEETFRLSGKTNLGDFLRVVKWVGLSKGIENRLRE 270
MIAGKRYYGE A+SEE RF+EIQEETFRLS KTNLGDFLR+VKW GLSKG+ENR++E
Sbjct: 181 MIAGKRYYGED-AAESEEAERFREIQEETFRLSSKTNLGDFLRIVKWAGLSKGLENRMKE 240
Query: 271 LQIRRDDWMQSLIEEHRKKMDNAHSSSSSSIQGDAKKTMIEVLLSLQQMEPQYYKDEYIR 330
LQ +RD WMQSLIEEHRK+ + DAKKTMIEVLLSLQQ EP YYKDEYIR
Sbjct: 241 LQSKRDAWMQSLIEEHRKQSNK-----------DAKKTMIEVLLSLQQKEPHYYKDEYIR 300
Query: 331 GLMLVLLLAGTEGSINTMEWLLSLLLNHPHSLRRAQMEIDNVVGKTNRLLEESDLTHLPY 390
GLMLVLLLAGTEGSINTMEWLLSLLLN+P L+RAQ EID+VVG++NR+LEESDL HLPY
Sbjct: 301 GLMLVLLLAGTEGSINTMEWLLSLLLNNPECLQRAQTEIDDVVGRSNRILEESDLAHLPY 360
Query: 391 LRSLIHETLRMYPPGPLLIPHESSQDCHVGGFHVPAGTMLFVNVWAIQNDPTVWVEPEKF 450
LRSLIHETLRMYPPGPLLIPHESS+DCHVGGF VPAGT LFVNVWAIQNDPTVWVEPEKF
Sbjct: 361 LRSLIHETLRMYPPGPLLIPHESSEDCHVGGFRVPAGTTLFVNVWAIQNDPTVWVEPEKF 420
Query: 451 NPDRFKSDGEG--FKWMPFGAGRRRCPGESLGLRVVGLVIGSLIQCFEWESMGGKCIDMS 510
NP RF S+GEG FKWMPFGAGRRRCPGE LGLRVVGL +GSLIQCFEWES+GG+CIDM
Sbjct: 421 NPGRFGSEGEGWFFKWMPFGAGRRRCPGEGLGLRVVGLAVGSLIQCFEWESVGGECIDMR 480
Query: 511 EGGGLTLPKALPLRTLCRPRSNATHLLSQI 539
EG GLTLPKA+PL LCRPRS HLL QI
Sbjct: 481 EGVGLTLPKAVPLHALCRPRSKVAHLLPQI 498
BLAST of Cmc07g0183581 vs. ExPASy Swiss-Prot
Match:
W8JMU7 (Cytochrome P450 81Q32 OS=Catharanthus roseus OX=4058 GN=CYP81Q32 PE=2 SV=1)
HSP 1 Score: 474.9 bits (1221), Expect = 1.2e-132
Identity = 245/514 (47.67%), Postives = 350/514 (68.09%), Query Frame = 0
Query: 29 NSMVISFLYLLILSI-LQIFTNHFLHRIRNLPPSPFLSLPIIGQLYLLKAPLHRTLSEIS 88
++++ +FL +++LSI L++F R RNLPPSP L+LP+IG L+L+ LHR+L ++S
Sbjct: 4 STLLYTFLAVVLLSISLKLFP--VSRRRRNLPPSPGLALPVIGHLHLIGKLLHRSLYDLS 63
Query: 89 RRHGPILFFQFGFRPVLVVSSPSAAEDCLSKNDVIFANRPRLVSGKYLGYNYTSLLWAPY 148
+++G + Q G R VLVVSSP+AAE+C +KND++FANRP + GKY+GYNYT+++ +PY
Sbjct: 64 KKYGSVFSLQLGNRLVLVVSSPAAAEECFTKNDIVFANRPLFILGKYIGYNYTTMVGSPY 123
Query: 149 GEHWRNLRRISSLEILSSHRLQTLSSIRVDEVRKLIRRLYNAEND---VVDMRTEFFQLM 208
GEHWRNLRR++++EI S+ L SIR DEV++L+ LY + V+M+++ +L
Sbjct: 124 GEHWRNLRRLAAVEIFSAGSLNRFLSIREDEVKQLLLSLYQSSGQDFGKVEMKSKLSELS 183
Query: 209 FNIMMRMIAGKRYYGEAVVADSEEGRRFQEIQEETFRLSGKTNLGDFLRVVKWVGLSKGI 268
FN+ MRM+AGKRY+G+ V DS+E + F+ + E F +G +N GDF+ ++W+ K
Sbjct: 184 FNVTMRMVAGKRYFGQDV--DSDEAKLFRALIGEVFEHAGASNPGDFVPFLRWIDF-KNY 243
Query: 269 ENRLRELQIRRDDWMQSLIEEHRKKMDNAHSSSSSSIQGDAKKTMIEVLLSLQQMEPQYY 328
E ++ ++ D ++Q LI E R +N TMI+ LLSLQ+ +P+YY
Sbjct: 244 EKKVSKISQEMDAFLQRLIHESRINKNNV--------------TMIDHLLSLQESQPEYY 303
Query: 329 KDEYIRGLMLVLLLAGTEGSINTMEWLLSLLLNHPHSLRRAQMEIDNVVGKTNRLLEESD 388
D+ I+G+++VLLLAGT+ S T+EW +SLLLNHP +L +A+ EI+ VG +NRL+EE D
Sbjct: 304 TDQIIKGIIMVLLLAGTDTSAVTVEWAMSLLLNHPETLEKARTEIETQVG-SNRLIEEQD 363
Query: 389 LTHLPYLRSLIHETLRMYPPGPLLIPHESSQDCHVGGFHVPAGTMLFVNVWAIQNDPTVW 448
L L YL ++I ET R+ P P+L+PHESS DC V G+ VP GT+L VN WAI DP W
Sbjct: 364 LPKLTYLHNIISETFRLCPAAPMLVPHESSDDCKVQGYDVPKGTILLVNAWAIHRDPEFW 423
Query: 449 VEPEKFNPDRFKS-DGEGFKWMPFGAGRRRCPGESLGLRVVGLVIGSLIQCFEWESMGGK 508
EP F P+R + E K MPFG GRR CPG L RVVGL +G+LIQCFEW+ +G
Sbjct: 424 DEPTLFKPERHGGVELEPSKLMPFGMGRRSCPGSGLAQRVVGLTLGALIQCFEWKRIGEA 483
Query: 509 CIDMSEGGGLTLPKALPLRTLCRPRSNATHLLSQ 538
IDM+EG GLT+PKA PL LC+PR+ ++S+
Sbjct: 484 KIDMAEGSGLTMPKAQPLEALCKPRNILHKVVSE 497
BLAST of Cmc07g0183581 vs. ExPASy Swiss-Prot
Match:
Q6WNQ8 (Cytochrome P450 81E8 OS=Medicago truncatula OX=3880 GN=CYP81E8 PE=2 SV=1)
HSP 1 Score: 447.2 bits (1149), Expect = 2.6e-124
Identity = 231/504 (45.83%), Postives = 337/504 (66.87%), Query Frame = 0
Query: 30 SMVISFLYLLILSILQIFTNHFLHRIRNLPPSPFLSLPIIGQLYLLKAPLHRTLSEISRR 89
S++IS +L+I L++F N + +NLPP P LPIIG L+ LK PLH T +S++
Sbjct: 7 SLIISLFFLII--TLKVFFN-TSRKFKNLPPGP-QCLPIIGNLHQLKQPLHHTFHTLSQK 66
Query: 90 HGPILFFQFGFRPVLVVSSPSAAEDCLSKNDVIFANRPRLVSGKYLGYNYTSLLWAPYGE 149
+G I FG R V+VVSS + A++C +KND++ ANRP ++GKY+GYN T++ +PYG+
Sbjct: 67 YGQIFSLWFGSRLVVVVSSLTIAQECFTKNDIVLANRPHFLTGKYIGYNNTTVAQSPYGD 126
Query: 150 HWRNLRRISSLEILSSHRLQTLSSIRVDEVRKLIRRL----YNAENDVVDMRTEFFQLMF 209
HWRNLRRI S+EILSSHRL + IR DE+ +LI++L YN + V++R F ++ F
Sbjct: 127 HWRNLRRILSIEILSSHRLNSFLEIRRDEIMRLIQKLAQKSYNGFTE-VELRPMFSEMTF 186
Query: 210 NIMMRMIAGKRYYG-EAVVADSEEGRRFQEIQEETFRLSGKTNLGDFLRVVKWVGLSKGI 269
N +MRM++GKRYYG + V+D EE R F+ I +E L G N+GDFL ++W G+
Sbjct: 187 NTIMRMVSGKRYYGNDCDVSDVEEARLFRGIIKEVVSLGGANNVGDFLGFLRWFDFD-GL 246
Query: 270 ENRLRELQIRRDDWMQSLIEEHRKKMDNAHSSSSSSIQGDAKKTMIEVLLSLQQMEPQYY 329
E RL+++ R D ++Q LI+EHR N++ TMI+ LL+ QQ +P+YY
Sbjct: 247 EKRLKKISKRTDAFLQGLIDEHRFGKRNSN-------------TMIDHLLTQQQSQPEYY 306
Query: 330 KDEYIRGLMLVLLLAGTEGSINTMEWLLSLLLNHPHSLRRAQMEIDNVVGKTNRLLEESD 389
D+ I+GLM+V+LLAGT+ S T+EW +S LLNHP +++A+ E+D +G +R ++E D
Sbjct: 307 TDQIIKGLMVVMLLAGTDTSSVTIEWAMSNLLNHPEIMKKAKNELDTHIGH-DRQVDEHD 366
Query: 390 LTHLPYLRSLIHETLRMYPPGPLLIPHESSQDCHVGGFHVPAGTMLFVNVWAIQNDPTVW 449
++ LPYL+S+++ETLR++ PLL+PH SS+D +GG+++P T+L VN W I DP +W
Sbjct: 367 ISKLPYLQSIVYETLRLHAAAPLLVPHLSSEDFSLGGYNIPQNTILMVNAWVIHRDPNLW 426
Query: 450 VEPEKFNPDRFKSDGEGFKWMPFGAGRRRCPGESLGLRVVGLVIGSLIQCFEWESMGGKC 509
+P F P+RF+ +GE K + FG GRR CPGE+L R GL +G LIQCFEW+ +G +
Sbjct: 427 SDPTCFKPERFEKEGEVNKLLSFGLGRRACPGENLSQRTEGLTLGLLIQCFEWKRIGEEK 486
Query: 510 IDMSEGGGLTLPKALPLRTLCRPR 529
IDM E G+T K L +C+ R
Sbjct: 487 IDMVEAKGITAGKKTSLNAMCKVR 490
BLAST of Cmc07g0183581 vs. ExPASy Swiss-Prot
Match:
Q6WNQ9 (Isoflavone 3'-hydroxylase (Fragment) OS=Medicago truncatula OX=3880 GN=CYP81E9 PE=1 SV=1)
HSP 1 Score: 443.0 bits (1138), Expect = 4.9e-123
Identity = 232/505 (45.94%), Postives = 333/505 (65.94%), Query Frame = 0
Query: 32 VISFLYLLILSILQIFTNHFLHRIRNLPPSPFLSLPIIGQLYLLKAPLHRTLSEISRRHG 91
++S +++ + IL T+ R++NLPP P ++PIIG L+ LK PLHRT + +S+ +G
Sbjct: 7 LLSLSFIITIKILLKITS---RRLKNLPPGP-PTIPIIGNLHHLKHPLHRTFTTLSQTYG 66
Query: 92 PILFFQFGFRPVLVVSSPSAAEDCLSKNDVIFANRPRLVSGKYLGYNYTSLLWAPYGEHW 151
I FG R V+VVSSPS A +C +KND+I ANRPR ++GKY+ YNYT+L A YG+HW
Sbjct: 67 DIFSLWFGSRLVVVVSSPSLAHECFTKNDIILANRPRFLTGKYIFYNYTTLGSASYGDHW 126
Query: 152 RNLRRISSLEILSSHRLQTLSSIRVDEVRKLIRRLYNAENDV---------VDMRTEFFQ 211
RNLRRI+++++LS++RL + +R DE +LI++L DV V++R +
Sbjct: 127 RNLRRITTIDVLSNNRLNSFLGVRRDETNRLIQKLL---KDVVSEGFGFTKVELRPRLTE 186
Query: 212 LMFNIMMRMIAGKRYYG-EAVVADSEEGRRFQEIQEETFRLSGKTNLGDFLRVVKWVGLS 271
+ FN MMRMI+GKRYYG + V+D EE ++F+EI E L G N GDFL +++ V L
Sbjct: 187 MTFNAMMRMISGKRYYGDDGDVSDVEEAKQFREIISEMMSLLGANNKGDFLPLLRVVDLD 246
Query: 272 KGIENRLRELQIRRDDWMQSLIEEHRKKMDNAHSSSSSSIQGDAKKTMIEVLLSLQQMEP 331
+E R + + R + +++ LIEEHR+ N HS TMI+ LL L + +P
Sbjct: 247 -NLEKRCKRIAKRSNAFLEGLIEEHRR--GNIHSDGG---------TMIDHLLKLSESQP 306
Query: 332 QYYKDEYIRGLMLVLLLAGTEGSINTMEWLLSLLLNHPHSLRRAQMEIDNVVGKTNRLLE 391
+YY D I+GL+ +LLAGT+ S T+EW++S LLNHP L++A+ E+D +GK N+L++
Sbjct: 307 EYYSDHLIKGLIQGMLLAGTDTSAVTIEWVMSELLNHPEVLKKAKEELDTQIGK-NKLVD 366
Query: 392 ESDLTHLPYLRSLIHETLRMYPPGPLLIPHESSQDCHVGGFHVPAGTMLFVNVWAIQNDP 451
E DL+ LPYL+++I ETLR++PP PLL+PH SS+DC +G F+VP T++ NVW I DP
Sbjct: 367 EQDLSKLPYLQNIISETLRLHPPAPLLLPHYSSEDCTIGEFNVPKDTIILTNVWGIHRDP 426
Query: 452 TVWVEPEKFNPDRFKSDGEGFKWMPFGAGRRRCPGESLGLRVVGLVIGSLIQCFEWESMG 511
W + F P+RF+ + E K M FG GRR CPG SL R VG +G LIQCFEWE
Sbjct: 427 KHWNDALSFKPERFEKEEEVNKVMAFGLGRRACPGLSLAQRTVGFTVGLLIQCFEWERES 486
Query: 512 GKCIDMSEGGGLTLPKALPLRTLCR 527
+ +DM EG G+T+P +PLR +C+
Sbjct: 487 EEKLDMMEGKGITMPMKIPLRAMCK 491
BLAST of Cmc07g0183581 vs. ExPASy Swiss-Prot
Match:
P93147 (Isoflavone 2'-hydroxylase OS=Glycyrrhiza echinata OX=46348 GN=CYP81E1 PE=1 SV=2)
HSP 1 Score: 437.6 bits (1124), Expect = 2.1e-121
Identity = 221/499 (44.29%), Postives = 328/499 (65.73%), Query Frame = 0
Query: 34 SFLYLLILSILQIFTNHFLHRIRNLPPSPFLSLPIIGQLYLLKAPLHRTLSEISRRHGPI 93
S YL + I I + +NLPP P SLPIIG L+ LK PLHRT +S ++G +
Sbjct: 10 SVFYLALFFIFNIVIR--ARKFKNLPPGP-PSLPIIGNLHHLKRPLHRTFKGLSEKYGHV 69
Query: 94 LFFQFGFRPVLVVSSPSAAEDCLSKNDVIFANRPRLVSGKYLGYNYTSLLWAPYGEHWRN 153
FG R V+VVSS S + C +KNDV+ ANRPR +SGKY+ YNYT+L YGEHWRN
Sbjct: 70 FSLWFGSRLVVVVSSASEFQQCFTKNDVVLANRPRFLSGKYIFYNYTTLGSTSYGEHWRN 129
Query: 154 LRRISSLEILSSHRLQTLSSIRVDEVRKLIRRLYNAEND---VVDMRTEFFQLMFNIMMR 213
LRRI++L++LS+HR+ + S IR DE ++LI RL + + +++ + + + FN +MR
Sbjct: 130 LRRITALDVLSNHRINSFSGIRRDETQRLITRLADDSSTNFAEMELSSRLYDMTFNNIMR 189
Query: 214 MIAGKRYYGE-AVVADSEEGRRFQEIQEETFRLSGKTNLGDFLRVVKWVGLSKGIENRLR 273
MI+GKRYYGE +D +E +F+++ E +LSG N DF+ +++++ + +E RL+
Sbjct: 190 MISGKRYYGEDCDTSDLQEASQFRDMVSELLQLSGANNKTDFMPLLRFLDF-ENLEKRLK 249
Query: 274 ELQIRRDDWMQSLIEEHRKKMDNAHSSSSSSIQGDAKKTMIEVLLSLQQMEPQYYKDEYI 333
++ + D +++ LIEEHR K + A+ TMI+ LL+LQ +P+YY D+ I
Sbjct: 250 DISGKTDAFLRGLIEEHRTKKERAN-------------TMIDHLLNLQDSQPEYYTDQII 309
Query: 334 RGLMLVLLLAGTEGSINTMEWLLSLLLNHPHSLRRAQMEIDNVVGKTNRLLEESDLTHLP 393
+GL L +LLAGT+ S T+EW +S LLNHP L++ + E+D VG+ +RL++ESDL L
Sbjct: 310 KGLALAMLLAGTDSSAVTLEWSMSNLLNHPEVLKKVKDELDTHVGQ-DRLVDESDLPKLT 369
Query: 394 YLRSLIHETLRMYPPGPLLIPHESSQDCHVGGFHVPAGTMLFVNVWAIQNDPTVWVEPEK 453
YL+++I+ETLR+Y P PLL+PH +S +C++GG+ VP T++ +N WAI DP +W E
Sbjct: 370 YLKNVINETLRLYTPAPLLLPHSTSDECNIGGYKVPQDTIVLINAWAIHRDPELWTEATT 429
Query: 454 FNPDRFKSDGEGFKWMPFGAGRRRCPGESLGLRVVGLVIGSLIQCFEWESMGGKCIDMSE 513
F P+RF+ GE K + FG GRR CPGE L +R + + + LIQCF+W+ + G ID++E
Sbjct: 430 FKPERFEKKGELEKLIAFGMGRRACPGEGLAIRAISMTLALLIQCFDWKLINGDKIDLAE 489
Query: 514 GGGLTLPKALPLRTLCRPR 529
G TL K +PL+ +C+ R
Sbjct: 490 RDGFTLTKLVPLKAMCKSR 490
BLAST of Cmc07g0183581 vs. ExPASy Swiss-Prot
Match:
Q9FG65 (Cytochrome P450 81D1 OS=Arabidopsis thaliana OX=3702 GN=CYP81D1 PE=2 SV=1)
HSP 1 Score: 424.9 bits (1091), Expect = 1.4e-117
Identity = 225/503 (44.73%), Postives = 322/503 (64.02%), Query Frame = 0
Query: 36 LYLLILSILQIFTNHFLH-RIRNLPPSPFLSLPIIGQLYLLKAPLHRTLSEISRR----- 95
LY + I I + FL + +NLPPSP LPIIG L LLK P+HRTL S
Sbjct: 10 LYSIFSLIFLIISFKFLKPKKQNLPPSPPGWLPIIGHLRLLKPPIHRTLRSFSETLDHND 69
Query: 96 HGPILFFQFGFRPVLVVSSPS-AAEDCLSKNDVIFANRPRLVSGKYLGYNYTSLLWAPYG 155
G ++ + G R V VVSS AAE+C KNDV+ ANRP+++ GK++GYN T+++ APYG
Sbjct: 70 GGGVMSLRLGSRLVYVVSSHKVAAEECFGKNDVVLANRPQVIIGKHVGYNNTNMIAAPYG 129
Query: 156 EHWRNLRRISSLEILSSHRLQTLSSIRVDEVRKLI---RRLYNAENDVVDMRTEFFQLMF 215
+HWRNLRR+ ++EI S+HRL +R DEVR+LI RL + VV+++ L F
Sbjct: 130 DHWRNLRRLCTIEIFSTHRLNCFLYVRTDEVRRLISRLSRLAGTKKTVVELKPMLMDLTF 189
Query: 216 NIMMRMIAGKRYYGEAVVADSEEGRRFQEIQEETFRLSGKTNLGDFLRVVKWVGLSKGIE 275
N +MRM+ GKRYYGE D EE +R +++ + + N D++ +++ L E
Sbjct: 190 NNIMRMMTGKRYYGEE-TTDEEEAKRVRKLVADVGANTSSGNAVDYVPILR---LFSSYE 249
Query: 276 NRLRELQIRRDDWMQSLIEEHRKKMDNAHSSSSSSIQGDAKKTMIEVLLSLQQMEPQYYK 335
NR+++L D ++Q LI++ R Q + TMI+ LL LQ+ + +YY
Sbjct: 250 NRVKKLGEETDKFLQGLIDDKRG-------------QQETGTTMIDHLLVLQKSDIEYYT 309
Query: 336 DEYIRGLMLVLLLAGTEGSINTMEWLLSLLLNHPHSLRRAQMEIDNVVGKTNRLLEESDL 395
D+ I+G++L++++AGT S T+EW LS LLNHP + +A+ EIDN VG +RL+EE+DL
Sbjct: 310 DQIIKGIILIMVIAGTNTSAVTLEWALSNLLNHPDVISKARDEIDNRVG-LDRLIEEADL 369
Query: 396 THLPYLRSLIHETLRMYPPGPLLIPHESSQDCHVGGFHVPAGTMLFVNVWAIQNDPTVWV 455
+ LPYL++++ ETLR++P PLL+PH +S+DC +G + +P GT L VN WAI DP W
Sbjct: 370 SELPYLKNIVLETLRLHPATPLLVPHMASEDCKIGSYDMPRGTTLLVNAWAIHRDPNTWD 429
Query: 456 EPEKFNPDRFKSDGEGFKWMPFGAGRRRCPGESLGLRVVGLVIGSLIQCFEWESMGGKCI 515
+P+ F P+RF+ + E K + FG GRR CPG L R+VGL +GSLIQCFEWE +G +
Sbjct: 430 DPDSFKPERFEKEEEAQKLLAFGLGRRACPGSGLAQRIVGLALGSLIQCFEWERVGNVEV 489
Query: 516 DMSEGGGLTLPKALPLRTLCRPR 529
DM EG G T+PKA+PL+ +C+ R
Sbjct: 490 DMKEGVGNTVPKAIPLKAICKAR 494
BLAST of Cmc07g0183581 vs. ExPASy TrEMBL
Match:
A0A1S3BVF3 (cytochrome P450 81E8-like OS=Cucumis melo OX=3656 GN=LOC103493585 PE=3 SV=1)
HSP 1 Score: 1078.9 bits (2789), Expect = 0.0e+00
Identity = 534/538 (99.26%), Postives = 535/538 (99.44%), Query Frame = 0
Query: 1 MGFCFWFCFSQPRKLSFLLRMEDGSWMVNSMVISFLYLLILSILQIFTNHFLHRIRNLPP 60
MGFCFWFCFSQPRKLSFLLRMEDGSWMVNSMVISFLYLLIL ILQIFTNHFLHRIRNLPP
Sbjct: 1 MGFCFWFCFSQPRKLSFLLRMEDGSWMVNSMVISFLYLLILFILQIFTNHFLHRIRNLPP 60
Query: 61 SPFLSLPIIGQLYLLKAPLHRTLSEISRRHGPILFFQFGFRPVLVVSSPSAAEDCLSKND 120
SPFLSLPIIGQLYLLKAPLHRTLSEISRRHGPILFFQFGFRPVLVVSSPSA EDCLSKND
Sbjct: 61 SPFLSLPIIGQLYLLKAPLHRTLSEISRRHGPILFFQFGFRPVLVVSSPSAVEDCLSKND 120
Query: 121 VIFANRPRLVSGKYLGYNYTSLLWAPYGEHWRNLRRISSLEILSSHRLQTLSSIRVDEVR 180
VIFANRPRLVSGKYLGYNYTSLLWAPYGEHWRNLRRISSLEILSSHRLQTLSSIRVDEVR
Sbjct: 121 VIFANRPRLVSGKYLGYNYTSLLWAPYGEHWRNLRRISSLEILSSHRLQTLSSIRVDEVR 180
Query: 181 KLIRRLYNAENDVVDMRTEFFQLMFNIMMRMIAGKRYYGEAVVADSEEGRRFQEIQEETF 240
KLIRRLYNAENDVVDMRTEFF+LMFNIMMRMIAGKRYYGEAVVADSEEGRRFQEIQEETF
Sbjct: 181 KLIRRLYNAENDVVDMRTEFFELMFNIMMRMIAGKRYYGEAVVADSEEGRRFQEIQEETF 240
Query: 241 RLSGKTNLGDFLRVVKWVGLSKGIENRLRELQIRRDDWMQSLIEEHRKKMDNAHSSSSSS 300
RLSGKTNLGDFLRVVKWVGLSKGIENRLRELQIRRDDWMQSLIEEHRKKMDNAHSSSSSS
Sbjct: 241 RLSGKTNLGDFLRVVKWVGLSKGIENRLRELQIRRDDWMQSLIEEHRKKMDNAHSSSSSS 300
Query: 301 IQGDAKKTMIEVLLSLQQMEPQYYKDEYIRGLMLVLLLAGTEGSINTMEWLLSLLLNHPH 360
IQGDAKKTMIEVLLSLQQMEPQ YKDEYIRGLMLVLLLAGTEGSINTMEWLLSLLLNHPH
Sbjct: 301 IQGDAKKTMIEVLLSLQQMEPQNYKDEYIRGLMLVLLLAGTEGSINTMEWLLSLLLNHPH 360
Query: 361 SLRRAQMEIDNVVGKTNRLLEESDLTHLPYLRSLIHETLRMYPPGPLLIPHESSQDCHVG 420
SLRRAQMEIDNVVGKTNRLLEESDLTHLPYLRSLIHETLRMYPPGPLLIPHESSQDCHVG
Sbjct: 361 SLRRAQMEIDNVVGKTNRLLEESDLTHLPYLRSLIHETLRMYPPGPLLIPHESSQDCHVG 420
Query: 421 GFHVPAGTMLFVNVWAIQNDPTVWVEPEKFNPDRFKSDGEGFKWMPFGAGRRRCPGESLG 480
GFHVPAGTMLFVNVWAIQNDPTVWVEPEKFNPDRFKSDGEGFKWMPFGAGRRRCPGESLG
Sbjct: 421 GFHVPAGTMLFVNVWAIQNDPTVWVEPEKFNPDRFKSDGEGFKWMPFGAGRRRCPGESLG 480
Query: 481 LRVVGLVIGSLIQCFEWESMGGKCIDMSEGGGLTLPKALPLRTLCRPRSNATHLLSQI 539
LRVVGLVIGSLIQCFEWESMGGKCIDMSEGGGLTLPKALPLRTLCRPRSNATHLLSQI
Sbjct: 481 LRVVGLVIGSLIQCFEWESMGGKCIDMSEGGGLTLPKALPLRTLCRPRSNATHLLSQI 538
BLAST of Cmc07g0183581 vs. ExPASy TrEMBL
Match:
A0A0A0L0F1 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G642290 PE=3 SV=1)
HSP 1 Score: 952.6 bits (2461), Expect = 7.1e-274
Identity = 473/508 (93.11%), Postives = 489/508 (96.26%), Query Frame = 0
Query: 31 MVISFLYLLILSILQIFTNHFLHRIRNLPPSPFLSLPIIGQLYLLKAPLHRTLSEISRRH 90
MVI FL+LLIL +LQIFTNHFLHRIRNLPPSPFLSLPIIGQLYLLK PLHRTLSEISRRH
Sbjct: 1 MVIPFLHLLILFLLQIFTNHFLHRIRNLPPSPFLSLPIIGQLYLLKTPLHRTLSEISRRH 60
Query: 91 GPILFFQFGFRPVLVVSSPSAAEDCLSKNDVIFANRPRLVSGKYLGYNYTSLLWAPYGEH 150
GPI+FFQFGFR VLVVSSPSAAEDCLSKNDVIFANRPRLVSGKYLGYNYTSLLWAPYGEH
Sbjct: 61 GPIVFFQFGFRSVLVVSSPSAAEDCLSKNDVIFANRPRLVSGKYLGYNYTSLLWAPYGEH 120
Query: 151 WRNLRRISSLEILSSHRLQTLSSIRVDEVRKLIRRLYNAENDVVDMRTEFFQLMFNIMMR 210
WRNLRRISSLEILSSHRLQTLSSIRVDEVR LIRRLYNAENDVVDMRTEFF++MFNI MR
Sbjct: 121 WRNLRRISSLEILSSHRLQTLSSIRVDEVRTLIRRLYNAENDVVDMRTEFFEVMFNITMR 180
Query: 211 MIAGKRYYGEAVVADSEEGRRFQEIQEETFRLSGKTNLGDFLRVVKWVGLSKGIENRLRE 270
MIAGKRYYGE V A SEE RF+EIQEETFRLSGKTNLGDFLRVVKWVGLSKGIENRLRE
Sbjct: 181 MIAGKRYYGEDVAAYSEEAGRFREIQEETFRLSGKTNLGDFLRVVKWVGLSKGIENRLRE 240
Query: 271 LQIRRDDWMQSLIEEHRKKMDNAHSSSSSSIQGDAKKTMIEVLLSLQQMEPQYYKDEYIR 330
LQI+RDDWMQSLIEEHRKKM+NA+ SSSSSIQ DAKKTMIEVLLSLQQ EPQYYKDEYIR
Sbjct: 241 LQIKRDDWMQSLIEEHRKKMNNAY-SSSSSIQTDAKKTMIEVLLSLQQKEPQYYKDEYIR 300
Query: 331 GLMLVLLLAGTEGSINTMEWLLSLLLNHPHSLRRAQMEIDNVVGKTNRLLEESDLTHLPY 390
GLMLVLLLAGTEGSINTMEWLLSLLLNHPHSL+RAQMEID+VVG+TNRLLEESDLTHLPY
Sbjct: 301 GLMLVLLLAGTEGSINTMEWLLSLLLNHPHSLQRAQMEIDDVVGRTNRLLEESDLTHLPY 360
Query: 391 LRSLIHETLRMYPPGPLLIPHESSQDCHVGGFHVPAGTMLFVNVWAIQNDPTVWVEPEKF 450
LRSLIHETLRMYPPGPLLIPHESS+DCHVGGFHVPAGTMLFVNVWAIQNDPTVWVEP KF
Sbjct: 361 LRSLIHETLRMYPPGPLLIPHESSEDCHVGGFHVPAGTMLFVNVWAIQNDPTVWVEPRKF 420
Query: 451 NPDRFKSDGEGFKWMPFGAGRRRCPGESLGLRVVGLVIGSLIQCFEWESMGGKCIDMSEG 510
NPDRF DGEGFKWMPFGAGRRRCPGE LGLRV+GLV+GSLIQCFEWESM G+CIDMSEG
Sbjct: 421 NPDRFGGDGEGFKWMPFGAGRRRCPGEGLGLRVIGLVVGSLIQCFEWESMDGECIDMSEG 480
Query: 511 GGLTLPKALPLRTLCRPRSNATHLLSQI 539
GGLTLPKALPLRTLCRPRSNATHLLSQI
Sbjct: 481 GGLTLPKALPLRTLCRPRSNATHLLSQI 507
BLAST of Cmc07g0183581 vs. ExPASy TrEMBL
Match:
A0A6J1FED9 (isoflavone 2'-hydroxylase-like OS=Cucurbita moschata OX=3662 GN=LOC111443311 PE=3 SV=1)
HSP 1 Score: 850.1 bits (2195), Expect = 5.0e-243
Identity = 422/510 (82.75%), Postives = 452/510 (88.63%), Query Frame = 0
Query: 31 MVISFLYLLILSILQIFTNHFLHRIRNLPPSPFLSLPIIGQLYLLKAPLHRTLSEISRRH 90
M I FL LLIL IL IFTN FLHRIRNLPPSPF SLPIIGQLYLLK PLHRTLS+ISRRH
Sbjct: 1 MAIIFLCLLILLILHIFTNQFLHRIRNLPPSPFFSLPIIGQLYLLKRPLHRTLSDISRRH 60
Query: 91 GPILFFQFGFRPVLVVSSPSAAEDCLSKNDVIFANRPRLVSGKYLGYNYTSLLWAPYGEH 150
GPILFF FGFRPVLVVSSPSAAE+CLSKND++FANRP L+ GKYLGYNYTSLLWAPYGEH
Sbjct: 61 GPILFFWFGFRPVLVVSSPSAAEECLSKNDIVFANRPHLIGGKYLGYNYTSLLWAPYGEH 120
Query: 151 WRNLRRISSLEILSSHRLQTLSSIRVDEVRKLIRRLYNAENDVVDMRTEFFQLMFNIMMR 210
WRNLRRISSLEI S+HRLQTLSSIRVDEVR LIRRLYN +NDVVDMRTEFF+LMFNIMMR
Sbjct: 121 WRNLRRISSLEIFSTHRLQTLSSIRVDEVRTLIRRLYNTKNDVVDMRTEFFELMFNIMMR 180
Query: 211 MIAGKRYYGEAVVADSEEGRRFQEIQEETFRLSGKTNLGDFLRVVKWVGLSKGIENRLRE 270
MIAGKRYYGE A+SEE RF+EIQEETFRLS KTNLGDFLR+VKW GLSKG+ENR++E
Sbjct: 181 MIAGKRYYGED-AAESEEAERFREIQEETFRLSSKTNLGDFLRIVKWAGLSKGLENRMKE 240
Query: 271 LQIRRDDWMQSLIEEHRKKMDNAHSSSSSSIQGDAKKTMIEVLLSLQQMEPQYYKDEYIR 330
LQ +RD WMQSLIEEHRK+ + DAKKTMIEVLLSLQQ EP YYKDEYIR
Sbjct: 241 LQSKRDAWMQSLIEEHRKQS-----------KKDAKKTMIEVLLSLQQKEPHYYKDEYIR 300
Query: 331 GLMLVLLLAGTEGSINTMEWLLSLLLNHPHSLRRAQMEIDNVVGKTNRLLEESDLTHLPY 390
GLMLVLLLAGTEGSINTMEWLLSLLLN+P L+RAQ EID+VVG++NR+LEESDL HLPY
Sbjct: 301 GLMLVLLLAGTEGSINTMEWLLSLLLNNPECLQRAQTEIDDVVGRSNRILEESDLAHLPY 360
Query: 391 LRSLIHETLRMYPPGPLLIPHESSQDCHVGGFHVPAGTMLFVNVWAIQNDPTVWVEPEKF 450
LRSLIHETLRMYPPGPLLIPHESS+DCHVGGF VPAGT LFVNVWAIQNDPTVWV+PEKF
Sbjct: 361 LRSLIHETLRMYPPGPLLIPHESSEDCHVGGFRVPAGTTLFVNVWAIQNDPTVWVDPEKF 420
Query: 451 NPDRFKSDGEG--FKWMPFGAGRRRCPGESLGLRVVGLVIGSLIQCFEWESMGGKCIDMS 510
NP RF S+GEG FKWMPFGAGRRRCPGE LGLRVVGL +GSLIQCFEWES+GG+CIDM
Sbjct: 421 NPGRFGSEGEGWFFKWMPFGAGRRRCPGEGLGLRVVGLAVGSLIQCFEWESVGGECIDMR 480
Query: 511 EGGGLTLPKALPLRTLCRPRSNATHLLSQI 539
EG GLTLPKA+PL LCRPRS HLL QI
Sbjct: 481 EGVGLTLPKAVPLHALCRPRSKVAHLLPQI 498
BLAST of Cmc07g0183581 vs. ExPASy TrEMBL
Match:
A0A6J1INS3 (isoflavone 2'-hydroxylase-like OS=Cucurbita maxima OX=3661 GN=LOC111477207 PE=3 SV=1)
HSP 1 Score: 847.4 bits (2188), Expect = 3.2e-242
Identity = 421/510 (82.55%), Postives = 452/510 (88.63%), Query Frame = 0
Query: 31 MVISFLYLLILSILQIFTNHFLHRIRNLPPSPFLSLPIIGQLYLLKAPLHRTLSEISRRH 90
M I+FL LLIL IL IFTN FLHRIRNLPPSPF SLPIIGQLYLLK PLHRTL +ISRRH
Sbjct: 1 MAITFLCLLILFILHIFTNQFLHRIRNLPPSPFFSLPIIGQLYLLKRPLHRTLLDISRRH 60
Query: 91 GPILFFQFGFRPVLVVSSPSAAEDCLSKNDVIFANRPRLVSGKYLGYNYTSLLWAPYGEH 150
GPILFF FGFRPVLVVSSPSAAE+CLSKND++FANRP L+ GKYLGYNYTSLLWAPYGEH
Sbjct: 61 GPILFFWFGFRPVLVVSSPSAAEECLSKNDIVFANRPHLIGGKYLGYNYTSLLWAPYGEH 120
Query: 151 WRNLRRISSLEILSSHRLQTLSSIRVDEVRKLIRRLYNAENDVVDMRTEFFQLMFNIMMR 210
WRNLRRISSLEI S+HRLQTLSSIRVDEVR LIRRLYN +NDVVDM+TEFF+LMFNIMMR
Sbjct: 121 WRNLRRISSLEIFSTHRLQTLSSIRVDEVRTLIRRLYNTKNDVVDMKTEFFELMFNIMMR 180
Query: 211 MIAGKRYYGEAVVADSEEGRRFQEIQEETFRLSGKTNLGDFLRVVKWVGLSKGIENRLRE 270
MIAGKRYYGE VA+SEE RF+EIQEETFRLS KTNLGDFLR+VKW GLSKG+ENR++E
Sbjct: 181 MIAGKRYYGED-VAESEEAERFREIQEETFRLSSKTNLGDFLRMVKWAGLSKGLENRMKE 240
Query: 271 LQIRRDDWMQSLIEEHRKKMDNAHSSSSSSIQGDAKKTMIEVLLSLQQMEPQYYKDEYIR 330
LQ +RD WMQSLIEEHRK+ + DAKKTMIEVLLSLQQ EP YYKDEYIR
Sbjct: 241 LQSKRDAWMQSLIEEHRKQS-----------KKDAKKTMIEVLLSLQQKEPHYYKDEYIR 300
Query: 331 GLMLVLLLAGTEGSINTMEWLLSLLLNHPHSLRRAQMEIDNVVGKTNRLLEESDLTHLPY 390
GLMLVLLLAGTEGSINTMEWLLSLLLN+P L+RAQ EID+VVG++NR+LEESDL HLPY
Sbjct: 301 GLMLVLLLAGTEGSINTMEWLLSLLLNNPECLQRAQTEIDDVVGRSNRILEESDLAHLPY 360
Query: 391 LRSLIHETLRMYPPGPLLIPHESSQDCHVGGFHVPAGTMLFVNVWAIQNDPTVWVEPEKF 450
LRSLIHETLRMYPPGPLLIPHESS DCHVGGF VPAGT LFVNVWAIQNDPTVWV+PEKF
Sbjct: 361 LRSLIHETLRMYPPGPLLIPHESSADCHVGGFRVPAGTTLFVNVWAIQNDPTVWVDPEKF 420
Query: 451 NPDRFKSDGEG--FKWMPFGAGRRRCPGESLGLRVVGLVIGSLIQCFEWESMGGKCIDMS 510
NP RF S+GEG FKWMPFGAGRRRCPGE LGLRVVGL +GSLIQCFEWES+GG+CIDM
Sbjct: 421 NPGRFGSEGEGWFFKWMPFGAGRRRCPGEGLGLRVVGLAVGSLIQCFEWESVGGECIDMR 480
Query: 511 EGGGLTLPKALPLRTLCRPRSNATHLLSQI 539
EG GLTLPKA+PL LCRPRS HLL QI
Sbjct: 481 EGVGLTLPKAVPLHALCRPRSKVAHLLPQI 498
BLAST of Cmc07g0183581 vs. ExPASy TrEMBL
Match:
A0A6P4BCT8 (cytochrome P450 81E8-like OS=Ziziphus jujuba OX=326968 GN=LOC107433678 PE=3 SV=1)
HSP 1 Score: 651.4 bits (1679), Expect = 3.4e-183
Identity = 326/514 (63.42%), Postives = 405/514 (78.79%), Query Frame = 0
Query: 35 FLYLLILSILQIFTNHFLHRIRNLPPSPFLSLPIIGQLYLLKAPLHRTLSEISRRHGPIL 94
F Y+L+ S + +FT H H+I+NLPPSPF LP+IG LYLL+ PLHRTLS+++ R+GPIL
Sbjct: 6 FFYILLFSAVYVFTKHLFHKIQNLPPSPF-PLPVIGHLYLLERPLHRTLSKLAGRYGPIL 65
Query: 95 FFQFGFRPVLVVSSPSAAEDCLSKNDVIFANRPRLVSGKYLGYNYTSLLWAPYGEHWRNL 154
F + G RPVLVVSS SAAE+CL+KND+IFANRPRL+ GKYLGYN+TS+ WAPYG+HWRNL
Sbjct: 66 FLKLGSRPVLVVSSSSAAEECLAKNDIIFANRPRLMVGKYLGYNFTSVAWAPYGDHWRNL 125
Query: 155 RRISSLEILSSHRLQTLSSIRVDEVRKLIRRLYNAENDVVDMRTEFFQLMFNIMMRMIAG 214
RRISSLE+LSSHRLQ LS IR++EV+ L+RRL+ + VVDMR FF+L NIMMRMIAG
Sbjct: 126 RRISSLELLSSHRLQMLSGIRLEEVKLLLRRLFEKQGVVVDMRQAFFELTLNIMMRMIAG 185
Query: 215 KRYYGEAVVADSEEGRRFQEIQEETFRLSGKTNLGDFLRVVKWVGLSKGIENRLRELQIR 274
KRYYG+ VV D +E RF+EIQEET RLSGK+NLGDFL V+W+G SK +E L ELQ +
Sbjct: 186 KRYYGDEVVEDLKEAMRFREIQEETVRLSGKSNLGDFLPFVRWIGGSKRLEKSLMELQRK 245
Query: 275 RDDWMQSLIEEHRKKM-----DNAHSSSSSSIQGDAKKTMIEVLLSLQQMEPQYYKDEYI 334
RD ++Q LIEEHR+ D S S S + +KTM+EVLLSLQQMEP+YYKDE I
Sbjct: 246 RDGFLQDLIEEHRRTASRIANDCCSPSPSPSKSKERRKTMLEVLLSLQQMEPEYYKDEII 305
Query: 335 RGLMLVLLLAGTEGSINTMEWLLSLLLNHPHSLRRAQMEIDNVVGKTNRLLEESDLTHLP 394
RGLMLVLLL GT+ +INT++W LSLLLNHP L++AQ EIDN VG RL++ESD+ LP
Sbjct: 306 RGLMLVLLLGGTDTTINTLQWSLSLLLNHPEILKKAQDEIDNRVGH-GRLVDESDVAQLP 365
Query: 395 YLRSLIHETLRMYPPGPLLIPHESSQDCHVGGFHVPAGTMLFVNVWAIQNDPTVWVEPEK 454
YL+S+I+ETLRMYPP PLL+PHESS++ VGGF +P GT L VN+WAIQNDPT+WV+P
Sbjct: 366 YLKSIINETLRMYPPIPLLLPHESSEESTVGGFRIPRGTTLLVNIWAIQNDPTIWVDPTS 425
Query: 455 FNPDRF-KSDG---EGFKWMPFGAGRRRCPGESLGLRVVGLVIGSLIQCFEWESMG-GKC 514
F P+R+ +++G + + MPFG+GRR CPGE LG+RVVGLV+G +IQCF WE + K
Sbjct: 426 FKPERYIEAEGMRNDKLRLMPFGSGRRGCPGEGLGMRVVGLVLGLIIQCFNWERVSQEKM 485
Query: 515 IDMSEGGGLTLPKALPLRTLCRPRSNATHLLSQI 539
+DMSEG GLTLPK+ PL+ C PRS + L+SQI
Sbjct: 486 VDMSEGNGLTLPKSQPLQAKCWPRSTMSKLVSQI 517
BLAST of Cmc07g0183581 vs. TAIR 10
Match:
AT4G37340.1 (cytochrome P450, family 81, subfamily D, polypeptide 3 )
HSP 1 Score: 448.0 bits (1151), Expect = 1.1e-125
Identity = 233/514 (45.33%), Postives = 334/514 (64.98%), Query Frame = 0
Query: 30 SMVISFLYLLILSILQIFTNHFLHRIRNLPPSPFLSLPIIGQLYLLKAPLHRTLSEISRR 89
+++ +FL+ +S+ F + R NLPPSP +LP+IG L LLK PLHR +S
Sbjct: 5 TLIFTFLF---ISLSLTFIIGRIKRRPNLPPSPSWALPVIGHLRLLKPPLHRVFLSVSES 64
Query: 90 HG--PILFFQFGFRPVLVVSSPSAAEDCLSKNDVIFANRPRLVSGKYLGYNYTSLLWAPY 149
G PI+ + G R V VVSS S AE+C +KNDV+ ANR ++ K++ Y T+++ A Y
Sbjct: 65 LGDAPIISLRLGNRLVFVVSSHSLAEECFTKNDVVLANRFNSLASKHISYGCTTVVTASY 124
Query: 150 GEHWRNLRRISSLEILSSHRLQTLSSIRVDEVRKLI---RRLYNAENDVVDMRTEFFQLM 209
G+HWRNLRRI ++EI S+HRL + SSIR DE+ +LI R + E V+M++ F L
Sbjct: 125 GDHWRNLRRIGAVEIFSAHRLNSFSSIRRDEIHRLIACLSRNSSLEFTKVEMKSMFSNLT 184
Query: 210 FNIMMRMIAGKRYYGEAVVADSEEGRRFQEIQEETFRLSGKTNLGDFLRVVKWVGLSKGI 269
FN ++RM+AGK YYG+ D E +R +E+ E G N D+L ++ W+ G
Sbjct: 185 FNNIIRMLAGKCYYGDG-AEDDPEAKRVRELIAEGMGCFGAGNTADYLPILTWI---TGS 244
Query: 270 ENRLRELQIRRDDWMQSLIEEHRKKMDNAHSSSSSSIQGDAKKTMIEVLLSLQQMEPQYY 329
E R++++ R D+++Q L++E R+ + + TM++ LL LQ+ +P+YY
Sbjct: 245 EKRIKKIASRLDEFLQGLVDERREGKEK------------RQNTMVDHLLCLQETQPEYY 304
Query: 330 KDEYIRGLMLVLLLAGTEGSINTMEWLLSLLLNHPHSLRRAQMEIDNVVGKTNRLLEESD 389
D I+G+ML L+LAGT+ S T+EW LS LLNHP L +A+ EIDN VG NRL+EESD
Sbjct: 305 TDNIIKGIMLSLILAGTDTSAVTLEWTLSALLNHPQILSKARDEIDNKVG-LNRLVEESD 364
Query: 390 LTHLPYLRSLIHETLRMYPPGPLLIPHESSQDCHVGGFHVPAGTMLFVNVWAIQNDPTVW 449
L+HLPYL++++ E+LR+YP PLL+PH +S+DC VGG+H+P GTML N WAI DP +W
Sbjct: 365 LSHLPYLQNIVSESLRLYPASPLLVPHVASEDCKVGGYHMPRGTMLLTNAWAIHRDPKIW 424
Query: 450 VEPEKFNPDRFKSDGEGFKWMPFGAGRRRCPGESLGLRVVGLVIGSLIQCFEWESMGGKC 509
+P F P+RF+ +GE K + FG GRR CPG L R+ L IGSLIQCFEWE +G +
Sbjct: 425 DDPTSFKPERFEKEGEAQKLLGFGLGRRACPGSGLAQRLASLTIGSLIQCFEWERIGEEE 484
Query: 510 IDMSE-GGGLTLPKALPLRTLCRPRSNATHLLSQ 538
+DM+E GGG+ +PKA+PL +C+ R +L++
Sbjct: 485 VDMTEGGGGVIMPKAIPLVAMCKARPVVGKILNE 498
BLAST of Cmc07g0183581 vs. TAIR 10
Match:
AT4G37320.1 (cytochrome P450, family 81, subfamily D, polypeptide 5 )
HSP 1 Score: 447.6 bits (1150), Expect = 1.4e-125
Identity = 233/504 (46.23%), Postives = 333/504 (66.07%), Query Frame = 0
Query: 31 MVISFLYLLILSILQIFTNHFLHRIRNLPPSPFLSLPIIGQLYLLKAPLHRTLSEISRRH 90
+++SFL+L I SI + T +R NLPPSP LP+IG L+LLK P+HRT IS+
Sbjct: 7 LLLSFLFLTI-SIKLLLTKS--NRKPNLPPSPAYPLPVIGHLHLLKQPVHRTFHSISKSL 66
Query: 91 G--PILFFQFGFRPVLVVSSPSAAEDCLSKNDVIFANRPRLVSGKYLGYNYTSLLWAPYG 150
G PI + G R V V+SS S AE+C +KNDV+ ANRP ++ K++GYN+T+++ A YG
Sbjct: 67 GNAPIFHLRLGNRLVYVISSHSIAEECFTKNDVVLANRPDIIMAKHVGYNFTNMIAASYG 126
Query: 151 EHWRNLRRISSLEILSSHRLQTLSSIRVDEVRKLIRRLYNAEND---VVDMRTEFFQLMF 210
+HWRNLRRI+++EI SSHR+ T SSIR DE+R+LI L V++++ L F
Sbjct: 127 DHWRNLRRIAAVEIFSSHRISTFSSIRKDEIRRLITHLSRDSLHGFVEVELKSLLTNLAF 186
Query: 211 NIMMRMIAGKRYYGEAVVADSEEGRRFQEIQEETFRLSGKTNLGDFLRVVKWVGLSKGIE 270
N ++ M+AGKRYYG D++E + +E+ E +G NL D+L + WV E
Sbjct: 187 NNIIMMVAGKRYYGTG-TEDNDEAKLVRELIAEIMAGAGSGNLADYLPSINWV---TNFE 246
Query: 271 NRLRELQIRRDDWMQSLIEEHRKKMDNAHSSSSSSIQGDAKKTMIEVLLSLQQMEPQYYK 330
N+ + L R D +Q L++E R + + +T+I+ LLS Q+ EP+YY
Sbjct: 247 NQTKILGNRLDRVLQKLVDEKRAEKEKG-------------QTLIDHLLSFQETEPEYYT 306
Query: 331 DEYIRGLMLVLLLAGTEGSINTMEWLLSLLLNHPHSLRRAQMEIDNVVGKTNRLLEESDL 390
D I+G++L L+LAGT+ S T+EW +S LLNHP L +A+ EID+ +G ++RL+EESD+
Sbjct: 307 DVIIKGIILALVLAGTDTSSVTLEWAMSNLLNHPEILEKARAEIDDKIG-SDRLVEESDI 366
Query: 391 THLPYLRSLIHETLRMYPPGPLLIPHESSQDCHVGGFHVPAGTMLFVNVWAIQNDPTVWV 450
+L YL++++ ETLR+YP PLL+PH SS +C V G+ +P T+L NVWA+ DP +W
Sbjct: 367 VNLHYLQNIVSETLRLYPAVPLLLPHFSSDECKVAGYDMPRRTLLLTNVWAMHRDPGLWE 426
Query: 451 EPEKFNPDRFKSDGEGFKWMPFGAGRRRCPGESLGLRVVGLVIGSLIQCFEWESMGGKCI 510
EPE+F P+RF+ +GE K MPFG GRR CPG LG R+V L +G LIQ FEWE +G + +
Sbjct: 427 EPERFKPERFEKEGEARKLMPFGMGRRACPGAELGKRLVSLALGCLIQSFEWERVGAELV 486
Query: 511 DMSEGGGLTLPKALPLRTLCRPRS 530
DM+EG G+T+PKA PLR +C+ R+
Sbjct: 487 DMTEGEGITMPKATPLRAMCKARA 489
BLAST of Cmc07g0183581 vs. TAIR 10
Match:
AT4G37330.1 (cytochrome P450, family 81, subfamily D, polypeptide 4 )
HSP 1 Score: 443.7 bits (1140), Expect = 2.1e-124
Identity = 232/510 (45.49%), Postives = 330/510 (64.71%), Query Frame = 0
Query: 31 MVISFLYLLILSILQIFTNHFLHRIRNLPPSPFLSLPIIGQLYLLKAPLHRTLSEISRRH 90
+++SFL L L+I + + R NLPPSP +SLP+IG L+LLK PLHRT +S+
Sbjct: 7 LILSFLSLFTLAIFLLTRS---KRKLNLPPSPAISLPVIGHLHLLKPPLHRTFLSLSKSI 66
Query: 91 G--PILFFQFGFRPVLVVSSPSAAEDCLSKNDVIFANRPRLVSGKYLGYNYTSLLWAPYG 150
G P+ + G R V V+SS S AE+C +KNDV+ ANRP+ K+LGYN T LL A YG
Sbjct: 67 GNAPVFHLRLGNRLVYVISSRSIAEECFTKNDVVLANRPKFTISKHLGYNATYLLSASYG 126
Query: 151 EHWRNLRRISSLEILSSHRLQTLSSIRVDEVRKLIRRLYNAEND---VVDMRTEFFQLMF 210
+HWRNLRRI+++EI S+HRL + IR DE+R+LI L V+M+T L
Sbjct: 127 DHWRNLRRIAAVEIFSTHRLNSFLYIRKDEIRRLISHLSRDSLHGFVEVEMKTLLTNLAS 186
Query: 211 NIMMRMIAGKRYYGEAVVADSEEGRRFQEIQEETFRLSGKTNLGDFLRVVKWVGLSKGIE 270
N +RM+AGKRY+GE D+++ + + + E +G N D+L +++WV E
Sbjct: 187 NTTIRMLAGKRYFGE----DNDDAKLVKNLVSEAVTSAGAGNPIDYLSILRWV---SSYE 246
Query: 271 NRLRELQIRRDDWMQSLIEEHRKKMDNAHSSSSSSIQGDAKKTMIEVLLSLQQMEPQYYK 330
R++ L R D ++Q L++E R + + +TMI+ LL+LQ ++P YY
Sbjct: 247 KRIKNLGNRFDTFLQKLVDEKRAEKEKG-------------ETMIDHLLALQDIQPDYYT 306
Query: 331 DEYIRGLMLVLLLAGTEGSINTMEWLLSLLLNHPHSLRRAQMEIDNVVGKTNRLLEESDL 390
D I+G++L L++AGT+ S T+EW +S LLNHP L++A+MEID VG +RL++ESD+
Sbjct: 307 DVIIKGIILTLIIAGTDTSSVTLEWAMSNLLNHPEILKKARMEIDEKVG-LDRLVDESDI 366
Query: 391 THLPYLRSLIHETLRMYPPGPLLIPHESSQDCHVGGFHVPAGTMLFVNVWAIQNDPTVWV 450
+L YL+S++ ETLRMYP PLL+PH SS+DC VGG+ +P+GTM+ N WA+ DP VW
Sbjct: 367 VNLSYLQSIVLETLRMYPAVPLLLPHLSSEDCKVGGYDIPSGTMVLTNAWAMHRDPEVWE 426
Query: 451 EPEKFNPDRFKSDGEGFKWMPFGAGRRRCPGESLGLRVVGLVIGSLIQCFEWESMGGKCI 510
+PE F P+RF+ +GE K + FG GRR CPG L R++ +GSL+QCFEWE +G +
Sbjct: 427 DPEIFKPERFEKEGEAEKLISFGMGRRACPGAGLAHRLINQALGSLVQCFEWERVGEDFV 486
Query: 511 DMSEGGGLTLPKALPLRTLCRPRSNATHLL 536
DM+E G TLPKA+PLR +C+ RS L+
Sbjct: 487 DMTEDKGATLPKAIPLRAMCKARSIVDKLI 492
BLAST of Cmc07g0183581 vs. TAIR 10
Match:
AT4G37370.1 (cytochrome P450, family 81, subfamily D, polypeptide 8 )
HSP 1 Score: 443.7 bits (1140), Expect = 2.1e-124
Identity = 229/513 (44.64%), Postives = 338/513 (65.89%), Query Frame = 0
Query: 30 SMVISFLYLLILSILQIFTNHFLHRIRNLPPSPFLSLPIIGQLYLLKAPLHRTLSEISR- 89
+++ S L+ ++LS++ + L R NLPPSP SLP+IG L LLK P+HRT +S+
Sbjct: 5 TLIFSILF-VVLSLIYLIGK--LKRKPNLPPSPAWSLPVIGHLRLLKPPIHRTFLSLSQS 64
Query: 90 -RHGPILFFQFGFRPVLVVSSPSAAEDCLSKNDVIFANRPRLVSGKYLGYNYTSLLWAPY 149
+ PI + G R V V SS S AE+C +KNDV+ ANRP + K++ Y+YT+++ A Y
Sbjct: 65 LNNAPIFSLRLGNRLVFVNSSHSIAEECFTKNDVVLANRPNFILAKHVAYDYTTMIAASY 124
Query: 150 GEHWRNLRRISSLEILSSHRLQTLSSIRVDEVRKLIRRL---YNAENDVVDMRTEFFQLM 209
G+HWRNLRRI S+EI S+HRL + SIR DE+R+L+ RL ++ E VDM++ L
Sbjct: 125 GDHWRNLRRIGSVEIFSNHRLNSFLSIRKDEIRRLVFRLSRNFSQEFVKVDMKSMLSDLT 184
Query: 210 FNIMMRMIAGKRYYGEAVVADSEEGRRFQEIQEETFRLSGKTNLGDFLRVVKWVGLSKGI 269
FN ++RM+AGKRYYG+ V D E +R +++ + +G N D+L V++ L
Sbjct: 185 FNNILRMVAGKRYYGDG-VEDDPEAKRVRQLIADVVACAGAGNAVDYLPVLR---LVSDY 244
Query: 270 ENRLRELQIRRDDWMQSLIEEHRKKMDNAHSSSSSSIQGDAKKTMIEVLLSLQQMEPQYY 329
E R+++L R D+++Q L++E R+ + + TMI+ LL+LQ+ +P Y+
Sbjct: 245 ETRVKKLAGRLDEFLQGLVDEKREAKEKGN-------------TMIDHLLTLQESQPDYF 304
Query: 330 KDEYIRGLMLVLLLAGTEGSINTMEWLLSLLLNHPHSLRRAQMEIDNVVGKTNRLLEESD 389
D I+G ML L+LAGT+ S T+EW LS +LNHP L +A+ EID +G +RL++ESD
Sbjct: 305 TDRIIKGNMLALILAGTDTSAVTLEWALSNVLNHPDVLNKARDEIDRKIG-LDRLMDESD 364
Query: 390 LTHLPYLRSLIHETLRMYPPGPLLIPHESSQDCHVGGFHVPAGTMLFVNVWAIQNDPTVW 449
+++LPYL++++ ETLR+YP P+L+PH +S+DC V G+ +P GT+L NVWAI DP +W
Sbjct: 365 ISNLPYLQNIVSETLRLYPAAPMLLPHVASEDCKVAGYDMPRGTILLTNVWAIHRDPQLW 424
Query: 450 VEPEKFNPDRFKSDGEGFKWMPFGAGRRRCPGESLGLRVVGLVIGSLIQCFEWESMGGKC 509
+P F P+RF+ +GE K MPFG GRR CPG L R++ L +GSLIQC EWE +G +
Sbjct: 425 DDPMSFKPERFEKEGEAQKLMPFGLGRRACPGSGLAHRLINLTLGSLIQCLEWEKIGEE- 484
Query: 510 IDMSEGGGLTLPKALPLRTLCRPRSNATHLLSQ 538
+DMSEG G+T+PKA PL +CR R + + ++
Sbjct: 485 VDMSEGKGVTMPKAKPLEAMCRARPSVVKIFNE 495
BLAST of Cmc07g0183581 vs. TAIR 10
Match:
AT4G37360.1 (cytochrome P450, family 81, subfamily D, polypeptide 2 )
HSP 1 Score: 439.5 bits (1129), Expect = 3.9e-123
Identity = 234/505 (46.34%), Postives = 327/505 (64.75%), Query Frame = 0
Query: 31 MVISFLYLLILSILQIFTNHFLHRIRNLPPSPFLSLPIIGQLYLLKAPLHRTLSEISRRH 90
M+I ++LS+ IF + R NLPPSP +LP+IG L LLK PLHR +S+
Sbjct: 5 MLIFTFCFIVLSL--IFLIGRIKRKLNLPPSPAWALPVIGHLRLLKPPLHRVFLSVSQSL 64
Query: 91 G--PILFFQFGFRPVLVVSSPSAAEDCLSKNDVIFANRPRLVSGKYLGYNYTSLLWAPYG 150
G PI+ + G R + VVSS S AE+C +KNDVI ANR +S K++ Y ++++ A Y
Sbjct: 65 GDAPIISLRLGNRLLFVVSSHSIAEECFTKNDVILANRQTTISTKHISYGNSTVVSASYS 124
Query: 151 EHWRNLRRISSLEILSSHRLQTLSSIRVDEVRKLIRRLYNAEN---DVVDMRTEFFQLMF 210
EHWRNLRRI +LEI S+HRL + SSIR DE+R+LI RL + V+M++ F L F
Sbjct: 125 EHWRNLRRIGALEIFSAHRLNSFSSIRRDEIRRLIGRLLRNSSYGFTKVEMKSMFSDLTF 184
Query: 211 NIMMRMIAGKRYYGEAVVADSEEGRRFQEIQEETFRLSGKTNLGDFLRVVKWVGLSKGIE 270
N ++RM+AGK YYG+ D E +R + + E SG N D++ ++ W+ S E
Sbjct: 185 NNIIRMLAGKCYYGDG-KEDDPEAKRVRTLIAEAMSSSGPGNAADYIPILTWITYS---E 244
Query: 271 NRLRELQIRRDDWMQSLIEEHRKKMDNAHSSSSSSIQGDAKK--TMIEVLLSLQQMEPQY 330
R+++L R D+++Q L++E R +G KK TM++ LL LQ+ +P+Y
Sbjct: 245 TRIKKLAGRLDEFLQGLVDEKR--------------EGKEKKENTMVDHLLCLQETQPEY 304
Query: 331 YKDEYIRGLMLVLLLAGTEGSINTMEWLLSLLLNHPHSLRRAQMEIDNVVGKTNRLLEES 390
Y D I+G ML L+ GT+ + T+EW LS LLN+P L +A+ EID ++G +RLLEES
Sbjct: 305 YMDRIIKGTMLSLIAGGTDTTAVTLEWALSSLLNNPEVLNKARDEIDRMIG-VDRLLEES 364
Query: 391 DLTHLPYLRSLIHETLRMYPPGPLLIPHESSQDCHVGGFHVPAGTMLFVNVWAIQNDPTV 450
D+ +LPYL++++ ETLR+YP P+L+PH +S+DC VGG+ +P GTML N WAI DP +
Sbjct: 365 DIPNLPYLQNIVSETLRLYPAAPMLLPHVASKDCKVGGYDMPRGTMLLTNAWAIHRDPLL 424
Query: 451 WVEPEKFNPDRFKSDGEGFKWMPFGAGRRRCPGESLGLRVVGLVIGSLIQCFEWESMGGK 510
W +P F P+RF+ +GE K MPFG GRR CPG L R+V L +GSLIQCFEWE +G +
Sbjct: 425 WDDPTSFKPERFEKEGEAKKLMPFGLGRRACPGSGLAQRLVTLSLGSLIQCFEWERIGEE 484
Query: 511 CIDMSEGGGLTLPKALPLRTLCRPR 529
+DM+EG GLT+PKA PL +CR R
Sbjct: 485 EVDMTEGPGLTMPKARPLEAMCRAR 488
The following BLAST results are available for this feature:
Match Name | E-value | Identity | Description | |
XP_008452618.1 | 0.0e+00 | 99.26 | PREDICTED: cytochrome P450 81E8-like [Cucumis melo] | [more] |
XP_004141396.1 | 1.5e-273 | 93.11 | cytochrome P450 81Q32 [Cucumis sativus] >XP_011654142.1 cytochrome P450 81Q32 [C... | [more] |
XP_038899302.1 | 6.6e-258 | 86.33 | cytochrome P450 81Q32-like [Benincasa hispida] | [more] |
XP_023535659.1 | 1.6e-243 | 82.75 | isoflavone 2'-hydroxylase-like [Cucurbita pepo subsp. pepo] | [more] |
KAG7024285.1 | 2.7e-243 | 82.94 | Isoflavone 2'-hydroxylase, partial [Cucurbita argyrosperma subsp. argyrosperma] | [more] |
Match Name | E-value | Identity | Description | |
W8JMU7 | 1.2e-132 | 47.67 | Cytochrome P450 81Q32 OS=Catharanthus roseus OX=4058 GN=CYP81Q32 PE=2 SV=1 | [more] |
Q6WNQ8 | 2.6e-124 | 45.83 | Cytochrome P450 81E8 OS=Medicago truncatula OX=3880 GN=CYP81E8 PE=2 SV=1 | [more] |
Q6WNQ9 | 4.9e-123 | 45.94 | Isoflavone 3'-hydroxylase (Fragment) OS=Medicago truncatula OX=3880 GN=CYP81E9 P... | [more] |
P93147 | 2.1e-121 | 44.29 | Isoflavone 2'-hydroxylase OS=Glycyrrhiza echinata OX=46348 GN=CYP81E1 PE=1 SV=2 | [more] |
Q9FG65 | 1.4e-117 | 44.73 | Cytochrome P450 81D1 OS=Arabidopsis thaliana OX=3702 GN=CYP81D1 PE=2 SV=1 | [more] |
Match Name | E-value | Identity | Description | |
A0A1S3BVF3 | 0.0e+00 | 99.26 | cytochrome P450 81E8-like OS=Cucumis melo OX=3656 GN=LOC103493585 PE=3 SV=1 | [more] |
A0A0A0L0F1 | 7.1e-274 | 93.11 | Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G642290 PE=3 SV=1 | [more] |
A0A6J1FED9 | 5.0e-243 | 82.75 | isoflavone 2'-hydroxylase-like OS=Cucurbita moschata OX=3662 GN=LOC111443311 PE=... | [more] |
A0A6J1INS3 | 3.2e-242 | 82.55 | isoflavone 2'-hydroxylase-like OS=Cucurbita maxima OX=3661 GN=LOC111477207 PE=3 ... | [more] |
A0A6P4BCT8 | 3.4e-183 | 63.42 | cytochrome P450 81E8-like OS=Ziziphus jujuba OX=326968 GN=LOC107433678 PE=3 SV=1 | [more] |
Match Name | E-value | Identity | Description | |
AT4G37340.1 | 1.1e-125 | 45.33 | cytochrome P450, family 81, subfamily D, polypeptide 3 | [more] |
AT4G37320.1 | 1.4e-125 | 46.23 | cytochrome P450, family 81, subfamily D, polypeptide 5 | [more] |
AT4G37330.1 | 2.1e-124 | 45.49 | cytochrome P450, family 81, subfamily D, polypeptide 4 | [more] |
AT4G37370.1 | 2.1e-124 | 44.64 | cytochrome P450, family 81, subfamily D, polypeptide 8 | [more] |
AT4G37360.1 | 3.9e-123 | 46.34 | cytochrome P450, family 81, subfamily D, polypeptide 2 | [more] |