Cmc07g0182321 (gene) Melon (Charmono) v1.1

Overview
NameCmc07g0182321
Typegene
OrganismCucumis melo L. var. cantalupensis cv. Charmono (Melon (Charmono) v1.1)
DescriptionSAM_MT_RSMB_NOP domain-containing protein
LocationCMiso1.1chr07: 1325782 .. 1330532 (-)
RNA-Seq ExpressionCmc07g0182321
SyntenyCmc07g0182321
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CTTTGTACGATTAATTTGAAAAACCAAAGAGTTCTAACGGGGTTTAGCGCATGTCATATGATACGATGTCGTTTCGCAATACGACTTGGGTCAATGACCATTCATAAATATCGACTATCGGCCGGAGGTAGTTCGGACGAGGTGGCTGCTAAGAAGCCATGGGGCGCGTTAACAACAGAACCAAGGTATCGCCGTCGAGAGCCGCCTACAAATCAGAAAATCGGAAGATGAGCAACGCCGACCGTTCGGCATACTTCGCTAGGAGAGAGGCGGCGAAGATATTGCGGCGTGTTCTCGAGGGAGACGCTCAACGTCGAGCCATAGCCTCCATCAAGTCACTCGTTTATGCTCCATCTGTTCGGAATAAGAAGGGCACATTCGCTCTTGTTTGCAAGACTCTCAAATGTAAGTTCTGAACAAAGATTTTTTTTTTTCGCGGGAAGTGTCTTTCAAACGGATTCTCGTGTGGATGTCAATGAGACTATTGGTTTAAGTTGAACATCTTGCTTGGCGTTTGGATATTAGATGTTTAATATTGTTATGCGGCAGTTTTTATCTCTGCAACGAGCTAAATGGATAACATAACAATGAATTATGTGGAAATTTGGAATTCCATGTATTTTGATATGAATGATTCTTTGATAAGCTAGGTTATTACTAGTTAGCTTCCCTCGGTCCTCAGGTGATGTTTGCAGTGATTTAGCATAATTATTAAGGTTCATTTTGTAGCTTTCTGAGTTCTCTTGCTTTTGTTGCGTTGTTAGACCTCCCTGTGATCAAGGACGTACTTGAGGCTGCTGATTTATTGAGCAACAAATGGAAGGTACTCTTGCATTTTCTTTATAGCTGCTCTTGGATTTGGTCAGATGACAGTTATTCTCTTACCATGATCCTCTGTTATTCATGTTTCTCGAGATCCTCTGTAATTCATTTGTTTCTCAAGTTCGGGCTAATTCTTTCCGGATAGTTCTCTCACTTACTCCAAACATGCGTAACGCTATCCTGATATTTTTGCAGAGGCAGAAGGAATTAATTTATGTAATCATGTATGATATCCTCCTAGGCCAGGTATGTTATTCACACATTCATCTTACCTCAAGACGACGTTGGTTTATCCTTTTAAAAGATCGACTCTGAAGATGCTTTATGATAGTTAAACAAGGGAGAGGAGTATTTAATAAATTACATCGAATATTTGGACATTCTAAAAGTTATTTACTTGAAATTAGTTTTGTCTACTTTCAATAATCTACATATGGGTGTAATTTGCCAGCATGAAGATAGCTTAACTAACACAAGACACACTCTTGATCAAGAGGTCAAATGTTTAAATCTCCACCCCAAACTGTTGTACTGAAAATAGATGAGCGTGGTTTTGAGTTTGAACTAATTTTTTATCATCAAGAGTTACTGCCTACTGGATCCAGTAGGAGTTTGGAGGCCCGATCATGTAGTTCATAAATGAAGGATTTTTTTCCTTTGGTGAGACGTTTAGACTTTAGAATAAGCTTGCAGTAGAAAATACTTAGATAAGTTATCGTTACAAGAAAGTGTTAATGAATATGCAGAAGCTTCTGCTAGTCTATTGAGATTGCTGTAGCTTGCTATTTTACATTTGGTGATGATTGGTATTATTTTATGGAAACTGTGCGTAGAAAGCTCATCTAGCTGGCGATGCAGAGAAGTTTCTCATGCGGCAGCAAAGTGCTTTACAATCTGCTGTTACACAGCTTTTGTCGAAGAAAAAAGCAAACAGTATTGAAGATTTAATAACTCGTGGTGATGATGATCCTGGTGAGAGTTATGTATAAGTCTGTTCTATGCGGGTTTCCTCTAACTTAGTTATTAAGCCTACTCAGTTATGAAGACATAATCATCAATTCAGGTGTTTCAAGGCCCCGTTTTATTCGCGTGAATACTTTGAAGATGGATGTTCACACAGCAATAAAAGAATTAGGCAAAAGATATCCAGTGAGTTCCAACATCTTTCTCAATTATTATTGATGTGTATGCCAGTAGAGTAGAATTCAATAGCCACAATGGTTTTCGAAACCAGGACTAGGGATTTCACAGCTCAATACGCCCACGTTGCAAATGACTAAATTTCAATAGCCACAATGGGATTATCTTCACGAATTGCACACTTCATCATTTTGTACATATAAATGATCAACTGTTCTAAGTACCTCCTAGCCCCGTTAGTCCTTCTAAAAAATTCATAATATGGATGTAGGGATAGAATTTGTTATGTGTTTTGTTATTGGTAAGGATCGATTCCATTATGACACTCGCGAAGAATTCCTAAATGGTGCCGGTTATCATTTCAAACAGGTTCAGAAGGATGATATGGTTCCTGACCTCTTGATACTTCCCCCAGGCAGTGACTTACATGATCATCCTCTGGTTGCAGATGGAAGTATCTTCTTACAAGTAAGTATCTAGTTTTGTTCAGTCCATTGCCAAGAAGCATATGTTTAAGGCCAAAGGTAGCTGTTTTTTCTTTTTATTGTATAACAATAGAGTACTAATGTTCGTAGGGGAAGGCTAGTTCCATGGTTGCAGTAGCACTTGATCCCAAGCCAGGATGGGAAGTAAGATCATATCTACCTGTATTTACTGTAATTTCTCTGGTGTTAATAGTTTCCATTTTAATGGTTTGTATTATTGAAGGTCCTTGATGCATGTTCAGCACCGGGTAACAAAACTGTCCACCTTGCCTCTCTCATGAATGGAAAAGGAAGGGTCATTGCATGTGAACTGAATGAAAGCAGGGTCAAACGCTTACAACATACTATCAAACTATCTGGCGCTTCTAGTATCCTTCTTGGATAGTTTTGTCTGCCCGTATTGTTGATACCATACAACAACTCTCTATCTATCTGGAATCTTATGTGATTATTTTCTCTATTTGGATTAGTCGCCTTGCACGTGAGGTTGCCATTTTAATGAATTGAAAACTGACAGTAAGGCGATGATCTCATTTATGCCATTACCAGCTATTGTCCTAACTTACATGTTACTTTGATTAACCTTAATGGTGCAAGTTATAGACGTTTTGCATGGGGATTTCCTCAAGCTTAGTCCAAAAGATCCTTCGTTATCCAAAGTAAGTTTTGTTCAGCTCCACATTGATCATCTTTTCTTCATAAATAACTAAAGCTATTGCATGCATGCAACTATAATCATAGTTACCCCCTTCCTTAAAAAAAAAATCCTGATCTCTTAGATGGATTGGATCGGCCAATCCCATTGCCCCAGAACGATGGAAGTAATAACCATAGCTATTATTGCTTAGAGATTAGAATGCTTGGTGATTTCTAATTTGCTTGGGTCGGTAGACAAAAAGCCTAATCTTTTGCCACAGCGTACTGATGTTCAGCCGGAAAATGTTATGTTCTTGATGTCATCCACCATTGATATTTTAAGGTCCGTGCCATTCTTTTGGATCCTTCCTGCTCTGGATCTGGAACTGCGGCTGTCAGATTAGATCATTTGCTCCCGTCACATGTTGAAGGTAATTCTTAGCCTTGTCTTGATTGCAACATCCATAATCCATTATCTGCTCTATCTTCTTCTGGTTATTGATTGCTACCTCTACTATATCGTCATGAATTTTGCAGGGACAACCTCTGGAGATGACCTTGAACGACTAAACAAACTCGCTGCCTTTCAGAGAAAGGCTCTTGCACATGCATTTTCCTGTGAGTTACATTCCGTCGTTTATTTCTTTTCTTTCTCCTTTCTCCTTTTTTTCTTTATATATATATATATATATATATATATATATATATATATATGTATGTATGTATGTATGTGTATATATATATATATGTTTATATAATTATTTGGGGAATAGAGCTAAGAACAAAATAGATATAATATACTGAAGAACATTAGAAAATATCCACATGTTCTTGATGTTCGAAGGGTTTAAGGACTACCCTGTCCTTTCCTTCCTCGCCTCTCAGTCTCAACTTCCACTTTGTACTTGTACAATATATTGTGATATTTTACTCTACCCGTTCAAATAAACATTACATTGCCTGATCCTTGAACGTCCCTTGATAATTGAAATCCACACCTCCGATATCATCTCATTGCAGTTCCAGCCGTAGAGAGAATTGTTTACAGCACTTGTTCCATTCATCAAATTGAAAATGAAGATGTCGTCCAGTCACTATTACCTCTTGCTGAATCACATGGTTTTCAACTAGATACTCCGTTCCCACAGTGGCAGCGACGAGGTCTCCCAGTGTTCGCCGGCGGTAAGTGTCAACAAATTATTTGTGTTTTTCAAACTCACCCACTTCAATTTAGCTATAACTTCATTGCACAACTAAATATTCATGAATAATTTCTCATTATGCAGCTAACCACTTACTCCGAACCGATCCTGTTGAAGACAAAGAAGGGTTCTTCATAGCTCTATTTAAGAAGAAGCAGAACACGACGAACCCATTTCCCCGAAGTTTAAACATAGATAAAAGCTCAAGATATCGTAGTGGAGATGTCCGTAACAATCGGGCTTTAGCATGCCCTCCTACTAAGATCTCTAAACTGCGGTTGTATCAGTATGCACTTTCGACAAGGACACGATGGAGAAAGAACCACTCCATCCAAAACTAGATCGATAACTTGATTTTTTTTCAAAACTAAACATAGTAAAATCAATGGTTGGCTTCAGTTTCAAGTAAAAGGAGGACTTCATTAATCTGATAGCAATGTGTCATATCATAACCTTTCCAGTGCAT

mRNA sequence

CTTTGTACGATTAATTTGAAAAACCAAAGAGTTCTAACGGGGTTTAGCGCATGTCATATGATACGATGTCGTTTCGCAATACGACTTGGGTCAATGACCATTCATAAATATCGACTATCGGCCGGAGGTAGTTCGGACGAGGTGGCTGCTAAGAAGCCATGGGGCGCGTTAACAACAGAACCAAGGTATCGCCGTCGAGAGCCGCCTACAAATCAGAAAATCGGAAGATGAGCAACGCCGACCGTTCGGCATACTTCGCTAGGAGAGAGGCGGCGAAGATATTGCGGCGTGTTCTCGAGGGAGACGCTCAACGTCGAGCCATAGCCTCCATCAAGTCACTCGTTTATGCTCCATCTGTTCGGAATAAGAAGGGCACATTCGCTCTTGTTTGCAAGACTCTCAAATACCTCCCTGTGATCAAGGACGTACTTGAGGCTGCTGATTTATTGAGCAACAAATGGAAGAGGCAGAAGGAATTAATTTATGTAATCATGTATGATATCCTCCTAGGCCAGAAAGCTCATCTAGCTGGCGATGCAGAGAAGTTTCTCATGCGGCAGCAAAGTGCTTTACAATCTGCTGTTACACAGCTTTTGTCGAAGAAAAAAGCAAACAGTATTGAAGATTTAATAACTCGTGGTGATGATGATCCTGGTGTTTCAAGGCCCCGTTTTATTCGCGTGAATACTTTGAAGATGGATGTTCACACAGCAATAAAAGAATTAGGCAAAAGATATCCAGTTCAGAAGGATGATATGGTTCCTGACCTCTTGATACTTCCCCCAGGCAGTGACTTACATGATCATCCTCTGGTTGCAGATGGAAGTATCTTCTTACAAGGGAAGGCTAGTTCCATGGTTGCAGTAGCACTTGATCCCAAGCCAGGATGGGAAGTCCTTGATGCATGTTCAGCACCGGGTAACAAAACTGTCCACCTTGCCTCTCTCATGAATGGAAAAGGAAGGGTCATTGCATGTGAACTGAATGAAAGCAGGGTCAAACGCTTACAACATACTATCAAACTATCTGGCGCTTCTAACGTTTTGCATGGGGATTTCCTCAAGCTTAGTCCAAAAGATCCTTCGTTATCCAAAGTCCGTGCCATTCTTTTGGATCCTTCCTGCTCTGGATCTGGAACTGCGGCTGTCAGATTAGATCATTTGCTCCCGTCACATGTTGAAGGGACAACCTCTGGAGATGACCTTGAACGACTAAACAAACTCGCTGCCTTTCAGAGAAAGGCTCTTGCACATGCATTTTCCTTTCCAGCCGTAGAGAGAATTGTTTACAGCACTTGTTCCATTCATCAAATTGAAAATGAAGATGTCGTCCAGTCACTATTACCTCTTGCTGAATCACATGGTTTTCAACTAGATACTCCGTTCCCACAGTGGCAGCGACGAGGTCTCCCAGTGTTCGCCGGCGCTAACCACTTACTCCGAACCGATCCTGTTGAAGACAAAGAAGGGTTCTTCATAGCTCTATTTAAGAAGAAGCAGAACACGACGAACCCATTTCCCCGAAGTTTAAACATAGATAAAAGCTCAAGATATCGTAGTGGAGATGTCCGTAACAATCGGGCTTTAGCATGCCCTCCTACTAAGATCTCTAAACTGCGGTTGTATCAGTATGCACTTTCGACAAGGACACGATGGAGAAAGAACCACTCCATCCAAAACTAGATCGATAACTTGATTTTTTTTCAAAACTAAACATAGTAAAATCAATGGTTGGCTTCAGTTTCAAGTAAAAGGAGGACTTCATTAATCTGATAGCAATGTGTCATATCATAACCTTTCCAGTGCAT

Coding sequence (CDS)

ATGGGGCGCGTTAACAACAGAACCAAGGTATCGCCGTCGAGAGCCGCCTACAAATCAGAAAATCGGAAGATGAGCAACGCCGACCGTTCGGCATACTTCGCTAGGAGAGAGGCGGCGAAGATATTGCGGCGTGTTCTCGAGGGAGACGCTCAACGTCGAGCCATAGCCTCCATCAAGTCACTCGTTTATGCTCCATCTGTTCGGAATAAGAAGGGCACATTCGCTCTTGTTTGCAAGACTCTCAAATACCTCCCTGTGATCAAGGACGTACTTGAGGCTGCTGATTTATTGAGCAACAAATGGAAGAGGCAGAAGGAATTAATTTATGTAATCATGTATGATATCCTCCTAGGCCAGAAAGCTCATCTAGCTGGCGATGCAGAGAAGTTTCTCATGCGGCAGCAAAGTGCTTTACAATCTGCTGTTACACAGCTTTTGTCGAAGAAAAAAGCAAACAGTATTGAAGATTTAATAACTCGTGGTGATGATGATCCTGGTGTTTCAAGGCCCCGTTTTATTCGCGTGAATACTTTGAAGATGGATGTTCACACAGCAATAAAAGAATTAGGCAAAAGATATCCAGTTCAGAAGGATGATATGGTTCCTGACCTCTTGATACTTCCCCCAGGCAGTGACTTACATGATCATCCTCTGGTTGCAGATGGAAGTATCTTCTTACAAGGGAAGGCTAGTTCCATGGTTGCAGTAGCACTTGATCCCAAGCCAGGATGGGAAGTCCTTGATGCATGTTCAGCACCGGGTAACAAAACTGTCCACCTTGCCTCTCTCATGAATGGAAAAGGAAGGGTCATTGCATGTGAACTGAATGAAAGCAGGGTCAAACGCTTACAACATACTATCAAACTATCTGGCGCTTCTAACGTTTTGCATGGGGATTTCCTCAAGCTTAGTCCAAAAGATCCTTCGTTATCCAAAGTCCGTGCCATTCTTTTGGATCCTTCCTGCTCTGGATCTGGAACTGCGGCTGTCAGATTAGATCATTTGCTCCCGTCACATGTTGAAGGGACAACCTCTGGAGATGACCTTGAACGACTAAACAAACTCGCTGCCTTTCAGAGAAAGGCTCTTGCACATGCATTTTCCTTTCCAGCCGTAGAGAGAATTGTTTACAGCACTTGTTCCATTCATCAAATTGAAAATGAAGATGTCGTCCAGTCACTATTACCTCTTGCTGAATCACATGGTTTTCAACTAGATACTCCGTTCCCACAGTGGCAGCGACGAGGTCTCCCAGTGTTCGCCGGCGCTAACCACTTACTCCGAACCGATCCTGTTGAAGACAAAGAAGGGTTCTTCATAGCTCTATTTAAGAAGAAGCAGAACACGACGAACCCATTTCCCCGAAGTTTAAACATAGATAAAAGCTCAAGATATCGTAGTGGAGATGTCCGTAACAATCGGGCTTTAGCATGCCCTCCTACTAAGATCTCTAAACTGCGGTTGTATCAGTATGCACTTTCGACAAGGACACGATGGAGAAAGAACCACTCCATCCAAAACTAG

Protein sequence

MGRVNNRTKVSPSRAAYKSENRKMSNADRSAYFARREAAKILRRVLEGDAQRRAIASIKSLVYAPSVRNKKGTFALVCKTLKYLPVIKDVLEAADLLSNKWKRQKELIYVIMYDILLGQKAHLAGDAEKFLMRQQSALQSAVTQLLSKKKANSIEDLITRGDDDPGVSRPRFIRVNTLKMDVHTAIKELGKRYPVQKDDMVPDLLILPPGSDLHDHPLVADGSIFLQGKASSMVAVALDPKPGWEVLDACSAPGNKTVHLASLMNGKGRVIACELNESRVKRLQHTIKLSGASNVLHGDFLKLSPKDPSLSKVRAILLDPSCSGSGTAAVRLDHLLPSHVEGTTSGDDLERLNKLAAFQRKALAHAFSFPAVERIVYSTCSIHQIENEDVVQSLLPLAESHGFQLDTPFPQWQRRGLPVFAGANHLLRTDPVEDKEGFFIALFKKKQNTTNPFPRSLNIDKSSRYRSGDVRNNRALACPPTKISKLRLYQYALSTRTRWRKNHSIQN
Homology
BLAST of Cmc07g0182321 vs. NCBI nr
Match: XP_008452765.1 (PREDICTED: probable 28S rRNA (cytosine-C(5))-methyltransferase isoform X2 [Cucumis melo])

HSP 1 Score: 991.5 bits (2562), Expect = 2.7e-285
Identity = 503/507 (99.21%), Postives = 505/507 (99.61%), Query Frame = 0

Query: 1   MGRVNNRTKVSPSRAAYKSENRKMSNADRSAYFARREAAKILRRVLEGDAQRRAIASIKS 60
           MGRVNNRTKVSPSRAAYKSENRKMSNADRSAYFARREAAKILRRVLEGDAQRRAIASIKS
Sbjct: 1   MGRVNNRTKVSPSRAAYKSENRKMSNADRSAYFARREAAKILRRVLEGDAQRRAIASIKS 60

Query: 61  LVYAPSVRNKKGTFALVCKTLKYLPVIKDVLEAADLLSNKWKRQKELIYVIMYDILLGQK 120
           LVYAPSVRNKKGTFALVCKTLKYLPVIKDVLEAADLLSNKWKRQKELIYVIMYDILLGQK
Sbjct: 61  LVYAPSVRNKKGTFALVCKTLKYLPVIKDVLEAADLLSNKWKRQKELIYVIMYDILLGQK 120

Query: 121 AHLAGDAEKFLMRQQSALQSAVTQLLSKKKANSIEDLITRGDDDPGVSRPRFIRVNTLKM 180
           AHLAGDAEKFLMRQQSALQSAVTQLLSKKKAN+IEDLITRGDDDPGVSRPRFIRVNTLKM
Sbjct: 121 AHLAGDAEKFLMRQQSALQSAVTQLLSKKKANNIEDLITRGDDDPGVSRPRFIRVNTLKM 180

Query: 181 DVHTAIKELGKRYPVQKDDMVPDLLILPPGSDLHDHPLVADGSIFLQGKASSMVAVALDP 240
           DVHTAI+ELGKRYPV KDDMVPDLLILPPGSDLHDHPLVADGSIFLQGKASSMVAVALDP
Sbjct: 181 DVHTAIEELGKRYPVLKDDMVPDLLILPPGSDLHDHPLVADGSIFLQGKASSMVAVALDP 240

Query: 241 KPGWEVLDACSAPGNKTVHLASLMNGKGRVIACELNESRVKRLQHTIKLSGASNVLHGDF 300
           KPGWEVLDACSAPGNKTVHLASLMNGKGRVIACELNESRVKRLQHTIKLSGASNVLHGDF
Sbjct: 241 KPGWEVLDACSAPGNKTVHLASLMNGKGRVIACELNESRVKRLQHTIKLSGASNVLHGDF 300

Query: 301 LKLSPKDPSLSKVRAILLDPSCSGSGTAAVRLDHLLPSHVEGTTSGDDLERLNKLAAFQR 360
           L LSPKDPSLSKVRAILLDPSCSGSGTAAVRLDHLLPSHVEGTTSGDDLERLNKLAAFQR
Sbjct: 301 LNLSPKDPSLSKVRAILLDPSCSGSGTAAVRLDHLLPSHVEGTTSGDDLERLNKLAAFQR 360

Query: 361 KALAHAFSFPAVERIVYSTCSIHQIENEDVVQSLLPLAESHGFQLDTPFPQWQRRGLPVF 420
           KALAHAFSFPAVERIVYSTCSIHQIENEDVVQSLLPLAESHGFQLDTPFPQWQRRGLPVF
Sbjct: 361 KALAHAFSFPAVERIVYSTCSIHQIENEDVVQSLLPLAESHGFQLDTPFPQWQRRGLPVF 420

Query: 421 AGANHLLRTDPVEDKEGFFIALFKKKQNTTNPFPRSLNIDKSSRYRSGDVRNNRALACPP 480
           AGANHLLRTDPVEDKEGFFIALFKKKQNTTNPFPRSLNIDKSSRYRSGDVRNNRALACPP
Sbjct: 421 AGANHLLRTDPVEDKEGFFIALFKKKQNTTNPFPRSLNIDKSSRYRSGDVRNNRALACPP 480

Query: 481 TKISKLRLYQYALSTRTRWRKNHSIQN 508
           TKISKLRLYQYALSTRTRWRKNHSIQN
Sbjct: 481 TKISKLRLYQYALSTRTRWRKNHSIQN 507

BLAST of Cmc07g0182321 vs. NCBI nr
Match: XP_008452764.1 (PREDICTED: probable 28S rRNA (cytosine-C(5))-methyltransferase isoform X1 [Cucumis melo] >KAA0064520.1 putative 28S rRNA (cytosine-C(5))-methyltransferase isoform X1 [Cucumis melo var. makuwa] >TYK20070.1 putative 28S rRNA (cytosine-C(5))-methyltransferase isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 984.6 bits (2544), Expect = 3.3e-283
Identity = 502/509 (98.62%), Postives = 505/509 (99.21%), Query Frame = 0

Query: 1   MGRVNNRTKVSPSRAAYKSENRKMSNADRSAYFARREAAKILRRVLEGDAQRRAIASIKS 60
           MGRVNNRTKVSPSRAAYKSENRKMSNADRSAYFARREAAKILRRVLEGDAQRRAIASIKS
Sbjct: 1   MGRVNNRTKVSPSRAAYKSENRKMSNADRSAYFARREAAKILRRVLEGDAQRRAIASIKS 60

Query: 61  LVYAPSVRNKKGTFALVCKTLKYLPVIKDVLEAADLLSNKWKRQKELIYVIMYDILLGQK 120
           LVYAPSVRNKKGTFALVCKTLKYLPVIKDVLEAADLLSNKWKRQKELIYVIMYDILLGQK
Sbjct: 61  LVYAPSVRNKKGTFALVCKTLKYLPVIKDVLEAADLLSNKWKRQKELIYVIMYDILLGQK 120

Query: 121 AHLAGDAEKFLMRQQSALQSAVTQLLSKKKANSIEDLITRGDDDPGVSRPRFIRVNTLKM 180
           AHLAGDAEKFLMRQQSALQSAVTQLLSKKKAN+IEDLITRGDDDPGVSRPRFIRVNTLKM
Sbjct: 121 AHLAGDAEKFLMRQQSALQSAVTQLLSKKKANNIEDLITRGDDDPGVSRPRFIRVNTLKM 180

Query: 181 DVHTAIKELGKRYPVQKDDMVPDLLILPPGSDLHDHPLVADGSIFLQGKASSMVAVALDP 240
           DVHTAI+ELGKRYPV KDDMVPDLLILPPGSDLHDHPLVADGSIFLQGKASSMVAVALDP
Sbjct: 181 DVHTAIEELGKRYPVLKDDMVPDLLILPPGSDLHDHPLVADGSIFLQGKASSMVAVALDP 240

Query: 241 KPGWEVLDACSAPGNKTVHLASLMNGKGRVIACELNESRVKRLQHTIKLSGAS--NVLHG 300
           KPGWEVLDACSAPGNKTVHLASLMNGKGRVIACELNESRVKRLQHTIKLSGAS  +VLHG
Sbjct: 241 KPGWEVLDACSAPGNKTVHLASLMNGKGRVIACELNESRVKRLQHTIKLSGASIIDVLHG 300

Query: 301 DFLKLSPKDPSLSKVRAILLDPSCSGSGTAAVRLDHLLPSHVEGTTSGDDLERLNKLAAF 360
           DFL LSPKDPSLSKVRAILLDPSCSGSGTAAVRLDHLLPSHVEGTTSGDDLERLNKLAAF
Sbjct: 301 DFLNLSPKDPSLSKVRAILLDPSCSGSGTAAVRLDHLLPSHVEGTTSGDDLERLNKLAAF 360

Query: 361 QRKALAHAFSFPAVERIVYSTCSIHQIENEDVVQSLLPLAESHGFQLDTPFPQWQRRGLP 420
           QRKALAHAFSFPAVERIVYSTCSIHQIENEDVVQSLLPLAESHGFQLDTPFPQWQRRGLP
Sbjct: 361 QRKALAHAFSFPAVERIVYSTCSIHQIENEDVVQSLLPLAESHGFQLDTPFPQWQRRGLP 420

Query: 421 VFAGANHLLRTDPVEDKEGFFIALFKKKQNTTNPFPRSLNIDKSSRYRSGDVRNNRALAC 480
           VFAGANHLLRTDPVEDKEGFFIALFKKKQNTTNPFPRSLNIDKSSRYRSGDVRNNRALAC
Sbjct: 421 VFAGANHLLRTDPVEDKEGFFIALFKKKQNTTNPFPRSLNIDKSSRYRSGDVRNNRALAC 480

Query: 481 PPTKISKLRLYQYALSTRTRWRKNHSIQN 508
           PPTKISKLRLYQYALSTRTRWRKNHSIQN
Sbjct: 481 PPTKISKLRLYQYALSTRTRWRKNHSIQN 509

BLAST of Cmc07g0182321 vs. NCBI nr
Match: XP_004141333.2 (probable 28S rRNA (cytosine-C(5))-methyltransferase [Cucumis sativus])

HSP 1 Score: 921.0 bits (2379), Expect = 4.5e-264
Identity = 470/509 (92.34%), Postives = 486/509 (95.48%), Query Frame = 0

Query: 1   MGRVNNRTKVSPSRAAYKSENRKMSNADRSAYFARREAAKILRRVLEGDAQRRAIASIKS 60
           MGRVNNRTK S S AA KSE R+MSNADRSAYFARREAAK+LRRVLEGDAQRRAIASIK+
Sbjct: 34  MGRVNNRTKASASGAAGKSEMRRMSNADRSAYFARREAAKVLRRVLEGDAQRRAIASIKT 93

Query: 61  LVYAPSVRNKKGTFALVCKTLKYLPVIKDVLEAADLLSNKWKRQKELIYVIMYDILLGQK 120
           LVYAPSVRNKKGTFALVCKTLKYLPVIKDV+EAADLLSNKWKRQKELIYVIMYDILLGQK
Sbjct: 94  LVYAPSVRNKKGTFALVCKTLKYLPVIKDVVEAADLLSNKWKRQKELIYVIMYDILLGQK 153

Query: 121 AHLAGDAEKFLMRQQSALQSAVTQLLSKKKANSIEDLITRGDDDPGVSRPRFIRVNTLKM 180
            HLAGDAEKFLMRQQSALQSAVTQLLSKKKAN+IEDLI RGDD PGVSRPRF+RVNTLKM
Sbjct: 154 THLAGDAEKFLMRQQSALQSAVTQLLSKKKANNIEDLIARGDDGPGVSRPRFVRVNTLKM 213

Query: 181 DVHTAIKELGKRYPVQKDDMVPDLLILPPGSDLHDHPLVADGSIFLQGKASSMVAVALDP 240
           DVHTAI+ELGKRY VQKDDMV DLLILPPGSDLHDHPLVADGSIFLQGKASSMVAVALDP
Sbjct: 214 DVHTAIEELGKRYAVQKDDMVSDLLILPPGSDLHDHPLVADGSIFLQGKASSMVAVALDP 273

Query: 241 KPGWEVLDACSAPGNKTVHLASLMNGKGRVIACELNESRVKRLQHTIKLSGASN--VLHG 300
           KPGWEVLDACSAPGNKTVHLASLM+GKGRVIACELNE+RVKRL+HTIKLSGASN  VLHG
Sbjct: 274 KPGWEVLDACSAPGNKTVHLASLMHGKGRVIACELNENRVKRLRHTIKLSGASNIDVLHG 333

Query: 301 DFLKLSPKDPSLSKVRAILLDPSCSGSGTAAVRLDHLLPSHVEGTTSGDDLERLNKLAAF 360
           DFL L+PKDPSLSKVRAILLDPSCSGSGTAAVRLDHLLPSH EGT SGDDLERLNKLAAF
Sbjct: 334 DFLNLNPKDPSLSKVRAILLDPSCSGSGTAAVRLDHLLPSHAEGTISGDDLERLNKLAAF 393

Query: 361 QRKALAHAFSFPAVERIVYSTCSIHQIENEDVVQSLLPLAESHGFQLDTPFPQWQRRGLP 420
           QRKALAHAFSFPAVE+IVYSTCSIHQIENEDVVQS+LPLAES GFQLDTPFP+WQRRGLP
Sbjct: 394 QRKALAHAFSFPAVEKIVYSTCSIHQIENEDVVQSVLPLAESRGFQLDTPFPRWQRRGLP 453

Query: 421 VFAGANHLLRTDPVEDKEGFFIALFKKKQNTTNPFPRSLNIDKSSRYRSGDVRNNRALAC 480
           VFAGANHLLRTDPVEDKEGFFIALFKKKQNTTNPFPRS N DKSSRYR GDV N+ A+AC
Sbjct: 454 VFAGANHLLRTDPVEDKEGFFIALFKKKQNTTNPFPRSSNRDKSSRYRGGDVCNSWAVAC 513

Query: 481 PPTKISKLRLYQYALSTRTRWRKNHSIQN 508
           PPTKISKL LYQYALSTRTRWRKNH IQN
Sbjct: 514 PPTKISKLWLYQYALSTRTRWRKNHPIQN 542

BLAST of Cmc07g0182321 vs. NCBI nr
Match: KAE8649915.1 (hypothetical protein Csa_012918 [Cucumis sativus])

HSP 1 Score: 921.0 bits (2379), Expect = 4.5e-264
Identity = 470/509 (92.34%), Postives = 486/509 (95.48%), Query Frame = 0

Query: 1   MGRVNNRTKVSPSRAAYKSENRKMSNADRSAYFARREAAKILRRVLEGDAQRRAIASIKS 60
           MGRVNNRTK S S AA KSE R+MSNADRSAYFARREAAK+LRRVLEGDAQRRAIASIK+
Sbjct: 1   MGRVNNRTKASASGAAGKSEMRRMSNADRSAYFARREAAKVLRRVLEGDAQRRAIASIKT 60

Query: 61  LVYAPSVRNKKGTFALVCKTLKYLPVIKDVLEAADLLSNKWKRQKELIYVIMYDILLGQK 120
           LVYAPSVRNKKGTFALVCKTLKYLPVIKDV+EAADLLSNKWKRQKELIYVIMYDILLGQK
Sbjct: 61  LVYAPSVRNKKGTFALVCKTLKYLPVIKDVVEAADLLSNKWKRQKELIYVIMYDILLGQK 120

Query: 121 AHLAGDAEKFLMRQQSALQSAVTQLLSKKKANSIEDLITRGDDDPGVSRPRFIRVNTLKM 180
            HLAGDAEKFLMRQQSALQSAVTQLLSKKKAN+IEDLI RGDD PGVSRPRF+RVNTLKM
Sbjct: 121 THLAGDAEKFLMRQQSALQSAVTQLLSKKKANNIEDLIARGDDGPGVSRPRFVRVNTLKM 180

Query: 181 DVHTAIKELGKRYPVQKDDMVPDLLILPPGSDLHDHPLVADGSIFLQGKASSMVAVALDP 240
           DVHTAI+ELGKRY VQKDDMV DLLILPPGSDLHDHPLVADGSIFLQGKASSMVAVALDP
Sbjct: 181 DVHTAIEELGKRYAVQKDDMVSDLLILPPGSDLHDHPLVADGSIFLQGKASSMVAVALDP 240

Query: 241 KPGWEVLDACSAPGNKTVHLASLMNGKGRVIACELNESRVKRLQHTIKLSGASN--VLHG 300
           KPGWEVLDACSAPGNKTVHLASLM+GKGRVIACELNE+RVKRL+HTIKLSGASN  VLHG
Sbjct: 241 KPGWEVLDACSAPGNKTVHLASLMHGKGRVIACELNENRVKRLRHTIKLSGASNIDVLHG 300

Query: 301 DFLKLSPKDPSLSKVRAILLDPSCSGSGTAAVRLDHLLPSHVEGTTSGDDLERLNKLAAF 360
           DFL L+PKDPSLSKVRAILLDPSCSGSGTAAVRLDHLLPSH EGT SGDDLERLNKLAAF
Sbjct: 301 DFLNLNPKDPSLSKVRAILLDPSCSGSGTAAVRLDHLLPSHAEGTISGDDLERLNKLAAF 360

Query: 361 QRKALAHAFSFPAVERIVYSTCSIHQIENEDVVQSLLPLAESHGFQLDTPFPQWQRRGLP 420
           QRKALAHAFSFPAVE+IVYSTCSIHQIENEDVVQS+LPLAES GFQLDTPFP+WQRRGLP
Sbjct: 361 QRKALAHAFSFPAVEKIVYSTCSIHQIENEDVVQSVLPLAESRGFQLDTPFPRWQRRGLP 420

Query: 421 VFAGANHLLRTDPVEDKEGFFIALFKKKQNTTNPFPRSLNIDKSSRYRSGDVRNNRALAC 480
           VFAGANHLLRTDPVEDKEGFFIALFKKKQNTTNPFPRS N DKSSRYR GDV N+ A+AC
Sbjct: 421 VFAGANHLLRTDPVEDKEGFFIALFKKKQNTTNPFPRSSNRDKSSRYRGGDVCNSWAVAC 480

Query: 481 PPTKISKLRLYQYALSTRTRWRKNHSIQN 508
           PPTKISKL LYQYALSTRTRWRKNH IQN
Sbjct: 481 PPTKISKLWLYQYALSTRTRWRKNHPIQN 509

BLAST of Cmc07g0182321 vs. NCBI nr
Match: XP_038898177.1 (28S rRNA (cytosine-C(5))-methyltransferase-like isoform X1 [Benincasa hispida])

HSP 1 Score: 907.9 bits (2345), Expect = 3.9e-260
Identity = 462/507 (91.12%), Postives = 478/507 (94.28%), Query Frame = 0

Query: 1   MGRVNNRTKVSPSRAAYKSENRKMSNADRSAYFARREAAKILRRVLEGDAQRRAIASIKS 60
           MGRV NRTK S S  A KSENR+MSNADRSAYFARREAAKILRRVLEGDAQRRA+ASIKS
Sbjct: 1   MGRVTNRTKPSASGTAGKSENRRMSNADRSAYFARREAAKILRRVLEGDAQRRAVASIKS 60

Query: 61  LVYAPSVRNKKGTFALVCKTLKYLPVIKDVLEAADLLSNKWKRQKELIYVIMYDILLGQK 120
           LVYAPSVRNKKGTFALVCKTLKYLPVIKDVLEAADLLSNKWKRQKELIYVIMYDILLGQK
Sbjct: 61  LVYAPSVRNKKGTFALVCKTLKYLPVIKDVLEAADLLSNKWKRQKELIYVIMYDILLGQK 120

Query: 121 AHLAGDAEKFLMRQQSALQSAVTQLLSKKKANSIEDLITRGDDDPGVSRPRFIRVNTLKM 180
            HLAGDAEKFLMRQQSALQS V QLLSKKKA++IEDLI  G DDPGVSRPRFIRVNTLKM
Sbjct: 121 THLAGDAEKFLMRQQSALQSVVRQLLSKKKASNIEDLIAPG-DDPGVSRPRFIRVNTLKM 180

Query: 181 DVHTAIKELGKRYPVQKDDMVPDLLILPPGSDLHDHPLVADGSIFLQGKASSMVAVALDP 240
           +VHTAIKELGK+Y VQKDDMVPDLL+LPPGSDLHDHPLVADGSIF+QGKASSMVAVALDP
Sbjct: 181 EVHTAIKELGKKYAVQKDDMVPDLLVLPPGSDLHDHPLVADGSIFMQGKASSMVAVALDP 240

Query: 241 KPGWEVLDACSAPGNKTVHLASLMNGKGRVIACELNESRVKRLQHTIKLSGASNVLHGDF 300
           KPGWEVLDACSAPGNKTVHLASLM GKGR+IACELNE+RVKRLQHTIKLSGAS VLHGDF
Sbjct: 241 KPGWEVLDACSAPGNKTVHLASLMKGKGRIIACELNENRVKRLQHTIKLSGASKVLHGDF 300

Query: 301 LKLSPKDPSLSKVRAILLDPSCSGSGTAAVRLDHLLPSHVEGTTSGDDLERLNKLAAFQR 360
           L L+PKDPSLSKVRAILLDPSCSGSGTAA RLDHLLPSHVEG TS DDLERLNKLAAFQR
Sbjct: 301 LNLNPKDPSLSKVRAILLDPSCSGSGTAAARLDHLLPSHVEGKTSEDDLERLNKLAAFQR 360

Query: 361 KALAHAFSFPAVERIVYSTCSIHQIENEDVVQSLLPLAESHGFQLDTPFPQWQRRGLPVF 420
           KALAHAFSFPAVERIVYSTCSIHQ+ENEDVVQSLLPLAESHGFQLDTPFPQWQRRGLPVF
Sbjct: 361 KALAHAFSFPAVERIVYSTCSIHQVENEDVVQSLLPLAESHGFQLDTPFPQWQRRGLPVF 420

Query: 421 AGANHLLRTDPVEDKEGFFIALFKKKQNTTNPFPRSLNIDKSSRYRSGDVRNNRALACPP 480
           AGANHLLRTDPVEDKEGFFIALF KKQ+TT PFPR  N DKS RYR  +VRN+RA+ACPP
Sbjct: 421 AGANHLLRTDPVEDKEGFFIALFTKKQSTTQPFPRRSNRDKSFRYRGRNVRNSRAVACPP 480

Query: 481 TKISKLRLYQYALSTRTRWRKNHSIQN 508
           TKISKL LYQYA STRTRWRKNHSIQN
Sbjct: 481 TKISKLWLYQYAFSTRTRWRKNHSIQN 506

BLAST of Cmc07g0182321 vs. ExPASy Swiss-Prot
Match: Q8GYE8 (25S rRNA (cytosine-C(5))-methyltransferase NSUN5 OS=Arabidopsis thaliana OX=3702 GN=NSUN5 PE=1 SV=1)

HSP 1 Score: 596.3 bits (1536), Expect = 3.3e-169
Identity = 300/433 (69.28%), Postives = 354/433 (81.76%), Query Frame = 0

Query: 22  RKMSNADRSAYFARREAAKILRRVLEGDAQRRAIASIKSLVYAPSVRNKKGTFALVCKTL 81
           +K S+ +RSA +ARREAA ILR VL GDA+RRA+ASIKSLV +PSVRNK+GTFALVC+TL
Sbjct: 86  QKFSSLERSALYARREAANILRTVLRGDAERRAVASIKSLVLSPSVRNKRGTFALVCETL 145

Query: 82  KYLPVIKDVLEAADLLSNKWKRQKELIYVIMYDILLGQKAHLAGDAEKFLMRQQSALQSA 141
           KYL VIKDVL+ A++L++KWKRQ+ L++++ YDIL G+     GDAEKFLMR + AL S 
Sbjct: 146 KYLTVIKDVLDIANVLNSKWKRQEPLVFIVCYDILFGKDTPSIGDAEKFLMRHKEALLSG 205

Query: 142 VTQLLSKKKANSIEDLITRGDDDPGVSRPRFIRVNTLKMDVHTAIKELGKRYPVQKDDMV 201
           +  LL +KK +S++ L+  G    G  +PR++RVNTLKMDV +A++EL K Y VQKD+ V
Sbjct: 206 LATLLVRKKVDSVDQLL--GSKLTGHLKPRYVRVNTLKMDVDSAVQELEKHYTVQKDETV 265

Query: 202 PDLLILPPGSDLHDHPLVADGSIFLQGKASSMVAVALDPKPGWEVLDACSAPGNKTVHLA 261
           PDLL+LPPGSDLH H LVA+G IFLQGKASSMVA AL P+ GWEVLDACSAPGNKT+HLA
Sbjct: 266 PDLLVLPPGSDLHAHRLVANGRIFLQGKASSMVAAALQPQAGWEVLDACSAPGNKTIHLA 325

Query: 262 SLMNGKGRVIACELNESRVKRLQHTIKLSGASN--VLHGDFLKLSPKDPSLSKVRAILLD 321
           +LM G+G++IACELNE RVKRL+HTIKLSGASN  V HGDFL L+PKDPS +K+RAILLD
Sbjct: 326 ALMEGQGKIIACELNEERVKRLEHTIKLSGASNIEVCHGDFLGLNPKDPSFAKIRAILLD 385

Query: 322 PSCSGSGTAAVRLDHLLPSHVEGTTSGDDLERLNKLAAFQRKALAHAFSFPAVERIVYST 381
           PSCSGSGT   RLDHLLPSH E      D  RL+KLA FQ+KALAHA SFP VER+VYST
Sbjct: 386 PSCSGSGTITDRLDHLLPSHSEDNNMNYDSMRLHKLAVFQKKALAHALSFPKVERVVYST 445

Query: 382 CSIHQIENEDVVQSLLPLAESHGFQLDTPFPQWQRRGLPVFAGANHLLRTDPVEDKEGFF 441
           CSI+QIENEDVV S+LPLA S GF+L TPFPQWQRRGLPVFAG+ HLLR DPVEDKEGFF
Sbjct: 446 CSIYQIENEDVVSSVLPLASSLGFKLATPFPQWQRRGLPVFAGSEHLLRMDPVEDKEGFF 505

Query: 442 IALFKKKQNTTNP 453
           IALF +     NP
Sbjct: 506 IALFVRANKLDNP 516

BLAST of Cmc07g0182321 vs. ExPASy Swiss-Prot
Match: Q8K4F6 (28S rRNA (cytosine-C(5))-methyltransferase OS=Mus musculus OX=10090 GN=Nsun5 PE=1 SV=2)

HSP 1 Score: 266.5 bits (680), Expect = 6.0e-70
Identity = 172/441 (39.00%), Postives = 247/441 (56.01%), Query Frame = 0

Query: 45  VLEGDAQRRAIASIKSLVYAPSVRNKKGTFALVCKTLKYLPVIKDVLEAADLLSNKWKRQ 104
           VL G   R+   S+K LVY+ + +N K  +ALVC+T +Y  V+  V+ +A LL  + K +
Sbjct: 10  VLAGVESRQ--GSLKGLVYSSNFQNLKQLYALVCETQRYSAVLDAVIASAGLLRAEKKLR 69

Query: 105 KELIYVIMYDILLGQKAHLAGDAEKFLM-RQQSALQSAVTQLLSKKKANSIEDLITRGDD 164
             L  V++Y++LLG+     G   K L+ R Q+ L++ + +L   +  +  EDL+ +   
Sbjct: 70  PHLAKVLVYELLLGKGFRGGGGRWKALLGRHQARLKAELARLKVHRGVSRNEDLL-QESS 129

Query: 165 DPGVSR--PRFIRVNTLKMDVHTAI---KELGKRYPVQK----------------DDMVP 224
            PG +   PRF+RVNTLK     AI   K  G  Y  +                 D ++P
Sbjct: 130 RPGQAYQVPRFVRVNTLKTRPEDAIDYFKRQGFSYQGRASSLEDLRALKGQHFLLDPLLP 189

Query: 225 DLLILPPGSDLHDHPLVADGSIFLQGKASSMVAVALDPKPGWEVLDACSAPGNKTVHLAS 284
           +LL+ P  +DLH+HPL   G + LQ KAS + A+ L P PG  V+DAC+APGNKT ++A+
Sbjct: 190 ELLVFPAQTDLHEHPLYRAGHLILQDKASCLPAMLLSPPPGSHVIDACAAPGNKTSYIAA 249

Query: 285 LMNGKGRVIACELNESRVKRLQHTIKLSGAS--NVLHGDFLKLSPKDPSLSKVRAILLDP 344
           L+  +G++ A + +  R+  +   +  +G S   +   DFL +SP D   S+V+ ILLDP
Sbjct: 250 LLKNQGKIFAFDQDAKRLAAMATLVARAGVSCCELAEKDFLTVSPSDQRYSQVQYILLDP 309

Query: 345 SCSGSGTAAVRLDHLLPSHVEGTTSGDDLERLNKLAAFQRKALAHAFSFPAVERIVYSTC 404
           SCSGSG     L   L  H EGT S    ERL  LA FQ++AL HA  FP+++R+VYSTC
Sbjct: 310 SCSGSG----MLSRQLEEHGEGTPS---KERLQALAGFQQRALCHALRFPSLQRLVYSTC 369

Query: 405 SIHQIENEDVVQSLLPLAESHGFQLDTPFPQWQRRGLPVFAGANHLLRTDPVED-KEGFF 461
           S+ Q ENEDVVQ  L    S  F+L    P W  RGL  F G+ H LR  P      GFF
Sbjct: 370 SLCQEENEDVVQEALQ-HNSGTFRLAPVLPTWPHRGLSTFPGSEHCLRASPETTLTGGFF 429

BLAST of Cmc07g0182321 vs. ExPASy Swiss-Prot
Match: Q96P11 (28S rRNA (cytosine-C(5))-methyltransferase OS=Homo sapiens OX=9606 GN=NSUN5 PE=1 SV=2)

HSP 1 Score: 263.5 bits (672), Expect = 5.1e-69
Identity = 167/425 (39.29%), Postives = 239/425 (56.24%), Query Frame = 0

Query: 45  VLEGDAQRRAIASIKSLVYAPSVRNKKGTFALVCKTLKYLPVIKDVLEAADLLSNKWKRQ 104
           VL G   R+   SIK LVY+ + +N K  +ALVC+T +Y  V+  V+ +A LL  + K +
Sbjct: 10  VLAGVESRQ--GSIKGLVYSSNFQNVKQLYALVCETQRYSAVLDAVIASAGLLRAEKKLR 69

Query: 105 KELIYVIMYDILLGQKAHLAGDAEKFLM-RQQSALQSAVTQLLSKKKANSIEDLITRGD- 164
             L  V++Y++LLG+     G   K L+ R Q+ L++ + +L   +  +  EDL+  G  
Sbjct: 70  PHLAKVLVYELLLGKGFRGGGGRWKALLGRHQARLKAELARLKVHRGVSRNEDLLEVGSR 129

Query: 165 DDPGVSRPRFIRVNTLKM---DVHTAIKELGKRYPVQK----------------DDMVPD 224
             P    PRF+RVNTLK    DV    K  G  Y  +                 D ++P+
Sbjct: 130 PGPASQLPRFVRVNTLKTCSDDVVDYFKRQGFSYQGRASSLDDLRALKGKHFLLDPLMPE 189

Query: 225 LLILPPGSDLHDHPLVADGSIFLQGKASSMVAVALDPKPGWEVLDACSAPGNKTVHLASL 284
           LL+ P  +DLH+HPL   G + LQ +AS + A+ LDP PG  V+DAC+APGNKT HLA+L
Sbjct: 190 LLVFPAQTDLHEHPLYRAGHLILQDRASCLPAMLLDPPPGSHVIDACAAPGNKTSHLAAL 249

Query: 285 MNGKGRVIACELNESRVKRLQHTIKLSGAS--NVLHGDFLKLSPKDPSLSKVRAILLDPS 344
           +  +G++ A +L+  R+  +   +  +G S   +   DFL +SP DP   +V  ILLDPS
Sbjct: 250 LKNQGKIFAFDLDAKRLASMATLLARAGVSCCELAEEDFLAVSPSDPRYHEVHYILLDPS 309

Query: 345 CSGSGTAAVRLDHLLPSHVEGTTSGDDLERLNKLAAFQRKALAHAFSFPAVERIVYSTCS 404
           CSGSG  + +L+   P    GT S     RL+ LA FQ++AL HA +FP+++R+VYSTCS
Sbjct: 310 CSGSGMPSRQLEE--PG--AGTPSP---VRLHALAGFQQRALCHALTFPSLQRLVYSTCS 369

Query: 405 IHQIENEDVVQSLLPLAESHGFQLDTPFPQWQRRGLPVFAGANHLLRTDPVED-KEGFFI 446
           + Q ENEDVV+  L       F+L    P W  RGL  F GA H LR  P      GFF+
Sbjct: 370 LCQEENEDVVRDALQ-QNPGAFRLAPALPAWPHRGLSTFPGAEHCLRASPETTLSSGFFV 424

BLAST of Cmc07g0182321 vs. ExPASy Swiss-Prot
Match: O14039 (25S rRNA (cytosine-C(5))-methyltransferase rcm1 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=rcm1 PE=3 SV=3)

HSP 1 Score: 211.8 bits (538), Expect = 1.7e-53
Identity = 144/431 (33.41%), Postives = 223/431 (51.74%), Query Frame = 0

Query: 57  SIKSLVYAPSVRNKKGTFALVCKTLKYLPVIKDVLEAADLLSNKWKRQKELIYVIMYDIL 116
           SIK L +     + K T+ALVC+TLKY PV+ +++  ++LL  + K ++ L  V+++D+L
Sbjct: 20  SIKQLAFNSKKHDPKRTYALVCETLKYKPVLDEIIARSELLVLEKKLKENLARVLVHDLL 79

Query: 117 LGQK--AHLAGDAEKFLMRQQSALQSAVTQLLSKKKANSIEDLITRGDDDPGVSRPRFIR 176
           + ++  +   G  ++ ++R ++ L +   +L  KK   S E+L  +      VS PR++R
Sbjct: 80  MSKRGLSISNGPIKECILRHKTRLNAEFVKLKVKKGVKSHEELALKNP----VSLPRWLR 139

Query: 177 VNTLK-----------MDVHTAIKELGKRYPVQKDDMVPDLLILPPGSDLHDHPLVADGS 236
           +NT+K           +D  ++I+ELG       DD V +L+ + P   + ++ L  +G 
Sbjct: 140 INTIKSTKDEVLQGLGLDKVSSIEELGPD-KFYIDDCVENLIAIDPSFPIVENSLYKEGK 199

Query: 237 IFLQGKASSMVAVALDPKPG--WEVLDACSAPGNKTVHLASLMNGKGRVIACELNESRVK 296
           + +Q KAS   A  L    G   +++D C+APGNKT HLA+    K  + A E +  RV+
Sbjct: 200 VIIQDKASCFPAAVLAGLTGHVGDIIDGCAAPGNKTTHLAACF-PKSHIFAFERDAKRVQ 259

Query: 297 RLQHTIKLSGASNVL--HGDFLKLSPKDPSLSKVRAILLDPSCSGSGTAAVRLDHLLPSH 356
            L+  + +SGA+NV   H DF    PK      V  ILLDPSCSGSG  + R D+LL + 
Sbjct: 260 TLRKMVGISGANNVTIEHQDFTLTDPKSDLYRNVTHILLDPSCSGSGIVS-RQDYLLGNE 319

Query: 357 VEGTTSGDDLERLNKLAAFQRKALAHAFSFPAVERIVYSTCSIHQIENEDVVQSLLPLAE 416
            + T   +D ERL  L +FQ   L HA  FP    + YSTCS+H++ENE VV  +L    
Sbjct: 320 QDVT---EDTERLENLCSFQSTILKHALQFPNCRHVTYSTCSVHRLENEQVVCEVLSQEP 379

Query: 417 SHGF-QLDTPFPQWQRRGLPVFAG----ANHLLRTDP-VEDKEGFFIALFKKKQNTTNPF 465
                 L    P W+ RG+P +      A  ++R  P      GFF+A     Q     F
Sbjct: 380 DWKCNSLTKTLPNWKTRGIPEYCAQPSMAEGMIRCKPGAGGTIGFFVANLYHPQREQETF 439

BLAST of Cmc07g0182321 vs. ExPASy Swiss-Prot
Match: Q9NAA7 (26S rRNA (cytosine-C(5))-methyltransferase nsun-5 OS=Caenorhabditis elegans OX=6239 GN=nsun-5 PE=1 SV=2)

HSP 1 Score: 196.4 bits (498), Expect = 7.6e-49
Identity = 142/446 (31.84%), Postives = 217/446 (48.65%), Query Frame = 0

Query: 37  EAAKILRRVLEGDAQRRAIASIKSLVYAPSVRNKKGTFALVCKTLKYLPVIKDVLEAADL 96
           E A+I+R VL  +       S+++ VY  S +NKK    L C++LK+ PV  ++L+  +L
Sbjct: 9   EVAEIIRCVLAKE------KSVRNAVYGSSYKNKKALLRLSCESLKFRPVFDEILQDKEL 68

Query: 97  LSNKWKR----QKELIYVIMYDILLGQKAHLAGDAEKFLMRQQSALQSAVTQLLSKKKAN 156
            S K         EL+YV+MY+ L+G          K ++ ++      V   + + +  
Sbjct: 69  KSMKRDANIGGSVELLYVLMYETLVGSGLTRCSQELKSVISRRIQRIKEVEHAM-QDEGR 128

Query: 157 SIEDLITRGDDDPGVSRPRFIRVNTLKMDVHTAIKELG---------------------- 216
            I+ +    D    +  PR+ R+NTLK     A+K L                       
Sbjct: 129 GIKAMKEADDGMKKIQIPRYARINTLKWTADEAMKTLETEKWKILGTLKPENFAEMVTKM 188

Query: 217 KRYPVQKDDMVPDLLILPPG-SDLHDHPLVADGSIFLQGKASSMVAVALDPKPGWEVLDA 276
           K   V  D  V +L+I  P   + +++ +V    + LQ KAS + A  L+P+PG +V D 
Sbjct: 189 KDDEVYVDPHVENLIIFAPNIQNFYEYWMVEQRYLILQDKASCLPAFLLNPRPGSQVFDT 248

Query: 277 CSAPGNKTVHLASLMNGKGRVIACELNESRV---KRLQHTIKLSGASNVLHGDFLKLSPK 336
           C+APG KT H A++M  +G+V A +    RV   K+L    K++ AS+   GDFLK    
Sbjct: 249 CAAPGMKTSHAAAIMENQGKVWAMDRAADRVATMKQLLDASKVAIASSFC-GDFLKTDVT 308

Query: 337 DPSLSKVRAILLDPSCSGSGTAAVRLDHLLPSHVEGTTSGDDLERLNKLAAFQRKALAHA 396
           D   SKV+  ++DP CSGSG    R+D +       T    + ERL KL   Q   L HA
Sbjct: 309 DKKFSKVKFAIVDPPCSGSGIVK-RMDEI-------TGGNAEKERLEKLKNLQAMILKHA 368

Query: 397 FSFPAVERIVYSTCSIHQIENEDVVQSLL--PLAESHGFQLDTPFPQWQRRGLPVFAGAN 450
              P ++R VYSTCS+H+ ENE VV  +L       +        P+W  RGL  +    
Sbjct: 369 LKLPGLKRAVYSTCSVHEEENEQVVDEVLLDTYVRQNYVLKKNVLPEWTYRGLKTYEVGE 428

BLAST of Cmc07g0182321 vs. ExPASy TrEMBL
Match: A0A1S3BVF0 (probable 28S rRNA (Cytosine-C(5))-methyltransferase isoform X2 OS=Cucumis melo OX=3656 GN=LOC103493685 PE=3 SV=1)

HSP 1 Score: 991.5 bits (2562), Expect = 1.3e-285
Identity = 503/507 (99.21%), Postives = 505/507 (99.61%), Query Frame = 0

Query: 1   MGRVNNRTKVSPSRAAYKSENRKMSNADRSAYFARREAAKILRRVLEGDAQRRAIASIKS 60
           MGRVNNRTKVSPSRAAYKSENRKMSNADRSAYFARREAAKILRRVLEGDAQRRAIASIKS
Sbjct: 1   MGRVNNRTKVSPSRAAYKSENRKMSNADRSAYFARREAAKILRRVLEGDAQRRAIASIKS 60

Query: 61  LVYAPSVRNKKGTFALVCKTLKYLPVIKDVLEAADLLSNKWKRQKELIYVIMYDILLGQK 120
           LVYAPSVRNKKGTFALVCKTLKYLPVIKDVLEAADLLSNKWKRQKELIYVIMYDILLGQK
Sbjct: 61  LVYAPSVRNKKGTFALVCKTLKYLPVIKDVLEAADLLSNKWKRQKELIYVIMYDILLGQK 120

Query: 121 AHLAGDAEKFLMRQQSALQSAVTQLLSKKKANSIEDLITRGDDDPGVSRPRFIRVNTLKM 180
           AHLAGDAEKFLMRQQSALQSAVTQLLSKKKAN+IEDLITRGDDDPGVSRPRFIRVNTLKM
Sbjct: 121 AHLAGDAEKFLMRQQSALQSAVTQLLSKKKANNIEDLITRGDDDPGVSRPRFIRVNTLKM 180

Query: 181 DVHTAIKELGKRYPVQKDDMVPDLLILPPGSDLHDHPLVADGSIFLQGKASSMVAVALDP 240
           DVHTAI+ELGKRYPV KDDMVPDLLILPPGSDLHDHPLVADGSIFLQGKASSMVAVALDP
Sbjct: 181 DVHTAIEELGKRYPVLKDDMVPDLLILPPGSDLHDHPLVADGSIFLQGKASSMVAVALDP 240

Query: 241 KPGWEVLDACSAPGNKTVHLASLMNGKGRVIACELNESRVKRLQHTIKLSGASNVLHGDF 300
           KPGWEVLDACSAPGNKTVHLASLMNGKGRVIACELNESRVKRLQHTIKLSGASNVLHGDF
Sbjct: 241 KPGWEVLDACSAPGNKTVHLASLMNGKGRVIACELNESRVKRLQHTIKLSGASNVLHGDF 300

Query: 301 LKLSPKDPSLSKVRAILLDPSCSGSGTAAVRLDHLLPSHVEGTTSGDDLERLNKLAAFQR 360
           L LSPKDPSLSKVRAILLDPSCSGSGTAAVRLDHLLPSHVEGTTSGDDLERLNKLAAFQR
Sbjct: 301 LNLSPKDPSLSKVRAILLDPSCSGSGTAAVRLDHLLPSHVEGTTSGDDLERLNKLAAFQR 360

Query: 361 KALAHAFSFPAVERIVYSTCSIHQIENEDVVQSLLPLAESHGFQLDTPFPQWQRRGLPVF 420
           KALAHAFSFPAVERIVYSTCSIHQIENEDVVQSLLPLAESHGFQLDTPFPQWQRRGLPVF
Sbjct: 361 KALAHAFSFPAVERIVYSTCSIHQIENEDVVQSLLPLAESHGFQLDTPFPQWQRRGLPVF 420

Query: 421 AGANHLLRTDPVEDKEGFFIALFKKKQNTTNPFPRSLNIDKSSRYRSGDVRNNRALACPP 480
           AGANHLLRTDPVEDKEGFFIALFKKKQNTTNPFPRSLNIDKSSRYRSGDVRNNRALACPP
Sbjct: 421 AGANHLLRTDPVEDKEGFFIALFKKKQNTTNPFPRSLNIDKSSRYRSGDVRNNRALACPP 480

Query: 481 TKISKLRLYQYALSTRTRWRKNHSIQN 508
           TKISKLRLYQYALSTRTRWRKNHSIQN
Sbjct: 481 TKISKLRLYQYALSTRTRWRKNHSIQN 507

BLAST of Cmc07g0182321 vs. ExPASy TrEMBL
Match: A0A5D3D9A2 (Putative 28S rRNA (Cytosine-C(5))-methyltransferase isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold134G001930 PE=3 SV=1)

HSP 1 Score: 984.6 bits (2544), Expect = 1.6e-283
Identity = 502/509 (98.62%), Postives = 505/509 (99.21%), Query Frame = 0

Query: 1   MGRVNNRTKVSPSRAAYKSENRKMSNADRSAYFARREAAKILRRVLEGDAQRRAIASIKS 60
           MGRVNNRTKVSPSRAAYKSENRKMSNADRSAYFARREAAKILRRVLEGDAQRRAIASIKS
Sbjct: 1   MGRVNNRTKVSPSRAAYKSENRKMSNADRSAYFARREAAKILRRVLEGDAQRRAIASIKS 60

Query: 61  LVYAPSVRNKKGTFALVCKTLKYLPVIKDVLEAADLLSNKWKRQKELIYVIMYDILLGQK 120
           LVYAPSVRNKKGTFALVCKTLKYLPVIKDVLEAADLLSNKWKRQKELIYVIMYDILLGQK
Sbjct: 61  LVYAPSVRNKKGTFALVCKTLKYLPVIKDVLEAADLLSNKWKRQKELIYVIMYDILLGQK 120

Query: 121 AHLAGDAEKFLMRQQSALQSAVTQLLSKKKANSIEDLITRGDDDPGVSRPRFIRVNTLKM 180
           AHLAGDAEKFLMRQQSALQSAVTQLLSKKKAN+IEDLITRGDDDPGVSRPRFIRVNTLKM
Sbjct: 121 AHLAGDAEKFLMRQQSALQSAVTQLLSKKKANNIEDLITRGDDDPGVSRPRFIRVNTLKM 180

Query: 181 DVHTAIKELGKRYPVQKDDMVPDLLILPPGSDLHDHPLVADGSIFLQGKASSMVAVALDP 240
           DVHTAI+ELGKRYPV KDDMVPDLLILPPGSDLHDHPLVADGSIFLQGKASSMVAVALDP
Sbjct: 181 DVHTAIEELGKRYPVLKDDMVPDLLILPPGSDLHDHPLVADGSIFLQGKASSMVAVALDP 240

Query: 241 KPGWEVLDACSAPGNKTVHLASLMNGKGRVIACELNESRVKRLQHTIKLSGAS--NVLHG 300
           KPGWEVLDACSAPGNKTVHLASLMNGKGRVIACELNESRVKRLQHTIKLSGAS  +VLHG
Sbjct: 241 KPGWEVLDACSAPGNKTVHLASLMNGKGRVIACELNESRVKRLQHTIKLSGASIIDVLHG 300

Query: 301 DFLKLSPKDPSLSKVRAILLDPSCSGSGTAAVRLDHLLPSHVEGTTSGDDLERLNKLAAF 360
           DFL LSPKDPSLSKVRAILLDPSCSGSGTAAVRLDHLLPSHVEGTTSGDDLERLNKLAAF
Sbjct: 301 DFLNLSPKDPSLSKVRAILLDPSCSGSGTAAVRLDHLLPSHVEGTTSGDDLERLNKLAAF 360

Query: 361 QRKALAHAFSFPAVERIVYSTCSIHQIENEDVVQSLLPLAESHGFQLDTPFPQWQRRGLP 420
           QRKALAHAFSFPAVERIVYSTCSIHQIENEDVVQSLLPLAESHGFQLDTPFPQWQRRGLP
Sbjct: 361 QRKALAHAFSFPAVERIVYSTCSIHQIENEDVVQSLLPLAESHGFQLDTPFPQWQRRGLP 420

Query: 421 VFAGANHLLRTDPVEDKEGFFIALFKKKQNTTNPFPRSLNIDKSSRYRSGDVRNNRALAC 480
           VFAGANHLLRTDPVEDKEGFFIALFKKKQNTTNPFPRSLNIDKSSRYRSGDVRNNRALAC
Sbjct: 421 VFAGANHLLRTDPVEDKEGFFIALFKKKQNTTNPFPRSLNIDKSSRYRSGDVRNNRALAC 480

Query: 481 PPTKISKLRLYQYALSTRTRWRKNHSIQN 508
           PPTKISKLRLYQYALSTRTRWRKNHSIQN
Sbjct: 481 PPTKISKLRLYQYALSTRTRWRKNHSIQN 509

BLAST of Cmc07g0182321 vs. ExPASy TrEMBL
Match: A0A1S3BUJ8 (probable 28S rRNA (Cytosine-C(5))-methyltransferase isoform X1 OS=Cucumis melo OX=3656 GN=LOC103493685 PE=3 SV=1)

HSP 1 Score: 984.6 bits (2544), Expect = 1.6e-283
Identity = 502/509 (98.62%), Postives = 505/509 (99.21%), Query Frame = 0

Query: 1   MGRVNNRTKVSPSRAAYKSENRKMSNADRSAYFARREAAKILRRVLEGDAQRRAIASIKS 60
           MGRVNNRTKVSPSRAAYKSENRKMSNADRSAYFARREAAKILRRVLEGDAQRRAIASIKS
Sbjct: 1   MGRVNNRTKVSPSRAAYKSENRKMSNADRSAYFARREAAKILRRVLEGDAQRRAIASIKS 60

Query: 61  LVYAPSVRNKKGTFALVCKTLKYLPVIKDVLEAADLLSNKWKRQKELIYVIMYDILLGQK 120
           LVYAPSVRNKKGTFALVCKTLKYLPVIKDVLEAADLLSNKWKRQKELIYVIMYDILLGQK
Sbjct: 61  LVYAPSVRNKKGTFALVCKTLKYLPVIKDVLEAADLLSNKWKRQKELIYVIMYDILLGQK 120

Query: 121 AHLAGDAEKFLMRQQSALQSAVTQLLSKKKANSIEDLITRGDDDPGVSRPRFIRVNTLKM 180
           AHLAGDAEKFLMRQQSALQSAVTQLLSKKKAN+IEDLITRGDDDPGVSRPRFIRVNTLKM
Sbjct: 121 AHLAGDAEKFLMRQQSALQSAVTQLLSKKKANNIEDLITRGDDDPGVSRPRFIRVNTLKM 180

Query: 181 DVHTAIKELGKRYPVQKDDMVPDLLILPPGSDLHDHPLVADGSIFLQGKASSMVAVALDP 240
           DVHTAI+ELGKRYPV KDDMVPDLLILPPGSDLHDHPLVADGSIFLQGKASSMVAVALDP
Sbjct: 181 DVHTAIEELGKRYPVLKDDMVPDLLILPPGSDLHDHPLVADGSIFLQGKASSMVAVALDP 240

Query: 241 KPGWEVLDACSAPGNKTVHLASLMNGKGRVIACELNESRVKRLQHTIKLSGAS--NVLHG 300
           KPGWEVLDACSAPGNKTVHLASLMNGKGRVIACELNESRVKRLQHTIKLSGAS  +VLHG
Sbjct: 241 KPGWEVLDACSAPGNKTVHLASLMNGKGRVIACELNESRVKRLQHTIKLSGASIIDVLHG 300

Query: 301 DFLKLSPKDPSLSKVRAILLDPSCSGSGTAAVRLDHLLPSHVEGTTSGDDLERLNKLAAF 360
           DFL LSPKDPSLSKVRAILLDPSCSGSGTAAVRLDHLLPSHVEGTTSGDDLERLNKLAAF
Sbjct: 301 DFLNLSPKDPSLSKVRAILLDPSCSGSGTAAVRLDHLLPSHVEGTTSGDDLERLNKLAAF 360

Query: 361 QRKALAHAFSFPAVERIVYSTCSIHQIENEDVVQSLLPLAESHGFQLDTPFPQWQRRGLP 420
           QRKALAHAFSFPAVERIVYSTCSIHQIENEDVVQSLLPLAESHGFQLDTPFPQWQRRGLP
Sbjct: 361 QRKALAHAFSFPAVERIVYSTCSIHQIENEDVVQSLLPLAESHGFQLDTPFPQWQRRGLP 420

Query: 421 VFAGANHLLRTDPVEDKEGFFIALFKKKQNTTNPFPRSLNIDKSSRYRSGDVRNNRALAC 480
           VFAGANHLLRTDPVEDKEGFFIALFKKKQNTTNPFPRSLNIDKSSRYRSGDVRNNRALAC
Sbjct: 421 VFAGANHLLRTDPVEDKEGFFIALFKKKQNTTNPFPRSLNIDKSSRYRSGDVRNNRALAC 480

Query: 481 PPTKISKLRLYQYALSTRTRWRKNHSIQN 508
           PPTKISKLRLYQYALSTRTRWRKNHSIQN
Sbjct: 481 PPTKISKLRLYQYALSTRTRWRKNHSIQN 509

BLAST of Cmc07g0182321 vs. ExPASy TrEMBL
Match: A0A0A0L2U2 (SAM_MT_RSMB_NOP domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G646330 PE=3 SV=1)

HSP 1 Score: 921.0 bits (2379), Expect = 2.2e-264
Identity = 470/509 (92.34%), Postives = 486/509 (95.48%), Query Frame = 0

Query: 1   MGRVNNRTKVSPSRAAYKSENRKMSNADRSAYFARREAAKILRRVLEGDAQRRAIASIKS 60
           MGRVNNRTK S S AA KSE R+MSNADRSAYFARREAAK+LRRVLEGDAQRRAIASIK+
Sbjct: 26  MGRVNNRTKASASGAAGKSEMRRMSNADRSAYFARREAAKVLRRVLEGDAQRRAIASIKT 85

Query: 61  LVYAPSVRNKKGTFALVCKTLKYLPVIKDVLEAADLLSNKWKRQKELIYVIMYDILLGQK 120
           LVYAPSVRNKKGTFALVCKTLKYLPVIKDV+EAADLLSNKWKRQKELIYVIMYDILLGQK
Sbjct: 86  LVYAPSVRNKKGTFALVCKTLKYLPVIKDVVEAADLLSNKWKRQKELIYVIMYDILLGQK 145

Query: 121 AHLAGDAEKFLMRQQSALQSAVTQLLSKKKANSIEDLITRGDDDPGVSRPRFIRVNTLKM 180
            HLAGDAEKFLMRQQSALQSAVTQLLSKKKAN+IEDLI RGDD PGVSRPRF+RVNTLKM
Sbjct: 146 THLAGDAEKFLMRQQSALQSAVTQLLSKKKANNIEDLIARGDDGPGVSRPRFVRVNTLKM 205

Query: 181 DVHTAIKELGKRYPVQKDDMVPDLLILPPGSDLHDHPLVADGSIFLQGKASSMVAVALDP 240
           DVHTAI+ELGKRY VQKDDMV DLLILPPGSDLHDHPLVADGSIFLQGKASSMVAVALDP
Sbjct: 206 DVHTAIEELGKRYAVQKDDMVSDLLILPPGSDLHDHPLVADGSIFLQGKASSMVAVALDP 265

Query: 241 KPGWEVLDACSAPGNKTVHLASLMNGKGRVIACELNESRVKRLQHTIKLSGASN--VLHG 300
           KPGWEVLDACSAPGNKTVHLASLM+GKGRVIACELNE+RVKRL+HTIKLSGASN  VLHG
Sbjct: 266 KPGWEVLDACSAPGNKTVHLASLMHGKGRVIACELNENRVKRLRHTIKLSGASNIDVLHG 325

Query: 301 DFLKLSPKDPSLSKVRAILLDPSCSGSGTAAVRLDHLLPSHVEGTTSGDDLERLNKLAAF 360
           DFL L+PKDPSLSKVRAILLDPSCSGSGTAAVRLDHLLPSH EGT SGDDLERLNKLAAF
Sbjct: 326 DFLNLNPKDPSLSKVRAILLDPSCSGSGTAAVRLDHLLPSHAEGTISGDDLERLNKLAAF 385

Query: 361 QRKALAHAFSFPAVERIVYSTCSIHQIENEDVVQSLLPLAESHGFQLDTPFPQWQRRGLP 420
           QRKALAHAFSFPAVE+IVYSTCSIHQIENEDVVQS+LPLAES GFQLDTPFP+WQRRGLP
Sbjct: 386 QRKALAHAFSFPAVEKIVYSTCSIHQIENEDVVQSVLPLAESRGFQLDTPFPRWQRRGLP 445

Query: 421 VFAGANHLLRTDPVEDKEGFFIALFKKKQNTTNPFPRSLNIDKSSRYRSGDVRNNRALAC 480
           VFAGANHLLRTDPVEDKEGFFIALFKKKQNTTNPFPRS N DKSSRYR GDV N+ A+AC
Sbjct: 446 VFAGANHLLRTDPVEDKEGFFIALFKKKQNTTNPFPRSSNRDKSSRYRGGDVCNSWAVAC 505

Query: 481 PPTKISKLRLYQYALSTRTRWRKNHSIQN 508
           PPTKISKL LYQYALSTRTRWRKNH IQN
Sbjct: 506 PPTKISKLWLYQYALSTRTRWRKNHPIQN 534

BLAST of Cmc07g0182321 vs. ExPASy TrEMBL
Match: A0A6J1F919 (probable 28S rRNA (Cytosine-C(5))-methyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111443222 PE=3 SV=1)

HSP 1 Score: 845.9 bits (2184), Expect = 8.8e-242
Identity = 437/506 (86.36%), Postives = 467/506 (92.29%), Query Frame = 0

Query: 1   MGRVNNRTKVSPSRAAYKSENRKMSNADRSAYFARREAAKILRRVLEGDAQRRAIASIKS 60
           MGRVN RTK S +  A KSE+R+MSNADRSAYFARREAAKILRRVLEGDAQRRA+ASIKS
Sbjct: 1   MGRVNPRTKPSVAGTAGKSESRRMSNADRSAYFARREAAKILRRVLEGDAQRRAVASIKS 60

Query: 61  LVYAPSVRNKKGTFALVCKTLKYLPVIKDVLEAADLLSNKWKRQKELIYVIMYDILLGQK 120
           LVYAPSVRNKKGTFALVCKTLKYLP+IK+VLEAADLLSNKWKRQKELIYVIMYDILLGQK
Sbjct: 61  LVYAPSVRNKKGTFALVCKTLKYLPIIKEVLEAADLLSNKWKRQKELIYVIMYDILLGQK 120

Query: 121 AHLAGDAEKFLMRQQSALQSAVTQLLSKKKANSIEDLITRGDDDPGVSRPRFIRVNTLKM 180
             LAGDAEKFLMRQQSALQS VTQLLSK+KAN+IEDLI RG  +PGVSRPRFIRVNTLKM
Sbjct: 121 TPLAGDAEKFLMRQQSALQSVVTQLLSKRKANNIEDLIARG-HNPGVSRPRFIRVNTLKM 180

Query: 181 DVHTAIKELGKRYPVQKDDMVPDLLILPPGSDLHDHPLVADGSIFLQGKASSMVAVALDP 240
           DVHTAI+ELGKRY VQKDDMVPDLL+LPPGSDLHDHPLVADGSIF+QGKASSMVAVALDP
Sbjct: 181 DVHTAIQELGKRYAVQKDDMVPDLLVLPPGSDLHDHPLVADGSIFMQGKASSMVAVALDP 240

Query: 241 KPGWEVLDACSAPGNKTVHLASLMNGKGRVIACELNESRVKRLQHTIKLSGASN--VLHG 300
           KPGWEVLDACSAPGNKTVHLASLMNG+GR+IACELNE+RVKRLQHTIKLSGASN  VLHG
Sbjct: 241 KPGWEVLDACSAPGNKTVHLASLMNGEGRIIACELNENRVKRLQHTIKLSGASNIEVLHG 300

Query: 301 DFLKLSPKDPSLSKVRAILLDPSCSGSGTAAVRLDHLLPSHVEGTTSGDDLERLNKLAAF 360
           DFL L PKDPSLSKVRAILLDPSCSGSGTAA RLDHLLPSHVEG +SGDDLERL KLAAF
Sbjct: 301 DFLALDPKDPSLSKVRAILLDPSCSGSGTAAARLDHLLPSHVEGKSSGDDLERLEKLAAF 360

Query: 361 QRKALAHAFSFPAVERIVYSTCSIHQIENEDVVQSLLPLAESHGFQLDTPFPQWQRRGLP 420
           QRKALAHAFSFPAVERIVYSTCS+HQ+ENEDVVQSLLPLAESHGFQL+TPFPQWQRRG P
Sbjct: 361 QRKALAHAFSFPAVERIVYSTCSVHQVENEDVVQSLLPLAESHGFQLETPFPQWQRRGHP 420

Query: 421 VFAGANHLLRTDPVEDKEGFFIALFKKKQNTTNPFPRSLNIDKSSRYRSGDVRNNRALAC 480
           VFAGANHLLRTDP+ED EGFFIALF KKQNTT+PFP+  + DKSSR   G+VRN+RA+AC
Sbjct: 421 VFAGANHLLRTDPIEDTEGFFIALF-KKQNTTHPFPQISSRDKSSRNHGGNVRNSRAVAC 480

Query: 481 PPTKISKLRLYQYALSTRTRWRKNHS 505
            PT  S  +++ YA S RTR RK H+
Sbjct: 481 LPT--SMFKMWMYAHSKRTRRRKIHT 502

BLAST of Cmc07g0182321 vs. TAIR 10
Match: AT5G26180.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 596.3 bits (1536), Expect = 2.3e-170
Identity = 300/433 (69.28%), Postives = 354/433 (81.76%), Query Frame = 0

Query: 22  RKMSNADRSAYFARREAAKILRRVLEGDAQRRAIASIKSLVYAPSVRNKKGTFALVCKTL 81
           +K S+ +RSA +ARREAA ILR VL GDA+RRA+ASIKSLV +PSVRNK+GTFALVC+TL
Sbjct: 86  QKFSSLERSALYARREAANILRTVLRGDAERRAVASIKSLVLSPSVRNKRGTFALVCETL 145

Query: 82  KYLPVIKDVLEAADLLSNKWKRQKELIYVIMYDILLGQKAHLAGDAEKFLMRQQSALQSA 141
           KYL VIKDVL+ A++L++KWKRQ+ L++++ YDIL G+     GDAEKFLMR + AL S 
Sbjct: 146 KYLTVIKDVLDIANVLNSKWKRQEPLVFIVCYDILFGKDTPSIGDAEKFLMRHKEALLSG 205

Query: 142 VTQLLSKKKANSIEDLITRGDDDPGVSRPRFIRVNTLKMDVHTAIKELGKRYPVQKDDMV 201
           +  LL +KK +S++ L+  G    G  +PR++RVNTLKMDV +A++EL K Y VQKD+ V
Sbjct: 206 LATLLVRKKVDSVDQLL--GSKLTGHLKPRYVRVNTLKMDVDSAVQELEKHYTVQKDETV 265

Query: 202 PDLLILPPGSDLHDHPLVADGSIFLQGKASSMVAVALDPKPGWEVLDACSAPGNKTVHLA 261
           PDLL+LPPGSDLH H LVA+G IFLQGKASSMVA AL P+ GWEVLDACSAPGNKT+HLA
Sbjct: 266 PDLLVLPPGSDLHAHRLVANGRIFLQGKASSMVAAALQPQAGWEVLDACSAPGNKTIHLA 325

Query: 262 SLMNGKGRVIACELNESRVKRLQHTIKLSGASN--VLHGDFLKLSPKDPSLSKVRAILLD 321
           +LM G+G++IACELNE RVKRL+HTIKLSGASN  V HGDFL L+PKDPS +K+RAILLD
Sbjct: 326 ALMEGQGKIIACELNEERVKRLEHTIKLSGASNIEVCHGDFLGLNPKDPSFAKIRAILLD 385

Query: 322 PSCSGSGTAAVRLDHLLPSHVEGTTSGDDLERLNKLAAFQRKALAHAFSFPAVERIVYST 381
           PSCSGSGT   RLDHLLPSH E      D  RL+KLA FQ+KALAHA SFP VER+VYST
Sbjct: 386 PSCSGSGTITDRLDHLLPSHSEDNNMNYDSMRLHKLAVFQKKALAHALSFPKVERVVYST 445

Query: 382 CSIHQIENEDVVQSLLPLAESHGFQLDTPFPQWQRRGLPVFAGANHLLRTDPVEDKEGFF 441
           CSI+QIENEDVV S+LPLA S GF+L TPFPQWQRRGLPVFAG+ HLLR DPVEDKEGFF
Sbjct: 446 CSIYQIENEDVVSSVLPLASSLGFKLATPFPQWQRRGLPVFAGSEHLLRMDPVEDKEGFF 505

Query: 442 IALFKKKQNTTNP 453
           IALF +     NP
Sbjct: 506 IALFVRANKLDNP 516

BLAST of Cmc07g0182321 vs. TAIR 10
Match: AT5G26180.2 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 596.3 bits (1536), Expect = 2.3e-170
Identity = 300/433 (69.28%), Postives = 354/433 (81.76%), Query Frame = 0

Query: 22  RKMSNADRSAYFARREAAKILRRVLEGDAQRRAIASIKSLVYAPSVRNKKGTFALVCKTL 81
           +K S+ +RSA +ARREAA ILR VL GDA+RRA+ASIKSLV +PSVRNK+GTFALVC+TL
Sbjct: 86  QKFSSLERSALYARREAANILRTVLRGDAERRAVASIKSLVLSPSVRNKRGTFALVCETL 145

Query: 82  KYLPVIKDVLEAADLLSNKWKRQKELIYVIMYDILLGQKAHLAGDAEKFLMRQQSALQSA 141
           KYL VIKDVL+ A++L++KWKRQ+ L++++ YDIL G+     GDAEKFLMR + AL S 
Sbjct: 146 KYLTVIKDVLDIANVLNSKWKRQEPLVFIVCYDILFGKDTPSIGDAEKFLMRHKEALLSG 205

Query: 142 VTQLLSKKKANSIEDLITRGDDDPGVSRPRFIRVNTLKMDVHTAIKELGKRYPVQKDDMV 201
           +  LL +KK +S++ L+  G    G  +PR++RVNTLKMDV +A++EL K Y VQKD+ V
Sbjct: 206 LATLLVRKKVDSVDQLL--GSKLTGHLKPRYVRVNTLKMDVDSAVQELEKHYTVQKDETV 265

Query: 202 PDLLILPPGSDLHDHPLVADGSIFLQGKASSMVAVALDPKPGWEVLDACSAPGNKTVHLA 261
           PDLL+LPPGSDLH H LVA+G IFLQGKASSMVA AL P+ GWEVLDACSAPGNKT+HLA
Sbjct: 266 PDLLVLPPGSDLHAHRLVANGRIFLQGKASSMVAAALQPQAGWEVLDACSAPGNKTIHLA 325

Query: 262 SLMNGKGRVIACELNESRVKRLQHTIKLSGASN--VLHGDFLKLSPKDPSLSKVRAILLD 321
           +LM G+G++IACELNE RVKRL+HTIKLSGASN  V HGDFL L+PKDPS +K+RAILLD
Sbjct: 326 ALMEGQGKIIACELNEERVKRLEHTIKLSGASNIEVCHGDFLGLNPKDPSFAKIRAILLD 385

Query: 322 PSCSGSGTAAVRLDHLLPSHVEGTTSGDDLERLNKLAAFQRKALAHAFSFPAVERIVYST 381
           PSCSGSGT   RLDHLLPSH E      D  RL+KLA FQ+KALAHA SFP VER+VYST
Sbjct: 386 PSCSGSGTITDRLDHLLPSHSEDNNMNYDSMRLHKLAVFQKKALAHALSFPKVERVVYST 445

Query: 382 CSIHQIENEDVVQSLLPLAESHGFQLDTPFPQWQRRGLPVFAGANHLLRTDPVEDKEGFF 441
           CSI+QIENEDVV S+LPLA S GF+L TPFPQWQRRGLPVFAG+ HLLR DPVEDKEGFF
Sbjct: 446 CSIYQIENEDVVSSVLPLASSLGFKLATPFPQWQRRGLPVFAGSEHLLRMDPVEDKEGFF 505

Query: 442 IALFKKKQNTTNP 453
           IALF +     NP
Sbjct: 506 IALFVRANKLDNP 516

BLAST of Cmc07g0182321 vs. TAIR 10
Match: AT3G13180.1 (NOL1/NOP2/sun family protein / antitermination NusB domain-containing protein )

HSP 1 Score: 84.7 bits (208), Expect = 2.3e-16
Identity = 54/181 (29.83%), Postives = 92/181 (50.83%), Query Frame = 0

Query: 218 LVADGSIFLQGKASSMVAVALDPKPGWEVLDACSAPGNKTVHLASLMNGKGRVIACELNE 277
           L+ +G   +Q +++ ++   + P+PG  ++DAC+APG KT+ +AS + G+G + A ++NE
Sbjct: 306 LLKEGICSVQDESAGLIVSVVKPQPGERIMDACAAPGGKTLFMASCLKGQGMIYAMDVNE 365

Query: 278 SRVKRLQHTIK---LSGASNVLHGDFLKLSPKDPSLSKVRAILLDPSCSGSGTAAVRLDH 337
            R++ L  T K   + G    +H D    +  + +  +   +LLD  CSG G  + R D 
Sbjct: 366 GRLRILGETAKSHQVDGLITTIHSDLRVFA--ETNEVQYDKVLLDAPCSGLGVLSKRAD- 425

Query: 338 LLPSHVEGTTSGDDLERLNKLAAFQRKALAHAFSFPAVERIVYSTCSIHQIENEDVVQSL 396
                +      +D+  L KL      + +       V  +VYSTCSI   ENE  V++ 
Sbjct: 426 -----LRWNRKLEDMLELTKLQDELLDSASKLVKHGGV--LVYSTCSIDPEENEGRVEAF 476

BLAST of Cmc07g0182321 vs. TAIR 10
Match: AT1G06560.1 (NOL1/NOP2/sun family protein )

HSP 1 Score: 63.9 bits (154), Expect = 4.2e-10
Identity = 29/67 (43.28%), Postives = 45/67 (67.16%), Query Frame = 0

Query: 219 VADGSIFLQGKASSMVAVALDPKPGWEVLDACSAPGNKTVHLASLMNGKGRVIACELNES 278
           + +G IFLQ   S +VA ALDP+ G  +LD C+APG KT  +A LMN +G ++A + + +
Sbjct: 273 ILEGEIFLQNLPSIIVAHALDPQKGERILDMCAAPGGKTTAIAILMNDEGEIVAADRSHN 332

Query: 279 RVKRLQH 286
           +V  +Q+
Sbjct: 333 KVLVVQN 339

BLAST of Cmc07g0182321 vs. TAIR 10
Match: AT4G40000.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 53.5 bits (127), Expect = 5.7e-07
Identity = 54/199 (27.14%), Postives = 83/199 (41.71%), Query Frame = 0

Query: 222 GSIFLQGKASSMVAVALDPKPGWEVLDACSAPGNKTVHLASLMN--------GKGRVIAC 281
           G++  Q   S +  + LD  P   VLD C+APG+KT  L  +++          G V+A 
Sbjct: 158 GNMTRQESVSMVPPLFLDVHPDHFVLDMCAAPGSKTFQLLEIIHESSEPGSLPNGMVVAN 217

Query: 282 ELNESRVKRLQHTIKLSGASNVL----HGDFLKLSPKDPSLSKVR-------------AI 341
           +++  R   L H  K +  +N++     G          +LS                 +
Sbjct: 218 DVDYKRSNLLIHQTKRTCTTNLMVTNNEGQHFPSCNTKRTLSVASETNPHPIDQLLFDRV 277

Query: 342 LLDPSCSGSGTAAVRLDHLLPSHVEGTTSGDDLERLNKLAAFQRKALAHAFSFPAVERIV 396
           L D  CSG GT     D         + SG+ L  L  + A +  +L          R+V
Sbjct: 278 LCDVPCSGDGTLRKAPDIW---RRWNSGSGNGLHSLQVVLAMRGLSLLKVGG-----RMV 337

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_008452765.12.7e-28599.21PREDICTED: probable 28S rRNA (cytosine-C(5))-methyltransferase isoform X2 [Cucum... [more]
XP_008452764.13.3e-28398.62PREDICTED: probable 28S rRNA (cytosine-C(5))-methyltransferase isoform X1 [Cucum... [more]
XP_004141333.24.5e-26492.34probable 28S rRNA (cytosine-C(5))-methyltransferase [Cucumis sativus][more]
KAE8649915.14.5e-26492.34hypothetical protein Csa_012918 [Cucumis sativus][more]
XP_038898177.13.9e-26091.1228S rRNA (cytosine-C(5))-methyltransferase-like isoform X1 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
Q8GYE83.3e-16969.2825S rRNA (cytosine-C(5))-methyltransferase NSUN5 OS=Arabidopsis thaliana OX=3702... [more]
Q8K4F66.0e-7039.0028S rRNA (cytosine-C(5))-methyltransferase OS=Mus musculus OX=10090 GN=Nsun5 PE=... [more]
Q96P115.1e-6939.2928S rRNA (cytosine-C(5))-methyltransferase OS=Homo sapiens OX=9606 GN=NSUN5 PE=1... [more]
O140391.7e-5333.4125S rRNA (cytosine-C(5))-methyltransferase rcm1 OS=Schizosaccharomyces pombe (st... [more]
Q9NAA77.6e-4931.8426S rRNA (cytosine-C(5))-methyltransferase nsun-5 OS=Caenorhabditis elegans OX=6... [more]
Match NameE-valueIdentityDescription
A0A1S3BVF01.3e-28599.21probable 28S rRNA (Cytosine-C(5))-methyltransferase isoform X2 OS=Cucumis melo O... [more]
A0A5D3D9A21.6e-28398.62Putative 28S rRNA (Cytosine-C(5))-methyltransferase isoform X1 OS=Cucumis melo v... [more]
A0A1S3BUJ81.6e-28398.62probable 28S rRNA (Cytosine-C(5))-methyltransferase isoform X1 OS=Cucumis melo O... [more]
A0A0A0L2U22.2e-26492.34SAM_MT_RSMB_NOP domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G64... [more]
A0A6J1F9198.8e-24286.36probable 28S rRNA (Cytosine-C(5))-methyltransferase OS=Cucurbita moschata OX=366... [more]
Match NameE-valueIdentityDescription
AT5G26180.12.3e-17069.28S-adenosyl-L-methionine-dependent methyltransferases superfamily protein [more]
AT5G26180.22.3e-17069.28S-adenosyl-L-methionine-dependent methyltransferases superfamily protein [more]
AT3G13180.12.3e-1629.83NOL1/NOP2/sun family protein / antitermination NusB domain-containing protein [more]
AT1G06560.14.2e-1043.28NOL1/NOP2/sun family protein [more]
AT4G40000.15.7e-0727.14S-adenosyl-L-methionine-dependent methyltransferases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Melon (Charmono) v1.1
Date Performed: 2022-10-13
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR023267RNA (C5-cytosine) methyltransferasePRINTSPR02008RCMTFAMILYcoord: 314..326
score: 52.08
coord: 372..388
score: 46.95
coord: 247..257
score: 67.26
coord: 218..232
score: 24.7
coord: 428..445
score: 30.24
NoneNo IPR availableGENE3D3.30.70.1170Sun protein; domain 3coord: 161..226
e-value: 2.0E-11
score: 45.9
NoneNo IPR availableGENE3D3.40.50.150Vaccinia Virus protein VP39coord: 227..452
e-value: 1.3E-66
score: 226.0
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..23
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..20
NoneNo IPR availablePANTHERPTHR22807:SF66BNAA09G04380D PROTEINcoord: 11..467
NoneNo IPR availablePANTHERPTHR22807NOP2 YEAST -RELATED NOL1/NOP2/FMU SUN DOMAIN-CONTAININGcoord: 11..467
NoneNo IPR availableCDDcd02440AdoMet_MTasescoord: 246..332
e-value: 1.32534E-6
score: 45.1135
IPR001678SAM-dependent methyltransferase RsmB/NOP2-typePFAMPF01189Methyltr_RsmB-Fcoord: 236..444
e-value: 1.5E-24
score: 86.8
IPR001678SAM-dependent methyltransferase RsmB/NOP2-typePROSITEPS51686SAM_MT_RSMB_NOPcoord: 161..446
score: 47.562634
IPR029063S-adenosyl-L-methionine-dependent methyltransferaseSUPERFAMILY53335S-adenosyl-L-methionine-dependent methyltransferasescoord: 184..446

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cmc07g0182321.1Cmc07g0182321.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0001510 RNA methylation
molecular_function GO:0008168 methyltransferase activity
molecular_function GO:0003729 mRNA binding