Cmc06g0163481 (gene) Melon (Charmono) v1.1

BLAST of Cmc06g0163481 vs. NCBI nr
Match: XP_008457115.1 (PREDICTED: nicastrin isoform X1 [Cucumis melo])

HSP 1 Score: 1338.6 bits (3463), Expect = 0.0e+00
Identity = 683/692 (98.70%), Postives = 683/692 (98.70%), Query Frame = 0

Query: 1   MNDPTDSDLIERTPKSSSMSSQFLYLLLFLTSLRLSSSDEQKMESVPDLQNSMYLAVDGY 60
           MNDPTDSDLIERTPKSSSMSSQFLYLLLFLTSLRLSSSDEQKMESVPDLQNSMYLAVDGY
Sbjct: 1   MNDPTDSDLIERTPKSSSMSSQFLYLLLFLTSLRLSSSDEQKMESVPDLQNSMYLAVDGY 60

Query: 61  PCIRLLNLSGEIGCSNPGREKVVLPMINFKDADEILEPSAVLVSMDAISSFFTRLQDDSH 120
           PCIRLLNLSGEIGCSNPGREKVVLPMINFKDADEILEPSAVLVSMDAISSFFTRLQDDSH
Sbjct: 61  PCIRLLNLSGEIGCSNPGREKVVLPMINFKDADEILEPSAVLVSMDAISSFFTRLQDDSH 120

Query: 121 FANNVGGVLIEPGTGIQNRTEGFSPAQKFPQAKFAPYKKNDYEWNPIGSGIMWNRYNFPV 180
           FANNVGGVLIEPGTGIQNRTEGFSPAQKFPQAKFAPYKKNDYEWNPIGSGIMWNRYNFPV
Sbjct: 121 FANNVGGVLIEPGTGIQNRTEGFSPAQKFPQAKFAPYKKNDYEWNPIGSGIMWNRYNFPV 180

Query: 181 FLISESSISSIQEAASKNVKSKKDYVSNVAEFDLVMQTTKAGTHSSMSCLKEETCLPLGG 240
           FLISESSISSIQEAASKNVKSKKDYVSNVAEFDLVMQTTKAGTHSSMSCLKEETCLPLGG
Sbjct: 181 FLISESSISSIQEAASKNVKSKKDYVSNVAEFDLVMQTTKAGTHSSMSCLKEETCLPLGG 240

Query: 241 YSVWSSLPPINTSSSDQSKPVILTVASMDSASFFRDKSIGADSPISGLIALLAAVDALSH 300
           YSVWSSLPPINTSSSDQSKPVILTVASMDSASFFRDKSIGADSPISGLIALLAAVDALSH
Sbjct: 241 YSVWSSLPPINTSSSDQSKPVILTVASMDSASFFRDKSIGADSPISGLIALLAAVDALSH 300

Query: 301 VDGLDDLHKQLVFVVFTGESWGYLGSRRFLLELDLQSDAVSGLSNRLIDMEELVVVLRQV 360
           VDGLDDLHKQLVFVVFTGESWGYLGSRRFLLELDLQSDAVSGLSNRLIDM         V
Sbjct: 301 VDGLDDLHKQLVFVVFTGESWGYLGSRRFLLELDLQSDAVSGLSNRLIDM---------V 360

Query: 361 FEIGSVGKSSNHGSGNFFAHMTEVSSSKNETWNALKLARESLPLENIKVSPASTTNPGIP 420
           FEIGSVGKSSNHGSGNFFAHMTEVSSSKNETWNALKLARESLPLENIKVSPASTTNPGIP
Sbjct: 361 FEIGSVGKSSNHGSGNFFAHMTEVSSSKNETWNALKLARESLPLENIKVSPASTTNPGIP 420

Query: 421 PSSLMAFLAKNPQISGVVLDDFDTGFTNQFYQSHLDDLHNINSSAIEAAALLVARTLYIL 480
           PSSLMAFLAKNPQISGVVLDDFDTGFTNQFYQSHLDDLHNINSSAIEAAALLVARTLYIL
Sbjct: 421 PSSLMAFLAKNPQISGVVLDDFDTGFTNQFYQSHLDDLHNINSSAIEAAALLVARTLYIL 480

Query: 481 AINKNELSSSVLTAIKVNTSLVEELIGCLLNCDPGLSCELVKRYITPSSVCPNHYVGVIL 540
           AINKNELSSSVLTAIKVNTSLVEELIGCLLNCDPGLSCELVKRYITPSSVCPNHYVGVIL
Sbjct: 481 AINKNELSSSVLTAIKVNTSLVEELIGCLLNCDPGLSCELVKRYITPSSVCPNHYVGVIL 540

Query: 541 DEPSSAPYPDYVHDVSRFVWNFLADRTSIPKENTSSVCSQNCDDRSEVCIGAETGKGTCV 600
           DEPSSAPYPDYVHDVSRFVWNFLADRTSIPKENTSSVCSQNCDDRSEVCIGAETGKGTCV
Sbjct: 541 DEPSSAPYPDYVHDVSRFVWNFLADRTSIPKENTSSVCSQNCDDRSEVCIGAETGKGTCV 600

Query: 601 ISTTRYIPAYSTRLKFESGYWSVLPPNSSDHLGAVDPVWTESNWNTIGLRIYTIQAAAYD 660
           ISTTRYIPAYSTRLKFESGYWSVLPPNSSDHLGAVDPVWTESNWNTIGLRIYTIQAAAYD
Sbjct: 601 ISTTRYIPAYSTRLKFESGYWSVLPPNSSDHLGAVDPVWTESNWNTIGLRIYTIQAAAYD 660

Query: 661 RFVLLGGITTTILAYFAIVAVRSSIIKALKRD 693
           RFVLLGGITTTILAYFAIVAVRSSIIKALKRD
Sbjct: 661 RFVLLGGITTTILAYFAIVAVRSSIIKALKRD 683

BLAST of Cmc06g0163481 vs. NCBI nr
Match: XP_008457117.1 (PREDICTED: nicastrin isoform X2 [Cucumis melo])

HSP 1 Score: 1281.5 bits (3315), Expect = 0.0e+00
Identity = 659/692 (95.23%), Postives = 659/692 (95.23%), Query Frame = 0

Query: 1   MNDPTDSDLIERTPKSSSMSSQFLYLLLFLTSLRLSSSDEQKMESVPDLQNSMYLAVDGY 60
           MNDPTDSDLIERTPKSSSMSSQFLYLLLFLTSLRLSSSDEQKMESVPDLQNSMYLAVDGY
Sbjct: 1   MNDPTDSDLIERTPKSSSMSSQFLYLLLFLTSLRLSSSDEQKMESVPDLQNSMYLAVDGY 60

Query: 61  PCIRLLNLSGEIGCSNPGREKVVLPMINFKDADEILEPSAVLVSMDAISSFFTRLQDDSH 120
           PCIRLLNLSGEIGCSNPGREKVVLPMINFKDADEILEPSAVLVSMDAISSFFTRLQDDSH
Sbjct: 61  PCIRLLNLSGEIGCSNPGREKVVLPMINFKDADEILEPSAVLVSMDAISSFFTRLQDDSH 120

Query: 121 FANNVGGVLIEPGTGIQNRTEGFSPAQKFPQAKFAPYKKNDYEWNPIGSGIMWNRYNFPV 180
           FANNVGGVLIEPGTGIQNRTEGFSPAQKFPQAKFAPYKKNDYEWNPIGSGIMWNRYNFPV
Sbjct: 121 FANNVGGVLIEPGTGIQNRTEGFSPAQKFPQAKFAPYKKNDYEWNPIGSGIMWNRYNFPV 180

Query: 181 FLISESSISSIQEAASKNVKSKKDYVSNVAEFDLVMQTTKAGTHSSMSCLKEETCLPLGG 240
           FLISESSISSIQEAASKNVKSKKDYVSNVAEFDLVMQTTKAGTHSSMSCLKEETCLPLGG
Sbjct: 181 FLISESSISSIQEAASKNVKSKKDYVSNVAEFDLVMQTTKAGTHSSMSCLKEETCLPLGG 240

Query: 241 YSVWSSLPPINTSSSDQSKPVILTVASMDSASFFRDKSIGADSPISGLIALLAAVDALSH 300
           YSVWSSLPPINTSSSDQSKPVILTVASMDSASFFRDKSIGADSPISGLIALLAAVDALSH
Sbjct: 241 YSVWSSLPPINTSSSDQSKPVILTVASMDSASFFRDKSIGADSPISGLIALLAAVDALSH 300

Query: 301 VDGLDDLHKQLVFVVFTGESWGYLGSRRFLLELDLQSDAVSGLSNRLIDMEELVVVLRQV 360
           VDGLDDLHKQLVFVVFTGESWGYLGSRRFLLELDLQSDAVSGLSNRLIDM          
Sbjct: 301 VDGLDDLHKQLVFVVFTGESWGYLGSRRFLLELDLQSDAVSGLSNRLIDM---------- 360

Query: 361 FEIGSVGKSSNHGSGNFFAHMTEVSSSKNETWNALKLARESLPLENIKVSPASTTNPGIP 420
                                  VSSSKNETWNALKLARESLPLENIKVSPASTTNPGIP
Sbjct: 361 -----------------------VSSSKNETWNALKLARESLPLENIKVSPASTTNPGIP 420

Query: 421 PSSLMAFLAKNPQISGVVLDDFDTGFTNQFYQSHLDDLHNINSSAIEAAALLVARTLYIL 480
           PSSLMAFLAKNPQISGVVLDDFDTGFTNQFYQSHLDDLHNINSSAIEAAALLVARTLYIL
Sbjct: 421 PSSLMAFLAKNPQISGVVLDDFDTGFTNQFYQSHLDDLHNINSSAIEAAALLVARTLYIL 480

Query: 481 AINKNELSSSVLTAIKVNTSLVEELIGCLLNCDPGLSCELVKRYITPSSVCPNHYVGVIL 540
           AINKNELSSSVLTAIKVNTSLVEELIGCLLNCDPGLSCELVKRYITPSSVCPNHYVGVIL
Sbjct: 481 AINKNELSSSVLTAIKVNTSLVEELIGCLLNCDPGLSCELVKRYITPSSVCPNHYVGVIL 540

Query: 541 DEPSSAPYPDYVHDVSRFVWNFLADRTSIPKENTSSVCSQNCDDRSEVCIGAETGKGTCV 600
           DEPSSAPYPDYVHDVSRFVWNFLADRTSIPKENTSSVCSQNCDDRSEVCIGAETGKGTCV
Sbjct: 541 DEPSSAPYPDYVHDVSRFVWNFLADRTSIPKENTSSVCSQNCDDRSEVCIGAETGKGTCV 600

Query: 601 ISTTRYIPAYSTRLKFESGYWSVLPPNSSDHLGAVDPVWTESNWNTIGLRIYTIQAAAYD 660
           ISTTRYIPAYSTRLKFESGYWSVLPPNSSDHLGAVDPVWTESNWNTIGLRIYTIQAAAYD
Sbjct: 601 ISTTRYIPAYSTRLKFESGYWSVLPPNSSDHLGAVDPVWTESNWNTIGLRIYTIQAAAYD 659

Query: 661 RFVLLGGITTTILAYFAIVAVRSSIIKALKRD 693
           RFVLLGGITTTILAYFAIVAVRSSIIKALKRD
Sbjct: 661 RFVLLGGITTTILAYFAIVAVRSSIIKALKRD 659

BLAST of Cmc06g0163481 vs. NCBI nr
Match: XP_004140732.1 (nicastrin [Cucumis sativus] >KGN57470.2 hypothetical protein Csa_011284 [Cucumis sativus])

HSP 1 Score: 1263.1 bits (3267), Expect = 0.0e+00
Identity = 640/674 (94.96%), Postives = 652/674 (96.74%), Query Frame = 0

Query: 19  MSSQFLYLLLFLTSLRLSSSDEQKMESVPDLQNSMYLAVDGYPCIRLLNLSGEIGCSNPG 78
           MSSQFLYLLLFLTSL LSSSDE  MESVPDLQNSMYLAVD YPCIRLLNLSGEIGCSNPG
Sbjct: 1   MSSQFLYLLLFLTSLCLSSSDEHSMESVPDLQNSMYLAVDAYPCIRLLNLSGEIGCSNPG 60

Query: 79  REKVVLPMINFKDADEILEPSAVLVSMDAISSFFTRLQDDSHFANNVGGVLIEPGTGIQN 138
           REKVV+PMINFKDADEIL+PSAVLVSMDAISSFFTRLQDDSHFANNVGGVLIEPGTGIQN
Sbjct: 61  REKVVVPMINFKDADEILQPSAVLVSMDAISSFFTRLQDDSHFANNVGGVLIEPGTGIQN 120

Query: 139 RTEGFSPAQKFPQAKFAPYKKNDYEWNPIGSGIMWNRYNFPVFLISESSISSIQEAASKN 198
           RTEGFSPAQKFPQAKFAPY+K+DYEWNP GSGIMWN+YNFPVFLISESSISSIQEAASKN
Sbjct: 121 RTEGFSPAQKFPQAKFAPYEKSDYEWNPSGSGIMWNQYNFPVFLISESSISSIQEAASKN 180

Query: 199 VKSKKDYVSNVAEFDLVMQTTKAGTHSSMSCLKEETCLPLGGYSVWSSLPPINTSSSDQS 258
           VKSKKDY+SNVAEFDLVMQTTKAGTHSSMSCLKEETCLPLGGYSVWSSLPPINTSSSDQS
Sbjct: 181 VKSKKDYISNVAEFDLVMQTTKAGTHSSMSCLKEETCLPLGGYSVWSSLPPINTSSSDQS 240

Query: 259 KPVILTVASMDSASFFRDKSIGADSPISGLIALLAAVDALSHVDGLDDLHKQLVFVVFTG 318
           KPVILTVASMDSASFFRDKSIGADSPISGLIALLAAVDALSHVDGLDDLHKQLVF VFTG
Sbjct: 241 KPVILTVASMDSASFFRDKSIGADSPISGLIALLAAVDALSHVDGLDDLHKQLVFAVFTG 300

Query: 319 ESWGYLGSRRFLLELDLQSDAVSGLSNRLIDMEELVVVLRQVFEIGSVGKSSNHGSGNFF 378
           ESWGYLGSRRFLLELDLQSDAVSGL NRLID          VFEIGSVGKSS HGSGNFF
Sbjct: 301 ESWGYLGSRRFLLELDLQSDAVSGLENRLID---------TVFEIGSVGKSSKHGSGNFF 360

Query: 379 AHMTEVSSSKNETWNALKLARESLPLENIKVSPASTTNPGIPPSSLMAFLAKNPQISGVV 438
           AHMTEVSSS NETWNALKLARESLPLENIKVSPASTTNPGIPPSSLMAFLAKNPQ+SGVV
Sbjct: 361 AHMTEVSSSNNETWNALKLARESLPLENIKVSPASTTNPGIPPSSLMAFLAKNPQVSGVV 420

Query: 439 LDDFDTGFTNQFYQSHLDDLHNINSSAIEAAALLVARTLYILAINKNELSSSVLTAIKVN 498
           L+DFDTGFTNQFYQS+LDDLHNINSSAIEAAALLVARTLYILAINK ELSSSVLTAIKVN
Sbjct: 421 LEDFDTGFTNQFYQSYLDDLHNINSSAIEAAALLVARTLYILAINKKELSSSVLTAIKVN 480

Query: 499 TSLVEELIGCLLNCDPGLSCELVKRYITPSSVCPNHYVGVILDEPSSAPYPDYVHDVSRF 558
           TSLVEELIGCLLNCDPGLSCELVKRYI+PSSVCPNHYVGVILDEPSSAPYPDYVHDVSRF
Sbjct: 481 TSLVEELIGCLLNCDPGLSCELVKRYISPSSVCPNHYVGVILDEPSSAPYPDYVHDVSRF 540

Query: 559 VWNFLADRTSIPKENTSSVCSQNCDDRSEVCIGAETGKGTCVISTTRYIPAYSTRLKFES 618
           VWNFLADRTSIPKENTSSVCSQNCDD+SEVCIGAETGKGTC ISTTRYIPAYSTRLKFES
Sbjct: 541 VWNFLADRTSIPKENTSSVCSQNCDDKSEVCIGAETGKGTCAISTTRYIPAYSTRLKFES 600

Query: 619 GYWSVLPPNSSDHLGAVDPVWTESNWNTIGLRIYTIQAAAYDRFVLLGGITTTILAYFAI 678
           GYWSVLPPNSSDHLG VDPVWTESNWNTIGLR+YTIQAAAYDRFVLLGGITTTILAYFAI
Sbjct: 601 GYWSVLPPNSSDHLGTVDPVWTESNWNTIGLRVYTIQAAAYDRFVLLGGITTTILAYFAI 660

Query: 679 VAVRSSIIKALKRD 693
           VAVRSSIIKALKRD
Sbjct: 661 VAVRSSIIKALKRD 665

BLAST of Cmc06g0163481 vs. NCBI nr
Match: XP_038896057.1 (nicastrin [Benincasa hispida])

HSP 1 Score: 1234.6 bits (3193), Expect = 0.0e+00
Identity = 626/674 (92.88%), Postives = 644/674 (95.55%), Query Frame = 0

Query: 19  MSSQFLYLLLFLTSLRLSSSDEQKMESVPDLQNSMYLAVDGYPCIRLLNLSGEIGCSNPG 78
           MSS FLYLLLF+TSLRLSSSDE  MESVPDLQNSMYLAVDGYPCIRLLNLSGEIGCSNPG
Sbjct: 1   MSSHFLYLLLFVTSLRLSSSDEHSMESVPDLQNSMYLAVDGYPCIRLLNLSGEIGCSNPG 60

Query: 79  REKVVLPMINFKDADEILEPSAVLVSMDAISSFFTRLQDDSHFANNVGGVLIEPGTGIQN 138
           REKVV+PMINFKDADEIL+PSAVLVSMD ISSFFTRLQDDSHFANNVGGVLI+PGT +QN
Sbjct: 61  REKVVVPMINFKDADEILQPSAVLVSMDEISSFFTRLQDDSHFANNVGGVLIKPGTEMQN 120

Query: 139 RTEGFSPAQKFPQAKFAPYKKNDYEWNPIGSGIMWNRYNFPVFLISESSISSIQEAASKN 198
            T+GFSPAQKFPQA FAPYKK DYEWNPIGSGIMWN+YNFPVFLISESSISSIQEAASKN
Sbjct: 121 STKGFSPAQKFPQASFAPYKKIDYEWNPIGSGIMWNQYNFPVFLISESSISSIQEAASKN 180

Query: 199 VKSKKDYVSNVAEFDLVMQTTKAGTHSSMSCLKEETCLPLGGYSVWSSLPPINTSSSDQS 258
           VK+KKDY+SNVAEFDLVMQTTKAGTH+SMSCLKE TCLPLGGYSVWSSLPPINTSSSDQS
Sbjct: 181 VKNKKDYISNVAEFDLVMQTTKAGTHNSMSCLKEVTCLPLGGYSVWSSLPPINTSSSDQS 240

Query: 259 KPVILTVASMDSASFFRDKSIGADSPISGLIALLAAVDALSHVDGLDDLHKQLVFVVFTG 318
           KPVILTVASMDSASFFRDKSIGADSPISGLIALLAAVDALSHVDG DDLHKQLVFVVFTG
Sbjct: 241 KPVILTVASMDSASFFRDKSIGADSPISGLIALLAAVDALSHVDGFDDLHKQLVFVVFTG 300

Query: 319 ESWGYLGSRRFLLELDLQSDAVSGLSNRLIDMEELVVVLRQVFEIGSVGKSSNHGSGNFF 378
           ESWGYLGSRRFLLELDLQSDAVSGL+NRLID          VFEIGSVGKSS+HG G FF
Sbjct: 301 ESWGYLGSRRFLLELDLQSDAVSGLNNRLID---------SVFEIGSVGKSSSHGFGKFF 360

Query: 379 AHMTEVSSSKNETWNALKLARESLPLENIKVSPASTTNPGIPPSSLMAFLAKNPQISGVV 438
           AHMTEVSSSKNETWNALKLARESLPLENIKVSPASTTNPGIPPSSLMAFLAKNPQISGVV
Sbjct: 361 AHMTEVSSSKNETWNALKLARESLPLENIKVSPASTTNPGIPPSSLMAFLAKNPQISGVV 420

Query: 439 LDDFDTGFTNQFYQSHLDDLHNINSSAIEAAALLVARTLYILAINKNELSSSVLTAIKVN 498
           L+DFDT FTNQFYQSHLDDLHNINSSAIEAAALLVARTLYILA NK ELSSS LTAIKVN
Sbjct: 421 LEDFDTSFTNQFYQSHLDDLHNINSSAIEAAALLVARTLYILATNKKELSSSALTAIKVN 480

Query: 499 TSLVEELIGCLLNCDPGLSCELVKRYITPSSVCPNHYVGVILDEPSSAPYPDYVHDVSRF 558
           TSLVEELIGCLLNCDPGLSCELVKRYI+PSSVCPNHYVGVILDEPSS PYP YVHDVSRF
Sbjct: 481 TSLVEELIGCLLNCDPGLSCELVKRYISPSSVCPNHYVGVILDEPSSTPYPGYVHDVSRF 540

Query: 559 VWNFLADRTSIPKENTSSVCSQNCDDRSEVCIGAETGKGTCVISTTRYIPAYSTRLKFES 618
           VWNFLADRTSIPKENTSSVCSQNCDD+SEVCIGAETGKGTCVISTTRYIPAYSTRLKFES
Sbjct: 541 VWNFLADRTSIPKENTSSVCSQNCDDKSEVCIGAETGKGTCVISTTRYIPAYSTRLKFES 600

Query: 619 GYWSVLPPNSSDHLGAVDPVWTESNWNTIGLRIYTIQAAAYDRFVLLGGITTTILAYFAI 678
           GYW+VLPPNSSD LGAVDPVWTESNWNTIGLR+YTIQA AYDRFVLLGGITTTILAYFAI
Sbjct: 601 GYWNVLPPNSSDPLGAVDPVWTESNWNTIGLRMYTIQATAYDRFVLLGGITTTILAYFAI 660

Query: 679 VAVRSSIIKALKRD 693
           VAV+SSIIKALKRD
Sbjct: 661 VAVQSSIIKALKRD 665

BLAST of Cmc06g0163481 vs. NCBI nr
Match: KAG6606989.1 (Nicastrin, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1172.9 bits (3033), Expect = 0.0e+00
Identity = 594/691 (85.96%), Postives = 633/691 (91.61%), Query Frame = 0

Query: 2   NDPTDSDLIERTPKSSSMSSQFLYLLLFLTSLRLSSSDEQKMESVPDLQNSMYLAVDGYP 61
           N P D D  +R  KS+SMS   L+LLLFLTS+RL  SDE  MESVPDLQNSMYL VDGYP
Sbjct: 29  NAPIDCDSNQRIAKSASMSFHILHLLLFLTSVRLCLSDEHSMESVPDLQNSMYLVVDGYP 88

Query: 62  CIRLLNLSGEIGCSNPGREKVVLPMINFKDADEILEPSAVLVSMDAISSFFTRLQDDSHF 121
           CIRLLNLSGEIGCSNPGREKVV+PMINFKDADEIL+PSA+LVSMD ISSFF RLQDDS+F
Sbjct: 89  CIRLLNLSGEIGCSNPGREKVVVPMINFKDADEILQPSAILVSMDDISSFFARLQDDSNF 148

Query: 122 ANNVGGVLIEPGTGIQNRTEGFSPAQKFPQAKFAPYKKNDYEWNPIGSGIMWNRYNFPVF 181
           A+NVGGVLI+PGT IQ RT+GFSPAQKFPQAKFAPY+K DYEWNPIGSG+MWN+YNFPVF
Sbjct: 149 ASNVGGVLIKPGTEIQKRTKGFSPAQKFPQAKFAPYQKIDYEWNPIGSGVMWNQYNFPVF 208

Query: 182 LISESSISSIQEAASKNVKSKKDYVSNVAEFDLVMQTTKAGTHSSMSCLKEETCLPLGGY 241
           LISESSIS +QEAASKNVK KK Y SNVAEFDLVMQTTKAGT +SMSCLKEETCLPLGGY
Sbjct: 209 LISESSISPVQEAASKNVKDKKIYTSNVAEFDLVMQTTKAGTRNSMSCLKEETCLPLGGY 268

Query: 242 SVWSSLPPINTSSSDQSKPVILTVASMDSASFFRDKSIGADSPISGLIALLAAVDALSHV 301
           SVWSSLPPIN  SSD+SKP+ILTVASMDSASFFRDKSIGADSPISGLIALLAAVDALSHV
Sbjct: 269 SVWSSLPPINI-SSDKSKPIILTVASMDSASFFRDKSIGADSPISGLIALLAAVDALSHV 328

Query: 302 DGLDDLHKQLVFVVFTGESWGYLGSRRFLLELDLQSDAVSGLSNRLIDMEELVVVLRQVF 361
           DGLDDLHKQLVFVVFTGESWGYLGSRRFLLELDLQSD+VSGL+N LID          VF
Sbjct: 329 DGLDDLHKQLVFVVFTGESWGYLGSRRFLLELDLQSDSVSGLNNTLID---------TVF 388

Query: 362 EIGSVGKSSNHGSGNFFAHMTEVSSSKNETWNALKLARESLPLENIKVSPASTTNPGIPP 421
           EIGSVGK SN G GNFFAHMTEVSSSKNETWNALKLA+ESLP ENIKVSPASTTNPGIPP
Sbjct: 389 EIGSVGKKSNDGFGNFFAHMTEVSSSKNETWNALKLAQESLPFENIKVSPASTTNPGIPP 448

Query: 422 SSLMAFLAKNPQISGVVLDDFDTGFTNQFYQSHLDDLHNINSSAIEAAALLVARTLYILA 481
           SSLMAFLAKN Q+SGVVL+DFDT FTNQFYQSHLDDLHNINSSAIEAAALLVARTLYILA
Sbjct: 449 SSLMAFLAKNSQVSGVVLEDFDTSFTNQFYQSHLDDLHNINSSAIEAAALLVARTLYILA 508

Query: 482 INKNELSSSVLTAIKVNTSLVEELIGCLLNCDPGLSCELVKRYITPSSVCPNHYVGVILD 541
            NK ELSSS L AIK+NTSLVEELIGCLLNCDPGLSCELVKRYI+P++VCPNHYVGVILD
Sbjct: 509 TNKKELSSSALNAIKLNTSLVEELIGCLLNCDPGLSCELVKRYISPTNVCPNHYVGVILD 568

Query: 542 EPSSAPYPDYVHDVSRFVWNFLADRTSIPKENTSSVCSQNCDDRSEVCIGAETGKGTCVI 601
           EPSS PYP YVHDVSRF WNFLADRTSI KENTSSVCSQNCDD+SEVCIGAETGKGTCV+
Sbjct: 569 EPSSTPYPGYVHDVSRFAWNFLADRTSIRKENTSSVCSQNCDDKSEVCIGAETGKGTCVV 628

Query: 602 STTRYIPAYSTRLKFESGYWSVLPPNSSDHLGAVDPVWTESNWNTIGLRIYTIQAAAYDR 661
           STTRY+PAYSTRL F+SG W+VLP N+SD +GAVDPVWTESNWNTIGLR+YT+QA AYDR
Sbjct: 629 STTRYVPAYSTRLTFDSGSWNVLPLNASDPMGAVDPVWTESNWNTIGLRMYTVQATAYDR 688

Query: 662 FVLLGGITTTILAYFAIVAVRSSIIKALKRD 693
           FVLLGGITTTIL+YFAIVAVR SI+KALK+D
Sbjct: 689 FVLLGGITTTILSYFAIVAVRGSIMKALKKD 709

BLAST of Cmc06g0163481 vs. ExPASy Swiss-Prot
Match: Q8GUM5 (Nicastrin OS=Arabidopsis thaliana OX=3702 GN=At3g52640/At3g52650 PE=2 SV=1)

HSP 1 Score: 828.2 bits (2138), Expect = 7.0e-239
Identity = 418/677 (61.74%), Postives = 515/677 (76.07%), Query Frame = 0

Query: 19  MSSQFLYLLLFLTSLRLSSSDE-QKMESVPDLQNSMYLAVDGYPCIRLLNLSGEIGCSNP 78
           +S  F  +LL +  L LS +DE   +ESVPDLQ  MY+AVDG+PC+RLLNLSGEIGCSNP
Sbjct: 9   LSIAFTLVLLSILPLHLSLADEITSIESVPDLQKLMYVAVDGFPCVRLLNLSGEIGCSNP 68

Query: 79  GREKVVLPMINFKDADEILEPSAVLVSMDAISSFFTRLQDDSHFANNVGGVLIEPGTGIQ 138
           G  KVV P+I  KD  ++++P  +LV+ D +  FFTR+  D  FA+ +GGVL+E G+  Q
Sbjct: 69  GINKVVAPIIKLKDVKDLVQPHTILVTADEMEDFFTRVSTDLSFASKIGGVLVESGSNFQ 128

Query: 139 NRTEGFSPAQKFPQAKFAPYKKNDYEWNPIGSGIMWNRYNFPVFLISESSISSIQEAASK 198
            + +GFSP ++FPQA+F+PY+  +Y+WN   S IMW  YNFPV+L+SES IS++ E  SK
Sbjct: 129 QKLKGFSPDKRFPQAQFSPYENVEYKWNSAASSIMWRNYNFPVYLLSESGISAVHEILSK 188

Query: 199 NVKSKKDYVSNVAEFDLVMQTTKAGTHSSMSCLKEETCLPLGGYSVWSSLPPINTSSSDQ 258
                  Y S+VAEF++VM+TTKAGTH+S +CL+E TCLPLGGYSVWSSLPPI+ SSS+ 
Sbjct: 189 KKMKHGTYTSDVAEFNMVMETTKAGTHNSEACLQEGTCLPLGGYSVWSSLPPISVSSSNN 248

Query: 259 SKPVILTVASMDSASFFRDKSIGADSPISGLIALLAAVDALSHVDGLDDLHKQLVFVVFT 318
            KPV+LTVASMD+ASFFRDKS GADSPISGL+ALL AVDALS VDG+ +L KQLVF+V T
Sbjct: 249 RKPVVLTVASMDTASFFRDKSFGADSPISGLVALLGAVDALSRVDGISNLKKQLVFLVLT 308

Query: 319 GESWGYLGSRRFLLELDLQSDAVSGLSNRLIDMEELVVVLRQVFEIGSVGKSSNHGSGNF 378
           GE+WGYLGSRRFL ELDL SDAV+GLSN  I+          V EIGSVGK  + G   F
Sbjct: 309 GETWGYLGSRRFLHELDLHSDAVAGLSNTSIE---------TVLEIGSVGKGLSGGINTF 368

Query: 379 FAHMTEVSSSKNETWNALKLARESLPLENIKVSPASTTNPGIPPSSLMAFLAKNPQISGV 438
           FAH T VSS  N T +ALK+A++SL  +NIK+  A T NPGIPPSSLMAF+ KNPQ S V
Sbjct: 369 FAHKTRVSSVTNMTLDALKIAQDSLASKNIKILSADTANPGIPPSSLMAFMRKNPQTSAV 428

Query: 439 VLDDFDTGFTNQFYQSHLDDLHNINSSAIEAAALLVARTLYILAINKNELSSSVLTAIKV 498
           VL+DFDT F N+FY SHLDDL NINSS++ AAA +VARTLYILA +  + S+S L +I V
Sbjct: 429 VLEDFDTNFVNKFYHSHLDDLSNINSSSVVAAASVVARTLYILASDNKDTSNSALGSIHV 488

Query: 499 NTSLVEELIGCLLNCDPGLSCELVKRYITPSSVCPNHYVGVILDEPSSAPYPDYVHDVSR 558
           N S VEEL+ CLL C+PGLSC LVK YI+P++ CP +Y GVIL EPSS PY  YV DVSR
Sbjct: 489 NASFVEELLTCLLACEPGLSCNLVKDYISPTNTCPGNYAGVILGEPSSKPYLGYVGDVSR 548

Query: 559 FVWNFLADRTSIPKENTSSVCSQN-CDDRSEVCIGAETGK-GTCVISTTRYIPAYSTRLK 618
           F+WNFLAD+TS+ K NT+SVCS+  C    EVCI AE+ K GTCV+STTRY+PAYSTRLK
Sbjct: 549 FLWNFLADKTSVQKGNTTSVCSKGVCSKTDEVCIKAESNKEGTCVVSTTRYVPAYSTRLK 608

Query: 619 FESGYWSVLPPNSSDHLGAVDPVWTESNWNTIGLRIYTIQAAAYDRFVLLGGITTTILAY 678
           +  G W++LP NSSD +G VDPVWTESNW+T+ + +YT+Q +AYD  VL+ GIT T LAY
Sbjct: 609 YNDGAWTILPQNSSDSMGMVDPVWTESNWDTLRVHVYTVQHSAYDNAVLVAGITVTTLAY 668

Query: 679 FAIVAVRSSIIKALKRD 693
             I+A +S I KALK+D
Sbjct: 669 IGILAAKSIITKALKQD 676

BLAST of Cmc06g0163481 vs. ExPASy Swiss-Prot
Match: Q54JT7 (Nicastrin OS=Dictyostelium discoideum OX=44689 GN=ncstn PE=3 SV=2)

HSP 1 Score: 189.1 bits (479), Expect = 1.7e-46
Identity = 170/685 (24.82%), Postives = 310/685 (45.26%), Query Frame = 0

Query: 43  MESVPDLQNSMYLAVDGYPCIRLLNLSGEIGCSNP-GREKVVLPMINFKDADEIL----- 102
           + S   +++ MY +++ YPC R++ L+G+IGCS+  G +  +L +I   D+DE       
Sbjct: 21  ISSQSSIEDKMYTSLNSYPCTRIMTLNGQIGCSSSHGGDSGILYLI---DSDESYHNYFS 80

Query: 103 --EPSAVLVSMDAISSFFTR-LQDDSHFANNVGGVLIEPGTGIQNRTEGFSPAQKFPQAK 162
             +   ++V  D  S++F + L  + +    + G L+    G   +T  +SP  ++P  +
Sbjct: 81  YNQQKDIIVVFD--SNYFNKTLVLEMYSKKKMNGALVLTDIG---KTYPYSPEDQYPIKQ 140

Query: 163 FAPYKKNDYEWNPIGSGIMWNRYNFPVFLISESSISSIQEAASKNVKSKKDYVSNVAEFD 222
           F  Y  ++  WNP G G  +  + FP+F +   +   I+  ++ N   K  Y +  AE D
Sbjct: 141 FGLYPDSNLNWNPNGDGFTYMNFPFPMFALELKTSIIIRNLSTINRDGK--YPAYGAELD 200

Query: 223 LVMQTTKAGTHSSMSCLKEETCLPLGGYSVWSSLPPINTSSSDQSKPVILTVASMDSASF 282
             MQ    G  ++ +CL+   C P+GG S+WSS   +     DQSKP+IL +  +D+ +F
Sbjct: 201 SFMQ----GAINAETCLRRGFCEPVGGQSIWSSFSEV----IDQSKPIILVMLPIDATAF 260

Query: 283 FRDKSIGADSPISGLIALLAAVDALSHVDGLDDLHKQLVFVVFTGESWGYLGSRRFLLEL 342
           FRD + G D     L  LL+ ++ L  VD      K+++F ++  E WGY+GS  F+ +L
Sbjct: 261 FRDLATGTDQSGYALTVLLSMLNTLQGVD-KTKWDKEVIFAMWNSERWGYVGSTNFVNDL 320

Query: 343 ------DLQSDAVSGLSN-RLIDMEELVVVLRQVF---EIGSVGKSSNHGSG-------- 402
                  L S+  +  S+  ++D+    +    ++   E   +G+  N G          
Sbjct: 321 LNFNCTSLDSNNQNSCSSPPMLDLTFEQIKFENIYAIIEFNQIGRPVNSGKKTPNKLDIY 380

Query: 403 NFFAHMTEVSSSKNETWNALKLARESLPLENIKVSPASTTNPGIPPSSLMAFLAKNPQ-- 462
           N   H     +  N+  +    + +S   EN  +    TT   +PP S M+F+ +  +  
Sbjct: 381 NLVFH-PNGGAGANQLMDVFSQSTQS--YENSTIQFQKTTQNELPPCSSMSFIKEINKKS 440

Query: 463 ----ISGVVLDDFDTGFTNQFYQSHLDDLHNIN--SSAIEAAALLVARTLYILAINKNEL 522
               I  +V+ D D  + N ++    D+  NIN  +S +     + ++++ +LA      
Sbjct: 441 APNFIGTLVITDHDYQYNNPYFGDEQDNSGNINTTTSTLFDMVQVFSKSIDLLAGGNG-- 500

Query: 523 SSSVLTAIKVNTSLVEELIGCLLNCDPGLSCELVKRYIT--PSSVCPNHYVGVILDEPSS 582
                  +KV+   + E+  CL      ++C  V + ++  P +  PN Y GV       
Sbjct: 501 ------TVKVDDLFIREINVCLTQ---SITCNWVTKLMSTFPYNPIPNFYSGVY----GV 560

Query: 583 APYPDYVHDVSRFVWNFLADRTSIPKENTSSVCSQNCDDRSEVCIGAETGKGTCVISTTR 642
           +P        +RF++      T      T+     +CD  S +C+        C+ S T 
Sbjct: 561 SPVNHITPIETRFIFRMATYLTQHRTNATNCTSDNDCDTSSSICVNK-----VCLYSNTH 620

Query: 643 YIPAYSTRLKFESGYWSVLPPNSSDHLGAVDPVWTESNWNTIGLRIYTIQAAAYDRFVLL 691
           Y  A S    F++   S    N+S       PV+ ESNW+   +R++ + + A + + L+
Sbjct: 621 YHNAISLAFSFDNSKSSWTIVNTS------YPVFVESNWDYTTVRLFQVGSYANEIWFLV 657

BLAST of Cmc06g0163481 vs. ExPASy Swiss-Prot
Match: F0ZBA6 (Nicastrin OS=Dictyostelium purpureum OX=5786 GN=ncstn PE=1 SV=1)

HSP 1 Score: 184.1 bits (466), Expect = 5.3e-45
Identity = 165/686 (24.05%), Postives = 306/686 (44.61%), Query Frame = 0

Query: 24  LYLLLFLTSLRLSSSDEQKMESVPDLQNSMYLAVDGYPCIRLLNLSGEIGCSNP-GREKV 83
           L LLL L    ++S          ++  ++Y     YPC +++   G+ GCS+  G    
Sbjct: 7   LVLLLLLVFSVINSEPSAPATISDNIYTTLY---SSYPCTKIMTSDGQFGCSSKHGGNSG 66

Query: 84  VLPMI----NFKDADEILEPSAVLVSMDAISSFFTRLQDDSHFANNVGGVLIEPGTGIQN 143
           +L +I    ++ +     +   ++V +D  + F +    + H  + + G+++   T    
Sbjct: 67  ILYLIDDDESYNNYFSYSQQKDIIVVLDT-NYFNSTSVLNLHNKSKIEGIIVLTDT---K 126

Query: 144 RTEGFSPAQKFPQAKFAPYKKNDYEWNPIGSGIMWNRYNFPVFLISESSISSIQEAASKN 203
           +T  +SP  ++P   +  Y  ++ EWNP   G  +  + FP+F I   +  +I+  +  N
Sbjct: 127 KTYPYSPDSRYPNKIYGLYPNSNLEWNPNADGFTYFSFPFPIFAIDNQTSVAIRNVSKHN 186

Query: 204 VKSKKDYVSNVAEFDLVMQTTKAGTHSSMSCLKEETCLPLGGYSVWSSLPPINTSSSDQS 263
              +  Y +  AE D  MQ    G  +S +CL+   C P+GG S+WSS     +S  D+ 
Sbjct: 187 RDGQ--YPAWGAELDSFMQ----GAINSETCLRRGFCEPVGGQSIWSSF----SSKIDKE 246

Query: 264 KPVILTVASMDSASFFRDKSIGADSPISGLIALLAAVDALSHVDGLDDLHKQLVFVVFTG 323
           K +IL +   D+ +FFRD SIGAD      + LL+ + +L+ VD     +K++VF  +  
Sbjct: 247 KEIILVMLPFDTTAFFRDLSIGADQSSFATVTLLSVIKSLAAVD-RSSWNKEVVFAFWNA 306

Query: 324 ESWGYLGSRRFLLE-LDLQSDAVSGLSNRLIDMEELVVV---------LRQVFEIGSVGK 383
           E WGY+GS  F+ + L+ Q    +   ++ ID     +          +  + E+  +G+
Sbjct: 307 ERWGYVGSEYFINDLLNFQCKTYNSDKSKCIDPPRADLAFQTQINFTKISTIIELNQIGR 366

Query: 384 SS-NHGSGNFFAHMTEVSSSKNETWNALKLARESLPLENIKVSPASTTNPGIPPSSLMAF 443
           +  +   G +  ++    +  +   + L     S   EN  ++   TT   +PPSS M+F
Sbjct: 367 AQLDKNLGKYSLYLHTAGTKTSSVTDILDQVASS--YENSTITFKPTTQTELPPSSSMSF 426

Query: 444 LAKNPQISGVVLDDFDTGFTNQFYQSHLDDLHNINSSAIEAAALLVARTLYILAINKNEL 503
           L K  +I  VV+ D D  ++N +Y    DD  N+  S +     +++  +  +A   N  
Sbjct: 427 LKKTNKIPVVVITDHDYKYSNPYYGYEQDDNENVLGSTLNDIVYILSTFIDRIAGGNNN- 486

Query: 504 SSSVLTAIKVNTSLVEELIGCLLNCDPGLSCELVKRYITPSSVCPNHYVGVILDEPSSAP 563
                  I ++ + +  L  C  +    ++C  +     P +  PN Y  V     ++  
Sbjct: 487 -------ITIDKNFINILYPCFTS---SITCFNILMKTYPLNEVPNFYSSVFGTSLTTTL 546

Query: 564 YPDYVHDVSRFVWNFLADRTSIPKEN-TSSVCSQNCDDRSEVCIGAETGKGTCVISTTRY 623
            P     + R ++       SI + N T + C+ + D  S +C       G CV S T  
Sbjct: 547 SPYETKLIHRLLY-------SITQYNSTLTNCTSDNDCPSSLCY-----SGQCVSSNTHL 606

Query: 624 IPAYSTRLKFES--GYWSVLPPNSSDHLGAVDPVWTESNWNTIGLRIYTIQAAAYDRFVL 683
             A S    F++    W ++  NSS       P++TESNW+   L+++ I  +  + + L
Sbjct: 607 HNALSLGFDFDTSKNVWKIV--NSS------YPIFTESNWDYTALKVFKIGNSTTEIWFL 641

Query: 684 LGGITTTILAYFAIVAVRSSIIKALK 691
           + G+   +++   I+ V+  +    K
Sbjct: 667 VSGLIELLVSIGLILYVKKFLSNRYK 641

BLAST of Cmc06g0163481 vs. ExPASy Swiss-Prot
Match: P57716 (Nicastrin OS=Mus musculus OX=10090 GN=Ncstn PE=1 SV=3)

HSP 1 Score: 137.1 bits (344), Expect = 7.5e-31
Identity = 183/700 (26.14%), Postives = 296/700 (42.29%), Query Frame = 0

Query: 61  PCIRLLNLSGEIGC--SNPGREKVVLPMINFKDADEIL-----EPSAVLVSMDAISSFFT 120
           PC+RLLN + +IGC  S  G   V+  +   +D   +L      P  VL+        FT
Sbjct: 48  PCVRLLNATHQIGCQSSISGDTGVIHVVEKEEDLKWVLTDGPNPPYMVLLE----GKLFT 107

Query: 121 R--LQDDSHFANNVGGVLIEPGTGIQNRTEGFSPAQKFPQAKFAPYKKNDYE-------- 180
           R  ++      + + G+ +       N T  FSP+ + P   F  Y  N Y         
Sbjct: 108 RDVMEKLKGTTSRIAGLAVT--LAKPNSTSSFSPSVQCPNDGFGIY-SNSYGPEFAHCKK 167

Query: 181 --WNPIGSGIMWNRYNFPVFLISESSISSIQEAASKNVKSKKDYVSNVAEFDLVMQTTKA 240
             WN +G+G+ +  ++FP+FL+ + + + + +   ++    ++   +   F L      +
Sbjct: 168 TLWNELGNGLAYEDFSFPIFLLEDENETKVIKQCYQDHNLGQN--GSAPSFPLCAMQLFS 227

Query: 241 GTH---SSMSCLK------------EETCLPLGGYSVWSSLPPINTS-SSDQSKPVILTV 300
             H   S+ +C++            E  C PL  Y+VWS L PINTS   +    V++  
Sbjct: 228 HMHAVISTATCMRRSFIQSTFSINPEIVCDPLSDYNVWSMLKPINTSVGLEPDVRVVVAA 287

Query: 301 ASMDSASFFRDKSIGADSPISGLIALLAAVDALSHVDGLDDLHKQLVFVVFTGESWGYLG 360
             +DS SFF + + GA+S ++  +  LAA +AL     +  L + ++FV F GE++ Y+G
Sbjct: 288 TRLDSRSFFWNVAPGAESAVASFVTQLAAAEALHKAPDVTTLSRNVMFVFFQGETFDYIG 347

Query: 361 SRRFLLELDLQSDAVSGLSNRLIDMEELVVVLRQVFEIGSVGKSSNHGSGNFFAHMTEVS 420
           S R + +++     V     RL +++  V       E+G V   +   S + + H T+  
Sbjct: 348 SSRMVYDMENGKFPV-----RLENIDSFV-------ELGQVALRT---SLDLWMH-TDPM 407

Query: 421 SSKNETWNALKLARESLPLENIKVSPAS---------TTNPGIPPSSLMAFL-AKNPQIS 480
           S KNE   ++K   E L L  ++ S A            +  +PPSSL  FL A+N  IS
Sbjct: 408 SQKNE---SVKNQVEDL-LATLEKSGAGVPEVVLRRLAQSQALPPSSLQRFLRARN--IS 467

Query: 481 GVVLDDFDTGFTNQFYQSHLDDLHNIN-------------------SSAIEAAALLVART 540
           GVVL D    F N++YQS  D   NIN                   + A+   A ++AR 
Sbjct: 468 GVVLADHSGSFHNRYYQSIYDTAENINVTYPEWQSPEEDLNFVTDTAKALANVATVLARA 527

Query: 541 LYILAINKNELSSSVLTAIKVNTSLVEELIGCLLNCDPGLSCELVKR----YITPSSVCP 600
           LY LA   N  SSS+    +  T L   L G L+  +      ++K     Y+    +  
Sbjct: 528 LYELAGGTN-FSSSIQADPQTVTRL---LYGFLVRANNSWFQSILKHDLRSYLDDRPL-- 587

Query: 601 NHYVGVILDEPSSAPYPDYVHDVSRFVWNFLADRTSIPKE---NTSSVCSQNCDDRSEVC 660
            HY+ V     SS     YV  V   + N     T++ +E   + S V +++ D      
Sbjct: 588 QHYIAV-----SSPTNTTYV--VQYALANLTGKATNLTREQCQDPSKVPNESKDLYEYSW 647

Query: 661 IGAETGKG------TCVISTTRYIPAYSTRLKFESGYWSVLPPNSSDHLGAVDPVWTESN 684
           +              CV ST R   A S    FE   WS     S+++       W ES 
Sbjct: 648 VQGPWNSNRTERLPQCVRSTVRLARALSP--AFELSQWS-----STEY-----STWAESR 691

BLAST of Cmc06g0163481 vs. ExPASy Swiss-Prot
Match: Q92542 (Nicastrin OS=Homo sapiens OX=9606 GN=NCSTN PE=1 SV=2)

HSP 1 Score: 135.6 bits (340), Expect = 2.2e-30
Identity = 170/689 (24.67%), Postives = 282/689 (40.93%), Query Frame = 0

Query: 61  PCIRLLNLSGEIGC--SNPGREKVVLPMINFKDADEIL-----EPSAVLVSMDAISSFFT 120
           PC+RLLN + +IGC  S  G   V+  +   +D   +L      P  VL+     S  FT
Sbjct: 49  PCVRLLNATHQIGCQSSISGDTGVIHVVEKEEDLQWVLTDGPNPPYMVLLE----SKHFT 108

Query: 121 R--LQDDSHFANNVGGVLIEPGTGIQNRTEGFSPAQKFPQAKFAPYKKN---------DY 180
           R  ++      + + G+ +       +   GFSP+ + P   F  Y  +         + 
Sbjct: 109 RDLMEKLKGRTSRIAGLAV--SLTKPSPASGFSPSVQCPNDGFGVYSNSYGPEFAHCREI 168

Query: 181 EWNPIGSGIMWNRYNFPVFLISESSISSIQEAASKNVKSKKD---------YVSNVAEFD 240
           +WN +G+G+ +  ++FP+FL+ + + + + +   ++    ++          +   +   
Sbjct: 169 QWNSLGNGLAYEDFSFPIFLLEDENETKVIKQCYQDHNLSQNGSAPTFPLCAMQLFSHMH 228

Query: 241 LVMQTT----KAGTHSSMSCLKEETCLPLGGYSVWSSLPPINTSSS-DQSKPVILTVASM 300
            V+ T     ++   S+ S   E  C PL  Y+VWS L PINT+ +      V++    +
Sbjct: 229 AVISTATCMRRSSIQSTFSINPEIVCDPLSDYNVWSMLKPINTTGTLKPDDRVVVAATRL 288

Query: 301 DSASFFRDKSIGADSPISGLIALLAAVDALSHVDGLDDLHKQLVFVVFTGESWGYLGSRR 360
           DS SFF + + GA+S ++  +  LAA +AL     +  L + ++FV F GE++ Y+GS R
Sbjct: 289 DSRSFFWNVAPGAESAVASFVTQLAAAEALQKAPDVTTLPRNVMFVFFQGETFDYIGSSR 348

Query: 361 FLLELDLQSDAVSGLSNRLIDMEELVVVLRQVFEIGSVGKSSNHGSGNFFAHMTEVSSSK 420
            + ++               +  +  V L  V     +G+ +   S   + H   VS   
Sbjct: 349 MVYDM---------------EKGKFPVQLENVDSFVELGQVALRTSLELWMHTDPVSQKN 408

Query: 421 NETWNALKLARESLPLENIKVSPASTTNPG----IPPSSLMAFL-AKNPQISGVVLDDFD 480
               N ++    +L      V       P     +PPSSL  FL A+N  ISGVVL D  
Sbjct: 409 ESVRNQVEDLLATLEKSGAGVPAVILRRPNQSQPLPPSSLQRFLRARN--ISGVVLADHS 468

Query: 481 TGFTNQFYQSHLDDLHNINSS-------------------AIEAAALLVARTLYILAINK 540
             F N++YQS  D   NIN S                   A+   A ++ R LY LA   
Sbjct: 469 GAFHNKYYQSIYDTAENINVSYPEWLSPEEDLNFVTDTAKALADVATVLGRALYELAGGT 528

Query: 541 NELSSSVLTAIKVNTSLVEELIGCLLNCDPGLSCELVKR----YITPSSVCPNHYVGVIL 600
           N  S +V    +  T L   L G L+  +      ++++    Y+    +   HY+ V  
Sbjct: 529 N-FSDTVQADPQTVTRL---LYGFLIKANNSWFQSILRQDLRSYLGDGPL--QHYIAV-- 588

Query: 601 DEPSSAPYPDYVHDVSRFVWNFLADRTSIPKENTSSVCSQNCDDRSEVCI-----GAETG 660
             P++  Y   V      +   + + T    ++ S V S+N D      +       ET 
Sbjct: 589 SSPTNTTY--VVQYALANLTGTVVNLTREQCQDPSKVPSENKDLYEYSWVQGPLHSNETD 648

Query: 661 K-GTCVISTTRYIPAYSTRLKFESGYWSVLPPNSSDHLGAVDPVWTESNWNTIGLRIYTI 684
           +   CV ST R   A S    FE   WS     S+++       WTES W  I  RI+ I
Sbjct: 649 RLPRCVRSTARLARALSP--AFELSQWS-----STEY-----STWTESRWKDIRARIFLI 692

BLAST of Cmc06g0163481 vs. ExPASy TrEMBL
Match: A0A1S3C4C7 (Nicastrin OS=Cucumis melo OX=3656 GN=LOC103496866 PE=3 SV=1)

HSP 1 Score: 1338.6 bits (3463), Expect = 0.0e+00
Identity = 683/692 (98.70%), Postives = 683/692 (98.70%), Query Frame = 0

Query: 1   MNDPTDSDLIERTPKSSSMSSQFLYLLLFLTSLRLSSSDEQKMESVPDLQNSMYLAVDGY 60
           MNDPTDSDLIERTPKSSSMSSQFLYLLLFLTSLRLSSSDEQKMESVPDLQNSMYLAVDGY
Sbjct: 1   MNDPTDSDLIERTPKSSSMSSQFLYLLLFLTSLRLSSSDEQKMESVPDLQNSMYLAVDGY 60

Query: 61  PCIRLLNLSGEIGCSNPGREKVVLPMINFKDADEILEPSAVLVSMDAISSFFTRLQDDSH 120
           PCIRLLNLSGEIGCSNPGREKVVLPMINFKDADEILEPSAVLVSMDAISSFFTRLQDDSH
Sbjct: 61  PCIRLLNLSGEIGCSNPGREKVVLPMINFKDADEILEPSAVLVSMDAISSFFTRLQDDSH 120

Query: 121 FANNVGGVLIEPGTGIQNRTEGFSPAQKFPQAKFAPYKKNDYEWNPIGSGIMWNRYNFPV 180
           FANNVGGVLIEPGTGIQNRTEGFSPAQKFPQAKFAPYKKNDYEWNPIGSGIMWNRYNFPV
Sbjct: 121 FANNVGGVLIEPGTGIQNRTEGFSPAQKFPQAKFAPYKKNDYEWNPIGSGIMWNRYNFPV 180

Query: 181 FLISESSISSIQEAASKNVKSKKDYVSNVAEFDLVMQTTKAGTHSSMSCLKEETCLPLGG 240
           FLISESSISSIQEAASKNVKSKKDYVSNVAEFDLVMQTTKAGTHSSMSCLKEETCLPLGG
Sbjct: 181 FLISESSISSIQEAASKNVKSKKDYVSNVAEFDLVMQTTKAGTHSSMSCLKEETCLPLGG 240

Query: 241 YSVWSSLPPINTSSSDQSKPVILTVASMDSASFFRDKSIGADSPISGLIALLAAVDALSH 300
           YSVWSSLPPINTSSSDQSKPVILTVASMDSASFFRDKSIGADSPISGLIALLAAVDALSH
Sbjct: 241 YSVWSSLPPINTSSSDQSKPVILTVASMDSASFFRDKSIGADSPISGLIALLAAVDALSH 300

Query: 301 VDGLDDLHKQLVFVVFTGESWGYLGSRRFLLELDLQSDAVSGLSNRLIDMEELVVVLRQV 360
           VDGLDDLHKQLVFVVFTGESWGYLGSRRFLLELDLQSDAVSGLSNRLIDM         V
Sbjct: 301 VDGLDDLHKQLVFVVFTGESWGYLGSRRFLLELDLQSDAVSGLSNRLIDM---------V 360

Query: 361 FEIGSVGKSSNHGSGNFFAHMTEVSSSKNETWNALKLARESLPLENIKVSPASTTNPGIP 420
           FEIGSVGKSSNHGSGNFFAHMTEVSSSKNETWNALKLARESLPLENIKVSPASTTNPGIP
Sbjct: 361 FEIGSVGKSSNHGSGNFFAHMTEVSSSKNETWNALKLARESLPLENIKVSPASTTNPGIP 420

Query: 421 PSSLMAFLAKNPQISGVVLDDFDTGFTNQFYQSHLDDLHNINSSAIEAAALLVARTLYIL 480
           PSSLMAFLAKNPQISGVVLDDFDTGFTNQFYQSHLDDLHNINSSAIEAAALLVARTLYIL
Sbjct: 421 PSSLMAFLAKNPQISGVVLDDFDTGFTNQFYQSHLDDLHNINSSAIEAAALLVARTLYIL 480

Query: 481 AINKNELSSSVLTAIKVNTSLVEELIGCLLNCDPGLSCELVKRYITPSSVCPNHYVGVIL 540
           AINKNELSSSVLTAIKVNTSLVEELIGCLLNCDPGLSCELVKRYITPSSVCPNHYVGVIL
Sbjct: 481 AINKNELSSSVLTAIKVNTSLVEELIGCLLNCDPGLSCELVKRYITPSSVCPNHYVGVIL 540

Query: 541 DEPSSAPYPDYVHDVSRFVWNFLADRTSIPKENTSSVCSQNCDDRSEVCIGAETGKGTCV 600
           DEPSSAPYPDYVHDVSRFVWNFLADRTSIPKENTSSVCSQNCDDRSEVCIGAETGKGTCV
Sbjct: 541 DEPSSAPYPDYVHDVSRFVWNFLADRTSIPKENTSSVCSQNCDDRSEVCIGAETGKGTCV 600

Query: 601 ISTTRYIPAYSTRLKFESGYWSVLPPNSSDHLGAVDPVWTESNWNTIGLRIYTIQAAAYD 660
           ISTTRYIPAYSTRLKFESGYWSVLPPNSSDHLGAVDPVWTESNWNTIGLRIYTIQAAAYD
Sbjct: 601 ISTTRYIPAYSTRLKFESGYWSVLPPNSSDHLGAVDPVWTESNWNTIGLRIYTIQAAAYD 660

Query: 661 RFVLLGGITTTILAYFAIVAVRSSIIKALKRD 693
           RFVLLGGITTTILAYFAIVAVRSSIIKALKRD
Sbjct: 661 RFVLLGGITTTILAYFAIVAVRSSIIKALKRD 683

BLAST of Cmc06g0163481 vs. ExPASy TrEMBL
Match: A0A1S3C4S1 (Nicastrin OS=Cucumis melo OX=3656 GN=LOC103496866 PE=3 SV=1)

HSP 1 Score: 1281.5 bits (3315), Expect = 0.0e+00
Identity = 659/692 (95.23%), Postives = 659/692 (95.23%), Query Frame = 0

Query: 1   MNDPTDSDLIERTPKSSSMSSQFLYLLLFLTSLRLSSSDEQKMESVPDLQNSMYLAVDGY 60
           MNDPTDSDLIERTPKSSSMSSQFLYLLLFLTSLRLSSSDEQKMESVPDLQNSMYLAVDGY
Sbjct: 1   MNDPTDSDLIERTPKSSSMSSQFLYLLLFLTSLRLSSSDEQKMESVPDLQNSMYLAVDGY 60

Query: 61  PCIRLLNLSGEIGCSNPGREKVVLPMINFKDADEILEPSAVLVSMDAISSFFTRLQDDSH 120
           PCIRLLNLSGEIGCSNPGREKVVLPMINFKDADEILEPSAVLVSMDAISSFFTRLQDDSH
Sbjct: 61  PCIRLLNLSGEIGCSNPGREKVVLPMINFKDADEILEPSAVLVSMDAISSFFTRLQDDSH 120

Query: 121 FANNVGGVLIEPGTGIQNRTEGFSPAQKFPQAKFAPYKKNDYEWNPIGSGIMWNRYNFPV 180
           FANNVGGVLIEPGTGIQNRTEGFSPAQKFPQAKFAPYKKNDYEWNPIGSGIMWNRYNFPV
Sbjct: 121 FANNVGGVLIEPGTGIQNRTEGFSPAQKFPQAKFAPYKKNDYEWNPIGSGIMWNRYNFPV 180

Query: 181 FLISESSISSIQEAASKNVKSKKDYVSNVAEFDLVMQTTKAGTHSSMSCLKEETCLPLGG 240
           FLISESSISSIQEAASKNVKSKKDYVSNVAEFDLVMQTTKAGTHSSMSCLKEETCLPLGG
Sbjct: 181 FLISESSISSIQEAASKNVKSKKDYVSNVAEFDLVMQTTKAGTHSSMSCLKEETCLPLGG 240

Query: 241 YSVWSSLPPINTSSSDQSKPVILTVASMDSASFFRDKSIGADSPISGLIALLAAVDALSH 300
           YSVWSSLPPINTSSSDQSKPVILTVASMDSASFFRDKSIGADSPISGLIALLAAVDALSH
Sbjct: 241 YSVWSSLPPINTSSSDQSKPVILTVASMDSASFFRDKSIGADSPISGLIALLAAVDALSH 300

Query: 301 VDGLDDLHKQLVFVVFTGESWGYLGSRRFLLELDLQSDAVSGLSNRLIDMEELVVVLRQV 360
           VDGLDDLHKQLVFVVFTGESWGYLGSRRFLLELDLQSDAVSGLSNRLIDM          
Sbjct: 301 VDGLDDLHKQLVFVVFTGESWGYLGSRRFLLELDLQSDAVSGLSNRLIDM---------- 360

Query: 361 FEIGSVGKSSNHGSGNFFAHMTEVSSSKNETWNALKLARESLPLENIKVSPASTTNPGIP 420
                                  VSSSKNETWNALKLARESLPLENIKVSPASTTNPGIP
Sbjct: 361 -----------------------VSSSKNETWNALKLARESLPLENIKVSPASTTNPGIP 420

Query: 421 PSSLMAFLAKNPQISGVVLDDFDTGFTNQFYQSHLDDLHNINSSAIEAAALLVARTLYIL 480
           PSSLMAFLAKNPQISGVVLDDFDTGFTNQFYQSHLDDLHNINSSAIEAAALLVARTLYIL
Sbjct: 421 PSSLMAFLAKNPQISGVVLDDFDTGFTNQFYQSHLDDLHNINSSAIEAAALLVARTLYIL 480

Query: 481 AINKNELSSSVLTAIKVNTSLVEELIGCLLNCDPGLSCELVKRYITPSSVCPNHYVGVIL 540
           AINKNELSSSVLTAIKVNTSLVEELIGCLLNCDPGLSCELVKRYITPSSVCPNHYVGVIL
Sbjct: 481 AINKNELSSSVLTAIKVNTSLVEELIGCLLNCDPGLSCELVKRYITPSSVCPNHYVGVIL 540

Query: 541 DEPSSAPYPDYVHDVSRFVWNFLADRTSIPKENTSSVCSQNCDDRSEVCIGAETGKGTCV 600
           DEPSSAPYPDYVHDVSRFVWNFLADRTSIPKENTSSVCSQNCDDRSEVCIGAETGKGTCV
Sbjct: 541 DEPSSAPYPDYVHDVSRFVWNFLADRTSIPKENTSSVCSQNCDDRSEVCIGAETGKGTCV 600

Query: 601 ISTTRYIPAYSTRLKFESGYWSVLPPNSSDHLGAVDPVWTESNWNTIGLRIYTIQAAAYD 660
           ISTTRYIPAYSTRLKFESGYWSVLPPNSSDHLGAVDPVWTESNWNTIGLRIYTIQAAAYD
Sbjct: 601 ISTTRYIPAYSTRLKFESGYWSVLPPNSSDHLGAVDPVWTESNWNTIGLRIYTIQAAAYD 659

Query: 661 RFVLLGGITTTILAYFAIVAVRSSIIKALKRD 693
           RFVLLGGITTTILAYFAIVAVRSSIIKALKRD
Sbjct: 661 RFVLLGGITTTILAYFAIVAVRSSIIKALKRD 659

BLAST of Cmc06g0163481 vs. ExPASy TrEMBL
Match: A0A6J1K5Z2 (Nicastrin OS=Cucurbita maxima OX=3661 GN=LOC111492619 PE=3 SV=1)

HSP 1 Score: 1166.8 bits (3017), Expect = 0.0e+00
Identity = 588/674 (87.24%), Postives = 622/674 (92.28%), Query Frame = 0

Query: 19  MSSQFLYLLLFLTSLRLSSSDEQKMESVPDLQNSMYLAVDGYPCIRLLNLSGEIGCSNPG 78
           M+   L+LLLFLTS RL  SDE  MESVPDLQNSMYL VDGYPCIRLLNLSGEIGCSNPG
Sbjct: 1   MAFHILHLLLFLTSARLCLSDEHSMESVPDLQNSMYLVVDGYPCIRLLNLSGEIGCSNPG 60

Query: 79  REKVVLPMINFKDADEILEPSAVLVSMDAISSFFTRLQDDSHFANNVGGVLIEPGTGIQN 138
           REKVV+PMINFKDADEI +PSA+LVSMD ISSFF RLQDDS+FA+NVGGVLI+PGT IQ 
Sbjct: 61  REKVVVPMINFKDADEIFQPSAILVSMDDISSFFARLQDDSNFASNVGGVLIKPGTEIQK 120

Query: 139 RTEGFSPAQKFPQAKFAPYKKNDYEWNPIGSGIMWNRYNFPVFLISESSISSIQEAASKN 198
           RT+GFSPAQKFPQAKFAPY+K DYEWNPIGSG+MWN+YNFPVFLISESSIS +QEAASKN
Sbjct: 121 RTKGFSPAQKFPQAKFAPYQKIDYEWNPIGSGVMWNQYNFPVFLISESSISPVQEAASKN 180

Query: 199 VKSKKDYVSNVAEFDLVMQTTKAGTHSSMSCLKEETCLPLGGYSVWSSLPPINTSSSDQS 258
           VK KK Y SNVAEFDLVMQTTKAGTH+SMSCLKEETCLPLGGYSVWSSLPPIN  S D+S
Sbjct: 181 VKDKKVYTSNVAEFDLVMQTTKAGTHNSMSCLKEETCLPLGGYSVWSSLPPINI-SLDKS 240

Query: 259 KPVILTVASMDSASFFRDKSIGADSPISGLIALLAAVDALSHVDGLDDLHKQLVFVVFTG 318
           KP+ILTVASMDSASFFRDKSIGADSPISGLIALLAAVDALSHVDGLDDLHKQLVFVVFTG
Sbjct: 241 KPIILTVASMDSASFFRDKSIGADSPISGLIALLAAVDALSHVDGLDDLHKQLVFVVFTG 300

Query: 319 ESWGYLGSRRFLLELDLQSDAVSGLSNRLIDMEELVVVLRQVFEIGSVGKSSNHGSGNFF 378
           ESWGYLGSRRFLLELDLQSD+VSGL+N LID          VFEIGSVGK SN G GNFF
Sbjct: 301 ESWGYLGSRRFLLELDLQSDSVSGLNNTLID---------TVFEIGSVGKKSNDGFGNFF 360

Query: 379 AHMTEVSSSKNETWNALKLARESLPLENIKVSPASTTNPGIPPSSLMAFLAKNPQISGVV 438
           AHMTEVSSSKNETWNALKLA+ESLP ENIKVSPASTTNPGIPPSSLMAFLAKN Q+SGVV
Sbjct: 361 AHMTEVSSSKNETWNALKLAQESLPFENIKVSPASTTNPGIPPSSLMAFLAKNSQVSGVV 420

Query: 439 LDDFDTGFTNQFYQSHLDDLHNINSSAIEAAALLVARTLYILAINKNELSSSVLTAIKVN 498
           L+DFDT FTNQFYQSHLDDLHNINSSAIEAAALLVARTLYILA NK ELSSS L AIK+N
Sbjct: 421 LEDFDTSFTNQFYQSHLDDLHNINSSAIEAAALLVARTLYILATNKKELSSSALNAIKLN 480

Query: 499 TSLVEELIGCLLNCDPGLSCELVKRYITPSSVCPNHYVGVILDEPSSAPYPDYVHDVSRF 558
           TSLVEE+IGCLLNCDPGLSCELVKRYI+P +VCPNHYVGVILDEPSS PYP YVHDVSRF
Sbjct: 481 TSLVEEIIGCLLNCDPGLSCELVKRYISPINVCPNHYVGVILDEPSSTPYPGYVHDVSRF 540

Query: 559 VWNFLADRTSIPKENTSSVCSQNCDDRSEVCIGAETGKGTCVISTTRYIPAYSTRLKFES 618
           VWNFLADRTSIPKENTSSVCSQNCDD+SEVCIGAETGKGTCV+STTRY+PAYSTRL FES
Sbjct: 541 VWNFLADRTSIPKENTSSVCSQNCDDKSEVCIGAETGKGTCVVSTTRYVPAYSTRLMFES 600

Query: 619 GYWSVLPPNSSDHLGAVDPVWTESNWNTIGLRIYTIQAAAYDRFVLLGGITTTILAYFAI 678
           G W+VLPPNSSD +GAVDPVWTESNWNTIGLR YT+QA AYDRFVLLGGITTTIL+YFAI
Sbjct: 601 GSWNVLPPNSSDPMGAVDPVWTESNWNTIGLRTYTVQATAYDRFVLLGGITTTILSYFAI 660

Query: 679 VAVRSSIIKALKRD 693
           VAVR SI+KALK+D
Sbjct: 661 VAVRGSIMKALKKD 664

BLAST of Cmc06g0163481 vs. ExPASy TrEMBL
Match: A0A6J1GAI9 (Nicastrin OS=Cucurbita moschata OX=3662 GN=LOC111452408 PE=3 SV=1)

HSP 1 Score: 1161.7 bits (3004), Expect = 0.0e+00
Identity = 585/674 (86.80%), Postives = 624/674 (92.58%), Query Frame = 0

Query: 19  MSSQFLYLLLFLTSLRLSSSDEQKMESVPDLQNSMYLAVDGYPCIRLLNLSGEIGCSNPG 78
           MS   L+LLLFLTS+RL  SDE  MESVPDLQNSMYL VDG+PCIRLLNLSGEIGCSNPG
Sbjct: 1   MSFHILHLLLFLTSVRLCLSDEHSMESVPDLQNSMYLVVDGHPCIRLLNLSGEIGCSNPG 60

Query: 79  REKVVLPMINFKDADEILEPSAVLVSMDAISSFFTRLQDDSHFANNVGGVLIEPGTGIQN 138
           REKVV+PMINFKDADEIL+PSA+LVSMD ISSFF RLQDDS+FA+NVGGVLI+PGT IQ 
Sbjct: 61  REKVVVPMINFKDADEILQPSAILVSMDDISSFFARLQDDSNFASNVGGVLIKPGTEIQK 120

Query: 139 RTEGFSPAQKFPQAKFAPYKKNDYEWNPIGSGIMWNRYNFPVFLISESSISSIQEAASKN 198
           RT+GFSPAQKFPQAKFAPY+K DYEWNPIGSG+MWN+YNFPVFLISESSIS +QEAASKN
Sbjct: 121 RTKGFSPAQKFPQAKFAPYQKIDYEWNPIGSGVMWNQYNFPVFLISESSISPVQEAASKN 180

Query: 199 VKSKKDYVSNVAEFDLVMQTTKAGTHSSMSCLKEETCLPLGGYSVWSSLPPINTSSSDQS 258
           VK KK Y SNVAEFDLVMQTTKAGT +SMSCLKEETCLPLGGYSVWSSLPPIN  SSD+S
Sbjct: 181 VKDKKIYTSNVAEFDLVMQTTKAGTRNSMSCLKEETCLPLGGYSVWSSLPPINI-SSDKS 240

Query: 259 KPVILTVASMDSASFFRDKSIGADSPISGLIALLAAVDALSHVDGLDDLHKQLVFVVFTG 318
           KP+ILTVASMDSASFFRDKSIGADSPISGLIALLA+VDALSHVDGLDDLHKQLVFVVFTG
Sbjct: 241 KPIILTVASMDSASFFRDKSIGADSPISGLIALLASVDALSHVDGLDDLHKQLVFVVFTG 300

Query: 319 ESWGYLGSRRFLLELDLQSDAVSGLSNRLIDMEELVVVLRQVFEIGSVGKSSNHGSGNFF 378
           ESWGYLGSRRFLLELDLQSD+VSGL+N LID          VFEIGSVGK SN G GNFF
Sbjct: 301 ESWGYLGSRRFLLELDLQSDSVSGLNNTLID---------TVFEIGSVGKKSNDGFGNFF 360

Query: 379 AHMTEVSSSKNETWNALKLARESLPLENIKVSPASTTNPGIPPSSLMAFLAKNPQISGVV 438
           AHMTEVSSSKNETWNALKLA+ESLP ENIKVSPASTTNPGIPPSSLMAFLAKN Q+SGVV
Sbjct: 361 AHMTEVSSSKNETWNALKLAQESLPFENIKVSPASTTNPGIPPSSLMAFLAKNSQVSGVV 420

Query: 439 LDDFDTGFTNQFYQSHLDDLHNINSSAIEAAALLVARTLYILAINKNELSSSVLTAIKVN 498
           L+DFDT FTNQFYQSHLDDLHNINSSAIEAAALLVARTLYILA NK ELSSS L AIK+N
Sbjct: 421 LEDFDTSFTNQFYQSHLDDLHNINSSAIEAAALLVARTLYILATNKKELSSSALNAIKLN 480

Query: 499 TSLVEELIGCLLNCDPGLSCELVKRYITPSSVCPNHYVGVILDEPSSAPYPDYVHDVSRF 558
           TSLVEELIGCLLNCDPGLSCELVKRYI+P++VCPNHYVGVILDEPSS PYP YVHDVSRF
Sbjct: 481 TSLVEELIGCLLNCDPGLSCELVKRYISPTNVCPNHYVGVILDEPSSTPYPGYVHDVSRF 540

Query: 559 VWNFLADRTSIPKENTSSVCSQNCDDRSEVCIGAETGKGTCVISTTRYIPAYSTRLKFES 618
            WNFLADRTSI KENTSSVCSQNCDD+SEVCIGAETGKGTCV+STTRY+PAYSTRL F+S
Sbjct: 541 AWNFLADRTSIRKENTSSVCSQNCDDKSEVCIGAETGKGTCVVSTTRYVPAYSTRLTFDS 600

Query: 619 GYWSVLPPNSSDHLGAVDPVWTESNWNTIGLRIYTIQAAAYDRFVLLGGITTTILAYFAI 678
           G W+VLPPN+SD +GAVDPVWTESNWNTIGLR+YT+QA AYDRFVLLGGITTTIL+YFAI
Sbjct: 601 GSWNVLPPNASDPMGAVDPVWTESNWNTIGLRMYTVQATAYDRFVLLGGITTTILSYFAI 660

Query: 679 VAVRSSIIKALKRD 693
           VAVR SI+KALK+D
Sbjct: 661 VAVRGSIMKALKKD 664

BLAST of Cmc06g0163481 vs. ExPASy TrEMBL
Match: A0A6J1CNM4 (Nicastrin OS=Momordica charantia OX=3673 GN=LOC111013258 PE=3 SV=1)

HSP 1 Score: 1147.9 bits (2968), Expect = 0.0e+00
Identity = 581/675 (86.07%), Postives = 621/675 (92.00%), Query Frame = 0

Query: 19  MSSQFLYLLLFLTSLRLSSSDE-QKMESVPDLQNSMYLAVDGYPCIRLLNLSGEIGCSNP 78
           MSS  LYLL+FLTSLRLS SD+   MESVPDLQNSMYL VDGYPCIRLLNLSGEIGCSNP
Sbjct: 1   MSSNILYLLVFLTSLRLSLSDDTHSMESVPDLQNSMYLVVDGYPCIRLLNLSGEIGCSNP 60

Query: 79  GREKVVLPMINFKDADEILEPSAVLVSMDAISSFFTRLQDDSHFANNVGGVLIEPGTGIQ 138
           GREKVV+PMINFKDADE+ +PSA++VSMD ISSFF+RL+DDS+FANNVGGVLIEPGT +Q
Sbjct: 61  GREKVVVPMINFKDADELSQPSAIVVSMDEISSFFSRLKDDSNFANNVGGVLIEPGTEMQ 120

Query: 139 NRTEGFSPAQKFPQAKFAPYKKNDYEWNPIGSGIMWNRYNFPVFLISESSISSIQEAASK 198
           NRTEGFSPA+KFPQA+FAPY+K DY+WNPIGSGIMW +YNFPVFLISE+SISSI EA+SK
Sbjct: 121 NRTEGFSPARKFPQAEFAPYQKVDYDWNPIGSGIMWKQYNFPVFLISETSISSIHEASSK 180

Query: 199 NVKSKKDYVSNVAEFDLVMQTTKAGTHSSMSCLKEETCLPLGGYSVWSSLPPINTSSSDQ 258
           NVK+KK Y SNVAEFDLVMQTTKAGTH+S+SCLKEETCLPLGGYSVWSSLPPIN  SSDQ
Sbjct: 181 NVKNKKAYTSNVAEFDLVMQTTKAGTHNSVSCLKEETCLPLGGYSVWSSLPPINI-SSDQ 240

Query: 259 SKPVILTVASMDSASFFRDKSIGADSPISGLIALLAAVDALSHVDGLDDLHKQLVFVVFT 318
           SKP+ILTVASMDSASFFRDKSIGADSPISGLIALLAAVDALSHVDGLDDLHKQLVF VFT
Sbjct: 241 SKPIILTVASMDSASFFRDKSIGADSPISGLIALLAAVDALSHVDGLDDLHKQLVFAVFT 300

Query: 319 GESWGYLGSRRFLLELDLQSDAVSGLSNRLIDMEELVVVLRQVFEIGSVGKSSNHGSGNF 378
           GESWGYLGSRRFLLELDLQSD VSGL+N+LID          VFEIGSVGKSS+HG GNF
Sbjct: 301 GESWGYLGSRRFLLELDLQSDVVSGLNNKLID---------TVFEIGSVGKSSSHGIGNF 360

Query: 379 FAHMTEVSSSKNETWNALKLARESLPLENIKVSPASTTNPGIPPSSLMAFLAKNPQISGV 438
           FAHMTEVSSSKNETWNALK A+ESLP E  K+SPASTTNPGIPPSSLMAFL KN  +SGV
Sbjct: 361 FAHMTEVSSSKNETWNALKHAQESLPFEITKISPASTTNPGIPPSSLMAFLKKNSHVSGV 420

Query: 439 VLDDFDTGFTNQFYQSHLDDLHNINSSAIEAAALLVARTLYILAINKNELSSSVLTAIKV 498
           VL+DFDTGFTNQFYQSHLDDL+NINSSAIEAAALLVARTLYILA NK EL SS +T+IKV
Sbjct: 421 VLEDFDTGFTNQFYQSHLDDLYNINSSAIEAAALLVARTLYILATNKKELISSAITSIKV 480

Query: 499 NTSLVEELIGCLLNCDPGLSCELVKRYITPSSVCPNHYVGVILDEPSSAPYPDYVHDVSR 558
           NTSLVEELIGCLLNCDPGLSCELVKRYI+P+SVCPNHYVGVILDEPSS PYP YVHDVSR
Sbjct: 481 NTSLVEELIGCLLNCDPGLSCELVKRYISPTSVCPNHYVGVILDEPSSTPYPGYVHDVSR 540

Query: 559 FVWNFLADRTSIPKENTSSVCSQNCDDRSEVCIGAETGKGTCVISTTRYIPAYSTRLKFE 618
           FVWNFLADRTSI KENTSS CS NC+D+SEVCIGAE GKGTCVISTTRY+PAYSTRLK+E
Sbjct: 541 FVWNFLADRTSISKENTSSACSPNCNDKSEVCIGAEIGKGTCVISTTRYVPAYSTRLKYE 600

Query: 619 SGYWSVLPPNSSDHLGAVDPVWTESNWNTIGLRIYTIQAAAYDRFVLLGGITTTILAYFA 678
           SG W VLP NSSD +GAVDPVWTESNWNTIGLR+YTIQ  AYDRFVLLGGITTTILAYFA
Sbjct: 601 SGSWYVLPSNSSDPMGAVDPVWTESNWNTIGLRMYTIQTTAYDRFVLLGGITTTILAYFA 660

Query: 679 IVAVRSSIIKALKRD 693
           IVAVR SIIKALKRD
Sbjct: 661 IVAVRGSIIKALKRD 665

BLAST of Cmc06g0163481 vs. TAIR 10
Match: AT3G52640.1 (Zn-dependent exopeptidases superfamily protein )

HSP 1 Score: 828.2 bits (2138), Expect = 5.0e-240
Identity = 418/677 (61.74%), Postives = 515/677 (76.07%), Query Frame = 0

Query: 19  MSSQFLYLLLFLTSLRLSSSDE-QKMESVPDLQNSMYLAVDGYPCIRLLNLSGEIGCSNP 78
           +S  F  +LL +  L LS +DE   +ESVPDLQ  MY+AVDG+PC+RLLNLSGEIGCSNP
Sbjct: 9   LSIAFTLVLLSILPLHLSLADEITSIESVPDLQKLMYVAVDGFPCVRLLNLSGEIGCSNP 68

Query: 79  GREKVVLPMINFKDADEILEPSAVLVSMDAISSFFTRLQDDSHFANNVGGVLIEPGTGIQ 138
           G  KVV P+I  KD  ++++P  +LV+ D +  FFTR+  D  FA+ +GGVL+E G+  Q
Sbjct: 69  GINKVVAPIIKLKDVKDLVQPHTILVTADEMEDFFTRVSTDLSFASKIGGVLVESGSNFQ 128

Query: 139 NRTEGFSPAQKFPQAKFAPYKKNDYEWNPIGSGIMWNRYNFPVFLISESSISSIQEAASK 198
            + +GFSP ++FPQA+F+PY+  +Y+WN   S IMW  YNFPV+L+SES IS++ E  SK
Sbjct: 129 QKLKGFSPDKRFPQAQFSPYENVEYKWNSAASSIMWRNYNFPVYLLSESGISAVHEILSK 188

Query: 199 NVKSKKDYVSNVAEFDLVMQTTKAGTHSSMSCLKEETCLPLGGYSVWSSLPPINTSSSDQ 258
                  Y S+VAEF++VM+TTKAGTH+S +CL+E TCLPLGGYSVWSSLPPI+ SSS+ 
Sbjct: 189 KKMKHGTYTSDVAEFNMVMETTKAGTHNSEACLQEGTCLPLGGYSVWSSLPPISVSSSNN 248

Query: 259 SKPVILTVASMDSASFFRDKSIGADSPISGLIALLAAVDALSHVDGLDDLHKQLVFVVFT 318
            KPV+LTVASMD+ASFFRDKS GADSPISGL+ALL AVDALS VDG+ +L KQLVF+V T
Sbjct: 249 RKPVVLTVASMDTASFFRDKSFGADSPISGLVALLGAVDALSRVDGISNLKKQLVFLVLT 308

Query: 319 GESWGYLGSRRFLLELDLQSDAVSGLSNRLIDMEELVVVLRQVFEIGSVGKSSNHGSGNF 378
           GE+WGYLGSRRFL ELDL SDAV+GLSN  I+          V EIGSVGK  + G   F
Sbjct: 309 GETWGYLGSRRFLHELDLHSDAVAGLSNTSIE---------TVLEIGSVGKGLSGGINTF 368

Query: 379 FAHMTEVSSSKNETWNALKLARESLPLENIKVSPASTTNPGIPPSSLMAFLAKNPQISGV 438
           FAH T VSS  N T +ALK+A++SL  +NIK+  A T NPGIPPSSLMAF+ KNPQ S V
Sbjct: 369 FAHKTRVSSVTNMTLDALKIAQDSLASKNIKILSADTANPGIPPSSLMAFMRKNPQTSAV 428

Query: 439 VLDDFDTGFTNQFYQSHLDDLHNINSSAIEAAALLVARTLYILAINKNELSSSVLTAIKV 498
           VL+DFDT F N+FY SHLDDL NINSS++ AAA +VARTLYILA +  + S+S L +I V
Sbjct: 429 VLEDFDTNFVNKFYHSHLDDLSNINSSSVVAAASVVARTLYILASDNKDTSNSALGSIHV 488

Query: 499 NTSLVEELIGCLLNCDPGLSCELVKRYITPSSVCPNHYVGVILDEPSSAPYPDYVHDVSR 558
           N S VEEL+ CLL C+PGLSC LVK YI+P++ CP +Y GVIL EPSS PY  YV DVSR
Sbjct: 489 NASFVEELLTCLLACEPGLSCNLVKDYISPTNTCPGNYAGVILGEPSSKPYLGYVGDVSR 548

Query: 559 FVWNFLADRTSIPKENTSSVCSQN-CDDRSEVCIGAETGK-GTCVISTTRYIPAYSTRLK 618
           F+WNFLAD+TS+ K NT+SVCS+  C    EVCI AE+ K GTCV+STTRY+PAYSTRLK
Sbjct: 549 FLWNFLADKTSVQKGNTTSVCSKGVCSKTDEVCIKAESNKEGTCVVSTTRYVPAYSTRLK 608

Query: 619 FESGYWSVLPPNSSDHLGAVDPVWTESNWNTIGLRIYTIQAAAYDRFVLLGGITTTILAY 678
           +  G W++LP NSSD +G VDPVWTESNW+T+ + +YT+Q +AYD  VL+ GIT T LAY
Sbjct: 609 YNDGAWTILPQNSSDSMGMVDPVWTESNWDTLRVHVYTVQHSAYDNAVLVAGITVTTLAY 668

Query: 679 FAIVAVRSSIIKALKRD 693
             I+A +S I KALK+D
Sbjct: 669 IGILAAKSIITKALKQD 676

BLAST of Cmc06g0163481 vs. TAIR 10
Match: AT3G52640.2 (Zn-dependent exopeptidases superfamily protein )

HSP 1 Score: 812.4 bits (2097), Expect = 2.8e-235
Identity = 418/706 (59.21%), Postives = 515/706 (72.95%), Query Frame = 0

Query: 19  MSSQFLYLLLFLTSLRLSSSDE-QKMESVPDLQNSMYLAVDGYPCIRLLNLSGEIGCSNP 78
           +S  F  +LL +  L LS +DE   +ESVPDLQ  MY+AVDG+PC+RLLNLSGEIGCSNP
Sbjct: 9   LSIAFTLVLLSILPLHLSLADEITSIESVPDLQKLMYVAVDGFPCVRLLNLSGEIGCSNP 68

Query: 79  GREKVVLPMINFKDADEILEPSAVLVSMDAISSFFTRLQDDSHFANNVGGVLIEPGTGIQ 138
           G  KVV P+I  KD  ++++P  +LV+ D +  FFTR+  D  FA+ +GGVL+E G+  Q
Sbjct: 69  GINKVVAPIIKLKDVKDLVQPHTILVTADEMEDFFTRVSTDLSFASKIGGVLVESGSNFQ 128

Query: 139 NRTEGFSPAQKFPQAKFAPYKKNDYEWNPIGSGIMWNRYNFPVFLISESSISSIQEAASK 198
            + +GFSP ++FPQA+F+PY+  +Y+WN   S IMW  YNFPV+L+SES IS++ E  SK
Sbjct: 129 QKLKGFSPDKRFPQAQFSPYENVEYKWNSAASSIMWRNYNFPVYLLSESGISAVHEILSK 188

Query: 199 NVKSKKDYVSNVAEFDLVMQTTKAGTHSSMSCLKEETCLPLGGYSVWSSLPPINTSSSDQ 258
                  Y S+VAEF++VM+TTKAGTH+S +CL+E TCLPLGGYSVWSSLPPI+ SSS+ 
Sbjct: 189 KKMKHGTYTSDVAEFNMVMETTKAGTHNSEACLQEGTCLPLGGYSVWSSLPPISVSSSNN 248

Query: 259 SKPVILTVASMDSASFFRDKSIGADSPISGLIALLAAVDALSHVDGLDDLHKQLVFVVFT 318
            KPV+LTVASMD+ASFFRDKS GADSPISGL+ALL AVDALS VDG+ +L KQLVF+V T
Sbjct: 249 RKPVVLTVASMDTASFFRDKSFGADSPISGLVALLGAVDALSRVDGISNLKKQLVFLVLT 308

Query: 319 GESWGYLGSRRFLLELDLQSDAVSGLSNRLIDMEELVVVLRQVFEIGSVGKSSNHGSGNF 378
           GE+WGYLGSRRFL ELDL SDAV+GLSN  I+          V EIGSVGK  + G   F
Sbjct: 309 GETWGYLGSRRFLHELDLHSDAVAGLSNTSIE---------TVLEIGSVGKGLSGGINTF 368

Query: 379 FAHMTEVSSSKNETWNALKLARESLPLENIKVSPASTTNPGIPPSSLMAFLAKNPQISGV 438
           FAH T VSS  N T +ALK+A++SL  +NIK+  A T NPGIPPSSLMAF+ KNPQ S V
Sbjct: 369 FAHKTRVSSVTNMTLDALKIAQDSLASKNIKILSADTANPGIPPSSLMAFMRKNPQTSAV 428

Query: 439 VLDDFDTGFTNQFYQSHLDDL-----------------------------HNINSSAIEA 498
           VL+DFDT F N+FY SHLDDL                              NINSS++ A
Sbjct: 429 VLEDFDTNFVNKFYHSHLDDLCKKSHSLSFSSFRSKPHFALLIPFWCCIAANINSSSVVA 488

Query: 499 AALLVARTLYILAINKNELSSSVLTAIKVNTSLVEELIGCLLNCDPGLSCELVKRYITPS 558
           AA +VARTLYILA +  + S+S L +I VN S VEEL+ CLL C+PGLSC LVK YI+P+
Sbjct: 489 AASVVARTLYILASDNKDTSNSALGSIHVNASFVEELLTCLLACEPGLSCNLVKDYISPT 548

Query: 559 SVCPNHYVGVILDEPSSAPYPDYVHDVSRFVWNFLADRTSIPKENTSSVCSQN-CDDRSE 618
           + CP +Y GVIL EPSS PY  YV DVSRF+WNFLAD+TS+ K NT+SVCS+  C    E
Sbjct: 549 NTCPGNYAGVILGEPSSKPYLGYVGDVSRFLWNFLADKTSVQKGNTTSVCSKGVCSKTDE 608

Query: 619 VCIGAETGK-GTCVISTTRYIPAYSTRLKFESGYWSVLPPNSSDHLGAVDPVWTESNWNT 678
           VCI AE+ K GTCV+STTRY+PAYSTRLK+  G W++LP NSSD +G VDPVWTESNW+T
Sbjct: 609 VCIKAESNKEGTCVVSTTRYVPAYSTRLKYNDGAWTILPQNSSDSMGMVDPVWTESNWDT 668

Query: 679 IGLRIYTIQAAAYDRFVLLGGITTTILAYFAIVAVRSSIIKALKRD 693
           + + +YT+Q +AYD  VL+ GIT T LAY  I+A +S I KALK+D
Sbjct: 669 LRVHVYTVQHSAYDNAVLVAGITVTTLAYIGILAAKSIITKALKQD 705

BLAST of Cmc06g0163481 vs. TAIR 10
Match: AT3G52640.3 (Zn-dependent exopeptidases superfamily protein )

HSP 1 Score: 697.6 bits (1799), Expect = 1.0e-200
Identity = 365/619 (58.97%), Postives = 447/619 (72.21%), Query Frame = 0

Query: 19  MSSQFLYLLLFLTSLRLSSSDE-QKMESVPDLQNSMYLAVDGYPCIRLLNLSGEIGCSNP 78
           +S  F  +LL +  L LS +DE   +ESVPDLQ  MY+AVDG+PC+RLLNLSGEIGCSNP
Sbjct: 9   LSIAFTLVLLSILPLHLSLADEITSIESVPDLQKLMYVAVDGFPCVRLLNLSGEIGCSNP 68

Query: 79  GREKVVLPMINFKDADEILEPSAVLVSMDAISSFFTRLQDDSHFANNVGGVLIEPGTGIQ 138
           G  KVV P+I  KD  ++++P  +LV+ D +  FFTR+  D  FA+ +GGVL+E G+  Q
Sbjct: 69  GINKVVAPIIKLKDVKDLVQPHTILVTADEMEDFFTRVSTDLSFASKIGGVLVESGSNFQ 128

Query: 139 NRTEGFSPAQKFPQAKFAPYKKNDYEWNPIGSGIMWNRYNFPVFLISESSISSIQEAASK 198
            + +GFSP ++FPQA+F+PY+  +Y+WN   S IMW  YNFPV+L+SES IS++ E  SK
Sbjct: 129 QKLKGFSPDKRFPQAQFSPYENVEYKWNSAASSIMWRNYNFPVYLLSESGISAVHEILSK 188

Query: 199 NVKSKKDYVSNVAEFDLVMQTTKAGTHSSMSCLKEETCLPLGGYSVWSSLPPINTSSSDQ 258
                  Y S+VAEF++VM+TTKAGTH+S +CL+E TCLPLGGYSVWSSLPPI+ SSS+ 
Sbjct: 189 KKMKHGTYTSDVAEFNMVMETTKAGTHNSEACLQEGTCLPLGGYSVWSSLPPISVSSSNN 248

Query: 259 SKPVILTVASMDSASFFRDKSIGADSPISGLIALLAAVDALSHVDGLDDLHKQLVFVVFT 318
            KPV+LTVASMD+ASFFRDKS GADSPISGL+ALL AVDALS VDG+ +L KQLVF+V T
Sbjct: 249 RKPVVLTVASMDTASFFRDKSFGADSPISGLVALLGAVDALSRVDGISNLKKQLVFLVLT 308

Query: 319 GESWGYLGSRRFLLELDLQSDAVSGLSNRLIDMEELVVVLRQVFEIGSVGKSSNHGSGNF 378
           GE+WGYLGSRRFL ELDL SDAV+GLSN  I+          V EIGSVGK  + G   F
Sbjct: 309 GETWGYLGSRRFLHELDLHSDAVAGLSNTSIE---------TVLEIGSVGKGLSGGINTF 368

Query: 379 FAHMTEVSSSKNETWNALKLARESLPLENIKVSPASTTNPGIPPSSLMAFLAKNPQISGV 438
           FAH T VSS  N T +ALK+A++SL  +NIK+  A T NPGIPPSSLMAF+ KNPQ S V
Sbjct: 369 FAHKTRVSSVTNMTLDALKIAQDSLASKNIKILSADTANPGIPPSSLMAFMRKNPQTSAV 428

Query: 439 VLDDFDTGFTNQFYQSHLDDL-----------------------------HNINSSAIEA 498
           VL+DFDT F N+FY SHLDDL                              NINSS++ A
Sbjct: 429 VLEDFDTNFVNKFYHSHLDDLCKKSHSLSFSSFRSKPHFALLIPFWCCIAANINSSSVVA 488

Query: 499 AALLVARTLYILAINKNELSSSVLTAIKVNTSLVEELIGCLLNCDPGLSCELVKRYITPS 558
           AA +VARTLYILA +  + S+S L +I VN S VEEL+ CLL C+PGLSC LVK YI+P+
Sbjct: 489 AASVVARTLYILASDNKDTSNSALGSIHVNASFVEELLTCLLACEPGLSCNLVKDYISPT 548

Query: 559 SVCPNHYVGVILDEPSSAPYPDYVHDVSRFVWNFLADRTSIPKENTSSVCSQN-CDDRSE 606
           + CP +Y GVIL EPSS PY  YV DVSRF+WNFLAD+TS+ K NT+SVCS+  C    E
Sbjct: 549 NTCPGNYAGVILGEPSSKPYLGYVGDVSRFLWNFLADKTSVQKGNTTSVCSKGVCSKTDE 608