Cmc06g0158781 (gene) Melon (Charmono) v1.1

Overview
NameCmc06g0158781
Typegene
OrganismCucumis melo L. var. cantalupensis cv. Charmono (Melon (Charmono) v1.1)
Descriptioncytochrome P450 714C2-like
LocationCMiso1.1chr06: 7195613 .. 7200263 (+)
RNA-Seq ExpressionCmc06g0158781
SyntenyCmc06g0158781
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CCACATTAGCACATACACACACACAAATTTTAAAACACAGAACTCTCTCTCTCCTTCTCTCTCTCGTTTTGTTTTCATTTTTATAAGTAGATTAAAGTACTTTTTTCTTTATTTTAATTACAAAAGATGGAGTTGATTTGTGTGTTGATTATGTTAATCAAGTGGATGATTAGGGTTTCAATTATGGTATTGTTTGGGAAAGTGGTGATTTCATGCTGGATTTTGCCAATTTTGGCTCATAGAAAATTGAAGAGAAATGGATTTGAAGGCCCTTCTCCTAGTTTCCCTCTTGGGAATATTACAGAAATGAAGAAGATAATGAAAGCTGCTGCTGCTGCTACTGCTGTCACTAATGGATCATCATCAAATAATTTGTCCCATGATATTCATTCAACTATTTTCCCTCACTTTGCTCAATGGCAGAATTCTTATGGTAATTAATCAAACAACCCCCCCCCCCCCCATATTTTTTAAATAGCAATGATCTCTTGTTACTTTTATGATTGATTATTATGCATGAATGATAATATATATTATATAGTTAGTGGATAAAAGATTATATTATTAGTAAAATACCTAAGGGTAAAGTGTATTTCAACTACTATGTTTAAGTATAATTTTACTTATTTTTAAAATATTTTATCAATTTGCTTAAAAATGGGTGCATCAATATTTTTGCTCATTTTTTTATGATATTGTTTTCATTTGTATTATATAAATAAGAGCTAACATAATGTCACTTCAACTTCACTGTGCATGCAAGCACACATAGACATATAGACATGTGCATATTATAATACTATGTTGTTTATCATTTTTAAAAGTTTAAAAAATGGACTATATCTGAGCTGCATCTTTATCCTTATTTAGTTATTTGTGCAGCTTGGACAGTTATTGTCCATTAAAGGGATGCATGAGATTAGGAACTAGCTAATAACCTACCTTATATTTATAATCAATGCCATTAAAATTTTAGGCTCTCAACTTTTTAATATCATGCGTTTAGAATTTTGAATGGTAAATTTCAATATTGTAGCTGATAAATAAAAACTTTTACTTTGTTTTCCTAAAATACTAATAAGAAAATTAATAGAAATGCACCTTAAGAATATAGTTTCGTTTTCGTGTTATTACGACAGGGAAGAAGTTTGTATATTGGTTGGGGACAGAGCCATTTCTGTACATAGCAGATCCACAGCTTGTGAAGAAGATATCCGAAGCTGTATTGGGTAAGAGCTGGGGGAAGCCAGCAGTCTTTAGAAACGACCGAGAACCCATGTTTGGCGATGGCCTTGTCATGACTGAGGGAGACAACTGGATTCGCCATCGCCATATCCTCACTCCTGCCTTCAACCCTACCAATCTGAAGGTAATTTGACTCATATTTCACTCCTAATCTAAGTTATCTATGTAACCAATATTTATACTATTCTTAGTTCAACAATACTTATAGAAGATTCGACTAACTTTTCATCTTAGATTATATTTTATATGAGTTGAGTTATATTTCAGCCAACTGAGGTATTAGATTTGCACAATCCAACATTTTGTCTGATCTCTAAATTCAAACTTGTTTAAAGCTATGGTGAAATGAGTATAAATCTTCCAACAAATTAGGTAATAGAAATATTAGTATTATTAAGAAAAGACAAATTTTATTTGTTTATTAATATATTGATGGTGGAGATATGACAACATACCATCGGGACCAATAATATCTGTATAATGAATGACCGAATAAATTAGAAGATTAGTCGATGGTTTTGTTGACAAGATTGTAATATTTGTCTCTTTTATAGGAATTAAAAAATATTATATTAAATAACGATTTTTTTAATTTATTTTTTAATACCCATTTCTCTCACCTTCAAGTTAATTTAACCTTTTTCTTTTTTTAGTTTCATAAGGCCACATTTTTTCAGTTTTTAGTATCGTTTATTTTTATTTTTAATTTTCAACATTTTATATTTTTATTATCAATTTTACTATTAAGTTAAAAGTACATTTTATATTGGATTTTTTTAAAAATTATAAAAAATTGACAAAATACTTACATTTTATAAAATAAAATCATTAAAATATAAAATTTATTACACCGATTTTTCTATGAAATGTAATGGTTCTATTTTTTATAATTTTCCTTTTATTACTTTTATTTTTAGTGTTAACTTGGAATTGATTAATTTCAAATAATTGTATTTATCAAATAAAAATAAAACTCAAAATTTATAAATAAAAATATGACATTTTTGAACCTAAGAAATCATCCAATAAAAACTAAACACAAAAATCATGAGCTTTTTAATAATTTAAAATCTAATAGAAAAAATAGATAATTTAAGAATTCTCATCTGTAAACATACATTATCATCTTCTTTTATGTTAATAAAATTTCCATGAATGAATATATAGAATCTTATATATCAATAAAAAGAATCTTATAAACAATATAGGATAGGAAATTGAACTTTTAATTTAAAAATAAAAAATTATAATTTTCTACCCTGTCTCTTATTTAATATAGCAAAATTTTATTTCTATTATTGATATAATCTAAAATTTTATTTTTATCATTTTTGTTATCATATATATTTAAATGCAAAAGAAAATGTGAATTAATTAATTCAGGCAATGACAAAATTCATGGTGGAGTCGACGACAAAAATGCTAGAAGAATGGAGAAGCCTAGTAAAATCAGGGCAGTCAGAAATAGAGGTGGAAAGACAAATAACCGCCACCGCGGGCGAAATAATAGCAAAAGCAAGCTTTGGAATATCCAACTGCGGCGGCGGTGGAGAAGCATTGAACAACCTGAGAGCATTACAGTTAACTCTTTTCAAGAACAGTAGCTACGTGGGGGTCCCATTCAGCAGCCTATTTCTTTACCCAGCCCGAACCCTAGCCGCCAAGCGACTGGGAGCTCAAATCGACCGCCTGTTTTTGTCCATCATATCTAATAGGAAAATGTCGGGTTCGGGATCGGGATATTCGGATTTGCTGAGCCGGTTGATTGGGAGTGAGTATACGGGCGGGGAGGCGCTGAGTCCGAGAGAAGTGGTGGATGAGTGTAAGACTTTTTTCTTTGGGGGACATGAGACCACGGCGCTGGCTATTAGTTGGACGTTGCTGTTGTTGGCGACGTCTCCCGATTGGCAAAGGATTTTGCGAGAGGAGATTAAAGAAGTGGTTGGTGATAAAGATATTGATTTCTCCATGCTTTCTTCCCTCAAAAAGGTTCCCTTTTCTTCTTTTTCAAACTTAAACCAACTAACCTTGGACAAATGCTTGCATTTTACATTATTTACTTCATATTCTCCTATTTTCTTTTCTCTTTGAACCATTCTCCAAAATATACCATTTTTAATTAAAATATTTACATATTTCATAATAAAATCTAAACTTTTACTAATTATAGTTTTCTCTCTTTTTTCTTCATTTTCTTGTTTTTTAAAAATTAAGTTTTTTTTTTATTTATTTTCTTAGGCTGAATTTAGAATTCTTAGTCAAATTAAAAAAAAAATTAAATTTTTTAAAAGCTCCTTTTTTTTTTTTTTTTGTTAGTTTTCAAATTTTAACTTGTTTTTTTAAAATTAGGGATAAGAAATAGATATTAAAATCAACATAAATAAATTACGGGAGAAGAAACTATTTAATAAGCTTAATTTTCACCAAAAATAAAAAATAAAATGGTTATTAAAACAGGATCTTAGTTCTTTAGTTTTTAATTTACTTTTTTAGTAAAAAATATTGATTTTATATGTTATTTGAATTTTTTTTAAAAGAGGTTAATTAACTACAAACCTTTTAGTTTTCAATAACACTCTCTAACTTTTCAATTAGATATAGTGTCCAGCTATCAAAAATTGAAAGTAAGTTTGAAGTTAACTATATATGTCACTTTTTAGAACTTTTGAACATATTTATTACATAATATGTTTGAGATAATGTATTAGAAATGCAGTTCTTAAAATTTATGGGCCCAACATAAAAATTCATACATTATTAAATATGATTAATACTGTGTGGATGCAGATGGGATGGGTATGGAATGAGGTACTTAGACTATACCCCTCAGCTCCAAACATACAAAGACAAGCAAAAGGAGACATTGATTTAGGGGACATAAAGATCCCAAAGGGGACCAACATATGGATAGACGTTGTTGCAATGCATCACGACCCGACCCTATGGGGCGACGACGTTAACGACTTCAATCCTAGCAGGTTCCAACACGACACCATTCATGGCGGATGCAACCACAAAATGGGATATTTGCCTTTCGGCTTTGGAGGGAGAATGTGCATTGGTAGAAACTTATCTTCTATGGAGTATAAGATCGTCCTAACCCTAATTCTTTCTACCTTTTCACTCTCACTTTCCCCCTCTTACTCCCATTCTCCTGCTACTTTACTCTCTTTGAGGCCCGCCCATGGCATTCCCCTCATTCTAACCCCTCTTCAACCTTAAACCTTCTTCACGTTTCCTGCTTCTATTCTTAATTAGCTCAACATATAGTAGTAATATTTGAAGGTGCATGTGAGGTCGATCGAAGGGTATGTTCTTAACTAGTGGAGCTTTATGCGCCCATAATACTTCTTCTTTTCCTTTAGACTACAGTATATGATTCAAAGATATATATATGTACTAAAAT

mRNA sequence

CCACATTAGCACATACACACACACAAATTTTAAAACACAGAACTCTCTCTCTCCTTCTCTCTCTCGTTTTGTTTTCATTTTTATAAGTAGATTAAAGTACTTTTTTCTTTATTTTAATTACAAAAGATGGAGTTGATTTGTGTGTTGATTATGTTAATCAAGTGGATGATTAGGGTTTCAATTATGGTATTGTTTGGGAAAGTGGTGATTTCATGCTGGATTTTGCCAATTTTGGCTCATAGAAAATTGAAGAGAAATGGATTTGAAGGCCCTTCTCCTAGTTTCCCTCTTGGGAATATTACAGAAATGAAGAAGATAATGAAAGCTGCTGCTGCTGCTACTGCTGTCACTAATGGATCATCATCAAATAATTTGTCCCATGATATTCATTCAACTATTTTCCCTCACTTTGCTCAATGGCAGAATTCTTATGGGAAGAAGTTTGTATATTGGTTGGGGACAGAGCCATTTCTGTACATAGCAGATCCACAGCTTGTGAAGAAGATATCCGAAGCTGTATTGGGTAAGAGCTGGGGGAAGCCAGCAGTCTTTAGAAACGACCGAGAACCCATGTTTGGCGATGGCCTTGTCATGACTGAGGGAGACAACTGGATTCGCCATCGCCATATCCTCACTCCTGCCTTCAACCCTACCAATCTGAAGGCAATGACAAAATTCATGGTGGAGTCGACGACAAAAATGCTAGAAGAATGGAGAAGCCTAGTAAAATCAGGGCAGTCAGAAATAGAGGTGGAAAGACAAATAACCGCCACCGCGGGCGAAATAATAGCAAAAGCAAGCTTTGGAATATCCAACTGCGGCGGCGGTGGAGAAGCATTGAACAACCTGAGAGCATTACAGTTAACTCTTTTCAAGAACAGTAGCTACGTGGGGGTCCCATTCAGCAGCCTATTTCTTTACCCAGCCCGAACCCTAGCCGCCAAGCGACTGGGAGCTCAAATCGACCGCCTGTTTTTGTCCATCATATCTAATAGGAAAATGTCGGGTTCGGGATCGGGATATTCGGATTTGCTGAGCCGGTTGATTGGGAGTGAGTATACGGGCGGGGAGGCGCTGAGTCCGAGAGAAGTGGTGGATGAGTGTAAGACTTTTTTCTTTGGGGGACATGAGACCACGGCGCTGGCTATTAGTTGGACGTTGCTGTTGTTGGCGACGTCTCCCGATTGGCAAAGGATTTTGCGAGAGGAGATTAAAGAAGTGGTTGGTGATAAAGATATTGATTTCTCCATGCTTTCTTCCCTCAAAAAGATGGGATGGGTATGGAATGAGGTACTTAGACTATACCCCTCAGCTCCAAACATACAAAGACAAGCAAAAGGAGACATTGATTTAGGGGACATAAAGATCCCAAAGGGGACCAACATATGGATAGACGTTGTTGCAATGCATCACGACCCGACCCTATGGGGCGACGACGTTAACGACTTCAATCCTAGCAGGTTCCAACACGACACCATTCATGGCGGATGCAACCACAAAATGGGATATTTGCCTTTCGGCTTTGGAGGGAGAATGTGCATTGGTAGAAACTTATCTTCTATGGAGTATAAGATCGTCCTAACCCTAATTCTTTCTACCTTTTCACTCTCACTTTCCCCCTCTTACTCCCATTCTCCTGCTACTTTACTCTCTTTGAGGCCCGCCCATGGCATTCCCCTCATTCTAACCCCTCTTCAACCTTAAACCTTCTTCACGTTTCCTGCTTCTATTCTTAATTAGCTCAACATATAGTAGTAATATTTGAAGGTGCATGTGAGGTCGATCGAAGGGTATGTTCTTAACTAGTGGAGCTTTATGCGCCCATAATACTTCTTCTTTTCCTTTAGACTACAGTATATGATTCAAAGATATATATATGTACTAAAAT

Coding sequence (CDS)

ATGGAGTTGATTTGTGTGTTGATTATGTTAATCAAGTGGATGATTAGGGTTTCAATTATGGTATTGTTTGGGAAAGTGGTGATTTCATGCTGGATTTTGCCAATTTTGGCTCATAGAAAATTGAAGAGAAATGGATTTGAAGGCCCTTCTCCTAGTTTCCCTCTTGGGAATATTACAGAAATGAAGAAGATAATGAAAGCTGCTGCTGCTGCTACTGCTGTCACTAATGGATCATCATCAAATAATTTGTCCCATGATATTCATTCAACTATTTTCCCTCACTTTGCTCAATGGCAGAATTCTTATGGGAAGAAGTTTGTATATTGGTTGGGGACAGAGCCATTTCTGTACATAGCAGATCCACAGCTTGTGAAGAAGATATCCGAAGCTGTATTGGGTAAGAGCTGGGGGAAGCCAGCAGTCTTTAGAAACGACCGAGAACCCATGTTTGGCGATGGCCTTGTCATGACTGAGGGAGACAACTGGATTCGCCATCGCCATATCCTCACTCCTGCCTTCAACCCTACCAATCTGAAGGCAATGACAAAATTCATGGTGGAGTCGACGACAAAAATGCTAGAAGAATGGAGAAGCCTAGTAAAATCAGGGCAGTCAGAAATAGAGGTGGAAAGACAAATAACCGCCACCGCGGGCGAAATAATAGCAAAAGCAAGCTTTGGAATATCCAACTGCGGCGGCGGTGGAGAAGCATTGAACAACCTGAGAGCATTACAGTTAACTCTTTTCAAGAACAGTAGCTACGTGGGGGTCCCATTCAGCAGCCTATTTCTTTACCCAGCCCGAACCCTAGCCGCCAAGCGACTGGGAGCTCAAATCGACCGCCTGTTTTTGTCCATCATATCTAATAGGAAAATGTCGGGTTCGGGATCGGGATATTCGGATTTGCTGAGCCGGTTGATTGGGAGTGAGTATACGGGCGGGGAGGCGCTGAGTCCGAGAGAAGTGGTGGATGAGTGTAAGACTTTTTTCTTTGGGGGACATGAGACCACGGCGCTGGCTATTAGTTGGACGTTGCTGTTGTTGGCGACGTCTCCCGATTGGCAAAGGATTTTGCGAGAGGAGATTAAAGAAGTGGTTGGTGATAAAGATATTGATTTCTCCATGCTTTCTTCCCTCAAAAAGATGGGATGGGTATGGAATGAGGTACTTAGACTATACCCCTCAGCTCCAAACATACAAAGACAAGCAAAAGGAGACATTGATTTAGGGGACATAAAGATCCCAAAGGGGACCAACATATGGATAGACGTTGTTGCAATGCATCACGACCCGACCCTATGGGGCGACGACGTTAACGACTTCAATCCTAGCAGGTTCCAACACGACACCATTCATGGCGGATGCAACCACAAAATGGGATATTTGCCTTTCGGCTTTGGAGGGAGAATGTGCATTGGTAGAAACTTATCTTCTATGGAGTATAAGATCGTCCTAACCCTAATTCTTTCTACCTTTTCACTCTCACTTTCCCCCTCTTACTCCCATTCTCCTGCTACTTTACTCTCTTTGAGGCCCGCCCATGGCATTCCCCTCATTCTAACCCCTCTTCAACCTTAA

Protein sequence

MELICVLIMLIKWMIRVSIMVLFGKVVISCWILPILAHRKLKRNGFEGPSPSFPLGNITEMKKIMKAAAAATAVTNGSSSNNLSHDIHSTIFPHFAQWQNSYGKKFVYWLGTEPFLYIADPQLVKKISEAVLGKSWGKPAVFRNDREPMFGDGLVMTEGDNWIRHRHILTPAFNPTNLKAMTKFMVESTTKMLEEWRSLVKSGQSEIEVERQITATAGEIIAKASFGISNCGGGGEALNNLRALQLTLFKNSSYVGVPFSSLFLYPARTLAAKRLGAQIDRLFLSIISNRKMSGSGSGYSDLLSRLIGSEYTGGEALSPREVVDECKTFFFGGHETTALAISWTLLLLATSPDWQRILREEIKEVVGDKDIDFSMLSSLKKMGWVWNEVLRLYPSAPNIQRQAKGDIDLGDIKIPKGTNIWIDVVAMHHDPTLWGDDVNDFNPSRFQHDTIHGGCNHKMGYLPFGFGGRMCIGRNLSSMEYKIVLTLILSTFSLSLSPSYSHSPATLLSLRPAHGIPLILTPLQP
Homology
BLAST of Cmc06g0158781 vs. NCBI nr
Match: XP_008439236.1 (PREDICTED: cytokinin hydroxylase [Cucumis melo])

HSP 1 Score: 1051.6 bits (2718), Expect = 2.3e-303
Identity = 524/525 (99.81%), Postives = 524/525 (99.81%), Query Frame = 0

Query: 1   MELICVLIMLIKWMIRVSIMVLFGKVVISCWILPILAHRKLKRNGFEGPSPSFPLGNITE 60
           MELICVLIMLIKWMIRVSIMVLFGKVVISCWILPILAHRKLKRNGFEGPSPSFPLGNITE
Sbjct: 1   MELICVLIMLIKWMIRVSIMVLFGKVVISCWILPILAHRKLKRNGFEGPSPSFPLGNITE 60

Query: 61  MKKIMKAAAAATAVTNGSSSNNLSHDIHSTIFPHFAQWQNSYGKKFVYWLGTEPFLYIAD 120
           MKKIMKAAAAATAVTNGSSSNNLSHDIHSTIFPHFAQWQNSYGKKFVYWLGTEPFLYIAD
Sbjct: 61  MKKIMKAAAAATAVTNGSSSNNLSHDIHSTIFPHFAQWQNSYGKKFVYWLGTEPFLYIAD 120

Query: 121 PQLVKKISEAVLGKSWGKPAVFRNDREPMFGDGLVMTEGDNWIRHRHILTPAFNPTNLKA 180
           PQLVKKISEAVLGKSWGKPAVFRNDREPMFGDGLVMTEGDNWIRHRHILTPAFNPTNLKA
Sbjct: 121 PQLVKKISEAVLGKSWGKPAVFRNDREPMFGDGLVMTEGDNWIRHRHILTPAFNPTNLKA 180

Query: 181 MTKFMVESTTKMLEEWRSLVKSGQSEIEVERQITATAGEIIAKASFGISNCGGGGEALNN 240
           MTKFMVESTTKMLEEWRSLVKSGQSEIEVERQITATAGEIIAKASFGISNCGGGGEALNN
Sbjct: 181 MTKFMVESTTKMLEEWRSLVKSGQSEIEVERQITATAGEIIAKASFGISNCGGGGEALNN 240

Query: 241 LRALQLTLFKNSSYVGVPFSSLFLYPARTLAAKRLGAQIDRLFLSIISNRKMSGSGSGYS 300
           LRALQLTLFKNSSYVGVPFSSLFLYPARTLAAKRLGAQIDRLFLSIISNRKMSGSGSGYS
Sbjct: 241 LRALQLTLFKNSSYVGVPFSSLFLYPARTLAAKRLGAQIDRLFLSIISNRKMSGSGSGYS 300

Query: 301 DLLSRLIGSEYTGGEALSPREVVDECKTFFFGGHETTALAISWTLLLLATSPDWQRILRE 360
           DLLSRLIGSEYTGGEALSPREVVDECKTFFFGGHETTALAISWTLLLLATSPDWQRILRE
Sbjct: 301 DLLSRLIGSEYTGGEALSPREVVDECKTFFFGGHETTALAISWTLLLLATSPDWQRILRE 360

Query: 361 EIKEVVGDKDIDFSMLSSLKKMGWVWNEVLRLYPSAPNIQRQAKGDIDLGDIKIPKGTNI 420
           EIKEVVGDKDIDFSMLSSLKKMGWVWNEVLRLYPSAPNIQRQAKGDIDLGDIKIPKGTNI
Sbjct: 361 EIKEVVGDKDIDFSMLSSLKKMGWVWNEVLRLYPSAPNIQRQAKGDIDLGDIKIPKGTNI 420

Query: 421 WIDVVAMHHDPTLWGDDVNDFNPSRFQHDTIHGGCNHKMGYLPFGFGGRMCIGRNLSSME 480
           WIDVVAMHHDPTLWGDDVNDFNPSRFQHDTIHGGC HKMGYLPFGFGGRMCIGRNLSSME
Sbjct: 421 WIDVVAMHHDPTLWGDDVNDFNPSRFQHDTIHGGCYHKMGYLPFGFGGRMCIGRNLSSME 480

Query: 481 YKIVLTLILSTFSLSLSPSYSHSPATLLSLRPAHGIPLILTPLQP 526
           YKIVLTLILSTFSLSLSPSYSHSPATLLSLRPAHGIPLILTPLQP
Sbjct: 481 YKIVLTLILSTFSLSLSPSYSHSPATLLSLRPAHGIPLILTPLQP 525

BLAST of Cmc06g0158781 vs. NCBI nr
Match: XP_004151435.2 (cytokinin hydroxylase [Cucumis sativus])

HSP 1 Score: 1004.2 bits (2595), Expect = 4.1e-289
Identity = 495/525 (94.29%), Postives = 506/525 (96.38%), Query Frame = 0

Query: 1   MELICVLIMLIKWMIRVSIMVLFGKVVISCWILPILAHRKLKRNGFEGPSPSFPLGNITE 60
           MELICVL+MLIKWMIRVSIMVLFGKVVISCWILP LAH+KLKRNGFEGPSPSFPLGNITE
Sbjct: 1   MELICVLVMLIKWMIRVSIMVLFGKVVISCWILPTLAHKKLKRNGFEGPSPSFPLGNITE 60

Query: 61  MKKIMKAAAAATAVTNGSSSNNLSHDIHSTIFPHFAQWQNSYGKKFVYWLGTEPFLYIAD 120
           MKKIMKAAAA  A  NGSSSNNLSHDIHSTIFPHFAQWQNSYGKKFVYWLGTEPFLYIAD
Sbjct: 61  MKKIMKAAAAGGAAINGSSSNNLSHDIHSTIFPHFAQWQNSYGKKFVYWLGTEPFLYIAD 120

Query: 121 PQLVKKISEAVLGKSWGKPAVFRNDREPMFGDGLVMTEGDNWIRHRHILTPAFNPTNLKA 180
           PQLVKKISEAVLGKSWGKPAVFRNDREPMFGDGLVMTEGDNWIRHRHILTPAFNP NLKA
Sbjct: 121 PQLVKKISEAVLGKSWGKPAVFRNDREPMFGDGLVMTEGDNWIRHRHILTPAFNPANLKA 180

Query: 181 MTKFMVESTTKMLEEWRSLVKSGQSEIEVERQITATAGEIIAKASFGISNCGGGGEALNN 240
           MTKFMVEST KMLEEWR  VKSGQSEIEVER+ITATAGEIIAKASFGIS CGGGGEALNN
Sbjct: 181 MTKFMVESTKKMLEEWRRQVKSGQSEIEVEREITATAGEIIAKASFGISKCGGGGEALNN 240

Query: 241 LRALQLTLFKNSSYVGVPFSSLFLYPARTLAAKRLGAQIDRLFLSIISNRKMSGSGSGYS 300
           LRALQLTLFKNSSYVGVPFSSL LYPARTLAAKRLGAQIDRLF SIIS+RK+SGSGS YS
Sbjct: 241 LRALQLTLFKNSSYVGVPFSSLVLYPARTLAAKRLGAQIDRLFFSIISDRKISGSGSAYS 300

Query: 301 DLLSRLIGSEYTGGEALSPREVVDECKTFFFGGHETTALAISWTLLLLATSPDWQRILRE 360
           DLLSRLIGSEY GG  LSPREVVDECKTFFFGGHETTALAISWTLLLLATSPDWQ ILR+
Sbjct: 301 DLLSRLIGSEYKGGAGLSPREVVDECKTFFFGGHETTALAISWTLLLLATSPDWQTILRD 360

Query: 361 EIKEVVGDKDIDFSMLSSLKKMGWVWNEVLRLYPSAPNIQRQAKGDIDLGDIKIPKGTNI 420
           EIKEV+GDKDIDFSMLSSLKKMGWVWNEVLRLYPSAPNIQRQAKGDIDLGD+KIPKGTNI
Sbjct: 361 EIKEVIGDKDIDFSMLSSLKKMGWVWNEVLRLYPSAPNIQRQAKGDIDLGDVKIPKGTNI 420

Query: 421 WIDVVAMHHDPTLWGDDVNDFNPSRFQHDTIHGGCNHKMGYLPFGFGGRMCIGRNLSSME 480
           WID+VAMHHDPTLWGDDVN+FNP RFQHDTIHGGCNHKMGYLPFGFGGRMCIGRNLSSME
Sbjct: 421 WIDIVAMHHDPTLWGDDVNEFNPKRFQHDTIHGGCNHKMGYLPFGFGGRMCIGRNLSSME 480

Query: 481 YKIVLTLILSTFSLSLSPSYSHSPATLLSLRPAHGIPLILTPLQP 526
           YKIVLTLILSTFSLSLSPSY+HSPATLLSLRPAHGIPLILTPL P
Sbjct: 481 YKIVLTLILSTFSLSLSPSYTHSPATLLSLRPAHGIPLILTPLHP 525

BLAST of Cmc06g0158781 vs. NCBI nr
Match: KGN57414.2 (hypothetical protein Csa_010508 [Cucumis sativus])

HSP 1 Score: 975.7 bits (2521), Expect = 1.6e-280
Identity = 477/506 (94.27%), Postives = 487/506 (96.25%), Query Frame = 0

Query: 20  MVLFGKVVISCWILPILAHRKLKRNGFEGPSPSFPLGNITEMKKIMKAAAAATAVTNGSS 79
           MVLFGKVVISCWILP LAH+KLKRNGFEGPSPSFPLGNITEMKKIMKAAAA  A  NGSS
Sbjct: 1   MVLFGKVVISCWILPTLAHKKLKRNGFEGPSPSFPLGNITEMKKIMKAAAAGGAAINGSS 60

Query: 80  SNNLSHDIHSTIFPHFAQWQNSYGKKFVYWLGTEPFLYIADPQLVKKISEAVLGKSWGKP 139
           SNNLSHDIHSTIFPHFAQWQNSYGKKFVYWLGTEPFLYIADPQLVKKISEAVLGKSWGKP
Sbjct: 61  SNNLSHDIHSTIFPHFAQWQNSYGKKFVYWLGTEPFLYIADPQLVKKISEAVLGKSWGKP 120

Query: 140 AVFRNDREPMFGDGLVMTEGDNWIRHRHILTPAFNPTNLKAMTKFMVESTTKMLEEWRSL 199
           AVFRNDREPMFGDGLVMTEGDNWIRHRHILTPAFNP NLKAMTKFMVEST KMLEEWR  
Sbjct: 121 AVFRNDREPMFGDGLVMTEGDNWIRHRHILTPAFNPANLKAMTKFMVESTKKMLEEWRRQ 180

Query: 200 VKSGQSEIEVERQITATAGEIIAKASFGISNCGGGGEALNNLRALQLTLFKNSSYVGVPF 259
           VKSGQSEIEVER+ITATAGEIIAKASFGIS CGGGGEALNNLRALQLTLFKNSSYVGVPF
Sbjct: 181 VKSGQSEIEVEREITATAGEIIAKASFGISKCGGGGEALNNLRALQLTLFKNSSYVGVPF 240

Query: 260 SSLFLYPARTLAAKRLGAQIDRLFLSIISNRKMSGSGSGYSDLLSRLIGSEYTGGEALSP 319
           SSL LYPARTLAAKRLGAQIDRLF SIIS+RK+SGSGS YSDLLSRLIGSEY GG  LSP
Sbjct: 241 SSLVLYPARTLAAKRLGAQIDRLFFSIISDRKISGSGSAYSDLLSRLIGSEYKGGAGLSP 300

Query: 320 REVVDECKTFFFGGHETTALAISWTLLLLATSPDWQRILREEIKEVVGDKDIDFSMLSSL 379
           REVVDECKTFFFGGHETTALAISWTLLLLATSPDWQ ILR+EIKEV+GDKDIDFSMLSSL
Sbjct: 301 REVVDECKTFFFGGHETTALAISWTLLLLATSPDWQTILRDEIKEVIGDKDIDFSMLSSL 360

Query: 380 KKMGWVWNEVLRLYPSAPNIQRQAKGDIDLGDIKIPKGTNIWIDVVAMHHDPTLWGDDVN 439
           KKMGWVWNEVLRLYPSAPNIQRQAKGDIDLGD+KIPKGTNIWID+VAMHHDPTLWGDDVN
Sbjct: 361 KKMGWVWNEVLRLYPSAPNIQRQAKGDIDLGDVKIPKGTNIWIDIVAMHHDPTLWGDDVN 420

Query: 440 DFNPSRFQHDTIHGGCNHKMGYLPFGFGGRMCIGRNLSSMEYKIVLTLILSTFSLSLSPS 499
           +FNP RFQHDTIHGGCNHKMGYLPFGFGGRMCIGRNLSSMEYKIVLTLILSTFSLSLSPS
Sbjct: 421 EFNPKRFQHDTIHGGCNHKMGYLPFGFGGRMCIGRNLSSMEYKIVLTLILSTFSLSLSPS 480

Query: 500 YSHSPATLLSLRPAHGIPLILTPLQP 526
           Y+HSPATLLSLRPAHGIPLILTPL P
Sbjct: 481 YTHSPATLLSLRPAHGIPLILTPLHP 506

BLAST of Cmc06g0158781 vs. NCBI nr
Match: TYK22959.1 (cytokinin hydroxylase [Cucumis melo var. makuwa])

HSP 1 Score: 941.8 bits (2433), Expect = 2.5e-270
Identity = 465/465 (100.00%), Postives = 465/465 (100.00%), Query Frame = 0

Query: 61  MKKIMKAAAAATAVTNGSSSNNLSHDIHSTIFPHFAQWQNSYGKKFVYWLGTEPFLYIAD 120
           MKKIMKAAAAATAVTNGSSSNNLSHDIHSTIFPHFAQWQNSYGKKFVYWLGTEPFLYIAD
Sbjct: 1   MKKIMKAAAAATAVTNGSSSNNLSHDIHSTIFPHFAQWQNSYGKKFVYWLGTEPFLYIAD 60

Query: 121 PQLVKKISEAVLGKSWGKPAVFRNDREPMFGDGLVMTEGDNWIRHRHILTPAFNPTNLKA 180
           PQLVKKISEAVLGKSWGKPAVFRNDREPMFGDGLVMTEGDNWIRHRHILTPAFNPTNLKA
Sbjct: 61  PQLVKKISEAVLGKSWGKPAVFRNDREPMFGDGLVMTEGDNWIRHRHILTPAFNPTNLKA 120

Query: 181 MTKFMVESTTKMLEEWRSLVKSGQSEIEVERQITATAGEIIAKASFGISNCGGGGEALNN 240
           MTKFMVESTTKMLEEWRSLVKSGQSEIEVERQITATAGEIIAKASFGISNCGGGGEALNN
Sbjct: 121 MTKFMVESTTKMLEEWRSLVKSGQSEIEVERQITATAGEIIAKASFGISNCGGGGEALNN 180

Query: 241 LRALQLTLFKNSSYVGVPFSSLFLYPARTLAAKRLGAQIDRLFLSIISNRKMSGSGSGYS 300
           LRALQLTLFKNSSYVGVPFSSLFLYPARTLAAKRLGAQIDRLFLSIISNRKMSGSGSGYS
Sbjct: 181 LRALQLTLFKNSSYVGVPFSSLFLYPARTLAAKRLGAQIDRLFLSIISNRKMSGSGSGYS 240

Query: 301 DLLSRLIGSEYTGGEALSPREVVDECKTFFFGGHETTALAISWTLLLLATSPDWQRILRE 360
           DLLSRLIGSEYTGGEALSPREVVDECKTFFFGGHETTALAISWTLLLLATSPDWQRILRE
Sbjct: 241 DLLSRLIGSEYTGGEALSPREVVDECKTFFFGGHETTALAISWTLLLLATSPDWQRILRE 300

Query: 361 EIKEVVGDKDIDFSMLSSLKKMGWVWNEVLRLYPSAPNIQRQAKGDIDLGDIKIPKGTNI 420
           EIKEVVGDKDIDFSMLSSLKKMGWVWNEVLRLYPSAPNIQRQAKGDIDLGDIKIPKGTNI
Sbjct: 301 EIKEVVGDKDIDFSMLSSLKKMGWVWNEVLRLYPSAPNIQRQAKGDIDLGDIKIPKGTNI 360

Query: 421 WIDVVAMHHDPTLWGDDVNDFNPSRFQHDTIHGGCNHKMGYLPFGFGGRMCIGRNLSSME 480
           WIDVVAMHHDPTLWGDDVNDFNPSRFQHDTIHGGCNHKMGYLPFGFGGRMCIGRNLSSME
Sbjct: 361 WIDVVAMHHDPTLWGDDVNDFNPSRFQHDTIHGGCNHKMGYLPFGFGGRMCIGRNLSSME 420

Query: 481 YKIVLTLILSTFSLSLSPSYSHSPATLLSLRPAHGIPLILTPLQP 526
           YKIVLTLILSTFSLSLSPSYSHSPATLLSLRPAHGIPLILTPLQP
Sbjct: 421 YKIVLTLILSTFSLSLSPSYSHSPATLLSLRPAHGIPLILTPLQP 465

BLAST of Cmc06g0158781 vs. NCBI nr
Match: KAA0033659.1 (cytokinin hydroxylase [Cucumis melo var. makuwa])

HSP 1 Score: 938.7 bits (2425), Expect = 2.1e-269
Identity = 464/465 (99.78%), Postives = 464/465 (99.78%), Query Frame = 0

Query: 61  MKKIMKAAAAATAVTNGSSSNNLSHDIHSTIFPHFAQWQNSYGKKFVYWLGTEPFLYIAD 120
           MKKIMKAAAAATAVTNGSSSNNLSHDIHSTIFPHFAQWQNSYGKKFVYWLGTEPFLYIAD
Sbjct: 1   MKKIMKAAAAATAVTNGSSSNNLSHDIHSTIFPHFAQWQNSYGKKFVYWLGTEPFLYIAD 60

Query: 121 PQLVKKISEAVLGKSWGKPAVFRNDREPMFGDGLVMTEGDNWIRHRHILTPAFNPTNLKA 180
           PQLVKKISEAVLGKSWGKPAVFRNDREPMFGDGLVMTEGDNWIRHRHILTPAFNPTNLKA
Sbjct: 61  PQLVKKISEAVLGKSWGKPAVFRNDREPMFGDGLVMTEGDNWIRHRHILTPAFNPTNLKA 120

Query: 181 MTKFMVESTTKMLEEWRSLVKSGQSEIEVERQITATAGEIIAKASFGISNCGGGGEALNN 240
           MTKFMVESTTKMLEEWRSLVKSGQSEIEVERQITATAGEIIAKASFGISNCGGGGEALNN
Sbjct: 121 MTKFMVESTTKMLEEWRSLVKSGQSEIEVERQITATAGEIIAKASFGISNCGGGGEALNN 180

Query: 241 LRALQLTLFKNSSYVGVPFSSLFLYPARTLAAKRLGAQIDRLFLSIISNRKMSGSGSGYS 300
           LRALQLTLFKNSSYVGVPFSSLFLYPARTLAAKRLGAQIDRLFLSIISNRKMSGSGSGYS
Sbjct: 181 LRALQLTLFKNSSYVGVPFSSLFLYPARTLAAKRLGAQIDRLFLSIISNRKMSGSGSGYS 240

Query: 301 DLLSRLIGSEYTGGEALSPREVVDECKTFFFGGHETTALAISWTLLLLATSPDWQRILRE 360
           DLLSRLIGSEYTGGEALSPREVVDECKTFFFGGHETTALAISWTLLLLATSPDWQRILRE
Sbjct: 241 DLLSRLIGSEYTGGEALSPREVVDECKTFFFGGHETTALAISWTLLLLATSPDWQRILRE 300

Query: 361 EIKEVVGDKDIDFSMLSSLKKMGWVWNEVLRLYPSAPNIQRQAKGDIDLGDIKIPKGTNI 420
           EIKEVVGDKDIDFSMLSSLKKMGWVWNEVLRLYPSAPNIQRQAKGDIDLGDIKIPKGTNI
Sbjct: 301 EIKEVVGDKDIDFSMLSSLKKMGWVWNEVLRLYPSAPNIQRQAKGDIDLGDIKIPKGTNI 360

Query: 421 WIDVVAMHHDPTLWGDDVNDFNPSRFQHDTIHGGCNHKMGYLPFGFGGRMCIGRNLSSME 480
           WIDVVAMHHDPTLWGDDVNDFNPSRFQHDTIHGGC HKMGYLPFGFGGRMCIGRNLSSME
Sbjct: 361 WIDVVAMHHDPTLWGDDVNDFNPSRFQHDTIHGGCYHKMGYLPFGFGGRMCIGRNLSSME 420

Query: 481 YKIVLTLILSTFSLSLSPSYSHSPATLLSLRPAHGIPLILTPLQP 526
           YKIVLTLILSTFSLSLSPSYSHSPATLLSLRPAHGIPLILTPLQP
Sbjct: 421 YKIVLTLILSTFSLSLSPSYSHSPATLLSLRPAHGIPLILTPLQP 465

BLAST of Cmc06g0158781 vs. ExPASy Swiss-Prot
Match: Q2QYH7 (Cytochrome P450 714C2 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP714C2 PE=2 SV=1)

HSP 1 Score: 326.6 bits (836), Expect = 5.0e-88
Identity = 185/508 (36.42%), Postives = 277/508 (54.53%), Query Frame = 0

Query: 20  MVLFGKVVISCWILPILAHRKLKRNGFEGPSPSFPLGNITEMKKIMKAAAAATAVTNGSS 79
           ++LF  V I  W+ P    +KL+  G  GP PSF  GNI EM++I + A +A     G S
Sbjct: 20  ILLFSYVYIILWLRPERLRQKLRSQGVRGPKPSFLFGNIPEMRRIQQLAKSAHEQEAG-S 79

Query: 80  SNNLSHDIHSTIFPHFAQWQNSYGKKFVYWLGTEPFLYIADPQLVKKISEAVLGKSWGKP 139
           ++  S +  +T+FP+F  W   YG  ++Y  G+   L + DP +VK+++        GKP
Sbjct: 80  TDMFSSNYVATLFPYFLHWSRVYGSIYLYSTGSIQVLNVTDPNMVKELANC-KSLDLGKP 139

Query: 140 AVFRNDREPMFGDGLVMTEGDNWIRHRHILTPAFNPTNLKAMTKFMVESTTKMLEEWRSL 199
              + +R  + G G++ + GD W+  R ++ P      +K M   M+E+   ML  W++ 
Sbjct: 140 CYLQKERGALLGMGILTSNGDLWVHQRKVIAPELFMERVKGMVNLMMEAAMSMLNSWKNE 199

Query: 200 V--KSGQSEIEVERQITATAGEIIAKASFGISNCGGGGEALNNLRALQLTLFKNSSYVGV 259
           V  + G +EI V+  +   + ++I++A FG S+   G E    +R LQ  + K S  +GV
Sbjct: 200 VEDRGGSAEIVVDEFLRTFSADVISRACFG-SSFSEGKEIFIKIRQLQKAMAKQSMLIGV 259

Query: 260 PFSSLFLYPARTLAAKRLGAQIDRLFLSIISNRKMSGSGSGYSDLLSRLI-GSEYTGGEA 319
           P  S +L          L + I  L L+I    +   S S   DLL  +I GS+     +
Sbjct: 260 P-GSRYLPTRSNRGIWNLDSSIRTLILNISKKYEHDSSTSVNKDLLHSIIQGSKDGPFAS 319

Query: 320 LSPRE-VVDECKTFFFGGHETTALAISWTLLLLATSPDWQRILREEIKEVVGDKDIDFSM 379
            +P + +VD CK  +F GHETT+   +W L+LLA+  +WQ   R E  ++   + +DF +
Sbjct: 320 CTPEDFIVDNCKNIYFAGHETTSTTAAWCLMLLASHHEWQSRARVESLDICQGRPLDFDI 379

Query: 380 LSSLKKMGWVWNEVLRLYPSAPNIQRQAKGDIDLGDIKIPKGTNIWIDVVAMHHDPTLWG 439
           L  LKK+  V  E LRLYP A  + R+A  D+ LG I IPKGTNIWI +   H DP++WG
Sbjct: 380 LRKLKKLTMVIQETLRLYPPASFVAREALNDMKLGGIDIPKGTNIWIPIAMAHRDPSVWG 439

Query: 440 DDVNDFNPSRFQHDTIHGGCNHKMGYLPFGFGGRMCIGRNLSSMEYKIVLTLILSTFSLS 499
              + F+P RF +  I G C     Y+PFG G R C G+NL+ +E K+VL+L+LS F   
Sbjct: 440 PSADKFDPDRFANG-IAGACKPPHMYMPFGVGVRTCAGQNLAMVELKVVLSLLLSKFEFK 499

Query: 500 LSPSYSHSPATLLSLRPAHGIPLILTPL 524
           LSP+Y H PA  L++ P  G+PLI   L
Sbjct: 500 LSPNYVHCPAFRLTIEPGKGVPLIFREL 522

BLAST of Cmc06g0158781 vs. ExPASy Swiss-Prot
Match: Q9ZW95 (Cytokinin hydroxylase OS=Arabidopsis thaliana OX=3702 GN=CYP735A2 PE=1 SV=1)

HSP 1 Score: 322.0 bits (824), Expect = 1.2e-86
Identity = 191/530 (36.04%), Postives = 294/530 (55.47%), Query Frame = 0

Query: 6   VLIMLIKWMIRVSIMVLFGKVV---ISCWIL-PILAHRKLKRNGFEGPSPSFPLGNITEM 65
           +++ L+   + V +M L  +V+   I C+ L P    + ++R G  GP P    GNI ++
Sbjct: 1   MMVTLVLKYVLVIVMTLILRVLYDSICCYFLTPRRIKKFMERQGITGPKPRLLTGNIIDI 60

Query: 66  KKIMKAAAAATAVTNGSSSNNLSHDIHSTIFPHFAQWQNSYGKKFVYWLGTEPFLYIADP 125
            K++  +A+       +  +++ H+I   + PH+  W   YGK+F+ W GTEP L + + 
Sbjct: 61  SKMLSHSAS-------NDCSSIHHNIVPRLLPHYVSWSKQYGKRFIMWNGTEPRLCLTET 120

Query: 126 QLVKKI---SEAVLGKSWGKPAVFRNDREPMFGDGLVMTEGDNWIRHRHILTPAFNPTNL 185
           +++K++      V GKSW    + +   +   G GL+M  G+ W   RH+  PAF    L
Sbjct: 121 EMIKELLTKHNPVTGKSW----LQQQGTKGFIGRGLLMANGEAWHHQRHMAAPAFTRDRL 180

Query: 186 KAMTKFMVESTTKMLEEWRSLVKSGQSEIEVERQITATAGEIIAKASFGISNCGGGGEAL 245
           K   K MVE T  M E  R   K    E+E+  ++     +II++  FG S+C  G E  
Sbjct: 181 KGYAKHMVECTKMMAERLR---KEVGEEVEIGEEMRRLTADIISRTEFG-SSCDKGKELF 240

Query: 246 NNLRALQLTLFKNSSYVGVPFSSLFLYPARTLAAKRLGAQIDRLFLSIISNRKMS---GS 305
           + L  LQ    + + ++  P  S FL        K L  +++RL + II +RK S   G 
Sbjct: 241 SLLTVLQRLCAQATRHLCFP-GSRFLPSKYNREIKSLKTEVERLLMEIIDSRKDSVEIGR 300

Query: 306 GSGY-SDLLSRLIGSEYTGGEALSPREVVDECKTFFFGGHETTALAISWTLLLLATSPDW 365
            S Y  DLL  L+    +    L+ + ++DECKTFFF GHETT+L ++WTL+LLA +P W
Sbjct: 301 SSSYGDDLLGLLLNQMDSNKNNLNVQMIMDECKTFFFTGHETTSLLLTWTLMLLAHNPTW 360

Query: 366 QRILREEIKEVVGDKDI-DFSMLSSLKKMGWVWNEVLRLYPSAPNIQRQAKGDIDLGDIK 425
           Q  +R+E+++V G   +     LSSL  +  V NE LRLYP A  + R A  DI LGD+ 
Sbjct: 361 QDNVRDEVRQVCGQDGVPSVEQLSSLTSLNKVINESLRLYPPATLLPRMAFEDIKLGDLI 420

Query: 426 IPKGTNIWIDVVAMHHDPTLWGDDVNDFNPSRFQHDTIHGGCNHKMGYLPFGFGGRMCIG 485
           IPKG +IWI V+A+HH   LWG+D N+FNP RF   +      H   ++PF  G R CIG
Sbjct: 421 IPKGLSIWIPVLAIHHSNELWGEDANEFNPERFTTRSF-ASSRH---FMPFAAGPRNCIG 480

Query: 486 RNLSSMEYKIVLTLILSTFSLSLSPSYSHSPATLLSLRPAHGIPLILTPL 524
           +  + ME KI+L +++S FS ++S +Y H+P  +L+++P +G+ L+L PL
Sbjct: 481 QTFAMMEAKIILAMLVSKFSFAISENYRHAPIVVLTIKPKYGVQLVLKPL 510

BLAST of Cmc06g0158781 vs. ExPASy Swiss-Prot
Match: Q9FF18 (Cytokinin hydroxylase OS=Arabidopsis thaliana OX=3702 GN=CYP735A1 PE=1 SV=1)

HSP 1 Score: 315.5 bits (807), Expect = 1.2e-84
Identity = 189/536 (35.26%), Postives = 290/536 (54.10%), Query Frame = 0

Query: 3   LICVLIMLIKWMIRVSIMVLFGKVVISC-WILPILAHRKLKRNGFEGPSPSFPLGNITEM 62
           L+ +L  L+   +   + VL+    ISC W+ P    + +++ G  GP P    GNI E+
Sbjct: 2   LLTILKSLLVIFVTTILRVLYD--TISCYWLTPRRIKKIMEQQGVTGPKPRPLTGNILEI 61

Query: 63  KKIMKAAAAATAVTNGSSSNNLSHDIHSTIFPHFAQWQNSYGKKFVYWLGTEPFLYIADP 122
             ++  +A+          +++ HDI   + PH+  W   YGK+F+ W GT+P L + + 
Sbjct: 62  SAMVSQSAS-------KDCDSIHHDIVGRLLPHYVAWSKQYGKRFIVWNGTDPRLCLTET 121

Query: 123 QLVKKI---SEAVLGKSWGKPAVFRNDREPMFGDGLVMTEGDNWIRHRHILTPAFNPTNL 182
           +L+K++      V G+SW +    +N      G GL+M  G +W   RH+  PAF    L
Sbjct: 122 ELIKELLMKHNGVSGRSWLQQQGTKN----FIGRGLLMANGQDWHHQRHLAAPAFTGERL 181

Query: 183 KAMTKFMVESTTKMLEEWRSLVKSGQSEIEVERQITATAGEIIAKASFGISNCGGGGEAL 242
           K   + MVE T+K++E  R  V  G +E+E+  ++     +II++  FG S+   G E  
Sbjct: 182 KGYARHMVECTSKLVERLRKEVGEGANEVEIGEEMHKLTADIISRTKFG-SSFEKGKELF 241

Query: 243 NNLRALQLTLFKNSSYVGVPFSSLFLYPARTLAAKRLGAQIDRLFLSIISNRK----MSG 302
           N+L  LQ    + + ++  P  S FL        K L  +++RL + II +R+    M  
Sbjct: 242 NHLTVLQRRCAQATRHLCFP-GSRFLPSKYNREIKSLKKEVERLLIEIIQSRRDCAEMGR 301

Query: 303 SGSGYSDLLSRLIG----SEYTGGEALSPREVVDECKTFFFGGHETTALAISWTLLLLAT 362
           S +   DLL  L+      +       + + ++DECKTFFF GHETTAL ++WT +LLA 
Sbjct: 302 SSTHGDDLLGLLLNEMDIDKNNNNNNNNLQLIMDECKTFFFAGHETTALLLTWTTMLLAD 361

Query: 363 SPDWQRILREEIKEVVGDKDI-DFSMLSSLKKMGWVWNEVLRLYPSAPNIQRQAKGDIDL 422
           +P WQ  +REE++EV G   +     LS L  +  V NE LRLYP A  + R A  D+ L
Sbjct: 362 NPTWQEKVREEVREVFGRNGLPSVDQLSKLTSLSKVINESLRLYPPATLLPRMAFEDLKL 421

Query: 423 GDIKIPKGTNIWIDVVAMHHDPTLWGDDVNDFNPSRFQHDTIHGGCNHKMG--YLPFGFG 482
           GD+ IPKG +IWI V+A+HH   LWG D N FNP RF      GG     G  ++PF  G
Sbjct: 422 GDLTIPKGLSIWIPVLAIHHSEELWGKDANQFNPERF------GGRPFASGRHFIPFAAG 481

Query: 483 GRMCIGRNLSSMEYKIVLTLILSTFSLSLSPSYSHSPATLLSLRPAHGIPLILTPL 524
            R CIG+  + ME KI+L  ++S F+ ++S +Y H+P  +L+++P +G+ +IL PL
Sbjct: 482 PRNCIGQQFALMEAKIILATLISKFNFTISKNYRHAPIVVLTIKPKYGVQVILKPL 516

BLAST of Cmc06g0158781 vs. ExPASy Swiss-Prot
Match: B6SSW8 (Cytochrome P450 714B3 OS=Zea mays OX=4577 GN=CYP714B3 PE=2 SV=1)

HSP 1 Score: 307.8 bits (787), Expect = 2.4e-82
Identity = 179/503 (35.59%), Postives = 270/503 (53.68%), Query Frame = 0

Query: 31  WILPILAHRKLKRNGFEGPSPSFPLGNITEMKKIMKA--AAAATAVTNGSSSNNLSHDIH 90
           W++P     K +     GP PSFP GN+ +M++   A  AA A+A  +GS    + HD  
Sbjct: 32  WLVPQKVLAKFEDQKIGGPRPSFPYGNLADMREAAAAAKAARASARRSGSGGGGIVHDYR 91

Query: 91  STIFPHFAQWQNSYGKKFVYWLGTEPFLYIADPQLVKKISEAVLGKSWGKPAVFRNDREP 150
             + P++ +W+  YG  F Y +G   FL+++ P +V+ I+  V     GK +  +   EP
Sbjct: 92  PAVLPYYEKWRKEYGPIFTYSMGNVVFLHVSRPDVVRDINLCV-SLDLGKSSYLKATHEP 151

Query: 151 MFGDGLVMTEGDNWIRHRHILTPAFNPTNLKAMTKFMVESTTKMLEEWRSLV--KSGQSE 210
           +FG G++ + G+ W+  R I+ P F    +K M   MV+S   +L  W   V    G ++
Sbjct: 152 LFGGGILKSNGEAWLHQRKIIAPEFFLDKVKGMVDLMVDSAQPLLMSWEERVDRNGGITD 211

Query: 211 IEVERQITATAGEIIAKASFGISNCGGGGEALNNLRALQLTLFKNSSYVGVPFSSLFLYP 270
           I+++  I A + ++I++  FG S+   G E    +R LQ  + K +  V    + L  +P
Sbjct: 212 IKIDDDIRAYSADVISRTCFG-SSYIKGKEIFMKIRELQQAVSKPN--VLAEMTGLRFFP 271

Query: 271 A-RTLAAKRLGAQIDRLFLSIISNRKMSGSGSGYSDLLSRLIGSEYTGGEALSPRE--VV 330
           + R   A  L  Q+ +L L I+   K SG      +LLS ++ S  T    ++  E  +V
Sbjct: 272 SMRNKQAWELHKQVRKLILEIV---KESGED---RNLLSAILHSASTSRVGIAEAENFIV 331

Query: 331 DECKTFFFGGHETTALAISWTLLLLATSPDWQRILREEIKEVVGDKDIDFSMLSSLKKMG 390
           D CK+ +F GHE+TA+  +W L+LL   P+WQ  +REE+ EV   + +D   L  +K + 
Sbjct: 332 DNCKSIYFAGHESTAVTAAWCLMLLGLHPEWQNRVREEVHEVCRGQPVDSRSLQKMKNLT 391

Query: 391 WVWNEVLRLYPSAPNIQRQAKGDIDLGDIKIPKGTNIWIDVVAMHHDPTLWGDDVNDFNP 450
            V  E LRLYP+   + RQA  ++ LG + IPKG NI+I V  MH DP LWG DV +FNP
Sbjct: 392 MVIQETLRLYPAGAFVSRQALQELKLGGVHIPKGVNIYIPVSTMHLDPELWGPDVKEFNP 451

Query: 451 SRFQ--HDTIHGGCNHKMGYLPFGFGGRMCIGRNLSSMEYKIVLTLILSTFSLSLSPSYS 510
            RF      +H        YLPFG G R C+G+  +  E KI+++LI+S F L LSP Y 
Sbjct: 452 ERFSDVRPQLH-------SYLPFGAGARTCLGQGFAMAELKILISLIVSKFVLKLSPHYQ 511

Query: 511 HSPATLLSLRPAHGIPLILTPLQ 525
           HSP   L + P  G+ L LT +Q
Sbjct: 512 HSPTLKLIVEPELGVDLTLTKVQ 517

BLAST of Cmc06g0158781 vs. ExPASy Swiss-Prot
Match: Q6NKZ8 (Cytochrome P450 714A2 OS=Arabidopsis thaliana OX=3702 GN=CYP714A2 PE=2 SV=1)

HSP 1 Score: 303.5 bits (776), Expect = 4.6e-81
Identity = 169/510 (33.14%), Postives = 269/510 (52.75%), Query Frame = 0

Query: 22  LFGKVVISCWILPILAHRKLKRNGFEGPSPSFPLGNITEMKKIMKAAAAATAVTNGSSSN 81
           ++G+ V+  W +     R LK  G +GP PS   GN++EM++I   A       + S  N
Sbjct: 27  VYGRAVVEQWRM----RRSLKLQGVKGPPPSIFNGNVSEMQRIQSEA------KHCSGDN 86

Query: 82  NLSHDIHSTIFPHFAQWQNSYGKKFVYWLGTEPFLYIADPQLVKKISEAVLGKSWGKPAV 141
            +SHD  S++FPHF  W+  YG+ + Y  G +  LYI  P++VK++S+     + G+   
Sbjct: 87  IISHDYSSSLFPHFDHWRKQYGRIYTYSTGLKQHLYINHPEMVKELSQTNT-LNLGRITH 146

Query: 142 FRNDREPMFGDGLVMTEGDNWIRHRHILTPAFNPTNLKAMTKFMVESTTKMLEEWRSLVK 201
                 P+ G+G++ + G +W   R I+   F    +K M   MVES   ML +W  +VK
Sbjct: 147 ITKRLNPILGNGIITSNGPHWAHQRRIIAYEFTHDKIKGMVGLMVESAMPMLNKWEEMVK 206

Query: 202 SGQS---EIEVERQITATAGEIIAKASFGISNCGGGGEALNNLRAL------QLTLFKNS 261
            G     +I V+  +   + ++IAKA FG S+   G    + +R L      +  LF+ +
Sbjct: 207 RGGEMGCDIRVDEDLKDVSADVIAKACFG-SSFSKGKAIFSMIRDLLTAITKRSVLFRFN 266

Query: 262 SYVGVPFSSLFLYPARTLAAKRLGAQIDRLFLSIISNRKMSGSGSGYSDLLSRLIGSEYT 321
            +  + F S        +    L  +++      +  R++    +   DL+  ++     
Sbjct: 267 GFTDMVFGS---KKHGDVDIDALEMELESSIWETVKEREIECKDTHKKDLMQLILEGAMR 326

Query: 322 G------GEALSPREVVDECKTFFFGGHETTALAISWTLLLLATSPDWQRILREEIKEVV 381
                   ++   R VVD CK+ +F GH++TA+++SW L+LLA +P WQ  +R+EI    
Sbjct: 327 SCDGNLWDKSAYRRFVVDNCKSIYFAGHDSTAVSVSWCLMLLALNPSWQVKIRDEILSSC 386

Query: 382 GDKDIDFSMLSSLKKMGWVWNEVLRLYPSAPNIQRQAKGDIDLGDIKIPKGTNIWIDVVA 441
            +   D   + +LK +  V  E +RLYP AP + R+A  DI LGD+ +PKG  IW  + A
Sbjct: 387 KNGIPDAESIPNLKTVTMVIQETMRLYPPAPIVGREASKDIRLGDLVVPKGVCIWTLIPA 446

Query: 442 MHHDPTLWGDDVNDFNPSRFQHDTIHGGCNHKMGYLPFGFGGRMCIGRNLSSMEYKIVLT 501
           +H DP +WG D NDF P RF  + I   C +   Y+PFG G R C+G+N   ME K++++
Sbjct: 447 LHRDPEIWGPDANDFKPERFS-EGISKACKYPQSYIPFGLGPRTCVGKNFGMMEVKVLVS 506

Query: 502 LILSTFSLSLSPSYSHSPATLLSLRPAHGI 517
           LI+S FS +LSP+Y HSP+  L + P HG+
Sbjct: 507 LIVSKFSFTLSPTYQHSPSHKLLVEPQHGV 520

BLAST of Cmc06g0158781 vs. ExPASy TrEMBL
Match: A0A1S3AXX2 (cytokinin hydroxylase OS=Cucumis melo OX=3656 GN=LOC103484084 PE=3 SV=1)

HSP 1 Score: 1051.6 bits (2718), Expect = 1.1e-303
Identity = 524/525 (99.81%), Postives = 524/525 (99.81%), Query Frame = 0

Query: 1   MELICVLIMLIKWMIRVSIMVLFGKVVISCWILPILAHRKLKRNGFEGPSPSFPLGNITE 60
           MELICVLIMLIKWMIRVSIMVLFGKVVISCWILPILAHRKLKRNGFEGPSPSFPLGNITE
Sbjct: 1   MELICVLIMLIKWMIRVSIMVLFGKVVISCWILPILAHRKLKRNGFEGPSPSFPLGNITE 60

Query: 61  MKKIMKAAAAATAVTNGSSSNNLSHDIHSTIFPHFAQWQNSYGKKFVYWLGTEPFLYIAD 120
           MKKIMKAAAAATAVTNGSSSNNLSHDIHSTIFPHFAQWQNSYGKKFVYWLGTEPFLYIAD
Sbjct: 61  MKKIMKAAAAATAVTNGSSSNNLSHDIHSTIFPHFAQWQNSYGKKFVYWLGTEPFLYIAD 120

Query: 121 PQLVKKISEAVLGKSWGKPAVFRNDREPMFGDGLVMTEGDNWIRHRHILTPAFNPTNLKA 180
           PQLVKKISEAVLGKSWGKPAVFRNDREPMFGDGLVMTEGDNWIRHRHILTPAFNPTNLKA
Sbjct: 121 PQLVKKISEAVLGKSWGKPAVFRNDREPMFGDGLVMTEGDNWIRHRHILTPAFNPTNLKA 180

Query: 181 MTKFMVESTTKMLEEWRSLVKSGQSEIEVERQITATAGEIIAKASFGISNCGGGGEALNN 240
           MTKFMVESTTKMLEEWRSLVKSGQSEIEVERQITATAGEIIAKASFGISNCGGGGEALNN
Sbjct: 181 MTKFMVESTTKMLEEWRSLVKSGQSEIEVERQITATAGEIIAKASFGISNCGGGGEALNN 240

Query: 241 LRALQLTLFKNSSYVGVPFSSLFLYPARTLAAKRLGAQIDRLFLSIISNRKMSGSGSGYS 300
           LRALQLTLFKNSSYVGVPFSSLFLYPARTLAAKRLGAQIDRLFLSIISNRKMSGSGSGYS
Sbjct: 241 LRALQLTLFKNSSYVGVPFSSLFLYPARTLAAKRLGAQIDRLFLSIISNRKMSGSGSGYS 300

Query: 301 DLLSRLIGSEYTGGEALSPREVVDECKTFFFGGHETTALAISWTLLLLATSPDWQRILRE 360
           DLLSRLIGSEYTGGEALSPREVVDECKTFFFGGHETTALAISWTLLLLATSPDWQRILRE
Sbjct: 301 DLLSRLIGSEYTGGEALSPREVVDECKTFFFGGHETTALAISWTLLLLATSPDWQRILRE 360

Query: 361 EIKEVVGDKDIDFSMLSSLKKMGWVWNEVLRLYPSAPNIQRQAKGDIDLGDIKIPKGTNI 420
           EIKEVVGDKDIDFSMLSSLKKMGWVWNEVLRLYPSAPNIQRQAKGDIDLGDIKIPKGTNI
Sbjct: 361 EIKEVVGDKDIDFSMLSSLKKMGWVWNEVLRLYPSAPNIQRQAKGDIDLGDIKIPKGTNI 420

Query: 421 WIDVVAMHHDPTLWGDDVNDFNPSRFQHDTIHGGCNHKMGYLPFGFGGRMCIGRNLSSME 480
           WIDVVAMHHDPTLWGDDVNDFNPSRFQHDTIHGGC HKMGYLPFGFGGRMCIGRNLSSME
Sbjct: 421 WIDVVAMHHDPTLWGDDVNDFNPSRFQHDTIHGGCYHKMGYLPFGFGGRMCIGRNLSSME 480

Query: 481 YKIVLTLILSTFSLSLSPSYSHSPATLLSLRPAHGIPLILTPLQP 526
           YKIVLTLILSTFSLSLSPSYSHSPATLLSLRPAHGIPLILTPLQP
Sbjct: 481 YKIVLTLILSTFSLSLSPSYSHSPATLLSLRPAHGIPLILTPLQP 525

BLAST of Cmc06g0158781 vs. ExPASy TrEMBL
Match: A0A5D3DI76 (Cytokinin hydroxylase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold386G00640 PE=3 SV=1)

HSP 1 Score: 941.8 bits (2433), Expect = 1.2e-270
Identity = 465/465 (100.00%), Postives = 465/465 (100.00%), Query Frame = 0

Query: 61  MKKIMKAAAAATAVTNGSSSNNLSHDIHSTIFPHFAQWQNSYGKKFVYWLGTEPFLYIAD 120
           MKKIMKAAAAATAVTNGSSSNNLSHDIHSTIFPHFAQWQNSYGKKFVYWLGTEPFLYIAD
Sbjct: 1   MKKIMKAAAAATAVTNGSSSNNLSHDIHSTIFPHFAQWQNSYGKKFVYWLGTEPFLYIAD 60

Query: 121 PQLVKKISEAVLGKSWGKPAVFRNDREPMFGDGLVMTEGDNWIRHRHILTPAFNPTNLKA 180
           PQLVKKISEAVLGKSWGKPAVFRNDREPMFGDGLVMTEGDNWIRHRHILTPAFNPTNLKA
Sbjct: 61  PQLVKKISEAVLGKSWGKPAVFRNDREPMFGDGLVMTEGDNWIRHRHILTPAFNPTNLKA 120

Query: 181 MTKFMVESTTKMLEEWRSLVKSGQSEIEVERQITATAGEIIAKASFGISNCGGGGEALNN 240
           MTKFMVESTTKMLEEWRSLVKSGQSEIEVERQITATAGEIIAKASFGISNCGGGGEALNN
Sbjct: 121 MTKFMVESTTKMLEEWRSLVKSGQSEIEVERQITATAGEIIAKASFGISNCGGGGEALNN 180

Query: 241 LRALQLTLFKNSSYVGVPFSSLFLYPARTLAAKRLGAQIDRLFLSIISNRKMSGSGSGYS 300
           LRALQLTLFKNSSYVGVPFSSLFLYPARTLAAKRLGAQIDRLFLSIISNRKMSGSGSGYS
Sbjct: 181 LRALQLTLFKNSSYVGVPFSSLFLYPARTLAAKRLGAQIDRLFLSIISNRKMSGSGSGYS 240

Query: 301 DLLSRLIGSEYTGGEALSPREVVDECKTFFFGGHETTALAISWTLLLLATSPDWQRILRE 360
           DLLSRLIGSEYTGGEALSPREVVDECKTFFFGGHETTALAISWTLLLLATSPDWQRILRE
Sbjct: 241 DLLSRLIGSEYTGGEALSPREVVDECKTFFFGGHETTALAISWTLLLLATSPDWQRILRE 300

Query: 361 EIKEVVGDKDIDFSMLSSLKKMGWVWNEVLRLYPSAPNIQRQAKGDIDLGDIKIPKGTNI 420
           EIKEVVGDKDIDFSMLSSLKKMGWVWNEVLRLYPSAPNIQRQAKGDIDLGDIKIPKGTNI
Sbjct: 301 EIKEVVGDKDIDFSMLSSLKKMGWVWNEVLRLYPSAPNIQRQAKGDIDLGDIKIPKGTNI 360

Query: 421 WIDVVAMHHDPTLWGDDVNDFNPSRFQHDTIHGGCNHKMGYLPFGFGGRMCIGRNLSSME 480
           WIDVVAMHHDPTLWGDDVNDFNPSRFQHDTIHGGCNHKMGYLPFGFGGRMCIGRNLSSME
Sbjct: 361 WIDVVAMHHDPTLWGDDVNDFNPSRFQHDTIHGGCNHKMGYLPFGFGGRMCIGRNLSSME 420

Query: 481 YKIVLTLILSTFSLSLSPSYSHSPATLLSLRPAHGIPLILTPLQP 526
           YKIVLTLILSTFSLSLSPSYSHSPATLLSLRPAHGIPLILTPLQP
Sbjct: 421 YKIVLTLILSTFSLSLSPSYSHSPATLLSLRPAHGIPLILTPLQP 465

BLAST of Cmc06g0158781 vs. ExPASy TrEMBL
Match: A0A5A7SWW2 (Cytokinin hydroxylase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold239G001260 PE=3 SV=1)

HSP 1 Score: 938.7 bits (2425), Expect = 1.0e-269
Identity = 464/465 (99.78%), Postives = 464/465 (99.78%), Query Frame = 0

Query: 61  MKKIMKAAAAATAVTNGSSSNNLSHDIHSTIFPHFAQWQNSYGKKFVYWLGTEPFLYIAD 120
           MKKIMKAAAAATAVTNGSSSNNLSHDIHSTIFPHFAQWQNSYGKKFVYWLGTEPFLYIAD
Sbjct: 1   MKKIMKAAAAATAVTNGSSSNNLSHDIHSTIFPHFAQWQNSYGKKFVYWLGTEPFLYIAD 60

Query: 121 PQLVKKISEAVLGKSWGKPAVFRNDREPMFGDGLVMTEGDNWIRHRHILTPAFNPTNLKA 180
           PQLVKKISEAVLGKSWGKPAVFRNDREPMFGDGLVMTEGDNWIRHRHILTPAFNPTNLKA
Sbjct: 61  PQLVKKISEAVLGKSWGKPAVFRNDREPMFGDGLVMTEGDNWIRHRHILTPAFNPTNLKA 120

Query: 181 MTKFMVESTTKMLEEWRSLVKSGQSEIEVERQITATAGEIIAKASFGISNCGGGGEALNN 240
           MTKFMVESTTKMLEEWRSLVKSGQSEIEVERQITATAGEIIAKASFGISNCGGGGEALNN
Sbjct: 121 MTKFMVESTTKMLEEWRSLVKSGQSEIEVERQITATAGEIIAKASFGISNCGGGGEALNN 180

Query: 241 LRALQLTLFKNSSYVGVPFSSLFLYPARTLAAKRLGAQIDRLFLSIISNRKMSGSGSGYS 300
           LRALQLTLFKNSSYVGVPFSSLFLYPARTLAAKRLGAQIDRLFLSIISNRKMSGSGSGYS
Sbjct: 181 LRALQLTLFKNSSYVGVPFSSLFLYPARTLAAKRLGAQIDRLFLSIISNRKMSGSGSGYS 240

Query: 301 DLLSRLIGSEYTGGEALSPREVVDECKTFFFGGHETTALAISWTLLLLATSPDWQRILRE 360
           DLLSRLIGSEYTGGEALSPREVVDECKTFFFGGHETTALAISWTLLLLATSPDWQRILRE
Sbjct: 241 DLLSRLIGSEYTGGEALSPREVVDECKTFFFGGHETTALAISWTLLLLATSPDWQRILRE 300

Query: 361 EIKEVVGDKDIDFSMLSSLKKMGWVWNEVLRLYPSAPNIQRQAKGDIDLGDIKIPKGTNI 420
           EIKEVVGDKDIDFSMLSSLKKMGWVWNEVLRLYPSAPNIQRQAKGDIDLGDIKIPKGTNI
Sbjct: 301 EIKEVVGDKDIDFSMLSSLKKMGWVWNEVLRLYPSAPNIQRQAKGDIDLGDIKIPKGTNI 360

Query: 421 WIDVVAMHHDPTLWGDDVNDFNPSRFQHDTIHGGCNHKMGYLPFGFGGRMCIGRNLSSME 480
           WIDVVAMHHDPTLWGDDVNDFNPSRFQHDTIHGGC HKMGYLPFGFGGRMCIGRNLSSME
Sbjct: 361 WIDVVAMHHDPTLWGDDVNDFNPSRFQHDTIHGGCYHKMGYLPFGFGGRMCIGRNLSSME 420

Query: 481 YKIVLTLILSTFSLSLSPSYSHSPATLLSLRPAHGIPLILTPLQP 526
           YKIVLTLILSTFSLSLSPSYSHSPATLLSLRPAHGIPLILTPLQP
Sbjct: 421 YKIVLTLILSTFSLSLSPSYSHSPATLLSLRPAHGIPLILTPLQP 465

BLAST of Cmc06g0158781 vs. ExPASy TrEMBL
Match: A0A6J1CIQ8 (cytokinin hydroxylase OS=Momordica charantia OX=3673 GN=LOC111011538 PE=3 SV=1)

HSP 1 Score: 797.3 bits (2058), Expect = 3.7e-227
Identity = 404/533 (75.80%), Postives = 452/533 (84.80%), Query Frame = 0

Query: 8   IMLIKW-MIRVSIMVLFGKVVISCWILPILAHRKLKRNGFE-GPSPSFPLGNITEMKKIM 67
           I +I W +IRVS++VL  +VV S WILP+LAHRKLKRNGF  GP P FPLGNITE+K  M
Sbjct: 4   IGVIVWGIIRVSLVVLLVRVVFSWWILPVLAHRKLKRNGFNGGPPPRFPLGNITEIKNTM 63

Query: 68  K--AAAAATAVTNG-----SSSNNLSHDIHSTIFPHFAQWQNSYGKKFVYWLGTEPFLYI 127
           K  AA+AA A  NG     SSS+N+SHDIHST+FP+FAQWQ SYGKKFVYWLGTEPFLYI
Sbjct: 64  KKAAASAAAAAINGGAGAFSSSSNISHDIHSTVFPYFAQWQTSYGKKFVYWLGTEPFLYI 123

Query: 128 ADPQLVKKISEAVLGKSWGKPAVFRNDREPMFGDGLVMTEGDNWIRHRHILTPAFNPTNL 187
           A+PQ VK +SEAV+GK WGKPAVFRNDREPMFGDGLVMTEGD+WIRHRHILTPAFNP NL
Sbjct: 124 AEPQFVKNMSEAVMGKGWGKPAVFRNDREPMFGDGLVMTEGDDWIRHRHILTPAFNPANL 183

Query: 188 KAMTKFMVESTTKMLEEWRSLVKSGQSEIEVERQITATAGEIIAKASFGISNCGGGGEAL 247
           KAMT+ MV STTKML+EW   + SG  E++VER+ITATAG+IIAKASFGI +   G +A 
Sbjct: 184 KAMTRLMVGSTTKMLDEWARRLSSGGPEMDVEREITATAGDIIAKASFGICD-RSGRDAF 243

Query: 248 NNLRALQLTLFKNSSYVGVPFSSLFLYPARTLAAKRLGAQIDRLFLSIISNRKMSGSGSG 307
           N LR LQ TLF+NSSYVGVPFSS+FLYP RTL AKRLG++IDRLFLSIIS RK S   S 
Sbjct: 244 NKLRDLQFTLFRNSSYVGVPFSSIFLYPTRTLTAKRLGSEIDRLFLSIISERKQSPDASA 303

Query: 308 YSDLLSRLIGSE------YTGGEALSPREVVDECKTFFFGGHETTALAISWTLLLLATSP 367
            SDLLSRLI S+        G   L+ RE+VDECKTFFFGGHETTALAISWT+LLLAT P
Sbjct: 304 -SDLLSRLIESQRNDSAPAAGKSWLTTRELVDECKTFFFGGHETTALAISWTMLLLATRP 363

Query: 368 DWQRILREEIKEVVGDKDIDFSMLSSLKKMGWVWNEVLRLYPSAPNIQRQAKGDIDLGDI 427
           +WQ ILREEIKEV+GD++IDF+MLS LKKMGWV+NEVLRLYPSAPNIQRQAKGDI +GD 
Sbjct: 364 EWQTILREEIKEVIGDREIDFAMLSRLKKMGWVFNEVLRLYPSAPNIQRQAKGDIKVGDT 423

Query: 428 KIPKGTNIWIDVVAMHHDPTLWGDDVNDFNPSRFQHDTIHGGCNHKMGYLPFGFGGRMCI 487
            IPKGTNIWID+VAMHHDP+LWGDDVN+F P RF+ D+IHGGC HKMGYLPFGFGGRMCI
Sbjct: 424 TIPKGTNIWIDIVAMHHDPSLWGDDVNEFKPERFEKDSIHGGCKHKMGYLPFGFGGRMCI 483

Query: 488 GRNLSSMEYKIVLTLILSTFSLSLSPSYSHSPATLLSLRPAHGIPLILTPLQP 526
           GRNLSSMEYKIVLTLI+S FSLS+SPSYSHSPA LLSLRPAHG+PLIL PL P
Sbjct: 484 GRNLSSMEYKIVLTLIISRFSLSISPSYSHSPAILLSLRPAHGLPLILKPLHP 534

BLAST of Cmc06g0158781 vs. ExPASy TrEMBL
Match: A0A6J1HYD5 (cytochrome P450 714C2-like OS=Cucurbita maxima OX=3661 GN=LOC111467387 PE=3 SV=1)

HSP 1 Score: 790.8 bits (2041), Expect = 3.5e-225
Identity = 397/529 (75.05%), Postives = 449/529 (84.88%), Query Frame = 0

Query: 1   MELICVLIMLIKWMIRVSIMVLFGKVVISCWILPILAHRKLKRNGFEGPSPSFPLGNITE 60
           M  I + I LI W++   IMVLF KVV+S W+LP+LAH+KLKRNGF GP P+FPLGNI E
Sbjct: 1   MVAISLSIGLITWVL---IMVLFVKVVVSWWVLPVLAHQKLKRNGFRGPPPTFPLGNIAE 60

Query: 61  MKKIMKAAAAATAVTNGSSSNNLSHDIHSTIFPHFAQWQNSYGKKFVYWLGTEPFLYIAD 120
           MK+I KA+AA    ++   S+ +SHDIHST+FPHFAQWQ SYGKKFVYWLGTEPFLYIAD
Sbjct: 61  MKRIRKASAAINGDSHSFPSSKVSHDIHSTVFPHFAQWQASYGKKFVYWLGTEPFLYIAD 120

Query: 121 PQLVKKISEAVLGKSWGKPAVFRNDREPMFGDGLVMTEGDNWIRHRHILTPAFNPTNLKA 180
           P  +K+IS AV+ K WGKPAVFRNDR PMFGDGLVMTEGD W+RHRHILTPAFNP NLKA
Sbjct: 121 PHFMKQISVAVMAKGWGKPAVFRNDRRPMFGDGLVMTEGDVWVRHRHILTPAFNPANLKA 180

Query: 181 MTKFMVESTTKMLEEWRSLVKSGQSEIEVERQITATAGEIIAKASFGISNCGGGGEALNN 240
           MTKFMVESTTKML EW SLV S   EI+VER+ITATAG+IIAKASFGI N GGG +A + 
Sbjct: 181 MTKFMVESTTKMLNEWSSLVNSASGEIDVEREITATAGDIIAKASFGICN-GGGRDAFDY 240

Query: 241 LRALQLTLFKNSSYVGVPFSSLFLYPARTLAAKRLGAQIDRLFLSIISNRKMSGSGSGYS 300
           LRALQ TLF+NS+YVGVPFSSLF  P RTLAAKRLGA IDRLFLS++S RK+S + S  S
Sbjct: 241 LRALQFTLFQNSTYVGVPFSSLFFCPGRTLAAKRLGAAIDRLFLSLVSERKLSQNSS--S 300

Query: 301 DLLSRLIGSEYTGGE----ALSPREVVDECKTFFFGGHETTALAISWTLLLLATSPDWQR 360
           DLLSRLI  + + G+     L+ RE+VDECKTFFFGGHETTALA SWTLLLLAT P+WQ 
Sbjct: 301 DLLSRLIEGQKSTGDVGKMGLTTRELVDECKTFFFGGHETTALATSWTLLLLATRPEWQD 360

Query: 361 ILREEIKEVVGDKDIDFSMLSSLKKMGWVWNEVLRLYPSAPNIQRQAKGDIDLGDIKIPK 420
           ILREEIKEV+GDK+IDF+MLS LKKMG V++EVLRLYPSAPNIQRQAKGDI++G + IPK
Sbjct: 361 ILREEIKEVIGDKEIDFNMLSGLKKMGCVFSEVLRLYPSAPNIQRQAKGDIEVGSMTIPK 420

Query: 421 GTNIWIDVVAMHHDPTLWGDDVNDFNPSRFQHDTIHGGCNHKMGYLPFGFGGRMCIGRNL 480
           GTNIWID+VAMH DP LWGDD N+F P RF  D+I GGCNHKMGYLPFGFGGRMCIG++L
Sbjct: 421 GTNIWIDIVAMHRDPDLWGDDANEFKPERFVEDSIRGGCNHKMGYLPFGFGGRMCIGKSL 480

Query: 481 SSMEYKIVLTLILSTFSLSLSPSYSHSPATLLSLRPAHGIPLILTPLQP 526
           SSMEYKIVLTLILS FSLSLSP+YSHSPATLLSLRPAHG+PLI+TPL P
Sbjct: 481 SSMEYKIVLTLILSRFSLSLSPNYSHSPATLLSLRPAHGLPLIVTPLHP 523

BLAST of Cmc06g0158781 vs. TAIR 10
Match: AT5G52400.1 (cytochrome P450, family 715, subfamily A, polypeptide 1 )

HSP 1 Score: 611.7 bits (1576), Expect = 5.6e-175
Identity = 297/510 (58.24%), Postives = 375/510 (73.53%), Query Frame = 0

Query: 17  VSIMVLFGKVVISCWILPILAHRKLKRNGFEGPSPSFPLGNITEMKKIMKAAAAATAVTN 76
           V I ++F K+ + CWI P+ A +KL+ NGF GP+PSFP GN+ +MKK+     A+  V N
Sbjct: 19  VVICLMFLKLFLRCWIWPVRAQKKLRGNGFVGPAPSFPFGNLNDMKKL---KMASVVVDN 78

Query: 77  GSSSNNLSHDIHSTIFPHFAQWQNSYGKKFVYWLGTEPFLYIADPQLVKKISEAVLGKSW 136
             SS  ++HDIHS   PHFA+WQ  YGK FVYWLG EPF+Y+ADP+ +  +S+ VLGKSW
Sbjct: 79  SKSSTIINHDIHSIALPHFARWQQEYGKVFVYWLGIEPFVYVADPEFLSVMSKGVLGKSW 138

Query: 137 GKPAVFRNDREPMFGDGLVMTEGDNWIRHRHILTPAFNPTNLKAMTKFMVESTTKMLEEW 196
           GKP VF+ DREPMFG GLVM EGD+W RHRHI+TPAF P NLK MT  MVES + ML+ W
Sbjct: 139 GKPNVFKKDREPMFGTGLVMVEGDDWTRHRHIITPAFAPLNLKVMTNMMVESVSNMLDRW 198

Query: 197 RSLVKSGQSEIEVERQITATAGEIIAKASFGISNCGGGGEALNNLRALQLTLFKNSSYVG 256
              + SG  E ++E +I  TAGEIIAK SFG++    G + L NLRA+Q  LF ++ YVG
Sbjct: 199 GIQINSGNPEFDMESEIIGTAGEIIAKTSFGVTG-ENGTQVLKNLRAVQFALFNSNRYVG 258

Query: 257 VPFSSLFLYPARTLAAKRLGAQIDRLFLSIISNRKMS---GSGSGYSDLLSRLIGSEYTG 316
           VPFS++  Y  +T+ AK LG +ID L LS I+ RK+S   G   G+ DLL  L+ ++  G
Sbjct: 259 VPFSNILSY-KQTVKAKGLGHEIDGLLLSFINKRKISLAEGDDQGH-DLLGMLLKADQKG 318

Query: 317 GEALSPREVVDECKTFFFGGHETTALAISWTLLLLATSPDWQRILREEIKEVVGDKDIDF 376
               + +E+VDECKTFFF GHETTALA++WT +LLA  P+WQ  +REEI+EV+GD  I++
Sbjct: 319 N--FTAKELVDECKTFFFAGHETTALALTWTFMLLAIHPEWQDTIREEIREVIGDSKIEY 378

Query: 377 SMLSSLKKMGWVWNEVLRLYPSAPNIQRQAKGDIDLGDIKIPKGTNIWIDVVAMHHDPTL 436
           + L+ LKKM WV NEVLRLYP APN QRQA+ DI++    IP GTNIWIDVVAMHHD  L
Sbjct: 379 NKLAGLKKMSWVMNEVLRLYPPAPNAQRQARNDIEVNGRVIPNGTNIWIDVVAMHHDVEL 438

Query: 437 WGDDVNDFNPSRFQHDTIHGGCNHKMGYLPFGFGGRMCIGRNLSSMEYKIVLTLILSTFS 496
           WGDDVN+F P RF    +HGGC +KMGY+PFGFGGRMCIGRNL++MEYKIVL+L+LS F 
Sbjct: 439 WGDDVNEFKPERFD-GNLHGGCKNKMGYMPFGFGGRMCIGRNLTTMEYKIVLSLVLSRFE 498

Query: 497 LSLSPSYSHSPATLLSLRPAHGIPLILTPL 524
           +S+SP Y HSP  +LSLRP +G+PLI+ PL
Sbjct: 499 ISVSPGYRHSPTYMLSLRPGYGLPLIIRPL 519

BLAST of Cmc06g0158781 vs. TAIR 10
Match: AT1G67110.1 (cytochrome P450, family 735, subfamily A, polypeptide 2 )

HSP 1 Score: 322.0 bits (824), Expect = 8.8e-88
Identity = 191/530 (36.04%), Postives = 294/530 (55.47%), Query Frame = 0

Query: 6   VLIMLIKWMIRVSIMVLFGKVV---ISCWIL-PILAHRKLKRNGFEGPSPSFPLGNITEM 65
           +++ L+   + V +M L  +V+   I C+ L P    + ++R G  GP P    GNI ++
Sbjct: 1   MMVTLVLKYVLVIVMTLILRVLYDSICCYFLTPRRIKKFMERQGITGPKPRLLTGNIIDI 60

Query: 66  KKIMKAAAAATAVTNGSSSNNLSHDIHSTIFPHFAQWQNSYGKKFVYWLGTEPFLYIADP 125
            K++  +A+       +  +++ H+I   + PH+  W   YGK+F+ W GTEP L + + 
Sbjct: 61  SKMLSHSAS-------NDCSSIHHNIVPRLLPHYVSWSKQYGKRFIMWNGTEPRLCLTET 120

Query: 126 QLVKKI---SEAVLGKSWGKPAVFRNDREPMFGDGLVMTEGDNWIRHRHILTPAFNPTNL 185
           +++K++      V GKSW    + +   +   G GL+M  G+ W   RH+  PAF    L
Sbjct: 121 EMIKELLTKHNPVTGKSW----LQQQGTKGFIGRGLLMANGEAWHHQRHMAAPAFTRDRL 180

Query: 186 KAMTKFMVESTTKMLEEWRSLVKSGQSEIEVERQITATAGEIIAKASFGISNCGGGGEAL 245
           K   K MVE T  M E  R   K    E+E+  ++     +II++  FG S+C  G E  
Sbjct: 181 KGYAKHMVECTKMMAERLR---KEVGEEVEIGEEMRRLTADIISRTEFG-SSCDKGKELF 240

Query: 246 NNLRALQLTLFKNSSYVGVPFSSLFLYPARTLAAKRLGAQIDRLFLSIISNRKMS---GS 305
           + L  LQ    + + ++  P  S FL        K L  +++RL + II +RK S   G 
Sbjct: 241 SLLTVLQRLCAQATRHLCFP-GSRFLPSKYNREIKSLKTEVERLLMEIIDSRKDSVEIGR 300

Query: 306 GSGY-SDLLSRLIGSEYTGGEALSPREVVDECKTFFFGGHETTALAISWTLLLLATSPDW 365
            S Y  DLL  L+    +    L+ + ++DECKTFFF GHETT+L ++WTL+LLA +P W
Sbjct: 301 SSSYGDDLLGLLLNQMDSNKNNLNVQMIMDECKTFFFTGHETTSLLLTWTLMLLAHNPTW 360

Query: 366 QRILREEIKEVVGDKDI-DFSMLSSLKKMGWVWNEVLRLYPSAPNIQRQAKGDIDLGDIK 425
           Q  +R+E+++V G   +     LSSL  +  V NE LRLYP A  + R A  DI LGD+ 
Sbjct: 361 QDNVRDEVRQVCGQDGVPSVEQLSSLTSLNKVINESLRLYPPATLLPRMAFEDIKLGDLI 420

Query: 426 IPKGTNIWIDVVAMHHDPTLWGDDVNDFNPSRFQHDTIHGGCNHKMGYLPFGFGGRMCIG 485
           IPKG +IWI V+A+HH   LWG+D N+FNP RF   +      H   ++PF  G R CIG
Sbjct: 421 IPKGLSIWIPVLAIHHSNELWGEDANEFNPERFTTRSF-ASSRH---FMPFAAGPRNCIG 480

Query: 486 RNLSSMEYKIVLTLILSTFSLSLSPSYSHSPATLLSLRPAHGIPLILTPL 524
           +  + ME KI+L +++S FS ++S +Y H+P  +L+++P +G+ L+L PL
Sbjct: 481 QTFAMMEAKIILAMLVSKFSFAISENYRHAPIVVLTIKPKYGVQLVLKPL 510

BLAST of Cmc06g0158781 vs. TAIR 10
Match: AT5G38450.1 (cytochrome P450, family 735, subfamily A, polypeptide 1 )

HSP 1 Score: 315.5 bits (807), Expect = 8.3e-86
Identity = 189/536 (35.26%), Postives = 290/536 (54.10%), Query Frame = 0

Query: 3   LICVLIMLIKWMIRVSIMVLFGKVVISC-WILPILAHRKLKRNGFEGPSPSFPLGNITEM 62
           L+ +L  L+   +   + VL+    ISC W+ P    + +++ G  GP P    GNI E+
Sbjct: 2   LLTILKSLLVIFVTTILRVLYD--TISCYWLTPRRIKKIMEQQGVTGPKPRPLTGNILEI 61

Query: 63  KKIMKAAAAATAVTNGSSSNNLSHDIHSTIFPHFAQWQNSYGKKFVYWLGTEPFLYIADP 122
             ++  +A+          +++ HDI   + PH+  W   YGK+F+ W GT+P L + + 
Sbjct: 62  SAMVSQSAS-------KDCDSIHHDIVGRLLPHYVAWSKQYGKRFIVWNGTDPRLCLTET 121

Query: 123 QLVKKI---SEAVLGKSWGKPAVFRNDREPMFGDGLVMTEGDNWIRHRHILTPAFNPTNL 182
           +L+K++      V G+SW +    +N      G GL+M  G +W   RH+  PAF    L
Sbjct: 122 ELIKELLMKHNGVSGRSWLQQQGTKN----FIGRGLLMANGQDWHHQRHLAAPAFTGERL 181

Query: 183 KAMTKFMVESTTKMLEEWRSLVKSGQSEIEVERQITATAGEIIAKASFGISNCGGGGEAL 242
           K   + MVE T+K++E  R  V  G +E+E+  ++     +II++  FG S+   G E  
Sbjct: 182 KGYARHMVECTSKLVERLRKEVGEGANEVEIGEEMHKLTADIISRTKFG-SSFEKGKELF 241

Query: 243 NNLRALQLTLFKNSSYVGVPFSSLFLYPARTLAAKRLGAQIDRLFLSIISNRK----MSG 302
           N+L  LQ    + + ++  P  S FL        K L  +++RL + II +R+    M  
Sbjct: 242 NHLTVLQRRCAQATRHLCFP-GSRFLPSKYNREIKSLKKEVERLLIEIIQSRRDCAEMGR 301

Query: 303 SGSGYSDLLSRLIG----SEYTGGEALSPREVVDECKTFFFGGHETTALAISWTLLLLAT 362
           S +   DLL  L+      +       + + ++DECKTFFF GHETTAL ++WT +LLA 
Sbjct: 302 SSTHGDDLLGLLLNEMDIDKNNNNNNNNLQLIMDECKTFFFAGHETTALLLTWTTMLLAD 361

Query: 363 SPDWQRILREEIKEVVGDKDI-DFSMLSSLKKMGWVWNEVLRLYPSAPNIQRQAKGDIDL 422
           +P WQ  +REE++EV G   +     LS L  +  V NE LRLYP A  + R A  D+ L
Sbjct: 362 NPTWQEKVREEVREVFGRNGLPSVDQLSKLTSLSKVINESLRLYPPATLLPRMAFEDLKL 421

Query: 423 GDIKIPKGTNIWIDVVAMHHDPTLWGDDVNDFNPSRFQHDTIHGGCNHKMG--YLPFGFG 482
           GD+ IPKG +IWI V+A+HH   LWG D N FNP RF      GG     G  ++PF  G
Sbjct: 422 GDLTIPKGLSIWIPVLAIHHSEELWGKDANQFNPERF------GGRPFASGRHFIPFAAG 481

Query: 483 GRMCIGRNLSSMEYKIVLTLILSTFSLSLSPSYSHSPATLLSLRPAHGIPLILTPL 524
            R CIG+  + ME KI+L  ++S F+ ++S +Y H+P  +L+++P +G+ +IL PL
Sbjct: 482 PRNCIGQQFALMEAKIILATLISKFNFTISKNYRHAPIVVLTIKPKYGVQVILKPL 516

BLAST of Cmc06g0158781 vs. TAIR 10
Match: AT5G24900.1 (cytochrome P450, family 714, subfamily A, polypeptide 2 )

HSP 1 Score: 303.5 bits (776), Expect = 3.2e-82
Identity = 169/510 (33.14%), Postives = 269/510 (52.75%), Query Frame = 0

Query: 22  LFGKVVISCWILPILAHRKLKRNGFEGPSPSFPLGNITEMKKIMKAAAAATAVTNGSSSN 81
           ++G+ V+  W +     R LK  G +GP PS   GN++EM++I   A       + S  N
Sbjct: 27  VYGRAVVEQWRM----RRSLKLQGVKGPPPSIFNGNVSEMQRIQSEA------KHCSGDN 86

Query: 82  NLSHDIHSTIFPHFAQWQNSYGKKFVYWLGTEPFLYIADPQLVKKISEAVLGKSWGKPAV 141
            +SHD  S++FPHF  W+  YG+ + Y  G +  LYI  P++VK++S+     + G+   
Sbjct: 87  IISHDYSSSLFPHFDHWRKQYGRIYTYSTGLKQHLYINHPEMVKELSQTNT-LNLGRITH 146

Query: 142 FRNDREPMFGDGLVMTEGDNWIRHRHILTPAFNPTNLKAMTKFMVESTTKMLEEWRSLVK 201
                 P+ G+G++ + G +W   R I+   F    +K M   MVES   ML +W  +VK
Sbjct: 147 ITKRLNPILGNGIITSNGPHWAHQRRIIAYEFTHDKIKGMVGLMVESAMPMLNKWEEMVK 206

Query: 202 SGQS---EIEVERQITATAGEIIAKASFGISNCGGGGEALNNLRAL------QLTLFKNS 261
            G     +I V+  +   + ++IAKA FG S+   G    + +R L      +  LF+ +
Sbjct: 207 RGGEMGCDIRVDEDLKDVSADVIAKACFG-SSFSKGKAIFSMIRDLLTAITKRSVLFRFN 266

Query: 262 SYVGVPFSSLFLYPARTLAAKRLGAQIDRLFLSIISNRKMSGSGSGYSDLLSRLIGSEYT 321
            +  + F S        +    L  +++      +  R++    +   DL+  ++     
Sbjct: 267 GFTDMVFGS---KKHGDVDIDALEMELESSIWETVKEREIECKDTHKKDLMQLILEGAMR 326

Query: 322 G------GEALSPREVVDECKTFFFGGHETTALAISWTLLLLATSPDWQRILREEIKEVV 381
                   ++   R VVD CK+ +F GH++TA+++SW L+LLA +P WQ  +R+EI    
Sbjct: 327 SCDGNLWDKSAYRRFVVDNCKSIYFAGHDSTAVSVSWCLMLLALNPSWQVKIRDEILSSC 386

Query: 382 GDKDIDFSMLSSLKKMGWVWNEVLRLYPSAPNIQRQAKGDIDLGDIKIPKGTNIWIDVVA 441
            +   D   + +LK +  V  E +RLYP AP + R+A  DI LGD+ +PKG  IW  + A
Sbjct: 387 KNGIPDAESIPNLKTVTMVIQETMRLYPPAPIVGREASKDIRLGDLVVPKGVCIWTLIPA 446

Query: 442 MHHDPTLWGDDVNDFNPSRFQHDTIHGGCNHKMGYLPFGFGGRMCIGRNLSSMEYKIVLT 501
           +H DP +WG D NDF P RF  + I   C +   Y+PFG G R C+G+N   ME K++++
Sbjct: 447 LHRDPEIWGPDANDFKPERFS-EGISKACKYPQSYIPFGLGPRTCVGKNFGMMEVKVLVS 506

Query: 502 LILSTFSLSLSPSYSHSPATLLSLRPAHGI 517
           LI+S FS +LSP+Y HSP+  L + P HG+
Sbjct: 507 LIVSKFSFTLSPTYQHSPSHKLLVEPQHGV 520

BLAST of Cmc06g0158781 vs. TAIR 10
Match: AT2G46960.2 (cytochrome P450, family 709, subfamily B, polypeptide 1 )

HSP 1 Score: 300.1 bits (767), Expect = 3.6e-81
Identity = 197/536 (36.75%), Postives = 292/536 (54.48%), Query Frame = 0

Query: 1   MELICVLIMLIKWMIRVSIMVLFGKVVISCWILPILAHRKLKRNGFEGPSPSFPLGNITE 60
           M L+  L +++  +I +  + +F   +I  W  P +  R+LK  G  GP+     GN++E
Sbjct: 1   MGLVIFLALIV--LILIIGLRIFKAFMILVW-HPFVLTRRLKNQGISGPNYRIFYGNLSE 60

Query: 61  MKKIMKAAAAATAVTNGSSSNNLSHDIHSTIFPHFAQWQNSYGKKFVYWLGTEPFLYIAD 120
           +KK+ +        ++ S  +  S+DI   I PH+ +W + YG+ F+YW GTEP + I+D
Sbjct: 61  IKKMKRE-------SHLSILDPSSNDIFPRILPHYQKWMSQYGETFLYWNGTEPRICISD 120

Query: 121 PQLVKKISEAVLG---KSWGKPAVFRNDREPMFGDGLVMTEGDNWIRHRHILTPAFNPTN 180
           P+L K +    LG   KS  +P   +     +   GLV  EG +W+RHR IL PAF+   
Sbjct: 121 PELAKTMLSNKLGFFVKSKARPEAVK----LVGSKGLVFIEGADWVRHRRILNPAFSIDR 180

Query: 181 LKAMTKFMVESTTKMLEEWRSLVKSGQSE-----IEVERQITATAGEIIAKASFGISNCG 240
           LK MT  MV+ T KMLEEWR      ++E      E+  +      +IIA ++FG S+  
Sbjct: 181 LKIMTTVMVDCTLKMLEEWRKESTKEETEHPKIKKEMNEEFQRLTADIIATSAFG-SSYV 240

Query: 241 GGGEALNNLRALQLTLFKNSSYVGVPFSSLFLYPARTLAAKRLGAQIDRLFLSIISNRKM 300
            G E   +   L+     + + V +P  + +L     +   +L  ++D     IIS+R  
Sbjct: 241 EGIEVFRSQMELKRCYTTSLNQVSIP-GTQYLPTPSNIRVWKLERKMDNSIKRIISSRLQ 300

Query: 301 SGSGSGYSDLLSRLIGSEYTGGE--ALSPREVVDECKTFFFGGHETTALAISWTLLLLAT 360
           S S  G  DLL  L+ +  T G+   +S  E++ EC+TFFFGGHETT+  ++WT +LL+ 
Sbjct: 301 SKSDYG-DDLLGILLKAYNTEGKERKMSIEEIIHECRTFFFGGHETTSNLLAWTTMLLSL 360

Query: 361 SPDWQRILREEI-KEVVGDKDIDFSMLSSLKKMGWVWNEVLRLYPSAPNIQRQAKGDIDL 420
             DWQ  LREEI KE   +K  D    S LK M  V  E LRLY     + R+A  +I L
Sbjct: 361 HQDWQEKLREEIFKECGKEKTPDSETFSKLKLMNMVIMESLRLYGPVSALAREASVNIKL 420

Query: 421 GDIKIPKGTNIWIDVVAMHHDPTLWGDDVNDFNPSRFQHDTIHGGCNHKMGYLPFGFGGR 480
           GD++IPKGT + I ++ MH D TLWG D + FNP RF +  +    NH    L F  G R
Sbjct: 421 GDLEIPKGTTVVIPLLKMHSDKTLWGSDADKFNPMRFANG-VSRAANHPNALLAFSVGPR 480

Query: 481 MCIGRNLSSMEYKIVLTLILSTFS-LSLSPSYSHSPATLLSLRPAHGIPLILTPLQ 525
            CIG+N   +E K VLT+IL  F  +SL   Y H+P   ++++P +G+P++L PL+
Sbjct: 481 ACIGQNFVMIEAKTVLTMILQRFRFISLCDEYKHTPVDNVTIQPQYGLPVMLQPLE 518

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_008439236.12.3e-30399.81PREDICTED: cytokinin hydroxylase [Cucumis melo][more]
XP_004151435.24.1e-28994.29cytokinin hydroxylase [Cucumis sativus][more]
KGN57414.21.6e-28094.27hypothetical protein Csa_010508 [Cucumis sativus][more]
TYK22959.12.5e-270100.00cytokinin hydroxylase [Cucumis melo var. makuwa][more]
KAA0033659.12.1e-26999.78cytokinin hydroxylase [Cucumis melo var. makuwa][more]
Match NameE-valueIdentityDescription
Q2QYH75.0e-8836.42Cytochrome P450 714C2 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP714C2 PE=2 ... [more]
Q9ZW951.2e-8636.04Cytokinin hydroxylase OS=Arabidopsis thaliana OX=3702 GN=CYP735A2 PE=1 SV=1[more]
Q9FF181.2e-8435.26Cytokinin hydroxylase OS=Arabidopsis thaliana OX=3702 GN=CYP735A1 PE=1 SV=1[more]
B6SSW82.4e-8235.59Cytochrome P450 714B3 OS=Zea mays OX=4577 GN=CYP714B3 PE=2 SV=1[more]
Q6NKZ84.6e-8133.14Cytochrome P450 714A2 OS=Arabidopsis thaliana OX=3702 GN=CYP714A2 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A1S3AXX21.1e-30399.81cytokinin hydroxylase OS=Cucumis melo OX=3656 GN=LOC103484084 PE=3 SV=1[more]
A0A5D3DI761.2e-270100.00Cytokinin hydroxylase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold38... [more]
A0A5A7SWW21.0e-26999.78Cytokinin hydroxylase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold23... [more]
A0A6J1CIQ83.7e-22775.80cytokinin hydroxylase OS=Momordica charantia OX=3673 GN=LOC111011538 PE=3 SV=1[more]
A0A6J1HYD53.5e-22575.05cytochrome P450 714C2-like OS=Cucurbita maxima OX=3661 GN=LOC111467387 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT5G52400.15.6e-17558.24cytochrome P450, family 715, subfamily A, polypeptide 1 [more]
AT1G67110.18.8e-8836.04cytochrome P450, family 735, subfamily A, polypeptide 2 [more]
AT5G38450.18.3e-8635.26cytochrome P450, family 735, subfamily A, polypeptide 1 [more]
AT5G24900.13.2e-8233.14cytochrome P450, family 714, subfamily A, polypeptide 2 [more]
AT2G46960.23.6e-8136.75cytochrome P450, family 709, subfamily B, polypeptide 1 [more]
InterPro
Analysis Name: InterPro Annotations of Melon (Charmono) v1.1
Date Performed: 2022-10-13
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002401Cytochrome P450, E-class, group IPRINTSPR00463EP450Icoord: 471..494
score: 32.32
coord: 99..118
score: 24.88
coord: 461..471
score: 51.95
IPR001128Cytochrome P450PRINTSPR00385P450coord: 471..482
score: 36.49
coord: 462..471
score: 57.77
coord: 384..395
score: 40.99
coord: 332..349
score: 39.24
IPR001128Cytochrome P450PFAMPF00067p450coord: 90..499
e-value: 1.0E-81
score: 275.1
IPR036396Cytochrome P450 superfamilyGENE3D1.10.630.10Cytochrome P450coord: 33..524
e-value: 3.5E-107
score: 360.9
IPR036396Cytochrome P450 superfamilySUPERFAMILY48264Cytochrome P450coord: 88..522
NoneNo IPR availablePANTHERPTHR24282CYTOCHROME P450 FAMILY MEMBERcoord: 14..519
NoneNo IPR availablePANTHERPTHR24282:SF149CYTOCHROME P450 FAMILY 72 PROTEINcoord: 14..519
IPR017972Cytochrome P450, conserved sitePROSITEPS00086CYTOCHROME_P450coord: 464..473

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cmc06g0158781.1Cmc06g0158781.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016020 membrane
molecular_function GO:0003677 DNA binding
molecular_function GO:0020037 heme binding
molecular_function GO:0005506 iron ion binding
molecular_function GO:0004497 monooxygenase activity
molecular_function GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen